LOCUS       DAOOOI010000001       125953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_15_length_125953_cov_73.801538, whole genome shotgun sequence.
ACCESSION   DAOOOI010000001 DAOOOI010000000
VERSION     DAOOOI010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 125953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 125953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..125953
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15_length_125953_cov_73.801538"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PKX59_00005"
     CDS             <1..933
                     /locus_tag="PKX59_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOZ89273.1"
                     /translation="QYKIEKLPVVDRSGKLIGLVTYKDIIKFKSRPNSCKDKLGRLRV
                     AAGIGVTADMLDRVASLVNAGVDAIVIDTAHGHSKGVITALKSVKKKFSGLDVVVGNV
                     GTAEGAKLLVKNGADGVKVGIGPGSICTTRVVAGVGVPQLTAVADAAGAIKGSGVPII
                     ADGGIRFTGDIPKAIAAGANSIMAGSMFAGVEESPGETIIFEGRKFKSYRGMGSIEAM
                     REGSKDRYFQDAEDDIKKLVPEGIVGRVPYKGTLAETVYQLVGGLRAGMGYCGAKNIA
                     ELQKAKFIRITQSGVRESHPHGITIVKEAPNYTR"
     gene            1277..3895
                     /gene="clpB"
                     /locus_tag="PKX59_00010"
     CDS             1277..3895
                     /gene="clpB"
                     /locus_tag="PKX59_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOZ89274.1"
                     /translation="MDINKLTIKSQEVLQQAQFLAKSFGQQTVETGHLLKSMLEVDEH
                     VVPFLLKKASVNINSLTQKLDNIIQSYPKVSGGQVYLSTDVNDAMQRAFAALKVNKDE
                     FVSVEHLLLALLSGKDKVAKLLKEEGCNEKDLQNAINELRKGERVTSASAEETYNSLN
                     KYARNLNELARNGKLDPVIGRDDEIRRVLQILSRRTKNNPILIGEPGVGKTAIAEGLA
                     HRIINGDVPENLKSKVIYSLDMGALIAGAKYKGEFEERLKAVVKEVISGEGEIILFID
                     EIHTLVGAGGGEGAMDAANILKPALARGELRAVGATTLNEYQKYIEKDKALERRFQKV
                     MVEEPDTESAISILRGLKERYETHHKVRIKDEAIIAAVELSQRYINDRYLPDKAIDLV
                     DEASSKLRLEMDSVPEELDELNRRIMQLEIEREAIKRENDTKKVNELSEEIAQLNEQR
                     NAVKARWQSEKEVVEKIQDIKEKIENLKLEAEQAERSGDYGRVAEIRYGKVREAEETL
                     SKLMEQLNEMHSKGAMMKEEVDSEDIAEVVSRWTGIPVNRMLQSEREKLLHLEAELHK
                     RVVGQDDAIEAVADAVRRSRAGLQDMRKPIGSFIFLGSTGVGKTELAKALAAYLFNDE
                     NNMTRIDMSEYQEKHTVSRLIGAPPGYVGYDEGGQLTEAVRRRPYSVILLDEIEKAHP
                     DVFNILLQVLDDGRLTDNKGRTANFKNTIIIMTSNIGAHLIQENFENIDERNRDEVIA
                     KTKNNVFDLLKKTIRPEFLNRIDEVIMFNPLTRNQIEEIVRIQFNQAVDMLAKNDIKL
                     SITDRAIEWVAQLGYDPQFGARPLKRVLQKKILNELSKQILAGTINKEDVILIDLDKK
                     KNFIFENEKTHTEIKA"
     gene            complement(3930..4229)
                     /locus_tag="PKX59_00015"
     CDS             complement(3930..4229)
                     /locus_tag="PKX59_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOZ89275.1"
                     /translation="MKKAWEKVPDFLKNKYAICIIAFVVWLAFFDKNDFYAQYRFRQQ
                     LNTLKNDKSYYADELEKSKSDLNELISSPANLEKFAREKYLMKKDDEDIFVFVEK"
     gene            complement(4351..5481)
                     /gene="tgt"
                     /locus_tag="PKX59_00020"
     CDS             complement(4351..5481)
                     /gene="tgt"
                     /locus_tag="PKX59_00020"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOZ89276.1"
                     /translation="MHFSIEKYDKESKARSGRIETAHGIIETPIFMPVGTAGTVKSIN
                     QRDLKQDVKAQIILGNTYHLNLRPGIEVLHQAGGLHKFMNWSLPILTDSGGYQVFSLS
                     ETRKITEEGVKFNSHIDGSKHFISPEVSMDIQRFIGADIIMAFDECTPYPCEHRTAKK
                     SMEMTHRWLQRCISHLEKSPAKYGYEQTLFPIVQGSVFKDLRTESSKVIADAGAKGNA
                     IGGLSVGEPAEIMYEITDVVTDILPVEKPRYLMGVGTPANILESIALGIDMFDCVMPT
                     RNARNGMLFTAEGIINIRNEKWKNDFSPIDESGNCFADTDYSKAYLRHLFISKEMLAA
                     QLATLHNLSFYLWLVTEARKRINDGSFLSWKNLMVKKVSQRL"
     gene            5740..6741
                     /locus_tag="PKX59_00025"
     CDS             5740..6741
                     /locus_tag="PKX59_00025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ89277.1"
                     /translation="MSEIEPPVSIIICARNELKNLKAYLQQVLEQDYPRFQVIVVNDC
                     SWDESEKYLEELSATYTNLKVVSIKEQERYQHGKKFALTIGIKSALYDHLLLIDADCY
                     PASNQWIRLMMQSYNAKTRIVIGYGAYIKTSSLLNRWVRFDTAFNAMQYLSFALAGNP
                     YMGVGRNLSYQRSLFFENKGFARHQHIMSGDDDLFINDTATTENLSVQINPDSFTYSQ
                     PKTDFIDWFRQKKRHISASRLYKFKHKMLLGIYYFSHISFLVLFIALLIIQFKIEYVV
                     TAFSLRLVFQMIIFGSCLKKLKEGDLIPLIPIFDIWIAVVYPLIAISNRLIKNKTWK"
     gene            6732..7385
                     /locus_tag="PKX59_00030"
     CDS             6732..7385
                     /locus_tag="PKX59_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOZ89278.1"
                     /translation="MEVKSPKTAKAAQDKTEVNPNLSDKAVVDYQLVREAVDHGNQKA
                     YAELMSRYKDSIYFMLLKMVNNRDDADDLTIEAFGKAFKNIHQYTPDYAFSTWLFKIA
                     TNNCIDYIRKKRKLMLSIDKGYENENGEDVALEIKSDTIGPEEKLMKKQKMETMREVV
                     DKLKPRYRTLIELRYFQELSYEEIATNMDLPLGTVKAQLFRAREFLYQIMKNSEHRP"
     gene            7389..8024
                     /gene="rsmG"
                     /locus_tag="PKX59_00035"
     CDS             7389..8024
                     /gene="rsmG"
                     /locus_tag="PKX59_00035"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOZ89279.1"
                     /translation="MSSGVSLLQRYFPELPLEKQEKFVLFAELFKVWNEKINLVSRKD
                     IDQFYLHHLLHSLVIAKFIDFGKGTKVMDIGTGGGLPGLPLAIYFPETNFYLVDSIRK
                     KTEAVKEMANQLSLSNVTVFNKRAEDVSEKVDFVVSRATAPLSDLVKWSRGRINSKGK
                     ASIPNGLICLKGGDLTEELKPFENKLFTENVTAFIQEEFYKEKKIIYLPLV"
     gene            8091..8252
                     /gene="rpmH"
                     /locus_tag="PKX59_00040"
     CDS             8091..8252
                     /gene="rpmH"
                     /locus_tag="PKX59_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOZ89280.1"
                     /translation="MVRTYQPSKRKRKNKHGFRERMATANGRRVLASRRAKGRKKLTV
                     SSESKVRGK"
     gene            8339..8887
                     /locus_tag="PKX59_00045"
     CDS             8339..8887
                     /locus_tag="PKX59_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89281.1"
                     /translation="MNFKSGLFYLSIILLFTACQKDYNDPGTGPSQYYGTRSGVSIRA
                     ILLAQMPATDPSGNSWDVADSVDTAGRADIYYRFKDIDSIDYPEFVQNFHFDNVNPDS
                     LPLPYFLTTPYPLRAIGSNVQFDIYDFEKSLSSPAIDSTLMKSFNFSVVAGDSLIENP
                     YPDSLVLAEDGYVVLLYLNWKK"
     gene            complement(8962..9921)
                     /locus_tag="PKX59_00050"
     CDS             complement(8962..9921)
                     /locus_tag="PKX59_00050"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89282.1"
                     /translation="MKKLFTLLFVILICYIQGQAQIYSNGTIINHPGVGQGGADISCF
                     HHGLASLGFGQQLLEGYRIADDFNIPAGQIWTIDSIRFYAYQSNSGIVSTINAVNCEI
                     FNGSPASGGTIVLGDTITNLLQNTRFSNIYRTVDGQFNSVSRPVMYSVIVPQSAWTLT
                     SGNYWLSWQSGGNPSLSGPWVPPLTDTLNTTTGDGLLYIPDSSMWLPALDVALSTPQG
                     FPFEIYGTITTSINTIEVEDALTILPNPSKGNFMINASFANRCNLEINIMDLQGRSIY
                     SENVSDVSVLTKNVVLNDVSKGIYLLKITTDTGKTSIKKITIN"
     gene            complement(10073..11029)
                     /locus_tag="PKX59_00055"
     CDS             complement(10073..11029)
                     /locus_tag="PKX59_00055"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89283.1"
                     /translation="MKKLFTLITAVALGSLTSNAQIYDNGTIVNNPVLGVGGADVSSL
                     HDGLNSLGMNHASGLGYRVADDFIIPAGQTWTIDSIAFLAYQTNSGNASTITDVNCAI
                     FNGSPAIGGSIVLGDTITNLMQSTYFSNIYRTADAAFTNTARPVMRNIVVPQSAWSLP
                     AGTYWVAWQTGGTLTSGPWAPPLTNANMATGDAIQFNPDGSIWNPILDGGATADPQGL
                     PFQIYGTVTTSINSIEVEDALTILPNPSKGNFMINATFSNRCNLEINVMDLQGRSIYS
                     ENVSDVSVLTKNVVLNDVSKGIYLLKLTSDSGKVLTKKIVVD"
     gene            complement(11148..12143)
                     /locus_tag="PKX59_00060"
     CDS             complement(11148..12143)
                     /locus_tag="PKX59_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOZ89284.1"
                     /translation="MKIVIPVAGIGTKLRPHTHTQPKTLVPVAGHPILAHIIDYFVQS
                     GYDEFVFIIGYMGEKIEEFVKSKYPSLKAAFVLQEPREGTGHSVWFARNHVHAEDEML
                     IVLGDTIIDFDINVFKQAEQSLLGVKKVDDPRNFGVVELNDEGFVSKLHEKPVIPKSN
                     LALVGIYFIKQAGHLFEALNYIIENNLKTRDEYHLTDAMMRMIEEGEKIKVFHVGNWF
                     DCGNKESLLETNAILLDRIAETNPKPQKFPNTIIIPPVSIAYNCDIADSIIGPHVSIG
                     EHTIVKYSIIKDSIIGSYSQLESAVLHRSVIGSDAFLKGLSQSLNIGDSTEIDFS"
     gene            12255..13310
                     /locus_tag="PKX59_00065"
     CDS             12255..13310
                     /locus_tag="PKX59_00065"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HOZ89285.1"
                     /translation="MLNKITELLGKDADKLLNHVCKTVPKETLHQPGNDFVDRIFIPS
                     NRSNQVIRSIQSLYNHGRLNGTGYMSILPVDQGIEHSAGASFAPNPMFFDPENIVKLA
                     IEGGCNAVASTYGVLASVSRKYAHKIPFIVKINHNEFLTYPNKFDQIMFGTVDEAWNM
                     GAVAVGATIYFGSEESSRQIVEVAQAFERAHELGMATILWCYLRNPGFKKDGIDYHTA
                     ADLTAQANHLGVTIQADIIKQKLPTNNGGYTAINFGKTHKDVYAKLSSEHPIDLCRYQ
                     VLNCYNGRMGLINSGGESKGATDMAEAVTTAVINKRAGGQGLISGRKAFQKPFKDGVE
                     MLNAIQDVYLSKDVTLA"
     gene            13371..14216
                     /gene="accD"
                     /locus_tag="PKX59_00070"
     CDS             13371..14216
                     /gene="accD"
                     /locus_tag="PKX59_00070"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOZ89286.1"
                     /translation="MTWFKRIKEGITTSTREKKETPEGLWYKCPSCKAIVPSLEHEEN
                     KYVCPKCNYHDRIGSEEYFAVLFDDNQFTEFNTELTSADPLGFVDTKKYTDRLRDTIS
                     KTKLKDAMRTAVGKVNGNDLVVACMDFQFIGGSMGSVMGEKIAGAIDYSIQHKMPLMI
                     INKSGGARMMEAAFSLMQMAKTSAKLTRLADAGLPYISLLTDPTTGGVTASFAMLGDL
                     NIGEPGALIGFAGPRVVKETIGKDLPKGFQTSEFVLEHGFLDKIVDRRQLKDIIGQIL
                     SMFKN"
     gene            14334..15209
                     /gene="ygiD"
                     /locus_tag="PKX59_00075"
     CDS             14334..15209
                     /gene="ygiD"
                     /locus_tag="PKX59_00075"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HOZ89287.1"
                     /translation="MYRREFLSSIAGIGAIGTLSSFKNITDDLSNSDKMPVLFIGHGS
                     PMNGIENNEFSNKWKKLGQSLPKPKAIVVVSAHWLTKGTWITAMNHPKTIHDFGGFPQ
                     ALYNVQYKAPGSPELAQETKNIIKSVNADLDHDWGLDHGAWTVTRRMYPDANIPVLQI
                     SIDINKPASFHYQLGKELASLRTKGVLIIGSGNMVHNLGKIAWDKIDEVGFGYDWAVE
                     MHQLFKSKISDNDHQALINYASLSKSALMAVPTPDHYFPLMYVLGLQQKNENAEFFND
                     KLVAGSINMTSVKFG"
     gene            complement(15226..16203)
                     /locus_tag="PKX59_00080"
     CDS             complement(15226..16203)
                     /locus_tag="PKX59_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOZ89288.1"
                     /translation="MLHKSFFTAIVFLFLSTSLFSQKRIKIPVLDYGSVDEFVLKLKP
                     EQCVNIDTLAEVISREFDRPHFRFRAAFRWITENIAYDLKNQRHPQKANLNANDVFKN
                     KTALAEGYANILNAICAKMNITCLSVNGYLRRNQLPGTKIKKPNHIWSTVKLYDKWYL
                     MDVMMASGYFEKGDKIFTKRFTERYFALHPRQIILTHLPLNGNRQYLDTIVDKNTFLN
                     YPEVYDFFFDNGIYALRPADGVLPVYKGLKKRFYFKSMDSLAIGKIEIRDGKKSEKIQ
                     NTFTQVDSLVYFDYVFESNKKRTLDVFVDGLPLCTYFIVPDKKKKRSEY"
     gene            complement(16267..17421)
                     /locus_tag="PKX59_00085"
     CDS             complement(16267..17421)
                     /locus_tag="PKX59_00085"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOZ89289.1"
                     /translation="MYPTLSDALHDLLGINIPLPIQTFGLILALSFFLAAYILILEFK
                     RKEQNGLLKSFTVTTIKGKPASTAELFWSGVVGFLVGFKLLDAILNYSDLVNDPQGFL
                     LSTRGNVIGGILLGAYSVWSKYKEKEKQKLAVPQTVTEVGYPHQFVMNITIAAAVAGL
                     IGAKIFHNLENIDDFLKDPVDALISFSGLTFFGGLICGALAVIWYSNKKLNVPPLVMC
                     DVAAPALMLTYGTGRLGCHFSGDGDWGIVNTLAKPSWFPFPDWMWSYNYPHNVIGEGV
                     PIAGCVGKHCYELIPPVFPTPLYEAIACILLFFVLWKLRTRIVTPGILFFVYLLFTGV
                     ERLAIEQIRVNTKYHIFGKAITQAELIAVACIIVSIVGITLLRRKNSGSS"
     gene            complement(17434..18765)
                     /gene="mtaB"
                     /locus_tag="PKX59_00090"
     CDS             complement(17434..18765)
                     /gene="mtaB"
                     /locus_tag="PKX59_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOZ89290.1"
                     /translation="MTRESTVAFHTLGCKLNFAETSTIGRQLQAAGYKKVSFSDTADV
                     YVINTCSVTENADRECRQHVNRALAANPEGCVIIIGCYAQLKPDAIATIPGVSLVLGA
                     SEKFNIPHYLQKLKKQTEAQVFSCDIADHHQFTSSYSEGDRTRVFLKVQDGCDYGCTF
                     CTIPLARGASRSNTIENVIENVKQIVDRGAKEIVLTGVNLGDFGKLPGEKKHATEFFQ
                     LVSALDALDADIRFRISSIEPNLLTEEIIELVSCSKKFMPHFHIPLQSGSDKILKLMR
                     RRYLTSHYTSRINKIKKLMPDACIGVDVITGFPGETEEDFLQTYKYLNEADISYLHVF
                     TYSERANTPAIEMKPVVPVAERKRRTNMLRILSEKKLGIFYRSQEGKTAKVLFEHENK
                     NGIMSGYSENYVRVSTPFNNEMVNKVIECKIGATDNDSHEVNCTILESITA"
     gene            18871..20622
                     /locus_tag="PKX59_00095"
     CDS             18871..20622
                     /locus_tag="PKX59_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ89291.1"
                     /translation="MSTSGTAFNWKVLNRIMFYVKPFRFLFVATVVITVLLAVLAPLR
                     PWLTQQALDIDIAKHDIKGLTRTVAFMFGVLLLQSLLQFVYTTLTNRLGQEVILSLRN
                     NLFKKVSGFGLAYFDKTPLGITITRLVSDMETIADIFSDGLILIISDMLQVVVIIAVM
                     FYIHPQLAAISLSTIPVLLIATRVFQKNIRKTFNDVRIQVAKLNEFVQEHLVAMRIVQ
                     LFNREEEELNKFHEINAKHRDANIRSIWYYSLFFPVVEILSAVSIGLIVWYGGLNVLQ
                     SSISLGLLVAFIQYINQLFRPIRELADKFNTLQMGMVSSERVFKLLDEPFTEVNSGTK
                     SADNIIGKIEFRDVWFAYKNEEWILRGLSFIIPSGQSVAIVGSTGAGKTTIVSLINRL
                     YEIQKGQILIDGVDIRDYDLYSLRNAIGVVLQDVFLFSDSIKNNITLYREQDDIVIEA
                     LLKQTGAWHFVEKLPGQLHYNPGERGGLISTGQRQLLSFARVLAHQPKLIILDEATSS
                     VDSKLEQLLVNATEMLTKNRTSIIIAHRLSTIEKADRIIVLHKGAIAEDGNHQNLLER
                     KGIYAHLHDVQFKQPSA"
     gene            20670..21296
                     /locus_tag="PKX59_00100"
     CDS             20670..21296
                     /locus_tag="PKX59_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89292.1"
                     /translation="MKTFSGLITVLIFYSVQAFSQGSALMKEDAVQVNKFNDGVYFTL
                     TDLQHNTPTVTKDHLIKSYYDQSDFSIGQWATTQNLFYTAKDEHKLKVNRDSLWGYVE
                     NGTPYIYLNDRFHRFSTAGAISVFTESYPNIKAGLAPVVTEARSSSVINLFILSTGKI
                     VEFTPENVADAIEGNEQLYKEFNALKSWKQKRKKMYRFVERYNETEAF"
     gene            21318..22811
                     /locus_tag="PKX59_00105"
     CDS             21318..22811
                     /locus_tag="PKX59_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89293.1"
                     /translation="MKKLKYFIFVLLILISFYNLTSCSQKKEAISDLPGFVQIKNNQF
                     VLNGNPYFPLAVNYRVALQTNGKQLWPSAYKGYDDGNRYRFTNRDSSLEELNNDFQLI
                     ADMGFNSIRIVGIGEQYSVAKNSDELYIRADIGNEKDTMLLFNSQETYNQYFNALNDM
                     LQAANKAGLRVVLLSRLFHEVPATEKHWGRIMKHFQNDTTILAYDFFNEPLYFDSLER
                     NKTEPYYITKAWYELAKENAPHQLTTIGLTGIREVFEWDPNTLNVDFISYHPYEYEPE
                     QVRNEIYWYGNYVKKPWILGETSLPADNDSVPYSIQKNFAEATFNQTCNCGGIGYTWW
                     QYKDVDWFDFHSNFMGVVNRKGATLTSSGKFVIGTPKSMIDFIKHAKPLKDSTKCLKL
                     ENYYNYSNANVCRLIGKLVGPSGEPIAGGVIMAWNQYWSSSFHTVTKEDGTFELKGSF
                     PFYHWMASATMMTMIRGDVLPDTAKINVHEIPTLDMGILKVAKLKLD"
     gene            complement(22808..24181)
                     /locus_tag="PKX59_00110"
     CDS             complement(22808..24181)
                     /locus_tag="PKX59_00110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOZ89294.1"
                     /translation="MPIYIWDEARLASNAIEMSITNDIIVTHYEGKPDLWNTKPPLLI
                     WMQAGLIKLIGINELAIRLPSAIAGLITCFALFFFLKRHTKNYWFGFIAALVLVTSQG
                     YVGYHGTRTGDYDALLILFITLFSFSFYNYLSIGKIIHFKLFFLYLTLAILTKGIQPL
                     IFLPGLLLIGFLNHKDSGIKFQFKSTVFYSIFCIAVVAGYYFIRETQNPGYLKAVIEN
                     ELGGRYLKTIESHDHGFTFYYDNILNKQFNYWYLPMFIGVFIGLLNKNTTVKKLSIFC
                     MILTLFYFLVISCAQTKLEWYDLPMFPFMAILCSISIYELFLLVKHIPQFAERLKFNI
                     APYIILFFIFLFPYRAIIDKVYFPKMYDYNNESIPFILKNLPSEVKTCVICWDNGYGP
                     QINFYITKLSKQNITCYYKYYKDLSPGDVAIASQEYEKEYIEANYKTTLLLERAAAKM
                     YKIENRN"
     gene            complement(24279..26069)
                     /locus_tag="PKX59_00115"
     CDS             complement(24279..26069)
                     /locus_tag="PKX59_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89295.1"
                     /translation="MYPYKNVIALSDNLIGTMPIYAIFRVFNVDRETSFQLWIIVLFL
                     LNYWSCFYVLKKFGINNLIAASCAFIYGFGLYNHGQIFHIQVFPRFIAPLVIYWSIRF
                     IQTLKSRYFTFLLLGLAYQFYCGIYLGFFLAYFIMFLFIAAFIVNLKLPFELNKKALL
                     NYTLSIIGTIIVLLPLFLPYIHSADQVGTRSYEYSLNSLPRLRSYFFTSPASVTWNFL
                     YPFTAFKFINWWNHLLFPGIIPWLGVVASPFVFIALKKNSENSKILLLIIITFVLSLI
                     FCLNIKNYSLYKIIYLIPGFSSMRSVDRIMNIQILLFVLLTAFSLHFLIKHIRLKVLF
                     ACLLPILVLFDNYFIPEPHKSISKSEAQANVQYFKSIISEQYNSKYKAIAFFPVDLAL
                     TKAENKNFTAVSQTLSIMLACQDLHIKCVNGYSGFNPYNFLQFFEYPDSSSLKTWCND
                     NKISDSDIQIMNDFEVGGFYLDTMQIMTNDSLYWCLDKNYNDLIMANRKIPQSWETFL
                     ALKFNSGKTLISSLDQVYLKVDPYNKRLLGYKNSFLNSDLVNIEPYQQQRSVIKIYNK
                     YATVDKETKEVRLTDEINIEQSTFEIKILK"
     gene            complement(26313..27029)
                     /locus_tag="PKX59_00120"
     CDS             complement(26313..27029)
                     /locus_tag="PKX59_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ89296.1"
                     /translation="MIQKLSIIIPAYNEARTIHLILDKVLAVKLTNNISKEIILVNDC
                     SKDTTKEVIEKYITDHPEADMRLFNQPVNMGKGAALHKGIELATGEYLIIQDADLEYD
                     PEEYNILLKPILTGFADVVYGSRFLGGKPHRILFFWHSIGNKFLTFCSNMFTNLNLTD
                     METCYKMFNTKMVQSLKLKEKRFGFEPEVTAKIARIPNVRIYEVGISYYGRTYAEGKK
                     IGWKDGFRALWCIVRYGLSH"
     gene            complement(27441..27590)
                     /locus_tag="PKX59_00125"
     CDS             complement(27441..27590)
                     /locus_tag="PKX59_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HOZ89297.1"
                     /translation="MELLSDLFRFIIQRKKYWLAPLIIVILLMSVLIIAGSNPAFAPF
                     IYTLF"
     gene            27703..29298
                     /locus_tag="PKX59_00130"
     CDS             27703..29298
                     /locus_tag="PKX59_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOZ89298.1"
                     /translation="MLLKQEIEKRRTFAIISHPDAGKTTLTEKLLLFGGAIQVAGAVK
                     SNKIKKTAASDFMEIEKQRGISVATSVMTFFYKNCQINLLDTPGHKDFAEDTYRTLTA
                     VDSVILVIDSVKGVEPQTEKLMEVCRMRNTPVIIFINKMDREGQNPFDLLDELEEKLF
                     IHTRPLSWPISSGSTFQGVYNLHNKSLMLFRANQKASDSDVLPIKDMQDPLLDKQLGR
                     YVDQLREDVELIEGVYEPFNKQLYSEGLLAPVFFGSGINNFGVKELLDTFIEIAPEPK
                     SRQTDQRTVTPEESKFSGFVFKIHANIDPRHRDRIAFLRVCSGMFERGKFYHHVRLNK
                     DLRFNNPATFMAQEKSIVEQAFPGDVIGLYDTGNFKIGDTLTEGEELMFKGIPSFSPE
                     IFKELINTDPMKTKQLEKGIEQLTDEGVAQLFIQNGGNRKIIGTVGELQFEVIQYRLQ
                     NEYGASCKFITQNISKACWLTSKDKNKLDEFMRFKMNNIAFDKDQNPVYLADSQWMLD
                     QMIKNYPEVQFHFTSEFKMEVAV"
     gene            complement(29295..29870)
                     /locus_tag="PKX59_00135"
     CDS             complement(29295..29870)
                     /locus_tag="PKX59_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89299.1"
                     /translation="MFSSEEIQHAISADFFNIKRAITDKVYSNFAELGTMAKEKLESD
                     FKIESTIINRSKIYRGENLEGLPWINCDAPGWFNKEETFAIRFLFWWANHYSVTLHLS
                     GKFLNMLDYDKMQSSKSENVLLCINEKPWRYDLHKDNYIPLAEIKDVKPRCRKMNFIK
                     LSIAYPIATLHDFNSQNKKALMCLIGLLRQN"
     gene            29930..30493
                     /locus_tag="PKX59_00140"
     CDS             29930..30493
                     /locus_tag="PKX59_00140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOZ89300.1"
                     /translation="MKLTIISASVREGRKSHRAALFFKNFLIKKYQADVSIADLNELN
                     FPIFEERLRFQKNKSPEVVQFAENIKQSDGVLIITPEYNGGYPASLKNVIDLMYDEWR
                     RKPIAIATVSDGMFGGMQVITSLQFILWKIRAWTVPAMFPMPQIDKNFDVDGTPVDVA
                     GTEKRATVFCDEWMWCIEANRRMQLNH"
     gene            30557..31189
                     /gene="msrA"
                     /locus_tag="PKX59_00145"
     CDS             30557..31189
                     /gene="msrA"
                     /locus_tag="PKX59_00145"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOZ89301.1"
                     /translation="MTSLIAIILLTGCQNQAQSLREISNSTNMQSNIATDTATFGTGC
                     FWCTEAVFESLEGVISATSGYSGGNVADPTYEQVCSGNTGHAECIQVVYDSKKISYDD
                     LLQVFWESHDPTTLNKQGADVGTQYRSVIFYHNPQQKQLAESYKQKLNNENTFGKPVV
                     TEITAATTFYPAENYHQEYFKLNGHAPYCQFVIQPKVEKIRKIFSSKIKK"
     gene            complement(31196..32083)
                     /locus_tag="PKX59_00150"
     CDS             complement(31196..32083)
                     /locus_tag="PKX59_00150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOZ89302.1"
                     /translation="MEHFRESLLILISTPIYIIVIGAEIFFSAFHHLQLYSKKGTLVN
                     LYLMLLNMGLDIIMRTASLLILNYFFDFRIINSYPTAWIYWLALFLAEDFMYYWLHRI
                     DHFCRLFWAVHVTHHSSEEFNLTVGFRSSVLQPLYRFIYFIPLSIVGFKAIDIMFIYS
                     ATQIFGILVHTQTVGKLGWLEYFLVTPSHHRVHHASNVCYLDRNMGMVLIIWDKLFGT
                     FQRELENEPIKYGLTNNLHSNNPGKIVFHEWQAIAHDLKKKTSVKNKIMYLLGPPGWS
                     HDGDCKTSAQLRKALLKKK"
     gene            complement(32105..33358)
                     /locus_tag="PKX59_00155"
     CDS             complement(32105..33358)
                     /locus_tag="PKX59_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOZ89303.1"
                     /translation="MPSVCFYFQVHQPFRIKHYSVFDIGKDHFYEDGEKNKWIMDKVS
                     EKCYLPANKKMLELIKRHKGKFRISYSLSGTVIEQMELFRPDVLQSFVDLSKTGCVEF
                     LSETYFHSLSFLYNKEEFERQIKEHDQKIEHYFKQKPTVFRNTELIYNNELAAFIEKM
                     GFKGILCEGVDRLLKDRHPNHLLKPTGTKSIKALLKNYRLSDDIAFRFSDKNWSEWPL
                     HADTFASWVHKVAGSGDVINLFMDYETFGEHQWESTGIFDFMDHMPREILKHPDFGFM
                     TPSQVVDAYTPRGVYDAHEFISWADTERDLSAWLGNNMQQEALNKIYSISDRVLGSGN
                     KDLIDVWGKLQTSDHFYYMSTKFWSDGDVHKYFSPYNSPYDAYIFYMNAFSDFESTLT
                     EEIKPNRKSAAKKPAAPRKKKPAAE"
     gene            complement(33521..35329)
                     /gene="rpsA"
                     /locus_tag="PKX59_00160"
     CDS             complement(33521..35329)
                     /gene="rpsA"
                     /locus_tag="PKX59_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOZ89304.1"
                     /translation="MSEVKHNVPPTDFDWDNVGKTADIYKSDDRQRMEAMYDQTLNTL
                     TEHEVKMGSIISISDKEVVVNIGFKSDGMVPLSEFRHMPDLKVGDEIEVYVETREDKN
                     GQLILSHKKARALKSWERVNEALEKDEIIQGYVKCRTKGGLIVDVFGIEAFLPGSQID
                     VKPIRDYDVFVGKTMEFKVVKINHEFKNVVVSHKVLIEEELESQKSEIMSKLEKGQVL
                     EGTVKNITSYGVFMDLGGVDGLLHITDISWGRINHPDEVLKLDDKINVVILDFDEDKK
                     RIALGLKQLTPQPWEALDQNIQVGDKIKGKVVVLTDYGAFIEVIPGVEGLIHVSEMSW
                     SQHLRSPQDFLKVGDEIETVILTLDREERKMSLGLKQLMPDPWVNIMEKYPVGSKHTA
                     KVRSLTNFGVFVELEEGIDGLIHISDLSWAKKIKHPAEFTKVGENIEVVVLEVDSEHR
                     RLSLGHKQLEENPWDTYETIFRVDTVHSGVVGKVTDKGATIIFPSFGVEGFAPYRHLM
                     KENGTPAKAEETLDFKVIEFSKENKKIVVSHSRLFEEAKASEKMARDNEKAKDESDAF
                     KSVKKIKDSQEKTTLGDLSVLSALKDKMKKNEAENN"
     gene            complement(35566..36576)
                     /locus_tag="PKX59_00165"
     CDS             complement(35566..36576)
                     /locus_tag="PKX59_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89305.1"
                     /translation="MLKKILIGFVIIIGLLCVGSLFISNNYKIERDIYIKAPASVVFN
                     QINNLKNWSKWNTWLKMDTTMKVTYNNIEAGVGAQYQWTSANKNVGNGSMQIKKSVQD
                     QSVESELLFEGEGNAVNGFNIQPTGDSVKLTTYFESEVGWNPLHKYMMLMFKGMMIDM
                     TDNGLKAIKELAENMPAQPGFEFNIQQVEMPSSIPYLATRCTSGLDSISYFIGSSYGL
                     IDTEMKKQKLEMSKTVHPFAIYYKWENNVFEYDVCMPLAAAGKSAGNIVAGEVKTGKY
                     LMTQYFGNYDNSNKAHEALVNYAKDNSIKISDAPLEFYITDPSVEKDTAKWQTDIYYQ
                     IN"
     gene            complement(37286..38737)
                     /locus_tag="PKX59_00170"
     CDS             complement(37286..38737)
                     /locus_tag="PKX59_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89306.1"
                     /translation="MPLSVWSAPYLDYNERCKNAYQLIFELRFNQALDSLDAEKKEHP
                     DNLIPGYLYHYHLFIKTFIGEEQATFNELKHNHEIITGQIKAMPMLSPLRNFCLAECY
                     LMTALSKLKFKEYLTAALQVKKAYSLLTENQKKFPEFELNLKSLGVLHAFIGAVPENY
                     QWLVKIAGMDGSVSNGIKELEQSFMKTREDSDAFHFMNKEIAFTYIFTRNQLKPDPEW
                     VIKFLSGLKLGNEPLDLFFSTNIYYSTGRSAQIIPLIENYHADDEVYPIHYFQFLLGM
                     SKLDKLDFSSFSNFINYVKLHKGNSFIKAAYQKMAWIKLLQNDKAGYDAMITEVKNVG
                     NDFTDEDKQAQNELITHQEPNTYILKIRLLFDGGYYQEALKLLAKGSNEAKTAKDQLE
                     YKYRLARVLDKLEMTDRAKEFYLKTIDHGQDLKFYYAAASCNFLAQMYEKEKDYVLAE
                     KYYKKCLSLRDHEYQNSLDQKAKAGLNRIAGSR"
     gene            38926..40077
                     /gene="nhaA"
                     /locus_tag="PKX59_00175"
     CDS             38926..40077
                     /gene="nhaA"
                     /locus_tag="PKX59_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HOZ89307.1"
                     /translation="MIRGKIINPIRQLAEQGKLAGLLIIVLTLVSLLLSNLSATSEYA
                     GLWHSHIHLLGYDVSVLHIINDGLMVVFFLMVGMEIKRELYVGELANRRNAMLPVIAA
                     FGGMLAPGIIYAAFNISDTHLLHGWAIPTATDIAFSLGVLSMLGNRVPFALKVFLTAL
                     AIIDDLGAIIIIALFYTEPENFHLSFLVSSLLIVGALMFLSNKGFFNLFLWIIAGIVV
                     WYFMLLSGVHTTIAGVLIAFTIPLNKLTSFENKLHLPVNYFILPVFALANTAIVLSID
                     NVSGLFSTVGLGVSLGLLIGKPLGITLASWIAVKNKWCSLPGKINLKLIFGAGLTAGI
                     GFTMSIFIASLAFKEPLLLDTAKIAIIAGSVLSGLAGVLFLKSNLKAKA"
     gene            complement(40085..40771)
                     /locus_tag="PKX59_00180"
     CDS             complement(40085..40771)
                     /locus_tag="PKX59_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89308.1"
                     /translation="MLSINVFSQKGVATIGFQFKPIFNNDFFRTGAQSATQGTAEVTI
                     QPNSGYCAGMVIRRGFTERLSIETGINYARRNYQIQISDSALSHKRNFKFIGYEIPIS
                     ALVYIRLGQKMYIDASLGNSFDFYPSDIYIGDDYFESEGLRKSWIKPAIIANVGAEYR
                     TEKSGYFYLGVSFHRPYSFIYRSRTLITGRNYKVFESDISGNYLTVDIRYFFHEDPLN
                     KQRKRYPKDD"
     gene            complement(40863..42191)
                     /locus_tag="PKX59_00185"
     CDS             complement(40863..42191)
                     /locus_tag="PKX59_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89309.1"
                     /translation="MSLKLLNTFALLLIAVQSYAQQVKFQQEWLDNPAKYSSEIEDIF
                     TSDSVRAFQKEEYKTFTILENGYAKSQIKNTADWKGIPSGFYADTIIFIYTQYPKNIN
                     FWLTNYHTLLAQRLKNLFKLSSDLNNEHTVFKILLQTECNTDEEAKKMFHGIAIHLAP
                     VKSESVKQHENIIKQQVYDDSLKNIKHSDQNTIATRKINSFIANNGGITDSVVFKIFS
                     RHPEWDNCLVIMDWTGSMYPYGAQAVLWHSLNLKQSGIKYFVFFNDGNMEKRKKIGKT
                     RGVYFEKAENINKIIGLLSKVKAKGNGGDPEENDVEAILKGIQKYPDFKNLILIADNN
                     SCMRDYCLVKDIKVPVKVVLCGTYSGINPQYINLAYKTKGSIHTIEDDISDFYNRTNT
                     DATFMIDGSEYRYNTKRDLFEYLTKPDKDDPHFCEPFYKKKKCKCEKLNY"
     gene            complement(42195..42737)
                     /locus_tag="PKX59_00190"
     CDS             complement(42195..42737)
                     /locus_tag="PKX59_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOZ89310.1"
                     /translation="MLFLCRFKLIEKMEDRQVILNHEQIQQKLRRIACQIYEDNISEK
                     NIYIAGIVRSGYVMAAKLRDIIVESYPLKVNMLEVIIDKENPLHPTIKGIDNTALLKN
                     KVVIIVDDVLNSGKTLIHSLRPFLEADAKKISTALLVDRDHKRYPVATDFVGLTLSTT
                     KHEHIVADLKKGKKDVVYIS"
     gene            42833..45706
                     /locus_tag="PKX59_00195"
     CDS             42833..45706
                     /locus_tag="PKX59_00195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HOZ89311.1"
                     /translation="MMFQPKQKIIKSTVLAFILMFFAFTVVHAQKQFRFPAGIASDDY
                     EKGILIVRIKSDYRNLCNDSDINSSDLLKIFNKLSVTEVKKKFPNHIQPKETKTSFGF
                     ALTDLSLIYTLKFNTDIPVEKAASLMLSCKEVQYAEPAYIDKPLYTPNDTLATPQYQY
                     HHVNIHTFDGWDIQKGDTNIVIGITDTGIDTVHVELINQLKYNYADTINGIDDDGDGY
                     IDNYRGWNVAFNSNDVQGSVPLHGNFVAGISNAEVDNITGIAGVGFKTKFLPVSCSPG
                     SNVIVNGYDAIVYAADHGCSVINCSWGGFGSSQFSQDVINYTTFNKNALVVAAAGNAN
                     NDAPFFPASYQYVLSVGGTDSADVKWTSSPTSGSSFGSYVDVTAPGDKIFSIFPTGVN
                     PPYWYSGGTSEAAPQVSAIAALVKAQFPTLNALQIGERIRATCDDIYSIPGNANFINK
                     LGKGRVNLLSALTQPATSVRAFDIENTDNNDNAFSPNDTIRISAVYTNLLDPVSNLAV
                     TIQANTPDISFLNNSFLAGNMATMQSDSNRSNPFLAVIDPSIPKNTKVVFTLTFTSGA
                     YVDIQQFEVTVNVDYINVLVNDIGVTITSKGRIGFNDSGNSQGIGFTQNEGPNLLYSG
                     SLMIGVNDSVVSDAVAGTPAGTIDEHFTSLDYIHPIVPSVISEFDLTTNFDDQASSYP
                     IGVNVVHNTYVWSTPADRKYVIAEYVIANNSNNFYSDLYAGIYADWDITENTYATNRA
                     MFDSTLRMGYAFEVTANNNYTGIKLLTPGVAHFYAYNNDGSDGSINIYDGFSKQEKYQ
                     SMSGAGRAQAGMIGNGTDISMLLSSGPFSMLPYDSVKIAFALLGGDSLNEIVAAATAA
                     QLKYNSVGITEVATLENELLVYPNPAKSVLNVQVLNPNSGVAKIELYDVYGHLVAAKT
                     YLQTRTNTLNLDISTLSNGIYFMQYTSNQINKTIKINIQNDKP"
     gene            45693..46157
                     /locus_tag="PKX59_00200"
     CDS             45693..46157
                     /locus_tag="PKX59_00200"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HOZ89312.1"
                     /translation="MINPEKLQFLKNNFIELLKKLKGDEVPAWGKMNAQQMIEHFGDA
                     IEDACGKNNRTLITPPDKLEAVRAFILSDKDFKPNTKNSLMSDAPAPTQMPDLAAAIA
                     RVENGINEFEAFYSGNPEKKVMNPFFGELNYAENIQLLHKHALHHLRQFNLI"
     gene            46166..47314
                     /locus_tag="PKX59_00205"
     CDS             46166..47314
                     /locus_tag="PKX59_00205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ89313.1"
                     /translation="MSEGRKLISVIIVGPAYPFRGGIANLNEALCSHLNANGIKTEIV
                     SFTLQYPAVLFPGKTQMETSAQHFDFKITQLINSINPTSWIKTVGYIKNKKPDMVVFR
                     YWLPFMAPCLGFIARKLRKHGLPVVAIADNVIPHEKRIGDVSLTKYFIQSCDAFIVMS
                     DKVADDIKLFNPKKPVSITPHPIYSIFGDKVSRDEACEKLNLDKGFKYLLFFGFIRKY
                     KGLDILLQAMPLIKNKNLKLIVAGEFYESKEEYLKLIKEHHLENAIIMRDEYIPKDDV
                     RFYFSAADLIVQPYRSATQSGVTQIAYSFDKPMVVTNVGGLPEMVTDGVSGFVTDVNP
                     NAIANAVDRFYVEQLAIPFTAGVQSEKNKFGWDKMIATVKDIYQTLLK"
     gene            47355..49445
                     /locus_tag="PKX59_00210"
     CDS             47355..49445
                     /locus_tag="PKX59_00210"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HOZ89314.1"
                     /translation="MFLRRTFLICFLLFSYFANAQTERCGTQVYLEQWLAENPQLRKA
                     YDNEQAMAAARIENSNAMTATITIPVVFHVVYNTAEQNVPDDRIMEQLDVLNNDYNRL
                     NQDGQSTPSPFLGVSAAANIQFCLAKRDPNNKPTSGIIRKQTTVQSFSTGDEVKKTAT
                     GGSKGWSKSKYLNIWICNLTGNLLGYATMPGTANDTLDGVVVSYSTIGGPDNVGVHPT
                     YNLGRTLTHEVGHWLNLIHIWGDDGGTCTGTDDVADTPNQASEHFGTPNFPTISCSNN
                     PDGDMFMNFLDYTDDYAMNIFTAGQSLRMQDAVNNSRAALLTSKGCMDTTTAIDVALL
                     NVINVETIYCQGNITPSAVIKNKGAGTISSLTFSVKLDDNTATNKTWTGSLVAGATAT
                     VALDPLTVPTGTHYLNVSVTQSGDGNVSNNIIVKRITVTGATALPYSQDFEAKRFPAD
                     NWLVLNADNDATFMRSRLASINGSSSLWFYAPLQTPAGRKDIVQLEPFDLTTLAEPVL
                     AFDVACLNDNSISTSASLKILASTDCGSTFSEIFEKTGSALTTAEGSASNVFVPVAEQ
                     WRKDSVNLKALNSFNNVILRFEYTTGNANALFIDNLQIDRLGVIYPDASNVNVSVYGQ
                     SAYGVLDFSVLLLKTVDFKVDIFDVAGRKVFSKSYSGSAVCDRLSLHQLNAGVYFFRV
                     TSGKDSKSVKLFFR"
     gene            49655..50347
                     /locus_tag="PKX59_00215"
     CDS             49655..50347
                     /locus_tag="PKX59_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89315.1"
                     /translation="MKINSIVFIQIIILLLLFSCKKDKIEADSQITTNIDSTKTNIDS
                     TNNIPTTYDTIFPLDYFPAFPGSYWKYVDSNNDTTVRKTDSLYRKDYYTYGSAAYVSD
                     TFYVPIYNNIPIWKYEEHTGPISHSGSYPFRVILSETLPVGSDWPVFYWSGTGVSRKL
                     IAKDTTITISSIAYYPTIVIEEYYSAGPPHYIWIAKRYYTKNIGLIREDLYNNLDSTT
                     NTKELIDYFINI"
     gene            complement(50784..51665)
                     /gene="sucD"
                     /locus_tag="PKX59_00220"
     CDS             complement(50784..51665)
                     /gene="sucD"
                     /locus_tag="PKX59_00220"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOZ89316.1"
                     /translation="MSVLVNKDSKIIVQGFTGTEGTFHAGQMIEYGTKVVGGVTPGKG
                     GTTHLDRPVFNTVADAVKQTGADVSIIFVPPAFAADAIMEAAQAGIKLIVAITEGIPT
                     KDMIQVRDYLRDKEARLVGPNCPGVITAGECKVGIMPGFVFQKGRIGIVSKSGTLTYE
                     AADQVVKAGAGISTAIGIGGDPIIGTTTKEAVELFMNDPETDGIIMIGEIGGNLEAEA
                     ARWIKNNSKKPVVGFIAGQTAPPGRRMGHAGAIVGGADDTAAAKMKIMAECGIHVVAS
                     PAEIGETMVRVLSKVSA"
     gene            complement(51882..52694)
                     /locus_tag="PKX59_00225"
     CDS             complement(51882..52694)
                     /locus_tag="PKX59_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ89317.1"
                     /translation="MYNLLKGKRGIIFGALDENSIAWKTAQRAHQEGAMFTLTNAPVA
                     MRMGEINKLAAECNAQVIPADATSIEDLENLFTKSMEILGGKLDFVLHSIGMSPNVRK
                     GKHYTALDYEYFHKTLDVSALSLHKTLQTAMKLDALNEWSSVVALTYIAAQRVFPHYN
                     DMGDAKSLLESITRSFGYHYGMAKKVRVNTISQSPTATTAGSGIKGFNAFYNYADVMS
                     PLGNASADECANYTVMMFSDFTRKVTMQNLFHDGGFSFTGVSLNAMEKFMEP"
     gene            complement(52743..54653)
                     /locus_tag="PKX59_00230"
     CDS             complement(52743..54653)
                     /locus_tag="PKX59_00230"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOZ89318.1"
                     /translation="MPFKKSIYITTSAILIIVALLQWSFITVKENEYQQQALEAYLSK
                     SSLDFSGELEKLKILAGSNKVNSQQLIAQFKACRLAYKKLEGFTGYYFAYTERDLNGP
                     PIPMVEEEPQSKYVRPPHGLQVIEEIISEKPLNKAQLLDEINHTLGLAAKLATSFKTL
                     HPYDYQLFEIMQLRLVKIYTLGINNFDCQIIKTGIPETKAALFSISNILNISYRNADF
                     SMVNNSIDSAVAFLDRQQIRVNQGAEFDYFTFYRNYVVPVNLSLINARKNILKFDPQN
                     IRALNLDATTLFEKEAYNTYFFTKLQRSNNGDMLPYLGKLLFFDPILSVNNAVSCASC
                     HKPNMAYTDGLPKSYGFLKNDTLPRNTPTILNAALQRNLFMDASVLVLEDQAKTVVQN
                     EREMHGDFKVVVAKLNQSSTYKKIFSQAFAGTKDTAISEEGILKALAEFQRTQLSFDS
                     PFDKAMRGAGTIEQPVIDGYNIFMGKAKCGHCHFAGLFNGTTPPQYRESEFDIIGVPS
                     TNKRPYKLDGDLGRYNVFKNDELKYAFKTTTVRNIAVTAPYMHNGVYKTLQEVVDFYD
                     KGGGAGLGIHLPDQTLPDDKLKLTKDEKKKLILFLQSLTDTSAVVHFSGQLPAIDNSA
                     SPLNKRIAGGTD"
     gene            54875..56692
                     /locus_tag="PKX59_00235"
     CDS             54875..56692
                     /locus_tag="PKX59_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOZ89319.1"
                     /translation="MNYLSVENISKSFGDRVLFNNISFGIAKGQKLALVARNGTGKTS
                     LLRILAGLDVPDEGTVTFRNQLKVSYLTQENTLPQLATVADCLTENTHISDALLNEKP
                     HYELTEEEQTHLGKIKSVAGKLGVPFFHQKVNELSGGQQKRLALSKVLIDEADFIIMD
                     EPTNHLDLDMIEWLESFLASTNITMLLVTHDRYFLDEICDEILELEDGKLYRHKGNYS
                     YYLEKKAERDEMESANLQRSKNLFRTELDWMRKQPRARGTKSKSRIDAFHVLEEKTKK
                     KNEQGNVALDINISRIGSKILEIHHISKSYGDLKLIDNFDYSLRKGEKIGIVGSNGAG
                     KSTLLNIIMGKEPCDKGKIVMGETIVPGYFEQQSKNISDQKRVIEVIREIADFIPMSG
                     GRKISASQLLERFLFDPSMHFQYVEKLSGGEKRRLYLLTVLMKNPNLLILDEPTNDLD
                     IQTLSVLEDYLASFEGCVIIVTHDRFFMDRVADHIWAFEGDGKISVLNGNYYDYRQML
                     KEQKREQKKEAAAVVRTKPEETTANKKRKLSFKEKQEYEKLENDIMLLEEEKTMLEQQ
                     MGEAELTHEKLMEVSNRFQQVLHDIDAKTMRWMELAEIG"
     gene            complement(56700..57734)
                     /locus_tag="PKX59_00240"
     CDS             complement(56700..57734)
                     /locus_tag="PKX59_00240"
                     /inference="COORDINATES: protein motif:HMM:NF025100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HOZ89320.1"
                     /translation="MGIYIKSSVCISARNTFGETDLSEHSLQTEKLMAREPVYSMIAP
                     AVLRRMGKAVRMTCGAVSKLNLENHPVNGIIIGTANGGMEDCIRFLNQIVQYDEGTLT
                     PTNFVQSTPNAIASTIGMNHQCRGYNITHVHRGNAFENALLDAFMYLVENKNSTLLLG
                     GIDEISDYNYNIERLGGWYKNEIPVNGLYESKTEGSIAGEGCAAFTVSNNSESALLEI
                     VDVSTLHSTDVDEVLQQLKQFCDKHALNLSETMLVSGHNGDVRNETLHYAVESHFQQS
                     AIIRYKHFFGEFPTVSALALWLIGQAATNSGILPEHFYLRKSESKIKNYLICNHYKGY
                     QHGFILTKTP"
     gene            57917..58651
                     /gene="gldF"
                     /locus_tag="PKX59_00245"
     CDS             57917..58651
                     /gene="gldF"
                     /locus_tag="PKX59_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HOZ89321.1"
                     /translation="MYSLFKKEINSFLNSLIGYISIAVFLIVIGLFLWVFPGNDLNIA
                     DSGYAAVDSLFNITPWVYMFLIPAITMRMFSEEKRTGTLEILLTKPLTEFQIVLAKYL
                     SGLVLVIFSLLPTLVYVVTVYKYAAPVGNIDLGGIAGSYIGLFFLASGFVAIGVFASS
                     LADNQLVAFIIAMFLCLLCYTGFQSVATILATGVVANLIDQLGIVAHYNSMSRGVIDS
                     RDVIYFISLTALFLVFTRLKLESRKW"
     gene            58653..60338
                     /gene="gldG"
                     /locus_tag="PKX59_00250"
     CDS             58653..60338
                     /gene="gldG"
                     /locus_tag="PKX59_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HOZ89322.1"
                     /translation="MKKKIRSANLYQFLLLLLVIAGLNAAGKFLFWRFDLTTEKRFTL
                     SPTTKEKLKQLKDIVYVKVYLTGDLPAGFQRLSSATRDMLMEMKSYAGSNLEFDFIDP
                     SALADSKQRNELFNQLADKGLQPTNISERNAEGTSERILFPGAILNYLSKEQPLLLLK
                     DRMGASAEEMVNTSIQNLEYEIINAISKISTRKPATIGILHGQGELDKPYLADFYKSV
                     SASYPTHYVQIKNQLNALQDYDCLVIAKPDSAFDEKDKFIIDQFIMRGGKVLWLLDMM
                     SADMDSLARKNEFIAIPKELNLDDMLFRYGVRLNKDLVMDLQSVPIPILTGYVGNRPQ
                     NSLLPWFYYPLVTPTSKHPIVNNLNAIRFAFAGSIDTIVSAHIKKTILLQSSHYCKTV
                     QSPAVVDLNILRKDPDEKEYRNPPVNLAVLLEGSFRSNFANRIPYQIATDSTIGFKAD
                     GKPTSMIVISDGDVIRNDYKRSSNTVYPLGMDRYTGQFYGNKNLLLNCIDYLCDNSGL
                     MALRAKEFKLRLLDKTRFDPDKKMLQIVNVALPVLLIILFAMIKLLMRKKKFS"
     gene            60346..61371
                     /locus_tag="PKX59_00255"
     CDS             60346..61371
                     /locus_tag="PKX59_00255"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOZ89323.1"
                     /translation="MNKNRILLLIVVLLLSVTGYYYFNNSDSTIKKELRDFAVADTSS
                     IDKMFLADKKGNKALLERKGPALWTINGKYPARQDAINFLLKTIKQIEVRSPVGKAAL
                     QNVLKELSSTGTKIEIYSKGDKLKTYYVGGPTQDMLGTFMYLENSTVPFVIHIPGFNG
                     YLTPRYFASADEWRSRIIFQYGEGQIASVMVQNNQHPDESFRIKKDDDHYVYYSSLTA
                     TAPAEIFQSQLVAYLAKYQLIGFERPTYDLDVAYRDSLLKTGPVRILEVEGVDGKKDK
                     IEMYLKPVDTGTMTSVDERTGLLREFDPDRMYASWNNDTNLIVVQYHVFDKLFSKPDR
                     IKGFGSL"
     gene            61438..62559
                     /gene="dnaN"
                     /locus_tag="PKX59_00260"
     CDS             61438..62559
                     /gene="dnaN"
                     /locus_tag="PKX59_00260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOZ89324.1"
                     /translation="MKLIVSSLVLQKHLQSISGVLTTNSPLPILDNFLFAVNGSELTL
                     SASDLETTITTRLKVESKDKGIIAIPGKILLDILKTFNEQPLTFTINEKNHTIEISSD
                     YGKYKLNGQNGEDFPRIPVLEKSNHIELPSSVLHEAINKTIFATGNDDLRPVMSGVFC
                     QLSQDSTIFVATDAHRLVRYRRTDIKSKEAGSFILPKKPLNLLKNILPQEETRVKVDY
                     NEANAFFSFDNYDLICRLIDGRYPNYEAVIPVDNPNVLTVDRVAFLNSIKRVSIFSNK
                     TTYQVKLAIKGSELAISAEDVDYANEANERLTCSYDGSDMDIAFNARFLADIVSNLDT
                     EEIHLHMSLSNRAGLLTPGKDHTNAAEDILMLIMPVMLG"
     gene            62624..63349
                     /locus_tag="PKX59_00265"
     CDS             62624..63349
                     /locus_tag="PKX59_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOZ89325.1"
                     /translation="METRQQKYAERYKDVIGASFSFETAQPELPLYYFVSGFAHPFLP
                     VIKHDGLFLFEWGLIPSWIKDAKGASEIRTKTLNAVGETVFEKPSFRKSIVSNRCLLG
                     VSGFFEWRDVNKKKYPYFIKTNNSELFSLGCIYETWVDKNTGELHNTFSIITTVANPL
                     MEKIHNVKKRMPLIIAEEDERKWVDPSTGIDEIRSLIKPYNESEMTAYTISQAANSAR
                     ANRNVPEILNAVNYPELALLDTI"
     gene            complement(63352..64101)
                     /locus_tag="PKX59_00270"
     CDS             complement(63352..64101)
                     /locus_tag="PKX59_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOZ89326.1"
                     /translation="MKNIKAIVVDDEMFGREQIKLLITKYFPQITLVAEVANAAEALK
                     QIYFHKPDLIFLDVEMPDSNGFDLLEKIQEHDFEIIFVTAFDQYAIKAIKFSALDYLL
                     KPLNKEEFKSAVQKFLDRQKSEYDRKSVINNFIRNIKSEEDKLFRLAVNTSNKTYFLL
                     PDEIIRCEADGNYTKITCGNKEVVLSSKTLKEFDDLLNSYNFIRVHRAHLVNKKYIRS
                     FENENKLVLSDNSAIEVSRRKIAEVRNLFGQ"
     gene            complement(64134..67022)
                     /locus_tag="PKX59_00275"
     CDS             complement(64134..67022)
                     /locus_tag="PKX59_00275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ89327.1"
                     /translation="MMRFVIFILLTFYPLISVYAQDASRIPVFLNNLQTATTDSARVE
                     AYRDLCFNYSIANTDSGIYYGKKGLEIAQRIKFLKGIGDSYNSLGWCYSRKGDYTKAK
                     KHLTLALQYFEQTNDKCNVAVVLSNIGSTYFNQSLYADALEYYLKSVDLSNACTDAIR
                     KPAGLYSIGIVYNSQGEYNKAIPYFREAAALNEQINDSNKLADCINGIGNAYLGLEMY
                     DSAFHYLNQSSAMYQKLSNISGTAYAAESIGSIYLKQGNFDMALKQFEFARENFEKSG
                     SQNDLCYELTLIGDAYRKYGKINSAIIYMKMALQIADSNRFQTLQLQTLNSLSELYAM
                     KHDYQNAYLTFKQAAEIKDTINETKQQARLDEIKTKFETEQKDKMIEILNRDKAIEKS
                     NAEWQRQLKNIFIVGAVMLFLILLNLYNRFSLKKRAAQQLEQKNIIIAQEKERAERSE
                     KFKQQFLANMSHEIRTPLNAIFGMTELLQETKQDEQSSKYLQVIKQSSESLLVIINDI
                     LDLSKIEAGKMEIEKTPFRFRETINHVYQTFSHKAASKELDLKFSVGENVPELIIADP
                     FRITQVLINLVGNAIKFTERGFVIVSINQVRASQKHGLNYCDLLFEVSDSGIGIPAGQ
                     QEKIFESFQQTYAGNSRKYGGTGLGLTISQSLVQLMGGNIEVSSVENQGTIFSFTIEV
                     EVADALAETNKSEQVPIQLIIDKDAFSVLLIEDNEFNQILVKDALSKKMRDIKLQIVG
                     NGRDAIEILKNQKFNLILMDISMPEMDGYEITQIIRNELKESVGEIPIVALTANVSQH
                     EAEKCIAAGMNDYLSKPFKISELTDVINRFKGDVDEQKIDSRIKVAPIASSVDLAYLR
                     DFTDGDIQEMLRYVDMYLQRIPEVFNEIEISLKGDEEKHVIVLLHSIKPLIASVGLND
                     CVQKISELEKQRETIKKNKLMPVVLLIRRQCMTMVDSLKLWKVNNSAG"
     gene            67199..68839
                     /locus_tag="PKX59_00280"
     CDS             67199..68839
                     /locus_tag="PKX59_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89328.1"
                     /translation="MKLLYTLALSLFTAHLTFAQFSSDPSQPMPVCNAAHGQSNVISL
                     PDGNDGNYLFWLDNRGSVNTTIYALYGQHLDSGGNPLWANNGKVILDKDTVSIKSFNC
                     VLFDGGILVTWVQGQNSYNDSMFVKKINPSGNDVWAQPTLIESTNFSASILGIETYGI
                     KAIPNDSGAFINYSVVYIGGSSGIKFNRINFNGVKQWAASKTNSLPGYTFFTYPGIQN
                     TFYSLSRGNGMGSGIYIQKYNMQSVPQWANPVDISQGVSGNGLAGNCKFLTDNSGNSY
                     VVWDSYSSADILLTKIDAAGNFAWTPSYKSVVNLANTLQGRSDAVLNNNNLFITWSDN
                     RITGQATAFTQKYNASGAAQWIANGVQLGVINSNYCYPKIAYSDSGSVAIAYIMGAPW
                     ELYVQRVRSNGTLTWSGSGKILASDHSFINYADYTITSDSNGCNAVFWHESSTGDLYG
                     AKVCSDGTLVTVAELLNKEVKIFPNPTADQINIELGSSNLINEIEILDMQGRILKTVI
                     IENGQQYQTLNVNEFSKGVYLIKIKAGNESIIKKVIIK"
     gene            68900..69034
                     /locus_tag="PKX59_00285"
     CDS             68900..69034
                     /locus_tag="PKX59_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89329.1"
                     /translation="MSKQPPKINHEIIRERALDRILFILEIALVITLIALCGVIITSL
                     "
     gene            69501..70052
                     /locus_tag="PKX59_00290"
     CDS             69501..70052
                     /locus_tag="PKX59_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89330.1"
                     /translation="MQQHSIMLFFMLLVGIGTIFFVSIFMSSLKADNDSTGVKNLLKK
                     RFWFTLILIVVLGIFAAATLPKSPYFTYANETPSTVIHVGTMQFSFFLSTKPIDPKSP
                     SGETIEIPHDQLVEFRVTSFDVNHGFAVYDPSMRLITQVQAMPGYVNNLRYKFTEPGE
                     YHIFCLEFCGTGHPVMQTTFKVI"
     gene            70068..71519
                     /locus_tag="PKX59_00295"
     CDS             70068..71519
                     /locus_tag="PKX59_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOZ89331.1"
                     /translation="MNIDSLKLKKISALWIIVILVLFIASIVLGILMRLNQGKVIEQS
                     PLTFYTNMTTHGLTMIGIWFAAGMVAVNYLLQRYVKTSYGVNLFAFILTVIGVVMLWV
                     TTYAGKFSTGWTFLYPLPFRDLGSESWSTPLFLISLTILGVGWLVWSVSLMAQIFKKY
                     TLAQAFAWQHFKSNPTVETPPFILITTVTLVGVIASLIAAVIVIVLYFAEYFSHGDFV
                     NDALLMKNLIYFFGHTIANEMLYLGLAVIYEIFGEVSDRPKWKMTWYISLAWNCAMVF
                     ILTAFFHHLYMDFVQPQALQIVGQLASYLASIPSAAVTVFSIVAAVYGVKVRWSIVNL
                     LFFLGVAGWVIGGLGAVIDATISNNIMLHNTLWVPAHFHTYNAMGNVLFSIGFFYWFS
                     TQYVDKLVLSKLTKYTLTLLIVGGIGFLMAFYVAGANSIPRRYSTYPVELTSGPYLAK
                     VGALFATIYLIAILIFFYNIAKRCFVILSASSR"
     gene            71486..72238
                     /locus_tag="PKX59_00300"
     CDS             71486..72238
                     /locus_tag="PKX59_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOZ89332.1"
                     /translation="MFRYFVCFFALTCFSFLGYANDNHSDFREEQNLYGKIYNAPLGI
                     VGNNKVSSVEELYNQKPLIIALVFTRCYGICNPFLLQLSENIKHLQSNSDFNILVISF
                     DPRDNLQDMTNLSKQYGLENNLQWNFAITDSIDAFINSIGFIPKWDSVIKQFDHDALL
                     VGVNEKGLITKNLLGIRIVEDVHKMISSINNVFTPSYRLPTKSNIFSCFNYDPKTQTN
                     RPGLGLLFVALPAIITFLIVTIIVIKSKRTVS"
     gene            72344..72697
                     /locus_tag="PKX59_00305"
     CDS             72344..72697
                     /locus_tag="PKX59_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOZ89333.1"
                     /translation="MSSVKIKRIYEAPDKSDGYRMLIDRLWPRGVKKENAAIDEWNKD
                     IASSSELREWFGHDPEKFKEFTARYTKELHNKNDELKQIAGIAKKQNLTLLYGAKDTE
                     HNQAVVLQKVLSKMI"
     gene            72694..73161
                     /locus_tag="PKX59_00310"
     CDS             72694..73161
                     /locus_tag="PKX59_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOZ89334.1"
                     /translation="MSTKPIKRHEAIQSISREHYQGLLFGSKIRMGLKKQIEPDRMAK
                     YAVWFYKEHLIPHFEIEEKYLFPVMSPENEMIQRVLKEHRMLADLCKSELELATNSKL
                     ELLEKTLEAHIRFEERVLFKEIESIATSEQLELINKHHKEEKFVDNLSDPFWK"
     gene            73189..73620
                     /locus_tag="PKX59_00315"
     CDS             73189..73620
                     /locus_tag="PKX59_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013410266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOZ89335.1"
                     /translation="MLHSNIQTENILVARNTRPTSMRILVYNFLAKQQAALSITEIEK
                     HFQHADRITIYRTLKTFEEKGIIHSIQEDNITKYTLCGSACSEHTHYDQHLHFYCKSC
                     KQTTCKTDIAIPLNNITGFKIDDITFFARGICEKCINPTLQ"
     gene            73668..75668
                     /locus_tag="PKX59_00320"
     CDS             73668..75668
                     /locus_tag="PKX59_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOZ89336.1"
                     /translation="MTHKHIYDSSGKQLCCTLEDNIYENAGRVDLKENHDHNDHDHTH
                     HESGMFSIYLPGLVSLVLLLAGMHFDYWNTFSWFNGWIRLVWYIIAYIPVGFPVIKEA
                     YIGITKGNIFSEFFLMTIATVGAFAIKEYPEGVAVMLFYAVGELLQTMAVRRAKSNIK
                     TLLDQRPDVATVLRNSNTVSVDANDVGIGEVILLKPGEKLALDGILLSDNGTFNTAAL
                     TGESMPDAKVKGASVLAGMINLNTVVKIKVTAAYEDSKLSKMLELIQNASAQKAPTEL
                     FIRKFAKVYTPIVVGLAVAITLLPYFFAHEYIFRDWFYRALIFLVISCPCALVISIPL
                     GYFGGIGAAGRNGILVKGSNYLDVLASVQHVVMDKTGTITEGVFKVQEALIFPEENRN
                     KILQMVNALEKHSTHPVATAIHNYVSNTSTIELEKVEEISGHGMKAFVNGKELLVGNF
                     KLLSLHNISFNVDPATIKHTVIAIAFDRKFAGYIIIADSIKTDAAAAIQKLKQMHVDV
                     TMLSGDKTTVVNSVAEQTAISHAYGELLPEDKVEKVKSIINQKQTVAFVGDGVNDAPV
                     IALSHVGIAMGGLGSDTTIETADVVIQDDNPIKIPLAINIGKQTKKVVWQNITMAFVI
                     KAIVLILGSGGLATMWEAVFADVGVALLAIINAMRLLKMRFD"
     gene            complement(75731..76690)
                     /locus_tag="PKX59_00325"
     CDS             complement(75731..76690)
                     /locus_tag="PKX59_00325"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOZ89337.1"
                     /translation="MSNYLDFEKPIEELYEQLEKTKQIGNKGKIDVNNLLTEIEEKIQ
                     VAKKQIYSNLTPWQRVQLSRHPDRPYSLDYINAITDNTFIELHGDRNVKDDKAMIGGF
                     GTVDGRTIMFVGQQKGHNTKSRQYRNFGMPNPEGYRKALRLFKLAEKFNKPIVTFIDT
                     PGAYPGLEAEERGQGEAIARNLYEMSILKVPVICIIIGEGASGGALGIGVGDKVMMLE
                     NTWYSVISPESCSSILWRNWDNKEKAANALKLTANDMLDLKLIDGIIKEPAGGAHSNP
                     EEIFATVKKEINVQLGALLTVSDADRVNNRIDKFCSMGVVRKN"
     gene            76784..77608
                     /gene="ispE"
                     /locus_tag="PKX59_00330"
     CDS             76784..77608
                     /gene="ispE"
                     /locus_tag="PKX59_00330"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOZ89338.1"
                     /translation="MLNFPNAKINLGLFITSRFDDGYHALETLFYPINWCDILEITEN
                     KNYSKSQPKVNLYISGLAVDGPTDQNLCSKAYHLLKEKFNLPPVNIFLHKQIPTGAGL
                     GGGSSDGAHTLLLLNKLFDLKLSNEDLSVFALNLGSDCPFFISNKPSFAFSKGEILKP
                     APTVLEGNFIVVIKPSVSVSTAMAYSQVKPDKPNKSLTEILNLPKIQWKDFLKNDFEK
                     TILPQFPQIEKLKTNLYQAGAYYASMSGSGSAVFGLFHDEVKLSFLKDEQHHSAWL"
     gene            complement(77609..78346)
                     /locus_tag="PKX59_00335"
     CDS             complement(77609..78346)
                     /locus_tag="PKX59_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89339.1"
                     /translation="MQLRLTVIFFLLSFGVIAQDVRYDYVEDLPLRYTNEYSIGATLH
                     SSGWGFMFRKGKNLTVSRKRMYEIEIVSMKHPKEYKSVSYLSERPKTFVFGKMNSLLV
                     MHGGFCYERVLFDKAERRHFEIRWNSTGGPSLGFTKPVYLIFATGNGQRVVEKYDPNN
                     QNHDRQSIYGKAEFTKGLDELKLYPGLFLKSGVMFEYNIMNDGITAVEVGVMLDAYTK
                     TIPIMAFAKNKQVFFNLYGTFMLGRKK"
     gene            78451..78744
                     /locus_tag="PKX59_00340"
     CDS             78451..78744
                     /locus_tag="PKX59_00340"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOZ89340.1"
                     /translation="MFRKTNEESLKEVIEQLLETYKLRDKLNQVKLQRSWDEIMGEMI
                     SKRTEKLILKDHTLYIYLNSAPLKEELSYGREKIVQMLNTALEGDFIKEVVIR"
     gene            complement(78808..78880)
                     /locus_tag="PKX59_00345"
     tRNA            complement(78808..78880)
                     /locus_tag="PKX59_00345"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78845..78847),aa:Lys,seq:ttt)
     gene            complement(78970..80133)
                     /locus_tag="PKX59_00350"
     CDS             complement(78970..80133)
                     /locus_tag="PKX59_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89341.1"
                     /translation="MQVIEVNTAAHKKDFLLLPKQIYASDANWIQPLDNDIESVFDAT
                     KNKFFRHGECTRWILRNSSNKVVGRIAAFIDKKTATKNNKQPTGGMGFFECIDDKEVA
                     FKLFDTAKEWLISRGMEAMDGPINFGERDSWWGLLTDGFHEPPYKMNYNPTYYQEFFE
                     SYGFEIYFKQYCYGLKVRNQLDEKFYERHALIKANKDFKAVHIKKSQLEKFAEDFRTI
                     YNKAWVKHGSGKELESKQVQQFFKKMKPVIDEKIIWYVYYKNEPVAFWVNLPDINQIF
                     KYFNGKFGWFEKLRFMLMLKRKVSKRMIGLVFGVIPEFQGKGIDSFMIVDGTAHIMNE
                     TKYEDCELQWIGDFNPKMVSVAESLGSWRSRTLITYRYLFDRNKPFERHPIFA"
     gene            80185..80991
                     /locus_tag="PKX59_00355"
     CDS             80185..80991
                     /locus_tag="PKX59_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ89342.1"
                     /translation="MKNKVVIITGATSGIGNACARYFAQQGCHIVIAARNEEKLNRIG
                     NELKSLYGNCLSIKTDVALEDDCKNLIQKTIDTYNRIDILINNAGISMRALLNDVSLN
                     VMHQVMDINFWGTVYCTKYALPYLLKSQGSVVGVSSIAGKIGLPARCAYSASKFAMEG
                     FLESLRIENLKNNLHVLVAAPGYTASNIRNTALNKDGQQQEESPLDESKLMKPETVAY
                     EIYNAVLHRKRDLVLTTNGKLTVWLNKFFPSWLDKIVYNFVAKEPDSPFK"
     gene            81002..81532
                     /locus_tag="PKX59_00360"
     CDS             81002..81532
                     /locus_tag="PKX59_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ89343.1"
                     /translation="MSIRTIIFSALSCILLASCTNTKQQLKDEIDSKEKLLYENKNGR
                     INTKLAREVIIHYRYYAEKFPDDSLSVEYLFKAGDVSVGIAAYDNALEYFKIITEKYP
                     KSNRAAYSLFLQGYISENNLEDTLNARKYYNEFLEKYPNNEMAESARFSILNLGKSAE
                     DVMKQIDGNKDSISEK"
     gene            81535..83478
                     /locus_tag="PKX59_00365"
     CDS             81535..83478
                     /locus_tag="PKX59_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ89344.1"
                     /translation="MTKNKKQKESIAEAHQKKQQQKADEARNHKIKILRRILALICGL
                     CGVILYLNTLHHGFVLDDFSVILENRITKQGLKAIPQIFSTSYRSGYYLGTDELYRPI
                     PKSIFAISWNWFGNNPLPLHLLNIVLFGITGIVLFNFCYLLFPNLVFSFIASMLFMFH
                     PVHTEVVANIKSVDEILSFLLCISACIFLARYVQTKRISELVLTSVCFFAALLSKESS
                     ITFLAVFPLLILLKSDKKETSYNWSVELKPMLTLTAFALLFLMIRHSVLSKIASGDPS
                     IADNLLMAAKSSDQKFATAVMILGMYLKLLFIPHPLVFDYSYNQIPLVGMGNIWFLLS
                     LLIHVGLLVWAILLLKKNKLLSFIILFYFISMSIYSNIFYVIGSSFGERFLYTASFGF
                     CMAVAYAFSLLSKNQNVETVSSFFKNNLTVFLIFLPVAFAFGYKTITRNTVWKSNYTL
                     YANDVNLSPNSTRTHYYYGNLLAKDDFQKDKTDQEKDSIQKLALSELEKSVAIFPFGD
                     AYNQMGIIYDKRKEFKKSFESYQQAMKAAPNDPMIQNNLGSLYFNMGQIPEAKACYEK
                     AVQLNPSYSEALMNLGSACGMMKQYDDAVTNYLKAIKFDPNLAQAYYYLGLTYRFKGD
                     QQNSEFYYNQAAKMDPEKYGKKK"
     gene            complement(83527..84234)
                     /locus_tag="PKX59_00370"
     CDS             complement(83527..84234)
                     /locus_tag="PKX59_00370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOZ89345.1"
                     /translation="MNKFNVTVFSFLAMAFSACGQKNTGGSQAITTTLDSVSYGIGVS
                     IGENMKKDGLTDMNVDLISRGISEALKGEKTALTMEQVQACIGAYMGEKQKVKDQEAK
                     AKAGVNLEKGKKFLEENGKRKGVVTLPSGLQYEIIKEGTGPKPAATDKVTTHYHGTTI
                     DGKVFDSSVERGQPAQFGVNQVIPGWTEALQLMPVGSKWKLFIPANLAYGESGAGGAI
                     GPNETLVFEVELISIDK"
     gene            84456..85136
                     /locus_tag="PKX59_00375"
     CDS             84456..85136
                     /locus_tag="PKX59_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOZ89346.1"
                     /translation="MKITYYGHSCFLLEVASKRILFDPFITGNPLAVSIKIQSIKADY
                     ILISHGHGDHIGDAVEIARQTNALVISNFEICNWIQDKGINQVHSMNIGGHKLFDFGK
                     LKCVNAIHSSSFPDGSYGGNPMGFLLETAEGNLYYSGDTALTYDMKLIGDYKRIDLCM
                     LPIGNNFTMGIDNALIASEFVKCDRIIGMHYDTFEPIKIDHAEAKDKFSNAGKELILM
                     NIGSTMTF"
     gene            85166..85999
                     /locus_tag="PKX59_00380"
     CDS             85166..85999
                     /locus_tag="PKX59_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ89347.1"
                     /translation="MAKVISIANQKGGVGKTTSAINLAASLAVLEYKTLLIDADPQAN
                     STSGVGFDPRKITNSIYECIINDLDPAKAIQETQTPNLFLLPAHIDLVGAEIEMINLS
                     NREYMMKQVIDKISADYEFIIIDCSPSLGLVTINALTASDSVIIPVQCEYFALEGLGK
                     LLNTINIVQSRLNPNLGIEGILLTMYDSRLRLSNQVVEEVKTHFQNMVFSTIIQRNTK
                     LGEAPSFGETIIMHDASSKGAINYLNLAREILQKNNLTGVPTQREKLFKAEETKKVRM
                     N"
     gene            86065..86955
                     /locus_tag="PKX59_00385"
     CDS             86065..86955
                     /locus_tag="PKX59_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOZ89348.1"
                     /translation="MSQKKSGLGKGLSALLDNSISVNEFKEIGVATSNAQITTLPVDR
                     IEANPFQPRSEFKEEELLNLADSIRVHGLIQPITVRKLSADKYQIISGERRFRAAQLV
                     GLTNVPVFIRLANDQSMLEMALVENIQRENLNAIEIAISYKRLIDECKLTQEDLGLRV
                     GKDRTTVTNYLRLLKLPPQIQTALRDEKISMGHARAIISISDPVMQLKIFNEILENDL
                     SVRSVEDIVRGSSKNEKKQPSISEKFIKGIEYKHIQKRLEDKFETKIEVKHRKNGSGQ
                     IMFTYFSTDDLNRLLDILDA"
     gene            86968..87564
                     /locus_tag="PKX59_00390"
     CDS             86968..87564
                     /locus_tag="PKX59_00390"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOZ89349.1"
                     /translation="MRLRLLVVLFFTVYCVNAQQDTMIFKTRTNVIVKADSIKPTSDL
                     SQKVNKHSPHKATIYSAILPGLGQAYNKKYWKIPVIYGLTGVLTYFAIDNNKEYVVYK
                     DAYKWRLDDNSATVDKFEGIYSDEDLRILKNYYRRNRDLSFIGMGVVYLLNVVDAAVD
                     AHLFYFDVSDDLSLKVQPGIQPMSHTAFAGVKLSLQIK"
     gene            87568..88281
                     /gene="dapB"
                     /locus_tag="PKX59_00395"
     CDS             87568..88281
                     /gene="dapB"
                     /locus_tag="PKX59_00395"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOZ89350.1"
                     /translation="MKIALLGYGKMGKEIETAAIESGHQIVLKINSANYNQLNKESLQ
                     TADVAIEFSKPEAAIANAKLCVDSNVPVIIGTTGWYEHKATLEQYCIQNNGTVIYASN
                     FSTGVNVFFIINEMLSKLMAPMKYDAWIEEVHHIHKKDIPSGTAITLANDVLKYNKQY
                     SGWAINDEDGKLPIYAQRIDEVTGFHSVSFRNEVDTIEITHNAFSRKGFALGAIKAAE
                     IALTKKGFYEYKNLLIEQP"
     gene            88286..88927
                     /gene="nth"
                     /locus_tag="PKX59_00400"
     CDS             88286..88927
                     /gene="nth"
                     /locus_tag="PKX59_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOZ89351.1"
                     /translation="MLLKERYKYVISYFRKNMPVAETELVYKNPYQLLVAVILSAQCT
                     DKRVNMVTPELFKAFPTAKKLSETEPEEVFEYIKSISYPNNKAKHLVGMARMLLEKFE
                     GIVPSELDDLIQLPGVGRKTANVISSVVFNKAAMAVDTHVFRVAARLGLTRNAKTPLA
                     TEKQLVKHIPEELIATAHHWLILHGRYVCTARNPKCNICGLKDVCVYYKKHPV"
     gene            complement(88924..89538)
                     /locus_tag="PKX59_00405"
     CDS             complement(88924..89538)
                     /locus_tag="PKX59_00405"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOZ89352.1"
                     /translation="MLIEINENNIDDRKIKKVVECINNDGIIIIPTDTVYALACSIYS
                     LPAIEKVSRFKGVKPEKANLSFLCNDLKNIAEYTKPFDRSIYKLLNRSLPGPFTFILE
                     ASTKVPGIFRARKKNIGIRIPDQKVTLSIIQQLGHPLMSASLHDVNDPITDYLTDPDE
                     IYERFSDMVDMIVDCGAGGNVPSTIVDCTTDEITVVREGKGVLS"
     gene            complement(89580..90638)
                     /locus_tag="PKX59_00410"
     CDS             complement(89580..90638)
                     /locus_tag="PKX59_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89353.1"
                     /translation="MKRFKYLLILLPFSLNFALAQDVDTIENKHLINIKQLTFGGDNA
                     EAYFSFDNNKLAFQSNNKAWGVECDQIFLLDLNNWKSDSKPPLISTGKGRTTCSYFMP
                     GNKTILYASTHLRGDKCPETGDLRRGGKYLWPIFNTYDIFVADLNGKIVKQLTNAPGY
                     DAEATVSPDGKKIVFTSDRTGDLELWTMDIDGSNQKQITFGLGYDGGAFFSPDSKQLV
                     FRSSRPTTAEEIQEYKQLLSEGLVAPTNMEIYTCNVDGSNLKQITHLGRANWAPFFHP
                     SGKKILFSSNHESKSGFNFQLYMINTDGTGLVQITDQSVFNAFPMFSPDGKKLIFSSN
                     RNNGGTHDTNLFIADWKD"
     gene            90820..92481
                     /locus_tag="PKX59_00415"
     CDS             90820..92481
                     /locus_tag="PKX59_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89354.1"
                     /translation="MKKLFTVLTILLIASTNQAQNVGIGTNVPMQKLDVIGKIRSSTL
                     TGVGNRFVMADPTGTLNVSSSGLLDSTAWLTLGNSSIDTTKNFLGTLTNTHILFKTNG
                     NAAANERMRIRSGGEIIVNNTSVGNNNGDVFSVYATGSSNGINNALSPIGAFALNGYS
                     ATTGIGVSGANTGTGVGVWGSSSSGYGTVGFSGGNTAFAGMFQNSNANGTGLLVTGNN
                     LPGTYLVNGSAGSFRGTNFGIINFSNISSAAVAGSAVWGSNRKTIITSYLGGAGVTGI
                     DSAYGSGVIGVSFFGGNNGVTGIGSSNSGTGVMGQAGMGPTPYAVWGDVTTVSGTNAT
                     SIAVIGSNNSVGANAIGILGQEVAAPNGPTRYAIFANGDLAASGTKTFVIDHPLDPSG
                     KFLKHFSIESNEVLNYYRGNVVLDANGEATIQLPSYFNSINNSNYNYNLTPIGAPAQL
                     YVKSEVTGNNFVIAGGVAGMKVSWTLTAERNDPYLQQNPDSRLVELPKTGDAAGKYLV
                     PSLYGQPESAGIYYKPIPQKTAVNEKSKDAPLIKSRDFTQQNDSK"
     gene            92515..96540
                     /locus_tag="PKX59_00420"
     CDS             92515..96540
                     /locus_tag="PKX59_00420"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ89355.1"
                     /translation="MKKFVYYILLFLLPVTAWSQCMMTPVSLQDRISNATVVVEATAL
                     QQNSYWNNSNDFIYTSTTLSISAVYKGTIQGSQIALITEGGIVGNIMIKSEPSLNIKP
                     GQNGIFFLIPHSQPSGSKLSGMFEGYALSQSLILFDETSNSATGPFENYSDINALRNQ
                     IINATGGVQRISSIAPQQAPPSVNKIMIPPTIASIVPATTTAGTFSTITINGNNFGAT
                     YVNGTSNVEFPDANTGGVSYISAPQNHIQSWSNTQIQVWVPTQGGSGLIRVTNNLGET
                     TTSAISITINYNETNVVSGSNYYQPDLINNNGTGGYTFRYNTTFNGNAAAVAAFERAL
                     QTWRCGTFVNFNRSGTTATTCQALDGTNLVTFDGSCALPAGVLGISYSYYSACATGVW
                     YLNENDLKFRTNGTGGINWNYGPAATGGGNFDFESVCLHELGHSHQLGHTIVPVTVMN
                     YAIGPNTDRRTLTPISETAGGNDIMSRSVVTNACGPTAMVALSAGSCAINAPIADFNG
                     TPLTGCNTLNVTFTDASINTPTSWTWSFPGGVPNTFVGQNPPVIFYAAPGNYAVTLTV
                     SNAFGSNTLTKTNYVQIATCPPPTPDFTANPTTVCQGNTVQFVDLSTNTPTSWSWTFP
                     GGTPGTSTAQNPVITYATAGTYNVTLTSTNAYGSNTLTKTNYITVNNCPAPPVADFSA
                     SPTTLCSGGTVNFTDLSTNTPTSWSWTFAGGTPLSSVAQNPTITYNTPGTYAVTLIAT
                     NGGGSNTKTVSGYITVNLCSAPVASFSGFPTTICEGQAVTYVDQSTNSPSSWAWTFTG
                     GAPAASALQNPVISYATAGTYTVSLTATNAFGNNTFTRTNYINVVTCPSPGTGLIVND
                     GSLIFVQPTAMMHVEGGVINQDNLSNIGTWDNSGQVRIQGDWTNNSSGNAFINSSPGE
                     VELFGANQTIRGSTPTFFFTLNLTGTGIKTQNIDATVQGLLKLNDRELATQGNVMHVT
                     NTAPAAITRTGGLNSTPVQGFVSSTGSGKLWRNTNTTSVYLFPVGSSIPPARFRPVEI
                     KPLSATPNTFAVRMVNNDPNTDGFNRAVREPTLGAVNAGWYQKINRVSGSSLAEFSLY
                     ADDAQDAIASWPLLKLCQWGQAAPPVWWKDIGGAVTPGASPVLGSVLRTNWNNFSNTE
                     NFNIAPESNPLPVELLSFTGNCKNNIVNLNWTTASEINNHYFELQQSADGNDFVTIGR
                     VEGNGTTIKQNFYSFTIDQKTEHTFFRLVQYDFDGRANVLPAILVSCNTESPATVNIN
                     LMPNPAKDVFVSDIYLPEKTQLSMKVFNTIGQLVMEEQWTLDAGYHKQATQVSTLAKD
                     IYNIRYTSNSFNIVKQIAIVK"
     gene            96547..97050
                     /locus_tag="PKX59_00425"
     CDS             96547..97050
                     /locus_tag="PKX59_00425"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOZ89356.1"
                     /translation="MSEIVLRSLQIDDAEIFALHANNSAIADNMTNQFPFPYTKAHAL
                     EFIRRVNGFKPAQVFAIDLDGEAIGAIGLHPQTDIFLKNAELGYWLAEKYWGKGFVSI
                     AIKKIVEYGFSSFEIDRIFARPFGSNKASQRVLEKTGFVLEARLEKIIYKNNRYEDEL
                     IYAIRRK"
     gene            complement(97421..98305)
                     /locus_tag="PKX59_00430"
     CDS             complement(97421..98305)
                     /locus_tag="PKX59_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89357.1"
                     /translation="MENQSKGNNRIWIVLFAASALFNVYQWRNHTSTTEVFEVTKDSL
                     ITARVDVEKELGATIEELNKYKGINDNLDSLLTEANHKIDEQKERIQKLIRSEGNSAA
                     LNKKLMAELESLKKLRDEYLDKIDTLMVENQKLKDDNAQLSGTVETLSKNLETTVNTA
                     SVLKSEYFKVSTFKKKGNGKFVSTALSKRTNKMDVCFSVLENKIAKAGEKNVYLRIIE
                     PGGKVMGNRSAGSTSFKMDSGEEVMCTVVSSLRYENATVDNCLSYEENDRIFTPGTYL
                     IEVYIDGNLSGASSYVLR"
     gene            complement(98414..99451)
                     /gene="tsaD"
                     /locus_tag="PKX59_00435"
     CDS             complement(98414..99451)
                     /gene="tsaD"
                     /locus_tag="PKX59_00435"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOZ89358.1"
                     /translation="MTVTDKKDIIILGIESSCDETSAAVSRNTQILSNVIANQTVHQK
                     YGGVVPELASRAHQSNIVPVVDQALREAAINISDVDAIAFTRGPGLIGSLMVGVSFAK
                     SAAAALNKPLIEVNHMQAHVLANFLSLEGQEGKTPSFPFLCLTVSGGHTQIVRVTSPQ
                     QMEIIGQTLDDAAGEAFDKIAKVFELPYPGGPLIDRYAQSGNPKAFNFPVANLPGLNY
                     SFSGLKTSVLYFLKKQLAQNPAFIAENRDDLCASVQYTIVKTLILKLKKAALETGISN
                     IAIAGGVSANSGLRKAVTEEAVKNNWQVHIPPLSLCTDNGGMICLAAYFKYLAADFST
                     LDVAPSARFGF"
     gene            99697..104073
                     /locus_tag="PKX59_00440"
     CDS             99697..104073
                     /locus_tag="PKX59_00440"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOZ89359.1"
                     /translation="MLLLLFLFSVSLRFQKVQSWVVDRVTGYLSSELNTTVHIGGVRF
                     VLIKSVYLDDVFIADKQQDTLLYAKHLTATVAFQSLFIRNKKLILDEVQLDKARIALS
                     KSKSTRDMNFDFIIDYFSSPSKDTTSSPFYFAVNRVILSNIHFTYRDFKWDDKTPCID
                     FEDIDVSDFNGTLYNIKPTDSSFSFYGSKISLKEKSGFKINSFSTYAAFYNDSMSFNK
                     ILLRTPYSSIDAGHYSMSYNSMTDFEDYINKVMMKGEFNESTISSNDIQYFAEELFGL
                     DKRVEFSGTARGTVSNLKTKKLKIAYGKSTRFEGDVSLNGLPDVNTTFINLNIKHLST
                     NKTDIETIKQYPFTEQQYIKLPDNISELGKIVYRGVFTGFYNDFVSYGNITTAIGNLS
                     TDVNLKFKNKANESTYSGKLTADNFDIGKFWRLGNAVGTISFNAQLNGTGFTSEKVNA
                     RLTGLVSHVNLNGYDYSNINLDADVARKLFNGTLNMNDRNAQVSFKGSIDFREKLPLF
                     DFNAIVKNAALTKLNLLKRDTSALLSTEANLNMRGFNFDEFEGKLQLKNISYSEEYKS
                     AFVEKILIQSGMENNNRNLNVNSDLLDLNINGQYILSKIVYSFGHLLNCHLPPFMLKL
                     SPDVEKTNTIFSFSGNIKDVNPVLNIFTPGLSVSNQTEFSGRLNRQHCEIEVDATSPW
                     INYKNFENKNIKLTSNNKNNLLNIDLSASQVKISDSLILKNILLDAETQKSKSSIVLT
                     VANDSLSTSKMRLATEQTYTNGATRFHLLESLLQFKGEQWQVDPANEILFDSASISFD
                     KVLFTNKSQSLMLQGKVGNNKEDVLSVNLFKFKVPLINDLLAMYDIKLGGAASGNLNF
                     SSLYQRAIITGEMRVDSFAFNDEPLGNAIVNFDWLTDQKKLHIEGAIEQNNRNSAVVK
                     GDYIFQPKNDELSFNIKVDKLSITPLERYLNGFASKIVGLATGELNLTGLAKAPNLTG
                     KARLQRASMVIDYLNTRYSVLPEQDIIFDEKYIAFNKVGISDDQNHKGIVDGKIFHNH
                     FNDFTFSLRVDANKLQCLNTNASQNELFYGKAFATGYAKIIGTLNLMKLDVVARSESG
                     TQIFIPLSNPEEVTQSNYINFINVNDTAAKKFVVTNKVDLSGIQMDLQLEATPEAEIQ
                     LIFDSKVGDVMRGNGSGILRMQISQAGEFSMYGDYNILAGDYLFTLQNIVNKRFKIQS
                     GLIQWAGSPYDATININAKYNLRASTFDLIPDSSNRSRVPVEVLLQLKNNLMSPDVIF
                     DVNVPNATPAVRTSLSSILGNEREMNRQVFSLLVLNRFSPPEGKNTSEESGSGNAVGQ
                     NLSEFVSQQLSNWASQLSDKFNIGVNYRPGDALNRDEFDVSISTELFRNRVAVESNVG
                     VANNSNQTSSIIGDFQVEFKISKDGNLRAKAFNKTNTNNLINNLNSQYTQGIGVFYRK
                     EFNTVSDLVESFRKRKKAAAPN"
     gene            complement(104070..105266)
                     /locus_tag="PKX59_00445"
     CDS             complement(104070..105266)
                     /locus_tag="PKX59_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89360.1"
                     /translation="MIKEIRDKYLREFKEETYSEFLAYYDNLYNRKVEFRIAESPVFI
                     PKKLKDLLIEAGNEILSQSLTPEYLLKSEQAIPHGLKVPNEHKHPHFIAIDFAICKDD
                     NGNYLPQLIELQGFASLFFWQDLLPRTYAKYFQIPEGFSHFFDGWNTEKHRALMRKVI
                     LGNHKPENVILLEIEPEKQKTWIDFWETKTHTGVEPVCISKVIKEGKNLFYELNGVKT
                     PIYRIYNRIIFDELLQRKDLIKDFNLTDDVDVEWAGHPNWFFRISKYTMPFIKSRFAS
                     ECYFLSDVDTSKLSLADFVLKPLFSFAGSGVLIDVTSSDIESIPLEKRNEFLIQRKVN
                     YVPCMETPDGAAKVEVRLLYIWDDAETKPQLVLNLTRLSKGAMIGVSHNKNKTWVGGS
                     SSLMEI"
     gene            complement(105327..105731)
                     /locus_tag="PKX59_00450"
     CDS             complement(105327..105731)
                     /locus_tag="PKX59_00450"
                     /inference="COORDINATES: protein motif:HMM:NF023145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agenet domain-containing protein"
                     /protein_id="HOZ89361.1"
                     /translation="MKKLIFTSLFFLGFLTFTYAQKVNEKVQINSKGAWYPGKILKIN
                     ADDHTYYVSYDGWDEGWNEWVTIDRLKDFAKEAPAQPLTKFKVGDRVEVEYGMVPEPA
                     TVIEVGENKYHIKYDKTVFKDRWVTEREIKKL"
     gene            105879..107135
                     /locus_tag="PKX59_00455"
     CDS             105879..107135
                     /locus_tag="PKX59_00455"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOZ89362.1"
                     /translation="MVQEKRALIFNYLFWLTGFLLPFDQPWINYALGLLLLFFVVQWL
                     TGKYQIKIQNPLLWLPLCYFLLHCIGMLYTQSQYTGWIELSAKSSFLIFPLLFSSNAI
                     SRDIIKTFKLFFIAGSGIICLYNLLLSFNQYIQHGNMSAFYYVAFSKGMHVQYLTIYL
                     NLAILFILEILFDASSQTKPIKKAYWVLLVLFFLVNITLLASRLATVVSYATILFYTV
                     VRIISSPNYLRYSILAALFAASVLIIDYFAFKKINRFEQVEIFLKENTNYVDFSKTEY
                     NSTTLRIPLWINAYEVIQRNPLFGVGTGDVKSSLDSMYLKNKFTYAHQNHFNPHNQYL
                     QTGVAFGLTGISLLMLMMLIPLYYAIRNRHYIFIAFQCIFLLNMLTESILERQAGIIL
                     FCLFYAIFSSEYVAAALKTVPEQNNT"
     gene            107158..108390
                     /locus_tag="PKX59_00460"
     CDS             107158..108390
                     /locus_tag="PKX59_00460"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOZ89363.1"
                     /translation="MKVPFAPPRMDQRVIDEVADTLLSGWITTGPKTKKFEKLLTAYC
                     NVPNVLALNSATAGLEIMLRWFGVKEGDEVILPAYTYSATANVVIHCGAKPVFVDVGD
                     DFNILPSEIENAINKNTKVIMPVDFGGLPCDFQKVNNIFQSENIKNKFVPATDVQQQL
                     GRILVLSDAAHSFGATVNNIKTGALTDVSVFSFHAVKNLTTGEGGAIALNLPKPFDNQ
                     SIYDYLCIYTLHGQNKDALAKMQKGNWRYDIIEAGFKCNMTDIMASIGLVELERYAET
                     LERRKQIVANYNEAFAKYPWAQLPEFKSNWRESSYHLYPIRIKNCTEKQRDAIMQKMF
                     DADIAVNVHFIPVPMFSFYTKLGYSLQHYPKAKDNYEREISLPLFYNLTEEQQQYVID
                     NLIKAVNETLVHRPAINS"
     gene            complement(108479..108805)
                     /locus_tag="PKX59_00465"
     CDS             complement(108479..108805)
                     /locus_tag="PKX59_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89364.1"
                     /translation="MDKKIIQKAGKYFTVEEKHKIIQELISAQCTKVEIWEKYTGEVE
                     EHGQLLRWMRQLGYNTGIKTRRPNIVSNLYSMAQKRIKSDNPDSYREVKVGDESFENL
                     QCLALK"
     gene            complement(108869..109288)
                     /locus_tag="PKX59_00470"
     CDS             complement(108869..109288)
                     /locus_tag="PKX59_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HOZ89365.1"
                     /translation="MADIFSKRKRSEIMASISGKETKPEISVRKFLFKEGFKFRKNVK
                     TLAGTPDIVLPKYQTIVFVHGCFWHGHNCNKAKLPTSNAKFWKTKIQNNIERDKKVKA
                     ELKKSGWTVLTIWECKLKSNKLFENSMNKLREELNAK"
     gene            complement(109281..111026)
                     /locus_tag="PKX59_00475"
     CDS             complement(109281..111026)
                     /locus_tag="PKX59_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ89366.1"
                     /translation="MKARNAFRNFIEYIQFPYFRNLEKNTRIIFDFPLTVFVGQNGSG
                     KSSTLHALYGAPKKNTPFEFWFSTAVDPIVEVSEGGDRHSFFYVYKDNRGRPLEVLKQ
                     RIFKRNNPDYWETARPNISYGMQKIAEGKRNQPIEKDVVYIDFRSELSAFDKYFYFKK
                     PRKSKTINNKQDYLRKHSVFLRRIIDGTNVIIHRYGENKNEQLKTFTAIELQHISTIL
                     GKKYVSGKFVKHKLFEEWGYSVIFETASYSYSEAFAGSGEMAVVKLVLEVLGAKEYSL
                     ILLDEPEVSLHPGAQKRLKQFLLEETRRKRLQVVISTHSPSIIEELPPNAIKVFSLLP
                     SGKFNVKNEITPDEAFYHLEQTNPARKTIIVEDLLSKQIIDGVLRELGQATASLFNVT
                     FYTGGESVIKEHFISVYSHANERNKFIVFDGDQRHAHFNPDSIVAAEQTNERYQQEIQ
                     NQTGVNVKFYPDGNPNAGARADQEKVLREKYLKFYLRNVSYLPLSVPEEIIWNQVLAE
                     TILTAMGKIEYIQRINATQDFKEKFLLVAEAMTGDKRNIEASEKTFLEKWLSDKPNSY
                     NDIVTIIESIRALNG"
     gene            complement(111068..112696)
                     /locus_tag="PKX59_00480"
     CDS             complement(111068..112696)
                     /locus_tag="PKX59_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HOZ89367.1"
                     /translation="MSNDKLYMTFEANTIKHLGVKMYSNMPPALAELIANAYDACATE
                     VHVRLYDEGEKKVIVEDNGTGMSFAEVNDYFLRIGRNRREEGQKSSCDRIPTGKKGLG
                     KLALFGIGDTINITTCQGKSGVSFNLDWNEILAWKGKDYEPRFDHLSEKEIFKGTRIT
                     LTNLKRKTGFPLEEYAESIAKLFNFQDKFIVYLLLNDSKPIKIDNKLKYDNITPEFEW
                     DIQEIIKVNEKDYSDKVQIHGTIITTEKPLKAALRGVTLFANGRMVNTPEFFGPSESS
                     HFYSYATGWLNVDFIDNWEEDVISTNRQSIDWENAKTSALRKYIASCLSIIERQWREF
                     RKEKKKKKISEKVNLNVKDWLEKLPQNVQGQVETIVNLLDDTPEISEDTQQVVTRLLH
                     DIAPEYANLHWRNLENEIKVVSEKYYQSADYYTAFIEALKRYTSEVRKKSGSTNGSER
                     SMMGEVFSGRKLSVTKKYKKTDGTDFTKDTITNIEEGQHYLSEGIVAGGRNPLQHEEH
                     LELSKTGLFSEKDCLDFLSLLSHLFKRLNDSEKP"
     gene            complement(112693..113769)
                     /locus_tag="PKX59_00485"
     CDS             complement(112693..113769)
                     /locus_tag="PKX59_00485"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOZ89368.1"
                     /translation="MKGKKSKTKRKFKAVDFFCSGGGMTSGFRKAGVNVVGGIDIDLE
                     CKETYTTNNPDSKFIHADIQDLAFSRIEKELKVKRNDKKMIFIGCSPCQYWSKIQTDK
                     TKAHETKNLLKDFQRFVDYFKPGYIVVENVPGILNRLDESPLKSFLDFLEKENYHYKY
                     KVINASHYGVPQTRRRFLLIASRVTDKISLPLPDTKTKLPTVRNFIGNKKVFKPITAG
                     QKDKTEFLHSSAGLSDNNLKRLLLTPHDGGTRTSWKDTDLQIPAYVGKDNSFQDIYGR
                     MFWDKPGPTITTKFYSITNGRFAHPEQNRAISLREGATLQTFDTDYKFLSTSIASTAR
                     LIGNAVPPQLSMRVAASIINCNPK"
     gene            complement(114268..114858)
                     /locus_tag="PKX59_00490"
     CDS             complement(114268..114858)
                     /locus_tag="PKX59_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89369.1"
                     /translation="MKKLIPITLISIVFFLSSCGTGKKSVSTTTTTTAVTPGFKITAP
                     DSITKTPVSAMITPAYTTTIHSSNFIVESASAWGAYEFTQAGDYKIVIMDVGSKTDEG
                     EKEFVNFCLEGQAVNKHFEDLKIIRPEVTNGGRCINGLAQNLVSNEYVVRGIKPGDQI
                     KAWPSRNGATMRRTGLNGTQAEWDGDGRLKVLITPL"
     gene            complement(115583..118957)
                     /locus_tag="PKX59_00495"
     CDS             complement(115583..118957)
                     /locus_tag="PKX59_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOZ89370.1"
                     /translation="MQKKTIVTTFFIALFALQGNAEWVKITSDNPSKSAVIVSGTPDN
                     TVMEISVSGFEKHRVKSLAGEYYTISSPEMTPRMEEGTPDLPKFALSVIIPDDGDMSL
                     NIESIEELTFQNIQIAPSKGNLYRDIKPSDVPYRFGDIYSQNVFFPANSGFLRSPFIL
                     RDFRGQTVVVNPFSYNAATQTLKVISKFKVTLQSKAGTSVNTLSRTTSLQKVDRDFAN
                     IYQNVFRNAGSFINYLPVNEDGEMLIISDASFMAAMQPFIDWKIKRGLQVSMVDVATA
                     GSTAQQIKTFIQNYYSTHNLKYVLLVGDAQQIPTPVLSGGASDPSYGYLVGNDSYAEV
                     FVGRFSAQTVADVTTQVQRTVDYEMLPTPGVNWYSKGICIGSDQGPGDDNEMDFQHQQ
                     VIRGKELAFTYTDVAELYDGSQGGNDMSGNPTAAMLSSEINNGASIITYTGHGSSASL
                     GTTGFSNANATALTNTGMLPFIWSVACVNGEFANATCLAESFLRAQYNGQPSGAVATF
                     MSSINQSWNPPMDAQDEMVDLLVGTNSSNIKRTFGGLSMNGCMHMNDQYGAAGFEMTD
                     TWHCFGDPSLNVRTATPYAITASHSPVLFPGASQLNIACNTDSALACLSSNGQILATG
                     LVIGGNVILNFNTITSLDTLYLTITAFNGIPYMVAIPVVATTGPYVSLTSNVINDAGG
                     NANQLADYNEIIDVDLMLENFGQGDAVGVGLTVTTTCPYVTINSSSLNKVNIQSGNAA
                     GITNAFNYTVAAFVPDNTPAVFDIVLTDSAGTVWSTSITHLLKAPMLQVYSITVSDPT
                     GNNNGILEPGETADVTIKTNNKGHSATTASTATLATSSPYLTINNTSYNTGVVQALSD
                     VDATFNVSLASNIQIGSTFDMQFNIDANPYSDNKTFNMVAGEILENFEYGNFTTYPWL
                     NNGSHPWTITNSGTFQGAYSARSATIADGDTSDLAITVNVYNNDSISFYMLISSEMGW
                     DFLSFYIDGTLIDSYSGVYGWAYKSYPITLGQHTLTWAYIKDEVCCTGGQDLAMLDNI
                     QFPPSTMVTSAKDFSSINSVTVYPNPAQDELTIITKDFKNVEVKLFAMDGKLVYSAAN
                     IISDVYKMPLNDISQGMYYLQIKADNKFYNSKIIKR"
     gene            119138..119779
                     /locus_tag="PKX59_00500"
     CDS             119138..119779
                     /locus_tag="PKX59_00500"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89371.1"
                     /translation="MAVYRFKVSFEDFEDIYREIEVTSEQTFADFYKAILSSIKFEDR
                     GTVSFFMSNDSWKKGVEISNNDKTDRDGKPVAKTDKAFLKSYIADPHQKIYFLIDCES
                     VWAFHIELIKIMAAADVKLTYPHIRKVSGDAPSQFKVKVAPVPDEGDEVLDSDTNEDD
                     NFSSTANEIAGENDYDEDDTAALEGEEGEESSDEDSEEFNDVEMGSDENYEEN"
     gene            119840..120841
                     /locus_tag="PKX59_00505"
     CDS             119840..120841
                     /locus_tag="PKX59_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89372.1"
                     /translation="MKAGDKVSFKDEKLNGVILEILQNGKVLVELEDGFPMEALQNQL
                     VVTVPFIKQQTQNDAKEDISEQNTSTENIPFDLNNNIIDFYAVPSKADRVLTGSVDHF
                     LTNGTPNLLLLIFGKRSKNQHTILYKGELKPNSYVCLSIFKRDALINLDSFYIQILIA
                     ADGEYKRPFTKDIAVELPELNQTKNTETGILSSSKKVSLVNLNVADEIQIKDLLEKFD
                     SSQPAKTSDIKKNKKQQPISDSDISKEIDLHINKLTADFQNMTNSEMLALQINTFRKE
                     MDNAIIKDYKQLIFIHGIGDGILRNAIMNETKNYSDIKVFMANQARYGTGALEIRFL"
     gene            complement(120836..122581)
                     /locus_tag="PKX59_00510"
     CDS             complement(120836..122581)
                     /locus_tag="PKX59_00510"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HOZ89373.1"
                     /translation="MMHYRFSVSNAASHFVDVELTIQTDGNETLLKLPVWRPGRYEEG
                     NFAKNIRNFRVQDINGKEVKWKKVSKSSWLLKTNKNDTIKINYQYYCNQLDAGACFVN
                     DEQIYINPIHCCFYTDANRGNACTVEIVSNFSRVATSLTQTEKNVFSAKNFDELVDSP
                     FIASDDLMHYGYTVSDCSFHIWIQGNFKPDVEKLIEDFKSFTQIQLNVMKSIPVKDYH
                     FLIQAVPHTFYHGVEHLASTVLVIGPSAKLNDRELYNELLGVASHELFHVWNIKTIRP
                     VEMQPYNYDSPNYAETGFVYEGATTYYGDLFLARCGYFNEEEYFNELNIRINKHLSNE
                     GRFNYSIAQSSFDTWLDGYVPGIPGRKTSIYDEGSLVSLMLDFLIRKYSDGKNSLDDV
                     FIELYNAFGKKNIGYTSDDYLRIASEKAGRSLETFFNDIVYKACSYQSLLEEVMLFAG
                     LELEENPSKKNAERYFGFKTNDNAAMPEVISLPIGSPAFENGLAIGDLIIGVDGQKAD
                     KNYDMQINPKENHKTGFLISRGNRIKTIEMMPASKKYFSTWKVKHISKKNAEQEQFFN
                     QWTNNLRAHHSQNSL"
     gene            complement(122583..124661)
                     /locus_tag="PKX59_00515"
     CDS             complement(122583..124661)
                     /locus_tag="PKX59_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOZ89374.1"
                     /translation="MIAEKKLESTPISTETVLKAFRLMCTARAMSDLYEEHATFTARY
                     VHACSRGHEAVQIALGLQLKSIDYLSAYYRDDSILLSIGMTPYELMLQLMAKRDDPFS
                     GGRTYYSHPSLRRDDMPKIPHQSSATGMQAIPTTGIALGMHYLENQNLVEYAEGEYPV
                     TVCSLGDACVTEGEVSEAFQMAVLKKLPILYFVQDNEWDISAHASEIRTMNAAEYAKG
                     FKGLETRSIDGTDFFQSYNTIKEVLDIIRTERRPFLIHAKVPLLGHHTSGVRREWYRH
                     DLEEAKKKDPLPKFIQQLEEAGFTSDELDAIKKETRTLVEDDFAKAKAADDPLPEDLF
                     KHDYAPTTVTEEKGVRQPAGKEPTVMVDCALFAVKELLQKHPECLFYGQDVGARLGGV
                     FREAATLAKTFGDDRVFNTPIQEAFIIGSTVGMSAVGCKPIVEVQFADYIWPGLNQLF
                     TEVSRSCYLSNGKWPVSCVIRVPIGAYGSGGPYHSSSVETVVLNIRGVKVAYPSTGAD
                     LKGLMKAAYYDPNPVVIFEHKGLYWSKIKGTEDARTIEPDEDYIIPFGKARIVQQAEA
                     LQDENTLTVITYGMGVYWAKAAAQEFKGRIEIIDLRTLNPLDETTIFESVRKHHRCLV
                     LTEEPLKNSFAEALSGRIQQQCFTALDAPVVAMGSENMPAIPLNSTLEFTMIPNAQKV
                     SEQMNYLLNY"
     gene            complement(124685..125449)
                     /locus_tag="PKX59_00520"
     CDS             complement(124685..125449)
                     /locus_tag="PKX59_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOZ89375.1"
                     /translation="MTEGSVESIIKDQVAEITFFHPQSNSLPGVLLRKLAATIEEAGA
                     NESAKVIVLRSKGEKAFCAGASFDELISINSESQGKEFFSGFASVINAMRKAPKFVIA
                     RVQGKAVGGGVGLACSADYTLAHVSASVKLSELAVGIGPFVVGPAVERKIGTGAFTQL
                     TVNATEWQNAQWAKEKGMFASLHDNIEDLDKAVHKLAQQLAASNPEAMKMLKQIFWKG
                     TEGWDALLMERAGMSGKLVMSDFTRIAIEKFKTKAG"
     gene            125694..>125953
                     /locus_tag="PKX59_00525"
     CDS             125694..>125953
                     /locus_tag="PKX59_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOZ89376.1"
                     /translation="MQFNIPAHLTGLTDKEVDSSRLKYGINQKKTDTRNSIFNWILDL
                     LKEPMLILLLAVSVIYLIIGDHGEAIFMFIAIVAVSAISFYQ"
BASE COUNT        40037 a        22798 c        22601 g        40517 t
ORIGIN      
        1 cagtataaaa tagaaaaatt acctgttgtt gaccgttcgg gaaaattaat aggattagtt
       61 acttataaag acatcataaa atttaagtca cgacccaatt catgcaagga taaattaggg
      121 cgattgagag ttgcagcagg cattggtgtt acagcagata tgcttgatcg tgttgcctca
      181 ttggtaaatg caggcgttga tgccatagtt attgatactg cccatggaca ttcaaaaggt
      241 gttatcactg cattgaaatc tgtaaagaaa aagttttcag gattagatgt tgttgtaggc
      301 aatgttggaa ctgctgaagg agccaaacta cttgttaaaa atggtgctga tggcgttaaa
      361 gtgggtattg gtcccggatc tatctgcaca actcgtgttg tagcaggtgt cggagtgccg
      421 cagctaacag cggtcgctga tgcggcaggt gctataaaag gttccggtgt tcctattatt
      481 gctgatggtg gtattcgttt tacaggcgat attcctaaag ccattgcagc aggagcaaac
      541 agcatcatgg caggttcaat gtttgcaggt gttgaagaat caccaggaga aacaatcatt
      601 ttcgaaggaa gaaaatttaa gtcttatcgt ggcatgggct ccattgaagc aatgcgtgaa
      661 ggttcaaaag atcgttactt tcaggatgca gaagatgata ttaaaaaatt agtacctgaa
      721 gggattgtag gtcgagttcc atacaaagga acacttgctg aaacggttta tcagttggtg
      781 ggtggactaa gggcaggtat gggttactgt ggtgcaaaga atattgcaga attacagaaa
      841 gctaaattta tacgcatcac acaaagcggt gtcagagaaa gccatccaca tggtattaca
      901 attgtaaaag aagcaccgaa ttacactaga tagttttttt gttatcaggt ttgatgtgga
      961 ctaatttgtt cgcagtacaa ttaactttgt tttattaatt gacagtgcaa gggaaaaaat
     1021 aaagaagttt atttactaaa gcactaagaa ctaacatgaa atttttaaat tggtttttta
     1081 tcgtgctttc tctctgtatt ttcagagtat tttcgtaaca gaattaaaat tctatttcag
     1141 gaaaatttgt cttgttttaa tttacgttta tgtaaaaatg actgttgtat gaattcgttt
     1201 ctgacgaatt gactgtttta tattcgcatt tcagtattgg ttaaatttgt gatagtgatt
     1261 caacaaaagg attaaaatgg atataaataa attaacaatc aaatcgcagg aagtattgca
     1321 gcaagcacag tttctggcta aatcatttgg acagcaaact gtagagacag gccatcttct
     1381 aaaatccatg cttgaggtag atgaacatgt tgttcctttt ctattaaaaa aagcaagtgt
     1441 aaatatcaac tcattgacac agaaacttga caatatcatt caatcatatc caaaggttag
     1501 tggtggtcag gtttatttat caaccgatgt taacgatgct atgcaaagag cattcgctgc
     1561 attaaaagta aataaagatg agtttgtttc tgttgaacat ttattactgg cattactttc
     1621 aggcaaagac aaagttgcaa agcttctaaa agaagaaggt tgtaatgaaa aggacttaca
     1681 aaatgcaatt aacgaattgc gaaaaggtga acgtgtgact tcagcaagtg cagaagaaac
     1741 atacaattcg ttgaataaat atgcacgtaa cctgaatgag ttagcacgta atggtaaact
     1801 tgacccagtc attggacgtg atgatgaaat aagaagagtg cttcagattt tgtcaagaag
     1861 aacaaaaaac aatcctatcc tcattggaga acccggtgta ggtaaaactg ctattgcaga
     1921 aggtttggca catagaatta tcaatggtga tgtacctgaa aatttaaagt cgaaggttat
     1981 ttactcattg gacatgggtg ctttaattgc aggagcaaaa tacaaaggcg aatttgaaga
     2041 acgattaaaa gctgtagtaa aagaagtcat cagtggcgaa ggagaaatta ttttgtttat
     2101 tgacgaaata catacacttg taggtgccgg tggtggcgag ggtgcaatgg atgctgccaa
     2161 cattctgaaa cctgcattgg cacgtggcga actgagggct gttggtgcaa caactttaaa
     2221 tgagtatcag aaatacattg aaaaagacaa agcgctagaa agacgttttc agaaagtgat
     2281 ggttgaagag cctgatacag aatcggcaat ttctattctt cgagggctta aagaacgtta
     2341 tgaaacacat cataaagtca gaataaagga cgaagcaatt attgctgctg ttgagctttc
     2401 gcaacgttat attaacgacc gttatcttcc cgataaagca attgaccttg ttgatgaagc
     2461 atcttcaaaa ctaagacttg aaatggactc tgttcctgaa gaattagatg agttgaacag
     2521 aagaatcatg cagctggaaa ttgaaagaga agccatcaaa cgcgagaacg atactaaaaa
     2581 agtaaatgag ttatcagagg aaattgcaca actcaacgaa cagcgaaatg ctgtaaaggc
     2641 acgctggcag agtgaaaaag aagtggttga aaaaattcag gatataaaag agaaaattga
     2701 aaatcttaaa ttagaagcag aacaagcaga gcgctccggt gattatggta gagtagcaga
     2761 aatccgttat ggcaaggttc gtgaagcaga agaaacactt tcaaaattga tggaacaact
     2821 caatgaaatg catagcaaag gtgccatgat gaaagaagaa gtggacagtg aagatattgc
     2881 cgaagttgtt tcacgctgga caggcattcc ggtgaacaga atgttacaga gtgagcgtga
     2941 gaaactcttg catcttgaag cagagctgca taaacgtgta gttggacaag atgatgctat
     3001 tgaggcagtt gctgatgctg ttagaagaag tcgtgcagga ttacaagata tgcgaaagcc
     3061 aataggttca ttcatatttc ttgggtcaac aggtgtagga aaaacagaac ttgcaaaagc
     3121 attagctgct tatctgttta atgacgaaaa caacatgact aggattgata tgagtgagta
     3181 tcaggagaaa catactgttt cgagattaat tggtgcgcct cccggatatg ttggctatga
     3241 tgaaggtggt cagttgactg aagctgttcg cagaagaccc tattcagtga tattacttga
     3301 tgaaattgaa aaagcacatc ctgatgtatt taatattctg ttgcaggttc ttgacgatgg
     3361 caggctgaca gacaataaag gccgtactgc taatttcaaa aacaccatca tcattatgac
     3421 atctaacatt ggtgcacatc tgattcagga aaattttgaa aacattgatg aaagaaacag
     3481 agatgaagtt attgcaaaga caaagaacaa tgtgtttgat ttgcttaaga aaaccatcag
     3541 acctgaattt cttaatcgta ttgatgaagt gataatgttt aatccgctta caagaaatca
     3601 gatagaggag atagtgcgta ttcagtttaa tcaggctgtt gatatgttgg caaagaacga
     3661 cattaagtta tcaattacag atcgtgcaat agagtgggta gctcagttag gatacgaccc
     3721 tcagtttggt gctagaccat tgaagcgtgt actgcaaaag aaaattctca acgaactatc
     3781 gaaacagatt cttgcaggaa caatcaataa agaggatgtt attctgattg accttgacaa
     3841 aaagaagaat tttatttttg aaaacgaaaa gacacataca gaaataaaag cgtaacaatt
     3901 ataatagccg ggattaaaag ttccggcttt tatttttcaa cgaaaacaaa aatatcttcg
     3961 tcatcttttt tcatcagata tttttctctg gcaaactttt caaggtttgc aggagatgaa
     4021 atcaattcgt tcaaatcaga tttacttttt tcaagttcat cagcataata acttttgtca
     4081 ttctttaaag tattcaactg ttgcctgaaa cgatattgtg cgtaaaaatc gttcttgtca
     4141 aaaaaagcaa gccacaccac aaatgctata atgcaaattg catatttgtt tttcaagaaa
     4201 tcgggtactt tttcccacgc ttttttcatg acctaaagtt acttgttcat ttaacttaaa
     4261 taaccttgtt atttacaagt tatcagaacc aatttgttaa aagcgttatt tcaaattgag
     4321 ctgacaaatg gtttgttttt caataaagta ttacagtcgc tgactaactt ttttcaccat
     4381 taaatttttc catgaaagaa aacttccatc attaattctt tttcttgctt ctgtaactaa
     4441 ccaaagatag aaagacaggt tgtgaagtgt tgctaattgt gcggcaagca tttctttgct
     4501 gataaataaa tgccttaaat aagctttgga ataatctgta tctgcaaaac aatttcctga
     4561 ctcatctatg ggcgaaaaat catttttcca tttttcgttt ctgatattaa tgataccctc
     4621 agccgtaaaa agcataccgt tacgtgcatt tctggttggc ataacacaat caaacatatc
     4681 tatacctaaa gcaatacttt caagaatatt tgctggagtt cctacaccca tcagatagcg
     4741 cggtttttca acaggtaaaa tatctgtaac cacatcagta atttcataca taatttcagc
     4801 gggctcgcca acggatagac caccaattgc attgcctttt gcaccggcat cagcaattac
     4861 ttttgaactt tctgtgcgca ggtctttaaa aacagatcct tgaacaatag gaaagagtgt
     4921 ttgttcgtaa ccatactttg ccggagattt ttcaagatga gatatgcatc gttgtagcca
     4981 gcgatgtgtc atttccattg acttttttgc cgttctatgc tcgcatggat agggcgtaca
     5041 ttcatcaaat gccatgatta tatcagcacc aataaaacgc tgtatgtcca tactcacttc
     5101 aggagaaatg aaatgtttgc ttccatcaat atgtgagttg aatttaaccc cttcttctgt
     5161 tatctttctt gtttcgctta aagaaaaaac ctggtatccg ccactatccg ttaatattgg
     5221 tagcgaccaa ttcataaact tatgcagtcc acctgcctga tgcaaaactt caatgccggg
     5281 acgtaaattc agatgatagg tgttgccaag aataatctgt gctttaacat cctgttttaa
     5341 gtcgcgctga ttaatgcttt tgactgttcc tgctgtgcca acaggcataa aaattggagt
     5401 ttcaataatg ccatgagcgg tttctattct gcccgagcgg gctttacttt ctttatcata
     5461 cttttcaata gaaaaatgca tagattcagt aatttttgca aaaagtgttg ttaatatttt
     5521 tacaaagata gaaaagcctt ctgcaatgac tcttcataca tttgcgcgct taccaacacc
     5581 aaagttattt tgatacccat acctatacca gattttataa gcattgaaaa tatttcattg
     5641 gtactttttt atttgcttgt agtatttatt accatacaac taatctatta tttatttgtt
     5701 tttgtgcgtt tgtctctgca taaaacagta aacgccccag tgtcagaaat tgagccgcct
     5761 gtttcaataa taatttgtgc cagaaatgaa ctgaaaaacc taaaggcata tcttcaacaa
     5821 gtattggagc aagactatcc tcgatttcag gttatagttg tgaatgattg ttcgtgggat
     5881 gaatcagaaa agtatcttga agaactatct gcaacctata caaatcttaa agtagtttca
     5941 atcaaagaac aggaacgtta tcagcatgga aaaaagtttg ccttgacaat tggaattaag
     6001 tctgccttat atgaccatct gttgttgatt gatgcagatt gttatccggc aagcaatcag
     6061 tggatacgac tgatgatgca aagttataat gcaaaaacaa gaatagtaat tggttatggt
     6121 gcctatatta aaacttcttc actattaaac cgttgggtta gatttgacac tgcatttaat
     6181 gcaatgcaat atctttcatt tgcacttgca ggaaaccctt acatgggtgt tggaagaaat
     6241 ttatcatatc agcgttcact gtttttcgag aataaaggct ttgcccgaca tcaacatatt
     6301 atgtctggcg atgatgatct ttttataaat gatacagcca ctacagagaa tctttctgtt
     6361 cagataaacc ccgattcttt tacttattca cagccaaaaa ccgactttat tgactggttc
     6421 cgtcagaaga aaaggcatat aagtgcttct cgcttatata agtttaaaca taaaatgctt
     6481 ttaggcattt actatttttc acatatttca ttccttgttt tatttatagc attgttaata
     6541 attcagttta aaattgaata tgtagttact gcctttagtt tacgtttggt ttttcaaatg
     6601 attatttttg gctcatgctt aaaaaaatta aaggaaggcg atctgattcc gttgataccg
     6661 atattcgata tatggatagc agttgtttat cctttaattg ccatctccaa tcgtttaata
     6721 aagaataaaa catggaagta aaaagtccca aaactgcaaa ggcagcacaa gataaaacag
     6781 aagttaatcc taacttaagt gataaagcag ttgtagatta tcagctggtt cgtgaagcag
     6841 tagatcatgg caatcaaaaa gcttatgctg aactaatgtc gcgctacaag gattctatct
     6901 attttatgct acttaaaatg gtcaataatc gtgacgatgc tgatgatttg acaattgaag
     6961 cttttggcaa agcatttaag aatattcatc aatatacacc tgactatgct ttcagcacct
     7021 ggctatttaa aatagcaacc aacaattgca ttgattatat ccgcaaaaag cgaaagttaa
     7081 tgttgagtat tgataaagga tatgaaaacg aaaatggcga agatgttgct ttggaaataa
     7141 aatctgacac cataggtcct gaagaaaagc tgatgaaaaa gcaaaaaatg gaaaccatgc
     7201 gtgaggttgt tgataagtta aaaccaagat accggacact tattgaactc cgttattttc
     7261 aggagttgag ttatgaagaa attgcaacga acatggattt acctcttgga actgttaaag
     7321 cccaactttt cagggcgcgt gaatttctgt atcagataat gaaaaactct gaacatcgtc
     7381 cataaaagat gtcaagcgga gtttcattat tgcaacgcta ctttccggaa ttacctttag
     7441 aaaagcagga aaaatttgtc ttatttgctg agttatttaa agtatggaat gagaagataa
     7501 accttgtttc cagaaaagat atagatcagt tttatttaca ccacttatta cattctcttg
     7561 tcatagcaaa gtttattgat tttggtaaag gaactaaagt catggacatt ggcaccggag
     7621 gcggattacc gggtttacca ttagctattt attttcctga aactaacttt tatctggttg
     7681 attctattcg taaaaagact gaagccgtta aagaaatggc caatcaactt tcattatcta
     7741 atgtaactgt atttaacaaa cgagccgaag atgtatcaga aaaagttgac tttgttgttt
     7801 ccagagctac ggcaccactc tcagatttgg taaaatggag taggggtaga attaattcta
     7861 aaggcaaggc atcaatccca aatggactta tttgtttaaa ggggggagat ttaaccgaag
     7921 agctaaaacc atttgaaaat aagctattta cagaaaatgt tacggcattt atacaggaag
     7981 aattttataa agaaaaaaaa ataatctact tgcctttagt ataaaagaat taattatatc
     8041 tttgccgcct ctaaaaagaa ttttgcagca ttaataaaaa ctgaagtatt atggtaagga
     8101 catatcaacc ctcaaagcgt aagagaaaaa acaaacatgg ttttcgtgaa agaatggcaa
     8161 ctgctaatgg tcgcagagtg ttagcttcac gcagagcaaa aggtcgtaaa aaactgactg
     8221 tttcaagtga aagtaaagtt cgtggtaaat aaataactac attataaaat atcaaaaagc
     8281 tgccttcttt aaggtggctt ttttatttta gcaccaatta ttaaagtttc ttgtaatcat
     8341 gaatttcaaa tcaggtctct tttatttgtc tattattctc ttgtttactg cttgtcagaa
     8401 agattataat gaccccggca ccggcccatc gcaatattat ggaacacgca gcggtgtttc
     8461 tatcagggct attttattgg cgcaaatgcc tgctacagat ccatcaggaa atagttggga
     8521 tgtagcagac tctgttgaca cagcaggtcg tgcagatatt tattaccgtt ttaaagacat
     8581 agactctatt gactatcctg aatttgttca gaattttcat tttgacaatg taaatcctga
     8641 tagtttgccg ctgccatatt ttttaactac gccttatcca ttacgtgcca ttggcagcaa
     8701 tgttcagttt gatatttatg attttgagaa gagcctttca tcgccagcta ttgactcaac
     8761 cttaatgaaa tcatttaact tttcagttgt tgcaggcgat tcactaattg agaatcctta
     8821 tcctgatagt ttggtactgg cagaagatgg ttatgtagtt ctgctttatt tgaactggaa
     8881 aaagtaatgt tgtgattatt tataaaaatg aaaagctgca ctctataagg gtgcagcttt
     8941 ttctaaaagt actttaatca attaatttat tgtaatcttc tttatacttg tttttcctgt
     9001 atctgtagta attttcaaca gataaatccc tttggaaacg tcattcaaaa caacattttt
     9061 agttaaaaca gaaacatcac taacattttc agagtagatg cttctgcctt gcaaatccat
     9121 tatattaatt tccagattac agcggtttgc aaaagaagca ttaatcataa aatttccttt
     9181 tgatggattt ggtagaatag taagtgcgtc ttctacttct attgtattaa ttgaagttgt
     9241 aatagttcca tatatttcaa aaggaaatcc ttgaggtgtt gatagagcaa catctaatgc
     9301 aggaagccac atagagctat caggaatata taataatccg tctcctgtag tagtatttaa
     9361 agtatcagtt aaaggcggta cccatggtcc tgagagagat ggatttccac cactctgcca
     9421 acttaaccaa taattacctg aagtaagtgt ccatgcactt tgtggtacaa ttacactgta
     9481 cattaccgga cgtgacacgg aattaaactg accatctaca gtcctgtaaa tatttgaaaa
     9541 gcgagtgttt tgtaacagat tagttattgt atcacctaaa acaattgtac cgcctgatgc
     9601 cggagagcca ttaaagattt cacaatttac agcatttatt gtagatacaa ttcctgaatt
     9661 actctgatat gcataaaacc taatagagtc aattgtccaa atttgacctg ccgggatatt
     9721 aaaatcatct gctattctat agccttcaag caattgttgc ccgaatccta atgaagctaa
     9781 tccatgatga aaacaggata tatctgcacc accttgacct acacccgggt gattaataat
     9841 agtgccattg gaataaatct gtgcctgacc ctgaatatag caaataagta ttacaaataa
     9901 taaagtaaat aattttttca tatctaaatt ttttacaaac ttaattcaat acccatataa
     9961 tacaattcaa ctatagaaat attcataggc taatcgagtt tgttctacaa ttttttaaaa
    10021 aaaaaggctg caatttctaa aaattacagc ctttaaaaag tttataaaat tattagtcaa
    10081 ctacgatttt cttggtaaga actttacctg aatcacttgt aagtttcaac agataaatcc
    10141 ctttggaaac gtcattcaaa acaacatttt tagttaaaac agaaacatca ctaacatttt
    10201 ctgagtagat gcttctgcct tgcaaatcca ttacattaat ttccagatta caacggtttg
    10261 aaaaagtagc attaatcata aaatttccct ttgatggatt tggtagaatt gtaagtgcgt
    10321 cttctacttc tattgaattg atggaagttg ttacagttcc atatatctga aatggtaagc
    10381 cttgtgggtc agctgtagcg ccaccatcca gaattggatt ccaaattgaa ccatcaggat
    10441 tgaactgaat tgcatctccg gttgccatat tagcattagt caaaggaggt gcccagggac
    10501 cagatgttaa tgtaccgcct gtttgccagg caacccaata agttccagcc ggtagtgacc
    10561 atgcactttg tggcacaact atgtttcgca ttaccggacg cgctgtattt gtaaatgctg
    10621 catccgctgt acgatagata tttgagaaat aagtgctttg catcaaatta gttattgtat
    10681 cgcccaaaac aatagaacca ccaattgccg gtgaaccatt aaaaattgca cagtttacat
    10741 ctgtaattgt agatgcgttt ccagaattgg tttgataagc aagaaatgca atagaatcaa
    10801 ttgtccatgt ctgacctgca ggaattatga aatcatcagc aacacgataa cccagtccgg
    10861 aagcatgatt catacctaat gaattcagtc catcatgtaa ggaggataca tctgctccac
    10921 cgacacctaa taccggatta tttacaattg taccattgtc atagatttgt gcattagaag
    10981 ttaaacttcc taaggcaact gctgtaataa gagtaaatag ttttttcata ttttttttga
    11041 ttttgaaatt atctatcaaa agtaaaggtt taaatattac aatcactata ttttacatta
    11101 gatttcttaa aatatcaaca ttacttccaa tgaatatgtt tataacatca actaaaatct
    11161 atctccgtac tatcaccaat attcaagctc tgacttagtc cttttagaaa tgcatcgctt
    11221 ccaattacag aacgatgtaa aactgcagat tctaactgcg aataggagcc aataattgaa
    11281 tctttaatga tagaatattt tacgatggta tgctcgccaa tagaaacatg aggaccaata
    11341 atggaatcag caatatcgca gttgtatgca atactcaccg gaggaattat gattgtattt
    11401 ggaaacttct gtggtttagg atttgtttct gcaattctgt ctaataaaat tgcattggtt
    11461 tcaaggagac tttccttgtt tccacaatca aaccaattgc ccacatgaaa gactttaatt
    11521 ttctcaccct cttcaatcat tcgcatcatg gcatcagtca gatgatattc atctcttgtt
    11581 ttcaggttgt tttcgataat gtaattcaat gcttcaaaca aatgccctgc ttgtttaata
    11641 aaatagatac cgacaagtgc aagatttgat tttgggatta ccggtttttc atgcaacttg
    11701 gaaacgaaac cctcatcatt caattcaaca acaccaaaat ttcttggatc atctactttt
    11761 ttaactccta aaagagactg ttcggcttgc ttaaaaacat taatgtcaaa atcaattatc
    11821 gtatcgccca atacaatgag catttcatct tctgcatgaa catggtttcg agcaaaccaa
    11881 acagaatgac cggtaccttc gcgtggttcc tgcaatacaa atgctgcttt aagtgaagga
    11941 tatttgcttt ttacaaattc ttcaattttt tcacccatgt aaccaataat aaatacaaac
    12001 tcatcatatc cggattgcac aaaataatca atgatgtggg caagaatggg atggcctgca
    12061 acaggcacta atgtcttcgg ttgtgtatgt gtatgtggac gaagttttgt tccgataccg
    12121 gccacaggga ttacaatttt catttcaaat tattttaagc aaaaatatta aactacagaa
    12181 cagcataagt ctgatggcat ggaataaaaa aaaatgtagt tttgccacac ttaactgata
    12241 tttaaacgca cgatatgttg aacaaaatta ccgaattact tggcaaggat gccgataaat
    12301 tattaaatca cgtttgtaaa actgttccaa aggaaacatt acatcagccg ggtaacgact
    12361 ttgttgaccg catttttatc ccttcgaaca gaagtaatca ggtaatccgt agcatacaat
    12421 cactctataa tcatggtcgt ttgaatggta caggatatat gtccattctg ccggtagatc
    12481 agggaataga gcactctgcc ggagcaagct ttgccccaaa cccaatgttt tttgaccctg
    12541 aaaacattgt aaaattagcc atagaaggcg gctgtaatgc agttgcttca acttatggag
    12601 tacttgcatc tgtttccaga aagtatgctc ataagattcc ttttattgtt aaaattaacc
    12661 ataatgagtt tctgacatac ccaaataaat ttgatcagat tatgttcggt actgttgatg
    12721 aggcttggaa catgggcgct gtggctgttg gtgcaacaat ttattttggc agcgaagaat
    12781 catcaagaca aattgtagaa gtggcacaag catttgaacg agctcacgaa ctgggtatgg
    12841 caacaattct ctggtgttat ctacgcaacc cgggatttaa aaaagacgga atagattacc
    12901 atactgctgc agacttaact gctcaggcaa atcatttagg agtaactatt caggcagata
    12961 ttattaaaca aaaactacct actaataatg gtggatacac tgctattaac ttcggaaaaa
    13021 ctcataagga tgtttatgca aagctttctt ctgaacatcc tattgacctt tgtcgctatc
    13081 aggtattgaa ttgttataac ggccgtatgg gattaatcaa ttcaggtgga gaatctaaag
    13141 gtgctacaga tatggccgaa gcagtaacca cagcagttat caataaaaga gccggtggtc
    13201 agggattaat ttcaggacgt aaagctttcc aaaaaccatt taaagatggt gttgaaatgt
    13261 taaatgcaat acaggatgta tatctttcaa aagatgttac tttagcataa gtaatataaa
    13321 cagtatatca gttgataaac ccaacattta aaaacgcaaa ctgattataa atgacttggt
    13381 ttaaacgaat taaagagggc atcaccacct ctacaagaga gaaaaaagaa actcccgaag
    13441 gtttgtggta taaatgccca tcatgtaaag ctattgttcc atcattggag catgaggaaa
    13501 ataaatatgt atgtccaaag tgcaattatc acgacagaat tggttctgaa gaatattttg
    13561 cagttctttt tgatgacaat cagtttacag agtttaatac agaactaacc tctgccgatc
    13621 ctttaggttt tgttgataca aaaaaatata ctgaccgctt acgtgatacc atttctaaaa
    13681 cgaagttgaa agatgctatg cgtactgctg taggtaaagt taacggtaac gatttagttg
    13741 ttgcttgcat ggactttcag tttattggag gaagtatggg ctctgtaatg ggcgaaaaaa
    13801 ttgcaggagc aattgactat agtattcagc ataagatgcc attgatgatt ataaacaaat
    13861 caggtggtgc ccgtatgatg gaagcagcat tttctttgat gcagatggca aaaacatcag
    13921 caaagcttac cagacttgcc gatgcaggct tgccttatat tagtttactt accgatccta
    13981 ccacaggagg tgtgacagca tcttttgcca tgcttggtga tttaaacatt ggagagcccg
    14041 gagcattaat tggatttgcc ggtccacgag ttgtaaaaga aacaataggt aaagatttgc
    14101 ccaaaggatt tcagacttca gaatttgttc ttgaacatgg ttttttagat aaaattgttg
    14161 accgcagaca attaaaagat atcattggtc agatactaag catgtttaaa aactaattgc
    14221 tttcagttta ctttttctga ataaataatt caactaatcc tttacatgtt ttagtttgtt
    14281 acaagtaaac aatgtttatg ctgtaaacgt tattgataat tattaaaaat caaatgtatc
    14341 gcagagaatt tttatcatca attgcaggta taggagctat tggaacttta agttctttta
    14401 aaaacattac tgatgatctg agtaattccg ataagatgcc tgtattgttt atcggacatg
    14461 gctctccaat gaatggcatt gaaaacaatg agttcagcaa taaatggaaa aaattaggtc
    14521 aatcattacc taaaccaaaa gcaattgttg ttgtttctgc acattggctg acaaaaggaa
    14581 cttggattac tgcaatgaac caccctaaaa ctattcatga ttttggtgga tttccgcaag
    14641 cactttataa tgtacaatac aaagcaccgg gcagtcctga attggcacaa gaaacaaaaa
    14701 atattatcaa gtcagtaaat gctgatttag atcatgactg ggggctcgac catggagcat
    14761 ggactgttac aagacgaatg tatcctgatg cgaatattcc tgtgcttcaa atcagtattg
    14821 acattaataa accggcatca tttcattatc aattgggaaa agaacttgct tcacttcgca
    14881 caaaaggagt attgataatt ggaagtggta atatggttca caacctcgga aaaattgcat
    14941 gggataaaat tgatgaagtt ggctttggtt atgattgggc tgttgaaatg catcaacttt
    15001 ttaaatctaa aatttcagac aacgatcatc aggcactgat taactatgca tctcttagca
    15061 agtcagcttt aatggctgtg ccaacaccgg atcattattt tccgttaatg tatgtacttg
    15121 ggctgcaaca aaaaaatgaa aatgcagaat tttttaacga taagttggta gcaggttcaa
    15181 tcaatatgac ttctgtaaaa ttcgggtaaa cgcagattcc aacagttaat attctgaccg
    15241 ttttttcttt ttatcgggaa ctataaaata agtacataat ggtagtccat caacaaacac
    15301 gtccaatgtt ctttttttat tcgattcaaa tacataatcg aaataaacaa gactatcaac
    15361 ctgtgtaaaa gtattttgaa tcttttcaga ttttttacca tcacgaattt caatcttacc
    15421 aatagcaagg ctatccatag atttaaaata aaaacgtttc ttcaaacctt tgtaaacagg
    15481 taatacacca tcagcaggtc ttaatgcata aatgccgtta tcaaaaaaga aatcataaac
    15541 ttcaggatag ttcaaaaaag tattcttgtc aacaatagta tctaaatatt gacgatttcc
    15601 atttaatgga agatgcgtta aaattatctg acgcggatgt aatgcaaaat atctctcagt
    15661 aaagcgtttt gtaaaaatct tatcaccttt ttcaaaatag ccacttgcca tcatcacatc
    15721 catcaagtac catttatcat atagtttaac agtactccag atatgatttg gttttttaat
    15781 ctttgttccc ggtaattgat ttcttcttaa atagccattc accgaaaggc atgtgatatt
    15841 cattttagca cagatagcat tcagaatatt tgcatagcct tcggctagtg cagttttgtt
    15901 cttaaaaaca tcgttagcgt tgagattcgc tttctgcgga tggcgttggt tctttaaatc
    15961 ataagctatg ttttctgtaa tccatctgaa tgcagccctg aaacgaaaat gagggcggtc
    16021 aaattctctg ctgattactt cagctaatgt atcaatgtta acgcattgtt cgggttttaa
    16081 ctttaacaca aactcatcaa cagaaccata atcaagtaca ggaattttaa tcctcttttg
    16141 tgaaaaaagc gatgtggaga gaaacaaaaa aactattgcc gtaaaaaaac ttttatggag
    16201 catatatggt tttggtcaaa aaattttgtc caaattacca caatattctt ttaaagtcaa
    16261 caggctttaa gatgaaccgg aattttttct tcttaaaagc gtaattccaa cgattgaaac
    16321 aataatacat gcaacagcta taagctctgc ctgtgtaatt gcttttccaa atatgtggta
    16381 ctttgtattg actctgatct gttcaattgc caaacgttct acacctgtga atagcaggta
    16441 aacaaagaaa agtattccgg gagtaactat tctagttcgt agcttccaca aaacaaagaa
    16501 taataaaata caggcaattg cttcatataa tggtgtagga aaaacaggag gtatcaattc
    16561 atagcaatgt ttgccaacac atccagcaat aggaacaccc tctccaatta cattgtgtgg
    16621 atagttataa ctccacatcc aatcaggaaa tggaaaccaa gaaggttttg caagtgtatt
    16681 tacaattccc cagtcaccat cgcctgaaaa gtgacaacct aatctgcctg tgccataagt
    16741 cagcattaat gcaggtgcag caacatcaca catcaccaat ggcggcacat ttaatttttt
    16801 attgctatac catatcacag ctaaagcacc acaaatcaat ccgccaaaaa aagttaaacc
    16861 gctgaacgat atcaatgcat caacaggatc tttaagaaag tcatcaatgt tttcaagatt
    16921 atgaaatatt ttggcaccta ttaatcctgc aacggcagca gcaatggtaa tattcattac
    16981 aaactgatga ggatacccaa cttctgtaac agtctgcggc actgccaatt tttgcttttc
    17041 tttttctttg tatttgctcc aaacagaata ggcaccgagt aatatcccgc caatgacatt
    17101 acctcttgtt gataataaaa agccttgtgg atcattaaca aggtcactgt aattcaaaat
    17161 ggcatcaagc aatttaaagc caactaaaaa tcctacaaca cctgaccaaa acaactctgc
    17221 tgttgatgca ggttttcctt taatggtagt aacggtgaaa gactttaata aaccattttg
    17281 ctctttgcgt ttaaactcta agattaaaat atatgcagcc aaaaaaaatg acaatgccaa
    17341 tatgagcccg aatgtttgaa ttggcaatgg aatatttatg cccaataaat catggagtgc
    17401 atcggataga gtagggtaca tgggcagaaa ttattaagcc gtgattgatt ctaaaattgt
    17461 acagttaact tcatggctgt cattgtcggt tgcaccgatt ttacattcaa ttactttatt
    17521 taccatttcg ttattgaaag gtgttgaaac tctcacatag ttttcggaat agcctgacat
    17581 tattccattc ttgttttcgt gttcaaaaag tactttggct gttttacctt cttgtgaccg
    17641 ataaaatatt ccgagttttt tctccgaaag tatgcgcagc atattggttc ttcttttcct
    17701 ttctgcaacc ggaacaacag gcttcatttc aatagcagga gtgtttgctc tttcagaata
    17761 agtaaaaaca tgcaggtaag aaatatcagc ttcgttcaaa tatttataag tctgaagaaa
    17821 atcttcctcc gtttcaccgg gaaatcctgt aatcacatca acaccaatac aggcatcggg
    17881 catcaacttt ttaattttat ttatccgtga tgtgtaatgc gaagtcaggt aacgtctgcg
    17941 catcagtttc agaattttat cagaaccgct ttgtaatgga atatgaaaat gtggcataaa
    18001 tttcttagag cagctaacca attcaatgat ttcttctgtt agcaggtttg gttcaataga
    18061 cgaaattctg aatcggatat cagcatccaa agcatctaat gcagaaacta attgaaaaaa
    18121 ttctgttgca tgctttttct caccaggcag tttaccaaag tcacctaaat taactcctgt
    18181 cagaacaatc tcttttgcac ctctatcaac aatttgtttt acattttcta tgacattttc
    18241 aattgtatta cttctgcttg cgccacgcgc caatggtatg gtgcaaaatg tgcaaccata
    18301 atcacagcca tcctgaactt tcagaaaaac acgagttctg tcaccttcgg agtagctgct
    18361 tgtaaactga tgatgatcgg caatgtcgca agaaaaaacc tgtgcttcgg tttgcttttt
    18421 aagcttttgc aaataatgcg gaatgttgaa cttttcactt gcacctaata caaggctaac
    18481 acccggaatt gtagcaattg catctggttt tagctgtgca taacagccaa taatgatgac
    18541 acatccttcg ggatttgctg ctaaagctct gttgacgtgt tggcggcatt ctctatcagc
    18601 attttcagta acagaacagg tatttatgac ataaacatca gcagtatcag aaaacgaaac
    18661 ttttttgtag cctgcagctt gcaattgacg gccaattgtt gaagtctcag caaagttcaa
    18721 tttacaacct aaggtgtgaa aagctacagt agattccctt gtcattagcg tgcaaaaata
    18781 gcaattaccg ctgaacttta cattccaaaa aactcctttc attttgaact aatcaataag
    18841 agtgtgaatt ttgcagtcta ttcaaaaata atgtcaacaa gcggaacagc atttaattgg
    18901 aaggtgctaa acaggattat gttctatgta aagcctttcc ggtttctttt tgtagccaca
    18961 gtggttatca ctgtgttgct tgctgtattg gcaccattac gaccctggct tacacagcag
    19021 gcacttgata ttgacattgc aaaacacgac atcaaaggtc ttacacgcac tgttgctttc
    19081 atgtttggtg ttctgctact acaatcattg cttcagtttg tctatactac cttaacaaac
    19141 cgacttggac aggaagtaat actttcatta cgtaataatt tgtttaagaa ggtcagcggt
    19201 tttgggctag cttattttga taaaactcct cttggaatta caattacacg attggtaagc
    19261 gatatggaaa ccattgcaga tatattttct gatgggttga ttcttataat aagtgatatg
    19321 cttcaggtag tggtaattat tgctgtaatg ttttacatcc atccacaact tgctgcaatt
    19381 tctttatcta ctattcctgt tctgttgatt gctaccagag tttttcagaa aaatatcaga
    19441 aaaactttta atgatgtgcg catacaggta gctaaactaa acgaatttgt tcaggagcat
    19501 ttagttgcaa tgagaattgt acaacttttt aatcgtgaag aagaggagtt aaataagttt
    19561 catgaaatta atgccaagca tcgcgatgcc aatatccgtt ctatatggta ttacagtttg
    19621 tttttcccag ttgtagaaat tctctctgct gtttcaatag ggcttatagt ttggtatggt
    19681 ggtttaaatg tacttcaaag ttctatcagt ttaggcttac ttgttgcttt tattcaatat
    19741 atcaaccaat tgttcaggcc aattcgtgaa cttgctgata agtttaacac cttacaaatg
    19801 ggcatggtaa gttcagaaag agttttcaag ttgctcgatg aaccatttac agaagttaat
    19861 tcaggcacaa aaagtgccga taatataata ggcaaaattg aattcagaga tgtgtggttt
    19921 gcctataaaa atgaggagtg gattttgcgt ggcttatcct ttatcattcc ttcaggacaa
    19981 tctgtagcga ttgttggatc aacaggcgca gggaaaacta ctattgtaag tttgattaac
    20041 cgcttatatg aaattcaaaa aggacagatt ttaattgacg gtgttgatat cagagattat
    20101 gatttataca gcctgagaaa tgctataggc gttgtccttc aggatgtctt tcttttttct
    20161 gacagtataa agaacaatat tacactctat agagaacaag atgatattgt tattgaagcc
    20221 ttgttgaaac aaacaggagc ctggcatttt gtagaaaagt tacccggtca gctacattac
    20281 aatcccggag aaaggggagg gttaatcagc accggacaga ggcagctatt gtcatttgcc
    20341 agagtgttgg cacatcaacc taaactaatt attttggatg aggcaacaag cagtgttgac
    20401 agtaagctgg agcagctttt ggttaatgca acagaaatgc tgacaaaaaa ccggacttca
    20461 attattattg cccatcgttt atcaactata gaaaaagctg atcgcattat tgttttacat
    20521 aagggagcaa ttgccgaaga cggaaatcat caaaatctat tagaaaggaa aggtatttat
    20581 gctcaccttc atgatgttca atttaaacag ccatcagcat aacttataag agttgatttt
    20641 tgtttctttg cagaatacta tttcataaaa tgaaaacatt ttccggactt atcacggtat
    20701 taatttttta ttctgttcaa gctttttctc aaggttctgc gttgatgaaa gaagatgccg
    20761 tacaggttaa taaatttaat gatggtgttt attttacatt aacagacctg caacataaca
    20821 caccaactgt tactaaagat catctcataa aatcatatta cgaccaatct gatttcagta
    20881 tcggccagtg ggcaaccaca caaaacttat tttatactgc aaaagatgaa cataaattga
    20941 aagtcaaccg agatagctta tggggttatg tagagaatgg aacgccatac atttatctta
    21001 acgacaggtt tcatcggttt tcaacggcag gagccatttc agtatttact gaaagttatc
    21061 ctaatatcaa agccggtctg gcacctgtag taacagaagc aagatcttca tcagttatca
    21121 atctgtttat actttccaca ggaaaaatag tggaatttac acctgaaaat gttgctgatg
    21181 caattgaagg caatgaacaa ctttataagg agtttaatgc attaaaatcg tggaaacaga
    21241 aacgcaaaaa aatgtatagg tttgtagagc ggtataatga aacagaagcc ttttaaacct
    21301 taattaaaat ttattttatg aaaaaattga agtattttat ttttgtatta ctcattttaa
    21361 tttcctttta caacttaaca agttgttcac agaaaaagga ggcgatatct gatttacccg
    21421 gttttgtaca aattaaaaac aatcaatttg ttcttaatgg aaatccatat ttcccattgg
    21481 cagtcaatta tagagtagca cttcaaacta atggaaaaca actttggcca tcagcatata
    21541 aaggatatga tgatggtaat cgctaccggt tcacaaacag agattcaagt ctggaagaat
    21601 tgaataatga ctttcagctt atagctgata tgggatttaa ttcgatcaga attgttggaa
    21661 taggtgaaca atattctgtt gcaaagaact cagacgaatt atacattaga gctgacatag
    21721 gtaatgaaaa ggacacgatg ctacttttta attcacagga aacttacaac caatatttca
    21781 atgcattaaa cgacatgctt caggcagcaa ataaagcagg cctaagagta gttttgttat
    21841 caagactttt tcatgaagta ccagcaacag aaaaacattg gggaagaata atgaaacatt
    21901 ttcagaatga tactacaatt ctggcgtatg atttttttaa tgagcctttg tatttcgatt
    21961 cattggagag aaataaaacg gagccatact atattactaa agcctggtat gaactggcta
    22021 aggaaaatgc acctcatcag ttaactacaa ttggattgac cggcattcgt gaagtatttg
    22081 aatgggatcc taatacatta aatgtagatt tcatttcata tcatccttat gaatatgaac
    22141 ctgaacaagt tcgtaatgaa atttactggt atggaaatta tgttaagaaa ccttggattc
    22201 ttggagaaac ttcattgccg gccgataatg attctgttcc atatagtatt cagaaaaatt
    22261 ttgcggaagc tacttttaat caaacttgca attgtggtgg cataggctat acatggtggc
    22321 aatataaaga tgttgattgg tttgattttc attccaattt tatgggtgtt gtcaacagaa
    22381 aaggagctac attgacttca tcaggcaagt ttgttatagg tacacccaaa tctatgatag
    22441 actttataaa acatgcaaaa cctttaaaag actcaactaa atgtttaaaa ttggaaaact
    22501 actataatta ctccaatgca aatgtttgtc gcttaattgg gaaattagtt ggaccatctg
    22561 gagagcctat agcgggtggt gttataatgg catggaatca atattggtca agttctttcc
    22621 atacagtaac aaaagaagat gggacttttg aattgaaggg aagttttccc ttttatcatt
    22681 ggatggcatc tgctaccatg atgaccatga tacgaggtga tgttttaccg gatacagcaa
    22741 aaatcaatgt tcatgagatt ccaacattgg atatgggtat tttgaaagtc gcaaaattga
    22801 aattggatta attacggttt tcaattttat acatttttgc tgcagctctt tctaataaca
    22861 aagttgtttt gtagtttgct tctatatact ctttttcata ttcctgcgat gcaattgcta
    22921 catcaccagg agacaaatct ttataatatt tgtaataaca agtaatgttc tgctttgata
    22981 gttttgttat atagaaatta atttgaggtc catatccatt atcccagcaa attacacaag
    23041 tcttgacctc cgagggtaga tttttaagaa taaatggaat gctttcatta ttgtaatcat
    23101 acatttttgg gaaatagact ttatcaataa tagcacgata agggaataaa aaaataaaaa
    23161 acagaattat atatggtgca atattaaatt ttaaccgttc cgcaaattgc gggatgtgtt
    23221 taactaataa aaataattcg taaatactaa tactacataa aattgccata aaaggaaaca
    23281 tgggcaaatc gtaccattct aattttgttt gagcacatga gattaccaaa aaataaaata
    23341 gggttaatat catgcaaaat attgataact tcttaactgt cgtatttttg tttaataatc
    23401 ctataaaaac accaataaac attggtaagt accaatagtt gaattgttta ttaagaatat
    23461 tatcataata aaatgtaaat ccatgatcat ggctttcaat tgtttttaaa tatcttccgc
    23521 caagctcatt ttcaattaca gctttcaaat atcccggatt ttgtgtctcc ctaataaaat
    23581 agtaaccagc aacaaccgct atacaaaata tgctgtaaaa cacagttgat ttaaattgaa
    23641 atttaattcc ggaatcttta tgattgagga atcctattag gagtaatcct ggcaaaaata
    23701 ttaatggttg aattcctttt gttaatatgg caagtgttaa atacaaaaaa aacaacttaa
    23761 aatgaattat ttttcctatg gataaataat tgtaaaaact aaaactaaaa agtgtgatga
    23821 aaagaattaa taaggcatca taatctcctg ttcgtgttcc atgatatccc acataccctt
    23881 gagatgtaac caatacaagg gcagcaataa acccaaacca gtaattttta gtgtggcgtt
    23941 ttaaaaagaa aaataatgca aaacaagtaa ttaagcccgc tattgcagat ggaagcctta
    24001 ttgccaactc atttattcct atgagtttta ttaatccggc ctgcatccaa attaaaagag
    24061 gcggttttgt attccataag tcaggttttc cttcataatg agtaactata atatcattgg
    24121 taatactcat ttcaatagca ttagatgcca atctagcttc atcccaaata taaatcggca
    24181 ttctttgcag atgcagaaat aatggaaagt agagcaagat gcccaagaat aaaattaata
    24241 agacatcaag atgacgtttt atcatttaat ttttaaaatt actttaaaat tttaatttca
    24301 aatgttgatt gctcaatgtt aatttcatca gttaatcgta cctctttagt ttctttatca
    24361 acagttgcat atttattgta aattttaata acgctccttt gttgctgata gggttcaata
    24421 tttaccaagt cagaatttaa aaatgaattt ttgtaaccta acaatcgttt gttatatggg
    24481 tcaactttta agtaaacctg atctaaggaa gaaatcaatg tcttacccga attaaatttc
    24541 aaggcaagaa aagtttccca tgactgaggt atttttctat tcgccattat taaatcatta
    24601 tagtttttat ccaagcacca atacaacgag tcgtttgtca taatttgcat ggtatctaaa
    24661 taaaaaccac caacttcaaa atcattcatt atctgaatat ctgaatctga aattttattg
    24721 tcattacacc atgtctttaa gctactactg tcaggatatt caaaaaattg cagaaagttg
    24781 tatggattaa atcctgaata tccattaaca catttaatat gcaaatcctg acatgctaac
    24841 attatactta atgtttgtga aacagcagta aaattcttgt tctcagcttt tgttaatgcc
    24901 aaatcaacag ggaaaaatgc aattgcttta tattttgaat tatattgttc agaaattata
    24961 cttttaaaat attgaacatt tgcctgtgct tctgatttcg aaatactttt atgaggttct
    25021 ggtataaagt aattatcaaa aagaactagg attggtaaca agcaagcaaa taaaacttta
    25081 agcctaatat gctttattag aaagtgtaaa gaaaatgcag taagtaaaac gaaaagtaat
    25141 atttgtatat tcattattct atcaaccgat ctcattgatg aaaatccggg aatcaaataa
    25201 ataattttat ataaactgta attttttata ttcagacaga agataagaga taaaacaaaa
    25261 gttattataa ttaacaaaag tattttagaa ttttcgctat tctttttgag agctataaaa
    25321 acaaacggac ttgcaacaac accaagccat ggtattatac cgggaaataa taagtgattc
    25381 caccaattaa taaacttaaa ggctgtaaat ggatataaaa aattccatgt cactgatgct
    25441 ggagatgtaa aaaaatatga tcttaatctt ggcaaagagt tgagtgaata ctcataggat
    25501 cttgtcccca cttgatctgc actgtgaata tatggtagaa ataggggcaa taaaacaatg
    25561 attgttccaa taattgataa agtatagttc aataaagcct ttttatttaa ttcgaatggt
    25621 agttttaaat taacaataaa tgctgcaata aaaagaaaca taataaagta agctaagaaa
    25681 aaacctaaat agattccaca ataaaactga tatgctaaac ccaataataa aaaagtaaaa
    25741 tatctacttt tcaaggtttg aataaacctg attgaccaat agataactaa gggtgcaata
    25801 aatctaggaa aaacctgaat atgaaaaatt tgcccatggt tgtacaatcc aaatccataa
    25861 ataaatgcac agcttgctgc aatgagatta ttaatgccga atttttttag aacgtaaaaa
    25921 catgaccaat agttcaaaag gaaaagcaca atgatccaaa gttgaaaaga tgtttcacga
    25981 tcaacattaa aaactcggaa gatggcatat ataggcattg taccaattaa attatccgat
    26041 agtgcaatta cattcttata aggatacatt gaagaggcat cccaaaaccc atgaatctga
    26101 tcagttaaat attgatatcc atgctcaaga atataatagt taaaacgggc atcacctaaa
    26161 tcaccgggaa tgcgccccat atcccatcca aaatagggca tcactccaaa ataaatccct
    26221 gctaacatca taaaaacagg gattaattta tttaatgaaa ttttcaggct ctcggtcttc
    26281 atctgtttat tagctaaaat taaaataaaa aattaatggc ttaatccata tctaacaata
    26341 caccacaatg ctctgaagcc atctttccat ccaatctttt ttccttcggc ataggtccgt
    26401 ccatagtacg aaattccaac ttcataaata cgtacattag gtattcttgc aattttagca
    26461 gttacttccg gttcaaaacc aaatcttttt tctttgagct ttagtgattg taccattttt
    26521 gtattaaaca ttttatagca agtttccatg tctgttaggt tcaaattggt gaacatattg
    26581 ctacaaaatg tcaagaattt atttccgatg gaatgccaga agaataaaat tctatgtggc
    26641 ttaccaccta aaaaacgtga tccataaact acatctgcaa aacctgtaag aattggtttt
    26701 aacaaaatat tgtactcttc cggatcatat tcaagatcgg catcctgaat gattaaatat
    26761 tctcctgttg ccaattcaat gcccttatgc agtgctgcgc ctttacccat attaacaggc
    26821 tgattaaaaa gtctcatgtc agcctctggg tgatctgtaa tatatttctc aattacctct
    26881 ttggtagtat cttttgaaca gtcgtttaca agaataattt ctttgctgat gttattagtc
    26941 agtttaactg ctaaaacttt gtcaagaatt aaatgaatgg ttcgcgcttc gttatatgca
    27001 ggaataatga tggaaagttt ctgtatcatt ttgctttaat gtcaattacc agggattttt
    27061 gaaatcatcg gtattaaact ttttatttcg gttgctaaaa taggttaatt catctgattt
    27121 tttttgccga tagaattgat tgccctttcc aaacattttg cgtatcaatg ctgttggcca
    27181 aaatataccg aagtaaataa taaaaagtgc aatttttgat attaatcttc caatagaagt
    27241 agttactaaa gaccaaacct tataaataat ttttgcaagc ggaaaaacaa ataataaaat
    27301 aatcagtaat aaagccgcaa ctactacaag ccaatccaag ctgttaaaat tttctgcttt
    27361 atacttctga taaagaaaaa ccattaataa aatgaggcat attgaagaaa aagtttgcca
    27421 aatttgctta ttcaacataa ttaaaacagg gtgtaaataa acggtgcaaa tgcaggattg
    27481 cttccggcaa ttatcaatac actcattaat agaatgacaa taattaatgg tgccagccag
    27541 tatttttttc gctgaattat aaatctgaaa agatctgata gtaattccat tgacttgagt
    27601 gttacaaata tacaatattt aaatttcttt tgttatatac agtgtaaggc actattgtgg
    27661 caaatgaaat gctatttttg cagactgaaa aaaatatccg gaatgttgct taaacaggaa
    27721 attgagaaac gaagaacttt tgcaataata tcgcaccccg atgcaggtaa aacaacttta
    27781 acagagaagt tgctgttgtt tggtggtgct atacaggttg caggtgcagt taaatcgaac
    27841 aaaataaaga agactgctgc cagcgatttt atggaaattg aaaaacaaag aggtatttct
    27901 gttgcaacat cagtgatgac tttcttttat aaaaactgcc agataaattt attagacact
    27961 ccgggccaca aggactttgc tgaggataca tacagaacat taaccgctgt ggatagtgta
    28021 attcttgtaa ttgactctgt gaaaggggta gagccacaaa cagaaaagtt gatggaggta
    28081 tgccgcatgc gaaatactcc agtaattatt ttcatcaata agatggatcg tgaaggtcag
    28141 aatccatttg atttattaga tgagttggaa gaaaaacttt ttatccatac acgtccacta
    28201 agttggccca tcagtagcgg atctactttt cagggcgttt ataacttaca caataaaagt
    28261 ctgatgcttt ttagagcaaa ccaaaaggct tctgacagtg atgttcttcc gattaaagat
    28321 atgcaagacc cactacttga caaacagctt gggcgctatg ttgatcagct tcgtgaagat
    28381 gttgagttga tagaaggtgt ttatgagcca ttcaacaaac aactctatag tgaaggctta
    28441 cttgcaccgg tattttttgg tagtggtatt aataattttg gtgttaaaga actgttagat
    28501 acttttattg aaattgcgcc tgagcctaaa tcccgacaga cagaccaacg tacagtgaca
    28561 ccagaagaaa gtaagttctc cgggtttgtg tttaagatac atgccaatat tgaccctcgt
    28621 catcgtgaca gaatagcttt tttaagagta tgttccggca tgtttgaacg tggtaagttt
    28681 tatcatcatg tcaggttgaa taaagattta cgattcaaca atccggcaac ttttatggct
    28741 caagaaaaaa gtattgtgga gcaagctttt ccgggtgatg ttataggact ttatgataca
    28801 ggaaacttta aaattggtga tacacttaca gagggggagg aactgatgtt taaaggtatt
    28861 ccgagttttt caccagaaat ttttaaagaa cttatcaata ccgaccccat gaaaaccaag
    28921 caacttgaaa aaggaattga acagctaacg gatgagggag tggcgcagtt gtttattcag
    28981 aatggtggta acagaaaaat tattggtaca gttggcgaac ttcagtttga agttattcaa
    29041 tacagacttc agaacgaata tggagcatct tgtaaattta taacacagaa tatatcaaag
    29101 gcctgttggc tcacttcaaa agacaagaat aagctagatg aattcatgcg ctttaaaatg
    29161 aacaacattg cattcgataa agatcaaaat cctgtttatc ttgccgactc acaatggatg
    29221 ttagaccaga tgataaagaa ttatcctgaa gtacaatttc attttaccag cgaatttaaa
    29281 atggaagttg ctgtttaatt ctgcctaagt agtcctatca gacacataag tgctttttta
    29341 ttctgagaat taaaatcatg taaagttgca attggatatg caattgagag ttttataaaa
    29401 ttcattttcc ggcatctcgg ttttacatcc ttgatttcag ccaatggaat gtagttgtct
    29461 ttatgtaaat catagcgcca aggtttttca ttgatacaca acaatacatt ttctgattta
    29521 gaggattgca ttttatcata gtcaagcata ttcaaaaatt tgccacttaa atgaagtgtc
    29581 acactgtaat gattagccca ccagaacaaa aagcgaattg cgaaagtctc ctccttatta
    29641 aaccatcccg gagcatcaca atttatccaa ggtaaccctt caagattttc tcccctgtag
    29701 attttgctgc gatttataat tgtagattct attttaaaat cactttccag tttttcttta
    29761 gccatagtgc ctaactctgc aaagttactg tataccttat ctgtgatggc tcttttaata
    29821 ttaaaaaagt cagcagagat ggcatgctgt atttcttctg atgaaaacat aagtccgtga
    29881 agatagcaat tactttttcg ttttatttgt gttgtaaatt aattgttgaa tgaaacttac
    29941 tataatcagt gccagtgtac gagaagggcg caaaagccat cgtgcagcat tattttttaa
    30001 aaattttctg atcaaaaaat atcaggcaga tgttagcatt gccgatctga atgaacttaa
    30061 cttccctata tttgaagagc gacttagatt tcagaaaaac aagtctcctg aagttgttca
    30121 atttgcagaa aacattaaac agtctgatgg tgttttaata attacgcctg agtataatgg
    30181 aggctatccg gcatcattaa aaaatgtaat agacctgatg tatgatgaat ggcgcagaaa
    30241 gcctatagcc attgcaactg tttctgatgg aatgtttgga ggaatgcaag taattacttc
    30301 cttgcagttt attttatgga aaataagagc atggactgta cctgctatgt ttccaatgcc
    30361 gcagatagat aaaaattttg atgttgacgg aacaccggtt gatgttgcag gcacagaaaa
    30421 aagagctaca gtattttgtg atgaatggat gtggtgtatt gaagcaaaca gaagaatgca
    30481 gttgaatcat taaatgaatt taaaccaaag taattttaat ttgtttattc ctgataatat
    30541 tcaaaaaatg tattacatga caagtttaat cgcaataatt ttgcttacag gttgtcagaa
    30601 tcaggcacag tcactacggg aaatttctaa ttctacaaat atgcaaagca acatagccac
    30661 agacacagca actttcggaa caggatgctt ttggtgtaca gaagccgttt ttgaatctct
    30721 tgaaggagta atctcggcaa cttcaggtta ttccggtggc aatgttgcag acccaaccta
    30781 tgagcaggtg tgcagtggca acacaggtca tgcagaatgt attcaggtag tttatgactc
    30841 taaaaaaatt tcttatgatg accttttgca agtattttgg gaaagccatg accctacaac
    30901 attaaacaaa caaggcgctg atgtgggtac acaataccgt tctgtaatat tttatcataa
    30961 cccacagcaa aagcagttag cagaatcgta caagcaaaaa ctgaacaacg aaaatacttt
    31021 tggcaagcct gttgtgactg agataacagc agcaactaca ttttatcctg ctgaaaatta
    31081 tcatcaggaa tattttaaac tgaatggtca cgcaccatac tgtcagtttg ttattcaacc
    31141 taaggttgaa aaaatcagaa agatttttag ttcaaaaatt aaaaaataac tgaagctatt
    31201 tctttttcaa taatgctttg cgtagttgtg cgcttgtttt acagtcgcca tcatgactcc
    31261 aacccggagg accgagtaag tacataattt tatttttaac agatgttttc tttttcaaat
    31321 catgtgcaat agcctgccat tcgtgaaata caatttttcc cggattgtta gaatgtaaat
    31381 tatttgtcag accatactta atgggttcat tttccaattc cctctgaaaa gtaccaaaca
    31441 atttatccca gattatcaaa accattccca tgtttctgtc aagataacaa acattgcttg
    31501 catggtgaac tctgtgatgt gatggagtta caagaaaata ttcaagccat cctaatttgc
    31561 caacggtttg cgtgtgaacc aatattccaa aaatctgtgt cgcgctataa ataaacataa
    31621 tgtcaatagc cttaaagcca acgatgctaa gtggaataaa ataaatgaac cgataaagtg
    31681 gttgcaaaac agaagaacgg aatccaacag taaggttgaa ctcttcgctg ctgtgatgag
    31741 tgacatgtac agcccagaat aggcgacaaa aatggtctat ccgatgcagc caataataca
    31801 taaaatcttc tgcaagaaat aaagctaacc agtaaatcca tgcagtaggg taggagttaa
    31861 taattctgaa atcgaaaaaa taattcagaa ttaacagact tgcagtacgc attataatat
    31921 cgagtcccat attcagcaac atgagataga gattgacaag agttcctttt tttgaataga
    31981 gttgtaaatg atgaaatgca gaaaaaaata tttcagcacc aataactata atgtaaatag
    32041 gtgttgaaat cagaatcagc aacgactctc tgaaatgttc catataaaat actgaagtaa
    32101 aattttattc agcagcaggc ttctttttac gcggtgctgc cggctttttc gcagcagatt
    32161 tcctattggg cttaatttct tctgttaaag tagattcaaa gtcagaaaat gcattcatat
    32221 aaaaaatata agcatcgtaa ggtgagttat aagggctgaa atatttatgt acatcgccat
    32281 cactccagaa cttagtactc atataataaa aatgatcgct ggtttgtagc ttaccccaaa
    32341 cgtcaattaa atctttgttt cctgacccaa gcaccctgtc acttattgaa taaattttat
    32401 tgagcgcttc ctgttgcata ttatttccca gccatgctga aagatcgcgt tcagtatctg
    32461 cccaactgat aaactcatgt gcatcatata caccgcgagg cgtataagca tctacaacct
    32521 gtgatggtgt cataaagcca aagtcaggat gctttaatat ttcacgaggc atgtgatcca
    32581 taaaatcaaa aatgcctgtg ctttcccact gatgctcgcc aaaagtttca taatccataa
    32641 acaggtttat cacatcacca ctacctgcaa ctttgtgtac ccaacttgca aatgtatcgg
    32701 catgtagtgg ccattcgctc cagtttttgt cagagaatct aaaagcaatg tcatcgctca
    32761 aacgataatt tttaagtaat gcttttattg attttgttcc tgttggtttc aataaatgat
    32821 tagggtgtct gtctttcaat aaacgatcaa caccttcgca caaaatccct ttaaagccca
    32881 ttttttcaat aaaggcagca agctcattgt tgtaaattaa ttcggtattt ctgaaaacag
    32941 ttggcttctg tttaaagtaa tgttcaattt tctgatcatg ctcctttatt tgtctctcaa
    33001 actcttcttt gttatataaa aaactgagtg aatgaaaata tgtttcagag aggaattcta
    33061 cacaacctgt tttagacaaa tcaacaaatg actgaagaac atccggtctg aacaattcca
    33121 tctgttctat cactgttcca cttagtgaat agcttattct gaatttacct ttatgacgct
    33181 taatcagctc aagcattttt ttattggcag gaaggtaaca tttctcagat accttgtcca
    33241 taatccattt atttttttcg ccatcttcat aaaaatggtc tttaccaatg tcgaaaacac
    33301 tgtaatgttt tattctgaag ggctgatgca cctgaaagta aaagcaaacc gatggcataa
    33361 atttgttttt aaaaatggtc gttgtgccaa aattaaactt ttgcctgaaa caaaagaaaa
    33421 tatcctgaaa ctgtcatcat aaatttatga ttaaagggat ggtacataaa tcaaaaagcc
    33481 gctaaaagcg gctttttgat tttattgatg aataagcaga ttaattgttt tctgcttcgt
    33541 tcttcttcat tttgtcttta agtgcagaaa gaacgctcaa atcacccaag gttgtttttt
    33601 cctgagaatc tttgattttc tttactgact taaatgcatc actttcatct ttagctttct
    33661 cattgtcgcg cgccatcttt tcagatgctt ttgcttcttc gaacaagcgg ctgtgagaaa
    33721 caacaatctt cttgttctct ttgctgaatt cgattacttt aaaatcaagt gtttcttcag
    33781 ctttagcagg agtaccgttt tctttcatca gatgacgata tggagcaaaa ccttcaacgc
    33841 caaatgatgg gaatatgata gtagcacctt tatcggttac ttttccaaca acacctgaat
    33901 gaacagtgtc aacacggaaa attgtttcgt aagtatccca aggattttct tctaactgct
    33961 tatgtccaag acttaatctg cggtgctctg aatcaacttc aagaactaca acttcaatgt
    34021 tttcgccaac cttggtaaac tcagcaggat gcttaatttt cttagcccat gaaaggtcag
    34081 agatatgaat taaaccatca ataccttctt ctaactcaac aaaaacacca aagtttgtca
    34141 ggctgcgaac ttttgcagta tgctttgagc caacaggata tttctccata atgttaaccc
    34201 atggatcagg cataagttgc tttaagccaa gagacatttt acgctcttcg cggtcaagag
    34261 taaggattac agtttctatt tcatcaccaa ctttaagaaa gtcttgcggg ctgcgtaaat
    34321 gctgtgacca tgacatttcc gaaacgtgaa ttaatccttc aacacctgga attacttcaa
    34381 tgaaagcacc atagtcagtc aacacaacaa cttttccttt gattttgtcg ccaacctgaa
    34441 tgttttggtc aagtgcttcc catggctgag gtgtaagctg tttcaagcca agagcaatac
    34501 gttttttatc ctcatcaaaa tcaaggatta caacattaat tttatcatct aacttcaaca
    34561 cttcatcagg atggttgatt cgtccccatg aaatatcggt aatgtgcaat aaaccgtcaa
    34621 cgccaccaag gtccataaat acaccataag acgtgatgtt tttaactgta ccttcaagaa
    34681 cctgtccttt ttcaagttta gacatgattt ctgacttttg gctttcaagt tcctcttcga
    34741 taagtacttt gtgagaaaca actacgttct taaactcatg gttgatttta accactttaa
    34801 attccatagt ttttccaaca aacacatcat agtcgcggat tggtttcaca tcaatttgcg
    34861 aacccggtaa aaatgcttca ataccaaata cgtcaacgat aagtccgcct ttagtacggc
    34921 atttaacata accctgaata atttcatctt tttcaagggc ttcattaaca cgctcccatg
    34981 atttaagagc acgtgctttt ttatgtgata atatcagttg cccgttttta tcttcacggg
    35041 tttctacata cacttcaatt tcatcgccca cttttaagtc aggcatgtga cggaattcgc
    35101 tcagtggaac cattccatct gatttgaatc cgatgtttac caccacttct ttatcagaaa
    35161 tgctgatgat agaacccatt tttacctcat gttctgtaag tgtgttaagg gtttgatcgt
    35221 acatggcctc catacgttgg cggtcatctg atttgtaaat gtcggctgtt ttgccaacgt
    35281 tgtcccaatc gaaatctgtc ggtggaacgt tgtgttttac ttcactcatt aattgttttg
    35341 ttttttaata attgaaattg ttagacttta tttattgttt atgaatatac cttttaaaac
    35401 gggcgcgcaa agataggtat ttgctttgat aaataaaact aatatgcctg ataattattc
    35461 agttccggtt gttgaaggtt ctgcagcctg aaattcccaa aaaaccaata aaaaagggct
    35521 aaaccaatcg gtttaaccct taatctttat gcttttagag caggctcaat taatctgata
    35581 gtaaatatct gtctgccact tggcggtatc tttctcaaca gaagggtctg taatgtaaaa
    35641 ttcaagaggt gcatcagaga tttttatgct gttatcctta gcgtagttta cgagtgcttc
    35701 atgagctttg ttggaattat cataattgcc aaaatactga gtcataaggt attttcctgt
    35761 tttaacttcg ccagcaacaa tatttccggc tgattttcct gctgcagcca atggcataca
    35821 aacatcatat tcaaaaacat tattttccca cttgtaataa attgcaaaag gatgaacagt
    35881 cttactcatt tctaatttct gctttttcat ttctgtatct atcaaaccat aacttgagcc
    35941 aataaaatag ctgattgaat ctaagcctga agtacatctg gtagctaaat atggaataga
    36001 cgaaggcatt tcaacttgtt gaatattaaa ctcaaatccg ggttgtgcag gcatgttttc
    36061 tgctaactct ttaattgctt tcaagccatt gtctgtcatg tcaatcatca tacctttaaa
    36121 cataagcatc atatacttat gtagaggatt ccagccaact tcactttcaa aataagtagt
    36181 aagtttaacg ctgtcaccgg taggttgaat attaaaacca tttacagcat ttccttcacc
    36241 ttcgaaaagg agctctgatt ctacagactg atcttgcaca ctttttttaa tctgcatgga
    36301 cccgtttcca acatttttat tagctgatgt ccactgatat tgagccccaa caccggcttc
    36361 gatattgtta tacgtgactt tcattgtcgt atccatttta agccaggtat tccatttgct
    36421 ccagtttttg agattgttaa tctgattaaa cacaacagaa gcaggagctt ttatataaat
    36481 atcgcgttca atcttatagt tgttggagat gaatagggag ccaacacata acaatccgat
    36541 gatgatgaca aatccaatga gtatcttctt tagcataaca atttgattta gtggttttac
    36601 gagggtaaaa gtagtgtaaa ttatcttaca aaatattcat atacaaacta aaaaaaacga
    36661 aaaaccctat aaataatagg ggttaccata atattccaca aatgcaatat attatgttaa
    36721 ataaataata tgtaaatttt taacctctgt gaagaaagca tggtctttac ggaaatcagc
    36781 aattgttaat tatcttattt aaagtaatga ttctatcaat taaccataat aatttcatta
    36841 tcaacgtatt aatcaactta tacgattaat aattaaacat tgaaaacatt actttaatta
    36901 gatgtctgat attttattgc atcggtgtta taaataatac ttttttactt gcaatctaat
    36961 gcgctcgtaa aaatcatcta aaaacaaatt ttcctgatag gggactttta agtttattaa
    37021 tctggaactt ttttactcac ttcagaaata tagaattatt cagtttttca aatttaaatt
    37081 tttgcaacag gtttactaaa atgaataaga cattgctctg aaatttttaa atgtacaaaa
    37141 ccggttaaga atggcttatg tatcatacaa atttgaacta tattcttagc cataacattg
    37201 tgcggattcc taatgattct taccctggcc gaccggcaat tcaatcttat aattaatatt
    37261 atgttaaata gtaattattt gaatattacc tgctaccagc aattctgttt aacccggcct
    37321 tagccttttg gtctaaactg ttctggtact catggtctcg taaactaagg cacttcttat
    37381 agtatttttc agctaaaaca taatccttct ctttctcata catctgtgct agaaaattac
    37441 aactggcagc tgcataataa aatttcaaat cctgcccgtg atcaatagtt ttcagataaa
    37501 attctttggc ccgatcagtc atttccaact tgtccaacac ccttgccagc ctatatttat
    37561 actccaactg atctttagct gttttagctt cgtttgaacc tttagccaac aattttaacg
    37621 cttcctgata atagcctcca tcaaataata gcctgatttt taagatataa gtatttggct
    37681 cttgatgcgt aattaattca ttttgcgcct gtttatcctc atcagtaaaa tcattaccta
    37741 cgttttttac ttcagtaatc attgcatcat atccggcctt gtcattttgt aatagcttta
    37801 tccatgccat tttttgatat gcagccttta taaagctatt tcctttatgc aatttaacat
    37861 aattaataaa atttgagaat gatgaaaagt ctaatttatc aagcttagac attcctaaaa
    37921 gaaattgaaa gtaatgtatc ggataaacct catcatctgc atgataattt tctatcagtg
    37981 gaattatctg cgcactacgc cctgtagaat aataaatgtt agtagaaaaa aacaagtcca
    38041 aaggctcgtt tcccaatttc aatcctgaca aaaacttgat tacccactca ggatcaggtt
    38101 tcagttgatt acgtgtaaaa atataagtaa aggcaatttc cttattcata aaatgaaaag
    38161 catcactgtc ttctcttgtc ttcataaacg attgctccaa ctcttttatt ccattggaaa
    38221 cgctaccatc cataccggca attttcacta accattgata gttttcaggc actgcaccaa
    38281 taaatgcatg cagcacacca agactcttta gattcaactc aaattcagga aacttttttt
    38341 gattctctgt aagcaaacta tatgctttct ttacttgcaa agctgcagtc agatattcct
    38401 tgaatttcag tttcgacaat gctgtcatca gataacattc tgcaagacaa aaatttcgta
    38461 atggcgaaag cattggcatt gccttaatct gtcctgttat aatttcatgg ttatgtttta
    38521 gctcattaaa agtagcctgc tcctccccta taaatgtttt tatgaataaa tgataatgat
    38581 aaagatatcc cggaataaga ttgtcgggat gttctttctt ctctgcatca agactatcta
    38641 aagcctgatt aaaacgcaat tcaaaaataa gctgataggc attcttacat ctttcattat
    38701 agtcaagata gggtgctgac catacactta atggcatact aaaaaacaaa aaatataaaa
    38761 ttatataccg aagcctcatc agtcttaaaa aactatcgtg ttgtgcaata atacaaagac
    38821 caaacgataa aatgaatcaa aaatatattg atactagcaa tgaggctatg ttgaatacag
    38881 tattgaatga acaaaaaaac tctctataat ttttagtttt gtaaaatgat tagaggtaaa
    38941 atcataaatc caatacgtca gcttgcagag caaggcaaat tagccggcct tttgattatt
    39001 gttctcacgc tggtgtcatt gcttctaagt aatttatcgg caacatcaga atatgcaggc
    39061 ctatggcata gccatataca tttattgggt tacgatgtaa gtgtgctgca cataattaat
    39121 gacggattaa tggtggtatt ttttctgatg gttggcatgg aaataaaacg agagctgtat
    39181 gtgggcgaac ttgccaatag gcgcaatgca atgttgccgg tcatagcagc ttttggtgga
    39241 atgcttgctc ccggaattat ctatgcagca tttaacatca gtgatactca tctgcttcat
    39301 ggttgggcaa ttcccactgc aacagacatt gcattttctc ttggtgtact atctatgctg
    39361 ggtaacagag tgccgtttgc acttaaagtt tttttaactg cattagcaat tattgatgac
    39421 cttggcgcta ttatcatcat tgcattattc tacaccgaac ccgagaattt tcatctgtct
    39481 tttttagtca gtagtttact cattgttggc gcattaatgt ttctttcaaa caaaggtttc
    39541 tttaatttat ttctttggat tattgcaggt attgtagtct ggtattttat gctgctttca
    39601 ggagttcata caacaattgc aggtgtactg atagcattta caattccatt aaacaaactc
    39661 acatcattcg aaaacaaact tcacctgcct gttaactatt ttattctccc tgtttttgca
    39721 ttagctaata ctgctattgt tttatctata gataatgttt ccggattgtt ttctactgtg
    39781 ggattagggg taagtttggg tttgctaatt ggaaagccat taggcattac attagcttca
    39841 tggattgcag taaaaaacaa atggtgttca ctgccaggaa aaattaattt aaagttgatt
    39901 tttggagcag gtttaacagc aggcataggt tttacgatgt ctatttttat tgcttcgctt
    39961 gcttttaaag aacccttact actcgacact gcaaaaattg caattattgc cggttctgtt
    40021 ttatcgggtt tggcaggtgt tttattttta aaatcaaatt tgaaagcaaa agcatgattt
    40081 atttttaatc atccttcgga tagcgcttgc gctgtttgtt caatgggtct tcgtgaaaaa
    40141 aatatctgat atctacagta agataatttc ctgaaatatc agattcaaac actttataat
    40201 ttctgcctgt tattaaagtt cttgagcgat aaataaaact ataaggacgg tgaaaagaaa
    40261 ctccaagata aaaataaccg gatttttctg ttctatattc tgcacctaca ttggcaatga
    40321 tggcaggttt tatccagctt tttcttaatc cttcactttc aaaataatcg tcacctatat
    40381 aaatgtctga aggataaaaa tcgaatgaat tgcctaacga tgcatcaata tacatttttt
    40441 gtccaagtct gatatatacc aatgcagaaa taggtatttc atatccaata aatttaaaat
    40501 ttcgtttatg gcttaacgct gaatcagaaa tctgaatctg ataatttctt cgtgcataat
    40561 ttattcccgt ctcaatagaa agtcgttctg taaaaccacg tctgataacc atgcctgcac
    40621 aatagcctga attaggttga atggttactt ctgccgttcc ttgcgttgct gactgtgcgc
    40681 cagttctgaa aaagtcatta ttaaagatcg gtttaaactg aaaaccgatt gttgcaacac
    40741 ctttttgtga aaatacattt attgataaca tacaaaacac tgcaataaat acagatttat
    40801 taatacttat tgcaaattgg gtcttgaaaa ataggttgaa aaatttcatt cctgaatgta
    40861 agttaataat tcaatttttc gcatttgcat tttttctttt tataaaaagg ttcgcaaaaa
    40921 tgcggatcat ctttatcggg ttttgtcaga tattcaaata agtctctttt tgtattataa
    40981 cgatattccg aaccgtcaat cataaacgtt gcatcagtat tggttctgtt ataaaaatcg
    41041 ctaatgtcgt cttcaatagt gtgaatgctc ccttttgttt tataggctaa gttaatatac
    41101 tgcggattga tgccactgta ggtgccacag aggacaacct ttacaggaac ttttatgtct
    41161 tttaccaaac aataatcgcg catacaggaa ttgttatcag caattaaaat caggttttta
    41221 aaatcgggat acttctgaat gccttttaat attgcctcta catcattttc ttcggggtcg
    41281 ccaccattac ctttggcttt aaccttacta agcaagccaa ttattttgtt gatgttttct
    41341 gctttttcaa aataaacacc tctggtcttt ccaatttttt ttcgtttttc catattacca
    41401 tcattaaaga aaacgaaata ttttatgcct gattgtttaa gattcaatga gtgccaaagt
    41461 acagcttgtg caccataagg atacatgctg cctgtccagt ccataatcac caagcaattg
    41521 tcccattcgg gatggcgcga aaaaatctta aacaccacac tatcggtaat accgccattg
    41581 tttgcaataa aactgtttat ttttctggtg gcaatagtat tctggtctga atgtttaatg
    41641 ttttttaaag aatcgtcata cacttgttgt ttgataatat tttcatgttg cttaacagac
    41701 tcacttttga ctggtgccaa atgaatggct atgccatgaa acattttttt tgcttcctca
    41761 tcagtattac attcagtttg aagcagtatt ttaaatacgg tatgttcatt attgaggtca
    41821 gatgaaagtt taaatagatt ttttaatcgc tgcgccaata aagtgtgata gttggttagc
    41881 caaaaattaa tattcttagg atactgtgta taaataaaaa tgatggtatc agcataaaat
    41941 ccggaaggaa tacctttcca gtcagcagtg tttttaattt gtgattttgc ataaccattt
    42001 tccagaattg tgaaagtttt atattcttcc ttttgaaaag cacgaactga gtcactggta
    42061 aaaatatctt ctatttcaga actgtatttg gcaggattat cgagccactc ttgctgaaat
    42121 ttcacttgtt gcgcatagct ttgtacagct atcagcaata gtgcaaaagt attcagaagt
    42181 tttaacgaca tgttttacga aatataaaca acatcttttt ttcctttctt caaatcggca
    42241 acaatatgtt cgtgtttggt tgtagaaagt gtaagtccaa caaaatctgt tgctacagga
    42301 tagcgcttgt ggtcgcggtc aacaagtaat gccgtactta tttttttagc atcagcttcc
    42361 aaaaatggtc gcaaactatg aataagtgtc ttgccggaat ttagtacatc atcaacaata
    42421 atgacaactt tgtttttcag cagtgcagta ttgtcaattc ctttaatggt tggatgcaat
    42481 gggttttctt tgtcaataat cacttcaagc atatttactt taagaggata actctccaca
    42541 ataatatctc ttaacttggc agccataaca taaccgcttc ttacaatgcc tgctatataa
    42601 atgttttttt cactgatgtt atcctcataa atctgacaag caatacgtct aagcttttgc
    42661 tgtatttgtt catgatttaa aattacctga cggtcttcca ttttttcaat taacttaaag
    42721 cggcataaaa atagcatatt tctattactg ccttgatatt ttaagcttta ttgttctatt
    42781 tcttcgttaa tttatcccta attttaccgt aatatttaaa aatcacttca gaatgatgtt
    42841 tcagccaaag cagaaaataa taaaaagtac cgtccttgct ttcatcttaa tgttttttgc
    42901 ctttacagtt gttcatgcgc aaaagcagtt tcgttttcct gcgggcatag ctagtgatga
    42961 ttatgagaaa ggtattttga ttgtaagaat aaaatctgat tacagaaatt tatgtaacga
    43021 tagcgacata aactcctctg atttgctgaa aattttcaat aaactttcgg taactgaagt
    43081 taagaagaaa tttccaaatc acatacagcc caaggaaact aaaacatcgt ttggttttgc
    43141 gctaacagat ttgtcattga tttatacatt gaaattcaat acagatattc ctgttgaaaa
    43201 agcagcatca ttaatgcttt catgcaaaga agtacagtat gccgaaccag cttatattga
    43261 caaaccactt tacacaccca acgatacatt agcaacacca caatatcaat atcatcatgt
    43321 taacatacat acttttgatg gatgggatat tcaaaaagga gacaccaata ttgtgattgg
    43381 aattactgat acagggattg acacagtaca tgttgaactg attaatcagt taaagtacaa
    43441 ctatgccgac acgattaatg gcattgacga tgatggtgat ggttacattg acaactatcg
    43501 tggatggaat gtggcattta actcaaatga tgttcagggg tctgttccat tgcatggaaa
    43561 ttttgttgca ggcatatcaa atgcagaagt tgataacata acaggtattg ccggtgttgg
    43621 ttttaaaact aaatttcttc ctgtcagttg ttctccgggt tcaaatgtta ttgtaaacgg
    43681 atatgatgct attgtgtatg ctgccgatca tggttgttct gttattaatt gttcgtgggg
    43741 aggctttggc agctcgcaat tttcgcagga tgtaattaat tatactacat tcaataaaaa
    43801 tgctttagta gttgcagcag caggtaatgc caataacgat gcaccatttt ttcctgcgtc
    43861 ttatcaatat gtattgagtg ttggaggaac tgattctgct gatgtaaaat ggacatcatc
    43921 gcctacatcg ggaagcagtt ttggaagtta tgttgatgta acagcaccgg gtgataaaat
    43981 attttcgatt tttcctacag gagttaatcc tccatattgg tattcgggtg gaacttctga
    44041 agctgcgcca caggtttcag ccattgctgc cttagtgaaa gctcagtttc caacattaaa
    44101 tgctttgcag attggagaaa gaattcgtgc aacgtgcgat gatatttatt ctattccggg
    44161 caatgcaaat tttattaata agcttggcaa aggaagagta aacttattga gcgcacttac
    44221 acagccggca acctctgtca gagcatttga tatagaaaat actgataata atgacaatgc
    44281 tttttcgcct aatgacacta tcagaatcag tgctgtatat acaaacctcc ttgaccctgt
    44341 aagtaattta gcggttacca ttcaggcaaa tactcctgac atatcatttt tgaataattc
    44401 atttcttgca ggaaacatgg caacaatgca gtctgacagt aaccgcagca atcctttttt
    44461 ggcagtgata gatccttcaa taccaaagaa tacaaaagtt gttttcacat tgacatttac
    44521 atcgggagcc tatgttgaca ttcaacaatt tgaggtgacg gtgaatgttg attacatcaa
    44581 tgttttggtg aatgatattg gcgtaaccat tacaagcaaa ggtagaattg gatttaatga
    44641 ttcaggaaac tcgcagggaa taggttttac acaaaacgaa ggacctaacc tactctattc
    44701 aggaagttta atgataggtg ttaatgacag tgtagtgagc gatgctgtgg caggcactcc
    44761 ggcaggaact attgacgaac attttacttc gttagattat attcatccta ttgttccttc
    44821 tgtgatttcg gaatttgatt tgactactaa ttttgatgac caagcaagca gctatcctat
    44881 tggtgtaaat gtagtgcaca acacctatgt atggagcaca cctgccgatc gtaaatatgt
    44941 tattgccgag tatgtaattg ccaacaacag caataatttt tacagtgatt tatatgcagg
    45001 tatttatgct gactgggata ttaccgaaaa tacgtatgca acaaacagag caatgtttga
    45061 ttcaacttta agaatgggct atgcatttga agttacggcc aacaacaatt atacaggtat
    45121 aaaattactt actcccggtg ttgcacattt ttatgcatac aacaatgatg gtagtgatgg
    45181 aagtattaat atttatgacg gtttcagtaa gcaggaaaaa tatcagagta tgagtggtgc
    45241 tggcagggcg caagcaggca tgattggtaa tggcacagat attagtatgc tgttgagcag
    45301 cggaccattt tcaatgttgc cttatgactc tgtgaaaatt gcttttgcat tacttggtgg
    45361 cgacagcctg aatgaaattg tagctgctgc tactgctgct caattaaaat ataactctgt
    45421 tggcattacc gaagttgcaa ctcttgagaa tgagctttta gtatatccaa atcctgctaa
    45481 atcagtttta aatgttcaag ttttaaatcc aaattccggt gttgcaaaaa ttgaattgta
    45541 tgatgtctat ggacatcttg tggcagccaa aacttattta caaacaagaa ccaatacatt
    45601 aaatcttgac ataagtacac tttcgaatgg aatttacttt atgcaatata caagtaatca
    45661 gataaacaaa acaattaaaa taaatattca aaatgataaa ccctgaaaaa ttacaattcc
    45721 taaaaaataa ctttattgaa ctccttaaaa agttaaaagg tgatgaagtg cctgcatggg
    45781 gcaaaatgaa tgcacaacaa atgatagaac actttggtga tgccattgaa gatgcctgtg
    45841 gaaaaaacaa ccgcacatta ataacaccac ctgataaact tgaggccgta cgggcattta
    45901 ttcttagcga taaagacttt aaacccaaca caaaaaattc gttgatgagt gatgcaccgg
    45961 caccaactca gatgcctgat ttggcggcag caattgcccg tgtagagaat ggtattaatg
    46021 agtttgaagc tttttattca ggcaatcctg aaaagaaagt gatgaatcca ttttttggtg
    46081 agttgaatta tgcagaaaat attcaactgc tgcataaaca tgctttgcat cacctgagac
    46141 aatttaatct gatctgaaag aacttatgag tgaaggccgt aagttaattt cggtaattat
    46201 tgttgggcct gcctacccct ttcgtggtgg cattgccaac ctcaatgagg ctttgtgttc
    46261 gcatttgaat gctaatggaa taaagactga gattgtttct tttacattgc aatatcctgc
    46321 tgtacttttt cccggaaaaa cacaaatgga aacttctgca caacacttcg atttcaagat
    46381 aactcagtta atcaattcaa ttaatccgac ttcatggata aagactgttg gctatattaa
    46441 aaataaaaaa cctgacatgg tagtgtttcg ctactggctg ccttttatgg ccccctgtct
    46501 tggttttatt gcaagaaaac tacgcaaaca tggcttgcct gttgtggcaa ttgccgacaa
    46561 tgttattcca catgaaaagc gaattggtga tgtatcgctg acaaaatatt ttattcagtc
    46621 gtgtgatgct tttattgtga tgtctgataa ggttgctgat gatataaaat tgtttaaccc
    46681 taagaagcct gtaagcataa cgccacatcc tatctactct atttttggtg ataaagtaag
    46741 ccgagatgaa gcctgcgaaa agttgaatct tgataaagga tttaagtatt tgctcttttt
    46801 cggttttatc agaaaatata aaggattgga tattctcttg caggcaatgc cattgataaa
    46861 gaataaaaat cttaaactta ttgttgccgg agagttttat gaatcgaaag aagaatacct
    46921 taaactgata aaagaacatc atctggaaaa tgcaattata atgagagatg aatatattcc
    46981 gaaagatgat gtgcgttttt atttcagtgc tgctgactta attgttcagc catacaggtc
    47041 ggcaacgcaa agtggtgtta cacaaattgc atacagtttt gataaaccaa tggttgttac
    47101 taatgttggc ggattgcccg aaatggttac tgatggtgtt tcggggtttg tgactgatgt
    47161 gaatcccaac gctattgcaa atgctgttga tcgtttttat gtagagcaat tggcaatacc
    47221 atttacagca ggtgttcaat cagaaaagaa taaattcgga tgggataaaa tgattgctac
    47281 ggttaaggat atttatcaaa ctcttttaaa ataaaaatat tcgtttaaat ttgtcatact
    47341 aaattgtctg ctcaatgttt ttaagaagaa cttttttaat ctgcttttta ctatttagct
    47401 acttcgctaa tgcgcaaacc gaacgttgcg gcacacaagt ttatttagaa caatggcttg
    47461 cagaaaaccc acagttgcga aaagcttatg ataacgaaca ggcaatggct gctgcaagaa
    47521 tagaaaacag caatgcgatg acggcaacta ttactattcc tgtggttttt catgtggtat
    47581 ataatacagc ggaacaaaat gttccagacg acagaataat ggagcaactt gatgtgctga
    47641 acaatgatta caatcgcctg aatcaggatg gacagagtac accatcacca tttttgggag
    47701 tttctgctgc tgccaacatt cagttttgtc tggctaagcg cgatccaaac aataagccta
    47761 catcgggcat catcagaaaa caaacaacgg tacagtcgtt cagtacgggt gatgaagtga
    47821 aaaagactgc aacaggtggc tctaaaggat ggtcgaaaag caaatacctc aacatctgga
    47881 tttgtaacct gacaggcaac cttcttggct atgcaacaat gcccggcaca gcaaacgata
    47941 cgcttgatgg tgttgtagtg agttattcaa cgattggtgg ccctgataat gttggtgtgc
    48001 atcctactta taatttaggt cgtacgctga cgcatgaagt cgggcattgg ctgaatctga
    48061 tacatatatg gggtgatgat ggcggcacat gcacaggaac agatgatgtt gcagacacac
    48121 ccaatcaggc atcggagcat tttggcacac ccaattttcc gactatcagt tgcagcaaca
    48181 accccgatgg cgatatgttt atgaactttc ttgattatac tgacgactat gctatgaata
    48241 tttttacagc aggtcagtcg ttgcgcatgc aggatgccgt aaataacagc agggcggcat
    48301 tgctgacatc aaagggatgt atggacacaa caacggcaat tgatgttgcc ttactaaatg
    48361 ttattaatgt agaaactatt tattgccagg gcaacattac accatcggca gtgataaaaa
    48421 ataagggagc cggaactatc agctcgctta cattttctgt taagcttgat gataatacag
    48481 caacaaataa aacctggaca ggtagtttgg ttgcaggtgc aacggcaacg gttgcattag
    48541 atccgttgac agttccgaca ggaacacatt atctgaatgt gagtgtgaca cagagtggcg
    48601 atggcaatgt atcaaacaac attattgtaa aaagaattac ggtgacgggt gccactgccc
    48661 tgccctacag tcaggatttt gaggcgaaaa gatttccggc agacaattgg ctggtgttga
    48721 atgcagacaa tgatgcaact tttatgcgca gcaggctggc atcaataaat ggaagttctt
    48781 ctttgtggtt ttatgcaccc ttgcaaacgc cagcaggaag aaaagatatt gtgcagttgg
    48841 agccatttga ccttacaaca cttgcagagc cagtgcttgc ctttgatgta gcctgcctga
    48901 atgacaacag catcagtaca agtgcaagtt taaagatatt ggcatcaacg gattgtggca
    48961 gtacgtttag tgagatattt gagaagacag gaagtgcact aacaacagca gaaggcagtg
    49021 catcaaatgt atttgttccg gttgctgaac agtggcggaa agattctgtg aatctgaaag
    49081 cgttgaacag ttttaataat gtgattttac gttttgaata tactacaggc aatgccaatg
    49141 ccctttttat tgataacctg caaattgacc gccttggggt tatttatcct gatgcatcga
    49201 atgtgaatgt gagcgtgtat ggacaaagtg cttatggtgt gcttgacttt agtgtgctgc
    49261 ttttaaagac agtagatttt aaggtagata tttttgatgt tgcaggaaga aaagtatttt
    49321 caaagtcgta tagcggcagt gccgtttgcg acaggcttag cttgcatcag ttaaatgcag
    49381 gggtttattt tttcagagtt acatcgggca aggattcaaa gtcggtgaag ctgtttttca
    49441 gataggagtt tggttttgaa ttcagaatgc agagttatga attgaaaatc ggaggtggtt
    49501 tcaaactgaa acttgaggtc attgattgtt tagagtcaag aataaaaaaa ctttatagtt
    49561 aagactttca tattattttc tatatttgaa gaatattaac tttataaagt agaatgtttt
    49621 tcgataattg aaaaaaatta taaaactgaa aatcatgaaa ataaactcaa tagtttttat
    49681 tcaaattata attttattgc ttctattttc ttgtaaaaaa gataaaatcg aagcagacag
    49741 ccaaataact acgaatattg attcaactaa aaccaacatt gattcaacca ataatattcc
    49801 aacaacatac gatactattt ttcctttaga ttattttccg gcatttccgg gttcatattg
    49861 gaagtatgtg gactcaaata atgatacaac agtgcgcaag acggattctc tatatagaaa
    49921 ggattattat acttatgggt cagctgctta tgtatctgac actttttatg taccaatata
    49981 caacaatatt cctatttgga aatatgaaga acataccggc cccatttcac actcaggttc
    50041 atatccattt agagttattt tatctgagac acttccggta ggtagcgatt ggcctgtttt
    50101 ttattggtca ggaacaggtg taagcagaaa gctaattgca aaagatacaa ccattactat
    50161 ttcttcgatt gcatattacc ctactatagt aattgaggaa tactattcag ccggcccgcc
    50221 acattacatt tggatcgcaa aaaggtatta taccaagaat atagggttaa tacgagaaga
    50281 tctctataat aatttggact caactacgaa cactaaagaa ctgattgatt attttattaa
    50341 catataacag tagtgctatc agaaatgtaa atttttcagg tgatttgtta ggagtgatgg
    50401 tcagccgtgg ttgttcatcc tctaatcact caaaaaaatc tgcgctctta aatctgcatt
    50461 cctctgctca cccccgatca aaagaggcta tgctctttac aaaatgccaa aaagggtgtc
    50521 tgcccacaaa gtttactgaa taaaccacag aactaatttt aatactgtag gatcaaactt
    50581 gtcggctatg gctgattatt taattcaatc cctcacgaaa aaatttcaaa ttgcgtgtag
    50641 cctcaatttg tggtaagggg tgtatgcaag aattcagagt tatctaatta tctaattatc
    50701 aaattatcta atttgagtat tcacccctgt cagctgtggc tgataataac ttaatcactt
    50761 tctacatgct aaagcatggt gttctatgca gatactttgc ttagtacacg caccatggtt
    50821 tcgccaattt ctgcaggcga tgcaacaacg tgaatgccgc actcggccat aattttcatt
    50881 ttggctgctg cagtatcgtc agcaccacca acgatggctc cggcatgtcc cattctgcgg
    50941 ccgggagggg ctgtttgtcc ggcaatgaaa cctacaacag gttttttgct gttgtttttt
    51001 atccatcgtg cagcttctgc ctcgaggtta ccaccaattt cgccaatcat gatgatgcca
    51061 tcggtttcgg ggtcgttcat aaaaagttct actgcttctt ttgtggtggt accgataatg
    51121 gggtcgccac caatgccaat ggcagtagaa atgcctgcac cagcttttac tacctggtcg
    51181 gcagcttcgt aggttagtgt tcctgattta gagacaatgc ctatgcgtcc tttctgaaat
    51241 acaaagccgg gcataatacc caccttacac tcaccggcag taattacacc ggggcagttg
    51301 gggccaacaa gtcgcgcttc tttgtcgcgc agatagtcgc gcacctgaat catatcttta
    51361 gtagggatgc cttctgtgat ggcaacaatg agttttatgc cagcctgtgc agcttccata
    51421 atggcatcgg cagcaaaagc cggaggaaca aaaatgatgc ttacatcggc accggtttgt
    51481 tttacagcat cggcaacggt attgaaaaca ggtcggtcta aatgtgttgt tccgcctttg
    51541 ccgggggtta caccaccaac aaccttggta ccatactcta tcatttgtcc tgcatgaaaa
    51601 gtgccttctg ttccggtgaa tccctgaaca ataatttttg aatctttatt gactaaaacg
    51661 ctcattgctt attgaaaatt aaaaagttgc gcaaaagtaa attttatttt tgaataatgt
    51721 gactgacggt ataaagcagg gtgcttattg atttaataat taggcttaaa attttgattg
    51781 tttacagcgt aggatttaat aatttttggt gatggtgaat cgtaaaaaaa atcatctcac
    51841 aatttagaat gcaatggtga gatgatttat atataatgag cctatggctc cataaatttt
    51901 tccattgcat tgaggctgac accggtgaag gagaagccac catcgtgaaa gagattttgc
    51961 atggttacct tgcgtgtgaa atcggaaaac atcattacag tgtagtttgc acattcatcg
    52021 gcagaggcat tgccaagagg cgacattaca tcggcatagt tatagaaggc gttgaaacct
    52081 tttatgccac tgcctgcagt tgttgcagtg ggcgattgag aaatggtgtt tactcttact
    52141 ttttttgcca tgccataatg atagccaaag ctgcgtgtga tgctttctaa aagcgatttt
    52201 gcatcgccca tatcattgta atgagggaag acacgttgtg ctgcaatgta ggtgagtgcc
    52261 actacactac tccactcatt gagggcatcg agtttcatgg ctgtctgcaa tgttttgtgc
    52321 aggcttagtg cagaaacatc caatgtttta tgaaaatatt cgtagtctaa tgctgtgtaa
    52381 tgtttgcctt tacggacatt gggcgacatg ccaatgctgt gtaaaacgaa atctaattta
    52441 ccgcctaaaa tttccattga tttggtaaac aggttttcga ggtcttcaat agaagtggca
    52501 tctgccggaa tgacttgcgc attacattct gctgcaagtt tgtttatttc gcccatgcgc
    52561 atagcaacag gagcgttggt taatgtaaac attgctcctt cctgatgtgc acgttgtgcg
    52621 gttttccatg caatagagtt ttcgtctaat gcgccaaaaa tgattccacg ttttcctttt
    52681 aacaaattgt acataagtta tgttgttttg cggctaaagt aatagaaatt gatgaaactg
    52741 cattaatctg tgccaccggc aatgcgttta ttcagtggcg aagcactatt gtcaatggca
    52801 ggcaactgtc cgctaaagtg cacaacggca gaagtatcgg tgagcgattg cagaaacaga
    52861 atcagctttt tcttttcgtc tttagtgagt tttaatttat catcgggaag ggtttggtcc
    52921 ggaaggtgaa tgcccagccc tgccccacct cctttgtcat aaaaatcaac aacctcctga
    52981 agcgttttgt aaactccgtt gtgcatgtag ggtgcagtca ctgcaatgtt acggacagta
    53041 gttgttttga atgcatattt aagctcgtca tttttaaaaa cattgtagcg cccaaggtcg
    53101 ccatctaatt tataaggacg tttgttggtt gagggcacac ctataatatc aaactcactt
    53161 tctctgtact gtggcggtgt ggttccgtta aagagtcctg caaaatggca gtgcccgcat
    53221 ttggctttgc ccataaaaat attgtagcca tcaatgacag gttgttctat tgtgcctgca
    53281 ccacgcatgg ctttatcaaa aggcgaatcg aacgacagtt gtgtgcgctg aaattctgcc
    53341 aatgctttta gaatgccttc ttcgcttatg gcagtatctt ttgttcctgc aaaagcctga
    53401 ctgaatattt ttttgtaggt tgaagactga ttgagtttgg caacgacaac tttaaaatcg
    53461 ccatgcatct ctctctcatt ctgaacaaca gtttttgcct gatcttcgag aaccaataca
    53521 ctggcatcca taaacagatt gcgctgcaat gcagcattta aaattgttgg tgtgttgcga
    53581 ggcagtgtgt cgttttttaa aaaaccataa gacttaggaa ggccatcggt ataagccata
    53641 ttgggtttat ggcacgatgc acatgacaca gcattgttga cagaaagtat agggtcgaaa
    53701 aataaaagtt tgccaagata aggaagcata tctccgttgt tgctgcgctg aagtttggta
    53761 aagaaatagg tgttgtaggc ttctttctca aaaagtgttg tagcatcaag atttaaggct
    53821 cttatatttt gcgggtcgaa ttttaaaata ttttttcttg cattgatgag tgataaattt
    53881 acaggcacaa catagttgcg atagaatgta aagtaatcga actccgcacc ttggtttact
    53941 cttatttgct gacggtcgag aaaagctacg gcagagtcaa tgctgttgtt gaccattgaa
    54001 aaatcggcat tgcgataaga gatatttaaa atattgctga tagaaaacag tgctgcttta
    54061 gtttctggta ttcctgtctt aataatctga cagtcgaaat tgtttatacc caatgtgtaa
    54121 atttttacaa ggcgcagttg cattatttcg aacaactgat aatcgtaggg gtgcagtgtt
    54181 ttaaacgatg tggctaactt ggcagccaga ccaagtgtgt gattgatttc atcgagcagt
    54241 tgtgctttat tcaatggttt ttcggaaatt atttcttcta ttacctgcaa gccatgtggt
    54301 ggacgaacat atttgctctg cggttcttct tcgaccattg gaatgggcgg gccgttgagg
    54361 tcgcgttctg tataagcaaa ataatatccg gtgaagcctt cgagtttttt ataggcaagg
    54421 cgacaggctt taaattgtgc aatcaactgc tgtgagttta ctttattgct gccggcaaga
    54481 attttaagtt tttcgagttc tccagagaaa tctaatgacg attttgagag ataggcttct
    54541 aatgcctgct gctgatattc attttctttg accgttatga acgaccattg cagtaatgct
    54601 acaatgatga gaattgctga tgttgttatg tatatgcttt tcttaaatgg catctttcaa
    54661 aaatgacaaa aaactatata tgtgctttgt tgagagaaat aaaattatta acgcaatagt
    54721 ggttatattt tatgattgta aaaatgattt ttgtcaatta aaactttcta tgaaattaaa
    54781 ttcaagcaag agaaaaattg ccgtatataa aaacaatgct gattgccatt aaatagcatg
    54841 ataagtagta ctttcgcgct tcaattaaaa agcagtgaat tatttatcgg tagaaaatat
    54901 aagcaagagt tttggcgaca gagtattatt caacaacatt tcgttcggca ttgccaaagg
    54961 acagaaactg gcattggtgg caagaaatgg caccggtaaa acatcactac tgagaatttt
    55021 agcaggactt gatgttcctg atgaaggtac tgttactttc agaaatcagt tgaaggtgag
    55081 ttatcttact caggaaaata ctttaccaca gttggcaacg gttgcagatt gcctgacaga
    55141 gaacacacac atttctgatg ccttgctgaa tgaaaaacca cattatgaac tgactgaaga
    55201 agagcaaaca catttgggaa aaataaaatc tgttgcagga aaattgggtg ttccattttt
    55261 tcatcaaaag gtgaatgaac tttcgggcgg gcagcaaaaa cgtttggcgc tatcgaaagt
    55321 gctgattgat gaggctgact ttatcattat ggatgagcct accaaccact tagacttaga
    55381 catgattgaa tggcttgaat cgtttttagc atcaacaaac ataacgatgt tgcttgttac
    55441 acacgaccgt tattttttag atgaaatatg tgatgaaata cttgaacttg aagacgggaa
    55501 actgtacaga cataagggca actacagcta ctatttagag aagaaagcag agcgtgatga
    55561 aatggaatcg gcaaacctgc agcgttcaaa aaatctattt cgtacagaat tggattggat
    55621 gcgaaaacaa ccacgggcac gtggcacaaa atcaaaatcg cggatagatg cctttcatgt
    55681 gcttgaagaa aaaactaaaa agaaaaatga acaaggtaat gtggcacttg acatcaacat
    55741 ttcgagaata ggaagtaaaa ttctggaaat acaccacatc agcaaatcgt atggcgacct
    55801 gaaactgatt gacaactttg actactcgct gcgtaaaggc gagaaaattg gaattgttgg
    55861 cagcaatggt gcaggaaaaa gtacactgct gaatatcata atgggaaaag agccttgcga
    55921 taaaggtaag attgtgatgg gtgaaactat tgtgcccgga tattttgagc agcaatcgaa
    55981 aaatatttca gatcagaagc gggttattga agtgattcgt gaaattgctg actttattcc
    56041 gatgagtggt ggacgaaaaa tatctgcatc gcagttgctt gaacgttttc tatttgaccc
    56101 gtccatgcat tttcagtatg tagaaaaatt gagtggtggc gaaaaacgca gattgtattt
    56161 gctcacggtg ctgatgaaaa acccgaacct tttgatattg gatgagccaa ccaatgattt
    56221 agatatacag acactttctg ttcttgaaga ttatttggca tcgtttgaag gctgtgtgat
    56281 tattgtaacg cacgatcgtt tttttatgga cagggttgca gaccacatct gggcatttga
    56341 aggagatgga aaaatcagtg ttctgaatgg caattactat gactacagac aaatgctgaa
    56401 ggagcaaaaa agagagcaga aaaaggaagc agcagcagta gtgagaacaa aaccggaaga
    56461 aacaacagcg aataaaaaac gcaagctttc attcaaagaa aagcaggagt acgaaaagtt
    56521 ggagaatgac attatgctgc ttgaagagga aaaaacaatg ttggaacaac agatgggcga
    56581 agcagaactt acacatgaaa agttaatgga agtaagtaat cgctttcagc aggtgctgca
    56641 tgacattgat gcaaagacaa tgcgttggat ggaacttgca gaaataggct gatatttact
    56701 tatggtgttt tagtcaatat aaaaccatgc tgatagcctt tgtaatgatt gcagataagg
    56761 tagtttttaa ttttactttc tgacttacgt aagtaaaaat gttcaggcaa aataccggag
    56821 tttgttgcag cctgaccaat aagccaaagt gccaatgccg acaccgttgg aaattcacca
    56881 aaaaaatgtt tatatctgat gattgcagat tgctgaaaat gactttcaac agcataatgc
    56941 aatgtttcat tcctgacatc gccattgtga cctgaaacca acatagtttc cgaaagattt
    57001 aaagcatgtt tatcgcaaaa ttgttttagc tgttgtaaaa cttcgtcaac atcagtactg
    57061 tgaagtgtgc ttacgtcaac aatttctaac agtgcacttt ctgaattgtt acttactgta
    57121 aacgctgcac aaccctcacc ggcaatgctt ccttctgttt ttgattcata caaaccattt
    57181 acaggaattt cgttcttata ccatccaccc aagcgttcta tattgtaatt atagtcagaa
    57241 atttcatcaa tgccaccgag caataaggtt gagtttttat tttctactaa atacatgaaa
    57301 gcatcaagca aagcattttc aaaagcattg ccacggtgaa catgcgttat gttgtagcca
    57361 cgacattgat gattcattcc aatggttgaa gcaatggcat tgggtgtgct ctgcacaaaa
    57421 ttggttggtg ttaatgtgcc ttcatcatac tgcacaatct gattaagaaa acgaatacaa
    57481 tcttccatac cgccatttgc agtacctatt attattccgt ttacagggtg attttccaga
    57541 ttgagtttgc tcacagcacc gcaggtcatg cgcacagctt tacccattct tcgcaaaacg
    57601 gcaggtgcaa tcatactgta aacaggttcg cgcgccataa gcttttctgt ttgcaagcta
    57661 tgttctgaaa gatctgtttc gccaaaagta tttctggcgc tgatgcatac tgaagattta
    57721 atgtaaatac ccatgatttt gaaaaagcaa gattaagaaa tttggcttca tcgaaaagta
    57781 cttctgcaag caaattattt aattaacaat tttgttttta taagcttcct taatctttca
    57841 taatctgcaa ctgcctatat gcaacgaaac tgtatttttg gcagtcttta tttcagcatt
    57901 gctgctttaa attattatgt attcactttt caagaaagag ataaacagtt ttcttaattc
    57961 actaatagga tatatttcca ttgctgtttt tttaattgtg attggattat ttctgtgggt
    58021 gtttccgggc aacgacctca acattgcaga cagtggttat gctgccgtag atagtttatt
    58081 caacattaca ccatgggttt acatgttttt gattcctgcc ataaccatgc gcatgttttc
    58141 ggaagaaaaa cgtacaggga ctttggagat attgcttacc aaacctttaa cagagtttca
    58201 gattgtattg gcaaaatatt tatcaggatt ggtattggta atattttcat tgctgccaac
    58261 attggtttat gtggttacag tttataaata tgctgcaccc gtaggaaata ttgatttggg
    58321 tggaattgca ggaagttata taggattgtt ttttctggca tcgggatttg ttgccattgg
    58381 tgtttttgct tcatcattag cagataatca attggtagca tttatcatag ccatgttttt
    58441 atgcctgcta tgctacacag gatttcagtc tgttgcaaca attctggcaa caggtgttgt
    58501 tgcaaacctg atagatcaac ttggtattgt ggcacattac aactcaatga gcagaggtgt
    58561 tattgactcg cgcgatgtaa tttattttat cagtttgaca gcgttatttt tagtgtttac
    58621 ccgattaaaa ctcgaaagca ggaagtggtg atatgaagaa gaagatacgc agtgccaacc
    58681 tctatcagtt tttgcttttg cttttagtga ttgcaggcct caatgctgcc ggtaaatttt
    58741 tattctggcg ttttgacctt actactgaaa aacgttttac cctttcgccc acaacaaaag
    58801 aaaaactaaa gcaacttaaa gatatagtat atgtaaaagt ttatctgaca ggtgatttac
    58861 ctgcaggttt tcagaggcta agttctgcaa cacgcgacat gctgatggaa atgaagagct
    58921 atgcaggctc aaatcttgag tttgatttta ttgacccatc ggcattagca gacagcaagc
    58981 aacgtaatga gctttttaat caacttgcag acaaagggct tcaaccaacc aatatctctg
    59041 agcgaaatgc agaaggcaca tcagaaagaa ttttgtttcc gggagcaata cttaactacc
    59101 tttctaaaga gcaaccacta ctactgctga aagatagaat gggtgcatct gcagaagaga
    59161 tggtgaacac ttctattcag aatctggaat atgaaattat taatgccatt tctaaaattt
    59221 cgacaagaaa gccagcgaca attggtattt tacatggtca gggagaattg gacaaacctt
    59281 atctggctga tttttataaa tctgtatctg cctcctaccc tacgcattat gtgcaaataa
    59341 aaaatcagct caatgcttta caagattatg attgtttggt aatcgcaaag cctgactctg
    59401 catttgatga aaaagataag tttataatag atcagtttat catgcgtggc ggcaaggttc
    59461 tttggttgct cgatatgatg agtgctgata tggatagttt agcaagaaaa aacgaattca
    59521 ttgctattcc taaagaactg aatcttgatg acatgttgtt tcgttatggt gtaagactga
    59581 ataaagactt ggtaatggat ttacagtcgg taccgattcc tatactgaca ggatatgtag
    59641 gtaacagacc acagaattct ttgttgccct ggttttatta tcctttggtg actccaacat
    59701 caaaacatcc tattgtaaat aatctaaatg ccatacgctt tgcatttgcc ggaagcattg
    59761 acaccattgt ttctgcgcac attaaaaaaa ctatattgtt acaaagttcg cactactgca
    59821 agacagttca atcaccggca gtggtagatt taaatatcct gcgcaaagat cctgatgaaa
    59881 aagaatatcg taacccacct gtaaatcttg ccgttttgct tgaaggttca ttccgctcta
    59941 attttgcaaa cagaattcca tatcagattg caacagacag caccatagga tttaaagcag
    60001 atggtaaacc aacgagcatg attgtgattt ctgatggtga tgttatccgt aatgattata
    60061 aacgtagcag caatacggtt tatccgcttg ggatggatcg ttataccgga cagttttacg
    60121 gaaataaaaa tctattgcta aattgtattg attatttatg cgataattcc ggattgatgg
    60181 ctcttagagc aaaagaattt aaactgcgcc tgttagataa aacacgattt gaccctgata
    60241 aaaaaatgct gcaaatagta aatgttgcat tgcctgttct tttaataatt cttttcgcaa
    60301 tgattaagtt gttgatgcgg aaaaagaaat tctcctgata gaataatgaa taaaaaccga
    60361 attttacttc tcatagttgt gttgttgctt tctgtaaccg gatattatta tttcaacaac
    60421 agcgatagta caattaaaaa agaactgcgc gattttgcag ttgccgatac ttcttcaatt
    60481 gataaaatgt ttctggctga taaaaaagga aacaaagctt tgctggaaag aaaaggccct
    60541 gccctctgga ctattaacgg taaatatcct gcacgtcagg atgcaattaa ttttttgctc
    60601 aaaacaataa aacaaattga agtacgaagt cctgttggta aagctgccct gcaaaacgta
    60661 ctgaaagagt tgagcagcac aggaacaaaa attgagattt acagcaaagg cgataaactg
    60721 aaaacttatt acgttggcgg accaacacag gatatgcttg gcacctttat gtatcttgaa
    60781 aattctaccg taccctttgt gattcatatt ccgggcttta acggatacct tacacctcgt
    60841 tattttgcca gtgccgatga atggcgcagc cgcattattt ttcaatatgg cgaagggcaa
    60901 attgcttctg ttatggttca gaataaccaa catcccgatg agtctttcag aataaaaaaa
    60961 gatgatgatc attatgttta ttattcaagc ctgactgcaa ctgcaccggc agaaattttt
    61021 caatctcagt tagttgcata cttagcaaaa taccagctta taggatttga gcggccaacc
    61081 tacgaccttg atgttgccta tcgcgattcg ttattaaaga cagggcctgt cagaatactt
    61141 gaagttgaag gtgttgatgg caaaaaagat aaaattgaaa tgtatctgaa gcctgttgac
    61201 acgggaacca tgaccagtgt tgatgaaaga acaggccttt tgcgcgaatt tgaccctgac
    61261 agaatgtatg cctcttggaa taacgacacc aatttgattg tggttcagta tcatgtattt
    61321 gacaaactat ttagtaaacc cgacagaatc aaggggttcg gtagtcttta atttttcttt
    61381 aatttagcct tcacaaagtt ccatctgatt atatttgcca cctaactttt gatatttatg
    61441 aaactaattg tttccagcct tgtacttcaa aaacatttac aatctatcag cggggtttta
    61501 accactaact ctcccctgcc gattttagac aattttctgt ttgccgttaa tggcagtgag
    61561 cttactttat cagcttctga ccttgaaaca acaataacca caagacttaa agttgaatct
    61621 aaagacaaag gcattattgc catcccgggt aaaattctgc tcgacatctt aaaaactttt
    61681 aatgagcagc cgcttacttt taccatcaat gaaaaaaacc ataccattga gataagctca
    61741 gattatggaa agtataaact caatggtcag aatggtgaag actttccgcg cataccggtt
    61801 ctcgaaaaaa gtaaccacat tgaattgcca tcatctgttt tgcatgaggc gataaataaa
    61861 accatttttg ctacgggcaa tgatgacttg cgccctgtaa tgtcaggtgt attttgtcag
    61921 ctttcgcagg acagcaccat ttttgttgct actgatgcac atcgtttggt gcgctatcgc
    61981 agaacagata taaagtcaaa agaagcaggc tcatttattc tacctaaaaa gccgttgaac
    62041 cttctgaaaa atattttacc tcaggaagaa acacgcgtaa aggttgacta taacgaagcc
    62101 aatgcatttt tcagttttga caactatgat ttgatttgcc gtttgattga tggacgttat
    62161 ccgaactacg aagcagtaat tcctgttgac aatccaaatg ttttaactgt tgacagagtt
    62221 gcatttctga actcgataaa acgtgtttct attttttcaa acaagacaac ttatcaggta
    62281 aaacttgcaa taaagggaag cgaattggca atttctgctg aagatgtaga ttatgccaac
    62341 gaagccaatg agcgtttaac ctgctcttat gatggatctg atatggacat tgcatttaat
    62401 gcgcgttttc ttgcagatat tgtttcaaat cttgacaccg aagagattca tctgcacatg
    62461 tcattgtcga accgtgccgg attattaact cccggaaaag accataccaa tgcagcagaa
    62521 gatattctga tgctcattat gcctgttatg cttggctgat aaattttttt gccggtttat
    62581 attgttaaga atgtaaattt ttatgtgtta caatattgca ataatggaaa cccggcagca
    62641 aaaatatgcc gaacgttata aggatgttat tggagcatca ttcagctttg aaacagcaca
    62701 acctgaatta ccactttact attttgtcag tggatttgca catccgtttt taccagtaat
    62761 caaacatgat ggactatttt tatttgaatg gggactcata ccctcatgga taaaagatgc
    62821 taaaggcgct tcggaaataa gaactaaaac actcaatgcc gttggtgaaa cagttttcga
    62881 aaaaccatca ttcaggaaaa gtattgttag caatcgttgt ttgcttggtg tttcaggctt
    62941 ttttgaatgg cgcgatgtaa acaaaaagaa atatccttat tttattaaaa caaacaactc
    63001 tgaattattt tctcttggct gcatttatga aacctgggtt gataaaaaca ccggtgaatt
    63061 acataatact ttcagcatca tcactactgt ggcaaatcca ctgatggaaa aaatacataa
    63121 cgtaaaaaaa aggatgccgt tgataattgc agaagaagat gaaaggaagt gggtggatcc
    63181 aagcacaggt attgacgaaa ttagatcact gattaaacca tataatgaat ctgaaatgac
    63241 ggcctatacc atttcgcagg cagcaaacag tgcaagggct aatcgtaatg ttccggagat
    63301 tttaaatgca gtaaattacc cggaattggc actcttggat acaatctgaa attattgacc
    63361 aaataaattt ctgacttctg caatttttct tcttgaaact tcgatggcag aattatcact
    63421 cagcaccaat ttgttttcgt tttcaaatga ccttatatat tttttgttga ctaaatgcgc
    63481 tctgtgtact ctgatgaaat tataactgtt aagtaaatcg tcaaactctt ttaatgtttt
    63541 tgatgataga acaacctctt tattgccaca agtaatctta gtataatttc catcagcttc
    63601 gcatctgata atttcatcgg gaaggagaaa atatgtttta tttgaagtgt taacggccaa
    63661 tcggaacagc ttatcttctt ctgacttaat attcctgata aaattattta tgacagattt
    63721 tctgtcgtac tcgcttttct gtctgtcgag aaatttctgc actgcacttt taaattcttc
    63781 tttattcaga ggttttagca aataatctaa tgccgaaaat ttaattgctt taattgcata
    63841 ttggtcaaaa gctgtaacaa aaattatttc aaagtcatgt tcctgtattt tctcaagcaa
    63901 atcaaaacca ttactgtctg gcatttcaac atctaaaaaa atcaaatcag gtttgtgaaa
    63961 ataaatctgc tttagtgctt ctgctgcatt agctacttca gcaactaatg taatttgagg
    64021 gaagtatttt gttatcaata attttatctg ctctctaccg aacatttcat catcaaccac
    64081 aattgccttt atatttttca cctcactaat gtaattattg tacaaatata tttttagccg
    64141 gcagaattat ttactttcca tagctttaaa gaatcaacca ttgtcataca ttgccttctt
    64201 ataagtaata caacaggcat taacttgttt ttctttatag tttctctctg cttttcgagt
    64261 tctgaaattt tttgcacaca gtcattcaaa ccaacagaag caatgagagg tttaatgctg
    64321 tgtaataaga caattacatg tttctcttca tctcctttta aagaaatttc aatctcatta
    64381 aaaacctcag ggattctttg gagatacata tcaacataac gcaacatctc ctgaatatcg
    64441 ccatcagtaa agtctctcag atatgctaaa tcaactgaag atgcaattgg cgcaacctta
    64501 attctgctgt caattttctg ttcatcaaca tcacctttaa accggtttat tacatccgtc
    64561 agttcagaaa ttttaaaagg ttttgaaaga tagtcgttca tacctgcggc aatacatttc
    64621 tctgcttcat gttgcgatac atttgcagtg agtgcaacta tcggaatttc tccaactgat
    64681 tctttaagct cattccgaat aatctgagta atttcatacc catccatttc aggcattgaa
    64741 atgtccatta aaatcaggtt gaacttttgg tttttcagta tttctattgc atcccttcca
    64801 tttccaacaa tttgcaattt tatatcacgc attttctttg aaagtgcatc tttaactagt
    64861 atctgattaa actcattatc ttcaatcagc aaaacagaaa acgcatcttt atcaattatc
    64921 aactgaatgg gaacttgctc agatttattc gtctctgcta aagcatctgc aacttctact
    64981 tctattgtaa aggagaatat tgtgccctga ttttctacac tactaacctc tatattccca
    65041 cccattaact gaacaagact ttgcgaaatc gtcaaaccaa gacctgtacc accatatttt
    65101 ctagaatttc ctgcatatgt ttgctgaaaa gattcaaata ttttttcttg ctgaccagca
    65161 ggtattccaa taccactatc cgatacttca aaaagaagat cacaataatt tagtccatgc
    65221 ttttgtgatg ctcttacttg attaattgaa acaataacaa atccacgttc agtaaattta
    65281 attgcatttc caactaaatt tataagtact tgtgtgatac gaaagggatc tgctatgatt
    65341 aattcaggca cattttctcc tacagaaaat ttcaaatcga gttctttact cgcagcttta
    65401 tgagaaaaag tctgataaac atgattaata gtttctctga atctgaatgg agttttttca
    65461 atctccattt taccggcttc tatttttgat aaatcaagaa tatcattaat aatgaccaac
    65521 aaactttctg acgattgttt aatgacttgt aaatacttac tactttgctc atcttgctta
    65581 gtttcctgca acaattcagt cattccgaaa atggcattta atggcgttct gatttcatga
    65641 ctcatattag ccagaaattg ttgcttaaat ttttcactgc gttcagcacg ctctttctcc
    65701 tgtgcaatga tgatgttttt ctgctctagc tgctgtgctg cccttttctt taatgaaaaa
    65761 cggttataca gatttaacaa gatgagaaat agcatcactg ctcctacaat aaaaatattc
    65821 tttagttgtc gttgccattc ggcattcgat ttctcaatag ccttatctct attgagtatt
    65881 tcaatcattt tatctttctg ctcagtttcg aattttgttt ttatctcatc aagtcgggct
    65941 tgctgctttg tttcattgat agtgtcttta atttccgctg cttgcttaaa agtcagatat
    66001 gcattctgat aatcatgttt cattgcatac aactcactta acgaatttag tgtctgtaat
    66061 tgtaaagttt gaaatctatt gctatctgct atctgcaaag ccattttcat ataaatgatt
    66121 gcagaattta ttttaccata tttcctgtaa gcatctccta tcaaggtaag ttcataacat
    66181 aaatcattct ggctgcctga cttttcaaaa ttctctctgg cgaactcaaa ttgcttcaat
    66241 gccatatcaa aatttccttg tttgagataa atacttccaa tactctctgc tgcataggcc
    66301 gttccactta tgttagataa cttttgatac atagcagaag attgattcag gtaatgaaat
    66361 gcactatcat acatttctaa accaagatat gcattaccaa ttccatttat gcaatcagcc
    66421 agtttattac tatcatttat ctgctcattt agtgctgcag cttctctaaa ataaggaatg
    66481 gccttattat attctccttg gctgttatac acaatgccta tagaatataa tcctgccggc
    66541 tttcgaatag catcagtaca tgcattggat aaatcaacac tcttcagata atactctaag
    66601 gcatcagcat ataaactctg attaaagtat gtgctgccga tattggataa tactactgcc
    66661 acattacatt tatcattggt ctgttcaaaa tattgaagag ctaatgtaag atgcttcttt
    66721 gctttagtat aatctccctt acgagaataa caccaaccta agctattata actatcacca
    66781 atgcccttta aaaacttgat tctttgtgca atctcaagtc cctttttacc atagtatatt
    66841 ccactgtccg tatttgcaat actgtaatta aaacataaat cacgataggc ttccactctt
    66901 gcagaatcag tagtagccgt ttgaagatta tttaaaaaaa caggaattct agatgcatcc
    66961 tgcgcataaa cagaaattaa cggataaaaa gtcagtaaaa taaaaataac aaaacgcatc
    67021 atctataatt agttgccaaa tgagttacca aagtaagaaa acttttaata gaatacttac
    67081 cgtttaataa ttaaacacaa ccattcaata agttaatgca atatgtatat catatagcaa
    67141 ctaatattgg attataaatt ctttcaaaaa atatgtttaa ctattaaaaa caaaaaatat
    67201 gaaactcctt tacaccttag cactttcact ttttaccgca catttaacat ttgctcaatt
    67261 cagtagcgat ccctcacaac caatgccggt ctgcaatgct gcacatggac aatctaacgt
    67321 gattagtctt cctgatggta atgatggaaa ttatcttttc tggctcgaca acagaggatc
    67381 tgttaataca acaatttatg cgctctatgg tcagcattta gattccggtg gaaatccttt
    67441 atgggcaaac aatggtaaag tgattcttga taaagatact gtttcaataa aatcattcaa
    67501 ttgtgtattg tttgatggtg gtattttagt tacttgggta caaggtcaga attcatataa
    67561 tgattcaatg tttgtaaaga aaatcaaccc ttcaggcaat gatgtttggg cacagccaac
    67621 tttaattgaa tcgacaaatt tttcggcttc aattttagga attgaaactt atgggataaa
    67681 ggcaatcccc aatgactccg gtgcatttat taactatagt gtagtttata taggtggatc
    67741 aagcggaatt aaatttaatc ggattaattt caacggagta aaacaatggg ctgcctcaaa
    67801 aacaaattcc ttgcccggct atactttctt tacttatccg ggaatacaaa acactttcta
    67861 ttcattatcc agaggaaatg gaatgggatc aggaatttat attcaaaaat ataatatgca
    67921 aagtgtacca caatgggcaa atccggtaga tatttctcaa ggtgtttcag gaaatggact
    67981 tgcagggaat tgcaaattcc tgacagataa ctcaggaaat tcttatgtag tttgggatag
    68041 ttattcatct gccgatatcc ttctgactaa gattgatgct gccggtaatt ttgcatggac
    68101 tccatcatat aaatcagttg ttaacttggc aaatacttta caaggcagat cagatgctgt
    68161 tttaaataac aacaacttgt ttatcacttg gagcgataac agaattactg gtcaggctac
    68221 agcttttaca caaaagtata atgcaagcgg tgctgcacag tggatagcca atggagtaca
    68281 attaggggta atcaactcca attattgtta tccaaaaata gcatatagtg acagtggctc
    68341 tgtagccata gcatatatta tgggagcacc ttgggaatta tatgtacaac gtgttcgaag
    68401 caatggtact ttaacttgga gcggcagtgg taaaatactt gcaagtgacc atagtttcat
    68461 caactatgca gactatacca tcacttctga ttcaaatgga tgtaatgctg ttttttggca
    68521 tgaatcaagt acaggagatt tatatggtgc aaaagtttgc agtgatggta cgcttgtaac
    68581 tgttgcagaa ctattaaata aagaagttaa aatatttccg aatccgacag cagatcaaat
    68641 taatattgag ttgggctcat cgaacctgat taatgaaatt gaaattcttg atatgcaggg
    68701 gagaatatta aaaactgtga ttattgaaaa cggacagcag taccaaacgc ttaatgtaaa
    68761 tgaattttca aaaggtgttt atctgataaa gataaaagca ggtaatgaaa gtatcatcaa
    68821 aaaagttatt attaaatagc ctttaaccaa aacagcaaaa ctccggcata aagccggagt
    68881 tttaaatata ataacgtcca tgtcaaaaca acctccaaaa attaatcatg aaataattcg
    68941 tgaaagggca ttagatcgaa ttttatttat tcttgaaatt gctttggtta ttaccttaat
    69001 agccttatgt ggtgtaatta ttacttcact ttgagaaatt aatgtataag ttaaattcta
    69061 caagaaatca ttacgagatt ttaagagaaa cttatgttaa attacgattt aaggtttaag
    69121 ctacatgtgc caaacataaa tgttataaaa gtatttatat acttcttttg gattcctaag
    69181 aaatagttta tacgtttgca ctacctaatg tagtttaacg gatacatggt agcatgtttt
    69241 atattttatg cacctgtaac ccataaaaag gttatattac atcaacggga aattgttttt
    69301 ggaggcaacc aaaattccgc tggcttgatg gcaatgcttt gccccatttt aagaggtaaa
    69361 aaatgtcata gattcttcaa aaatctacaa cttttctcta ctcaatttat tcattcagga
    69421 attcggatta acaaacctat acaagttaat cttaatcatc atatacatca cattcacttt
    69481 aattatttaa actaaaagtt atgcaacaac attccattat gttatttttt atgctgcttg
    69541 ttggtattgg cactatattt tttgtgtcaa tctttatgtc ttcactcaaa gctgacaatg
    69601 attcaacagg tgtaaagaac ctgttaaaaa aacgtttttg gtttacctta attctcattg
    69661 ttgtacttgg catttttgct gcagcaactt taccaaaatc cccatacttc acttatgcga
    69721 atgaaacacc ttcaactgtg attcatgttg gcacaatgca attttcattt tttctttcca
    69781 ctaaacccat tgaccctaaa agtccaagtg gagaaacaat tgaaatcccg cacgaccaac
    69841 tcgtagaatt cagagtaaca agttttgatg tcaatcatgg atttgctgtt tacgatcctt
    69901 caatgcgatt gattactcag gtacaggcaa tgcccggcta tgtcaacaac ctacgctata
    69961 aatttactga acctggcgag tatcacattt tttgccttga gttttgtggc acagggcatc
    70021 ccgttatgca aacaacattt aaagttattt aataaaaaaa ttaaatcatg aatatagatt
    70081 cacttaagct taaaaaaata agcgcacttt ggataattgt cattcttgta ctttttattg
    70141 caagtattgt acttggaatt cttatgaggc tcaatcaggg aaaagttatt gaacaatcgc
    70201 cacttacttt ctatacaaac atgacaacac atggtttaac catgattggg atttggtttg
    70261 ctgcaggaat ggttgccgta aactatcttt tacaacggta tgtgaagaca agttatggtg
    70321 ttaacttgtt tgcctttatt cttactgtta ttggtgttgt aatgctttgg gtaactacct
    70381 atgcaggtaa gtttagcaca ggctggactt ttctctatcc attaccattc agagatctcg
    70441 gttcggagag ttggtcaaca ccgttgtttc ttatttcgtt aacaatatta ggtgtaggat
    70501 ggctggtgtg gagtgttagt ctgatggctc agatttttaa aaaatataca ttggcccaag
    70561 cctttgcatg gcaacatttc aaaagtaatc caactgtaga gacaccacct tttatattga
    70621 taacaacagt gacattagtt ggtgtcattg cctcgttaat tgcagcagta attgttattg
    70681 tattgtattt cgcagaatat ttctctcatg gagattttgt caatgatgct ttgctgatga
    70741 aaaacctgat ctatttcttt ggtcatacca ttgcaaacga aatgctttat ttaggcttgg
    70801 ctgttatcta tgaaattttt ggagaagtaa gcgacaggcc aaaatggaaa atgacctggt
    70861 atatttcatt ggcatggaat tgtgcaatgg tatttatttt aactgcattt tttcatcacc
    70921 tatacatgga ctttgtgcaa ccacaggctt tgcaaattgt tggacaattg gcctcatatc
    70981 ttgcaagtat tccatctgct gctgtgacgg ttttcagtat tgtagcagca gtttatggag
    71041 ttaaggtaag atggtctatt gtaaacctat tgttctttct tggtgttgcc ggatgggtaa
    71101 ttggtggctt aggggctgtc attgatgcaa ctatttcaaa taatattatg ctgcataata
    71161 cgctttgggt tcctgcacac tttcatacct acaatgccat gggaaacgtt ttgtttagca
    71221 tcggtttctt ttactggttc tctacgcaat atgttgacaa attagtttta agtaagctta
    71281 caaaatacac tttaacactt ctaattgtcg gtggcattgg cttcttaatg gcattctatg
    71341 tggccggtgc aaattcaatt ccaagaagat atagtacata tcctgttgaa ctcacatcag
    71401 ggccttatct tgctaaggta ggtgctttgt ttgcaacaat atatcttatc gctattttga
    71461 tattctttta taatattgct aaacgatgtt tcgttatttt gtctgcttct tcgcgctaac
    71521 atgttttagc tttttaggtt atgctaatga taatcactct gacttcaggg aagagcagaa
    71581 tctttacgga aaaatttata acgcaccact cggaattgtt ggtaataata aagtatcttc
    71641 tgttgaagaa ttatataatc agaaaccatt aatcatagct ttagttttta caagatgtta
    71701 tggcatctgc aatccattct tacttcaatt gagcgaaaat ataaagcatc tacaaagcaa
    71761 ttcagatttt aatattttag ttataagttt tgatccaaga gataaccttc aggatatgac
    71821 taatctttca aagcaatatg gccttgagaa taatcttcaa tggaactttg caataaccga
    71881 tagtattgat gcatttatta attccatagg atttattccg aaatgggata gcgttatcaa
    71941 acagtttgac cacgatgctt tgctggttgg tgtaaatgag aaagggctta taactaaaaa
    72001 tcttttggga atcagaattg tggaagacgt acataaaatg ataagcagca ttaataatgt
    72061 atttactccc tcctaccgtt taccaacaaa aagtaatata ttttcatgct ttaactacga
    72121 tccgaaaaca caaacaaaca gacctggctt aggactttta tttgtagcct tgcctgcaat
    72181 cataactttt cttattgtta caataattgt tattaaatca aagagaacag tttcttagaa
    72241 ttttaaacac actttttgtc acttaaaata aattatcgga actataaaac cataatcaat
    72301 gcaattttgt tttctgatat gaagtttaaa aattaaaaca gttatgtcat cagtaaaaat
    72361 aaaacggatt tatgaagcac cggacaaaag tgatggttat agaatgttga ttgacaggct
    72421 atggccaaga ggtgttaaaa aagaaaatgc tgccattgat gaatggaata aagatattgc
    72481 atcatcttca gagctaagag aatggtttgg tcacgaccct gaaaaattca aagaatttac
    72541 agctcgttat acaaaagaac ttcacaacaa gaatgatgaa ttaaagcaaa tagccggaat
    72601 tgcaaaaaag caaaacttga cgttacttta tggcgcaaaa gatacagagc acaaccaagc
    72661 tgtagtttta caaaaagtat tgagtaaaat gatatgagca ccaaaccaat taagcgacat
    72721 gaggcaatac agtccataag cagagaacat tatcaaggat tactttttgg ttctaaaatc
    72781 aggatggggc ttaaaaaaca aatagagcct gaccgaatgg caaagtacgc tgtttggttt
    72841 tataaagaac atctcattcc tcactttgaa atagaagaga aatatttatt tccggttatg
    72901 agccctgaaa atgaaatgat tcaaagagta cttaaagaac atcgcatgct tgctgattta
    72961 tgtaaatcag aattggaatt ggctacaaac agcaaacttg aattactcga aaaaacattg
    73021 gaagcccaca ttcgctttga agagagagta ctctttaaag aaattgaatc tattgctaca
    73081 tctgaacagc tcgaactgat aaacaaacat cataaagagg aaaagtttgt tgacaattta
    73141 tctgacccat tctggaagta acacctactc ataatttcaa tgaacaacat gttgcactca
    73201 aacatacaaa cagaaaatat tttagttgcc agaaatacac gcccgacaag catgcgtatt
    73261 ttagtttaca atttccttgc aaaacaacaa gctgcattat ctatcactga gattgagaaa
    73321 cattttcagc atgctgacag aattacaatc tatcgtactt taaaaacatt tgaggagaaa
    73381 ggcattatac atagcattca ggaagataat ataaccaaat atactttatg tggatctgct
    73441 tgttcagaac atacccacta cgatcaacat cttcactttt attgtaagtc ttgcaaacaa
    73501 actacctgca aaaccgatat tgccattccg ctaaacaaca ttaccggatt taaaattgac
    73561 gatataacat tttttgcaag aggtatttgc gaaaaatgca ttaacccgac tttgcaatag
    73621 gattgcattg acatctgcca taattttgta ttcagaaaat atatggcatg acgcataaac
    73681 acatttacga ctccagcgga aaacaacttt gttgtactct tgaagataat atttatgaaa
    73741 atgcaggtcg tgttgatttg aaagaaaatc acgatcacaa tgatcatgac catacgcatc
    73801 atgagtccgg tatgttttca atatatctgc ccggattagt ttctttagtg cttcttctgg
    73861 caggaatgca ttttgattat tggaatacct tctcctggtt taatgggtgg attcgtttag
    73921 tttggtatat aatagcctat atcccggttg gatttcctgt aatcaaggaa gcttatattg
    73981 gaattacaaa aggaaatatt ttttctgagt tttttctgat gacaatagct accgttggtg
    74041 catttgcaat aaaagaatat cctgaaggtg ttgctgtaat gttattctat gctgtgggag
    74101 aattacttca gacaatggct gtaagaagag caaaatcaaa tattaaaacc ttacttgatc
    74161 aacgtcctga tgttgcaaca gtattacgaa atagtaacac tgtttctgta gatgctaacg
    74221 atgttggcat tggcgaagta atacttttaa aaccaggcga aaagttagca cttgatggta
    74281 ttttactttc tgataacgga acttttaaca ctgctgcact aacaggagag agtatgcccg
    74341 atgcaaaagt gaaaggagct tcagttcttg caggcatgat taaccttaat actgttgtaa
    74401 aaattaaagt cacagccgct tatgaagata gcaagttgag caagatgctt gaattaattc
    74461 aaaatgcatc tgcacagaaa gctcccactg aattattcat cagaaaattt gcaaaagttt
    74521 atacccctat tgttgttgga cttgctgttg ccatcacttt attgccctac ttttttgctc
    74581 atgagtatat ttttcgtgat tggttttaca gagctttaat atttttagtc atatcatgtc
    74641 cttgtgcatt ggtaataagt attcctttag gatactttgg aggcattggc gctgccggcc
    74701 ggaatggtat tttggttaaa ggcagtaatt atttagatgt acttgcatcg gtgcagcatg
    74761 ttgtgatgga caaaaccgga acaattactg aaggcgtatt taaagttcag gaagctttaa
    74821 tttttccgga agaaaataga aataaaattt tgcaaatggt taatgcgctt gaaaaacaca
    74881 gtacacatcc tgttgcaaca gccattcaca actatgtttc aaatacctca acaattgaac
    74941 tggaaaaggt agaggaaatt tcaggtcatg gaatgaaagc atttgttaat ggcaaagagt
    75001 tgttagtagg aaatttcaag cttttatcct tacataacat atcatttaat gttgatcctg
    75061 ctaccataaa gcataccgtc attgccattg cttttgatag aaaatttgca ggctacatca
    75121 tcattgcaga tagcattaaa accgatgctg ccgctgccat acaaaaatta aaacagatgc
    75181 atgtagatgt tacgatgttg agtggtgata aaacaactgt tgtaaattca gttgctgagc
    75241 aaacagcaat ttctcatgct tatggtgagt tactgcctga ggataaagtt gaaaaagtta
    75301 aatccataat caatcaaaaa caaacagtag cttttgttgg tgatggtgtt aatgatgcac
    75361 ccgttattgc attaagtcat gttggtattg caatgggtgg acttggcagc gatactacta
    75421 ttgaaactgc agatgttgta attcaggatg acaacccgat taaaattcct ttagcaataa
    75481 atattggaaa gcaaacaaag aaagtggtct ggcaaaatat cactatggcg tttgtaataa
    75541 aagcaatagt tttaatatta ggctcaggtg gattggcaac tatgtgggaa gcagtgtttg
    75601 cagatgttgg tgttgcacta ttagcaatca tcaatgcaat gcgtttatta aaaatgagat
    75661 tcgattagta ttaacgaatc agccaataat ataaacctat ttagaaagaa ttaatcagta
    75721 aaaaatgttt ttagttcttt cgaacaacac ccatagaaca gaatttatca attctgttgt
    75781 taaccctgtc agcatcagat acagtaagta aggcacctaa ctgaacatta atttcttttt
    75841 ttacggttgc aaaaatttct tccggattac tatgtgcacc acctgctggt tctttaataa
    75901 tgccatcaat taattttaaa tcgagcatgt cattggcagt aagtttaagc gcattggctg
    75961 ccttttcttt attatcccag ttgcgccata aaatactgga acatgactca ggcgaaatta
    76021 cagagtacca tgtgttttca agcatcatta ctttatcgcc tacaccaatt cctaatgcac
    76081 caccggatgc accttcgcca atgataatac aaataacagg cacctttaaa attgacattt
    76141 cataaagatt gcgagcaata gcttcaccct gtccgcgttc ttctgcttca agtccgggat
    76201 aggcacccgg agtgtcaatg aaagtaacaa tgggcttatt aaacttttca gcaagtttaa
    76261 acagacgtaa tgcttttcta taaccttccg ggttgggcat accgaagtta cgatattgcc
    76321 tggactttgt attgtgtccc ttttgctgac caacaaacat aattgttctt ccatcaacag
    76381 taccaaaacc tcctatcatt gctttatcgt ccttaacatt tctgtcacca tgcaattcaa
    76441 taaaagtatt gtcggtaata gcatttatat aatcgagaga atacggtctg tccgggtgac
    76501 gacttaattg aacacgttgc cacggtgtca ggtttgaata gatttgcttt tttgctacct
    76561 gaattttttc ctcaatctca gttaaaagat tgtttacatc tatttttcct ttattgccta
    76621 tttgttttgt tttttccaat tgttcgtata actcttcaat tggcttttca aaatcaagat
    76681 aattggacat gttttctttt tatttgcaat gacaaaagta gcataatttg attatgtctg
    76741 atgattgtta aaacttatcg aaaatttatc tttgccggca cttatgctta attttccaaa
    76801 tgccaaaatt aatctcgggt tatttataac ctctcgtttt gacgatggtt atcatgcatt
    76861 ggaaacattg ttctatccta tcaactggtg cgatatttta gaaataactg agaataaaaa
    76921 ttatagcaaa tcacaaccta aagtaaattt atatatatca ggattggcag ttgatgggcc
    76981 tactgatcag aatttatgca gcaaagccta tcaccttcta aaagaaaaat tcaatcttcc
    77041 acctgtaaat atatttctgc ataaacaaat accaaccggt gctggacttg gtggcggatc
    77101 atccgatggt gcacacacac tactcctttt gaataaattg tttgatttaa aactgagtaa
    77161 tgaagatctc tctgtatttg ccttaaatct gggttctgat tgtccttttt ttatctccaa
    77221 caaaccatca tttgcctttt caaaaggtga aatattaaaa cctgcaccaa cagtattaga
    77281 gggtaatttt atagttgtaa taaaaccttc tgtttctgta tcaacagcta tggcatacag
    77341 tcaggtcaaa cctgataagc ccaataagag tcttacagaa attttaaact tacccaaaat
    77401 ccaatggaaa gattttctga aaaacgattt tgaaaaaact attttaccac aatttccaca
    77461 aatagaaaaa ctaaaaacta acctctatca ggccggtgct tattatgctt caatgagtgg
    77521 cagcggctct gctgttttcg gattgtttca tgatgaagtt aaattgtctt ttctgaagga
    77581 tgagcagcat cattcagcgt ggctttaatt actttttgcg gccaagcata aaggtgccat
    77641 aaagattaaa aaacacctgc ttatttttgg caaatgccat aataggaata gttttggtat
    77701 aagcatcaag catcacccct acttcaactg cagttattcc atcgttcatg atgttgtatt
    77761 caaacatcac acctgacttt aaaaataagc ctggatatag tttcaactca tccaaacctt
    77821 ttgtaaattc tgccttgcca taaatacttt gtctgtcatg attttgattg ttagggtcat
    77881 atttttcaac cacacgttga ccgtttcctg ttgcaaaaat taaatacaca ggctttgtaa
    77941 accctaatga tggaccacct gttgaattcc atcttatctc gaaatgtctg cgttctgctt
    78001 tatcaaacaa aacacgctca taacaaaatc caccatgcat taccaaaaga gaattcattt
    78061 taccaaatac aaaagtcttt ggtcgttcag aaagatagct gactgacttg tactctttcg
    78121 gatgtttcat gctcacaatt tctatttcgt acatcctttt gcgacttact gtcagatttt
    78181 ttcctttgcg aaacataaaa ccccagccgc tgctgtgcaa ggttgcaccg attgaatatt
    78241 cattggtata acgcaagggc aaatcctcaa cataatcgta acgcacatct tgtgctatta
    78301 caccaaatga taacagaaaa aatattactg tcagccttaa ttgcattgag caaaaataaa
    78361 ttatttttat ttcagccgtt aaatcagggc tttatcatgc tgattatttt ttaaacatct
    78421 aaattactgc tgaaaaacta attttgtatc atgttcagaa agacaaatga ggaaagcctg
    78481 aaagaggtaa ttgagcaact gttggagaca tataaactgc gcgacaaact caatcaggta
    78541 aaactgcaac gcagttggga tgaaattatg ggcgaaatga ttagtaaaag aacagaaaag
    78601 cttattctga aagatcatac attgtatatc tatctcaatt ctgcaccttt aaaagaggaa
    78661 ctcagttatg gtcgtgaaaa aattgttcag atgcttaaca ctgccctcga aggtgatttt
    78721 attaaagagg ttgttatcag ataagtccaa attctaatta gaatgacaaa taaaaaagct
    78781 gcctcttttg aagcagcttt tattttgtga tcccgctggg gctcgaaccc aggaccccta
    78841 cattaaaagt gtaatgctct accagctgag ctacggaatc gggattttta ataacgaggg
    78901 cgcaaaagta ttgttatccg ttttaaataa caaaagattt ttgctttttt attaacaata
    78961 agcgactttt tatgcaaaaa tcggatgtct ttcaaaaggt ttattacggt caaaaagata
    79021 tctataagta attaatgtcc tgctgcgcca tgaccctaac gactctgcca cagataccat
    79081 tttgggattg aaatcgccta tccattgtaa ctcacaatct tcgtacttgg tctcattcat
    79141 aatatgagct gtaccatcta caatcataaa actgtcaata cccttgccct gaaattcagg
    79201 aattacgcca aacacaagcc caatcattct cttagaaact tttcgcttta gcatcaacat
    79261 aaaccttagc ttttcaaacc atccaaattt gccattgaag tatttgaata tttgattgat
    79321 atcaggcaga ttgacccaaa atgctacagg ctcgttttta taatacacat accagattat
    79381 tttttcatca attaccggtt tcattttttt gaaaaattgc tgtacttgct ttgattccaa
    79441 ctccttgccg ctaccatgtt ttacccaggc tttattatag attgttctga aatcttcagc
    79501 aaatttttct aattgcgatt tcttaatgtg aacagcttta aaatccttat ttgcttttat
    79561 caatgcatga cgttcgtaaa acttttcgtc taactgattt cttactttta acccatagca
    79621 atactgcttg aaatatattt caaaaccata agactcaaaa aattcctgat aataggttgg
    79681 attataattc attttatagg gtggctcatg aaaaccatca gtaagcaatc cccaccaact
    79741 gtcacgctca ccaaaattta tcgggccatc cattgcctcc atgcctcttg aaattagcca
    79801 ttcttttgct gtatcaaaaa gtttaaatgc aacttcttta tcatcaatac actcaaaaaa
    79861 gcccatgcct ccggtcggtt gtttattatt cttggtcgca gtttttttat caataaatgc
    79921 tgcaatgcgt ccaacaactt tattactact gttccttaaa atccatcggg tacactcccc
    79981 atgtctgaaa aatttatttt ttgtagcatc aaaaacagat tcaatgtcat tgtctaatgg
    80041 ctgaatccag ttagcatctg aagcataaat ctgctttgga agtaataaaa aatctttctt
    80101 atgtgcggca gtattaactt caataacttg cataggctgc aaaactactt atttttgcca
    80161 tgagcatttt atacattttt ttcattgaaa aataaagttg tcattattac aggagccaca
    80221 tcaggaattg gaaatgcctg tgcacgatat ttcgcacagc aaggatgtca catcgttatt
    80281 gcagcacgca atgaagagaa acttaaccgc attggcaatg agttgaaatc tctttatggc
    80341 aactgtttat ccattaagac cgatgttgct ttagaagatg actgtaaaaa tcttattcaa
    80401 aaaactatag atacatacaa ccgaattgat attctgatta acaatgccgg aatttctatg
    80461 cgtgctctgc tgaatgatgt ttcattgaac gtgatgcatc aggtgatgga tattaatttt
    80521 tgggggacag tttattgcac aaaatatgca ctcccctatc ttttaaaatc acaaggctct
    80581 gtagtgggtg tatcatccat tgccggaaag ataggactac ctgcacgttg tgcttatagt
    80641 gcaagtaaat ttgctatgga aggcttttta gaatctttgc gtattgaaaa cttaaaaaac
    80701 aatttacatg ttttagttgc agcaccgggc tatacggcaa gtaatatccg aaatactgca
    80761 ttaaataaag atggtcagca gcaggaagaa tctccattgg acgaatctaa attaatgaaa
    80821 cccgaaactg tagcttacga aatttacaat gctgttttac atcgcaaacg tgaccttgtg
    80881 ttaaccacca atggcaaact tacagtatgg cttaacaaat tttttccaag ctggctggat
    80941 aaaatagttt ataatttcgt agctaaagaa cccgactcac catttaaata atcaaattaa
    81001 aatgtcaata agaacaatta tattcagtgc actatcctgt attttacttg catcatgcac
    81061 caataccaag cagcagttaa aagatgaaat agactctaaa gaaaaacttt tgtacgagaa
    81121 taaaaacgga cgaattaata caaaactggc tcgcgaagta attattcatt accgctatta
    81181 tgcagaaaaa ttccctgacg attctttgtc agttgagtat ctttttaaag caggcgatgt
    81241 ttctgtgggt attgctgctt atgataatgc attggagtat ttcaaaatca ttacagagaa
    81301 atatccaaaa agtaacagag ctgcctactc actctttctg caaggatata taagtgaaaa
    81361 taatctggaa gacacattaa acgccagaaa atattataat gagtttttgg aaaaatatcc
    81421 caacaatgaa atggctgaaa gcgctcgttt ctcaatttta aatcttggaa aaagtgcaga
    81481 agatgtaatg aagcaaattg acggaaacaa agactcaatt tctgaaaaat aattatgaca
    81541 aaaaacaaga aacagaaaga gagtattgca gaagcacatc agaaaaaaca acagcagaaa
    81601 gctgatgaag caagaaacca taagattaaa attcttcgca ggatacttgc cttaatttgt
    81661 ggattgtgtg gtgtgatatt atatttaaac acattgcatc atgggtttgt attagatgat
    81721 ttttctgtaa ttcttgaaaa ccgcattacg aagcaaggct taaaagcgat acctcagatt
    81781 ttttctacct cctacagaag tggatattat ttaggtactg atgaactgta tcgtcctata
    81841 cctaaatcaa tctttgccat ttcatggaat tggtttggta acaatcctct tccactccat
    81901 ttgttgaata ttgttctttt cggaattacg ggcatcgtgt tgtttaattt ttgttactta
    81961 ctttttccaa atctggtttt ttctttcata gcatcaatgt tgtttatgtt tcaccctgtg
    82021 cacacagagg ttgttgccaa cataaaaagt gttgatgaaa tacttagttt tttgctatgt
    82081 atttctgcat gtatttttct ggcaagatat gttcagacaa aaagaatttc tgaattagtt
    82141 ttaacttcag tctgtttttt tgctgccctg ctttcaaaag aatcatctat tactttttta
    82201 gcagtttttc cgttgctgat tttattgaag tctgacaaaa aggaaacatc atacaattgg
    82261 agtgttgaat taaaacccat gttgacacta acagcatttg cactgttatt tctgatgatt
    82321 cgtcattctg ttctcagtaa gatagcaagt ggtgatccat ctattgcaga caacttgttg
    82381 atggctgcaa aatcttctga tcagaaattt gctactgccg ttatgatttt aggaatgtat
    82441 ttaaagctac tttttattcc tcatcccctt gtttttgatt attcctataa tcaaattcca
    82501 ttggttggta tgggaaacat ctggtttctt ctttctctat taattcatgt cggattatta
    82561 gtttgggcta ttttattatt gaagaaaaat aaattgttgt catttattat tctgttttat
    82621 tttatttcga tgagcatcta ttccaacatt ttttatgtga taggctcatc gtttggagaa
    82681 cgttttttat acactgcaag ttttggcttt tgtatggctg tagcttatgc attttcatta
    82741 ttaagtaaaa atcagaatgt tgaaactgtt tcttcttttt tcaaaaacaa cctgactgtc
    82801 tttctaatat tccttcctgt tgcctttgca tttggctata aaaccatcac cagaaacact
    82861 gtctggaagt cgaactacac tttgtatgct aatgatgtta acctttctcc caacagtacg
    82921 agaacgcact attattatgg caatcttttg gcaaaagacg attttcaaaa agacaaaaca
    82981 gatcaggaaa aagactctat tcaaaaactt gcattgagtg aactagaaaa atctgttgct
    83041 attttcccgt ttggcgatgc ctataatcaa atgggaatta tttatgataa aagaaaagaa
    83101 tttaagaaat cattcgagag ctatcaacaa gcaatgaagg ctgcacctaa cgacccgatg
    83161 attcaaaaca atttaggctc attgtatttc aatatggggc agatacctga ggcaaaagca
    83221 tgttatgaaa aagctgttca attgaatcct tcatactccg aagcgttgat gaatttaggc
    83281 agcgcctgcg gcatgatgaa acaatatgat gatgctgtaa caaactattt aaaagcaatt
    83341 aaatttgatc ctaatctggc gcaggcatat tattatctcg gcttaaccta tcgttttaaa
    83401 ggcgatcagc aaaattctga attctattat aatcaggcag caaaaatgga tcctgaaaaa
    83461 tacggtaaga agaaataaaa aaagctgcct ttgcagacag ctttaagttt gattattata
    83521 ttgcgattac ttatcaatag aaattaattc aacttcaaaa accaatgttt catttggacc
    83581 aatggctcca cctgctccac tttcgccata agctaagttt gcaggaataa acaacttcca
    83641 tttagaacca acaggcatca attgcaatgc ttctgtccat cccggtatta cctgattaac
    83701 gccaaactgc gcaggttgac ctctttcaac agaactatca aatactttac catcaatggt
    83761 tgttccatga taatgtgtag ttactttatc tgtagctgct ggttttggac cggtaccttc
    83821 tttaataatt tcatactgaa ggccgcttgg caatgttacc acacctttgc gtttaccatt
    83881 ctcttctaag aattttttac ctttttctaa gttaacaccg gcttttgctt tcgcttcctg
    83941 atcttttact ttttgttttt cgcccatata cgctccaatg caagcctgaa cttgctccat
    84001 ggttaaggcg gttttttcac ctttcaaagc ttcggaaata cctcttgaaa tcaagtcaac
    84061 attcatgtcg gttagtccgt cttttttcat gttttcgcca atagaaactc ctataccata
    84121 gcttacagag tctaaagtgg ttgtgatggc ttgtgatcca cctgtgtttt tctgtccaca
    84181 tgcgctgaac gccattgcca gaaaactaaa aactgttaca ttaaatttat tcattatttg
    84241 cttttttaaa atccgcgcaa atatagactt atatcatttg caatttcatt ccttagaaaa
    84301 taaaactgag tttctttcat tcacattttg aatgttaata actctgtatg aatagcccgt
    84361 taacttgaca ttttgtttat aattttatga aagtgcaaac cataatgaag ctatttttgt
    84421 gaattgctaa aaagtgaagt attgtactga ttaagatgaa aattacttac tacggtcatt
    84481 cttgttttct gctcgaagtt gcctccaaac gcatactgtt cgacccattt attaccggca
    84541 atccattagc cgttagtatt aagattcaga gcattaaggc tgattatatc ctcatttctc
    84601 atggacatgg cgatcacatt ggcgatgctg ttgaaattgc acgccaaaca aatgctttgg
    84661 taatcagtaa ttttgaaatc tgtaattgga ttcaggacaa aggcatcaat caggtacata
    84721 gtatgaatat tggtgggcac aaactttttg acttcggtaa gttgaaatgt gtaaacgcca
    84781 tccacagcag ttcgttccct gatggttctt atggtggtaa tccaatggga tttttattag
    84841 aaacagcaga aggaaacctc tactattccg gtgatacggc tctaacctac gacatgaaac
    84901 ttattggaga ttataaaaga attgacctgt gcatgcttcc tatcggcaat aattttacca
    84961 tgggaattga caatgcttta attgcttcgg agtttgtaaa atgcgaccgc attattggaa
    85021 tgcattacga cacattcgag ccgataaaaa ttgatcatgc agaagcaaag gacaaattca
    85081 gtaatgctgg taaggaactc attttaatga acattggcag tacaatgact ttttaaaaat
    85141 atatacaata gaaaaaataa aataaatggc taaagtaatt tcgattgcaa atcaaaaagg
    85201 aggagtggga aaaacaacat ctgctattaa tctggcagca agtttggcag tgctcgaata
    85261 taaaacacta ttgattgatg ccgacccgca ggcaaacagt acaagtggtg ttggatttga
    85321 tccacgaaaa atcactaaca gtatttacga atgtatcatc aacgaccttg accctgccaa
    85381 agcaattcag gaaacacaaa cacctaatct gtttttactt cctgctcata ttgacttggt
    85441 tggtgcagaa attgaaatga ttaatctctc taacagagag tatatgatga agcaggtaat
    85501 tgataaaatt tctgctgact atgagtttat tatcattgac tgttcaccat cgttaggatt
    85561 ggtaactatc aatgcgctca cagcttccga cagcgtaatt attccggttc agtgtgaata
    85621 ttttgcctta gaaggtcttg gcaaattgct caacacaatc aatattgttc agagcagatt
    85681 aaatcctaat ctgggtattg aaggaatctt actcaccatg tatgactcac gtctgcgact
    85741 gagcaatcag gttgttgaag aagtgaaaac acattttcag aatatggtat tcagtaccat
    85801 cattcagcgt aataccaaat tgggcgaagc tccaagtttt ggagaaacaa ttattatgca
    85861 cgatgccagc agcaaaggtg ccattaacta cctgaatctg gcaagagaaa ttctgcaaaa
    85921 aaataattta accggtgtac caacacaacg tgaaaaatta ttcaaagcag aagaaactaa
    85981 aaaagttaga atgaactaaa ttattcctta aaccgtttca gattaaaacc ttaaaccggt
    86041 ttcagattaa aaccaaaata tagaatgtca caaaaaaaat cgggtcttgg caaaggtcta
    86101 agtgccttgc ttgacaacag catatcggta aatgaattta aagagattgg tgttgctaca
    86161 tctaatgcgc aaatcacaac cctgcctgtt gatcgtattg aagccaaccc atttcagcca
    86221 cgcagcgagt ttaaagaaga agaattatta aaccttgccg actctatcag agtacatgga
    86281 ctcattcaac ctattaccgt aagaaaactt tctgccgata agtatcagat aatctccggt
    86341 gaaagaagat ttcgtgcagc acaattagtt ggcttaacaa atgttcctgt ttttatccgc
    86401 cttgccaacg atcagagtat gctggaaatg gcattggtgg aaaatattca gcgagaaaac
    86461 ttaaatgcta ttgaaattgc cataagctac aaaagactca ttgatgaatg taaactaacg
    86521 caagaagacc ttggacttcg tgtaggcaaa gaccgtacga cagtaacaaa ctatcttcgt
    86581 ttgttaaagt tgccaccaca aattcaaact gctcttcgtg atgaaaaaat aagtatgggc
    86641 catgcacgtg ccataattag cattagcgat cctgttatgc agttgaaaat tttcaatgaa
    86701 atacttgaaa atgatttaag cgttcgctct gttgaagata ttgtacgagg ctcatcaaaa
    86761 aatgagaaga agcagccaag catcagcgaa aagtttatca aaggaataga gtacaaacat
    86821 attcagaaac gtttggaaga taagtttgaa acgaagattg aagttaaaca tcgtaaaaat
    86881 ggttcggggc aaattatgtt tacctatttt tctactgacg atttaaatcg gttactcgat
    86941 attcttgacg cataaatttt tagctgcatg aggttgagat tattggttgt tctgtttttt
    87001 acggtttact gcgtaaatgc ccaacaggac accatgattt ttaagaccag aacaaatgtt
    87061 attgttaagg ccgattccat aaaacccact tctgatttat ctcaaaaagt caataaacat
    87121 tctccacata aggctacaat ctattcagct attttaccag gattaggtca ggcttacaat
    87181 aaaaaatact ggaagatacc tgtaatttac ggactaacag gagtgcttac ctattttgct
    87241 attgacaaca acaaagagta tgtagtttat aaagatgcct ataaatggcg gctcgatgac
    87301 aacagtgcta ctgtagataa gtttgaaggt atttactcag atgaggattt gcgaatttta
    87361 aaaaactatt atcgcagaaa ccgcgacctt tcctttatcg gaatgggtgt tgtttatttg
    87421 ctcaatgtag ttgatgcagc agttgatgct cacttgtttt attttgatgt aagcgatgat
    87481 ttatcattaa aagtgcagcc cggcattcaa ccaatgagcc atactgcatt tgcaggcgta
    87541 aaactgagtt tacaaataaa gtaagatatg aaaattgcac tgttaggata cggaaaaatg
    87601 ggcaaggaaa ttgaaactgc tgctattgag agtggacatc aaattgttct aaaaattaac
    87661 tctgccaatt acaatcagct taataaggag tctctacaaa ctgccgatgt tgccatagaa
    87721 ttttcaaagc ctgaagctgc tatagccaat gctaaattat gtgttgattc aaacgttcct
    87781 gtaattatag gtacaacagg ttggtatgaa cacaaagcaa cattagagca atattgtatt
    87841 caaaataatg gaacggttat ttatgcttct aacttcagca cgggtgtaaa tgtttttttt
    87901 atcattaatg agatgttgtc taaacttatg gcgcctatga aatatgatgc atggattgaa
    87961 gaagtgcatc acattcataa aaaagatatt ccaagtggca cagccattac actagctaat
    88021 gatgtactga aatataacaa acaatattca ggatgggcaa ttaatgatga agatggtaaa
    88081 cttcccatat acgcacagcg cattgacgaa gtaacaggtt ttcattcagt ttcattccgt
    88141 aacgaagttg acaccattga gattacacac aatgcattca gcagaaaagg atttgcactt
    88201 ggtgccataa aagctgcaga aatagctttg acaaaaaaag gattttacga gtataagaac
    88261 cttttaatag aacagccttg attttatgtt gctaaaagag cgatataaat atgtaatcag
    88321 ctacttcagg aaaaacatgc ctgtggcaga aactgaattg gtttacaaaa atccatatca
    88381 acttttagtt gcagttattc tgtcagctca atgtactgac aaaagagtca atatggttac
    88441 tcctgaatta ttcaaagctt ttcctacggc taaaaaactt tcagaaacag agcctgaaga
    88501 agtttttgag tatataaaat ctataagcta tccaaataac aaggcaaaac atcttgtcgg
    88561 catggcgcga atgcttttag aaaagtttga aggcattgtt ccatcagaac ttgatgatct
    88621 tattcagttg cccggtgttg gaagaaaaac tgctaatgta atttcatctg ttgttttcaa
    88681 taaagccgca atggctgttg acacacacgt ttttcgtgtg gcagcacgat taggtctgac
    88741 acgcaatgca aaaacaccat tagctacaga aaagcaatta gtaaaacata taccggaaga
    88801 actaattgca actgcacacc attggctcat acttcatggc cgttatgttt gtacggctcg
    88861 aaatccaaaa tgtaatattt gcggattaaa agatgtttgt gtttattata aaaaacatcc
    88921 tgtttaagat aacactcctt tgccttcgcg cacaacagta atttcatctg ttgtacaatc
    88981 tacaattgta ctcggcacgt tccctcctgc tccacaatca acaatcatat caaccatatc
    89041 gctgaacctt tcataaattt catcagggtc ggttagataa tctgtaattg gatcatttac
    89101 atcatgcagt gaagccgaca tcagtggatg ccccagttgc tgaatgattg atagagttac
    89161 tttctgatca ggaatacgaa taccgatatt cttttttctt gccctgaata taccgggcac
    89221 ttttgttgat gcttcaagaa tgaaagtaaa tggcccgggc aatgaacggt tcaataattt
    89281 atagatactt ctatcgaaag gcttggtgta ttctgcaatg ttttttaaat cattacataa
    89341 aaaagaaaga ttagcctttt caggttttac acctttaaaa cgacttactt tctcaatggc
    89401 aggcaaactg taaatactgc atgccaaagc ataaactgta tcggttggta taatgatgat
    89461 gccatcattg ttgatacatt caacaacctt cttgattttt ctgtcatcaa tattattctc
    89521 gttaatttct atcaacattt tacttttgtt ttactgacaa caaaaaaaca acaataaggt
    89581 taatctttcc agtcagcaat aaacaaattg gtgtcatgtg ttccaccatt atttctgtta
    89641 cttgaaaaaa ttaatttctt gccatcaggc gaaaacatcg gaaatgcatt aaacacactc
    89701 tgatcggtaa tttgtactaa acctgttcca tcagtattaa tcatatacaa ctgaaaatta
    89761 aatcctgatt tcgattcatg attggatgag aaaagaattt tctttcctga tggatgaaag
    89821 aaaggtgccc agtttgctct accaagatga gtaatctgtt tcagattgct accatcaaca
    89881 ttacaagtat agatttccat attggttggt gcaaccaaac cttcgcttaa caactgtttg
    89941 tactcttgaa tttcttcagc tgtagttggc cttgatgagc ggaacaccaa ttgtttgctg
    90001 tcgggtgaga aaaatgcacc cccatcataa cccaaaccaa acgtaatttg cttctgattg
    90061 cttccatcaa tatccattgt ccacaattca agatcacctg tacgatcgct agtaaaaaca
    90121 atctttttac catcgggcga aactgttgct tcagcatcat atcccggtgc attggtaagt
    90181 tgtttcacta tttttccatt caggtcagca acaaaaatat cgtaggtatt aaaaatcggc
    90241 cataaatatt ttccaccacg tctaaggtca cctgtttcgg gacatttatc tcctcttaaa
    90301 tgcgtgcttg cataaagaat ggttttatta ccgggcataa aatatgagca ggtggtacgg
    90361 cctttaccgg tactgattaa tggaggtttg ctatcggatt tccagttatt aagatctaat
    90421 aaaaatatct gatcgcattc aacaccccat gctttattgt tagattgaaa agctaattta
    90481 ttgttgtcaa aactaaaata agcctcggca ttatcacctc caaaagtcaa ttgtttaatg
    90541 tttataagat gtttattctc aatagtatca acatcttggg ctaatgcaaa gtttaagcta
    90601 aaaggcaaca gaattaacag gtacttaaat cgtttcataa ttttaagata ttttgtcgcg
    90661 aaagtatccc ttttgagagt gcaacttggt gtgatttttg tcagtaatgg agaaaatgac
    90721 gaaatcgtta caagaccctt ttgccgatat ttttctacaa aaatctttgt tttatgattt
    90781 ccttaacata tctttgaagt tactctaaat tgaatgataa tgaaaaaact tttcactgta
    90841 ttgactattc ttctgatagc aagcacaaat caggcacaaa atgtaggaat tggcaccaat
    90901 gttcctatgc aaaaattaga tgtgattggg aaaattagat catcaacact tactggtgtt
    90961 ggtaatcgtt ttgtgatggc agaccctaca ggtacactaa atgtgagttc aagtggttta
    91021 cttgattcaa cagcatggct aacacttggg aatagttcaa tagatactac aaaaaatttt
    91081 ctcggcacat taaccaacac acatatatta tttaaaacaa atggcaacgc tgctgcaaat
    91141 gaaagaatgc gcattcgttc aggaggagaa attatagtta ataatacttc tgtaggtaat
    91201 aataacggtg atgtgttttc tgtttatgca acaggaagta gtaatggtat caacaatgca
    91261 ttgagcccaa ttggtgcatt tgcactaaat ggttactctg caacgacagg cattggagta
    91321 tctggtgcta acacaggaac cggtgttggc gtttggggca gttcatcttc cggttatgga
    91381 actgtaggat tttccggagg taatacagca tttgcaggaa tgtttcagaa ttctaatgcc
    91441 aatggaacag gattacttgt tacaggaaat aatcttccag gaacatattt agtaaatggt
    91501 tctgctggtt ctttccgagg caccaatttc ggcattatta atttttcaaa catcagttct
    91561 gctgctgttg ccggtagtgc agtttgggga agtaatagaa aaacaatcat aacaagttat
    91621 ctcggtggtg ccggtgtaac tggaattgat tctgcatatg gatccggtgt tataggtgtt
    91681 tcattttttg gaggcaataa tggcgttaca ggtataggta gttctaatag tggaaccggt
    91741 gtaatgggtc aggcagggat gggccccaca ccctatgcag tatggggaga tgtaacaacc
    91801 gtttcaggaa caaatgctac ctcaattgct gttattggaa gtaataattc agttggtgcc
    91861 aatgcaattg gtattttagg acaagaagtt gctgcaccca atggtcctac acgttatgca
    91921 atatttgcta acggtgattt agccgcttca ggaacaaaaa cttttgtaat tgatcaccca
    91981 ctagatccat caggaaaatt tctgaaacat ttttctattg aatcaaatga agtacttaat
    92041 tattacagag gtaatgttgt actcgatgca aatggtgagg caactattca actaccttct
    92101 tatttcaata gtataaataa ctcaaactac aactacaatc tcactcctat tggtgcacct
    92161 gcacaacttt atgttaaatc ggaagttaca ggtaataact tcgtaattgc cgggggcgtt
    92221 gccggaatga aggtaagctg gacactaacg gctgaaagaa acgatcctta tctgcaacaa
    92281 aatccagata gtcgtttggt tgaacttcca aaaacaggag acgctgcagg aaaatattta
    92341 gttccatcat tgtatggaca accggagtca gcaggaattt attacaaacc tattcctcaa
    92401 aaaactgctg taaacgaaaa atcgaaagat gcaccgttaa ttaaatcaag agattttaca
    92461 caacaaaacg attctaaata acaagctcat ttcatcatta aaaactatat gagtatgaaa
    92521 aagtttgttt actatatttt attatttctg ctacctgtaa cagcatggtc gcagtgtatg
    92581 atgacacctg tttctttaca ggacagaatc agtaatgcaa ctgttgttgt tgaagctact
    92641 gccttgcaac aaaacagcta ctggaacaac agtaatgatt tcatctacac ttccactaca
    92701 ctttcaattt ctgcagttta taaaggcaca attcagggca gtcagattgc attgattaca
    92761 gaaggcggta ttgttggtaa catcatgatt aagtcagaac catctttgaa tattaaaccc
    92821 ggacagaatg gaatattttt tctaattcca cacagccagc cgtcaggaag taaactgtca
    92881 ggaatgtttg aaggatatgc tttgagtcag agtttaattt tgtttgatga aacaagtaat
    92941 tcagcaacag gtccgtttga aaactacagt gacatcaatg cattacgaaa tcagattatc
    93001 aatgctacag gtggcgttca gcgcatttct tctattgcac cacagcaggc acctccatct
    93061 gtaaataaaa taatgattcc gccaactatt gcttcaatag taccggcaac aactacggca
    93121 ggaacatttt caacaatcac aataaacgga aataattttg gtgccaccta tgttaacgga
    93181 acttcaaacg ttgaatttcc tgatgccaat actggaggtg taagttatat ttctgctccg
    93241 caaaatcata ttcagtcgtg gagcaacaca caaattcagg tttgggtacc tacacaaggt
    93301 ggaagcggat taataagagt aacaaacaat cttggagaaa ctacaacttc tgcaatttca
    93361 attaccatta attataatga aacaaatgtt gttagtggta gtaattatta tcaacccgac
    93421 ttgattaaca ataatggaac cggaggttat acttttcgtt acaacacaac atttaatggt
    93481 aatgctgctg ctgttgctgc atttgaaaga gcattacaaa catggcgctg tggaactttt
    93541 gtaaacttca accgctcggg tactactgca acaacttgtc aggctttaga cggaactaac
    93601 ttagtgacct ttgatggttc atgtgcattg cctgccggag ttctcggaat ttcgtacagt
    93661 tattacagtg catgcgcaac aggcgtttgg tatcttaacg aaaacgattt aaagtttcgt
    93721 accaatggaa ccggaggcat aaactggaac tatggccctg ctgcaaccgg tggtggcaat
    93781 ttcgattttg aatcagtttg tttgcatgaa ttaggacaca gtcatcagtt aggtcacact
    93841 attgtgcctg tgacagtaat gaattatgct attggcccaa atacagacag acgtacactt
    93901 acaccaatca gtgaaactgc cggtggaaat gatataatga gtcgtagtgt ggtgactaat
    93961 gcctgcggac caacagctat ggttgcgctt tctgcgggaa gctgtgccat caatgcacct
    94021 attgctgatt ttaatggtac accactgaca ggatgcaata cgctgaacgt aacttttaca
    94081 gatgcatcca tcaatactcc aacatcgtgg acgtggtctt ttccaggagg ggtaccaaat
    94141 acttttgtag gacagaatcc tcctgtaatt ttttatgccg caccgggcaa ctacgcagtt
    94201 accctaactg taagcaatgc atttggaagc aatacactta ctaaaacaaa ttatgtacag
    94261 attgcaacct gccctccacc tacacctgat tttacagcta atccaacaac cgtttgtcag
    94321 ggaaatactg tacagtttgt agatttgagt accaacacac caacaagctg gagttggact
    94381 tttccgggag gtacaccggg aacatctact gcacaaaacc ctgtgattac ttatgccact
    94441 gcaggaactt ataatgttac attaacctca actaatgcct atggcagcaa cacattaaca
    94501 aaaacaaatt acataacagt aaacaattgc cctgctccac cggtcgctga ttttagtgca
    94561 tcgccaacaa cactgtgttc cggtggtaca gtaaacttca ccgatctttc aactaacaca
    94621 cccacatcat ggtcgtggac atttgccggt ggcacaccgt tatcatctgt agcacaaaat
    94681 ccaacaataa cgtataacac acccggcact tacgcagtta cattaattgc aactaatggt
    94741 ggtggctcga acacaaaaac tgtatcagga tatattacgg taaatctttg ctctgcacct
    94801 gttgcatctt tttcaggatt tcctacaacc atctgcgaag gtcaggctgt gacttatgta
    94861 gatcagtcaa ccaactcacc atcatcatgg gcatggactt ttaccggtgg tgcacctgca
    94921 gcatctgcct tgcagaatcc tgttattagc tatgctactg caggcaccta cactgtttca
    94981 ttaactgcaa caaatgcttt tggcaacaac acttttacta gaacaaatta catcaatgtt
    95041 gttacatgtc cgtcacctgg aacaggactt attgtcaatg acgggtcact gatttttgtt
    95101 caacctactg caatgatgca tgttgaaggt ggtgttatta atcaggataa cctgagcaac
    95161 attggtacat gggataactc cggtcaggtg cgtatacaag gcgactggac aaacaattca
    95221 tctggaaatg cttttatcaa cagcagcccg ggtgaagtgg aactgtttgg cgccaatcaa
    95281 actataagag gtagtacacc tactttcttc tttaccttaa atctgacagg cacaggtata
    95341 aaaacccaaa atattgatgc aacggttcag ggattgctga agttgaacga cagagaactt
    95401 gccacacaag gaaatgtgat gcatgttacc aatacagcac cggcagctat tacgcgtaca
    95461 ggtggactta actcaactcc tgttcagggt tttgtgagta gcacaggcag tggtaagttg
    95521 tggcgaaaca ccaacactac atctgtgtat ttatttcctg taggttcttc aatacctccc
    95581 gcacgttttc gtccagttga aataaagcca ttgagtgcaa cacccaatac ttttgctgta
    95641 cgtatggtta ataatgaccc gaatactgat ggttttaacc gtgctgtacg tgaacccacc
    95701 ttaggtgcag taaatgcagg atggtatcag aaaataaaca gagtttccgg tagttcgtta
    95761 gcggagtttt cgctttatgc cgatgatgca caggatgcca ttgcttcatg gccattactc
    95821 aaactttgtc agtggggaca ggctgcccct cctgtatggt ggaaagatat tggcggtgct
    95881 gtaactccgg gcgcttcacc tgtgttagga tcggtgctgc gtaccaactg gaataatttc
    95941 agtaatacag aaaatttcaa tattgcacca gaatcaaatc cactgcctgt tgaattactt
    96001 tcgtttaccg gtaactgtaa gaacaacatt gttaatctga attggacaac tgcatctgaa
    96061 ataaacaatc attattttga attgcaacaa tcagccgatg gaaatgattt tgttactata
    96121 ggtcgtgttg aaggtaatgg taccaccatc aaacaaaatt tttattcttt taccattgat
    96181 caaaaaactg aacacacatt tttccgattg gtgcaatatg attttgatgg aagagctaat
    96241 gtattgccgg ctattttagt ttcgtgtaac acagaaagtc ctgcaacagt aaatatcaac
    96301 ctgatgccca atccggcaaa agatgttttt gtttctgaca tatatcttcc tgaaaaaaca
    96361 caactctcca tgaaggtatt taataccatc ggacaattgg ttatggaaga acaatggaca
    96421 cttgatgcag gctatcataa acaagcaaca caggtttcta ctcttgcaaa agacatttac
    96481 aatatccgct atacttcaaa ctcgtttaac atagtaaaac aaatagctat tgtaaaataa
    96541 ttgctgatgt cagaaattgt attgcgtagt ttgcaaattg acgatgcaga aatatttgct
    96601 ttgcatgcca acaacagtgc aattgcagat aacatgacca atcagtttcc ctttccatat
    96661 acgaaagcac atgcactgga atttatcaga agagttaacg gttttaaacc tgctcaggta
    96721 tttgcaattg acttagatgg agaggcaata ggtgctattg gactacaccc gcagactgac
    96781 atttttttaa aaaatgcaga gcttggatat tggcttgctg aaaaatattg gggtaaagga
    96841 tttgtttcca ttgctattaa aaaaatagtt gaatatggtt tcagcagttt tgaaatagat
    96901 agaatttttg cacgcccctt tggcagcaat aaagcttcgc aacgcgtatt ggaaaaaaca
    96961 ggatttgttt tggaagcccg ccttgaaaaa atcatttata aaaacaatcg ttatgaagat
    97021 gagttgattt atgcaatcag gagaaagtga ttaagtgaag cgcgatgaag tgaggggtta
    97081 tgatcaacaa cgactgacaa ggggtgatta agtgattatg tgaaacgtga tgaagtaaag
    97141 tgaaaattca gaattcagag tgcagaattc agaattgaaa aaagtgaaaa taacatcagg
    97201 cattaaattg tgattagcca cggctgacag aggtgattag gtgtaaggac aatagatatt
    97261 agcataagac tttagcctga tggatagaaa agccaatccc ccaaaaaaaa tccaccgatg
    97321 gtttttaaca gcggtggatt ttttatttgt ggactaaagt ccgatatgag gcttagggat
    97381 catctatctc aggctaaagc ctttggtaga attcaaaaac ttatctcaac acataagatg
    97441 atgccccgct gaggttgcca tcaatataca cttctatcag ataggtgccc ggtgtaaata
    97501 ttctatcgtt ctcctcataa ctaaggcaat tgtcaacggt ggcattttcg tagcgcagcg
    97561 atgaaacaac agtacacatt acctcttcac cactatccat tttaaaagat gtagatccgg
    97621 cagagcggtt acccataacc ttaccacccg gctcaatgat tctgagataa acattttttt
    97681 ctcctgcttt ggcaatttta ttttcaagaa cagaaaaaca cacatccatt ttattggtac
    97741 gttttgataa agcggtggac acaaatttgc cattgccttt tttcttgaaa gtagaaactt
    97801 taaaatattc tgactttaaa accgatgcag tgttaaccgt tgtttctaaa tttttagaaa
    97861 gcgtttcgac agtgcctgat aattgtgcat tatcatcttt gagtttttga ttttctacca
    97921 tcagggtatc tatcttatcc aaatattcat cacgcagttt tttgagtgac tctaattctg
    97981 ccatcagctt tttgtttaat gctgcgctgt tgccttcgct tcttattaat ttctgaatgc
    98041 gctctttctg ttcatcaatt ttatgattgg cctcggttaa aagactgtcc agattgtcat
    98101 tgataccttt gtacttgttt agctcttcga tggtggcacc caattctttt tcaacgtcaa
    98161 cacgtgctgt aatgaggctg tctttagtaa cctcaaatac ctctgtagtt gatgtgtgat
    98221 tgcgccattg atatacgtta aacaatgccg aagcagcaaa taaaacaatc caaatacgat
    98281 tattcccttt actctggttt tccatgataa tatttttata gattaattaa caaaggtaat
    98341 atgtatttta aagtaaacaa ctcctgtatg tcaaggtttt gaacaggtac aatgtcaaca
    98401 aatgggcata aagtcagaac ccgaaacggg cacttggggc cacatcaagt gttgaaaaat
    98461 cagcagcaag atatttgaaa tatgcagcaa gacaaatcat accgccattg tcagtacaca
    98521 gcgaaagtgg cggaatatgt acctgccaat tatttttaac tgcctcttct gttacggctt
    98581 tccgtagtcc gctgtttgct gatactccgc cagcaatggc aatattgctg atgcctgttt
    98641 ctaaagctgc cttttttaat ttaagtatga gtgtttttac aatggtatat tgcacggaag
    98701 cacataaatc atctctgttt tcagcaataa aagcaggatt ttgcgcaagt tgtttcttta
    98761 aaaaatacaa gacagaagtt ttcagtccac taaaggaata attgagcccc ggcaggtttg
    98821 ccacaggaaa attaaatgct tttggattgc cactttgtgc atatctgtca atgagcggcc
    98881 ctccgggata gggcaactca aatactttgg caattttatc aaatgcctca cctgctgcat
    98941 catcgagtgt ctgcccaatt atttccattt gctgtggtga agttactctt acaatttgtg
    99001 tatggccacc cgaaactgtg aggcataaaa aaggaaatga cggagttttg ccctcctgcc
    99061 cttctaaact taaaaagtta gcaagaacat gtgcctgcat gtggttgact tctatgagcg
    99121 gcttgtttaa tgcagcagca gcagacttgg caaaagaaac accaaccatt agtgagccaa
    99181 taagtcctgg tccacgagta aaggcaatgg catcaacatc gctgatattt attgccgctt
    99241 ctcgtaatgc ctgatccact acaggaacaa tgtttgactg atgtgccctg ctggctaatt
    99301 caggaaccac accgccatat ttctgatgta cggtttggtt ggcaataaca tttgataaaa
    99361 tctgtgtatt gcgacttacg gcagcagaag tttcatcaca ggatgattca atgccaagta
    99421 tgattatgtc ttttttatca gtaaccgtca ccttctttta cactaaaaat cagtcagcaa
    99481 agttaagagg aaaaatcagt ttgtctgaca ggcttcaaaa cacatcttat taaccatcta
    99541 taatgcaatg aatttagggt aaactaacaa gagtgtggca ttatttttaa ggcaacattg
    99601 gttaatccac ttaaataatt acactatttt tatccgcttc aaaaacaaca ggtcatcagg
    99661 aaatttttaa aaatattact tattacaatt ggcacattgt tgctgctgct ttttttgttt
    99721 tctgtcagct tacgatttca aaaagttcaa agttgggttg ttgatcgtgt aacaggatat
    99781 ctatcatccg aattaaatac aacagtacat attggcgggg taaggtttgt attgataaaa
    99841 agtgtatatc ttgatgatgt gtttattgcc gacaaacagc aagatacgct gttgtatgcc
    99901 aaacacctta cagcaacagt ggcttttcag tccttgttta tcaggaataa aaaactgatt
    99961 cttgatgaag tgcagttgga caaagcgcga atagcattat caaagtcgaa gagcacaaga
   100021 gatatgaact tcgattttat tatagattat ttcagttctc cttcaaaaga cactacatca
   100081 tcaccatttt attttgccgt gaatagggtt attttaagca atatacattt cacttatcgc
   100141 gattttaagt gggatgataa aacaccttgt attgattttg aagatattga cgtgtcagac
   100201 tttaatggaa cactttacaa tataaagcca acagacagct cgttttcatt ttatggaagt
   100261 aaaatatctc tgaaagaaaa atcaggattt aaaataaact ctttttctac ttatgctgct
   100321 ttttataacg acagcatgtc gtttaataaa attctgttgc gcacacccta ctcttctatt
   100381 gatgccggac actacagtat gtcgtataac agtatgacag actttgaaga ctacatcaat
   100441 aaggtaatga tgaaaggcga gtttaatgaa agcacaattt cttcaaacga cattcagtac
   100501 tttgccgaag aactttttgg cctagataaa cgtgttgagt ttagtggtac tgccagagga
   100561 acggtgagca atctaaaaac caaaaaactt aaaattgctt atggaaagag cacacgattt
   100621 gaaggtgatg taagccttaa tggtttgcct gatgtaaata caacttttat aaatctaaac
   100681 ataaaacatc tttcaaccaa caagaccgat attgaaacca ttaaacaata tccatttaca
   100741 gagcaacaat atattaaatt gcctgataac atttctgaac ttggtaagat tgtttacaga
   100801 ggtgttttta caggatttta taacgacttt gtttcgtatg gaaatattac aacagcaatc
   100861 ggcaacctgt caaccgatgt aaatttaaaa ttcaaaaaca aggcaaatga gtcaacttac
   100921 tccggtaaac tcactgccga caactttgat attggtaaat tctggcgatt gggaaatgcc
   100981 gtaggaacaa tcagttttaa tgcacagcta aatggtacag gatttacatc agagaaagtt
   101041 aatgcacgtc tgacaggttt ggtaagtcat gttaatttaa atggctatga ttattcaaac
   101101 atcaacttag atgctgatgt ggcccggaaa ctttttaacg gaacattaaa catgaatgac
   101161 agaaatgcac aggtttcatt taaaggaagt attgatttca gagagaaact gcctttgttt
   101221 gattttaatg ctatagttaa aaatgccgca ttgactaaac ttaatctgct aaaaagagat
   101281 acatctgccc tactttctac tgaagcaaat ctgaacatgc gcgggtttaa ttttgatgag
   101341 tttgagggaa aactgcaact taaaaatatt agttattcag aagaatataa atctgctttt
   101401 gtagaaaaaa tattgattca gtcgggcatg gagaataata acagaaacct gaatgtgaat
   101461 tccgatttac tggatttgaa tattaatggt cagtatatat tatctaaaat tgtttattca
   101521 ttcggtcatc tacttaactg ccatctgcca ccattcatgc ttaaattatc acctgatgta
   101581 gaaaaaacta atactatttt ttcattcagt ggaaatataa aagatgtaaa tcctgtgttg
   101641 aacatcttta ctccgggatt gtctgtaagt aatcaaactg agttttcagg aaggctgaat
   101701 cgtcagcatt gcgaaataga agtggatgca acatcaccct ggattaatta caaaaatttc
   101761 gaaaataaaa acattaaact cacatcaaac aataagaaca atttattaaa tattgattta
   101821 tctgcctctc aggttaaaat ttctgacagt ttaattctga aaaatatttt acttgatgca
   101881 gagacacaaa aaagcaaatc atccattgta cttacagttg ccaacgattc actctctacc
   101941 tcaaaaatga gactggcaac agagcagact tacacaaatg gagcgacacg ttttcatttg
   102001 cttgagtctt tgcttcagtt caaaggcgaa caatggcagg ttgatccggc aaacgaaata
   102061 ttattcgact cagcatctat ttcatttgac aaggtgttgt tcaccaacaa aagtcagtcg
   102121 cttatgttgc agggcaaagt aggaaacaat aaagaagatg tgttgagtgt gaacttattc
   102181 aaatttaagg ttcctttaat aaatgactta ctggctatgt atgatatcaa gttaggtggt
   102241 gctgcaagtg gtaatttgaa tttcagttca ctctatcaac gagcaatcat caccggtgaa
   102301 atgcgagtgg atagttttgc gtttaatgac gaaccattgg gaaatgccat cgtgaatttt
   102361 gattggctga cagaccaaaa gaaattgcat attgaaggtg ccattgaaca aaacaataga
   102421 aacagtgctg ttgtaaaagg tgactatatt tttcaaccaa aaaatgatga gctgagtttt
   102481 aatattaaag ttgacaaact tagcataaca cctttagaaa gataccttaa tggttttgca
   102541 agtaaaattg taggacttgc aacaggagag ttaaatctta caggattggc aaaagcacct
   102601 aacctaaccg gcaaggcccg attacaacgt gcatctatgg taattgacta tctgaacaca
   102661 cgctattctg tgttgccgga gcaggatatt atttttgatg aaaaatatat tgcatttaac
   102721 aaagttggaa tttctgatga tcagaatcac aaaggcattg ttgatggaaa aatatttcat
   102781 aatcatttta atgatttcac tttcagcctt agagttgatg caaataaact tcaatgtctt
   102841 aacacgaatg cttctcaaaa cgaattgttc tatggcaaag cttttgcaac aggttatgca
   102901 aaaataatcg gcacgttaaa cctgatgaaa ttggatgtag tggcacgttc tgaatcgggc
   102961 acacaaatat tcattcctct ttcaaaccct gaagaagtga cacagagtaa ttatatcaac
   103021 tttatcaatg tgaatgatac tgctgcaaaa aagtttgtgg taactaacaa agttgatttg
   103081 tcaggcatac aaatggattt acagcttgaa gcaacgcctg aggcagagat tcagctgata
   103141 tttgactcaa aagttggtga tgtgatgcgt ggtaatggat caggaatttt aaggatgcag
   103201 atttcgcagg caggggaatt ttccatgtat ggtgattaca atattcttgc aggtgattat
   103261 ttgtttacac ttcaaaatat agttaacaaa agatttaaaa ttcagagcgg gctgatacaa
   103321 tgggcaggct cgccttacga tgcaaccatc aatatcaatg caaaatacaa ccttcgtgca
   103381 tcaacgtttg atctcattcc tgactcctca aaccgcagca gagtaccggt ggaagtattg
   103441 ttgcagttaa agaacaactt aatgagccct gatgttattt ttgacgtaaa tgtgccaaat
   103501 gccacacctg ctgtgcgaac aagtctgagt agtattttag gtaatgagcg cgaaatgaac
   103561 aggcaggttt tcagtctgct tgtgctaaat cggttttcgc caccggaagg aaaaaacacc
   103621 tctgaagaaa gtggttccgg caatgcagtt ggccagaatt tgagtgaatt tgtttcgcag
   103681 cagctatcga attgggcatc gcagttgagt gataaattta atatcggagt taactatcgt
   103741 ccgggcgatg cacttaacag agatgagttt gatgtatcta tttcaacaga attgttcaga
   103801 aacagagttg ctgttgaaag taatgttggt gttgccaaca acagcaatca gacttcaagt
   103861 attattggcg actttcaggt tgaatttaaa atcagcaaag acggcaatct tcgtgcaaaa
   103921 gcgtttaata aaactaatac aaacaatctc atcaacaacc ttaactcaca atacactcag
   103981 ggaattggtg ttttttacag aaaagaattt aatactgttt cagacttagt tgaatcgttc
   104041 agaaaaagaa aaaaagctgc tgcaccaaac taaatttcca tcagcgagct gcttcctcct
   104101 acccaggttt tattcttatt gtggcttaca ccaatcattg cacctttact taatcgtgta
   104161 agattaagca ccaactgcgg tttggtttca gcatcatccc agatataaag caaacgaact
   104221 tccacctttg cagctccatc gggtgtttcc atgcaaggca cataatttac ttttcgctgt
   104281 attaaaaatt catttctttt ttccaatgga atgctttcaa tgtctgacga tgttacatct
   104341 atcaacaccc cactacctgc aaaggaaaat agcggcttta acacaaaatc agcaaggctt
   104401 aattttgatg tgtcaacatc acttaaaaaa taacattccg aagcaaatct gcttttaata
   104461 aaaggcatgg tgtatttact gattctgaaa aaccagtttg gatgtcctgc ccattccaca
   104521 tctacatcat cagtcagatt gaaatcttta atcaggtctt ttcgttgcag caactcatca
   104581 aaaataattc tgttataaat tctgtaaatg ggagttttta ccccattcaa ttcataaaac
   104641 aaattttttc cttctttaat cactttcgat atacaaaccg gttctactcc tgtatgtgtt
   104701 tttgtttccc aaaagtcaat ccatgttttt tgtttttcgg gttctatttc aagcaagata
   104761 acattctccg gtttatgatt tccaagaata actttacgca tcaatgcacg atgcttttca
   104821 gtattccatc catcaaaaaa atgactgaaa ccttcaggta tctgaaaata tttggcataa
   104881 gtgcgcggca acaaatcctg ccagaaaaaa agagaggcaa acccttgcag ttctatcaat
   104941 tgcggcaaat aatttccatt atcatctttg cagatggcaa aatcaattgc aataaaatgt
   105001 ggatgcttat gttcattcgg aacttttaat ccatgcggaa tagcttgttc actttttaaa
   105061 agatattcag gagtcagtga ttgagataat atttcattgc cggcttcaat gagtaaatct
   105121 tttaattttt ttggaataaa aactggcgac tctgcaattc ggaattctac ctttctgttg
   105181 taaaggttat cataataagc taaaaattcc gagtatgttt cttccttaaa ttctcttaaa
   105241 tatttatctc taatttcttt aatcatgtgt caaaaataaa aaagggtgac ttatttctaa
   105301 atcacccaag taaaataaat ttccgtttaa agttttttta tctctctttc tgtaacccac
   105361 ctatctttaa aaactgtttt atcatattta atatgatatt tattttctcc aacttcaatt
   105421 acagttgcag gttcaggaac cataccatat tccacttcaa ccctatctcc aaccttaaat
   105481 tttgtcaatg gttgtgcagg tgcttctttt gcaaaatctt ttaaacgatc aatcgtaacc
   105541 cactcgttcc aaccttcatc ccaaccatca taagaaacat aataggtgtg atcatctgca
   105601 ttaattttca aaattttacc gggataccat gcgccttttg aatttatttg tactttctcg
   105661 ttaacttttt gcgcataagt aaaagttaaa aaccctaaaa agaacaaact tgtgaatatt
   105721 agttttttca ttttatttaa ttttccccaa atatagtatt ttattcaggt taaggaggcc
   105781 attctaccgc taatttataa acctcataaa aaatttatct tcgtgtatag aatcctacca
   105841 gtccgaatca aaattttgtt tgatttgcat actgtaaaat ggttcaggaa aaacgggctt
   105901 taatctttaa ttacttattc tggcttacag ggtttttgtt accatttgac cagccttgga
   105961 taaattatgc gcttggcctg ttgcttttat tttttgttgt acaatggctg acaggaaaat
   106021 atcagattaa aattcaaaac cctttgttgt ggctgcctct ctgttatttt ctgctgcatt
   106081 gtataggcat gctttacaca caatcgcaat ataccggttg gattgaatta tcggcaaaaa
   106141 gttctttcct tatttttcca ctactcttct cgtccaatgc catttcgcgt gatataatta
   106201 agacctttaa gttatttttt atagcaggaa gcggaataat atgcctgtat aatctgctgc
   106261 tgtcattcaa tcaatatatt cagcatggca acatgagtgc attttattat gtcgcgtttt
   106321 ccaaaggtat gcatgtgcag tatctgacaa tttatttaaa tcttgcaata ctatttatct
   106381 tagaaatact ttttgacgct tcctctcaaa caaaaccaat caaaaaagct tattgggtat
   106441 tattggtctt gttcttttta gtcaatataa ccttgcttgc cagcagactt gccaccgtag
   106501 tatcttatgc aacaattttg ttttataccg ttgtgagaat tatttcatca ccaaattacc
   106561 tacgatactc cattcttgca gcattgtttg cagcttctgt gctgattata gattattttg
   106621 catttaagaa gattaaccgc ttcgagcagg tcgaaatatt tttaaaagaa aacacaaact
   106681 atgttgactt ttcaaaaact gaatacaaca gcaccacgtt aagaattcca ctttggatta
   106741 atgcttatga agtcattcag cgcaatccat tatttggtgt aggaacgggc gatgttaagt
   106801 caagtttaga ttctatgtat ctgaaaaata aatttactta tgcccatcag aatcacttca
   106861 acccgcataa tcagtatcta caaacaggtg ttgcttttgg gctgacagga atttctttat
   106921 taatgttaat gatgcttatt ccgctctatt atgcaatcag gaatagacac tatattttca
   106981 ttgcatttca atgtattttt ttattgaata tgctaacaga gtcaatctta gaaaggcagg
   107041 caggcattat tttattctgt cttttctatg ctattttcag ctctgaatat gttgccgcag
   107101 cattaaagac agtacccgaa caaaacaata cgtaatttta caacctaatt tcaatttatg
   107161 aaagtaccat ttgcaccacc tcgcatggac caacgtgtca ttgatgaagt tgcagacaca
   107221 cttctttcgg gctggataac aacagggcct aagactaaaa agtttgaaaa actacttaca
   107281 gcatactgca atgtgcctaa tgtgcttgcc ctgaattctg ctactgcagg tcttgaaata
   107341 atgcttcgct ggtttggtgt aaaagaaggt gatgaagtaa ttttaccggc atacacctac
   107401 tctgctactg ccaacgtagt gattcattgt ggtgcaaaac ctgtgtttgt tgatgtgggt
   107461 gatgatttta atattctgcc ctctgaaatt gaaaacgcca tcaacaaaaa tacaaaggtg
   107521 attatgcctg ttgattttgg tgggctgccc tgcgattttc aaaaagtaaa taatatattt
   107581 caatcagaaa atatcaaaaa taaatttgtg cctgcaaccg atgtgcagca gcaattagga
   107641 cgcattttag ttttgtctga tgctgcacat tcttttggcg ctactgtcaa caatattaaa
   107701 actggagcgc tgaccgatgt ttctgttttt tcttttcatg ctgtaaaaaa cctaactaca
   107761 ggtgaaggtg gtgccattgc attaaacctg ccaaagcctt tcgataatca aagcatatat
   107821 gactatctgt gcatttacac cctgcatgga cagaataaag atgcactggc aaaaatgcaa
   107881 aaaggcaatt ggcgttacga tattattgaa gccggattta aatgtaatat gacagatatt
   107941 atggcgtcca ttggtctagt agaacttgaa cgctatgcag aaacattaga acgcagaaaa
   108001 caaattgttg caaactacaa tgaggcattt gcaaaatatc catgggcgca attgcccgaa
   108061 tttaaatcaa actggcgtga aagctcctat cacctctatc ctatcagaat aaaaaactgt
   108121 acagaaaaac aacgtgatgc cattatgcaa aaaatgtttg atgctgatat agcagtgaat
   108181 gtacatttta ttcctgttcc tatgttttcg ttttatacca aattaggtta tagtcttcaa
   108241 cattatccca aagcaaagga caactatgaa agagaaattt ctttaccgct tttttacaac
   108301 ctcaccgaag aacaacaaca atatgttatt gacaatttga taaaggctgt gaatgagacg
   108361 ctagtacatc ggcctgcaat taactcctga atgattttgt gcttttcttc ttcattaaaa
   108421 tattctgtgc tttttgaatg atttttttgc ccatgtttta cacttttttt gtgtaactct
   108481 attttagggc aagacattgc aggttttcaa aagattcatc tcctacttta acctcccgat
   108541 agctatcggg attatcagac ttaattctct tttgtgccat cgaatataaa tttgatacaa
   108601 tgttaggtct tcttgtttta attccggtgt tataccctaa ctgccgcatc catctaagaa
   108661 gctgtccgtg ctcttcaact tcacctgtgt atttttccca aatttccact ttggtgcatt
   108721 gggctgaaat taactcctga atgatcttgt gtttttcttc tacggtaaaa tacttacccg
   108781 ctttttgaat gattttttta tccatgtttt acacttttta gtgtaaacct atttcaggac
   108841 aagacatgtg tgtgcttttg tgtgttggct actttgcatt tagttcttcc cttagtttat
   108901 tcattgagtt ttcaaacagt ttattgcttt tcaatttaca ttcccaaata gttaatactg
   108961 tccaacctga tttttttaat tctgccttta cttttttgtc tcgttcaata ttgttctgaa
   109021 tttttgtttt ccaaaatttt gcatttgaag ttggcaactt tgccttgttg caattatgtc
   109081 catgccaaaa acatccatga acaaaaacta tcgtttgata cttgggtaaa actatatctg
   109141 gtgtccctgc aagagttttt acatttttcc gaaacttaaa tccttcttta aacaaaaact
   109201 ttctgactga tatttccggc ttagtttcct tcccggaaat tgaagccata atttcacttc
   109261 gtttccgttt agaaaaaatg tcagccattt aatgcacgaa ttgattcaat aattgttaca
   109321 atatcgttgt aactatttgg tttatcactc agccactttt ctaaaaatgt tttctcactt
   109381 gcttcaatat ttctcttatc accagtcatt gcctccgcaa ctaaaagaaa tttctctttg
   109441 aaatcttgtg tagcgttgat tctttgaatg tattcaattt tacccatcgc agtcaaaata
   109501 gtttcagcta aaacttgatt ccaaataatt tcttctggaa ctgataatgg taagtaggaa
   109561 acatttcgca aataaaattt cagatacttt tctcgcaaca ctttttcttg gtctgctctt
   109621 gcccctgcat ttggatttcc gtcaggataa aacttaacat tgactcctgt ttgattttgg
   109681 atttcctgtt ggtatcgttc attggtttgt tctgctgcta caatagaatc aggattaaag
   109741 tgagcatgtc tttggtctcc gtcaaaaact ataaacttat tccgttcgtt tgcatgactg
   109801 taaacactaa tgaaatgttc tttaataacg ctttcacctc ccgtgtaaaa agtaacattg
   109861 aaaagtgaag cagttgcttg tcctaattct cttaatacac catcaataat ttgctttgaa
   109921 agcaaatctt caacaatgat tgttttcctt gccggatttg tttgctccaa atgataaaac
   109981 gcttcatcag gtgtgatttc atttttaaca ttgaattttc cagaaggtaa tagtgagaaa
   110041 acttttattg cgtttggcgg aagttcctca ataatacttg gtgagtgggt tgagattaca
   110101 acttgtaatc tttttcgtct tgtttcttca agcaaaaatt gcttcaatcg tttttgcgct
   110161 cccggatgca atgaaacttc cggctcatca agtaaaatga gagaatattc ttttgctcct
   110221 aaaacttcaa gcaccaactt tacaactgcc atttctccgc ttcctgcaaa cgcttcggaa
   110281 tatgagtaac tggcggtttc aaaaattacg gagtagcccc attcttcaaa aagtttatgc
   110341 tttacaaact ttcccgaaac atatttttta ccaagtatgg tagaaatatg ctgaagttca
   110401 attgctgtga aagttttgag ttgctcgttt ttgttttcac cgtagcgatg aattataaca
   110461 tttgttccgt caataattct tcgaagaaaa actgaatgct tccgtaagta gtcctgtttg
   110521 ttatttatcg ttttagactt tcttggtttc ttgaaataaa agtatttgtc aaatgcactt
   110581 aactctgaac ggaaatcaat atacactaca tctttttcaa tcggttgatt tctttttccc
   110641 tctgctatct tctgcattcc gtagctgata ttcggtcttg cagtttccca atagtccgga
   110701 ttgtttcgtt tgaaaattct ttgtttcaaa acttcaagcg gtctgccacg attgtctttg
   110761 taaacataaa agaagctatg cctatcgcct ccttccgaaa cttctacaat cgggtcaaca
   110821 gcagttgaaa accaaaattc aaacggagta ttttttttcg gtgctccata aagtgcatga
   110881 agtgttgaac tttttcccga accgttttgc cctacaaaaa ctgtcagagg aaaatcaaaa
   110941 attattcgtg tgtttttttc aaggtttcgg aaatatggaa actgaatata ctcaatgaaa
   111001 tttctgaaag catttcttgc tttcattctt gaaatttcag ctatgagagt tggtatattc
   111061 acaaatgcta tggtttttca gagtcgttaa gtcgtttgaa taaatgagat aacaaactaa
   111121 ggaagtccaa acaatctttt tctgaaaaca aacctgtttt acttaactca aggtgttcct
   111181 cgtgttgcaa agggtttctg ccgcctgcaa caataccttc ggatagatag tgttgtcctt
   111241 cttcaatatt ggttattgta tcttttgtaa aatctgttcc atcagttttc ttatactttt
   111301 tcgttaccga aagtttacga ccagaaaaaa cttctcccat catacttctt tcgctgccat
   111361 ttgtgctacc tgacttcttt cttacttctg aggtatatcg ttttaatgct tcaataaaag
   111421 ccgtgtaata gtcagcactc tgataatatt tttcagacac cactttaatt tcattttcta
   111481 aatttctcca atggagattt gcatattcag gggctatatc atgcaataat cttgtcacca
   111541 cctgttgtgt gtcttctgaa atttcaggtg tgtcatccaa taggtttaca attgtttcaa
   111601 cttgcccttg cacattttga ggaagttttt ctaaccaatc ttttacattc aagtttacct
   111661 tttcacttat cttctttttc tttttctcct tacgaaattc acgccactgc ctttctatta
   111721 ttgaaaggca tgatgcaata tatttcctga gtgcggatgt cttggcattt tcccagtcaa
   111781 tggattgacg gtttgttgag attacatctt cttcccaatt gtcaataaaa tctacattca
   111841 accatcctgt ggcataagaa taaaagtggc ttgattctga tggaccaaaa aattcaggtg
   111901 tgttaaccat tctgccgtta gcaaaaagtg taacgcctct gagagcagct ttgagaggtt
   111961 tttctgtggt aattattgtt ccatgaattt gaactttgtc ggaataatcc ttctcattta
   112021 ctttgataat ttcttgtatg tcccactcaa attcaggagt aatgttatca tacttcagtt
   112081 tgttatcaat ctttatcggc ttgctatcat tcagcaataa gtaaacaata aacttgtctt
   112141 gaaagttgaa aagttttgca atgctttctg catattcttc aagaggaaat ccggtttttc
   112201 ttttcagatt ggttaatgtg attcttgttc ctttgaaaat ttccttttct gataaatggt
   112261 caaatctggg ttcataatct ttccctttcc aagcaagtat ttcattccag tcaagattga
   112321 aacttactcc cgattttcct tggcaagttg taatgtttat agtgtcgcca attccaaata
   112381 gagctaactt tcctaatcct ttttttcctg ttggaattct atcgcaactt gatttttgac
   112441 cttcctctcg tctgtttctt cctattctta aaaagtaatc attcacttcc gcaaacgaca
   112501 ttcccgttcc gttatcctca acaattactt ttttctcccc ttcgtcatac aaacgcacat
   112561 gaacctctgt agcacacgca tcataagcgt ttgcaattaa ttccgctaat gccggtggca
   112621 tgttggaata cattttaaca ccaagatgtt ttatggtgtt agcttcaaag gtcatgtaaa
   112681 gtttatcatt gctcatttcg gattacaatt tatgatgctt gcagcaacac gcattgataa
   112741 ttgcggtgga accgcattgc caatcaatct tgctgttgat gcaattgaag tagaaagaaa
   112801 tttgtaatca gtgtcaaaag tttgcagcgt tgcaccttct cttaatgaaa tggctctgtt
   112861 ctgttctgga tgagcaaatc ttccgttggt tatactataa aactttgttg tgattgttgg
   112921 tccaggtttg tcccaaaaca ttctaccata gatgtcttga aatgagttgt ctttgccaac
   112981 ataagccgga atttgcaagt cagtatcttt ccacgaagtt cttgttcctc cgtcatgtgg
   113041 agttaagagc aatcgcttta aattattgtc tgatagtccg gctgaactgt gaaggaactc
   113101 cgtcttatct ttttgccccg ctgtaattgg tttgaaaacc tttttattgc caatgaaatt
   113161 ccgaactgtc ggcagctttg tttttgtgtc gggaagtggc aaagatattt tgtcggtaac
   113221 tcgtgatgca atcaacagga atcttcttct tgtttgtggc actccgtaat ggctggcatt
   113281 aattactttg tatttgtagt gatagttttc tttttctaga aagtcaagaa atgatttcaa
   113341 agggctttca tctaaacgat ttaaaatccc tggaacattt tcaaccacta tgtaaccagg
   113401 tttgaagtaa tcaacaaatc tctgaaagtc cttcaataag ttttttgttt catgtgcctt
   113461 agttttatcg gtttgaatct tgctccagta ttggcatggg ctgcacccaa tgaaaatcat
   113521 ttttttgtca ttgcgtttta ctttcagctc tttttcaatt cttgaaaaag ctaagtcctg
   113581 aatgtcagca tgtataaatt ttgaatcggg attattagtt gtatacgttt ctttacactc
   113641 taaatcaata tcaattccgc caacaacgtt tactcctgcc ttccgaaaac cgcttgtcat
   113701 gccgcctccg gaacagaaga aatccacagc tttaaatttt ctttttgtct ttgatttttt
   113761 acctttcata aacggctgcg aatttatgat tttcactctt aaattcatac gaaatcgcaa
   113821 taaagcgttg gcaaaaagtg gctctttttg ttttgcctct gtggtgggtc gtgtgttgcg
   113881 gcaaaacaaa atgtgccact tgtgcggttg ccatagaaat catttttttt tgtgcgttgg
   113941 cggtgtttta atttttttga agcggttgcc tgttggttag aagtgctgtc ataattatta
   114001 cttgtcatct taaatgtttg ttcaaagtcc gtccgtttaa tgtccgcatg gtcgcaaata
   114061 gagttaccga taacgaagaa gggattaacg aagtttaaaa aagaaggaga tagaaagcac
   114121 acactctccg caccgatacg ctttattacc tgcactcact ttcaagtggc tgtaatgccc
   114181 ttctttttta aatttcgttt aatctattgt tatgcgttcg tgctacgatc tacacgaaga
   114241 gtcagatcta tattcactta ttataaatta caatggtgtt attaaaactt ttaatcttcc
   114301 atcaccgtcc cattctgctt gagttccgtt tagtcctgtt cttctcattg ttgctccatt
   114361 tcttgatggc catgctttga tttggtcacc tggtttaatt cctcttacaa catactcatt
   114421 gcttacaaga ttttgtgcaa gaccatttat gcaacgtcct ccatttgtaa cttctggtct
   114481 tataattttc aaatcttcaa aatgtttatt tacagcttga ccttctaagc agaaattaac
   114541 aaattctttc tcaccttcat ctgttttaga cccaacatcc atgatgacaa tcttgtaatc
   114601 acccgcttga gtaaattcat aagcacccca agcacttgca gattctacaa taaaattaga
   114661 ggaatggatc gtggttgtat aggcaggtgt tatcattgca gagacaggtg ttttggttat
   114721 agagtcaggg gctgttattt taaagccagg tgtcactgct gtggttgtgg tcgtagtgga
   114781 aactgatttt tttccggtgc cacaggatga taagaaaaat actattgaga taagagtaat
   114841 gggtattaat tttttcattg catgtaaatt tattttgttt tgcttactct ttgacggatt
   114901 ttcagcttac tccatattat atattggtgt caatgtttat ttcttccgat cactttcaaa
   114961 attagcactc cccccaagca tgacgcataa catttatatt atcaacactc acaattcaca
   115021 aatttattca ttttgaaaca aatataaaaa ttattcgcaa ctcaaggtgc ggattatcgc
   115081 aactgtcaaa agcgtagttt gccttaggaa gtttgaatgt ttggcaagtt gcaaggttta
   115141 gaaggttaca atgttttgaa tgtttgaagg ttttgaatgt tacaatgttt tgaaggtttg
   115201 agggttttta aaatgttgtt gccttgaact tgcaatgtct aaattgaatt ctctttcaaa
   115261 attttcaaac ttttcagccg tggctaattt tcaaacttgt cagccgttgc tgattatcta
   115321 attatcaaat tttcaaatta cctcagtatt atttactcca tcactcctat cagccgtggc
   115381 taattttcaa attatctaat tatcaaattt tcaaattacc tcagtattat ttacttcatc
   115441 acttctatca gttgtggctc agccgtggct gattatcaaa tcatctcatt ttcaaattat
   115501 caaactctct ttatcattca taaaaaaaaa caccgaaatc taaaaaactg attccggtgt
   115561 ttttctaaac gggattattc ttttaccgtt ttataatctt tgaattgtaa aatttattgt
   115621 ctgcttttat ttgcagatag tacatgcctt gtgagatatc attcagcggc attttataaa
   115681 catctgaaat gatattagct gctgaataca ccagcttacc atccattgca aataatttca
   115741 cttcaacatt tttaaaatct ttggtaatta tggttaactc atcctgagca ggattgggat
   115801 aaacagttac actattgata gaactaaaat ctttagccga agttaccatt gttgatggtg
   115861 gaaactgaat attatcgagc atagccaaat cctgtccgcc cgtgcaacac acttcatcct
   115921 taatatatgc ccacgtgagt gtatgttgtc ctaaagttat cggatatgat ttatatgccc
   115981 agccatacac accggaatag gaatcaatca atgtaccatc tatgtagaaa ctgagaaaat
   116041 cccatcccat ttcacttgat atgagcatat aaaacgaaat agaatcgttg ttataaacat
   116101 ttactgttat tgccagatca gaggtatcgc catcagcaat tgttgcagaa cgtgcagaat
   116161 acgccccttg aaaagttccg ctgttagtaa tagtccatgg atgacttcca ttattaagcc
   116221 agggatatgt agtgaaattt ccgtattcga aattctctaa aatctcccct gcaaccatgt
   116281 taaatgtctt gttgtcggaa tatgggttgg catcaatatt aaattgcata tcgaaggttg
   116341 aaccaatctg aatgtttgat gcaagtgaga cattaaaggt agcatcaaca tcagataatg
   116401 cctgcacaac acctgtattg tacgaagtat tgttgatagt aagatatgga ctgcttgttg
   116461 ccagcgttgc tgttgaagct gtagttgcac tatgtccttt gttatttgtc tttattgtaa
   116521 catcggcagt ttcacctggt tcaagaatac cgttgttgtt tcctgtaggg tcagacactg
   116581 taatggaata tacttgcagc ataggtgctt tcaacaaatg tgttatggat gtactccaca
   116641 ctgtacctgc tgaatcggtt aatacaatat caaatactgc aggtgtgtta tcaggaacaa
   116701 aagctgcaac agtgtaatta aatgcattgg taattccagc agcgtttcca ctctgaatat
   116761 tcactttgtt taaactgctg ctgtttattg taacatacgg acaagtagta gttaccgtaa
   116821 gtccaacccc cacagcatcg ccctgtccaa agttttcaag cattaagtca acatctataa
   116881 tctcattata atctgcaagt tgatttgcat ttcctcctgc atcattaata acattgcttg
   116941 tcagcgaaac gtaaggtcct gtagttgcta caacaggaat tgccaccatg tatggaatgc
   117001 cgttaaatgc agtaattgtt aaatacagag tatcgagtga agttatcgtg ttaaaattta
   117061 aaattacatt ccctcctatc accaatcctg ttgcaagtat ttgtccattg ctgctcaaac
   117121 atgcaagtgc actgtctgta ttacaggcaa tatttaattg tgaagcaccg ggaaacaata
   117181 cagggctgtg actagcagta attgcataag gtgttgctgt acgaacattt aatgaagggt
   117241 cgccaaaaca atgccatgtg tctgtcattt cgaaacctgc tgcaccatac tgatcattca
   117301 tgtgcatgca gccattcata ctcagtccgc caaaagttct tttaatgttt gaagaatttg
   117361 tgccaaccaa taagtcaacc atttcgtcct gtgcatccat tggcggattc catgattggt
   117421 ttatgctcga cataaatgtg gctactgcac ctgaaggctg tccattatat tgtgctctca
   117481 aaaatgattc tgcaagacaa gtagcatttg caaactctcc attgacacaa gctacactcc
   117541 agataaaagg caacatgcct gtgtttgtta atgctgttgc attggcatta gaaaaacctg
   117601 ttgtgcctaa tgaagcagaa cttccatgac cagtataagt gattatagaa gcaccattat
   117661 ttatttctga tgataacatt gcagcagtag gattacccga catatcgttt ccaccttgtg
   117721 agccatcata caactcagca acatcagtat aagtgaatgc taattctttt ccgcgtatca
   117781 cctgctgatg ctgaaagtcc atttcattgt catcacccgg tccttggtca gagccaatgc
   117841 atattccttt tgaataccag tttacacctg gcgttggcaa catttcataa tcaacagtac
   117901 gctgaacttg tgtagtaaca tctgccactg tctgtgcact gaatcgtcca acaaatactt
   117961 ctgcataaga atcattcccc acaagataac catacgatgg gtcagatgca ccacccgaca
   118021 aaacaggagt aggaatttgt tgtgcatcgc ctaccaacaa aacatatttt aaattatgtg
   118081 tgctgtaata attctgaatg aatgttttta tctgttgtgc tgtgctgcct gctgtggcta
   118141 catcaaccat tgaaacctgc aagcctcttt ttatcttcca gtcaataaag ggctgcatag
   118201 ctgccataaa agatgcatca ctgataatga gcatttcacc atcttcattc accggtaaat
   118261 aattaataaa tgaacctgca ttgcgaaaaa cgttctgata gatatttgca aaatcacgat
   118321 caactttctg gagtgatgtt gtgcgagata atgtattaac agaagtgccg gctttacttt
   118381 gcagggtaac tttaaatttt gaaattactt ttaaagtttg tgttgctgca ttatagctga
   118441 atggatttac taccactgtc tgcccacgaa aatcacgaag tataaacggg ctacgcaaaa
   118501 agccactgtt agcaggaaaa aaaacattct gactgtaaat atctccaaag cgataaggca
   118561 catcagatgg cttaatatct ctgtaaagat ttcctttaga tggtgctatc tgaatattct
   118621 gaaaagtaag ttcctcaatt gactcaatat tcaggctcat atctccgtca tcaggaataa
   118681 tcacagataa agcaaacttt ggcaaatccg gtgttccttc ttccattctt ggagtcattt
   118741 cggggcttga aatggtatag tactctcctg ctaaagattt tactctgtgc ttttcaaatc
   118801 ctgaaacaga aatttccatt acagtattat caggtgtacc acttactata acagcagatt
   118861 ttgaaggatt gtcagaggta atcttaaccc attctgcatt tccctgtaaa gcaaacaatg
   118921 ctataaagaa agtggtaacg attgtctttt tttgcatgac taaatttgtt tactcaaaat
   118981 taagtctttt tttgaatact atccgtgatt ttaacaaaat aatttaccac agccccttgt
   119041 taactttgtt gaaatatttt ttcaggttag tgaataattg tttctgaatc tgaagcaaaa
   119101 aactaatttt gcacttataa ttcaatctta tttatacatg gctgtatatc gttttaaagt
   119161 atcgttcgaa gattttgaag atatttatcg tgaaatagaa gttacctcag agcaaacctt
   119221 tgccgatttt tataaagcta ttctgtcttc aataaaattt gaagatcgcg gaacagtttc
   119281 attttttatg agcaatgaca gttggaaaaa aggtgttgaa atttccaaca acgacaaaac
   119341 cgatcgtgat ggaaaacctg ttgctaaaac cgacaaagca tttctaaaaa gttatattgc
   119401 tgatccgcat cagaaaattt attttttaat tgactgcgaa agcgtttggg catttcatat
   119461 tgaattgata aagattatgg ctgctgctga tgtaaaactt acttatccgc acataagaaa
   119521 agtttcgggt gatgcaccaa gccagtttaa ggttaaagta gcaccggtac ctgacgaagg
   119581 tgacgaagta ttggattcag ataccaacga agatgataat ttcagttcaa ctgctaatga
   119641 aattgcagga gaaaatgatt atgatgaaga tgacacagca gctcttgaag gtgaagaagg
   119701 tgaagaaagt tctgacgagg atagtgagga gtttaatgat gttgaaatgg gcagtgatga
   119761 aaattacgaa gaaaattaaa agtgtatttt atattcttaa aagcacatct gttgatgtgc
   119821 ttttttaatt tttataaaaa tgaaagcagg cgataaagtt agctttaaag acgaaaaact
   119881 gaatggtgtc attcttgaaa ttttacagaa tggaaaagta ttggttgagt tggaagatgg
   119941 ttttcctatg gaagcattgc aaaatcaatt ggtagttact gttcctttta ttaaacaaca
   120001 aacacaaaat gatgctaagg aagatatatc tgaacaaaat acttcaacag aaaatatccc
   120061 attcgacctg aacaataata ttattgactt ttatgccgta ccatcaaagg ccgacagggt
   120121 acttacaggt agcgttgatc attttttaac aaatggcaca cccaatctac tattgctgat
   120181 tttcggaaaa cgaagcaaga accaacacac aattttatac aagggtgaat taaaacccaa
   120241 tagttatgta tgcctttcta tatttaaacg agacgcatta attaacttag attcgtttta
   120301 catacaaata ttaattgctg ccgatggcga atataaaaga ccttttacta aagatattgc
   120361 tgttgaattg cctgagctga atcaaactaa aaatacagaa acaggaattc tttcttcttc
   120421 taaaaaagta agccttgtca atctgaatgt tgcagatgaa attcagataa aagacttatt
   120481 agaaaaattt gacagcagtc agccagcaaa aacttcagac atcaaaaaga ataaaaaaca
   120541 acaaccaata agcgattctg acatcagcaa agaaattgat ttgcatataa ataaacttac
   120601 tgctgatttt cagaatatga ccaacagcga aatgcttgcg ttacaaataa atactttcag
   120661 aaaagaaatg gacaatgcaa ttataaaaga ttacaagcag ctaattttca ttcatggcat
   120721 tggtgatggt atattgcgaa atgcaataat gaatgaaact aaaaattatt cagacataaa
   120781 agtgtttatg gctaatcagg caagatatgg aacaggtgct ttggaaatac gctttttata
   120841 aactattctg cgaatgatgt gcacgaaggt tgttcgtcca ctgattaaaa aactgttctt
   120901 gttctgcgtt ctttttgctg atgtgtttta ccttccaggt cgaaaaatat ttttttgatg
   120961 caggcatcat ttcaatagtc tttattctgt tacctcttga aatcaaaaaa cctgttttgt
   121021 ggttttcctt aggattaatc tgcatgtcat aatttttatc tgccttttgc ccatctacgc
   121081 caataatcaa atcacctata gcaagaccat tctcaaatgc cggtgaacct attggcaaac
   121141 taattacttc tggcattgct gcattatcat tggtcttaaa accaaaatat ctttctgcat
   121201 tctttttaga aggattctct tctagttcca atcctgcaaa caacatcact tcttcaagca
   121261 acgattgata gctgcaagcc ttgtaaacaa tgtcgttaaa gaaagtttct aaacttctac
   121321 ctgccttttc agaagcaatt cttaaataat catctgatgt atatccaata ttctttttac
   121381 caaacgcatt atacaattct ataaaaacat catctaatga attttttccg tcagaatatt
   121441 tacgaatcaa aaaatcaagc atcagcgaca ctaatgagcc ttcatcataa attgaagttt
   121501 ttcgtccggg aattcccggc acatatccat caagccaggt gtcaaaagat gattgtgcaa
   121561 ttgaataatt aaaccggcct tcattgctaa gatgtttgtt gatacggatg ttcaactcat
   121621 taaagtattc ttcttcgttg aaataaccac agcgtgccaa aaacaaatcg ccataatatg
   121681 ttgtagctcc ttcatagaca aatcctgttt ctgcataatt tgggctatca taattgtaag
   121741 gttgcatttc tacaggtctt atggttttaa tattccacac atgaaacaat tcatgtgatg
   121801 caactcccag taactcatta tacaattctc tgtcattaag ttttgccgaa gggccaatca
   121861 ctaaaacagt agatgccaaa tgttcaacac catgataaaa tgtatgtggc actgcctgaa
   121921 ttaaaaagtg ataatctttt acaggaattg atttcatcac attcaattgt atctgcgtaa
   121981 aagatttaaa atcttcaatc aacttttcta catcgggttt gaaatttccc tgaatccaaa
   122041 tatgaaaact gcaatcagac accgtataac catagtgcat caaatcatca gaagctataa
   122101 acgggctgtc aacaagttca tcaaaattct tagcagaaaa aacatttttt tctgtttgtg
   122161 tgagtgatgt tgctactctg ctgaagttac taactatctc aactgtgcag gcattgcccc
   122221 tgttggcatc agtataaaaa caacaatgta tgggatttat gtaaatctgt tcgtcattga
   122281 caaagcaggc accggcatca agctgattgc aatagtactg atagttgatt tttattgtat
   122341 catttttatt tgttttcagc agccatgagc tctttgaaac ttttttccat ttcacttcct
   122401 ttccattaat atcctgcact ctaaaattgc ggatattttt tgcaaagttt ccttcttcgt
   122461 acctgcccgg acgccagaca ggaagtttta ataaagtttc attaccgtca gtttgtatgg
   122521 ttagttcaac atcaacaaag tgtgaagctg cattggatac tgaaaaacga taatgcatca
   122581 tgctaataat ttaaaaggta attcatctgt tcagatactt tctgtgcatt gggtatcatc
   122641 gtaaattcta aagttgaatt taatggaatt gcaggcatat tctccgatcc catagcaaca
   122701 acaggtgcat ctaatgcagt gaaacattgc tgctgtatgc gacctgaaag tgcttcggca
   122761 aatgaatttt ttagaggctc ttctgtcagc accaaacaac ggtgatgctt tcttactgac
   122821 tcaaatattg tagtttcatc taatggatta agagtacgaa ggtcaataat ttcaattctt
   122881 cctttaaact cttgtgctgc tgcctttgcc cagtaaactc ccatgccgta agtaataact
   122941 gtcagcgtgt tttcatcctg caaagcctct gcctgttgca ctattcttgc ttttccaaat
   123001 ggaataatat aatcttcatc aggttcaata gtgcgtgcat cttctgtgcc ttttatctta
   123061 ctccagtaaa gtcctttgtg ttcaaatatt actacaggat ttggatcata atatgctgct
   123121 ttcatcaatc ccttcaggtc agcaccggta gatggatatg ctaccttaac acctctgatg
   123181 tttagcacta ctgtttcaac acttgaagaa tgatatggcc caccacttcc ataagcacca
   123241 ataggaacac ggattacgca agataccggc catttgccat tacttaaata gcaggaacgg
   123301 cttacctctg taaaaagttg atttaaaccc ggccatatat aatctgcaaa ctgtacttca
   123361 acaatcggtt tacatccaac agcactcatg ccaactgtag aacctatgat aaatgcttcc
   123421 tgtatgggtg tattaaaaac cctgtcatca ccaaacgttt tagctaacgt tgcagcctct
   123481 ctgaaaacgc caccaagtct ggctccaaca tcttgcccgt agaaaagaca ttcaggatgt
   123541 ttttgaagca attctttaac agcaaacaat gcacagtcaa ccatcactgt tggctcctta
   123601 ccggcaggct gacgcacacc tttttcttct gtcactgttg taggtgcata atcatgctta
   123661 aacaaatctt cgggcaatgg gtcgtcagcg gccttggcct ttgcgaaatc gtcttctaca
   123721 agggttcttg tttctttctt tattgcatcc aactcatcag aagtaaaacc tgcttcttca
   123781 agttgttgga taaactttgg taatggatct tttttctttg cttcttccaa atcatgtctg
   123841 taccactctc tgcgaacacc tgaagtatga tgtccaagta atggcacttt agcatgaatt
   123901 aaaaaaggtc tgcgttcagt tcggataata tcaagaactt ctttgatggt attgtaagac
   123961 tgaaaaaagt cggtaccatc aatactgcgt gtttccaatc ctttaaatcc ttttgcatat
   124021 tcagcagcat tcatggtgcg aatctctgag gcatgtgctg aaatatccca ctcattgtcc
   124081 tgaacaaaat aaagtatagg aagcttttta agcactgcca tctgaaaggc ttcagaaact
   124141 tcaccttctg ttacacatgc atcgccaagt gaacaaactg tgacaggata ttcaccttcg
   124201 gcatattcta caagattttg gttttcgaga taatgcatcc ctaaagcaat tcctgtcgta
   124261 ggtatagcct gcattcctgt agcactgctt tgatgaggaa tctttggcat atcatcacgt
   124321 ctcaacgaag gatgtgaata gtatgtacgt ccacctgaaa acggatcatc acgtttagcc
   124381 ataagctgca acatcagttc atagggtgtc attcctattg aaagcaaaat actgtcgtca
   124441 cgataatatg ctgagagata gtcaatgctt ttaagttgaa gacctaaagc aatctgcact
   124501 gcctcatgtc cacgcgagca ggcatgaaca taacgagcag taaatgttgc atgctcctcg
   124561 tacaaatcgc tcatggcacg tgctgtgcac atcagcctga atgctttgag aacagtttct
   124621 gttgatatag gtgtagattc caattttttt tctgcaatca tttttctaat gttaaagaat
   124681 ggctttaacc tgctttggtt ttaaatttct caatggcaat gcgcgtaaaa tccgacataa
   124741 ctaatttgcc actcatcccg gctctttcca ttagcaatgc atcccatcct tctgttcctt
   124801 tccagaaaat ttgtttcaac attttcattg cttcagggtt actggcagct agttgttgtg
   124861 ctaatttatg tacagcttta tctaagtctt caatgttatc gtgcagagaa gcaaacatgc
   124921 ctttctcttt tgcccactgt gcgttttgcc attctgtagc attaactgta agttgtgtaa
   124981 atgctcctgt accaattttt ctctctactg caggtcccac tacaaaagga cctatgccca
   125041 cagcaagttc gctaagttta acagaagccg aaacatgagc taaggtatag tctgcagaac
   125101 atgccaaacc tacaccaccg ccaacagctt ttccttgcac tctggcaata acaaactttg
   125161 gcgctttacg catagcatta attacacttg caaaaccaga aaagaattct ttgccttgtg
   125221 attcactgtt tatgcttatc agctcatcga atgatgctcc tgcacaaaag gctttctcgc
   125281 ctttactgcg aagtactata actttagctg attcattggc ccctgcttct tctattgtgg
   125341 cagcaagttt acgtaatagc actccgggaa gtgaattact ctgagggtga aaaaaagtaa
   125401 tttcagctac ctgatctttt ataattgatt caaccgaacc ttctgtcata tttgcaattg
   125461 ttatttctct aaaaaattaa aacttattct ttcgtgcaaa gataaaaaag cccatgaagt
   125521 ttaccagcta attgaaagtt aatataatag cttatgacaa tgctgttaag tacttctcaa
   125581 aatttagatg tattctaaat ttatacttct taaatttaat ctataattgc attaaatagc
   125641 aatacaaatt gacctaaaca catctactta ggtcataaaa ttttagccag cttatgcaat
   125701 ttaacattcc tgctcattta acaggattaa ccgataagga ggttgattcg tcacgcttaa
   125761 aatatggaat aaatcaaaaa aaaactgata caagaaattc gatttttaat tggattctgg
   125821 atttgttgaa agaaccaatg ctcatacttt tgcttgctgt atccgtaatt tacttaatta
   125881 taggagatca tggtgaagcc atctttatgt ttattgcaat tgttgctgtt tctgccattt
   125941 ctttttatca gga
//
LOCUS       DAOOOI010000002       112494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_22_length_112494_cov_48.449773, whole genome shotgun sequence.
ACCESSION   DAOOOI010000002 DAOOOI010000000
VERSION     DAOOOI010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 112494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 112494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..112494
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22_length_112494_cov_48.449773"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PKX59_00530"
     CDS             <1..190
                     /locus_tag="PKX59_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89377.1"
                     /translation="VKASVSFEELFESCTSKIHAVFIFLALLDLLQIHEVSINVGEGF
                     NNFVLQKPQDLFTHADRE"
     gene            174..1235
                     /locus_tag="PKX59_00535"
     CDS             174..1235
                     /locus_tag="PKX59_00535"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOZ89378.1"
                     /translation="MQIESKDVLRRLNPTKIIWPMLIGLSVAGWLFYDNYDGKVFSQI
                     DWTWHSTFWMILAGFSVFVRDLAYMIRIRVLTDNELTWYRSFIVIMLWEFSSALAPGI
                     IGGGFLFAIFILNREGINLGKSITTITFTSFLDGVFLAVMAPVVYLLVGKDALFSGVN
                     VGQTSLGTSIFYSFWVVYFIILAYKLFIAYALFVNPYLIKKILTGICSFPVLKRFRKG
                     AVETGDQLIIASNGLKDKNLKYWMLSLLATFASWTARYSIVNCLIHAFHGQTALSDLI
                     VYGKQVIMGIIILLSPTPGGSGIAELMFTDFLGEFIHKGLAPTLGILWRLISYYPYLI
                     VGALILPRWIRERIVKKIF"
     gene            1240..1452
                     /locus_tag="PKX59_00540"
     CDS             1240..1452
                     /locus_tag="PKX59_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89379.1"
                     /translation="MSNKRRIFLIVISVTIGMGLSVIIFLMKNKGKALSDMDYLWLGT
                     NLFFSLLIVGGIGLYFMKKKDNDLMK"
     gene            1488..2279
                     /locus_tag="PKX59_00545"
     CDS             1488..2279
                     /locus_tag="PKX59_00545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOZ89380.1"
                     /translation="MKKQTLVVALTIVFGLMTFAVQAHNEGKQVTYQAGNTNCNGYVA
                     YNAHIPKMPVVLVIPEWWGCNEYAKMRADMLASMGYFAFAVDMYGDGYIAPTPKEAGE
                     QASKFYTDNALAMERINAAIQKLKDFPMADVENMAIIGYCFGGSMALNAARANLPVKA
                     VVSFHGGLKGIANPAEIKPKILICHGGSDNFVSAEEVTAFKAEMDKVKASYRFIDYPG
                     ATHAFTNPEATAVGKKFDMPISYNAEADKKSWLDMKAFLAEHLKK"
     gene            complement(2281..3069)
                     /locus_tag="PKX59_00550"
     CDS             complement(2281..3069)
                     /locus_tag="PKX59_00550"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ89381.1"
                     /translation="MDYLDLNKKLWNERTNVHVDSAFYDNETFLKGKNSLNEIELNLL
                     GDVKGKTILHLQCHFGQDSISLARLGAQVTAVDFSEKAIETAIELNKKSGTGCKFVCS
                     DVYSLNTVLDQKFDIVFTSYGVIGWLPDMQKWADIISSFLKPGGVFVMAEFHPAVWMF
                     DNDFKYVQYSYFKSAPIIETDEGTYADKQAKITTTSVTWNHSLSEVMSSLLNSGLRIE
                     SFNEFDYSPYNCLNNMVEVSKNHFMIKGMENKLPLVYALSASKQ"
     gene            3155..4213
                     /locus_tag="PKX59_00555"
     CDS             3155..4213
                     /locus_tag="PKX59_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ89382.1"
                     /translation="MSHILLIEDNKEVRENTAEILELAGYTVTTASDGKKGIEAVQKN
                     KPALIICDIMMPVLDGYGVLHLLGKNPETSTIPFIFLTAKTERGDFRKGMEMGADDYI
                     TKPFDKLELLNAIESRLKKVEALKKEYSKDLEGLTNFIGEVGGKDHMQAFIENRKIRT
                     YKKKENIYKEGDYPRGMYYINHGKVKLYKSHAYGKELITNLLKEGDFFGYTSLIEEGD
                     YVESAQTLEDTEVVFIPKEDFLNLLFSNREIAKRFLLILNQKFNEQQDYLIELAYSSV
                     RKRVAEALILLSDKYKANRDERFSMSISREDLANIVGTATESLIRTLSDFKEEKLIEV
                     KDGKIVLINENKLKELKN"
     gene            complement(4249..4953)
                     /locus_tag="PKX59_00560"
     CDS             complement(4249..4953)
                     /locus_tag="PKX59_00560"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOZ89383.1"
                     /translation="MFKSLLTIALGLSLSFAGAQTSKQDTVLTPDPDKAGLFDCRINF
                     PDGRARAVGKIQNGKKEGLWRFYDTKGKIELLEEFEADVLNGLHLHFNENGYIEDEES
                     YRKGILHGLRNVYKYGVTLITSENYNNGKLDGMRFDYYENGKLKEKATYKNGIRDGIT
                     IWFKQDDKPTIQYTYKNGDLNGPAIFYANGEIQSEGNYVNNNEEGEWRVYEDGKLLKT
                     ILYKNGKVIKETAAKK"
     gene            5062..6135
                     /gene="prfA"
                     /locus_tag="PKX59_00565"
     CDS             5062..6135
                     /gene="prfA"
                     /locus_tag="PKX59_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOZ89384.1"
                     /translation="MLEKLEALKHRFEEIGQQLSDPSATTDMRRFQQLSRQYKELQEV
                     VETYERYKKVIDNYDQAYHVLNTEKDVDLKQMAKEEMETLEAEKNSLEDQIRLLLIPK
                     DPIDERNAVVEIRAGTGGDEASIFAGDLYRMYSRYFEKKGWKVDVIDENEGTAGGFKE
                     VIMEVSGDDVYGIMKYESGVHRVQRVPDTETMGRVHTSAATVVVLPEADDVDVVINPA
                     DLEFQTARSSGAGGQNVNKVETKVQLTHKPTGIMVTCQIERSQHGNRERALHMLKTKL
                     YEIELQKHESEIAARRKTMVSTGDRSAKIRTYNYPQSRVTDHRIGLSTYNLSSFMNGD
                     IQEFIDALQLAENAEKMKEASLS"
     gene            6139..6960
                     /gene="pyrF"
                     /locus_tag="PKX59_00570"
     CDS             6139..6960
                     /gene="pyrF"
                     /locus_tag="PKX59_00570"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOZ89385.1"
                     /translation="MNYQQLVGEIKRKQSFLCVGLDTDLKKIPSHIRDKADDPVFEFN
                     KAIIDATLDYAIAYKPNLAFYEASGVEGLKSLEKTIQYINGRAFTIADAKRGDIGNTA
                     EMYAHAFYNHYGFDSITLSPYMGADTIEPFLKHKDKWSIILALTSNPGAEDFEMLKVD
                     DDFLFEKVLKKFSKASAQQIMFVVGATRPQYFEIVRKHVPDNFLLVPGIGAQGGSLED
                     VCRYGLNSNAGLIVNSSRQIIYADSGIDFDKKAAEQAKQIQQQMKQMLVEFKITG"
     gene            6960..7694
                     /locus_tag="PKX59_00575"
     CDS             6960..7694
                     /locus_tag="PKX59_00575"
                     /inference="COORDINATES: protein motif:HMM:NF025175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HOZ89386.1"
                     /translation="MPFTLAHPAIVIPFYRLFKERLSLTGLIIGSMVPDVEYCVNTVT
                     RSVISHTEKGIWVFDLPVAIFLTFCYHGFVKQILTNNLPDWIRYRLSPYANKNWFHYF
                     RKNFLNYFLCLLAGIVLHLLWDSVSHGTGYFVRRTAFLQENVIGTLTVTRLIWHISTV
                     AGLYVVIRFFLSFPVNKMEEENNIPDKNFWPLVFLVASVLTIIHWLPFVRPQEPRFIL
                     VAFSGAMMVGVIVVCFIFRFFKKQLS"
     gene            7691..8965
                     /locus_tag="PKX59_00580"
     CDS             7691..8965
                     /locus_tag="PKX59_00580"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOZ89387.1"
                     /translation="MNEALLYHLWKYRLFKSLPLTTIDGKTIEVIKPGELNPDSGPDF
                     FNARLIIDGTEWAGNVEIDPTSADWYNHKHHINKAYSKLILHVVYEMNKPLDHGFPVL
                     EFKKFVDDGAVKTFHQLQESLLPIPCSKQVHHVNDVTLNTWLERMIIERLEQKTTVIH
                     DMLHANKFDWEETFYFFLARNFGFKTNALPFEMLARSIPMNALARHKNNLLQIEALLF
                     GQAGFLNDKHADEYIESLRREYRFLAGKFQLQAMNNSLWKFARMHPRNFPSVRIAQFA
                     ALIHNSTHLFSKILEEKDVKSLIQLFSITPSSYWDKHFHFGKESAESKKTFGRSSVDN
                     IIINTIAPFLFLYGKIKDNEKLTERALSLLEHIKPENNVIIRQWKEVGITVKHAYHTQ
                     ALIQLKNLYCSKKKCLSCALGVKILNNKKNDY"
     gene            8961..9179
                     /locus_tag="PKX59_00585"
     CDS             8961..9179
                     /locus_tag="PKX59_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOZ89388.1"
                     /translation="MDSIKNFFERQAFGICEYWGERLKIRSSVIRLFFIYLSFLTIGS
                     PVVVYMVLGFWMNLRKLIHRQRGGVWDL"
     gene            9225..9590
                     /locus_tag="PKX59_00590"
     CDS             9225..9590
                     /locus_tag="PKX59_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOZ89389.1"
                     /translation="MYIKPTCSTCRTAMQMAKECETEIEMIEYLIDTPSEKELLELTQ
                     QLGVKPFDLVRTKESLYKEKYEGKKISDKQWLKILHKNPILIERPIITDGTHAIIGRP
                     PTLIADFLNKKQKTKKKSK"
     gene            9663..11507
                     /locus_tag="PKX59_00595"
     CDS             9663..11507
                     /locus_tag="PKX59_00595"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOZ89390.1"
                     /translation="MLSAIQVNAQHDDLFIPRNIQKAYQNNTRTIEGAPGKKFWKNFA
                     EYFIQVKFNPKTLKIEGKQIITYYNNSPDTLKKLVFRIAPDFYKKGNERNRIIEPDDV
                     TDGVKFTNCVIDANKVDLNNPMLVKHEGTIMEIKVKGIDPRGGRATVEMDWSYTLNLN
                     SHERTGVIDIGSYFIAYFFPRITVYDDVDGWDEFPYLGQQETYFDNASFNVHITVPRN
                     FMVWATGDLQNPAEVLHESVLKKWTLAQQSNKPVFLIDSADIQNKTVTVNKKFNTFQF
                     KCTTPDFVFAVSNHYLWQARDMVVDSVTMRKALISTAFNPIHKDFFEVNDFTAKTIEV
                     MSFDFPGVAYPYSHLTVVDGRDQMEYPMMINDNPTTTRFDGITLTTHEVFHQIFPFMM
                     ATNQTKYAFMDEGWATIGEWYISPIIDSTIVDDYGMDRINSDLGKDWDTPMITNSTEL
                     TKSLFINSYPKPALTFLYLWEMMGDSLFKKSLQHYIITWMDKSPTPWDFFFSMNQSCG
                     MNLNWFWKAWYFEQGYPDLGIIGINRLNYSYTVTIKSIGNKPVPVHLTATFADGTKRK
                     FTRTVDIWKNRNITDVIIDTQQSITKLELGDVYDADVDKNNNVLVPKE"
     gene            11540..11611
                     /locus_tag="PKX59_00600"
     tRNA            11540..11611
                     /locus_tag="PKX59_00600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11573..11575,aa:Arg,seq:cct)
     gene            11585..11707
                     /locus_tag="PKX59_00605"
     CDS             11585..11707
                     /locus_tag="PKX59_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89391.1"
                     /translation="MAVRFCPGLRRKGSSLSDYEVGGDSARDYEEKAVPCPATK"
     gene            11747..12046
                     /locus_tag="PKX59_00610"
     CDS             11747..12046
                     /locus_tag="PKX59_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOZ89392.1"
                     /translation="MPYYVYVIRNKVTGRLYKGQTENLEKRLKAHSQSQTKTTSYFPK
                     DWELVYHEIFSSRKEAVEREKYFKTAAGRRYLSGRIANIKSTDTRPNVPFGTGGE"
     gene            12334..12747
                     /locus_tag="PKX59_00615"
     CDS             12334..12747
                     /locus_tag="PKX59_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOZ89393.1"
                     /translation="MHIHLFREQLKQKGLKVTPQRVAIYEAVAELHNHPTAENIIEYI
                     KTNHPNISVGTVYKVLDSLVENNLLRKVKNEKDIMRYDAVLEHHHHLYCAESDRIEDF
                     EDPQLDHFISEYFKKKKIKNFKVQDIKLQITGTFK"
     gene            12768..14951
                     /gene="katG"
                     /locus_tag="PKX59_00620"
     CDS             12768..14951
                     /gene="katG"
                     /locus_tag="PKX59_00620"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HOZ89394.1"
                     /translation="MDNTKSGKCPFHHGANTEASTGVMNWWPKALNLDILHQHDTKTN
                     PNGEKFNYAEEFKKLDLEALKSDLKKLMTESQEWWPADHGSYSGMFARTAWHLAGTYR
                     KADGRGGANTGNQRFAPLNSWPDNVNTDKGRRLLWPIKKKYGNKISWGDLIVLAGNMA
                     YEVAGLKTFGFGGGREDIWHPEKDIYWGSEKQWLAPTGSEGTRYSGERDLENPLAAVM
                     MGLIYVNPEGVDGKPDPLKTAQDIRVTFSRMAMSDEETVALTAGGHTVGKAHGNGKVE
                     VLGGVPEAAEIQHQGLGWFNPDGSGNKDKTMASGIEGAWTTDPTKWDNGFFDLLLNYD
                     WALTKSPAGAWQYEPTNMPEEKKPVDAFDPKVRRNPMMTDADMALKMDPEFRKISERF
                     YNDFNYFSETFARAWFKLTHRDMGPKSRYLGADVPKEDLIWQDPIPAVSYTLSDAEAD
                     EIKTKILNSGLSRTELINTAWDSARTYRGSDFRGGANGARIRLEPMKNWEGNEPKRLQ
                     KVLDKLTEIQKGLSKKVSIADLIVLGGSAAVEQAAKDAGMSVKVPFAAGRGDATLEQT
                     DVDSFSDLEPLHDGYRNYQQKNYAVSSEELLLDRTQLMGLTAPEMVVLVGGMRVLGTN
                     FGGSKHGVFTEREGVLTNDFFVNLTDMKYEWKPVSENLFNIVDRKTGATKYTATRVDL
                     VFGSNSILRSYSEVYAQDDNKEKFVKDFVKAWVKVMNADRYDLKK"
     gene            complement(15033..17630)
                     /locus_tag="PKX59_00625"
     CDS             complement(15033..17630)
                     /locus_tag="PKX59_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOZ89395.1"
                     /translation="MKRLLLFFAMIPFFGVAQITDNFSDGDFTANPAWSGDNSQFIVN
                     ASQQLQLNSTGAATSYLALTTSLPTLDNTSWQFYIKLNFAPSSSNYARIYLVSDQQNL
                     KGALNGYYVQFGETGSNDAIEIFKQTGTTSVSVARGTNGFIAAASTISVKITRDNAGL
                     WSIYADAAAGTNFTLQSSGTDATINSGSYFGVSCTYTSSNATGFFFDDFSIPFIADIT
                     PPTLTSAIPLSNTALDVKFSELVDLTTSQTTTNYAIQGLGTPATAVRDISDFSLVHLT
                     FSTPFTSGTAYTLSVSNVSDLNSNVIAAGSTIGFTWNAPVTAQPFDVVINEIYFEPIV
                     SAPLPNAEFVELYNRSNKILSLNGWTLSDGSTSVASIGAVTLQPSAYIIVCKNSDTTS
                     FIPYGNTTGTSSFPSLNNDVGDDLKLTDNNGTLIDRVIFSDDNYNDDSKKTGGFTIER
                     IDADFTCNSRFNWHASNDAQGGTPGKVNSVKGTYSDTTPPQAVDAYPLTTTLIRITFS
                     ENPDNAIASQTSSYIIDNGIGQPASVLLSDNHAFLTLNNALQTGIVYHVNLNSSIADC
                     PGNKITGELSLKIGIAETAVAGDVLINEILFNPKTGGNDFVELYNNSSKIIDLKNLKV
                     ATTDNNNLITTNYSVSDGGYLLFPKEYIALTSDVASLQSFYPNAATNNLLKASLPGFN
                     DDMGVCVITDFALNRIDQLNYNKRWHFPLIDDQNGVSLERIWFNKGTQDSTNWHSAAE
                     AVGYATPGYKNSQSLNEQLQENNFTITPEIFSPDNDGYNDVVTFNINMNEPGYMLNAK
                     IYNEAGQLVRNLVKSKLLAPEDSFMWDGITDKNDKASVGIYIVYAEAFNPEGKTKKYK
                     KAFVVASKL"
     gene            17748..18740
                     /locus_tag="PKX59_00630"
     CDS             17748..18740
                     /locus_tag="PKX59_00630"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOZ89396.1"
                     /translation="MKVAVVGATGLVGKTMMRILEERKFPVSEFIPVATANSAGKKVM
                     FSGKEYTVITCEEAVALKPDIALFSAGGSTSLEWAPRFAQVGTTVIDNSSAWRMNEKC
                     PLVVPEINADVLTKEDKIIANPNCSTIQMVMVLNPLHKKYKLKRIVVSTYQSVSGTGK
                     KAVDQLMNERKGISGAMAYPYNIDMNVLPHVDSFLENGYTKEEMKMVNETRKIMRSDK
                     IKVTATTVRVPVVGGHSEAVNAEFENQFELSDVRQLLSHTAGVTVIDDPAQLQYPMPT
                     YAEGKDDVFVGRIRRDESQPQTLNLWVVSDNLRKGAATNAVQIAEYLLKQFWQH"
     gene            19007..19246
                     /locus_tag="PKX59_00635"
     CDS             19007..19246
                     /locus_tag="PKX59_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOZ89397.1"
                     /translation="MNWQEYIMSDEEVLLGKPTIKGTRISVEHIVGLLAQGWTEKEIL
                     ENFPRLTKESLQAVFLYIQDCFSDGLLFTPKRKTA"
     gene            19256..19609
                     /locus_tag="PKX59_00640"
     CDS             19256..19609
                     /locus_tag="PKX59_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HOZ89398.1"
                     /translation="MKLLANENFPLKSIVYLQNGGFDITSIGAEHSSIKDFEVMTIAI
                     NEERTILTFDRDYGELIFKHNYKPPKGVIYLRLEKYSADEPGRLIEELVSSNQYIFDG
                     ALTVLDERGIRQRKY"
     gene            complement(19746..20423)
                     /locus_tag="PKX59_00645"
     CDS             complement(19746..20423)
                     /locus_tag="PKX59_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOZ89399.1"
                     /translation="MGIYLIVIVFMLVGWLVSAQLKRKFEQYSHETLGTGLSGQEIAE
                     KMLHDNGINDVKVISVEGQLTDHYNPLNKTVNLSQPVYYGRNAAAAAVAAHECGHAVQ
                     HAKAYTMLQVRSALVPVVSFASKWVQWVLLGGVLLINVFPSLLLAGIVLFAMTVLFSF
                     ITLPVEFDASRRALVWLSDSRMVTSSEHAKAKDALWWAAMTYVVAALGALATLMYYVL
                     IYFGRRN"
     gene            complement(20547..21638)
                     /locus_tag="PKX59_00650"
     CDS             complement(20547..21638)
                     /locus_tag="PKX59_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ89400.1"
                     /translation="MSYSQTKAMLAIAKASFRSITRSPSAVVFTLVFPLTFILVFGFL
                     SGTGLKVKLFLTPSSDHHNPIYEATKNIDVINWIEGDSAIASKKLQQGKIDGILVITK
                     NITEGKIDYTARLITSTAAPEGGALSKNILDNLISKINLYDAHVAHPLAQLKNEEIAG
                     REYKKIDFILPGQLGFALLSTGVFGTAFVFFSLRQTLVIKRFFATPVKRIYIVLGETL
                     SRMVFSLLGACFIIGVGRFAFGFTLINGISTFLTMMLICAFGLIVFMGFGFVVSGIAK
                     SESTIPPIANIITMPQFLLSGTFFPIDVFPKWLQPFCKALPLTYLNDALREVAFEGAG
                     LIDVISQLMVLLLWGVIIYIVAAKTFRWE"
     gene            21739..22290
                     /locus_tag="PKX59_00655"
     CDS             21739..22290
                     /locus_tag="PKX59_00655"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOZ89401.1"
                     /translation="MNHTFSLFQSMERDKIILSFKGDITQDLLASVYQIMESRMDQDK
                     TDSKRKKRFYHILVECLQNVYHHMEDFRNVHELDEISDGSAIFIIAKAKGEGYRIITG
                     NYIKKDESEDLKSKLEKINSMNTDELRTYYLETLSTTELSQKGGAGLGMIDIARKSGH
                     KIVYSFDGVSDTVNFFTLEVTIN"
     gene            22301..22684
                     /locus_tag="PKX59_00660"
     CDS             22301..22684
                     /locus_tag="PKX59_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOZ89402.1"
                     /translation="MDKLIIEPGIKTPHIVFDAANGSLEIKGKSIPENSIEFYKPVYD
                     WLDTYTTQPKDQTLLTVQLEYFNTSSSKCLLDLFRKLENIHSSGKGKVNVKWLYEEDD
                     EDIMEAGDDYQTIVKLPFSIEKISG"
     gene            complement(22685..24529)
                     /locus_tag="PKX59_00665"
     CDS             complement(22685..24529)
                     /locus_tag="PKX59_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOZ89403.1"
                     /translation="MKNILFLILIPFTMFAKKPEDERGIYVKDQHSYSNPAEIVVQHL
                     DLSLKADFSTKKLVGFVTLQVKNLTNADTLRLDTYNLKIESVKINDAPVTFILKPADE
                     ILGSELAIPITANTKEVTIYYETSPDAQALQWLNPEQTAGKKHPFLYTQSEAILARSW
                     IPLQDTPGIKFTWTATIHCPKELMALMSCPQNPKQIGNGTYSFKMPFAVPSYLMALSI
                     GDLAYKAYDNRSGIYAEPVMLAKAFHEFTDLPAMISSAEQLYGKYPWLQYDVLVLPPS
                     FPFGGMENPCLTFATPTIITGDRSLVSLIAHELAHSWSGNLVTNATWNDFWLNEGFTV
                     YFESRIMEKIYGKDYADMLNLLSQNELKHTVADMGVNNPDTKLFLDLKGRDPDDGMGD
                     IAYEKGRFFLMALEEAAGRDMWDAFLKKYFEEHRFQTMTTKEFLHYLEVNLLQENPLI
                     KQKVNVDEWVYGTGLPKSLPVIKSKYFDQVEKAAAAFIKQPTVMPDTAGWTTHHYLHF
                     LRTIAPEMTYEKAVALDNHFHFTQIQNSEIAFDWFMIAFACHYKPAYKALENFLNTVG
                     RRKFIAPLYRKAAADATLRPWIEQIYKSARPGYHAVAVKTIDEIFEGK"
     gene            complement(24537..24986)
                     /locus_tag="PKX59_00670"
     CDS             complement(24537..24986)
                     /locus_tag="PKX59_00670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ89404.1"
                     /translation="MNIFWKFKSFNDLTAHELHDFLQLRQQVFIVEQNCPYGDIDKTD
                     KLSFHLMAMANQQVVGYARIIPPGVLYDEVSVGRIVTHQQLRRTGLGKLVVEKSLDEC
                     KSLFPNSAIKIMAQSYLRKFYESFGFVVISEEFLEDNIPHIYMIKTK"
     gene            25126..26154
                     /locus_tag="PKX59_00675"
     CDS             25126..26154
                     /locus_tag="PKX59_00675"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HOZ89405.1"
                     /translation="MSLPQLIVTDSSRNGKLPFAPFKRTQEESAQQPKTEKVSNKVAK
                     QKLSLKTDSLKGNTTVVDTIKPIANDSIQLAIDSSVADMSRLFRFTDEENDELNNSDQ
                     QFISSSAYFKQQQLEVKSSSPQLFNKIVPDWFTIVLFFLIVGITTVKVFYQNIFRQLV
                     EAVYSFAVTNQVVRDENLLVQRASVLLNVIFYCSSGLFFYWISVRYNWNHPILNEGLF
                     RFFFFAFVTAVFFTVKMLLLKLIGTIFNLDKPVAVFIFSIFLTNNLLGVLLVLITSFM
                     AYLSGVGLINYIYLVLGILSLAFIFLLYRAFLISRTVQGYSLYYLILYFCTLEIVPIA
                     LIVKFASE"
     gene            26178..26933
                     /locus_tag="PKX59_00680"
     CDS             26178..26933
                     /locus_tag="PKX59_00680"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOZ89406.1"
                     /translation="MAGNSKVKTILVTQPKPETEKNPYFDLAKKFNVKIDFRPFIQIE
                     GIPAKDFRKDRVNVAEFQAVILTSRQAADHYFRMAAEMRMTNFDEVKFFCVSESTAFY
                     LQKYITYRKRKVFYGNNSINDLTDVLKKHKKEKFLLPCSEVMNPDIINTLKDGDLNFI
                     TAAMYRTVSSDLSDVNIETFDMIVFFSPSGVQSLLKNFPKYKQGNTRIATFGVTTSQT
                     AKDAKLKVEVEAPVPQAPSMTMAIEQYLKKANK"
     gene            complement(26938..28116)
                     /locus_tag="PKX59_00685"
     CDS             complement(26938..28116)
                     /locus_tag="PKX59_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89407.1"
                     /translation="MLSRKIEIFKTSDCVDKERLTKYVQGKMTDAEKYAVEKHLTDCE
                     LCSDAAEGLALLTSMKPLDETIKQVSDKYSSSAKQSPSINRWYYAAASVAILIATGWI
                     ISNYSFKHENTAEQINAPAVQQKINTETPQTSSDTKLNESTIQPPPPPAEERIQQASK
                     NIIASHNAMVAKASKEDKAFAATEPVQKNEAIAEPQSADVAAPIVTESASAGAAVVDA
                     VVPVTKNIEGLKVYDYSREYKREKNVAKEDNGIPARYESRKDMEKSKTLNNMQSRTIG
                     YQDFLAEALVDYNNKKYSSAINKLQLLLSDNHSDVNALFYTAMSYSESQQYDKALHFL
                     DRLDAQSNNTFNQESAWHRALLLLQKGEQDKAKELLQKIISSKGFYATQAQQKLSEIK
                     "
     gene            complement(28103..28699)
                     /locus_tag="PKX59_00690"
     CDS             complement(28103..28699)
                     /locus_tag="PKX59_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOZ89408.1"
                     /translation="MWLIRNKDKNALSDEELVLQYRQQGDKQAVGILFNRYSHLVFGV
                     CMKYLKDTDDSKDAVLQIFEKLYADLSKHDVQKFSYWIHRVAQNFCLMQLRSRQAAKN
                     RADNYSRDEIAASTEFEPENTDRVYEKETELLMLEEAIKTLNEEQRICIELFFLKEKC
                     YNEITDLTGFDFKQVKSFIQNGKRNLRIYMEKNNHVKS"
     gene            complement(28781..29059)
                     /locus_tag="PKX59_00695"
     CDS             complement(28781..29059)
                     /locus_tag="PKX59_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89409.1"
                     /translation="MELTIIAKEDLSDYHFVQHEVLSNEIDRARRKILLDGAMALGND
                     YKQKVRIVFETTEGTCAVETTVWAVTNDHVELKAGKDIPIHAIAEVVL"
     gene            complement(29125..30156)
                     /locus_tag="PKX59_00700"
     CDS             complement(29125..30156)
                     /locus_tag="PKX59_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89410.1"
                     /translation="MQRIFHILWCFCFTSSLQAQQIELKTIQLPVQVSIRALQTVNDS
                     VVWFAANRGVFGFTKDGGSNWHIDSIATDGKYSEFRSLAALNDSTVLLLSIDSPAYLL
                     RTNNYGTSWQHVYSNHSKGIFFDSMTFKDSLHGIAISDPMEGYFMIIKTTDGGNNWQE
                     LKVTSPAIKGEGCFAASNTNIQWLQNSVIFATGGSTSRLFISENDGAFLPQTTPMISD
                     STMTGIFTMYFENKLNGYIGGGNYLQSDTTTTLYQTNNGGKTWQPIKLPEGAFVSCVK
                     TIKTKNGNYLITTGHNGTYVIDSQHYIKEIKNADGLSLAFYTISIAPSQKKVWMAGKS
                     GKMAVLKFY"
     gene            30325..31545
                     /gene="dinB"
                     /locus_tag="PKX59_00705"
     CDS             30325..31545
                     /gene="dinB"
                     /locus_tag="PKX59_00705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOZ89411.1"
                     /translation="MSAQLNRVIAHMDLDCFFVSVSRLLHPELNNKPVIVGGGERGVV
                     AACSYETRKFGVHSAMPIKQAKRLCPEAIIVRGDYDAYSSHSDMVHEIICESVPLYER
                     SSIDEFYLDLTGMDRFFGCYKHATELRQRIIRETGLPISFGMSANKTVSKIATDEAKP
                     NNQMQVDCGEEKIFLAPLSVRKIPMVGEKTYTLLRSMGVEKVKTVQEMPVELMERVLG
                     ENGTTIWKKANGIDTTAVEPYHEQKSMSTEETFEQDTIDVQRLQHILIAMTEKLCFRL
                     RSEHKVTSCISVKIRYSNFDTHTMQCRIAYTSCDHIIIKQVKELFAKLFNRRMLIRLI
                     GIKFSHLVGGGHQINLFEDSEEIINLYQAMDRMRLRFGEDKIHRAAAMSFSLRGFNPF
                     NGLSNSPKEIKGGK"
     gene            complement(31702..32679)
                     /locus_tag="PKX59_00710"
     CDS             complement(31702..32679)
                     /locus_tag="PKX59_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003003667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOZ89412.1"
                     /translation="MLISVYVPYHAAIEAITPAYRLFKTANDILIASGKKPKFKVEYV
                     GLQKSIKANDGEYSIRIDRHINSVNDSDLIIIPALYGDVKHSLKKNAAAIPWLQKMHR
                     QGTEVASLCIGAFLLTASGLVDGKKVSTHWAYFNEFKEQFANTEVVDGAIITEERGIY
                     SSGGANSLWNLLFYLLEKYSDRHTAILASKYFAIDLDRNSQASFAIFNGQKNHHDTEI
                     LKAQQYIEKHTDAKLTIDQLADYTKMSRRTFERRFLKSTNHSVLEYIQRVKIETAKRK
                     FESSRKNITEVMFDVGYTDTKAFRDLFKKHTGITPLEYRNKYSKISLKG"
     gene            32821..33270
                     /locus_tag="PKX59_00715"
     CDS             32821..33270
                     /locus_tag="PKX59_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOZ89413.1"
                     /translation="MQTNNVNLHRVFASPTEKVFKAFSDADAYTAWLPPYGFVAKTHQ
                     YDFKVGGSYKISFINFTTGFSHSFGGEFVEIKLNDLIKITDRFDDPNLPGEMITTVHF
                     KKESCGTEINISQTGIPVAIPSEMCYLGWQDSLDKLKRLVEPNIPDA"
     gene            33337..33741
                     /locus_tag="PKX59_00720"
     CDS             33337..33741
                     /locus_tag="PKX59_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HOZ89414.1"
                     /translation="MSKQVFINLPVKDLKRSMSFYEAIGFTNNPQFTDHTAACMVWSD
                     TIFVMLLTHDKFKQFTSKLIIDAKQSVGVSNALFFESIDAMKSFAENAVKAGGREYAA
                     ASDYGFMQQHFIEDLDGHNWEPFYMDMSKFPG"
     gene            33762..34232
                     /locus_tag="PKX59_00725"
     CDS             33762..34232
                     /locus_tag="PKX59_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOZ89415.1"
                     /translation="MAKKKIAPCLWIEKDAKKAAKYYLSIFKEGKMLSYKAYKNSGTP
                     SGDFDIAYINFLGMDVQILAAGPLFKFNEAVSFVIICKDQKEIDYYWKALTKNGGSEG
                     PCGWCKDKYGLSWQVAPELYFKLEHHKNKAKQEYAFKALMKMKKIIIADLVEKK"
     gene            34263..34670
                     /locus_tag="PKX59_00730"
     CDS             34263..34670
                     /locus_tag="PKX59_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOZ89416.1"
                     /translation="MNKITVSAIINAPIKKVWDCYTNPNHIVNWNFADPSWHCPSATN
                     DMRIGGTYKARMEAKDGSFGFDFEAVYTQIESEKYFSYEFGGRVASVDFKNNDSQTEV
                     VVVFDAESENLVEMQRAGWQAILNNFKNYTEHN"
     gene            34674..35072
                     /locus_tag="PKX59_00735"
     CDS             34674..35072
                     /locus_tag="PKX59_00735"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOZ89417.1"
                     /translation="MQLCAYLHFNGDCRKAMTFYKKCLGGRLSFQMVSELPQSESLPT
                     YIKRNILTALLQTEYFQLAGTDLGNEAVYSPDKSFSLLLECRSKPELKRLYKQLSAGG
                     VKTQLLQKNFYGVIIGSFKDKFGINWVLQY"
     gene            35217..38195
                     /gene="dnaE"
                     /locus_tag="PKX59_00740"
     CDS             35217..38195
                     /gene="dnaE"
                     /locus_tag="PKX59_00740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOZ89418.1"
                     /translation="MHLCCHSYFSLKYGVLSPDDLLCIAAQKNIQSLALTDINNTSGI
                     LDFFRLAQRYNVRPVAGIDFRNGVEQKFIGIAKSNEGFHELNKFLSEHLISGEKFSDI
                     APAFQQAWIVYPMLSAPQQLHENEFVGIKPNELFQLNFSEWKSKLHKLVALAPATFRN
                     KTDFNSHRLLRAISYNTLLSKLPVSGQASADEIMYSPEELKCIYREYPQLIYNTEKLL
                     EPCCIDFEFGKNKNKKYFLGSAKRDHHKLMSLCYEGLSYRYPNTDDTIIDRFEKEIQM
                     IGELGFSSYFLINHDIVRYARSKNYFYVGRGSGANSMVAYLLRITDVDPIDLDLYFER
                     FINPQRTSPPDFDIDFSWNERDDVIDYIFNRSTFGKEHVALLATYSTFQRDAAIRELG
                     KVFGLPKAEIDSFFENGNTKRDHISNLVLQYAQRIIDVPSHLSIHAGGILISEKPMTY
                     YTALDNPPKGFPLTQFSMLEAEDVGLYKFDILSQRGLGKIKDAVAVIEKNRQVKVDIH
                     DIKKFKEDEGVRQKLQQANLMGCFYVESPAMRMLLKKLRAKTYLDLVAASSIIRPGVA
                     QSGMMREYILRFHEESRRTYIHPMMEELMRETFGVMVYQEDVIKVAHYFAGLTLTEAD
                     MLRRGMSGKYRGRAEFEKVKDAFFNGCRKKGHADKIAEEVWRQIESFAGYAFSKGHSA
                     SYAVESYQCMFLKTYFPLEYMVACVNNGGGFYAAEFYVHEARMCGGTIHAPDVNKSFY
                     ETSLYGKNIYAGFHLVHELEMLTVTNILKARAAEGQFISLADFMQRVEISLEQLRLLI
                     RVNALRFTGRTKQQLLWDIHEIMGHDKKTNTEKQLFEAEHKHFVLPELHYSKIDDVWD
                     EIELLGFPLCSPFDLIKQKTNVTEGIVIHTAAELSAYCGKIISIAGYFVTRKPTRTKH
                     GQPMAFGTFLDSKGQWIDTTHFPDVVKQYPFRGRGCYRITGKVVQEFGFYSIDVISLY
                     LLEYKTRYAVVEEDKVHVNE"
     gene            38767..41784
                     /gene="secDF"
                     /locus_tag="PKX59_00745"
     CDS             38767..41784
                     /gene="secDF"
                     /locus_tag="PKX59_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOZ89419.1"
                     /translation="MQSKGAIKFLAILVSIICLFQLSFTIVTNRIESKAHEATNGNPE
                     AYKAYIDSISQLTAYNLGFKKYTYQECKEREINLGLDLKGGMNVTMEVSVTDLIRNMA
                     NNPNDPMLVKALDDAHQAQRTSQKGLVQLFGEAFERNNPGAKLAALYSTMDLQNRVNF
                     NSTNAEVIKVIQSEADAAISRTYNILRTRIDKFGVTQPNIQQLGGGRILIELPGVKEP
                     DRVRKLLQGTAQLEFWETYDNAEVFPQLEAVNKKLAALETPAPTDSTAKSDTTVAATG
                     DSLTAAADTGAMKKPALLSKLESDTAAAKDTSSSAQFAKFSKENPLFALLTPAIFTNA
                     QNQSEYRKGPVVGYAAIKDTGKLREILERPEIKNILPRDLRLLWTAKPPTKESKFLEL
                     VAIKVKSRDGKAPMDGSVITDARQDFGQFNSSPEISMSMNPEGAKTWKRLTGENVGKS
                     IAIVLDNYVYSFPNVQGEIAGGRSSITGNFEISEAQDLANILKAGKLPAPARIVEEAI
                     VGPSLGEEAIQNSWMSFAGAFVVILLFMGFYYMRAGWVANIALFANIFFIMGVLASLG
                     AVLTLPGIAGIVLTIGLSVDANILIFERVREELKAGKGIRLAVSDGFKHAMPSILDSN
                     ITLLILGIVLYIFGSGPVQGFATTLVIGVLTSLFSAVLLSRLIFEGMLDKNKPISFGN
                     EFSNNIYKNIHFNFVDNRKKYYILSSIVIIAGIVSFAIKGLNYGVDFEGGRTYTIQMD
                     KSISTEDIRMALEPQLVEAPEVKSVGTTDRFKITTAYLIHDTASNVENVIVDKLYKGL
                     TPLYGSAPDFETFKTKNIISSQKVGPTIADDIKQSAVWAVLISCALMFVYIFIRFKKW
                     QYGLGAVVALFHDVLIVLSFFTLLSGVIPVALEIDQHFIAAILTVMGYTMTESVVVFD
                     RIREYLGHHHTADDKHVINEALNATLSRTINTTLTVFFVLIVIFIFGGEVIRGFIFAL
                     LIGRIIGTYSSLCISTPIVIDLGGNAHEGVAKK"
     gene            complement(42078..42704)
                     /locus_tag="PKX59_00750"
     CDS             complement(42078..42704)
                     /locus_tag="PKX59_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89420.1"
                     /translation="MKKHKQLSNLSTFAFIIAFLLTSCSSIIGTNKLKQVDEKTILHY
                     AKKYNINSTENYELDTAYFTFLKSFDTKLFKQQIKNHYPPLQALYYNKTGQLQSFQIN
                     CYAGGFPNLKWNKNGIIKTFPPKQQAPLDTIISLKTQLKFLKPLSNTEKSTTNDYYYI
                     VIIYWSKFMGRQSKRLIGFVQDNVKLATDKRVKIIYVNTDNFFADNLK"
     gene            complement(42747..43976)
                     /locus_tag="PKX59_00755"
     CDS             complement(42747..43976)
                     /locus_tag="PKX59_00755"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOZ89421.1"
                     /translation="MNLIIKIASRYLIGKKSHNIVNLVSLVSIAGVFTGTMALIVVLS
                     VFNGFGNLVISLYDTFDPDIKITVIKGKTFNSDALNLEKIRAIDGIKYAAFTLEENCL
                     ARYGEKQDIVTLKGLDNELMKKTGLNKAVVIGLPILENGERNFAIVGSGVAYALSVNA
                     DTGNEPLMLYLPDKNITSPGDFENALRQMNISIGGIFAIQQDFDSKYILAPMSFVQEF
                     IAAEGKASAMEIMLKPGADETTVIQKLKEISGHDFTVKNRAQQHEFLYKILSSEKWAV
                     FLILSFIMVIGIFNILGTLTMLVIEKKKDIIILKNLGAGFTMVKKIFFMEGLLITFVG
                     VVGGLLAGGIICFLQQHFHLIKLGNADSFVVEAYPVQMQVTDFVSVFLVVFCIGALAS
                     YFIAAHLVKKQYAETGK"
     gene            44120..45247
                     /locus_tag="PKX59_00760"
     CDS             44120..45247
                     /locus_tag="PKX59_00760"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOZ89422.1"
                     /translation="MKKLHMVDLISQYQKIKPEVDKAISDTVTSSAYINGPEVKAFQA
                     ELEKYLNVGHVINCANGTDALQIAMMALNLQPGDEVITASFTYVATAEVIALLKLKPV
                     LVEVIPGTFSIDPVAIEKAITPKTKAIVPVHLFGQCADMEPIMKIAAKHNLYVIEDVA
                     QAIGADYTFSDGRKQKAGTIGTIGCTSFFPSKNLGCMGDGGAIFTNDAELAKKIRMIA
                     NHGQSVQYVHDEIGVNSRLDSIQAAILRVKLKHLDSYAAARQKAAAYYDNAFRGSTKI
                     KTPERSKNSTHVFHQYTLVLDGVNRDELRHYLAEKEIPAMIYYPIPLHMQKAYKDDRY
                     KEGNFPVTEKLCANVLSLPMHTELDEEQLEYITRAVLEFCK"
     gene            45278..46591
                     /locus_tag="PKX59_00765"
     CDS             45278..46591
                     /locus_tag="PKX59_00765"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOZ89423.1"
                     /translation="MNIGVVGTGYVGLVTGTCFAEMGNHVVCVDIDQAKVDSMKQGKI
                     PIYEPHLDVLFERNIRQNRLSFTTQLTDAVKHSSIIFLALPTPPGEDGSADLSYVLGV
                     ADQLGKMLDGYKIIINKSTVPVGTAEAVRDAIAKNYKGEFDVISNPEFLREGFAVDDF
                     MKPDRVVIGTRSEKAKKIMGELYAPFLRQGNPIYFMDERSAELTKYAANSFLATKITF
                     MNEVANLCEKVGANVDAVRIGIGSDDRIGKRFLFAGIGYGGSCFPKDVQALEHTSGKY
                     GYDFSILQSVMSVNNKQKMLLAEKALEYYGGNVKGKRFALWGLAFKPDTDDIREAPSL
                     YIIDMLTKAGATIRAFDPEAMNNVKKLIGDKIEFANDEYHALEGADALLIATEWSAFR
                     TPDFDKMHSALKSKVIFDGRNLYEPQKMQEAGYYYNSIGRQTVKP"
     gene            46588..47559
                     /locus_tag="PKX59_00770"
     CDS             46588..47559
                     /locus_tag="PKX59_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017256848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ89424.1"
                     /translation="MTKRILITGAAGFLGSHLCDRFIQEGYQVVGMDNLITGDLNNIA
                     HLFKLREFDFYHHDVSKFVHVPGKIDYILHFASPASPIDYLKIPIQTLKVGSLGTHNL
                     LGFALAKKARILVASTSEVYGDPLVHPQNEDYWGNVNPIGPRGVYDEAKRFQEAITMA
                     YHTYHKLETRIVRIFNTYGPRMRLNDGRALPAFVGQALRGEDLTVFGDGSQSRSFCYV
                     DDLVEGIFRLLHCDYAQPVNIGNPVEITIKQFAEEIIKLTGTTQKIIYKPLPQDDPKQ
                     RQPDITRAREILGWEPKVSREDGLKITYEYFKSLPPEELKRSAHRFA"
     gene            complement(48302..49261)
                     /locus_tag="PKX59_00775"
     CDS             complement(48302..49261)
                     /locus_tag="PKX59_00775"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOZ89425.1"
                     /translation="MSNTKSNNIKLGFFVMSGLIVLVITLYMIGSKRSVFSKTIEISA
                     VFHNVNGLMPGHNVRYGGIDIGTVQKIIFESDSAITVKMIIEKKMAPYIKKNAIASIG
                     TDGLMGNKLVNINSLPAPSSPIEKGDVLLSLRPVESDEMIRTLNKTNDNLAAITSDLK
                     GLVQRFNKNNNLITLISDSAVVENLRQSIQSINSATNNIDNFTKDINNITKDVQNGKG
                     VLGKLIADSISENQLAILLTNLQQFSDSLNEATNNINQFSGLLVNGQGTVQALLTDTI
                     MKNDFNETLNQLKKSSFLLEEDLKALQKNFLFRKYFRQKEKRK"
     gene            complement(49258..50007)
                     /locus_tag="PKX59_00780"
     CDS             complement(49258..50007)
                     /locus_tag="PKX59_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ89426.1"
                     /translation="MMDNTNIILKIEGLCKAFGSNVVLNNFNLELIKGENIVVLGKSG
                     SGKSVLIKCIVGLLKPDKGNIHVFDTNMLELDQESLDKIRIKIGFLFQNSALYDSMTV
                     RENLEFPMRRHWISLTSSEVKNLVEESLDNVGLLHAINMMPSELSGGMRKRIGIARTL
                     IMKPKLILYDEPTTGLDPITSREIVQLMLDLQKKYHTSSVIISHDMSVAKLTANKIIV
                     LLDGRNYAEGTYDELKKSSDKNINQFFEFAS"
     gene            complement(50007..50777)
                     /locus_tag="PKX59_00785"
     CDS             complement(50007..50777)
                     /locus_tag="PKX59_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ89427.1"
                     /translation="MKLPKGISDLLHETGQLSSFVVRFFKEGFKPRYEWEELFKQAFI
                     NGYKSLPLVGITAFIMGLVLTVQSRPTLAEFGATSWLPAMIAISIVREIGPVITALIC
                     AGKIGSSIGAELGSMKVTEQIDAMEVSGTNPFKFLVVTRVLSIAFMLPILVILADLIA
                     IYACYIGVNFKDVVSWKLFYNKVFNSLEYSDFIPALVKSFFFGLTIGLVGCFKGYNSQ
                     KGTEGVGHAANSAVVVASLIIFILDLIAVQITDLLGLN"
     gene            50928..52490
                     /locus_tag="PKX59_00790"
     CDS             50928..52490
                     /locus_tag="PKX59_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOZ89428.1"
                     /translation="MADKEITQPIQTDSGIEVKQVYTSCKPMDELPAKFPFTRGVQAD
                     MYRSKLWTMRQYAGFSTAEESNKRYHYLLKQGTTGLSVAFDLPTQIGYDSDHELSEGE
                     VGKSGVAIDSLRDMEVLFDGIQLQNITTSMTINSTAAVLLSMYIALAKKQGADLSKIS
                     GTIQNDILKEYAARGTYIYPPKPSMRIITDIFDFCSREVPKWNTISVSGYHIREAGST
                     AVQELAFTLADGKAYLKAAIDAGLDINVFAKRISFFFNAHNNFFEEIAKFRAARRMWA
                     SITKELGATDPKAMMLRFHTQTGGSTLTAQQPQNNIMRVTMQALSAVLGGTQSLHTNG
                     FDEALSLPTENAARIALRTQQVIAYESGVTQTVDPLGGSFYIESLTDEVEAKAWDYIR
                     KIDDMGGAVAAIEAGYIQNEIAESSYNYQNAIQTGEKVIVGVNKFQVEEPVFDDIFSV
                     DDTIRKVQVQKLAQLRTERNNEKVNQALSNLESAAKDNANLMPYIIQCVEHYTTLGEI
                     SDKLRKVFGEYR"
     gene            52539..53279
                     /locus_tag="PKX59_00795"
     CDS             52539..53279
                     /locus_tag="PKX59_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89429.1"
                     /translation="MKKRILITLAVSTLFFTACKKDKDSKPAESELSFHLHTLVGNTA
                     ANYTDIFTDNTGRKFNIADCRYYISNIVLIKDDGTEFPLTDKALLASPSQKEYSLGNV
                     PVGKYKGFRFLMGLDSVTNHDDPAGYAASNPLSYQNPTIHWGWNNGYIFMEFVGKFDS
                     TVAATGNPVADYFYHIGFDDLKRTIDFSNSPFEITTDAGLEIGILFDLKKVLNNVDLR
                     TEFGTHTMDNMPLAVKIANNWQGAYSIE"
     gene            53313..54077
                     /locus_tag="PKX59_00800"
     CDS             53313..54077
                     /locus_tag="PKX59_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89430.1"
                     /translation="MKSIKSVLCLAVVGFIFSFSSCKKDDSDANNIPNTDTIGELSFN
                     FRQFVGNDRIVYSQVYNDTSGRPFKITNLRYYISNIVLIKANGLELPLSGKVLLVDNE
                     KEQYTLGEIPVGDYSGLRFMVGIDSATNHLDPALYDASNPLSYQPVSMHWGWNFGYIF
                     MKVEGNVDTDSVPTGNLNGIISYDIGSDPLVRVINFNHIFSVHGNINTVNFKFNILKM
                     FSGIDLATESITHTNPGRDLADRIASNWLLAFTIEP"
     gene            54074..55084
                     /locus_tag="PKX59_00805"
     CDS             54074..55084
                     /locus_tag="PKX59_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOZ89431.1"
                     /translation="MIKIQVSKIFILSVIVFWIVSIVSCRKDDAIGTGNEVLELEVPL
                     GFPYPSIPEDNQPTKNRIALGKILFFDPILSRDSTISCSSCHHADKKFTDGLKVAEGI
                     EGRKTLRNSMTILNTAYQPNMFWDGGSPTLETQVSAPIEAHEEMDFNVMKVVERLNNH
                     PLYPSLFRKAYNSGPSAFTLTRAIANFERTLFTGKSKYDAFQYEKKSDALSASEKRGM
                     DIFFGERAECFHCHGGFNFTDFSFKNNGLYEVYADSGRARITTLHDDVGEFKVPSLRN
                     VALTAPYMHDGSVATLEDVVEHYNSGGKPNFNKSFLIRPLNLTQQEKEDLVNFMKALS
                     DQ"
     gene            55107..55520
                     /locus_tag="PKX59_00810"
     CDS             55107..55520
                     /locus_tag="PKX59_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89432.1"
                     /translation="MKNIALYILTFGMTVFLASCHKDDSCNAGTGGNNSIKATLLHHT
                     KVISPKVGYPDTLFIKYDTKDFPGENASDFDAYFVNSDTAISTIEITGLKCGDYYLFG
                     TGWDADISQRVKGGIPVTIAEDAGVLTKNVPVTED"
     gene            complement(55523..57148)
                     /locus_tag="PKX59_00815"
     CDS             complement(55523..57148)
                     /locus_tag="PKX59_00815"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOZ89433.1"
                     /translation="MHIALAQLNFHVGNFENNTSKIIEAIKQAEKSGAELIVFPELAI
                     TAYPPLDFLEFDDFINRCYNSIEKIAAHSQNIAVIVGCPSRNAVPEGKNLFNTAWFLH
                     KGKVQALVNKALLPTYDIFDEYRYFEPGRKFECIEFNGEKIALTICEDLWNVEDDPLY
                     IHSPMEELIKEKPSLIINIAASPFDYKHAHQRKAILQRNVNQYKIPLLYVNHVGAQTE
                     IIFDGGSLAYDASGKLVHEGPYFKEEIIYLNTKNTTLVVEKQTEKYELIYQALLMGIR
                     DYFSKMGFKKAILGLSGGVDSAIVTVLAADALGAENILPVMLPSAFSSGHSISDSEQL
                     CQNLNIKVDKMEINPLYETFLKTLKPLFGEKPFDVTEENIQARIRGTLLMALSNKFGP
                     ILLNTSNKSELAVGYGTLYGDMCGGLSVIGDLYKTEVYELCRYINRKKEIIPENILSK
                     APSAELRPGQKDSDSLPPYDILDKILFEYIELRKGPKEIIKGGYDETLVNRILSMVNR
                     NEYKRKQLSPVLRISSKAFGLGRRMPIVGKYLS"
     gene            complement(57323..57913)
                     /locus_tag="PKX59_00820"
     CDS             complement(57323..57913)
                     /locus_tag="PKX59_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOZ89434.1"
                     /translation="MRAKVSLIAIAFVAAFQFAKAQDNKFNFGLKISPTVAWFKASDN
                     LDNDGSKIGFAYGLIADINFTNNYAFETGIDVTYRGGKLKYMDIYKMKTVLQYIELPL
                     TLKLKTNEIGYMTYFGKVGFTTGINIRTDGDFKKSDINPLNMALVAGIGTQYSLGGKT
                     ALLFGITFNNGFLDVVKEKHSKATSNFLAVDLGVMF"
     gene            57947..58966
                     /locus_tag="PKX59_00825"
     CDS             57947..58966
                     /locus_tag="PKX59_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89435.1"
                     /translation="MKFSTVRQTVYLLLSTVLMLGFSCQNDPHGDKHTKDTMTTHLKF
                     DEVLFSADSINPQHVVQWKQQYPYFFNLFVNRIIHLPPTDSAALALNLQKFVGDKDVK
                     DIHARVEKVFSAADKDKLFSEIEGFLNNYKKEFGIQPVKHLVTFLSAFNYAVITADST
                     IGIGLDMYLGSDCEFYPSIGIPQYAYQRFSKDYILSDVIKGWYQSEHDVDAEKNDFLS
                     RMIYYGKQLYFTSVMAPQVQDTILTGYSKKQLEWCSNNSKQMWAFFIENKLFYSTREM
                     EYMKYIADGHATQGFPEGAPARTAQWLGWKIVSTYMKNNNVTLKELLAEKDAQRILEK
                     SGYKP"
     gene            58997..59326
                     /gene="gldC"
                     /locus_tag="PKX59_00830"
     CDS             58997..59326
                     /gene="gldC"
                     /locus_tag="PKX59_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HOZ89436.1"
                     /translation="MKKSEIKFTVSLDDNNKPVALHWNAEDSGMQGDKECKAMMLALF
                     DKKDQTTMRIDLWTQEMMVEEMQQFFFEMYMSMADTFQNATNNKDMADKMRNFARQFG
                     KEVSLIK"
     gene            complement(59352..59759)
                     /locus_tag="PKX59_00835"
     CDS             complement(59352..59759)
                     /locus_tag="PKX59_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOZ89437.1"
                     /translation="MTTHILYTEFILYVNNLQASTVFYSKLFRKIPDLNASGMTEFIL
                     SPNCKLGLMPDNGIAKIISEKMPHPNKGTGIPRCELYFYVDDIEAEYANALKCGAKLI
                     SPIADRDWGDKVCYFADNDGHIIAFAVKQNSAK"
     gene            59811..59987
                     /locus_tag="PKX59_00840"
     CDS             59811..59987
                     /locus_tag="PKX59_00840"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HOZ89438.1"
                     /translation="MKLQRYLETYDIDNIKDAKQFLKTVVDLYNSERPHMSISNFTPN
                     SIHHSKTTIKTERL"
     gene            60243..61271
                     /locus_tag="PKX59_00845"
     CDS             60243..61271
                     /locus_tag="PKX59_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HOZ89439.1"
                     /translation="MDFDALLKLLQDWTNRVPLIILGSGASVPFHLPSMWTLGDHLKK
                     SISFADADDKKQFEEFKTTFDMVGDLETTLLQLQLRPNVLKEIIFETWRLVNKHDLEA
                     YEKILNKKVEFPLADITRHLISAAQRKLSIVTTNYDRLAEYAASLSHAFICTGYTQNY
                     FGHFSKLIHQQDLTKLNGFSGQVNIWKVHGSLDWFKSKEEVDFHLPLRQTIPTDFKPS
                     IVTPGLSKYLETQLEPFRTIFTEADSEIEKAVSYLCIGYGFNDIHVQPKLLTQIKSGK
                     PIIVITKELTPKTKESIIDSKCKQYILIEEANKKDTRIYSSSFAGEQIVPDVSYWTLG
                     EYLKLIKS"
     gene            61275..63026
                     /locus_tag="PKX59_00850"
     CDS             61275..63026
                     /locus_tag="PKX59_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ89440.1"
                     /translation="MAIFNFQNDAAIGTVFSVDTATVIIKVDEIDKLRKLHVNHLVAI
                     KSSKAGQHLIGIINRITRKASDEEKPTEEDLGLGGFLFTENILKVNLIGTLHDKHGQK
                     ENVFKRNLDTVPEIEAQCFPIDGENITKFMTTISSVDNYKVPLTIGKYSIDEHAQAFL
                     DGDKLFQRHAVIVGSTGSGKSWCVAKFLEQIAQLPMADCILFDIHGEYSGKDFKMDGV
                     QHLRVANPSDLGKKGNLANNVLMLPYWMLTYEEMLAMLLDRSDSNAPNQAMLFSKTVF
                     TEKQKFLDAIKDKIFKDHITIDSPIPYKMENVLDELQRMDEEMVAGAKVGTEKQGPFH
                     GKLTRFIQRLEAKSQDKRLGFLFQLSEDELQIDWMNTLCERLMFGSALSKEKSGVKII
                     DFSEVPSDVLPLVIGLVARVVFTVQQWTAKENQHPISLLCDEAHLYIPERTNQDAAAE
                     LGLKSFERIAKEGRKYGVSLTVISQRPAEVNRTVLSQCNNFIALRLSNAEDQAVIKKL
                     LPDNLSGLTDVLPILDIGEALVVGDASLLPTRVIIDEPKIKPESATIKFWQEWSNDKA
                     KQDIATAVTGLRKQSKA"
     gene            63042..63701
                     /locus_tag="PKX59_00855"
     CDS             63042..63701
                     /locus_tag="PKX59_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOZ89441.1"
                     /translation="MAEEYLDIYNSKGVHLGKELRKVVHQKGMWHKTFHCWVIFRNEA
                     GEDCILMQKRAAKKESAPNKLDSAVAGHYTAGEDISGGIREFKEETGIDIDSKDLIPL
                     GIRVTVSDYKPNSINKEFQEVYFIKKSIPLESFKLPVDEVAGMVELPVKKCIALLDKQ
                     IDHFFARGIFNDDASSPNIAIIIERKITIDDFIWFIDNFHFKIMLLTSKALANEKYLF
                     I"
     gene            63701..64942
                     /locus_tag="PKX59_00860"
     CDS             63701..64942
                     /locus_tag="PKX59_00860"
                     /inference="COORDINATES: protein motif:HMM:NF016099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron reductase"
                     /protein_id="HOZ89442.1"
                     /translation="MLLENLSIEKLPYLERYVNSGSPSGFTNRYTTSEETKPFGLSPF
                     FHPLICVAPEECFKNYGKIPAFKEFEEVTGKNWLLVHPDMKKEKVFKAKNVSLTKSKK
                     IKLVPTASGRTVELFNSSTKGYFKLHYTHVIGRITRELDNKIGVAGPEISKHLVSLID
                     QNKLDSRLTLMRETGARIFQGHVNEKPVEWGMVWREKDAYKIDYNEVKYVIPVFSLFG
                     KDILAKRDKPLLQQIINLIGANPTDYVLKQLIFPMIELYFNLIKNSGIQPEWHAQNLL
                     IGFTKDFAPVKFIMRDLESMDKDLTFMEEHNIKAKFESYPFKCIFREQWNYQRKHSFM
                     YDYKFGEYIIRPLLDFLKKKYKVDTLPLHQKIKLHSKKFINEFSKDFFPEYWVVFRKI
                     LINQKTDKRPYVHRKNPKFRY"
     gene            64943..65611
                     /locus_tag="PKX59_00865"
     CDS             64943..65611
                     /locus_tag="PKX59_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOZ89443.1"
                     /translation="MHIVFIRHTESEKNVNNQFSSYKDKEPLTEIGMSQSENLGNDLL
                     EFKERKNLTCKNVYAASSTRAIETAKIIASKLNATVSVEDALRSTKPGALSGKSEEEG
                     LKTNPDFLEQLYLFRNGLFNAYDFTVAEGKEPKKDFEKRVWGRLSEIISDKSESLKII
                     SAHRSSITYMLLEFAKRYYKYPQNMSGHVLLDLGKISLVETVAENKWKITRVNFDSSE
                     LKTL"
     gene            65615..66694
                     /locus_tag="PKX59_00870"
     CDS             65615..66694
                     /locus_tag="PKX59_00870"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89444.1"
                     /translation="MSENKTNKHSYLSALIQLGKFAWGIFIGISILVTVATMVTDFKM
                     TKEHPVLTGLAVLAVTIFISLILLVIKMINEGVEQKSPLPLSTELVNIINTLYEKEKY
                     LDVVRLGSALSRYLWLKGKYHERIEIGERVEDAAGKESRIEEQVSSLIDDIGWTYNII
                     GQPDKAVENITSGIDKALTNELFYYAAKGERHLSGIEKHKGHQAECLSHLQKAKEHSE
                     KILDLTSKNEMEASLFLAEAKYLFELGKLNEAEALAEKAIAVFKNDPDRIVKIHSLLG
                     NIYLKQNKLQKAKDEFNKGYNATKSTREDEVAKNLKGLADIALLERDIKSGLKYLNEA
                     KEIYLSIHKNKEATDLDNIIRSIRI"
     gene            66715..67344
                     /locus_tag="PKX59_00875"
     CDS             66715..67344
                     /locus_tag="PKX59_00875"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HOZ89445.1"
                     /translation="MPSLILTSDDFGLSKIFNQRMIELLQSNFLSSISIMINQVTSDQ
                     QNQIAEIKKMYAKNNFSLGLHLEVSEADDEATYQSQWNMFEQLLSIPPDYIDVHKGHF
                     QNATFNSIAEFCLQKRVRFRKYKETTVNVSSPESSLTATFKELGAIKEWIANFKTNST
                     YELVFHIGSFDPDSKSSLNKDRELDIEKLKHVHEFISDKNISITNYKSL"
     gene            complement(68062..68472)
                     /gene="arfB"
                     /locus_tag="PKX59_00880"
     CDS             complement(68062..68472)
                     /gene="arfB"
                     /locus_tag="PKX59_00880"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOZ89446.1"
                     /translation="MGKIDFKCLFSEVILTYSRSSGKGGQNVNKVSTKAELAFDIENS
                     VCLDEEQKKVITEKLCRRINEDGFLKLQSSEARSQLGNKELVLKKFQMLIEKAMHRPA
                     KRISTKIPAQVKEERLTQKRRTAEIKKLRQKNVD"
     gene            complement(68472..69254)
                     /locus_tag="PKX59_00885"
     CDS             complement(68472..69254)
                     /locus_tag="PKX59_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOZ89447.1"
                     /translation="MSTLRVTTVQTKLHWESQAKNIKHFTTLLKSIKAGSTDLIVLPE
                     MFSTGFSMNASVLAEKNDGPTMQWMQKMAETKNAVVCGSLIVEEGGRYYNRLVWMTPG
                     GAHAHYDKRHLFTMAGEQKFYHPGNKKLILLYKGWRLSVFICYDLRFPVWMRNQNEYD
                     CAIVVANWPEKRSFAWKQLLTARAIENQSFVVGVNRIGKDGNDITYSGDTVVLDPFGK
                     KISKTKSATENIETVKLDFDVLENARHIFPVGNDADSFTIKL"
     gene            complement(69309..70472)
                     /locus_tag="PKX59_00890"
     CDS             complement(69309..70472)
                     /locus_tag="PKX59_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HOZ89448.1"
                     /translation="MSPNQALIKSKLPKTGNSIFSVMSALANECNAINLSQGFPDFAI
                     SPELISLVNKYMKKGMNQYAPMPGIMPLREEIAAKIEARFSAKYNPETEITITAGGTQ
                     AIYTAIASVVREGDEVIIFDPAYDSYAPAVVLAGGEPVHAELKMPDFHIDWNEVKKLM
                     NQRTRMIMINTPHNPTGAILSVKDFQMLEKITAKTDIFVLSDEVYEHVIFDGYEHQSV
                     ARFPKLAERSFIVYSFGKTFHTTGWKMGYCIAPAKLSAEFRKAHQFMVFSANTPIQYA
                     YAEFMKDAKNYSGIEKFYQEKRDNFLKLIQGTAFKPLACSGSYFQLLDYSKLSNEKDM
                     DYAVKLTKDYGVASIPVSAFYRNGTDHKLLRFCFAKSEETLEKAAERLHKVEK"
     gene            70733..72487
                     /locus_tag="PKX59_00895"
     CDS             70733..72487
                     /locus_tag="PKX59_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOZ89449.1"
                     /translation="MKFPSINFSVYGKRTYLVLVSVLIGALTTLCAALLKKGVMLMEH
                     IAEHLVFDSGYHQLFLILPAIGILLTVTFWRLMKIKNERRGMTAVIYNISKQSSVVAR
                     HKLFSQMVSSIFTVGFGGSAGLEAPIASTGSAIGSNIASKLKFSTKERTLLLACGAAA
                     GISAIFNAPIAGVVFALELLLIDMPVPVVIPLLISSATAAFLSRYFNLGQPFVLITDT
                     WNSSHILYYLALAALAAIISIYCIKIYFFISSLFSKITNVFVKALIGGTLLGLLIFLF
                     PPLYGEGYDSVQLLLHNQSAEIFKNITFFEANALWLPILAALLMFFLKVVATSFTVFG
                     GGNGGMFGSSLFTGAMLGFTFSRVVNIMGWAELNEINFTVIGMASMLSGVLQTPLTAI
                     FLIAEITGGYALFVPLMITTSITYLATRYVEPHSVYTKQLALKGGLINLSKDKQVISK
                     MKLRDYLETDLETININATLGDLIKAIRKSRRNIFPVVDAMNHLQGIILLDDVKSIMF
                     DQSQYDKGVKELMQRPPLVLDIDEDMERVLEKFEEYEAWNFPVTEKGVYAGFVSKSKI
                     FTFYRQLLIQESDVPMHD"
     gene            complement(72547..72909)
                     /locus_tag="PKX59_00900"
     CDS             complement(72547..72909)
                     /locus_tag="PKX59_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89450.1"
                     /translation="MMNNSMIESTTGAEMVEAKKAAPKKKAAKKKAATKKKAATKKKA
                     APKKKATKKKAATKKKATKKKAATKKKVVSAKKAAPKKKAATKKKAAPKKKAATKKKA
                     APKKKAATKKKAAAKKKK"
     gene            complement(72974..74221)
                     /locus_tag="PKX59_00905"
     CDS             complement(72974..74221)
                     /locus_tag="PKX59_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOZ89451.1"
                     /translation="MTDLFDKISKNRGPLGMHAKESHGYFTFPKLEGEISNRMIFRSK
                     EKLVWSLNNYLGLANHPEVRKIDGEASVHYGLGTPMGARMMSGETDRHTLLEQQLAAF
                     EQKEDAILLNYGYQGMVSAIDCLVDRHDIIVYDSESHACIIDGVRLHMGKRFVYPHND
                     IDNLVKQLERATKLAEETGGAILVITEGVFGMSGDQGKLKEIVALKSKFNFRLFVDDA
                     HGFGTMGKTGAGTGEEQGVQDGVDIYFGTFAKSMALIGGFIASDAQIIEYFKYNMRSQ
                     IFAKSLPMPLVEGAIKRLELLKNQPQLKNNLWKIVNALQNGLKQAGFDIGKTQSPVTP
                     VYLNGTIPEATKLIYDLRENYDIFCSMVVYPVVPKGIIMLRLIPTAVHTLEDVKYTIE
                     SFGKIKSKLLNGEYKAEKIVDVN"
     gene            complement(74802..75101)
                     /gene="raiA"
                     /locus_tag="PKX59_00910"
     CDS             complement(74802..75101)
                     /gene="raiA"
                     /locus_tag="PKX59_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOZ89452.1"
                     /translation="MKIKVQSIHFDADKDLIRFVEEKVDKLKQFYEHIIDGEVFLRLD
                     KNVTQENKIAEIKISTPGKVLFASEQCATFEQAADKSVEALKRQITKHKEKQRGS"
     gene            complement(75216..76103)
                     /locus_tag="PKX59_00915"
     CDS             complement(75216..76103)
                     /locus_tag="PKX59_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOZ89453.1"
                     /translation="MPYAKFLRFLQYEKRFSQHTLTAYGTDLKQFFDFMGQTYQAQKP
                     EDITHFFIRSWIVSLLDRKIDSRTVNRKISSLKTYFKFLMREKTISQNPMLKIVSPKT
                     AKKLPVIVDRQKMDQLLDGVAFKEGFEGIRDKLIINMFYQTGMRLAELIGLKDKNVDL
                     YNLTLKVLGKRNKERIIPITPEMKEAIVVYLNERNNIIDEKIKSEYFFIKGDGNKLYP
                     KFVYKVVNVMLSQVTTNKKRSPHVLRHTFATEMLNSGAQINAIKEILGHSSLAATQVY
                     THNTIDKLKNIYKQAHPKA"
     gene            complement(76199..76402)
                     /gene="rpsU"
                     /locus_tag="PKX59_00920"
     CDS             complement(76199..76402)
                     /gene="rpsU"
                     /locus_tag="PKX59_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOZ89454.1"
                     /translation="MIIVQIKDNEPLDKALKKFKKKFEKTGVVKQLRSRQAFEKPSVA
                     RRTEVKKAIHRTKLQRLEAEGAM"
     gene            complement(76578..77852)
                     /gene="purD"
                     /locus_tag="PKX59_00925"
     CDS             complement(76578..77852)
                     /gene="purD"
                     /locus_tag="PKX59_00925"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOZ89455.1"
                     /translation="MNILLLGSGGREHAFAQKLSESALLKHLYIMPGNAGTLQLGTNI
                     SGSVNDFDHIKKTVLEKKIDMVIVGPEEPLVNGIHDFFLNDSQLKSVPVIGPESSGAR
                     LEGSKDFSKAFMLRNNIPTARYETFESSQLQQGYDFLKTLSAPYVLKADGLAAGKGVI
                     ICETETEAREQLHHLLINQPFGKAGNKVVIEEFLKGIELSVFVLTDGNNYCLLPEAKD
                     YKRIGDNDTGLNTGGMGSVSPVPFADAAFMQKVEDKIIRPTINGLKHENISYTGFLFI
                     GLMNVNSNPFVIEYNCRMGDPETEAVFPRIKTDFIELMNSVANKKLHEVQLKTDDRTS
                     FTVMMVSGGYPETYEKGKIISGLEKVKGVNVFHAGTIIHDGKVLTNGGRVLAVNALGK
                     NLAEAREKVYGQLTEITYEKAFYRKDIGKDLM"
     gene            78061..78513
                     /locus_tag="PKX59_00930"
     CDS             78061..78513
                     /locus_tag="PKX59_00930"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HOZ89456.1"
                     /translation="MNSLFNKQDNDRIIERIAALQPGQRALWGTMSVEQMLLHCQQPF
                     KVVFEELKLKRSLVAKLFGGIVKKSVMDDKLFKENLPTAAEFKTNHLSPQFETEKKNL
                     IAYIQKFQSAGPKGVTKLPHPFFGKMTDAEWDKLQCKHLDHHLRQFGV"
     gene            78510..79070
                     /gene="lptC"
                     /locus_tag="PKX59_00935"
     CDS             78510..79070
                     /gene="lptC"
                     /locus_tag="PKX59_00935"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOZ89457.1"
                     /translation="MKFRFVPVVFIISLLMMSCENDIEQVKLVTGNVVEPIESAKGLE
                     ILYSDSGKVKVRIAAGEMNRYLQPKPITEMPKGVKIDFFDDNLKVTSTLTSDYAVRDD
                     FNKNMEAKKNVEVVNVKGEKLMTEYLKWDEKTGLISSPEFVKIITADEIIYGNGFESN
                     QDFSRYKIFNIKGIINLKKNENTENT"
     gene            79048..79245
                     /locus_tag="PKX59_00940"
     CDS             79048..79245
                     /locus_tag="PKX59_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89458.1"
                     /translation="MKILRILEISWLVIAIGSLCAGIYKLISAGLNQAVFIFAISIIA
                     FVFFMIRRKQRIAMEKQNASR"
     gene            79283..80566
                     /locus_tag="PKX59_00945"
     CDS             79283..80566
                     /locus_tag="PKX59_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOZ89459.1"
                     /translation="MISDFLPAILASLAFVAFYSGIEIAYLSSNKFKIELDSKQGKWS
                     GKILSYFSKSPSRFICTILVGLNISLVIYGTNMSGLLNPYLQQWLPQQLNSEIGRLII
                     ETLITTFFLLLAGEFLPKILFSINPNDTLNFFSGFIWISYILLFPLVWLILKLAHFLL
                     RNVFKVDFVEETSGFGRVDLDNFIEEISENTGGKSEHSKEIQMFQNALDFDSLKVRQC
                     MIPRNEIISLSSKESVETLRKKFVETGLSKIMIYQDTKDNIIGFVHSYEMFKNPESIM
                     SVLLPVSIFPETLPARELLTHLTANHRSVAIVVDEYGTTSGMVTIEDIIEEIFGEIDD
                     EHDIDVYLEQKISETEFLFAGRLEVDYLNQKYNLELPESELYTTFAGLIFNHLEDIPH
                     VKEQFTIGSYKITVEQVSGNKIEQVRLVILVNAEN"
     gene            complement(80575..82476)
                     /locus_tag="PKX59_00950"
     CDS             complement(80575..82476)
                     /locus_tag="PKX59_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOZ89460.1"
                     /translation="MSDILHHECGVAMIRLLKPLDYYHKKYGTPFYALNKLYLLMEKQ
                     HNRGQDGAGVANIKFDAEPGTRYISRYRSNDKHAIQDIFSRIYNKFKADVNDDVSKLN
                     DTAWMKKHLAFSGEVWMGHLRYGTYGGNGIEACHPFLRQNNWMTRNLVVAGNFNMTNV
                     DELFENLIRIGQHPKEKADTVTVMENIGHYLDEENERLYYSFKEKGLSKQDISPLIAE
                     HLDVESILKQSSKKWDGGYVICGMMGHGDAFVLRDPAGIRPAYMYADNEVVVVASERP
                     AIQTAFNLSFSDITEIKPGHALIMKKNGNISMPCIKEPVERKSCSFERIYFSRGSDAE
                     IYQERKELGRLLCKSILPAVDNDLRNTVFSFIPNTAEVAFYGMMQGMEDYLREVKKKK
                     IKSLGKEISDEKLNEILSIKPRFEKIAIKDAKLRTFITNDDDRSDMVTHVYDITYGTI
                     RKGIDNLVVIDDSIVRGTTLKNSIIRMLDRLGPKKIIIVSSAPQIRYPDCYGIDMAKI
                     GDLCAFKAAIALLEENNMEHIIEETYMHCKQQLELGVNEMKNYVRDLYKPFSPEHLSA
                     KITSMLKPEDCKADVEIIFQKIEDLHIACPHNKGDWYFTGNYPTPGGNKVVSKAFINY
                     MQGINARAY"
     gene            82920..83861
                     /locus_tag="PKX59_00955"
     CDS             82920..83861
                     /locus_tag="PKX59_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89461.1"
                     /translation="MNKIFAILLLTSGSCIGQNLVPNDGFEIHTSCPYVGNSFSTLSY
                     WFNPSLNLGWGSTPDYYNGCSTLLNDVDVPSNWAGYQFARNGVAYVGIVLWHNQLTFR
                     EYFSVQLISPLVAGTSYHFEMYFNLANQFKYSCSNIGVYFSDTLYTSTINYDPLPFNP
                     QINNINSNYPDTINWSMVSGNYVAHGGENYIIIGNFQNDSNSYPILVDSNAFESGTYV
                     FIDDVSLTVYTGITEQEKIEVIKVYPTLFADKLYLSCEVNESCCVILYDETFRKIIQL
                     PFTKNASLNTGHLAKGMYFYEVRCGSSLCKKGKLVKD"
     gene            84029..84415
                     /locus_tag="PKX59_00960"
     CDS             84029..84415
                     /locus_tag="PKX59_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOZ89462.1"
                     /translation="MKHEDVTRRIIASAMKVHSTLGNGFQEVIYQRAMELEMPFENLF
                     FKREMEMPVHYRNEIIGTRRVDFFVEEIVMVELKAIIKLEDVHLAQAMNYLEAYNMEI
                     GLLINFGSKSLEFKRVHNNKPILKSN"
     gene            84469..85419
                     /locus_tag="PKX59_00965"
     CDS             84469..85419
                     /locus_tag="PKX59_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89463.1"
                     /translation="MRKQICVIILMLTCRICDAQNLVPNGDFEQYSLCPDFPANLALA
                     LFWFNPSTNVQNVSGTSDYYNACANDFYPMNVPGSFTGYQVPHSDSAYAGILLFEHLG
                     VNYREYIEVQITSTLTSNTCYHFEMYISLGDIGKYTTDDIGIYFSDTVVSNINNYAPL
                     PFIPQINNVTGNYPDTTNWLLVQGDYLATGNENYLIIGNFKDDFSIDTLLYNSQGYNA
                     SYIYIDDVSLILTPCTGIEEQNLNSNIKIYPNPLSDILNIKAWGFSEIILYDVAARKL
                     LQQKFTNSTTVNTEQLAKGLYLYEVRNKNGLCKKGKVVKE"
     gene            85924..87258
                     /locus_tag="PKX59_00970"
     CDS             85924..87258
                     /locus_tag="PKX59_00970"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HOZ89464.1"
                     /translation="MTQLLFIETFVLGASAIAAIYHLVLYIQQRDKFLLFYSIHLLTL
                     SAYILFKLLSSNYNPFLPTGNLWNYITEETLQGAMVTVYVIFAAITLDVAFSRSNART
                     VMYIFFGFCILFYSYQLYNAFEYGPGVKSIQAYMVTRLSLVGIAAIALIFAWTIRTSI
                     FHRTIIIGSLVYDFSWLVSIISLSTQTTILGLNGIEIYLLGCLLDIIIFSSALGYRVK
                     TMAEEKNELVRKEAEARLAIEKTRTGIAMNLHDDIGSVLSSMSVYGEAAKRALKENNV
                     AKVDVLIDKIGVNARETMDNMSDIVWTINPVNDNGFKLFNRMESFASSVLNAKEIELK
                     FEVGKELYEKGFLMPVRQNVFFIFKEIITNCVKHSGATNVEAIFTLNGSNLLLLVSDN
                     GKGFDTKNNHLKNASGGNGLKNIKERASVLNGTIEVKSSANGTETKLEFPLT"
     gene            87349..88002
                     /locus_tag="PKX59_00975"
     CDS             87349..88002
                     /locus_tag="PKX59_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ89465.1"
                     /translation="MAVRVTIFDDNKRLLDSLSVLIDGSPGFQMAGIFENCNNLISDI
                     ERTQPDVILMDIEMPGINGIEAVKMVKHRFPKMNVMMQTAFENDENVFAAICAGASGY
                     ILKNTPPSKILECIIEVYQGGSPMSPSVARKVLGFLQQPKPDEKKETTVENYNLSARE
                     KEILACLVKGMSYKMIAEQCNITYETVRSHMKNIYEKLHVASMTEAVAKAINQKLVS"
     gene            88144..89838
                     /locus_tag="PKX59_00980"
     CDS             88144..89838
                     /locus_tag="PKX59_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89466.1"
                     /translation="MKKLLFTLMLLSSFALQAQNFTYNRFFNGGDNSAITLGELAKEV
                     IVQPDGKVISCGYSYDFACNCFHIVMFRVDVCGNIDSTFGTNGTVSYTFEDRNTGLDF
                     LMQANGKIVVVGTQAPSNASSQHNPYIARFLNNGQPDTTFGTMGTTKVIVNPACWEFT
                     HVTEVADGKYLAQNGNYIMRFDSTGAVDLSFGNSGVINYTIPSNVPLVSSWRSVQRSS
                     DKKIISVTAAWLGSGNDMRPLFMCTDTLGQLDSTFGTNGFLLDNNVILPNYQSCHIVL
                     QNDDKILSAHHNAAETEITVARYNTNGSLDTTYGTNGYVNYTAPTSPFRARDVNILSN
                     NNLLVMADAAGLDRQINTFDTNGVMTNSVTFFGNNNYNVSGSSVPLVLHVENDDEIYI
                     VSQTGGTSGMWYISSLTTTPAPTISQNFTSLSANYNLTGTTYQWYLNGNIINGATDST
                     YTFTQNGVYSVTVTIASGCDYSYSYTVTNTGIANAANLNNIKVSPNPFNDYINISNTT
                     GQLTTLTLFDAAGRTVILETTHEPDYKKQLQQLPKGMYFLTIESGSQSKHIKLLKH"
     gene            89957..92566
                     /locus_tag="PKX59_00985"
     CDS             89957..92566
                     /locus_tag="PKX59_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89467.1"
                     /translation="MHVKIFSVLICIVLLNTNSAFAQGVGINNPTPDATALLDLTSTT
                     KGLLIPRMMTFQRLSIPAPANGLMVYDITLNAFYYSDNGIWKPMDNSWQLNGNTATNP
                     VTDFIGTTDFTPLVFRTNGFEAMRITQDGRIGINQPFSPAFNAILDIQSNDKGILIPR
                     MSSPLRSNISSPPDGLLVYDSGSNEFYYSISSVWMRLLSNNTGWTTRGNAFTNPATDF
                     IGTTDNTDLVFGTFGGARGCITSAGDWGIGTIFPSAKLHVQGSYRLEDGTQGLGKLLM
                     SDATGNASWKTFEDLITGKVANGLYFNTIQQRILIGGALTENTTITNSAFDFTYDLTG
                     AGDFQVTESSNPIPSLFVSGNKGGVNDGYVGIGTATPFGRFHINNTVVTTVNTQNTYH
                     LLIGNGNTNPDLTFGASATFALAQSWNNKPLQINPLGTFVGINHISAAPIQNLDINGR
                     LAVQNGVIQKGAATAILATDLGLYQQIAGGTIRISNNAGPIKFYTDQGGASLGGTNNL
                     VSIDNSNGGGVKIHSEISGSGQALVPAARAALDVQSTTKGVLFPRMTTAQRNAILPLT
                     AADEGLMIYNLTEHCINWWDTRPNSAGVPGFWNSQCQPCMDYFEYTASSNGNNFNTQA
                     GSPVGPKTFCVTVKAGVTLGASAMSGDALNFGALPAGSKVILHNYGQIRGGGGKGGNG
                     GSERDVTVCGVDAPATAGSDGGDAIVTSAGVQVTVYNYGTIASGGGGGGGGSGGCRSN
                     GGSGGGGAGIPAGGGGLSPNTGGRASGGLCNVCTLAPASLAGANGTITNGGLGTCSQG
                     NAGGGCSSLGFNGGCGGNGGGPGVNGNTGVGLSCGTAIAAAGGNAGFALQGNGSGSFI
                     VNVTGTFLGIVSP"
     gene            92711..94249
                     /locus_tag="PKX59_00990"
     CDS             92711..94249
                     /locus_tag="PKX59_00990"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89468.1"
                     /translation="MKKKKYNTKLNLTCILISMLMVILVFNESPAQLLTNNNVLISNS
                     IQITVKGTVQNNFGTVISNSGTIDLTGNWVNNSGNNGFGISQGTVIMNGVNQSIQGTD
                     QTVFNNLSLINGTKTIDLNTTVGGANATPTGVLNCNNAVLDLNSNTLTVNNPIGTAIT
                     TSTGYILSEDVDHSSKVTWKIASTTGIHSIPFGNAGGVQIPYSFNLTTGVAGEVTVST
                     YATVPVNTPYPVSPTMVTHVRDASGADNSANTVDRFWQVDKTGNGTAAYTFTYAATEN
                     AAGGNINIRAQSWNTTNLGWDAALPGQLNPTVQSVLVPGVNTNGAWALALASNPLPIE
                     LLSFTAKSKDNKKVVCNWSTASEINNDFFTVFRSKDGINFEEVSKVDGSGTTNTQHNY
                     SFTDNNPYKGVSYYRLKQTDFNGDYTFSQIENVLITDGDIKYLVYPNPNNGNFSVKFD
                     AEQNVNAVFLITDATGRLIQSGRLDDLSGKQQINIEKVLPGFYFLQITNGDNSYLAKV
                     QVIK"
     gene            complement(95015..96559)
                     /locus_tag="PKX59_00995"
     CDS             complement(95015..96559)
                     /locus_tag="PKX59_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89469.1"
                     /translation="MKNYVTVFALLILSVKVNSQTVQPNIPTANDRVNCIVRYNNTIY
                     IGGEFDTVGTSARPYLAAIDANTGAILPWNPVPNAQVTKMIICNSKLVVLGDFSSISG
                     SLIDRIAVYDLQSGNLLNLNSIPLNLIGIGRYGLYNAGNFVYFTASDIFGSVDAVRRF
                     DLNTLLLDTIWVSSNFNVNKCELISSGNYIYAAGWFNHYLTNSTIKNLCRFDMLTGII
                     DTTFSFSFSNSDYLNQIAAQNGKLYVSGNFSAIDGLTRKGIAEIDTSGFVTSKNIYCS
                     NSLIDALTLQGNTLWVGGNSVVLGGLNRYCIAQIDISTGIATCWFTQALQGNVYMTSI
                     WASHDTIYSAPNIEGFKTFTGNPGFVNIGNDSVLCAGSSLMLNAPNGLTNYLWNTGAT
                     TSSITVNAPGSYWFSATGSGGCIVTGYRILSACTGIENQISLVPIQILPTISNGIYSI
                     QFPLQKVSGIIKLHDSSGNLVRTFSTSPLENAKQLNISSLASGIYFCSVVWESKISIV
                     LKLVKE"
     gene            complement(96875..97357)
                     /locus_tag="PKX59_01000"
     CDS             complement(96875..97357)
                     /locus_tag="PKX59_01000"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOZ89470.1"
                     /translation="MNKQVDTSKGPGVYIPPPLFYVLIFVAAVYIQKKVPILDSALHT
                     LVIKSLGIILFITALFFLFRSLRQFFITKNTIILIKPASSLQTTGIFAITRNPMYVGL
                     AIVYLGVTIFIGNWWNIILFPILILIVQEYIIKREESYLAIEFEQEYENYKKKVRRWL
                     "
     gene            complement(97393..98109)
                     /locus_tag="PKX59_01005"
     CDS             complement(97393..98109)
                     /locus_tag="PKX59_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HOZ89471.1"
                     /translation="MPLSYISNKVISKGEIVKGQEIREGIFLLIKSDFPNFDANSIIS
                     IDELNKYRRLYLTKLVEQEKGEIATIDLDVMQAIRDNSILSENIQDEIEAKLTMGQKL
                     ADKIASFGGSWTFILSFFFFLLLWIIINIWLLKSKPFDPYPFILLNLILSCLAAIQAP
                     IIMMSQNRQEQKDRQRGEHDYKINLKAELEIKLLSEKIDHLLVHQNKKLLEIQEVQID
                     YLEDLMKQLKINYVNIKKQE"
     gene            complement(98207..99538)
                     /locus_tag="PKX59_01010"
     CDS             complement(98207..99538)
                     /locus_tag="PKX59_01010"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOZ89472.1"
                     /translation="MTKRIVKIQGFIIVFLTVVLPAFSQSNPNPSTSVDAFIQSEMNT
                     QHFPGVSTVIVKDDKIVWINSYGFADVANTIPVSDTTVFLMASVSKVFTGTAAMQLYE
                     NNVINLDGDVNQYLPWLLQIPGYPNDTISFRQLMTHTSSIQDNWAAMVGYYGYPDPTI
                     TLDDCMQRYFSTSGSDYNASANFLATAPGSVFQYSNIATALNGYLVERASGIPFDQYC
                     NINIFNKLCMNKTRWYYSNFNPNDVAIPYHYSGNNYVAYPQYGFADYPDGQLRSNVTD
                     LANFMIAYLNGGTFGTNSILSPASINQMWTVQFPSLNTRQGLNWYKEVLYYNGGSAWV
                     WGHNGGESGVSTEIYLDPVNKIGLCVLSNGEGNGLNICDQLYNYALSLNASSGYSPAC
                     LTTGIAETTTDIKRKVIKIIDLLGRESPLKTNTPLIKIFSDGSAEKFFIID"
     gene            complement(99615..101198)
                     /locus_tag="PKX59_01015"
     CDS             complement(99615..101198)
                     /locus_tag="PKX59_01015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89473.1"
                     /translation="MKKLLLVIANFCFVSLAEAQVKFQKVYGSVSDEEAYSVNLTSDG
                     GYIIGGYTSNLGNGGKDALVLKIDATGNMQWTKSFGSTNDDYIMDIKQTFSGEYYACG
                     IYDDNGNGNENIFCCKLNASGDIQWFNSFNINYTVPNKNWRIHLKQAIDSTLLLASVG
                     GTAGSNTVLAKFSVAGTMLWQSSFGGYLYGSDVAELSNGDLILIGSVGGFGAGLIDDY
                     IMKTSSSGTLIWSKAIGEINNDGSYSLNILPNDGFITTGNTWNNAIGQSDAKIIKYDS
                     DGNLIWSKNIGESGRRFISQSGCLTHDNGFAAVICDVAPSSASKMTIVKIDSTGNILW
                     SKNYGGADEDNSFAVKNTTDNGFIIAGYATSFGVGGKDIYIVKTDSIGNSGCSTTQFT
                     NTPSSANWPGIVNIFSPTIGSPFPILNQIMVSTPSIAADVFICGDTTVSIKENSNLIY
                     QLSPNPTNGQFTISFDKLIPTGKVNIYNAVGEIVGCEVINNESQKLINLRNISAGVYF
                     VNVLNGEASYRKKLIVTQE"
     gene            101425..101763
                     /gene="ytxJ"
                     /locus_tag="PKX59_01020"
     CDS             101425..101763
                     /gene="ytxJ"
                     /locus_tag="PKX59_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HOZ89474.1"
                     /translation="MGFKTLDNIAQLNEIDTLSKNKKQIIFKHSTRCSVSTFAKKVLL
                     SEFSDDMEKKFDVYYLDLLNYREVSNKIANHYGVYHESPQILIISDGKCIYDASHDDV
                     SLASALASVN"
     gene            complement(101771..103105)
                     /locus_tag="PKX59_01025"
     CDS             complement(101771..103105)
                     /locus_tag="PKX59_01025"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ89475.1"
                     /translation="MPSKILLVDDEPYIEDLVRQKFRKQIKENQFEFEYVSNGKIALE
                     KLKNDDSYDLMLTDINMPVMDGLTLLEKLRENNILTKAVVVSAYADLSNIRTAMNRGA
                     FDFITKPIDLADLEATIKKTVLEMEMLRKGIEASRNLDDALLQKAEAQQKALDALLEK
                     EKLILQQNELLETQVQQRTAELQQQKLLVEEKNKEILDSIHYASYLQRAILPSEETLA
                     SFFKESFVLYQPKDIVAGDFYWIEPVEDGHIVIVADCTGHGVAGALLSMLGISLLNHL
                     VRDKNITRPAELLNQLRIEVSRSLNQAETHAAEGMDIAVCHINCSAKQITFAGAYRPL
                     VFISGNELKTLSGNKIVIGGNNILQQQEFINQTISFDDAGTVYLFTDGYADQFGGEKN
                     KKLMSGKLKELLLEKHNLPMKEQLTFFTDYLKKWRGKNDQVDDVLLLGFRCQ"
     gene            complement(103109..105352)
                     /locus_tag="PKX59_01030"
     CDS             complement(103109..105352)
                     /locus_tag="PKX59_01030"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOZ89476.1"
                     /translation="MFNLHRLFLSIFFSGICFNVIGQNSNKKSDLDSNVNIKVNLGNL
                     LSDDEDAQANADSTIQVFERNENLLEFTNNWKFKRGDDTLWKEPIYDDSVWEEYSDTI
                     RSNHIKNYTTIVWYRMHFDIDSSLLNRPLALILHLYGSAAEVYLDGRLLKKYGTVASN
                     ISEEEAVFNTNPQPVAVVFSNLNNHVLAIRYSNFHNSSARISGFNIGENFTIKFKDLN
                     SEIDDSIQTIKKSSLILFVAAVFLTLAIVHLIMFLYYRQKITNLYYSLYCIGIFIFTF
                     YTYHLLTSSNYSSIAFISKSLQFLAPLIVVPIVAMLHTIFYHHFRRNLWVLLGLYAVM
                     IVLYLLPFRKIAAILMAVIIITSFVEIFRVIIVSVRKKRDGAWIFALTLLLPVFLGII
                     ISILPDTITFWGMKFEVKTSNFISVSMLMGLPFSVALYLARDFARMGNTLTKQIDEIT
                     ELSTRTIRQEQEKKHLLENQKAMLESEVKERTHEVLEQKNVIEIKNREVTESLHYAKR
                     IQAAILPDIKKINQSLNDSFILYLPKDIVSGDFYSFAQRDEKIIVAAADCTGHGVAGA
                     FMSMIGTALLNQIVNEKGITEPSKILTQLNDGIVTSLKQRESEMNEGMDIALCTINNK
                     NKTLQYSGANRPLWLIRKNEFQFYKPNKIPIGGQQYNKADGFMQHNITLQQGDAIYLF
                     SDGFVDQFGGEFGKKFMTKKFRELLLSIQHLSMNEQKEYLLNTLIKWQGKHDQVDDIL
                     VIGIKIP"
     gene            complement(105423..106769)
                     /locus_tag="PKX59_01035"
     CDS             complement(105423..106769)
                     /locus_tag="PKX59_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOZ89477.1"
                     /translation="MTTMKKISIIGAGLVGSLLALYLNKRGYKVTLHERRPDMRKVKI
                     PAGRSINLALSDRGWRGLQGVGIDDAIRKIGIPMTHRTMHSVDGKITTQPYGKDGQGI
                     YAVSRGILNCTLLDLAEKGGVEIHFNQRCTGLTLASGEMHFENSESHQKTTFASDLIF
                     GADGAFAASRLQLQLSTEQFNYSQSYLQHGYKELMFVAKADGSHQLEKNALHIWPRGG
                     YMLIALPNLDGTFTVTLFFPLKGNPSFESLHNKQEVKTFFQKVFPDAVPLLHDLEEDF
                     FHNSTGSLVTVKCAPWSFDNKVVLIGDAAHAIVPFYGQGMNCGFEDCSVLNDLLNQYA
                     DDWQKVIPEYERMRKPAADAIGELAVDNFIEMRDLVGKPEFLLRKKIEGWFSTKHPDK
                     WIPLYTMVTFSHIPYHEAQARGKRQDAIMNEIMKMADIENIWNSSVVEKKIIDLLK"
     gene            complement(106766..108052)
                     /gene="kynU"
                     /locus_tag="PKX59_01040"
     CDS             complement(106766..108052)
                     /gene="kynU"
                     /locus_tag="PKX59_01040"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HOZ89478.1"
                     /translation="MHFINSSDFANQLDEQDPLKSFRQKFLFPQHQNKDVIYFCGNSL
                     GLQPKQTRDYVLQELNDWKDFGVEGHFQAKNPWYPYHEFLRDNMALIAGALPSETVVM
                     NSLTANIHFMLVSFYKPTTKRFKIICEGSAFPSDQYALQTQVKFHGFNPDNAIIELHP
                     RTGEHTIRHEDILETISKHKDELVLVFLGNPNYYTGQVFDMAGITQAGHAAGAIVGFD
                     LAHGIGNLELHLHDWNVDFAVWCSYKYLNAGPGGVAGAYVHQQHCNNTDLIRFAGWWG
                     NDPQTRFTMPHDFIPVKSADSWQLSNAPVLPMAALRASLEIFAEAGVQNLIKKRKLMN
                     DFLRFIIGDIISKTNSEKNIIMITPENENERAAQVSMLCKHNGKKIHGYLTEQGIIAD
                     WREPEVLRIAPVPLYNSFNDIYQFGKCLHEALLLNR"
     gene            complement(108127..108690)
                     /gene="gmk"
                     /locus_tag="PKX59_01045"
     CDS             complement(108127..108690)
                     /gene="gmk"
                     /locus_tag="PKX59_01045"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOZ89479.1"
                     /translation="MTKHFLFCGPSGSGKTSIVQHLLTKFDFMQFSISATTRPKRANE
                     TDGKDYYFMSIENFRKHIDRNDFVEWEQVYEGRYYGTLKSEVERIEALNCSVVFDVDV
                     EGGLKIKKHFGKNLLAVFIRPPSVEDLRQRLTARNTETAESLEIRLNKSIKELSYQDR
                     FDNVIINGELNQSCKEAEALVSAFLQN"
     gene            complement(108687..109559)
                     /locus_tag="PKX59_01050"
     CDS             complement(108687..109559)
                     /locus_tag="PKX59_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOZ89480.1"
                     /translation="MIRSMTGYGKASGDYKSKTISVEIKSLNSKFFDLNMRLPHPYKD
                     KEMDLRSLLLKEAERGKMDVMITMESDLAQQENLLNREIIKSLLTDLKAVKKEFALPD
                     DNLLQIVMQMPQVVNTDSDDADENEWKYIFSIINQAIEKFNLFRQREGHNLQQDISQR
                     MNLILQLLEQITPFEEGRINAVRSKLISSLKSLGGNIETDKNRFEQELIYYLEKLDIT
                     EEKVRLAEHCRYFLSMLNDENNNGKKLGFIMQEIGREINTIGSKANDADIQRKVVEMK
                     DEAEKIKEQLSNIL"
     gene            complement(109606..>110150)
                     /locus_tag="PKX59_01055"
     CDS             complement(109606..>110150)
                     /locus_tag="PKX59_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ89481.1"
                     /translation="IKYIGITLGIAGAMLLILLGKNFNLQGGNTEGDLYIFLNSVSFA
                     IFIILVKPLMLHYHPITVMKWTFFFGALMVLPFGFNEVTAVAWSSLTFNLWLCILFVI
                     VAVTFVAYLMNVFALRHLSPSVVSIYIYFQPVFATLISMALGEASPGWLHLASAVLIF
                     TGVYLVSKQKIAGINNTENS"
     assembly_gap    110151..110160
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<110161..110515)
                     /locus_tag="PKX59_01060"
     CDS             complement(<110161..110515)
                     /locus_tag="PKX59_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ89482.1"
                     /translation="MKATTRAHLSVLFVNLLYAANFSIAKQVMPEFIKPFGFIVIRVI
                     PATVLFFLAAFLIPSEKIKKKDRNTILLCALFGVAINQLLFFKGLSLTSPINGALIMT
                     TNPVLVLLMAAIILKE"
     gene            complement(110519..110896)
                     /locus_tag="PKX59_01065"
     CDS             complement(110519..110896)
                     /locus_tag="PKX59_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOZ89483.1"
                     /translation="MKKIYHLANCTTCQRILTELKPGKDFILQDIKSEPITEQQLDAM
                     QKLAGSYEALFSRVAMKYRSMGLNQIKLSEKDYKKYILEEYTFLKRPVIIIDKEIFIG
                     NAPKTVAAAKAAMHKNKNQTKNH"
     gene            complement(110943..112418)
                     /locus_tag="PKX59_01070"
     CDS             complement(110943..112418)
                     /locus_tag="PKX59_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ89484.1"
                     /translation="MRILLTGANGYIGMRLLPVLVEAGHDVTCVVRDALRFKPSPDLL
                     DKIHVIEYDFLEPENAIQQFNNKQFDVAYYLIHSLGDTATTLKEYELRCAGCFVLVAT
                     LTQVKQIIYLSGISNEDNLSKHLTARKGVKDVFINSGIAYTIFEAGIIVGSGSISFEI
                     IRGLTEKIPVMIVPRWLKSKCQPIAIRNVMNYLHLSLMNEKAFNRVFEIGGPDVLTYR
                     KMLLEYAAERGYKRLIITLPFGFMGLSATWIYFSTPANYTIAKLLVQSMKNDVVCKET
                     SVRDVIPQELYTYREAIQMAFARIEQNMVISSWTDSASSSLSRLDVNQHIEVPSNGCY
                     KDRKWVEIDKDKADEVANRFFNIGGQNGWYYADTLWRMRGIIDKLIGGVGMSRGRRSD
                     VDIEPGDTLDFWRVLLVDRKNHRLLLYAEMKLPGEAWLEFSIIHNNNRCILKQTATFR
                     PIGIMGRNYWYSMLPFHFFIFRNMIKQIAGDGQHKIRNTKI"
BASE COUNT        35677 a        20209 c        21319 g        35279 t
ORIGIN      
        1 tgttaaagct agtgtaagtt ttgaagaact gtttgagagc tgtaccagca aaatacatgc
       61 agtttttatt tttcttgcct tgctcgattt gttgcagata catgaagtaa gtatcaacgt
      121 tggcgaaggg ttcaacaatt ttgtactgca gaaaccacag gatctgttta ctcatgcaga
      181 tagagagtaa agatgttctg cgcaggctga atccaacaaa aataatttgg ccaatgctca
      241 ttggtctgtc agtggcaggt tggttgtttt atgataatta cgatggcaaa gttttttcgc
      301 aaattgactg gacatggcat agcaccttct ggatgattct tgcaggcttt tctgtatttg
      361 tgcgcgattt ggcgtacatg attcgcattc gggtacttac agataatgaa cttacatggt
      421 acagaagttt tattgtaatt atgctatggg agttttcttc tgccttagca ccgggaataa
      481 taggtggcgg atttttattt gctattttta ttctcaaccg cgaaggaata aacctcggta
      541 agagtattac caccataacc tttacctctt ttcttgatgg tgttttctta gctgtgatgg
      601 caccggtagt ttatttgctg gtaggtaagg acgctttatt ttcaggcgta aatgtggggc
      661 aaacatcatt gggtacaagc attttttatt ctttctgggt agtatatttt atcattcttg
      721 cctataaact ttttattgct tatgcacttt ttgtaaatcc atatctgatt aaaaaaatat
      781 taacgggcat ctgttctttt ccggtcttga aacgatttag aaaaggtgca gttgaaacag
      841 gcgatcaact tatcattgcc tcaaacgggt tgaaagataa aaatctgaaa tactggatgc
      901 tttcattact ggcaacgttt gcttcgtgga cagcaagata ttctattgta aattgtctga
      961 ttcatgcatt tcacgggcag accgcattgt ctgatttgat tgtttatggt aagcaggtaa
     1021 ttatgggaat aataatttta cttagcccca cacctggtgg cagtggcatt gccgaactga
     1081 tgtttaccga ttttcttggt gagtttattc acaagggact ggcacctaca ttaggaattt
     1141 tatggcggtt gataagttat tatccttacc ttattgttgg tgcactaatt ttgccacgtt
     1201 ggattagaga gcgtattgta aaaaaaatat tttagggtta tgagtaataa gcgaagaatt
     1261 tttctgattg tcatttcagt aactattgga atgggtcttt ccgtcatcat ttttttaatg
     1321 aaaaataaag gaaaagcatt aagcgatatg gactatcttt ggttaggaac caatctgttt
     1381 ttttcattgc tgattgttgg cggcatagga ctttatttta tgaagaagaa agacaatgat
     1441 ctgatgaaat aagtaagtat tagttaagta atttaaaaat caaaacaatg aaaaagcaaa
     1501 ctttagtagt agcattaaca atagtttttg gcttgatgac atttgcagtg caagcacaca
     1561 atgaaggcaa gcaagttacc taccaagcgg gcaacaccaa ttgcaatggt tatgttgcct
     1621 ataatgcaca cattccaaaa atgcctgttg tattagtcat acctgaatgg tggggttgta
     1681 atgaatatgc taagatgcgt gccgacatgc ttgcttctat gggatatttt gcttttgccg
     1741 ttgacatgta tggtgatggc tatattgcac ctacaccaaa agaagcaggt gagcaagctt
     1801 caaaatttta taccgataat gctttggcta tggagcgcat taacgctgca attcaaaaac
     1861 taaaagattt tcctatggcc gatgttgaga atatggccat tataggatat tgttttggag
     1921 gcagtatggc acttaatgca gcacgtgcta atttacctgt aaaggcagtt gtaagttttc
     1981 atggtggatt aaagggcatt gccaatccgg cagagataaa accaaaaata ttaatctgtc
     2041 atggtggcag cgacaacttt gtttctgcag aagaagtgac tgcttttaaa gctgagatgg
     2101 ataaagtaaa agcaagttac agatttattg actacccggg tgcaacgcat gcattcacca
     2161 atcctgaagc tactgcagtt ggaaaaaagt ttgatatgcc aattagttat aatgccgaag
     2221 cagataaaaa atcatggttg gatatgaaag cctttcttgc agagcatctg aaaaaataaa
     2281 ttattgcttt gaagcactta atgcatatac aagaggcaat ttgttttcca tgccttttat
     2341 cataaagtga tttttactta cttcaaccat gttgtttaaa cagttgtagg gagaataatc
     2401 aaactcatta aaagattcaa ttcttaatcc gctgtttaat agactactca ttacctcact
     2461 cagactgtga ttccaggtaa ctgatgtagt agtgattttt gcttgtttat cggcataggt
     2521 tccttcatca gtttcaataa tgggagcact tttaaaataa ctgtactgta catatttaaa
     2581 atcattatca aacatccaaa cagcaggatg aaattctgcc atgacaaaca cacctcctgg
     2641 tttaagaaaa gatgaaataa tatcagccca tttttgcata tcaggcagcc agccgatgac
     2701 accataagaa gtaaaaacaa tatcaaactt ctgatccaaa acagtattta aactatacac
     2761 atcactgcat acaaatttgc aacccgttcc tgatttttta ttcaattcta tagctgtttc
     2821 aattgctttt tcagaaaaat caacagcagt aacttgcgca cccaaacgtg ctaaagaaat
     2881 gctgtcctgc ccaaaatgac attgcaaatg cagaattgtt ttacctttaa catcaccaag
     2941 cagattgagt tcaatttcat tcaaagaatt ttttccttta agaaatgtct cgttatcata
     3001 aaatgcagaa tctacatgaa catttgtgcg ttcattccag agttttttgt tcaaatctaa
     3061 ataatccata atgtaaaaat agatgttcgg cacaaatacc ggaatattgt tcaatacatc
     3121 ttatctttgc tctgtaaatt aatcggaaga gattatgtcg cacattctac tcattgagga
     3181 caataaagaa gtaagagaaa atactgcaga gattcttgaa cttgcaggct acaccgttac
     3241 aactgccagc gatggtaaaa aaggtattga agcagttcag aaaaacaaac ctgcattaat
     3301 tatctgtgac attatgatgc ctgtgttaga tggatacggt gtgctgcatc tgctgggtaa
     3361 aaatcctgaa acctcaacta ttccgtttat ttttctgaca gcaaaaacag aacgtggcga
     3421 tttcagaaaa ggtatggaaa tgggtgctga cgattacatc acaaagccat tcgataaact
     3481 cgaactgctt aatgccattg aaagtcgttt aaaaaaggtt gaagcattaa aaaaagaata
     3541 ttcaaaagac cttgaaggac ttaccaattt tattggtgaa gttggcggca aagaccacat
     3601 gcaggcattt attgaaaaca gaaaaatcag aacctacaag aagaaagaaa atatctataa
     3661 ggaaggcgac tatccgcgtg gtatgtatta tatcaaccat ggaaaagtaa agctttataa
     3721 aagccatgct tatggaaaag agttaatcac caacctgctt aaagaaggtg atttttttgg
     3781 ttacacttct ctcatcgaag aaggcgatta tgtagaaagt gcacaaacac ttgaagacac
     3841 agaagtggtt tttatcccca aggaagattt tctgaatctc cttttttcga atcgagaaat
     3901 tgcaaaacga tttctgctga tattgaatca aaagtttaat gagcagcagg attacctgat
     3961 agaattggca tatagctctg tgagaaaacg tgtggcagaa gcattgattt tactttccga
     4021 caaatacaaa gccaatcgcg atgaacgttt cagtatgtca atttccagag aggatttggc
     4081 caacattgtt ggtacagcta cagaatcact catcagaaca ctaagcgact ttaaagaaga
     4141 aaaactcatt gaggtgaaag acggtaaaat tgtcttaatt aatgagaata aattaaagga
     4201 gttgaagaat taagcattaa ccatgcagtc aatacttaat tctattttct atttcttagc
     4261 tgctgtttcc tttattactt ttccattctt gtataaaatg gttttaagta atttaccatc
     4321 ttcgtaaacc cgccattcgc cttcttcatt gttgtttaca tagttgcctt cactttgtat
     4381 ttcgccattg gcataaaaaa tggcagggcc gtttaagtca ccattcttgt aggtatattg
     4441 tatggtaggt ttgtcgtcct gtttaaacca aatggtaata ccatcacgaa ttccgttttt
     4501 ataagttgct ttttctttca gcttcccgtt ttcgtaataa tcaaatcgca ttccgtctaa
     4561 ttttccgtta ttgtaatttt cagaggtaat tagtgtaacg ccatatttgt aaacattacg
     4621 caggccatgt aatatacctt tgcgatagga ttcctcatct tcaatgtaac cgttctcgtt
     4681 aaaatgcaga tgtagtccgt tgagcacatc agcttcaaat tcttcaagca gttctatttt
     4741 tcctttggta tcataaaaac gccaaagtcc ttctttcttg ccattttgaa tttttccaac
     4801 ggcacgggcc cttccgtcag gaaagtttat tcgacagtca aataatccgg ccttgtcggg
     4861 gtctggagtc aatacagtat cttgcttaga agtttgtgct cctgcaaatg ataatgatag
     4921 tccaagagca atggtcaaaa gtgatttaaa cataatgctt aatgttatgg cttgcaaatc
     4981 tctaaaaaaa tgatgaaaag cagattatag tagcatcagc agctaatgct tatttttgca
     5041 ggcacaatga caacacagga aatattagaa aagttagaag cgctgaaaca tcgttttgaa
     5101 gaaattggtc agcagttgag cgacccttct gccacaacag atatgagacg ctttcagcag
     5161 ttaagccggc agtataaaga attgcaagag gtagtagaaa cttatgaacg atataaaaaa
     5221 gtaattgata attacgatca ggcctatcat gttttaaata cagaaaaaga tgttgatctg
     5281 aagcagatgg caaaggaaga aatggaaacg ctggaagcag aaaaaaatag tcttgaagat
     5341 cagataagac ttttactgat tcctaaagat cctattgatg aacgtaatgc agtggttgaa
     5401 attcgtgcag gcaccggtgg tgatgaggcc agtatttttg caggcgatct ttacagaatg
     5461 tacagtcgct attttgagaa aaagggatgg aaggttgatg tgattgatga gaacgaagga
     5521 acagcaggag gcttcaaaga agttattatg gaagttagtg gcgatgatgt ttatggcatc
     5581 atgaaatatg agagtggtgt acatcgtgta caacgtgtgc ctgatacaga aactatgggt
     5641 cgtgtgcata cttctgctgc tacagttgta gtgttgcccg aagcagatga tgtagatgta
     5701 gtgataaatc ctgcagattt ggagtttcag acagcgcgct caagcggtgc cggtgggcag
     5761 aatgtaaata aggtagaaac aaaagttcag cttacccata aacctactgg cataatggtt
     5821 acctgtcaga ttgaacgttc gcagcatgga aaccgtgaga gagcattgca catgctcaaa
     5881 accaaattgt atgaaataga gttgcagaaa catgagtcgg agattgctgc cagacgcaaa
     5941 acaatggtat caacaggtga tcgctcagcg aaaatcagaa catacaatta tccgcagagc
     6001 agagttacag atcatcgcat cggtttgtca acttacaatt tgtcatcttt tatgaatggc
     6061 gatattcagg aatttattga tgcattgcag ttggccgaga atgcagagaa aatgaaagag
     6121 gcctcattaa gttaagctat gaactatcag cagttagtag gcgaaataaa aagaaaacaa
     6181 tcttttctct gtgtaggttt agatacagac ttaaaaaaaa tcccttcaca catcagagat
     6241 aaagcagacg atcctgtatt tgaatttaac aaagcaatta ttgatgccac attagactat
     6301 gcaatagcat acaaacccaa tcttgcattt tacgaagcgt caggtgttga gggactgaag
     6361 agcctcgaaa aaacaattca gtacatcaat ggcagagcat ttaccattgc cgatgcaaag
     6421 cgtggcgata taggtaacac tgctgaaatg tatgctcatg ctttttacaa tcattatggt
     6481 tttgattcca tcacactctc accatatatg ggtgcagaca cgattgaacc attcttaaaa
     6541 cataaagata agtggagcat cattcttgca ttaacatcaa atcccggagc agaagatttt
     6601 gaaatgctta aagtggatga tgattttttg ttcgagaaag ttttgaaaaa atttagcaag
     6661 gcttcagcgc aacagataat gtttgttgtt ggtgccacac gtccgcaata ttttgaaatt
     6721 gttagaaaac atgtgccaga caattttctg ttagtccccg gcataggtgc acagggtggc
     6781 agtcttgaag atgtttgccg ctacggactg aattccaatg caggattaat agttaattct
     6841 tcacgtcaaa taatttatgc agacagcgga attgattttg acaaaaaagc agctgaacag
     6901 gcaaaacaaa ttcaacaaca gatgaaacag atgcttgttg aatttaaaat tacaggctga
     6961 tgccatttac cttagcgcat cctgcaatag taattccttt ttacagactc tttaaagagc
     7021 gtctgtcgct gacaggttta attattggga gtatggttcc cgatgttgaa tattgtgtaa
     7081 acacagtaac acgaagtgta atcagccaca ctgaaaaagg tatttgggtg tttgatttac
     7141 ctgtagccat ttttctgact ttttgctacc atggattcgt caaacaaatt ttaaccaata
     7201 acctgccgga ttggattcgc tatagacttt ctccttatgc caataaaaac tggtttcatt
     7261 atttcagaaa gaattttctg aattattttc tctgtcttct tgcaggaatt gttttacatc
     7321 tgctgtggga ttctgtttcg catggaactg gatattttgt aagacgaaca gcatttttac
     7381 aagagaatgt aatcggtaca ttaacggtta cacgattaat atggcacata agtaccgttg
     7441 ccggccttta cgtagttatc cgattttttc tgagttttcc cgtcaacaaa atggaagaag
     7501 aaaataatat accagataaa aatttctggc cgttggtttt tttagttgca tcagttctca
     7561 ctatcattca ctggctgcct tttgtcagac cgcaggaacc acgttttatt ttagttgctt
     7621 tttcaggtgc aatgatggtt ggagttattg tagtatgttt tatctttagg ttttttaaga
     7681 aacaactgtc atgaatgaag cattgcttta tcatctatgg aagtacaggc tatttaagtc
     7741 tttgccgcta actactattg atggtaagac aattgaggta ataaaacccg gagaactcaa
     7801 tcccgactca ggtcccgatt tttttaatgc ccgtctgatt attgacggaa cagaatgggc
     7861 aggcaatgta gagattgacc ctacaagtgc tgattggtac aaccacaaac atcatatcaa
     7921 taaagcatat tcaaagttaa tactacatgt tgtttacgaa atgaataaac cattagatca
     7981 tggttttcct gtgttggagt ttaaaaaatt tgtggacgat ggagctgtaa aaacattcca
     8041 tcaattgcaa gaatcgttat tgccaatacc atgcagcaaa caagtacatc atgtaaacga
     8101 tgtaacgtta aacacctggc ttgaaagaat gattatagaa cggttagagc aaaaaactac
     8161 tgttattcat gacatgctgc atgctaataa gtttgactgg gaagagacat tttatttttt
     8221 tcttgcccgt aattttggat ttaaaaccaa tgcactgccg tttgaaatgc ttgcacgttc
     8281 tattccgatg aatgcacttg cacgacataa aaataactta ttgcagattg aagcattgct
     8341 gtttggacaa gcgggatttc tcaacgataa acatgctgat gaatacattg aatccctaag
     8401 acgtgaatat cgttttcttg caggtaagtt tcagttgcag gcaatgaaca atagcttatg
     8461 gaagtttgca cgaatgcatc cacgcaattt tccttctgtt agaatagctc agtttgctgc
     8521 actcatccat aacagcacac atttgttttc aaaaatattg gaagagaaag atgttaaatc
     8581 gcttattcaa cttttcagca tcacaccttc ttcttattgg gataagcatt ttcattttgg
     8641 aaaggaatct gcagagagta aaaaaacatt cggacgcagt tcagttgaca atattattat
     8701 caacactatt gcaccatttt tatttctata tggtaaaatt aaagataatg aaaaattaac
     8761 cgaacgtgca ttatctttgc tcgaacatat caaaccagag aataatgtaa taataaggca
     8821 gtggaaagaa gtaggaatta cagttaaaca tgcctatcat acgcaggcat tgattcaatt
     8881 gaaaaatcta tactgcagta agaaaaaatg tttgagttgt gctttaggag ttaaaatact
     8941 taataataag aaaaatgatt attgacagca ttaaaaattt ttttgaaaga caggctttcg
     9001 gtatctgtga gtattggggc gaacgtctaa aaataagatc atcggtcatc aggttgtttt
     9061 ttatttatct ttcttttctg acaatcggtt cacccgtagt tgtgtatatg gtgcttggct
     9121 tctggatgaa tcttagaaaa ctcattcacc gtcaacgtgg aggtgtgtgg gatttataga
     9181 gaaattttgc agtaatagat ttagtttttt acagaatgat aaaaatgtac attaagccca
     9241 cttgcagcac ctgtcgcact gcaatgcaaa tggctaagga gtgtgagaca gagatagaaa
     9301 tgatagaata cctgattgat acaccatcag aaaaggaatt gctggagtta acacaacaat
     9361 tgggagtaaa accatttgac ttagtcagaa ccaaggagtc gctatataaa gagaagtatg
     9421 aaggcaaaaa aatttcagat aaacaatggc tgaaaatttt gcataagaac cccattttaa
     9481 ttgaacgacc tataattact gatggcactc atgcaatcat aggaagacca ccaacattaa
     9541 ttgcggactt cctgaataag aagcaaaaga cgaaaaagaa atcaaagtaa actgtgataa
     9601 aaattttagt ttgtaggagt agaatgcaat acgttattca aagattattt cttttggttt
     9661 tgatgctgtc agcaattcag gtcaatgcac aacacgatga tttgtttatt ccccgaaata
     9721 ttcaaaaagc atatcagaac aatacaagaa caattgaagg tgcaccagga aaaaagttct
     9781 ggaaaaattt tgcagagtat tttattcagg taaagtttaa tcctaaaact ttaaaaatag
     9841 aaggaaagca gattataact tactacaaca attctcccga tactttaaaa aaattggtgt
     9901 ttcgtattgc ccccgacttt tataaaaaag gaaatgaacg caatcgtatt attgaaccag
     9961 acgatgttac tgatggagta aaatttacta attgtgttat tgatgcaaac aaagttgatc
    10021 ttaataatcc catgctagta aaacacgaag gaacaataat ggaaattaag gttaaaggta
    10081 ttgatcctcg tggtggcagg gcaactgttg agatggactg gagctataca ctcaatctga
    10141 actcgcatga acgaaccgga gtgattgata taggaagtta tttcattgca tatttttttc
    10201 cacgtattac tgtttatgat gatgttgatg ggtgggatga attcccttat cttggacaac
    10261 aggaaaccta cttcgacaat gccagcttta atgtccacat aacagtgcca agaaatttta
    10321 tggtgtgggc tacaggtgat ttgcaaaacc ctgcagaagt gctgcatgaa tccgtactaa
    10381 aaaaatggac tcttgcacag caaagcaaca aacctgtttt tttaattgac agtgcagata
    10441 ttcagaataa aactgtaaca gtgaataaaa aattcaacac ttttcagttt aaatgcacta
    10501 cacctgattt tgtttttgct gtaagcaatc attacttatg gcaggcacgt gatatggttg
    10561 tagatagtgt taccatgaga aaggcattaa tttcaacagc atttaatcca atacataaag
    10621 atttttttga ggtgaatgat tttacggcaa aaactattga agtgatgagt tttgattttc
    10681 cgggtgtggc atatccatac agtcatctta ctgttgttga cggacgtgat cagatggagt
    10741 atccgatgat gataaatgac aatcctacta ctacacgttt tgacggaatt acattgacaa
    10801 cacatgaagt ctttcatcag atatttccgt ttatgatggc taccaaccaa accaaatatg
    10861 catttatgga tgaaggctgg gctacaattg gcgagtggta tatcagtccg ataattgaca
    10921 gtactattgt tgacgattat ggaatggata gaattaactc cgatttaggt aaagactggg
    10981 atacaccaat gattaccaat tctactgagt tgacgaaatc attattcatt aacagttatc
    11041 ccaagcctgc tctcacattt ttatatttat gggagatgat gggcgattca ttattcaaaa
    11101 agtcattgca gcattacatc attacctgga tggataaaag ccctactcca tgggatttct
    11161 ttttcagcat gaaccaaagt tgtggaatga atttaaattg gttttggaag gcatggtatt
    11221 ttgaacaagg ctatcctgat ttagggatta ttggtattaa ccgtcttaac tatagttaca
    11281 ctgtaactat taaatctatt ggcaacaaac ctgttcctgt acatctcaca gcaacatttg
    11341 cagatggaac aaaaaggaaa tttacacgaa cagtagatat ttggaaaaac agaaacatca
    11401 ccgatgtgat aattgataca caacagtcaa ttactaagtt ggagttgggc gatgtttatg
    11461 atgctgatgt agataaaaac aataacgtgt tagttcctaa agagtgagga attaatttaa
    11521 ttttgcagac gaaaaaaatg gtcccgtagt tcaacggata gaatagaagt ttcctaaact
    11581 tttgatggca gttcgattct gcccgggact acgaagaaaa ggcagttccc tgtccgacta
    11641 cgaagtaggc ggagattctg ccagagacta cgaagaaaag gcagttccct gtccggctac
    11701 gaagtaggcg gagattctgc ccgggactac gaaaacagtt atgcaaatgc catattatgt
    11761 ttatgttata agaaacaagg taacgggaag actttataaa gggcaaacag aaaatcttga
    11821 aaagcggttg aaagcacatt cgcaatcaca aacaaagacc acaagttact ttccaaaaga
    11881 ttgggagttg gtttatcacg aaatttttag ttcaagaaaa gaagctgtag agagggaaaa
    11941 atattttaaa acagcagcag gcagaagata tttatctgga agaatagcaa atattaaatc
    12001 aacggatacc cgcccgaacg taccgttcgg tacgggcggg gaatagaagt ttcctaaact
    12061 tttgatggca gttcccagtc cgactacgga gtaggcggag attctgcccg ggactacgaa
    12121 gaaaaggcag ttccctgtcc gactaccaag taggcggaga ttctgccctg aataaaaaaa
    12181 cagttttaga aatgctatat attaaagatt aaattcattt tgtatggaaa actaaaaact
    12241 gatttcctat tcaataacca caattcaaca aacattcaca aactataaag gagaataatt
    12301 atctttgcgg aatagttata aatagatatt tttatgcata tccatttgtt tcgtgaacag
    12361 ttaaaacaaa aaggactaaa agttacacca cagcgtgtag caatttatga agctgttgca
    12421 gaactgcaca accatcctac tgcagagaat attattgagt acattaaaac aaatcatccg
    12481 aatatctctg taggtacagt atataaagtt cttgactcat tagtagagaa caacctgttg
    12541 cgaaaagtaa aaaacgaaaa agatattatg cgttatgatg cagttctgga gcatcatcat
    12601 catttgtatt gcgcagaatc cgatcgtata gaggattttg aagacccgca gttagatcat
    12661 ttcatcagtg aatattttaa gaagaagaag ataaaaaact tcaaagtgca agacattaaa
    12721 ttacagatta caggaacttt caaataaaaa aataaaacac acatattatg gacaacacaa
    12781 aaagcggcaa atgcccgttt catcatggag ccaacaccga agcaagtaca ggtgtcatga
    12841 actggtggcc caaagcattg aacttagata tcctgcatca gcatgataca aagactaatc
    12901 ctaatggaga aaaatttaat tacgcagaag aatttaaaaa attagattta gaagctttaa
    12961 aatctgacct gaaaaaattg atgactgaaa gtcaggaatg gtggcctgca gatcatggaa
    13021 gctattcggg tatgtttgcg cgtactgcct ggcacttagc aggcacttac agaaaagctg
    13081 atggtcgtgg tggtgcaaac accggcaatc agcgttttgc tcctctcaat tcatggcctg
    13141 acaatgtgaa tacagataag ggaagaagat tgctatggcc tatcaagaaa aaatatggta
    13201 acaaaatttc ctggggcgat cttattgttt tagcaggtaa tatggcttat gaagttgcag
    13261 gactaaaaac atttggtttt ggtggaggta gagaagatat atggcatcct gaaaaagata
    13321 tttactgggg atcagaaaaa caatggcttg caccaactgg tagtgaaggc acacgttatt
    13381 ccggtgaaag agacttagaa aatcctttgg cagctgtaat gatgggattg atttatgtta
    13441 accctgaagg tgttgatgga aaaccggatc cattaaaaac agcgcaagac attcgcgtta
    13501 ctttctcacg aatggcaatg agtgatgaag agactgttgc acttactgcc ggtggacata
    13561 ctgtaggcaa agcacatggt aacggaaaag tagaagtgtt aggtggtgtt cctgaagctg
    13621 cagaaataca acatcaggga ttgggttggt ttaatcctga tggctctggc aataaagata
    13681 aaactatggc aagtggtatt gaaggagcat ggactacaga cccaacaaag tgggataatg
    13741 gattttttga tttgttattg aactacgatt gggcattgac aaaaagccct gccggtgcat
    13801 ggcagtatga gccaacaaat atgcctgaag aaaagaaacc tgttgatgca tttgatccaa
    13861 aagttcgcag aaatcctatg atgactgatg ctgatatggc attaaagatg gatcctgaat
    13921 tcagaaagat atcagagaga ttttataatg acttcaatta tttttctgaa acatttgccc
    13981 gtgcttggtt taaacttacg catagagata tgggaccaaa gtcgcgttac cttggtgccg
    14041 atgtgcctaa agaagatttg atttggcaag atcctattcc tgcagttagt tacacattgt
    14101 cagatgctga agcagatgaa ataaaaacta aaattttaaa ctcaggttta agcagaacag
    14161 aattgattaa tactgcatgg gattctgcaa gaacatatcg tggatcagat ttcagaggcg
    14221 gtgccaatgg tgcaagaatt cgtcttgagc caatgaaaaa ttgggaaggc aatgagccga
    14281 aacgcttaca aaaagtgttg gataaactca cagaaattca aaaaggatta agcaagaaag
    14341 taagtattgc agatttaatt gtgttgggtg gtagtgctgc tgttgagcag gctgcaaaag
    14401 atgcaggcat gtcagtaaaa gttccttttg ctgccggcag aggtgatgca acattggagc
    14461 agactgatgt agattctttt tctgatttgg aacctttaca tgatggatac agaaattatc
    14521 agcaaaaaaa ctatgctgtt tcttcagagg aattgttgtt agacagaaca cagttaatgg
    14581 ggctcactgc acctgaaatg gttgtgcttg ttggaggaat gagagttctg ggaaccaact
    14641 tcggtggaag caaacatggt gtgtttacag agagagaagg tgttttaact aatgacttct
    14701 ttgtaaacct tactgatatg aaatatgagt ggaagcctgt ttctgaaaat ctgttcaata
    14761 ttgttgatag aaaaacagga gctacaaaat acacagctac acgagtagat ttggttttcg
    14821 gttccaattc aattttaagg tcatactcag aagtgtatgc acaggatgat aacaaagaaa
    14881 aatttgtgaa agattttgtg aaagcctggg taaaggtgat gaatgctgac agatatgatt
    14941 tgaaaaaata atattgttta gtactccaaa aatgcgagca gcctctgaaa agaggctgct
    15001 cgcattttat atcatctgct taactgtaac cactaaagtt ttgaagcaac cacaaaagct
    15061 tttttatact ttttagtttt tccttcggga ttaaatgctt cggcataaac aatataaatt
    15121 cctacagagg ctttatcatt tttatcggtt atgccatccc acataaaact atcttccggt
    15181 gctaatagtt ttgattttac caagttgcga accaattgtc ctgcttcatt ataaattttt
    15241 gcattcagca tataacccgg ttcattcatg ttgatgttaa atgttactac atcattatat
    15301 ccatcgttat ctggcgaaaa aatttctggg gttattgtaa aattattttc ctgtaattgt
    15361 tcgtttaaac tttgtgagtt tttatagccc ggtgttgcat agccaacagc ttctgctgcc
    15421 gaatgccagt tagtagaatc ctgtgtgcct ttattaaacc atattctttc taatgaaaca
    15481 ccattctgat cgtcaatgag tggaaaatgc caacgcttat tatagttaag ctgatcaatt
    15541 ctgttcaatg caaaatcggt aatgacacaa acacccatgt catcattaaa acccggcagt
    15601 gatgctttta gtaaattatt tgttgcagca ttgggataaa aactttgcag tgatgcaaca
    15661 tcagaagtaa gtgctatgta ttccttcgga aataaaagat accctccatc actaacacta
    15721 taatttgttg ttatcagatt gttgttatca gttgttgcaa ctttaagatt tttaaggtca
    15781 attatttttg atgagttgtt gtaaagctcc acaaagtcat ttcctcctgt ttttgggtta
    15841 aacaatattt cgtttatcag cacatcacct gcaacagcag tttctgcaat accaattttt
    15901 aaactaagtt ctcctgttat tttatttccc ggacagtcag caattgatga gtttaaatta
    15961 acatgataaa ctattccggt ttgcaatgca ttatttagcg ttaaaaaggc atgattgtct
    16021 gaaagtaata ctgatgcagg ctgacctatt ccattgtcaa taatataact tgatgtttgt
    16081 gatgcaatag cattgtcagg attctcgcta aatgttattc ttatcaatgt tgtggtaagg
    16141 ggataagcat caactgcctg tggtggggtg gtatcgctgt aagtaccttt aacactgttt
    16201 acttttcccg gtgttccgcc ttgcgcatca tttgatgcat gccagttaaa tcttgaatta
    16261 caagtaaaat cagcatcaat tctttcaatg gtgaagcctc ctgttttttt gctgtcatcg
    16321 ttgtagttat catcactgaa aattactctg tctatcaatg tgccattgtt atcagtcagt
    16381 ttaaggtcat caccaacatc attatttaac gaaggaaatg aacttgtccc tgttgtatta
    16441 ccatagggaa taaatgatgt tgtgtcagaa tttttacaga caatgatata tgcacttggt
    16501 tgaagtgtta ctgcgccaat acttgccacg gaggttgacc cgtcagataa tgtccagcca
    16561 ttgagtgaga gaattttgtt acttctgttg tacagctcaa caaactctgc attaggcagt
    16621 ggtgccgaca caataggttc aaaatatatt tcattaatga caacatcaaa aggctgtgct
    16681 gttacaggag cattccatgt aaagcctata gtgcttcctg cagcaattac attgctatta
    16741 agatcagata cattacttac agagagcgtg tatgcagtgc cacttgtaaa tggagtgcta
    16801 aatgtaagat gtactaatga aaaatcactt atatcgcgta ctgcagttgc cggagtgccc
    16861 aaaccctgaa ttgcataatt cgttgtagtc tgtgatgtag tcaggtcaac aagctcagaa
    16921 aattttacat ctaaagcagt attcgataac ggaatagcgg aagttagtgt tggtggtgtg
    16981 atgtctgcaa taaatggaat agagaaatcg tcaaagaaga agcctgtggc attggatgaa
    17041 gtgtaagtac aactcacacc aaaataactt cctgaattaa ttgttgcatc agtacctgaa
    17101 gattgtaaag taaaatttgt tcctgcagca gcatctgcat atatactcca taggcctgca
    17161 ttatctcttg taatttttac actgatagtt gatgccgctg caataaatcc attggtgcct
    17221 cgtgctactg atacagaggt tgtacctgtt tgtttaaaaa tttcgatggc atcgttgctg
    17281 cctgtttctc caaactgtac ataatagccg ttgagtgcac cttttaagtt ttgttgatcg
    17341 ctcaccaaat aaattcttgc ataattactt gacgatggcg caaagttgag ctttatataa
    17401 aattgccaag atgtattatc gagtgtaggc aggcttgttg ttaatgccag ataggaagtt
    17461 gctgcaccgg tgctgttgag ttgcagttgc tgtgatgcat tgactataaa ctgactgttg
    17521 tcgccactcc atgcaggatt agcagtaaaa tcaccatcag aaaagttatc tgttatttgt
    17581 gcaacaccaa aaaatggaat cattgcgaaa aaaagtagta atcgcttcat gttaaaataa
    17641 aatataaggc agtgaagata agcaaacctc attatttaaa ataaataaat tgaaacagca
    17701 ttaagtaatg tgttttttat tcatttttga ggaaggaata aaatattatg aaagtagctg
    17761 tagttggtgc cacaggcttg gttggaaaaa ccatgatgcg cattctggaa gaaagaaagt
    17821 ttccggtatc ggaatttata cctgttgcaa cggcaaattc tgccggtaaa aaagtaatgt
    17881 tttcgggaaa ggaatataca gtgataactt gtgaagaagc agttgctctg aaacctgata
    17941 ttgccttatt ctctgccgga ggatcaacat ctttagagtg ggctccaaga tttgctcagg
    18001 ttggaactac tgtaattgac aactcatcgg catggcggat gaatgaaaaa tgccctttag
    18061 tagttcctga aataaatgct gatgtattaa ctaaagaaga taaaattatt gccaacccaa
    18121 attgttctac tattcagatg gtgatggtac ttaatccgct acataaaaaa tataaactta
    18181 aaaggatagt tgtttctact tatcagtcag tttccggaac aggaaaaaaa gcagttgatc
    18241 agttgatgaa tgaacgcaaa ggaatttccg gtgctatggc ctatccatat aatattgaca
    18301 tgaacgtgtt gccacatgtt gattcctttt tagaaaatgg atatacgaag gaagaaatga
    18361 aaatggttaa tgaaacaaga aaaataatgc gctctgacaa aataaaagta acagcaacaa
    18421 ctgtgcgcgt accggttgtt ggaggccata gcgaagcggt gaatgcagag tttgaaaatc
    18481 agtttgaatt gagtgatgta agacaacttt tatcacatac tgcaggtgtg acggttattg
    18541 acgaccctgc acaacttcag tatccgatgc ccacttatgc agaaggaaaa gatgatgttt
    18601 ttgtaggaag aatcaggcgt gatgaatcac aaccacaaac attaaacctt tgggtggtat
    18661 ctgacaatct cagaaaaggc gctgctacca atgctgttca aattgcagag tatttgctaa
    18721 agcagttttg gcaacattaa agcttgtaag tgataaaaat tcaaattgtt tacaataaat
    18781 actttaccta tttgacagat tgaaatagaa aaaacttgat attgacagtt gttgacttaa
    18841 attacaattt gatttgaaca gtcttgaatt tatgttaaat taacgatccc ataaagttag
    18901 atagttataa gataaattaa gtaatgagat aacggactat tgctaaggga tatagtgatt
    18961 ttttaaaact caattattaa acagaaaaaa tagtatattt gaattcatga attggcagga
    19021 atacataatg tctgatgagg aagtactttt agggaaacca accataaaag gtacaagaat
    19081 ttcagttgaa catattgttg ggctattagc tcagggttgg acagaaaaag aaattttgga
    19141 aaattttcct cgattgacaa aagaatcttt gcaggcagtt tttttatata ttcaagactg
    19201 ttttagtgac ggattattat ttactcctaa aagaaaaacg gcttgaattt aatttatgaa
    19261 acttctagct aatgaaaatt ttccattaaa aagtattgtt taccttcaaa acggaggatt
    19321 tgatattact tccattgggg ctgaacactc aagcattaaa gattttgagg tgatgacaat
    19381 cgccattaat gaggagagga ctatattgac ttttgataga gattacggag aattgatttt
    19441 taaacacaat tacaaaccac ctaaaggtgt tatatattta aggttagaaa aatatagtgc
    19501 cgatgaaccc gggcgtctga ttgaagaatt ggtaagcagt aatcaatata tttttgatgg
    19561 tgcattaaca gtattggatg aacggggaat taggcagcgc aaatattagt agtttgttaa
    19621 attgtaatat ttttaaagta atttattctg cattgtatct tgtaaattac aaattggatt
    19681 catcaaagca tacaaaaaaa agccacccca aaaaggatgg cttttctttc ttaaaaatta
    19741 tttttttagt tgcgtcttcc gaagtaaatc aagacataat acatcagcgt tgctaaggca
    19801 cctaatgcag caacaacata ggtcatggca gcccaccaca aagcatcttt tgcttttgca
    19861 tgttcgctgc ttgttaccat gcggctgtcg ctaagccata ccaatgctct gcggcttgca
    19921 tcaaactcaa caggaagtgt aataaaacta aacaacactg tcatggcaaa caacacaata
    19981 cctgcaagta gtagcgatgg aaaaacattg atgagtaaaa caccgccaag caatacccac
    20041 tgtacccatt ttgaggcaaa acttactacc ggcactaaag ccgaacgaac ctgaagcatg
    20101 gtataagctt ttgcatgttg cacagcatga ccacattcgt gtgctgcaac ggcagcagca
    20161 gcagcattac gtccataata tacaggctga ctcagattaa cggttttgtt aagtggattg
    20221 tagtggtctg tcaactgacc ttcaacagaa ataactttta catcattaat accgttgtca
    20281 tgaagcattt tttctgcaat ctcctgacca ctaagacctg ttcctaaagt ttcatgcgag
    20341 tattgttcaa actttcgttt tagctgggcg cttactaacc acccgacaag cataaatact
    20401 attactataa gatatattcc catttattga tttttttaaa tttttaaact gcttttcact
    20461 aatttttttt aaccacaatc aaacagtgtg caaatataca caacctgcat ttttgtcagt
    20521 gttttttatg attattattg aatgaattac tcccagcgaa acgttttggc agctacaatg
    20581 taaataataa caccccaaag cagcaatacc attaactgtg aaatgacatc tattaatcct
    20641 gcaccttcaa atgccacttc gcgtaaagca tcattaaggt aggttaacgg cagtgccttg
    20701 caaaacggtt gcagccattt tggaaaaaca tcaatcggaa aaaatgttcc cgacaacaaa
    20761 aactgcggca tagtaatgat gttggcaatg ggtggaattg tgctttcact ttttgcaatt
    20821 ccggaaacca caaagccaaa acccataaaa acgataagtc caaaggcgca gataagcatc
    20881 attgtaagaa aagtgcttat gccattaatg agtgtaaacc caaatgcaaa cctgcccaca
    20941 cctattatga agcaagcacc aagcaatgaa aataccattc ggcttaatgt ttcacccaga
    21001 acaatgtaaa tacgttttac tggcgtggca aaaaaccgtt ttatgaccaa ggtttgtctg
    21061 aggctgaaaa aaacaaatgc agttccgaaa actcctgttg acaataaggc aaaacctaac
    21121 tgaccgggaa gtataaaatc tattttcttg tattcgcgtc ctgcaatttc ttcatttttt
    21181 aactgtgcca atggatgtgc tacatgtgca tcgtacaaat ttattttgct tataagatta
    21241 tctaaaatgt ttttagaaag tgctccacct tcaggtgcag ctgtggaggt aataagtctg
    21301 gccgtataat ctattttgcc ttcagtaata ttttttgtga taaccaaaat cccatcaatc
    21361 tttccttgct gaagtttttt acttgcaata gcagaatcgc cttcaatcca attgataacg
    21421 tcaatgtttt ttgtggcttc ataaatcgga ttatgatggt cgcttgaagg ggttagaaat
    21481 aatttgactt taagtcctgt acccgataaa aatccaaaaa ccaaaataaa ggtaagcgga
    21541 aaaaccagag taaaaactac tgcactgggg ctgcgtgtta ttgacctgaa actggcttta
    21601 gcaatagcca gcatggcctt tgtttgagaa taactcatgt tgtttatgtc agaaatgttg
    21661 caaatgtagt taagttggtt tttatgaatt aaaataaaaa atgtacgttt gccgtaataa
    21721 cataggttcg tcaattcaat gaatcacaca ttctcattat ttcagagcat ggagagggat
    21781 aagattatcc tttctttcaa aggagatatt acccaggatt tgcttgcttc tgtatatcag
    21841 attatggagt cgagaatgga tcaggataag actgattcca agaggaagaa aagattttat
    21901 cacattttag ttgaatgtct tcagaatgta tatcaccaca tggaagattt cagaaatgtg
    21961 catgagctgg atgaaatttc tgatggctct gcaattttta tcattgccaa ggcaaaaggt
    22021 gagggctaca gaattattac aggaaactat atcaagaaag atgaatcgga agaccttaaa
    22081 tcgaagcttg agaaaattaa ttctatgaac accgatgaac taagaactta ttatcttgaa
    22141 acactaagca ctacagagct ttcgcaaaaa ggtggtgccg gtttaggaat gattgatatt
    22201 gcccgcaagt cgggacataa aatagtttac agttttgacg gtgtaagcga cactgttaat
    22261 ttttttaccc ttgaagtaac aattaattaa atcaaagaaa atggataagc tgattattga
    22321 accgggcata aaaacacctc acattgtgtt tgacgctgcc aatggaagtc ttgaaattaa
    22381 aggtaaatcc attccggaaa attctattga attttataag cctgtttacg attggcttga
    22441 tacttataca acacaaccca aagatcaaac cttgctgaca gtgcagttag aatattttaa
    22501 cactagctct tctaaatgtt tgctcgactt attcaggaaa cttgaaaaca ttcattcttc
    22561 aggcaaagga aaagtaaatg taaagtggct ttatgaggaa gatgatgaag acattatgga
    22621 agccggtgat gactatcaga ccattgtaaa acttcctttc agtatcgaga aaatttcagg
    22681 ataattattt tccttcaaaa atttcgtcaa tagtttttac agcaactgca tgataacccg
    22741 gacgtgccga cttataaatt tgttctatcc aaggtcgtaa tgttgcatct gctgcagctt
    22801 ttcgataaag tggtgcaata aattttctgc gtcctactgt attcagaaaa ttttctaatg
    22861 ctttatatgc aggcttgtag tgacaggcaa aggctatcat aaaccaatca aatgcaattt
    22921 ctgaattttg aatctgagtg aaatgaaaat gattatctaa tgcaacagct ttttcgtagg
    22981 tcatttcggg agcaatagtt cgtaaaaaat gcaggtaatg atgcgttgtc catcctgcag
    23041 tatctggcat aactgttggc tgtttgataa atgctgctgc tgctttctca acctgatcaa
    23101 aatatttgct tttaataaca ggcagactct taggaagtcc ggttccataa acccattcat
    23161 caacatttac tttttgtttt atcaacggat tttcctgcaa aagatttacc tccagataat
    23221 gtagaaattc ttttgttgtc atggtttgaa agcgatgttc ttcaaaatac ttttttaaaa
    23281 atgcatccca catatcacgg ccggctgctt cttctaatgc catcagaaaa aaacgccctt
    23341 tttcataggc aatatcaccc atgccatcat caggatcacg acctttaagg tcaagaaaaa
    23401 gtttagtatc cggattgtta acacccatat cggcaacggt atgtttcaat tcattttgtg
    23461 aaagtaagtt cagcatgtca gcataatctt taccataaat tttttccata atgcgcgatt
    23521 caaagtaaac tgtaaatcct tcatttagcc agaagtcatt ccatgttgca ttggtaacta
    23581 aattgccact ccacgaatgt gcaagctcat gtgcaattag actcactaac gaacggtcgc
    23641 ctgtaatgat ggtaggtgtt gcaaatgtta aacatggatt ttccattcca ccaaaaggaa
    23701 aagaaggagg cagcaccaaa acatcatatt gtagccatgg gtatttacca tacaattgtt
    23761 ctgccgaaga aatcatagca ggcaaatctg taaactcatg aaaagctttg gccaacataa
    23821 caggttctgc gtaaatgccg gaacggttgt cgtaggcttt gtaagcaagg tcgccaatgc
    23881 ttaaagccat aagataggaa ggtacagcaa aaggcatttt aaatgaatag gtgccattgc
    23941 caatttgttt aggattttgt ggacaactca tcaatgccat aagttcttta ggacaatgaa
    24001 tggttgctgt ccatgtaaat tttattccag gtgtatcctg caaaggtatc cagctccgag
    24061 ccagaatagc ttccgattgt gtatataaaa aaggatgttt tttgcctgca gtttgttccg
    24121 ggtttagcca ctgtaatgct tgtgcatcag gagaagtttc ataataaatg gtaacctctt
    24181 ttgtatttgc tgttatagga atagcaagtt cgctgcctaa aatttcatct gcaggtttta
    24241 aaataaatgt aacaggagca tcattgattt ttacactttc aattttcagg ttgtaggtgt
    24301 ctaaacgcaa agtatcggca ttggtaagat tttttacttg caaagtcaca aagccaacta
    24361 actttttagt tgagaaatca gctttaaggc ttaagtcaag atgctgtaca acaatttctg
    24421 ctggattgga atatgaatgt tgatctttaa cgtatatccc tctttcatct tccggttttt
    24481 tagcaaacat ggtaaatggt attagaatta aaaataaaat atttttcatt gttcaattat
    24541 tttgttttaa tcatataaat atgaggaatg ttatcttcca gaaattcttc gctgatgaca
    24601 acaaacccaa atgactcata aaatttacga agataacttt gtgccataat ttttattgca
    24661 ctattaggga ataagctttt acattcatct aaagattttt cgaccactaa ttttcccaat
    24721 cctgttcgtc tgagttgttg atgtgtaacg atgcgcccaa cagaaacttc atcatacaaa
    24781 acaccgggag gaataatacg tgcataacca acaacttgtt gatttgccat agccatcaga
    24841 tgaaaagaaa gtttatcggt tttgtcaatg tcaccataag gacaattttg ctccacaata
    24901 aaaacctgct gcctgagttg taaaaaatca tgcagttcat gtgcagtgag gtcattaaac
    24961 gatttaaact tccaaaaaat attcatgctg tgatgggctg atatggcagc gaatataaaa
    25021 cacattttat gaatagtaaa agaagtaata cgattgaata aattgttagc agcattttga
    25081 tgattaacac tatttacatc aaactaaata aataactttg gcgccatgtc attgccacaa
    25141 ctcattgtta ctgacagcag ccgaaacgga aaacttccat ttgctccttt taaaaggaca
    25201 caggaagaat ctgcacaaca gccaaagaca gagaaggtaa gcaacaaagt tgcgaagcag
    25261 aaactttctt taaaaacaga cagcttaaaa ggtaatacca ctgtagttga tacaataaaa
    25321 ccaatagcaa acgatagtat tcaacttgca attgattctt cagttgcaga tatgtctaga
    25381 ttgtttcgtt ttacagatga ggaaaatgat gaactgaaca attcagatca acaatttatt
    25441 tcatcatcgg catatttcaa acagcagcag ttggaagtaa aatcgtcatc tccacaattg
    25501 tttaataaga tagttccgga ttggtttacc atagtccttt tttttctgat tgtgggcatt
    25561 acaacagtaa aagtttttta tcagaatatt ttcagacaat tggtcgaagc tgtttatagt
    25621 tttgcagtaa ccaatcaggt ggtgcgcgat gaaaatttgt tagtgcaacg tgcttcagtt
    25681 ttgctaaatg taatttttta ttgtagctca ggtttgtttt tttattggat cagtgtgcgc
    25741 tacaactgga atcatccgat cttaaatgaa ggattgtttc gttttttctt ttttgctttc
    25801 gtgacagcag ttttttttac agtaaaaatg ttgttgctga aactgatagg cacaattttt
    25861 aatctcgata aaccggtggc agtttttatt ttttccattt ttttgaccaa taatttacta
    25921 ggagttctgc tggtgttgat tacttcattt atggcatatt tatccggtgt gggtctgata
    25981 aattacatct atctggtatt aggaattctt tctctggcat ttattttttt attgtacagg
    26041 gcatttttga tttcccgtac tgtgcagggt tattctttgt attatttaat tttatacttt
    26101 tgcacccttg aaattgtacc gattgcattg atagtaaaat ttgcatcgga gtaattaaac
    26161 aaggtgatta aaaacgcatg gccggaaatt cgaaagtaaa gacaatactc gtaacacaac
    26221 ccaagccgga aacggaaaag aatccatact ttgaccttgc aaaaaagttc aatgtaaaaa
    26281 ttgattttcg tccttttatt cagattgaag gaatacctgc aaaagatttt cgcaaagaca
    26341 gagttaatgt tgcagaattt caggcagtaa ttttaaccag tcgccaggct gccgatcatt
    26401 atttcagaat ggctgccgaa atgcgcatga caaattttga tgaagtaaaa tttttctgcg
    26461 tgtctgagtc aactgctttt tatctccaga aatatatcac atatcgtaag cgcaaagttt
    26521 tttatggcaa caacagcatt aacgatttga cagatgtgtt gaagaaacat aagaaagaaa
    26581 agtttttgct tccttgtagt gaagtaatga atcccgacat cattaacaca ttaaaagacg
    26641 gtgacctgaa ttttataact gcagcaatgt acagaacggt aagtagcgat ttatctgatg
    26701 tgaatattga aacgtttgat atgattgtgt tcttcagccc atcgggagtt cagtcgctgt
    26761 tgaagaattt tcctaaatac aaacaaggca atacccgaat tgcaacattc ggtgtcacca
    26821 catcacagac tgcaaaagat gctaagttaa aagtagaggt tgaagcacct gttccgcagg
    26881 caccaagtat gacaatggct attgagcagt atctgaaaaa agccaacaag taatttttta
    26941 cttaatctca cttagttttt gctgtgcctg tgtagcgtaa aatcctttgc tggaaattat
    27001 tttttgtaat aactcttttg ctttatcctg ctcacctttt tgcaaaagca gcaatgctct
    27061 gtgccacgct gattcctgat taaatgtatt gtttgactgt gcatctaatc ggtcgaggaa
    27121 gtgaagtgct ttatcatact gctgactttc gctgtatgac attgccgtat aaaacaaggc
    27181 atttacatcg ctatgattgt cggatagcaa aagttgcaac ttattaattg cagaggagta
    27241 tttcttattg ttgtaatcaa ctaatgcttc agctaaaaaa tcttgatatc ctattgtacg
    27301 agactgcatg ttgttaagcg ttttggattt ttccatatcc tttctcgact cataacgggc
    27361 aggaatgccg ttgtcttctt ttgctacgtt cttttcacgt ttgtactcac gggaatagtc
    27421 atacactttt agtccttcaa tattttttgt taccggcact acagcatcaa ctacagcagc
    27481 acctgccgat gcagactcag taactattgg tgcagcaaca tctgctgact gtggctcagc
    27541 aattgcttca tttttctgaa caggttctgt tgcagcaaag gctttatctt ctttacttgc
    27601 ttttgctacc attgcattat gcgatgcaat tatgtttttt gatgcttgtt gaattctttc
    27661 ttctgcagga ggaggtggtg gttgaatggt agattcattc aacttagtat cactgctggt
    27721 ttgaggtgtt tctgtattta ttttttgttg tacagcaggt gcgttaattt gttctgctgt
    27781 gttttcatgt ttgaaggaat agttagaaat aatccatcct gtagcaatta aaattgcaac
    27841 agatgctgcg gcataatacc atctgtttat agatggggat tgttttgcag aagaagaata
    27901 tttatccgat acctgcttga tggtttcatc aagcggtttc atagaggtca acaaggcaag
    27961 accttctgct gcatcggaac aaagctcgca gtctgtcagg tgtttttcaa cggcatactt
    28021 ttcggcatcc gtcatttttc cctgcacata ttttgtcaga cgttctttgt caacgcagtc
    28081 agaggttttg aatatttcaa ttttacgact taacatgatt atttttctcc atataaattc
    28141 tgagattcct tttcccattc tgtataaaac tcttcacctg cttgaaatca aaacctgtta
    28201 ggtctgttat ttcattatag catttttctt tcaggaaaaa caattcaata catattcttt
    28261 gttcttcgtt taatgttttt attgcttctt caagcatcag caattctgtt tctttttcat
    28321 aaacacggtc ggtgttttcc ggttcaaatt ctgttgatgc agcaatttca tctcggctgt
    28381 aattgtctgc tctgtttttt gctgcctgtc tgctgcgcaa ctgcatcaga caaaagtttt
    28441 gtgccacacg atgtatccag tacgagaact tctgtacgtc atgttttgac aaatcggcat
    28501 aaagtttctc aaaaatctgc aacacagcat ctttactgtc gtcagtatct ttaagatact
    28561 tcatgcacac accaaatact aaatgcgaat agcggttaaa caatataccc acagcttgtt
    28621 tatcaccttg ctggcgatat tgcaacacaa gctcctcatc gctaagcgca ttcttatcct
    28681 tattccggat gagccacata aataattttt actttctgat taatggtagg atgctttctg
    28741 gaatatgatt ccataaacat tcataaaaat aattttaaaa ttaaaggact acctccgcaa
    28801 tggcatgaat aggtatatct tttcctgcct ttaattccac atggtcattg gtgacagccc
    28861 aaacagttgt ttctacagca caagtgcctt cagtggtttc aaatactatg cgcacttttt
    28921 gtttgtaatc gtttcctaat gccattgcac catcgagtaa aatttttcgc cttgcacgat
    28981 ctatttcatt cgataacact tcgtgttgaa caaaatggta gtcagataaa tcttctttag
    29041 caatgatggt gagttccata ttgattgaat ttggtgtatc agagttttag cggcatataa
    29101 aagtaggcat ttattctaat atgttcaata aaattttaaa acggccattt ttcccgactt
    29161 tccggccatc cagacctttt tttgtgaagg tgctatgctg atggtgtaaa atgctagtga
    29221 tagtccatct gcgtttttaa tttctttaat gtagtgctga ctgtcaatca catacgtgcc
    29281 gttatggcct gttgtaatca ggtagttgcc atttttagtt tttattgttt taacacatga
    29341 cacaaaagcc ccctcaggaa gttttatggg ttgccatgtc ttgccaccat tgtttgtttg
    29401 gtacaaagtt gtggtagtat cggattgcaa atagttgcct ccaccaatat agccatttag
    29461 tttgttttca aaatacatag taaaaattcc tgtcatggtg ctgtcgctaa tcataggtgt
    29521 ggtttgtgga agaaatgcac catcattttc agaaataaaa agtcttgatg tgcttcctcc
    29581 tgttgcaaaa atcacgctgt tttgcagcca ttgtatgttg gtgttacttg cagcaaagca
    29641 accttcacct tttattgcag gtgatgtaac ttttagctcc tgccagttgt tgccgccatc
    29701 tgttgtttta ataatcataa aatacccttc cattggatcg cttatagcaa tgccatgaag
    29761 cgaatcttta aatgtcatac tatcaaaaaa aatacccttg ctgtgattgc tgtaaacatg
    29821 ttgccatgat gtaccataat tatttgttct caatagatat gccggtgaat caatgctcaa
    29881 taacaaaacc gttgagtcgt ttaatgccgc caatgacctg aattcagaat attttccatc
    29941 agtggcaata gagtctatat gccagttgct gccaccatct tttgtaaaac caaaaacacc
    30001 tctgttagct gcaaaccaca ccacactgtc gtttactgtc tgtagtgcgc gaatgcttac
    30061 ctgaacgggc aattgaatgg tttttaattc tatctgctgt gcctgcagtg atgatgtaaa
    30121 acaaaagcac cacagtatgt gaaaaattct ttgcattgtg tttcaaatgt agtgtttttt
    30181 caagtctgca tagattgtta aacgaacaga ttgtaatttt tttaagacta ataggtagtg
    30241 acttaaaaag aaagatatgt tttcagaaaa aagtaatgac agaaaggttt atattgtagc
    30301 catcagaatg tttgtattat aatcatgtca gcgcaactta accgtgtcat agcacacatg
    30361 gacctcgatt gtttttttgt atccgtatcg cggctgctgc atccggagct aaacaataag
    30421 ccggtaattg ttggcggtgg cgagcgtggt gtagtggctg catgcagcta cgaaaccaga
    30481 aaatttggtg tgcactctgc catgcccatt aagcaggcaa aacgcctgtg cccggaagct
    30541 attattgttc gtggcgatta tgatgcttac agcagtcatt ccgatatggt gcatgaaatt
    30601 atttgcgaaa gtgtgccgct gtatgagcgt agcagtattg atgaatttta tttagaccta
    30661 acaggcatgg atcgtttttt tggatgttat aaacatgcta cagaactcag gcaacgcatc
    30721 atccgcgaaa cagggctgcc tatttctttt ggtatgagtg ccaacaaaac cgtaagcaaa
    30781 attgctaccg atgaggcaaa gcccaacaat cagatgcagg tggactgtgg cgaagagaaa
    30841 atttttttag ctccgctgtc ggtacgaaaa attcctatgg taggcgaaaa gacctacaca
    30901 ctgctgcgca gcatgggtgt agagaaagta aaaaccgttc aggaaatgcc tgtagaactg
    30961 atggagcgtg tgcttggcga aaacggaact accatctgga aaaaagccaa cggcattgac
    31021 accacagccg ttgagccata ccatgagcag aagagcatga gcaccgaaga aacttttgag
    31081 caggacacta ttgacgtgca acgcctgcag cacatcctca ttgctatgac agaaaaacta
    31141 tgcttccgcc tgcgcagcga acacaaggta acgtcatgta taagcgtaaa aatccgttac
    31201 agcaacttcg acacacatac catgcagtgc cgcatagcct acacaagttg cgaccacatt
    31261 attattaagc aagtaaaaga actgtttgct aagctgttta accgcagaat gctcatcagg
    31321 cttattggta ttaagttcag ccatctggta ggtggcggac atcagattaa tttgtttgaa
    31381 gacagcgaag aaatcatcaa cctctatcag gcaatggacc gtatgcgcct gcgctttggc
    31441 gaagataaaa ttcaccgtgc tgctgccatg agcttttcat tgcgcggatt caatccgttt
    31501 aatggattga gtaattcacc taaagagata aagggcggta aataagctaa aaaaatagtt
    31561 gtagaacgat aataccttga aaaggcaaaa ttccatattt acttttacgg tcttaatttt
    31621 aattatatat attcataatt aaacttaggt acctactatg gagtttgata aaaacatttc
    31681 tgattaaaca acacaaaaca atcaaccttt caaagatatc ttactgtatt tgttcctata
    31741 ttccagcgga gtgatgcctg tatgtttttt aaataaatct ctgaatgctt ttgtgtcagt
    31801 ataacccaca tcaaacatta cttcagtaat attttttctg ctgctttcaa acttgcgttt
    31861 tgcagtttca atttttactc gttgaatata ttccaatacc gaatgattgg ttgacttcag
    31921 aaatcttcgc tcaaaagttc ttcggctcat ctttgtatag tctgcaagtt ggtctatagt
    31981 tagttttgca tcagtgtgtt tttctatata ctgttgtgct tttaaaattt ctgtgtcatg
    32041 atgatttttc tgaccattga atatggcaaa ggaagcctga ctgtttcgat ccaaatcaat
    32101 agcaaagtat tttgaagcaa gaatagcagt gtgtcggtct gaatattttt ccaacaaata
    32161 aaacagcaga ttccacagcg agtttgcacc accgctgctg taaatgccac gctcttcggt
    32221 aatgattgcg ccatcaacta cctctgtatt tgcaaactgt tctttaaact cattaaaata
    32281 agcccaatgt gttgacactt ttttgccgtc aaccaaacct gatgctgtta gtagaaaagc
    32341 accaatacat aaacttgcaa cttccgtacc ctgacgatgc attttttgta gccatggaat
    32401 ggcagctgca tttttcttta aggaatgttt tacatcacca taaagcgcgg gtataattat
    32461 taagtcagaa tcgttaacac tatttatatg cctatcaata cgaatgctgt attcgccatc
    32521 attggctttg atgctttttt gtaagcccac atattcaact ttaaacttag gttttttacc
    32581 tgaggctata agaatatcat tggcagtttt aaataatctg tatgccggtg ttatggcttc
    32641 tatagctgca tgatacggta cataaacaga aattaacatt ccggtaattt ttatttggta
    32701 aagttaaagt attttgtcat tatcacacct taaaactgtc gtttctgcca cctattgttg
    32761 ctttctttaa tactcatctt tgtagtacta aatcaattat tcactattaa gcaaaaaatt
    32821 atgcaaacaa ataatgtaaa tctacacagg gtatttgctt cgcctacaga gaaagtgttt
    32881 aaagcatttt ctgatgccga tgcttatact gcatggctac ctccttatgg atttgtggcc
    32941 aaaacacatc aatatgattt taaagttggt ggcagttata aaatatcgtt tattaatttt
    33001 acaacaggct ttagtcactc tttcggtggt gagtttgtag agataaagct taatgacctg
    33061 ataaaaataa cggatcgctt tgatgaccct aatcttcccg gagaaatgat aactactgtc
    33121 cattttaaaa aagagagctg tggtacagaa attaatattt cgcaaacagg aattcctgtt
    33181 gccattcctt cagagatgtg ttatttgggt tggcaagatt cattagataa attaaaaaga
    33241 ttggtcgaac ccaatatacc ggatgcttag tttattcgct attaaattta aacaaacata
    33301 aatcaatata ataacaattt tcaaaacact atcattatgt caaaacaagt ttttattaat
    33361 cttcccgtaa aggatttaaa gaggtcaatg tctttttatg aagctatagg cttcaccaac
    33421 aatcctcagt ttacagatca tactgctgcc tgtatggttt ggagtgacac aatttttgtc
    33481 atgcttctaa ctcatgataa gtttaagcag tttacatcca aactaattat tgatgcaaaa
    33541 cagtcagtag gcgtttctaa tgcacttttt tttgaaagta ttgatgctat gaagtcgttt
    33601 gcagaaaatg cagtaaaggc cggaggcaga gaatatgcag ctgccagcga ctatggtttt
    33661 atgcaacaac attttattga agatttagat ggacacaatt gggagccatt ctatatggat
    33721 atgagtaaat ttcccggata aataacaaac aaattaaaat tatggcaaag aaaaaaatag
    33781 ctccatgctt atggattgaa aaagatgcta aaaaggcagc taaatattat ctctcaattt
    33841 ttaaagaagg caaaatgctt tcgtataaag catataaaaa ctcaggcaca cccagtggcg
    33901 attttgatat tgcatacatc aactttctgg gaatggatgt gcagattctt gctgcaggac
    33961 cattattcaa atttaatgaa gcagtatctt ttgttatcat ctgtaaagac caaaaagaaa
    34021 ttgactatta ttggaaggca ttgacaaaga atggtggcag cgaaggtccg tgcggatggt
    34081 gtaaagataa gtatggtctg tcatggcaag ttgctcccga gctatatttt aaattagaac
    34141 atcataaaaa taaagcaaag caggaatatg ctttcaaagc attgatgaaa atgaaaaaaa
    34201 ttatcattgc cgacttagta gaaaaaaagt aattgttggc aaaacgataa aaaacaaaga
    34261 agatgaataa aattactgtg agtgctatta taaatgcacc aataaaaaaa gtgtgggact
    34321 gttatacaaa ccccaatcat attgttaact ggaattttgc tgaccctagc tggcattgcc
    34381 cttctgctac taacgatatg cgtattggcg gaacctacaa agcaagaatg gaagcaaagg
    34441 atggcagctt tggtttcgac tttgaagccg tttatactca gattgaatcc gagaaatatt
    34501 tttcttatga gtttggagga agagttgctt cagttgattt caagaataac gatagccaaa
    34561 cagaagttgt tgttgtgttt gatgccgaaa gtgaaaacct tgttgagatg caaagagcag
    34621 gatggcaggc tattctgaat aattttaaaa attacaccga gcataattag tatatgcaat
    34681 tatgtgcgta tctccatttt aatggtgatt gcagaaaagc aatgacattt tataaaaagt
    34741 gcttaggtgg aagactgagt tttcaaatgg ttagcgaatt gccgcagtca gaatcacttc
    34801 ctacttatat aaaaaggaac attctgactg ctttattaca gactgaatat tttcagttag
    34861 ccggaacaga cctgggtaat gaagcagttt attcgcctga caagagcttt tcgttactct
    34921 tagaatgcag gagcaaaccg gagttgaagc gattgtataa gcaactttct gcaggtggag
    34981 tgaagacaca attgcttcag aagaattttt atggtgtaat aattggctct tttaaagata
    35041 aatttggcat taactgggtc cttcagtatt aattgcacta ttttttaaat ttattttctt
    35101 tcagaagttt tgttacagtt tttcttaatg tttatattac aaccgaccga atgattgtag
    35161 tataaacata ttaaatgtgt agagtgacag ctatttggtt gttttaacat attgccatgc
    35221 acctctgttg ccactcatac ttctctctaa aatacggtgt ccttagtccg gacgatttat
    35281 tgtgcattgc cgcacaaaaa aatattcagt cgctggcact cacagatatc aacaacacat
    35341 caggcatact tgattttttt cgtttagcac agcgatacaa tgttcgccct gtggcaggca
    35401 ttgatttcag aaatggtgta gaacaaaaat ttattggtat tgcaaaaagc aacgaaggct
    35461 ttcatgagct caataaattt ctatcggagc atttaatttc cggagaaaaa ttttcagaca
    35521 ttgctcctgc attccaacag gcatggatag tttatccaat gttgtcggca ccacaacagt
    35581 tgcacgaaaa cgaatttgta ggcatcaaac ccaatgagct ctttcagtta aacttttcgg
    35641 agtggaaaag caagttacat aaattggttg ctcttgcacc ggcaactttt cgcaacaaaa
    35701 cagattttaa ttcacaccgt ctgctgcgtg ccatcagcta caatacgcta ctcagcaagc
    35761 tgcccgtttc cggacaggca tcggcagatg aaatcatgta ttcaccggag gagttgaagt
    35821 gcatttacag agaatatcca caactgattt acaacacaga gaaattattg gaaccctgtt
    35881 gtatagattt tgaattcgga aaaaacaaaa acaaaaaata ttttttgggt agtgccaaac
    35941 gcgatcatca caaactgatg agtctttgtt atgaaggttt aagttatcgt tacccgaata
    36001 ctgacgacac catcattgac cgttttgaaa aagaaattca gatgataggt gagttgggtt
    36061 tctcatccta ttttctcatc aatcacgata ttgttcgcta tgcacgaagc aagaattatt
    36121 tttatgtagg ccgtggcagc ggtgccaaca gcatggtagc ctatctgctg cgcattaccg
    36181 atgttgaccc tatagacctc gacttgtatt ttgaacgatt tatcaatccg cagcgcacca
    36241 gccctcccga ttttgatatt gatttcagtt ggaacgagcg cgatgatgtg attgactaca
    36301 tctttaaccg cagcaccttt ggtaaagaac atgtggcact gctggcaacg tatagcacat
    36361 ttcaaagaga tgctgccatt cgcgagttgg gaaaagtttt tggattgccc aaggcagaga
    36421 tagattcttt ttttgaaaat ggaaatacca aacgcgacca catcagtaat ttggtgttgc
    36481 aatatgcaca acgtatcatt gatgtgcctt cgcacctgag cattcatgca ggaggcattc
    36541 tcatttccga aaaacccatg acgtattata cggctctcga taatccgcct aaaggttttc
    36601 cgcttacgca gttcagtatg ctcgaagcag aagatgtagg tctttataag tttgatattt
    36661 tgtcgcagcg cggactggga aaaattaaag atgctgttgc tgtcattgaa aaaaaccgac
    36721 aggtaaaagt tgatattcat gacataaaaa agtttaaaga agatgagggc gtcaggcaaa
    36781 aactgcagca ggcaaatctt atgggatgct tttatgtaga aagtcctgcc atgcgcatgt
    36841 tactgaaaaa actacgtgca aaaacctatc tcgatttggt ggcagcaagt tcaatcatcc
    36901 gtccgggggt ggcacaaagc ggcatgatgc gcgaatatat tttgcgcttt catgaggaga
    36961 gccgcagaac ctacattcat ccgatgatgg aggagttgat gcgcgaaact tttggggtga
    37021 tggtatatca ggaagatgtg attaaggtag cacattattt tgccggactt acactcacag
    37081 aagccgacat gctgcgcaga ggcatgagtg gaaaatatcg gggacgtgca gagtttgaaa
    37141 aagtgaaaga tgcttttttc aatggttgcc gcaagaaagg acatgccgat aagatagcag
    37201 aagaagtgtg gcggcagatt gaatcgtttg caggctatgc tttcagcaaa ggccattcgg
    37261 catcgtatgc cgttgagagc tatcagtgca tgtttttaaa gacctatttt ccacttgaat
    37321 atatggtggc atgtgtaaac aacggtggtg ggttttacgc agctgaattt tatgtacatg
    37381 aagcgcgtat gtgtggaggc accatacatg cacctgacgt taataagagt ttttatgaaa
    37441 catcgctcta tgggaaaaat atttatgcag gctttcattt agtgcatgaa cttgaaatgt
    37501 tgacggtaac aaatatttta aaagccagag cagcagaagg gcagtttatt tcgcttgcag
    37561 attttatgca gcgcgttgaa atttctctcg aacaattgcg gctgctcatt cgtgttaatg
    37621 ctttgcgttt tacaggaaga accaagcaac aactgctgtg ggatattcat gaaataatgg
    37681 ggcatgataa aaaaacaaat acagaaaaac agttgtttga agccgaacac aaacattttg
    37741 tccttccgga attgcattac agcaaaattg atgatgtgtg ggacgaaata gaattgttgg
    37801 gatttccgct gtgctcacca tttgatttga taaaacaaaa aacgaatgta acggaaggaa
    37861 tagtgattca tactgctgca gaactgtcgg catactgcgg aaaaataatt tccattgccg
    37921 gttattttgt tacccgcaag ccaacacgaa ccaagcatgg gcagcctatg gcattcggta
    37981 cttttctcga cagcaaagga caatggatag acactacaca ttttcccgat gtggtaaaac
    38041 aatatccttt tcgcggacgc ggatgctacc gcattaccgg aaaggtagtg caggaatttg
    38101 gtttttacag cattgatgtc atcagccttt atctgcttga atataaaacc agatatgctg
    38161 tggtggagga ggataaagtt catgtaaatg aataggagta aacaataaaa aaagtaaaac
    38221 ttccctaagt aggcgcaagc gtctttcgct cgtgctttta tccttgtggc ctctatccgt
    38281 ccgatagcta tcggacttca tagttcaacc agctttaaat cggtaaattg attagcacta
    38341 ctatactcca tacccgcgaa gttgcacttc gtgggttttt gtgcaaagaa gttttacttc
    38401 tgaaaccata ccgtttcctt tattttttat tgtgtaaata taatttaatg ttcactttgt
    38461 aaagcacagc attatgacac actactcacg aagtgcaact tcgcaggtac ggggggataa
    38521 agttcatgta aatgaatagg agtaaacaat aaaaaagtaa aaagaaaagt attacaatgc
    38581 atttaaaatg ctctacatgt gctaatattc tgaaacagag cacttaatta attaactatt
    38641 ttgtggtcag acctagacaa agaaataggt agtgtattga aaaacaacac tgtaggttag
    38701 tgcttaggtt tgttaaaaac ctttatattt gccgctctta aaaaaatacc gaaaaataaa
    38761 ttattcatgc aaagtaaagg cgcaattaaa tttttagcca ttctggtatc catcatctgt
    38821 ctgtttcagt tgtcgtttac catcgtaaca aatcgtattg aaagcaaggc acatgaggca
    38881 actaatggca accccgaagc atacaaagca tacattgact ctatcagtca gttaacggct
    38941 tataatcttg gctttaagaa atacacctat caggaatgta aggaacgtga aatcaacctg
    39001 ggtcttgacc taaaaggtgg tatgaacgta acgatggaag ttagcgttac cgatttgatt
    39061 cgtaacatgg ccaacaatcc taacgatcca atgttagtga aagctttaga cgatgcacat
    39121 caggcacaac gtacaagcca gaaaggttta gtacagttgt ttggtgaagc ctttgaacgt
    39181 aacaatccgg gagcaaaact ggctgcactt tacagcacca tggacttgca gaacagagta
    39241 aatttcaact ctaccaatgc cgaagtaatt aaggttatac agtcagaagc agatgcagct
    39301 atcagccgca catacaacat tcttcgtaca cgtattgata aattcggtgt aacacaacca
    39361 aacattcagc aattaggcgg aggacgtatt ttaattgagc ttcccggagt aaaagagccg
    39421 gatcgtgtgc gtaaactgtt gcaaggaact gcacaattag agttctggga aacgtatgat
    39481 aatgctgaag tgtttccgca attagaagca gtaaataaaa aacttgctgc acttgaaaca
    39541 cctgcaccaa cagactctac tgcaaaatca gacacaacag ttgcagctac cggtgattct
    39601 ttaactgctg ctgcagatac aggagcaatg aaaaagccgg cattactcag caagttagag
    39661 agtgatactg ctgctgcaaa agacacttct tccagtgctc agtttgcaaa attttctaaa
    39721 gaaaatccac tctttgcatt gctgacacct gcgatcttta ccaatgcaca aaatcagagc
    39781 gaatatcgca aaggccctgt agtaggttat gctgctatta aagatacagg taagctgcgt
    39841 gaaattttag agcgtcctga aataaaaaat attcttccac gcgatttgcg tttgttatgg
    39901 acagcaaaac caccaacaaa agaatctaaa tttttagagt tagtagctat taaagtaaaa
    39961 agccgcgatg gaaaagcacc aatggatggt tctgtaatta cagatgcacg tcaggacttt
    40021 ggtcagttta acagctcgcc agaaatttct atgtcaatga atcctgaagg agctaaaaca
    40081 tggaaaagac ttacaggaga aaacgtagga aaaagtattg ctattgttct cgataattat
    40141 gtttattctt tccctaatgt acagggcgaa attgcaggag gccgttcatc cattacaggt
    40201 aacttcgaaa tttctgaagc acaggatttg gctaacattc ttaaggcagg taaacttcct
    40261 gctcctgcac gaattgttga agaagctatt gttggtcctt cattaggtga agaagcaatt
    40321 caaaacagtt ggatgtcgtt tgcaggtgca tttgttgtaa tcctgttgtt catgggattc
    40381 tattatatgc gtgcaggttg ggtagctaac atcgcattgt ttgccaacat attctttatc
    40441 atgggtgtgc ttgcatcttt aggtgctgta cttacgcttc cgggtattgc aggtatcgtc
    40501 ctcaccatag gtctttctgt ggatgccaac atccttattt tcgagcgtgt gagagaagag
    40561 ttaaaagcag gcaagggaat aagacttgct gtttctgatg ggttcaagca tgccatgcct
    40621 tctatccttg actcaaacat tactttgctc attctcggta tcgtacttta tattttcggt
    40681 tccggtccgg tacagggttt tgcaactaca ttggtaattg gagtactcac atcactgttt
    40741 tctgctgtat tgctttcaag actcattttt gaaggtatgt tggataagaa caaaccaatt
    40801 tctttcggta atgaattctc caataacatt tacaaaaaca ttcacttcaa ctttgttgac
    40861 aacagaaaga agtattacat cctgtcttca attgtaatta ttgcaggtat tgtttctttc
    40921 gcaataaaag ggttgaatta cggtgttgac tttgaaggtg gacgcaccta caccattcag
    40981 atggataaga gtatctctac cgaagatatt cgtatggcat tggagcctca gttggttgaa
    41041 gcacccgaag taaaatcagt aggtacaaca gatcgtttca aaattactac tgcctatctt
    41101 attcacgaca cggcaagtaa tgtagaaaat gttatcgttg ataaacttta taaaggactg
    41161 acaccacttt atggttctgc tcctgatttt gagactttca aaacaaagaa tatcatttca
    41221 tcgcaaaaag taggacctac tattgcagat gatattaaac aaagcgcagt gtgggctgta
    41281 ttgatttcat gtgcgctgat gttcgtttac atctttatcc gattcaaaaa atggcagtat
    41341 ggacttggtg ccgtagttgc cttattccat gacgtattaa ttgtattgtc attctttaca
    41401 ttactgtcag gagttattcc cgtagcactt gaaatagatc agcactttat tgcagctatc
    41461 ttaacagtta tgggctatac catgacagaa tcggttgtag tgttcgaccg tattcgtgaa
    41521 tatctaggac atcatcatac tgccgatgat aaacatgtga ttaacgaagc attaaatgcc
    41581 acattaagcc gaaccatcaa cactacatta actgtattct tcgtgttgat tgtaatcttt
    41641 attttcggtg gcgaggtaat tcgtggtttc atttttgcac tcctcatagg acgtatcatc
    41701 ggaacgtact cttcgctctg tatttcaaca ccaatagtaa ttgaccttgg tggaaatgca
    41761 catgaaggtg tagcgaagaa ataatttctt aaaatatttt atttggaaaa ggctgtctca
    41821 aaaaggcagc cttttttgtg tttttaaatt tcaatgacct ttaaaaacta aattttatta
    41881 ttggaaaatt gagaattaac taagccgttc attttcttgt tgtacttgta attcctcctg
    41941 aaaaaatttg gtttattaca tctggcattt taagatatgg cgacaacttt gtacaaaaac
    42001 tgaattgtat ttcaaaagta aaatcttatg aataagcttt aataaaacat attgcacaca
    42061 tgctgtcatg ttatttatta ttttaaattg tctgcaaaaa aattgtcggt gtttacataa
    42121 ataattttta ctcttttatc tgtagcaagt tttacattgt cctgaacaaa tcctattaat
    42181 cgtttgcttt gtctgcccat gaatttactc caataaataa taacaatata gtaataatca
    42241 tttgtggttg atttttctgt atttgacagg ggttttaaaa atttcaattg tgtttttaac
    42301 gaaataatag tgtctaatgg tgcctgttgt tttggaggaa atgttttaat aattccattt
    42361 ttattccatt ttagatttgg aaatccacct gcataacagt ttatttgaaa tgattgaagt
    42421 tgtccggtct tgttgtagta aagtgcctga agaggtgggt aatggttttt gatttgttgt
    42481 ttgaaaagct tcgtgtcaaa tgactttaga aaagtaaaat atgcagtatc taattcgtaa
    42541 ttttctgttg aattaatatt gtactttttt gcataatgta gaattgtttt ttcgtcaact
    42601 tgtttgagtt tattcgttcc aattattgat gaacaacttg tcaacaagaa agcaataata
    42661 aaagcaaagg ttgacagatt tgatagttgc ttatgttttt tcattagtat gttctatcgt
    42721 tgtttaagtc atctaaaaat tgtcaattac ttccctgttt ctgcatattg cttttttact
    42781 aaatgtgctg caataaaata ggaagcaaga gcaccgatac aaaaaactac taaaaacaca
    42841 cttacaaaat cggttacctg catttgaacc ggataggctt ctacaacaaa agaatcggca
    42901 ttgcctaatt tgataagatg aaaatgttgt tgcagaaaac aaatgatgcc acctgccaac
    42961 aagccaccaa caacacctac aaaagtgatt aaaagtccct ccataaaaaa tattttttta
    43021 accattgtaa aaccggcacc caagttttta agaatgataa tgtctttctt tttctctata
    43081 acaagcatag ttagtgtgcc cagaatatta aagatgccaa ttaccatgat gaaagacaga
    43141 atgagaaaca cagcccattt ctcagaacta agaattttat aaagaaactc atgttgttgt
    43201 gcacggtttt taacagtaaa gtcatgaccg gaaatttctt ttaatttttg aatcacagta
    43261 gtttcatcag caccgggttt cagcataatt tccattgccg atgcttttcc ttcggccgca
    43321 ataaattcct gaacaaagga catgggtgcc agaatatatt tagagtcaaa atcctgctga
    43381 atggcaaata ttccaccaat tgaaatgttc atttgtcgca atgcattttc aaaatcaccg
    43441 ggagaagtaa tgtttttatc aggaagataa agcattagag gctcgttccc tgtatctgca
    43501 tttaccgaca gtgcataagc aactccacta ccaacaatgg caaagtttcg ttcaccattt
    43561 tccagaatcg gtaaaccgat aaccacagct ttgttcaacc ctgttttttt catcagttcg
    43621 ttgtcaaggc ctttcaacgt aactatatcc tgtttttcac catagcgggc aaggcagttt
    43681 tcttctaatg taaatgctgc atattttatt ccgtcaatag cacggatttt ttccagatta
    43741 agagcatcac tgttaaaagt ttttcctttt atgactgtaa ttttaatgtc agggtcgaag
    43801 gtgtcgtaaa gtgaaattac cagatttcca aagccgttaa aaacactcag cactacaatt
    43861 aatgccatag tgcctgtaaa cacaccggca atagacacaa ggctgactaa gttgacaatg
    43921 ttatgcgact ttttgccgat gagataacga gatgctattt tgataatcaa attcatgcct
    43981 gcgggctaaa gtcagcaaga ataatcactt ttacaacaac gacttaaaaa atgcccctac
    44041 ttttttacaa ccttagcttt ctgttaatag gcaataatag agctaaatag tatcttcgct
    44101 gcctcaaatt tgtaatgtga tgaaaaaact ccacatggtt gacctgatca gtcaatatca
    44161 aaaaataaaa ccggaagtag ataaagctat ttcagacact gtaacatcat cagcctatat
    44221 aaacggcccg gaggttaagg catttcaggc agagttagaa aaatatttaa atgttggtca
    44281 tgtaataaat tgtgcaaacg gtacggatgc attgcagata gccatgatgg ctcttaatct
    44341 tcaaccgggt gatgaagtta ttactgcaag ttttacttat gtagctactg cagaggttat
    44401 tgcactttta aaattaaaac ctgttttagt agaggttatt cccggaacat tttccataga
    44461 tcctgttgcc attgaaaagg ccatcacacc aaagacaaaa gcaatagttc cggtacattt
    44521 attcgggcaa tgtgccgata tggaacccat tatgaaaatt gctgctaaac ataatcttta
    44581 tgttattgaa gatgtggcac aagccattgg tgcagactac actttttctg atggcagaaa
    44641 acaaaaagca ggaactatcg gaaccattgg ttgtacttcg ttttttccga gtaagaattt
    44701 aggatgcatg ggcgatggcg gtgctatatt taccaacgat gcagagcttg ccaaaaaaat
    44761 aagaatgatt gccaatcatg gtcagtctgt tcagtatgtg catgacgaaa ttggtgttaa
    44821 ctcaagactt gacagtattc aggccgctat tttgagagta aaattaaaac atcttgacag
    44881 ttatgctgca gcgcgacaaa aagcagcggc atattatgac aatgctttta gaggtagcac
    44941 aaaaattaaa acaccggaaa gaagcaaaaa ctctactcac gtttttcatc aatacacttt
    45001 agttcttgat ggtgttaatc gtgatgagtt gcgtcactat ttagccgaga aggaaattcc
    45061 tgctatgatt tattatccta ttccgcttca catgcaaaag gcttataaag atgatcgcta
    45121 taaggaagga aattttccgg taacagaaaa actttgtgcc aatgtgctgt cattacccat
    45181 gcatacagag ttggatgagg agcagttgga atatattacc cgtgcagtac ttgaattttg
    45241 taaatagaaa ataattttat ccgtatagta ttttaatatg aacattggag ttgtaggtac
    45301 aggttatgta ggtttggtta ccggaacctg ttttgccgaa atgggcaatc atgtagtttg
    45361 tgtggacata gatcaggcaa aagttgatag catgaaacaa ggaaaaattc ctatctatga
    45421 gcctcatctt gatgtgttgt tcgagaggaa tatcagacaa aatcgtttgt cgtttaccac
    45481 gcagcttaca gatgcagtta aacattcatc aattatattt cttgcattac ccacgcctcc
    45541 cggtgaagat ggctctgcag atttatctta tgtactgggt gttgccgacc aattaggaaa
    45601 aatgctagat ggttataaaa tcatcatcaa taagagtaca gtgcctgttg gtacagcaga
    45661 agcagtgaga gatgctattg ctaaaaacta caaaggcgaa tttgatgtca tctctaatcc
    45721 tgaattttta cgcgaaggtt ttgcagtaga tgattttatg aaacccgatc gtgttgttat
    45781 tggaacacgt tcagagaaag caaaaaaaat tatgggagaa ctgtatgcac cttttcttcg
    45841 tcaaggcaat ccaatatatt ttatggacga gcgcagtgca gagttaacta agtatgcagc
    45901 aaactcattt ctggcaacaa aaattacttt catgaatgag gttgccaatc tctgcgaaaa
    45961 agtgggtgct aatgttgatg cagtgcgcat tggcataggc tctgatgaca gaatcggtaa
    46021 gcggttttta tttgccggta ttggttatgg tggaagctgt ttcccaaaag atgtgcaggc
    46081 attagaacac acatccggca agtatggata tgattttagt attctgcaat cggtgatgag
    46141 cgtaaacaac aagcaaaaaa tgttgcttgc agaaaaagca ttggaatatt atggtggtaa
    46201 tgtaaaagga aaacgatttg cattgtgggg acttgcattt aaacccgata ccgatgatat
    46261 tcgcgaagca ccatcacttt atattattga catgcttaca aaagcaggtg caaccattag
    46321 agcatttgat cctgaggcaa tgaataatgt gaaaaaactg ataggtgata aaattgagtt
    46381 tgctaacgat gagtatcatg cattagaagg tgctgatgct ttgttgatag ccacagaatg
    46441 gagtgccttc agaacacctg attttgacaa aatgcattca gctttgaaaa gcaaagtaat
    46501 ttttgacgga cgtaatttat acgaaccaca aaaaatgcag gaagcgggat attattataa
    46561 cagcattggc cgccaaacgg taaaaccatg acaaaaagaa ttttaattac cggtgcagcc
    46621 gggtttttag gatcacacct gtgtgatcgt tttattcagg aaggttatca ggttgttggt
    46681 atggacaacc taattacagg cgatttaaat aatatagcac atctctttaa gctgcgtgag
    46741 ttcgattttt atcatcacga tgtttctaag tttgtgcacg tgcccggtaa gattgattac
    46801 atattgcatt ttgcatcacc tgcatcacct atagattatc ttaaaattcc aattcaaaca
    46861 ctaaaggtag gttcattagg tacacataac ttacttgggt ttgcattagc aaagaaagca
    46921 cgcatattag tggcaagcac ttctgaagtt tatggagacc cattggttca tccgcaaaac
    46981 gaagactatt ggggtaatgt aaatccaatt ggcccgcgtg gtgtgtatga tgaagcaaag
    47041 cgttttcagg aagccattac gatggcctat cacacctatc ataaattaga aacacgtatt
    47101 gtacgaattt ttaataccta tggcccacgc atgagactta atgatggtcg tgcactgcct
    47161 gcatttgttg gtcaggcttt gcgtggcgaa gaccttaccg tttttggtga tggcagtcag
    47221 tcgcgttcat tttgttatgt ggacgatttg gtagaaggta tttttcgttt gcttcactgc
    47281 gactatgccc aacccgttaa cataggaaat ccggtagaga taactatcaa acaatttgcc
    47341 gaagaaatta ttaagcttac aggcacaaca caaaaaatta tttacaagcc actgccgcaa
    47401 gacgatccta agcaacgtca gcccgacata acacgtgcac gggaaatatt gggatgggaa
    47461 ccaaaagttt cgcgcgaaga tggattaaag attacttacg aatattttaa gtcactacca
    47521 ccggaagagt tgaaacgctc tgcacatagg tttgcttgat ttgtaatttt tcttttattc
    47581 caactagtat gagacatttt cttcttcaaa gattataatg ataaacaaat agaagaaaaa
    47641 atttatcatt tataaccaat cgaattatgt attttatttc aataactcac tttctttttg
    47701 ccttctaaca caaaacctag tgtaaaatca ttttggaatt gaagccttgt actataaaga
    47761 gggtcgtaca ggacatgtgt tgcaatacga aattgaatgt attttaaaaa cttaaagata
    47821 acagcattgg caaaatcaag attaatttga ttcttgttaa taccattagc acctcctgtg
    47881 gttactcact gtacatagct tgttgaaaaa gtcaaacata aggtggttaa tagtataaaa
    47941 aatatttttc ttagatgatg tagttgtgtt gttattgatg tagtgtttaa tcctaagtag
    48001 gcgcgagcgt catttccgct cgtgcctttt atctttgcgg cctccggccg tccgatagct
    48061 atcggacttc atagttcagc cagttttaaa tctgtaaatt gattagcact gctatgcaca
    48121 taaaaatgtg gctcttgtaa aaacatcaat ccaaccaact gtgtttaata aaacaaagta
    48181 taacccgtta ggaaatattt tgtatttttt gctcatacac cggtaaagta aggcttatag
    48241 tttgcaagat gcaaacaaaa tgaatggcac gaacggaaat gacgctcgca cctactatgg
    48301 attatttcct cttttctttt tgcctgaaat attttcgaaa caaaaaattc ttttgtaagg
    48361 cttttaaatc ttcttcaaga agaaaggaac ttttctttaa ctgatttaat gtttcattaa
    48421 aatcattttt catgatagtg tcagtaagca aagcctgtac agtaccttgt ccattgacaa
    48481 gtaggccgga aaactgattg atgttatttg ttgcctcatt taaagaatcg gaaaattgtt
    48541 gtaggttagt aagcaagatt gctaattgat tttctgaaat actgtcagca ataagtttac
    48601 ccaatacacc tttgccattt tgaacatcct tggtaatatt attaatatct tttgtaaagt
    48661 tatcaatgtt atttgtagcc gaatttattg actgaataga ttgtcttaaa ttttcaacta
    48721 cagcagaatc ggagataagt gtaataagat tgttattctt attaaaacgc tgtactaagc
    48781 ctttaagatc agatgtaatc gcagccagat tatcgttagt tttatttaga gttcttatca
    48841 tctcatccga ttcgacagga cgcagtgaca ataatacatc acctttttca attggagaag
    48901 atggagcagg cagcgagttt atattaacaa gtttattccc catcaaccca tctgttccaa
    48961 tactggcaat ggcatttttc ttaatataag gagccatctt tttttcaatt atcattttta
    49021 ctgtaatggc tgaatcactt tcaaaaatga ttttctgaac agtaccaata tcaataccac
    49081 cataacgtac attatgtccg ggcattagtc catttacgtt atgaaatact gcacttatct
    49141 caattgtttt actgaaaaca ctgcgtttgc ttccaatcat atagagggta attaccaaca
    49201 ctattagtcc cgacattacg aagaacccta atttgatatt gtttgattta gtattactca
    49261 tgatgcaaat tcaaaaaatt gattaatatt tttatcagat gattttttta attcatcata
    49321 cgttccttcc gcataattcc tgccatcaag tagaacgata attttgttag ctgtaagttt
    49381 tgctactgac atatcatgcg aaatgattac tgatgaagta tgatactttt tttggaggtc
    49441 gagcatgagt tgtacaattt ctctacttgt tatcgggtcg agacctgttg taggttcatc
    49501 ataaagaatg agttttggtt tcataatcaa agtgcgagca ataccaattc gtttacgcat
    49561 acctcctgat aattcagaag gcatcatatt gatggcatga agaagcccca cattatccaa
    49621 agattcttca actagatttt ttacttcaga agaagttaaa gaaatccagt gtcttcgcat
    49681 tgggaattca agattttctc ttactgtcat tgagtcgtac aatgcactat tctgaaaaag
    49741 aaatccaatt ttaatacgaa ttttatctag cgactcctga tctaattcaa gcatgtttgt
    49801 gtcaaaaaca tgaatattac ctttgtcagg tttcagtaat ccaacaatac attttatcag
    49861 tacagatttt cctgagcctg attttcctaa tacaacaata ttttctccct taattaattc
    49921 cagattaaaa ttattcagaa ctacattact gccaaatgct ttacaaagtc cttctatttt
    49981 cagaatgata ttagtattgt ccatcatcag ttcaagccta ataaatcagt aatctgaaca
    50041 gcaattaagt caagaataaa aataattaat gaagctacta ctacagcaga atttgcagca
    50101 tgtccaactc cttctgttcc tttttgagaa ttataacctt taaaacaacc tacaagtccg
    50161 atggttaaac caaaaaagaa tgatttgact aatgcaggaa taaaatcact gtactcaagt
    50221 gaattgaaaa ccttgttata aaataatttc caacttacta catctttaaa attaacacca
    50281 atatagcagg cgtaaatagc aattaaatca gccagtataa caagtatggg taacataaat
    50341 gcaatactca aaacgcgtgt aacaactaaa aatttaaaag gatttgtgcc agaaacttcc
    50401 atagcgtcaa tctgttcagt aaccttcatg gaacccaatt cagctccaat gcttgagcca
    50461 attttacctg cgcagattaa tgcagttatt accggcccta tttcccgtac aattgaaata
    50521 gcaatcattg ccggcaacca tgatgttgca ccaaattctg ctaatgtggg tcgcgattgt
    50581 acggtaagta caagacccat aatgaatgct gtaataccta ctagtggcaa cgacttatag
    50641 ccgtttataa atgcttgttt aaaaagttcc tcccattcat agcgtggttt aaatccttct
    50701 ttgaaaaaac gaacaacaaa ggacgacaat tgccccgttt catgtaataa atcagatata
    50761 cctttaggca gcttcataat tcaaattcca agtaagaaaa acactttcat aaaggagcta
    50821 agataaaaaa ttaatcggag caatagttta ataaagtgaa taaacctgta ggttaaccac
    50881 aatttactac atttctaaaa acagagtttt cttagcatct ttgcacaatg gcagataaag
    50941 aaattaccca acccatacag accgattccg gcattgaagt aaaacaggtt tatacatcat
    51001 gcaaaccaat ggacgaacta cctgcaaagt ttccatttac acgtggagtg caagccgata
    51061 tgtatagaag caagttgtgg acaatgcggc agtatgcagg attttctact gcagaagaat
    51121 ctaataaacg ctatcactat ttgctgaaac aaggcacaac aggcttatca gttgcttttg
    51181 atttgcctac acaaattggc tacgactccg atcacgagct ttcagaaggt gaagtaggaa
    51241 agtccggtgt ggcaatagat tcattgcgcg atatggaagt attgtttgac ggtattcagc
    51301 ttcagaacat cacaacatct atgaccatca attcaacggc tgctgttttg ttgagcatgt
    51361 acattgcttt ggcgaagaag caaggagcag acctctctaa aatatcagga acaatacaga
    51421 acgatattct gaaagaatat gcagcacgcg gcacatacat ttatccgcct aaaccaagca
    51481 tgcgcatcat tacagatatt tttgatttct gcagtcgtga agttcctaag tggaatacca
    51541 tatctgtttc cggttatcat attcgtgaag caggaagcac tgctgtgcaa gagttggcat
    51601 ttaccttagc tgatggcaag gcttatctta aagcagcgat agatgcagga ttggatataa
    51661 atgtttttgc caaacgtatt tcttttttct tcaatgcaca caacaatttt tttgaagaga
    51721 tagccaagtt tcgtgcagcc cgtagaatgt gggcatctat cacaaaagag cttggtgcta
    51781 cagaccctaa agcaatgatg ctgcgttttc atacacaaac cggaggcagt acattaacag
    51841 cacaacaacc acaaaacaac attatgcgtg taaccatgca ggcattgagt gctgtacttg
    51901 gtggaacaca atcgttgcac accaatggtt ttgatgaggc attatcatta ccgactgaaa
    51961 atgctgcacg tattgcattg cgaacacagc aggttattgc ttatgaaagt ggtgtaactc
    52021 aaacggtaga tccgcttggt ggttcttttt atatagaatc attaactgat gaagtagaag
    52081 ctaaggcttg ggattacatc agaaaaattg atgatatggg aggcgctgtt gcagcaattg
    52141 aagcaggcta tattcagaat gaaattgcag agtcgtcata taattatcaa aatgcaattc
    52201 aaacgggaga aaaagtaatt gtgggagtaa ataaatttca ggttgaagaa ccggtttttg
    52261 atgatatctt ttctgttgat gacaccatca gaaaagtaca agtacaaaaa cttgcacagt
    52321 tgcgcacaga gagaaataat gaaaaagtta atcaggcatt aagcaactta gaatctgctg
    52381 caaaggataa tgccaatctt atgccttaca ttattcaatg tgttgaacac tatacaacac
    52441 ttggagaaat atccgataaa ctcagaaaag tttttggtga gtatcgttga taaaatttaa
    52501 tgacattgta aaattcaaat catatataat ttttaaaaat gaaaaaaaga attttaatta
    52561 ctcttgctgt ttcaacttta tttttcactg cttgtaagaa agataaagac agcaagcctg
    52621 ctgaaagcga gttatcgttt catctgcata ccttagtagg aaacactgct gccaactaca
    52681 ccgatatctt tactgataac accggaagaa aatttaatat tgctgattgc agatattaca
    52741 tcagcaacat tgttttgatt aaagatgatg gaacggaatt tccgttgacc gataaagcgc
    52801 tattggcaag tccatcacaa aaagaataca gtttgggaaa tgttccggtt ggaaaatata
    52861 aaggttttcg gttcttaatg gggttagact cagtgactaa tcatgacgac cctgcaggtt
    52921 atgctgcatc aaatcctttg agttatcaga atccaacaat acattggggc tggaacaatg
    52981 gctacatctt tatggagttt gtaggcaagt tcgattcaac agtcgctgca acaggaaatc
    53041 cggtggcaga ttatttttat cacattggtt ttgatgattt aaagcgtact attgatttta
    53101 gcaattctcc ttttgaaatt actactgatg ccggtttaga gattggtatt ttattcgacc
    53161 ttaaaaaagt actgaacaat gtagatttac gtacagagtt tggtacacat acaatggata
    53221 atatgcccct tgctgttaaa attgccaaca actggcaggg tgcttacagc attgaataat
    53281 ttactatctt cgatgtatta atattttcta tcatgaaaag tatcaagtct gttttgtgtt
    53341 tagctgttgt tggttttatt ttctctttta gctcttgcaa gaaagatgat tcagatgcta
    53401 ataatatacc caacaccgac accattggtg agttgtcttt taacttcaga cagtttgttg
    53461 gcaacgacag aatagtttac agtcaggttt ataatgacac atcagggcga ccctttaaaa
    53521 ttaccaacct gcgttattat atttcaaata ttgttttaat aaaggcaaat ggtctggaac
    53581 ttccgcttag tggaaaagtg ctgttggttg acaatgaaaa agagcaatat actcttggtg
    53641 aaattcctgt tggtgattat tccggtttgc ggtttatggt tggtattgat tctgcaacca
    53701 atcacctcga ccctgcattg tacgatgcat caaatccgtt aagttatcaa cccgtgagta
    53761 tgcattgggg ttggaacttt ggatatattt ttatgaaagt agaaggcaat gtagatacag
    53821 atagtgttcc tacaggaaat ctaaacggaa taatttcata cgatattggt agcgatccat
    53881 tggtgcgtgt gataaatttc aaccatattt tttctgtgca tggtaatatc aacacagtca
    53941 actttaaatt taatatcctg aaaatgttta gtggtattga tttagcaaca gaaagcatca
    54001 cacatacaaa tccgggtaga gatttagcag acagaattgc cagcaattgg cttcttgctt
    54061 tcaccattga accatgatta aaatacaagt atcaaaaatt tttattttat cagttattgt
    54121 tttttggata gtaagcattg tatcatgccg aaaagatgat gccattggca caggaaacga
    54181 agtactggaa ttggaagtac ctttaggatt tccctatcct tcaataccgg aagataatca
    54241 acctacaaaa aacagaattg ccttaggcaa aattctgttt tttgacccaa tactctctcg
    54301 cgacagtacc atttcttgtt ccagttgcca tcatgctgat aaaaaattta ctgacggact
    54361 taaagtcgct gaaggaattg aaggacgaaa gacattacgc aattcaatga caattctcaa
    54421 cacggcttat cagccaaata tgttttggga tggtggctca ccgacattgg aaacacaggt
    54481 ttctgcacca attgaagcgc acgaggaaat ggattttaat gtcatgaaag ttgttgaacg
    54541 tttaaataac catcctttat atccatcatt gttcaggaaa gcatataaca gcggaccatc
    54601 agcatttaca cttacaagag ccattgcaaa ttttgaacgt acactcttta caggcaaatc
    54661 aaagtatgat gcgtttcagt atgagaaaaa aagcgatgca ttgtctgctt cagaaaaaag
    54721 aggtatggat atattttttg gtgagagagc agaatgtttt cattgtcatg gtggatttaa
    54781 ttttacggat tttagcttta agaataacgg actttatgaa gtttatgcag attccggcag
    54841 agcacgtata acaactttgc atgatgatgt gggagagttt aaagtgccat cacttcgcaa
    54901 tgttgcactc acagcaccct atatgcacga tggcagtgtg gctactcttg aagatgttgt
    54961 tgaacattat aactcaggag gtaagccaaa ttttaataaa agttttctca tcaggccatt
    55021 gaatcttaca caacaggaaa aagaagactt ggtgaatttt atgaaagcat tatccgatca
    55081 ataacaatct catttaattt agcatcatga aaaatattgc attatacatt ttaacttttg
    55141 gaatgacagt attccttgca tcatgccata aagacgacag ttgcaatgcc ggtaccggtg
    55201 gaaataattc tataaaggca actttgttgc atcacactaa ggttataagc ccaaaagtgg
    55261 gatatcctga tacacttttt ataaaatacg acaccaaaga ttttccgggt gaaaatgctt
    55321 ctgattttga tgcttacttt gttaacagcg atactgctat aagcactatt gaaattacag
    55381 gtttaaaatg tggtgattat tatttgtttg gaaccggttg ggatgctgat atttctcaac
    55441 gtgttaaagg tggcattccg gtaactattg cagaagatgc aggcgtgctg acaaagaatg
    55501 ttccggttac ggaagattag tactaagaaa gatattttcc cacaataggc attcgtctgc
    55561 ctaagccaaa cgctttagat gaaatgcgca atacaggcga caactgtttg cgtttatact
    55621 cattgcggtt aaccatactc aaaatacggt ttactaaagt ttcatcatat ccgcctttta
    55681 taatttcttt cgggccttta cgcagttcta tatattcaaa caaaatttta tcgagaatat
    55741 cataaggcgg taaagaatca ctgtcctttt gccccggacg taattctgca cttggagctt
    55801 tacttaaaat attttcagga atgatttctt tttttctgtt gatgtatctg cacaattcat
    55861 aaacttctgt tttgtacaaa tcgccaatca cactcagccc gccacacatg tcgccatata
    55921 aagttccata gccaacggca agttcacttt tattgcttgt gtttaaaagt atagggccaa
    55981 atttattact cagtgccatc agcaatgttc cgcgtatgcg tgcctgaatg ttttcttcag
    56041 taacatcaaa aggcttttca ccaaacaatg gtttaagtgt tttcaaaaac gtttcataca
    56101 atggatttat ttccatttta tcaaccttga tgttcaggtt ttgacaaagt tgttcggagt
    56161 ctgatattga atgtccgctt gaaaaagctg aaggcaacat aactggcaaa atattttctg
    56221 cacccaaggc atcggctgcc aacactgtaa caatggcact gtccacacca cctgacaagc
    56281 caagtatcgc tttcttaaaa cccatcttag aaaaataatc tcttatcccc atcagtaatg
    56341 cctgataaat caattcatat ttttctgttt gcttttcaac aacaagagtg gtgttttttg
    56401 tattcagata aattatttct tctttaaaat aaggcccctc atgcaccaat ttacccgaag
    56461 catcataagc taatgagcca ccatcaaaga taatttcagt ttgtgcgcca acatggttca
    56521 catacaataa tggaatttta tactgattta catttcgctg caaaattgct tttcgctgat
    56581 gtgcatgttt gtaatcaaat ggcgaagctg caatattgat gattaaagaa ggtttttctt
    56641 ttatcaactc ttccatagga gaatgaatgt agagtggatc gtcttccaca ttccataaat
    56701 cttcgcaaat agttaaagca attttttctc cgttaaactc aatacattca aattttctgc
    56761 ccggctcgaa atagcgatac tcatcaaaaa tatcatatgt aggcaatagt gctttgttaa
    56821 ctaaagcctg aactttgcct ttatgcaaaa accatgcagt gttaaagaga tttttccctt
    56881 cgggaacggc attgcgcgat gggcaaccaa caatgactgc aatattctga ctgtgtgctg
    56941 caattttttc tatgctgtta taacaacggt tgatgaaatc atcaaactcc agaaagtcga
    57001 gtggaggata tgctgttata gccagttcgg gaaaaacaat cagttctgca ccggattttt
    57061 cggcttgttt gattgcctca ataatttttg aagtgttgtt ctcaaagttg cccacatgaa
    57121 agttgagttg ggcaagtgca atgtgcatat ttcgaaatta tcaggcaaag gtatatttat
    57181 ataaataatg ttacagcgtc tttctgatat atctttttaa tgtaatttaa tcttccgcac
    57241 ggtcatttca agaaatgaca gtgccacaaa taaaaaaagc tgcctacaaa gggcagcttt
    57301 ttataaaatc ttattgtaat atttaaaaca tcacacctaa atcaacagct aaaaagtttg
    57361 acgttgcttt tgaatgtttt tctttaacta catcaagaaa accattatta aaagttatac
    57421 caaaaagtaa ggctgttttt ccacctaatg aatattgtgt tccaataccg gcaactaatg
    57481 ccatgttcaa tgggttgata tccgattttt taaaatcacc atctgttctg atattgatac
    57541 ccgttgtaaa tcccacctta ccaaagtaag tcatataacc aatttcattg gttttcagct
    57601 ttaaggttaa aggaagttca atgtattgca atacggtttt cattttatag atgtccatat
    57661 atttcaattt gccaccacgg taggtaacat caatgccggt ttcaaatgca tagttgttgg
    57721 taaaattaat atcggcaatc agtccataag caaaccctat tttgctacca tcattatcta
    57781 aattatcgct ggctttaaac caggctaccg ttggtgaaat tttaagtccg aaattaaatt
    57841 tgttgtcctg cgcttttgca aattgaaaag cagctacaaa ggctattgca attagtgata
    57901 cttttgctct cattatttta tgttttttta attcaaggca aaattaatga aattttccac
    57961 agtcagacaa acagtttatc ttttactatc aacagtgctt atgctgggtt tttcttgtca
    58021 gaacgacccg catggagata aacatactaa agacacaatg accacacatt taaaatttga
    58081 tgaagtgttg ttttctgctg acagtattaa cccacagcat gtggtgcaat ggaaacaaca
    58141 atatccttat tttttcaatc tgtttgtaaa cagaataata catctgccgc caacagacag
    58201 tgctgcactt gcattaaatc ttcaaaaatt tgttggtgat aaagatgtaa aggacattca
    58261 tgctcgggtt gagaaagttt tttctgctgc cgataaagat aaattgttct ctgaaataga
    58321 aggattttta aacaattaca aaaaagagtt cggcatacaa ccggtaaaac atctggtaac
    58381 ttttctttct gcatttaact atgctgttat aactgcagac agtaccattg gtattgggct
    58441 cgacatgtac ttaggcagcg attgtgagtt ttatccatca ataggaatac ctcaatatgc
    58501 ttatcaaagg ttttcaaaag attatatttt gagtgatgtg ataaaaggct ggtatcagag
    58561 tgaacatgat gtggatgcag aaaaaaatga tttcctcagc cgcatgattt attatggcaa
    58621 gcaattgtat tttacttcag ttatggcacc acaagtgcaa gacactattc tgactggata
    58681 cagcaaaaag caattggagt ggtgcagcaa caacagcaaa cagatgtggg cattttttat
    58741 agagaacaaa cttttttatt ctacccgcga aatggaatac atgaaataca ttgcagacgg
    58801 acatgccaca caaggttttc ctgaaggggc acctgcacgc acagcacagt ggctgggatg
    58861 gaagattgta agcacctata tgaagaacaa caacgttaca ttaaaagagt tgcttgcaga
    58921 aaaagatgca cagcgaatac tcgaaaaatc aggatataaa ccttagttca aatttaaaat
    58981 aacaacaatc agaaagatga aaaaatcaga gataaaattt actgtaagct tagacgacaa
    59041 caacaaacct gtggcattgc attggaatgc cgaagacagc ggcatgcaag gcgacaaaga
    59101 atgtaaagcc atgatgcttg ctttgtttga taagaaagat caaactacca tgcgcattga
    59161 cttatggact caggaaatga tggtagaaga aatgcaacag ttcttttttg aaatgtatat
    59221 gagcatggcc gatactttcc agaatgcaac taacaataaa gatatggccg ataaaatgcg
    59281 aaactttgca agacagtttg gaaaagaagt gagtttgatt aagtaactat ttcttaattt
    59341 cttattcttt tttatttggc agaattctgt ttcactgcaa aggcaataat gtgtccatca
    59401 ttatctgcaa aatagcaaac tttatcgccc caatctctgt ctgcaatagg gctaataagt
    59461 tttgctccac attttagggc atttgcatat tcagcctcaa tatcgtcaac ataaaagtat
    59521 aattcacatc ttggaattcc ggtacctttg ttgggatgtg gcattttttc agaaatgatt
    59581 tttgcaatac cattatcagg catcaaccct aatttgcaat taggagaaag tataaactct
    59641 gtcattcctg atgcattcaa atccggaatt tttctaaaga gttttgaata gaatacagtg
    59701 cttgcctgca gattgtttac ataaagaata aattctgtgt atagaatatg tgttgtcatt
    59761 ataggatatt ctgaatccgt ttcatagtca ctgcgaaggg gcgggagatt atgaaactac
    59821 aaaggtactt agaaacttat gatattgaca acatcaaaga tgcgaaacaa tttttaaaga
    59881 ctgtagtaga tctatacaac agcgaaagac ctcacatgag tatcagtaac tttacaccaa
    59941 acagtattca tcattcaaaa acaacaatca aaacagaaag attatgaaaa aattattatc
    60001 gaaaaattcc tactattgta aatccaattc aggactaaaa atgaattgta aacttatatc
    60061 aggattaatt aattaaactg taaacttttt ttaggacgag tcatttgctt ttgctgcaac
    60121 ccacgaaccc aacgcaaagg caaaatcaaa agagccgctt tttgccaagg cttttaaaaa
    60181 agcagcagtc agaattatat ttttgctgat atttaagaac caaaaattgc aaagtaatta
    60241 ttatggactt tgatgcacta ttaaaacttc ttcaagattg gacaaaccga gttccgctaa
    60301 tcattctagg cagtggtgca tctgttccgt ttcatcttcc ttccatgtgg acacttggcg
    60361 accacttgaa gaagtcaatt tcatttgccg atgccgatga caagaaacag tttgaagaat
    60421 ttaaaacaac ctttgacatg gttggcgatt tagaaacgac tttgctgcaa ttacaactca
    60481 gaccaaatgt tctaaaagaa atcatttttg aaacttggcg attagtaaac aaacatgacc
    60541 ttgaagcata cgagaaaatt ctaaacaaga aagttgagtt tccgcttgct gacataacaa
    60601 ggcatctcat ttcagctgca cagagaaaac tttcaatcgt cacaacaaac tacgaccgac
    60661 ttgctgaata cgcagcaagt ttatcacatg cgttcatttg cacaggttat acgcaaaact
    60721 atttcggaca tttttccaag ttgatacatc aacaggattt gaccaagttg aacggcttta
    60781 gcggacaagt taacatctgg aaagttcacg gctcattgga ctggttcaaa tcaaaagagg
    60841 aagttgattt tcatttgcca ttgcgacaaa ctattccaac tgacttcaaa ccatctatcg
    60901 taacacctgg gctttccaaa tatttggaaa cgcaattaga accgttcaga acaatattta
    60961 cagaagcgga cagtgaaatt gaaaaggcag tttcttattt gtgtattggt tatggtttca
    61021 atgacattca tgttcagcca aaacttctca cacaaatcaa aagcggcaaa ccaattattg
    61081 taatcacaaa ggaacttaca ccaaaaacga aagaatcaat cattgacagt aaatgcaaac
    61141 agtatatttt gattgaagaa gcaaacaaga aggatacaag aatttattca tccagcttcg
    61201 ctggtgaaca aattgttcct gatgtcagct actggacatt gggagagtat ttaaaactta
    61261 tcaaatcgta agacatggca atttttaatt ttcaaaacga tgcggcaatt ggaacagtgt
    61321 tcagtgtaga cactgcaact gtaattatca aggttgatga aattgataaa ctgcgaaagt
    61381 tgcatgtcaa tcatttggtt gctatcaaga gttcaaaagc gggacaacat ctgataggta
    61441 ttatcaatcg tatcacacga aaagcatctg acgaagaaaa accaaccgaa gaagatttgg
    61501 gtttaggtgg gtttcttttc actgaaaata ttttgaaagt aaatcttatc ggaacacttc
    61561 atgataagca cggacagaaa gaaaatgttt tcaagagaaa ccttgacaca gttcctgaaa
    61621 ttgaagcaca atgttttcct attgacggag aaaacataac caagttcatg acaacaattt
    61681 caagtgttga caattataaa gtccctctga caattggaaa atattctatt gacgaacatg
    61741 cacaggcatt tttagatgga gacaaacttt tccaaagaca tgcggtaatt gttggtagca
    61801 ctggttcagg taaatcatgg tgtgttgcaa agttccttga acagattgca caactaccaa
    61861 tggctgattg cattttgttt gacattcacg gagaatacag cggaaaagat tttaaaatgg
    61921 atggtgtgca acacttgcgt gttgccaatc catctgactt aggtaagaaa gggaatcttg
    61981 caaataatgt tttgatgttg ccctactgga tgctcactta tgaggaaatg cttgcaatgc
    62041 ttcttgacag aagcgatagc aacgcaccga atcaagcaat gcttttttca aaaaccgttt
    62101 ttacggagaa gcaaaagttt ttggatgcta taaaagataa aatatttaaa gaccacatta
    62161 cgattgatag ccccattcct tacaaaatgg aaaatgtttt ggatgagtta cagaggatgg
    62221 atgaagaaat ggttgctggt gctaaagtgg gaactgaaaa gcaaggaccg tttcatggaa
    62281 aacttacaag atttattcaa cggcttgaag caaaaagtca agacaagcgt ttaggatttc
    62341 tgtttcaact ttctgaagat gaattgcaga ttgattggat gaacacactt tgcgaacgct
    62401 tgatgttcgg ttctgcttta agcaaagaaa aatcaggagt aaaaattatt gacttctcag
    62461 aagttccttc tgatgtattg cctcttgtaa tcggattagt tgcaagagtt gtatttacag
    62521 ttcagcagtg gacagcaaaa gaaaatcagc accccatttc attactgtgt gatgaagcac
    62581 acttgtatat tcccgaaaga acaaatcaag atgcagcagc agaattaggg ctaaagagtt
    62641 ttgaacgaat tgcaaaagaa ggaaggaaat acggagtgag tttgactgta ataagtcaaa
    62701 gacctgcgga agtaaaccga actgttttaa gtcagtgtaa caacttcatt gcattgcgac
    62761 tttcaaatgc agaagaccaa gcggtaatca aaaaactttt gcccgataat ctttcgggac
    62821 ttactgatgt tttgccgata cttgacattg gagaagcact tgtagtaggt gatgcttcat
    62881 tgcttccaac aagagtaatt attgacgaac caaaaattaa acctgaaagt gcgacaatta
    62941 aattttggca agagtggagc aatgacaaag caaaacaaga tattgcaacg gcagtaacag
    63001 gattaaggaa acaatcaaaa gcataattaa aaaatataaa catggcagaa gaatatttag
    63061 acatctacaa ctcaaaagga gttcacttag gaaaggaact caggaaagta gttcaccaaa
    63121 aaggaatgtg gcacaaaaca tttcactgtt gggtgatttt ccgaaatgaa gcaggtgagg
    63181 attgtatcct tatgcaaaag cgagctgcca aaaaggaaag tgctccaaac aaacttgatt
    63241 cagcagtagc ggggcattac acagcaggag aagatatttc gggaggcatt cgtgagttta
    63301 aagaagaaac aggaattgac attgattcta aagatttaat tcctcttgga atcagagtta
    63361 ctgtttctga ttacaaacca aacagcatca acaaagaatt tcaagaagtt tacttcatta
    63421 agaagtcaat ccctttagaa tcattcaaac tcccagttga tgaagttgca gggatggttg
    63481 aattgccagt caaaaagtgc atcgcacttt tagacaaaca gattgaccac ttttttgcaa
    63541 ggggaatttt taatgatgat gcttcatcac cgaacatagc aataatcata gaaagaaaaa
    63601 tcacgattga cgattttatt tggttcattg acaattttca tttcaaaata atgctgctaa
    63661 caagcaaagc attagcgaac gaaaaatatc tctttatcta atgttacttg aaaacctgag
    63721 catagaaaaa cttccctatt tggagcggta tgtaaacagt ggttcgccaa gtggttttac
    63781 aaatcgttac actacttccg aagaaacaaa accctttggg ttatcgcctt tttttcatcc
    63841 tttgatttgt gtcgcacctg aagaatgttt taaaaactat ggcaagatac cagcgttcaa
    63901 agagtttgaa gaagttacag gaaaaaattg gttgcttgtt catcccgaca tgaaaaaaga
    63961 gaaggtattt aaagcaaaaa atgtttcact tacaaaatca aagaagataa aattagttcc
    64021 caccgcaagc ggaagaacag ttgagctttt taattcatca accaagggtt atttcaaact
    64081 tcattacaca catgttatcg gacgaataac aagagaattg gataataaaa ttggagttgc
    64141 gggacctgaa atttcaaagc atcttgtttc cttgatagac caaaacaaac ttgacagcag
    64201 attaaccttg atgcgtgaaa ctggtgcaag aatttttcag gggcatgtaa acgagaaacc
    64261 tgttgagtgg ggaatggttt ggcgagagaa agatgcttac aagattgact acaacgaagt
    64321 gaaatatgta attcctgttt tttcactttt tggtaaagac atactggcga aaagagataa
    64381 gccgctattg cagcaaatca ttaatctcat cggagcaaac ccaactgact atgttttaaa
    64441 gcaactgatt ttcccaatga ttgagcttta cttcaacttg attaaaaact caggcattca
    64501 acctgagtgg catgctcaaa atttgttgat aggttttaca aaagattttg ctcctgtaaa
    64561 atttatcatg agagatttag aatcaatgga caaggactta acattcatgg aggaacataa
    64621 catcaaggca aaatttgaaa gttatccctt caaatgtatt ttcagagagc agtggaatta
    64681 tcaaaggaag cacagtttta tgtatgacta taagtttggg gaatacatca ttagaccgct
    64741 tctggacttt ctaaagaaaa aatacaaggt tgacacattg cccttacatc aaaagataaa
    64801 acttcattca aagaaattca ttaatgaatt ttcaaaagat ttttttccag agtattgggt
    64861 tgtttttaga aagattttga ttaaccaaaa gacagataag cgtccgtatg ttcataggaa
    64921 gaatcctaaa ttcagatatt aaatgcacat agtttttatc agacataccg aatcagaaaa
    64981 gaatgtaaac aaccagttct catcttacaa ggataaagaa ccgttgactg aaataggcat
    65041 gagtcaaagc gaaaatcttg gcaacgattt attggagttt aaagaaagaa aaaacttgac
    65101 ttgcaagaat gtttatgcag caagttctac aagagcaatt gaaacagcaa aaattattgc
    65161 ttctaaatta aatgctactg taagtgttga ggatgctctc cgttctacaa agccaggagc
    65221 attatcgggc aagtcagaag aggaaggatt aaaaacaaat cccgatttct tagaacaact
    65281 ttatctattc agaaacgggt tgttcaatgc ttacgacttc actgtagcgg aaggaaaaga
    65341 acccaaaaaa gattttgaga aaagagtttg gggaagattg agtgaaataa tatctgacaa
    65401 gtcagagagt ttgaaaatta tctctgcaca tcgttcatcc attacataca tgctgttgga
    65461 gtttgcaaaa agatattaca aataccctca aaatatgtca gggcatgtat tacttgattt
    65521 gggtaaaata tcacttgtgg aaactgtagc agaaaataaa tggaaaatta caagagtgaa
    65581 ctttgatagt tccgaactaa aaacactttg atatatgagc gaaaacaaaa caaacaaaca
    65641 ttcctattta tcagcactaa tccaacttgg aaaatttgcg tggggaatat tcattggcat
    65701 ttcaatattg gttacagtgg cgacaatggt aacggacttt aaaatgacaa aggaacatcc
    65761 tgttctcact ggcttggctg ttcttgctgt aacgattttt atttcactca ttttattggt
    65821 gataaaaatg attaatgaag gcgttgaaca aaaatcccct ttacctcttt ctaccgaatt
    65881 ggtgaatata ataaacacac tttacgaaaa ggaaaagtat ttagatgtag tgcggcttgg
    65941 ttctgcttta agcagatatt tatggctcaa aggaaaatat catgaacgaa ttgaaattgg
    66001 tgaacgagtt gaagatgcag caggaaagga aagtcgcatt gaagaacaag tatcatctct
    66061 gatagatgat attggctgga cttacaacat tatcggacaa cccgataaag cggttgagaa
    66121 tatcacgagt ggaattgata aagctcttac aaacgagtta ttttattatg ctgctaaagg
    66181 tgaacgacat ttatctggaa ttgaaaaaca caaaggacat caagccgaat gcttgagcca
    66241 tttacaaaag gcaaaggaac attcagaaaa gattttggat ttgaccagta aaaatgaaat
    66301 ggaagccagt ttgtttttag cagaagcaaa atatctgttt gaattaggaa aattaaatga
    66361 ggcagaagca cttgcagaaa aagcaatagc agtatttaaa aatgaccccg ataggattgt
    66421 aaagattcat tcgctgttag gaaatattta tttgaaacag aacaaacttc aaaaagcgaa
    66481 agatgaattc aataaaggat acaatgcaac aaaaagcaca agagaagatg aggttgcaaa
    66541 aaacttaaaa ggtttagcag acatcgcatt gcttgaaaga gacataaaaa gtggattaaa
    66601 atatttgaac gaagcaaaag aaatctatct ttctattcac aagaacaaag aggcaactga
    66661 cttggataac atcattcgca gtatcaggat ttaagcaatt aagatttagg acaaatgcca
    66721 agtttgattt taacctcaga tgattttggt ttaagcaaaa tatttaatca gcgaatgatt
    66781 gaattgctgc aatcaaattt tctttcgtca atttcaatca tgataaatca agtaacttcc
    66841 gaccaacaaa atcaaattgc cgagataaaa aaaatgtatg ctaaaaataa tttcagttta
    66901 ggacttcatt tagaggtttc agaagcagac gatgaagcga catatcaaag ccaatggaat
    66961 atgtttgaac aattactttc aataccacct gactacattg atgttcacaa agggcatttt
    67021 caaaatgcaa ccttcaactc tattgcagag ttttgtttgc aaaaaagagt tcggttcaga
    67081 aaatacaaag agacgacagt aaatgttagc agccctgaaa gttctctcac tgctacattc
    67141 aaagagttgg gtgcaatcaa agaatggatt gcaaacttca aaacaaactc aacttatgaa
    67201 ttggtttttc acatcggtag ttttgacccc gacagtaaat ccagtttgaa taaagacaga
    67261 gaacttgata ttgaaaagtt aaaacatgtt cacgaattta tttcagacaa aaatatttca
    67321 ataacaaact acaaatcgct ttaaagaaaa tgccaacgca gcattcacac acatttgcaa
    67381 gccgctacaa gccaacgctg aaaccaaaac ttgcaaaaga gtgtgtctgc cctacccaag
    67441 aagaatttga aaattttttc tccaccccct caaataaaat taaatcaccc aaccgcacag
    67501 tgctcactcg gacagacatc gccttgaatg taaatcgcct tgtgggacag gtttgtgcag
    67561 acaatggaca gacactattt cattgcgaga agtcgggtga tacgcataac aagggcttaa
    67621 acgaaagcgg gggttccgtg cttcgcagac acatttgtgc aaggtggaag ttcagttctc
    67681 cgaatgaagt ttagtgctac aactcaccct ctggcgcaag tcttcggact tgtgcccatc
    67741 tatcattcaa gcagcagtaa gtccaataaa cctttggtgc cgcaataatc acatgcactg
    67801 ctgtaaaaat aatcttcctt gtaggcgcga gcgtcatttt ccttactttc aaagcctctc
    67861 gcatttcagg ggactttgaa ataataaaaa tagcattgaa gggttaatac attttttatg
    67921 aatatagaat acatgtagtt taaaattttg catgacgact acacgtagaa aatatgatga
    67981 acgtgaagta cggcattatc gctctcccaa ctggaacaaa ctaactttgt tttaatatga
    68041 tatagtgtgt acctgatagg ttcaatccac atttttctgt cgcagttttt ttatttctgc
    68101 tgttctgcgt ttttgagtca gtcgttcttc ttttacctgt gccggtattt ttgtacttat
    68161 tcgttttgca ggtctatgca tagctttttc aatcagcatt tgaaattttt tcaataccag
    68221 ctctttatta cctaactggc tacgtgcctc actgctttga agttttaaga aaccatcttc
    68281 gttgattctt cggcaaagtt tttccgtaat aacttttttt tgttcctcat ctaaacatac
    68341 agagttttca atatcaaagg caagttcagc cttggtagaa accttgttta cattttgtcc
    68401 accttttccc gagctgcgcg agtaggtcaa tatcacttct gaaaaaagac atttaaaatc
    68461 tatttttccc attacagttt aatggtaaaa ctatcggcat cattgcccac aggaaaaatg
    68521 tgccttgcat tttccaaaac atcaaagtcg agttttacag tttctatatt ttctgttgca
    68581 gattttgttt tgctgatttt tttaccaaac ggatcgagaa ctactgtatc tccggaataa
    68641 gtaatgtcat ttccatcttt tccaatgcgg ttaacaccaa caacaaacga ttgattttct
    68701 atggcacgtg ccgtcaacaa ttgtttccag gcaaagctgc gtttctcagg ccagttagct
    68761 acaacaatag cacaatcata ttcgttctga ttacgcatcc acacaggaaa acgcaggtcg
    68821 taacaaataa aaactgagag tcgccatcct ttataaagca gaattaattt cttgtttcca
    68881 ggatgataaa atttttgttc accagccata gtaaacaaat gccttttatc gtaatgtgca
    68941 tgagcgccac cgggagtcat ccatactaag cggttataat atctgccacc ttcttcaaca
    69001 ataagacttc cgcaaactac agcatttttt gtttctgcca ttttttgcat ccactgcatg
    69061 gtagggccat catttttttc tgccagtacg cttgcattca tgctaaagcc cgtactaaac
    69121 atttcgggca gcacaattag gtctgtgcta cctgctttaa tacttttaag cagtgtagta
    69181 aaatgtttaa tgttttttgc ctgactttcc cagtgaagct ttgtctgaac agttgttact
    69241 cttagagtgc tcatggtatg atgttgttgg ttcaggcaaa gttatatttt ataattgcag
    69301 aaaaggtatt atttttcaac cttatgcagc ctttcagcag ctttttcaag tgtttcttca
    69361 gattttgcaa aacaaaaccg caaaagtttg tggtctgtgc cgtttctgta aaaagccgaa
    69421 accggaattg atgccactcc atagtctttt gtcagtttta cggcatagtc catatctttt
    69481 tcattactca gtttactgta atcgagcaat tgaaagtacg aacctgagca ggccaatggc
    69541 ttaaatgcag tgccttgaat cagctttaag aaattgtctc ttttttcctg ataaaatttt
    69601 tcaatacctg aatagttttt agcatccttc ataaattctg cataggcata ttgaatggga
    69661 gtgttggcac taaaaaccat aaactgatgt gcttttctga attcggcaga aagttttgcc
    69721 ggggcaatgc agtatcccat tttccaccct gtggtatgaa aagtttttcc gaaagaataa
    69781 acgataaaac tgcgctcggc aagttttgga aaacgcgcta cactctgatg ttcgtaacca
    69841 tcaaaaataa catgctcata cacttcatca ctcaagacaa aaatgtctgt ctttgcagta
    69901 attttttcaa gcatctgaaa atctttaaca cttaaaatag cacctgtagg gttatgtggt
    69961 gtgttaatca taatcattct ggtgcgttgg ttcatgagtt ttttcacttc attccagtca
    70021 atatggaagt cgggcatttt taactctgca tgaaccggtt cgccaccggc aagaacaaca
    70081 gcaggtgcat aggaatcata agcaggatca aaaataataa cctcatcgcc ttcgcgtact
    70141 acagatgcta tggctgtata aatagcttgt gttcctcctg cagtgatagt aatttctgtt
    70201 tcaggattat attttgcact aaagcgggct tctattttag cagcaatttc ttcgcgcagc
    70261 ggcatgatgc ccggcatggg tgcatattga ttcatgcctt ttttcatata cttatttacc
    70321 agactaatca gttcgggcga aatggcaaag tcgggaaagc cctgagaaag attaatggca
    70381 ttacactcgt tggcaagtgc agacatgaca ctaaaaatgc tgtttccggt tttaggaagt
    70441 tttgatttta ttaatgcttg atttggtgac ataaaaagcg gctatgcttc aaatgtaaat
    70501 caaatgccgt taacaaaaaa gaaatctgct ttataagctc ctgctttaat gctattgtat
    70561 tgagtataac agcaaatcgt tttaaacaac attccctttc acagattttt atttaagatt
    70621 ttgatggttg tagaattgaa gtcttttgtt tatagttagc agtttttaaa atcttcattg
    70681 atttgaattc aattatattt gtttgctcag ttaatgtttg tattttgatt gaatgaaatt
    70741 tccatcaatt aatttttctg tttatggtaa aagaacatat ttggttcttg taagtgtatt
    70801 gatcggtgct ttaactactt tatgtgcagc cttattaaaa aaaggtgtaa tgctgatgga
    70861 gcacattgca gagcatttgg tttttgattc gggctatcat cagttgtttt tgattttgcc
    70921 ggcaatagga atattgctaa cagttacatt ctggagactg atgaaaatta aaaatgaacg
    70981 aaggggaatg acggcagtaa tttataacat ttccaagcag tcgagtgttg ttgccaggca
    71041 caaactgttt tcgcagatgg ttagcagtat ttttactgtt ggttttggtg gctcagcagg
    71101 tcttgaagca ccaatcgcat ctaccggttc agctattgga agcaatattg caagcaaatt
    71161 aaaattctct actaaagaaa gaacattgtt gctggcatgt ggtgctgcag caggaatttc
    71221 tgcaattttt aatgcaccta ttgcaggtgt ggtgtttgca ctcgagcttt tgctcattga
    71281 tatgcctgta cctgttgtca taccactatt gatatcaagt gccactgctg cttttttatc
    71341 acgatatttt aatctcggcc agccttttgt tctcataacc gacacatgga attcaagtca
    71401 catactctat taccttgctt tagctgcatt ggcagctata atttctattt actgtattaa
    71461 gatatatttt ttcatcagct cattgttttc aaaaatcacc aatgtatttg taaaagcact
    71521 cattggtgga acattacttg gactgctgat atttttattt ccaccgcttt atggtgaagg
    71581 gtatgacagt gttcagttgt tattgcacaa tcaatctgca gaaattttta aaaacataac
    71641 cttttttgaa gctaatgctt tgtggctacc aattcttgca gcactgctga tgttttttct
    71701 aaaagtagta gcaacatcat ttacggtttt tggtggaggt aatggaggta tgttcgggtc
    71761 atcattattt acaggtgcta tgctgggttt tactttttcg cgagtggtga atattatggg
    71821 ttgggcagag ttgaatgaaa taaattttac agtaattggt atggcatcaa tgcttagtgg
    71881 agtacttcaa acaccgctca cagcaatttt tctgattgca gaaattacag gaggctatgc
    71941 tctttttgtt cctttgatga ttaccacatc aattacttat ttagctacac gatatgtaga
    72001 gccacattct gtttatacca aacaattagc acttaaaggc ggacttatta atctgagtaa
    72061 agacaaacag gtaatcagca aaatgaaatt aagagattat ttagaaaccg atttagaaac
    72121 aattaatatt aatgcaactc ttggtgattt aatcaaggct attcgtaaga gcaggcgcaa
    72181 tatttttcct gtggttgatg ctatgaatca tttacaggga atcatattac ttgatgatgt
    72241 aaagagcatc atgtttgatc agtcacagta tgacaaagga gtcaaagaac tgatgcaaag
    72301 gccaccattg gttttagata tagatgaaga tatggaacgt gttttagaaa aatttgaaga
    72361 gtatgaagcc tggaattttc cggtaacgga aaagggtgtt tatgcaggtt ttgtttctaa
    72421 gtctaagata tttactttct acaggcagct tcttattcag gagtctgacg tgcctatgca
    72481 cgattagcga cagacttaaa agtaaaaaag cccctttgca ggagcttttt tataattttc
    72541 agaagattat tttttcttct tagcagcagc tttcttctta gtagccgctt tcttttttgg
    72601 agccgctttt ttcttagtag ctgctttctt ttttggagct gcttttttct tagttgcagc
    72661 tttctttttt ggagcagcct tcttagcaga aactactttt ttcttagtag ccgctttttt
    72721 cttagtggct ttctttttag tagccgcttt tttcttagtg gctttctttt ttggagctgc
    72781 ttttttctta gttgcagctt tctttttagt agccgctttt ttctttgcag ctttcttttt
    72841 tggagcagct tttttagctt ctaccatttc agcacctgta gtgctttcaa tcattgaatt
    72901 gttcatcatg atgattattt atttattggt tattgatatt gttatactca gagtttgctg
    72961 ataagttaca tttttagttt acgtcaacaa ttttctctgc cttatattca ccgtttaata
    73021 atttcgattt tatttttccg aaagattcaa ttgtatattt tacatcttct aatgtatgaa
    73081 ctgctgtagg tattaaccta agcataataa ttccttttgg tactacagga tatacaacca
    73141 tcgagcagaa aatatcatag ttctcacgca agtcatatat aagttttgta gcttcaggaa
    73201 tagttccatt aagataaacc ggtgtcaccg gtgattgtgt ttttccaata tcaaatcctg
    73261 cttgcttgag tccgttctgc agggcattaa caattttcca aagattgttt tttagctgtg
    73321 gctgattttt tagtagctca agtcttttaa ttgcaccttc tactaacggc ataggcaggc
    73381 tttttgcaaa aatctgcgaa cgcatgttgt atttaaaata ttctattatc tgtgcatcgc
    73441 tggcaataaa gccaccaatg agtgccattg attttgcaaa agttccaaaa taaatatcta
    73501 ctccatcctg aacaccttgc tcttctcctg ttccggcacc tgtttttccc atggtaccaa
    73561 aaccgtgtgc atcatcaaca aaaagtctga agttgaactt acttttcagg gctacaattt
    73621 cttttaactt accctgatcg ccactcatgc caaaaacacc ttctgtaatt actaatatag
    73681 caccaccggt ttcttctgca agtttggtag cacgctcaag ttgcttaact aggttgtcaa
    73741 tgtcgttatg tggataaaca aaacgttttc ccatgtgcag gcgaacacca tcaatgatgc
    73801 aggcatgcga ttcggaatca tatactatta tatcatgacg gtcaactaag cagtcaatag
    73861 cactaaccat tccctgatat ccgtagttta ataatatagc gtcttctttt tgttcgaaag
    73921 cggcaagttg ttgttctaat aatgtatgtc tgtcggtttc gccactcatc atgcgggcac
    73981 ccattggtgt tcctaatccg taatgcaccg atgcttcgcc atcaattttg cgaacttccg
    74041 gatgattggc caatccaaga taattattta aactccaaac taacttttct ttactgcgaa
    74101 aaatcattct gttcgaaatc tcaccttcaa gttttggaaa agtaaaatag ccgtgcgatt
    74161 ctttggcatg catacctaac gggccacggt ttttactaat cttgtcaaac aaatctgtca
    74221 taaaaatgct aaaaattttt ttcgcttttg ctttttaaaa ttgtttccga aatatcacca
    74281 caattttatt tttgataacc atgctactta atatttttca acagaaaaat gttaactgct
    74341 tttttgaata taagaaaagt ttttttgaaa aaaaagtaat cgaagaaaaa aaataaaaaa
    74401 ctttttgttt caatttttca gaggagaaaa taaaagttgt tgttgtaatc attaaaaaaa
    74461 atatttttaa taagcattga ttttttatag tgatgcgcaa aatgaaatgg agtgtgtttg
    74521 ttaatatgtc aacaacctga gcacaaaaaa aattcttaaa atggtaattg tgtgcaaaag
    74581 ttttgatcaa aatctgtgtt aatgcaaaca aaaaaccaat tcagtttttg tgttgatgag
    74641 taaaaatatt ttacgattaa attttcggaa aatgcaagat tgtttttagg taaatgatta
    74701 ctgaaagtgt gtttcatgat agttctaaaa acagatgtag tgtatttata aaacaaaaaa
    74761 gccgcttcgt tatcggaagc ggctgtatgc tatttgtttt tttaagaacc tctttgtttt
    74821 tctttatgtt ttgtaatctg tcgctttaat gcttctacag atttatcggc agcctgctca
    74881 aaggtggcgc attgttcgct agcaaaaagt actttgcccg gtgtgctgat ttttatttcg
    74941 gcaattttat tttcctgcgt cacgttttta tctaaacgta aaaatacttc accgtctata
    75001 atgtgctcgt aaaactgttt tagtttgtca accttttctt ctacaaaacg aatcaggtct
    75061 ttgtctgcat caaaatggat ggactgcact ttaatcttca tactctattt tgtttttagt
    75121 acaaaaccca aacattgccc ttgtgccgga gttttgtggt tttaaatatc agatgggcat
    75181 gtgtttattc aattataaaa agtaaatata cgaaatcagg ccttaggatg tgcctgtttg
    75241 tagatatttt tcagtttatc tattgtgttg tgtgtataga cctgagtagc ggcaaggcta
    75301 ctatgtccta aaatttcttt gatggcattt atctgcgcac cgctgttgag catttctgtt
    75361 gcaaaagtgt ggcgcaaaac atgcgggctt ctttttttgt tggtggtcac ctgactgagc
    75421 attacattaa ctaccttata aacgaatttt gggtatagtt tgttaccatc tcctttaata
    75481 aaaaagtatt ccgacttgat tttttcatca atgatgttgt ttcgttcatt tagatataca
    75541 actatagctt ctttcatttc cggtgtgatg ggaatgatgc gctctttatt tcttttaccc
    75601 aaaactttta aagtaagatt gtagaggtca acgtttttat cttttagtcc tatcaattcg
    75661 gcaagtcgca tacctgtctg ataaaacata tttataataa gcttgtcgcg aataccttca
    75721 aacccttctt taaatgcaac accatcgagt aactgatcca ttttttggcg gtcaacaatt
    75781 acaggtagtt ttttggcagt ttttggcgaa acaattttca gcattggatt ttggctgatg
    75841 gttttttcgc gcatcaaaaa tttgaaataa gttttcagtg atgatatttt ccggtttacg
    75901 gttcgtgaat ctattttccg gtctaataaa gaaactatcc aactgcgaat aaaaaagtgg
    75961 gtaatatctt cgggtttctg agcctgatag gtctggccca taaaatcaaa aaactgctta
    76021 aggtcggtac cataagcagt taatgtatgt tgcgaaaaac gtttttcgta ttgaagaaat
    76081 cgaagaaact ttgcgtaagg catattattg tttatgccaa atataaaggc atattcaata
    76141 agtgcagttc ataacaaggc ttagaaaaga tgaattataa aaagcctttt gttaagaatt
    76201 acattgcgcc ttcggcctct aagcgctgaa gttttgtgcg gtgtattgct ttttttactt
    76261 ctgttctgcg ggctactgat ggtttttcaa atgcctgacg tgaacgcagt tgttttacaa
    76321 cacctgtttt ttcaaacttc tttttaaact tctttaaagc cttatccaaa ggctcattgt
    76381 ctttaatttg aacaataatc atattataaa tacaaaatgt tttgggagcg caaaggtaag
    76441 taaataattg gcataataat caatacaata gaaaattttt aagaaagtac tcacctgcct
    76501 gtatttgacc ttaactattg attattaata tgatggagta tatttctttt ctgtttttat
    76561 gacaaagcag gtggaaatta catcaaatcc ttgccaatat ccttcctgta aaaggctttt
    76621 tcgtaggtaa tttctgtcaa ttgcccatag actttttcgc gggcttctgc cagattttta
    76681 cccaatgcat ttacagcaag tacacgtccg ccattggtta gtactttgcc atcatgtatg
    76741 atggtgcctg catgaaacac gtttacccct ttaacttttt caagccctga aataattttt
    76801 cctttttcat aagtttctgg gtatccaccc gaaaccatca taacagtaaa cgatgttctg
    76861 tcatctgttt ttagctgaac ctcgtgaagt tttttatttg ctacactatt catcaactca
    76921 ataaaatcag tttttattct cggaaaaacg gcttctgttt ccgggtcgcc catgcggcag
    76981 ttgtattcaa taacaaaggg attgctatta acattcatca gtccgataaa taaaaatccg
    77041 gtgtatgaaa tgttttcatg ctttaatcca tttatggttg gtctgataat tttatcttct
    77101 actttttgca taaaagcagc atctgcaaac ggaaccggtg atactgatcc cattccacct
    77161 gtattgagtc cggtgtcatt gtcgccaatg cgtttataat cttttgcttc cggcagcaga
    77221 caataattgt tgccatcagt caaaacaaaa acagagagtt caataccttt cagaaattct
    77281 tctatcacta ctttgtttcc tgcctttcca aaaggttgat tgatgagaag atgatgcaat
    77341 tgctctcttg cctcagtttc tgtttcgcaa ataataacac ctttgccggc agccaaacca
    77401 tcagccttta gtacgtaggg tgcagatagt gttttcagaa aatcataacc ctgctgcagt
    77461 tgcgatgatt caaaagtttc ataacgtgct gtaggaatat tattccgcaa cataaaagct
    77521 ttagaaaaat ctttactgcc ttcaagtctg gcaccggaac tttccggacc aataacagga
    77581 acagatttta actgactgtc attaagaaaa aaatcatgga tgccattaac caaaggctct
    77641 tcggggccca caattaccat atcaattttc ttttcaagca cagttttttt gatgtggtcg
    77701 aagtcattca cgctgcctga aatatttgtt cccaactgta atgtgcctgc attacccggc
    77761 ataatatata agtgtttaag caatgcactc tcactcaatt tctgcgcaaa tgcatgttcg
    77821 cgtccacctg atccaagcag taaaatattc atgctaattt tctttctgca atgataaata
    77881 aatatggcgt gccgtagcat tttgtttgtt aattgacagc ccgaatacag tgctgaacgg
    77941 acatgtttct atattaaaac aggaaggtgc caataactga tttttttact ttatttttat
    78001 gccgtagaaa attctgatta gaaaaaggaa aaactatatt ataaaagcaa acaacaactg
    78061 atgaatagcc tttttaataa acaagataat gatagaataa tagaacgaat agcagcactt
    78121 cagcctggac aaagagctct ttggggaact atgtcggtag aacagatgtt gctacattgc
    78181 cagcaaccat ttaaagtggt gtttgaagag ttgaaactta aacgcagctt agtggccaaa
    78241 ctatttggag gtatagtaaa aaagtcggtc atggatgata aactatttaa agaaaacctg
    78301 cctacggcag cagaatttaa aaccaatcat ctgagtccgc agtttgaaac agaaaagaaa
    78361 aatctgatag cttatattca gaaatttcag tctgcaggac caaaaggagt aacaaaacta
    78421 cctcatcctt tttttggaaa aatgacagat gcagagtggg ataagctcca atgcaaacac
    78481 ctcgatcacc atttaagaca atttggcgta tgaaatttag gttcgttcct gtggttttta
    78541 taatatcttt gcttatgatg tcgtgcgaaa atgatataga gcaagtgaaa ttagttacag
    78601 gcaacgttgt tgaacctatt gaaagtgcta aggggcttga aattttgtac agcgactcgg
    78661 gcaaagtaaa agtgcgcatt gcagccggtg aaatgaatcg ctatctgcaa cccaagccca
    78721 tcactgaaat gcctaaaggt gttaagattg atttttttga tgacaacctg aaggtgactt
    78781 ccacactgac aagcgactat gccgtgcgtg atgattttaa taaaaacatg gaagcaaaaa
    78841 aaaatgtgga ggtggtgaat gttaaaggag aaaaattaat gactgaatat ttaaagtggg
    78901 atgaaaaaac cggacttatt tcctcaccgg aatttgttaa gataattact gccgatgaaa
    78961 ttatttacgg caacgggttt gagtcgaacc aggatttcag ccgctacaaa atatttaata
    79021 ttaaaggcat tattaacctg aagaaaaatg aaaatactga gaatacttga gatatcgtgg
    79081 ctggtaatag ccataggttc attatgtgcc ggaatctata aactgatttc agcaggactc
    79141 aatcaggctg tatttatttt tgctatctct attatagcgt ttgtcttttt tatgataaga
    79201 cgaaaacaaa gaattgccat ggagaagcag aatgcttcac gataattact tttgcagcac
    79261 taaatagatt taattcctgt taatgatttc cgactttctt cctgccatac ttgcatcact
    79321 tgcttttgtt gcattttatt cggggataga aattgcctac ctgtcaagca ataaatttaa
    79381 aatagagctc gacagcaagc aaggcaaatg gagtggcaaa attttatctt atttcagcaa
    79441 gtcgccttca cgttttatct gcactatact tgtagggctt aatatttcat tggtgattta
    79501 cggtaccaat atgagcggac tgctcaatcc ttatctgcaa caatggctac cacagcaact
    79561 caatagcgaa atagggcgat tgataatcga aacattaata actacttttt ttctgctttt
    79621 ggcaggagag tttttaccta aaatattatt cagcataaac ccaaacgaca cattaaattt
    79681 ttttagtgga tttatctgga tcagctatat tcttcttttc cctttggttt ggttaatttt
    79741 aaaacttgcc cattttctgt tgcgtaatgt ttttaaagta gattttgttg aagaaacttc
    79801 cggcttcggc agggttgact tagataattt tattgaagag ataagcgaaa ataccggagg
    79861 taagagcgag cacagtaaag aaattcagat gtttcagaat gcgctcgatt tcgatagtct
    79921 gaaagtgcgt cagtgtatga taccacgcaa tgaaattatt tcgctcagca gcaaggagtc
    79981 agtagagaca ttaagaaaga aatttgttga aacgggatta tctaaaataa tgatttatca
    80041 ggacaccaaa gataacatca taggttttgt acacagctat gaaatgttta agaatcccga
    80101 gagcattatg tcagtactgt tgcctgtatc tatttttccg gagacattgc ccgcacgtga
    80161 gttgctcaca catcttactg caaaccatcg tagtgttgcc attgttgttg atgaatatgg
    80221 tacaacttca ggcatggtaa ctattgaaga tattattgaa gaaatttttg gagaaattga
    80281 tgatgagcat gacattgatg tttatctgga acagaaaatt tcggaaacag aatttttatt
    80341 tgccggacga cttgaggttg attatctgaa tcagaaatac aatcttgaac tacccgagtc
    80401 ggaattatat acaacttttg cagggctaat ttttaatcat cttgaagaca ttccgcatgt
    80461 aaaagagcag tttaccattg gcagttacaa aataacagta gagcaggtga gcggaaataa
    80521 aattgagcag gtaagattag tgattttagt taatgccgaa aactaaattt cctcttaata
    80581 ggctcgggca ttgattccct gcatgtagtt aatgaaagct ttgctcacca ctttattgcc
    80641 accgggtgtt ggatagtttc ctgtaaaata ccaatcgcct ttattatgcg ggcaggcaat
    80701 gtgcaggtct tctattttct gaaaaattat ttctacatca gctttgcagt cttcgggttt
    80761 taacattgat gtgatttttg ccgaaagatg ctcaggcgag aaaggcttgt ataaatcacg
    80821 cacataattt ttcatttcgt ttacacccaa ttcaagttgc tgcttgcaat gcatgtaggt
    80881 ttcttcaata atatgctcca tattattttc ttcaagcaat gcaatggctg ctttgaatgc
    80941 acacaggtca ccgatttttg ccatgtcaat tccataacaa tcggggtagc gtatctgtgg
    81001 tgctgacgaa acaattataa ttttcttcgg acctaaacga tcgagcattc tgatgataga
    81061 attttttaat gttgtgccac gcacaatgct atcgtctata accactaaat tgtcaatgcc
    81121 ttttctgatg gttccgtaag taatatcata aacgtgtgtc accatatcgc tgcggtcgtc
    81181 atcgttggtg atgaaggtgc gcagcttggc atctttaatc gctatttttt caaacctggg
    81241 tttaatggaa agaatttcat tgagcttttc gtcagaaatt tcttttccta aagattttat
    81301 ttttttcttt tttacctcac gcaaatagtc ttccattccc tgcatcatgc catagaaggc
    81361 aacttctgcc gtattgggta taaaggagaa aacagtgttt cttaaatcgt tgtcaacagc
    81421 aggcagaata cttttgcaaa gcaggcgtcc taattctttt ctttcctgat aaatttctgc
    81481 atcgctgcct cttgaaaaat atattctttc aaacgagcat gatttgcgtt caacaggttc
    81541 ttttatacaa ggcattgaaa tgttcccgtt ttttttcatt atcagtgcat gaccgggttt
    81601 tatttcggta atgtcgctga acgataaatt aaatgctgtt tgtatggcag gacgctcgct
    81661 ggctacaact accacttcat tatcggcata catatatgca ggacgaatgc ctgcaggatc
    81721 gcgcaacaca aaagcatcac catgacccat catgccgcag ataacatagc cgccatccca
    81781 tttttttgaa gactgtttta gaatgctttc cacatcaaga tgctcggcaa tgagcggtga
    81841 gatatcttgt ttagaaagcc ctttctcttt aaatgaatag tataatcttt cgttttcttc
    81901 gtcaaggtag tgaccgatgt tttccattac ggtaaccgtg tcggcttttt cttttggatg
    81961 ctgaccaata cgaatcagat tttcaaacaa ctcatcaaca ttggtcatat taaaatttcc
    82021 ggccacaact aagttgcgtg tcatccagtt gttttgtcgc agaaaaggat ggcaggcttc
    82081 aatgccattg ccaccatagg tgccatagcg caaatgcccc atccacactt cgccactgaa
    82141 ggctagatgt tttttcatcc atgcagtatc attcagcttc gatacatcat cattaacatc
    82201 ggctttaaac ttattgtata ttctggaaaa aatatcctga atagcatgtt tatcgttgga
    82261 acggtagcgg ctgatgtagc gtgttccggg ttcggcatca aattttatgt tggcaacacc
    82321 ggctccatcc tgtccacggt tgtgttgctt ctccatcagc agatagagtt tgtttaatgc
    82381 ataaaaagga gtaccgtatt ttttatggta atagtcaagg ggctttagca gccttatcat
    82441 ggctacgcca cattcgtgat gaaggatatc tgacatgaag ctacaaagat atagcaaaaa
    82501 ggagtacacg acaatagcat ttcataaaat atcagctgtg ccccgtaaaa agcaatacag
    82561 agtttatgtt taactattcc ggggaggcgt ggtgctagga aataattaat caatagcgcc
    82621 ctgaataatt tcatctttta ggtaacgctg cccctctgac agtgtgattt aaacaacatt
    82681 ttattttctt catccacaat tttcatcagt gacattttag aaaattttaa cattctaaac
    82741 ttttcaactt tctaaacctt cttttgacaa ttgcgataat ccgcacaatg agttgcgaat
    82801 attttgtata tttgtttcat aatgaataaa cttgtgaatt gtgagtgttg ataatataaa
    82861 tgttatcagc cattttaacg ctccgaacaa actgtactgc taattcaaaa cgacataccg
    82921 tgaataaaat ctttgccata ttattactaa ccagtggaag ttgcatcggg caaaaccttg
    82981 ttcccaatga cggctttgaa attcacacat cttgcccata tgttggaaac tcattcagta
    83041 cgttatccta ttggttcaac ccatccttaa atcttggttg gggttctaca ccagattact
    83101 acaacggatg ttcaacttta ttaaatgatg ttgacgtccc atcaaattgg gctggttacc
    83161 aatttgcacg aaatggtgta gcatatgttg gaatagtgct ttggcataat caacttactt
    83221 tcagagaata ttttagtgtg cagttaatat caccattggt tgcaggcact tcttatcatt
    83281 ttgaaatgta tttcaatttg gcaaaccaat tcaaatattc ttgcagtaac ataggagtat
    83341 atttttcaga tactttatat accagcacta tcaactatga ccccttacct tttaatcccc
    83401 aaataaataa tataaacagc aattatcccg atacaattaa ttggtcaatg gttagcggca
    83461 attatgttgc tcatggagga gaaaattata ttatcattgg taattttcaa aatgactcta
    83521 acagttatcc aatattagta gattccaacg catttgaatc aggcacttat gtttttattg
    83581 atgatgtttc cttaactgtc tatacaggaa tcactgagca agagaaaata gaggttatta
    83641 aagtatatcc aactctgttt gcagataaat tatatttatc atgcgaagtc aacgaaagtt
    83701 gttgtgtaat actttatgac gaaaccttta gaaaaattat tcaactccca ttcacgaaaa
    83761 atgcttcact aaacacaggg catcttgcaa agggaatgta tttttacgaa gtacggtgtg
    83821 ggagtagttt atgcaagaaa ggaaagttgg tgaaagattg aaattataca gcttcgtgga
    83881 cacagcaaaa catgcgcata acagtaagat taaaagaaat aaaaaaaaga aggtccgata
    83941 gttatcggac tttgagaggt aaacaaaatt gtctgaacca tgattaaaag gattagaaga
    84001 ttacttgatt ttaaacaaaa gatttgaaat gaagcatgag gatgttacca ggcgaataat
    84061 tgcctcggca atgaaagttc attccacgct gggaaatggt ttccaggaag taatttatca
    84121 aagagcgatg gagttggaaa tgccatttga aaatttgttt tttaaaagag aaatggaaat
    84181 gcctgtacat taccgtaatg aaataattgg tacaagaaga gtagatttct ttgttgaaga
    84241 aattgtgatg gttgagttga aagcaattat taaattggaa gatgtgcatt tggcacaggc
    84301 aatgaattat cttgaggcat ataacatgga aataggctta ttaataaact ttggaagtaa
    84361 aagtttagag tttaaaagag ttcacaataa taaacccatc ctaaaatcaa attaatcagg
    84421 caaatcatgg ttcagacaaa cagtgttgct aatacaaaca gttcgacaat gagaaaacaa
    84481 atctgcgtta tcattttgat gttgacttgc agaatttgtg atgcacagaa tcttgtacct
    84541 aatggagatt ttgagcagta ttcgttatgt ccagactttc cggctaacct tgctttggcc
    84601 ttattttggt tcaatccttc aaccaatgtc caaaatgttt ctggaacctc tgattactat
    84661 aatgcatgcg ccaacgactt ttacccgatg aatgtacctg gtagtttcac tggttatcag
    84721 gttcctcaca gtgattctgc ttacgcagga atcttattat ttgaacattt aggagttaat
    84781 tatcgcgaat atatcgaagt tcaaattact tcaactttaa caagtaatac ctgttatcat
    84841 tttgaaatgt atatcagtct aggtgatatt ggtaaatata caactgatga tattggaatt
    84901 tatttttcag atactgttgt ttctaatatt aataattatg ctccattacc atttattcca
    84961 caaattaaca atgtaacagg aaattatccc gatacaacaa actggctttt agtacaggga
    85021 gattatttag ctacaggcaa tgaaaattat ttaattatcg gaaattttaa agatgacttt
    85081 tctattgata ctttgcttta taattcacaa ggttataatg catcttatat atacatagat
    85141 gatgtttccc tcattctcac tccttgcacg ggtattgaag aacaaaattt aaattcaaat
    85201 ataaaaatct atccaaatcc tctatcagat atattaaata ttaaagcgtg ggggttttca
    85261 gaaattatcc tttatgatgt tgcagcaaga aaattattgc aacaaaaatt tacaaactca
    85321 acaactgtaa acactgaaca acttgcgaag ggtttatatc tttacgaggt gcgaaacaaa
    85381 aatggtttgt gcaagaaagg aaaagttgtg aaagaatgaa gtgggacaac ttctcgcgag
    85441 agaaaaacgg ctgataacat ctaagactct tgtaggcgcg atcgtcagtt ccgctcgtgc
    85501 catttagctc ttacgacttc cagtcgcatt caaaatttgc tatgtattta tgtggtttca
    85561 tagtctcacc gatttttcat cgggggacta tgaaacgggg attgtaattt cacctttcag
    85621 gttgtgcagc ccctgacagt gtgatttaaa tttcaacctt caaaactttc aaactttcca
    85681 aaccttcgaa acatactttg aaataataaa aaaacattga agagttaata cggtttgaat
    85741 ccgtttcata gtcacagcga aggggcggga gactatgaaa ctgcaaaagt gttttgtttg
    85801 ttaccgttgc cactgcaaat attgctgtgc gaattagcct tgatttgttg actataaaag
    85861 ttaatcgtta gttttgagca gttgtgcaac aggcgcagaa gtttgttgtc aataattatt
    85921 tgcatgacac aacttctttt tattgaaact tttgtattgg gtgcttctgc cattgcagcc
    85981 atttatcatt tagtgttgta tatacaacag cgcgataagt ttttgctttt ttacagcata
    86041 catttactta ccctctctgc ctacatttta tttaaactgt taagcagtaa ctacaatccg
    86101 tttttaccaa ccggcaattt gtggaattat ataacagaag aaacgttgca aggtgcaatg
    86161 gttactgtgt atgtaatatt tgctgctatt acacttgatg ttgctttcag cagaagcaat
    86221 gcacgtacgg ttatgtacat cttttttggt ttctgtattc tattttacag ttatcaatta
    86281 tacaatgctt ttgaatatgg cccgggagta aaatcaattc aagcctatat ggtaacaagg
    86341 ttatcgcttg taggcatagc tgccatagcc ttaatttttg catggaccat acgtacttcc
    86401 attttccatc gcactatcat tatcgggtct cttgtttatg atttctcatg gttggttagc
    86461 attatttctt tgtcaacaca aactactatt ttgggtttaa atggaataga gatttacctg
    86521 ttaggatgcc tgttagacat tattattttt tcgagtgctt taggatatag ggtaaaaaca
    86581 atggcagaag aaaaaaatga attggtaaga aaggaagcag aagcccgttt ggccatagaa
    86641 aaaaccagaa caggaattgc catgaaccta catgacgaca ttggttccgt actgagcagc
    86701 atgagtgtgt atggcgaagc agccaaacgt gcgctcaaag aaaataatgt ggctaaggtt
    86761 gatgttttga tagacaagat tggagttaat gcccgcgaaa ccatggacaa catgagtgat
    86821 attgtatgga caataaaccc tgtcaacgat aatggattta aactttttaa tagaatggag
    86881 tcatttgcct catccgtttt aaatgcaaaa gaaattgaac tgaagtttga agtgggtaaa
    86941 gaattatatg aaaaaggttt tttaatgccg gtaagacaaa atgtattttt tatttttaag
    87001 gaaattatta caaactgtgt aaaacattcg ggtgcaacaa atgttgaagc tatatttact
    87061 ttaaatggca gtaacctgtt gcttttagtt agcgataatg gaaaaggttt cgacacaaaa
    87121 aacaatcatc ttaaaaacgc atcaggcggc aatggtttaa aaaacataaa agagcgtgca
    87181 tcagttttaa atggaactat agaagtaaaa agctcggcaa acggaacaga aaccaaactg
    87241 gagttcccgc taacataaaa cagcctatgc ccgaatttgg ggatgacacc atatattaat
    87301 tgaacctatt tttgtttcaa taaaatgaac cattaaataa aacacaacat ggcagtacgt
    87361 gtaaccatat ttgacgataa taaaagattg cttgattcgc tatcggtgct aattgatggc
    87421 agcccgggtt ttcagatggc aggcatattt gaaaactgca ataaccttat tagcgatata
    87481 gaaagaacac aacctgatgt tattttaatg gatattgaaa tgccgggcat taacggaatt
    87541 gaagcggtaa aaatggtgaa gcaccgtttc ccgaaaatga atgtgatgat gcaaacagct
    87601 tttgaaaacg atgaaaatgt ttttgcagct atatgtgcag gtgcatcggg ttatatttta
    87661 aaaaacacac cgccctcaaa aattttagaa tgcataatag aagtttacca gggtggcagc
    87721 cctatgagcc caagtgtggc gcgtaaggtg ctgggctttt tgcaacaacc caagcccgat
    87781 gagaaaaaag aaactactgt agaaaactac aacctttctg cacgcgaaaa agaaatattg
    87841 gcgtgcttag taaaaggcat gagttataaa atgattgccg agcaatgcaa cattacctac
    87901 gaaaccgtgc gctcgcacat gaaaaacatt tacgaaaaac tgcacgtggc cagcatgaca
    87961 gaagctgtag ccaaagccat taaccagaaa ttagtttcgt agtaaactaa ctcaactctt
    88021 tgtaaaagcg cactccaaaa cagtgtgctt ttttattatc cccgaattag gggattgatt
    88081 tcggccttgc cacaacccat ttttgctgca tcaaacaagg tcaaactata taaataaaat
    88141 caaatgaaaa aattgctatt caccttaatg ctgctaagca gctttgcatt acaagcacaa
    88201 aattttactt acaaccgatt ttttaacgga ggcgacaatt cagcaataac cctgggcgaa
    88261 ttagccaaag aagtaattgt acagcctgat ggaaaagtta taagttgcgg ttactcttat
    88321 gattttgcat gcaactgctt tcacattgtt atgttcagag tagatgtttg cggtaacatt
    88381 gattctacct ttggcacaaa cggcacagta tcttacactt ttgaggaccg caacacaggt
    88441 ttagattttc taatgcaggc aaatggaaaa atagtagtag tgggcacgca ggcaccaagt
    88501 aatgccagca gtcagcataa cccttatata gcacgctttt taaacaacgg ccagcccgat
    88561 accacctttg gaaccatggg tacaactaaa gtaattgtta accctgcttg ttgggaattt
    88621 acccacgtta ccgaagtggc agatgggaag tatctcgctc aaaacggtaa ttacatcatg
    88681 cgctttgaca gcactggtgc tgttgattta tcatttggaa atagcggtgt tataaattat
    88741 accattccat caaatgttcc gcttgtgagc tcctggagga gtgtgcaacg cagcagcgat
    88801 aaaaaaataa tttcggtaac tgctgcatgg ttaggtagcg gcaatgatat gcgcccattg
    88861 tttatgtgta ccgataccct tgggcagtta gattccactt ttggtaccaa tggcttttta
    88921 cttgataata acgttatctt acctaactat cagtcttgcc atattgtgtt acaaaacgat
    88981 gataaaattc tgtctgcaca ccacaacgcg gcagaaactg aaattacggt tgcgcggtac
    89041 aacaccaatg gttctttaga cacaacttat ggtaccaacg gatatgtaaa ctatacagcg
    89101 cccacctcac cattcagagc aagagatgta aatatactta gcaataacaa tttgttggtt
    89161 atggccgatg ccgcagggct tgaccgtcaa ataaacactt ttgatacaaa cggggtaatg
    89221 accaacagcg ttactttttt tggaaacaat aattataatg tatcaggcag cagtgtgcca
    89281 ttggtattgc atgttgaaaa cgatgatgaa atatacatag taagccaaac cggtggtact
    89341 agtggcatgt ggtatatttc aagcctaacc acaacccctg ctccaactat ttcacaaaac
    89401 tttacatcgc taagtgcaaa ttacaattta acaggcacta cttaccaatg gtatttgaat
    89461 ggcaatatta ttaatggcgc aacggatagc acttatacat ttacacaaaa cggggtttat
    89521 agtgttacag taaccattgc ctcaggctgc gattattcgt attcatacac ggtaaccaac
    89581 accggaattg ctaatgctgc taaccttaac aacattaagg tttcgcccaa tccgtttaac
    89641 gactatataa acattagcaa tacaaccggc cagcttacaa cccttacttt atttgatgcc
    89701 gcaggccgta cagtaatttt agaaaccacc catgagcctg attataaaaa gcaactacaa
    89761 caactgccaa aaggcatgta ttttctaacg attgaatccg gcagccaatc caagcatatc
    89821 aagcttctga aacactaaat acaaccctgc aacagcgcac ggccgcaagt ccgtgcgctg
    89881 ttttaatatc cccgaattat gggattgacc aagcctacct cccaaaatat ttttgttacc
    89941 tcaactgccg gcaattatgc atgtaaaaat atttagtgta cttatatgta ttgtactctt
    90001 aaacacaaac agtgcctttg cacaaggcgt gggaattaac aatccaacgc ccgatgccac
    90061 agccctgctt gatttaacca gcactaccaa aggtttgctc ataccacgga tgatgacttt
    90121 tcagcggcta tccatacccg cacctgccaa tggcttaatg gtttacgaca ttacattgaa
    90181 tgctttctat tattcggaca atggcatttg gaaacccatg gataatagct ggcagctaaa
    90241 tgggaataca gctaccaatc ctgttactga ctttattgga acaacggatt ttacgccttt
    90301 ggtgtttcgt actaacggtt ttgaggcgat gcggattaca caagatggca gaattggcat
    90361 caaccagcca ttttctccag catttaatgc cattcttgat atacaaagta acgataaagg
    90421 catacttata cctcgcatgt cttcacccct gcgcagtaat atttcttctc ctcctgacgg
    90481 cctattggta tatgacagtg gttcaaatga attttattat tctatatctt ctgtttggat
    90541 gagattgctt tctaacaata cggggtggac cactagagga aatgctttca ccaatccagc
    90601 cactgatttt ataggtacaa cagataatac cgatttagtg tttggtacat ttggtggagc
    90661 acgagggtgt ataacttcag ccggtgattg gggcataggt accatttttc cttctgcaaa
    90721 gctgcatgtg cagggcagtt accggttaga agatgggaca caaggcctgg gaaaattatt
    90781 aatgagtgat gctacaggca atgcaagctg gaaaacattt gaagacctta ttaccggcaa
    90841 ggtagctaat gggctttact ttaatactat acaacaaagg atacttattg gcggtgcatt
    90901 aaccgaaaac accactatta caaattccgc ttttgatttt acttatgacc ttaccggtgc
    90961 cggtgacttt caggtaacag aaagttccaa tccaatacct tctctttttg tttcaggcaa
    91021 caagggaggc gttaacgatg gatatgtagg cataggtact gccacaccgt ttggcaggtt
    91081 tcatatcaat aatacagtgg ttaccacagt aaacacacaa aacacctatc acttattaat
    91141 tgggaacggt aatacaaacc ctgatttaac ttttggagca agtgcaacat ttgcactcgc
    91201 acaaagttgg aacaataaac ccctacaaat taacccacta ggcacctttg ttggcataaa
    91261 tcatatttca gcagcaccca tacaaaactt agacatcaat ggccggcttg ccgtgcaaaa
    91321 cggagttatt caaaaaggcg cagcaacagc aatattggct actgacttgg gtttgtacca
    91381 gcaaatagct gggggcacca tacgcatatc caataatgcc ggccccataa agttttatac
    91441 cgaccagggt ggtgcaagtt tgggtggcac caataacctg gtaagcattg acaacagtaa
    91501 tggcggaggg gtaaaaatcc acagcgaaat tagtggatcc gggcaagccc tggtacctgc
    91561 agcacgtgcg gctttagatg tacaaagtac caccaaaggt gttttatttc cacgcatgac
    91621 tacggcacag cgcaatgcca tactgccgct taccgcagcc gatgaaggct taatgattta
    91681 caatttaacg gagcattgca taaactggtg ggacacccgc cctaacagtg ccggtgtccc
    91741 cggcttttgg aacagccaat gccagccttg tatggattac tttgaataca ctgcaagttc
    91801 taacggcaac aattttaata cgcaagcagg ttcgcccgtt gggcctaaaa cattttgtgt
    91861 tacggttaag gcaggtgtta ccttgggcgc aagcgctatg agtggcgatg ctttaaactt
    91921 tggcgcttta cctgcaggtt ccaaagtaat attacacaac tacggccaaa tacgtggagg
    91981 tggtggcaaa ggcggcaatg gtggctcaga aagggatgtt actgtttgcg gggttgatgc
    92041 accggcaaca gcagggtccg atggtgggga tgctattgtt acttcggccg gggtgcaggt
    92101 tacggtttat aactacggca ccattgcctc aggcggtggc ggtggcggtg gcggcagcgg
    92161 agggtgccgc agcaatggtg gcagtggtgg gggtggtgcc ggcataccag ccggtggtgg
    92221 tgggttaagt cctaacacag gtggcagagc atccggtggc ctttgcaatg tatgtactct
    92281 tgcgcctgct tcattggctg gtgctaatgg aaccataacc aatggtggcc ttggcacttg
    92341 ctcacaagga aatgcaggtg gtggttgctc ttcgcttggt tttaatggag gttgtggtgg
    92401 caatggtggc ggccccggtg ttaatggaaa taccggagtg ggcctttcct gcggcacagc
    92461 catagccgca gccggtggca atgccggctt tgcattacaa ggcaatggca gtggcagttt
    92521 tattgtaaat gttacaggga catttttggg tatcgtatct ccataattac tcacacttaa
    92581 gtagtatttt tttcatcctt aaaccatttg atatttgcct gtaacaaaaa tttaccgttg
    92641 gataaaataa catccactta ataaattaaa ctggcttaac ccttttgtca gcctttaaat
    92701 tgatgcataa atgaaaaaga aaaaatataa caccaagttg aatctaacat gcattctcat
    92761 ttccatgttg atggtgattt tggttttcaa tgaatcacct gctcagcttt taaccaataa
    92821 taatgtttta atttccaact caatacagat tacagttaag ggtactgtgc aaaataattt
    92881 tggaacggtg atttcaaatt cgggcacaat tgatttaact ggaaactggg taaataattc
    92941 aggaaacaat ggatttggga tttcgcaggg aaccgtcatt atgaacggag taaatcagtc
    93001 tatacaagga actgaccaaa cggtgtttaa taatcttagc ctaattaatg gaacaaaaac
    93061 aattgattta aatacaaccg ttggtggtgc aaatgcaaca cccacaggag tattaaactg
    93121 taacaatgct gttctcgatt taaattcaaa cacgctaacg gtaaacaatc ctattggcac
    93181 ggcaattacc acttccacag gttatattct tagtgaagat gttgaccaca gtagtaaagt
    93241 tacctggaaa attgcaagca ctacaggtat tcattccatc ccatttggta atgcaggtgg
    93301 agtgcagatt ccttattcat ttaatctcac aactggggtt gcaggtgaag ttactgtttc
    93361 aacgtatgct actgttcctg tgaatactcc ctacccagtg tcaccaacaa tggtgactca
    93421 tgtgcgtgat gcatctggtg ctgataacag cgcaaataca gtggacagat tttggcaggt
    93481 tgacaaaaca ggaaatggaa ccgcagccta tacttttact tatgcagcaa cagaaaatgc
    93541 agcaggtgga aatataaata ttagagcaca atcatggaat acaactaact taggatggga
    93601 tgctgcattg ccaggacagt taaaccctac ggtgcaaagt gttttagtac cgggggtaaa
    93661 tactaacggt gcctgggcac tggctttggc atcaaatcct ttaccaatag agttgctttc
    93721 gtttactgca aaatccaaag acaataaaaa agttgtttgc aactggagca cagctagcga
    93781 aatcaacaat gattttttta cggtgtttcg cagcaaagac ggaataaatt ttgaagaagt
    93841 tagcaaggtg gatggtagcg gaaccacaaa cactcaacac aattattctt ttactgacaa
    93901 caatccatat aaaggtgtat catactatcg cttaaagcaa accgatttta atggggacta
    93961 tactttttca cagattgaaa atgttttaat cactgatggc gatataaagt acttagtgta
    94021 tccaaatcct aacaacggaa atttctctgt taaatttgat gctgaacaaa acgtcaatgc
    94081 ggtattttta attacggatg caacaggacg gttaattcag agcggaagat tagatgattt
    94141 atcgggcaaa cagcaaatta atattgaaaa ggttttgcct ggattttatt ttttgcagat
    94201 aacaaatgga gataactcct acctcgcaaa agtgcaggtg ataaaataat cagccgttaa
    94261 ataaattttt tacttcagca attttcctgc gtgagatttc cacacgggtt tcatcctgaa
    94321 gaataggaag atggtcactg ttcagggctt ttaatttcag gttgcgcagc ccctgacagt
    94381 gtgatttaaa tttcaacact caaaacattc aaaactttcc aaaccttcga aacatacttt
    94441 gaaataataa aaaaacattg aagggttaat acgtttttga atccgtttca tagtcaccgc
    94501 gaaggggcgg gagactatga aactacaaaa acactgccct tgacagtgtg atttaaattt
    94561 caaccttcta aactttacaa ctttccaaac cttcgaaaca tactttgaaa taataaaaaa
    94621 acattgaatg gttaatatgc tttgaatccg tttcatagtc acaacgaagg ggcgggagac
    94681 tatgaaacta caaaagtaaa gattatggaa gaattattaa cgaaaaattc ctactattgt
    94741 aaacccaatt caggactaaa aataaattgt aaacttatat caggattaat taattaaact
    94801 gtaaactttt ttcaggacga gtcagtgctg catttcgctc aaatcatccc acaaccaaaa
    94861 aatgcaccac acaatttttc tcctccgcac cctatccaca tgcccaatct cattaattga
    94921 aattattccc ttatcaccgc taccttccta ctcacccttt tcccatcact cgttatcaca
    94981 caattataaa ccacattgca aaatgtaagc atctttattc ttttactaac tttaatacga
    95041 tagatatttt gctttcccaa acaacgctgc aaaagtatat cccagaagca agcgatgaaa
    95101 tatttaattg ttttgcattt tcgagaggag aagttgaaaa agtacgaaca agatttccgc
    95161 ttgaatcatg taatttaatt attcctgaaa ctttttgcaa cggaaactga atagaataaa
    95221 ttccattgga aatagtagga agtatctgga tcggcactaa agaaatttga ttctcaattc
    95281 cagtgcatgc tgaaagtatt ctataacccg ttactataca gccaccactt ccggtggccg
    95341 aaaaccaata actgcccgga gcgtttactg ttattgatga agtagtagct cctgtattcc
    95401 aaaggtaatt ggttaatccg ttaggtgcat ttagcataag agagcttcct gcacataata
    95461 cagagtcatt gcctatattt acaaaacctg gattccctgt aaatgtctta aacccctcaa
    95521 tatttggtgc tgaataaatg gtatcatgac ttgcccaaat agaggtcata taaacatttc
    95581 cttgtagggc ttgcgtaaac caacaagtgg cgatgccggt tgaaatatca atctgggcta
    95641 tgcagtatct gtttaatcct cctaatacaa cggaattacc ccccacccat aaagtgtttc
    95701 cctgcaaggt aagtgcatca attaaggagt tgctgcaata tatatttttg ctggtaacaa
    95761 aaccagatgt atcaatttca gctatccctt ttcttgtcaa accatcaata gcgctgaaat
    95821 tacctgatac atataatttt ccattctgag ccgctatttg attcaaataa tcggaattgc
    95881 taaagctaaa ggaaaaagta gtatcaatga ttccggtaag catatcaaac cggcacaggt
    95941 ttttaattgt agaattagtt aaataatggt taaaccatcc tgctgcataa atataatttc
    96001 ctgaagatat taattcacat ttatttacat taaaattgct gctaacccat atagtatcca
    96061 ataatagcgt atttaaatca aatcgtctga ccgcatcaac acttccaaaa atatcgcttg
    96121 cagtaaaata aacaaaattt cctgcattat ataaaccgta tcttccaatt ccaattaaat
    96181 ttaacgggat agagttaagg tttagtaagt ttccgctttg taaatcataa acagctatac
    96241 gatcaatcaa actccctgag atgcttgaaa aatctcctaa aacaaccaat tttgaattac
    96301 aaattatcat tttcgtaact tgtgcattgg gtacaggatt ccatggcaat atagcgcctg
    96361 tatttgcatc aattgcagca aggtatggac gggcactcgt tcctactgta tcaaattctc
    96421 ctccaatata aatcgtattg ttatatcgta caatacaatt tacacggtcg ttagctgtgg
    96481 gaatattggg ctgaacagtt tgtgagttca cttttacaga aagaataagt aatgcaaaaa
    96541 cggttacata atttttcatt gaatttgttt ttttattgtt attgtacaat atcgtcctaa
    96601 ataaaaaaga tagagtagta ataaagtaac tcaaagttat ctttgaggtt gaacaacaat
    96661 aatacttctc tgatgacaaa tctaagttta ttttccctac tttcaaagtc tccctcattt
    96721 gagaggactt tgaaataata aaaaaaacat tgaagggtaa atacgttttg aatccgtttc
    96781 atagtcaccg cgaaggggcg ggagactatg aaactataat gtatttgata gttctattgt
    96841 tttgcaataa aattaacctc tcataaaaat aattttacaa ccaccttctt actttctttt
    96901 tataattttc atattcctgt tcgaattcaa ttgccaaata tgattcttcg cgtttgataa
    96961 tgtattcctg aacaattaaa attagtatag gaaacagaat aatattccac cagttgccaa
    97021 tgaaaatagt cacgcctaaa taaacaattg ctaatcctac atacatcgga tttcttgtaa
    97081 tggcaaaaat gcctgttgtt tgtaatgaag atgccggttt aatcagaatt atggtgtttt
    97141 tggtaataaa aaactgcctt aaacttctga acaggaaaaa cagcgctgtg ataaaaagga
    97201 ttataccgag tgattttatt actaatgtat gcaatgccga atcaagaata ggaactttct
    97261 tctgtatata tacggcagct acaaaaatta aaacatagaa taatggcggt ggtatgtaaa
    97321 cacccggacc ttttgatgta tcaacctgtt tgttcattgt gctgttgtgt ttttcaatgt
    97381 taaatcatta tatcattctt gttttttaat attaacataa ttgattttta gttgtttcat
    97441 taaatcttcc agatagtcaa tctgcacttc ctgtatttct aataattttt tgttttgatg
    97501 tactaataag tgatctattt tttcacttag cagttttatt tcaagttctg ctttaagatt
    97561 aattttatag tcatgctctc ctctttgtct gtctttttgt tcctgcctgt tttggctcat
    97621 cattattata ggtgcttgta tggctgccag acaagaaagt attaagttta ataggataaa
    97681 tgggtaaggg tcaaatggct tacttttaag taaccaaatg tttatgatta tccacaaaag
    97741 cagaaaaaag aaaaacgaaa gaataaatgt ccagcttcca ccaaaagaag caattttatc
    97801 agcaagtttt tgacccattg tgagtttggc ttcaatttca tcctgaatat tttcagaaag
    97861 aatagaattg tcgcgaatgg cttgcattac atctaaatca atcgtagcta tttcgccttt
    97921 ttcctgttca acaagtttgg tcagataaag tcttctgtac ttattcaatt catcaattga
    97981 aataatacta tttgcatcga agtttgggaa gtcacttttt atcaaaagaa aaattccttc
    98041 tctgatttcc tgacctttta caatttcacc ttttgaaatt actttatttg atatataact
    98101 taacggcatt ttatattcat taaatgaaat tattcttttt tcttaatgta aaagtatttg
    98161 ttttaagaca tcaaactttc aatacaatta tattttaatg aatacatcaa tcaatgataa
    98221 agaatttttc agcacttcca tcactgaata tttttattaa aggggtgttt gtttttaatg
    98281 gtgattcacg tcccaataaa tctataattt ttataacctt tcttttaatg tcagttgttg
    98341 tctcagctat acctgttgtc aggcaggctg gggaataacc cgatgatgca tttaatgaaa
    98401 gtgcatagtt gtaaagttga tcacagatgt ttagtccgtt gccttcgcca ttgcttaaaa
    98461 cacaaagccc tattttgttt acagggtcga ggtatatttc agttgaaaca ccactttcgc
    98521 caccattatg tccccacacc caggcagaac caccattata atacaacact tctttatacc
    98581 agtttaaacc ctgacgtgtg ttgagtgatg gaaactgtac tgtccacatc tgattgatgg
    98641 atgcaggcga taaaattgaa tttgtaccaa atgttccacc atttaaatag gcaatcataa
    98701 aattggctaa gtcagtaaca ttgcttcgca gttgcccgtc aggataatcg gcaaaaccat
    98761 actgcggata ggcaacataa ttatttcctg aataatgata tgggatggca acatcatttg
    98821 gattaaaatt agaataatac catcttgttt tattcatgca aagtttgtta aagatgttta
    98881 tgttgcaata ttgatcaaac ggtattccac ttgcgcgttc aaccaaatat ccgtttaatg
    98941 ctgttgctat gtttgagtat tgaaacacac ttccgggagc agttgcaaga aaatttgctg
    99001 atgcattata atcgcttccg ctggtagaaa aatagcgctg catacaatca tctaaagtta
    99061 ttgtagggtc gggataacca tagtagccaa ccatagccgc ccagttatcc tgtatggatg
    99121 aagtatgtgt catcagctgg cggaatgaaa tagtgtcgtt gggataaccg ggtatttgta
    99181 acagccaagg taaatattga ttgacatctc catcaagatt tatcacattg ttttcgtaca
    99241 actgcatggc agcagtgcct gtaaaaactt ttgatacaga tgccattaaa aaaacagttg
    99301 tgtcgctaac ggggatagta tttgcaacat cggcaaatcc ataagagtta atccaaacaa
    99361 ttttatcatc cttaacaatc acggttgaaa cacccgggaa atgttgtgta ttcatttcgc
    99421 tctgaataaa agcatcaaca gaagtgcttg gattgggatt agactgacta aaagcaggta
    99481 aaacaacggt taagaaaaca atgatgaatc cttgtatctt aactattcgc ttagtcactg
    99541 tttttatatt ttaaaagctt acataagctt tgtgctttgc gaatatacga ttgttactgt
    99601 aaactattgt tactttattc ttgagtaaca attaatttct tacggtaact tgcttcacca
    99661 tttaatacgt ttacaaagta aactccggct gatatgtttc tcaggtttat tagtttttgt
    99721 gattcgttat tgataacttc acaaccaaca atttctccaa ctgcattata aatgttaacc
    99781 ttaccggtag gaattagttt atcaaacgaa atagtaaact gaccgtttgt agggttgggt
    99841 gaaagctgat atatgagatt gctattttct tttatactga cagtggtatc accacaaata
    99901 aaaacatcag cagcaattga tggtgttgaa accattatct gatttaatat aggaaaaggt
    99961 gaaccgattg ttggactaaa tatatttaca attcccggcc aatttgcact tgatggtgta
   100021 tttgtgaatt gcgttgttga acagcctgaa ttaccaatgc tatccgtctt tacaatataa
   100081 atatccttac caccaacacc aaaacttgtt gcataaccgg caataataaa tccgttatca
   100141 gttgtgtttt taacagcaaa ggagttgtct tcatctgctc ctccgtagtt tttgctccag
   100201 agaatatttc ctgttgaatc aatttttacg atagtcattt tgctggcgct gcttggtgca
   100261 acatcacata ttacggctgc aaaaccatta tcatgtgtta aacaacctga ttgagaaata
   100321 aagcgtcttc cggattcacc aatgtttttc gaccaaatga gattaccatc actgtcatat
   100381 tttataatct tagcatcaga ttgtccgata gcattattcc aggtgttgcc tgtagttatg
   100441 aaaccatcgt ttggtaaaat attcagtgag tagctgccat cattgttaat ttctccaata
   100501 gccttcgacc agattaatgt tccggacgag cttgttttca taatgtaatc atcaatcagg
   100561 cccgcaccaa agccacctac tgatccaatt aaaattaaat cgccattaga aagttcagca
   100621 acatcactgc cataaagata tcctccaaaa cttgactgcc aaagcatagt ccctgctaca
   100681 ctaaactttg caaggacagt attgcttcct gcagttccgc caactgatgc aagaagaagc
   100741 gtactatcga tagcctgctt taaatggatt ctccagtttt tattcggtac tgtataattt
   100801 atattaaaac tgttgaacca ctgaatatca cctgatgcat tcagcttaca gcaaaaaata
   100861 ttttcattgc cgtttccatt atcgtcataa ataccgcaag cataatattc accactaaat
   100921 gtttgtttaa tatccataat ataatcatcg ttggtgctgc caaatgattt tgtccattgc
   100981 atatttcctg ttgcatcaat tttaagaacc aaagcatcct tgcctccgtt tcccaaattg
   101041 gaagtatagc cgccaataat gtagccgcca tcacttgtta aattaacgga ataggcttct
   101101 tcgtcactga cactcccata aactttttga aatttaacct gagcttctgc cagagaaaca
   101161 aagcaaaaat tagcaattac gagtaatagt tttttcatat attttatttg gtattaattg
   101221 tttttttcgg aaagataaat taaaagtgtt acttaaaaat ctgcagcaaa atactgtgaa
   101281 agaaataata aaccaatagt taaaattatt tttaattagt tgatttgtat tgcactcaca
   101341 atgaatgaga ttgtgttata agaatgtagc accttaaaat ttaaatacct tcgcatcttt
   101401 caaaataaat tttatattac aagtatgggt ttcaaaactt tggataacat agctcaattg
   101461 aatgaaattg acacattatc aaaaaataag aagcaaatta tatttaagca tagcacccga
   101521 tgttcggtaa gtacttttgc taaaaaagtt cttttgtcag aattttctga tgatatggag
   101581 aaaaaatttg atgtgtatta tctcgattta ctcaattaca gagaggtgag taataaaatt
   101641 gcaaatcact atggtgtgta tcatgagtca ccgcaaattt taattatttc tgacgggaaa
   101701 tgtatttatg atgcttcaca tgatgatgtt tcattggcat cagcattggc atcggtcaat
   101761 taggactaat ttattgacag cgaaaaccca gcagcaacac atcgtcaacc tgatcgtttt
   101821 tgccgcgcca cttttttaaa tagtcagtaa agaaagttaa ttgctctttc atgggtaagt
   101881 tatgtttttc aagaagtagt tctttgagtt tgcctgacat gagcttttta tttttctcgc
   101941 ctccaaactg atcggcatag ccatcagtaa aaagatatac agtgcctgca tcgtcaaatg
   102001 aaatggtttg attgataaat tcctgttgtt gcaaaatgtt gttgccgcca ataactattt
   102061 tattccctga aagtgtttta agttcattgc ctgaaatgaa cacaagcgga cggtaagctc
   102121 ctgcaaatgt aatttgcttg gcactgcaat tgatatgaca tacggcaatg tccattcctt
   102181 cggcagcatg agtttctgcc tgattaagcg aacgcgaaac ttcaattcgc aactgattta
   102241 acaattctgc aggacgggta atatttttat ctctgacaag atgattgagc agcgatattc
   102301 ccaacatact cagcaatgca cctgcaacac cgtgaccggt acaatctgct actataacga
   102361 tgtgcccatc ttcaacaggt tcaatccaat aaaaatcacc agcaacaata tctttaggct
   102421 gatacagtac aaaactttct ttaaagaagg atgctaatgt ttcttcagag ggaaggatag
   102481 cgcgttgcaa gtaacttgca tagtgaatgg agtcaagaat ttctttgttt ttttcttcta
   102541 ccaatagttt ttgctgttgc agttcggcag ttcgttgttg aacctgagtt tcgagaagtt
   102601 cgttttgctg aagtattaat ttttcttttt ctaaaagtgc atccaatgct ttttgttgtg
   102661 cttcggcttt ctgaagcaat gcatcatcaa ggttgcgcga tgcttcaatt ccttttcgaa
   102721 gcatttccat ctctaaaaca gtttttttga tggttgcttc aaggtcggca aggtcaattg
   102781 gtttggtaat aaaatcaaaa gcaccacgat tcattgcagt acggatattg ctcaaatcgg
   102841 cataagcaga aacaacaaca gctttagtaa gaatattatt ctctctgagt ttttcaagta
   102901 aagtcaaccc atccatcaca ggcatgttga tgtctgtaag catcaggtca tagctgtcat
   102961 cattctttaa tttttctaat gcaattttac cattagacac atattcaaat tcaaattgat
   103021 tttctttaat ctgctttctg aatttttgtc tgactaaatc ttctatgtaa ggctcgtcat
   103081 ctactaataa tattttgctt ggcataagtt agggtatttt tattccaatt actaaaatgt
   103141 catcaacctg atcatgtttt ccttgccatt taattaatgt attcaataaa tattctttct
   103201 gttcattcat actcagatgt tgaatggata ataacagttc acgaaacttt tttgtcataa
   103261 actttttacc aaattctcca ccaaactgat caacaaagcc atcagaaaaa aggtagatag
   103321 catcaccttg ctgaagtgtg atgttatgtt gcataaaacc gtcagctttg ttgtactgct
   103381 gtccgccaat gggtattttg tttggtttat aaaactgaaa ttcgtttttt cgaatgagcc
   103441 aaagcggtcg gttggcaccg gaatattgta aggttttgtt tttgttgttt attgtacaaa
   103501 gcgcaatatc cataccttca ttcatctcgc tttcgcgttg tttgagtgat gttacaatgc
   103561 catcattaag ttgtgtaagt attttagatg gctctgtaat gcctttttca tttacaatct
   103621 gattgagcaa tgcagttcca atcatactca taaatgcacc tgcaactcca tgtcctgtgc
   103681 aatctgctgc cgctacaata attttttcat ctcgttgagc aaaactgtaa aaatcaccac
   103741 ttacaatatc ttttggaaga taaagtataa atgaatcatt aagtgattgg tttattttct
   103801 ttatgtcggg caatattgca gcctgaatgc gtttggcata gtggaggctt tctgttactt
   103861 cacgattttt tatctcaatt acattttttt gttctaatac ttcatgagta cgttccttta
   103921 cttccgattc aagcattgct ttctgatttt ccagcaaatg ctttttttct tgttcttgcc
   103981 tgatggttct ggtcgaaagt tcagttattt catctatctg ctttgttaat gtattgccca
   104041 tgcgtgcaaa gtcgcgtgca agatataatg ctacagaaaa tggcaaaccc attaacatac
   104101 ttacagaaat gaagttagaa gttttgactt caaatttcat cccccaaaaa gttattgtgt
   104161 caggtaaaat actgataatt attcctaaga ataccggtag gagtaaggtt agtgcaaaaa
   104221 tccaggcgcc atctcttttc tttcttacag atacaatgat tactctgaat atttcaacaa
   104281 atgacgttat aataataaca gccattagta tagcggctat tttgcgaaat ggcaataagt
   104341 aaagtacaat cattactgca taaagcccaa gcagtaccca taaatttctt cgaaaatgat
   104401 gataaaatat ggtgtgtaac atcgctacaa tgggcaccac tattaacggt gctaaaaact
   104461 gaagtgactt agagataaaa gcaattgatg aataatttga tgaagttaat aaatgatagg
   104521 tgtagaaagt aaaaataaag atgccaatgc aatagagact atagtacagg ttagtgattt
   104581 tttgcctata gtaaagaaac ataatgaggt gaactattgc taatgtgaga aaaactgctg
   104641 cgacaaaaag aattaacgat gattttttta tagtttgaat gctgtcgtca atttcactat
   104701 taaggtcttt gaatttgatt gtaaagtttt caccaatatt aaatccgctt attctggcag
   104761 aggaattgtg aaaatttgaa taacgtatgg caagaacgtg attatttaaa tttgaaaata
   104821 ctacagcaac gggttgtgga ttggtattga aaacagcttc ctcttcagaa atattagaag
   104881 caacagtgcc atattttttt aatagtctgc catctaaata tacctctgct gccgaaccat
   104941 ataaatgtaa aattaatgca agaggacggt tcaataaact actgtcaata tcaaaatgca
   105001 tacggtacca aactatcgta gtataatttt taatgtgatt acttctgata gtatctgaat
   105061 attcctccca tacagaatcg tcatatatgg gttctttcca taaggtatca tccccacgtt
   105121 taaatttcca gttattggta aactccaaca gattttcatt tctttcaaat acctgaatgg
   105181 tagaatctgc atttgcctga gcatcctcat catccgatag caaattgcca agattgacct
   105241 tgatatttac atttgagtcg agatcagatt ttttattgga attctgacca atgacattaa
   105301 aacaaattcc tgaaaagaaa atagaaagaa ataaccggtg caggttaaac atctgtaaat
   105361 tgaatttgca gtaaaggtaa ataaataact acttacagaa tgttgaccaa actgttacct
   105421 gactatttta acaaatcaat aatctttttt tctacaacgc tgctattcca gatgttttca
   105481 atgtcggcca ttttcataat ttcattcata atagcatctt gtctttttcc tcttgcctgt
   105541 gcctcatgat aaggaatgtg gctgaatgta accatggtat aaagtggaat ccatttgtca
   105601 ggatgttttg tgctgaacca tccttcaatt tttttgcgta ataagaattc aggcttgccc
   105661 actaaatcgc gcatctcaat aaaattgtca acggcaagtt cgccaatggc atcggcagca
   105721 ggttttctca ttcgttcgta ttcgggtatt actttctgcc agtcgtcagc atattgattg
   105781 agtaggtcgt tcaacacact gcaatcttca aaaccacagt tcattccctg accatagaat
   105841 ggcacaatgg catgtgctgc atcaccgata aggacaactt tgttatcaaa actccatggt
   105901 gcacacttaa cagtcaccaa tgaacctgtt gaattgtgaa aaaaatcttc ttcaagatcg
   105961 tgtagtaatg gaacagcatc aggaaaaact ttttgaaaga aagtttttac ttcttgtttg
   106021 ttgtgaagcg attcaaaaga aggattccct tttaatggaa agaatagtgt aacggtaaat
   106081 gttccatcaa gattaggtag tgctatgagc atatatccgc cacgaggcca gatgtgaagg
   106141 gcgttttttt cgagttgatg actgccatcg gctttagcaa caaacatcag ctctttatag
   106201 ccgtgttgca gataactctg cgaatagttg aactgttctg ttgatagctg tagctgaagt
   106261 cgtgaagcag caaaagcacc atcggcacca aaaatcaagt cggaggcaaa ggttgttttc
   106321 tgatgactct ctgagttttc gaaatgcatt tcgcctgatg caagtgttaa gcccgtacat
   106381 ctctgattaa aatgaatttc aacaccacct ttttctgcaa gatcaagtag ggtacagttt
   106441 aaaattcctc ttgaaactgc gtaaatgccc tgaccgtctt ttccgtaggg ttgtgtggtt
   106501 atttttccat caacactatg catggtgcgg tgtgtcatcg gtattccgat tttacggatt
   106561 gcatcatcaa taccaactcc ctgcaaacct cgccagccgc ggtcgctcaa tgcaaggtta
   106621 atacttcttc ctgcagggat ttttactttg cgcatatcag gtctgcgctc gtgaagtgta
   106681 actttatatc ctcgtttatt tagatataat gccagcaaag aaccaacaag accggcacct
   106741 atgattgaaa tttttttcat tgttgttatc tgtttagaag caaagcttcg tgcaggcatt
   106801 tgccaaattg ataaatgtcg ttaaatgaat tgtaaagcgg aacaggagca atgcgtaaca
   106861 cttcaggctc gcgccagtct gcaataatgc cttgctctgt caggtagcca tgaatttttt
   106921 tgccgttatg tttacatagc atactcacct gtgcagcacg ttcgttttca ttttcaggtg
   106981 ttatcatgat gatatttttt tctgaatttg tcttgctgat aatgtcacca attataaatc
   107041 gcagaaagtc attcatcagc ttgcgttttt ttatcagatt ttgtactccg gcttcggcaa
   107101 aaatttcaag tgaggcacgc aatgctgcca ttggaagcac aggggcatta ctcaactgcc
   107161 aagaatcggc acttttaaca ggaataaaat catgcggcat agtgaaacgt gtttgtggat
   107221 cgttgcccca ccaacctgca aatcgaatca gatcggtgtt gttacaatgt tgttgatgaa
   107281 cataagcacc tgcaacacca ccgggtccgg cattgaggta cttgtaacta caccatactg
   107341 caaagtcaac attccagtcg tgaagatgca attcaagatt acctatgcca tgagctaaat
   107401 cgaaacctac aattgctcct gcagcatgtc ctgcctgagt aattcctgcc atatcaaaaa
   107461 cctgccccgt atagtaattt gggttaccta aaaaaaccaa gaccagctca tctttatgct
   107521 ttgaaattgt ttcaagaata tcttcatgcc tgatggtatg ttcacctgtg cgcggatgca
   107581 actcaatgat ggcattatca ggattaaagc cgtgaaattt tacttgtgtt tgtaatgcat
   107641 actgatcaga tggaaatgcc gacccttcac agattatttt aaaacgtttt gttgttggtt
   107701 tgtaaaacga caccaacatg aaatgaatat tggcagtgag tgaattcata accacggttt
   107761 ctgacggtag tgcacctgct ataagtgcca tattatctct cagaaattcg tgatatggat
   107821 accatgggtt ttttgcctga aaatgtcctt caacaccgaa atctttccag tcgttcaact
   107881 cctgcaaaac atagtcgcgc gtttgtttag gttgaagtcc aagagagttg ccgcagaaat
   107941 agatgacatc tttattctga tgttgcggaa aaagaaattt ctgcctgaag gattttaaag
   108001 ggtcttgctc atcaagttga ttggcaaaat cggatgaatt gataaagtgc atgtaagtaa
   108061 tttgaataca aataaacaaa aaacattatc gaaaggatat aaaaacctgc tggtaaaaag
   108121 taaaattcaa ttttgtaaaa aagcgctgac taatgcctcg gcttctttgc atgattgatt
   108181 caattctcca ttgataataa cattatcgaa acggtcttgg tatgacaatt ctttaatgga
   108241 tttattaagg cgaatttcaa gagattctgc tgtttctgta ttgcgtgccg ttagtctttg
   108301 acgaaggtct tcaaccgatg gcggccgtat gaaaacagca agtaaatttt tgccaaaatg
   108361 ttttttaatc tttaatcctc cttcaacatc aacatcaaaa actactgaac agttaagcgc
   108421 ttctatacgt tcaacctctg attttaatgt gccatagtaa cgaccttcat acacttgctc
   108481 ccattcaaca aagtcattcc tgtcaatatg ttttctgaaa ttttctatag acataaaata
   108541 ataatctttt ccgtcagttt cattagcacg ctttgggcgt gtagttgctg aaatactgaa
   108601 ttgcataaaa tcaaacttcg ttaacagatg ttgtacgatg cttgttttgc cactaccact
   108661 cgggccacag aaaagaaaat gttttgtcat aaaatattag agagctgttc tttaattttt
   108721 tcagcttcgt ctttcatctc aacaactttg cgttgtatgt ctgcatcatt agctttgctg
   108781 ccaatagtat ttatttcacg accaatttcc tgcataataa agcctaactt tttgccatta
   108841 ttattttcat cattcaacat agataaaaaa tagcggcaat gctctgcaag tctcactttt
   108901 tcttctgtaa tgtctaattt ttcgagataa taaatcaact cttgttcaaa acggtttttg
   108961 tctgtttcta tatttccgcc aagagacttt aatgagctga ttagtttact gcgcactgca
   109021 tttattcgcc cttcctcaaa aggagttatt tgttcaagta attgcaaaat aagattcatg
   109081 cgttgcgaaa tatcttgttg cagattatgt ccttcgcgct gacggaaaag attaaatttt
   109141 tctattgcct gatttattat tgaaaaaata tatttccatt cattttcatc agcgtcatca
   109201 gaatcggtat ttactacctg aggcatttgc attacaattt gcaaaaggtt gtcatcgggc
   109261 aatgcaaatt cttttttaac tgcttttaaa tcggtcaaca gtgattttat tatttcacgg
   109321 ttgagtaaat tttcttgttg cgcaagatcg gactccatag taatcatcac atccatcttt
   109381 cctctttcag cttcttttaa taacaagctg cgcaggtcca tttctttatc tttgtaagga
   109441 tgtggcaggc gcatgttaag atcaaaaaat ttactgttga gcgattttat ctcaacagag
   109501 atagtcttac tcttatagtc accggaggct ttgccatagc cggtcatgga acgaatcata
   109561 cttcacaaaa ttttggctaa agtaatcaga atgataacag caacatcagc tattctcagt
   109621 attatttata cctgcaattt tttgcttact tactaaatag acacctgtaa aaattaaaac
   109681 agcagaggct aagtgcagcc aacccggact ggcttcgcca agagccattg aaattagtgt
   109741 agcaaaaaca ggctgaaaat aaatgtagat actcactaca gaagggctga gatgacgcaa
   109801 tgcaaacaca ttcatcagat aagcaacaaa agttacggct acaataacaa agagtatgca
   109861 aagccataag ttaaaagtca ggctgctcca tgctaccgct gttacttcat taaatccaaa
   109921 aggcaaaacc attaaggcac caaaaaagaa tgtccatttc atgacagtta taggatggta
   109981 gtgcaacatg agtggtttta ccaaaataat aaatatggca aatgaaacag agtttaagaa
   110041 aatataaaga tcaccttctg tgtttcctcc ctgaagattg aagttcttac ccaaaagtat
   110101 gagtaacata gcaccggcaa taccaagggt aattccaata tactttatcc nnnnnnnnnn
   110161 gttctttaag tattatggca gccatcagta aaaccaaaac agggtttgtt gtcattatta
   110221 atgcaccgtt tatgggtgat gtgagtgaaa gacctttaaa aaacaaaagt tgattgatgg
   110281 caacgccaaa gagtgcacag agaagaatag tgtttctgtc tttttttttg attttttctg
   110341 atggtattaa aaatgctgca agaaaaaaca aaactgtagc cggaattact ctgatgacaa
   110401 taaatccaaa aggtttaata aattcaggca tcacctgttt ggcaatgctg aagtttgccg
   110461 cataaagcag gttaacaaaa agaacagaaa ggtgagcgcg tgttgtggct ttcaagcctt
   110521 agtgattttt tgtttgattt ttatttttat gcattgcagc tttagctgca gcaactgttt
   110581 ttggtgcgtt tccaataaaa atttctttgt ctatgatgat aaccggtcgt ttcagaaaag
   110641 tatattcttc aagaatgtat tttttgtagt ctttctctga aagttttatc tgattgagtc
   110701 ccattgaacg gtatttcatg gctactctgc taaataacgc ctcatagctg cctgcaagtt
   110761 tttgcattgc atcaagctgc tgttccgtga tgggttcact ttttatatcc tgtaaaataa
   110821 aatcttttcc gggtttcagc tctgttaata ttctctgaca ggtggtgcag ttggcaagat
   110881 gatatatctt tttcattcaa ctttcttgtg taaaatagtt ttacaaagaa catgaaaaaa
   110941 catcagattt tggtattcct tatcttatgt tgtccatcac cggcaatttg ttttatcata
   111001 ttgcggaaaa taaaaaaatg aaaaggcaac atggaatacc agtaattcct gcccataata
   111061 ccaatgggtc tgaaagtagc agtttgtttc agaatacaac ggttgttgtt atgaatgata
   111121 gagaactcta accatgcttc gcccggaagt ttcatttctg catacaataa caatcggtga
   111181 ttttttctgt caaccaacag tacacgccag aagtcaagag tgtcacccgg ttcaatgtca
   111241 acatcacttc ttcgtccgcg actcatgcca acgccaccta ttagtttgtc aattattcca
   111301 cgcattctcc ataatgtatc ggcatagtac catccatttt gtccaccaat attaaaaaag
   111361 cggttggcta cttcatcagc tttgtctttg tctatttcaa cccacttgcg gtctttataa
   111421 catccgtttg acggtacttc aatatgttgg ttaacatcta agcgactcaa actgctcgat
   111481 gccgaatcgg tccaacttga aataaccatg ttttgttcta tccgtgcaaa cgccatttgt
   111541 attgcttcgc gataggtgta taactcctga ggaataacat cacgaacaga agtttcttta
   111601 cacaccacat catttttcat gctctgaact aaaagctttg caatggtgta gttggcaggt
   111661 gtagaaaaat aaatccatgt tgcagataag cccatgaaac caaaaggcag tgtaatgatt
   111721 aatcttttat atcctctttc tgctgcatat tcaagcaaca ttttacggta agtgagtaca
   111781 tcgggtcctc caatttcaaa aactctgtta aaagcttttt cattcatcag cgaaaggtgc
   111841 aggtagttca tcacatttct gatggctatg ggttggcatt ttgatttcag ccaacgtgga
   111901 acaatcataa caggaatttt ttctgttaat cctctgatga tttcaaaaga aatactaccg
   111961 gagcctacaa taatacctgc ttcaaatata gtgtaggcaa tgcctgaatt tataaaaaca
   112021 tctttcaccc ccttgcgtgc tgtcaggtgt ttggaaaggt tatcttcatt gctgatgcca
   112081 ctcagataaa tgatttgttt aacctgcgtg agcgtggcta ccaatacaaa acatcctgca
   112141 cagcgcagtt catattcttt aagcgttgtg gccgtatcgc ctaatgagtg aattaaataa
   112201 tacgcaacat caaattgctt gttgttaaat tgctgaatgg cgttttcagg ttccagaaaa
   112261 tcatattcaa taacatgtat tttatcaagt aaatcaggcg atggtttaaa gcgaagtgca
   112321 tctctcacca cacaggtaac atcatgtcct gcttctacca aaacaggcag cagcctcatg
   112381 ccaatatatc cgtttgcccc agtaagtaat atacgcatct tatacttgat gttttacgat
   112441 tcttcaatcc ttgcaaacat agcaggatat ttgatttcat gaaatgatat tgat
//
LOCUS       DAOOOI010000003        81940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_42_length_81940_cov_23.914148, whole genome shotgun sequence.
ACCESSION   DAOOOI010000003 DAOOOI010000000
VERSION     DAOOOI010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 81940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 81940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81940
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42_length_81940_cov_23.914148"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    440..450
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<451..688)
                     /locus_tag="PKX59_01075"
     CDS             complement(<451..688)
                     /locus_tag="PKX59_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89485.1"
                     /translation="MKRILLFIALASFSLTANSQANKTMNSWAELNNFHKVMSETYQS
                     SKDGNLKPIKAEYKQLIELAVALHQSKAPAGIDAA"
     gene            761..1306
                     /locus_tag="PKX59_01080"
     CDS             761..1306
                     /locus_tag="PKX59_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOZ89486.1"
                     /translation="MTRFIYSLFFKLFGWKIKGNIPYELKKYVMIVAPHTSNIDFFVG
                     LATRSILRLDTKYLAKKELFKPPLGWIFYALGGYPVDRSKPGKLVEKVAEIYNSHDQF
                     SICITPEGTRKYAAEWKTGFHKIAMAVKAPVVMVAFDYRTKTVVIAPPFTPSEDTQGD
                     IEKIKAWYRQFEGKIPEFGVK"
     gene            1416..1952
                     /locus_tag="PKX59_01085"
     CDS             1416..1952
                     /locus_tag="PKX59_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOZ89487.1"
                     /translation="MPTKIRLQRFGKKGQPFYHVVVADSRAPRDGKFIEKIGTYNPRT
                     IPATIDLNLDRALYWLGTGAQPTETTRAILSYKGVLYKNHLMGGVKKGALTQEQADAK
                     FSAWMENKNLKVLQHSQKVQSSKADDIKSRLVEESKKREVIAKAIAEKRAAANVAEAE
                     AAAEAPATDGGETPAENA"
     gene            1983..2501
                     /gene="rimM"
                     /locus_tag="PKX59_01090"
     CDS             1983..2501
                     /gene="rimM"
                     /locus_tag="PKX59_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOZ89488.1"
                     /translation="MGFDEVTFFGTIVKTHGIKGHLVLRCYDNFKCKKNNPVSLFIKE
                     GDFLKQVAVSEIQFTYPFCRIKFSDVADINEAETWLKKEVYIEKKVMLQNEIPYKYQL
                     IGCSVTDSISGPIGNIVDIAEYPQQQIAIVNANGKEVLIPLNKNFINAFNSTENNLAV
                     TLPDGLLDIYLQ"
     gene            2667..5099
                     /gene="ccsA"
                     /locus_tag="PKX59_01095"
     CDS             2667..5099
                     /gene="ccsA"
                     /locus_tag="PKX59_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOZ89489.1"
                     /translation="MEIKYIGEQLIWGYIGNGFIFAAFISAIYAAVLYLTSVRNEEKA
                     QWATPLARKFLYLHSISCFGIFFLLLYLIFNHRFEYYYVWQHSNMAMPMYYILACIWE
                     GQEGSFLLWMLWHSLIALFISRRNRHWESPVVGTIMMVQVFLGSMLLGVYIFGYKLGS
                     NPFTLLRDNDQFANLPFIKMPDYLSRIADGRGLNPLLQNYWMVIHPPTLFLGFASTVV
                     PFAFALGGLMRKNITEWIKPALPYAFFSIMTLGAGILMGAAWAYEALSFGGFWAWDPV
                     ENASLVPWLVMVGAGHVMLVFNKRGRALLSTYILCILSFLLVLYSTFLTRSGILGETS
                     VHAFTDLGMSGQLIFYMLFFVILAVVMLAINWKKIPKENSDDSITSREFWMFIGMLVL
                     CISAIQITFTTSIPVINKIFGSNLAPPVDVKEHFNSWQMPIAMIIALLMAVGQFFKYK
                     ESNPKEVFKKLLFSLIISLILSIVLILLLKFNSVHYMVLLFTAVFAIIANFDYFLRVL
                     KGKFSFSGASIAHIGFAFILLGTLISNANSRIISKNRLNIDLGKDFPNNENIMLAKGD
                     TLQMGKYFVTYSGREKKGHNIYFDVEYYKLNNKTGELKKAFSLKPIIQLNERMGNSPE
                     PATKWFPDKDIYTHVTYANLDEYTKNTSMDEYDTPTEHEMAVGDTLATSNSLVVLKKL
                     AKDNTGIEVESFDSFGVTAILEVTDVNKKKHVVTPQFLIKDRMAVSKNYFLDELGIKF
                     RFTSISPESGKIKLEIAEKKANKKDFIIMKAIIFPHINLLWIGCILMLTGTWIVSVKR
                     FKEYVRYTTDKG"
     gene            5071..5865
                     /locus_tag="PKX59_01100"
     CDS             5071..5865
                     /locus_tag="PKX59_01100"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOZ89490.1"
                     /translation="MSATPPTKDNRMTLTIIGSGNVAWLLAILFKTSGHKINQIYSRN
                     KKTGSALSKITGGKFIDDIQKIDSKSDIYIIAINDDSIAEVVSKLPALNNIVVHTSGT
                     VDLQILKKKFKKAGVVYPLQSITSESVLSDELTFCLEATNNTVLTSLKQLVTSVNCSF
                     VVMNSQKRAKLHLAAVLVNNFTNHLYAQAFSLLKKNRIEFNLLKPLISHTVKRINGNP
                     ASFQTGPAVRNDKKTIQQHLMLLKDNKQLADLYKQLTASIYKFHKK"
     gene            5862..6368
                     /locus_tag="PKX59_01105"
     CDS             5862..6368
                     /locus_tag="PKX59_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009388622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOZ89491.1"
                     /translation="MSNVNFKEKLKDIKCFIFDVDGVLTNGMLHVTNDGKLLRAMNIK
                     DGYALQLAVKKGYRVVIISGGKDEGVVIRLKNLGVSDIYVGIGNKLQQLLAVMQEHHL
                     TSAQLLYMGDDMPDAEAMKNCAIATCPYDAAPQIRDICHYISPVKGGDGCARDVIEQV
                     LTLNNDWE"
     gene            6368..7312
                     /locus_tag="PKX59_01110"
     CDS             6368..7312
                     /locus_tag="PKX59_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HOZ89492.1"
                     /translation="MGFLRLIRFPNLIILALAQCLIYYMIVVPLLKTSEFTPSLHTWQ
                     FLLVVLSTVFIAAGGYTINDFFDKDIDAANGIGKVHDFSGWTLKTFYFVFSIMGIAVG
                     LYLTYALKLRQFALTYLLTAALLYFYSASYKRLPLVGNFVVAFLTAVAVFLPAFADYE
                     LQYAFRDIKLPVINNKMYNLRLIIAITAAYAFFAFLISLVREVIKDMEDIDGDREFGC
                     NTLPIVAGINNAKLISIGLLLLIFGLILFLQIKQSWWESLPVFGYTILFVQVPLLILA
                     VKLYFSAEKKHFKVASMIAKIVMVGGILSLPVFKYFST"
     gene            7336..8640
                     /locus_tag="PKX59_01115"
     CDS             7336..8640
                     /locus_tag="PKX59_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOZ89493.1"
                     /translation="MLLVYTHKFSPRLKYIFDLVIGKLMGISFEITHNVEEFKSYAGP
                     KFNYSEHQIENEIFIYSERLLFEKGIEDQQFGFTEWDDIKAFYATHPKYSVPFDLFSA
                     SFYLVSRYEEYLPHLRDSHDRYNETESIAYTRGFLQKPVVNIWAQKFKSIILERYPSL
                     KSVSPKYKYISTIDIDNAYAYLEKGLMRTIGAYGRSLFNFDVPQIVERTKVLTRLMHD
                     PYHTYELMHDLHKRYKIKVIYFFLLGEYGENDKNVSVDNRNFQSLIQSLADYADAGIH
                     PSYGSNIKQGRLQKEVQLLTKILKREVTKSRQHFLKIRLPDTYRQLISVDIKEDYTMG
                     YAGNIGFRAGICSSFYFYDLDQEVQTPLLIHPFAVMDATFRYYLKVNPEQVIDLIKPL
                     VEEVKSVNGTFISLWHNESLSENHIWKGYRNIYEELLKIAAV"
     gene            8642..9553
                     /locus_tag="PKX59_01120"
     CDS             8642..9553
                     /locus_tag="PKX59_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89494.1"
                     /translation="MEVKFLEHIEIDKAKWDQSINQALNKLPYAFSWYLDAVSPNWHA
                     LVSDDYNFVFPLTWRKKLCFNYLCQPLFTQQLGIFSSQPIDFAISNAFLNAIPSKFKL
                     IEININSFNPAAGNKFIAAKRLNFELELNRPYEEIRKFYSDNQKRNINKAKRNELSLN
                     QPDISEVISLFRHDRGMKIERMKDESYELLRILYSTLKQKGLCYLRGVSDGNQTICGG
                     LFIQTDYRIIFLFSGNSVAGKSSGAMSLLIDSVIQENANKNLVLDFEGSNDFGLARYY
                     SSFGSIEHVYLHLKKNTLPFPVRLFKR"
     gene            9556..10203
                     /gene="upp"
                     /locus_tag="PKX59_01125"
     CDS             9556..10203
                     /gene="upp"
                     /locus_tag="PKX59_01125"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOZ89495.1"
                     /translation="MVNILSTGNSLLHHFVAELRDVEIQNDRMRFRRNLERIGEIAAY
                     EISKTMIYENTDVTTPLGIATVPLMQSQPVLGTILRAGLPLHQGLLNYFDQADNAFIS
                     AYRRHHKDGSFDIHLQYLSSPPIAGRVLILSDPMLATGQSLVETYKAMLESGQPAHTH
                     VVAAIASRQGIEYVKANLPDDLTIWVAAVDEELTAQSYIVPGLGDAGDLAFGEKK"
     gene            10255..10836
                     /locus_tag="PKX59_01130"
     CDS             10255..10836
                     /locus_tag="PKX59_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89496.1"
                     /translation="MGEFVKILMVILISSVKFVVGPAFAYYDEKYDFTIFEAIVYPII
                     GGMLGVFVFSFFSDQVANVWHWIKNRIKKTVSRKQPFTEPTIDSDGKVVVNYTYVDAK
                     EKPKNKIFTKRNRRIVTIWKKYGLFGIALLTPVIISIPIGTIIASRLVPDKKKVFLYM
                     LISISVWSIIMVSSFELYHAYSIKALQQKVLEP"
     gene            10833..11537
                     /locus_tag="PKX59_01135"
     CDS             10833..11537
                     /locus_tag="PKX59_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HOZ89497.1"
                     /translation="MKKLKHYSHLIFDLDHTLWDTNVNAAQSLSEMYHQYSLEQYGII
                     SEQDFIQKYVDINNRMWTEYSLGRIKKSYLRAGRFELTLKYFGIVNPELVEKLSDFFV
                     QNTPGKKELIPFAKDLLNHVHGKYQLAIITNGFSEAQHTKLRSSSIDHFFSKIIISEE
                     IGYHKPDPRIFLQTAELLQTPIKDCLMIGDNIETDIAGAIASGMDCAYLNPAGIKHNY
                     EVTFETHCLSELIKWL"
     gene            11542..12462
                     /gene="rsgA"
                     /locus_tag="PKX59_01140"
     CDS             11542..12462
                     /gene="rsgA"
                     /locus_tag="PKX59_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOZ89498.1"
                     /translation="MQTFEGIVVKTTGNIITVQTGNERILCRIKGNFRIKDINSTNPV
                     AVGDYVKYYFEGDDALITDLLDRKNYIIRKSKKLSKQVHILAANIDHAFLVATPVLPR
                     TSTGFIDRFLATAEAYSIKASIIFTKADIYNRDILNYVDELKNLYSGIGYSVYVVSSQ
                     IPDSLIPLRTQLKDKTNLFTGHSGVGKSSLINVLIPGLNLKVTPISEQHLTGKHSTTF
                     AEMHQLPEGGFIIDTPGIREFGTYDFNKNDVTHYFPEFFQLLPQCKFNNCIHVNESGC
                     EVLAALERGEVSPSRYHNYLSILNNEDIFR"
     gene            12466..12924
                     /gene="dtd"
                     /locus_tag="PKX59_01145"
     CDS             12466..12924
                     /gene="dtd"
                     /locus_tag="PKX59_01145"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014938315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOZ89499.1"
                     /translation="MKVVIQRVITASVEIEGAIYSSIHNGLLVLLGISVDDEKEDVDW
                     LCKKIVGLRIFSDENGLMNLSVKEINGQILLVSQFTLYGDANKGNRPSFIHAARPEKA
                     IPLYEYFIQQLNMNDIVVCTGKFGADMKVSLTNDGPVTIIIDSKKQTNDH"
     gene            12914..13240
                     /locus_tag="PKX59_01150"
     CDS             12914..13240
                     /locus_tag="PKX59_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOZ89500.1"
                     /translation="MTIKDSQQIVDDWIKKHGVRYFNELTNLALLTEEVGELARIIAR
                     KYGEQSAKNNEDSLEMADEMADILFVLICLANQTGVDLEQAMKKNLEKKTHRDAGRHL
                     KNKKLQ"
     gene            complement(13241..13978)
                     /locus_tag="PKX59_01155"
     CDS             complement(13241..13978)
                     /locus_tag="PKX59_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89501.1"
                     /translation="MKTSKLNLNFFIVIILIAFFSTGCSPIYYVPSQVNMPSLTKGGD
                     AKFSASFALPQFNGVAQSFDGQLSYSPVKHLGVTVDGLAVVGTGKDKDYSGSGKSFGF
                     ALGYYNMIDENLGVEFYAGYGGGDVKLNRDSLKFFSADVRRFYIQPGIYRSTKRFNFG
                     IAFRFSNVQYSNLVFYQIPEIGDKIVEDNYFFYEPSFYCSLGGNLVKVKFQLTPSFNI
                     DNKLDFNRQKLAIGLGVDFLINRKNRD"
     gene            14185..15057
                     /locus_tag="PKX59_01160"
     CDS             14185..15057
                     /locus_tag="PKX59_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOZ89502.1"
                     /translation="MSHQKAIQTTWISIAGNILLAVVKWITGYFGNSYALIADAIEST
                     TDIFSSLLVLLGLKYASRPADKNHPYGHGRVEPLVTFLVVGILVASATLIAVESIRNI
                     MTPHQTPESYTLIVLAVIIIFKELFFRFVKKRNSQINSTALKADAWHHRSDAITSLAA
                     FIGIAIAIFMGKGFEAADDWAALVAAGFIFYNSYLIFRPALGEIMDEHLYEDVVEDIR
                     KVAGKVEGVIDTEKCYVRKTGINFLVDLHITVSSHISVKAGHQIAHHLKDVLIKHNPL
                     IADVLIHVEPDEHV"
     gene            complement(15083..15949)
                     /locus_tag="PKX59_01165"
     CDS             complement(15083..15949)
                     /locus_tag="PKX59_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOZ89503.1"
                     /translation="MRQLKITKSITNRESASFEKYLQEIGKEELITAQMEVELARKIR
                     AGDQRALEKLVRANLRFVVSVAKQYQSQGLSLPDLINEGNLGLIKAAKRFDETRGFKF
                     ISYAVWWIRQSIMQALAEQARIVRLPLNQIGAANKVKKAFSSLEQKFEREPTAEELAN
                     ELEIDTDRIAYTMKMPGRHVSMDAPFVPGEDNTLLDVLTNPDAPYADSTLMAESLHTE
                     INRSLETLPEREKDVIKLFYGIGVEHGLSLEEIGEKFDLTRERVRQLKEKAIKTLRQN
                     SKSKLLKAYLGQ"
     gene            16443..16901
                     /locus_tag="PKX59_01170"
     CDS             16443..16901
                     /locus_tag="PKX59_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89504.1"
                     /translation="MIKTTNIFTALVCLLLVTSLSVLGQKGKVNQEKKITGPGKCLIK
                     SDSLLTNELTKEDLIKWVENPPFIVNCEDSKTYKLKFYEFTILKLKPFENKSFGVGDD
                     RMIPILGRKAIDDLKEGDTVILKGVDLIEDGTDKTTKAPTISIKIIEKKP"
     gene            complement(16898..17815)
                     /locus_tag="PKX59_01175"
     CDS             complement(16898..17815)
                     /locus_tag="PKX59_01175"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HOZ89505.1"
                     /translation="MIFLIGIFSNSFCQDSVNTQRKIPFFEPSPVYNKQRVQIAGYTT
                     LGLYAGTMAGLYQLWYSGYDMNHFHFFNDNGEWLLMDKTGHTFSSYIAGKQGYNVCCW
                     AGMDNKKATLIGGNLGWIFLVSVEALDGFSSAWGFSWGDIAANTLGSSVFIGQQLLWQ
                     EQRIEFKFSFHQSKYAHYRPDLLGENYTQQLFKDYNGQTLWLSANINSFIKKTNLVPA
                     WLNIAAGIGAEGMTGAFDNATEYKNNPIPNFKRYRQIYIAPDIDLTKIKTKSKALKLF
                     FEVTRFIKFPLPALEINSKGKWLFHPLYF"
     gene            complement(17971..18342)
                     /locus_tag="PKX59_01180"
     CDS             complement(17971..18342)
                     /locus_tag="PKX59_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOZ89506.1"
                     /translation="MQHDDLHIEKLYYSIGEVADMFKVNTSLLRYWEKEFTMLHPKKN
                     LKGERHYTKKDIESFRIIYHLVKEKGFTLSGAKQYLKTNGKAIPVAEVSENTEVKEDN
                     SHVKFALNRLKAFLIEMKGNL"
     gene            complement(18373..19344)
                     /locus_tag="PKX59_01185"
     CDS             complement(18373..19344)
                     /locus_tag="PKX59_01185"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOZ89507.1"
                     /translation="MAKAKYYFNTHSLKYEKVVVSFKKRFLRVLGWLATAMVFSAIII
                     ALAYQFLDSPKEKQLKREIAVMDLQYEILQQRMDLMSKVLKDIEYNDDNIYRVIFEAD
                     PIPESVREAGYGGVDKYRSLKGYDNSSLMIETTTQLDKLMRQMYVQSKSFDEVFELAK
                     NKKELLASIPGIQPVSNKELTRIASGFGYRIHPIYKTSILHTGIDFSAPIGTEIYSTG
                     NGVVKKVEKEGRGYGNNVTINHGFGYETLYAHMSKIIVKPGQKVIRGQIIGYVGNTGS
                     STGPHCHYEVIKNGSKINPINFFFNDLTAAQYEQVLEISSKYNQSFD"
     gene            19478..22096
                     /gene="alaS"
                     /locus_tag="PKX59_01190"
     CDS             19478..22096
                     /gene="alaS"
                     /locus_tag="PKX59_01190"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOZ89508.1"
                     /translation="MTSSEIRKTFLDFFQSKQHTIVPSAPIVVKGDPTLMFTNAGMNQ
                     FKDWFLGNQPAKYRRVADTQKCLRVSGKHNDLEEVGVDTYHHTMFEMLGNWSFGDPND
                     PNAGYFKKEAIAWAWELLTEVYKLPKDRLYVTVFEGDKKENLHFDEESNTCWKQHIDH
                     SRILMGNKKDNFWEMGDTGPCGPCSEIHVDLRSKEERKQTDGKILVNTGHPLVIEIWN
                     NVFMEFNRKADGTLEKLPARHVDTGMGFERLCMAIQQKKSNYDTDIFQPTINYIEAMS
                     GIKYGSSEQTDIAMRVLSDHIRAIAFTIADGQLPSNNKAGYVIRRILRRAVRYSFTYL
                     NFKTPFIHRLTPLLADQFKEVFPELFEQKDFVQKVIKEEEQSFLRTLETGIRRFDLYN
                     KSVIDGEFAFELYDTYGFPIDLTQLIARERNMQVDMNGFNQCLEQQKERSRAASAVDA
                     EDWVILENSESKFIGYDNTTAEVNIIKYRKVKSKNKEQYQLVFNQTPFYAESGGQVGD
                     TGYIQNEKEKIFITDTQKENNQIIHLSDKLPVDLKADFIATVNNSRRRKITANHSATH
                     LMHAALKQILGKHVVQKGSLVNDEHTRFDFAHFAKVSDEDIAKIEQLVNEKIRENIAL
                     DERRNVPIEEAKSMGATALFGEKYGDYVRVIIFDPAYSMELCGGTHVNATGEIGLFKI
                     LSESAVAAGVRRIEAITSIEAEKYIINQLTQLNTIKTELKNPKDIVIRVKELLSENEI
                     LQRQIDGFVHEKAQLIKADLLKNVKLQNGINVIAQKIDLTNADAIKNISFELRNQIPD
                     SFILLASENDGKAMLSLIISDSLVQSGNLNATTVIRNISKHIQGGGGGQPFYATAGGK
                     NPAGIDAAIEEALKMI"
     gene            complement(22151..23002)
                     /gene="gldN"
                     /locus_tag="PKX59_01195"
     CDS             complement(22151..23002)
                     /gene="gldN"
                     /locus_tag="PKX59_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HOZ89509.1"
                     /translation="MKKVLLFCFLFVMTFMVKQTNAQTVLDGVYVKEHNPTRKVMEYS
                     FLREADVLWAKRVWEVIDLKQKINLPLRFPREGDTKDRKSLIEVLMNAIDEGSLTAYS
                     TTDDEFTTPMTLEEFKKIGGAGMDTIQITDPEPPYLTRDSAIQRTFSRDKVIAYRIKE
                     EWFFDKQRSVMECRIIGIAPLMLAEDEYGNIREGNIQKPLFWIYYPEARKLLANAEAF
                     NRENDTERRTFDDVFHKRMFNSYIIKESNVYNRRIEDYKTGLDALLEAERIKSEIVNL
                     EHDMWEY"
     gene            complement(23033..24637)
                     /gene="gldM"
                     /locus_tag="PKX59_01200"
     CDS             complement(23033..24637)
                     /gene="gldM"
                     /locus_tag="PKX59_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HOZ89510.1"
                     /translation="MASGKLTPRQKMINMMYLVLTALLALNVSKEIINAFITIDEGIT
                     ASNNNVIKKNNQVMDAFATAAQADAVKAKPFQEKATQVHKASDDLYKYLEDLKNEIVR
                     KADKLEANAKIPAPREIQKNDDYDTPTLIMCGDKQDGKGFKATELKKKLEDYKVKLIG
                     FVADKDKKDFQSRFDELINLKDPDPASKLAKEEKKKTWEMATFYHTPVVAALAMLSKI
                     QSDVKTAESQVNNYLLAQINAKDLKFTDVEPKVVAPTSYVLTGQDYKADVFLAAFNKS
                     SDAEIVVGGRSLPVENGLGKYVDRPSSEGVKKWGGVIKVKDPEDPTKVKEYPFEAEYI
                     AAKPAAVVSPTKMNVFYIGPENPVSISVPGVPNNKVVASISGGGGSLTKNPKGTGADY
                     IVKVTTQGEATISIIADMDGKKINMGAQKFRVKRVPDPVAKIAGKREGPMQKNALIAQ
                     SAVIAELENFDFEIYYKVTKFKMSLYPKGKDPIELESNNNLITANMKAALSKARAGDK
                     VYFEYIKAVGPDGGTRSIPSVNFTIQ"
     gene            complement(24694..25458)
                     /gene="gldL"
                     /locus_tag="PKX59_01205"
     CDS             complement(24694..25458)
                     /gene="gldL"
                     /locus_tag="PKX59_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008203296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HOZ89511.1"
                     /translation="MAKLYGIGAAIVIAGALFKIQHWEGAGIMLTVGLLTEAIIFFFS
                     AFEPLHEETDWSLVYPELAGMHDPSQGRDKKKKIDPVSQALDKMLSDAKIGPDLIASL
                     GTGLKSLGDNTAKMADLSDASVATNDYVKNVNAATKSVTELSGSYQKAAQAMSSLSTA
                     SDDVKMYNEQVQMAGKNLGALNAVYELQLQDSNNHLKQTSKFYDGINQLMSNLNESLD
                     DTKKYKDEVSKLAKNLSSLNTVYGNMLSAMNLNPNK"
     gene            complement(25578..26840)
                     /locus_tag="PKX59_01210"
     CDS             complement(25578..26840)
                     /locus_tag="PKX59_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOZ89512.1"
                     /translation="MRKLFIPIIAVILLSSCGGGNKGQLVGALNRERWYQADPYGMVF
                     IPSGAYNMGPSDQDVPYAMVAQSKSVSVHAFYMDNTEITNNEYRQFVEWVRDSLAHRL
                     IGGDHLIEEGEYGERINWRKKIKWEDEQNVETLAELFLPESERFYRRKEIDSRKLNFE
                     YYWIDLKEAAQKSNREQGMTDRSVFIKKDIINVYPDTLAWIHDYTYSYNDPVTNMYFW
                     HPAYDDYPVVGVTWKQARAFCIWRTQLLNSYLQENGESFVQDFRLPTESEWEYAARGG
                     HDLAPYPWGGPYIRNSRGCFLGNFKPMRGNYMDDGGFYTVKTTSYWPNDYGLYCMSGN
                     VSEWTSNAYEEAAYNFSHDLNPDYSYDAKDSDPVALKRKVIRGGSWKDVGYFLQTGTR
                     SYEYQDTAKCYVGFRCVMSFLGRDKADF"
     gene            complement(26990..27892)
                     /locus_tag="PKX59_01215"
     CDS             complement(26990..27892)
                     /locus_tag="PKX59_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOZ89513.1"
                     /translation="MKRLFTVVAVATASVSFGQQDPQFSQNMFNRLYPNPASAGTNEA
                     ICGTLIYRDQWDKFGGGPKTAVFGVESPVEVLHGGLGLSVLTDKPLNQKNLNIKLAYS
                     YHLDLGSGKLGIGLDAGLLQRGYGNDFNAIDPDDPLVKSGTGSKFDLGAGLYYKSDKF
                     YVGASATHLTGGKIEYTDDAKVEVVPHFYFMGGVDFDLTPSLKLKPSLFIKSDAKETQ
                     ADINANLHIKDKVWIGASYRLQDAIVLMAGINIIENLRLGYSYDITTSKLKGYHSGTH
                     EIMLGYCYVIKPKVIPVLRNVRFL"
     gene            complement(28021..29193)
                     /locus_tag="PKX59_01220"
     CDS             complement(28021..29193)
                     /locus_tag="PKX59_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HOZ89514.1"
                     /translation="MQISEFFSPISESIEQEMSNAPIDCMWQHVEKNLGSSGFPFMNN
                     IKIAIVGVEETRGAKKDYEAKNGVDNVRRSFYRLKKHGVGVPVADLGNVRLGVTVEDT
                     YAALTEIVISLLQLKIIPVIIGASQDLTVAHYRAYKKIDQIINMVGVDARFDLGMPSD
                     PLDSVSWLGNIVMDQPNHLYNYSNIAHQTYFVGDYAVNMMDNLYFDTYRLGEVNQDSI
                     EMEPTIRNADVMTFDLSSIRFSDASATSTPSPNGLTGDMACQIMYYAGMSDKLEGIGL
                     YEYAASIDTNQMTAALISQMVWYFMEGVSNRKNDFPDSTSSQFTVYNLTLRENNEEMH
                     FLKSNVTGRWWMEIPLSKIKKKLDRHQLVPCSYDDYQKACNNEIPERWWQALKKLS"
     gene            complement(29196..31802)
                     /gene="topA"
                     /locus_tag="PKX59_01225"
     CDS             complement(29196..31802)
                     /gene="topA"
                     /locus_tag="PKX59_01225"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOZ89515.1"
                     /translation="MAENLVIVESPAKAKTIEGFLGKDFVVKSSYGHVRDLAKTNNGI
                     DIKNNFEPIYEVSEDKTKVIDELTKLSRKAKTVWLATDEDREGEAISWHLSEVLNLKD
                     SATKRIVFHEITKDAILEAIQNPRKIDIHLVDAQQARRILDRLVGFELSPILWKKVKP
                     SLSAGRVQSVAVRLIVEREREIQDFGVTSFYKVVANFLVEDKSGNTTLLKAELDKKFG
                     TEKEAEAFLEKNKNATFNIASLEKKPSARKPAAPFTTSTLQQEASRKLGYAVATTMSI
                     AQKLYESGMITYMRTDSVNLSEQALTNAEKEISTSYGKNYHQKRKYQTKSASAQEAHE
                     AIRPTSFNRSVIKGTPGEQRLYDLIWKRAIASQMADAKLERTIVKIASDKSTEKFVAT
                     GEVIIFDGFLKVYTESSDDENEEDNDTLLPPLKEGQKLDYKSITATQRFSQPPARFTE
                     ASLVKKLEELGIGRPSTYAPTISTIQKRGYVIKEERQGKERKYAVLELSNSKINKVEK
                     TEITGAEKNKMYPTDTGIVVNDFLVEYFKDILDYNFTAEIEKEFDEIAEGKLKWNKMI
                     AGFYKPFHKNVENTIETSDRATGERALGKDPKTKRKVIVRLGRFGPMAEIVAEKESDT
                     PMYASLQKNQRLETVTLEEVLDLFKLPRHFGQYKNKELIVNNGRYGPYIKFGEEYISL
                     PKEEDPFTIEIESIKAILDGPRLPRTLGQHKGLDIIINKGRFGPYIKYGTEFISLPKG
                     EDPLEIDLETVVDILSRPRLPRNAGQYKGKDVVIAKGRFGPYIKYDDLFVALKKGDDP
                     FEVNIDYLGELIDAKLSGVPLKKEKATKTEKASAAKKSKSKSSAKKSTTTKKKAAVST
                     KTKSKAKSKSGR"
     gene            32001..33473
                     /gene="miaB"
                     /locus_tag="PKX59_01230"
     CDS             32001..33473
                     /gene="miaB"
                     /locus_tag="PKX59_01230"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOZ89516.1"
                     /translation="MHNDIKVIDESRQGEPTMLQSSSENLSPKKLYLESYGCQMNFAD
                     SEIVASILKNEGFETTKNVNEADAIFVNTCAIRDNAEQRVRTRLKDFKKIKKENPDLI
                     VGVLGCMAERIKSQFLEEEKLVDIVVGPDAYRDLPLLIEKVEDGQKAVNVILSKEETY
                     ADITPVRLGNNGVTAFVSIMRGCDNMCSFCVVPFTRGRERSRDPQSIIKEVEQLFNEG
                     YREVTLLGQNVDSYLWNGGGLKKDLDTETVHLALNHKLPSEQQRDFVTFSNLLEMVAE
                     VNPAMRVRFSTSNPRDMTDAVLYSMAKYENICKYIHLPVQSGNSRVLEKMNRGYSRED
                     YITRIESIKRILPDCGISTDIIAGFCSETEEEHQDTISLMEQTIYDFAFMFVYSERPG
                     TFAERKFTDNVLPEVKTRRLNEIVALQQKHSAIRTNQSIGKTFKVLIEGVSKKSDEML
                     MGRNSQNTVVVFPKNNHKPGEYVNVKVNKCTSTTLIGEVV"
     gene            33556..34776
                     /locus_tag="PKX59_01235"
     CDS             33556..34776
                     /locus_tag="PKX59_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOZ89517.1"
                     /translation="MTIQDIKARFGIIGFSSVLDHALNTARQVAPTDLTVLITGESGS
                     GKEVFPQIIHQLSSRKHGPYIAVNCGAMPEGTIDSELFGHEKGAFTGAHEMRKGYFEV
                     SNGGTIFLDEVAELPMFTQVRLLRVLESGEFLKVGSSKVQKTDVRVVAATNVNIPEAI
                     SRGRFREDLYYRLNTVPIHVPALRERTEDVHLLFRKFAGDFAEKYRMPALTLQPEALR
                     LLMEYRWPGNVRQLKNVTEQLSIIEQKKEISAEVLLKYLPQENFASVPTLFNNNKGGD
                     LNERELLYKVLFEMKKDISELKKIVFNIISNGEPVHLENSGIDTERFLNDVQGHPVLH
                     TNDTAITLTKPTHIDVKPHEHIEESLSIADREKDLIIRALEKYRGKRKGAAKELGISE
                     RTLYRKINEYGIKD"
     gene            34776..35318
                     /locus_tag="PKX59_01240"
     CDS             34776..35318
                     /locus_tag="PKX59_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOZ89518.1"
                     /translation="MSNIIMISGLLKRKLFSGLIVLIAFTVMMESCGVYSFTGASITP
                     GVKTVSIQYFPNRASVAQPSLSQVFTDKLKEKFVSQTSLIQVNQSGDLRFEGYISDYT
                     VQPTAIQSTDVAALNRLTITVHVKFENMKDEKQNFETSFSRYADFDSRKDLTSVESSL
                     MNDISSQLVDDIFNKSVVNW"
     gene            35318..36745
                     /locus_tag="PKX59_01245"
     CDS             35318..36745
                     /locus_tag="PKX59_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89519.1"
                     /translation="MTKERFFNFIQNPLSVHANDIEELEQIIEQFPYFQTARLIYTKS
                     LHNENSYLYNDELKRTAAFAADRSVLYKLIHSIKKDVTIDEPESLITEDIGSKSVINP
                     VQEVPDLNVFVFEEKTEIEEQAIVFEEKENSESADDELIIFEEKTLTIEEGEPIVFEE
                     KRPEITTQEDIVFTAVDEPINASAEDDEVGSSNKIAEIESEKKERVLTPAEILSQRLR
                     EIESQLEDKKEDETEVVSELKEETIIPETPQIAEPLNIQSEVLPESIQISSSIPEIKE
                     EVAEISEQAEAEKLIPAEPESSEKTIEVVAISPQIQPPVIEEVVAPDAIVQAEIKPLP
                     EADKKEKHSFTDWLHRFHPQEEKKTLSPDNLEEDNYNNKLINSQLTSYIETKNSNNEI
                     VNQFIQNEPRIDSSKTKFFSPGNMAKSSVTDVSDIVSETLAKIYLGQNNFSKAIQTYE
                     KLMLKYPEKSVYFAALIKEIKKSQI"
     gene            36758..37123
                     /gene="secG"
                     /locus_tag="PKX59_01250"
     CDS             36758..37123
                     /gene="secG"
                     /locus_tag="PKX59_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOZ89520.1"
                     /translation="MYTLITVLIILISVLLCLAVLVQNSKGGGLASGFSAPQQIMGVR
                     KTTDFLEKFTWGLGTALIVFCVVANFMTPGSNNSTEVRASSIQEQIDNATLPAAPQAP
                     LPSQQNAQPAQQPAAQPAN"
     gene            37306..37584
                     /gene="groES"
                     /locus_tag="PKX59_01255"
     CDS             37306..37584
                     /gene="groES"
                     /locus_tag="PKX59_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOZ89521.1"
                     /translation="MSKISLKPLADRVIVEVAAAEEKTASGIIIPDTAKEKPQKGKVV
                     AVGGGKKDEPMTVKVGDSVLYGKYAGTEISVEGKEYLIMRESDIFAIV"
     gene            37638..39269
                     /gene="groL"
                     /locus_tag="PKX59_01260"
     CDS             37638..39269
                     /gene="groL"
                     /locus_tag="PKX59_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611460.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOZ89522.1"
                     /translation="MAKEITFNLDARDALKKGVDALANAVKVTLGPKGRNVIIDKKFG
                     SPTITKDGVTVAKEIELTNSIENMGAQMLKEVASKTADIAGDGTTTATVLAQSIVNTG
                     LKNVTAGANPMDLKRGIDKAVEAVVASLKKMSKEVGDDNKKIEQVATISANNDSEIGK
                     LIAEAMAKVKREGVITVEEAKGTETTVEIVEGMQFDRGYISPYFVTNADKMQAVLESP
                     YILLYDKKVSNMKELLPILEKVVQTGKPLLIIAEDVDGEALATLVVNKIRGSLKIAAV
                     KSPGFGDRRKAMMEDIAILTGGTLIAEEQGFKLENADLSYLGTCEKITIDKDNTTIVG
                     GAGKKADITARVNQIKAQIETTTSDYDKEKLQERLAKLAGGVAVLYVGAATEVEMKEK
                     KDRVDDALHATRAAVEEGIVAGGGVAYIRAITALDKLKGDNDDETTGIAIIKRALEEP
                     LRQIVSNAGIEGSIVVQKVKEGKDDFGYNARSDKYENMHAAGVIDPTKVTRVALENAA
                     SVAGMLLTTECVLADIKEDKPAMPAMPGGMGGMDY"
     gene            complement(39548..41239)
                     /locus_tag="PKX59_01265"
     CDS             complement(39548..41239)
                     /locus_tag="PKX59_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOZ89523.1"
                     /translation="MKNLILTGLTIGLTCMMSYAQKYDEMKTTWEIETTHPFTETGLS
                     EKKEIVHGSNEKMFTMVSAVDGKVKWSKKFKEIDEQIGKVDLQIPMYESNAVFLFDKK
                     AGKDQMVVVDMETGTKLWSTNKYQGIDDPDEVIYIAELNAYAIVTKTNLTMVKARTGE
                     ELWSTVKFSTPVGKYFYDEKENAIVIISMPRNAWQAMFKGFRNQILKINAKNGDILWE
                     QTFVGIVERKVVTRESVAKIFLRDDKLFLQLNGLQVYEYKTGSPIWAAAYDVTFAREL
                     VRPKVYGQVVKYGVYDAIAQPLYDGEYVYILDVKSRKSQYLKKYEINSGKLVWSSPEI
                     KDARVIPGLYKIDDRIIVQIGGAVEVQAISKETYGNGYVQWTQYYRTVKYQNVKPYEV
                     QAFSTSDGTKSWDSERFRKGITNIFPSGNNIVVSSGKALYSLDYKTGKEAYEIPLGDD
                     DIGLAEKIVNPTELGDNISKDNVIVLGEKGVSSHNIATGAKVWAVKTKKGEFSGIFGE
                     NIFFDTEKGDYFSIDANSGKAKFFDSRKNSKSIVSLDGKSIYVFEGKKILKVSTE"
     gene            complement(41281..42792)
                     /locus_tag="PKX59_01270"
     CDS             complement(41281..42792)
                     /locus_tag="PKX59_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89524.1"
                     /translation="MKPILLAILSLAFVTTVYAQPAGTRTITIDDCNYPILGIETNHA
                     SDKIVLTASQGRIFFINVSDVEANTEVNPVWKNLNLSGLNNGGKPQFSENDQYILLQE
                     SSALKTAITSRKVKDIKYYILNAADGKVVSEGNNVNSVQFLSNSTQILIASNDGIMVK
                     DFLSNKVIAQLEVDNCEMAAISHDNKLIALSFDPDKADFKEVESIGKNKSELKTARKN
                     KRLISFYSYPDLKKLYNSKEEIDIVYSIKFSRDDKQTYLLSHNVVAERIGTAGLNGIS
                     MAAADNRRQTNIQVLDASNGNLVKEIYHATAEPDADYKFSTYSNYFGYSDNDGFIGWK
                     RKLVLIDNNDHKNKVGQYRYQGRRGSSNIFPIYFSFNKTKPVAYLSNGTKLIEWNYET
                     LKEYTDYIEGQDNDSLQSKAISQLNADLENPESDLSKNIVKQNIKGIYLFDLTVQKKG
                     QIVTIFAESDDKTDIPHQNALKDLLLKYKFKDIVVSPNQRLKFRYTFSIENNI"
     gene            complement(43229..43564)
                     /locus_tag="PKX59_01275"
     CDS             complement(43229..43564)
                     /locus_tag="PKX59_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89525.1"
                     /translation="MEQLSNQNFQQRNLIEQTVVQLNKDLIPCGFTVAWSGDTFSAYQ
                     DIIMQLEVIIRQLAKSGSSTLNAWLYRVDIPEKTLKKVLNKQDNQQALAQAILERTFI
                     KIMFRNSYR"
     gene            complement(43586..44011)
                     /gene="aroQ"
                     /locus_tag="PKX59_01280"
     CDS             complement(43586..44011)
                     /gene="aroQ"
                     /locus_tag="PKX59_01280"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOZ89526.1"
                     /translation="MAKRIVIINGPNLNLLGKREPEIYGHQTFDDFAITLKKEFPEPN
                     IQIFQSNVEGEIINLLHNEGFKADGIIINAGGYTHTSVAIADAIAAIPSPVVEVHISN
                     IHSREEYRHKSLLAANCKGVITGFGLNSYRLALLALLNS"
     gene            complement(44018..44599)
                     /locus_tag="PKX59_01285"
     CDS             complement(44018..44599)
                     /locus_tag="PKX59_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOZ89527.1"
                     /translation="MEKFYTIQGEGYHTGKPAFFIRLAGCDVGCVWCDVKESWEASTS
                     NLISVDNLLKAVAESKTPNVVITGGEPAAYNLTKLTDALSKKGYKVWLETSGAYPIQG
                     TFDWICVSPKKFKAPLESVLQKANELKVVIFNESDFKWAEENARKASDVAKLFLQPEY
                     SRFDRVIPIMIDYIKRNPQWSISLQTHKYLNIP"
     gene            complement(44681..45628)
                     /locus_tag="PKX59_01290"
     CDS             complement(44681..45628)
                     /locus_tag="PKX59_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HOZ89528.1"
                     /translation="MASKIKFIEVKSEIGAGTRGASLGVDAIKIAALDYGSALFNRIE
                     SVEIKTENQLLFETAGTPYAKRIKGIVAIYERVAKAVSETLKNKDFPIVLAGDHSTAG
                     GTVAGIKMTYPKSKLGVIWIDAHADIHSPYTTPTGNVHGMPMAANLGEDNLANKINKP
                     DKETVDLWTKLKKIGGMSPKITYKDIVYIAVRDIEPQEAYLLKKHNVKMISAAEVKRI
                     AVEKIVRTALAHLSHCTHIYVSFDVDSMDSSISKGTGTPVRNGISERDAGKLCVRLVQ
                     NEKVCCFEICEVNPTLDKENLMAENTFEILQRVVNQINH"
     gene            45708..47102
                     /locus_tag="PKX59_01295"
     CDS             45708..47102
                     /locus_tag="PKX59_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HOZ89529.1"
                     /translation="MKNRYIDLIQQSYDFPQEGFEVIDDSLHFNGINLMDVIKQYGTP
                     LKITYLPKISSQIQKAKRLFKVAMAKVDYKGSYYYCYCTKSSHFQFVMEEALKNDIHI
                     ETSSAFDIPIIRSLYESQKINKDIYILCNGFKREGYIEGIVNLIKDGFTNVIPILDNV
                     DELQEYEKAMPGKKFKIGIRIASEEEPNAEFYTSRLGIRQRDIVDFYKNTIKKNPNVE
                     LKLLHFFINTGIRDTAYYWNELQRNIAFYADLKKICPELTALDIGGGFPIKTKLEFNF
                     DYQYMADEIVMQIKSACDQRKVPVPHLFTEFGSFTVGESGAILYSVTGIKQQNDKELW
                     YMIDSSFITTLPDTWGIKQKFILLAINHWDKEQQRVNLGGITCDGEDYYIMESVKNRV
                     FMPRPTPDEPLYLGFFHTGAYQDSLGGYGGIQHCLLPAPKHVLIDKTEEGELSMWLFA
                     KEQSSKSMMKILGY"
     gene            complement(47155..47673)
                     /locus_tag="PKX59_01300"
     CDS             complement(47155..47673)
                     /locus_tag="PKX59_01300"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HOZ89530.1"
                     /translation="MKRNLIGVVFSFIILTGYVSCSQPSKIEHKIKWMDFESAVAASQ
                     KNPKKLFIDVYTDWCGWCKKMDASTFMDDSVAAFINANYYPVKFNAESKDTIHFFDQV
                     FVFVPEYKSHELAISLLNRQMGYPSYAFLDEKFAAIKVQQGYMPVETLMPLLKYYAHD
                     AYKTKTFEEFTK"
     gene            complement(47690..49756)
                     /locus_tag="PKX59_01305"
     CDS             complement(47690..49756)
                     /locus_tag="PKX59_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOZ89531.1"
                     /translation="MQPQKEFKPYISATDSMAEFTLKSILLGSMFGIIFGASTVYLAL
                     KAGLTVSASIPIAVLAISIGRKFFKTTILENNIIQTTGSAGESIASGVVFTLPAFLFL
                     SVNESGISNGIQYFEYWTIFTLAVLGGMLGTLMMIPLRRSLIVKEHETLPYPEGTACA
                     QVLIAGEKGGDFARTAYLGLGFAMLYAIFQKVFHVIAEVPAFVTKQTNKYLPSATVSG
                     EITPEYLGVGYIIGPKIGGVLVAGGVLAWLGLIPLLATLVPTDMIAAQLVKLGYLKDV
                     LTASGPGGWNPETHTFTDMADAIYRAYVRQIGAGAVAAGGFITLLKTIPTIISSFKES
                     VSSLKSGDKEQKVSRTENDLSFKTVIVGSVVLIILMALLPQVPGESFLNKLLIGLLIV
                     VFGFFFVTVSSRIVGIIGSSNNPISGMTIATIMGTALVFIAIGWTGKVFEPMALVVGG
                     LVCIAAANAGATSQDLKTGYIVGATPRYQQMALFIGAIVSSVVIGFTVKYLDTPTADL
                     TAQGIHHAIGEKYAAPQATLMATLIKGLLSYNLDWQFVLVGVFIAITMELCGVKALSF
                     AVGAYLPLSTTLPIFAGGMVKGIVDWNAKRKHKKAEGDDELGKGNLFATGLVAGGALA
                     GVVVALLSVDEGVYAALEKVNMEHSIAAVIGEDGYQLLGVGFFTFMSVILYRIAIKPE
                     KKTLNN"
     gene            complement(49825..51264)
                     /locus_tag="PKX59_01310"
     CDS             complement(49825..51264)
                     /locus_tag="PKX59_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ89532.1"
                     /translation="MFYADVWNINHHQMKSSSNAGALATIITVFFFWGFVAASNGIFI
                     PFCKSHFNLSQFQSQLIDTAFYAAYFIGSLLLWFSSQLFRVDILNKLGYKQAVIAGLL
                     ISVAGSLLMIPAVGSGSFVFILSAFFVIALGFSLQQTAAQPFVTALGTAETGAHRLNL
                     GGGINSFGTLLGPILVSLVLFGNLSPEAAASATVESVDTVYYILTGVFLLAALLLAVA
                     KLPKVHSTEKFEPGLGALKFSQLRWGMLAIFVYVGVEVTIQSNMGALLALPQFGGLTT
                     AQISPYISLYWGSLMIGRWTGAISAFNFKPSTKKLLMIVLPIVAYIIVLLANYLRGNE
                     ITNLLPYSLCVAALIAGSFLAQEKPARMLFIFSAGGILAMLIGLFTEGQVAMFAFISG
                     GLCCSIGWPCIFALSITGLGKFTGQGSAFLIMMILGGALIPPLQGYLADVTDIHSSYW
                     ITVFCFAYLAWFAIKVKVILQKQGFHTNQ"
     gene            complement(51320..53677)
                     /locus_tag="PKX59_01315"
     CDS             complement(51320..53677)
                     /locus_tag="PKX59_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOZ89533.1"
                     /translation="MWKFLANKILRNRIAFIVVLLLLTVFMAYKALNIKLSYEFVRVL
                     PTTDPAYVEYENFKQKFGEDGSVMVIGFADSTIFQIEKFNSWFLLSDKIRKMDGIQNV
                     LSIGDLYDVVRNDSLHKFDFVPLVKTLPQRQSEIDSIKNRISELPFYDGLILNRETHA
                     TVMAITFHKKDLDSKHRIDIVNEIKKEADAFGRLHNIELHFSGMPYIRTAVMEKVSHE
                     LKLFLILAVLITAFILWIFFRTISSVVFSILVVAIGVVWSMGSIELFNFKITILTALI
                     PPLIMVIGVPNCIFLINKYHSEYARHGNRIKALARMISTVGISLFLANITTAIGFGVL
                     VFTNSSFLTQFGAIAAINVMVTYFITLIFIPIILSFLPPPSVRQMRHLEGKRMRKILE
                     WVDFIVHHKRNQIYTVIVTVTLLAIYGMTKIDVTGYVVDDLPKKNPVYTDMHFFEKNF
                     KGVLPFEIFVDTKKPNGVFDNNGRVLYKMKALQKQLEKYPELSKALSITEALKFSYQA
                     YRGGDPKYYVLPGISELKNLNDYTSTVKGNENRLKSFIDSSRQYTRISMQMADVGSKK
                     IKTLLADIQPKVDSIFDSSQYQVKLTGHSLMFLKGNDYLLYNLYESLIIEIILIAIVG
                     MALFRSIGIILLSKLPCLIPLVITAGIMGFLDIRFKPSTILIFSIAFGISSDGTIYFL
                     SKYRQELKQHKKSIGEAISATIMDTGFSMIYTAVILFFGFGIFAASDFGGTAALGLLI
                     SITLLVAMITNLILLPSLLLSIDKWVSRKEIVSEPLIELYDNEEE"
     gene            complement(53724..54533)
                     /locus_tag="PKX59_01320"
     CDS             complement(53724..54533)
                     /locus_tag="PKX59_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89534.1"
                     /translation="MQKIIAVCAAFLISVNCFAGEKDCHCTPLFKHGKGTFYLTWGYN
                     LDWYSKSDLHFKGNDFGGYDFTLYDAKAHDKPGLKYIFGSAISIPQYVYRAGYFFGDK
                     HDLGVEISFDHTKYIMTNNQNLHLKGNINGTFYDKDTLVGKDFLMFEHTNGANFLMAT
                     LMKRYCILSSASKNHRLQLILKAGGGLVIPRTDVTIFGNRLDNKFHIAGYVFGIDAGF
                     RYDLMKYFFVETSLKGAFANFTNVLTVGDGRAHHHFFAGEYIFAAGFQIGL"
     gene            54627..55763
                     /gene="mqnE"
                     /locus_tag="PKX59_01325"
     CDS             54627..55763
                     /gene="mqnE"
                     /locus_tag="PKX59_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HOZ89535.1"
                     /translation="MSDIILKSSVDPLLKAIAQKVINEERITPEEGIILYEKGELGFL
                     GSLATLVRNRKNKNFTYFNKNIHIEPTNVCIFSCRFCSYSKDYKHREDGWELTIDEML
                     NKVRAYPSGSITEVHVVGGVHPKMDLHYFAGLLKEIKNINPSLHIKAFTAVELDYMFR
                     KAKMTNAQGLQYLKDHGLDSIPGGGAEIFDEEIRNKICADKCNAEEWLNIHQEAHQLN
                     IPSNATMLYGHYESYSHRVDHMNRLRNLQDKTKGFQTFIPLKFRNKDNEMSHLSETTQ
                     VEDLKNYAVARIFLDNFQHVKAYWPMIGRATAQLSQNFGVDDMDGTIDDTTRIYTMAG
                     SEEQNPTLTTQELVDLISQNGFVAVERDTLYNHLKVYENVPVAN"
     gene            55774..57036
                     /locus_tag="PKX59_01330"
     CDS             55774..57036
                     /locus_tag="PKX59_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HOZ89536.1"
                     /translation="MQNNTEPLKNNITIVGAGPAGATASMFLSSFGIKHTLIDKAVFP
                     RDKICGDALSGKVLPVLKKLNPDLIQQLLVQNEKCLGSNGILFGAPNGKTLEVPFKAV
                     NSTLNHSPGYVSKRIDFDFSLIKLLNPEYCNFLQGHEVKKITKGEEGVTLNVLNTQNK
                     ETYNVSSSLVIACDGAHSICNKLFPDHKLDAGHYCGGVRAYYSGVTGFHSENFIELHF
                     LKEMLPGYFWIFPLPNGMANVGAGMLTRTISSKKVNLKEAMLLAIKNNPSIKDRFKDA
                     NIIGNIDGWGLPLGSKKRKLSGNRIILCGDAASMIDPFSGEGISNAMYYGMRAAEVSR
                     DAINSSNYTDDFLSGYDQFVYNRLWNELKLSHTLQKLCNYPSLFNFVVNKASKNETFR
                     QTISCMFDDIDLREKLRKPSFYFKMLFG"
     gene            complement(57101..58363)
                     /gene="metK"
                     /locus_tag="PKX59_01335"
     CDS             complement(57101..58363)
                     /gene="metK"
                     /locus_tag="PKX59_01335"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOZ89537.1"
                     /translation="MPYLFTSESVSEGHPDKVADQISDALIDNFLAYDPTSKVACETL
                     VTTGQVVLAGEVKSNAYLDVQDIARKVIKEIGYTKSEYMFEAHSCGVFSAIHEQSTDI
                     NQGVERKKPEEQGAGDQGMMFGYACRETDTYMPLAIELAHLLLRELAAIRREGKVMKY
                     LRPDAKSQVTIEYSDDNKPQRIDAIVISTQHDDFAKEAAMQKQIKQDVINILIPRILK
                     KLPKRVQVLFNDKIKYHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSG
                     KDPSKVDRSAAYATRHIAKHLVAAGVCDEVLVQVAYAIGVAQPVGLYVNTYGTSKVKM
                     TDGEISNKLSKMKEFDMRPYFIEKRFNLRSPIYLETAAYGHMGRDSKTVEKVYNKGQA
                     NEKKIKVKLFPWEELNAVNETKKVFGIK"
     gene            complement(58579..59454)
                     /locus_tag="PKX59_01340"
     CDS             complement(58579..59454)
                     /locus_tag="PKX59_01340"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOZ89538.1"
                     /translation="MKALFTIWLCLVFSLTAFSQNEYNNFVVERKNVQKQIDEMLRSD
                     KAFKNRNATAEFQALIRKLLELDDKIILSANYTVNTLQNENKDLEKQANLKPKTIIKT
                     VHGKTVTVANDSIYDALFIAQNRLTNLNDALKAKDGAFDQLLNKSDSLIVANKTMQAR
                     FDALMADNKALNEKNIVLIVFNSLVAVGLILSLFFLLRKPVAKKMLLNTPESEKPLAR
                     VEHSRVLQQVPVAKPVEVKPEPVIKVSPEGIFSASHDALDFKLDQIEKLARLKEKGYL
                     TDEEFNIQKRQILGV"
     gene            complement(59481..61520)
                     /gene="paaZ"
                     /locus_tag="PKX59_01345"
     CDS             complement(59481..61520)
                     /gene="paaZ"
                     /locus_tag="PKX59_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014244095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HOZ89539.1"
                     /translation="MRLKNYIAGQWVEGTGSGTPLFHAVTGEQIAIASSEGLDFKAML
                     DYARTVGGPALRAMTFHERARKLKALAMHLMAKKEMFYQLSGATGATRNDSWIDIEGG
                     IGTLFAYASKGRRELPDETFLVEGNIEPLSKNGTFIGQHICVPLEGVAIHINAFNFPC
                     WGMLEKLAPTIVGGMPAIIKPATATSYLTELMVKEIIASGILPAGALQLICGSAGDIL
                     DHVNCQDIVTFTGSAETGRMLRQKPAIIDNSVRFTMEADSLNCCILGQDASPDTEEFK
                     LFIKEVAREMTAKSGQKCTAIRRTIVPAKYADEVATALKKRLTDIRLGNPSVEGVRMG
                     PLASKSQVKDVTAQVAKLMTVCDVAFGDLKNFEVTGADKEKGAFMPSMLLYCKKPLST
                     AEPHAIEPFGPVTTIMPYDNTGEAITLAKMGKGSLVGSLFTADDNIARQVVLNTAAYH
                     GRMLVINRHCAAESTGHGSPMPQLIHGGPGRAGGGEEMGGIRGVKHFMQRTALQGSPT
                     TLSNICNTYLKGAETFEDIIHPFRKYFDDLKIGDTLITHKRTITEADIVNFAGISGDN
                     FYAHTDETSLEGSIFERRVAHGYFIISAAAGLFVDPKKGPVLANYGLDSLRFTQPVYV
                     GDTIQVRLTCKSKTKKEDREGQVPQGVVEWDVEVTNQRGETVAVETVLTLVARKM"
     gene            61784..>61895
                     /locus_tag="PKX59_01350"
     CDS             61784..>61895
                     /locus_tag="PKX59_01350"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89540.1"
                     /translation="MLGWEFPPVINGGLGVACHDLCLAMVKHAKVSMVIPK"
     assembly_gap    61896..61980
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <61981..>62208
                     /locus_tag="PKX59_01355"
     CDS             <61981..>62208
                     /locus_tag="PKX59_01355"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89541.1"
                     /translation="LKQVNYKEEYKTFEDVHFIPSALNPYYTDRKAAFEIPGIDVESQ
                     YMKEVKIGNRHAKVFHIDDLYGGDVIEKVTVF"
     assembly_gap    62209..62245
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <62246..63099
                     /locus_tag="PKX59_01360"
     CDS             <62246..63099
                     /locus_tag="PKX59_01360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOZ89542.1"
                     /translation="LTLDFDIIHAHDWMTMLAGMEIKKQSGKPLVVHVHSLEVDRGGV
                     NSQGWVYQMEKRGMQEADVLMPVSNFTGNIINEYYGIDYRKIFPVHNGIREVKPFRSK
                     SPYKEKIVLFVGRLTRQKGPEYFVDIAAKVLEKNQNVRFIMAGTGDAQSRILEKVAQN
                     RIGHRFHLAGFLGLDKVRYLLSIADAYCMPSVSEPFGLSAVEAVQFDVPVVISKQSGV
                     SEVLSGALKFDFWDINQAAEYLLGLLDNDTLHKQVVKDAQKDLEDISWDIAANKVIEG
                     YKKYKLI"
     gene            63163..63543
                     /gene="gcvH"
                     /locus_tag="PKX59_01365"
     CDS             63163..63543
                     /gene="gcvH"
                     /locus_tag="PKX59_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOZ89543.1"
                     /translation="MNLPADLKYTKEHEWIKIDGDIATIGITDFAQGELGDIVYVDIN
                     TLNETLKKDEVFGTVEAVKTVSDLFMPVDGTIVEINPKLTDAPDSVNKDPYGDGWMVK
                     VKISDTSQLSALLSADGYAAIAGA"
     gene            63580..63948
                     /locus_tag="PKX59_01370"
     CDS             63580..63948
                     /locus_tag="PKX59_01370"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOZ89544.1"
                     /translation="MWGLFIFILCAIPGKNLPHYSWADLLSVDKLVHFTLFFILILLL
                     KRAWLFIYKGVSSYNLIAIAIGVIYGGSLELMQSYFYTDRSGSWFDFLANSLGAICGV
                     LFFEKLIDKSGRWKFNLGNK"
     gene            complement(63949..64809)
                     /locus_tag="PKX59_01375"
     CDS             complement(63949..64809)
                     /locus_tag="PKX59_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ89545.1"
                     /translation="MVSDFYYEQKLFKAARAIAMVKGPSEVLDETYFEPLFDQYPVGD
                     PVMYDAVSIRERGIQRAEDLIGIMHNYTRTSYNNILELGAGDGMVSSSLQERGKQVTA
                     IEFRDDDFDQRAQESGVNLMKMDAAELQFEDHSFDFVFSYNCFEHFPDPQKVFEESLR
                     VLKPGGLMFIHFAPLYHSPYGAHAYNNVGIPYCQFLFDKHFLNKWINTHADEKFNIDA
                     TLNRWHIDQFRKLFKQTYWDKCKIGYYKEFRSPYFLDLVIKHPQCFKNKIMSMDDLII
                     SEIKAVFVKQ"
     gene            65266..65517
                     /locus_tag="PKX59_01380"
     CDS             65266..65517
                     /locus_tag="PKX59_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOZ89546.1"
                     /translation="MNREEIVATTKRFLSEEFEVDETLIEDQKNLKDTLELDSLDYVD
                     LVVVIEENFGFKTSGEDFKSISTFEDFYNFVERKLAAMA"
     gene            65522..66391
                     /locus_tag="PKX59_01385"
     CDS             65522..66391
                     /locus_tag="PKX59_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOZ89547.1"
                     /translation="MAQWDGRSRGTVLGYRIFVFMIQYGGVRAAYFLLRFVTYYYFLF
                     AAKPKKILINFYQRALKVNEVVAKKLCRKNFYYLGQTLVDRTAFLLGKGNVFSHNFEN
                     EKFLVEMQEQRKGGILISAHVGNWETAGNLLKSRVSSTIHVLMVDAEAKKLKQYLDNS
                     TGGSQFSIIAVKDDLSHIVKVNNALAANELIAMHGDRYLPGTKTIQLDFMGRKAHFPY
                     GPFLLASRFNAPVTFVFALKANDFHYELSATKPITEKKSPEDIAALYVEELEKKVKKY
                     PEQWFNYYDFYSE"
     gene            66388..66804
                     /locus_tag="PKX59_01390"
     CDS             66388..66804
                     /locus_tag="PKX59_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89548.1"
                     /translation="MIIEKVNIENYIPQRFPFVMIDNLIHANESGFLSSFLVKKENIF
                     SENGYLTEPALIENIAQTCAAGFGFIGQQKNEGPSIGYIGSISKLTVHQLPEYGTTIL
                     TTVEITNTLGNIFLIKGKNTCNEKLLVECEMKIALA"
     gene            66801..67229
                     /locus_tag="PKX59_01395"
     CDS             66801..67229
                     /locus_tag="PKX59_01395"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOZ89549.1"
                     /translation="MKQLLQVEIPIKVRFSDTDAMGVVWHGNYLRFFEDAREAFGIKY
                     GLTYLDVYHRGFFTPIVKSSVEHKSPINYGEEVVAIAKFHPTPSAKIMFDFDILNKTT
                     GKLSAKGHTTQVFLSVVEREMELVKPPFYIEWENSIGIKH"
     gene            67233..68309
                     /locus_tag="PKX59_01400"
     CDS             67233..68309
                     /locus_tag="PKX59_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOZ89550.1"
                     /translation="MIYIGSETILSPNGNVVDCFKKIAEKKSGVSKIPGNGFKGESFY
                     CSMYLENRPDFNTLAAQTIEQLLQKSKANLLTEESTLLIISTTKGDLSKGFENAIISP
                     AKFLYEKLKLKHFPLIISNACASGVMAINAAAAFIENKQYQNIIVLGIDMITDFIVYG
                     FQSLYAISDEHCKPFDNDRKGINLGEGAGAVLLSASKDVFTSTPVCFLGGSSSNDANH
                     ISGPSRTGEGLFRSINKTFNSAGIKTEDIDFISAHGTATVFNDEMEAIAFNRLNMQHI
                     PVNSFKGFTGHTLGAAGVIETALCITCMHENKLLPSAGYYNKGTTVELNLIKDAASAT
                     INTVLKTASGFGGCNASLLLQKIK"
     gene            68306..68917
                     /locus_tag="PKX59_01405"
     CDS             68306..68917
                     /locus_tag="PKX59_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89551.1"
                     /translation="MKITGCCRIHQNEVLVNGKKYFTSDKNQHEELSMQLYRFLKTEY
                     PKFYKMDLLSKMGWLATEIVCQTIPTISKFADDEIALLFANSHSCSESDERFNHSFKV
                     ENSPSPSQFVYTLPNILLGELAIRHKWYGENLFTILPEFSAEHFSSHAGIFIPQKAKA
                     LFCGWINLFPVTDVFVFFAEANSETSPGILDSKEIEHWYKIIK"
     gene            68919..69176
                     /locus_tag="PKX59_01410"
     CDS             68919..69176
                     /locus_tag="PKX59_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOZ89552.1"
                     /translation="MDNLKEELKKHLITQLNLEDKTPADISDEAPLFGDSGLGLDSID
                     ALELIVLLDKHYHIKLTNPEEGKSVFYSISTMAEYIEKNKK"
     gene            69183..70361
                     /locus_tag="PKX59_01415"
     CDS             69183..70361
                     /locus_tag="PKX59_01415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOZ89553.1"
                     /translation="MKVYVTGIGVVSAIGLTVEENYHSLLNKKSGITAFKFPEEQKSI
                     QAGSIAKSNAELKQMLSITNGNFSRTSLLGIIAAEQAMGKNFHNKKLRTGFISSTSVG
                     GMDKTEKYYRQVLKNLPVDFNLTRTHDSGNTSENIAAYLGFSGYINTLSTACSSAANA
                     IMLAARMIEQGKMDRVLVGGTDPITDFTIKGFQSLMIYDDEWCKPFDHNRNGLNLGEG
                     AGFLLLESDHSINISGNEPICSLTGWHNAADAYHQTASSPDGKGATIAMKNALKKAGL
                     GPKEISYINAHGTGTKNNDLSESIAIKNVFGESIPPFSSTKAYTGHTLAAAGAIEAVY
                     SVLAIKNQIHFAGLNYNDPLEETLMHPVTEPTKAVTKHVMSNSFGFGGNNSSLIFSGI
                     "
     gene            70364..71329
                     /locus_tag="PKX59_01420"
     CDS             70364..71329
                     /locus_tag="PKX59_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015811463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HOZ89554.1"
                     /translation="MYCIQSASTISHQPSFASKGFSSNLSKEIESLIKPDYKEYVPPA
                     LIRRMSEILRMSVACSNLALKEAGIEQPDAIITGTGLGCLYDTEKFLNNVITIEGGLL
                     PPTSFIQSTHNTIAGQISLVLGNHQYNMTHTQNSLSFEHALLDTMLMLDEKNGNILIG
                     SADEDIEALHPIAAHFKLNSLPLTSGASFFVLSKNKNEKTLAVIEACHTSAFYSSLEN
                     EIKDFMSTSTDKQIDFILNDGRNKDITRLIETLFPDKCVINYSEICGLYATNSAFAMH
                     LATDIICNKTKQVYGKTIANHKRALIINTLLPARAGFIMIKSNEA"
     gene            71319..72071
                     /locus_tag="PKX59_01425"
     CDS             71319..72071
                     /locus_tag="PKX59_01425"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOZ89555.1"
                     /translation="MKHKIVTVIFILCIIALFVFLYQDKSFFYLLAALIFLYLTITAL
                     GSYQIKWNYFIKSVNTGKPEGISLTFDDGPDPKVTKKILDVLELAGAKATFFVIGKKA
                     EQYPDIINLIVSKGHTIGNHSWSHHAGIGMFSKQKLLADFNQCSQKIEELTGQKPVYL
                     RPPFGVTNPRYEYVLKNTGLKSIGWSIRSYDTSAKNEQKLLHRITSQLKPGAIVLLHD
                     TKDITLAILPALLNYCNQNNIAIKPLAELIEI"
     gene            72068..72691
                     /locus_tag="PKX59_01430"
     CDS             72068..72691
                     /locus_tag="PKX59_01430"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOZ89556.1"
                     /translation="MRLLIAFILFIWIPVFAKAQQEPLNNPAELTEKLKQASISNNTI
                     KADFIEQRFMSALKEPQTSYGIFYYKKENKLRWEKTKPTNYVFISENNKVRVKENNHE
                     KDVSSYNQVVGRIKDLMLTLVNGDFSNGKQFDPQYFQTKSEYIVKLKPRNKRMSNAFD
                     YIQLSFNKKNLLLEELAFYEKSGDRNIMKFSNQMVNTELNDTLFKNF"
     gene            72693..73046
                     /locus_tag="PKX59_01435"
     CDS             72693..73046
                     /locus_tag="PKX59_01435"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89557.1"
                     /translation="MLKDFYTIINKTSGQNCVYRIKLNPAHEIFKGHFPGQPVTPGVV
                     LIQMTTELTQNAIEQNVKLKQVINSKFLNVMNPEKNPEVTFKISITKDGQLYKVATSA
                     EDEGVCFFKITAIYQ"
     gene            73052..74224
                     /locus_tag="PKX59_01440"
     CDS             73052..74224
                     /locus_tag="PKX59_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HOZ89558.1"
                     /translation="MADIAACVIIPTYNNSKTLKKVIDGVLNFSSGNDVIVINDGSTD
                     NTTEILNQYNEQIVLLNNKTNSGKGYSLRKGFKEAISRGFKNAITIDSDGQHYPEDID
                     VMIENALKFPEALLMGSRNMNQESVPGKSSFGNKFSNFWFWVETGQKLPDTQTGFRLY
                     PLEPLKKIKLYTTKFETEIEVIVKLAWQDVKIIPVNIRVLYDMKERVSHFRPFRDFTR
                     ISILNTYLVTLTLFYYLPLRLFKYIRRKGIFTIIKEEIFRRDESILKKSLSVGFGVFM
                     GIVPIWGFQLLVGIPLAVLFRLNKVLFITAANISIPPMIPLIIYFSYYLGGFFVSNDM
                     KFQSMADITLQSIHANFMQYATGACVLALISGIFFMTITYITFLALKFKDKLTEKA"
     gene            74529..75050
                     /gene="azu"
                     /locus_tag="PKX59_01445"
     CDS             74529..75050
                     /gene="azu"
                     /locus_tag="PKX59_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HOZ89559.1"
                     /translation="MRKSLLALASAAFIFAACNQSKQPETPATETTPPAATEEAAPAV
                     APPTEPAIVEISGGDDMKFDKTEITVKEGQTVKLTLKHTGKSPKDAMGHNFVLLASGT
                     SLQDFATAAIKAKDNDYIPADMASSVIAHTKTIGGGETTEIEFTAPSKGTYDFLCSFP
                     GHYGTMKGHFIVE"
     gene            complement(75176..75400)
                     /locus_tag="PKX59_01450"
     CDS             complement(75176..75400)
                     /locus_tag="PKX59_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOZ89560.1"
                     /translation="MENKKHLQFKTNINCSGCVAAVKPHLDKLISSESWHVDTANKDK
                     ILSVSTNDISEEEIINTVQKAGYKIELLKK"
     gene            complement(75502..77916)
                     /locus_tag="PKX59_01455"
     CDS             complement(75502..77916)
                     /locus_tag="PKX59_01455"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOZ89561.1"
                     /translation="MEKNTDDTIFLPLENVESEHCALIVDKGLSKVAGVDSHKVELNN
                     RRAVITVSNKETIKDVIATIKDLGYGVTTVRKKFPVLNMSCASCAVSAESMIQSVNGV
                     INASVNFATTELTVEYLPNMTNPLALQKALQEVGYDLLIEDEASQQNSFENIQQEKFK
                     KLKQKTVLAIALSIPVVIIGMFLMDIPYANEIMWLFSTPVVLWLGKDFFINAWKQAKH
                     RSANMDTLVALSTGIAYLFSVFNMLFPQFWHQRGLHAHVYFEAAAVIVAFILLGKMLE
                     EKAKGNTSSAIKKLMGLQPKTVIVIQSDGTEKQTFVEDVIVDDIILVKPGEKIAVDGK
                     LINGSSYVDESTLTGEPVPVLKNINDNVFAGTINQKGSFQFNALKVGKETMLAQIIKT
                     VQDAQGSKAPVQKLVDKIAGIFVPVVIGISILTFVLWQIFGGDNGLVQGLLAAVTVLV
                     IACPCALGLATPTAIMVGVGKAAENGILIKDAESLELAKKITTVVLDKTGTITEGKPV
                     VTDVYWYNDNSSTASILYSIEKKSEHPLADAVVKNFTKTEDIELTSFESITGKGVRAT
                     YQKENYFVGNMTFIKEQNILIEDMKVEKATAWTKESKTVIWFADSKQVLSIIAISDSI
                     KPTSVAAIQQLHKMGIELYMLTGDNESTAKAIAEKTGIDKYQASMLPHQKADFIKVLQ
                     QQGKVVAMVGDGINDSTALATADVSIAMGKGSDIAMDVAKMTIVSSDLNKIPMAIRLS
                     KQTVATIRQNLFWAFIYNVIGIPIAAGILYPINGFLLNPMIAGAAMAMSSVSVVSNSL
                     RLKWKK"
     gene            complement(77997..78515)
                     /locus_tag="PKX59_01460"
     CDS             complement(77997..78515)
                     /locus_tag="PKX59_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOZ89562.1"
                     /translation="MAVQKLLEDSGFITTDVQLGEVTIQVTPDEAEKKQLQDNLKKLG
                     FELIDDKKSRIIEKIKTLIIDLVHHHDGELKKNLSEVLSNELKHDYTYLSNLFSEVEG
                     TTIEKYFIAQKIERVKELLVYDELSLSEIAFRLNYSSVAYLSNQFKKVTGLTPSHFKQ
                     IKADKRKPLDKV"
     gene            complement(78566..79969)
                     /gene="mnmE"
                     /locus_tag="PKX59_01465"
     CDS             complement(78566..79969)
                     /gene="mnmE"
                     /locus_tag="PKX59_01465"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOZ89563.1"
                     /translation="MLQTDTICALITPPGRGTVGVIRISGMKTFEIISKIFTPANKTL
                     STSELEHQRIYFGKIVDKNDLIDEVLVSIFKSPATYTGEDVAEISCHGSGYIRQRILQ
                     LIMQSGAVMAKPGEFTMRAYLNGKMDLAQAEAVADIIEAENCISHHAAMQQMRGGYSE
                     EIKSLRDQLIKFAALIELELDFSEEDVEFASRAELKTLVLNVQSKIEHLLKSFELGNV
                     LKNGVPVVIAGKPNVGKSTLLNALLNEERAIVSDIAGTTRDTIEEELNVDGIRFRFID
                     TAGIRKTDDAIESIGVKRTFAKANEAKTILFLFDPMESSPADLKNEILQLQNELGISD
                     NELQKKVLFVANKSDALSTNFDMHSYDKCGVVILISAKNKSNLNLLKETLIERSGIDG
                     VALNESVVTNARHAISLSQAKSALDLFLQGMEDGISNDLLSTHIRDAIYSLGEITGQV
                     STEDLLDYIFSQFCIGK"
     gene            complement(79969..80799)
                     /locus_tag="PKX59_01470"
     CDS             complement(79969..80799)
                     /locus_tag="PKX59_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOZ89564.1"
                     /translation="MKWAYYDGQWVDAATPVLKLNNRGFIYGDGLFESIRCSRGKANF
                     LSLHIERLLAGVAVLNLKCDFITEDYLREIILEMNQRNNFTDGSKLRLLITRNAGGTY
                     LPEQNNASLCMLPITAVESEYTIPKCFIELGIYSEQQKPINKLSGFKNCNSLLYVLAS
                     IYAQEMKFDDCLILNSKNMLCEATASNVFWLKGNKVYTTPLSSGCVNGVMRKVIIQLT
                     SAIEQDISIEQLIDVDEIFLTNTGSGIRPVNKMQGKVFGTNFGMQLTNRLNNFIIEQK
                     "
     gene            complement(80780..81292)
                     /locus_tag="PKX59_01475"
     CDS             complement(80780..81292)
                     /locus_tag="PKX59_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOZ89565.1"
                     /translation="MINSNKAIFFDRDGVINDNTLYYTYKIADVVFNDGIFDAMKIFQ
                     DNGYLIFVVTNQSGIAKGVYTHNDVLAVHQFMSAEFKKKSITITNYYYCPHHPEKSKC
                     LCRKPDSLLLEKAIARYKVDKNNAHFIGDMETDMIAANRAGVKGHLIESNSNIVELAR
                     KICQHEVGVL"
     gene            complement(81292..81807)
                     /locus_tag="PKX59_01480"
     CDS             complement(81292..81807)
                     /locus_tag="PKX59_01480"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOZ89566.1"
                     /translation="MVSQHKQWYRFITSGFVHADFLHLFLNMFVLYSFGPIVENHFTL
                     INGESGSILFLIYYLMGLVVSILPTYFRNKDNQFYNSLGASGSVSAVLFSFILFFPLQ
                     KIYLYGLLGIPGILAGVGYLWLSSYMDKKGGDNINHNAHFWGAVYGFVFPITFAPGLI
                     LTFFDQLTGML"
BASE COUNT        26575 a        14949 c        14167 g        26116 t
ORIGIN      
        1 acgtaaatta cacttgtcag taagtgaaat tctcaagtag ttatgaattc tgccgaattt
       61 gtctgttagc atcggtttca ctatttttca caatacaaat attacatatt aagaaaatta
      121 aaagagtaat tttagttttc tcatttattt tccattgtaa tttgattttt caaggataaa
      181 actacacaaa tttaattaat aaaatttttc acaaaaaaaa tgggagtatg aaaatactcc
      241 cattctgttt ttataatact tagtactatt aatgcttatg gtttggatcg ctatgatcgt
      301 gtcctttcac atcattactt tcgcacatac gggcaatttc tgtaaaaata gtattggtct
      361 cgcccaattt tttagtgata gattcatcac tctcatttag ctcaataagt ccaaacaatt
      421 ctttgcaggc aactgcaagn nnnnnnnnnn tagcagcatc aatgcctgca ggtgcttttg
      481 attgatgcaa tgcaacagca agttcaatca gttgcttata ctctgcttta attggtttta
      541 aattaccatc cttacttgat tgataagttt cagacataac tttatgaaag ttatttagct
      601 ctgcccatga attcatggtc ttgttggcct gactgtttgc tgtcaaactg aatgaagcaa
      661 gtgcaatgaa taaaagaatc cttttcattt tatttgattt ttgtttatgc aaatttatat
      721 gatttcaact taaaacaaaa cagtttttta tctttacccg atgacgagat ttatctatag
      781 tttattcttt aaactttttg gctggaaaat aaagggaaat ataccctatg aattaaaaaa
      841 atatgtcatg attgtggcac cgcataccag caatattgac ttttttgttg gtttagccac
      901 tagaagtatt cttcgacttg acaccaagta tcttgctaaa aaagagctgt ttaaaccccc
      961 tttgggctgg attttttatg ctttgggcgg atatcctgta gatcgttcca aacctggaaa
     1021 actggtagaa aaagttgccg aaatttataa cagtcatgat cagttctcaa tctgtataac
     1081 tcctgaaggc accagaaaat atgcggctga atggaaaacc gggtttcata aaatagcaat
     1141 ggctgtaaaa gcacctgtgg tgatggtagc atttgattac cgcaccaaaa cggttgtcat
     1201 agcccctcct ttcactcctt ctgaagatac acaaggcgat atagagaaaa tcaaagcctg
     1261 gtaccggcaa tttgaaggaa aaataccgga atttggtgtt aaataaaaaa agaacctaag
     1321 tatttgataa ttcagctaaa agttagatat ttgcagcccc aaaatcaggg aggattctgg
     1381 gataaataaa tttataaatc attaaaaatt aaaaaatgcc aacaaaaatc agattacagc
     1441 ggtttggaaa aaaaggccag ccgttttacc atgtagtggt ggcggattca cgtgctccca
     1501 gagatggcaa gttcattgaa aaaatcggga catacaatcc aaggactatc ccggcaacca
     1561 ttgaccttaa tctcgacaga gcactctatt ggttaggcac aggagcccag ccaacagaaa
     1621 caacccgtgc cattttaagt tacaaaggtg tgttgtacaa gaatcacctt atgggcggag
     1681 taaaaaaagg tgccttaact caggaacagg ccgatgcgaa gtttagcgcc tggatggaaa
     1741 acaaaaacct taaagttctt cagcattcac aaaaagtgca atcatcaaaa gcagatgata
     1801 taaaaagccg cttggttgaa gagtctaaaa aacgtgaggt tatagctaaa gccatagctg
     1861 aaaaacgtgc tgctgctaat gttgccgaag ccgaagctgc tgctgaagca cctgcaactg
     1921 atggaggcga aacaccggca gaaaacgcat aaatattaat tattttataa tgccctttga
     1981 taatgggttt tgacgaggtt acttttttcg gaacaattgt taaaacacac ggtatcaagg
     2041 ggcatttggt tttaagatgc tacgataact ttaagtgcaa aaagaacaac cctgtctctc
     2101 tttttattaa agaaggtgat tttttaaaac aggttgctgt atcagaaatt caattcactt
     2161 atcccttttg cagaataaaa ttttctgatg ttgcagatat aaatgaagct gaaacatggc
     2221 tgaaaaagga agtatatatt gagaaaaaag tgatgcttca aaacgaaatt ccttataagt
     2281 atcaacttat aggatgttct gtcactgata gcatttccgg accaataggt aatattgttg
     2341 acattgcaga atatccacaa caacaaatag ccattgtaaa tgctaatgga aaagaggttt
     2401 taatccctct taataagaat ttcattaacg cattcaatag tactgaaaac aatttagctg
     2461 taacacttcc tgatggtctg cttgacattt atcttcagta gttttatcca tttcacatac
     2521 acatttttat aaaactaaaa ttccgcttaa ttttagctct aactaacatt aaaaattctc
     2581 cttaaaaaca tactaataac ttgacacgtt atttactttt atgactctta ttttttcaga
     2641 tattcgcagc aaattatcgg ggacgaatgg agattaaata tatcggagag cagttaatct
     2701 ggggctacat aggcaatggg ttcatttttg ctgcatttat cagtgctata tatgctgcag
     2761 ttttatatct gacaagtgta agaaatgagg agaaagcaca atgggcaaca ccattagcaa
     2821 gaaagttttt atacctgcat agtatttcat gctttggtat atttttcctt ctgctgtatc
     2881 ttatattcaa tcaccgattt gaatattatt atgtatggca acactccaac atggctatgc
     2941 ccatgtacta tattcttgct tgtatttggg aaggacaaga aggaagtttt ttattgtgga
     3001 tgttgtggca tagcttaatt gcattattta tttcaagaag aaaccgtcat tgggaaagcc
     3061 cggttgtggg aacaattatg atggtgcagg tttttttggg ttcaatgctt ttaggtgttt
     3121 atatattcgg ttataaacta gggtcaaatc cttttacact acttcgcgac aacgaccagt
     3181 ttgccaacct tccttttata aaaatgcctg attatctttc tcgcattgct gatggtcgtg
     3241 gtctgaatcc tttattgcag aattactgga tggtaattca tccacctact ttattcttag
     3301 gttttgcttc tacggtagtt ccgtttgcat ttgcattagg tggactaatg cgcaaaaaca
     3361 ttacagaatg gattaagcct gccctaccct atgcattctt ctccataatg actttaggtg
     3421 caggtattct tatgggtgcg gcatgggctt atgaagcatt aagttttggt ggtttttggg
     3481 catgggaccc tgtagaaaat gcatctcttg taccctggct tgttatggtt ggtgcaggac
     3541 acgtcatgct tgtgtttaac aaacgtggac gggctttgct cagtacctac atactttgta
     3601 ttctctcatt cttgctagta ttgtatagta catttcttac tcgcagcgga atacttggag
     3661 aaacatcagt acatgcattt acagacctag gcatgagtgg tcagcttatt ttttatatgc
     3721 tgttttttgt aattcttgct gtagtgatgc ttgccataaa ctggaaaaag attccaaaag
     3781 aaaactctga tgacagcatt acatcacgtg aattctggat gttcattgga atgttggtac
     3841 tctgcatctc ggcaattcaa attacattta ctaccagtat tcctgtaatc aataaaatat
     3901 tcggttctaa ccttgccccc cctgttgatg taaaagaaca ttttaattcc tggcaaatgc
     3961 ctatagctat gattatagct ttacttatgg cagttggaca attttttaaa tataaagaaa
     4021 gcaaccctaa ggaagtattt aagaaattgt tgttttctct gataatttca ttaattctct
     4081 ctatagttct cattctgttg ttgaagttta acagcgttca ttacatggta ttgttgttta
     4141 ctgctgtttt tgcaatcatt gccaacttcg attattttct tcgtgtttta aaaggaaagt
     4201 tctctttcag cggtgcatca attgctcata tcggattcgc ttttattttg ttgggtacgt
     4261 taatttcaaa tgccaattca agaatcatat ctaagaacag attgaatatt gatttaggaa
     4321 aagattttcc aaacaacgaa aacattatgc tcgctaaagg tgataccctg cagatgggaa
     4381 aatattttgt cacttatagt ggccgcgaaa aaaaaggtca taatatttat tttgatgttg
     4441 aatattataa actgaacaat aagacaggtg agctaaaaaa agcatttagc ctgaagccga
     4501 ttattcagct taatgaaaga atgggaaatt cacctgaacc ggcaacaaag tggtttccgg
     4561 ataaagatat ttatacacac gttacttatg ctaatctgga tgaatacact aaaaatacat
     4621 ccatggatga gtatgataca cctacagagc atgaaatggc tgtgggtgat actttagcta
     4681 cttcaaacag ccttgttgtt ttgaaaaaat tagcaaaaga caatacaggt attgaagtag
     4741 aatcattcga cagttttggt gttacagcaa ttttggaagt gacggatgta aacaaaaaga
     4801 aacatgtggt cacacctcag tttcttatca aagacagaat ggctgtttct aaaaattatt
     4861 ttcttgatga acttggcata aaattcaggt ttacaagcat cagtcctgaa agcggaaaaa
     4921 taaaacttga aatagctgaa aagaaagcga acaaaaaaga ctttattatt atgaaagcaa
     4981 tcatttttcc tcatataaat ttattgtgga ttggttgtat tttaatgctt acaggaactt
     5041 ggatagtttc ggtaaaacga tttaaagaat atgtccgcta caccaccgac aaaggataac
     5101 agaatgacac tgaccattat tggatcaggg aatgttgcct ggttgttggc catattattc
     5161 aaaacttcag gacataagat taatcagatt tacagccgaa ataaaaaaac cggttctgca
     5221 ctttcaaaaa tcacaggtgg caaatttatt gatgacattc aaaaaattga cagcaaatcg
     5281 gatatctata tcattgcaat taacgatgac agcattgccg aagttgtatc caagttacca
     5341 gctttaaaca atatagttgt tcacacttcg ggtactgttg atttgcaaat actaaaaaag
     5401 aaatttaaaa aagccggagt agtttaccct ttgcaatcta ttacctcaga gagtgtttta
     5461 tcagatgaat taactttttg cttggaagca actaacaaca ccgttttaac ttcactgaaa
     5521 caacttgtaa cttctgtaaa ttgcagtttt gttgtgatga actcacaaaa aagagcaaag
     5581 ttgcaccttg cagcagtgct ggtaaataat ttcactaatc atttgtatgc acaggcattt
     5641 agtttactga aaaaaaacag aattgaattt aatcttttga agcctttaat ttcacatacg
     5701 gtaaagagaa taaatggcaa tcctgcttct tttcaaacag gacctgctgt gagaaatgat
     5761 aaaaaaacta ttcagcaaca tttaatgtta ttaaaagata acaagcaact tgcagatttg
     5821 tacaaacaat tgacagcttc aatttataaa tttcacaaaa aatgagtaac gtgaatttta
     5881 aggaaaaatt aaaagacata aaatgcttta tctttgatgt agatggtgtg ctcactaatg
     5941 gcatgctgca tgtcacaaac gatggaaagc ttttacgtgc tatgaatatt aaagatggct
     6001 atgcccttca attagcagta aaaaaaggtt atcgtgttgt aatcatttca ggtggcaaag
     6061 atgagggcgt tgttatccga cttaaaaatt taggtgtatc tgatatctat gttggtatag
     6121 gaaataaact tcagcagtta cttgctgtaa tgcaggaaca tcatcttaca tctgctcagc
     6181 tactttatat gggtgatgat atgcctgatg cagaagcaat gaaaaactgc gctatagcta
     6241 cttgccctta tgatgctgcg ccacaaattc gcgacatttg tcactacata tcacctgtta
     6301 aaggaggtga tggatgtgca cgtgatgtta ttgaacaggt tctgacttta aacaacgatt
     6361 gggaataatg ggatttctaa gacttatcag atttccgaat ttaataatat tggcattagc
     6421 acaatgcctt atttattata tgattgttgt gcctttactt aaaacatcag aatttacacc
     6481 ttcattgcat acatggcaat ttttactagt tgtcttaagt acagtgttta ttgcagcagg
     6541 aggctacacg attaatgact ttttcgataa agatattgat gctgcaaacg gaatcggcaa
     6601 ggttcacgat ttttcaggat ggacattgaa aacgttttat tttgttttct ccatcatggg
     6661 aattgctgtt ggactttatc tgacttatgc attaaagtta agacaatttg ctttaaccta
     6721 tttgctcaca gcagcactac tctattttta ttcagcatca tataaaagac ttcctttggt
     6781 tggaaatttt gttgtggcat ttttaactgc tgttgctgtt tttcttccgg catttgcaga
     6841 ctatgaattg cagtatgcat ttcgtgacat taaacttcct gtcatcaata ataagatgta
     6901 taatcttaga ttaataattg caattacggc agcgtatgca ttttttgcat ttcttatttc
     6961 attagtcaga gaagtaatta aagatatgga agatattgat ggcgatagag agtttggttg
     7021 caacaccctc cctattgttg caggaatcaa taatgcaaag ttaatttcta ttggtttgtt
     7081 actcctgatt tttggattga ttttatttct tcagattaaa caatcctggt gggaaagcct
     7141 tcctgtgttt ggatacacta ttctatttgt acaagtgccc ttactcattt tagcagtgaa
     7201 attgtatttc tctgcagaaa aaaaacattt caaagttgcc agcatgattg ctaaaatagt
     7261 tatggttggt ggcatacttt cactacctgt ttttaaatat ttcagtacct aaccatatta
     7321 atttttcaat agctgatgtt attagtctat acacataagt tttcaccacg ccttaaatat
     7381 attttcgatt tagttattgg taaacttatg ggtattagtt ttgaaattac ccataatgta
     7441 gaagagttta aaagttatgc aggcccaaaa tttaattact cagaacatca gattgagaat
     7501 gaaatattca tatattctga aagactttta tttgagaaag gaatagaaga ccaacaattc
     7561 ggttttacag aatgggatga tataaaagca ttttatgcaa cacatccaaa atacagtgtt
     7621 ccattcgatt tgttttctgc atctttttat ttagtgagtc gctatgaaga atatcttcca
     7681 catttacggg attcacatga tcgttataat gaaactgaaa gcattgcata tacaagggga
     7741 ttccttcaga aacctgttgt gaatatttgg gcacagaaat ttaagtccat tattttagaa
     7801 aggtatcctt ctctcaaatc agtcagtcca aagtacaagt atatttcaac tattgacata
     7861 gacaatgctt atgcatactt ggaaaaggga cttatgcgta ctattggagc ttatggaaga
     7921 tcattattca atttcgatgt acctcagatt gttgaacgta ccaaagtatt gacacggctg
     7981 atgcatgatc cttatcatac ttatgagttg atgcatgatt tacataaaag atataagata
     8041 aaggtgattt atttttttct gttaggtgaa tatggagaaa atgataaaaa tgtttctgtt
     8101 gataacagaa attttcaatc actgattcaa tctttggctg actatgccga tgcaggcatc
     8161 catccaagtt atggttccaa tataaaacaa ggccgactgc aaaaagaagt tcagttgctg
     8221 actaaaattc tgaaaagaga agtcaccaaa agccgccaac atttcctgaa aattagatta
     8281 cctgatactt acaggcagct tattagtgtt gacattaaag aggattacac tatgggctat
     8341 gcaggaaata ttggtttcag ggcaggcatt tgctcttcct tttactttta tgatttggac
     8401 caggaagtgc aaacgccact tttgattcat ccgtttgctg ttatggatgc aactttcaga
     8461 tattatctaa aagtaaatcc tgagcaggta attgatctga taaaaccatt agtagaagaa
     8521 gttaaatctg taaatggtac ctttatcagt ttatggcata atgaatcact cagtgaaaat
     8581 catatctgga aaggctatag aaatatttat gaagagttac ttaaaatagc tgctgtctga
     8641 aatggaagtt aagtttcttg aacatattga gattgataaa gcaaaatggg atcaaagtat
     8701 caatcaagct ttaaataaac tgccgtatgc tttctcatgg tatcttgatg cagttagtcc
     8761 caactggcat gcattggtaa gtgatgacta caactttgtg tttcccttaa catggagaaa
     8821 aaagctatgt ttcaattatc tctgccagcc tctgtttaca caacaattag gaatattttc
     8881 ttctcaacct atagattttg caatttcaaa tgcgttttta aatgctattc catctaaatt
     8941 taaactgatt gaaataaata taaattcttt taatccggca gccggaaata aatttattgc
     9001 cgccaaaaga ttaaacttcg aattggaatt aaacagacct tacgaagaaa tcagaaaatt
     9061 ttactctgat aatcaaaagc gaaatattaa taaggcaaaa agaaatgaat taagtttaaa
     9121 tcaacctgat atttccgagg ttataagttt attcagacat gacagaggca tgaaaataga
     9181 acgaatgaaa gatgaaagtt atgagttact aagaatattg tactccactt tgaaacaaaa
     9241 aggattatgt tatttacgag gtgtttctga tggaaatcaa actatttgtg gtggattgtt
     9301 tattcagaca gattatcgaa ttatttttct tttttcagga aatagtgttg ctgggaaatc
     9361 ctcaggcgcc atgtcattat taattgactc tgtaattcag gaaaatgcca ataagaattt
     9421 agttcttgat tttgaaggct ctaatgattt cggactggca cgttattaca gtagttttgg
     9481 ttcgattgaa catgtatatt tacacctgaa aaaaaataca cttccttttc ctgtaagact
     9541 atttaaacga tgattatggt aaacattcta agcaccggca actccttatt acaccatttt
     9601 gtagcagaac tgcgtgatgt tgaaattcag aatgatagga tgcgttttag aagaaatctc
     9661 gaacgtattg gtgaaattgc tgcttacgaa ataagcaaaa ccatgatcta tgaaaacacc
     9721 gatgtgacca cccctttagg cattgccaca gttccattaa tgcaaagcca acccgtattg
     9781 ggtacaattt taagagcagg actaccattg catcaaggac tgttgaatta tttcgatcag
     9841 gcagataatg catttatttc agcttacaga cgtcatcata aagatggtag tttcgatatt
     9901 catcttcagt atctatcatc accgccaatt gccggacgag ttctaatttt aagtgatcct
     9961 atgcttgcca ccggtcagtc attagttgaa acttataagg caatgcttga atcaggacaa
    10021 cctgcacata cacatgtggt agcagctatt gcaagcagac aagggattga atatgttaaa
    10081 gcaaatttac ccgatgattt aaccatttgg gttgccgcag ttgacgagga gctcactgca
    10141 cagtcttaca ttgttcccgg cttgggtgat gccggtgatt tagcatttgg cgaaaaaaag
    10201 tagttcaaac agattatttt actatctttc gttgattaat cagaatatta aaacatgggt
    10261 gagtttgtaa aaattctgat ggttatatta atcagcagtg taaaatttgt tgttggacct
    10321 gcctttgctt actatgacga aaagtatgac ttcacaattt tcgaagcaat agtttatcca
    10381 attatcggtg ggatgcttgg tgtttttgta tttagctttt ttagtgatca ggttgcaaat
    10441 gtttggcatt ggataaaaaa tagaataaaa aaaactgttt cacgcaaaca acctttcact
    10501 gaaccaacaa tagatagcga tggtaaagtt gttgtcaatt acacgtatgt agatgctaaa
    10561 gaaaagccta aaaataaaat ttttaccaaa cgaaacagaa gaattgtaac catctggaaa
    10621 aaatatggtt tatttggaat tgcattactt acccctgtta ttatttcaat accaattggt
    10681 acaatcattg catcaagatt agttcctgat aaaaagaaag tttttttata tatgctgatt
    10741 tctatttcag tctggtcaat tatcatggtc tcttcttttg aactatatca tgcctattct
    10801 ataaaagcat tacaacaaaa agttcttgag ccatgaaaaa gttaaagcac tattcgcatc
    10861 ttattttcga cctcgaccat actttgtggg atacaaatgt taatgctgct caatcactgt
    10921 ctgaaatgta tcatcaatat agtttagaac agtatggcat aatcagcgag caagatttta
    10981 ttcaaaagta tgttgacatt aacaacagaa tgtggactga atattctctt ggtcggatta
    11041 aaaaatctta tctgcgtgca ggacgatttg aactcacttt aaaatacttt ggaatcgtaa
    11101 acccggagtt agttgaaaaa ctttctgatt tttttgttca aaatacaccc ggcaaaaagg
    11161 agttaattcc atttgctaaa gatttattaa atcatgtaca tggcaaatat cagttagcta
    11221 ttattactaa tggattttca gaagctcagc atacaaagtt gcgttcatca tcaatagatc
    11281 atttttttag taagataatt atttccgaag aaattggcta ccacaagcca gaccctagaa
    11341 tatttttgca gacagcagaa ttacttcaga ctccaataaa ggattgcttg atgattgggg
    11401 ataacataga aaccgatatt gccggtgcta ttgcctcagg tatggattgt gcttatctga
    11461 atccggccgg aatcaaacac aattacgaag taacttttga aacgcattgt ctgagtgaac
    11521 tgattaaatg gctttaatgt tatgcaaact tttgaaggca tagtcgtaaa aacaacaggt
    11581 aatattataa cggttcaaac aggaaacgaa aggattcttt gccggataaa aggtaatttt
    11641 aggattaaag acattaacag caccaatccc gtagccgttg gtgattatgt aaaatattat
    11701 tttgaaggcg atgatgcatt gattacagat cttctcgaca gaaagaatta tattatcagg
    11761 aaatcaaaaa aattgagtaa gcaggttcat attttagctg ccaatattga ccatgctttt
    11821 ttggtagcaa cacctgtgct gccaagaaca tcaacagggt ttattgacag atttcttgct
    11881 actgccgaag cctattctat taaagcttcc atcattttca ctaaagcaga tatttacaat
    11941 agagacattt taaactatgt agatgaattg aaaaatcttt attcaggaat tggatattct
    12001 gtttatgtag tttcaagtca gatacccgat tcattaattc cattaaggac acagttaaaa
    12061 gataaaacaa atttattcac cggacactca ggtgttggaa aatcttctct tattaatgtg
    12121 ttaattcccg gtttaaattt aaaggtaaca ccgatttctg aacaacattt aaccggaaaa
    12181 catagtacta catttgcaga aatgcatcaa ctccctgaag gtggatttat aattgacaca
    12241 cccggcatca gagaattcgg cacttacgat tttaacaaaa acgatgtgac acattatttc
    12301 cctgagtttt ttcaactatt accacaatgc aaattcaaca attgtattca cgttaacgaa
    12361 agcggttgtg aagttttagc agcacttgaa cgaggtgaag ttagtccatc gcgctatcat
    12421 aattacctga gtattttaaa taatgaagat atatttcgtt gagcaatgaa agttgtcata
    12481 caaagagtta ttacagcaag tgttgaaatt gaaggagcaa tttattcttc cattcataat
    12541 ggtctgttag tgcttttagg tatttctgtt gatgatgaaa aagaagatgt tgactggctg
    12601 tgcaagaaga ttgttggctt acgaattttc tctgacgaaa atggtttaat gaatctttct
    12661 gtcaaagaaa tcaacgggca aattttacta gtatctcaat ttactcttta tggtgatgct
    12721 aataaaggta accgaccttc atttattcat gcagcgaggc cggaaaaggc aataccatta
    12781 tacgaatatt ttattcagca actaaatatg aatgatattg tagtttgtac gggaaaattt
    12841 ggtgcagaca tgaaagtgtc attgaccaat gatgggcctg ttaccataat tattgattcg
    12901 aaaaaacaaa caaatgacca ttaaagattc tcaacaaatt gttgacgatt ggataaaaaa
    12961 acacggtgtc cgatatttca atgaattaac taaccttgcc ttgctgactg aagaagttgg
    13021 tgaattagca agaataattg caaggaaata cggtgagcaa tcagcaaaaa ataatgaaga
    13081 cagtttagag atggccgatg aaatggctga tattttattt gttttaattt gtcttgccaa
    13141 tcaaaccggt gttgaccttg aacaggcaat gaaaaaaaat ttggaaaaga aaacacatag
    13201 agatgctggg cgtcatttga aaaataaaaa actacagtaa tcagtctctg ttttttctgt
    13261 tgattaaaaa atcaacgcca agaccaattg ctaatttctg acgattaaaa tctaatttat
    13321 tgtcaatatt aaatgaaggt gttaattgaa attttacttt tactaaatta ccaccaagtg
    13381 aacaataaaa gcttggctca taaaaaaaat agttgtcttc tacgatctta tctcctattt
    13441 cgggaatctg atagaaaaca aggtttgaat attgaacatt actaaaacga aatgcaatac
    13501 caaaattaaa tcgttttgta ctgcgataaa ttcccggttg gatataaaag cgcctaacgt
    13561 cagcactgaa aaatttaagt gaatcacgat tcaactttac atcccctcct ccatagcctg
    13621 catagaactc aactcccaaa ttctcatcta tcatattgta atatcccaat gcaaatccaa
    13681 acgacttgcc gctaccgcta tagtctttgt cctttcctgt tccgacaact gctaaaccat
    13741 ctactgttac tcctaaatgt ttcaccggtg aatagcttaa ctgtccatcg aaagattggg
    13801 caacgccatt aaattgtggt aatgcaaaag atgcactgaa ttttgcatca ccaccttttg
    13861 ttaatgatgg catgtttacc tgacttggaa cataataaat tggtgagcaa ccggtgctaa
    13921 aaaatgcaat taaaattatt actatgaaga agtttaaatt cagtttagaa gttttcattt
    13981 ttccggaata attatacagg tcattattag aataaaagtt ttgactaaag taactaatat
    14041 tttataacta tgtattaatt gctgccattt cttgattgat gcttttgcct ataaagtatc
    14101 catctttcaa aatggataaa tctttaattc agacgaagaa aatattttat aaaactataa
    14161 ctttgaagtt tcaattacaa aaacatgtcg catcagaaag cgattcaaac aacttggatt
    14221 agcattgcag gcaatatttt gctagcagta gtgaaatgga ttacaggata ttttggtaat
    14281 tcgtatgcac tgattgcaga tgctattgaa tctacaacag atattttttc atcgttattg
    14341 gttttattgg ggttgaaata tgcaagtcgt cctgctgata aaaaccatcc ttatggtcat
    14401 ggacgtgttg aacctttagt aacatttctt gtggtaggaa ttttagttgc ttcagctaca
    14461 ctaatagctg ttgaaagcat cagaaatatt atgacaccgc atcagacgcc tgaatcttat
    14521 acacttattg tccttgctgt gattattatt tttaaagaat tattttttcg cttcgtaaaa
    14581 aagagaaata gtcaaattaa tagtactgcc ttaaaagcag atgcctggca tcatcgaagc
    14641 gatgctatta cttcgcttgc agcttttatt ggaattgcaa ttgccatttt catgggtaaa
    14701 ggatttgagg ctgctgatga ttgggctgca ttggttgctg ccggttttat tttttacaac
    14761 agctacttga ttttcagacc tgccttaggc gaaattatgg acgaacacct ttacgaagat
    14821 gttgttgaag acataagaaa agtggctgga aaagttgaag gtgtaattga tacagaaaaa
    14881 tgttatgtac gtaaaacagg tataaatttt ttagttgacc ttcacatcac ggttagctca
    14941 catattagtg ttaaagccgg acatcagatt gcacatcact tgaaagatgt gctgattaaa
    15001 cacaaccctc ttatagcaga cgtgctcatc catgttgaac cggatgagca cgtttgaatt
    15061 ttaaaacagg aatgttttct gcttactgac ctaaataagc tttaagcagt ttgctttttg
    15121 aattttggcg aagtgtctta atagcttttt ctttaagctg acgtacacgc tcgcgtgtta
    15181 aatcaaactt ctctcctatt tcttctaatg ataaaccatg ctcgacacca ataccgtaaa
    15241 aaagtttaat aacatctttt tcacgctcag gcagtgtttc taatgaacgg ttgatttcag
    15301 tgtgtaacga ctctgccatg agtgtgctgt cagcataagg tgcatcagga ttggttaata
    15361 catcaagcaa tgtattatct tctccgggta caaaaggtgc atccattgat acgtgacgtc
    15421 ccggcatttt catagtgtat gcaattctgt ctgtatcaat ttctaattca ttagctaatt
    15481 cttctgctgt tggttcgcgc tcaaattttt gttcaagact cgaaaatgct ttttttacct
    15541 tgttggcagc accaatctga ttaagtggta acctaactat acgtgcttgc tctgccaatg
    15601 cctgcatgat tgactgacga atccaccata ctgcatagga aataaattta aaacctcttg
    15661 tttcatcaaa acgttttgcc gctttaatta aaccaagatt tccttcatta atcaagtcgg
    15721 gcaaacttaa accttgcgac tgatactgct tagctaccga aaccacaaat ctcaggttag
    15781 ctctcactaa tttctctaat gctcgctggt ctcctgctct gattttacgg gcaagttcca
    15841 cctccatctg tgctgtaatt aactcctctt taccaatttc ctgtaaatac ttttcaaacg
    15901 atgcgctttc acggtttgta atggatttag taatttttag ctgtctcatc tattttatct
    15961 aatttttatg tgtgtttcaa tgtatgcaat gtaagaacaa aaaacgataa tcatggttta
    16021 aaattgcatt atttttttta aaggcgttgc aaatatacta tgcgtttagc ggcaatgcta
    16081 actttatttt taagcaatgc cacacaatgc tgcttatcaa cacattatct ttgccttttc
    16141 aagtaaaaga ctattttttt aaccgatttt gagagttatt tagcggttat ttatgattaa
    16201 aaattcacct gaaaacagaa aaattatttc aatggttatt catctttaat ccaaatttca
    16261 tcctttgatg caattaagag taactgtaag aaacagcgta taaagtatat ttttcagtag
    16321 ttatatggcc acctttaaac cctaaaaaga taaattagta cctgctaaaa aatatttctt
    16381 ctacattcgt gttttattcg aacagaaatt aaaacaaatt ggcatttaac aacaaccaca
    16441 tcatgataaa aacgaccaat atttttactg cattagtatg tttattgtta gttacttcat
    16501 tgtcagtatt aggacaaaaa ggtaaggtaa atcaagagaa aaaaattacc ggtccaggaa
    16561 aatgtttaat aaaatcagat agtcttttaa caaatgaact taccaaagaa gacctaataa
    16621 aatgggtaga aaatccacct tttattgtga actgtgaaga tagcaagact tacaaactga
    16681 agttttacga atttacaatt ttaaaactga agccttttga aaataagagc tttggtgttg
    16741 gtgatgacag aatgataccg attctcggac gcaaggctat agatgatcta aaagaaggtg
    16801 acacggtgat tctaaaagga gttgacctga ttgaagatgg aacagataaa actacaaaag
    16861 cacctactat cagcattaaa attattgaga agaagcctta aaaatataag ggatgaaaaa
    16921 gccatttccc ttttgaatta atttctaaag caggtaatgg aaattttata aaacgggtta
    16981 cttcaaaaaa taatttcaat gcctttgact ttgtttttat tttagtcaga tcaatatcag
    17041 gtgctatata aatttgtctg tagcgcttga agttgggaat cgggttattt ttatattctg
    17101 tagcgttgtc aaaagctcct gtcatacctt cagcaccaat gcctgcagct atattgagcc
    17161 aggcaggtac taaatttgtt ttctttataa aagaattgat gttagcagac aaccacaatg
    17221 tttgtccgtt ataatcttta aaaagttgct gagtataatt ttcacctaat agatcagggc
    17281 gataatgagc atactttgat tgatgaaaag aaaatttgaa ttctattctt tgctcctgcc
    17341 aaagcaattg ctgtccaata aaaacagatg atccaagtgt gttagctgca atatctcccc
    17401 atgaaaaacc ccaggcagaa gaaaaaccat ctaaggcttc taccgatact aaaaaaatcc
    17461 aaccaagatt gccgcctata agtgtggctt ttttattgtc catgcctgcc cagcaacaaa
    17521 cattgtaacc ttgcttaccc gcaatgtatg aactaaacgt atgacctgtt ttatccatca
    17581 gtaaccattc tccattatca ttgaaaaaat gaaaatggtt catatcatat ccactatacc
    17641 ataactgata taatccggcc atggtgcctg catacaagcc aagtgtagta tatcctgcaa
    17701 tttgaacgcg ctgtttattg taaacaggcg aaggttcaaa gaatggaatt tttcgttgtg
    17761 tgttaacaga atcctgacaa aatgaatttg aaaaaatacc aatcagaaat atcatgacaa
    17821 gccacttgtg tgtgtggtga aatggcaaca atctataaat ggaaattaat ttctttggtt
    17881 tcaactcacg cttctataat cagcagcaaa actaaaagaa atgtaaatga aattgaaaat
    17941 tacaatcaat gtgttttctc gcatataaat tcaaagattt cctttcattt caattaaaaa
    18001 agcttttaac ctattcagag caaattttac atggctgtta tcttctttta cctcagtatt
    18061 ttcacttact tctgctacag gtattgcttt accatttgtt ttcagatatt gtttggctcc
    18121 actcaatgta aaaccttttt cctttactaa atgataaatt atccggaatg attctatatc
    18181 ttttttggta taatgccgtt caccttttaa attttttttc gggtgaagca ttgtaaattc
    18241 tttctcccag tagcgcaata acgaggtgtt tactttaaac atatcagcaa cttcgccaat
    18301 gctgtaatac agtttttcta tatgtaggtc gtcatgctgc atcatcagat gatttttcta
    18361 taattcacaa atctaatcga atgattgatt gtattttgaa gaaatttcca acacctgttc
    18421 atattgtgcc gctgtcaggt cgttgaagaa gaagtttatc gggtttatct tgctgccatt
    18481 tttaataacc tcgtagtggc agtgaggccc tgtgctactt cctgtattac caacgtaacc
    18541 tataatctgt cctctgatta ctttctgccc cggcttaaca ataattttac tcatatgtgc
    18601 ataaagcgtt tcatatccaa atccgtgatt aatggttaca ttattaccgt agcctctacc
    18661 ctctttttca acctttttta cgactccatt gcctgtagaa taaatttctg ttccaattgg
    18721 tgcactaaaa tctattcccg tgtgtaaaat acttgtttta tatatcggat gtatcctata
    18781 accaaaacca gaggcaatcc tagttagttc tttattactg actggctgaa tacccggtat
    18841 agatgccaac aattcttttt tattttttgc gagttcaaaa acttcatcaa acgattttga
    18901 ctgtacatac atttgacgca tcaatttatc caactgcgta gttgtttcaa tcatcagcga
    18961 tgagttgtca taacccttta atgaacgata tttatcaacg cctccataac ctgcctcccg
    19021 cacgctttcg ggtatagggt ctgcttcaaa aatcactctg taaatattgt catcattata
    19081 ctcaatatct ttaagaactt ttgacattaa atccattctt tgctgcaaaa tctcgtattg
    19141 aaggtccatt acagcaattt cgcgtttaag ctgcttttct tttggtgaat ccagaaactg
    19201 ataggctaat gctataatga ttgcactaaa taccatagct gttgccaacc atcccaaaac
    19261 acgtaaaaag cgctttttga aggaaacaac taccttttcg tactttaaag aatgtgtatt
    19321 aaaataatat ttggccttcg ccataatctt taattctatt tttgcacaat tcagcaaaaa
    19381 taaaattttc ctgaaatcta attaaagtca aaaaattata aaaatcagtt acttaaatat
    19441 cttcatttaa acttcttaca ccattttaat aaattacatg acctcttcag aaattcgtaa
    19501 aactttttta gatttttttc agtcgaaaca acataccatc gttccttcag caccaatagt
    19561 tgttaaaggc gaccctacac ttatgtttac caatgcaggg atgaatcagt ttaaagattg
    19621 gtttttagga aatcaacctg caaaatacag acgtgttgca gatacacaaa agtgtttgcg
    19681 tgtttccgga aagcataatg accttgaaga ggtgggtgtt gatacctatc atcataccat
    19741 gtttgaaatg cttggtaact ggagttttgg tgaccctaat gatcctaatg caggctactt
    19801 taaaaaagaa gccattgcat gggcatggga gcttcttact gaggtttata aacttcctaa
    19861 agaccgtttg tatgttaccg ttttcgaagg tgataagaaa gaaaatctgc atttcgatga
    19921 agagtcaaat acttgttgga aacaacacat tgatcattca cggattttaa tgggtaataa
    19981 gaaagataat ttctgggaaa tgggtgacac aggaccatgt ggcccctgtt ccgagataca
    20041 tgttgacttg cgaagtaaag aagaaagaaa acaaacagat ggaaaaattt tagtcaatac
    20101 tggccatcca ttagttattg agatttggaa taacgtattt atggaattca acagaaaagc
    20161 tgacggcaca cttgaaaaat tgcctgccag gcatgttgat acaggtatgg gatttgaacg
    20221 tttgtgtatg gcaatacaac aaaaaaaatc aaattatgac acagatattt ttcaacctac
    20281 cataaattac attgaagcaa tgagtggtat aaaatatggc agtagcgagc aaacagatat
    20341 tgccatgcgc gttttgtctg atcatatcag agctattgca tttaccattg cagatggaca
    20401 acttccgtca aacaataaag caggttatgt tattcgaaga atacttagaa gagccgttcg
    20461 gtattctttt acttatctga attttaaaac tccatttatt catagactga cacctttatt
    20521 ggcagatcag tttaaagaag tttttcctga gctttttgaa cagaaagatt ttgtacaaaa
    20581 agttatcaag gaagaagagc aatctttttt acgcactctt gaaacaggta tcagaagatt
    20641 tgatctatat aacaagtctg ttattgatgg agagtttgca ttcgaattat acgacactta
    20701 tggttttcca attgacctta ctcaattaat tgcacgtgag cgaaacatgc aagttgacat
    20761 gaatggattt aaccaatgtt tagaacagca aaaagaaaga tccagagctg catcagcagt
    20821 tgatgctgaa gactgggtta ttcttgaaaa cagtgaatcg aaattcatcg gatatgacaa
    20881 tacaactgct gaggtaaata ttataaaata cagaaaggta aaatcaaaaa ataaagaaca
    20941 atatcagctt gtcttcaatc agactccttt ttatgcagag agcggtgggc aagttggtga
    21001 tacaggctat atccaaaacg aaaaagagaa aatttttatt accgacacac aaaaagaaaa
    21061 taatcagatc attcacctgt cagataaatt gcctgttgat ttgaaagcag attttattgc
    21121 aacagtcaac aattcgagaa gaagaaaaat aacagccaat cacagtgcaa cacatttaat
    21181 gcatgcagca ttaaagcaga ttttaggtaa gcacgtagta caaaaaggtt cacttgtgaa
    21241 tgatgagcac acacgttttg attttgctca ttttgcaaaa gtttctgatg aagatatagc
    21301 caaaatagaa caattggtaa atgaaaaaat tcgtgagaat atagctttag acgaacgcag
    21361 aaatgtgcct attgaagaag ctaaatcaat gggagctact gcattatttg gcgagaaata
    21421 tggcgactat gttcgagtta tcatatttga tcctgcatac agtatggagc tatgtggagg
    21481 cacacatgtt aatgcaaccg gtgaaatagg cttatttaaa attctaagtg agtcggcagt
    21541 ggctgcaggt gtaagacgta ttgaagcaat aacatcaata gaggctgaaa aatatatcat
    21601 aaatcaattg acacagctaa acacgattaa aacagaacta aaaaatccta aagatattgt
    21661 tataagagta aaggaactat tgtcagaaaa tgaaatactg caaaggcaaa tagacggctt
    21721 tgtgcatgag aaagcacagc tcattaaagc tgaccttcta aaaaatgtaa agttgcagaa
    21781 tggcatcaat gttatagcac agaaaattga cttaactaat gctgatgcaa ttaaaaacat
    21841 ttcttttgaa cttcgtaatc agatacctga ttcatttatt ttattagcct ctgagaatga
    21901 tggcaaagcc atgttatcat tgataatttc agacagtttg gtgcaatcag gcaacttgaa
    21961 tgccacaaca gttatcagaa acatttctaa acacattcaa ggtggcggtg gtggacaacc
    22021 attttatgca actgccggtg gtaaaaatcc tgcaggaatt gatgccgcaa ttgaagaagc
    22081 gttaaagatg atataaaaag agcgaggctg cctgaatggg cagcctcgct cttttatatg
    22141 ttattagaaa ttaatattcc cacatgtcgt gttcaagatt tactatctct gatttaattc
    22201 tctctgcttc aagcaaagca tctaaacctg ttttgtaatc ttcaatacgt ctgttataca
    22261 catttgactc tttaataata tagctgttaa acattcgctt atgaaacacg tcatcaaatg
    22321 ttctgcgttc tgtatcattt tcacgattga atgcttcagc atttgcaagc aactttcttg
    22381 cttccggata ataaatccag aaaagaggtt tttgaatatt accttctctg atatttccat
    22441 attcatcttc ggcaagcatt aggggtgcaa taccaataat acggcactcc ataactgaac
    22501 gctgcttgtc aaagaaccac tcttctttaa tacgataggc aataacttta tcacgtgaaa
    22561 aagtacgctg aatggctgaa tcacgagtta gatatggagg ctccggatct gttatctgaa
    22621 tagtatccat accggctcca ccaattttct tgaattcttc caaggtcatt ggagtagtaa
    22681 actcatcatc agttgtagaa taagcagtta atgaaccctc atcaattgca ttcatcaaca
    22741 cctctatcag actttttctg tcttttgtat cgccttcacg ggggaaacga agtggcagat
    22801 taatcttttg tttaagatct attacctccc aaacacgctt tgcccacaat acatcagctt
    22861 cgcgcagaaa tgaatattcc attactttac gagtaggatt gtgttcttta acataaacac
    22921 catccaatac cgtttgggca tttgtttgtt tgaccataaa tgtcatcaca aacaggaaac
    22981 aaaacaacag gacttttttc atcttaccct ccgtggttta ttgttaaacg tattattgaa
    23041 ttgtaaagtt tacacttgga atacttcttg tgccaccgtc cggaccaaca gctttaatat
    23101 attcaaaata tactttatct ccggcacgtg ctttgcttaa agctgctttc atattagcag
    23161 taataaggtt gttattgctt tccaactcaa tcgggtcttt tccttttgga tataaagaca
    23221 ttttgaattt tgttacttta taataaattt caaagtcgaa attttcaagt tcggcaataa
    23281 cggcactttg cgcaataagc gcatttttct gcattgggcc ttcacgctta ccggcaattt
    23341 ttgcaaccgg atcaggaact cttttaacac gaaacttttg tgctcccata ttgattttct
    23401 taccatccat atcagcaata atagaaatag ttgcttcacc ttgagtggta actttaacaa
    23461 tatagtcggc accggtacct ttcgggtttt ttgttaatga tccaccgcca ccagatattg
    23521 aggctacaac tttattgttt ggtacaccag gaactgaaat cgaaacaggg ttttccggtc
    23581 caatatagaa tacgttcatt ttggtaggag aaacaactgc agcaggtttt gcagcaatat
    23641 attctgcttc aaatgggtat tcttttactt tagttggatc ttcagggtct ttaactttga
    23701 ttactccacc ccatttcttt accccttctg atgatggtcg gtcaacatat ttaccaagtc
    23761 cattttctac aggtaaagat cttccaccaa ctacgatttc tgcatcagaa cttttattaa
    23821 aagcagctaa aaatacatct gctttataat cctgaccggt taatacatag cttgtagggg
    23881 ctacaacttt aggttctaca tctgtaaact ttaaatcctt agcattaatc tgcgccaaca
    23941 agtaattgtt tacttgtgat tctgcagtct ttacatcaga ttgaatttta ctcaacattg
    24001 ccaaagctgc aacaacagga gtatggtaaa aggttgccat ttcccatgtt ttcttctttt
    24061 cttctttcgc cagtttgcta gctggatccg gatctttaag attgattaac tcatcaaatc
    24121 gagactgaaa gtctttctta tccttatccg caacgaaacc aatgagttta accttgtaat
    24181 cttccagttt cttctttagt tctgttgctt taaatccctt gccatcttgt ttatcaccac
    24241 acataattaa agttggtgta tcataatcat catttttctg aatttcacga ggagctggaa
    24301 ttttagcatt cgcttcaagc ttgtcagctt tacggacaat ttcatttttc aaatcttcta
    24361 aatacttata caaatcatca gaagctttat gaacctgagt tgctttttcc tgaaagggtt
    24421 tcgcttttac agcatcagcc tgagcggctg ttgcaaatgc atccattacc tggttattct
    24481 ttttaataac gttattgttg gatgcagtta ttccttcatc aatggttata aaagcattga
    24541 tgatttcttt tgaaacgttg agagcaagca gagctgttaa cacgaggtac atcatgttaa
    24601 tcatcttctg ccgcggtgtc agtttaccac tcgccatagt ctatttctct atatctaaaa
    24661 ttggttggtt ataaaataat ttaattagtc agattacttg tttggattca ggttcattgc
    24721 tgatagcatg tttccataaa cagtattaag agaagacagg tttttagcca atttagaaac
    24781 ttcatcttta tacttcttag tatcatctaa tgactcgttc aagttcgaca tcaattggtt
    24841 aattccgtca taaaacttag atgtttgttt taagtggtta ttagaatctt gcaattgaag
    24901 ttcatataca gcgtttagtg ctccaagatt tttgcctgcc atttgcactt gctcattata
    24961 cattttaacg tcatcactag cagttgacaa agagctcatt gcctgtgcag ccttttgata
    25021 tgaacctgaa agttctgtta cagacttagt agctgcattt acatttttaa cataatcatt
    25081 agtagcaaca gatgcatcag aaagatcagc catttttgct gtattatctc ctaatgactt
    25141 taaacctgtt cctaaactgg cgattaaatc cgggccaatt ttagcatcag ataacatttt
    25201 atctaaagcc tgtgaaacag gatcaatttt tttcttttta tccctgcctt ggcttgggtc
    25261 gtgcattcct gctaactccg gatacactaa cgaccagtct gtttcttcgt gaagtggttc
    25321 aaatgcagag aagaagaaaa tgatggcctc ggtaagaaga cctactgtaa gcattatacc
    25381 tgcaccttcc cagtgctgga ttttaaataa cgcacctgca ataacaatgg ctgcaccaat
    25441 accgtacagc ttggccataa agtttttgta cttttttccc gatgtgaatt cagctaatcc
    25501 catagttgat tttgaaaatt taattggttg atttttaatt tatttatttt ggtgataata
    25561 atcgaaataa tttattctta aaagtcggct ttatcacgac ccaagaatga cataacacaa
    25621 cggaaaccta cataacattt tgcagtatcc tgatactcgt aagaacgtgt gcctgtttgt
    25681 aaaaagtatc ctacatcttt ccatgaacca ccacgaatta cttttctttt taatgcaaca
    25741 ggatcagagt ccttcgcatc ataagaataa tcagggttaa ggtcatgaga gaaattatag
    25801 gcagcttctt cataagcatt acttgtccac tcagaaacgt tacctgacat acagtataaa
    25861 ccatagtcat tgggccaata agatgtagtc tttacagtgt aaaacccgcc atcatccata
    25921 tagttaccac gcattggctt aaagtttcca aggaaacatc cacggctgtt tctgatataa
    25981 ggtccacccc atggataagg agctaaatca tgtccgcctc ttgctgcgta ctcccactcg
    26041 gactctgtag gtaatctaaa atcctgaaca aatgattcac cattttcctg caagtagcta
    26101 tttaataatt gtgttctcca gatacagaaa gcacgcgctt gcttccaggt tacacctaca
    26161 acaggataat cgtcataagc cggatgccag aaatacatgt tagtaacagg atcattgtaa
    26221 gaatatgtat aatcgtgtat ccatgctaat gtatcaggat aaacattgat aatatctttc
    26281 ttaataaata ctgaacgatc agtcatacct tgttcacggt ttgacttttg agctgcttct
    26341 ttaaggtcaa tccagtaata ttcgaaattc aatttacgtg aatcaatttc ttttcttctg
    26401 taaaagcgct cactttccgg taaaaacaac tcagctaagg tttctacgtt ctgttcatct
    26461 tcccatttga ttttcttacg ccaattgatg cgttcgccat attctccttc ttcgataagg
    26521 tgatctccac cgattaatct gtgtgcaagc gagtcgcgaa cccactcaac aaactgacgg
    26581 tactcattgt tggtaatctc tgtattgtcc atataaaatg catgaacaga aacagattta
    26641 ctctgtgcta ccattgcgta aggaacatcc tgatcgcttg gccccatatt atatgcgcca
    26701 gatggtataa acaccatacc gtatggatca gcctgatacc agcgctctcg gttaagagcg
    26761 ccaactaact gtcctttgtt tccgccaccg cagctgctga gtaaaataac tgcaatgatg
    26821 ggaatgaata gttttctcat gtttgccaaa ttagtaactg gagaattatg gatgttaaaa
    26881 cgatttatat ttatttttat tactttaagt ctgtactacg ctgttttatt attctgttta
    26941 tacttcagtt tcaaagataa gttactatta aaattaagtt tcccgaaatt tataaaaacc
    27001 ttacatttct taatacagga attacttttg gtttaatcac ataacaataa cctaacatta
    27061 tttcatgtgt tccgctatga tagcccttta attttgaggt agtaatatca taagagtatc
    27121 ctaatctcag gttttcgata atattaatac ctgccattaa aacaattgca tcctgaagtc
    27181 tgtaagatgc tccaatccaa actttatctt taatatgcag attggcattt atatcagcct
    27241 gtgtttcttt agcatctgac tttataaata aagatggttt taatttcaaa gatggtgtaa
    27301 gatcaaagtc aacacctccc ataaaataaa aatgaggtac aacttctact ttggcatcat
    27361 cggtatattc aatttttcct cctgttaaat gtgtggccga agcacctaca taaaatttat
    27421 ctgatttata atataaacca gcaccaaggt caaacttaga gcctgttcct gattttacca
    27481 atgggtcgtc agggtcaata gcattaaagt cgtttccgta accacgctgt aataaaccgg
    27541 catcaagtcc aatccctaat ttacctgaac ccaggtctaa atggtatgaa tatgccagtt
    27601 tgatgttaag atttttttga ttcaatggct tatcagttaa aactgaaaga ccaagtccgc
    27661 catgtaaaac ttctacaggt gattctactc caaaaacagc agtttttggt ccaccaccaa
    27721 atttatccca ttgatcgcgg tatattaaag taccacaaat tgcttcattg gtacctgcag
    27781 atgccggatt tggatacaag cggttaaaca tgttttgtga aaattgagga tcctgttgtc
    27841 caaaggatac tgatgcggtt gccacagcta cgactgtaaa aagtctcttc atatactatt
    27901 caggttgttg attcgtttaa tattatacgt cttaatttgc ggccctaaag taagcaaaaa
    27961 aacaaattgc aaacaaaaga cactttttta tttcaataaa tgcgttaaaa tcgggcatag
    28021 ttacgacagt ttctttaaag cttgccacca tctttccgga atttcgttgt tacaagcctt
    28081 ctgataatca tcataactgc aaggaactaa ctgatgcctg tctaatttct ttttaatctt
    28141 actcagaggt atttccatcc accaacgtcc cgttacatta cttttcagaa aatgcatttc
    28201 ttcgttgttc tctctcagtg tcaaattata aactgtaaac tgagagcttg tgctatcagg
    28261 aaaatcattc tttctgttgc ttacaccttc cataaaatac cacaccattt gagaaattaa
    28321 tgctgctgtc atttggtttg tatctattga ggctgcatac tcataaagtc caattccttc
    28381 taatttatca ctcatacctg cgtaatacat tatctgacag gccatatcgc ctgttaaacc
    28441 gttaggtgat ggcgttgaag tagctgaggc atcgctgaat ctgatacttg ataaatcaaa
    28501 cgtcataaca tcggcatttc tgatagtcgg ttccatttca atagaatcct gatttacctc
    28561 accaagtctg tatgtatcga aatacaaatt gtccatcata tttacagcat agtcacctac
    28621 aaagtaggtc tgatgtgcaa tattactgta attatagaga tgattgggtt gatccatcac
    28681 aatatttcca agccaactca cagagtccaa agggtctgat ggcatgccta aatcaaagcg
    28741 agcatctaca ccaaccatat ttataatctg atctatcttt ttgtatgctc tgtaatgtgc
    28801 aactgtcaaa tcctgacttg cgccaataat tacaggtata atttttagtt gaagcagtga
    28861 aatgacaatc tcagttaatg ctgcataagt atcttctaca gtcactccta gtctcacatt
    28921 tcctaaatct gccaccggaa caccaacacc atgttttttt aatctataaa acgaccgcct
    28981 aacattatca acaccatttt tagcttcata gtcttttttt gcaccacggg tctcctctac
    29041 tccaactata gcaattttaa tattattcat aaaagggaat cccgatgaac caagattttt
    29101 ttcaacatgc tgccacatac aatcaatagg agcatttgac atttcctgct ctatggattc
    29161 actaatggga ctgaaaaatt cactaatttg catgactacc ttcctgactt agactttgct
    29221 ttactttttg ttttggttga tactgctgcc ttctttttcg ttgttgttga tttttttgcc
    29281 gatgattttg actttgactt ctttgcagca gatgcctttt ctgtctttgt tgctttttct
    29341 tttttcagtg gaacacctga caattttgca tcaatcaact ctccaagata atcaatattt
    29401 acttcgaaag gatcgtctcc ttttttaaga gctacaaaca aatcgtcata tttaatgtag
    29461 gggccaaacc ttcctttggc aatgactaca tctttgcctt tatattgtcc tgcatttcgg
    29521 ggcagacgtg gtctgcttaa aatatctaca accgtttcta aatctatttc caaaggatct
    29581 tcgcctttgg gaagtgatat aaattctgtt ccatatttaa tgtatggtcc aaatcttcct
    29641 ttgttgatta taatatcaag tcctttatgc tgacctaatg tacgtggtaa acggggtccg
    29701 tcaagaattg cctttattga ttctatttca atggtaaaag gatcttcttc tttgggaagt
    29761 gaaatgtatt cttcaccgaa tttgatatat gggccatagc gaccattatt aactatcaac
    29821 tccttgttct tatactggcc gaaatgacga ggcaacttaa ataaatccag aacttcctca
    29881 agtgtaaccg tttcaagtcg ctggtttttt tgcaggctgg catacatagg tgtgtctgat
    29941 tccttttccg ccacaatttc tgccattgga ccaaagcgac ctaagcgaac tatgaccttt
    30001 ctttttgtct ttgggtcttt ccctaatgct ctttcacctg tagctctgtc actcgtctca
    30061 attgtattct ctacattctt atgaaatggt ttgtaaaaac ctgcaatcat tttattccat
    30121 ttcaacttgc cttcagcaat ttcatcaaac tctttttcaa tttcagcagt aaaattataa
    30181 tcaagaatat ctttgaaata ttctaccaga aaatcgttga ctacaatacc tgtatctgta
    30241 gggtacattt tatttttttc tgcaccggta atttcagtct tttcaacttt atttatcttg
    30301 ctgttactaa gttcaagcac agcatatttt ctttctttac cctgacgctc ttctttaata
    30361 acatagcccc tcttttgtat tgtagaaatc gttggtgcat aagttgaggg acgtccaatg
    30421 cctaattctt ctaacttttt caccagactt gcctctgtaa atcgagccgg aggctgtgaa
    30481 aaacgctgcg tggcagtaat agatttataa tctaattttt gcccttcttt taatggtggt
    30541 aataatgtgt cattatcctc ttcgttttca tcatctgatg attctgtata aactttcaga
    30601 aaaccatcaa agatgataac ctcacctgtt gcaacaaatt tctcagtgct tttatctgat
    30661 gcaattttaa caatggtgcg ctctaatttt gcatcagcca tttgtgatgc aatagcacgt
    30721 ttccaaatca aatcgtataa acgctgctca ccgggagtac cctttataac cgaacgattg
    30781 aaacttgtag gtctaatagc ttcgtgtgct tcctgtgcag atgcagactt ggtttgatac
    30841 tttcttttct gatgataatt tttaccataa gacgtactga tttctttttc agcatttgtt
    30901 aaagcttgtt ccgataaatt tacagaatcg gtacgcatat aggtaatcat ccccgactcg
    30961 taaagctttt gagcaatgct cattgtagtt gctacggcat atcctaactt acgtgaagcc
    31021 tcctgttgta aggttgaagt agtaaaaggt gcagcaggtt ttcgtgctga aggttttttc
    31081 tctaatgatg caatattaaa agttgcattt ttatttttct caagaaatgc ttcagcttct
    31141 ttttctgttc cgaatttttt atccaactcg gctttcaata aagttgtatt tcctgattta
    31201 tcttcaacca gaaaattagc tactacctta taaaacgaag taactccaaa atcctgaatc
    31261 tcgcgttcgc gctctacgat taatcgtact gctacagact gtactcttcc tgcagacaat
    31321 gaaggcttta ctttcttcca taatattggc gataattcaa aacctaccaa tctgtcaaga
    31381 attcgtcttg cttgttgtgc gtcaacaagg tgaatatcta ttttacgtgg attctgaata
    31441 gcttccaaga ttgcatcttt ggtgatttcg tgaaaaacaa ttctttttgt tgccgaatct
    31501 ttcagattta acacttcgga tagatgccat gaaatagctt ctccttcacg gtcttcatcc
    31561 gttgctaacc aaactgtctt tgccttacga gataattttg tgagctcgtc aattactttt
    31621 gtcttatcct cactgacctc gtatatgggt tcgaaattat tcttgatatc aatgccatta
    31681 ttggtttttg caaggtcgcg tacatgacca tagcttgact taacaacaaa gtcttttcca
    31741 aggaatcctt ctattgtttt tgcttttgcc ggtgactcaa caatgactaa attttctgcc
    31801 atctgctact ttttgaaggg cgcaaattaa atcatttcat tttgttattt ccaaacgaaa
    31861 ctatggccaa tcatttgccg gcttgtttat tttaaagtac ctttgcaacg ttttacagag
    31921 ctaaaaccat aaaacctgat cgggttaaaa attgttgaaa acctctttaa aacttttaaa
    31981 acaaaaattt tactaagaaa atgcataacg acataaaagt aattgatgaa agccggcagg
    32041 gtgaacccac catgctgcaa tcatcatcag aaaatctgag tcctaaaaaa ctttatttag
    32101 aaagttatgg ctgtcagatg aattttgcag acagtgaaat tgttgcatca attttgaaga
    32161 atgaaggttt tgaaaccaca aagaatgtta atgaagccga tgcaattttt gttaatacct
    32221 gtgccattcg tgacaatgca gaacaacgtg ttcgaacaag acttaaagat tttaagaaga
    32281 taaaaaaaga aaatcctgat ttgattgttg gcgttcttgg ctgtatggca gaaagaatta
    32341 aatcgcaatt tctggaagaa gaaaaactgg tggatattgt agttggccct gatgcctatc
    32401 gtgacttacc actgttgatt gaaaaagttg aagacggaca aaaggctgtc aatgtaattt
    32461 tatcgaaaga agaaacttat gcagacataa ctcctgttag attaggcaac aacggtgtaa
    32521 ctgcatttgt gagtattatg cgcggttgtg acaatatgtg ttctttttgt gttgtaccat
    32581 ttacacgtgg tcgtgaacgc tcacgcgacc cacagagtat tattaaagaa gttgaacaac
    32641 tatttaacga aggctacaga gaagtaacat tattaggtca gaatgttgac tcctatttat
    32701 ggaatggtgg cggactgaaa aaagatttag atactgaaac tgttcatctg gcattgaacc
    32761 ataaactacc atcagaacag caaagagatt ttgtcacttt ttcaaatctt cttgaaatgg
    32821 tagcagaagt gaatcctgcc atgcgtgtcc gattttcaac ttcaaatcca cgcgatatga
    32881 ctgatgcagt attgtatagc atggctaagt acgaaaacat ctgtaagtat atacatctgc
    32941 ctgttcagtc aggcaacagc agagttcttg aaaaaatgaa cagaggttat tcacgcgaag
    33001 attacattac aagaatagaa agtataaaaa gaattttgcc tgactgtggt atcagcacag
    33061 atattattgc cggcttttgc agtgagacag aagaagaaca tcaggatacc atcagcctga
    33121 tggaacagac tatatatgat tttgcattta tgtttgttta tagtgaaaga cccggaacct
    33181 ttgcagaaag aaaatttaca gacaatgttt tgcctgaagt taaaacacgc agactaaacg
    33241 aaatagttgc attgcaacag aaacattcag caatcagaac caatcagtca ataggcaaaa
    33301 cgtttaaagt tttgattgaa ggtgtttcaa aaaaatctga tgagatgctt atgggaagaa
    33361 attcgcaaaa cactgtggtt gtttttccaa aaaataatca taaacccgga gagtatgtta
    33421 atgttaaagt aaataagtgt acgtcaacta cattgatagg tgaagttgta tgataaattt
    33481 tgtaaaatca aaacaactgt tcccgttcgc tgttaaagaa aaacacaaaa agaaacacaa
    33541 agttaacaac cggcaatgac catacaagac attaaagccc gatttggaat tattggtttt
    33601 tcatcagtac ttgaccatgc attaaacaca gcaaggcagg tagctccaac agatttaacc
    33661 gtgttgatta caggtgaaag cggttctggg aaagaagtgt ttccacaaat catacatcag
    33721 ttaagttcca gaaaacacgg cccttatatt gctgtaaact gtggtgcaat gcccgaaggt
    33781 actattgaca gcgaattatt tggtcatgag aaaggagcat ttaccggtgc gcatgaaatg
    33841 cgaaaaggat attttgaagt atcgaatggc ggaacaattt ttttggatga ggttgcagaa
    33901 ttacccatgt ttacgcaggt tcgtttatta cgtgtacttg agtcaggtga gtttttaaag
    33961 gttggttcaa gtaaggttca gaaaactgat gtaagagttg ttgcagctac caatgtaaat
    34021 attcctgaag caatcagcag aggacgtttc agagaagatt tgtattacag attaaatacc
    34081 gttcccattc atgtacctgc actgcgcgag agaacagaag acgtgcattt attattcaga
    34141 aaatttgcag gagattttgc agaaaaatat cgcatgcctg cacttacact tcagcccgaa
    34201 gctttgcgtt tgctgatgga atacagatgg ccgggaaacg tcaggcagtt gaagaatgtt
    34261 acagaacagc ttagcatcat tgaacagaaa aaagaaatca gtgcagaggt actattaaaa
    34321 tatttgcctc aggaaaattt tgcaagcgta cccaccctat ttaataacaa caaaggtggc
    34381 gatttaaatg agcgcgaatt gttatataaa gttttgtttg aaatgaaaaa agacatttca
    34441 gaacttaaaa agatagtttt taatattatc agtaatggcg agcctgtaca tcttgaaaat
    34501 tcaggtattg atacagaacg atttttaaat gatgtgcaag ggcatcctgt attgcacaca
    34561 aacgatactg caattacact tacaaaaccc acacatatag atgtaaaacc acatgagcat
    34621 attgaagaat cgctgagcat tgccgacaga gaaaaagatc tgattatacg ggcattggaa
    34681 aagtacagag gtaaaagaaa aggtgcagca aaagaattag gaatttcgga gcgaacgcta
    34741 tatagaaaaa ttaacgaata cggcattaaa gactgatgag taacataata atgatttcag
    34801 gtttattaaa gcgtaaatta ttctcaggat taattgttct gattgcattt actgtaatga
    34861 tggaatcgtg cggtgtttat tcttttaccg gtgcatctat tacgcccgga gtaaaaacag
    34921 taagtattca gtattttcct aacagagcat cggtagcgca gccatcatta agtcaggttt
    34981 ttactgataa actcaaagaa aaatttgttt cacaaacttc gctcattcag gttaatcaat
    35041 cgggcgattt acgttttgaa ggatatatta gtgattacac tgttcagcct actgcaatac
    35101 aaagtacaga tgtggctgca ctaaaccgcc tcaccattac cgttcatgtt aaatttgaaa
    35161 acatgaaaga tgaaaaacag aattttgaaa ctagtttttc gagatatgcc gactttgatt
    35221 ccaggaaaga tctgaccagt gttgaatcat ctcttatgaa tgatatatct tcgcagcttg
    35281 tagatgatat ttttaataaa tctgttgtta actggtaatg actaaggaac gtttttttaa
    35341 tttcattcaa aatccgttaa gcgttcatgc caatgacatc gaagagttgg aacaaatcat
    35401 tgagcagttc ccctattttc agactgccag gttgatttat accaaatcac tgcacaatga
    35461 aaacagttat ttgtacaatg atgaattaaa gcgaactgct gcctttgctg ccgacaggag
    35521 tgttctttat aaactcattc attcaattaa aaaagacgtt acaattgatg agccggaaag
    35581 tttgattaca gaagatatag gatcaaaatc ggtaattaat cctgttcaag aagtacctga
    35641 tttgaatgtt tttgtatttg aagaaaaaac agaaattgaa gagcaggcaa ttgtatttga
    35701 agaaaaagaa aattctgaat cagcagatga tgaactcata atatttgaag aaaagacatt
    35761 aacaattgag gagggcgagc cgattgtttt tgaagaaaag cgaccagaaa ttaccacaca
    35821 ggaagatata gtttttacag cagttgacga acccatcaat gccagtgcag aagatgatga
    35881 agttgggtct tcaaataaaa ttgcagaaat agagtcagag aaaaaagagc gtgtgctgac
    35941 acctgcagaa attttatctc aaagactacg agaaatcgaa tctcaattag aagacaaaaa
    36001 agaagatgaa actgaagtag tctctgaatt gaaagaagaa acaattattc ctgaaacacc
    36061 tcaaattgca gaacctttaa atattcaatc tgaagttttg ccggaaagca ttcaaattag
    36121 ttcttctata ccggaaatta aggaagaagt agcagagatt tctgaacaag cagaagcaga
    36181 aaaactaata cccgctgaac ccgaaagttc agaaaaaaca attgaagttg ttgcaataag
    36241 tcctcaaatt caaccacccg taattgaaga agttgttgca cctgatgcaa tagttcaagc
    36301 tgaaataaaa ccattgccag aggctgataa aaaagaaaaa cattcgttta cagactggct
    36361 gcatcgtttt catccacagg aggaaaaaaa aactttaagc cctgataatt tagaagagga
    36421 taactataac aacaaactga ttaacagtca attaacgtca tacattgaaa ctaaaaattc
    36481 aaataatgaa atagttaatc agtttatcca aaacgaaccc aggattgaca gtagcaaaac
    36541 taagtttttc agtccgggca atatggccaa gtcaagtgtt actgatgtat ctgacatagt
    36601 ttcagaaacg ctggcaaaaa tatatttagg gcaaaacaat ttcagcaagg ctattcaaac
    36661 atacgagaag ctgatgttga aatatccgga aaaaagtgtt tactttgcag cccttattaa
    36721 agaaataaaa aaatcacaga tttaacattc aatctatatg tacactttaa ttaccgtgtt
    36781 aattatttta attagtgttt tactttgcct tgccgtattg gtacaaaact caaaaggtgg
    36841 cggtttagct tccggatttt ctgcaccaca gcaaattatg ggcgttcgta aaacaacaga
    36901 ctttcttgag aaatttactt ggggattagg aactgcttta atcgttttct gcgttgtagc
    36961 aaattttatg actccaggca gtaataacag tactgaagta agagcttctt ctatacaaga
    37021 gcaaattgac aatgcaacgc ttcctgctgc accacaggca ccattgccat cgcagcaaaa
    37081 tgcacaacct gcacagcaac ctgctgcaca gcctgcaaat taatcttgtc agttaataac
    37141 ttttaaaggt gccataatgc tgaaattatg acacctttat tatttaagcc aaagccataa
    37201 atcgcaaaac tgtcagtaaa atagtcatgg cacagaaact gataaaggca aatagaaatt
    37261 aattataaag tataaaagtc aaacatttta aatcacatta ttactatgag taaaattagt
    37321 ttaaaaccat tggcagaccg cgttattgta gaagtggctg cagcagaaga aaaaacagca
    37381 agtggaatta ttattcctga cacagcaaaa gaaaaacctc aaaaaggcaa agtagtagct
    37441 gttggtggtg gcaaaaaaga tgagccaatg actgtaaaag ttggtgacag cgtattgtac
    37501 ggaaaatatg caggaacaga aatttctgtt gaaggcaaag agtaccttat catgcgtgaa
    37561 tcagacatct tcgcaattgt ttaaaagctt tagaaaacaa aaagaaataa ataaacaact
    37621 taaataaaat acgaaaaatg gcaaaagaaa tcacatttaa ccttgatgca cgcgatgctc
    37681 ttaaaaaagg tgttgatgca ttggcaaatg cagtaaaagt aactttagga cctaaaggcc
    37741 gcaacgttat tattgataaa aaattcggat caccaacaat tacaaaagat ggtgttaccg
    37801 ttgcaaaaga aatagaactc acgaacagta ttgagaacat gggtgctcag atgctgaaag
    37861 aagtagcgtc aaaaactgct gacattgcag gtgatggtac aacaacagct acagtacttg
    37921 ctcagtcaat tgtaaacaca ggtttaaaga acgttactgc cggtgccaat ccaatggatt
    37981 taaaacgcgg tattgacaaa gctgttgaag ctgtagttgc atcattaaag aaaatgagca
    38041 aagaagttgg tgacgacaat aagaaaattg agcaggtggc tacaatttct gcaaacaacg
    38101 atagcgaaat aggaaagctt attgctgaag caatggctaa agtaaaaaga gaaggtgtta
    38161 ttactgttga agaagcaaaa ggtactgaaa ctacagttga aattgtagaa ggtatgcagt
    38221 ttgacagagg atatatctct ccatactttg ttaccaatgc agataaaatg caggctgttt
    38281 tagaaagccc atacatttta ttgtacgaca aaaaagtatc taacatgaaa gaattacttc
    38341 caatattaga gaaagtagtt cagacaggaa agcctttgtt gattattgct gaagatgtag
    38401 atggcgaagc attagctaca ttggtagtaa acaaaatccg tggctcactg aaaattgcag
    38461 cagtaaaatc tcctggcttt ggcgatcgca gaaaagcaat gatggaagat attgcaatcc
    38521 ttacaggcgg cacattaatc gctgaagagc aaggattcaa attagaaaat gctgacctct
    38581 cctacttagg cacatgcgaa aaaatcacta ttgataaaga caacacaaca attgttggtg
    38641 gtgcaggaaa aaaagctgac atcactgctc gtgttaatca gattaaagca cagatagaaa
    38701 ctacaacatc tgactacgac aaagaaaaac ttcaggagcg tttggcaaaa ttagcaggtg
    38761 gtgttgctgt actttatgtt ggtgcagcta cagaagtaga aatgaaagaa aagaaagacc
    38821 gtgttgacga tgcattacat gctacacgtg cagcagttga agaaggtatt gttgcaggtg
    38881 gtggtgttgc atacattcgt gcaataacag ctttagacaa acttaaaggt gataacgatg
    38941 atgaaacaac aggaattgca atcatcaaac gtgcacttga agagccatta cgtcagattg
    39001 tgagcaatgc cggaattgaa ggaagcattg tagtacaaaa agtaaaagaa ggcaaagacg
    39061 actttggtta caatgcccgc tcagacaagt atgaaaacat gcatgctgcc ggagttattg
    39121 atcctactaa agtaacacgt gttgctcttg aaaatgcagc ttctgttgct gggatgttac
    39181 tcactacaga gtgtgtactt gccgacatta aagaagataa gcctgcaatg cctgcaatgc
    39241 cgggtggaat gggcggaatg gattattaat catctgcaat ttcaaagtac ggaaaacgta
    39301 tttcggaaca ataaacaaaa ccctctgttg gattttacct tcagagggtt ttttatttgt
    39361 cagttgtaag gtttagaaag ttggaatgtt tggaaaattg gctttactct ataatcaaac
    39421 atataataat tctactgtcc tcactaagtt cctatcttac ctactccgct attttgctat
    39481 aatccaaaat aaaaaagtcc cgacattttt ttgccgggac ttttttataa ataatttaaa
    39541 actttcttta ttcagtagat actttcagga ttttctttcc ttcaaacaca taaatgcttt
    39601 ttccatctaa agaaacaata gattttgaat ttttgcgtga gtcgaagaat tttgctttac
    39661 cggaattggc atcaatagaa aaataatcgc ctttctctgt atcaaagaaa atattttctc
    39721 cgaaaattcc tgaaaactct ccttttttgg tttttactgc ccacactttt gcaccagtag
    39781 caatgttatg agaagaaacg cctttctctc ccaaaacaat tacattgtct ttactaatat
    39841 tatcacctaa ttcagttgga tttacaattt tttctgccaa gccaatatca tcatcaccta
    39901 aaggaatttc gtaggcttct ttgcctgttt tgtaatctaa agagtataat gccttaccac
    39961 ttgacactac aatgttattg ccgcttggga atatatttgt aattcctttc ctaaagcgct
    40021 cagagtccca agattttgta ccatctgaag tactaaacgc ctgcacttca taaggcttta
    40081 cattctgata ttttacagtt ctgtaatatt gtgtccattg tacatagccg ttaccataag
    40141 tttcttttga tatagcctgc acttcaacag cgcctccaat ttgcacaatt attctatcgt
    40201 caattttata caagcccgga ataactcttg catctttaat ttcaggagaa gaccaaacaa
    40261 gtttacctga gttgatttcg tatttcttta aatactgact ttttctgctc tttacatcta
    40321 aaatatatac gtactctccg tcatacaatg gttgtgcaat ggcatcataa acaccatatt
    40381 ttacaacctg gccatatact ttgggcctca ctaattctct tgcaaaagtt acatcgtaag
    40441 ctgctgccca aattggactt cctgttttat attcataaac ctgcaagcca tttaattgta
    40501 aaaacaactt atcgtctctt aaaaatattt tagcaacaga ttcgcgggta acgacttttc
    40561 tttctactat tccaacaaag gtttgctccc ataaaatatc accattctta gcgttgattt
    40621 tcagaatctg atttctaaaa cctttaaaca tggcctgcca tgcatttctg ggcatgctaa
    40681 tgatgacaat tgcattttct ttttcatcat aaaagtattt accaaccggt gtactaaatt
    40741 taactgttga ccaaagttcc tctccagttc tggcttttac catggttaaa tttgttttgg
    40801 ttacaatagc ataagcattt aattctgcaa tgtaaataac ctcatccgga tcgtcaatgc
    40861 cttgatattt attggtactc caaagtttag tacctgtttc catgtcaacc accaccattt
    40921 ggtctttacc tgctttttta tcaaataaaa acactgcatt cgattcgtac attggaattt
    40981 gtaaatctac tttcccaatt tgctcatcaa tttctttaaa ctttttactc cattttactt
    41041 taccgtcaac agcggaaacc atggtaaaca ttttttcatt gctaccatgt acgatttctt
    41101 tcttttcgct caatccggtt tctgtaaacg gatgtgtggt ttctatctcc cacgtggttt
    41161 tcatttcatc gtatttctga gcgtagctca tcatacaggt cagacctatt gtcagaccgg
    41221 ttaaaattag atttttcatt ttttattgtt tgttttaatt aattaaaatt tatctttttg
    41281 ttatatgtta ttttcaatac taaaggtgta tctgaatttc agtcgttgat ttggagaaac
    41341 aacaatgtct ttaaatttat atttgagtaa caaatctttt aaagcatttt gatgagggat
    41401 gtcagtttta tcatccgatt cagcaaagat ggtaactatc tgtccttttt tctgaacagt
    41461 taaatcaaat aagtatatac cttttatgtt ctgctttact atattttttg acaaatcact
    41521 ctccggattt tctaagtcgg cattaagctg actaattgct tttgattgta aggagtcgtt
    41581 atcttgtcct tcaatataat cagtatattc ttttaaggtt tcataattcc attctatcag
    41641 ctttgtgcca ttggataagt aggctacagg ttttgtttta ttaaaggaaa aataaatagg
    41701 aaaaatattt gagcttcctc tcctgccttg gtaacggtat tgcccaactt tgtttttatg
    41761 atcgttatta tcaattaata cgagtttacg cttccatcca ataaatccgt cattgtctga
    41821 atatccgaaa taattagagt aagtactaaa cttgtaatcg gcatcaggtt cggcagttgc
    41881 atgataaatt tctttaacta agttaccatt gcttgcatct aaaacctgaa tattagtttg
    41941 tcttctatta tcggcagcag ccattgatat gccatttaaa cctgctgtac caatgcgttc
    42001 agctactacg ttatgcgaga gtaaataagt ttgcttatca tctctgctga atttaataga
    42061 ataaacaatg tctatttctt cttttgagtt ataaagtttc tttaaatcgg ggtagctgta
    42121 aaagctgatt agtcgcttgt tctttctggc cgtttttaac tcacttttat ttttaccaat
    42181 agattctact tctttaaagt cagctttatc gggatcaaaa gaaagtgcaa ttaatttatt
    42241 gtcgtggctg atggctgcca tttcgcaatt atctacttct aattgagcta ttactttgtt
    42301 tgataaaaag tctttcacca taatgccatc atttgatgct attaaaattt gtgtgctgtt
    42361 gcttaaaaac tgtactgagt ttacattgtt tccttcagaa acaacttttc cgtcagcggc
    42421 atttaaaata taatatttta tatcttttac ctttcttgag gtaattgcag tttttaaagc
    42481 tgacgattcc tgcaataata tatattggtc gttctcgcta aattgaggtt taccaccatt
    42541 gtttagcccg gaaaggttta aatttttcca aacagggttt acctctgtgt tggcttcaac
    42601 atcgcttacg ttaataaaaa agatacgtcc ttgcgaggcg gttaaaacta ttttatcaga
    42661 ggcatgatta gtttcaatgc ctaatatggg atagttgcaa tcgtctattg ttatagttcg
    42721 ggttccggca ggttgggcat aaacagttgt tacaaaagca agactgagga tagctaatag
    42781 aatgggcttc ataagttaat tgtaaagttc tttttcatat ttgtattgta tgcagttgac
    42841 accactttca atgatatgtt aatacgataa acagaaatta atgttacaaa gtgtttttga
    42901 atgccatctg tcgtgagata ctttctaaag aaaaaagaac ctcgcaaaga ctgcaaagaa
    42961 tgtaacacgc aaagactgca aagaatgtaa cacgcaaaga ctgcaaagaa tgtaacacgc
    43021 agagaccact aagaatgtaa cacgcaaaga ccgcaaagaa tataacacgc aaagagcgca
    43081 aagaaaaagt tgaactcatg gtattctatg taaatcttat tttatagttc tactaaaagg
    43141 atagcctttc tgaactaaaa aatattttca aaaacaattc aacaactttc aaactttgca
    43201 actttctaaa ctttccaaac tcaaaggctt aacgataact attcctgaac ataattttaa
    43261 taaacgttct ttcaagtatg gcctgtgcta gtgcttgctg attatcctgt ttatttaaaa
    43321 ctttctttaa tgttttctca ggaatgtcaa ctctataaag ccatgcattt aaagttgaag
    43381 agcctgattt tgccaactga cggataatta cttctaattg cattataata tcctgataag
    43441 ctgaaaaagt atcgccactc catgcaacag taaatccaca aggaattaaa tctttgttta
    43501 attgcacaac tgtttgttca attaagttac gttgctgaaa attctgatta gaaagttgtt
    43561 ccatttccat cctgctcttc ttaagtcagg agtttagcaa tgcaagcagt gcaagacggt
    43621 aggaatttaa tccaaatcct gtgattacac ctttacaatt ggcagcaagc agacttttat
    43681 gtctgtactc ttcgcgtgaa tgaatattgg aaatatgcac ttcaaccacc ggtgacggta
    43741 ttgccgcaat cgcatctgct atggcaacac ttgtatgtgt ataacctcct gcattgatga
    43801 tgataccatc ggctttaaat ccttcgttat gcaaaaggtt aataatttca ccttcaacat
    43861 tactctgaaa tatttgaata ttgggttcag gaaattcttt ttttaatgtt attgcaaaat
    43921 catcaaatgt ctgatgacca taaatttcgg gctcacgttt gccaagaaga ttcaggttag
    43981 ggccgttgat gattacaatt ctttttgcca taaaaaatta tgggatgttt aggtacttat
    44041 gcgtttgtag tgaaattgac cactgaggat ttctctttat gtaatcaatc attatcggaa
    44101 taactctatc aaacctactg tattcaggtt gtagaaaaag ttttgcaaca tcagaggctt
    44161 tacgtgcatt ttcttcagcc catttaaaat ccgactcatt aaaaataaca accttcagtt
    44221 catttgcttt ttgtaaaaca gattccaacg gtgctttaaa ttttttaggc gaaacacaaa
    44281 tccagtcaaa ggtaccctga attggataag cacctgatgt ttcaagccac actttatatc
    44341 ctttcttgga tagtgcatcg gtaagtttgg ttagattata tgctgccggc tcgcctcctg
    44401 taataacaac attaggcgtt ttactttctg ccacagcttt caacagatta tcaacagaaa
    44461 tcagatttga tgttgaagcc tcccatgatt ctttaacatc acaccaaaca caacccacat
    44521 cacagcctgc aagtctgata aaaaaagcag gtttaccggt atgatagcct tcgccctgaa
    44581 tggtgtaaaa tttttccatt aaaggataat atccttccgg cagattggcg agaatattct
    44641 ttttgtaatt ttcagtcacg gcaggttgca ttgaaaatca tcaatgattg atctgattta
    44701 ctacacgctg taaaatttcg aaagtatttt cagccatcag attttcttta tcaagtgttg
    44761 gattgacttc gcaaatttcg aaacaacaca ctttttcatt ttgcaccaac cttacacaca
    44821 gttttcctgc atcacgctct gaaattccat ttcgtacagg agtacccgtg ccttttgaga
    44881 tagagctatc catgctatca acatcaaatg aaacatagat gtgagtgcaa tgtgagagat
    44941 gtgccaatgc agtacggaca attttctcta ctgctatacg ctttacttca gcagcagaaa
    45001 tcatttttac attatgtttt ttcaaaagat atgcctcctg aggttcaata tctcttacag
    45061 caatatagac tatatcttta taggtaatct taggcgacat ccctcctatc ttcttcaact
    45121 ttgtccaaag gtcaacagtt tctttatcgg gtttgttgat tttgtttgca agattgtcct
    45181 caccaagatt tgctgccata ggcataccat gaacatttcc tgttggggta gtatatggag
    45241 agtgaatgtc ggcatgggca tcaatccaga ttacacccag cttcgacttt ggataagtca
    45301 ttttaatgcc tgcaacggta ccaccggcag tgctatggtc tccggctaac acaatcggga
    45361 aatctttatt ttttaatgtt tcagaaacgg cctttgccac acgttcataa atagcaacaa
    45421 tgccttttat acgttttgca taaggagtac ccgctgtctc gaagagtaat tggttttcag
    45481 tcttgatttc tacactttct atcctgttga aaagtgcact gccatagtct aaggctgcaa
    45541 tttttattgc atcaacacca aggcttgcac ctctggtacc ggcaccaatt tctgatttaa
    45601 cttcaatgaa ttttatttta cttgccataa attttttaac atcgttattt tttattcagg
    45661 ttatttttac attcgcaagg cacaaaaata agcctttcgt attgcaaatg aagaaccgtt
    45721 acattgacct gattcaacaa agttacgact ttccacaaga aggttttgaa gttattgacg
    45781 attcactcca ttttaatggt ataaacctga tggatgtgat aaaacaatat ggcacaccgt
    45841 tgaaaataac ctatctgcct aagattagct cacaaattca aaaagccaaa cgacttttta
    45901 aagttgctat ggcaaaagtt gactataaag gaagttatta ctactgttat tgcacaaaaa
    45961 gttcgcactt tcagttcgtg atggaggaag cactgaaaaa cgatattcat attgaaacaa
    46021 gttcggcttt tgatattcct attatcaggt cgctctatga gtcgcagaaa ataaacaagg
    46081 acatttatat tctttgcaat ggttttaaac gcgaaggtta tattgaaggt attgttaatc
    46141 ttattaaaga tggttttacc aatgtgattc caattctgga taatgttgat gaacttcagg
    46201 aatacgagaa ggcaatgccc ggcaagaagt ttaaaatcgg aatcagaatt gcttctgaag
    46261 aagagcctaa tgcagagttt tacacttcac gtttaggtat caggcaacgt gacattgttg
    46321 acttttacaa gaataccatc aagaagaacc ctaatgttga actaaagctt cttcattttt
    46381 ttatcaatac aggcattcgt gatactgcat attactggaa tgaacttcag cgaaacattg
    46441 cattctatgc agaccttaaa aagatttgtc ctgagttgac agctcttgat attggtggtg
    46501 ggtttccaat taaaacgaaa cttgaattta atttcgacta tcaatatatg gctgacgaga
    46561 ttgtaatgca gataaaatcg gcatgcgatc agcgcaaagt gccggtacca catttattta
    46621 ctgagtttgg cagttttact gttggtgaaa gtggtgcaat tttatattct gtcacaggca
    46681 ttaaacaaca aaacgacaaa gagttgtggt atatgattga cagtagtttt atcactacac
    46741 tacctgacac atggggtatc aaacaaaaat ttattctgct tgccatcaac cactgggata
    46801 aggaacaaca acgtgtaaac ttaggtggca taacttgtga tggtgaagat tattatatta
    46861 tggagtcggt aaaaaatcgt gtttttatgc cacgacctac acctgacgaa ccattatatc
    46921 ttggcttctt tcacaccggt gcctatcagg attcactcgg tggatatggc ggtattcagc
    46981 attgcctatt gcctgcacca aaacatgttt taatagataa aactgaggag ggtgaattga
    47041 gcatgtggct ttttgcaaaa gagcaaagct caaaatctat gatgaaaata ttagggtatt
    47101 aatttccgaa attcaaaaaa atcaatttcg tttattaaaa ttaactcagc ctaactattt
    47161 cgtaaactcc tcaaacgttt ttgttttgta ggcatcatgt gcatagtact tcaacaaagg
    47221 cattaaagtt tctacaggca tatagccttg ctgtactttt atagcagcaa atttttcgtc
    47281 aagaaatgca tacgatggat aacccatttg acgattaagc aatgaaattg ccagctcatg
    47341 acttttatat tcaggaacaa aaacaaatac ctgatcaaaa aagtgtatag tatccttact
    47401 ttcagcatta aacttaaccg gataatagtt tgcatttata aatgctgcaa cggagtcatc
    47461 catgaatgtt gatgcatcca tttttttaca ccatccgcac cagtctgtat aaacatcaat
    47521 aaataacttt ttaggatttt tttgagaggc agcaacggca gactcaaagt ccatccattt
    47581 tattttatgt tcaatttttg aaggttgcga gcaagaaaca tatcctgtca ggataataaa
    47641 actaaataca acacctatta aattcctttt catctttaat ggatttatac taattattca
    47701 acgtcttttt ttcgggttta attgcaatgc ggtaaagtat tacagacata aaggtaaaga
    47761 atcctactcc taataactga taaccgtctt caccgattac agcagcaata ctatgttcca
    47821 tattaacttt ttcaagagcg gcatacacgc cttcatcaac agaaagcaat gctactacca
    47881 cgcctgccaa tgcaccaccg gcaaccaaac ctgtagcaaa caaatttcct ttgcctaact
    47941 catcatcacc ttcggccttt ttatgtttac gttttgcatt ccaatctaca atacctttta
    48001 ccataccacc tgcaaaaatc ggcaaggttg tactcaaagg taaataagct ccaacggcaa
    48061 acgacaaagc tttaacacca cacaactcca tagttattgc aataaatacg ccaacaagca
    48121 caaattgcca gtcgagatta taactcaaaa gtcctttgat taatgttgcc atgagtgttg
    48181 cctgcggtgc tgcatatttt tcaccaatgg catgatgaat accttgtgca gtcaggtcgg
    48241 cagtaggcgt gtcaagatat tttacagtga aacctataac tactgacgaa acaatagcac
    48301 caatgaataa agccatttgc tgataacgtg gtgttgctcc aacaatataa cctgttttta
    48361 aatcttgtga agtggcaccg gcatttgcag ctgcaataca aaccaaacca cctacaacaa
    48421 gtgccatagg ttcaaaaact tttccagtcc atcctattgc gataaacact aatgctgttc
    48481 ccatgatggt tgcaatggtc atccctgaaa ttgggttatt actgctaccg atgataccaa
    48541 caatgcgact tgaaaccgtt acaaagaaaa atccaaacac tacgattagt aatccaatca
    48601 ataatttatt caggaacgat tcaccgggca cctgaggtaa taatgccatc agaataatta
    48661 aaactacaga acctacaatt actgttttga atgataaatc attttcagta cgtgaaactt
    48721 tctgctcttt atctccactt ttcagcgagc ttacgctttc tttaaaagaa gaaataattg
    48781 ttggtattgt tttcaatagt gtgataaatc ctcctgctgc cacagcacct gcaccaattt
    48841 gtcttacata agcacgataa attgcatcgg ccatatcagt aaatgtatgt gtctcaggat
    48901 tccatcctcc gggtccactg gcagttaaaa catcttttag gtagcctaat ttcacaagtt
    48961 gtgctgcaat catatcagtg ggcactaatg ttgctaacaa aggaattaat ccaagccatg
    49021 ctagcacacc accggcaaca agcacccctc ctatttttgg accaataata tatccaacac
    49081 caagatattc gggagtaatt tctccactaa cagtagccga tggcagatat ttgttggttt
    49141 gtttagttac aaatgcaggt acttctgcaa tgacatgaaa tactttttga aaaatggcat
    49201 aaagcatggc aaagcctaaa ccaagatagg ctgtgcgtgc aaaatcacca cctttttcgc
    49261 ctgctataag aacctgagca caggcagttc cttcgggata aggtaatgtt tcatgctctt
    49321 taacgataag acttctgcgt agaggaatca tcattaatgt acccaacatt cctcccagaa
    49381 cagctaatgt aaagattgtc caatattcaa aatactgaat cccgttactg atgccacttt
    49441 catttacaga taaaaataaa aatgcaggca aagtaaaaac cacaccggag gcaatagact
    49501 ctcctgcaga gcctgttgtc tgaataatat tattctcaag aatagtagtt ttaaaaaact
    49561 tgcggccaat acttattgca agaacagcaa tgggaatcga agcagagaca gtcaaccctg
    49621 cttttaatgc cagataaact gttgatgccc cgaagatgat tccaaacatg gagccaagca
    49681 gaattgattt gagtgtaaac tctgccattg aatctgttgc tgagatgtat ggcttaaatt
    49741 ctttttgtgg ttgcataaat aaaaagtgag atggctaatg taaaggatat tttaattagc
    49801 caaaacaaaa aaatgttaac gcttttactg attggtatga aaaccttgtt tttgcaaaat
    49861 tactttaact ttaatggcaa accaggctaa gtacgcaaag caaaagaccg ttatccaata
    49921 gctggaatga atgtctgtta catcggcaag ataaccctgc aatggaggaa ttaatgctcc
    49981 tccaagtatc atcataatca gaaaagccga gccttgtccg gtaaactttc ctaatcctgt
    50041 tatacttaaa gcaaaaatac aaggccatcc gatactgcaa cacaagccac cactgatgaa
    50101 ggcaaacatg gctacctgcc cttcggtaaa aagacctatt aacattgcca aaatacctcc
    50161 tgcagaaaaa ataaacaaca tgcgcgcagg cttttcctga gctaaaaaac ttccggctat
    50221 gagtgctgca acacaaagac tgtatggcaa cagatttgta atttcattac cacgaagata
    50281 gttggctaat aaaacaatga tatatgcaac tattggcaac acaatcataa gcagtttttt
    50341 ggttgaaggc ttaaaattaa aagcactgat ggcacctgtc caacgaccaa tcattaaact
    50401 tccccaataa agagaaatgt aaggagatat ttgagctgtt gtgagtcctc cgaactgcgg
    50461 caatgccaga agggcaccca tgttgctttg aatggttacc tctacgccaa cataaacaaa
    50521 gatagcaagc atgccccatc gcagttgcga aaattttaaa gcacctaaac ctggttcaaa
    50581 cttttctgtg ctgtgtacct ttgggagttt agcaacagca agcaataacg ctgcaagtaa
    50641 aaaaactcct gtcagaatat aataaactgt atcaacagat tctacagttg cactggcagc
    50701 agcttcgggg ctaaggtttc caaacaaaac aaggctgact aatattggtc caagcaatgt
    50761 tccaaaggaa tttattccac caccaaggtt tagtctatgt gcacctgttt cggcagtacc
    50821 taaggctgta acaaaaggtt gtgcagcggt ttgctgaagt gagaacccta atgcgatgac
    50881 aaaaaatgca gaaagaataa aaacaaaaga tccggaacct actgctggaa tcatcaacaa
    50941 agaacctgca acagaaatca ataatcctgc aatgacggct tgtttatatc ctaacttatt
    51001 gaggatatca acccggaata attgtgagct gaaccacaat aataatgagc caataaaata
    51061 agctgcatag aatgctgtat caattaactg agattgaaat tgtgataaat taaagtgcga
    51121 cttacaaaat ggtatgaata ttccatttga cgctgccaca aaaccccaaa agaaaaatac
    51181 tgtaatgatt gttgctaatg cacctgcatt ggaagatgat ttcatttgat gatgattgat
    51241 attccaaaca tcagcataaa acattattgc aatttataac aacaaggaag ttttccgcta
    51301 aagcatgtta acaaatggtt tactcctctt cattgtcata taattcaatg agaggctcgc
    51361 taacaatttc tttcctgctc acccacttgt caattgaaag caacaaagaa ggcaataaga
    51421 ttaaattggt aatcatggcc acaagcaagg ttattgaaat aagcagtcct aaagctgcag
    51481 ttccaccaaa atcggaggca gcaaaaatgc caaatccaaa aaacagaatc accgcagtat
    51541 aaatcattga gaaccctgtg tccattattg ttgcagatat tgcttcacct attgactttt
    51601 tatgttgctt taattcctgc ctgtatttag aaagaaaata aattgtacca tcactggaaa
    51661 taccaaatgc aatactgaaa atcaaaattg ttgatggctt aaaacgaata tcaagaaagc
    51721 ccattattcc tgcggtaatt actaatggaa tcaaacaagg tagtttgctt aaaaggataa
    51781 taccgattga acgaaacaat gccattccga caatggcaat cagtattatt tcaataatca
    51841 ggctttcata tagattgtat aataaatagt cattcccttt taaaaacatc aggctatgcc
    51901 ctgtaagttt tacctgatat tgtgaactat cgaaaattga atctacctta ggttgaatat
    51961 cagctaacag tgttttaatt ttctttgagc ctacatcagc catctgcata cttatgcgtg
    52021 tatattgtct tgatgaatca ataaaagatt tcaatctgtt ttcatttcct tttactgtag
    52081 aggtataatc attcagattt ttaagttcac tgatgcctgg taatacataa tattttggat
    52141 caccacctcg ataagcctga tagctgaatt ttaatgcttc ggttattgac aatgctttac
    52201 ttaattcagg atacttttcc aattgctttt gtaatgcttt cattttgtat agcacacgtc
    52261 cgttattatc aaaaacacca ttcggttttt ttgtgtcaac aaaaatttca aatggcaata
    52321 cacctttaaa atttttttca aagaagtgca tatcggtgta aacaggattc ttttttggta
    52381 aatcatcaac cacatatcca gtgacatcaa tttttgtcat accgtaaatt gctagaaggg
    52441 taactgtaac aattacggta taaatctgat ttcttttatg atgaacaata aaatcaaccc
    52501 attcaagaat cttacgcatc cttttccctt caagatgacg catctgtcta acagaaggtg
    52561 gtggaagaaa actaagaata atcggaataa aaattaaggt gatgaaataa gttaccatta
    52621 cattgatagc tgcaatagca ccaaactgag taaggaatga actgttagta aataccaaca
    52681 caccaaaacc aatggctgtg gtaatgttgg ctaagaaaag tgagattccc actgtagaaa
    52741 tcattcgcgc taaggcttta atacgattac catgccttgc atattcacta tgatatttat
    52801 tgataagaaa aatacaatta gggacaccaa taaccattat aagtggtgga atcaatgcag
    52861 ttagaatggt gattttgaaa ttaaataatt caatacttcc catactccaa accaccccaa
    52921 tagctactac caatattgaa aacacaacgg atgaaatggt tcgaaaaaaa atccaaagaa
    52981 taaatgccgt tatcaacact gccagaatta agaataactt caactcatgc gacacctttt
    53041 ccataacggc agtccgtatg tagggcattc cggaaaaatg caattcaatg ttgtgcaatc
    53101 ttccgaaagc atcagcctcc ttttttatct cattaacaat atcaatacga tgtttgctgt
    53161 caaggtcttt cttgtgaaag gttattgcca tgacagttgc atgagtttcg cgatttaaaa
    53221 tcaatccatc ataaaatggc agttcgctaa ttcggttttt aatgctgtca atttcagact
    53281 ggcgttgtgg caaagtttta actaatggaa caaaatcaaa cttatgcaac gaatcatttc
    53341 tgacaacatc atacaaatcg ccaatagaaa gcacattttg tatgccatcc atttttctga
    53401 ttttatcgga aagtaaaaac caactgttga atttttctat ttgaaaaatg gtactgtctg
    53461 caaaaccaat aaccataaca ctaccatctt ctccgaactt ttgcttaaaa ttttcatact
    53521 caacataagc agggtcggtt gttggtaata cccgaacgaa ttcataagac agtttgatgt
    53581 ttaatgcttt atatgccata aacactgtca acaatagcag tactactata aaagcaatac
    53641 ggtttcttaa tatcttattc gctaaaaatt tccacatgaa tggggcaaag ataagtaaag
    53701 caacttttat agtcagttag gcatcaaagg cctatttgaa agccggcagc aaaaatatat
    53761 tcacctgcaa aaaaatgatg atgtgcacgg ccatcaccta cagttaaaac atttgtaaag
    53821 ttagcgaatg caccttttaa acttgtttca acaaaaaaat atttcattaa atcatagcga
    53881 aatcccgcat caataccaaa cacatagcct gcaatatgaa atttattatc taatctgtta
    53941 ccaaatatgg ttacatctgt tcgtgggatt accaaaccac caccggcttt caatatcaat
    54001 tgcaatctat gattttttga agccgatgaa agaatacaat agcgcttcat cagagtagcc
    54061 atcaaaaagt tggcaccatt cgtatgctca aacatcagga agtctttgcc aaccaaagta
    54121 tctttatcat aaaacgtacc attgatgttg cctttaagat gtaaattctg attgttggtc
    54181 ataatatatt tggtgtgatc aaatgaaatc tctacaccta aatcatgttt atcaccaaaa
    54241 aaataaccgg cacgataaac atattgaggg atagaaatgg ctgaaccaaa gatatatttc
    54301 aaccccggct tatcgtgtgc cttcgcatca tataatgtga aatcataacc accaaaatca
    54361 ttacctttaa aatgcagatc gctctttgaa taccaatcga ggttgtagcc ccatgtcaga
    54421 taaaatgttc ctttgccatg tttaaataaa ggcgtgcaat gacaatcttt ttcaccggca
    54481 aaacaattta cagaaatcaa gaatgccgca caaacagcaa taatcttttg catattaata
    54541 tcaaataaat ggcgcaaagc tacatgaaaa tcaaaaccat tttcttaatt ttatgtttct
    54601 ttgcataata tttggaattg agaatgatga gtgatataat tttaaaatca tctgttgacc
    54661 cgttgttgaa agcaattgca cagaaagtta ttaatgaaga gagaattaca cctgaagaag
    54721 gaataatact ttacgaaaaa ggcgaacttg gttttttagg aagcttagca accttagtaa
    54781 gaaaccgcaa aaacaaaaat ttcacttatt ttaataagaa tattcatatt gagccaacaa
    54841 acgtttgtat tttttcttgc agattttgca gttatagcaa ggactacaaa caccgcgaag
    54901 atggctggga acttactatt gatgaaatgt taaataaagt gagggcatat ccttcaggaa
    54961 gtattaccga agtgcatgtt gttggtggtg tacatccaaa aatggattta cattattttg
    55021 ccggtttgct aaaagaaatt aaaaacatca acccttcttt acatataaaa gcatttactg
    55081 ctgttgaatt ggactatatg tttcgcaaag caaaaatgac aaatgcacaa ggacttcaat
    55141 atttgaaaga tcatggatta gatagtattc ccggtggtgg tgcagaaatt tttgatgagg
    55201 aaattcgcaa taaaatctgt gcagataagt gtaatgctga ggagtggttg aatattcatc
    55261 aggaagcaca tcaattaaac ataccctcaa atgcaacaat gttgtatggt cattatgaaa
    55321 gctattctca tcgtgttgac cacatgaaca gattaagaaa tctgcaggat aagactaagg
    55381 gctttcagac atttattccc ttaaaattca ggaataagga taatgagatg agccatcttt
    55441 ctgaaactac tcaggtagaa gatttaaaaa attatgctgt tgcccggatt tttttagata
    55501 actttcagca tgtaaaagct tattggccca tgataggcag agccactgca caactctcac
    55561 aaaattttgg tgttgacgat atggatggca ctattgatga cacaacacgc atttatacaa
    55621 tggcaggttc tgaggaacaa aatccaactt taaccacgca agaattagtt gatttgattt
    55681 cgcagaatgg ttttgttgcc gttgaacgcg atacactgta caatcatttg aaagtttatg
    55741 aaaatgttcc agtagctaat tgaaagattt attgtgcaga ataatactga gccattaaaa
    55801 aacaacataa ctatagtagg tgcaggccca gcaggtgcaa cggcatctat gtttctatct
    55861 tcctttggaa tcaaacatac tttgattgat aaagctgttt ttccgcgcga caaaatttgt
    55921 ggtgatgcat taagcggaaa agttctacct gtattgaaga aacttaatcc cgatttaatt
    55981 cagcagctat tagtacaaaa cgaaaaatgt ctgggaagca atggaatttt atttggtgca
    56041 cccaatggca aaacattaga agtacctttt aaagccgtaa actcaacttt aaatcattca
    56101 cccggatatg tgagtaaacg aattgatttc gatttttcat taataaaatt attgaacccg
    56161 gagtattgta atttcctgca aggccatgaa gtaaagaaaa taacaaaagg cgaagaaggt
    56221 gtcacactga atgttttaaa tacacagaat aaagaaactt acaatgtaag ttcatcttta
    56281 gtcattgcct gtgatggtgc acatagcatt tgcaataaat tatttcctga tcacaaactc
    56341 gatgcaggac actattgcgg aggcgttcgg gcatactatt ccggtgtgac agggtttcat
    56401 agtgagaatt ttattgagtt acattttctg aaagaaatgc tacccggata cttctggata
    56461 tttccattac ccaatggcat ggctaatgtt ggtgcaggta tgctgacacg aaccatcagt
    56521 tcaaaaaagg taaatctgaa agaagctatg cttcttgcca ttaaaaataa tccttccata
    56581 aaagatcgtt ttaaagatgc aaacattata ggcaacattg atggatgggg attgccatta
    56641 ggttctaaga aaagaaaatt atcaggcaac agaattattt tatgtggtga tgcagcaagc
    56701 atgattgatc cattcagtgg tgagggtatc agcaatgcca tgtactacgg catgcgtgcc
    56761 gcagaagtgt cgcgtgatgc aataaatagc agcaactata ccgatgattt tttatcaggt
    56821 tatgatcagt ttgtttataa cagactttgg aatgaattaa aattaagtca tacacttcaa
    56881 aaactttgca attatccttc gctgtttaat tttgtagtca ataaggcgtc aaaaaacgaa
    56941 accttccgtc agaccataag ctgtatgttt gatgatatag atttaagaga gaaactacgc
    57001 aagccatcct tttattttaa aatgcttttt ggctgatagc aacaatttag tttctattgc
    57061 aaatactctt caataaaaaa gccccttgtg gggctctttc ttatttgatt ccaaaaactt
    57121 tcttcgtttc gttaactgca tttaattctt cccaaggaaa aagttttact ttaatcttct
    57181 tctcgttcgc ttgtccttta ttgtaaacct tttcaaccgt tttagaatcg cgacccatgt
    57241 gtccataggc tgcagtttct aaataaattg gtgaacgaag attaaaacgt ttctctataa
    57301 aatatggacg catatcaaac tctttcatct ttgaaagttt gtttgatatt tctccgtcag
    57361 tcatttttac ctttgatgtg ccataggtat ttacatacaa accaacaggc tgtgcaacgc
    57421 caatagcata agcaacctga acaagaactt cgtcacatac accggcagct acaaggtgtt
    57481 ttgcaatatg tcgtgttgca taagccgcac tacggtcaac ttttgaagga tcttttccac
    57541 tgaaagcacc accgccatgt gcgccttttc caccataagt atcaacaatg atttttcttc
    57601 ctgtaagtcc ggtatcacca tgtggcccgc caatcacaaa ttttcctgtt gggttaacgt
    57661 gatatttaat tttatcatta aacaacacct gaactctctt aggaagtttt ttaagaatac
    57721 gcggaattaa aatattgata acatcttgtt taatttgttt ttgcatggct gcctcttttg
    57781 caaagtcgtc atgctgtgta gaaataacaa ttgcatcaat tctttgtggc ttgttatcat
    57841 cactatactc aatagttacc tgcgattttg catccggacg aagatatttc atcacttttc
    57901 cttctctgcg aattgcagct agttcacgaa gtaataagtg tgccaattca attgctaaag
    57961 gcatgtaggt atctgtctcg cggcaggcat atccaaacat cattccctga tcaccggcac
    58021 cttgttcttc gggctttttg cgttctacac cctgattgat gtctgtgctt tgttcgtgaa
    58081 tagcagaaaa cacaccgcaa gaatgggctt caaacatata ctcactcttt gtataaccta
    58141 tttctttgat aaccttacgg gcaatatcct gaacatctaa ataagcatta gactttacct
    58201 ctccggcaag cactacctgg cctgtagtta ctaatgtttc gcaagcaact tttgatgttg
    58261 ggtcgtatgc taaaaaatta tcaattaaag cgtctgaaat ctgatcggct actttatcag
    58321 gatgtccctc tgatactgac tctgaagtaa ataaatatgg catgattgaa attttgattt
    58381 aaggctgcaa aagtaaccat atttatgaaa tcaaaaatca aatctcacaa taccttttct
    58441 gtgttattaa caaaacataa gagttacaca cttttacagt caattatgta tccaaatgac
    58501 aattatcatt cactaaaaac aaccatcttg ctatgaaatc ttactacaca tttttaattc
    58561 agttaaaaag ccaaaaaatc aaacaccaag gatttgacgt ttctgaatgt taaactcttc
    58621 gtctgtcaga taacctttct ctttcaggcg ggcaagtttt tcaatctggt cgagcttaaa
    58681 atccaatgca tcatgcgagg cagaaaaaat tccttcgggc gaaactttta ttacaggctc
    58741 cggctttact tcaacaggtt ttgcaacagg cacctgttgc aaaaccctac tatgttcaac
    58801 tcttgctaaa ggtttttcgc tctcaggtgt gttcagcaac atttttttag caacaggttt
    58861 tctcagtaga aaaaacaacg agagaataag tccaactgct actaatgagt tgaaaacaat
    58921 aagaacaata tttttctcat tcaaggcttt gttgtcagcc attaatgcat caaagcgagc
    58981 ctgcatggtt ttattagcta caatcaggct atcggattta ttcaaaagtt ggtcaaacgc
    59041 tccatcttta gcttttaagg catcgttgag gttggttaac ctattttgtg caataaaaag
    59101 tgcatcataa atactgtcgt tagcaacagt taccgttttg ccatgaacag tttttatgat
    59161 ggttttgggt ttcaggttag cctgtttttc gagatccttg ttctcattct gcaacgtatt
    59221 tacagtatag tttgcagaga gaataatttt atcgtcaagt tcaagcagct tccgtattaa
    59281 agcctgaaat tctgctgtgg catttctgtt cttaaaagcc ttgtcagacc tgagcatttc
    59341 atcaatctgt ttctgaacat ttttacgttc tacaacaaaa ttattgtatt cgttttgaga
    59401 aaatgctgtc aggctaaaaa ctaaacacag ccaaattgta aaaagagctt tcatcaatat
    59461 taatcttttt ttctaaaacc ttacattttt cgtgccacca atgtcagaac agtttcaaca
    59521 gctacggttt ctccacgttg attggttact tcaacatccc attcaactac accctgaggc
    59581 acctgccctt cacggtcttc tttcttggtt ttcgacttac atgttagtct tacctggatg
    59641 gtatcgccaa cataaaccgg ctgtgtaaaa cgtaaactat ccaatccata atttgcaagt
    59701 acaggtcctt tctttgggtc aacaaacaaa cctgctgctg cagaaataat gaagtaacca
    59761 tgggctaccc ttctttcaaa aattgagcct tcgagagatg tttcgtcagt atgtgcataa
    59821 aaattatcac cactaattcc ggcaaagttt acaatatcag cttctgtaat ggttctttta
    59881 tgtgtgatta atgtgtcacc aattttaagg tcatcaaaat attttctgaa tggatgtata
    59941 atatcttcaa atgtttctgc acctttcagg tatgtattac agatatttga aagtgttgta
    60001 ggtgagcctt gtaaagctgt tcgctgcata aaatgcttta cacctctgat gcctcccatt
    60061 tcttcgccac caccggcacg gccggggccg ccatgaataa gctgtggcat tggagaacca
    60121 tgccccgtac tttctgcagc acaatgacga ttgataacta acattcggcc atgataggca
    60181 gcagtattca atacaacctg tctggcaata ttatcatcag cagtgaacaa agaacctact
    60241 aagcttcctt tacccatttt agctaatgtt attgcttcac cagtattatc ataaggcatt
    60301 attgtggtga caggaccaaa tggttcaatg gcatgtggct ctgcagttga caatggtttt
    60361 ttgcaataca aaagcattga aggcataaaa gcaccttttt ctttatcggc accggttact
    60421 tcaaaatttt taagatcacc aaaagccaca tcacaaacag tcattagttt agctacctgt
    60481 gctgttacat cttttacctg actcttactt gccaatggac ccatgcgtac tccttcaaca
    60541 gaagggttgc ctaagcgaat atctgtcagc cgttttttaa gcgctgtcgc cacttcatca
    60601 gcatattttg caggcactat tgttcttctt attgccgtac atttctgtcc ggacttagca
    60661 gtcatttcgc gtgcaacttc ttttataaaa agcttaaact cttctgtatc tggagaggca
    60721 tcctgcccca aaatacagca atttaaagag tcggcttcca tggtaaaacg cactgaatta
    60781 tcaataatag caggcttctg acgcaacatt cttcctgttt ctgcacttcc ggtaaaagtt
    60841 actatatcct gacagtttac atggtctaaa atatcacctg cactgccaca aatcaactgt
    60901 aaggcacctg caggtaatat gcctgaagca ataatttctt tcaccatcaa ttccgtaaga
    60961 taggaagtag cggtggcagg tttgattata gcaggcattc caccaacaat tgttggcgcc
    61021 agtttttcaa gcattcccca acatggaaag ttaaaagcat tgatatgaat ggcaactcct
    61081 tcaagaggca cacagatatg ctgtccgata aaagttccat tcttagataa tggttcaata
    61141 tttccttcta ctaaaaaagt ttcgtctggc aattcgcgtc tgcctttact ggcataggca
    61201 aataaggtcc caatgccccc ctcaatatca atccatgaat cgttgcgggt tgcacctgta
    61261 gcaccagaca actgataaaa catttctttc ttagccatta agtgcatggc taatgctttt
    61321 agttttcttg ctctctcatg aaaagtcatt gcgcgcaatg caggtccacc cactgttcgt
    61381 gcatagtcta acatggcttt aaaatctaac ccctcagaag aagctatggc tatttgttct
    61441 cctgtaactg catgaaacaa tggagttcca cttccggtgc cttccaccca ctgtccggca
    61501 atataatttt ttaatctcat ttgctgttaa aaaaaatgtt ttattcgaag gcgtgaaatt
    61561 aaagaatcta accacatata acatgataat tagctgctga atttcaagaa cagaatgtgt
    61621 ataaccctta aattctattc ttaacaacta ttgttaatca aacattaata attcaatagt
    61681 tttcaatcat tctttaccct atttttgccc attgaaaaat caaattattt ttcagcagta
    61741 ttcttactga tttttaacaa ttatgcggaa accaaaagtt ttaatgcttg gatgggaatt
    61801 ccctcctgta ataaacggtg gactgggtgt tgcctgtcat gacctatgtc tggctatggt
    61861 taaacatgca aaagtgtcta tggttatccc taagannnnn nnnnnnnnnn nnnnnnnnnn
    61921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    61981 cttaaacaag ttaactacaa agaagaatac aaaacttttg aagatgtaca ttttattcct
    62041 tctgctttaa atccatacta cacagacaga aaggcagctt tcgaaattcc gggtattgat
    62101 gttgaatcgc aatacatgaa agaagtaaag attggcaatc gtcatgcaaa agtttttcat
    62161 attgatgatc tttatggtgg tgatgttatt gagaaggtaa cagtttttnn nnnnnnnnnn
    62221 nnnnnnnnnn nnnnnnnnnn nnnnncactt acattggatt ttgatattat ccatgcgcat
    62281 gactggatga ccatgttggc aggaatggaa ataaaaaaac aaagtggtaa gccattggtg
    62341 gtacatgtcc attcattaga agttgaccgt ggcggagtaa acagccaagg gtgggtatat
    62401 cagatggaaa aaagaggtat gcaggaagca gatgtgctca tgcctgtaag taactttacc
    62461 ggaaatatca ttaatgaata ttacggtatt gactacagaa aaatatttcc tgtacacaat
    62521 ggcatcagag aggtgaaacc tttcaggagt aaaagtcctt ataaggagaa aatagtactt
    62581 tttgtaggac gtttaacaag acaaaaaggc cctgaatatt ttgtagatat tgcagccaaa
    62641 gtcttagaga aaaatcaaaa tgtgcgtttt atcatggcgg gcacaggtga tgcgcaaagc
    62701 agaatattag aaaaagttgc tcagaacaga ataggacatc gctttcatct tgcaggattt
    62761 cttggcttag acaaagtacg ctacctgctt tctattgctg atgcctactg tatgccatcc
    62821 gtgagtgagc cattcggact ttctgctgtg gaagccgtac agtttgatgt gcctgtcgta
    62881 atcagtaaac aatctggtgt atctgaagtg ctttccggtg ctctgaaatt tgatttctgg
    62941 gatatcaatc aggctgctga atatttgctt ggtttattgg ataatgacac actccacaag
    63001 caggttgtta aagatgcaca aaaagacctt gaagacatca gttgggatat tgcagctaac
    63061 aaggttattg aaggttacaa gaaatacaaa ctgatttaaa atttttaaaa ctttattttt
    63121 aaaattattt ttgcaaaaac aattcaacat aaaattataa atatgaacct acctgctgat
    63181 ttgaaataca ccaaagaaca tgagtggatt aaaatagatg gcgacatagc tacaattgga
    63241 attaccgact ttgcacaagg cgagttgggc gacattgtat atgttgacat caacacatta
    63301 aatgaaactt tgaaaaaaga cgaagttttt ggaactgttg aagctgtaaa aactgtgtct
    63361 gatttgttta tgcctgttga tggtaccatt gttgaaataa atccaaagct tactgatgca
    63421 ccggatagtg taaataaaga tccttatggt gatggttgga tggttaaagt aaaaatttct
    63481 gacacctcac aacttagtgc acttctttct gccgatggct atgctgccat tgcaggtgcc
    63541 tgataaattt taatgattcg tcattatatc ccacccttaa tgtggggttt attcattttt
    63601 attctttgtg ccatacccgg taaaaatttg cctcattatt catgggcaga tttgttgtcg
    63661 gtggacaaat tggttcattt tacattattt tttattctca ttttactctt aaaaagagcc
    63721 tggcttttta tttataaggg agtgagttct tataatctaa ttgcaatagc aattggcgtt
    63781 atttatggag ggagtttaga actgatgcaa tcctactttt acacagacag aagtggtagc
    63841 tggtttgatt ttttagccaa cagtttggga gcaatatgtg gtgtgctttt ctttgaaaaa
    63901 ctgatagata aaagtggacg atggaagttc aatcttggaa acaagtaatt attgtttcac
    63961 aaagactgct tttatttcgc tgatgattaa atcatccata ctcattattt tatttttgaa
    64021 gcattgcgga tgtttaatca ccaagtccag aaaataagga gaacgaaact ctttgtaata
    64081 accaatttta cacttatccc agtaagtctg tttaaacaac tttctgaatt ggtctatatg
    64141 ccatcggttt agcgttgcat ctatattgaa tttctcatca gcatgagtat tgatccactt
    64201 attcaaaaaa tgcttatcaa acaaaaactg acaatagggt atgccaacat tattataggc
    64261 atgtgcacca taaggagaat gatataaagg cgcaaagtga ataaacatta aaccgcctgg
    64321 ctttaacact cgcagtgatt cttcaaatac tttttgtggg tcaggaaaat gctcgaaaca
    64381 gttatatgaa aaaacaaagt cgaatgaatg gtcttcaaac tgaagttcgg cagcatccat
    64441 tttcatcagg ttcacacctg actcctgtgc tcgttggtca aaatcatcat ctctgaattc
    64501 tatggctgta acttgcttgc cacgttcttg taatgatgaa cttaccatgc catctccggc
    64561 acccaattca agtatattat tgtatgatgt cctggtgtaa ttatgcatta taccgattaa
    64621 atcttcggca cgttgaatcc ctctttctct tattgaaacg gcatcataca tcacaggatc
    64681 accaacagga tactggtcaa ataaaggttc aaaatatgtc tcatcaagta cttccgaagg
    64741 acctttaacc attgcaattg cccgggcagc tttaaatagt ttctgctcat aataaaaatc
    64801 cgataccatt tgtctggtac catctggtaa aattcttttg gcaatattgg ctaacacttt
    64861 ggctcaggtt taatgcgccc aaaagtaaag taagaaatac ttttttctta aattgttaat
    64921 aagttttaac cccagtggga acagaattta ttgttgtgaa ttgtaatcgt attaatttac
    64981 aagactttta agccatacta aagtcagttt ggatgattga tatcaatttt ggacaaacgc
    65041 atttgtttat aaaattacaa cagaaataca attcaatcca catagtatca cggactgaat
    65101 atactattct acgtaacatt tcactctgac aatcgtacag atggaacgaa gaaatgacat
    65161 tattggctgc ataaaaacaa aacttgattg aacaaatgct ttgatatttc ttcatcaaaa
    65221 attatctttg caaaaaatat tactccctcc tattttacca attgaatgaa cagagaagaa
    65281 atagtagcaa caacaaaacg ttttttatcg gaagaatttg aagtagatga aactttaatt
    65341 gaagaccaga aaaatctgaa agatacttta gaattagaca gtcttgacta tgttgatctt
    65401 gttgttgtaa tagaagagaa tttcggtttt aaaacttccg gagaagattt taaaagcata
    65461 agcacgtttg aagacttcta caattttgta gagcgcaaac ttgcagctat ggcctgataa
    65521 aatggcacag tgggacggcc gtagcagagg cactgtttta ggttatcgca tttttgtttt
    65581 catgattcaa tatggtggag ttagggctgc ctattttctt ttgcgatttg ttacttacta
    65641 ctatttttta ttcgcagcaa aaccaaagaa aatcctcata aatttttatc aaagagcctt
    65701 aaaggtcaat gaagttgttg caaaaaaact atgcagaaaa aacttttact acttaggaca
    65761 gaccttagtt gaccgtacag catttttact tggaaaaggc aatgttttca gtcataattt
    65821 tgaaaacgag aaatttcttg tcgaaatgca ggaacagaga aaaggtggca tactcatcag
    65881 tgcccatgtt ggcaactggg aaactgccgg aaaccttctc aagtcaagag tttcttcaac
    65941 cattcatgtt ctgatggttg atgcagaagc taaaaaacta aaacaatatt tagataacag
    66001 caccggtggt tcgcaattct ccataattgc tgtgaaggac gacctttccc atattgtaaa
    66061 agttaataat gcattagccg ccaatgaact cattgccatg catggtgaca gatacttgcc
    66121 gggcacaaaa accattcaat tagatttcat gggccgaaaa gcccattttc cctacggacc
    66181 gtttttgctg gcatcacgtt ttaatgcacc cgtcactttt gtttttgctc ttaaagctaa
    66241 tgattttcac tatgagttaa gtgctaccaa acccataact gaaaagaaat cacctgagga
    66301 tattgctgcc ttgtatgttg aagaattgga aaaaaaagtg aaaaaatatc ctgaacaatg
    66361 gtttaattat tacgattttt acagcgaatg attattgaaa aagtaaatat cgaaaactat
    66421 attccacagc gctttccttt tgtcatgatt gacaatctta ttcatgccaa tgaatcagga
    66481 tttttaagtt catttctggt aaagaaagaa aatatattta gtgaaaatgg atatttgaca
    66541 gaaccggcat tgatagaaaa tattgcgcaa acctgtgcag caggttttgg atttattggt
    66601 cagcaaaaaa atgaaggccc atctattggt tacataggtt ctatcagtaa acttacagta
    66661 caccaattgc ctgaatatgg aacaaccata ttaacaacag ttgaaattac aaacacactt
    66721 ggcaatatat ttttaataaa gggtaaaaat acttgtaatg agaaattatt ggtagaatgt
    66781 gaaatgaaaa tagctcttgc atgaaacaac ttttacaagt agaaattccc attaaagtaa
    66841 gattcagtga taccgatgca atgggtgtgg tatggcacgg aaactatctg cgtttttttg
    66901 aagatgcacg cgaggcattc ggaataaaat atggtctcac ctatcttgat gtttatcatc
    66961 gtggtttttt cactccaatt gtcaaatcat ctgttgaaca taaatcgcca attaattatg
    67021 gtgaagaagt tgttgcaata gcaaaatttc accctacacc atcagcaaaa attatgtttg
    67081 attttgacat cctgaataaa accacaggaa aattaagtgc taaaggtcat accacacagg
    67141 tatttctaag tgtagttgag cgcgaaatgg aattggtgaa accaccattt tacattgaat
    67201 gggaaaattc tataggcatt aaacattagc aagtgatata catagggtcg gaaaccatac
    67261 ttagtcctaa tggcaatgtg gtagattgtt ttaaaaagat tgccgaaaaa aaatcaggtg
    67321 ttagcaagat accaggaaat ggttttaagg gagagtcttt ctattgttct atgtacttag
    67381 agaacagacc ggactttaac acattagctg cacaaactat tgaacaatta ttgcaaaaat
    67441 cgaaagccaa tctgcttact gaagaatcta ctcttcttat catcagtacc accaaaggcg
    67501 atttatcaaa aggatttgaa aatgcaataa tcagtccggc aaaattcctt tacgaaaaat
    67561 taaaactaaa acattttcca ctcatcattt ccaatgcatg tgcgtctggc gtcatggcaa
    67621 tcaatgcagc agcagcgttt attgaaaaca aacaatatca aaatataatt gtacttggta
    67681 ttgacatgat aacagacttt atcgtttatg gctttcaatc tctttatgct attagtgatg
    67741 agcattgcaa accgtttgac aatgatagaa aaggaattaa tttaggtgaa ggtgccggtg
    67801 cagttttatt atctgcaagt aaagatgttt ttacttcaac acctgtttgc tttttaggtg
    67861 gaagttcaag taatgatgcc aatcatattt cgggaccatc aagaacaggc gaaggattat
    67921 ttcgcagtat aaataaaaca tttaattcgg ctggcattaa gacggaagat atagatttta
    67981 tctctgcaca tggcacagct acagtgttta atgatgaaat ggaagcaata gcttttaaca
    68041 ggttaaatat gcaacacatt ccggtaaaca gtttcaaagg atttacagga catacacttg
    68101 gggctgccgg tgttattgaa acagctttat gcataacctg tatgcatgaa aacaaattac
    68161 ttccttctgc cggttattat aacaaaggga caaccgttga gcttaatttg ataaaagatg
    68221 ctgcttctgc tacaataaac actgtcttaa aaacagcatc cggctttggc ggatgtaatg
    68281 catcactatt attgcaaaag ataaaatgaa aataacaggt tgctgtagaa ttcatcaaaa
    68341 cgaagtttta gttaatggta aaaaatattt tacctctgac aagaatcagc atgaagagct
    68401 atcaatgcag ctttaccgat tcctgaaaac agagtatcct aagttttaca aaatggattt
    68461 gctctcaaaa atgggatggc ttgcaacgga aatagtttgt cagacaattc ctacgatatc
    68521 taaatttgct gacgatgaaa tcgcattgtt atttgccaac agccactctt gttcagaatc
    68581 agatgagcga tttaatcatt cctttaaagt agagaatagt ccttcacctt ctcagtttgt
    68641 atataccctt ccgaacatac ttttaggtga gttggcaata agacataaat ggtatggcga
    68701 aaatttgttt accatactac ctgaattttc tgccgagcat ttcagttctc atgcaggtat
    68761 ttttattcct caaaaagcaa aagcattatt ctgtggatgg ataaaccttt ttcctgtaac
    68821 agatgtattt gttttctttg cagaagctaa ttcagaaaca tcaccgggaa ttttggatag
    68881 taaagaaatt gaacattggt ataaaataat aaagtgaaat ggataattta aaagaagaat
    68941 taaagaagca tttaattaca caactgaatt tggaagataa aacaccggca gacatcagtg
    69001 atgaagctcc tttgtttggt gatagcggct tgggactcga ttcaattgat gcattggaat
    69061 tgatagtact tttagacaaa cactatcaca ttaaactcac aaacccagaa gaaggaaaat
    69121 ctgtattcta ttctatcagt accatggcag aatacattga gaaaaataaa aagtaaattt
    69181 cgatgaaggt ttatgtcacc ggcattggtg tagtttctgc aattggttta acagttgaag
    69241 aaaattatca ttctttatta aataagaaga gcggtataac tgcatttaaa ttcccggaag
    69301 aacaaaaatc tattcaagca ggaagcattg caaaatcaaa tgctgaactt aaacagatgt
    69361 tatctataac taatggtaat ttttcaagga cttcattact tggaataatt gcagccgaac
    69421 aagctatggg taaaaacttt cacaataaaa aattacgtac cggttttatt tcatctacat
    69481 ctgttggcgg tatggataaa acagagaagt attacaggca ggtcttaaag aatctacctg
    69541 ttgattttaa tctgacacga actcacgata gtggaaatac ttcagaaaat attgcagctt
    69601 acttaggatt ttcaggttac attaatacac tttctacagc atgttcatca gccgctaatg
    69661 ccataatgct tgcagcacga atgatagagc aaggaaaaat ggacagagtt ttagttggtg
    69721 gaactgatcc gataacagat tttacaatta aaggctttca atcgttgatg atatatgatg
    69781 atgaatggtg caagccattt gatcataaca gaaacggact taacctaggt gaaggcgcag
    69841 gttttttatt gttggaaagt gatcacagca taaacatttc gggcaatgaa ccaatttgca
    69901 gtttaacagg atggcataat gctgccgatg cctatcatca gactgcttca tcacccgatg
    69961 gtaaaggagc cacaattgca atgaaaaatg cattgaagaa agcagggctt gggcctaaag
    70021 aaatttctta catcaacgct catggcacag ggactaaaaa caatgatctt agcgaatcca
    70081 ttgcaattaa aaatgttttt ggcgagtcta taccaccttt tagttcaacc aaggcataca
    70141 caggtcatac attggcagca gccggtgcaa ttgaagcagt atattctgtt cttgcaataa
    70201 aaaatcaaat ccactttgca ggattaaact acaatgaccc tttggaggaa acactcatgc
    70261 atcctgttac agaaccaaca aaagctgtaa caaaacatgt gatgtcaaac tcttttggtt
    70321 ttggcggaaa taattcttca ctaatttttt caggcatttg attatgtatt gtattcaatc
    70381 tgcttccacc atttctcatc aaccttcgtt tgcaagcaaa gggttttcct cgaatctttc
    70441 taaagaaatt gaatcattaa ttaaacctga ttacaaagag tatgttccgc ctgcattaat
    70501 cagacggatg agtgaaatac tgagaatgtc tgttgcctgc agtaatcttg cattaaaaga
    70561 agctggcatt gaacaacctg atgcaataat aaccggtaca ggattgggtt gtctttatga
    70621 cactgaaaaa tttctaaaca atgtaattac cattgaaggc gggctgctcc ctcctacttc
    70681 attcattcaa tctactcaca acactattgc aggtcagatt tcattggttc ttggcaacca
    70741 tcaatacaat atgacacaca ctcaaaacag tctgtcgttt gaacatgcgt tgctcgatac
    70801 tatgctaatg cttgatgaaa aaaatggaaa tattcttatt ggcagtgcag acgaggatat
    70861 tgaagcatta catcctattg cagcacattt taaattaaac tcgttaccac ttacaagtgg
    70921 tgcttcattt tttgtcttat caaaaaataa aaacgaaaaa actttagctg ttattgaagc
    70981 atgtcatact tctgcttttt attcatcact tgaaaatgaa attaaagact tcatgagcac
    71041 atcaacagat aaacaaatag actttatttt gaatgatgga cgtaataaag atataacaag
    71101 attaattgaa acattatttc cagataagtg cgtcattaat tattctgaaa tttgcggatt
    71161 gtatgctaca aattctgcct ttgcaatgca tctggcaact gatataatct gcaataagac
    71221 aaaacaagtt tatggcaaaa caattgctaa ccataaacgg gctttaataa taaacacctt
    71281 gctacctgca cgggccggat tcataatgat aaaaagcaat gaagcataaa atcgtaactg
    71341 taatttttat tctctgcatt attgcattat ttgttttcct ttatcaggac aaatcctttt
    71401 tttatttatt ggcggcactt attttccttt atctgacaat tacagcattg ggctcctatc
    71461 agattaaatg gaactatttt ataaagtcag ttaatacagg aaaacctgaa ggtatatcac
    71521 ttacatttga cgatggacct gatccaaaag tcactaaaaa aatattggat gttcttgaac
    71581 tggcaggtgc gaaagcaaca ttttttgtga ttggcaaaaa ggctgaacaa tatccggaca
    71641 ttattaatct aattgtcagc aagggccaca ctataggcaa ccattcatgg tcacatcatg
    71701 cagggattgg catgttttca aagcaaaagt tgttggctga ttttaatcaa tgcagtcaaa
    71761 aaatagaaga gcttacagga caaaaaccag tttatctaag accaccattt ggagtaacta
    71821 atcccagata tgagtatgtg ttgaaaaata caggattgaa atctattgga tggagtatca
    71881 gaagttatga cacctcagca aaaaatgagc agaaactttt acaccggata acttcacaat
    71941 tgaaacccgg agcaattgta cttttgcatg acacaaaaga tataacactt gccattttac
    72001 cggcattgct aaactattgt aatcaaaata acattgccat taaaccgtta gctgaattaa
    72061 ttgaaatatg agattgttaa ttgcatttat actattcatt tggatacctg tttttgccaa
    72121 agcacagcag gaaccattga acaatccggc tgagttaaca gaaaaactca agcaagcaag
    72181 catttcaaac aatacaatta aagcagattt tattgaacaa cgatttatgt ctgcattgaa
    72241 agagccacaa acatcttatg gtatttttta ttacaagaaa gaaaacaaat tgcgatggga
    72301 aaagacaaaa cccacaaatt atgtttttat cagtgagaat aataaagtac gtgtgaagga
    72361 aaataatcat gagaaagatg tcagttccta caatcaggtt gttggaagaa ttaaagattt
    72421 aatgctgact ttggttaatg gtgattttag taatggcaaa caattcgatc cgcagtattt
    72481 tcaaaccaaa tcagaatata ttgttaaatt aaagccaaga aacaaaagaa tgagcaatgc
    72541 atttgattac atacaacttt cattcaacaa aaagaattta ttacttgaag aattggcttt
    72601 ctatgaaaaa tcaggtgaca ggaatatcat gaagttttca aatcaaatgg taaacacaga
    72661 actgaacgac acattattta aaaacttctg atgtgctgaa agatttctac accatcatca
    72721 ataaaacatc cgggcaaaat tgtgtgtatc ggattaaact aaatcctgca catgaaatat
    72781 ttaaaggtca ttttccggga caaccggtta cgccaggtgt ggtattaatt caaatgacaa
    72841 cagaactcac acaaaatgca attgagcaaa atgtaaagct gaagcaagtc attaacagca
    72901 aatttttaaa tgtaatgaat cctgaaaaga atcctgaagt tacatttaaa atcagtatta
    72961 ctaaagatgg tcaattatac aaagtagcaa catctgctga agacgagggt gtttgttttt
    73021 ttaaaatcac cgccatttat cagtgaataa attggctgac atagctgcat gtgttattat
    73081 accaacatat aataattcca aaacgctaaa gaaagttatt gatggtgttt taaatttcag
    73141 ctcaggaaac gatgttattg ttatcaatga tggttcgaca gataacacaa cagaaattct
    73201 taatcaatat aatgagcaaa ttgttttact aaacaacaaa acaaattccg gcaaaggtta
    73261 ttcgttgcgt aaaggtttta aagaagcaat ttcaagagga tttaaaaatg caattaccat
    73321 tgattctgac ggacaacatt atcctgaaga cattgatgta atgattgaga atgccctaaa
    73381 atttccggaa gcattattga tgggttcaag aaatatgaat caggaatcag tgcccggaaa
    73441 aagttcattt ggtaataaat tttccaactt ttggttttgg gttgagacgg gacaaaaatt
    73501 acccgataca cagacaggat ttcgtttgta ccctcttgaa ccattaaaga aaattaaact
    73561 ttatacaact aaatttgaaa cagaaattga agtcatagta aaacttgcat ggcaagatgt
    73621 aaaaataatt ccagtaaata tcagagtgct ttacgacatg aaagaacggg tatcgcattt
    73681 tcgccctttt cgtgatttta caagaatcag tatcctgaat acttatttag taaccctgac
    73741 acttttttat tatttaccat tacgattatt caaatacatc agaaggaagg gaattttcac
    73801 aattataaaa gaagagattt tcagacgtga tgaaagcatt ttaaaaaaat cactttccgt
    73861 tggttttgga gtatttatgg gcattgttcc aatctgggga tttcaattgc ttgtaggtat
    73921 tcccttagca gttttgtttc gattaaataa ggtgctattt ataactgctg caaacatcag
    73981 tattccaccc atgattcctt taattattta ttttagttat tatctcggag gtttcttcgt
    74041 ttcaaacgat atgaaatttc aaagtatggc tgatataaca ttgcaatcta ttcatgcaaa
    74101 ttttatgcaa tatgcaaccg gtgcctgtgt gcttgcttta atctccggta ttttttttat
    74161 gacaattacg tacattacat ttttggcact gaagtttaaa gataagctta cggagaaagc
    74221 ataattctca gatttatgta aaaatttcaa actgactaaa atcattcatt cgtaaaaatt
    74281 aattaaaatt aaacccttat ctatttaatt acgagtgttt taaatgaatg ttcggcatat
    74341 ctaaattcta ataaagtcgc atctgtaaac tgttcataat taacggtagt ttaatacttg
    74401 tattttggaa agaactaatt gatgctatac atttgcacct gtttttagaa caatagttca
    74461 atcgctatta attcagatat tattacaaac aattatacaa tatataacaa tcaaataaat
    74521 acattaaaat gagaaaatca cttttagcat tagcatcagc tgcttttatt tttgcagcat
    74581 gtaatcaatc aaaacaacct gaaactcctg caacagagac aacacctcct gctgcaacag
    74641 aagaagcagc accggcagta gctccaccaa ctgagcctgc aatagttgaa atatccggtg
    74701 gagatgatat gaaatttgac aaaacagaaa tcactgttaa agaaggacaa actgttaagt
    74761 taactttaaa gcacacagga aaatcgccta aagatgcaat gggacacaat tttgtattgc
    74821 tagcatcagg tacttcatta caagatttcg caacagctgc aattaaagca aaagacaacg
    74881 attatattcc tgctgacatg gctagtagtg taattgcaca tactaaaaca attggtggtg
    74941 gcgagacaac agaaattgaa tttactgctc catcaaaagg aacttatgat ttcctttgct
    75001 cttttcctgg tcactatggt accatgaaag gtcactttat cgttgaataa tttaactgat
    75061 aacatttacc cgccaaacat ctgatttgac tgtttggcgg gtattttttt aagtaactct
    75121 cattatattg tttcaataac tcaagacttt taacacaaat tccttcagaa gtcttttact
    75181 tttttagtaa ttcaatttta taaccggcct tctgaacagt attaataatt tcttcttctg
    75241 aaatatcatt tgttgaaaca gacaatattt tatccttgtt agctgtatct acatgccaac
    75301 tttcgctgct gattagttta tctaaatgtg gttttactgc ggcaacacaa ccactacagt
    75361 taatgttggt cttgaattga agatgttttt tgttttccat tttatttaag tattaataaa
    75421 ttgcttttct gtgtgcaaag tttgttattt tgtttgagtg acaggttaca acattataat
    75481 ttttatttgt aaaatatact gctacttttt ccattttaat ctcaaactat tactcacaac
    75541 gctaacacta ctcatagcca tagctgcacc ggcaatcatt gggtttaaaa gaaatccatt
    75601 gattgggtac aaaatacctg ctgcaattgg aattccaatg acattataaa taaatgccca
    75661 aaacaaattt tgcctgattg ttgcaacagt ttgttttgac agccgaatgg ccattggaat
    75721 tttatttaaa tctgaggaaa caattgtcat ttttgcaaca tccattgcaa tatcactacc
    75781 ctttcccatt gcaatactaa catcagcagt agctaatgct gtactatcat taattccatc
    75841 accaaccatt gctactactt ttccttgttg ctgcaacacc ttaataaaat cggccttttg
    75901 atgtggcaac atacttgcct gatatttatc aatacctgtc ttttctgcaa ttgcttttgc
    75961 agtagattcg ttatcaccgg taagcatata aagctcaata cccattttat gtagttgctg
    76021 aatagcagcc actgacgttg gctttatact gtctgaaatt gcaataatgg acaacacttg
    76081 cttactatcg gcaaaccaaa taactgtttt tgattccttt gtccatgcag tagctttttc
    76141 gactttcata tcttctatta aaatattttg ctcctttatg aaagtcatat ttccaacaaa
    76201 ataattctct ttctgatatg ttgctcttac acccttgccg gtaatgcttt caaatgaagt
    76261 tagttctata tcttctgtct tagtgaaatt ctttactact gcatctgcta atggatgttc
    76321 agacttcttt tcaatactat agaggattga tgcagtactt gaattatcat tataccaata
    76381 aacatctgta acaaccggtt taccctcagt aattgtaccg gttttatcta aaacaacagt
    76441 tgtaattttc tttgcaagtt caaggctctc tgcatctttt ataagaattc cattttcagc
    76501 tgctttgcca acaccaacca taattgcagt tggtgttgca agacctaaag cacaaggaca
    76561 agcaattacc aataccgtta ctgctgccaa caatccctgc actaatccat tatcaccgcc
    76621 aaaaatttgc caaagaacaa atgttagtat tgaaatgcct ataactacag gaacaaatat
    76681 ccctgcaatt ttatctacta atttttgtac cggagcctta ctgccctgcg catcctgaac
    76741 agtctttatg atttgcgcca gcatggtttc ctttccaacc tttagtgcat taaactgaaa
    76801 gctgcctttc tgattgattg tacctgcaaa cacgttgtca tttatgtttt ttaaaacggg
    76861 aacaggctct cctgtcagcg tactttcatc tacgtatgaa ctgccattga taagtttgcc
    76921 atcaactgca attttttcgc caggtttcac aagtatgata tcatcaacaa tcacatcttc
    76981 aacgaaagtt tgcttttctg ttccatccga ctgaattaca ataactgttt taggctgcaa
    77041 ccccattagt tttttaattg ctgaagatgt attccccttt gccttctctt ctaacatttt
    77101 gccaagaagt ataaatgcaa ctattacagc agcggcttca aaataaacgt gagcgtgaag
    77161 tcccctttga tgccagaatt gaggaaatag catattgaac acactaaaca gatatgcaat
    77221 tcctgtactc agtgctacca atgtatccat atttgcagag cggtgttttg cttgtttcca
    77281 tgcattgata aaaaaatcct taccaagcca tagaactaca ggggttgaaa acagccacat
    77341 aatttcatta gcataaggta tatccataag aaacatacca attattacaa caggaattga
    77401 tagtgcaata gctaaaacag tcttctgttt taacttttta aacttctcct gctgtatgtt
    77461 ttcaaaacta ttctgttgtg atgcctcatc ttctattagc aaatcatatc caacctcttg
    77521 taatgccttt tgtaatgcca gcggattggt catgtttggg agatattcaa ctgtaagttc
    77581 tgttgtggca aaatttacag aagcattaat tacaccattt accgattgaa tcatactctc
    77641 agcactaact gcacatgaag cgcatgacat atttaatact ggaaactttt ttcttacggt
    77701 tgttacgcca tatccaaggt ctttaattgt tgcaatgaca tctttaatag tttctttatt
    77761 tgaaacagta attacagcac gtctgttatt caattcaact ttatgtgaat caacacctgc
    77821 aaccttggaa agccctttat caactatcag tgcgcaatgt tcgctttcta cattttctaa
    77881 aggaagaaaa attgtgtcat cagtattctt ttccatatca aagcaaattt acactgcaaa
    77941 ggtgcaaaga atgaaatcaa aaaacgttat agttttatgg aaatgaattg taaaatttac
    78001 accttgtcta aaggtttgcg tttgtcggct ttaatttgct taaaatgact tggagttaat
    78061 cctgttacct tcttgaattg attactcaaa taagctacac tagaataatt cagtctgaat
    78121 gcaatttcac ttaatgacaa ttcatcatac actaatagct ctttaacacg ttctattttc
    78181 tgagcaataa aatacttttc gattgtagta ccttctactt ctgaaaaaag atttgacaag
    78241 tatgtataat catgctttaa ctcattactc aatacttctg aaagattctt ttttaactca
    78301 ccatcatgat gatgaaccaa atcaataatt aatgttttta ttttttctat gattcggctt
    78361 ttcttatcat caatcaattc aaaacccaac ttttttaaat tatcctgcaa ttgtttcttt
    78421 tctgcttcat ctggtgttac ctgaatagtc acttcaccca actgaacatc tgttgtaatg
    78481 aagccactat cttccagcaa cttttgaact gccataatac agcggttgca aaccatattt
    78541 ttaatgtaaa gtttatgaga catatttact ttccgataca aaattgacta aagatataat
    78601 caagtaggtc ttcagttgac acttgccctg taatctcccc taaagaataa attgcatctc
    78661 tgatgtgtgt cgaaagtaaa tcattgctga ttccatcttc cattccctga agaaataaat
    78721 ccagagcaga ttttgcctgc gataacgata ttgcatgtct tgcattggta acaacagact
    78781 cattcaaagc aacaccatca atacctgagc gttctattaa tgtttctttc aataaattga
    78841 gatttgattt attctttgct gatatcaata ttaccacacc acatttatca taactatgca
    78901 tatcaaagtt agtacttaat gcatcagatt tatttgcaac aaacaaaact ttcttctgca
    78961 attcattatc agaaattcca agttcattct gcaattgcaa aatttcattt tttaaatctg
    79021 caggtgatga ttccattgga tcaaaaagaa ataaaatagt ctttgcctca tttgctttag
    79081 caaatgttct tttcacacca atgctttcta tggcatcgtc agtcttacga atacctgcag
    79141 tatcaataaa tctaaatcga attccgtcaa cattcaattc ttcttcaatg gtgtcacgtg
    79201 ttgtgccagc aatatcactc acaattgccc gttcttcatt gagcaatgca tttaacaagg
    79261 ttgacttacc aacattaggc ttgccggcaa ttacaacagg aactccattt ttcaagacat
    79321 tgcctaattc aaaactttta agcagatgtt caatcttact ttgcacattt aaaacaagtg
    79381 tttttagttc tgcgcgactg gcaaactcaa catcttcttc actaaagtca agctcaagtt
    79441 caatgagggc ggcaaacttt attaactgat cgcgcagtga ttttatttct tcagaataac
    79501 cgccacgcat ttgctgcatg gctgcatgat gcgaaataca gttttctgcc tcaatgatat
    79561 ctgctacagc ttccgcctgt gcaagatcca tcttcccatt caagtaagct ctcatggtaa
    79621 actcaccggg ttttgccatc acagcaccag attgcataat cagttgtaaa attctttgtc
    79681 tgatataacc tgacccatga caactaattt cagcaacatc ttcaccggta tatgttgcag
    79741 gagatttaaa tatggagaca agtacttcat caatcaaatc atttttgtca actatttttc
    79801 caaaataaat acgttggtgt tctaactctg aagttgacaa agttttatta gctggagtaa
    79861 atatttttga aataatttca aatgttttca ttccagatat tctgatcaca ccaacagtac
    79921 cacgacctgg tggtgtaatt aaagcacaaa tggtgtcagt ctgtaatatc atttttgttc
    79981 tataataaaa ttattaagcc gattagtaag ttgcatgcca aaattagttc caaatacttt
    80041 accctgcatt ttattcaccg gtctgatacc tgagcctgta ttggtaagaa atatttcatc
    80101 aacatcaatt aattgttcta ttgaaatatc ctgttctata gcactagtca gctgaataat
    80161 aactttacgc atcacaccat tcacacagcc tgaagataat ggtgttgtat aaaccttatt
    80221 ccctttcagc caaaacacat tagatgccgt tgcttcacaa agcatgtttt tgctatttaa
    80281 tatcagacag tcatcaaatt tcatttcttg tgcatatatt gatgctaaaa cataaagaag
    80341 gctattacag tttttaaatc cggaaagttt gttaattggt ttttgttgct cactataaat
    80401 tcctaattca ataaaacact taggtattgt atattcagat tctactgcag ttattggaag
    80461 catacatagt gaggcattgt tctgttcagg caggtaagtg cctccggcat ttcgcgtaat
    80521 caacaatcgt agtttgctgc catctgtaaa attatttctt tgattcatct ctaaaataat
    80581 ttcacgtaga taatcttcag ttataaaatc gcacttaaga ttcagaacag caacccctgc
    80641 aagtaagcgt tcaatatgta gtgacaaaaa atttgcttta cccctactgc aacgaatgga
    80701 ttcaaaaagt ccatcgccat aaataaatcc tctattgttt agcttcagaa cgggagttgc
    80761 ggcatcaacc cattgtccat cataatacgc ccacttcatg ctgacagatt tttcttgcta
    80821 attcaacaat attgctgttt gattcaatca gatgaccctt tactcctgct ctgtttgcag
    80881 caatcatgtc tgtttccata tcaccgataa agtgtgcatt attcttatca accttataac
    80941 gtgcaatggc cttttctaac aacaggctat cgggtttgcg gcaaaggcat ttactcttct
    81001 caggatgatg tgggcaataa taataatttg ttatagttat gcttttcttc ttaaattctg
    81061 ctgacataaa ttgatgtact gcaagaacat cattatgagt ataaactcct tttgcaatgc
    81121 cactttgatt ggtaacaaca aaaattaaat aaccattgtc ctgaaatatt ttcatagcat
    81181 caaagatgcc gtcattaaaa acaacatcag caattttata agtataatac aaagtattgt
    81241 cattgattac tccatctcgg tcaaagaaaa ttgctttgtt tgaattaatc atcagagcat
    81301 acctgttagt tgatcaaaga aagtaagtat taatcccggt gcaaatgtta ttggaaaaac
    81361 aaaaccatac acagcacccc aaaaatgcgc attatgattg atgttatctc ctcccttttt
    81421 atccatgtaa ctcgacagcc acaaataacc tacacctgca agaattccag gaatacctaa
    81481 cagcccatag agataaattt tctgtaaggg aaaaaataaa ataaaactaa acagcaccgc
    81541 agacacgcta ccggatgcgc caaggctatt gtagaactga ttgtctttgt tgcggaagta
    81601 tgttggcaag attgaaacaa ccaatcccat taaataataa atcaggaata gtatggaacc
    81661 actttcaccg tttatcagtg taaaatgatt ttcaactata ggaccgaaac tgtatagcac
    81721 aaacatattc agaaagagat gcaaaaaatc tgcatggaca aaccctgatg taataaatcg
    81781 gtaccattgt ttgtgttgag ataccatata aggattaaaa atcattttag acaacaactc
    81841 attgtttcta aaacatatca aactcaataa aacgttggca atgatgatgt aaagtgtcat
    81901 gaaatctgta acgccataaa attacagaat ataacaattc
//
LOCUS       DAOOOI010000004        81525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_43_length_81525_cov_41.539573, whole genome shotgun sequence.
ACCESSION   DAOOOI010000004 DAOOOI010000000
VERSION     DAOOOI010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 81525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 81525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81525
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43_length_81525_cov_41.539573"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..868
                     /locus_tag="PKX59_01485"
                     /pseudo
     CDS             98..868
                     /locus_tag="PKX59_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660089.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
     gene            943..1530
                     /locus_tag="PKX59_01490"
     CDS             943..1530
                     /locus_tag="PKX59_01490"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOZ89567.1"
                     /translation="MKRCFDIIVSLIAIIILLPILLLIALWIKIDSRGSIIYKQIRVG
                     KGGRDFGIFKFRTMHPDSDIKGLLTVGGRDPRITNAGYLLRKYKLDELPQLFNVLKGD
                     MSLVGPRPEVRKYVSRYSDEQLKVLSVKPGITDFASIHYSNENEILAKSANPEQTYVQ
                     EIMPHKLALNLKYIQEASLMTDLKIIAATVMKIVS"
     gene            1726..4398
                     /locus_tag="PKX59_01495"
     CDS             1726..4398
                     /locus_tag="PKX59_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ89568.1"
                     /translation="MTISSFIAAIELNKEEKMRKFLNYYKSLFFVLLSLPSINTAQAQ
                     MQQMMQRGMNAGRLYGKVVDSKTNKPVEFAAVQISVFKKDSTGTLKENILNGQLTQGN
                     GDFSLDQLPVMGELTFKIAAMGYKPYEQKVKFEIKMPQGGGQGNNWQQALNAIDKDLG
                     NIKLEPNVVALQEVQIDGTAPVLELRPDKKVFNVEKNPIAAGGTAEDVLKQVPSVNVD
                     IDGNVTLRNAAPQIFVDGRPTTLTVDQIPADAIQEVEIITNPSAKYDASGGMAGILNI
                     VLKKNRRIGYNGMVRAGADSRGRINLGGDFNVREGKVNVFLSGNLNQRKSKGTGLTER
                     QTLGSTPNNFISQSNENSSNGYFGFIRSGADIFLDNRNTITVSGSYMNGYFKPLEFLQ
                     TKTDTLFPGYTSSSLSDRNSSSKREFKNTGGTVAYKRLFPQEGKELTADINFNSVKSD
                     MGGNYSTQNYLGTGEAYGNPILQKQSIEGENTFVTAQTDMDFPLDKKSKIETGLRGAM
                     RDYSSVNQNFIFDDSQQQYILIPNQTANYQYVDQVYAAYATYSKSFEKLSLQAGLRAE
                     SSFYEGELTQTGQKFSTNYPVSLFPSASASYKLSSKTDLQINYSRRINRPNFFQIIPY
                     SDYSDSLNISKGNPGLKPEFTHSVELTAMQTMSRKNTLMATVYYKQTDGVITRYLSYE
                     TDTILGRNVLVSTFRNANTSYAYGLELTAQNNIKGWLDITSNINAYQSVINGKNIESN
                     LKVEQFSWFAKLNLNVKLPKNFSVSISGDYQSKTALQLNSNSGGRGPMGGGTQSTAQG
                     YIRPNYGVDAGLRYDFLKDKAASLTLNVSDIFKTRVNDVHSESDFYIQDSWRKRDAQI
                     FRLNFSYRFGKFDTSLFKRKNQKINTEGMQDMSM"
     gene            4463..5680
                     /locus_tag="PKX59_01500"
     CDS             4463..5680
                     /locus_tag="PKX59_01500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ89569.1"
                     /translation="MNVLYISYDGMTDNLGQSQVIPYLKGLTGYGHHFDIISCEKPDK
                     FKAKEIEIRKLLDSLNIGWYPLPYTKRPPVLSTVIDVVRMYRKAYSLHKENKYDAVHC
                     RSYLSAMIGQDMKKKFGTKFIFDMRGFWPDERIDGDLWNIKNPVYRIIYNYFKKKELQ
                     FFNESDLNVSLTHAGLNEIKKMNVSNEQINKFSIIPCCSDYELFKPLPRDLALVKQLG
                     FEASNKILCYLGSIGTWYMFDEMMAMFKQLHFQDNSFRLFIMTPEPPSMVFDSAKKLG
                     INTDLIKVVSATRQQVPKYLSIAHLGISFIKPCFSKLSSSPTKMGEMLAAGVPLICNS
                     GVGDVERIINETKTGVIIHGFTEDEMQSVAKRAIEMIQVPRENIREQSRPVFALESGV
                     KLYAQAYEKMQKQ"
     gene            5746..6834
                     /locus_tag="PKX59_01505"
     CDS             5746..6834
                     /locus_tag="PKX59_01505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ89570.1"
                     /translation="MSHNKKKILLICPYPEDTAPGQRLRYEQYLNLFRKEGYDIHVSS
                     FMTYRFNSIVYKPGNVPEKIFWTLFGYIKRFLQLFTLWRYDAVYIFLWVTPFGLPLFE
                     WLFVLLNPNMIYDIDDMVFLGHSSKANKFIKILKGTRKITFLMKKAKHIVVSTPSNEE
                     FARKYNNAITDISVAINTDVYLPANKYSNNDVITIGWSGSHSTSRYLFLLEEVLRKFA
                     GRSNIRILVIGDTTFKFNGFDCETIPWQSATEVKDLQKIDIGLYPLPDEQWVYGKSGG
                     KALQYMALGIPTIATGIGTNFRIIDDGESGFLVRTDEQWIEKIEYLINHPEERKRIGM
                     NGRMKVEKEYSLHVNKKYYLEIFEKVVG"
     gene            6876..7784
                     /locus_tag="PKX59_01510"
     CDS             6876..7784
                     /locus_tag="PKX59_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89571.1"
                     /translation="MKKIIFVLLIACSHFVAKAQFAPVGAQWHYTQCTVTFPYSCGPV
                     EVNATGTTVINGITCTNLQIAPFTCVGFNYLPVYQSNDTVYRFLYNLNTFTMLYDFNA
                     QPGDIWNIVSNTINQPPPAPLTDTVRIRVDSVNMVNINNHPRKIFYISYADSSHIPFV
                     FEGPIIEGIGSLNFLLTQFNLCDPQITAFRCYNDSTLGLYQPNTLVDCDSVVVVGESE
                     VIKKDYVSLYPNPFTDQICIHSEDFLYRKYDIKLVNIVGKVVYHSKQRQALDQLTIYP
                     KNLAPGTYYLLLESDKKVLQKRVVKL"
     gene            7857..9863
                     /gene="ispG"
                     /locus_tag="PKX59_01515"
     CDS             7857..9863
                     /gene="ispG"
                     /locus_tag="PKX59_01515"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOZ89572.1"
                     /translation="MDSALTDQNIFQLYCNSLTSYQRLKTLEVKIGDVAMGANHPIRV
                     QSMTTTDTLDTKATVEQSIRMIVAGCEFVRITAPSINEARNLQNIKDELRKRGYKTPL
                     IADIHFTPNAAETAARIVEKVRVNPGNYADKKKFDTIEYTDVTYAEELERIRERFIPL
                     VKICKEYGTAMRIGTNHGSLSDRIMSRYGDTPLGMVESALEFLRICMDEGYYSIVLSM
                     KASNPQVMVQAYRLLIAKMRAEFMDLNNMQNTLPALFPLHLGVTEAGEAEDGRIKSAS
                     GIGTLLEDGIGDTIRVSLTEDPELEIPVAQKLVSRYNNRTSHQTIKPINHHKLPYSPF
                     SYSKRLTKAVLNVGGNHVPVVIADFSGKQNISPASLFPVGYNYSQSLDKWHISDQACD
                     FIYVGNQHISFALPGTLGVIYNNKMWLQHSDDERTYPLFNVNELNDALKCTAAIKFIE
                     LNTEDFFKQQLQVDKLSDAVLVLKTSNDHAMPDLRNVFVDMMQQKNTLPVIIKRDYAK
                     ADLETFRLHCSTDAGALLLDGMGDGVWLCSELVSPADINQTAFGILQAARTRISKTEY
                     ISCPSCGRTLFDLQETTAKIRARTHHLKGIKIGIMGCIVNGPGEMADADYGYVGSGAG
                     KITLYKGKEVVRRNIDAALAVDALIELIRDNGDWIEPAVITPSV"
     gene            complement(9847..11340)
                     /locus_tag="PKX59_01520"
     CDS             complement(9847..11340)
                     /locus_tag="PKX59_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOZ89573.1"
                     /translation="MKLLTSEQFRMADAYTIKTEPISSVDLMERAAICCSDEIAKFTD
                     EKDHICILCGPGNNGGDGLAIARILADAGKNVSVLLTDNSNKLSPDAEANFLRLQKTS
                     VKIFYLTDANLPFKNTTFWVDAIFGTGLNKAVEKNIADLIHQVNSTRTPVISIDLPSG
                     LFADKHTPTNVAVIHASTTLTFQRLKRSMLYAENAHRIGEVKVLDIGLDEHYMESLPT
                     TQELVDRSLINKILKPRKRFSHKGNFGHALLIGGSYGKAGAIILSAKACVKSGAGLTT
                     VHTVKTTGATLQSAIPEAMLSTCEGDKVCDLPDLAPYNAIAAGPGLGINEQTSSMLKL
                     LLQKAKKTLVLDADALNIISQNKNLIDNLPENTILTPHKKEFERLFGTFENDFQRNDF
                     QIAASVKHKIIIVLKGAYTSISTPEGKCFFNTTGNAGLAKGGSGDTLTGIITALLAQG
                     LNATDAAIAAVYIHGLAADCAVKKIHPYSLTASDVTEFLSEAFIQTV"
     gene            11458..12558
                     /gene="gcvT"
                     /locus_tag="PKX59_01525"
     CDS             11458..12558
                     /gene="gcvT"
                     /locus_tag="PKX59_01525"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOZ89574.1"
                     /translation="MEENTTLKHTALTAIHQKAGAKMVPFAGYLMPVQYTGINDEHET
                     VRNAVGMFDVSHMGEFILKGDDALDLLQRVTSNDASKLSAGKAQYSYFPNETGGIIDD
                     LIVYCIDEKTFMLVVNASNIEKDWNWLQKHNNKKVEMHDISDKTSLLAVQGPKAMETL
                     QKLTSIALADIPYYNFAKGEFAGCQNVLISNTGYTGAGGFELYFENQYAEKIWNDVMQ
                     AGQPFDIKPAGLGSRDTLRLEMGFCLYGNDIDDNTSPLEAGLGWITKFTKNFTNSDAL
                     KQQKEQGIANKLVGFEMIDRGIPRHDYEICNASGQKIGRVTSGTQSPTLNKAIGMGYV
                     SKDQSAEGTEIFIKIREKLIKAAVVKIPFIKK"
     gene            12562..13296
                     /locus_tag="PKX59_01530"
     CDS             12562..13296
                     /locus_tag="PKX59_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOZ89575.1"
                     /translation="MSEAGKPIVEVCLTPALFYQYNTKESIVVAIDILRATSSICVAL
                     QYGARSIVPVMTVEESFQYKEKGYLIGAERQGSMVEGFDLGNSPFSYMEDKIIGRDIA
                     LTTTNGTKAINIANGSAGIVAGSFLNLDVLAAWLIEQNKDVLCLCAGWKNTFNLEDTL
                     FAGALSQKLLPHFQLSEHRDSAIAAMNLYNLAKHDMYDFLKDSSHRTRLERLKIDEDV
                     LYCLTPNQTLVVPVLKDGVLKKHSDW"
     gene            13409..13747
                     /locus_tag="PKX59_01535"
     CDS             13409..13747
                     /locus_tag="PKX59_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89576.1"
                     /translation="MKKALSVLSVLGLFLIVNAYANSNKANTVTDLQVTESSIVANHD
                     MPCDGKAEKDKDCCKKKSKKKKCCADKAEASAASCADKQAKAGCCAHKKAGAGASDVK
                     TDTKSDESAK"
     gene            complement(13875..15332)
                     /locus_tag="PKX59_01540"
     CDS             complement(13875..15332)
                     /locus_tag="PKX59_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89577.1"
                     /translation="MKTKSKIIFFLLLFCSVSAFGQNLIWNPTVKLNDRPYPKFLGVN
                     NGYFYAAGSNIKMIQGASDSIFLATAWKKLKLTLLRYDDKFILKDTAAIRFLQFPVRV
                     YDVVLSDSTIKIFYSKGVGNLEFCADVFDLKGYFKQTVVLIAGKDVAKYNQKNFFSYN
                     TSINNRFFTITTNDSIYCFSNQLVSAWKTSLPNSTFFDGAVNDSGTFSGIFQRDKEYF
                     IATSVEGKIITQTISAEKEELSDFKIIHEGNRIIVVSLFGFTDNTFNPIYNDIANANK
                     FKSNGISISEFDNNLQLIKTNSIKFSEETLLETVDNLLLKNIKGVDFLKIQQMDVMSN
                     GNIMLLIEKQFASYTKPADVNGKKVASIGRETSANELLLICTDMQDKNMQTVIKRKVS
                     ATENFEYVCNVKGIPHKDDYYLYFLDGRNPEEYQMTQSIYNSQLRRIYTKEMDLASAK
                     KLLPDMSSITRLDKNRFILYARLLKKFSTAVLDFK"
     gene            complement(15316..16932)
                     /locus_tag="PKX59_01545"
     CDS             complement(15316..16932)
                     /locus_tag="PKX59_01545"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOZ89578.1"
                     /translation="MLLLLLICIGCSQKPIADLILFNGKIYTVDSTFSTAQAMVVSNG
                     KIVETGTNALADKYQCQQKIDLMGKSVYPGFIDAHCHFYGYSTDLSKCPLFGTKSFDE
                     VIQKVKEYAATNKFSWILGRGWDQNDWSENEFPDKSVLDSLFPNTPVYLMRIDGHAVL
                     VNQKALDIAGITTSTKVQGGEIITKNNHLTGLLIDNAVDIVKAHIPEFTAQIKTEGLI
                     KGEQNCFAVGLTSVTDAGLRVSDIRLLDSLQKAGTLSMKIYAMAELTNESKKYWFEKG
                     KYKTERLEVRSFKLYADGALGSRGACLLQPYSDLPGHYGFILQDKDSIQLAAKEVVEH
                     GFQLNTHCIGDSANRFMLKVYAANLKTKNDLRWRIEHCQVVNHDDLKYFSDYSIIPSV
                     QPTHATSDMYWAEKRLGSERIHDAYSNNALLKETGMLALGSDFPVEDINPLYGFYAAV
                     VRKDQKGFPPEGFETENKISREEALRGMTIWAAYSMFEENEKGSLEKGKAADFVIMND
                     DLMTAPDSILFKLPIETTYSNGIKVYENKK"
     gene            complement(17113..17463)
                     /locus_tag="PKX59_01550"
     CDS             complement(17113..17463)
                     /locus_tag="PKX59_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89579.1"
                     /translation="MVAAFFFGAAFFLVVAFFFGATFFLVAAFFFGAAFFLVAAFFFG
                     AAFFLVAAFFFGAAFFLVAAFFFGAAFFLVAFFFATGFFLAAFLPLALRAGFAFFLAA
                     DFFDFDAFAIGKQD"
     gene            17534..17731
                     /locus_tag="PKX59_01555"
     CDS             17534..17731
                     /locus_tag="PKX59_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89580.1"
                     /translation="MPKEKPEDIQPVVTYQTEIIEIVRPVEMDMDDNDAKMDEKEMMD
                     DDVSFNDDLFSGSSDDDDDDM"
     gene            complement(18008..18667)
                     /gene="pdxH"
                     /locus_tag="PKX59_01560"
     CDS             complement(18008..18667)
                     /gene="pdxH"
                     /locus_tag="PKX59_01560"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOZ89581.1"
                     /translation="MTLIMDDIHKVLKNVRSDYEADTFGTQEADDNPIVQFEKWFSLA
                     AQANLPDVNAMNLATCTKNGRPSSRIVLLRDATEEGFTFFTNYNSRKGNQLIENPYAA
                     LNFFWPELAKQIRVEGKVEKVDTKVSDEYFLSRPLESRIGAWASEQSQIIESRLVLEK
                     KFLSLKESMGSEVMRPPHWGGFILKPDYFEFWQGRQSRLHDRVAYTLNNNQWSKATLS
                     P"
     gene            18859..19422
                     /locus_tag="PKX59_01565"
     CDS             18859..19422
                     /locus_tag="PKX59_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOZ89582.1"
                     /translation="MEDFNQHAATGSLLISEPLQSDSFFKRSVILLSEHDTEGTLGFI
                     LNKLTDVTINDAVEDFPHFNAPLFFGGPVETDTLFYLHTVGHLLKDSKEIVKGVFLGG
                     DYDELKFLINTKQISPNQIRFYAGYSGWKPNQLEDELFHNAWMLGKGNAEFTFYPEPE
                     VLWSQVLRSMKNKYSLLANFPDDPTLN"
     gene            complement(19476..20132)
                     /locus_tag="PKX59_01570"
     CDS             complement(19476..20132)
                     /locus_tag="PKX59_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ89583.1"
                     /translation="MSIFIENIHKAYNGLKVLQGISLEIKTNELVAIIGPSGAGKSTL
                     LHIIGTIDKPDSGKVLLNNTDALALSSKKLAAFRNKHIGFVFQFHHLLPEFTSAENVM
                     IPAMIAGESESSALKRSNALLIQLGLEQRMHHKPSQLSGGEQQRVAVARALINNPDFI
                     LADEPSGNLDSGNAEDLHKLFLKLRNDYKKTIVIVTHNNQLAEMADRTITIRDGLLAQ
                     "
     gene            20189..22264
                     /locus_tag="PKX59_01575"
     CDS             20189..22264
                     /locus_tag="PKX59_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOZ89584.1"
                     /translation="MEIILATPYWYILLCLLAGLGCAWWLYGGNSPFNETPWLRRTLF
                     FLRFLSFSIIAFLLLSPFIKSAIREVEKPAIAIAFDNSSSILLDSTHFSASALNQLAE
                     KLTSAFKNDYEAGRYSFGSKVDNEVTDNDFSEKSTNISEMFDYLKSLYGGRNLGAVIM
                     LSDGIYNKGSNPVYTAEKLKVPVYTIAVGDTTINRDVLISDVAYNKVAFLGNNFPVEV
                     GVTMRQASGTAVTLTIKEDSLTLFSRMISASGNNYFAKIPVILSANRKGIHKYKIRIS
                     EVDGEKNLLNNEADIYVDVSERKMKILLLAQSPHPDIAAIKSVLDKSNLYETKVIFQE
                     QADENFSNYNLVILHQLPSAKSTTLLQKIKTAEVPYFIIAGAQMNVAAFNNSGTGVTI
                     NQPVAKMNEVYAKVSDQFGLFSLSDDMQKVLSQLPPLFVPFGNYKVEKETNTLLAQRI
                     GSIQSSMPLWYFSTSGTMRTGVLLGEGLWRWQLNEYALSGNHDVTDELILKSVQYLTS
                     KEKKEPFMIQYKRSYTEGENIEFDATLYNAAGELINEPEVRLNIYNDEKKEFRYTFNR
                     SNNAYQLNAGMLPPGRYTFSADTKAGDKVYKETGEFSVVPLLLENFESTANHQLLNTI
                     SSNTGGIMVTPDKINLLIDAVQKNETVKPVSYYHKKLIELISLKSLLAICLLLIAMEW
                     FLRKRFGSY"
     gene            complement(22261..23160)
                     /gene="miaA"
                     /locus_tag="PKX59_01580"
     CDS             complement(22261..23160)
                     /gene="miaA"
                     /locus_tag="PKX59_01580"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOZ89585.1"
                     /translation="MPKDKKLIIIAGPTASGKTQIAIEVAKLHSTEIVSFDSRQIFRE
                     MTIGTAVPDAQQMNTVKHHFIHSHSIAEDMNASRYADEATNTINQLLEKNETVVLTGG
                     TGMYMHAALFGLDPTPNTDYQLRASLEKECAEKGLEYLAHQLKTLDEKSYHQIDIHNP
                     RRVIRALEIIYSSGKTRSSFIEDKNKISTYNFTLYVLNPDRQLLYDNINQRVDMMIEK
                     GLVDEAKLLLPYRHLTALHTVGYKELFEYFDRNITLDQAIEKIKQHTRNYAKRQLTWF
                     RQYDDAVWITPEEMKKKIEKKNL"
     gene            complement(23187..23702)
                     /locus_tag="PKX59_01585"
     CDS             complement(23187..23702)
                     /locus_tag="PKX59_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89586.1"
                     /translation="MIRKILATQALLLCSASFLFAQHTLVLKSGEKMTGTVQQIKDGN
                     ISFLFKGNPMNFKVSEASAIFFDAASETKADNSEMSADMRGVKYEMPGRTITKPPKIE
                     NLTMEKGVVVVEISIDKYGHVTKANPGAEGTTTTSSYLLTKAKQAAESVMFNNCPKCP
                     LDTKGTITLTF"
     gene            23849..24868
                     /locus_tag="PKX59_01590"
     CDS             23849..24868
                     /locus_tag="PKX59_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOZ89587.1"
                     /translation="MKLVTYQNQNKTALGILLSDKVYNLSELSQRRGKTVLPDNMRDF
                     LTGEQLMMDAAMELNQWLSDGNADADGLSVANVNLLAPVPNPTSCRDGYAFRQHVEAA
                     RRNRRVEMIPEFDQYPIFYFTNHNAIQGPGDIFCMPDHFEQLDFELEAAIVIGRKGIN
                     VKAKDADSYIAGYMIMNDMSARKLQMEEMKLNLGPAKGKDFSTVIGPYLVTPDELASY
                     KTIAKSGHTGNNYNLKMTCKVNDIQVSEGNVGSMDWTFAEIIERCAYGVNILPGDVIG
                     SGTVGTGCFLELNGTGLFNNPDYKPQWLKEGDKVEMEIAGLGVLTNTIRKIDSDFSIL
                     ALKKM"
     gene            complement(24926..25699)
                     /locus_tag="PKX59_01595"
                     /pseudo
     CDS             complement(24926..25699)
                     /locus_tag="PKX59_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745327.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(25792..26253)
                     /gene="tnpA"
                     /locus_tag="PKX59_01600"
     CDS             complement(25792..26253)
                     /gene="tnpA"
                     /locus_tag="PKX59_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOZ89588.1"
                     /translation="MMSTYSQIYIQIVFAVKGRESLILPQWEERLHKYITGIVQNKEQ
                     KMLAINGMSDHLHFLIGMKPSCCLSDLVREIKKSSNDFIKVEKLSKFKFSWQEGYGAF
                     SYSHSQLNDVIAYITNQKEHHRKKSFKEEYVEFLKKFNVDYNEKYLFEWME"
     gene            complement(26592..29957)
                     /locus_tag="PKX59_01605"
     CDS             complement(26592..29957)
                     /locus_tag="PKX59_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOZ89589.1"
                     /translation="MQESKIYTPKHKIRIVTAASLFDGHDAAINIMRRIIQSSGAEVI
                     HLGHDRSAQEVVECAIQEDANAIAMTSYQGGHMEYFKYMYDLLKERGCGHIKIFGGGG
                     GTILPEEISELQDYGIARIYHPDDGRAMGLQGMINDMLQKCDFATGVNLNGEIKELTK
                     KNSKAIARLISCAENNQSEFEKLYPEITSSLSNKTKNSPVLGITGTGGAGKSSIVDEL
                     VRRFLLEFKDKYIAIISIDPSKRKTGGALLGDRIRMNSIRNPRVYMRSLATRQSNLAL
                     SRYVQEALDIVKAAQFDLIILETSGIGQSDTEITEHSNVSLYVMTPEYGAATQLEKID
                     MLDFADLIALNKFDKRGALDALRDVKKQYKRNHKLFDIGDEKIPVFGTIASQFNDPGM
                     NQLYKSVMNTIAAKTGADLKSDYHTTEEMSEKVFIIPPHRTRYLSEISENNRHYDQWV
                     EEQCTLAQKLYSLYESMQIIKGAAAEELKKQYDILERSFDGNNKKLLEDFPAKTEEYS
                     KDYYEFKVRDKVLKIKTFDESLSHNKIPKVAFPKYKAWGDLLKWILQENVPGEFPFTA
                     GVFRFKREGEDPTRMFAGEGCPERTNKRFHYVSLGMPAKRLSTAFDSVTLYGNDPDHR
                     PDIYGKIGNSGVSVCCLDDAKKLYSGFNLCDPATSVSMTINGPASIIAAYFMNAAIDQ
                     QCEIYIRQNGLEKEVEKKIADEFSKNKSSQPKYNYEIPEGNNSLGLMLLGITGDKVLP
                     KDVYDKIKTDTLKQVRGTVQADILKEDQAQNTCIFSTEFSLRLMGDMQQYFIDKAIRN
                     FYSVSISGYHIAEAGANPISQLAFTLSNGFTYVEYYLSRGMDINSFAPNLSFFFSNGI
                     DPEYSVIGRVARRIWAKAMKLKYKANERSQMLKYHIQTSGRSLHAQEIDFNDIRTTLQ
                     ALYAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINRELGLAKNENPLQGSFII
                     EELTDLVEEAVLSEFDRITERGGVLGAMETMYQRGKIQEESLYYESLKHTGAYPIIGV
                     NTFLSSKGSPTILPKEVIRATEEEKQQQIATLKNLHARYASTAPEMLHDLQLTAIHNQ
                     NMFEKLMEVSKYCSLGQITNALFEVGGQYRRNM"
     gene            complement(30106..31494)
                     /gene="lpdA"
                     /locus_tag="PKX59_01610"
     CDS             complement(30106..31494)
                     /gene="lpdA"
                     /locus_tag="PKX59_01610"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOZ89590.1"
                     /translation="MNFDLIIIGSGPGGYVAAIRASQLGLKTAIIEKENLGGICLNWG
                     CIPTKALLKSASVFEYLNHASDYGITVEGGKADFNAVIKRSRGVADGMSKGVQFLMKK
                     NKIEVINGFGKVKPGKSVEVTATDGSKKTYTAAHIILATGARSRELPNLKQDGKNIIG
                     YREAMSLPEQPKSMVVVGSGAIGVEFAYFYNAMGTKVTVVEFMPNIVPVEDEEVSKQL
                     ERSLKKVGIEIMTNSSVESVTVSGGTCSVKIKSAKGESVITCEKVLSAVGITTNIENI
                     GLEECGIITDKGKVVVNDFYQTNIPGIYAIGDIVKGQALAHVASAEGIICVEKIAGHH
                     PQPLDYNNIPGCTYCTPEIASVGMTEKAAKDAGIGIKVGKFPFSASGKAKAGGQSDGF
                     VKLIFDEKYGELLGAHLIGANVTEMIAELVLARKLETTGHEIIKAVHPHPTMSEAIME
                     AAAAAYNEVIHL"
     gene            31620..32090
                     /locus_tag="PKX59_01615"
     CDS             31620..32090
                     /locus_tag="PKX59_01615"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-R-sulfoxide reductase"
                     /protein_id="HOZ89591.1"
                     /translation="MKIRIFNIALVTLMGMWQLSIAQNSRKMEYNKLTPQEEKVLLFK
                     VTDRPFTGDYYLKKDVGTYICRRCNAPLYRSADKFEAHCGWPSFDDEIKGSVKRIPDA
                     DGMRTEIVCSNCDGHLGHVFLGEGFTAKNTRHCVNTSSLKFIPAGTPLPEVIKK"
     gene            complement(32096..33526)
                     /locus_tag="PKX59_01620"
     CDS             complement(32096..33526)
                     /locus_tag="PKX59_01620"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOZ89592.1"
                     /translation="MKFSANVSILLLCMATVSQTWAQPQVNIIEYANGIYGKTSDLAT
                     NGDGRMYAATQLGYIYIINPGGVTDTIPFLDINSKVTPTSLSNTGEQGLLGLAFSPNY
                     TTDSSFYVYYTNKTGNGNSVLARYKRSADPNVADTVSAEILFALAQPYTNHNGGCLKF
                     GNDGYLYLALGDGGDVGDPGNRAQNMNMYFGKILRIDVLQPGAYTIPSTNPFFGVMNV
                     KNEIWASGFRNPWKFSFDRLTHDMWIGDVGQDIWEEIDFQQANSTGGENYGWRCYEGL
                     VTYNTSVGCIGNYISPVHVYSHASTGGCSVTGGFVYRGTAYPALYGYYFFADFCNGTI
                     YALDPNNGNAVTVAGTFPGRAFSTFGEDDNGELYIADLVNQKVYQVTDTTTSISEYGN
                     DVNNVDMSFNQNEQSLQMNLTSKSNNQATLYLYDLKGTLITAWKLSLKSGNNTFKLPI
                     NLTSGIYLSNINSVNASLNIKIPVIK"
     gene            complement(33693..36338)
                     /locus_tag="PKX59_01625"
     CDS             complement(33693..36338)
                     /locus_tag="PKX59_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOZ89593.1"
                     /translation="MKKIFLLLFICILTYSAGYSQSCFTINSILVDACGNPEGENEMV
                     RFTVGASPLQVSSLNVSWPSTNLNFLGICQNANTAQKVADLNATIQSCGYLLEPSNDI
                     LPQNSKVILVTSENFNTTFNSFANLTDTMYVIFQCAGNTQGHFRNYSATVTTPRILTM
                     NFGAVCSQTVQYFPNQLVNQFGQLDTAAQDGSSVGFDSLGNPTYYNNGCQAPIISLTA
                     TLQSSATTVCSGNSVTLTASNLTGNYSGYFWSGGNGTLSTPNNLVTTYQTNATFTGTD
                     TIQFSMLGQCNDTITSNIYLIVQNGQPASITGPSTVCTGDSITLTASSGTAYQWSNGA
                     TTQSITVLTPGTYTVTVTGTCGTPTATATITGGTAPNITVTPGTNVSICAGQSVTLAA
                     SGASSYQWSTGATTSSISVNTAGTYTVSSTNACGTSSVTINVTQTTTPVVNITSNSGT
                     EICPGQSLTLTASGASTYVWSGGQTTPSITINTAGTYTVTGTSSCGTASNTITITAGT
                     APSISVSPGTSVNICSGQSATLTASGVATYLWSTGASTASINVSTAGNYTVTSSNACG
                     ADTVVVNVTVTNTPSVSVTSSATVICPGQSATLTASGASSYSWSGGQTGSSITVSTAG
                     IYTVTGTSTCGTASATYNLAQGSVPTAVISSPSTVLCNNSMLTLTATGGDNYLWSNGT
                     TTSTTTVNTPGIYAVTATNSCGSDTANFTITATNIDAAFAANVYSGQAPLTVDFTNNS
                     LNYTASSWAFGDGNTSTNNNASNIFNYGGTFNVVLTVTDNAGCTDTASAIIEVIDDSL
                     IIPNVITPNNDGKNDVFTYSSTAIKSAETTIFNRWGKKVTSYSDWKNGWDAKDNHAGV
                     YYYEIEIQLHNGTSKKYHGAINVIK"
     gene            complement(36436..36894)
                     /locus_tag="PKX59_01630"
     CDS             complement(36436..36894)
                     /locus_tag="PKX59_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89594.1"
                     /translation="MKKFYFLILSIFVVTSSCKEKNKLSDLDWIIGKWEGTARNGNIF
                     YEEWKKQSDTAFINNNYHFEGEKQVFGGTSKIVLRNNHLYYINDSDSLKILQWEAEGH
                     NSTSMTFYNEKITPYNRITFSLSEQNTWDAKLMGKKDTVKYSLKRKIMPY"
     gene            37112..38470
                     /locus_tag="PKX59_01635"
     CDS             37112..38470
                     /locus_tag="PKX59_01635"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOZ89595.1"
                     /translation="MNGIAHSNFAGNMGIEMNPASIATMPYRFEFNILSGDIFFQNDY
                     IYFPASDIPTGKFARFAPLRHEDYSDHYDKTPKNAYGNLLLKGPSAVWRNEEAAFGIH
                     FSLRSQLSAIKVPYHLAKFLWEGSDYSPQHKIPWNSTAFSAGALVFAEAGFTFAKTIL
                     TNRQNITTAGGTVNFLLGMSGTYINSTVLDYYFTDASLLNIKNIEVDYGHSIPNDDKN
                     NYLHPRGAGASMSFGVQHFFNYISQAYKPGMSGIKLKKYDLKLGLSLIDLGFIRFNGN
                     ARTFNIRNASAVWPGFDTTKITGYEHADSIFSAKLYGDANLSRSGSGFNMILPAAASF
                     QADWSITPKYYLNLAVIQNIQIFDRSVTRASQVSLTPRFETRRVEVSLPLSLYQYNFF
                     RAGIAVRYKWFVLGSDNLGWWPGLYEMNGLDFYFGFKYTSDIYDNIRFKKGKGGHCPA
                     YK"
     gene            complement(38700..39422)
                     /locus_tag="PKX59_01640"
     CDS             complement(38700..39422)
                     /locus_tag="PKX59_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89596.1"
                     /translation="MKKLIIPILCIAILAFTSCKKKDKDNPHYTCSSCVTQAEALAAN
                     DGSSKGVYKGIIIGSSGTIKFSIQNGTNDITATMVVDGVSATLTSTVSWVSGQPYIAP
                     FTGTLNGQAVSITFSVQNNGSTPQIISSNIPGHPNAVFTIIKETSNALVEGFEGTYST
                     TEPESGTFNILLSRPLGQWGGISRENGQTSTNDVSGTITSSGDLYDDNNIKVGTLSGD
                     EIDGTFTDNSGNTVTVDGQRTL"
     gene            complement(39456..41078)
                     /locus_tag="PKX59_01645"
     CDS             complement(39456..41078)
                     /locus_tag="PKX59_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89597.1"
                     /translation="MKQMLPKVTAVWLASAILSPTLLIAQGNETTHELSKPAQKGILA
                     NAELSADGNIRMTYKMKVDKKSDQVNYEDYVFDKNLKFIGVEKTKEDKQTQPDQKVTL
                     IKAFVGGTNSFNVMSMSLNLQQEVWERNWNFDKQKYQWGSRISKEVVKPKNNDSKYRG
                     FAAYGNDDDGSVLILASYDSGEDDQFVCLHVDKGLNMTETKLPLQGKYSLVYCGTLKS
                     GNIFAVFAPNEGMSDTKKYYYIECSADSKVAKSSEFTSPSPNMLIMDYSEANGDLYFI
                     AGSDKSTEAYNKVFTSYAPIDNPGYSTSNNKQMDKYEKRAMGTEFANLHLLRFSDGKL
                     AFASTEPVKDFKTKMVTPPSQKKGQPYTGKKIVIENLFVAPSGDVLVAGQLSDKKVVN
                     GGNSYEFRYYDYVCVQFDNQGKLVAQYAVNKMLDDSKNEVLSAIQNFVPSADGKSVYW
                     EILEVQAAKYYASVAAAYSGNYSLSSHYFPRIANITLSNHSVSNFTELGEKGKYLVYH
                     SITSLTDKDNNRFYIGHDEDYKNIWLGKYSFR"
     gene            complement(41146..41769)
                     /locus_tag="PKX59_01650"
     CDS             complement(41146..41769)
                     /locus_tag="PKX59_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ89598.1"
                     /translation="MTIQVGIIEDEKHIRENLQILIDGSEGFSCKHLFANAEDAINII
                     PTLQLDVVLTDIQLGGKTGIDCILELKDKCPSTNFLICTSFDDSEHVFKALKTGAMGY
                     LTKTTQPSKLLDSIVDVYRGGSPMNSHIARKVVSSFMETPVSPNYEKLSERERLIVDL
                     LSKGMRYKEIADQLFLSTETVRTHIRNIYHKLQVNSRTEALNKVFKK"
     gene            complement(41823..43844)
                     /locus_tag="PKX59_01655"
     CDS             complement(41823..43844)
                     /locus_tag="PKX59_01655"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOZ89599.1"
                     /translation="MSRFLSRIIFCVCLFFSHCVLYASDTTPTAQQKQIDTRISEAEE
                     IYKRDFKHAYVITLEAKSHAVKINYLQGIVASSCLQSIIESQTNKSIPAIAALTNLLQ
                     SSNGINLKLKQQLLSTLGICYFYIGKLDSAQFYFTESISSYSAHDTSIQLFNSCLQLS
                     EVFIKEGIPSKALDLYGKAYKIYQQNTSKEGEAWLNLVLGKIYYRQNLYEQSISTINT
                     TISQFASLNSLTGQISSLIQNGNSYYKQMLDDSAANCYQQALMYATTLGDERNKSICY
                     SNLSRIYLEHNKTKQALETALHALEVIHPGNYPEIEAGTYQQLGDIYVELGQKSKAIE
                     WIQKALLLAKDNNNKTIERDCYKSLSELYASIKQYEPAMENLLQAYRIKDSIQPIAFN
                     NRLAEMEARYSLEKKESTIKLLNQQSLINELRIKEQKENIRKQNALVIILFLLIATII
                     IALYFYLRWRKQAELINQQNTIRQTEENERMRIAKDIHDELGSGLTKIKFLSEILEMK
                     TKDQEQLKETLQTITGTSHQLIDNMRDMIWNMNPMNSTLDNLIARVREFSTEYLEDFP
                     IEVKYDLPENIPSEKISIEISRNILMIVKESLQNIVKHSNASKVSIRIEIQPVFKLII
                     TENGIGFNLNEKTTGNGLRNITERSKAIGASLKISSKPGEGSILSLEKI"
     gene            complement(43939..46593)
                     /gene="cphA"
                     /locus_tag="PKX59_01660"
     CDS             complement(43939..46593)
                     /gene="cphA"
                     /locus_tag="PKX59_01660"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOZ89600.1"
                     /translation="MRILEIRPMRGPNFWSIRRHKLIVMKLDLQEMEERPTDKIDGFL
                     ERLKSMFPSMYSHRCSEDHEGGFFKRVEEGTWMGHVIEHIALELQTLAGMDTGFGRTR
                     GTGEHGVYHVVFSYIEEKAGVYAARASVRIAQALIDGTGYDLQNDIQQLREIREDERL
                     GPSTGSIVEEAASRGIPWIRLNRHSLVQLGYGINQRRIQATIASTTSSIAVEIACDKE
                     DTKNLLEAQSIPVPRGKIIRDEDGLKDAVEDLGYPLVIKPLNGNHGRGATINIVNWDQ
                     AVEALEIAKKISRSVIVEKYITGYDHRILVINYKFICAAQRKPAAVVGDGKSTIQQLV
                     DAVNADPRRGYGHENVLTSIKIDEMTQNILEEKELTLQSVLKKGEELFLKRTANLSTG
                     GTATDVTDIVHPYNVFMCERIARIIGLDICGIDIMTPDISEPLTETGGAVLEVNAAPG
                     FRMHIAPTDGLPRNVAEPVVDMLYPPGSNYRIPIIAVTGTNGKTTTTRLIAHIAKTCG
                     HKVGFTTTDGIYIQNQMLQRGDCTGPQSAEFVLKDPTVDFAVLETARGGILRAGLGFH
                     RCDIAVVTNVAADHLGLKGINTLEEMSKVKAVVPESVQPNGYAILNADDELVSNMARN
                     LDCKVAYFSLDENNPLIKRHCEDGGLAAILENGFVTICKGTWKLRVHKVINIPLTFSG
                     KAVFMIQNILPAILSAFIRNFKIEDIRLALETFIPSPVQTPGRMNMFQFKKFNVMVDY
                     AHNPAGFQAIARFLEKVDAKPKVGVIAGVGDRRDEDIIALGTLAAKMFDDIIIRQDRN
                     LRGRTEQEIIDFMLDGIKAVDPTKPVKVIPLEKEAIDYAIKNGKKGSFIVICSDVIPD
                     ALDQIMKFKEKEDQFEMHPDDIPNQSNR"
     gene            complement(46809..47630)
                     /locus_tag="PKX59_01665"
     CDS             complement(46809..47630)
                     /locus_tag="PKX59_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89601.1"
                     /translation="MNNRLNKILIAAIPVLLIIGSCKKESTDIDYGVNEYQKDMINKA
                     MVVGSGGKLYEGPMPTATGGTIAITDKPNAVQVSAGVLLYFNYNTSNNANLCTFYVQI
                     DSADSYWAAPIEFDTIAQRPYVRILIPNFVRNGDYKLKFAVGDCAGNVSTIANTDVSV
                     TDPLGCGASFSGNVGITALVANLGSKAGTATISYDMYSIPDRLDIRYDGQWIASTGTL
                     LSPSGYPNCAISPDGFVSGSHSVTFNYDPGKSRLVEIYVSGCNSGTAWQIEVVCP"
     gene            complement(47623..48393)
                     /locus_tag="PKX59_01670"
     CDS             complement(47623..48393)
                     /locus_tag="PKX59_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOZ89602.1"
                     /translation="MKKKTKLMLAILICPIAIHAQIRLGQFKPSYNAEVFLGGSASFS
                     YGKFINEHKEFHEVSFSDADISGKIKPIVFFDAGGQFRVVLGEEDLLSFFSVSMGAFY
                     SKRGFTHLYSYSSDNSVQNINDEMSYTEKYRINQISIPILIRYGKKWFVEIGPSFDHF
                     ISATRKQILKREISGSGAYDGGFSTTETNQDNLNKNMFNEHKAGFILGLGTTLGDRFS
                     VRLTNHIFAKAYDKGSNYKSYNLQLEIMYNLFKLNNNE"
     gene            complement(48570..48845)
                     /locus_tag="PKX59_01675"
     CDS             complement(48570..48845)
                     /locus_tag="PKX59_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89603.1"
                     /translation="MLFTLGSCKKSTVDADLDYMNNLVTQLENKSKTEGLKFDDYMVL
                     QNDLLQMQHESEILTDRSKFTPEQKKRAEEITNRLGTLILKNKNIQL"
     gene            complement(48903..49496)
                     /locus_tag="PKX59_01680"
     CDS             complement(48903..49496)
                     /locus_tag="PKX59_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89604.1"
                     /translation="MKSFNIFKLSAFAFLCFTIVSCSQQDSGKQSENNNVQESEVASQ
                     LFSGLKSKLNSDDKKQISTLTGLTYDAASKTFMTEGEGVEVQVVPVDINNDKTEDIFV
                     ITYSSFLYGNTGQGFSLFMKDESGKFKNTLSLPGIPELLYESSESMPSIRVGGPGFDF
                     PIYKWKSHSYKLVKNSGKVSKALSLEEAGQQYTNSIK"
     gene            complement(49509..50576)
                     /locus_tag="PKX59_01685"
     CDS             complement(49509..50576)
                     /locus_tag="PKX59_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89605.1"
                     /translation="MKKLISILYCAFVMFQGIKHANAQESLNNQSIVSMVSAKLDNTI
                     IINKIKSSTNSFDFSTDGIIGLKGQKVSDKIISEMMLVSKNMPSLNNEDVIKMHTNKV
                     GKDLILKKINLSKCNFNTSTESLIALKNAGVPDPVVKVMMNPSTNSSASNTVSGDMKD
                     HPINIPAPTKLTSPGIYYEQFSPKVDYIMVEPTTTNQTKKGGFGESMGNHYSGGISGI
                     STKVGLAGEHSSTTIEDKRPVFYFYLSIDGKDINEVKESTYDGVASPKEFVLVKATTS
                     KSGRQIQIARKSAFSQEEGFAKGVVDFKIEKVSNRLYKVYFENDVPAGEYAFYYNKSS
                     DQKKSLKLYDFSLRNNITVVK"
     gene            50717..52795
                     /locus_tag="PKX59_01690"
     CDS             50717..52795
                     /locus_tag="PKX59_01690"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ89606.1"
                     /translation="MKHLWAIIVFLSLASSHAMPVQLDSLFIKANNYRNSDTVRATIL
                     NDLAIELADRDYEMGLLYSDSSIKLCHDINSDIKLATAYYAKARNLSTAGEDSLAIIS
                     YQKSYLLNLKERRFSACGTMLFNMGISYYNMSNYSQALEKYEAALNIFKKVKSEKGIA
                     ASLNSLGIVAMNLSDYSKAMEYYFECLKMAKNSDDSVLMANAYSNIGIVYKNIDDLSK
                     ALQYQNLALQINLIREEPLLIAKCYNNIAIVYSEMNQHNKAIEFYQKALEINREIKNN
                     FGIASNYINIGTDLNHLNRFSEANHYMLLAEELFNKIGDQSSEAMAIKARGELILNAP
                     DSVLKSLNIPKTQKYTVSLELLKRASAMFAEIGDISNRSDICQDLSMIYEYQGDAKNS
                     LAYYKQHISLRDSVMLDNKKAEIERTELKFNFELKEKELNLINSKIASDLQLAEQKRK
                     YLMMGSVLILLITGGGFVSYKLRKEAQQKRKDAEFKLANLNAELKSLRLQMNPHFMFN
                     SLNSIADFIDKNESRIASNYTVKFAKLMRMILEYSEQQEITLEEELKLLELYLSLEAL
                     RINPNLSYVISVSPEIDISNILIPPMMVQPFVENSILHGIIPLAKPGVIKIDVSVSID
                     ELIVIVEDNGVGINSSMKSDNKKSMAMKITKDRIALFNEQHSAAASVNLINLNQGTRI
                     ELRLPVIHRF"
     gene            52807..53550
                     /locus_tag="PKX59_01695"
     CDS             52807..53550
                     /locus_tag="PKX59_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOZ89607.1"
                     /translation="MIKAVIVDDEQHCINRITTLLKDYENEVQLVATASTVKEGLNII
                     KKVQPDLLFLDVEIADKTGFDLLREIESPSFDVIFTTAHDKYAVMAFKFCAIDYLLKP
                     VDESDFAIAMKKILDSDDRQDMTRQLSELIKGAGLNKPRKIGVPTLNGIVFVLISDIR
                     LCRADGNYTEIFLNNNHKIVVSKTLKEFDDLLSDYQFFRVHNSNLVNLDYVKSYNKGK
                     GGFLILDDDTNVEVSSRRKDELLKKLSLI"
     gene            complement(53576..54730)
                     /locus_tag="PKX59_01700"
     CDS             complement(53576..54730)
                     /locus_tag="PKX59_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ89608.1"
                     /translation="MDKIRILVVEDESIVAKDIQQTLIRLGYDVPATASSAQNAYARL
                     EEHEPDLVFLDIKLKGDLDGIHIAEHIKQKYDIPVIFLTSFVDKNTLDRAKITEPYGY
                     LVKPFNESDLQTTVEIALYKFQKDLQVRENERRFANALANVEEAILVADTEFRITFLN
                     PKAESMTGFGNESAQGMSLFKLIKIEGDEYEIVNADNLGKLMKQDEVLSIKQSNITIV
                     RDYSNIKADFTCSPIRDEKNQLLGYAVVIGSSKTEKSVAQPVIKPTEEKLVEAPSNPL
                     ENQVIQNSFFVKRGAMLVKVFLENIYWLQAMDNYVIIQTKEDQFVIHATMKDIETKLP
                     PEKFMRVHRSYIIALDKISHMDEGAVVINDKTIPIGKSYKEVFMNRINFL"
     gene            complement(54742..58065)
                     /locus_tag="PKX59_01705"
     CDS             complement(54742..58065)
                     /locus_tag="PKX59_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOZ89609.1"
                     /translation="MLFSSVVQGQHYNFTTYTIANGMPSNQVNAVHQDKAGKLWIGTM
                     NGACWFDGTNFYRFEQDNPASFNPVKAFYEDNKGNLWMAITRGGIGVFNGIRKKFYTI
                     DNGLLSNNATAITQDKTGRYWIGTSEGLNSFDGTRFSSYTILKGLVSNEVTCLLTDSK
                     GNIWIGTTNGLSKYDGKQFTNFTINEGLTGNIIYQISELNDGSIWAGTNEGISIYQNG
                     KFGAFDINKGVSVKKVTAILEDYKKNKWFSTYGNGMCLIDHGHFFTLTKDEGLTDNNI
                     LCMKEDNEGNIWIGTPRGLCRYSGGRFITYTTENGFNDNRLLTAYTDHLNRIWFGIVN
                     GGLNYIHGNTVYTPPFENILARATIWSIAQDAAQNFWFGTSTGPALVSTDLSKISFPF
                     DLLNNKIIYAVMAHRNGSLWFGTDRGIYIYENNNLRVLNKLNGLQNDNIRYLFQDRQG
                     LVWVGTLQGMYFMHNETAVSLNDKLNLNKTPITSITQDIEGNIIFSTFDFGVYIFSKK
                     KGTTAIRHLEVGNGLFNNRILTVASDNNYLWMGTPLGIDRIDWNTYLKRDFTNIIHFD
                     KSNGFYGVETNAATYDKDGFLWFATVNGAVRYNPNSGYSKSTTPVVLLQSIKAYMREV
                     NWSEQGFSIDSITGLPVNPVFPYGYNNLNFHFTGIYFASPDALQYRWKLDGFDDYWVP
                     ATNLNIANYSNLPPGNYTFQVQATANGHDWSPSLTYDFKVKSPVWRTNFFYFIYFVAL
                     TLLILVILKLRTRSLRNAQLLLRQKINERTHQLRENNMQLAKLSIVASETDNAVMIFD
                     HNLNLEFVNEGYTRMHGYTMDEIIRKQGSKLTQLSYNENIKTIIKDCLEEHKSVIYET
                     KITHRNGQQLWTSSTLTPVFNDKDELSNIVVIDTDITLHKKMEEQIRESLDEKNILLK
                     EIHHRVKNNLQIIVSLFNLQSAYLQDKASHKILKEGQDRIRSMALIHDRFYQSDGTSR
                     IDFDDYIKRLCETIMQSQNANPQKIRLQIEAEKISLDIDTAVPCGLIINELVSNAIKH
                     AFADDGGTIWVTFKKVDDHYLLSIADNGKGLPDGKDFETADTLGIQLVNVLSDQIDGK
                     VTVENSNGLKVIIKFKPSY"
     gene            complement(58187..59179)
                     /locus_tag="PKX59_01710"
     CDS             complement(58187..59179)
                     /locus_tag="PKX59_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOZ89610.1"
                     /translation="MNEFVRAALSNEAVSEAAKLYNIKFSDLTLLRDNVNIIYRFSEG
                     DKKFILRLTHSSHQCFDTVNSEIYWINFLHDNKIPVSNPLPSVNNNLCEKVTTGDSYF
                     SVVKFTAAPGVELSEKNWNSNTIAQMGRITGMIHKCSKTYSPPDTIKKRTIWPEKSNK
                     MVETYLPKSEMKSWEYWTQLLNIVKQLPSTSDSFGVVHNDIHRGNFMECNGRLTLFDC
                     EDACYTWFVNDISCPLFFAVNYNGVNDFNRRGWARYFMDSFMRGYQKYNRFDLNQLNL
                     MPELLHLRGIFVYAYLFKAWDVTKLNHVQLNYFNRMKHIAENGFDWLKPEDYLL"
     gene            complement(59183..60367)
                     /locus_tag="PKX59_01715"
     CDS             complement(59183..60367)
                     /locus_tag="PKX59_01715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOZ89611.1"
                     /translation="MAVKTLQLFESYLPNAQNWVFRMLNNLSDTEVNVAAYRYYNEQF
                     VTRKINLLPMPEYVSPDLLEGRDHAKNIFSKIINRIAILRKNKHYFEYIGKWANENGV
                     ELIHTHFANMGWQYLELKKITGLPYFVSFYGFDYESLPFRFQEWKKRYQSLYEQADGF
                     ICEGAFGASILVNQGCDPAKVHVVHLGVETTKIPAVARTKNEGELHLLQLSNYSQKKG
                     HIYTLQAFIQALNECPNMTLTFVGNELENIKGKLEALVKQHNIEHKVRFIGFIEFTKL
                     YEFFGQYHVFIHPSCYSDTRDCEGGAPIVLLDAQCTGMPVISTTHCDIPEEVIHGKTG
                     LLTPEKDAEALAGSIRTFYNMNNNTYQQFATAARQHVIENFDIKPCALQLQQLYNTIV
                     NH"
     gene            60511..62301
                     /locus_tag="PKX59_01720"
     CDS             60511..62301
                     /locus_tag="PKX59_01720"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOZ89612.1"
                     /translation="MSNFVVSARKYRPSSFDTVVGQGHITTTLKNAIKSNHLAQAFLF
                     CGPRGVGKTTCARILAKTINCSNITADTEACNECESCVSFNNGQSLNIFELDAASNNS
                     VEDIRSLVDQVRFAPASGHYKIYIIDEVHMLSSGAFNAFLKTLEEPPPYAIFILATTE
                     KHKIIPTILSRCQIFDFNRIKVEDISKHLQQIAVKENIEADPEALHIIAQKAEGALRD
                     ALSIFDQMVTFSGNKVTYKDVINNLNVLDYEYYFRITDQILPGNISSVLLTFNELLNE
                     GFDGHHFIAGLSGHFRDLMVAKDEITLQLLEVTEVVREKYKVQSKAVELSVLLKLIDI
                     CNACDLSYKSVRNQRLHVEITLMKMCALSGKLTVNENAMAAKPVVVEKVVAEKTNVAT
                     PAAQAPKSEPAVVSVKQESIKPEVVKPETKINEPAKPEVKEAPTVTKAEEPIATISLS
                     GLNAKKTTPDKAEPTDVKSEKKNDRPIVVDELNRIWALYADLQDNNGNTNLSKALLRT
                     EPEVINETEIHVTVDNQVLRDNLLSLKSEFLEYFKKEMQNDFLTLEVRVEASEKVLDK
                     PYTAREKFKKMAEKNPELENFRKQFDLNFD"
     gene            62341..63225
                     /locus_tag="PKX59_01725"
     CDS             62341..63225
                     /locus_tag="PKX59_01725"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008610865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOZ89613.1"
                     /translation="MIIDPTQIKTGEFHALMLGAVAPRPIAFASTVDKDGKPNLSPFS
                     FYNAFGSKPPILVFSPARRVRDNTIKHTLQNVFETREVVIGAVNYNIVHQASLASCEF
                     PEDVNEFEKAGFTAVKAEKVKPFLIKESPVNFECKVLDVIETGKLGGAANLIICEILL
                     MHIQDNVLNEQGKIDPDKIDLVARMGGDFYCRASGSAVFTVPKPNMKLGVGFDNIPEH
                     IKKSEVLTGNDLAQLANQETIPSTEEVAHFNQSDEMKKFNEALANDPLHAQLAKHLAA
                     RKLIHAGKVKEAWMLLLA"
     gene            63587..64549
                     /locus_tag="PKX59_01730"
     CDS             63587..64549
                     /locus_tag="PKX59_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89614.1"
                     /translation="MKKLILLIAVCCIFTQTTIAQINLVPNPSFEDTVACPTFLTQID
                     RATGWMSFSYTPDYFNACASSNVPVSVSVPHNIWGDQTAHTGNAYAGFGAYSVGSLLS
                     IREYVCTQLIQPTVPLQKYYVSFYVSSSFAYTNGFNPGVACNNIGMKLTTHSYSPSTP
                     LIPDNLAQVVNHSIVNDTINWVKISGSFIADSAYDFLCIGNFFVDSLTNYVLIGANSG
                     DAYYYLDDVKLSTDSAFVNSIVETKDNGYIKIFPNPARDWIVVEGRNLKSIEVFDLCG
                     RLLIPKVLVSSFKTEVNISSFSSGIFLFKIYTSTGIYINKFSHF"
     gene            64560..65072
                     /locus_tag="PKX59_01735"
     CDS             64560..65072
                     /locus_tag="PKX59_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89615.1"
                     /translation="MKNKFKYIIVLSLILCNVSLIFAQSTSATFNVIIPNRPNIAGND
                     YVGWDNTVNVPLEIRHNANQSIIFYTTGIQRATILGNGRVGIGINNPLWQLDVADEIN
                     ISQPRNYYMIGGETVLHNAGTENIFTGVNCGKDILAGSASGNMNVFNGYSAGEFASGN
                     DNVFIGHSTG"
     gene            65118..66008
                     /locus_tag="PKX59_01740"
     CDS             65118..66008
                     /locus_tag="PKX59_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89616.1"
                     /translation="MDNLGDYNSIVGFTAGMYLTTGIENTFMGLRSGECNTTGSYNTF
                     YGSRAGIGQNPSTPIVGRNNSFFGYASGQAIKDGNDNIFAGSHSGNSNSSGSYNTFIG
                     GNAGYNNEKGTYNTYIGNNAGSPVSYNLHYASAIGTNATVMNDKVMILGDNDINVGIG
                     MSGVLGGPLTKLQILSKNVPQFRISYDYGNNIHTDFQTTASGDLKINPTGHFTGFDLS
                     VSPANVVDINSSSSAISGLRLRNLSTTSNSNGKVLSVDNSGDVILVRDNSVELIAMIK
                     QLQQQVNSLQQKLEATLNQH"
     gene            66265..66438
                     /locus_tag="PKX59_01745"
     CDS             66265..66438
                     /locus_tag="PKX59_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89617.1"
                     /translation="MLIFYNSDGSILKMAKIQQIGEGSLLVYGSNLSSGQYSYSLVVN
                     NTTIKTKQMVKVK"
     gene            complement(66506..68662)
                     /locus_tag="PKX59_01750"
     CDS             complement(66506..68662)
                     /locus_tag="PKX59_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ89618.1"
                     /translation="MIISHIKKIVFVFAALLVATASFAQQDDLVLADQLYSKYSFGKA
                     IPLYEKVLSKEYNHGTASKLADCYRKNNQYKEAEKWYEKITADTNATNNEHLQYAKVL
                     ISLDKKDKAEMHLEKYLAKNPNDKLVAEQLNALKNNTFGQQQGSWIVKRLLINSVNGD
                     MCAVPYEDGIVFASSREDGKSKTQDWTSKPYLSLWFSKGKENNFSTPVLLELADKSKY
                     NDGPVSFSSDGNEMYITRNYEENGKIVRDQNRIVRLKILSATKVNGKFDAFKEFKYNN
                     KTYSCAHPFISKDGNKLYFASDMPGGKGGMDIWVCNRVNGDWDKPVNLGAEVNTTENE
                     VFPYIDHEGNLFFSSNGRSGMGGLDIFKCEFENGKFKTAENIGSPLNSTFDDFAYVYD
                     KKNMCGYLTSNRDTKNENDDLYMFKKNCVPLDGLVYDKETNLPLANAKVVINEGGNKF
                     AEVQTDDKGKFKSCLSIGQNYDFIASKDEYKENKVSINGVNDEPQVIKIPLSKIPAFT
                     LEGHVYLEEDKSALPNHPVKLMNLKTNKETETLTDANGNYHFDLEPETNYKVMTFREN
                     CADNFADRSTIGLTKSTVLKADFGFFCQGDVIRVDNIYYDLAKWNIRPDAAKELDKLV
                     DIMKKYPKMQIELGSHTDCRASFKYNMDLSEKRAKSAVQYIVKKGVEASRMTYKGYGE
                     SKLINHCECEGTRVVPCTEEEHQQNRRTEFKVLNVK"
     gene            complement(68682..69671)
                     /locus_tag="PKX59_01755"
     CDS             complement(68682..69671)
                     /locus_tag="PKX59_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOZ89619.1"
                     /translation="MKEAINRIHNNKISIAMYSTIKKIWIMSVLLTSICSIKANAQYD
                     PLFTQYMFNEMFINPAYAGSRDQISATALYRNQWVGMDGAPKTQTVSLHGPVKNKKVG
                     LGISFMNESIGVEKKVGFFTNYAYRLQTSENGRLAFGLQAGLIHMKENYLDVVTHDPN
                     DIQFSQNVSKLVPNAGFGIYYNTKRFYAGFSIPRLIENYIDSVNGGDIKNKFTHQNFH
                     YFLTGGYVFDVSPEIKLKPMTMIKASDGATIQADVTLAAYFNEIFWIGLTYRTGAAAS
                     VFAQVNLTKQLRLGYSYDYTTNQLNKYNSGTHEITLGYDIPLKRTGVLSPRFF"
     gene            complement(69676..81438)
                     /locus_tag="PKX59_01760"
     CDS             complement(69676..81438)
                     /locus_tag="PKX59_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOZ89620.1"
                     /translation="MIINTQTTYGTFAGYSATDDDRLYVRINNPAQEKIYFGIGQRST
                     NSDWYVRIKDPNGNIIFGPQKLPTSNAQGFISYHSQAIAGPNVVNAAGYNGYVCNPTI
                     GLPGAYYIEFNKGSGTTVAGNTELSLGIFDVTVVNTSSNTKVTGRLFSKNWGFNTESY
                     SNPFYGSFFIYGQDSSVTKVDLNGIKPFKFRVSCNRFGTSNSGNATTDRRSKTGFSVP
                     VEYRLFLTDPDIIAYPNGSMSFLTGPPTLERCVRDSICINVGLIKTSDVTVVIDRNNN
                     NKFDAGTADRKLFFPQVPAGSSCLYWDGKDGLGNLIAVGNPVSVILSIESGLVNLPMY
                     DVENHETGFSMSVIRPGSAQFVDSLFYDDVLVGGLTNLGGCPSPCHTWTSSPANTDQN
                     TIGESNTINTWWFAHKLSSKTIINLPDYLVNSAGTDKNLCTKGGGQDSIAFNGTIAYS
                     QSSFTGAVQWTTSGTGQFSPSDTVKNGYYIPSAADINAGSVMLIMGPKNVCATVKDTI
                     IINLRKSPQVSLTGTNINCFGQQTGGITTSVSNSSAPYTYSWSNGASSQNLSNVGAGT
                     YTVTVTSSQNCSTTATATLTQPAAALSASASATGVGCFGGATGAINLTVNGGTSPYNY
                     TWSNGATTQNVTGLTAGNYTVTVTDSKGCTSTQSSIGITQPTAALSLTNSKVNVNCFG
                     GNNGSISVTASGGTTPYTYLWNTGATSSSVTGLTAGNYTVTVTDANSCTTSQTSINIS
                     QPAAALAATPTAVNVNCFGNATGAINLSVSGGTAPYSYSWSNGATSQNISNIAAGNYT
                     VTVTDSKGCTSSQNSIAVTQPSASLTSSASPVAVDCFGNSSGSINVTVNGGTSPYSYS
                     WNTGATSQNLSNIIAGVYSVTVTDANGCTSSQNSISVTQPSASLTTSATGTNVNCFGN
                     ATGAVSLTVNGGTGPYSYTWSNGATTQNISNIVAGNYSVTVTDANGCTSSQSAISITQ
                     PSAALNSSVASTQNINCFGNGTGSVNISVSGGTTPYSFNWSNGATTQNLTNLTSGTYS
                     VTITDAKGCTSTTNNITLTQPSAAISANIQSMQNVSCNGGGNGALNMTVAGGTAPYTY
                     LWSNGATTQNINGLSSGIYNVTITDSKGCELQATGTVSQPSNALSASISSSQNVLCFG
                     GNNAHINLSVSGGTSPYTFNWSNGATTQNISNITAGNYTVTVTDDNGCIYTINKNISQ
                     PSGALAASVSASAEVSCFGLSNGSINVGVAGGTSPFTYSWSTGATTQNLTGLIAGTYT
                     VTVTDANGCTTSLNKTVTQPSAALAATLESSEEVSCNGGTNGQININVNGGTSPYSYN
                     WSNGATTQNIQNLSAGSYTVTVTDANSCTTSMTKTIGQPAQALNASIAATQNVNCFGG
                     SNASISITVGGGTTPYSYSWSNGATTQNISGLAAGSYSVTVTDANGCTTNAIQTITQP
                     AQALSASVTASQNISCYNVPNGSMSVTASGGTTAYTYLWNNGATTASISGLAAGSYTV
                     TITDANGCTTTADKTITQPSAQLDATISASANVSCFGLSNGSVSINVNGGTNPYTYAW
                     SNGATTQNITGLSSGVYTVTVTDNNGCTTTETATVSQPAQALNASVSSSQNVNCFGGN
                     NAAIQLSVSGGTTPYSYNWSNGATTQNISGITSGTYTVTVTDINNCTTTLTKAITQPT
                     AALAASVATSQNVSCYGQNNGNINLTVSGGTSPYSYAWSNGATTQNNNDLTAGSYSVT
                     ITDANGCTTQVNAITISQPAAALATSINTTDVLCFGLSTGAISTTVSGGTAPYSYLWN
                     TGATTSGLSNVNSGTYSVTVTDAQGCTTSQAAIQVNQPAAILDANVATNVSVLCYGGN
                     NGSLTLAVSGGTAPYTYLWSNGATTQNITSITAGTYSVTITDANNCSKAITGVLVNQP
                     DNPLTTSVASSQNVACNGGGNGSITLNVAGGTLPYTYNWSNGATTQNISGLYAGTYNV
                     SITDANGCTAAATGVVNQPSGALTSSVASSQDILCYGGNNGSVTLSVVGGTAPYTYSW
                     SNSATTQNLSNLSAGNYAVTVTDANNCISQSSVVIAQPSGALAANISASQNILCYNQS
                     TGSIDVSVTGGTAPFTYNWSNGATSQDITNITAGTYSVSITDANGCTTQMNGIVLTQP
                     SGALAVTPQLTAVNCYDNYTGAITLNVTGGTAPYSYNWSNGATTQNISNIGAGTYSVT
                     VTDANGCTSASTSLQITQPTAALNNTFTAQNINCYNVNTGSISVNVTGGTTPYSYNWS
                     NGATSQNLSNLAAGTYTLTITDANGCTSSTGSVSLTQPAAALAYTFTTSDAGCFGNNT
                     GGIDLTVTGGTAPYSFSWSNGATTEDISGIASGTYVVTITDGNGCTNTSASITVSQPA
                     GALNSAVSSSQNINCFGNQTGSITLSVNGGTAPYTYNWSNGATTQNISNIGAGTYSVT
                     ITDANGCSNLLSGIALTQPTGALATSISGTQNVNCYGQSTGNITLNVNGGTAPYSYNW
                     SNGAITQNLSSLSAGTYSVTVTDANNCTSSINNIIISEPAAVLFGSVNSAVNVNCTGG
                     NNGSISLNVSGGTSPFSYNWSNGATTQNISGLTSGSYAVTITDANGCTFSLSEIITQP
                     STSLSSAITSSQNINCFGNATGSIDLSANGGTTPYNYNWSNGATSEDLNAIAAGTYTV
                     TITDANGCTSSQSATLTQPSATLTSSINNLTNVGCYGQSTAAILINVNGGTTPYSYLW
                     SDGSTSQNLIGVGAGNYTVNITDANGCTTSIAGIAISQPAAGLGATVTSNQNVSCTGG
                     GNGSIDITVNGGTMPYSFNWSNGSVSEDISNLGTGAYTVTITDANGCTYTLTENVGQP
                     PSNLATSIQSSQNVDCFSNATGAIDLNASGGTAPYTFVWSNGATTEDISNLASGIYTV
                     TVTDANGCYNNQTVTISQPAGALASSITGMQNILCFGNTTGNIDLTITGGTTPYNFNW
                     SNGANTEDLSNIAAGTYSVTVTDANNCTVQQNITLTQPAAGLTSSITSTSPVLCFGGN
                     TGSANLDIQGGTTPYSIVWSNGSTTQNQPSLAAGSYNVTITDANGCTSNQSVTINQPQ
                     DSISGGISSIGNIGCFGNASGNIDLTVNGGTTPYTYLWNSGQTTQDLNNITSGLYEVT
                     VTDQNGCTWSISANLSQPALALSNNLSLQQNVNCFGGNNGAINTNPVGGTSPYTYFWS
                     NGNNTQNISNLASGWYSVTVTDANGCSATDSIEVQQPAAAVNATYQMTSSVSCFGGNN
                     GAVDVTVTGGTMPYSYVWNTGDVTEDLSNLTAGNYTVSITDANGCTFSLVTAVTQPNG
                     PLSPSIVMTPVGCFADSTGAINLTVTGGTPPYQYYWSNGDTTANLTNLTGGSYSVYIV
                     DSNSCIAQTSTYVPTPEGALYAFPVVTNLDCSSLTSGSIMLNPTGGTVPYSYSWSNGD
                     TTENLVNIGAGQYTVTITDSAGCTFSQTFTINGPTQALSLTGQQTNINCHGNNTGSAS
                     VTASGGIAPYSYIWTNGMTTPSISNLTAGNYKVIVTDSYNCSTDTVFVITQPDSILSN
                     IISATQIPCYGLNNGSISVTTQGGTPAYTYQWNNGSSSSQLSNLTPGTYSLTVTDQKG
                     CTDQQTVTITQPAFALTASSTANEANCLTGNGANIDLTVAGGTQPYAYVWNNGSTYQS
                     LTNVGAGTYTVSITDANGCTVQQTQVVTDISVFNMNADGPTEFCVGGKVTLNATVVQN
                     GTYQWYVNGQVLPGATSPSLTTPAAGVYTVSLTNSCGNFTSTPVEVKVNSVSGYTVSP
                     GMMICPNNGEIAQLFATGGSWYSWHPGAGLNDSTIANPIATPKASTTYTVTIRSNEGC
                     EVVSEVMVSVICDTLFVPTGFSPDGNAVNDTYVIDGLSKYPGNLLYIYNRWGNLVYKK
                     RDYDNSWDGTSNVAGVYLGQQLPNGTYYFILDLNDGKKPMQGYLTLKR"
BASE COUNT        24556 a        14827 c        15508 g        26634 t
ORIGIN      
        1 ttttgaagta tattaaacct taaaatatgt gttcaccaat gtctaaaaaa tataattctt
       61 tgatttgtag catcccgttt ttttaatgtg gaaagatttg gtaaaatcac gcccggttgc
      121 caatcattat tatattctta aaagtaaggc agaaggctct tctgcattgg cgttttataa
      181 tcctataacc gatagaattg aacgtattca taaagatcat gcttatggta taaaacctcg
      241 taatgccgaa caggcttttg ccatgcatgc agtcatgaat ccgaatgttt tgttggtaac
      301 ggttcagggt gtggccggaa caggtaaaac tttaattgca ttagcatcgg caatgcaaca
      361 aaaaatgaat taccgtcaga tatatcttgc gcgtccgata gttccgctca gcaataaaga
      421 tattggttat ttgcccggag atataaagtc aaagattaat ccatacatgg agccattgtg
      481 ggataatctt aaatacattc aaagtcaata tcgcgaaaca gataaagagt atcagaaaat
      541 ttcagatatg gtagagaacg aaaaattgca catcacacca ttggcatata tacgtggccg
      601 tagcttaagt aatatttgtt ttattattga cgaagctcaa aatctgacac cacatgaagt
      661 taaaactatc attacccgtg ctggtgaagg aacaaaaata attttcacag gtgatgtgtt
      721 tcagattgat acaccatatc ttgatgcaca tagcaatgga ctaagcaatg taattgataa
      781 gatgaaaaat tctcatctct attcgcacat aactttggaa aaaggagaac gaagtgagtt
      841 ggcaaatctt gccggtgaat tactttaaac aagtaaccgg attttttaaa tataattttt
      901 attcaaggta ggtagagata cagaagttat ctttgcgaca tgattaaacg atgctttgat
      961 attattgtat cattaatagc aataattatt cttttgccaa tactattgct cattgcatta
     1021 tggattaaaa ttgatagcag aggtagtatt atttacaaac aaataagagt aggtaaggga
     1081 ggtagagatt ttggtatttt taaattcaga acgatgcacc ctgactctga cataaaggga
     1141 ttgctgactg tgggtggacg tgatccaaga ataaccaatg cagggtattt acttcgtaag
     1201 tataaacttg atgaattacc gcaattgttt aatgtgttga aaggcgatat gagtcttgtt
     1261 ggtccaagac cggaagtgcg caagtatgtt tcacgttatt ccgatgaaca actaaaggta
     1321 ttaagcgtaa aaccgggaat tactgatttt gcaagcattc attacagcaa tgagaatgaa
     1381 attttagcga agtcagcaaa ccctgaacag acctatgtac aggaaattat gccacataag
     1441 ttagcactca atctaaagta tattcaagaa gcaagcctaa tgacagacct gaagattatt
     1501 gccgctacag tcatgaaaat tgtcagttga ataataagtt tatttataga cctttaattt
     1561 gtttttaaaa ctgtgttaat gggtgttaat ttttctattg tttgagtgag agtaaatgat
     1621 tttaaaatga gttttcagtt gatgaaataa gttttaaatc cgcttaaact ttaaagagga
     1681 aaaaagtgtt attgttgcaa ttgaattgaa cagtaaagaa ttaaattgac tataagtagt
     1741 tttatagctg ccattgaatt aaataaagaa gagaagatgc gtaaattttt gaattattac
     1801 aaaagcttgt tttttgtttt gttgagtttg ccaagtataa acacagcgca agcacaaatg
     1861 cagcagatga tgcaacgcgg tatgaatgcc ggtcggctct atggaaaagt tgtagattcc
     1921 aaaaccaata agcctgttga atttgctgca gttcagattt ctgtttttaa aaaggacagt
     1981 acgggcacat taaaggaaaa tattctgaat ggacagttaa cgcagggcaa tggagatttt
     2041 agtttggatc agttgccggt aatgggtgaa ctgactttta aaattgctgc aatgggatat
     2101 aaaccttatg agcaaaaagt aaaatttgaa ataaaaatgc ctcaaggtgg tggtcaggga
     2161 aataattggc aacaggcact caatgctata gataaagatt taggtaacat aaagcttgaa
     2221 cctaatgtgg ttgcacttca ggaagttcag attgatggta cagctccggt gttggaactg
     2281 cgtcccgaca agaaagtttt taatgttgaa aaaaatccaa ttgcagcagg aggtacagca
     2341 gaagatgtgc taaagcaagt tcctagtgtg aatgttgata tagatggtaa tgtaaccttg
     2401 cggaatgccg caccgcaaat atttgtagat ggccgtccaa caactttaac tgtagatcag
     2461 attcctgctg atgccattca ggaagttgaa attattacaa atccatcagc aaagtacgat
     2521 gccagcggag ggatggccgg tatattaaac attgttttga aaaagaacag acgcattggc
     2581 tataacggaa tggttcgtgc cggtgcagat tcaagaggaa gaataaatct ggggggagat
     2641 tttaatgtgc gcgaaggaaa agtaaatgtg tttttaagcg gcaatctcaa tcagcgtaaa
     2701 tcaaaaggaa ctggactaac agaaagacaa acactgggct ccactccaaa taattttata
     2761 agccaaagta atgaaaattc cagcaatggc tattttggat ttattcgttc aggagcagat
     2821 atttttttag ataacagaaa taccataact gtttcgggaa gttatatgaa tggatatttt
     2881 aaaccattgg agtttttaca aacaaagacg gatacattat ttccgggata tacatcttct
     2941 tctttgtcag acagaaattc ttcaagcaaa cgtgaattta aaaatacagg tggcacagtt
     3001 gcctataaac gtcttttccc gcaagaaggt aaagaactta ctgcagatat aaatttcaac
     3061 agtgtaaaga gtgatatggg aggaaactac agtacacaaa attatctggg cactggcgaa
     3121 gcttacggca atcctatttt gcagaagcaa agtattgaag gtgaaaacac atttgttaca
     3181 gcacaaaccg atatggattt cccgcttgat aaaaagtcga aaattgaaac aggactgcgt
     3241 ggtgctatgc gcgattacag cagtgtaaat cagaatttta tttttgacga ctcgcaacaa
     3301 caatatattt taattcctaa tcagacagca aattatcaat atgtagatca ggtttatgca
     3361 gcttatgcaa cctacagtaa gtcattcgaa aaattatcat tacaggcagg actaagggca
     3421 gaaagctcgt tctatgaagg tgaacttaca caaacaggac agaagtttag cacaaattat
     3481 cctgttagtt tgttcccaag tgcatcggca agttataaac tgagtagtaa aactgatttg
     3541 cagataaatt attcacgcag aataaacaga cctaactttt tccagataat tccatattct
     3601 gattattccg attctttaaa catcagtaaa ggaaaccccg gattgaaacc tgagtttaca
     3661 cattcagtag aattgacagc catgcaaact atgagtcgta aaaatacact gatggcaaca
     3721 gtttattata aacaaactga tggggtaatt acccgttatc tgagctatga aacggatact
     3781 attttgggac gtaatgtatt ggtaagcacc ttccgaaatg caaacacaag ctatgcttat
     3841 gggttagaat taacagcaca aaataatatt aaaggttggc tcgacattac ttcaaatatt
     3901 aatgcttatc agagtgtgat taatggaaaa aacattgagt caaatctaaa agttgaacag
     3961 tttagttggt ttgcaaaact caaccttaat gttaaactgc ctaagaattt ttctgttagt
     4021 atttcaggtg attatcaatc caaaacagca ttacagttaa atagtaatag tggaggacgt
     4081 ggaccaatgg gtggcggcac tcaaagtacg gcacagggtt atataagacc aaattatggt
     4141 gttgatgctg gtttacgtta tgattttctt aaagataaag cagcatcact tacgctaaat
     4201 gtttctgata ttttcaagac aagagtaaat gatgtgcatt cagaatctga tttttatata
     4261 caggatagct ggcgcaaacg agacgcacaa attttcagat taaacttcag ctatcgcttt
     4321 gggaaattcg atacctcact attcaagagg aaaaatcaga aaataaatac tgaaggtatg
     4381 caggatatga gcatgtagtg tttattcttt cgttcgttaa tgcatctgaa ttcggttcaa
     4441 atgcaatatt tttggacgct ttatgaatgt actttatatt tcttatgatg gaatgacaga
     4501 taatcttggt cagtcgcagg taattcccta tctgaaagga ctcacaggtt atggtcatca
     4561 ttttgatatt atcagttgcg aaaagcccga taagtttaaa gctaaagaaa tagaaattag
     4621 aaagttgctt gattctttaa acataggatg gtatcctttg ccatatacaa aaagaccccc
     4681 tgtattatca acagtaattg atgtggttcg tatgtacaga aaagcataca gtctgcacaa
     4741 agaaaacaag tatgatgcag tgcattgtcg aagctatctc tctgctatga ttggtcagga
     4801 catgaaaaag aagtttggca ccaaatttat ttttgacatg cgaggcttct ggcccgatga
     4861 acgcattgac ggtgatttgt ggaatattaa aaatccagtt tacagaatca tttataatta
     4921 tttcaagaaa aaagaactgc agttttttaa tgagtcagac ttgaatgtca gtctcacaca
     4981 tgcagggttg aacgaaatta aaaaaatgaa tgtcagcaac gagcaaataa acaagttctc
     5041 tattattcca tgttgctcag attatgaatt gttcaaacct ttaccgcgtg atttggcatt
     5101 ggttaagcag ctaggttttg aagcatcaaa caagatattg tgttacctcg gttcaatagg
     5161 aacatggtat atgtttgatg agatgatggc tatgtttaag cagttgcatt ttcaggataa
     5221 ttccttccgc ctgtttatta tgacacctga acctccatca atggttttcg attcggcaaa
     5281 aaaattggga ataaatacag atttaatcaa ggtagtgagt gcaacgcgac agcaggtgcc
     5341 aaaatattta tcaattgcgc atctgggaat ttcatttatc aaaccttgct tttcaaaact
     5401 aagcagttcg ccaacaaaga tgggggagat gcttgctgca ggagttccat tgatttgcaa
     5461 cagtggtgtt ggcgatgttg agcgtatcat taatgaaaca aagacaggtg taataattca
     5521 tggttttaca gaagatgaaa tgcagtctgt agcaaaaaga gctattgaaa tgattcaggt
     5581 tccaagagag aatatcagag aacaatctcg tccggttttt gcacttgaaa gcggagttaa
     5641 actatatgca caggcttatg aaaaaatgca aaaacaataa ttacaaaatc atctttattt
     5701 tcgcagacaa tttgctgacc ttgtttttgc aggcaacaaa aatttatgag ccacaataag
     5761 aagaaaattc tactgatttg cccttatccc gaagatactg caccggggca acgtttgcgt
     5821 tatgagcagt atctgaattt gttcagaaaa gaaggatatg atatacatgt ttcatctttc
     5881 atgacctatc gttttaacag cattgtttat aaacccggaa atgttccaga gaaaatattc
     5941 tggactttgt ttggctacat aaaacgcttt ttgcaactgt ttacgttatg gcgttatgat
     6001 gcagtgtata tttttttgtg ggtaacacca ttcggattgc cgctgtttga atggcttttt
     6061 gtgctgctaa accctaatat gatttatgat attgatgaca tggtttttct tggtcattct
     6121 tctaaagcca ataagtttat taaaatatta aaaggtacaa gaaaaattac tttcctgatg
     6181 aagaaggcta agcacattgt tgtttcaaca ccttctaatg aggagtttgc cagaaagtat
     6241 aacaatgcaa ttactgatat ttcagtagct atcaacacag atgtttatct tcctgcaaat
     6301 aaatacagca acaatgatgt tataacaata ggatggagtg gcagtcacag tacaagccgt
     6361 tatttatttt tgttggaaga agtgttgagg aaatttgccg gtcgcagtaa tatcagaatc
     6421 ctggtaatag gagacacaac gtttaagttt aatggcttcg attgtgaaac cattccctgg
     6481 caatcggcaa cagaggttaa agaccttcag aaaattgata ttggcctata tccacttcct
     6541 gatgaacaat gggtttatgg gaagagtgga ggcaaggcat tgcaatatat ggctttggga
     6601 attccaacca ttgcaaccgg aatcgggaca aacttcagaa taattgatga tggcgagtca
     6661 gggtttctgg taagaacaga tgaacagtgg attgaaaaaa ttgaatatct gataaatcac
     6721 cctgaagaga gaaaacgaat tggcatgaat ggaagaatga aggttgagaa agagtattca
     6781 cttcatgtaa acaaaaaata ctatctcgaa atatttgaaa aggtagttgg ttgaatattt
     6841 gtttgtcact ttgttttaag tatcttagca ctgcaatgaa aaagataatt tttgtattgc
     6901 ttatagcttg tagtcatttt gtagctaaag cacagtttgc acctgttggt gcacagtggc
     6961 actacacgca atgtacagtt acatttcctt attcctgcgg ccctgttgaa gtaaatgcaa
     7021 ctggaactac tgtcattaat ggcattacat gcactaacct acagattgct ccttttactt
     7081 gtgttggctt taattatttg cctgtttatc agagtaacga tactgtttat cgttttctct
     7141 acaatctcaa cacctttacc atgctttacg attttaatgc acagccgggt gatatatgga
     7201 acatagtatc aaacaccatt aatcaaccgc caccggcacc acttacagac actgtgcgta
     7261 ttcgtgttga ctcagtgaac atggttaata tcaacaatca tccccgaaaa atattttaca
     7321 tttcttatgc cgattcatct cacattccat ttgtttttga agggcctatt attgaaggta
     7381 ttggcagttt gaatttttta cttacacaat tcaatttatg cgatcctcag ataactgctt
     7441 ttagatgtta caacgattca acattaggtt tgtatcagcc caacaccttg gttgactgcg
     7501 attctgtagt tgttgttggt gaaagtgaag taatcaaaaa agactatgta agcctttatc
     7561 cgaatccttt cacagatcag atatgcattc actctgaaga ttttttatac agaaagtacg
     7621 atattaaatt agtgaatatt gttggtaagg tcgtttatca cagcaagcaa aggcaggctc
     7681 ttgatcaact tacaatttat cctaaaaatc ttgcacccgg cacttattat ctgctgctgg
     7741 aatctgacaa aaaggtgctt caaaaacggg tggtaaaact ttaatgtctt tactcggtta
     7801 tgctaatcta aagaaataca attccgatat ttgcatacta aatatttatt ttaaaaatgg
     7861 atagcgccct cacagatcaa aatattttcc aactatattg taacagcctt acttcttacc
     7921 aaagactaaa aacacttgag gtaaagattg gcgatgtagc aatgggagct aatcatccca
     7981 ttcgtgttca aagtatgacc actacagaca ctcttgacac caaggctaca gttgaacaaa
     8041 gcatcaggat gattgtggca ggttgtgaat ttgtgcgaat cactgcacca agcatcaatg
     8101 aggcacgtaa tcttcagaat attaaagacg aacttcgcaa gagaggctat aaaacacctc
     8161 tgattgccga tatccatttt acacctaatg ccgcagaaac tgcagctcgt attgtggaga
     8221 aagtaagggt gaatcccggc aactatgctg acaagaaaaa gttcgacact attgaatata
     8281 ctgatgtaac ttatgctgaa gagttagagc gaatccgcga acggtttatt ccattagtaa
     8341 agatttgtaa agagtatggt actgcaatgc gcattggtac caatcatggt tctttgagcg
     8401 atcgtattat gagtcgttat ggcgatacac ctttgggaat ggtggagtct gctcttgagt
     8461 ttttgcgaat ttgtatggat gaaggttatt atagcatcgt actttctatg aaagcaagta
     8521 atcctcaggt aatggttcag gcatatagac ttttgattgc taaaatgcgt gcagagttta
     8581 tggatttgaa taatatgcaa aacactttgc ctgctctttt tcctttgcat cttggtgtaa
     8641 cagaggcagg cgaggctgaa gacggacgaa taaaatcagc atcaggtata ggcacattac
     8701 tcgaagatgg tattggcgat accataagag tttcgttaac cgaagatccg gaacttgaaa
     8761 ttcctgttgc acaaaaattg gtgtcacgtt ataataatcg cacatcacac caaacaataa
     8821 aacctattaa ccatcataaa ctaccttatt ctccattcag ttacagcaaa cgactaacaa
     8881 aagctgtttt gaatgttgga ggaaatcatg tcccggttgt gattgcagat ttttcaggca
     8941 agcaaaatat ttctcctgct tcattatttc ctgttggata taattattca cagtcattgg
     9001 ataaatggca tatcagcgat caggcatgtg attttattta tgtaggtaat caacatattt
     9061 catttgcatt gcccggaaca ttgggtgtca tttacaacaa taaaatgtgg ctacaacatt
     9121 ccgatgatga aagaacttat ccgttgttta atgtgaatga gctgaatgat gcattaaaat
     9181 gcactgccgc cataaagttt attgaattaa acactgaaga tttttttaaa caacagttgc
     9241 aagttgataa actttctgat gctgttcttg tcttaaaaac atctaacgat catgcaatgc
     9301 cggatttacg taatgtgttt gttgatatga tgcagcagaa aaatacttta cctgtcatca
     9361 taaagcgtga ttatgctaaa gctgatttag aaacatttcg tttacattgt tccactgatg
     9421 caggtgcatt gttattggat ggtatgggtg atggtgtttg gctttgttca gaattagtta
     9481 gtccggcaga tattaatcaa acagcatttg gaattttaca agctgcacga acacgcattt
     9541 caaaaacaga atatatcagt tgcccttcat gcggacgaac attattcgac ctgcaggaaa
     9601 ccactgctaa aatccgtgca cgaacacatc atctcaaagg cattaagata ggtattatgg
     9661 gatgtattgt aaatggtccc ggtgaaatgg ccgatgcaga ttatggctat gtaggttccg
     9721 gtgcaggaaa aataacactc tataaaggca aagaagtcgt ccgcagaaat attgatgccg
     9781 cacttgctgt tgatgcactc attgaattga ttcgcgataa tggcgactgg attgagcctg
     9841 ctgtaattac accgtctgta taaaagcttc agaaagaaat tctgttacat cacttgcggt
     9901 taaagagtaa gggtgaattt ttttaacagc acaatctgct gcaagaccat gaatataaac
     9961 tgctgcaatt gcagcatcgg ttgcgtttaa gccttgtgca agcaatgcag taataatgcc
    10021 ggttaatgtg tcgccactgc cgccttttgc cagccctgca ttgcctgttg tattgaagaa
    10081 acattttccc tcaggagtag aaatagaagt gtaagcacct tttaagacaa taatgatttt
    10141 gtgttttact gaagcagcaa tttgaaaatc atttctttga aaatcattct caaatgttcc
    10201 gaatagtctt tcaaattctt ttttatgagg agtaagaatg gtgttttccg gaagattgtc
    10261 tatcagattt ttattttgtg aaattatatt taatgcatca gcatcaagta ccaatgtttt
    10321 ttttgctttt tgaagaagta gtttcaacat gcttgaggtt tgttcgttaa tacccaagcc
    10381 ggggcctgca gcaattgcat tgtaaggtgc taaatccggt aagtcacata ccttgtcacc
    10441 ttcacaagtg ctcaacattg cttccggaat tgcagattgt agtgtcgctc ctgttgtttt
    10501 tacggtatga actgttgtca accctgcacc acttttaaca catgcttttg ctgatagaat
    10561 tattgcaccc gcttttccat agcttccgcc aatcaataaa gcatgaccaa aattgccttt
    10621 atgtgaaaat cgttttctcg gcttgagtat tttatttatc aaactcctgt caaccagttc
    10681 ttgagttgta ggtaatgatt ccatataatg ttcatcaagt ccaatatcaa gaactttaac
    10741 ttcgccaatc ctgtgggcgt tttctgcata aagcatactt cgtttaagtc gctgaaatgt
    10801 taatgttgtt gatgcatgaa taacagcaac attcgttggc gtatgtttgt cggcaaataa
    10861 acccgagggt aaatcaatgg aaattacagg agttctggtt gaattcactt ggtgaattaa
    10921 atcagcaata tttttttcta ctgccttatt taatcctgtc ccaaaaattg catcaaccca
    10981 aaaagttgta tttttaaaag gtaggtttgc atcagtgagg taaaatattt taacagaagt
    11041 tttctgcaag cgcagaaaat ttgcttctgc atcgggactt agcttgttgc tattgtctgt
    11101 aagtaaaaca gaaacatttt ttcctgcatc ggccaatatt ctggcaatgg caagtccatc
    11161 acctccattg tttccgggtc cgcagagaat acaaatatgg tctttttcat ctgtaaactt
    11221 tgctatttca tcactgcaac aaatagctgc tctctccatt aaatcaacag aacttatagg
    11281 ctcagttttt attgtgtagg catcggccat acggaattgt tctgaagtta gtagtttcat
    11341 ctgtatataa aaccgttgtg gaaattggtg taaagataaa aacctgatat tgaaataaca
    11401 tgaatcatat tgaacaagca gtaaaacatg tagatttgcc gcacatttta aaatagtatg
    11461 gaagagaata ccaccttaaa acacacagct ttaacagcta tacatcaaaa ggcaggcgct
    11521 aaaatggtgc cctttgcagg ctacctgatg cctgtacaat atacggggat taatgacgag
    11581 catgaaactg ttcgtaatgc agttggcatg tttgatgtct ctcacatggg agagtttatt
    11641 ttaaaaggtg atgatgcact tgatcttctt cagcgtgtga caagcaatga tgcttccaaa
    11701 ttgtcagccg gcaaagcaca gtacagctat ttccccaatg aaactggtgg aataattgac
    11761 gatttaatag tgtattgtat tgatgaaaaa acattcatgt tagttgttaa tgcatcgaat
    11821 attgaaaaag actggaattg gcttcaaaag cataacaaca aaaaagtcga aatgcacgac
    11881 atctccgata aaacttcttt acttgccgtg caggggccta aggcaatgga aacattacag
    11941 aaactaacat ccatagcatt ggccgacatt ccatattaca attttgccaa aggagagttt
    12001 gcaggttgtc agaatgtgct tatctccaat acaggctaca ccggtgcagg tggttttgaa
    12061 ttgtatttcg aaaatcagta tgcagaaaaa atctggaatg acgttatgca ggcgggtcaa
    12121 ccattcgaca taaaacctgc aggtcttggt tcgcgtgata cgttgcgact tgaaatggga
    12181 ttttgccttt atggaaatga cattgatgac aacacatcac cattagaagc aggcttaggc
    12241 tggattacta aattcacaaa gaattttaca aactctgatg cattaaaaca acagaaagaa
    12301 caggggatag caaacaaatt ggtaggcttt gaaatgattg atcgtggaat tccaagacac
    12361 gattacgaaa tttgtaatgc atcaggtcag aagattggtc gtgttacatc aggaacacag
    12421 tcgccaacat taaacaaagc catcggaatg ggctatgtat cgaaagatca atctgccgaa
    12481 ggaacagaga tttttattaa aatcagagaa aaactgatta aagccgcagt tgtcaagatt
    12541 ccgtttatta aaaaataatc catgtctgaa gcaggcaaac ctatagttga agtttgttta
    12601 acaccggcac ttttctatca gtacaacaca aaggaatcta tagtagttgc cattgatatt
    12661 ttgcgtgcca cgagtagtat ttgtgtggcg ttgcaatatg gtgcacgctc cattgtacct
    12721 gtaatgactg tggaagaaag ttttcaatat aaagaaaaag gatatctgat aggagcagag
    12781 cggcagggct caatggtaga aggatttgac cttggtaatt cgcctttcag ttatatggaa
    12841 gacaaaataa ttggtcgtga tattgcacta actactacca atggtactaa ggcaattaac
    12901 attgccaatg gctcagcagg aattgttgca ggttcatttc tgaatcttga tgtactggct
    12961 gcatggctta ttgagcagaa taaagatgtg ctttgccttt gtgcaggatg gaaaaatact
    13021 tttaatcttg aagacacact gtttgcaggt gcattatcgc aaaaacttct tccacatttt
    13081 caactttcag agcatcgtga ttctgctatt gcagccatga atctttataa ccttgcaaag
    13141 catgacatgt acgatttttt aaaagactct tcacatcgta cacgattgga aaggcttaaa
    13201 attgatgaag acgtattgta ttgcctgaca cctaatcaaa ctcttgtagt tccggtgttg
    13261 aaggatggtg ttttaaaaaa acactctgac tggtaggcag aaaagtgatt taacagatgg
    13321 ttatttaatg aaaaagtcgc aacagtgact ttggtcagtt gtattataga acttaaaata
    13381 ctattttagc agaaaattat aaataaaaat gaaaaaagca ctttcagttt taagcgtact
    13441 cggactattc ctgattgtta acgcttatgc aaacagcaac aaagcaaaca cagttactga
    13501 tcttcaggta actgaaagca gtattgtagc aaatcatgac atgccatgtg atggtaaggc
    13561 agaaaaagac aaagattgct gcaaaaagaa aagcaaaaag aaaaaatgtt gtgctgacaa
    13621 agcagaagct tcagctgcat catgtgccga caaacaagca aaagcaggtt gctgcgcaca
    13681 taaaaaagct ggtgccggtg catctgatgt taagaccgac actaagtcag acgaatctgc
    13741 aaaataattt taacccacca aggtggtgca gtatttagct tgcaatgctg cgtaatatcc
    13801 cacggttagg gccgaaaatt gaaaaccgtt ctcattattg agagcggttt tttattgcct
    13861 attactaaac atttttattt gaagtctaaa actgcagtag aaaatttctt taacagtctt
    13921 gcatacagga taaacctgtt cttatctagg cgtgtaatag atgacatatc aggcagaagt
    13981 ttttttgccg aagccaaatc catttcctta gtgtaaattc tcctgagctg agaattgtaa
    14041 atagattgcg tcatctgata ctcttccgga tttcttccat ctaaaaaata aaggtagtag
    14101 tcgtctttat gtggaatacc tttaacatta caaacgtact cgaaattttc tgttgcactt
    14161 acttttcttt taataacagt ctgcatattt ttatcttgca tatcggtaca aattaaaagc
    14221 agttcgtttg cagaagtctc cctaccgatt gatgcaactt ttttaccatt cacatcagca
    14281 ggtttggtgt aggaggcaaa ttgcttttca ataagtagca taatattgcc attactcatc
    14341 acatccattt gctgaatttt taaaaagtca acgcctttaa tgttctttag taataagttg
    14401 tcaaccgttt cgagtaatgt ttcttcagag aattttatgc tatttgtttt aataagttga
    14461 aggttgttgt cgaactccga aatgcttata ccattacttt taaatttgtt tgcattggca
    14521 atatcgttat aaataggatt gaaagtattg tcagtgaaac caaataatga aaccacaatt
    14581 attctgttgc cttcatgaat aattttgaaa tcggataatt cttccttttc agcagaaatt
    14641 gtttgagtga taatttttcc ttcaacacta gttgcaatga aatattcttt atctctttgg
    14701 aatatacctg aaaaagtgcc gctatcattt actgctccat caaaaaatgt actgttgggt
    14761 aaagaagttt tccatgcact gactaactga ttgctgaaac aataaatact gtcattagtg
    14821 gtaatggtaa agaaacggtt attgatggaa gtattatagc tgaaaaaatt cttctgatta
    14881 tattttgcaa catcttttcc tgcaattaaa actactgttt gcttaaaata tccttttaaa
    14941 tcaaaaacat cagcgcaaaa ttccagattc ccaacccctt ttgaataaaa tatttttatg
    15001 gttgaatcgc tcagtacaac atcatatacc cgcaccggaa actgcaaaaa tcttatagcc
    15061 gcagtatctt ttagaataaa tttatcatcg tagcgtagta aagttaattt gagctttttc
    15121 caggctgttg ctaaaaaaat gctgtcgctg gcaccctgaa tcattttaat attgctgccg
    15181 gcagcataaa aatagccatt gtttacgcct aaaaattttg ggtatggacg gtcatttaat
    15241 tttactgtcg ggttccatat caggttttgc ccaaaggcag aaacactaca gaataataag
    15301 aggaagaaaa tgattttact ttttgttttc ataaacttta atgccatttg aataagttgt
    15361 ttctataggt aatttaaata atatactgtc cggtgcagtc atcaggtcgt cattcataat
    15421 tacaaagtct gctgctttgc ctttttctaa agagcccttt tcattctctt caaacataga
    15481 atatgctgcc caaatggtca tgccgcgcaa tgcttcttcg cgactgattt tattttctgt
    15541 ttcaaaacct tccggtggaa aacctttctg atctttacga accacagctg cataaaatcc
    15601 ataaagcggg ttaatgtctt caaccggaaa gtcgctgcct aaggctaaca tacctgtttc
    15661 ttttaataag gcgttatttg aataggcatc gtgtattctt tcgctgccaa gtcttttttc
    15721 tgcccaatac atatcgcttg tggcatgtgt tggctgaaca cttggtatta tggaatagtc
    15781 agaaaaatac tttaagtcat catgatttac aacctgacaa tgttcgatgc gccatcttaa
    15841 atcatttttg gtttttaggt tggcggcata aacttttaac ataaatcggt ttgcagaatc
    15901 accaatacaa tgcgtgttta gctgaaatcc atgctccact acttctttgg ctgccaattg
    15961 aatggagtcc ttgtcttgaa gtataaaccc ataatgtccg ggaaggtcgg agtatggttg
    16021 caacaggcag gcacctcttg acccaagtgc tccatcggca taaagtttaa acgatcttac
    16081 ctcaagtctt tcagttttat attttccttt ttcgaaccag tattttttac tctcattcgt
    16141 cagttctgcc atggcataaa ttttcatgct aagggttccg gctttttgaa gtgagtcgag
    16201 taaacgaatg tcagaaactc ttaatccggc atctgtaaca gaggtcagac caacggcaaa
    16261 acaattttgt tcgcctttta taagtccttc cgttttaatc tgagctgtaa attcaggaat
    16321 atgtgctttt acaatgtcaa cagcattatc aatcagcagt ccggttaagt ggttgttttt
    16381 agttattatc tcaccaccct gaacctttgt agaagtggtt atacctgcaa tatccaaagc
    16441 tttttggttg actaaaacgg catgaccatc aattcgcatc agataaaccg gtgtatttgg
    16501 gaacaaacta tccaaaacag acttgtcggg aaattcattc tcagaccagt cattttgatc
    16561 ccatccacgt cctaaaatcc aactgaactt attggtagcg gcatattctt ttaccttttg
    16621 tattacttca tcaaatgatt ttgtgccaaa aagcgggcat ttggaaaggt ctgtgctgta
    16681 gccataaaaa tgacagtgag catcaataaa gcccggataa acacttttac ccataaggtc
    16741 tattttctgc tggcactgat acttatctgc caatgcattg gtgccggttt ctactatctt
    16801 gccattagaa accaccattg cttgtgctgt agaaaaggtg ctgtcaacgg tataaatttt
    16861 accgttaaaa agaatcaggt ctgctattgg cttttggctg catcctatgc aaattaaaag
    16921 cagcagcagc attggtttaa aggacttaat caatgttaga attttaaaat caggtatcaa
    16981 atatccataa aatctttaaa agaaaggcgc aacttagaaa caggcaggta ttgaaaaata
    17041 ttttttgatt tgttcttgca tcaattaaaa cctgctgtat atttacgcaa aaaatagaac
    17101 acatactcac catcaatctt gtttacctat ggcaaaagca tcaaaatcaa aaaaatctgc
    17161 tgctaagaaa aaagcaaaac ctgcccgcaa agcaagtggt aaaaaggcag ctaaaaagaa
    17221 acctgtagca aaaaagaagg ctactaaaaa gaaagcagca ccaaagaaaa aggcagctac
    17281 caaaaagaag gctgcgccaa agaaaaaggc agctactaaa aagaaagcag caccaaagaa
    17341 aaaggctgct accaaaaaga aggctgcacc aaagaaaaag gcagccacca aaaagaaagt
    17401 agcaccaaag aaaaaggcta ctactaaaaa gaaagcagct ccaaagaaaa aagctgctac
    17461 caaaaagaaa gcggcaccta aggttgtaat aaaaaaagtg gtatcaaaac cggcagtgaa
    17521 aaaacctgcg ccaatgccaa aagaaaaacc tgaggatatt cagcctgtgg taacatacca
    17581 aacagaaatt attgaaatag ttaggccggt agaaatggat atggatgata atgatgcaaa
    17641 gatggacgag aaagaaatga tggacgatga tgtgtcattt aacgatgatt tattcagcgg
    17701 ctcatcagac gatgacgatg atgatatgta aaatgtaaat aattaatatt taaaaaaagc
    17761 tctgcattgc agggcttttt ttgttgactt aagtatgatg atttttatcc atttattaat
    17821 ctttaagaaa actctaccca acagtcctct cattccccgt cacttttact ttttacctta
    17881 aatttctcaa ctaacaattc ggaattatca gattacttca tcacgtttca cccctgtcag
    17941 ccgtggctga tcatcacccc agtcaactgc ggctaatcat cactcaaaaa ttgtccgacc
    18001 tctaaattca aggcgacagc gttgccttac tccattgatt attgttcaat gtataagcta
    18061 ctctgtcgtg gaggcggctt tgcctgcctt gccagaattc aaaataatcg ggttttaaaa
    18121 taaaacctcc ccagtgtgga gggcgcatta cctcgctgcc catactttct tttaatgata
    18181 aaaacttttt ttcaagtacc aaacgcgact caataatctg actttgctct gatgcccatg
    18241 caccaatacg actttctaat gggcgtgata aaaaatattc gtcagaaact ttcgtgtcaa
    18301 ctttttcaac cttgccttca acacgaattt gtttcgccaa ttcaggccag aaaaaattta
    18361 atgctgcata aggattttct atcagttgat ttccttttct gctgttgtaa ttggtaaaga
    18421 aagtaaaacc ttcttctgtt gcatcgcgca acaaaacaat tcttgatgat ggcctgccgt
    18481 tttttgtaca tgttgcaaga ttcatggcat ttacgtcagg taaatttgcc tgtgcagcaa
    18541 ggctaaacca cttttcgaat tgtactatcg gattgtcatc agcctcttgt gtgccgaacg
    18601 tgtctgcctc atagtcactg cgcacatttt ttaaaacttt atgtatatca tccatgatta
    18661 atgtcatttg aaatgcaaat ttataaatgt aagatatatg tattattcat cctttcttta
    18721 acataagtag ccttgttatc agcatgttga aaagacagtg gcatgttttt agcaaactga
    18781 tttattaaaa gagattgtta acaacaagta actttttcag acagatacgt aaaatataac
    18841 aaattaattt agtgttctat ggaagatttc aatcaacatg cagctacagg gtcactgtta
    18901 atttcagaac ctttacagtc cgactcattt tttaaacgct cagttatttt actaagcgaa
    18961 catgatacag aaggcacgtt aggctttata ttaaataaac tcacagacgt tacaattaac
    19021 gatgcagtag aagatttccc acactttaat gcacctttat tctttggtgg tccggtagaa
    19081 acagatacat tgttttatct gcatactgtc ggccatcttc ttaaagattc gaaagaaatt
    19141 gtaaaaggag tttttttagg tggtgattat gatgagttga aatttttaat caatacaaaa
    19201 caaatcagcc ctaatcagat tagattttat gcaggctact ccggttggaa acccaatcag
    19261 ttagaggacg agttgtttca caatgcatgg atgcttggta agggtaatgc ggaatttaca
    19321 ttctatcctg agcctgaagt tttgtggagt caggtattgc gcagcatgaa aaacaaatat
    19381 tctttgctgg caaatttccc tgatgatcct actttgaact aagttagatt agttataaga
    19441 aatcagaata aattatttaa gtccattgta aaaacttatt gggcaagcag tccatcacga
    19501 attgtaattg tcctgtcggc catttctgca agttgattgt tatgcgtcac aataacaatg
    19561 gtttttttat aatcgtttcg cagtttcaaa aacagtttat gtaagtcttc tgcattgcct
    19621 gaatctaggt ttcccgaagg ttcatcggca agaataaaat caggattgtt aattaatgcc
    19681 cttgcaacgg caacacgctg ttgttcgcca cctgatagct gtgatggttt gtgatgcatg
    19741 cgctgttcaa gccctaactg aatcaacaac gcattgcttc gctttaaagc actcgactca
    19801 ctttccccgg caatcatagc aggaatcata acattttctg cggaagtaaa ttcaggcaac
    19861 aaatgatgaa actgaaaaac aaaaccaata tgcttattcc tgaaggcagc taatttcttt
    19921 gaagaaagtg ctaaggcatc tgtattgttg agcaacactt ttccgctatc cggcttgtca
    19981 attgtgccga taatatgcag cagcgtggac ttcccggcac ccgatggacc tatgatggct
    20041 accaactcat tggttttaat ttcaagtgaa atgccttgaa gaacctttag cccgttatat
    20101 gctttatgta tattttcaat gaatatgctc acgcagctaa aataagtatc attccaatac
    20161 tattatattt gcccctcatc gttttgtttt ggaaataata cttgcaacac catattggta
    20221 tattttactt tgtctgctgg caggcttggg ctgtgcatgg tggctttatg gtggtaattc
    20281 accctttaat gaaacacctt ggctgaggcg tactttattc tttcttagat ttttatcctt
    20341 ttccatcatt gcttttctgc tgttatcacc atttattaaa tctgccatac gcgaagttga
    20401 aaaacctgct attgccattg cttttgataa ctcttcgtcc atattgcttg attcaacaca
    20461 tttttcggca tctgcattaa atcaactggc agagaaactt acttcagcat ttaaaaacga
    20521 ttatgaagcc ggaagatatt cttttggcag taaggttgac aatgaagtta ctgataatga
    20581 tttctctgag aagagtacta acatttcaga gatgttcgat tatttaaagt cactctatgg
    20641 cggacgaaat ttgggtgctg taattatgtt gtccgatgga atttataata agggaagtaa
    20701 tcctgtttat acagcagaaa aattaaaagt tcctgtttat actattgctg ttggcgatac
    20761 aaccatcaac cgtgatgtgt taatatcaga tgttgcatac aataaagtgg cttttctcgg
    20821 aaataatttt cctgttgaag taggtgttac catgaggcag gcatcaggaa cagcagttac
    20881 gctcaccatt aaagaagatt cactgacact tttcagcaga atgatttctg catcaggaaa
    20941 taattatttc gcaaaaatac ctgtcatact ttctgccaat agaaaaggta ttcataaata
    21001 taaaattcgc atttctgaag tggatggtga aaagaatttg ctcaacaatg aagcagatat
    21061 ttatgttgac gtttcagaac gcaaaatgaa aatattgttg ctggcgcaat cgccacatcc
    21121 tgatattgct gcaataaaat ctgtgcttga taaaagtaat ctgtatgaaa ccaaagttat
    21181 ttttcaggag caagccgatg aaaatttcag caactacaat cttgtaattc tgcatcagct
    21241 tccatcggca aagtcaacaa cacttttgca aaagattaaa acggcagagg tgccttactt
    21301 tatcattgca ggtgcgcaaa tgaatgtcgc tgcattcaac aattccggta cgggtgtaac
    21361 aatcaatcag cctgttgcaa agatgaatga agtatatgct aaggtaagcg atcagtttgg
    21421 attgtttagt ttgtcagacg atatgcaaaa agtattgtcg caactacctc cattatttgt
    21481 gccttttgga aattataaag tagagaaaga aaccaataca ttattagcgc agcgtatcgg
    21541 aagcatacaa tcgtcaatgc cactgtggta tttttctact tcaggaacaa tgcgaacagg
    21601 tgttttactt ggggaaggtt tgtggcgctg gcagttgaat gaatatgcac tttcaggaaa
    21661 tcatgatgtt actgatgagt taattttaaa atctgttcag tacctgactt caaaagagaa
    21721 aaaagagcct tttatgatac agtacaagcg ctcctataca gaaggtgaga atattgagtt
    21781 tgatgccacg ctttataatg cagccggaga attaattaat gaaccggagg tgcgcctgaa
    21841 tatttataac gatgagaaaa aagagttccg atacactttt aacagaagca acaatgccta
    21901 tcagttgaat gcgggaatgt tgccaccggg acgctatact ttcagtgcgg atacaaaagc
    21961 gggcgataaa gtttataagg aaacaggaga gttttctgtt gtgccattgt tgctggagaa
    22021 ttttgaatca actgccaatc atcaactgct caataccatt tcatcaaaca caggaggcat
    22081 tatggtgact cccgataaaa tcaatttgtt gattgatgct gttcagaaaa acgaaacggt
    22141 gaaacctgtt tcgtattatc ataagaaact tattgaattg ataagcctga aaagtttact
    22201 tgcgatctgc ttgttgttaa ttgcaatgga gtggttctta agaaaacgtt tcggttctta
    22261 ttaaagattt tttttctcga ttttcttttt catttcttcc ggtgtaatcc aaacggcatc
    22321 atcatactgc ctgaaccatg tcaattgtct tttggcatag ttgcgtgtat gttgttttat
    22381 tttttcgatg gcttggtcaa gagtaatgtt tctatcgaaa tactcaaata attctttgta
    22441 gccaacagtg tgcaatgccg taagatgccg gtagggtaga agtagtttcg cttcatccac
    22501 caatcctttt tcaatcatca tatccacacg ttgatttatg ttatcataaa gtaattgcct
    22561 gtcgggattt aaaacataaa gtgtgaagtt gtaagtagat attttgtttt tatcttcaat
    22621 aaaagatgaa cgtgttttgc ctgagctgta aataatttct aatgcacgaa tgaccctgcg
    22681 tggattgtga atgtctattt gatgatagct tttttcgtca agagttttta attgatgagc
    22741 cagatactcc aatccttttt cagcacactc tttttcaaga cttgctctaa gttgataatc
    22801 ggtatttgga gtagggtcga ggccgaagag agcagcatgc atatacatac ctgtgccgcc
    22861 tgtaagaaca actgtttcat ttttttctaa caactgattg atggtgttag ttgcttcatc
    22921 ggcatagcga cttgcattca tatcttctgc aatagaatgc gaatgaatga agtgatgttt
    22981 cactgtattc atttgttggg catctggcac ggcagtgccg atagtcattt cacgaaaaat
    23041 ctgtcgcgaa tcgaaagaaa caatttctgt gctatgcagt ttagcaactt caatggcaat
    23101 ttgtgttttg cccgaagctg taggccctgc aataataatt aactttttat ccttaggcaa
    23161 tttcttattc tttgcttatg gcaaagttaa aaggtaagtg taatagtgcc tttggtatct
    23221 aacggacatt tagggcagtt attaaacatt acactttctg ctgcctgttt tgctttagtt
    23281 aacaaatagc tgcttgtagt ggttgtgcct tctgcacctg gattagcctt ggtaacatgg
    23341 ccatatttgt caatagaaat ctcaactaca acaacacctt tttccatagt aagattttca
    23401 atctttggcg gcttggtgat ggtacgtccg ggcatttcat attttacacc acgcatatcg
    23461 gcactcatct ctgagttgtc tgcttttgtt tctgatgctg catcaaaaaa tatggcagag
    23521 gcttcactca ctttaaaatt catgggattg cctttaaata aaaagctgat attgccatct
    23581 ttaatctgct gcactgtgcc tgtcattttt tcgccacttt tcaaaacaag ggtatgttgt
    23641 gcaaataaga aactagcact gcataataaa agcgcctgtg ttgcaagaat ttttctgatc
    23701 atgatttaaa cttttttcaa aaataaattt ttatagctgt tctaagaatg aatttgaatt
    23761 gaaatatgaa aacagttttc aacaatagaa gctatattag gcatcttcga tacagaatga
    23821 taattttggc accataaagt agtaaaatat gaaattagta acctatcaga atcagaataa
    23881 aacggcattg ggaatcttgc ttagtgataa agtttataat cttagcgaat tgtcacaacg
    23941 cagaggaaaa actgtgttgc ccgacaatat gcgtgatttt ttaacaggcg aacaactaat
    24001 gatggatgct gctatggagt tgaatcaatg gctttcagat ggcaatgcag atgctgacgg
    24061 actctcagta gctaatgtaa atttattggc accggtgcct aatccaactt cttgccgtga
    24121 tggttatgcg ttcaggcaac atgttgaagc agcaaggcgc aacagacgtg tagagatgat
    24181 tccggaattt gatcagtatc cgatttttta ttttaccaat cacaatgcta tacaaggccc
    24241 tggagatatt ttttgcatgc ccgatcattt tgaacaactt gacttcgaac ttgaggcagc
    24301 aattgtcatt ggtcgcaaag gcattaatgt aaaagcaaaa gatgccgata gctacattgc
    24361 cggctatatg attatgaacg atatgagtgc gcgcaaattg cagatggaag agatgaaact
    24421 caacttaggc cctgcaaaag gaaaagattt ctctactgtt attggtccgt atttagtaac
    24481 gcctgatgaa cttgcatcat ataagaccat tgcaaaaagc ggacacacag gaaataatta
    24541 caatttaaaa atgacttgta aggtcaatga cattcaggtg tcggaaggta atgtaggaag
    24601 tatggattgg acatttgcag aaattattga gcgttgtgcc tacggtgtaa acattcttcc
    24661 cggtgatgta attggcagcg gtactgttgg cacaggatgt ttcttagagt tgaacggcac
    24721 agggttattc aataatcctg actataaacc acagtggctt aaagaaggag ataaagttga
    24781 aatggaaatt gccggtcttg gtgtgctgac aaataccatc agaaaaatag attctgattt
    24841 ttcaatactt gcattgaaga agatgtagga gattgaaaaa gaagaatgct gtttttattt
    24901 aggaactatc agcaagagga tatttttagt cctttccatt tattgtaatc attatttctc
    24961 tgaaactttc cagtccagct tcaaggtttt caattatttc tcctgccaaa acatccgggt
    25021 cgggtaagtt gtcaaggtcg gcaagtgact tatcttttat ccaagtgatg tcaagcgatg
    25081 tcttgtcacg gttgataatt tcatcataag tgaactttct ccaacgacct tcaggatttg
    25141 tgtctgagtt gtaagtttcc tttcttttgt gtcggttgtc aggattgtag caagtaatga
    25201 agtcccgaag gtcttccaat ttcagcggat tctttttcag cgtaaaatat tgtcctgcac
    25261 cctttttgtg tcttatgggt tattctctaa tagaccttca taaatggttc cttttatgtc
    25321 tgctcccaat gttgcccact gctctttatc aatcatgtca atgattttgt aaagcatggc
    25381 aggttcttta atcttattct gactttgaac aaatatttgc cccaacactc ctttttgcct
    25441 tgacaaaaca cttagggttt tggtatgata ttattccagt tcatcaccac gccttgaagt
    25501 caaactttcc caattgtttt cagttgggat tggcagtttt ctgttgtagg ggggagatgc
    25561 tgaattcata agtcattttc aaaaacagca aataggtaag ttgttctaag tagtcgccat
    25621 aaccaacgcc aatatctctt agcggattac agaaactcca taccttactt actattgctg
    25681 atgtattttc gttgctcata tatttcgttg ttttcacatc aataacccca aagggattat
    25741 agctttatag cattaattcg gtttgtaaaa cgcgatgccg taggcatcga actattccat
    25801 ccattcaaac aaatattttt cattgtaatc aacattaaac ttctttaaaa attcaacata
    25861 ttcttcttta aaagatttct ttcggtgatg ttccttttga ttggtgatgt atgcaataac
    25921 atcgttcaac tgggaatggc tatacgaaaa agcaccgtag ccttcttgcc aactaaactt
    25981 gaattttgat agcttttcaa ctttgataaa atcgtttgat gattttttta tttctcttac
    26041 caaatcggat aaacaacaag atggtttcat gccgataaga aaatgcaaat ggtcggacat
    26101 gccgtttatg gcaagcattt tttgttcttt gttttgaaca atgccagtaa tgtatttatg
    26161 taaacgctct tcccattgtg gcagaataag gctttctctt ccttttacgg caaaaacaat
    26221 ttgaatataa atttgcgagt aggttgacat tatttatggt ttatgttcga ccccttcgga
    26281 gtcgtgttgt ttgttggttt tgtttttcta taaaggttgg atgcctttgg catctttagc
    26341 caaagttaaa taaaagtttt gctatttaag tttgtagtca ttaaggttaa ataaagactt
    26401 tcttatttaa ccttcattct ttccatactt cccttttcca ttcttcattt tctttatccg
    26461 ctccaacaag gctaaagcag gttcatcatt ggggtcttga ttcacttata ttaattaggt
    26521 tttagattaa tttaagttac tgttttggtt gacacgaata atgattttac tattaattac
    26581 taaattgtaa tttacatatt cctcctatac tgtccgccaa cttcaaacaa tgcattggta
    26641 atttgtccta aggaacaata cttacttact tccatcagct tttcaaacat attttgatta
    26701 tggattgcag tgagttgcag gtcgtgcagc atttcaggtg cagtagatgc ataacgtgca
    26761 tgcagatttt ttaaagtagc aatttgctgt tgcttttctt cctctgtggc gcgaataact
    26821 tccttaggta aaatagttgg tgagcctttg ctcgataaaa atgtattgac accaataatc
    26881 ggataagcac cggtgtgttt taacgattcg tagtaaagac tttcttcctg aatttttcca
    26941 cgctgataca ttgtttccat tgcacccagc acaccaccac gctctgtaat gcggtcaaac
    27001 tccgacagta ctgcttcctc taccaaatca gttaactctt caattataaa tgaaccttgc
    27061 aaaggatttt cgtttttggc aagacccaat tcgcggttga tgataagttg aattgccatt
    27121 gccctgcgca cactttcttc tgtaggagtg gtgatggctt catcataagc attggtgtga
    27181 agtgagttgc agttgtcata aatagcatac agagcctgca gtgttgttct gatatcgttg
    27241 aagtcaattt cctgagcatg caaacttctg cccgatgttt gaatatgata tttaagcatc
    27301 tgcgaacgtt cattggcctt gtattttagt ttcatagcct tagcccaaat acgccttgct
    27361 acacgtccga taacagaata ttcagggtct atgccattgg agaaaaagaa tgaaaggtta
    27421 ggcgcaaaag aattgatgtc catgccgcga cttaaataat attccacata agtgaaacca
    27481 ttactcaatg taaaagcaag ttgcgaaatt ggattggcac cggcttcggc aatgtggtag
    27541 ccgcttatgg aaacagaata aaaattacga atggctttgt caataaaata ttgttgcatg
    27601 tcgcccatca atctaagaga aaactctgta gagaagatgc aagtgttttg tgcctgatct
    27661 tcttttagaa tatctgcttg tacagttccg cgaacctgct ttaatgtgtc ggtttttatt
    27721 ttatcgtaaa catcttttgg aagaactttg tcaccggtta ttcccagaag cataaggcca
    27781 agcgaattgt ttccttctgg aatttcgtag ttgtattttg gttggcttga tttatttttt
    27841 gaaaattcgt cagcaatttt tttctcaact tctttttcta atccgttttg tctgatataa
    27901 atttcgcatt gctgatcaat agctgcattc ataaaatagg ctgcaataat tgaagccgga
    27961 ccattaatgg tcattgatac agaagttgca gggtcgcaaa gattaaatcc gctatacaat
    28021 ttttttgcat catcaagaca acatacactt acaccactgt ttcctatttt cccataaata
    28081 tcaggacgat ggtcagggtc gttgccatag agtgtcacac tatcgaaggc agtgctaagc
    28141 cgtttagcag gcatgcctaa tgaaacataa tggaaacgtt tgttggtacg ctcaggacat
    28201 ccttcaccgg caaacattct tgttgggtct tcgccttcgc gtttaaaacg gaatacacct
    28261 gcagtaaacg gaaattcacc gggaacattt tcctgcaaaa tccatttcag caaatcgccc
    28321 caagctttgt atttcggaaa agctacctta ggtattttgt tgtgcgaaag tgattcatca
    28381 aatgttttta tttttaaaac tttatcacgt actttaaatt cataataatc tttactgtac
    28441 tcttctgttt tagcaggaaa atcttccaat agttttttgt tgttgccatc gaaacttctt
    28501 tcaagtatat catattgttt tttcagctct tcggcagctg ctccttttat tatttgcatg
    28561 ctttcataga gcgagtaaag cttctgtgca agtgtacatt gttcttcaac ccactggtca
    28621 taatgtctgt tgttttctga gatttcagaa agatagcgtg tacgatgcgg aggaatgata
    28681 aacacttttt cactcatctc ttctgtggtg tgatagtcgc tttttaagtc tgctccggtt
    28741 tttgctgcaa tggtattcat cacagactta taaagttgat tcatacccgg atcgttaaac
    28801 tgagaggcta ttgttccgaa gacaggaatt ttttcatcac caatgtcaaa caacttatga
    28861 ttgcgcttgt attgtttttt tacatcgcgc aatgcatcta atgctccgcg tttatcaaat
    28921 ttgtttagtg caatgaggtc ggcaaaatca agcatgtcaa ttttttccaa ctgtgttgct
    28981 gcaccatatt ccggagtcat tacatataaa ctcacattgc tatgttcggt aatctcagta
    29041 tcgctttgtc caatacctga tgtttcaagg ataattaaat caaactgtgc ggcttttaca
    29101 atatctaatg cttcctgaac ataacgactc aatgcaagat tactttgacg agttgccaac
    29161 gagcgcatat acacacgtgg attgcgaata ctgttcattc tgatacggtc gccaagtaat
    29221 gcaccacctg ttttgcgttt actggggtca atggaaatga tggcaatata tttatcttta
    29281 aactccaaca aaaatcttct taccaattca tcaacaatag atgatttgcc tgcgcctcct
    29341 gttcctgtaa ttcctaatac aggactgttt ttggttttat tagagaggga agaagtaatt
    29401 tcaggatata gtttttcaaa ttccgattga ttattttctg cacaagaaat gagccttgca
    29461 atggcttttg aatttttctt tgtcagctct tttatttcgc catttaaatt tactcctgtg
    29521 gcaaagtcgc atttttggag catatcgtta atcatgcctt gcaagcccat agcacgtccg
    29581 tcatcaggat gatagatgcg ggcaatacca tagtcctgaa gttcgcttat ttcttctgga
    29641 agtattgtgc caccaccgcc accaaatatt tttatatgcc cacagcctct ttctttcagc
    29701 aagtcataca tgtacttaaa atattccatg tgtccgccct gataacttgt catggcaata
    29761 gcattggcat cttcctgaat ggcacattcc actacctctt gtgcacttct gtcgtggcca
    29821 aggtgaataa cctcagcacc acttgactga atgattctgc gcatgatgtt gatggcagca
    29881 tcgtgcccat caaacagcga agcggcagtt acaatacgga ttttatgttt cggggtataa
    29941 atttttgact cctgcatatc agaataacct ttatgctgca aatttaacat ttacagcgta
    30001 agcaggaaag gggaggtgta gttatcttat aagaatgaaa aacacaccta tagctaatta
    30061 aaaattgcga aattgttgta gggttacaac aagcaaaggg caatcctata aatgtatcac
    30121 ttcattatag gcggcagcag cagcttccat aatagcctcg ctcattgtag gatgcggatg
    30181 tacagccttg ataatttcat gacctgttgt ttctaatttt cgtgccagaa ccaactctgc
    30241 tatcatttca gtaacgtttg cacctattaa atgtgcacca agcaactcac catatttttc
    30301 gtcaaaaata agcttaacga aaccatcact ttgtccaccg gctttagctt ttcctgaagc
    30361 actgaatggg aacttaccaa cttttattcc tatgcctgca tctttagcag ctttttctgt
    30421 catacctaca gaagcaattt caggagtgca ataagtacat cccggaatat tgttgtaatc
    30481 gagtggttgt ggatgatggc ctgcaatttt ttcaacacaa ataattcctt ctgcagaagc
    30541 cacatgtgcc aatgcctgac cttttacaat atcacctatt gcgtaaatgc caggtatgtt
    30601 ggtctgataa aaatcgttta caacaacctt acctttatcg gtaatgatgc cacactcttc
    30661 aagacctatg ttttcaatat ttgttgttat gcctactgca gaaagtactt tttcgcaggt
    30721 aataactgac tcaccttttg cagatttaat ttttacactg cacgttccgc cacttactgt
    30781 aaccgattct acagaactgt ttgtcatgat ttcaatacca acttttttca aagagcgttc
    30841 taattgcttg ctcacttctt cgtcctcaac aggaacaatg ttaggcataa attctaccac
    30901 agtaactttt gtgcccatag cattatagaa gtaggcaaac tctactccaa tagcacctga
    30961 acccactacc accattgact ttggttgttc gggcaggctc attgcttcgc gatagccaat
    31021 aatgtttttg ccatcttgct tcaggttagg caactcgcgc gaacgtgctc ctgttgcaag
    31081 aataatatgc gctgctgtat atgttttttt gctgccatcg gttgctgtta cttctacact
    31141 ttttccgggc tttacttttc caaaaccatt gatgacttct attttatttt ttttcatcag
    31201 aaactgaacg cccttgctca tgccatctgc aacgccacga cttcttttga taacagcatt
    31261 aaagtcggct ttgccacctt caacagtaat accatagtca gaagcatggt tcaggtattc
    31321 aaaaacactt gcacttttga gcaatgcctt agtaggaata caaccccagt tcaggcaaat
    31381 accacctagg ttttcttttt caataattgc tgttttaagt cctaactgcg atgctctgat
    31441 agctgctaca taccctccgg ggccacttcc tataatgatt aaatcaaaat tcataattga
    31501 tgctttaatt tgctgctaaa atatagcttt ttaatgaatc tgatagcagg tattaacttt
    31561 gtttctgtaa atgtgtttgt acatattgat agcgtggagg tatcaaataa attttgtcaa
    31621 tgaaaatcag aatatttaat attgccttag ttaccttaat gggaatgtgg cagctaagca
    31681 ttgcgcaaaa cagcagaaaa atggaataca ataaattgac acctcaggag gagaaagtgt
    31741 tgttgtttaa agtaacggac aggcctttta ccggtgatta ttatcttaaa aaagatgtag
    31801 gcacttatat ttgtcgcagg tgtaatgcgc cactttatag aagcgctgat aagtttgagg
    31861 cgcattgtgg ttggcccagt tttgatgatg aaataaaagg ctctgtaaaa cgaattcctg
    31921 atgcagatgg tatgcgcaca gaaattgttt gcagtaattg tgacgggcat ttaggtcatg
    31981 tttttttagg cgaaggattt acagctaaaa acaccagaca ctgtgtaaac accagctcat
    32041 taaaattcat acctgcagga acacctctac ctgaagtaat caaaaagtag taagcttatt
    32101 ttattaccgg tatcttaatg ttgagcgaag cgttgacact attaatgttg cttaaataaa
    32161 ttcctgaggt aagattaata ggtagtttga atgtgttgtt acctgatttt aaactcaact
    32221 tccaagctgt aattaaagtg ccttttaaat cataaagata aagggttgcc tgattgttgc
    32281 tttttgaagt taaattcatt tgaagtgatt gctcattttg attgaaagac atgtcaacat
    32341 tatttacatc attaccatat tcagaaatag aagtagttgt gtctgtaacc tgatacacct
    32401 tctgattcac taagtctgca atatacaatt caccattatc atcttcacca aaggtgctga
    32461 atgcacgtcc gggaaatgtt cctgcaacgg taacggcatt gccgttgtta gggtctaaag
    32521 catatattgt tccattacag aaatcggcaa agaaataata tccataaaga gcagggtagg
    32581 cagtgccacg atatacaaat ccgcctgtaa cagaacatcc accggtagat gcatggctat
    32641 atacgtggac aggtgaaata taatttccta tgcaccctac acttgtattg taagtaacta
    32701 aaccttcgta gcagcgccat ccgtaatttt cgccacctgt tgaattagcc tgttgaaaat
    32761 ctatttcctc ccatatatcc tgacctacat cgccaatcca catgtcatgt gtaagtctgt
    32821 caaaactgaa tttccatgga ttacggaatc cacttgccca tatttcattc ttaacattca
    32881 taactccaaa aaaaggattg gttgatggaa tggtatatgc tccaggctgt aaaacatcta
    32941 tccttaaaat tttaccaaaa tacatattca tattctgtgc tctgttaccc ggatcgccaa
    33001 catcaccacc atcaccaaga gcaagataca aatatccgtc attaccaaat ttcaggcagc
    33061 caccattatg attcgtataa ggctgcgcca atgcaaataa aatttctgca cttacagtat
    33121 cggcaacatt tggatcagca cttcttttgt atcgggctag cacactgttg ccattaccag
    33181 ttttgttggt atagtaaacg taaaaactac tgtctgtggt gtaatttggg ctaaaggcaa
    33241 gacctaataa tccctgttca cctgtattac ttaatgatgt tggggttact ttactgttga
    33301 tgtctaaaaa aggaatggta tctgtcacac ctccggggtt aattatataa atgtatccta
    33361 gttgagttgc tgcatacatt ctgccatcac cattggttgc aagatccgat gtcttgccat
    33421 aaatgccatt agcatattca ataatattta cctgtggttg tgcccaagtt tgactaactg
    33481 tagccataca taaaagcaga atagaaacgt ttgcggaaaa tttcattaat tgtgttttaa
    33541 tggaaaagca aatatagttt gtgggctaag atttttttgt agtaaattgg taagttttgt
    33601 gaaaatttac tcaccgtttt tttaaacaaa aaatgctgac cgtaatcagc atttttatca
    33661 aagaaatata tttttcaaaa acgtcaaata gtttatttaa ttacgttgat tgctccatgg
    33721 tattttttag aagttccatt gtgcaattgg atttctatct cataataata gactccggca
    33781 tgattatctt ttgcatccca gccattcttc cagtctgaat aagatgttac ctttttgccc
    33841 catcggttaa agattgttgt ttctgcactt ttaatagcag tactgctgta agtgaataca
    33901 tcattctttc catcattatt tggtgtgata acatttggaa ttattaatga gtcgtcaatc
    33961 acttcgataa tagcagatgc agtgtctgta caacctgcat tgtctgttac ggttaacact
    34021 acgttgaacg tgccaccata attaaaaatg ttagatgcat tgttgtttgt agaagtgttt
    34081 ccatcgccaa aggcccatga tgatgcggtg tagtttaaac tattgttggt aaaatctact
    34141 gtcagaggag cttggccact ataaacattt gctgcaaatg ctgcatcaat gtttgttgct
    34201 gtaattgtaa aattggctgt atcgctgcca cagctatttg ttgcagtaac tgcgtaaatg
    34261 cccggtgtgt tgacagttgt tgttgatgtc gttgtgccat tgctccagag gtaattatca
    34321 ccgcctgttg ctgttaatgt caacatgctg ttgttgcaga gcactgttga tggtgaagaa
    34381 attactgctg ttggtacaga tccttgtgca agattgtaag tcgcactggc agttccacaa
    34441 gttgatgtgc cggttacagt atatattcct gcagtgctga ccgttataga gcttcctgtc
    34501 tgtccgcctg accatgaata ggatgaagca cctgaagcag tcagagtagc actttgtccg
    34561 gggcagatta ccgttgcaga agaagtaaca gaaactgatg gtgtgttggt gacagtaaca
    34621 ttcacaacaa cagtgtcagc gccacaagca ttagatgatg ttacagtata gtttcctgca
    34681 gtactaacat ttatggatgc agtagatgca cccgttgacc aaagataggt tgcaactcct
    34741 gatgcagtta gtgttgcaga ctgtccgcta caaatattta ccgatgtacc cggtgaaaca
    34801 gatattgaag gtgctgtgcc tgcagtgatg gtgattgtgt ttgatgcagt accacaagag
    34861 ctggttccgg taactgtata agttcctgca gtattaatcg tgattgacgg agttgtttgc
    34921 ccacctgacc aaacataggt gcttgcacct gatgctgtaa gtgtgagact ttgtcccgga
    34981 caaatttctg ttccgctatt tgaggttata tttacaacag gtgttgtagt ttgtgtcaca
    35041 ttgatagtca ctgatgatgt tccgcaggca tttgttgagc ttacagtata ggttcctgct
    35101 gtatttacag aaatagatga tgttgttgct ccggttgacc attgataact tgatgcacct
    35161 gaagctgcta aagtaaccga ctgacctgca caaatgctga catttgttcc cggtgtaaca
    35221 gtaatattag gtgcagtgcc acctgttatt gttgctgttg ctgtaggtgt tccgcatgtt
    35281 cctgtcactg ttactgtata agttccggga gttaaaacgg taatagattg agttgtggca
    35341 ccgttactcc attggtatgc tgttcctgat gatgcagtca gtgttatgct gtctcctgta
    35401 caaacagtag aaggacctgt gattgatgca ggctgaccat tctgaacaat taagtaaatg
    35461 ttacttgtaa tagtgtcatt gcattggccg agcatggaaa actgtatggt gtctgtgcct
    35521 gtaaatgttg cattggtttg gtaggttgtg acaagattgt taggagtact caaagtgcca
    35581 ttgccaccac tccagaaata tcctgaataa tttcctgtaa gattacttgc tgttaaagta
    35641 actgaattgc cggagcaaac tgttgtagca ctactttgta atgtagcagt taaagaaata
    35701 atcggagcct gacagccatt gttataatat gttggatttc ctaaagaatc aaatcctaca
    35761 ctagagccat cttgtgcggc tgtatctaat tgtccgaatt gatttactaa ttgatttgga
    35821 aaatattgta cagtttgact acaaacagca ccaaagttca ttgttaatat acgtggtgtt
    35881 gtaactgttg ctgaataatt tctgaaatgt ccttgtgtat ttcctgcgca ttgaaaaatg
    35941 acatacatcg tatctgtcag atttgcaaaa gaattgaagg tagtattaaa gttttcactg
    36001 gtgactaaaa ttactttact gttttgtggc aaaatatcat ttgatggttc aagcagataa
    36061 ccacatgatt gaatagtagc attcaaatcg gctacttttt gtgctgtgtt tgcattctgg
    36121 caaatgccaa gaaaattaag gttggttgat ggccatgaaa catttaatga gcttacctgt
    36181 aatggagaag cacctacggt aaaacgaacc atttcatttt caccctcagg attcccgcag
    36241 gcatctacaa gaatactgtt aattgtaaaa cagctttggc tataacctgc tgaataggtt
    36301 aagatacaga taaaaagtag cagaaaaatc tttttcattg atataatatt tgagatataa
    36361 ttttaaaatt cagaagtgca aaagtgcaga atttaactca tttctgatga aacttaggat
    36421 aaaaaaatat tcagtttaat aaggcattat tttccttttt aaagaatatt ttactgtgtc
    36481 tttctttccc ataagcttgg catcccatgt attctgttct gacagtgaaa aagttatgcg
    36541 gttatagggt gtgatttttt cattataaaa agtcatggaa gtagaattgt gcccttcggc
    36601 ctcccattgc agaattttaa ggctgtcgct gtcattgatg taataaagat ggttgtttct
    36661 taaaacaatt ttggaagttc cgccaaaaac ctgtttttca ccctcaaagt ggtagttgtt
    36721 attgataaat gcagtgtctg attgtttttt ccattcttca tagaatatat taccatttct
    36781 tgcagtgcct tcccattttc cgattatcca gtctaaatcg cttagcttgt tcttctcctt
    36841 gcatgacgat gtaacaacaa aaattgatag aataagaaaa taaaatttct tcatgctgca
    36901 aaaatgcaga aataatccaa ctttaggctc actacttatt aatttttaac gttgattaag
    36961 aaatgcaata tttaattttg cagcaattca gaaagtgaaa ccggaaagta tgttgcaaac
    37021 tttttttaag attaatgtgc tgaataggca gtttctattg gcttgtttat tcattttttc
    37081 ttgtagtgta aatctaactt tcgcgcagga aatgaatggc attgcacaca gtaattttgc
    37141 aggaaatatg ggtattgaaa tgaatcctgc ttccattgca actatgccat atcgtttcga
    37201 gtttaatatt ctttcaggag atattttttt tcagaatgat tatatttact ttccggcatc
    37261 agacattcca acaggaaagt ttgcaagatt tgctccgttg cgtcatgagg attatagtga
    37321 ccattatgat aaaacaccta agaatgctta tggtaatttg ttgctcaaag gcccatctgc
    37381 tgtatggcgc aatgaggaag cagctttcgg aattcatttt tctttacgca gtcagttaag
    37441 tgcaataaaa gtaccttatc atttggctaa atttctttgg gagggtagtg attatagtcc
    37501 acagcataaa attccttgga attcaactgc attcagtgca ggggcgttgg tatttgccga
    37561 agccggattt acttttgcca agacaatttt aacaaacaga caaaatatta caacagctgg
    37621 tggcacagta aattttttat tggggatgag cggtacttat atcaattcaa ctgtacttga
    37681 ctattatttc acagatgcgt cattattaaa tattaaaaat attgaagttg attacggtca
    37741 ttcaattcct aatgatgata aaaataatta cctgcatcca cgaggtgcag gagcttcaat
    37801 gagctttggc gtacagcatt ttttcaacta catcagtcag gcttacaaac ccggtatgtc
    37861 aggaattaaa ttgaaaaaat acgatttgaa actcggactt tccctcattg accttggctt
    37921 cattagattt aatggtaatg caagaacttt taatataaga aatgcatcag cagtttggcc
    37981 aggatttgat actacaaaga ttaccggcta tgaacatgcc gattctattt ttagtgccaa
    38041 actttatggt gatgcaaact tgtcgcggag tggtagtgga tttaatatga tacttccagc
    38101 cgcagcatca tttcaggctg actggagtat tacgccaaaa tattatctca atctggcagt
    38161 cattcagaat attcagattt tcgatagaag tgtaacacgt gcttcacaag tttcgcttac
    38221 acctcgtttt gaaacaagaa gagtagaggt gagcttacca ttgtcattat atcagtacaa
    38281 ttttttcaga gcaggaattg cagttcgata taagtggttt gttctcggca gcgataacct
    38341 tggctggtgg ccgggtttgt atgaaatgaa tggccttgat ttttattttg gttttaaata
    38401 caccagtgac atttacgata acatccgatt taaaaaaggt aaaggtggtc attgtcctgc
    38461 ttacaagtga aagatgatga tcagccacgg ctgacagggg tgaaaggtga tgaagagaag
    38521 agtgatgaag taaattagat gattagataa tctgataatt tgataattca gagtatttgt
    38581 gtgtaccgct tacattttct taccgtttat attatcagat agaatttaaa aagataaaag
    38641 gagacagcag aacgttttct gctgaactcc tttaagggtg ggaataaaat ccccgttttt
    38701 tatagggtac gttgaccatc aacggtaacg gtattgccgc tgttgtctgt aaatgtgcca
    38761 tcaatctcat ctccggaaag tgttccaacc tttatattgt tatcatcata taaatcaccg
    38821 gatgatgtaa tagttccgga aacatcattg gttgatgtct gaccattttc ccgtgatatt
    38881 ccgccccatt gacctaaagg tcgcgagagt agaatattga acgttcctga ctcaggttct
    38941 gtagtagaat aggtgccttc aaaaccctca accaatgcat ttgaagtttc tttgataatg
    39001 gtaaatactg catttggatg acccggaatg tttgatgaaa ttatttgtgg agtacttccg
    39061 ttgttctgaa cagagaaagt gatgcttaca gcctgaccat ttaatgttcc tgtaaaaggt
    39121 gcaatgtaag gctgaccact aacccaactg actgtagagg ttagcgtagc agaaacgcca
    39181 tcaacaacca ttgttgcagt aatatcatta gtgccattct gaatggaaaa ctttatggtg
    39241 cctgatgatc caatgatgat gcctttgtaa acacctttgc tgcttccatc gtttgcagct
    39301 aatgcttctg cctgagttac acaactgctg catgtgtaat gagggttgtc tttgtctttc
    39361 tttttgcaag aagtgaatgc caatatggca atgcataaaa tgggaatgat taactttttc
    39421 atttatttaa tttttatttt gtgatttata aattattacc ggaaagagta tttgcctaac
    39481 caaatgtttt tataatcttc atcatgtcct atgtagaagc ggttgttgtc tttgtctgtc
    39541 agactagtga tactatgata aactaaatat tttccttttt caccaagctc ggtaaagttg
    39601 cttacagaat gattagacaa ggtaatattg gcaatgcgtg gaaagtaatg cgatgaaaga
    39661 gagtaattgc cactatatgc tgctgctact gatgcatagt atttagcagc ttgaacttct
    39721 aaaatttccc aatagacaga tttgccatca gcagacggaa cgaagttttg aattgctgaa
    39781 agaacttcat ttttactatc atctaacatt ttgtttacag catactgtgc tactagtttt
    39841 ccctgattgt caaattgcac acagacatag tcgtaatatc tgaattcata ggagttgccg
    39901 ccattcacta cttttttatc agacaactga cctgcaacaa gcacatcgcc tgaaggtgca
    39961 acaaaaagat tttcaataac tatcttctta ccggtataag gttgaccttt tttctgtgaa
    40021 ggaggcgtaa ccatttttgt tttaaaatct tttaccggtt cagtcgaagc aaatgcaagt
    40081 ttgccatctg agaatctcaa taaatgcaaa ttagcaaatt cagttcccat agcacgtttt
    40141 tcgtatttgt ccatttgctt gttgttggaa gtagaatatc cgggattgtc aattggtgca
    40201 tagctggtaa aaactttatt ataagcctca gtgcttttgt cagatccggc aatgaaatac
    40261 aagtctccat tagcttcgct ataatccatt atcaacatgt tgggcgatgg cgaagtaaac
    40321 tcactgcttt ttgcaacttt actgtctgca ctacattcaa tgtaataata tttcttggtg
    40381 tcgctcatgc cttcattagg tgcaaataca gcgaaaatat ttcctgattt cagtgtacca
    40441 caataaacta aggagtactt accttgtaat ggtagttttg tttcagtcat gtttaaacct
    40501 ttgtcaacgt gaaggcaaac gaactgatca tcttcaccac tatcataact tgcaagaata
    40561 agcacactac cgtcatcgtc attaccataa gccgcaaagc ctctgtactt gctatcgtta
    40621 ttctttggtt ttaccacttc tttagaaatg cggctgcccc attgatattt ttgtttatcg
    40681 aagttccagt ttctttccca gacttcctgt tgcagattca gtgacatact cataacatta
    40741 aatgaattgg taccaccaac aaaagctttt atcaaagtaa ctttttggtc gggttgtgtt
    40801 tgtttgtctt ctttagtttt ttctactcct ataaatttca gatttttatc aaagacataa
    40861 tcttcataat taacctgatc actctttttg tcaaccttca ttttgtaagt cattctgatg
    40921 ttgccgtcag cacttagttc ggcatttgcc agaatgcctt tttgggcagg ctttgacaat
    40981 tcgtgagttg tttcattgcc ttgtgcaatg agtagggtcg gggagaggat ggcagaagca
    41041 agccatactg cggtaacttt tggtagcatt tgtttcattt tgcgtttttt tatgaaacaa
    41101 aattagcccc tgtattatgg gtagtcaatc accatttagt agtaattatt ttttgaatac
    41161 tttgttaagt gcttcagttc ttgagtttac ctgaagtttg tgataaatgt tcctaatgtg
    41221 tgtgcgaaca gtttcagtac ttaaaaaaag ttggtcggca atttctttat atcgcatgcc
    41281 tttagaaagt aaatcaacaa tgagtcgctc acgctctgat aatttttcgt agttggggct
    41341 tacaggtgtt tccataaaac ttgaaactac tttacgtgca atatgactgt tcattggtga
    41401 accacctctg taaacatcaa ctattgaatc taataatttt gatggctgtg ttgttttagt
    41461 gagatagccc atggcaccgg ttttcagagc tttaaaaaca tgctcagaat catcgaatga
    41521 agtacatatg agaaaattgg ttgacgggca tttgtctttt aattcaagaa tacaatctat
    41581 gcctgtcttg cctcctaact gaatgtctgt cagcactaca tcaagttgca gcgtaggtat
    41641 gatatttata gcatcttcag catttgcaaa taaatgtttg caggagaaac cttcactgcc
    41701 atcaattaat atctgcagat tctctctgat gtgcttttcg tcttcaatga tacccacctg
    41761 aattgtcata aaacaaaaat aaacctttca ttagtgaatg caatactact aaagtagtat
    41821 attcagattt tttccaaaga aagtattgat ccttctcccg gtttagaaga tatttttaat
    41881 gatgctccaa tagctttact tctctcagtt atatttctca gcccgtttcc tgttgtcttt
    41941 tcattcaagt taaagcctat tccattttca gtaattatta gtttaaaaac gggttgaatt
    42001 tctatcctga ttgaaacctt agaggcattt gaatgcttta cgatgttttg taaggattct
    42061 ttaactatca tcagaatatt tctgctgatt tcaatactta ttttttctga aggtatattt
    42121 tcaggcaagt catattttac ttctatcgga aaatcttcca agtactctgt tgagaattca
    42181 cgtacacgtg caatcagatt gtctaatgtt gaattcatcg gattcatatt ccagatcata
    42241 tcacgcatgt tgtcaatcaa ttgatgcgaa gtgcctgtaa ttgtctgtaa agtttctttt
    42301 aactgctcct ggtctttggt tttcatttct aaaatttctg acaggaattt aatttttgtt
    42361 aatccggaac ccaattcatc atgaatgtcc tttgcaattc gcattcgttc attttcttct
    42421 gtttgtctga tagtgttttg ttgatttatc aactcagctt gtttgcgcca tcttagatag
    42481 aaatagaggg caatgatgat tgttgcaatg agtaaaaaca gaataataac taaagcattt
    42541 tgttttctga tattttcctt ttgctctttt attcgaagtt cattaattaa tgattgctga
    42601 ttgagaagtt tgatagttga ctctttcttt tcaaggctgt aacgcgcttc catttctgcc
    42661 aacctgttat taaatgcaat tggttgtatg ctgtctttga ttctgtaagc ctgaagtaga
    42721 ttttccattg caggttcata ctgctttatt gatgcatata gttcagaaag tgatttatag
    42781 caatcgcgtt caatagtttt gttattattg tcttttgcca gcaataatgc tttctgaatc
    42841 cattcaatgg ctttactttt ttgaccaagt tccacataga tatcaccaag ttgctgatag
    42901 gtgcctgcct ctatctcagg gtagtttccg ggatgaatga cctcaagagc gtgtagtgct
    42961 gtttcaagtg cttgtttggt cttgttatgt tccagataaa tgcgactaag gttagagtag
    43021 caaatagatt tgttgcgttc atcacctaag gttgttgcat acattagcgc ttgctgatag
    43081 caatttgcag cgctgtcatc gagcatctgt ttgtagtagg agttgccatt ctgaataagt
    43141 gaagatattt gtccggtcag actattcaaa ctggcaaatt gactgatagt tgtgtttatg
    43201 gtactaatag attgttcata aagattttgc ctgtaataaa ttttacctaa aactaaattc
    43261 agccaagctt ccccttcctt gctcgtattt tgctgatata ttttataagc tttgccataa
    43321 aggtcaagag cttttgaagg aataccttct ttgatgaaaa cttccgatag ttgaagacag
    43381 gaattaaaga gttgaataga agtgtcatgt gccgaatatg aacttatgct ttcagtaaaa
    43441 taaaattgag cagaatcaag tttgccaata taaaaataac aaatacctaa agttgatagt
    43501 agctgttgtt ttaattttaa attaatacca tttgatgatt gcagtaaatt agttaaagca
    43561 gcaattgcag gaatactttt atttgtttgt gattcaatta tactctgcaa gcatgagcta
    43621 gcaacaattc cttgcagata attgatttta actgcatgag attttgcttc taatgtaatt
    43681 acataagcat gcttaaagtc tcgtttgtaa atttcttcag cttctgatat tcttgtatca
    43741 atctgttttt gttgtgctgt aggagtagtg tctgatgcat ataatacaca gtgggaaaaa
    43801 aacaaacaaa cacaaaatat tattcttgac agaaaacgcg acattaaatt ggaaatcgca
    43861 tgctaagata ataaaacaat attactataa gcgagtaatc atgtcagcaa agaactttaa
    43921 attcttaaaa aatataattt acctgtttga ctgattagga atatcgtcag gatgcatttc
    43981 aaattgatct tctttttctt taaacttcat tatttgatca agtgcatctg gaataacatc
    44041 gctacatata acaatgaatg aacctttttt gccattctta attgcataat caatagcttc
    44101 cttttctaat ggaattactt taacaggctt tgttgggtca acagctttta ttccatcaag
    44161 cataaagtca attatttcct gttctgttct gcctcgtaga tttctgtctt gtctgatgat
    44221 aatatcatca aacattttag ctgcaagcgt tcctaatgca atgatgtctt catcgcgtct
    44281 gtcgccaaca cctgcaatga cacctacttt tggtttagca tcaacttttt cgaggaaacg
    44341 tgcaatagct tggaagcctg cagggttatg tgcatagtca accatgacat tgaatttctt
    44401 gaactgaaac atattcatcc tgcctggagt ttgaaccgga gaagggataa atgtttccaa
    44461 tgcaagacga atgtcttcaa ttttaaaatt gcggataaag gctgaaagaa ttgcaggtaa
    44521 tatattctga atcataaaca ctgctttgcc tgaaaaggtg agtggaatgt taatgacttt
    44581 atgtacgcga agtttccatg tgcctttgca aatagttaca aatccatttt caagaatggc
    44641 agcaagacct ccatcttcgc aatgacgttt tatcaacgga ttgttttcat cgaggctgaa
    44701 ataagccact ttgcaatcga gatttcgagc catgttagac actaattcat catcagcatt
    44761 taaaattgca taaccattgg gttgtacact ttcaggaacc actgctttaa ctttcgacat
    44821 ttcttcaagt gtgttgatgc ctttaagtcc aagatgatct gctgcaacgt tggttacaac
    44881 tgcaatatca caacgatgaa atccaagacc ggcacgcagt attccgcctc ttgcagtttc
    44941 taataccgca aaatcaacag taggatcttt caatacaaac tctgcacttt gtggacctgt
    45001 acaatcgcca cgctgcaaca tctgattttg aatatatatt ccatcagtag tggtaaatcc
    45061 aactttatgc ccacaggttt ttgcaatgtg agcaatcaaa cgtgttgttg ttgttttacc
    45121 atttgtacct gtgacagcaa taattggaat gcgataatta cttcccggag gataaagcat
    45181 atcaacaaca ggctctgcaa cattacgtgg cagtccatct gtaggcgcaa tgtgcattct
    45241 gaatccggga gcagcattaa cttccagtac ggcaccaccg gtttctgtca gtggttcact
    45301 gatgtcggga gtcataatgt caataccgca aatgtcgagt ccaataattc ttgcaatacg
    45361 ttcgcacata aaaacattgt atggatgcac aatgtcggta acatcggttg ctgttccacc
    45421 tgtactcagg tttgcagtac gtttcagaaa caactcttct ccttttttaa gaacgctttg
    45481 aagggtcagt tctttttctt ctaaaatatt ctgtgtcatc tcatcaattt ttattgatgt
    45541 cagcacattt tcatgtccat aacctctgcg tggatcagca tttacggcat caaccaactg
    45601 ttgtattgtg cttttgccat cgccaacaac tgcagcaggt ttgcgttgtg ctgcacaaat
    45661 aaatttgtag ttgatgacta aaatccgatg atcataacct gtaatatact tttctacaat
    45721 tacagaacgt gaaattttct tggctatttc taatgcctct actgcctgat cccaattgac
    45781 aatatttatt gttgctccac gaccatgatt accattcaat ggttttataa ctaaaggata
    45841 gcccaaatct tcaacagcat ctttcaagcc atcttcatca cgaattattt ttcctcttgg
    45901 tacaggaata gattgtgctt cgagcaggtt tttagtgtct tctttatcac aggcaatctc
    45961 tacagctatg ctgctggtag tgcttgcaat agtagcctgt attctccgtt gatttattcc
    46021 ataccctaat tgcactaagg agtgcctgtt caacctaatc catggaattc ctcttgaagc
    46081 tgcttcttca acaatggagc ctgtgcttgg gccaagacgt tcatcttcac gtatctcacg
    46141 caattgctga atgtcatttt gcaaatcata gcctgtgcca tcaattaatg cctgtgcaat
    46201 acgcacagag gcacgtgctg catacacacc tgctttttct tcaatgtagg agaacacaac
    46261 gtgatataca ccatgttcgc ctgtgccgcg cgtacggcca aagcctgtgt ccatgcctgc
    46321 aagtgtttgt agttcgaggg caatgtgctc aataacatgt cccatccagg tgccttcttc
    46381 aacacgttta aaaaatcctc cttcatggtc ttcgctgcat cggtgcgaat acatacttgg
    46441 aaacatgctt tttaatcttt ctaaaaagcc atcaattttg tcagtaggtc gctcttccat
    46501 ctcctgaagg tcgagtttca ttacaataag tttatgtctg cgtatgctcc agaagttggg
    46561 ccctctcatt gggcgaattt caagtatcct cataaaaaaa accttttggt tgtggcacga
    46621 atatagagac ttgatttgaa tattgaaaaa cttggtgaaa ctgcgaagag aaaaatagct
    46681 taattcaata cagaatagat ttagtgaatc gaagtattat tcaatttttg attcagatat
    46741 gcatctaaga aaaaagaggt tatctcaata tttgaaatag cctcttttta aggattattt
    46801 tacgctaatt aaggacaaac aacttctatt tgccatgcag taccactatt acaaccgcta
    46861 acataaattt caactaatct gctttttcca gggtcataat taaaagttac tgaatgagaa
    46921 ccagaaacaa aaccatcagg agatattgca cagttagggt atcctgaagg tgataggagt
    46981 gtgcctgttg aagctatcca ctgaccatca taacgaatgt cgagtctgtc agggatgcta
    47041 tacatgtcgt atgaaattgt tgctgttccg gctttgctgc caagattggc aaccaatgct
    47101 gttattccta cattacctga gaaactggcg ccacatccta atgggtcggt aacagaaaca
    47161 tcagtattgg ctattgtact gacattaccc gcacaatcac caacggcaaa ttttaatttg
    47221 taatccccat ttctcacgaa gttgggaata aggatacgta cataaggccg ttgagcaatt
    47281 gtgtcgaatt caattggtgc ggcccaatag gaatctgcac tgtcaatttg aacataaaaa
    47341 gtacagagat tagcattatt gcttgtgtta taattgaaat atagcaaaac accagcactt
    47401 acctgaacag catttggttt gtctgtaata gcaatggtac ctcctgtagc agtcggcata
    47461 ggtccttcat aaagttttcc gccactgcca actaccatgg ctttgttgat catgtctttt
    47521 tgatactcat tgacaccata atcaatatcg gtagattctt ttttacagga cccaattatt
    47581 aataataccg gtattgctgc aattaatatt ttattcagtc tattattcat tgttgtttaa
    47641 tttaaatagg ttatacataa tttcaagttg aagattataa cttttatagt tacttccttt
    47701 gtcgtaagct tttgcaaaaa tgtgattggt aagtcgcact gaaaaacgat cgcctagtgt
    47761 ggtgcccaat ccgagaatga aacctgcttt atgctcgttg aacatattct tatttaaatt
    47821 atcctgattg gtttctgtag tagagaagcc accatcatag gctcctgaac cactgatttc
    47881 tcgttttaat atttgtttac gcgttgcaga gataaaatgg tcgaaagatg ggccgatctc
    47941 tacaaaccat tttttcccat aacgtattaa aattggaata gaaatttggt tgattctgta
    48001 cttctctgta tagctcatct catcattaat gttttgtact gaattatcag aggagtagga
    48061 atataagtga gtaaatcccc tctttgaata aaatgcaccc attgaaacac tgaaaaacga
    48121 gagtaaatct tcttcaccca aaacaactct aaattgaccc ccggcatcaa agaaaactat
    48181 tggtttaatt ttcccggaaa tgtcagcgtc agagaagctt acttcatgaa actctttgtg
    48241 ctcgttaata aacttgccat agctaaatga ggcgcttcca cctaaaaaga cctctgcgtt
    48301 gtaacttggc ttaaactgcc ctaatctgat ttgtgcatgg atagctatcg gacagattaa
    48361 aatagctagc atgagtttgg tttttttctt catactttaa tgattttttt gtttagaagt
    48421 gcaatataga aattaaaagt tagtatccaa gtgtttctat tgatctgatt aattattttt
    48481 agaattacga cctttaacaa agcctgtagc catcactgct ataacagcga taaaaaattg
    48541 tgtaatttgt tttttcattt taattggttt tataattgta tgtttttgtt ttttaatatc
    48601 agtgttccta agcgatttgt aatttcttct gcacgctttt tttgttccgg agtaaatttc
    48661 gacctgtcag tcagaatttc agattcatgc tgcatttgaa gtaaatcatt ctgaagcacc
    48721 atgtagtcat caaatttcaa tccttctgtt ttactcttat tctctaattg tgtaactaaa
    48781 ttattcatgt agtctaagtc ggcatctact gtacttttct tgcacgaccc taacgtaaat
    48841 agcattgaaa atgcaagaca tataatttgg tatcgaacac tacaggccat tttagttatg
    48901 gattatttta ttgagttcgt gtattgttgt cctgcctctt caagacttaa agccttgcta
    48961 actttgcctg agttttttac taatttataa gaatgacttt tccatttgta aattgggaag
    49021 tcaaatccgg ggcctcctac acgaattgaa ggcatacttt cagatgattc atagagaagt
    49081 tctggaatac cgggcagtga aagtgtattc ttgaatttac cactctcatc tttcataaac
    49141 aaactaaaac cctgccctgt attgccatat aagaacgaac tgtaggtgat tacaaatata
    49201 tcttcggttt tgtcattgtt gatgtcaacc ggcactacct gaacctctac accttcgcct
    49261 tcagtcataa aagttttact tgctgcatca taggtcaatc ctgttaatgt actgatttgt
    49321 tttttatcat cactattaag ttttgatttc agaccggaga atagttgact tgcaacttct
    49381 gactcctgta cattattgtt ttcagattgt ttgcctgagt cttgctgtga gcaactgacg
    49441 attgtgaagc ataaaaatgc gaaggcagaa agtttgaaaa tattgaagct tttcattgct
    49501 aataagtttt atttaacaac tgtaatatta ttccgtaatg agaagtcata aagcttaagc
    49561 gattttttct gatcactgct tttgttataa taaaaagcat actcacctgc aggaacatca
    49621 ttttcaaagt aaactttata aagtctgttt gatacttttt cgattttaaa gtcaactaca
    49681 cctttggcaa aaccttcttc ttgtgaaaag gctgatttcc tagctatctg tatctgacga
    49741 ccgcttttag aagtggttgc ttttaccaag acaaattctt tagggctcgc tactccatca
    49801 taggtacttt ctttaacttc gttaatgtct tttccgtcaa tagaaagata gaaatagaat
    49861 acaggacgtt tatcttcaat tgtagtactt gaatgttcac cggccagtcc taccttggtg
    49921 cttatgccag atatgccacc tgaatagtga ttgcccattg actctccaaa tccacctttc
    49981 tttgtttgat tggtagtagt gggctctacc ataatatagt ccactttagg gctaaattgt
    50041 tcgtaataaa ttccgggtga tgtaagtttg gtaggtgctg gaatatttat tggatgatct
    50101 ttcatatcac cggaaacggt gttgcttgca gaactatttg tggatggatt catcatcact
    50161 tttacaaccg gatccggcac ccctgcattt tttaatgcaa taagcgattc ggtgctggtg
    50221 ttgaagttgc attttgaaag gttaattttt ttcagaataa ggtctttacc aactttgttg
    50281 gtgtgcattt taatcacatc ttcgttgttt aatgatggca tatttttact caccaacatc
    50341 atttcactga tgattttgtc agaaaccttt tgacctttta atccaataat tccatcggta
    50401 gaaaaatcaa aactgttggt tgaactttta attttgttaa tgataatagt gttgtctagt
    50461 ttggcactta ccattgaaac aatactttga ttgttgagtg attcttgggc atttgcatgt
    50521 tttattccct ggaacataac gaacgcacaa taaagaatgg agattaactt cttcatttga
    50581 tttttgatta tttaggccaa atgtagaatc acttttggcg gccaacaacc ttcttttatt
    50641 ggctgccggc tttcatttat tgaatgtgca ttttcattga ttatctgaaa ttactatttt
    50701 tgaaaaaaaa ccggctatga agcacttatg ggctattatt gtattcctct ctttggcatc
    50761 ttcacatgcc atgccggttc agcttgattc attatttatt aaggccaata attatcgaaa
    50821 tagtgatact gtcagagcta ctattcttaa tgacttagca attgaactcg ctgacagaga
    50881 ttatgaaatg ggattacttt attcagatag ttctataaaa ctctgtcatg atattaactc
    50941 ggacataaaa cttgctacag catattatgc aaaagccaga aatctgtcaa cagccggaga
    51001 agattcattg gctattattt cctatcaaaa gtcatattta ttaaacctga aagagaggag
    51061 attttccgca tgtgggacaa tgctgtttaa tatgggtatt tcatattata atatgtcaaa
    51121 ctacagtcag gctttggaaa agtatgaggc cgcattaaat attttcaaga aagtaaaatc
    51181 tgaaaaagga attgctgctt cactcaatag tttgggtatt gtagctatga acttgtctga
    51241 ctattcaaag gctatggaat attactttga atgtttaaaa atggctaaaa attcagacga
    51301 tagtgtttta atggctaatg catattccaa tattggaata gtttataaga atatagatga
    51361 tttgagtaaa gctttacagt atcaaaatct ggcattgcaa ataaatttga ttcgagagga
    51421 gcctttgctt atagctaagt gctataataa tattgcaatt gtttacagtg aaatgaatca
    51481 gcataataaa gcaattgaat tttatcagaa agcattagaa attaatcgtg aaataaagaa
    51541 taattttggt attgccagta attatataaa tattggaacc gaccttaatc atctcaatcg
    51601 gttttctgaa gcaaaccatt atatgctgct ggctgaagag ttgttcaata aaattggtga
    51661 tcagagctct gaagctatgg ccataaaagc aagaggtgaa ttaattctca atgcacctga
    51721 ttctgtacta aagtctttaa atataccaaa gacacagaaa tataccgtta gcctggaact
    51781 tctgaaaagg gcatcggcta tgtttgcaga aataggagat atatctaatc ggtcagatat
    51841 atgtcaggat ttaagcatga tttacgagta tcaaggtgat gctaaaaatt ctttagccta
    51901 ttataagcaa catatatcat tacgcgacag tgttatgctt gataataaga aagcagaaat
    51961 agagaggaca gagctaaaat tcaacttcga attaaaagag aaggagctaa atttgattaa
    52021 ttcaaaaata gcatctgacc ttcagttagc agagcaaaag agaaaatatc tgatgatggg
    52081 cagtgtcttg attttgttga ttacaggagg gggatttgtt tcctataaat taagaaagga
    52141 agcacagcaa aaaaggaagg acgcagagtt taaattggca aaccttaatg cagagttgaa
    52201 atcgctaagg ctgcaaatga atccacattt tatgtttaat tcattgaatt caattgctga
    52261 ttttattgat aagaatgagt cgcgaatagc ctctaattat actgttaaat ttgcaaaact
    52321 catgcggatg attttagaat attcagaaca gcaggagata actttagaag aagaattgaa
    52381 attgttggag ttatatcttt cgttagaggc attacgaatc aaccccaatc ttagctatgt
    52441 gatttctgta tcaccggaaa ttgatataag taatattctc atccctccaa tgatggttca
    52501 gccttttgtt gaaaatagta ttttacatgg tattattcca ttggcaaaac caggtgttat
    52561 caaaattgat gtttctgttt ctattgatga attgattgtt attgttgagg ataatggagt
    52621 tggaataaat agctcaatga aatctgacaa taaaaaatca atggcaatga aaattactaa
    52681 agacagaatt gcacttttta atgaacagca tagtgctgct gcatcagtta atttaattaa
    52741 tttaaatcaa ggtacaagaa tagaattgcg tttacctgta attcatagat tttaatagaa
    52801 tcgtaaatga taaaggcagt aattgtagat gatgaacagc attgtattaa cagaattact
    52861 acactgctta aagattatga aaatgaggtg cagttagttg caacagccag tactgttaag
    52921 gaaggtttga atattattaa aaaggttcag cctgatttgc tttttttaga tgttgaaatt
    52981 gcagataaaa ccggcttcga tttattaaga gaaatagagt ctccatcatt tgatgtaatt
    53041 tttacaacag cacatgataa atatgctgtt atggcattta aattttgtgc aattgattat
    53101 ttgctgaaac ctgttgatga gagcgatttt gcaatcgcca tgaagaagat tcttgatagc
    53161 gatgaccggc aggatatgac aaggcaactg tcagagttga ttaaaggagc aggactaaat
    53221 aaaccgagaa aaattggtgt acccacattg aatggaattg tgtttgtttt gatttctgat
    53281 ataagattat gtcgtgcaga tggaaattat actgagattt ttttaaataa caatcacaaa
    53341 attgttgttt caaaaacatt aaaagagttt gatgatttac tttcagatta ccagtttttc
    53401 agagtacata actcaaatct agtaaactta gactatgtta aaagttataa taaaggtaaa
    53461 ggcggttttc tgattcttga tgatgacact aatgttgagg tgtcatcacg cagaaaggac
    53521 gaactgctaa aaaagttaag cctgatataa atcaattaca tctgaaaagt tttgtttaca
    53581 gaaaattaat tctgttcata aacacttctt tgtaactctt gccaatagga atggttttgt
    53641 cattgataac tactgcacct tcatccatgt gacttatttt atctaaggca ataatgtagg
    53701 agcggtgtac gcgcatgaat ttttcgggtg gaagtttggt ttcaatatct ttcattgtgg
    53761 catgaatcac aaactgatct tctttagtct gaataattac ataattgtcc attgcctgca
    53821 gccagtaaat gttttcaaga aatactttta caagcattgc acctcttttt acaaagaatg
    53881 agttttgaat aacctgattc tctagagggt ttgaaggtgc ttcaacaagt ttttcttctg
    53941 taggttttat tacaggctgt gcaacagatt tctctgtctt agatgaacca atgacaacag
    54001 catagcctag taattggttt ttttcatcac gaatagggga gcatgtaaag tcagctttta
    54061 tattactgta atctctaaca atagttatat tactctgctt aatacttaaa acctcatcct
    54121 gtttcatgag tttacctaag ttgtcggcat tgacaatttc gtattcatca ccttcaattt
    54181 ttataagttt aaacaaactc atgccttgcg cactttcatt gccgaagcct gtcatgcttt
    54241 ctgcttttgg atttaaaaat gtaatacgga actctgtatc ggcaaccaaa atggcttctt
    54301 ctacatttgc aagagcattg gcaaagcgtc tttcattttc acgcacctgc aaatctttct
    54361 gaaatttata caatgctatt tcaacagttg tttgcaagtc agattcgttg aatggtttta
    54421 ccaaatagcc ataaggctca gtgattttag ctctgtccag cgtgtttttg tcaacaaaag
    54481 aagtgagaaa tattacagga atatcatact tctgttttat gtgttctgca atgtgaattc
    54541 catccaaatc gccttttaac tttatgtcaa gaaaaaccaa atcgggttcg tgttcttcaa
    54601 gtcttgcata ggcattttgt gccgatgatg ccgtggccgg aacatcatat ccaagacgaa
    54661 ttaatgtttg ctgtatgtct tttgcaacga tactctcgtc ttcaacaact aaaattctta
    54721 ttttgtccat aattgaaaaa tttaatacga tggtttaaat ttaattatca cttttaatcc
    54781 gttactgttt tctactgtta cttttccgtc aatctgatcg ctcaacacat tcactaattg
    54841 aatacctaat gtgtctgcag tttcaaaatc ttttccatca ggtaatcctt ttccattgtc
    54901 agcaatactt agcagataat ggtcatctac ctttttaaat gtcacccaaa ttgttccccc
    54961 atcatctgca aaagcatgtt taatagcatt gcttacaagt tcattaatta tcaatccaca
    55021 tggtactgct gtatcaatgt ccaaagatat tttttctgcc tcaatctgca aacgtatttt
    55081 ttgaggattg gcattttgag attgcataat ggtttcgcaa aggcgtttga tgtagtcatc
    55141 aaaatcaatt cgtgatgttc cgtcactctg ataaaagcga tcatgtatca aagccataga
    55201 acgtattctg tcttgtcctt ctttgaggat tttatgcgat gccttatcct gcaaataagc
    55261 actttgtaag ttgaacaggc ttacaataat ctgcaaattg ttttttaccc tgtgatgtat
    55321 ttcttttaac agaatattct tttcgtctaa cgattcacga atttgctcct ccatcttttt
    55381 atgcagggtg atgtctgtgt ctattaccac aatattgctc aattcatctt tgtcgttaaa
    55441 taccggtgtc agcgtactgg aagtccaaag ttgttgtccg ttgcgatgtg taattttagt
    55501 ttcataaata actgacttat gctcctcaag gcagtcttta atgattgttt ttatattttc
    55561 attatacgaa agctgtgtta gcttactgcc ttgcttacgt atgatttcat ccatagtgta
    55621 gccatgcatg cgtgtataac cttcattcac aaattcaaga ttcaggttat ggtcaaaaat
    55681 cataacagca ttgtctgttt cgctggcaac tattgaaagt ttcgcaagtt gcatattgtt
    55741 ttccctaagc tgatgggtac gctcattgat tttctgtctc aatagtagtt gtgcattccg
    55801 taaacttctt gttcttaatt tcagaataac taaaatgagt aatgttaatg caacgaagta
    55861 gataaaataa aaaaagtttg tccgccaaac aggtgatttt actttaaagt cgtaggtaag
    55921 tgatggactc cagtcatgac cgttggctgt tgcctgcacc tgaaatgtat agtttccggg
    55981 tggcaggtta gagtaatttg caatgtttaa gttggtagca ggcacccagt agtcatcaaa
    56041 gccgtcaagt ttccatctgt attgtaaagc atccggtgat gcaaaataaa ttcctgtaaa
    56101 atgaaaatta agattgttgt agccatacgg aaaaaccgga ttcacaggta agccagtaat
    56161 gctgtcaata gaaaaacctt gttctgacca atttacctcg cgcatgtagg ctttgatgga
    56221 ttgcagcaga actactggag ttgtactttt actgtaaccg gaatttggat tgtatcgaac
    56281 agcaccgttg acagtagcaa accaaagaaa gccatcttta tcgtaagtag ctgcattggt
    56341 ttcaacacca taaaatccat tacttttatc aaagtgaatg atgtttgtga aatctctttt
    56401 cagataagtg ttccagtcaa ttctgtcaat gccaagtgga gtacccatcc acaaataatt
    56461 gttgtcgctt gcaacagtta aaattctatt gttgaacaaa ccatttccaa cttcaagatg
    56521 tctgatggca gttgttcctt tcttttttga aaaaatatag acaccaaaat caaaagttga
    56581 aaagataata tttccttcta tatcttgtgt gattgaagtg ataggtgttt tattaagatt
    56641 caacttgtcg ttaagtgaaa ctgctgtttc attatgcata aagtacatgc cttgcaaagt
    56701 gccaacccaa accaagcctt gtctgtcctg aaataaatag cgaatgttgt cattctgtaa
    56761 gccattgagc ttatttaaaa cgcgcagatt gttgttttcg taaatataaa taccacggtc
    56821 ggtgccaaac cataaactac cgttacggtg tgccataaca gcataaataa ttttattgtt
    56881 aagtaaatca aatggaaaag aaattttaga taagtcagtt gaaactaaag ccggaccggt
    56941 tgatgttcca aaccagaaat tttgtgctgc atcctgtgca atactccaaa ttgtggcacg
    57001 tgcaagaata ttttcaaaag gaggtgtata gactgtattt ccatgaatgt aattcaatcc
    57061 gccatttacg atgccaaacc aaattctgtt taagtgatcg gtgtatgcag taagtagtct
    57121 gttgtcatta aatccgtttt ctgttgtgta ggtgataaag cgtccacctg aataacgaca
    57181 caaaccacga ggtgttccaa tccaaatgtt tccttcatta tcttctttca tgcacaaaat
    57241 attgttatct gtgagaccct catcttttgt caacgtgaaa aaatgaccat gatctatcag
    57301 gcacatgcca ttaccataag tactgaacca tttattcttc ttataatctt caaggatggc
    57361 agtaactttt ttaactgaaa cacctttgtt gatatcaaat gcgccaaact ttccattttg
    57421 atatatgctg atgccttcat tagttcctgc ccaaatgctg ccatcattca attcagaaat
    57481 ctgataaata atatttcctg taagtccttc gttgatggta aagtttgtaa actgtttacc
    57541 atcgtacttc gacaatccgt tagtagttcc aatccagatg ttacctttac tgtcagtcag
    57601 cagacaggtg acttcattac tcaccaaacc tttcagaata gtgtaacttg aaaaacgtgt
    57661 tccgtcaaaa ctgttcagtc cctctgatgt accaatccaa tatcgtcctg ttttgtcttg
    57721 cgtaatggct gtggcattgt tgctgagcag tccattgtca atggtgtaga atttttttct
    57781 gataccatta aagacaccaa tgccaccacg tgttattgcc atccaaagat ttcctttatt
    57841 gtcttcgtaa aatgctttta caggattgaa agatgcaggg ttgtcttgtt cgaaacgata
    57901 aaaattagtt ccatcaaacc agcaagcacc attcatggtt cctatccaaa gctttcctgc
    57961 tttatcctga tgaacggcat taacttgatt gctgggcatt ccattggcaa tagtgtaggt
    58021 agtgaagtta taatgctgac cttgcaccac tgaactgaac agcataagca gtgccataaa
    58081 aaacataggc atttgtaaga tgctttggtt tcttattctc agactacttt tatccttcac
    58141 ttgcatttat aatttttagc ttttgctttg gaatgaatca ctttatttaa agtagataat
    58201 cttcaggctt cagccaatca aatccgtttt cagctatatg tttcattctg ttaaagtaat
    58261 ttaactgtac atgattgagt ttggttacat cccatgcttt aaataaatag gcatagacaa
    58321 aaatacctcg taagtgcagt aattccggca tcaaattaag ctgattgagg tcaaagcgat
    58381 tgtatttttg atagcctctc ataaaactat ccataaaata gcgtgcccag cctctgcggt
    58441 taaaatcatt tacaccattg taattaactg caaaaaacaa cggacaggaa atgtcattta
    58501 caaaccaagt ataacatgca tcttcacagt cgaacaatgt caggcgaccg ttacattcca
    58561 taaaattacc tctgtgaata tcgttatgca ctacaccaaa tgaatctgat gtggaaggca
    58621 gttgtttaac tatattaaga agttgtgtcc aatattccca tgatttcatc tctgattttg
    58681 gcaaataagt ctcaaccatc ttatttgatt tttctggcca gatagtcctt ttcttaatag
    58741 tgtcaggagg tgaatatgtt tttgaacact tatgaatcat tcctgtaatt ctgcccattt
    58801 gtgcaatggt attactgttc cagttttttt cgcttaattc aacgcctggc gctgctgtaa
    58861 acttcactac actgaagtaa gaatcgcctg ttgttacttt ctcgcagaga ttgttattta
    58921 cagaaggtaa tgggtttgat acaggaattt tattgtcatg caaaaagttt atccagtata
    58981 tttctgagtt cactgtatca aaacactgat gtgatgaatg tgtcagccgg agaataaatt
    59041 ttttatcacc ttcactgaaa cgataaatga tgttcacatt atcacgaagt aaagtgagat
    59101 cggaaaactt aatgttataa agttttgcag cttcagaaac agcctcgtta gagagtgctg
    59161 cacgtacaaa ttcgttcatt ttttaatggt tgacaatggt gttgtaaagt tgttgaagtt
    59221 gtagtgcgca gggtttaata tcaaaatttt caattacatg ttgtcttgca gcagttgcaa
    59281 attgctgata agtgttgttg ttcatattgt aaaaagtgcg gatagaaccg gctaatgctt
    59341 ctgcatcttt ttcaggcgta agaagtcctg ttttgccatg tataacctct tcgggaatat
    59401 cgcagtgtgt ggttgaaatt accggcatgc ctgtgcactg tgcatcgagt agaacaattg
    59461 gtgcaccacc ttcgcagtca cgcgtgtctg aatagcaact tggatgaata aaaacatggt
    59521 attgtccgaa gaattcatac agctttgtaa actcaataaa tccgataaag cggactttat
    59581 gttcaatatt atgttgtttt accaatgctt caagtttgcc tttaatattt tccaattcat
    59641 tgccaacaaa agtcagtgtc atattcgggc attcatttaa tgcctgaata aaagcctgaa
    59701 gtgtataaat atgtcctttt ttctgactgt agttagacag ttgcagcaga tgcagttcac
    59761 cttcgttttt agttcttgct acagcaggaa ttttagttgt ctccacacca aggtgcacaa
    59821 catgaacttt tgcagggtca caaccctgat tgactaagat ggaagcaccg aaagcacctt
    59881 cacaaataaa gccatcggcc tgttcatata aagattgata acgctttttc cattcctgaa
    59941 aacgaaaggg caaactttca taatcaaaac cataaaaaga aacaaaatag ggaaggcctg
    60001 taattttttt tagttcaaga tattgccaac ccatattggc aaagtgtgtg tgaatgagtt
    60061 caacgccatt ttcattagcc catttaccga tatattcaaa ataatgtttg ttttttctga
    60121 gaatggcaat acggttaata atttttgaga aaatattttt ggcatgatcg cgcccttcca
    60181 acaaatccgg actaacatat tccggcatgg gtaataaatt tatttttcgt gtaacaaatt
    60241 gttcattgta atagcgatat gctgcaacat tcacctctgt atcagaaaga ttgttcaaca
    60301 tcctgaacac ccagttctgt gcattaggta aatagctttc aaaaagttgc agtgttttta
    60361 ctgccatgaa atatatagtt tttatttcac gctaaatacg acatttccat ctgtaatttg
    60421 catggttttc ataaatcttt attaaaaact tgctgttgaa accgtaataa aaatgtaaat
    60481 gattaaattt gctaagaatt tagaaccatt atgagcaatt ttgttgtttc agcacgcaaa
    60541 tacagacctt catctttcga cacagtagtt gggcaaggac atattaccac aaccctaaaa
    60601 aatgcaataa aaagcaacca tcttgctcag gcttttttgt tttgtggacc aaggggtgta
    60661 ggtaaaacaa catgtgcacg tattcttgcc aaaaccatta attgcagcaa catcactgct
    60721 gatacagaag cttgtaatga atgtgagtca tgcgtttcgt ttaacaacgg acagtcgctc
    60781 aatatttttg agcttgatgc tgccagtaat aactctgtgg aggatatccg cagtttagta
    60841 gatcaggtgc gctttgcacc tgcaagtggt cattataaaa tttatattat tgatgaggtt
    60901 cacatgcttt cttcgggtgc attcaatgca ttcttaaaaa cattggaaga gccaccacct
    60961 tatgctattt ttattttagc aactacagag aagcataaaa ttattccaac tattttatcg
    61021 cgttgtcaga tttttgattt caatcgaata aaggttgaag atatttcaaa acacctgcag
    61081 caaattgctg taaaagaaaa tattgaagct gaccctgaag ctttgcacat tattgcacag
    61141 aaggccgaag gcgcattgcg cgatgccttg tccattttcg atcagatggt tactttttcg
    61201 ggaaacaagg ttacctacaa agatgtcatc aacaacctta acgtcctcga ctatgaatat
    61261 tatttccgca taaccgatca gattttaccc ggaaatattt catctgtgtt attaactttt
    61321 aatgagttgt tgaatgaagg ttttgacgga catcatttta ttgccggact gtcaggacat
    61381 tttcgtgatt tgatggttgc caaagatgaa attactttgc agctattaga agtaacagaa
    61441 gtagtacgcg aaaaatataa agttcaaagc aaggccgttg aattatccgt tttattaaag
    61501 ctgattgata tttgtaatgc atgtgattta tcatataaga gtgtccgcaa tcagaggctg
    61561 catgtagaaa ttacattgat gaaaatgtgt gccttgtcag gaaaactaac ggtgaatgaa
    61621 aatgccatgg ctgctaaacc cgtagtggtt gaaaaagttg ttgcagagaa aacaaatgta
    61681 gctacaccgg ctgcgcaggc accaaaaagt gaacctgctg tagtttctgt taaacaggag
    61741 agtataaaac cggaagttgt taaacctgaa acaaaaataa atgaaccagc caagcctgaa
    61801 gtgaaagagg cacctacagt aacaaaagca gaagagccta tagccacaat ttcattatca
    61861 ggactcaatg caaaaaaaac aacacctgat aaagcagaac ctacagatgt gaaatctgaa
    61921 aaaaaaaatg acagaccgat agttgttgat gagttaaatc ggatatgggc tttatatgca
    61981 gatttgcaag acaataatgg caacaccaat ctttcaaaag cattgctcag aacagagcct
    62041 gaagtgatta atgaaacaga aatacatgtt actgttgaca accaagtgct acgggataat
    62101 ttattgtcgt tgaaaagtga atttcttgaa tatttcaaaa aagaaatgca gaacgatttt
    62161 ctgactttgg aagttcgtgt tgaagcatca gagaaagttc ttgataaacc ctatactgca
    62221 agagagaaat ttaagaagat ggctgaaaaa aatcctgagc ttgaaaattt cagaaagcag
    62281 tttgacctta atttcgatta gtcaaaaata aatttcaaag ttgttttaga aaagtaaata
    62341 atgataatag atcccacgca gataaaaaca ggtgagtttc atgcattgat gcttggtgct
    62401 gttgcaccac gtcccattgc ctttgccagt acggttgata aagatggaaa gccaaatctg
    62461 agccctttta gtttttataa tgcctttggc agtaagccgc caatacttgt tttcagtcct
    62521 gcacgaagag tgcgcgacaa taccataaaa cacactttgc aaaacgtttt tgaaacacgc
    62581 gaagtggtca ttggtgctgt aaattataac attgtccatc aggcatcatt agcaagttgc
    62641 gaatttccgg aagatgtaaa tgagtttgaa aaagcaggat tcactgctgt taaagcagaa
    62701 aaggtaaaac cttttctgat aaaagagtcg cctgtaaatt ttgagtgtaa agttttagat
    62761 gttattgaaa caggtaaatt aggtggtgct gctaatttaa tcatttgcga aatactgctc
    62821 atgcacatac aggataatgt gttgaatgag caaggtaaaa ttgatcctga taaaattgat
    62881 ctggtagcac gcatgggggg cgacttttat tgccgtgcat ccggtagcgc tgtttttacc
    62941 gtgcctaagc ccaatatgaa actgggagta ggttttgata atattcctga acatattaag
    63001 aaaagtgaag tgctaacggg taacgacttg gcgcagttag ccaatcagga aactattcct
    63061 tctacagaag aggtagcgca ttttaatcaa tctgatgaaa tgaaaaaatt taacgaagcg
    63121 cttgccaatg acccattgca tgcacagttg gccaaacatc ttgctgcacg caaattgata
    63181 catgcaggaa aagttaaaga agcttggatg ttgttgctgg cataataaaa cgcagcaatt
    63241 aagtgataga ggcactttaa tagcaaagtt taaaatttct tgaatctgaa gttgctgtgt
    63301 tttaaagtaa gctagtgttc aaataaatag ccacaaaaac attcgttttt aaaaactaaa
    63361 cacttacttt tacagcacta aataacaatg ccatgttaga aattagactt tgtttgcttt
    63421 tatccgggag tatgctgcgc ttttcttaac cgcagccaca acagaatttt ttttacattt
    63481 ttagttttac aaatcactaa ttaacgaaac tattcaaaag tctgcaattg ggtaaaaggc
    63541 aaagtgctgc acctgttttg gatttaagaa taaaaagaaa agtaaaatga aaaaactcat
    63601 actattaatt gccgtctgtt gtattttcac tcaaacaaca atagcacaaa ttaaccttgt
    63661 acccaatccg agttttgagg atacggtagc ttgtcctaca tttttaactc aaatagacag
    63721 agcaactggg tggatgagtt ttagttatac accagattat tttaatgctt gtgcatcttc
    63781 taatgtacca gtttcagttt ctgtgcctca taatatttgg ggtgatcaga ctgcacatac
    63841 aggaaatgct tatgctggat tcggggctta tagtgttggc tcgttattaa gcattagaga
    63901 atatgtttgt acacaactaa ttcagcctac agttccactt caaaaatatt atgtttcttt
    63961 ttatgtttct agttcttttg cttatactaa tggatttaac cctggtgtag cttgtaataa
    64021 tattggaatg aaattaacaa cacattccta ttctccatca acccctttaa ttcctgataa
    64081 tttagctcag gttgtgaatc atagtattgt taatgacacc attaactggg ttaaaatttc
    64141 cggatcattt attgcagatt ctgcctatga ttttttatgt attggaaatt tctttgttga
    64201 ttctttaact aattacgttt taataggagc aaattctggt gatgcttatt attatttaga
    64261 tgatgtaaaa ctatcgactg attctgcttt tgtaaatagc attgtcgaaa caaaagacaa
    64321 tggttatatt aaaatatttc caaaccctgc aagagactgg atagttgtag aaggaaggaa
    64381 tcttaaatca attgaagtgt ttgatttatg cggaagattg cttattccaa aagtgcttgt
    64441 ttcttccttt aaaacagaag taaatatttc ctcttttagt tctggcattt ttctatttaa
    64501 gatttacact tcaaccggta tttacataaa taaattttcc cacttttaaa ttgaaaatta
    64561 tgaaaaataa attcaaatat ataattgtat tgagtctgat attatgtaat gtaagtttaa
    64621 tattcgctca atcaacctct gcaacattta atgtaataat tcctaatcgg cccaacatag
    64681 caggtaacga ttatgttggt tgggacaata cggtaaatgt gccccttgag attcgacata
    64741 atgcaaacca atcaataata ttctatacaa ccggaataca acgcgccacc atactaggca
    64801 acggcagagt aggaatagga atcaataatc cgttgtggca attagatgta gccgatgaga
    64861 ttaacatatc tcagccacgc aactattata tgataggtgg cgaaacagta cttcataatg
    64921 ccggaacaga aaatattttt accggtgtaa attgtggaaa agatattttg gctggaagtg
    64981 cctcgggaaa catgaatgta ttcaatggat attctgccgg tgaatttgct tccggcaacg
    65041 acaatgtttt tataggtcat agtacaggat gatatagtaa aggccgatcc aatatattta
    65101 taggctcttc tgccggtatg gacaatcttg gtgattataa ttcaatagtt ggttttacag
    65161 ctggaatgta tttaactaca ggaattgaaa atacatttat gggattacgc tctggtgaat
    65221 gtaatacaac aggaagttat aacacctttt atggttcaag agcaggcatt ggtcaaaatc
    65281 catcaacacc aattgttggc cgcaacaatt ctttttttgg ttatgccagt ggccaggcaa
    65341 ttaaagatgg taatgataat atttttgccg gaagtcattc tggaaatagc aatagttcag
    65401 gttcatataa tactttcatt ggtggaaatg caggatataa taacgaaaaa ggaacttata
    65461 atacttatat tggaaataat gcagggtcgc ctgtaagtta taatcttcat tatgcatcag
    65521 caataggcac caatgccacc gttatgaatg ataaagtaat gatattggga gataatgata
    65581 taaacgtggg cataggcatg tcaggtgttc taggcggccc tcttaccaaa ctacaaattt
    65641 taagtaaaaa tgtaccacag tttcgtattt cttatgatta tggaaataat atacacaccg
    65701 actttcaaac aacagcaagt ggtgatttga aaatcaatcc aacaggacac tttacaggat
    65761 ttgatttgtc tgtttcgcct gcaaatgttg ttgatattaa ttcttcctca tcagcaatta
    65821 gcggattgcg tttaagaaat ttaagtacaa caagcaattc aaatggtaaa gtacttagtg
    65881 ttgacaatag tggcgatgtt attttggtga gggataacag tgtagaacta attgctatga
    65941 taaaacaact tcaacaacaa gtaaattcat tacaacaaaa attagaagca acattaaatc
    66001 aacactaata attatccaga aatcggattg actttaacca tacacattac aataaatttt
    66061 taaatttcct tttctggtta gctttgatgc taacaatata caggcaattt tcaatcaatg
    66121 atatttagag tgcccctaaa tccccaaatg gggacttctc accatacata ataaaactaa
    66181 ggagataaaa gattatgcaa tgttttatgg tgcaacacca ccattagctg gaacatacta
    66241 ccacaacagg aaccattaaa tgccatgttg attttttaca attccgatgg cagcatttta
    66301 aagatggcta agattcagca aataggagaa ggctctctgt tggtatatgg tagcaacctt
    66361 tcatcaggtc agtatagtta ttctttagtg gtcaataata caaccattaa aaccaaacaa
    66421 atggtaaagg taaaataggg cattttaaaa acaaaggagg cagctgcatt cgcagccgcc
    66481 tcctttgttt tattgcataa tcagcttatt tcacattcaa cactttaaat tctgtacgtc
    66541 tgttttgctg atgttcttct tctgtacaag gcacaacacg tgtcccttca cattcgcaat
    66601 gattaatcag cttagattct ccatagcctt tgtaagtcat ccttgacgct tcaacacctt
    66661 ttttaacgat atattgtaca gcagacttag cacgtttttc agaaagatcc atgttgtact
    66721 tgaagctagc acggcagtct gtatgtgagc ctaattctat ctgcatctta ggatactttt
    66781 tcataatgtc aaccaactta tccaattctt ttgcagcatc aggtctgata ttccacttag
    66841 ccaagtcata ataaatattg tccacacgaa ttacatcacc ctgacagaag aaaccaaagt
    66901 ctgctttcag aacagttgat tttgttaatc caatagtaga gcggtcagca aagttgtctg
    66961 cacaattttc tctaaaggtc atcactttgt agttagtctc aggctctaaa tcaaaatgat
    67021 aatttccatt tgcatcagtg agtgtttcag tttctttatt tgtttttaaa ttcatcagtt
    67081 taaccgggtg gttaggtaat gctgatttgt cttcttcaag ataaacatga ccttcaagag
    67141 taaatgcagg gatttttgat aatggaattt taattacctg cggctcgtca tttacgccat
    67201 tgatagatac tttattttct ttgtattcat ccttcgatgc aatgaaatcg taattctgtc
    67261 cgattgatag acatgatttg aattttcctt tgtcatcagt ttgtacttca gcaaacttat
    67321 tacctccttc attgattaca actttagcat tggcaagtgg caggttggtt tctttatcat
    67381 acaccaaacc atccaaaggc acacagttct ttttaaacat gtacaaatcg tcattctcat
    67441 ttttagtgtc acggtttgat gtcaagtagc cacacatgtt tttcttatcg taaacataag
    67501 caaagtcatc aaaggtagaa ttgagtgggc tgccgatatt ctctgcagtt ttgaattttc
    67561 cattctcaaa ctcacattta aaaatatcta atccacccat accactgcga ccatttgaag
    67621 agaaaaacag attgccctca tgatcaatat aaggaaatac ttcattttct gtagtgttta
    67681 cttcggcacc aagatttaca ggtttgtccc agtcaccatt tactctgttg cagacccaaa
    67741 tatccatgcc gccttttcca ccgggcatat cactggcaaa gtaaagtttg ttgccatctt
    67801 tagaaataaa tggatgagca catgagtagg tcttgttgtt gtatttaaat tctttaaatg
    67861 catcaaattt tccattaacc tttgttgcac ttagtatttt tagtcttact attctgtttt
    67921 gatcacgtac aatttttcca ttctcttcat agtttcgggt aatgtacatt tcgttgccgt
    67981 cagaagaaaa tgaaacgggc ccatcattat attttgattt gtcggcaagt tctaataaga
    68041 ccggagtgct gaaattattt tcttttccct tactaaacca taaagacaga tatggttttg
    68101 atgtccaatc ctgtgttttg cttttaccat cttcacgcga tgatgcaaag acaattccat
    68161 cttcataagg cacagcacac atgtcaccat taacagagtt tatcagtaac cgtttaacaa
    68221 tccatgatcc ctgctgctga ccaaaggtgt tgttctttaa agcatttagc tgctctgcca
    68281 ccaatttatc attcgggttt tttgctaggt atttttcaag atgcatttcg gctttgtctt
    68341 ttttatcaag acttatcagc actttagcat attgcagatg ctcattgttg gttgcattgg
    68401 tatctgctgt aattttctca taccactttt ctgcttcttt atattgattg ttttttctgt
    68461 agcagtcggc aagttttgat gccgtaccat gattgtattc tttagagaga actttctcat
    68521 acagcggtat tgcttttcca aagctgtact tggaataaag ttggtctgca agaaccaaat
    68581 catcctgctg tgcaaagctt gccgttgcaa ctaaaagtgc tgcaaaaaca aaaactattt
    68641 tctttatatg cgatattatc ataatacagt ttttaaattg gttagaagaa acgtggacta
    68701 agtacacctg tacgtttaag cggaatgtcg tagccaagtg ttatctcatg tgtgcccgag
    68761 ttgtatttgt ttaattggtt tgttgtgtaa tcataagaat atccaaggcg caattgtttg
    68821 gttaaattga cttgtgcaaa aaccgatgca gcggcacctg ttctgtatgt taatccaatc
    68881 cagaaaattt cattgaagta tgctgccaaa gttacatcag cctgaatggt tgcaccatca
    68941 gatgctttta tcatggtcat tggcttcaat tttatttccg gactcacatc aaatacataa
    69001 ccaccggtaa ggaagtaatg aaagttctga tgtgtaaatt tatttttaat atctcctcca
    69061 ttcactgagt caatgtagtt ttcaatcaac cttggaatag aaaaacctgc ataaaaccgc
    69121 ttggtgttat aatatattcc gaatcctgca ttaggcacca acttcgaaac attctgactg
    69181 aactgaatat cgttcgggtc gtgtgtaaca acatcaagat agttttcttt catgtggata
    69241 agcccggcct gcaacccaaa ggctaaacgg ccattctcac tcgtttgtaa tctatatgca
    69301 tagttggtaa agaaacctac tttcttttct acaccgatag attcattcat gaaagaaatt
    69361 cctaagccca cttttttatt tttcacagga ccatgtagtg aaacagtctg tgtttttgga
    69421 gctccatcca taccaaccca ttggtttctg tacaatgcag ttgccgatat ctgatcgcgc
    69481 gagcctgcat aagccgggtt gataaacatt tcgttaaaca tgtattgtgt aaacaatgga
    69541 tcgtactgtg cattagcttt aatgctgcag atagaggtca gtaatactga cataatccat
    69601 atttttttta ttgttgaata cattgctata ctgattttgt tgttgtgaat tctgtttatt
    69661 gcttctttca tattcttatc gttttagtgt cagatatccc tgcattggct tcttgccatc
    69721 attcaggtcg agtatgaaat agtatgttcc gtttggtaat tgctgtccga gataaacacc
    69781 ggcaacgttt gaagtaccgt cccacgaatt gtcgtagtct cgttttttat aaaccaagtt
    69841 accccatctg ttgtagatat agagtaagtt gccaggatat ttgctcaagc catcaatcac
    69901 ataagtgtcg tttactgcat taccatccgg tgagaatcct gtaggcacaa acagtgtgtc
    69961 acagataaca cttaccatta cttcgctcac cacttcgcaa ccttcgttag agcgaatggt
    70021 tacagtatag gttgttgaag cttttggtgt tgcaatagga ttggcaattg tagagtcatt
    70081 taaacctgct cccggatgcc agctatacca tgaaccaccg gttgcaaata gttgtgctat
    70141 ctctccatta ttcggacaaa tcatcatgcc tggacttaca gtgtaaccac tcactgaatt
    70201 tactttcact tcaacaggtg tagaagtaaa gttgccacaa gagttggtta acgatactgt
    70261 ataaacacct gcagcaggag tagtgagtga tggtgatgtt gcaccgggca atacttgtcc
    70321 attaacatac cattgataag taccattctg aactactgtt gcatttaaag ttacttttcc
    70381 gccaacacaa aactctgtcg gtccgtcagc attcatatta aatactgaga tgtcggttac
    70441 tacctgtgtc tgttgtacag tacaaccatt agcatctgta atgctaacag tatatgttcc
    70501 tgcaccaaca ttggtaaggc tttggtaagt tgaaccatta ttccaaacat aagcataagg
    70561 ttgagtacca ccggctactg taaggtctat gttagcacca ttacctgtaa gacagttagc
    70621 ttcattagct gttgaagatg cagtgagtgc aaatgcaggt tgtgtaattg ttacagtctg
    70681 ctgatctgta catcctttct gatctgtaac agttaatgaa tatgttccgg gagtaaggtt
    70741 gctcaattga gaactgcttg aaccgttgtt ccactgataa gtgtaagcag gagtaccgcc
    70801 ttgcgttgtt acgctgatac taccattgtt cagtccatag catggtatct gtgttgcaga
    70861 aattatattg cttagaattg aatcaggttg tgtaatcaca aacaccgtat ctgttgaaca
    70921 gttgtaactg tccgtaacga ttactttgta attgcctgct gtaagattgc tgattgatgg
    70981 tgttgtcata ccgtttgtcc agatgtagga gtaaggagca ataccacctg atgcagttac
    71041 tgaagcagag cctgtgttat tgccatgaca gttaatattt gtttgttgtc cggttaatga
    71101 taaagcttgt gtaggaccat taatagtaaa cgtttgtgag aaagtacaac ctgcactatc
    71161 agttattgta accgtatatt gacctgcacc aatgtttacc agattttctg tcgtgtcgcc
    71221 attgctccag ctataagagt atggcactgt tccacctgta ggattcaaca ttattgatcc
    71281 tgatgtaaga gatgaacaat caagatttgt tacaacaggg aaggcatata atgcaccttc
    71341 cggtgtagga acgtaagttg aagtctgcgc aatacaactg ttagagtcaa caatatatac
    71401 agagtatgaa ccacctgtta agttagtcag gttggcagta gtgtcaccat tgctccaata
    71461 gtattgataa ggtggtgttc caccggtaac agtaagatta attgcacctg tgctgtcagc
    71521 aaaacatcct accggagtca taacaatact tggagaaagc ggaccattag gttgtgtcac
    71581 tgctgtcaca aggctgaatg tacatccgtt tgcatcagtt attgaaacag tatagttccc
    71641 tgctgttaag ttcgataagt cttctgtaac atcacctgta ttccaaacat aactgtaagg
    71701 catggtacca cctgttacag taacatcaac agcgccattg ttaccaccga aacaacttac
    71761 tgatgatgtc atctgatagg ttgcattcac tgctgctgcc ggttgttgaa cttcaataga
    71821 gtcagttgca ctacatccat ttgcatcagt cacagtaaca gaataccatc ctgaagcaag
    71881 gttgctgata ttttgagtgt tgtttccatt actccagaag taagtgtaag gacttgttcc
    71941 accaacagga tttgtattaa tcgctccatt gtttccacca aaacagttta cattttgttg
    72001 tagtgaaaga ttgttactta atgccaatgc aggttgtgaa agatttgcag aaattgacca
    72061 tgtacaaccg ttctgatcag taacagttac ctcatataaa ccggaagtaa tgttgttaag
    72121 atcctgtgtt gtctgaccgg aattccacag ataagtataa ggagttgtac caccgttaac
    72181 cgtaagatca atgtttcctg atgcattacc aaagcagcct atattaccta ttgatgagat
    72241 tccaccggat atgctgtctt gtggctgatt aattgtcaca gactgattag atgtacaacc
    72301 attagcatca gtaatagtta cattataact tcctgcagct aatgatggtt gattttgtgt
    72361 tgttgaacca ttgctccaca caattgagta aggagttgtt ccaccttgta tgtcaagatt
    72421 tgcacttcct gtgttgccac cgaaacaaag cactggactt gttgaagtga tagaagaagt
    72481 aagaccagca gcaggttgtg ttaaagttat gttttgttga actgtacagt tgtttgcatc
    72541 tgtaacagtt acactgtatg ttcctgcagc aatgttcgat aaatcttcag tgtttgcacc
    72601 gttgctccag ttaaaattat aaggagttgt accgcctgtt attgtaagat caatgtttcc
    72661 tgttgtattt ccgaaacaaa gtatgttttg cattcctgta atacttgaag ctaaagctcc
    72721 tgcaggttgt gaaatggtta cagtttgatt gttgtaacat ccgtttgcat ctgttacagt
    72781 tactgtataa attcctgagg caagatttga aatgtcttct gttgttgctc cattgctcca
    72841 tacaaatgta tagggtgccg ttcctcctga tgcattcaga tcaatagctc ctgtagcatt
    72901 gctaaagcaa tcaacatttt gtgatgattg aattgaagtt gcaaggtttg acggaggctg
    72961 tcctacattt tctgtcagag tatatgtaca gccattagcg tcagtaatcg ttacagtata
    73021 agctcctgtt ccaagatttg aaatatcttc acttactgat ccattactcc agttgaatga
    73081 atatggcatt gttccaccat tgactgtgat atcaattgag ccgtttccac cacctgtaca
    73141 cgatacattc tgatttgatg taactgttgc tcctaatcct gcagccggct gactgattgc
    73201 aattcctgca atggaagtag tacaaccgtt ggcatctgta atatttactg tgtaatttcc
    73261 ggcaccaaca ccaatcaggt tctgacttgt gcttccatca ctccacaaat aggagtaagg
    73321 cgtagtacca ccatttacat taattaaaat cgctgctgtg ctttgaccat agcaaccaac
    73381 attggttaag ttgttgatgc ttgatgtcag tgttgcagat ggttgagtta aagtagccga
    73441 ttgtgatgat gtacaaccat tggcatctgt gattgttact gtgtaagttc ctgcggcaat
    73501 ggcattcaga tcttctgatg ttgcaccatt gctccagttg taattgtatg gtgtagttcc
    73561 tccatttgct gacaagtcta ttgatcctgt tgcattacca aagcagttaa tattttgtga
    73621 tgatgttatt gctgaagata aagaagtgga aggctgtgtg ataatttctg aaagtgaaaa
    73681 cgtacatccg ttggcatcag taattgttac agcgtagctg cctgatgtaa gtccagatat
    73741 gttttgagtt gtagcaccat tgctccagtt atagctgaat ggtgatgttc caccacttac
    73801 attaagacta attgatccgt tattgccacc tgtacaatta acattcactg cactgtttac
    73861 actgccaaat aatactgcag caggttcgct gatgatgatg ttgttaattg atgaagtaca
    73921 gttgttggca tctgtaacag ttacactgta tgttcctgca gaaaggctgc taaggttttg
    73981 agtgattgcg ccattgctcc aattgtagga gtaaggagct gttccaccgt ttacattgag
    74041 tgtgatgttt cctgtgcttt gtccgtaaca gttaacattc tgagttcctg aaattgaagt
    74101 agctaatgca cctgtaggtt gtgttaatgc aattcctgaa agtaaattgc tacatccgtt
    74161 agcatcagta attgttactg aatatgttcc tgcaccaatg tttgaaatat tttgtgtagt
    74221 tgcaccattg ctccagttgt aggtgtaagg tgctgttccg ccatttacag acaatgtaat
    74281 agagcctgtt tgatttccga aacaattgat attttgcgaa gaagaaactg ctgaatttaa
    74341 tgccccggca ggctggctta ctgttatact tgcggaagta ttcgtacatc cattgccatc
    74401 tgtaatagta actacataag tgcctgatgc aattcctgaa atatcttctg tagtagcacc
    74461 attgctccaa ctgaaagaat agggagctgt tccacctgta actgttaagt caatgccacc
    74521 tgtattattg ccaaaacaac ctgcatcact tgttgtaaat gtataagcta atgcagcagc
    74581 aggttgcgtt aatgaaacac ttcctgttga tgatgtacat ccattggcat ctgtaattgt
    74641 taatgtataa gttcctgcag caaggttgct gagattctga gatgttgcac cattgctcca
    74701 gttgtagctg tatggtgttg taccacctgt aacatttaca gaaatacttc ctgtgtttac
    74761 attataacaa tttatatttt gtgccgtgaa agtattgttc agagcagctg ttggctgtgt
    74821 aatttgtagc gaagttgaag cggatgtaca tccgttggca tctgtaacag ttacagaata
    74881 tgttcctgca ccaatgtttg aaatattttg tgtagttgca ccattactcc aattgtatga
    74941 ataaggcgca gttccaccgg taacatttag tgtgatagca ccggtatagt tatcgtaaca
    75001 gtttaccgca gttaactgtg gtgttacagc taatgcaccg cttggttgtg taagtacaat
    75061 gccattcatc tgtgttgtac aaccgttggc atctgtaata cttacactat aagtgccggc
    75121 tgtaatattt gtaatatctt gtgatgttgc accattgctc cagttgtagg tgaaaggtgc
    75181 tgttcctccg gttacgctca catcaatact tcctgtactt tgattgtagc aaagaatatt
    75241 ttgtgatgct gatatgtttg cagccaatgc acctgatggc tgtgcaatta caaccgatga
    75301 ctgactgata caattatttg catcggtaac agtaactgca taattacctg ccgacaggtt
    75361 tgataagttt tgagttgttg cactattgct ccaactataa gtgtatggag ctgttccacc
    75421 aacaactgat aatgttactg aaccattgtt gccaccataa caaagaatat cctgagaaga
    75481 agctactgat gatgtcaatg cacctgaggg ttgattaact acacccgtag cagccgcagt
    75541 acatccattt gcatctgtaa tagatacatt ataagttcct gcatacagtc ctgaaatatt
    75601 ttgtgttgtt gcaccattac tccagttgta tgtgtatggt aaagtaccac cggcaacatt
    75661 aagtgttatc gaaccatttc cgccaccatt acaagcaacg ttctgtgatg aagcaacact
    75721 tgtagttaat gggttgtcag gttgattaac caacacaccg gttatggcct tgctgcagtt
    75781 gtttgcgtca gtaatagtaa ctgaataggt tcctgcagta atacttgtaa tattttgagt
    75841 tgtagcacca ttgctccata agtaagtgta aggtgctgtt ccaccgctta cagcaagtgt
    75901 cagacttccg ttgttacctc cataacatag aacagaaacg tttgtagcaa cattggcatc
    75961 taaaattgcg gcaggttgat ttacctgtat tgcagcctgg cttgtagtac aaccttgcgc
    76021 atcagtaact gttacactat aagttccaga atttacattc gataaacctg aagttgttgc
    76081 acctgtattc cataagtatg aatatggagc agtaccacct gatactgttg ttgaaattgc
    76141 tcctgtagat aaaccaaagc ataatacatc tgtagtgttt atagatgtgg ccaaggctgc
    76201 tgccggttgc gaaattgtta ttgcatttac ttgtgttgta caaccattag catcagtaat
    76261 tgttacagaa tagcttcctg ctgtcagatc attattattt tgtgttgttg caccattgct
    76321 ccatgcgtag gagtaaggtg atgttccacc gctaactgtt aagttgatgt ttccattatt
    76381 ttgtccataa caagaaacat tctgacttgt tgcaacactg gctgccaatg ctgccgttgg
    76441 ttgtgtgata gctttagtta atgttgttgt acaattgttg atatctgtta cagtaacagt
    76501 atatgtgcct gaagttatgc ctgaaatatt ttgtgttgtt gcaccattgc tccagttgta
    76561 tgaatatgga gtagtaccac cggaaacgct taattgaata gcagcattgt ttccaccaaa
    76621 gcaatttaca ttttgtgatg atgaaacaga tgcatttaat gcctgtgccg gttgtgaaac
    76681 tgttgcggtt tcagttgttg tacatccatt gttgtcggtt acagttacag tatatacacc
    76741 tgaacttaaa cctgtaatat tttgtgttgt tgcaccattg ctccatgcat aagtgtaagg
    76801 atttgttcca ccatttacat ttattgaaac agaaccattt gataatccaa agcaagaaac
    76861 atttgcagat gcagatatag ttgcatccaa ttgagctgat ggttgagtaa ttgttttgtc
    76921 agcagtagtt gtacatccgt tagcatctgt gatggtaaca gtgtagctgc ctgcagcaag
    76981 tcctgaaata gatgcagttg tagcaccgtt gttccataag tacgtgtaag ccgttgttcc
    77041 acctgatgca gtaacactca tgctaccgtt cggaacatta taacatgaaa tattttgtga
    77101 tgcagttacc gatgcactca atgcctgagc aggctgagta attgtttgaa ttgcatttgt
    77161 tgtacatcca ttggcatctg taacggtaac agaatatgat cctgctgcaa gaccacttat
    77221 gttttgtgta gttgcaccgt tgctccaact gtaggaataa ggagtagtac ctccaccaac
    77281 tgtgatggat attgatgcat tgctgccacc gaaacagttg acattctgtg ttgctgctat
    77341 gcttgcattc aatgcctgtg ccggctgacc aatagtttta gtcatacttg ttgtacaact
    77401 attcgcatcg gtaacagtta cagtataact gcctgcagaa agattttgta tattttgtgt
    77461 tgtagcaccg ttgctccagt tataagaata aggtgaggtg cctccgttaa cgtttatatt
    77521 tatttgtccg ttagttccac cattacaact tacttcttct gatgattcta aagttgcagc
    77581 taatgcagct gatggttgtg ttactgtttt gtttaatgaa gtagtacaac cattagcatc
    77641 agttacagta acagtataag tgcctgcaat aagacctgtt aaattttgtg ttgttgctcc
    77701 tgtactccac gagtatgtaa atggcgaagt accacctgca acacctacat taatgctgcc
    77761 attacttaaa ccaaaacatg aaacttctgc tgatgcagat accgaagcag ctaatgcacc
    77821 tgaaggttgt gaaatattct tgttaattgt gtagatacat ccattatcat cggtaacagt
    77881 aacagtataa ttacctgcag taatgttgct gatgttttgt gttgttgcac cattactcca
    77941 gttaaatgta taaggtgaag tgccaccgga tacagatagg ttgatgtgtg cattgtttcc
    78001 tccaaaacac aatacatttt gtgatgatga gatgcttgcc gataatgcat ttgaaggttg
    78061 tgaaacagtt cctgttgctt gcaattcaca tcctttggaa tctgtgatgg ttacattata
    78121 aattcctgaa ctcaatccat taatattttg tgttgtagca ccgttgctcc ataagtacgt
    78181 gtaaggggct gttccgccag caacagtcat gttaagtgcg ccattgccac caccgttaca
    78241 agatacattt tgcattgatt gaatgtttgc actgatggct gcagaaggct gtgttaacgt
    78301 aatattattt gttgtgcttg tacaaccttt ggcatctgtt attgttactg aatatgtgcc
    78361 ggaagttaag ttggttaagt tttgcgttgt agcaccatta ctccagttaa atgaatatgg
    78421 agtagtacca cctgatacag aaatattgac agaacctgtt ccgtttccaa aacaattgat
    78481 gttttgtgtg gatgcaacag aactatttaa tgctgcagaa ggttgagtaa tgctgattgc
    78541 actttgtgat gaagtacatc catttgcatc tgtaacagtt acagaataat ttcctgcaac
    78601 tatgtttgaa atattttgag ttgttgcacc attactccat gtataagaat aaggtcctgt
    78661 gccaccattt actgtaagag aaacagcacc tgttgcattg ccaaagcaat taacattagt
    78721 gccagtggca gatgtggtta atgaggcaga aggttgtgtt acgcttattg aattttgtga
    78781 tgaagtacat ccattagcat cggtaacagt tacactgtaa acacctgcga tgatgttgct
    78841 taagttttgt gatgtagcac cggtattcca ggaataactg taaggtgatg ttccgccatt
    78901 aacagttaca tttattgaac ctgatgagtt tccgaaacaa tctactgcta caggacttgc
    78961 tgaagaggtt aatgaagcag aaggttgtgt aacagcaata gaattttgtg atgaagtaca
    79021 tccttttgaa tctgttactg taactgtata gtttcctgct gctatgttgc tgatgttttg
    79081 tgatgtagca ccattgctcc atgaatagga gtaaggcgca gtgccaccgg aaacactaag
    79141 attgattgca cctgtagcat ttccaaaaca gtttacattc actgctgttg gtgttgcagc
    79201 taaagctgca gccggttggc tgatgtttat cgaagtttgc gatgttgtac atgagttggc
    79261 atctgtaaca gtaactgtat agttgccagc tgttagtcct gtaacagatg aactggttgc
    79321 acccgtattc cataaataag tatagggtgt tgtaccacct gaagcagtta ctgatattga
    79381 gccgttattt cctccaaaac aatttacatt taccttcgag ttggttaatg agagtgctgc
    79441 agtaggttgt gtaattccaa ttgagctttg tgtgcttgta catccttttg aatctgtaac
    79501 tgtaacagtg taatttcctg ctgtcagtcc ggttacgttt tgtgttgttg caccattgct
    79561 ccatgtgtaa ttatatggag aagttccacc attcactgta agatttattg ctcctgttgc
    79621 accaccaaaa catccaaccc ctgttgcact tgctgatgca gataaagctg ctgcaggttg
    79681 tgtgagtgta gctgttgctg ttgtgctgca attttgcgag ctggtaacag ttactgtata
    79741 agtacccgca ccaacattgc tcaagttctg cgaagatgca ccattactcc atgagtaagt
    79801 gtatggtgca gaactgttgg aaacacttgt agttatacca cctgtctgct gaccaaaaca
    79861 attgatgttt gttcctgtca gacttacctg tggtgatttg cgaagattta taataatggt
    79921 gtcttttact gttgcgcaaa catttttagg tcccataatc aacattactg aacctgcatt
    79981 aatatctgct gctgatggaa tgtagtatcc gtttttaacg gtatcgcttg gactaaactg
    80041 acctgttcct gatgttgtcc attgaactgc accggtgaaa ctggattgcg aataggcaat
    80101 agttccgtta aatgctatag agtcctgacc acctcctttg gtacaaaggt ttttatctgt
    80161 tcctgcactg ttaactaaat agtccggtag gttgataata gtttttgaac ttaatttatg
    80221 tgcaaaccac catgtattaa tagtgttgct ttcacctata gtattttggt cagtgttggc
    80281 agggcttgat gtccacgtat gacaaggaga tggacaacca ccaagattag ttaagcctcc
    80341 aaccaaaaca tcatcataaa ataatgaatc tacaaactgt gcactaccgg gacgtataac
    80401 agacatcgaa aatcctgttt cgtgattttc tacatcatac attggcaggt tcaccaatcc
    80461 tgattcaata cttaatatta ctgatacagg atttcctaca gcaattaaat ttcctaaacc
    80521 atcttttcca tcccaataca aacaactgga accggcaggt acttgcggaa aaaataattt
    80581 ccggtctgca gtaccggcat caaatttatt attgttattt ctatcaatta caactgtaac
    80641 atcagatgtt tttatcaatc caacatttat acatattgaa tccctaacac aacgctccag
    80701 agtaggaggg cctgttaaaa acgacattga accgtttgga tatgcaataa tatccggatc
    80761 ggtaagaaac aaacgatatt ctacaggaac agaaaatccg gttttagatc ttctgtctgt
    80821 tgttgcattt ccggaattgg atgtaccaaa acgattacat gaaacacgaa atttaaaagg
    80881 cttaatacca ttcaggtcaa ctttagttac tgaactgtcc tggccataaa taaagaatga
    80941 tccataaaat ggatttgaat atgattctgt attgaaaccc cagttttttg aaaataaacg
    81001 acctgtaact ttagtgttac tagaagtatt aacaaccgtt acatcaaaaa tacctaagct
    81061 taattctgtg ttacctgcaa ctgttgttcc actaccttta ttaaattcaa tgtaataagc
    81121 accgggaaga cctatggtcg gattacaaac ataaccgtta taacccgctg catttaccac
    81181 attcggacct gcaatagctt gcgaatgata gctgataaat ccttgtgcat tacttgttgg
    81241 taatttttga ggaccaaaaa tgatattccc gtttggatct tttatacgca cataccaatc
    81301 ggaattagtt gaacgctgtc caattccaaa gtaaattttt tcttgtgcgg gattgttaat
    81361 ccgtacataa agcctgtcat cgtcagtggc cgaataaccc gcaaatgtac cataggtagt
    81421 ttgcgtattt ataatcataa ggttcgtggc ctttgttgta tcaggcttga actgtggtga
    81481 cccgtcagca tataatttat catggctgac aattagaaac aaagc
//
LOCUS       DAOOOI010000005        63316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_61_length_63316_cov_42.738654, whole genome shotgun sequence.
ACCESSION   DAOOOI010000005 DAOOOI010000000
VERSION     DAOOOI010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 63316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 63316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63316
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61_length_63316_cov_42.738654"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..768)
                     /locus_tag="PKX59_01765"
     CDS             complement(202..768)
                     /locus_tag="PKX59_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WcaF family extracellular polysaccharide
                     biosynthesis acetyltransferase"
                     /protein_id="HOZ89621.1"
                     /translation="MEERVKTDLSKFNPSEFDRGASSLKFFLWHWVNVIFFISPFCPS
                     SAIRCFLLRMFGARVGKGVVISKPGVNVKFPWRLTIGNHVWIGEKAWIYNLDQVTIGN
                     HVNIAQGAMILTGNHNYKSSKFETIFGPVTIEDGVFIGAKAIVCPNVTCYSHSILAAG
                     SVATSALKAYGIYQGNPAEYKKERVIQE"
     gene            complement(798..1358)
                     /locus_tag="PKX59_01770"
     CDS             complement(798..1358)
                     /locus_tag="PKX59_01770"
                     /inference="COORDINATES: protein motif:HMM:NF017816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HOZ89622.1"
                     /translation="MRFLTVFVICFVLSQTTNAQRELIEWNDSLSFKIEDYKGPITSP
                     DKSLHISTNCGLYCIPQIVGDTAILTIIAYMDKNKSWARKKDVTPALLEHEKGHFDLT
                     EVFARKMKKRVLALRTNKRTFIKDVQEVYDKTWEDLQKQHQAYDKATEFSNVEFAQRT
                     WQNYIKEHLVQYHFYASQTIRFPIKD"
     gene            1526..1822
                     /locus_tag="PKX59_01775"
     CDS             1526..1822
                     /locus_tag="PKX59_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOZ89623.1"
                     /translation="MFGKLAEAQKKMQEIKDRLETIIVTGESEQGKVIAEANGNNRIR
                     SITINESLFKVGDKNEVEELITVAVNRALEASEKLMQGEMAAASKDILPGFLQK"
     gene            complement(1832..2581)
                     /locus_tag="PKX59_01780"
     CDS             complement(1832..2581)
                     /locus_tag="PKX59_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89624.1"
                     /translation="MFAGSYYHCPMKYWSFKICAIAILCFFVDAIQAQTIHVVATVKG
                     NDATLPVVGLMIVNKSTGTGFFSPDNNFFSAVFNKSDTIMVNATGYSVKKICFKDSLL
                     RDTFRISIILEKPVVTLKPVTIIPPRELEVIQKDIEKLGYKKEDYMVTGIDAFESPIT
                     FLYQSFSRRERAKRDIAQKKNEDNKRKLLKELFRKYVDFDIMNLSEDQFDNFIDFINV
                     DDNFLKNSTQYDFILFVKKKFEMYKALNRAK"
     gene            complement(2657..9034)
                     /locus_tag="PKX59_01785"
     CDS             complement(2657..9034)
                     /locus_tag="PKX59_01785"
                     /inference="COORDINATES: protein motif:HMM:NF036499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOZ89625.1"
                     /translation="MAQKIYFANAVSEMTTEIRGVINRQFRKFKILSISLLFIHLLFA
                     ALSLLQVANAQTYAPVSYWTFNSSPFNKDSMNRTNMDFTSYGCGYTVSNNSAVGKGLI
                     LSSSGDNINTGNFVTDTSFAIEFLFKPGYQFNQTLLFRRMDGGFSANFNFDGIYFYTY
                     TSGSGSDELKVSFDGIGKRSYGYFVDGNWHHIVFQYSSLTGKKEIYIDGECPSGYSKN
                     ISGKVVNGANTSLFWNQGVSYMKYFGEYDEIAIYNRTVPAKLIYQHYKDAVQTNKSYS
                     FNLTFNGSLPTPSPVSAGLDNTEFAPGHPSPNVSAIDQLRSYPVARYKAGNTLMKNFN
                     WVDLMYMGGRFQSGVSDAQSATNSTAINEELAKNWNYMIQLRLSMDQWGNAWANLANQ
                     NPQLGTSLITLRAQLNPSLIRSQNLSSDKYLQNSSGNFIDPNGNNSSTKWWRPTAPVS
                     AYSGDGNQMKSNVQDVLNALTRPLTMINENGEVFAWSTDAALSADPAVVAAKNASGLD
                     YQTFQARKFKENEIQSYRDIIMDIPALSNTKFTEYGIDGFPQYRQKYSEARLVNSEIN
                     SQRYSTPDFYPRWPHNWRNWITAWHGWQWIVDSRYYELQAGDNLFSPFVAAGWDVNED
                     NNMRPAQFMGLLKCLNMIGAEFFYTGYFNESGNYNPPNPPPANPAGYAWQTAMPSYAQ
                     AVASRYEEFLRNGSVMLGDVPAYYSAPSGKPGYTFWAGDLRKLVVVRKHNTQSKFAIT
                     GTIQPNTSMVGGAENESVATISLDGQQVKFKVRRQGSTYIYDKTNSSSPVFYQLDEWH
                     EKSHPSFWSKDFTIESELYDNTNATYSLKTIQPQGAAAGDYSNFTTAVVFPSTQTTFT
                     PLEYVFQPRSSSQANLYLFVRARSTDGTATGLGVAVDNGASKSIGCVTDTNWTWYRLD
                     ACSQQAIQFTGLNVGSHTIRFTPTNNKLMIDKFILSANNNVITNYSMVACNSGGTATI
                     NAGGPTTFCQGGSVTLTAGAGNSYLWSNGATTQSITVSQAGNYVVTVSQGSGCAAISN
                     PVAVTVSSAPAATISANGSTSLCQGASVTLTASAGATYQWSNGATSQSITVNAAGNYV
                     VTVSNAQGCSATSAATSVTMTAGGTATITAGGPTTFCPGGSVTLTANTGTSYLWSTGA
                     TTKSITVNASGSYSVTVNNGSCSATSAPTSVTVSNSATATITANGPTTFCPGSNVILT
                     ANAGSSYQWSNGATSQSITVSAAGSYSVTVSNGNCSATSAPTVVTVSNNATATISAGG
                     PTTFCQGGSVVLTANTGSSYLWSNGATTKSITVNAAGSYSVTVATGSCSATSAPTVVI
                     VNQIPTATITAGGPTSFCQGGSVTLTSSTGNSYLWSNGATTKSITVSAAGSYSVTVSN
                     GNCSATSAPQNVAVASNPTATITPNGSTMLCSGASVVLKASSGSSYLWSTGATTQSIS
                     VSTAGSYTVKVYNAQGCVTTSAPVAVTTGSASATIVANGPTTFCQGGGVTLSAGAGTS
                     YLWSNGATTQDINVSTSGSYVVTVSNGSCSATSAPLVITVNPLPVATITASGSTTITQ
                     GQSVTLTASAGSSYLWSTGATTSSITVNTAGSYTVKVTNAQGCSATSSPMVITVVNNS
                     QPASITTTAANNQICMGQSATLTSSPGMQYIWLPGLQATQSIVVSAPGTYTVNIRNSD
                     GSTSTANITITQAPTPNKPEVTYTYVAGTMYQLKAFEPSAASYLWNGGQTTPTISVNA
                     AGVYSVVAKNAYGCQSPAAKVQVNSLEPASCAKPDMLSAYNIIDKKASLMWNPAVTAD
                     SFRVAYNVMGSSTVIVKYVRSRNNLDINELLPATTYQWKVTAICAGSSIVSATEVFTT
                     LNGPTDCGSTVVNTFTTNVTNSSAQLNWYGTRATSITIRYKKLGASSYTYKSLATNNT
                     TAWGFNLSGLVSGTTYQWSVKTECGSVSSSYSSDNYFTTTSGCDVISGIAVSNVMSNR
                     ATVSWNPQSGVSYVVVKYAPATSSQWKYAYVRASAGQTIIRNLTPGVQYNIQLKSMCT
                     YSNNSAYSAMERFTTGIARLGEATSELMINAYPNPADAQITYAFDAKKEGYFFIRVSD
                     IAGRILLNDKKMASEGTNGGDIDLRMFTPGVYTLTITKGSEENHIRFEVNR"
     gene            complement(9470..11053)
                     /locus_tag="PKX59_01790"
     CDS             complement(9470..11053)
                     /locus_tag="PKX59_01790"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89626.1"
                     /translation="MKCLTTIIVCIVFFIQATAQNNCSNPLEVTICPSVALFNQTNAG
                     MGDDLAPPCNIVGEDVLYKVNITNGASQIFVQVINCTGPFKVFATRTCGSCQSLGTNL
                     ITTLPNYYFLANASNDTVLYVWVDAATTVTYDISFGSDTNVAFVNIPSTQGNLSFDGS
                     VCATPTFEPTKPFFQLKVNGVYQTAPLFFSPLNVVDTVCITTFFKNTTGVEGVKKFVF
                     DFPASGYLNPQIAPIIPGFYNAGNWVATPSNGGKTWTLQFIDAAGIGKGDFTGTPNSC
                     LAYEFCFTLTPLSNNPLYTNTKVNITSDGFGKGYLGTMHQGCCTSFATICNMNFMGGG
                     AFGGTHSFGFGFNDPGALPVELLSFTAKAEKNRVKLNWSTASEKNNSHFTIEKSVNGN
                     NWSKLATVNGQGNSNSIQNYSYTDNYPVSGTSWYRLMQTDFDGTSETFPAQRVYVKPY
                     HYSIFPNPVKDLLTIQFDSDETPVIQIFNPVGNKVFAPFEWSNGMVKINTSQLPTGLY
                     FLSIETYGIAEKEQIAVVR"
     gene            11386..12102
                     /locus_tag="PKX59_01795"
     CDS             11386..12102
                     /locus_tag="PKX59_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOZ89627.1"
                     /translation="MNKEIIQGIRKELKNIANEEVRIIASRFFKPGEMAKVYGVKSPI
                     VRTIGKKYLQQVRTLPKKEIFVICEELWQSSYLEESVIACMFTESMHKKFEVGDIKVF
                     ESWINNYVNNWASCDTFCNHTVGDYLMMYPEKTELLKKWAVSSNRWLRRAAAVSLIIP
                     AKKGLFLPLIFDIANILLTDDDDLVQKGYGWMLKAACKHYEKDVFDFVMLHKSKMPRT
                     ALRYAIEKMPESLKFQAMKK"
     gene            12202..12900
                     /locus_tag="PKX59_01800"
     CDS             12202..12900
                     /locus_tag="PKX59_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOZ89628.1"
                     /translation="MKFTFQLFLLLLFTATHLRAQEENINTDRPDQSDGVYTLTKKHF
                     QIEEGVTIGKNLFVNNLMLRYGLTNSTEIRLLSDVGKIDESNGFLPLTISVKQKIIKQ
                     KNLIPAITAVGYMSYEKIASKDFQTNNISYELKLAFENEVNENFTVSYNAGLTDEFKT
                     FNYTLNLGYSPIEKLSLFAEYFGSVNSSVQHNADAGILILLYPKLQVDIACGHSIADS
                     DNRFYTTFGISYKF"
     gene            12908..13342
                     /locus_tag="PKX59_01805"
     CDS             12908..13342
                     /locus_tag="PKX59_01805"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HOZ89629.1"
                     /translation="MKYFTTHAFILLTFFTSNLFAQDNYIPVNQTPEQIKTYVKNHFP
                     ATIISSVKKEKELLNNELEVKLSDSTKLTFNKNNALIEIESNKPIPEKILADTIYDIA
                     HKNYPNNKIVGWEKTKLGQKVELDNDVELYFDDNGNFIRTEH"
     gene            13505..16354
                     /gene="carB"
                     /locus_tag="PKX59_01810"
     CDS             13505..16354
                     /gene="carB"
                     /locus_tag="PKX59_01810"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017260086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOZ89630.1"
                     /translation="MPRDNSIKSVLIIGSGPIIIGQACEFDYSGSQASRSLREEGIEV
                     TLINSNPATIMTDQVTADHIYLLPLTVDSIEKILNERKIDAVLPTMGGQTALNLCKEA
                     DERGIWTKYGVRIIGVNVAAIETTENRESFRKLMIEIGVGVPPSNVANSFLEGKEIAQ
                     RIGFPLVIRPSYTLGGTGGSFVMKKEEFEDRLMRGLQASPIHEVLVEKAVLGWKEYEL
                     ELLRDANDNVIIICSIENLDPMGVHTGDSITIAPAMTLSDHTYQRMRDMAIKMMRAIG
                     KFAGGCNVQFAVSPDTEDIIAVEINPRVSRSSALASKATGYPIAKIAAKLAIGYHLDE
                     LKNQITGSTSAFFEPALDYVIVKIPRWNFDKFHGADRTLGLQMKSVGEVMGIGRSFNE
                     ALQKACQSLEINRHGLGADGTGWHKVDDIIYSLEHPSWDRIFHVKDALKLGLPIQTIH
                     NHTKIDKWFLTEIQKLVGMEQELRRHSLKNLSPDLMLELKQNGFSDYQIAHILGNVTA
                     EEVYETRKEMGVKRIYKMVDTCSAEFAAKTPYFYSTFERPAQDSNGNEINASESIVSN
                     KKKILVLGSGPNRIGQGIEFDYSCVHGVLASKESGYEAIMVNCNPETVSTDFDIADKL
                     YFEPVYQEHIFELIEYEKPDGVIVQLGGQTALKLSEYLNEKGIKIIGTSYEDMDLAED
                     RGRFSDLLHDLGIPYPPYGVAESAEEALVVANKVGYPVLVRPSYVLGGQGMSIVINDE
                     ELERAVIKLLKNLPGNRVLIDHFLDRAEECEADLICDGKDVQIIGIMEHIEPAGIHSG
                     DSYAVLPPFSLSDVVISQIKDYAHKLALALNVKGLLNIQYAVKNEKVYVIEANPRASR
                     TVPFIAKAHQVPYINIATKVMLGVKKLKDFNIKPAEFGYAIKEPVFSFEKFADVNKEL
                     GPEMKSTGEAIRFIESTNDAYFRNLYKEKSMYLSK"
     gene            16372..16572
                     /locus_tag="PKX59_01815"
     CDS             16372..16572
                     /locus_tag="PKX59_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89631.1"
                     /translation="MRDVFYTIVVVWVVIQIYKSFIKPKSNTTQRNTTRQEGDVTINT
                     NTNNNNRNNDQGEYVDFEEIKD"
     gene            complement(16575..17735)
                     /locus_tag="PKX59_01820"
     CDS             complement(16575..17735)
                     /locus_tag="PKX59_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ89632.1"
                     /translation="MSGTVLAIAVTSIALSVMVMLVAVAIVTGFKKEITSKVVGFGAH
                     IRIKHFDNNQSYEEIPVNIDQKFINKLKSDNRISSVNAYATKAGIIRTSEDILGVAVK
                     GLDSTFNSGFFNPNMIEGSFNLQIHSGIYPAIISKSVADKMKLKLNDSFVVYFIDEPP
                     RARKFKVNGVYSTGLEEFDNLYVFCPLSVIQKLNNWNPLQAGGYEVNLKQLSDLESVN
                     ESVYKEAGFELNAQSIKTIYPEIFHWLDLQDVNVIIILVLMILVAGINMISTLLIILL
                     DNTTTIGLLKAIGAGNDGIRKIFLMVSSGIVGIGLIAGNVAGLLLCWLQYQFHLVSLP
                     QESYYVPYVPINLSVTNILLINAGTLVACILVMLIPSMIVSRISPVKALRYE"
     gene            17898..19016
                     /locus_tag="PKX59_01825"
     CDS             17898..19016
                     /locus_tag="PKX59_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOZ89633.1"
                     /translation="MLDSVITGAGQINNYLPIISDKNVAVVANQTSIVGQTHLVDLLL
                     SKHVKVKKIFTPEHGFRGVAEAGENIESGKDKSTGLPIISLYGNHKKPTTDDLKGIDI
                     VLFDLQDVGTRFYTYSSTLQYVMEACAENKKKLIVLDRPNPNGYYVDGPVLEPEFKSF
                     VGLNPIPVVHGLTMGEYANMLNGEGWLANKEQCDVTVISVKGYTHETRYKLTVNPSPN
                     LPNMTAVYLYPSLCFFEGTIVSIGRGTTYPFQVVGYPGFTEGPFEFTPKKNPEMKLPP
                     LYENQKCRGFDLRDFGNYYIPSLKKLYLFWIKNIYDNYQQKDKFFNDYFDKLAGTDKL
                     RKQIIAGLNEEQIRRTWEEGLKSYKVIRKKYLLYPDFE"
     gene            19118..20044
                     /locus_tag="PKX59_01830"
     CDS             19118..20044
                     /locus_tag="PKX59_01830"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOZ89634.1"
                     /translation="MKIYNHIDHFEKVSNAVITLGTFDGVHLGHKVIIERLKKIAVDV
                     NGQVIVLTFFPHPRMVLFPDDHGIQLLNTIDERTILMEKAGIDHLIIHPFSKEFSRMS
                     STEFVRDILVNKLGVKKLIIGYDHHFGRNREGSFTDLEELAPVYNFSVERIHEQLVND
                     VAVSSTKIRNALLAGDVATAREFLGYDYELTGTVVHGKKLGHSLGFPTANLHIREDYK
                     LVPANGIYAARTLVGGQWRDGVMSIGNRPTFDNGARSIEVNLFDFNSDIYGAKITIRM
                     SDFIRSEKKFENSAALIEAMNEDKLAAIKLLS"
     gene            20064..20666
                     /locus_tag="PKX59_01835"
     CDS             20064..20666
                     /locus_tag="PKX59_01835"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOZ89635.1"
                     /translation="MKRNIVIFLILLMTSTAHAQELWDDVAIDSLPVFHSLNAAMQNP
                     DSVFRLDLSKQKLKEIPAQVFELTQLRELNVSRNHLTELPEQVYKLNNLQRLYANNNK
                     LTALPQRIGTLKNLQTLTLDRNRIEALPPEIGMLHNLEVLSLWDNELSEIPDEIKSLS
                     SVLKKLELRGILFNEEQQKRIHDLLPYTTIYFSPACNCKQ"
     gene            20699..21217
                     /locus_tag="PKX59_01840"
     CDS             20699..21217
                     /locus_tag="PKX59_01840"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOZ89636.1"
                     /translation="MLQTDKLQLRACEPDDLPLMYQWENNSDYWLLSDTTKPFSNYTI
                     SEFIKQDQNDIYTTKQQRFVMELRNPVKPIGFIDLYQFEAVHRRAGVGILIGDLSEQK
                     KGYAAQSLECLKNYSHTVLNMHQLYCYIQSSNKKSLALFQQAGFKECGVLKDWLINKN
                     KPEDVVMFQFIF"
     gene            21302..21697
                     /locus_tag="PKX59_01845"
     CDS             21302..21697
                     /locus_tag="PKX59_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HOZ89637.1"
                     /translation="MQTLSETINKVKEFHATFGLQYFEKPQAAIDKNIIALRHKLMQE
                     ENDEYLEAAMNNDLEQIADALGDKLYILCGTIIAHGLQHKIADVFNEIHRSNMSKLDE
                     NGKPIYREDGKIMKGNNYFLPDIKAVLEQ"
     gene            21694..22404
                     /locus_tag="PKX59_01850"
     CDS             21694..22404
                     /locus_tag="PKX59_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ89638.1"
                     /translation="MIQKLSIIIPVFNESETIISVLEKVSDVQLINNIAKQIIIVDDH
                     SSDNSLEAITQFKNKHPQADITIVKQQVNMGKGYAVRTGMSKATGDYIIIQDADLELN
                     PDDFNILLKTSLDQNAQVVYGSRFLKKQQSQVPLLTLMANRILSMVTSLLTGKKVTDM
                     ETCYKLIDRSLLQNIQLKENRFGFEPEITIKLLKNNNIHFVEVPISYQPRTKKMGKKI
                     GVKDGIRALYCLTRYRFS"
     gene            22401..24173
                     /locus_tag="PKX59_01855"
     CDS             22401..24173
                     /locus_tag="PKX59_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89639.1"
                     /translation="MKRVYIFFALFAFLTLLAYLWQQRTFGNEKYERIVDSDGKGYVA
                     YLPALFIYQDLSFGFYSKPQNDKIARYFNDRFLTKQKDKTILKTYVGVAALMAPFYLT
                     GCLTQIMIHGITNGYEPMVQFFISMAAIFYALAGLFLIYKTLHLLNFSKTISALTSTL
                     IFAATNLCYYTLFHPTMSHVYSFFLISAFVYLSVCLTKNTNKKHLLLSAVTLGLIVIV
                     RPLNIIIILFPLLLLGKQKLTDLLKTKTGLLIVSALFFLMIISIQPILNYIQCGLLFP
                     WSYGDEGFYFTNPSLLKILFSFRNGLFIYSPLLFLSIPGLWLLRDSSIKITAATSLLL
                     IIVWYLLAAWWNWSSEPAFGNRSFVDWYGLMSLPIAASIGYIWNKSKVFLSILCFGFT
                     GLSTIQTWQYLNDIIHPDGMNFEKYRYVFLKTSPEYVASVGNGNEEAYLPEQRLPFFT
                     TKLNPENKSSNNHTETIFEFNAQKNKGAQFSITFSKNQLLPMKAMVTLQRKELEFKAC
                     TQATVCFDYADSGGSVYYNENLRINDIPRKGINEWRTFTYQFIMPALKDSNSQLNFYI
                     NNPKGARFEIKNIELKLFNFSNKQ"
     gene            24216..25652
                     /locus_tag="PKX59_01860"
     CDS             24216..25652
                     /locus_tag="PKX59_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOZ89640.1"
                     /translation="MQRKFITNLALLLLLNLLIKPFWIFGIDRTVQNMIGAEGYGKYY
                     ALLNFSFLLNILLDAGITNYNNRNISQHQHLVSKHFSGIIILRFLLAGAYIIISLILG
                     FAIGYSKEDIHLLILLLANQFFISFILYLRSNLSGLHLFKRDSIISVTDRFLMILICG
                     YLIWYNNGTYLNIENFVHAQSASYLITAVVAITFLHGRISFQKLNWQPAFYIAILKKS
                     LPFALLILLMTFYSRIDSIMLERLLPDGAFYAGVYAQAYRILDAVNMFGFLFASLLLP
                     MFSHMLKKRQDIQPLTNLAFKLLMIPALTLAALGLFFSNDIMNLLYHEHGDLSAPVFT
                     MLMFSFVCISTSYIFGTLLTANGSLRNLNLTALGGMILNISLNLFLIPQFKASGAALA
                     GLITQIIMALAQIILVAKILHWKPDWKLNGKLLLFFILIALVADLAVRYYPEIKTTIA
                     IIILAPILAFSLGLLRISELKSIMKERQ"
     gene            25703..26674
                     /locus_tag="PKX59_01865"
     CDS             25703..26674
                     /locus_tag="PKX59_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOZ89641.1"
                     /translation="MNNAPVYEENFASLSLIQLIKKWKKQLMIVAVISIVLSFIFTLP
                     FFIPPMFKSTAIVYPENLSPYSTESATEQLLQLLESDEIKMELIKDFKLYAHYEIDST
                     KKTALTTVFKQLAGNLSINKTDYESVKIEMMDTDPLQAKQMADSIIEKGNRLARKLYR
                     EKTMEAYVVAKTQFDMKENELDSADKVLTELRMKSGIIEFSQQTREISRAYYTSILNK
                     SNNVRLEEMFQKLQQYGSDYVTLNEKINYTRGVYNNYKQYVENAEKDLTKELSYSNVV
                     TPPMIPDKKAYPVRSLIMLMFTASVVFLSFVVIIIYENSQEKKPQTN"
     gene            26742..28127
                     /locus_tag="PKX59_01870"
     CDS             26742..28127
                     /locus_tag="PKX59_01870"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOZ89642.1"
                     /translation="MAVFSLVNEFYWLLILPAGILIGYSALYHLDKLFLFTAFCTPLA
                     INLSKTDLGIGVSLPTEPILFGMMIIYLLSKFIDGRYEKSFIKHPITVLILIHLAWML
                     ITSFTSSMFLVSIKHFVARLWFVVIGFMLATSIFKDEKKMRQFIWFYAVGMAITIIYT
                     ITRHSINGFTQQSAHWVMTPFYNDHTEYAAVLALMLPPIFGLMLLKHKSFMYRFFAFL
                     ISGLVIVAIVLSYTRATWLSLAVALVSMLAYVFRVKTWAVVAIIISMVVGFQIFQSTV
                     LYKFGKTKEYSQNDYRGHLKSITNIKTDASNVERLNRWSCAWRMFLDKPVFGWGPGTY
                     QFQYSVYQLQFEKTYISTNLGDKGNAHSEYLGPLSEQGFMGTIIFVALAVVVLYKASR
                     VFLQTNSNNIKVLALSMQVGLITYLVHGFLNNFLDKDKASIPFWAFVAAITVMDYYHN
                     KKQLNPSTERA"
     gene            complement(28110..29336)
                     /locus_tag="PKX59_01875"
     CDS             complement(28110..29336)
                     /locus_tag="PKX59_01875"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOZ89643.1"
                     /translation="MEETTDKLAADLQSKKVQLHWLLQITKAINYNLPAEQLYQIYEN
                     VLHDHLKAGKLSLFVNENGWKQVLSYGMKEELGRLQPELFLNEISFSKSKTVATPQWL
                     EKDESVIPVYHNEMLLAYCIVGAIDPSQSISIKEVVPFIHTITNIIVVAIENKRLNKE
                     AVAQAGFRKELELAAEMQEMLFPVGLCGNEYFEIATHYLPHQQVGGDYYDFISLNKHE
                     HIFCMADVSGKGMAAALLMSNFQANLHALIKNRPVLTNLVAELNDCVSKTAKGEKFIT
                     FFIGLINFKKGEINYVNAGHNPPVFCSKNKSELLERGTTGLGMFDELPFINEGHVKIN
                     SGDSVFCYTDGITELENLKGDFYGMEALKLFIEQNMNNSSLDEFHKKLIADLDHFREG
                     NSFSDDVTILSLRSLS"
     gene            complement(29339..30277)
                     /locus_tag="PKX59_01880"
     CDS             complement(29339..30277)
                     /locus_tag="PKX59_01880"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOZ89644.1"
                     /translation="MKILPAIRLADVASLLNAKYIGDAGFQITGINEIHKVKKGDITF
                     VDHPKYYDKALNSAATTILINKEVSCPEGKALIISDDPFRDYNLLTKKYRPLTFSMQQ
                     VDQTAVIGEGTVLMQGSVVGAHSKVGKNCLIYPGVVIYDHCEIGDGVIIHANSVIGAD
                     AFYFKRRPEGFDKMHTCGRVIIGDYVEIGACCTVDKGVSGDTVIGSGTRMDNHVHVGH
                     DTVIGKNCLFAAQVGIAGVVTIEDDVILWGQVGVQKDLTIGKGAVVLGQSGVGKTLDA
                     GKTYFGSPTTDARLKMRELALMKRLPEVFEFLDNKE"
     gene            30475..32835
                     /locus_tag="PKX59_01885"
     CDS             30475..32835
                     /locus_tag="PKX59_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ89645.1"
                     /translation="MKRFIALTYALLCFTLAFAQKPTQTVRGTITDQQSKEPLIGASI
                     VWLDSSTFIGATADINGNFRLNNIPVGRQAFKISFLGYRENVITSIITSGKEVVLNIE
                     LEQSVVTAKAVTIVAEKQKDKPNNELTTVSARSFTIEETSRYAGSVGDPSRMAANYAG
                     VSGANDSRNDIIIRGNSPTGLLWRLNGVEIPNPNHFGSLGTTGGPISILNNNVLDKSD
                     FLTGAFPAEYGNVLAGVFDLQMRTGNNEKHEFLAQVGFNGFEAGAEGPISKKQGSSYL
                     INYRYSTLGVFNALGINFGTGTAIPKYQDVSFNIKIPTNKAGKFTLFGMGGISGIEVL
                     EKDRDTTQKNLYVDDKRQNGYFDNTMGVVGLMHTFFINNSTYTKFSLSASIAGQKYKV
                     DTLPDFVDYAVPYYRNKSDQQKYSADFSLNKKFSSKDFLKTGFSFSQYHYTYRDSGVV
                     DSVWESYTDYNGNALLTKLYVEWQHRFSDQLSLNSGVYTQLFNYNNTYSIEPRIGAKW
                     EISDKQSLSLGTGLHSQLQPMYIYFVKTMKDDGSYYETNKNLKMTKSYHAVLAYDRNI
                     SKVFRIKTEVYYQYLFDAPVEKRPTFFSVLNEGADFGIAGIDSLENKGTGYNYGFEFT
                     AEKFYSKGYYFLFTASLFESKYKGSDGVERSTAFNGNFVFNLLGGKEWTIRKKNTISI
                     NIKTTYAGGRRYIPIDFAASQITGETEYDEDHAFEHRRKDYFRTDLKIGYRINRKRLT
                     HEIAIDLNNVFNTQNIWNQQYSAKTNTVKTEYQMGFLPIPMYKCTF"
     gene            32946..34739
                     /gene="mutL"
                     /locus_tag="PKX59_01890"
     CDS             32946..34739
                     /gene="mutL"
                     /locus_tag="PKX59_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOZ89646.1"
                     /translation="MADVIQLLSDTVANQIAAGEVILRPSSVIKELMENAVDAGATRI
                     DVSINDGGKNLIRITDNGCGMSETDARLCFERYATSKLRKADDLYAIRTMGFRGEAMA
                     SVAAVCQVELKTKREEDETGTRIEIEGSEFKIQEPCVMQSGTIITVKNIFFNVPARRN
                     FLKATPVEARHVMDEFLRVALPRPEIGFSLQHNGMEVYNLPATHQRQRICQVFGATYN
                     ERLVPVEEDTTILNIKGFVGKPEFAKKVRGEQYFFINKRFVKDAYLNHAVTIAFNELL
                     PQGTFASYFLFFEVDPSRIDVNIHPTKTEVKLEDEKSVYAILRSSVKRALGRFSIAPT
                     LDFNQEQAFNVPLSQLNTIPVQPQVKVNTNYNPFKSTEINKPTQQQNGQWEKLYEIAS
                     TPDVQQTKLPIAPIEENNASAFMQLFNSYVAVAMPDEMWLIDQQLAHEKIVFEKTLLQ
                     MKGTPTVMQQSLFPQQIELDNRQAATVSSLLEDLNRSGFDLQPFGQNTFVLNAVPSYL
                     ETGTEKKIIEMLIDEFISGEASAYNPMERVAIAIARSACLKSGKVLSAREMSALFNDL
                     MQCEMPYFTKSGKAIIFKLEKNEIDRKFEKG"
     gene            34846..35685
                     /locus_tag="PKX59_01895"
     CDS             34846..35685
                     /locus_tag="PKX59_01895"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOZ89647.1"
                     /translation="MQEFRPSSFKLLPDVIKNIMIVSALAFFADLALNKIGFDLNAYA
                     GLYLPSSEHFHWWQYFSYMFLHGSFSHILLNMFAFWMFGGILENLWGPKRFLIFFLVT
                     GLGAAFIHTAVGYFELHSLQAKADAFFNAPGADAFSVFVRDHIPYQYLNADYAMAVEN
                     IKSNWYAHGDSPMIIEQARQAIDEYIRFKTNIPTVGASGAVYGVLMAFGILFPNTLIY
                     LYFFIPLKAKYLVLIIGGIELYSAWQSNPTDNVAHFAHLGGMLFGYLLLRYWQKNNRS
                     QLF"
     gene            35700..36572
                     /locus_tag="PKX59_01900"
     CDS             35700..36572
                     /locus_tag="PKX59_01900"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOZ89648.1"
                     /translation="MKSNYKSLDMLQKIIVVNAIIYLFVNIGDVVLRLFNFPDGTVSG
                     FLTDWLAVPSSLKSLLYRPWTLITYCFYHENFLHILFNMLWLFFMGRLFTEYLGEKKL
                     LPLYLVGGLCGSVLFIVFYNLFPLFSREVDIAVALGASASVLAVTVAIATLLPDYEVG
                     LFLPQWRVKLKYIAIALFVIDLLSITGNNAGGNIAHIGGAIFGFIYIKQLKKGRDLTG
                     WLQRMIDRITQKKKPSIKVVYRKDSSDESYHNKKANNQEAIDKILDKISRSGYASLTN
                     EEKEILFKASKNQD"
     gene            36572..37663
                     /locus_tag="PKX59_01905"
     CDS             36572..37663
                     /locus_tag="PKX59_01905"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOZ89649.1"
                     /translation="MLKSALNLLLWLITIFIAVVTFMAVAAAYISPAKIWFLALFGLI
                     FIYIYLVAFIWMLVLSFRHKKMMLLILIPLIAGLPTFVAHWNYSSSNQKATGENTIKV
                     LSFNVRVFDLYNWTHNLQTRSNIFNFLKKSDAGIYCFQEYYTSKLMPFNNTDSLKSLL
                     GISNVHTMLPIIERDSDEWGIATFSKYPILNKETVFTDKESANGCIATDLLIGNDTVR
                     VYNIHLQSVRLAKNDYIFVSEFNNKDNTQKMKGSKQIVQRLKKAFIKRAHQVCKVTTH
                     IGACPYKVIVAGDFNDTPASYAYNQISNGLTDSFLSCGKGLSSTYAGMFPGLRIDYIL
                     HSKSIKTIEYIRPLVHLSDHYPVISTLNF"
     gene            37775..38509
                     /locus_tag="PKX59_01910"
     CDS             37775..38509
                     /locus_tag="PKX59_01910"
                     /inference="COORDINATES: protein motif:HMM:NF013997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOZ89650.1"
                     /translation="MRVKISFTRDNTSGNSIPLHHQKLIYDSLKTVVHEVGGNMKQLN
                     FSSLKGTSKIQNGFMKFLSSKITLVISGSNDDLITKVSNRIFEKNFMPIGRMNLLPKG
                     KEITSDPEFTTQMRYLCISPLVLFDPIKDAARSTEQIDPTSHNFSDILFNTTLDAMEK
                     SGVPEATISSYAEFDVQPDAEYMQKAAAANKKFARVYRSAEGKNMIGYLVPFTLHAHP
                     DVHRYVWHNGIGVMTHEGYGMLDLAK"
     gene            38633..40507
                     /locus_tag="PKX59_01915"
     CDS             38633..40507
                     /locus_tag="PKX59_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOZ89651.1"
                     /translation="MLLRESIVRNKVNSFCTSFHNKTGGNINIGKIQFNGLRVIEIEN
                     ISLVSPLNDTLLHTDTFMVQLSLLKLIRLKNPVSNFSAHGIKIKIASGDSISNYSFLF
                     RKSKPENTEITSVQSTQASKVFTLWNKLTELSDLSFDLERCKITIIRNKETRNFYFPE
                     IINRRGKCRFIAYSNDHLQTEKWQAGFNIDDDKNEMSLNIKKLAGENWLPFFDVDNKP
                     KIAYSAISLQIKQPSRKDTTFQVKAELDSLSVNYWRIANDDVDFNKLSFSGICSFNNQ
                     RIETDSAVNITINKIHFKTKAAFDRKQPHSYALSLAFDESADDFFESLPKGIFYHFKN
                     FKAKGRLKFDLNFALDMSAPDSLIFNSDLTGNALTIEKYGDENFSRINNTFLFDAYDG
                     DRLQRSFLIGPENSDFTPLNEISHYLQYAVLTSEDPSFFYHRGFIPDAFKESMIENIK
                     AKRFVRGGSTISMQLVKNVFLSREKTISRKLQEMLIVWLIENKGLVSKERMFEIYLNA
                     IEWGPGIYGIKEASQFYFNKLPSQLNLAESIFLASIIPRPKYYRYSFDAQGNLKSYLA
                     SYYRTVSNRMLSKTWISPIDTFNLQPQVKLKGPALKIVMPADSSMTDSLQWNQSNELF
                     "
     gene            complement(40513..41031)
                     /locus_tag="PKX59_01920"
     CDS             complement(40513..41031)
                     /locus_tag="PKX59_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOZ89652.1"
                     /translation="MKLLLIMFAFLFGPASTATSIYEFKVDGLSGGQIDFSKYKGKKI
                     LIVNTASKCGYTPQYSDLEKLYEKYKDKLVIVGFPANNFLWQEPGSNNEIAEFCKKNY
                     DVTFPMAAKVSVKGRDMHPLFRWLIEQPNVDFTGGIKWNFEKFLIDENGKLIHRFRSS
                     VLPTSPEITKYL"
     gene            complement(41077..42384)
                     /gene="rimO"
                     /locus_tag="PKX59_01925"
     CDS             complement(41077..42384)
                     /gene="rimO"
                     /locus_tag="PKX59_01925"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOZ89653.1"
                     /translation="MRTKTSKQLKVNVVTLGCSKNVVDSEVLMGQLRANEIEVEHESK
                     RQSEIVIINTCGFIDNAKQESIDTILHYAKEKEKGKIEKLIVTGCLSERYKPDLEKEI
                     KGIDNIFGTRDLPLLLKTLGADYKHELIGERLVTTPRHFAYMKISEGCDRPCSFCAIP
                     LMRGNHISKPIEELVHEARHLAAKGTKELLLIAQDSTFYGLDIYKERKLANLMEQLCS
                     VDGIEWIRLHYAFPSGFPLDVLDIMQRENKICKYLDIPLQHASDNMLKSMRRGITREK
                     TITLLDTVRNKIPDIAIRTTFISGYPGETEQDHETLIRFLEEMRFERVGVFTYSHEEN
                     THAYTLNDDVDEATKQRRLDELMQVQQNISDSINQKKVGNVFKTLFDRKEGNFFIGRT
                     EYDSPEVDNEVLVDANENYVRVGDFANIKITSAEAFDLYGTLC"
     gene            complement(42388..42891)
                     /locus_tag="PKX59_01930"
     CDS             complement(42388..42891)
                     /locus_tag="PKX59_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89654.1"
                     /translation="MHPHFKLLLAVFVLFSISSQAQRIDKSFRIKVSDNRNLQFTSYE
                     YLLKDDSMKISGVSDYGKSRVVYIAKKLKSKQKKAIRNFLKDFPVDSLQKEYFDDYAN
                     FGYIASDHFPRVIELEIRTGLKQHFSKASNAYVRSYARLIDFLNTLINDDVVKINYPE
                     SDFKKTF"
     gene            43107..43865
                     /locus_tag="PKX59_01935"
     CDS             43107..43865
                     /locus_tag="PKX59_01935"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOZ89655.1"
                     /translation="MSFRNILLAAAIVFTSCKQNAHLVKTETTTYPLNKPGFTEVDSS
                     ILNSYSSYKHTIDSIMDRVIIVSEMSMQKKTPEGLLGNFTSDAALKQVENDFHAKDFS
                     KIDFCFLNNGGLRAALPKGDITIGNIYSLMPFENEMVLLTLNGATTLKLINFIAAKGG
                     MPVSGLTMRVHESTVTEAKIQGMPFDSTKSYVVATSDYLANGGDNLSFLADAEKKVST
                     GLKLRDLLIQYLEEKNKRGEKLSAKIEGRIIYDK"
     gene            43855..44790
                     /locus_tag="PKX59_01940"
     CDS             43855..44790
                     /locus_tag="PKX59_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HOZ89656.1"
                     /translation="MTNRRDFLRTFAVGAAGLSLTGLPLEALAKRRVTKITILHTNDV
                     HSHIDPFPANDPKYPGLGGVAQRAAIINDIRNKERNVLLLDAGDIFQGTPYFNLFGGE
                     VELKLMSQLGYDAGTIGNHDFDNGVDGLSSQLKHANFPLIICNYDFKGTSMEGKTIPY
                     KIFEKEDVRIGVFGLGIELEGLVDKKMYGETKYLDPLEKAAETAHHLKFNEKCDLIIC
                     LSHLGYRYPNKKVSDDVLAKKSLNIDLIIGGHTHTFIDKPFVYKNRDNKDVLVAQVGW
                     AGIKLGRIDFFVDKKMRKLTADSTTLKVLNYARAI"
     gene            44953..46401
                     /gene="dnaA"
                     /locus_tag="PKX59_01945"
     CDS             44953..46401
                     /gene="dnaA"
                     /locus_tag="PKX59_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOZ89657.1"
                     /translation="MLKQTEKNVEKVWENCLKLIRDNVSPQNFKTWFEPIKPVKLEKH
                     VLTIEVPSQFVYEWLEEHYIQLLRKSIKKELGAEGRLEYSILMENNMNSSKPYAVKIP
                     AKGTGNLKNPQVAMPVSLNTAIKNPFIVPGLKKIDVDSQLNPNYSFDNFIEGDCNRLA
                     RSAGYAVANKPGGTSFNPLLLYGGTGLGKTHLAHAIGIEIKNNFQNKTVLYVPADRFM
                     NQFVDAVKNNSPNDFVHFYQMMDVLIIDDIHFLAGKERTQDVFFHIFNHLHQNNKQII
                     LTSDKAPVDLQGIEQRLLSRFKWGLSADLQSPDLETRIAILKRKMYNDGIELPTEIVE
                     YIAYSITTNIRELEGALISIIAQTSLNKKPITLELAKQMIDKFVKNTVHEISIDYIQK
                     IVCDYFELPLETLKSKTRKREVVQARQLAMYFSKSLTKSSLSAIGAHCGGKDHATVLH
                     ACRTVNNLMETDKKFKGYISDLQKRINLNHKN"
     gene            46397..46876
                     /locus_tag="PKX59_01950"
     CDS             46397..46876
                     /locus_tag="PKX59_01950"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HOZ89658.1"
                     /translation="MKVKILMVCLGNICRSPLAEGIMQHKLNQAGIAAQVDSAGLINY
                     HTGELPDKRSIAVAAKYGIDLTAQRARSFSKDDIKNFDYIFAMDNKNFENLMEYANNA
                     AEAKRIHLILDYAGLGKKIVPDPYYGDQSDFENVYSLLDDACQRILKKFAYAQSEVV"
     gene            46854..47588
                     /locus_tag="PKX59_01955"
     CDS             46854..47588
                     /locus_tag="PKX59_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOZ89659.1"
                     /translation="MLNPKLSDKKTKGILYLIPTAIGSMQAIDYLPQTVIEIIHSLDE
                     FIVENSKSARQFLKHCHIPTPQQQLTIHELNKHEGNVFEASFFSNLLSGKNVGLLSEA
                     GCPAVADPGALIVAEAHKLQIKVVPLTGPSSILLALMASGLNGQQFAFNGYLPKEKTE
                     RFAAIKKAEKDSAQRRQTQIFIETPYRNNALFDDLLNNLQPSTRLCIASALQCENEMI
                     KTQRISDWKKQRQELNKHPVVWLFLA"
     gene            complement(47748..47927)
                     /locus_tag="PKX59_01960"
     CDS             complement(47748..47927)
                     /locus_tag="PKX59_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89660.1"
                     /translation="MSILDGAMINSGKLSIKRPKYNFNISGKFRLTMSVNGMMPLQII
                     AANSMTTLSSWTEFL"
     gene            47920..48270
                     /locus_tag="PKX59_01965"
     CDS             47920..48270
                     /locus_tag="PKX59_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89661.1"
                     /translation="MLTILSMGYLNTENAHEMHLDKNYYLSKEQGLIKQKDNEATYKV
                     VKRMGIFNKTLVRNIFIGFVPDSAKILFLDEKSEIRLRAYMHGKTEIDSLDITTKTVA
                     SRDTILKIIKTQDK"
     gene            48267..49232
                     /locus_tag="PKX59_01970"
     CDS             48267..49232
                     /locus_tag="PKX59_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89662.1"
                     /translation="MTRFYLSVLAIFSVLTTSAQQMSTDIFLTNFGMNREGQYFFTAP
                     QNITHRPGYDNQPSFSKDGSKVYYVAYYDTLQSDIYVYSLEDSTNTRLTETPESEFSP
                     RVTADDLGFTIVRVDADKGQRFYKVLMDGTNEFQLLGVSDSVAYYCIANDSTYAVADL
                     NNNVMELNIYEMPSEQFIPLAKNIGRCIAVIPDGDNEISYVDKADTNGYMLMSFSINT
                     GLIGSVCQLKKGVEDYVWTKDGKLLIGYEGKLLMFDRNKPDNGWTELADFSKSVGNFY
                     RIAISQQGTQLALVAYTKEDKKQDDAKDDSGKKDDKKKRKKKKDE"
     gene            49322..50362
                     /locus_tag="PKX59_01975"
     CDS             49322..50362
                     /locus_tag="PKX59_01975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOZ89663.1"
                     /translation="MQTDYIIVGNGLAGSLLAWHLLLQEKNVVIIDEFNNTVASQVSA
                     GIFLPITGRRIVRTWMADELLAEVKTIFTQLGKLIGETYLYEMPVVEIPASVKEWNEW
                     QQRASSPDISKYISRFLAPNSIESVHCPFGAIELKNSGYVNISKCVTFLRKLYLDMEI
                     IIEGKFNFDDLQYSDKIVYKDIVADKIIFCEGAAGINNPFFNYLPFQLSKGEILTIRC
                     NTLSEEQIFNHGIFILPIGNQLFRVGSTYEWNDLTTNTTESARNNLSEKLQTVLKSDF
                     EIVDQKAGIRPSVKERRPFIGIHPKHKNIGIFNGLGTKGVMLAPYFAKHFAWHLAHQK
                     PLMADVDIARFS"
     gene            complement(50364..51539)
                     /locus_tag="PKX59_01980"
     CDS             complement(50364..51539)
                     /locus_tag="PKX59_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOZ89664.1"
                     /translation="MPSVSFNNLKVAFAYKSDADLKRAYWLFRLININFLVKIGPGFT
                     NAVLKMGLPIKPLIRATIYKHFCGGETIEQCTETINNLGKYRVGSILDYSVEGHHNES
                     DFDHSCAEIIKTVKHASGKKNIPFSVFKVTGVGRMDLLERVSSKVLLSEAEEAEFLRL
                     RARVESICKTAAENNVRLFIDAEETWIQDAIDNMVDDMMLKYNKEKTLIFNTVQLYRH
                     DRVAFMRENIAKARSGNYRVGYKLVRGAYMEKERERAAVNGYPSPIQPDKAACDHDYN
                     EAIKLCIENIDMVAVCAGTHNEDSSALLAKLMEEKGIANSDERVWFSQLLGMSDHISF
                     NLANAGYCVCKYVPYGPVVSVLPYLFRRAAENTSMSGQMGRELSMITQEIERRKKEK"
     gene            complement(51742..52539)
                     /gene="cdaA"
                     /locus_tag="PKX59_01985"
     CDS             complement(51742..52539)
                     /gene="cdaA"
                     /locus_tag="PKX59_01985"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOZ89665.1"
                     /translation="MELFHIGFLTVRLLDILDVLIVAWVLYKLYRLLKGGVAMNILIG
                     ILTIYFLWWLFVKVLDMQLLGALFGQFIGVGVIALIIVFQQELRRFLIFVGSNTIFTR
                     GDFFKNLLHGNFKLQQPVELSVMPIVRACVSMSKTKTGALIVVTRKTDLNLFEGTGDI
                     MEAEVSKRLLESIFFKNSPLHDGAVIISGNRIRAARCVLPVTESLDVPAHYGMRHRAA
                     LGITEQSDALALVVSEENGQISIAAGGQFYSNLSPDELEKKLKELME"
     gene            complement(52680..53915)
                     /locus_tag="PKX59_01990"
     CDS             complement(52680..53915)
                     /locus_tag="PKX59_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOZ89666.1"
                     /translation="MELKEILLWIIASILVLNYLLSMSLSILNLLNLSETQPDELKQY
                     YDEERYKKSQQYEREKSRFGMISETVMFVISFFFLIYGGYGWLDGWTHTVTTDLLWST
                     LLFFGTFALFSDLISIPFSLYSTFVIEERFGFNKTTLAVFISDRIKGYFLAGALGGSL
                     LAMFVLFYQYTGSNFWIYMLAVFAVFMIVLNMFYTSLILPLFNKLTPLADGEVRTAIE
                     NYCQKNKFEITNLYVMDGSRRSSKANAFFSGLGKKKKIVLFDTLVNNYSKDELVAVLA
                     HEVGHYKKKHTLFSMIFSILQTALLLWLLSVFINNEWFSLALGGTEYSLALSLIAFTL
                     LYSPLSLITGILMNIYSRKNEFEADNFARVTFSAEALASALKKLSSDSLSNLTPHPMY
                     VFFYYSHPPLLQRLKALQA"
     gene            complement(53906..55024)
                     /locus_tag="PKX59_01995"
     CDS             complement(53906..55024)
                     /locus_tag="PKX59_01995"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOZ89667.1"
                     /translation="MIHKAGISFLSTLFLFLLTVAAHGQQATHGAWMLGAEGHYGFVM
                     AHRPSVQPLQHSHLGGMMITLSQQADGKRSWHQHFHFPETGIKYGFFGLGNKEQLGYG
                     HTVYPYIDIPLGKSEKPFLHFQFGWGLGYITKPFNRNDNYQNVAIGSHLNAVIAFNFH
                     SEFWLTKRDRIVPAIGLTHFSNGSAAMPNLGINLITAQLHYSRSFGESKEIIRQETEN
                     FEKRNRFTIFGAWAFKQVYPLAGGTWYAWTLSPAFLRQYSPKSAAGISVDLFYDPTIN
                     YRLAYEDMKTHGTISNFRGGVAGAYEIIFSDFSVILQMGVYAKNAWKKDGLFYNRLGM
                     RYRFYDGLFACINLKTHIARADFIEWGIGYNFNKKAWN"
     gene            55016..55837
                     /gene="mazG"
                     /locus_tag="PKX59_02000"
     CDS             55016..55837
                     /gene="mazG"
                     /locus_tag="PKX59_02000"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOZ89668.1"
                     /translation="MNHTFAKKMLLAMEEKLNAFKRICTIMDELRTQCPWDKKQTIES
                     LRHLTIEEVYELSDAILDNNLEEVKKELGDIMLHLIFYSRIAEEKNLFNIADVINTEC
                     EKLISRHPHIYPAADGQKTVVADEEEVKRNWEKLKMKEGKISVLEGVPRSLPSMVKAQ
                     RIQEKARGAGFDWEKPEQVWEKVEEEFSEFQEAVSRKNQQEMTDELGDVFFALINYAR
                     FHNLNPDEALERTNRKFISRFRYMEDKVRESGKTLHELSLSQMDVYWNQAKTTEI"
     gene            55840..56526
                     /gene="trmD"
                     /locus_tag="PKX59_02005"
     CDS             55840..56526
                     /gene="trmD"
                     /locus_tag="PKX59_02005"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOZ89669.1"
                     /translation="MHIDIITVHPGLLEGPFSHSILKRAIQKNLVEINIINLRDYAAN
                     KHKSVDDYAFGGGAGMVMMIEPIDHCITDLKSKRNYDEVIYMSPDGELLNQGISNSLS
                     LKKNIIILCGHYKGVDERVRQHLITREISVGDYVLSGGELPAAVLCDSIIRIIPGVLN
                     DETSALSDSFQDNLLAPPVYTRPANYKGWDVPEVLLSGNESLINEWRHQQSLERTQIR
                     RPDLSDGNSK"
     gene            56653..57024
                     /gene="rplS"
                     /locus_tag="PKX59_02010"
     CDS             56653..57024
                     /gene="rplS"
                     /locus_tag="PKX59_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOZ89670.1"
                     /translation="MDLVKLAEKQLIPVKDAPDFKAGDTITVFYKIIEGNKEREQQFQ
                     GVVIQRKGHGATQTFTVRKISNGVGVERIFPLFSPKITKIDVNKRGVVRRARIYYLRN
                     VSGKAARIEEKIVKTAETKKK"
     gene            complement(57549..57932)
                     /locus_tag="PKX59_02015"
     CDS             complement(57549..57932)
                     /locus_tag="PKX59_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HOZ89671.1"
                     /translation="MDRRMFYGAKPEIFANAKWLRANMTQSEKLLWSHLKENKLGVRF
                     KPQHPIDIFIADFYCHELKLVIEIDGEIHEKQKEYDAGRTSELQHYDIEVMRFTNAEV
                     MQSIDKVLEQIKIRIDKRRLMDSAL"
     gene            complement(58088..58420)
                     /locus_tag="PKX59_02020"
     CDS             complement(58088..58420)
                     /locus_tag="PKX59_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOZ89672.1"
                     /translation="MYFENKELLRHILDEIEFVLFHTNGKSQNTVFNDAVLSRAIIRS
                     LEVIGEASKKIEPDFKTIHPHISWKEIAGTRDKLIHDYFGVDYDIVWDIIVNNLPDLK
                     IQIREVLE"
     gene            complement(58410..58718)
                     /locus_tag="PKX59_02025"
     CDS             complement(58410..58718)
                     /locus_tag="PKX59_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOZ89673.1"
                     /translation="MESDSINKNTIFLLLHKNKSQLLKYGVKQLGLFGSFVRNQQNEN
                     SDVDILVEFDEGKKNYKNFLELAYFLEGIFGRKVDLLTPQSLNKYIAPYILKETKYVF
                     "
     gene            complement(60216..61766)
                     /locus_tag="PKX59_02030"
     CDS             complement(60216..61766)
                     /locus_tag="PKX59_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89674.1"
                     /translation="MKKYILFILLIAVKISTAQQGINNNWLMGYSSFGGLPFGNTKFD
                     FFNGIPIINYDSLEMDFRHTHANISDAQGNLLFYTNGYYIADASNDTMQNGSGINPGA
                     YADQFSDGFQIPQGALIIRQPGSSSIYYMFHNTLDNHPVPGAYSLYFYLTTIDMSLNG
                     GLGTVISKNQILLTDSMVTGKITACKHANGRDWWVMTHQINTNKFFRFLVTTNNLILG
                     PYSQNIGAIRPQDAGQAWFSPDGSRFAYFYLNGGLDIFDFNRCTGQLSNPVHIAISFV
                     GGYNVGLAFSPNSDVLYVSNVFYVYQFDLTAANIAASVQTVATYDGFMSVPPNCPTCP
                     SLQTLFSNAGLAPDGKVYISTGNGTVHWHTIDNPDVVGMGCNVNQHSVQVPAYYFNTV
                     PNHPNYFLECDSTLGCLCAPTVGLPTPQPPKGGVAAMPNPSDGNFTLQFDVKSMSGEL
                     EIYDVMGKLVYKDYVSAWSQFKRVSLTPRDEVGGLTPLQGLGLSGGIYNCILKRGSEI
                     ERVKVIVN"
     gene            61969..62682
                     /locus_tag="PKX59_02035"
     CDS             61969..62682
                     /locus_tag="PKX59_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HOZ89675.1"
                     /translation="MIIEIWSDIMCPFCYIGKRHYENALKQFAGKNNIELKWKSYLLD
                     PETPLEFEKPLNVYEYLAERKGISLAQSIKMHEHVVAMAKQAGLDYQLDKLVIANMVK
                     AHRVIQFAKKKGLADLIEENMFKAYFTEGKNLADDDVLVAIGIASGLTKEEVITALTD
                     DLYAYEVDQDIQEARAIGLTGVPFFVFDRKYAISGAQPTEAFLNTLNKSYKEWLASNP
                     QPGLQITSGESCSTKGCDN"
     gene            complement(62700..>63316)
                     /locus_tag="PKX59_02040"
     CDS             complement(62700..>63316)
                     /locus_tag="PKX59_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ89676.1"
                     /translation="MYEKMKEGFEVVYAKRRSREGENFMKKFTARIFYRLLARITSVE
                     IPVDTGDFRIMDRKIVDVLKHMPEQQKYLRGQIPWIGFRQTYVEYDRKERLAGETGYT
                     YRKMMRFAIDGITSFSTAPLKFATASGFVVSFISFIWILYALYSRFIIKDYVPGWTSI
                     MIAVLFIGGVQLICIGIIGEYISRMSANIRNRPLYVVHDSNIQE"
BASE COUNT        20429 a        12016 c        11139 g        19732 t
ORIGIN      
        1 ggtaaagtga tagctctcat aaacatggtt ttttggttta taattatgtc ctgcatttat
       61 tcaaatgcaa accaaaatta agcccataaa atagaaattc caaatttatc tgagaaaaaa
      121 actcttttgt tatgataaaa ttttaaataa aatgatatgt tccatcactc aatcataata
      181 aaaacataat gttcctgttc tttactcctg tatcactctt tcctttttgt attcagcagg
      241 gttgccctga taaataccat aagctttaag tgctgatgta gctacagagc ctgcagccag
      301 tatgctatgc gaataacaag ttacgtttgg gcaaacaata gcctttgcgc ctataaaaac
      361 accatcttca atagtaacag gtccgaaaat ggtttcaaac ttcgaacttt tataattgtg
      421 attcccggtt agaatcatag caccctgtgc aatgttaaca tgattgccaa tagttacctg
      481 atcgagatta taaatccaag ctttttcgcc tatccacaca tgattgccaa tggtcagtcg
      541 ccaggggaat tttacattaa caccgggctt gctgatgact acacccttcc ctacccttgc
      601 accaaacatg cgcagcaaga aacagcgtat ggcacttgaa ggacaaaagg ggctgatgaa
      661 aaaaattaca ttgacccaat gccacagaaa aaattttaac gaagatgcgc cacggtcaaa
      721 ttctgatgga ttaaattttg acaaatcggt ctttacacgt tcttccattg gccaaaaata
      781 aaaaatgaaa caatgtgtta atcctttatc ggaaatcgta tggtctggct ggcataaaaa
      841 tgatattgca ccaaatgttc tttaatataa ttctgccagg ttcgctgtgc aaattctaca
      901 ttagaaaact ctgttgcctt atcgtaagcc tgatgttgct tttgcaagtc ttcccatgtt
      961 ttgtcataca cttcctgcac atctttaatg aaagtacgtt tattcgtcct taaagccaat
     1021 accctctttt tcattttacg ggcaaaaact tctgttaaat caaaatgacc tttttcatgt
     1081 tctaacaatg ctggcgttac atcttttttg cgtgcccacg atttgttttt atccatgtat
     1141 gcaataatgg ttagaatggc tgtgtcgcca acaatctgtg gaatacaata caagccgcag
     1201 ttggttgaaa tatgcaggct tttatctggc gatgttattg gacctttata atcttcaatt
     1261 ttaaatgata aagaatcgtt ccactctatc aattcgcgtt gggcattggt tgtctgactt
     1321 aaaacgaagc aaattacaaa tacagtcaaa aagcgcatac tttctaaaat tttagattct
     1381 tatacggccg ttctcctgtt tattttacat taatatctct ataaacctta catcaccctt
     1441 actgtttttt aaaatttgtc ggatgttttt ttggcgagaa ttcttgtgta tgactaactt
     1501 tgcagtcatt aaaaaccgaa taaatatgtt tggaaagtta gcagaggcac aaaagaaaat
     1561 gcaggagata aaagacagat tggaaacaat aattgtcacc ggagaatcgg aacaaggcaa
     1621 ggtgatagcc gaagccaatg gcaataaccg catccgttca ataactatca atgaatcgtt
     1681 gtttaaagta ggcgataaaa acgaagttga agagttgata accgttgctg taaaccgtgc
     1741 gcttgaagct tcagaaaaat tgatgcaagg cgaaatggct gcagcatcaa aagatattct
     1801 gccgggattt ttgcagaagt agttacagtt attatttcgc tctatttaaa gccttataca
     1861 tctcgaattt cttctttacg aaaagtataa aatcatactg cgtagaattt ttcagaaaat
     1921 tatcgtcaac attaatgaag tcaataaagt tgtcaaactg atcctcactt aggttcataa
     1981 tatcaaagtc aacatatttc ctgaacaatt ctttgagaag ttttctcttg ttgtcttcgt
     2041 ttttcttttg tgcaatgtct cgtttggctc tctctctcct gctgaaactc tgatacaaaa
     2101 aggtaatcgg gctttcaaat gcatcaattc ccgtaaccat gtaatcttct tttttatagc
     2161 ccaacttttc tatgtctttc tgaataactt caagttcgcg tggaggaatt atagttacag
     2221 gtttcagtgt aacaacaggt ttttcgagta taatggatat tctgaaagtg tctctgagta
     2281 atgaatcttt aaaacatatt ttcttaacgg aatagcctgt ggcatttacc attatagtgt
     2341 ccgacttatt aaatacagca gaaaaaaaat tgttatccgg agaaaaaaag ccagttccgg
     2401 ttgacttgtt tacaatcatc aatcctacta cgggtaatgt tgcatcattg cctttaacag
     2461 ttgcaactac atgaatggtt tgcgcctgta tggcatcaac aaaaaagcaa agaatggcaa
     2521 tggcacaaat cttaaaactc caatacttca tagggcaatg ataataactt cctgcaaaca
     2581 tctgcatgaa gattggtata aaaaaagagg aagccttttc aagcctcctc tcattttgaa
     2641 gttactgttt ctgtttttag cgattcactt caaatcttat atgattctct tcactacctt
     2701 tagtgatagt aagtgtataa acaccgggag taaacattct cagatcaatg tcgccaccat
     2761 tggttccttc agaagccatc ttcttatcgt tcaacaaaat tcgtcctgca atatcactca
     2821 ctctgataaa gaaataacct tctttctttg catcaaaggc ataagtaatt tgagcatcag
     2881 caggatttgg atatgcattt atcatcagtt cggaagtagc ttctcccaat cgtgcaatac
     2941 ctgttgtgaa acgctccatt gcgctataag ctgaattgtt cgaataggtg cacatagatt
     3001 ttagctgaat attatattgt actccgggag taagatttct gataattgtt tgtccggctg
     3061 atgctcttac ataggcatat ttccactgac tgcttgttgc aggtgcgtat tttactacaa
     3121 cataagaaac acctgattgt ggattccaac ttacagtagc tctgtttgac attacatttg
     3181 atacagcaat tccactaatc acatcgcatc cgcttgtagt agtaaaataa ttatcgctgc
     3241 tgtagctact tgaaacgcta ccacactcag tttttacact ccactgatat gttgttcctg
     3301 aaactaaacc tgataaatta aaaccccatg cagttgtgtt gttggttgca agtgatttat
     3361 acgtgtagct tgatgcacct aactttttgt aacgaatggt gatgcttgtt gcacgagttc
     3421 cataccagtt aagttgtgca gaactgtttg taacattggt agtgaaagta tttacaacgg
     3481 tactgccaca atctgtaggt ccattaagcg tagtaaacac ttctgttgca cttactatag
     3541 aacttcctgc acagattgca gttactttcc actgataggt agttgcgggt aacaattcgt
     3601 ttatatcaag attgttacgt gaacgtacat attttacaat cacagttgaa cttcccatta
     3661 cgttatatgc cactctgaat gaatcggcag ttacagcagg attccacatc aaagaagctt
     3721 ttttatcaat aatgttatag gcactcaaca tatcaggttt tgcacatgat gcaggctcta
     3781 aagaattaac ctgaacttta gctgcaggcg actggcatcc ataagcattc tttgcaacca
     3841 cagaataaac acctgctgca ttaacagaga ttgttggagt tgtctgacca ccattccaca
     3901 gatacgatgc tgcacttggt tcaaaagctt ttagctgata catagttcct gcaacataag
     3961 tgtaggtaac ttcaggctta tttggggttg gtgcctgagt gatggtgata ttagcagttg
     4021 aagtagagcc atcactgttt ctgatgttta cagtgtatgt acctggtgca ctaacaacaa
     4081 tactttgtgt agcttgtaat ccgggcaacc agatgtattg cataccggga ctagatgtaa
     4141 gtgttgcaga ttgtcccata caaatctgat tgttggcagc agtagtggta attgatgcag
     4201 gttgagaatt gtttacaaca gttatcacca ttggtgatga tgttgcagaa catccctgtg
     4261 cattagtaac cttaacagta taacttccgg cagtgtttac ggtaattgat gaagttgttg
     4321 caccggttga ccacagatag ctgctaccgg cagatgcagt taaagttaca gattgtcctt
     4381 gtgtgatggt agttgaacca cttgcagtta ttgtagcaac aggcaatgga ttaacagtaa
     4441 ttaccaacgg tgctgacgtt gccgaacaag aaccattaga aactgttaca acataacttc
     4501 ctgaagtgct cacattaata tcctgagtag ttgctccatt tgaccaaagg tatgaagttc
     4561 ctgcaccggc acttaaagta acaccaccac cctgacagaa agtagttggg ccattggcaa
     4621 caattgttgc agatgcactt cctgtagtaa cagccacagg tgctgatgta gttacacatc
     4681 cttgtgcatt ataaactttt acagtgtagc ttcctgcagt tgacacagag atagattgtg
     4741 ttgttgcacc ggttgaccat aagtaggatg aaccactgct tgctttcaat acaacacttg
     4801 ctcctgagca aagcatggta gaaccattag gtgtaattgt tgctgttgga tttgatgcta
     4861 cagcaacatt ttgtggagca gatgtagctg aacaatttcc attagataca gttacagaat
     4921 aagaacctgc tgcacttaca gtgatagatt ttgttgttgc tccgtttgac cacaaatatg
     4981 agtttccagt gctgctggtt aaagttacac taccaccctg acagaatgat gttggtcctc
     5041 ctgcagtaat tgttgcagtt ggaatttgat taactataac aactgttggt gctgaagttg
     5101 cagaacagct acctgtagca actgttactg aataagaacc tgcagcattt acagtgatag
     5161 attttgttgt tgcaccatta gaccaaaggt atgatgaacc tgtattggca gttaacacta
     5221 cacttccgcc ttgacagaaa gttgttgggc cacctgcaga aatagttgca gttgcattat
     5281 tgcttacagt aacaacagtt ggagcagatg tagcagaaca atttccatta gatacagtta
     5341 cagaatatga accggctgcg cttacagtta ttgattgcga agttgcaccg tttgaccatt
     5401 gatatgatga ccctgcatta gcagttaaaa taacattgct gcccggacag aaagttgtag
     5461 gtccgtttgc tgttattgtt gctgttgctg aattactaac tgttacagaa gttggagcag
     5521 atgttgcact gcacgatcca ttgttaactg taacagaata tgaacctgat gcattaacag
     5581 tgatagattt tgtagtagca cctgttgacc ataagtatga tgtacctgtg tttgccgtta
     5641 aagtcacact tccaccgggg cagaatgttg taggcccacc ggcagtaatg gtagcagttc
     5701 cacctgctgt catagtaaca cttgttgcag cagatgttgc tgagcaaccc tgtgcattgc
     5761 ttacagttac tacgtaattg ccagctgcgt taacagttat tgattgcgaa gtggcaccat
     5821 ttgaccattg ataagttgca cctgcacttg ctgttaatgt aacagaagca ccctgacaca
     5881 atgaagttga tccgtttgca gaaattgtag ctgcaggcgc agaacttaca gtaactgcta
     5941 ccggattgct gattgcagca catccacttc cttgtgaaac ggtaaccaca taatttcccg
     6001 cttgtgaaac agtgattgac tgtgtagttg caccattaga ccataagtag gaatttcctg
     6061 ctccggcagt taatgttacg cttccaccct gacagaatgt tgttggtcca cctgcattga
     6121 ttgtagctgt tccaccagaa ttacatgcaa ccattgaata gtttgtgatt acattattgt
     6181 ttgctgataa aatgaattta tcaatcatta acttattgtt tgtaggagta aatcgtatag
     6241 tatgacttcc aacatttaga cctgtaaact gaatagcctg ttgtgagcaa gcatctaaac
     6301 gataccatgt ccagttagtg tcagtaacac atccaataga ttttgatgca ccattgtcaa
     6361 cagcaacacc taaacctgtt gcagtaccat cagtacttct tgcacgaaca aacaagtata
     6421 agttagcctg cgatgagctt cttggttgaa acacatactc taatggagtg aaggtggttt
     6481 gtgttgaagg aaacacaact gcagtagtaa agtttgaata atctcctgca gcagcaccct
     6541 gaggttgaat agtttttaaa ctatatgttg cattggtatt atcatacaac tctgattcaa
     6601 tagtaaaatc ttttgaccag aatgatggat gcgatttttc atgccactca tctaactgat
     6661 aaaatacagg agatgatgaa tttgttttat cgtaaatata agtacttccc tgacgtctta
     6721 ctttgaattt tacttgctga ccatcaagag aaatagttgc aacactttca ttctctgcac
     6781 caccaaccat acttgtattt ggctgaatgg ttcctgtaat cgcaaattta gattgtgtat
     6841 tgtgttttct aactacaact aacttacgta agtcaccggc ccagaaagta tatccgggtt
     6901 ttcctgaagg agcactgtaa tatgcaggta catcaccaag cataacacta ccattgcgca
     6961 agaattcttc atagcggctt gcaacagctt gtgcataact tggcattgct gtttgccatg
     7021 catagcctgc tggatttgca ggtggcggat ttggaggatt gtaatttcct gattcgttaa
     7081 aataacctgt atagaaaaac tcagcaccaa tcatgttaag acatttcagc aatcccataa
     7141 actgtgctgg acgcatattg ttatcttcat taacatccca acctgctgca acaaacggag
     7201 agaataaatt atcaccggcc tgcaattcat aataacgact gtcaacaatc cactgccagc
     7261 catgccatgc agtaatccag ttacgccagt tgtgtggcca acgaggataa aaatccggtg
     7321 tgctataacg ctgactattg atttcagaat taactaaacg tgcttcgctg tatttctggc
     7381 gatattgagg aaaaccatca ataccatact ctgtaaattt tgtattcgac aatgcaggaa
     7441 tatccatgat tatatcacgg tatgattgta tttcattctc tttaaatttt cttgcctgaa
     7501 aagtctgata atccaaaccg gaagcattct ttgcagcaac aactgcaggg tcggctgata
     7561 atgcagcatc tgtactccat gcaaagactt caccattctc attaatcatg gttaatggtc
     7621 ttgtcaatgc attcaataca tcctgaacat ttgatttcat ttgattacca tcaccggaat
     7681 aagcacttac cggtgcagtt ggtctccacc atttggttga agagttgttt ccgtttggat
     7741 caataaagtt tcctgatgag ttttgaagat acttgtcact tgataaattc tgacttctga
     7801 ttaatgaagg attcaactgc gcacgaagtg taattaatga tgttcctaat tgtggattct
     7861 gatttgcaag gtttgcccaa gcatttcccc actgatccat gctaagacga agctgaatca
     7921 tgtagttcca gttttttgca agctcttcgt ttattgcagt actgtttgtt gcagactgtg
     7981 catcagaaac gccactctga aaacgacctc ccatatacat aaggtcaacc cagttgaaat
     8041 ttttcattaa tgtattacct gctttataac gtgccacagg atagcttctc aactgatcaa
     8101 ttgcagatac atttggactt ggatgtccgg gagcaaattc tgtgttatcc aaaccagcgg
     8161 atactggaga tggtgtaggc aaactaccat taaacgtcag attaaagcta tagcttttat
     8221 ttgtttgcac tgcatcttta taatgctgat aaattaattt agcaggaaca gttctgttgt
     8281 aaatagcaat ttcatcatac tcaccaaaat atttcatata gctaacaccc tgattccaga
     8341 acaaacttgt gtttgcacca ttcacaacct tacctgaaat atttttagaa tatcctgaag
     8401 gacattcacc atctatatat atttcttttt ttcctgtaag tgagctgtac tgaaatacaa
     8461 tatggtgcca gttgccatca acaaaatatc cataagagcg ctttcctatt ccgtcaaaac
     8521 taactttcaa ctcatcagat ccgcttccgg atgtataagt atagaagtat attccgtcaa
     8581 aattaaagtt agcggaaaaa cctccatcca ttcttctgaa cagcaaagtt tgattgaact
     8641 gataaccggg tttgaataaa aactcaatgg caaatgatgt atctgtcaca aaattgcctg
     8701 tgttaatatt atcaccactg ctacttagta ttaaaccttt gccaactgca ctgttatttg
     8761 aaacagtgta gccacaacca taggaagtaa aatccatatt ggtgcgattc attgagtcct
     8821 tgttaaaagg actgctgtta aaggtccagt agcttactgg tgcataggtt tgcgcatttg
     8881 caacctgaag caacgataat gctgcaaaaa gtaaatggat gaataataac gaaatgctca
     8941 gaatcttgaa ttttctgaac tgcctgttaa ttactcccct gatttctgtt gtcatttcgc
     9001 tgacagcatt tgcgaagtag attttttgtg ccatttgaat tttttttggt tcaacaaatt
     9061 tttggcacgt tgtttgaaaa gaagctttag aatgatttga ctttttttct cgtgccaacg
     9121 caggcaatat aaactgctgt tttaccttag ggtattcaac taaccttcat tattgcattt
     9181 gcaagaaaac gcactgtgtt ggcaattatt taatttttat gacattgctt ttagctctta
     9241 gcacaaaaat catcatgatt taatttaaga ttttcatgat gtaagatttt taagtttcat
     9301 gaaaaattct gatacaattt aagtcattat aaaccgctaa gtgaaaaata aactactatg
     9361 aatcatttat tctattgaaa aagatgactg attaccaata aacaataagt agttagttta
     9421 ttaagaccag tcacataaac tattaatcac tcaataaaac caactaaaat tatctgacaa
     9481 cagctatttg ctctttttct gctataccat aagtttcaat actcaaaaaa tacaaacccg
     9541 ttggaagttg ggatgtatta attttaacca taccattact ccattcaaaa ggggcaaaaa
     9601 ctttattgcc aaccggatta aatatttgta ttacgggtgt ttcatcagag tcaaactgaa
     9661 tagtcaataa atcttttaca ggattgggaa atatagaata atgataaggc tttacgtaaa
     9721 ctctttgtgc aggaaatgtt tctgatgtgc catcaaaatc tgtttgcatt aatctgtacc
     9781 atgatgttcc gctaaccggg tagttatcag tataactgta attttgtatt gaattgctat
     9841 ttccctgtcc atttaccgtt gctaattttg accaattgtt cccattaaca gatttctcaa
     9901 tggtaaaatg actgttattt ttttcagaag cagttgacca gttgagcttc actctgtttt
     9961 tttctgcttt tgcagtaaaa gataataatt caacaggtaa tgcacccgga tcattgaacc
    10021 caaatccaaa gctatgtgtt ccaccaaaag cccctcctcc cataaaattc atattacata
    10081 ttgttgcgaa tgaagtacaa caaccttgat gcatagttcc taaataacct ttaccaaaac
    10141 catctgaagt aatattaact tttgtatttg tatatagagg gttgtttgaa agtggtgtta
    10201 atgtaaaaca gaactcataa gctaaacaag aattaggtgt acctgtaaaa tcacctttac
    10261 caatgcctgc tgcatctata aattgcaatg tccaggtctt accgccattt gatggagttg
    10321 ctacccaatt acctgcattg taaaatccgg gaattattgg tgcaatctga ggattaagat
    10381 atcctgatgc aggaaaatca aatacaaatt ttttgacgcc ttcaactcct gtagtatttt
    10441 taaaaaaagt agttatgcaa acagtgtcaa caacgttcaa tggtgagaaa aataatggcg
    10501 cagtttgata aacaccgtta actttcaact gaaagaatgg ttttgttggc tcaaaggtag
    10561 gtgtagcaca cacacttcca tcaaatgaaa ggttaccttg tgtactcgga atatttacaa
    10621 aggcaacatt ggtatcgctt ccaaatgaaa tatcataagt aactgttgtt gctgcatcaa
    10681 cccagacata aagtacagtg tcatttgaag cattagctaa aaaatagtaa ttcggtaatg
    10741 ttgttattaa attggttccc agtgactggc acgacccaca agttcttgta gcaaatacct
    10801 tgaatggacc agtacagttg attacttgaa caaaaatttg acttgctcca ttagtaatgt
    10861 ttactttata aagcacatct tccccgacaa tgttacatgg tggggcaagg tcatctccca
    10921 tacctgcatt agtctgatta aataatgcta cactaggaca aatagtaacc tccaggggat
    10981 tactacaatt gttttgtgct gttgcttgta taaaaaatac tatgcaaaca ataatggtgg
    11041 ttagacactt catttggttg gctattactt tggagttata cttattgcag acaatatcaa
    11101 cttgctttag cacacttctg ataaatgtct aaaccaaccg tttcaaagat tataccaaat
    11161 taaaaatgaa atttttcaac aagtattctg tttcttaata aaatactgta aaggaaatgc
    11221 gggcaatttg agaattgcta attggtgttt tgactatcag cacatcgcaa aaatcgttca
    11281 cagatgaact gaaagctata aaaattattt ttcttcaaca aaagaaaaca cattactatt
    11341 tactttgcaa aatcaaacat caacaatatt taattgatta aaaaagtgaa taaagaaatc
    11401 attcaaggta taagaaaaga attaaagaat atagcaaacg aagaagtgcg cattattgct
    11461 tcccgttttt ttaaacccgg tgaaatggcc aaagtatatg gtgtaaaatc accaatagtt
    11521 cgaacaattg gtaaaaaata tttgcagcaa gtaaggacat tgcccaaaaa agaaatattt
    11581 gtaatctgtg aagaattatg gcaatcatcc tatctggaag aatccgtcat tgcctgtatg
    11641 ttcactgaat caatgcataa aaaatttgaa gttggtgaca ttaaggtttt tgaaagttgg
    11701 ataaataact atgtcaacaa ctgggcatcc tgtgatactt tctgcaatca cacagtagga
    11761 gattatctga tgatgtatcc tgaaaaaact gagttattga agaaatgggc tgtatcatca
    11821 aaccgttggc tgagacgtgc tgctgctgta tctctgataa ttccggcaaa aaagggatta
    11881 tttcttcctt taatctttga cattgcaaat atcttactga ccgatgatga tgatttagta
    11941 cagaaaggct atggatggat gctgaaggct gcatgtaaac attatgaaaa agatgtgttt
    12001 gattttgtta tgttacataa atccaaaatg ccgagaactg cattgcgtta tgcaatagaa
    12061 aaaatgcctg agtcacttaa atttcaggct atgaaaaaat agcctcactc ctacactgta
    12121 caaaaatacc atcaacaaca ttatctttta ttcaaaagga ttaatctatt ttcgtagcgg
    12181 ttatttgcgc ttttattaaa aatgaaattt acttttcaac tttttctatt gctgcttttc
    12241 acagcaactc accttcgggc tcaggaagaa aatatcaata cagacagacc tgatcagagt
    12301 gatggtgttt atacattaac caaaaaacac tttcagattg aagaaggcgt tacaataggt
    12361 aaaaacctat ttgtaaataa tcttatgcta cgctatggac ttacaaactc aacagaaata
    12421 cgtctgcttt cagatgtagg aaagattgat gaatcaaatg gctttctgcc tttaaccatt
    12481 agtgttaaac aaaaaattat taaacaaaaa aacttgatac ccgcaattac agcagttgga
    12541 tatatgagct acgaaaaaat tgcatcaaaa gattttcaga ctaataatat ttcttatgaa
    12601 ctaaagttgg cttttgaaaa tgaagtgaat gaaaatttta cagtttcata caatgccggc
    12661 cttactgatg aatttaaaac ttttaattat actttaaacc ttggctactc accaatagaa
    12721 aaattatcac tctttgctga atatttcggt tcagtaaatt cttctgtaca gcataatgcc
    12781 gatgcaggta ttttaatttt gttataccct aaacttcagg ttgatatagc ctgcggtcat
    12841 tctattgccg atagtgataa tcggttttat actacctttg gcatatcata taaattttaa
    12901 tttaatcatg aagtatttta caactcacgc atttattctt ctgacatttt ttacatcaaa
    12961 tctttttgca caggacaact acataccggt taatcaaact ccggagcaaa taaaaaccta
    13021 cgttaaaaat cattttcctg caacgattat ttcttctgta aaaaaggaaa aagaattact
    13081 gaataatgag ttggaagtta aactaagtga cagcaccaaa cttactttca acaagaacaa
    13141 tgcattaatc gaaattgaat ccaacaaacc tataccggaa aaaattctgg cagacacgat
    13201 ttatgacatc gcgcacaaaa actatccaaa caataaaatt gttggatggg agaaaacaaa
    13261 acttggtcaa aaggtggaat tagataatga tgttgaactc tatttcgatg ataatggaaa
    13321 ttttatcagg acagaacatt aattcccgaa attactgcta cattttagtt gtaaaccatt
    13381 tattataaac acaaagtcag tttcttagag ttaaattata cacactccat acatctgttg
    13441 ttcataagat tgcatttcct gcattgctgt tttagctaat tttgcacagc aatttaagaa
    13501 acagatgcca agggataatt ccataaaatc agtactcatt ataggcagtg ggcctatcat
    13561 cattggtcag gcctgcgagt ttgactacag cggatcacaa gcttcacgtt cactgcgcga
    13621 agaaggaatt gaagtgacgc ttatcaactc aaaccctgcc acaatcatga ccgatcaggt
    13681 tacggcagat catatttatc tgctgccgct tactgttgat agcatagaaa aaatattaaa
    13741 tgagagaaag attgatgccg ttttgccaac tatgggcggt cagacagcat tgaatctttg
    13801 caaagaagcc gatgaaagag gcatctggac taaatatggt gttcgcatta ttggtgtcaa
    13861 tgtagctgca attgaaacta cagaaaatcg tgaatcgttc cgtaaactga tgatagaaat
    13921 tggtgttggt gtaccaccat ctaatgttgc caactcattt ctggaaggta aagaaattgc
    13981 acagcgtata ggatttccat tagtaattcg tccctcctat accttaggtg gaacaggtgg
    14041 cagttttgta atgaagaaag aagagtttga agacaggttg atgcgtggat tgcaggcttc
    14101 acccattcac gaagtacttg tagaaaaagc tgtcctcgga tggaaagaat atgaattaga
    14161 actgctgcgt gatgcaaacg ataacgtaat tatcatctgc tctattgaaa accttgaccc
    14221 tatgggtgtc cacacaggtg acagcattac cattgctccg gcaatgacat tgagcgatca
    14281 tacctatcag cgaatgcgcg atatggcaat taaaatgatg cgtgctatcg ggaaatttgc
    14341 tggtggatgt aacgtacagt ttgcagtaag ccccgataca gaagatatta ttgcagtaga
    14401 aataaatcca cgtgtttcac gctcatcagc cttagcttca aaagcaacag gctatcccat
    14461 agcaaaaatt gctgcaaagc ttgccatcgg ttatcacctt gacgaattaa agaatcagat
    14521 tacaggaagt acttctgcat tctttgagcc tgccttagac tatgttattg taaaaatacc
    14581 tcgttggaat ttcgataagt ttcatggtgc cgaccgcaca ttgggtttac aaatgaaatc
    14641 tgtcggtgag gtgatgggta ttggtcgcag ctttaacgaa gcacttcaga aagcatgtca
    14701 gagtctggaa ataaaccgtc atggtttagg tgcagacgga accggatggc ataaagttga
    14761 tgacatcatt tattcacttg agcatccatc atgggacaga atatttcatg ttaaagatgc
    14821 attgaaatta ggtcttccta ttcagaccat tcacaaccac actaaaattg ataaatggtt
    14881 tctgacagag attcagaaat tagtaggcat ggagcaagaa ctgagaagac atagtcttaa
    14941 gaatctttct ccggacttaa tgcttgaatt aaagcagaac ggattttcag attatcagat
    15001 tgcacatata ttgggaaatg tcactgctga agaagtttat gagacccgaa aagaaatggg
    15061 agtaaaacgt atttacaaaa tggttgatac ctgctctgca gagtttgcag caaagacacc
    15121 ctatttctac agcactttcg aaaggccggc acaagacagt aatggcaatg aaattaatgc
    15181 aagtgaaagt attgtcagta ataagaagaa aattttagtc ttaggcagtg gccctaaccg
    15241 tattggtcag ggtattgagt tcgactattc atgtgttcat ggtgtattag catcaaaaga
    15301 atcgggctat gaagcaataa tggttaactg taatcccgaa actgtaagta cagattttga
    15361 cattgcagac aaactttatt ttgaacccgt atatcaggaa cacatcttcg agcttattga
    15421 atatgaaaaa ccagatggtg taattgttca acttggtgga caaacggctc tgaaactatc
    15481 ggagtatctc aatgaaaaag gaataaaaat aatcggcact tcttatgaag acatggattt
    15541 ggcagaagac cgtggacgtt tttctgatct acttcatgat ttaggaatac cctatcctcc
    15601 ttatggtgtt gcagaaagtg ccgaagaagc attggttgtt gccaataaag ttggatatcc
    15661 ggttttggtt cgtccaagtt atgtgttggg tggacaagga atgagcattg taatcaatga
    15721 cgaagaatta gaacgtgcag taataaagct gctgaaaaat cttccgggta acagagtgct
    15781 cattgaccat tttcttgatc gtgcagaaga atgtgaagca gatttgattt gtgatggcaa
    15841 agatgttcag attataggta tcatggaaca tatagaaccg gcaggcattc atagtggtga
    15901 ttcttatgca gtgttacctc cattcagttt aagcgatgtt gtcatcagtc agataaaaga
    15961 ttatgcacat aaacttgcat tggcacttaa tgtaaaaggc ttattgaata ttcagtatgc
    16021 tgttaagaat gaaaaagtat atgtgattga agccaatcca cgagcatcaa gaacagttcc
    16081 ttttattgcc aaagcacatc aggtgccata cattaacatt gcaactaagg taatgcttgg
    16141 tgtaaaaaaa ttaaaggact tcaacattaa acctgctgaa tttggatatg caataaaaga
    16201 gcctgtgttt tcgtttgaaa aatttgctga cgttaataaa gagttaggcc ccgaaatgaa
    16261 atctacaggt gaagccataa gatttattga aagtactaat gatgcctatt tccgtaatct
    16321 atacaaggaa aaaagcatgt atttatcaaa ataattttac aatagcagat catgcgtgat
    16381 gtcttttata caatagttgt tgtctgggtg gtgattcaga tttataaaag ttttataaaa
    16441 cctaaaagca atacaactca aagaaatact accagacagg aaggcgatgt gacaataaac
    16501 acaaatacta ataacaacaa tcgcaacaac gaccaaggcg agtatgttga ttttgaagaa
    16561 ataaaagact gattttactc gtagcgcagt gctttcacag ggcttattcg cgaaacaatc
    16621 attgacggaa taagcatcac caatatacat gcaactaaag tacctgcatt aatcagcaga
    16681 atattagtaa ccgataaatt tattggaaca taaggaacat aatatgattc ctgtggcaga
    16741 ctaaccaaat gaaattggta ttgtaaccag cacaaaagaa gtcctgcaac atttcctgca
    16801 atcaatccaa tgccaacaat tcctgaagaa accatcagaa aaatttttct tattccgtca
    16861 tttcctgcac caatggcttt caacaaacca attgttgttg tattgtctaa taagataatc
    16921 aagagtgtag aaatcatatt gattcctgca accaatatca tcaaaacaag aatgatgata
    16981 acattgacat cctgcaagtc aagccaatga aatatttcag gataaattgt tttgatggat
    17041 tgcgcattca attcaaaacc tgcttcttta tagacacttt cattgacaga ttcaaggtcg
    17101 ctgagttgct ttaaatttac ttcatagcct ccggcctgca atggattcca gttgtttaat
    17161 ttctgaatta cagaaagtgg gcaaaaaaca tagaggttat caaactcttc aagccctgtg
    17221 gaataaactc cattgacttt aaacttgcgg gctcttggcg gctcatcaat aaaataaact
    17281 acaaaagaat cattcagctt taatttcatt ttgtctgcta cactttttga aattatagca
    17341 ggataaatac cagaatgtat ctgaagattg aaacttcctt caatcatatt cggattaaaa
    17401 aaacctgagt taaaagttga atctaatcct tttacagcta cacctaaaat atcttcagaa
    17461 gtgcggatta ttccggcctt ggtggcatag gcatttacac tgctgattct gttatcactc
    17521 tttagcttgt ttataaactt ctgatcaata tttacaggaa tctcctcgta ggattgattg
    17581 ttatcaaaat gttttattct gatatgtgcg ccaaagccaa caaccttaga ggtaatttct
    17641 tttttaaaac ctgtgactat tgcaacagca acaagcatca ccattacact tagtgcaatg
    17701 ctggttacag ctattgccag aacagtgcct gacatacgtt ggttgttaac actgcctgtg
    17761 aaaaaacgct ttgctatgaa gtaagagtat ctcaactaat ttattattct tgctgatatt
    17821 tcggtttcaa atttatgctt ttgaagcgtt ggatagtttt actatccata attatcagcc
    17881 atcaaagcac tgctcaaatg cttgacagtg taattaccgg tgccggacaa atcaataatt
    17941 atttgccgat catcagtgat aagaatgttg ctgtggttgc caatcagaca agtatagttg
    18001 gccaaacaca tttggttgac ttgcttcttt ccaagcatgt taaagtcaag aaaattttca
    18061 ctcctgagca tggttttaga ggcgttgccg aagcgggaga aaacattgaa tccggtaaag
    18121 ataaaagtac aggcctgccc attatttctc tttatggtaa tcataaaaag ccaacaactg
    18181 atgatttaaa aggtattgat attgtactgt tcgacttgca ggatgttggt acacgttttt
    18241 acacctacag ttcaacatta caatatgtta tggaggcctg tgcagaaaac aaaaagaaac
    18301 tgattgtttt agacagacct aatcctaatg gatattatgt tgatggccct gtgcttgaac
    18361 ctgaatttaa atcttttgtt ggccttaacc ctattcctgt tgtacacgga ctcactatgg
    18421 gtgagtacgc aaacatgctg aatggtgaag gttggcttgc caataaagag caatgtgatg
    18481 ttactgttat cagcgtcaaa ggatatacgc atgaaacaag atataaactg actgttaatc
    18541 cttcacccaa tttaccaaac atgacagccg tttatctgta cccgtccttg tgtttttttg
    18601 aaggaacaat tgtgagtatc ggccgcggta ctacctatcc atttcaggtt gttggctacc
    18661 ccggttttac tgagggcccg tttgaattta cacctaaaaa aaatccggaa atgaaacttc
    18721 ctccgctcta cgaaaatcaa aagtgcagag gctttgacct gcgtgatttt gggaactatt
    18781 acatcccatc acttaaaaaa ctatacttgt tttggattaa aaatatttac gacaactatc
    18841 agcaaaaaga taaattcttt aacgattact tcgacaaact tgccggcaca gacaagctgc
    18901 gcaaacagat aattgccggc ctgaacgaag aacagataag acgaacctgg gaagaaggct
    18961 tgaagtctta taaagttatt cgaaaaaaat atttactcta tcccgatttt gaatagcgat
    19021 tacaatttgg ttttttcaaa taaacaagtg gctttatcag tcatggtttt aacgctcggc
    19081 tattcttttg tatttttgca ctttatccac agtatttttg aaaatatata accatataga
    19141 ccattttgaa aaggtcagca atgccgtaat aacacttgga acatttgatg gtgttcatct
    19201 tgggcataaa gtaattattg aaagactgaa aaaaattgcc gtagatgtta acgggcaggt
    19261 tatagtactt acattttttc cgcacccccg aatggtttta tttcctgacg accacggaat
    19321 tcaactgctc aacacaattg atgaacgaac tatactgatg gagaaagctg gaatagatca
    19381 tcttatcatt cacccttttt cgaaagaatt ttcaagaatg agttctactg aatttgttcg
    19441 cgatatttta gtcaacaaac ttggtgtaaa aaaactgatc atcggctacg accatcattt
    19501 tggacgcaac agagaaggct cttttactga tttggaagaa ttggcaccgg tttataattt
    19561 ttctgtagaa agaattcatg agcaattagt caatgatgtt gcagtaagct caacaaaaat
    19621 cagaaacgcc ttgctggcag gcgatgtggc aactgcccgt gagtttttag gatatgatta
    19681 tgagttaaca ggaactgttg ttcatggcaa aaaattaggt cacagccttg ggtttccaac
    19741 ggccaaccta catatacgcg aggattacaa attggttccg gcaaatggca tttatgcagc
    19801 ccgaaccttg gttggtggtc aatggcgtga tggtgtgatg agtattggaa acagacctac
    19861 attcgacaat ggagcacgtt ctatagaggt aaatttattc gattttaatt ccgatattta
    19921 cggtgcaaag attaccattc gtatgtcgga ttttatacgg agtgagaaga aatttgaaaa
    19981 cagtgcagcc ctgatagaag ccatgaatga agataaattg gctgcaatta aacttttatc
    20041 gtaaaattta cgttaaacgg gtcatgaaac gcaacatcgt catatttctg attttgctca
    20101 tgacatctac agcacatgca caagagttgt gggacgatgt tgcaatagat tcattacctg
    20161 tatttcattc actgaatgca gctatgcaaa atccggattc agttttcagg cttgacctat
    20221 caaaacaaaa attgaaagag atacctgcgc aggtttttga acttacacaa ctgcgtgaac
    20281 tgaatgtcag cagaaatcat ttaacagaat tgccggagca agtttacaaa ctcaataacc
    20341 tgcaacgttt gtatgcaaac aacaacaaac taacagcatt gccacaacgc atcggaactt
    20401 taaaaaatct tcagacttta acacttgacc gcaatcgcat tgaagcattg ccaccggaaa
    20461 taggaatgtt gcataatctt gaagttttaa gtctttggga taatgaactg tctgaaatac
    20521 ctgatgaaat aaaaagtctt tcaagcgtat tgaaaaaatt agaattaagg ggaattcttt
    20581 ttaacgaaga gcaacaaaaa agaatacacg acttgttgcc ttatacaacc atctattttt
    20641 cgcctgcctg caactgcaag caataaacaa gtcagtctga ttatatattt ttgcctatat
    20701 gttacagaca gataaattac aattgcgtgc ctgcgaacct gatgacctgc cattaatgta
    20761 tcagtgggaa aacaactctg attattggct gcttagtgac actacaaagc cattttccaa
    20821 ttacaccatt tcagaattta tcaagcaaga tcagaacgat atttatacta caaaacagca
    20881 gcgttttgta atggaattac gtaaccctgt aaaaccaatt ggatttattg acctctatca
    20941 gtttgaagcc gttcacagac gtgcaggtgt aggaatttta attggcgacc tgagtgaaca
    21001 aaaaaaagga tatgctgcac aaagtcttga atgtttaaaa aattattcgc atacagtttt
    21061 gaatatgcat cagctttact gctacattca gtcatcaaat aaaaagtcat tagcactctt
    21121 tcaacaagca ggatttaaag aatgtggagt attgaaagat tggctcatta ataaaaataa
    21181 gcctgaagac gtagtaatgt tccaatttat attctgatta ctttttatta acagttgttc
    21241 gtcacaccca agcaaattga acttttttgt tcagttttgc tacgctacta aatcaaacaa
    21301 aatgcaaaca ttatcagaaa ccatcaataa ggtaaaggag tttcatgcaa ccttcggact
    21361 tcaatatttt gaaaagccac aagctgcaat agataaaaac attattgctc ttcgtcataa
    21421 gctgatgcag gaggaaaatg atgaatatct tgaagctgcc atgaacaatg atcttgaaca
    21481 aattgctgat gcactgggag acaagctgta cattctatgc ggcaccatca ttgctcatgg
    21541 attgcaacat aaaattgctg atgtatttaa tgaaatacat cgcagcaata tgagtaagct
    21601 tgacgaaaac ggaaagccaa tttacagaga agatggtaaa ataatgaaag gtaacaacta
    21661 ttttcttcct gatattaaag cagtattgga acagtgattc agaagctgag cattataatt
    21721 cccgtattta atgaatctga aacaataatt tctgtattgg aaaaagtttc tgatgttcaa
    21781 ttaataaaca acatcgccaa acaaataatt atagttgatg accattcaag tgataatagt
    21841 ttagaagcta taacgcaatt taaaaataaa catccacaag ctgatattac aattgttaaa
    21901 cagcaggtca atatgggaaa aggttatgct gtgcggacag gtatgagtaa agcaacagga
    21961 gattacatta tcattcagga tgcagacctt gaattaaatc ctgatgattt taatattcta
    22021 ttgaaaactt ctttagatca aaatgcacaa gtagtatatg gctcacggtt cttaaaaaaa
    22081 caacaatcac aagtgccttt gttgacttta atggctaata ggattttatc aatggtaaca
    22141 agcctgctta caggtaagaa agttacagac atggaaacat gttataaact aattgaccga
    22201 tcattactgc aaaacataca actcaaagaa aatcgttttg gatttgagcc tgagattacg
    22261 ataaagctcc tcaaaaacaa caacattcat tttgttgaag taccaatttc ttatcaacca
    22321 cgcacaaaaa aaatgggaaa gaaaattggt gttaaggatg gtatcagggc attatactgt
    22381 ttaactcgtt accggttttc atgaaaagag tttacatttt ttttgcactt tttgctttct
    22441 taacactgct tgcatacctc tggcaacaac gcacctttgg caatgaaaaa tatgagcgca
    22501 ttgttgatag tgatggcaaa ggttatgttg cttatttacc cgctttattt atttatcagg
    22561 atttaagttt tggtttttat tctaagccac agaatgataa aatagcccga tatttcaatg
    22621 atagattttt aacaaaacaa aaagacaaga cgattttaaa gacctatgtt ggtgtagctg
    22681 ctttaatggc acctttttat ctaaccggat gtctgacaca aataatgatt catggaataa
    22741 caaatggtta tgagccaatg gttcaattct ttatttctat ggcagccatt ttctatgctt
    22801 tggcaggatt atttcttatt tacaaaacat tgcatttatt gaattttagc aaaactatct
    22861 cagcacttac ttccacattg atttttgcag ctacaaatct ttgctactac accctcttcc
    22921 accctaccat gtcgcatgtg tattctttct ttttaatctc tgcatttgtt tatctatcag
    22981 tatgccttac aaaaaataca aataaaaaac atcttctact ttctgctgtc actttaggac
    23041 taattgttat cgttcgtccg ttaaatatta taatcatcct ttttccattg ttgctgttag
    23101 gaaaacaaaa acttactgat ttactaaaaa caaaaaccgg attgcttatc gtatctgcat
    23161 tattcttttt aatgattatt tccattcagc ctatattaaa ttatatacaa tgtggattgc
    23221 ttttcccttg gagttatgga gatgaaggat tttattttac caatccttca cttttgaaaa
    23281 tacttttcag tttcaggaat ggtctattta tatattcgcc actactgttt ctgtcaattc
    23341 cgggattatg gctgctacgt gatagttcca ttaaaatcac tgctgcaaca tcactattac
    23401 ttattatagt ttggtatctt ttggctgcat ggtggaactg gtcatcagag cctgcattcg
    23461 gcaacagaag ttttgttgac tggtatggct tgatgtcatt acctattgct gcatctattg
    23521 gctatatatg gaataaaagt aaagtctttt tatcaatact ctgttttggg tttacaggac
    23581 ttagcacaat acaaacctgg caatatctca atgacatcat tcatcctgac ggaatgaatt
    23641 tcgaaaaata tcgttatgtt tttttaaaga cttccccgga atatgttgcc tcagtaggaa
    23701 atggaaatga ggaagcttat ttaccggagc aacgactacc cttttttaca acaaaattaa
    23761 atcctgaaaa caaatcatca aacaatcata ctgaaacaat ctttgaattc aatgcacaga
    23821 agaataaagg agcacagttt tcaataactt tttcaaaaaa tcaacttttg cccatgaaag
    23881 ctatggtaac acttcagaga aaagaacttg agtttaaagc ttgtacacaa gctaccgttt
    23941 gtttcgatta tgctgattct ggtgggagtg tttattacaa tgaaaatttg agaatcaatg
    24001 atattccgcg taaaggaatc aatgaatggc gaacatttac ttatcagttt atcatgcctg
    24061 cactaaaaga ctccaattca caattaaact tttatatcaa taatcctaaa ggtgctcgtt
    24121 ttgaaatcaa gaacatagag ttaaaattat tcaatttttc aaacaagcaa tagtaagtac
    24181 atcaaaccgt tttaattatt ctatttttgc attcgatgca acgaaagttt atcaccaacc
    24241 ttgccttact gctgctgttg aatttgctca tcaagccatt ctggattttc ggcattgacc
    24301 gaactgtaca gaatatgatt ggtgcagaag gctatggaaa atattatgca ttattaaatt
    24361 tttcttttct gttaaatatt ctgctcgatg caggaattac taattacaac aacagaaata
    24421 tttcgcagca tcagcattta gtctctaaac atttttcggg aataattatt ctgcgatttt
    24481 tattggccgg tgcatacata ataatcagtt taattttagg ctttgctatt ggttattcaa
    24541 aagaagatat tcatttattg atattactct tagccaatca gttttttata tcatttatac
    24601 tatatctccg ctcaaacttg tcaggattac atctttttaa gcgtgacagt attatttccg
    24661 taaccgatcg ttttttaatg atacttatat gcggttatct gatttggtat aacaatggca
    24721 cttatttaaa catcgaaaat tttgttcatg ctcaatcggc ttcatattta atcacagcag
    24781 tagtagctat tactttttta catgggcgaa tcagttttca gaaattaaac tggcaacctg
    24841 cattttatat tgctattctt aaaaaatcac tcccttttgc actactcatc ctattgatga
    24901 cattctatag cagaatagat tcaattatgt tggaaagact tttacctgat ggagcatttt
    24961 atgcaggagt ttatgcacaa gcatacagaa ttttagatgc tgttaacatg tttggttttt
    25021 tatttgcctc cttactttta cccatgtttt cacacatgct aaaaaaacga caggatattc
    25081 aaccacttac aaaccttgca tttaaacttc taatgattcc tgcattgaca ttggctgcat
    25141 taggactgtt tttcagcaat gatattatga acttacttta tcacgaacat ggtgatttgt
    25201 ctgctcctgt ttttactatg cttatgttta gttttgtttg tatttcaaca tcatacatat
    25261 ttggaacatt gcttaccgca aatggaagtt tgcgaaacct taatcttact gcattaggtg
    25321 gaatgatatt aaacatttct cttaatctgt ttttgatacc acaattcaaa gcatccggtg
    25381 cagcattagc aggcttgatt actcaaatta ttatggcact ggcacaaata attctggtgg
    25441 caaaaatcct gcattggaaa ccggattgga aactcaacgg aaagttatta ttatttttta
    25501 tattgattgc tttagtagca gatttagcag tacgatatta tccggaaata aagacaacaa
    25561 ttgccattat tatactggca cctatccttg cattttcttt aggactactt cgcatttcag
    25621 agctaaaatc aataatgaaa gagcgacaat agcgaaacgc taatttgttt acatttgcgg
    25681 ctcttaaata aattccttca taatgaataa tgcccctgtt tacgaagaaa acttcgcatc
    25741 actaagtctg attcaattga ttaaaaaatg gaaaaagcaa ttaatgattg ttgcagtaat
    25801 ttctattgta ctgtcattta tttttacgct tccgtttttt attccaccta tgtttaagtc
    25861 aacggctatt gtttatccgg aaaacctaag tccttattca actgaaagtg ctacagaaca
    25921 gttattacag ttgcttgagt ctgatgaaat aaaaatggag ttgataaaag attttaaact
    25981 ctatgcacat tatgaaattg actcaaccaa gaaaaccgct ttaactaccg tttttaaaca
    26041 acttgcaggc aatttgagta tcaataaaac cgactacgaa tcagttaaaa ttgaaatgat
    26101 ggataccgat ccattgcagg ctaaacaaat ggccgattct ataatagaaa aaggtaacag
    26161 gcttgcaaga aaactttaca gagaaaaaac gatggaagct tacgtggttg ccaaaacaca
    26221 atttgatatg aaagaaaatg agctcgattc tgccgataag gttttaacag agttaagaat
    26281 gaagtccggt atcattgagt tctcacaaca gacacgcgaa atttcaagag catattatac
    26341 ttcaatttta aataaaagca ataatgtgcg tttggaagaa atgtttcaga aattgcaaca
    26401 atacggctcg gattatgtga cactcaacga gaaaatcaat tacacccgtg gagtttataa
    26461 caactacaag cagtatgtag aaaatgctga aaaagattta accaaagaac tttcatattc
    26521 aaatgtggta acgccaccaa tgattccgga taagaaagcc tatcctgttc gcagtttaat
    26581 aatgctgatg tttactgcct ctgtggtatt cttatctttt gtagtaatca tcatctacga
    26641 aaattctcag gaaaagaaac ctcaaacaaa ttaattttat gcaagccatt cagcaaaatt
    26701 cgcttcggtg gttttatggc cttacagcca tatatatgct aatggcagta ttcagtttgg
    26761 tgaatgaatt ttattggcta ttgattttgc ctgcgggcat tttaatcggg tactctgcac
    26821 tctatcatct tgacaaatta tttcttttta ctgcattctg cactccctta gcgattaacc
    26881 tttcaaaaac tgatttagga atcggtgtca gtctgccaac agagcctatt ttgtttggca
    26941 tgatgatcat ctatcttctt tcaaaattta ttgatggcag gtatgagaag tcctttatca
    27001 aacatccaat taccgttctc attctgattc atcttgcatg gatgctgatt acaagtttca
    27061 caagcagcat gtttcttgtt tctataaaac attttgtagc aagattgtgg tttgttgtta
    27121 ttggctttat gcttgcaaca tccatcttta aggatgaaaa gaaaatgcgg cagtttatat
    27181 ggttctatgc tgttggaatg gcaataacca ttatctatac aatcacccgt cacagcataa
    27241 atggatttac acagcaatct gcacactggg tgatgacacc tttttataat gaccatacgg
    27301 aatatgctgc tgtacttgct ttgatgctgc ctccgatttt tgggttgatg cttttgaaac
    27361 ataaatcttt tatgtaccgc ttttttgcat ttctgatttc aggattggta attgttgcaa
    27421 tagtattatc atacactcgt gccacctggc tcagtctggc tgtggctctt gtctctatgc
    27481 ttgcttatgt tttcagagta aagacatggg ctgtagtagc tataataatt tcaatggttg
    27541 tgggttttca gatttttcaa tcaacagtgt tatacaaatt cggaaaaaca aaggagtatt
    27601 cacagaatga ctaccgcggg catttaaagt ctattaccaa tataaagact gatgcatcaa
    27661 atgtggagcg tcttaaccgt tggagttgtg cctggcgaat gttcttagat aaacctgttt
    27721 tcggatgggg accgggcacc tatcagtttc agtattctgt ttatcaacta cagtttgaaa
    27781 aaacctacat cagcaccaac cttggcgata aaggaaatgc acatagcgaa tatctaggcc
    27841 cattaagcga acaaggtttt atgggaacta taatttttgt tgcattagct gttgtggttc
    27901 tttacaaagc atcaagagtc tttttgcaaa cgaattctaa caacatcaag gtgcttgcat
    27961 tatccatgca ggttggttta attacctatc ttgttcatgg atttttaaac aattttttag
    28021 ataaggacaa agcctcaatt ccattttggg catttgtagc tgctattacc gtaatggact
    28081 attatcacaa taaaaaacaa ctaaacccat caactgagcg agcgtaatga taagatggta
    28141 acatcatcgc taaaactatt gccctctctg aaatgatcca aatctgcaat cagtttttta
    28201 tgaaattcat caaggcttga attgttcata ttctgctcta taaataattt taacgcctcc
    28261 ataccataaa aatcaccctt aaggttttcg agctctgtaa taccatcggt ataacaaaaa
    28321 acagaatcac cactatttat ttttacatga ccttcgttta taaatggcaa ctcatcaaac
    28381 atgcctaaac ctgttgtgcc tctttctaaa agttcagact tgtttttact acaaaataca
    28441 ggaggattat gaccggcatt aacataatta atttcacctt ttttgaaatt tatcagtccg
    28501 ataaaaaagg ttataaactt ttcacctttt gcagtcttgc tcacacagtc atttaattca
    28561 gcaactaagt ttgtaagtac tggtctgttt tttattaatg catgcaggtt agcctggaaa
    28621 ttagacatca gcaatgcagc tgccattcct tttcctgaaa catctgccat gcaaaaaata
    28681 tgctcgtgtt tgttgagaga aataaaatca taataatcac ctcccacctg ttgatgtgga
    28741 agataatgtg ttgctatctc gaaatattca tttccacata agccaacggg aaataacatc
    28801 tcctgcattt ctgctgccag ttcaagttct ttgcgaaatc ctgcctgcgc aaccgcttct
    28861 ttatttaaac gtttattttc tattgctacc acaataatat ttgtaatggt atgaatgaat
    28921 ggaacaactt cttttattga aattgactga ctcgggtcaa ttgcaccaac aatgcaataa
    28981 gccaataaca tttcattgtg ataaaccggt ataaccgatt catctttttc aagccactgt
    29041 ggtgtggcta ctgtctttga tttagaaaat gatatttcat tgagaaacaa ttccggttgc
    29101 aaacgaccga gttcttcttt catgccatag gacagcacct gcttccaacc attctcattg
    29161 acaaacaaag aaagttttcc agcctttaaa tgatcatgca aaacattttc ataaatctga
    29221 tacaactgtt cagcaggcag gttgtaatta attgcttttg taatctgcaa aagccaatgc
    29281 aactgtactt tctttgactg aaggtcggca gcaagtttat cggtggtttc ttccatcact
    29341 attctttgtt atccaaaaac tcaaacactt caggcaatcg tttcatcaat gcaagttcac
    29401 gcatttttaa acgggcatcg gttgtgggac tgccgaagta agtcttacct gcatctaatg
    29461 ttttaccaac tcctgactgg ccgagcacca ctgctccttt gccaatagtc aaatctttct
    29521 gaacaccaac ctgtccccat aaaataacat catcttcaat agtgacaaca ccggctatgc
    29581 ccacctgtgc agcaaacaaa cagttcttac ctatcaccgt atcatggcct acatgcacat
    29641 gattgtccat tcttgttcca ctgccaataa cagtatcgcc tgacacgcct ttatcaactg
    29701 tacaacatgc tccgatctca acataatcac caataattac acgaccacaa gtatgcattt
    29761 tatcaaatcc ttcggggcgt cttttaaagt aaaaagcatc agcaccaata acactgttgg
    29821 catgaatgat tacaccatca cctatttcgc aatgatcata aataactaca ccgggatata
    29881 tcagacaatt tttacccact ttactatgag cgccaactac acttccctgc atcaacacag
    29941 ttccttcacc aatcacagca gtttgatcaa cctgttgcat actaaaagtt aatggtctgt
    30001 atttttttgt caacagatta taatctctga acggatcgtc tgaaataatt aatgctttgc
    30061 cttcaggaca ggaaacttct ttatttatca gaatagttgt ggctgcagaa tttagtgctt
    30121 tatcataata ctttgggtga tctacaaatg tgatatcacc ttttttaact ttatgtattt
    30181 cattgatgcc tgtaatctga aagccggcat cgccaatata ttttgcattc aggagtgaag
    30241 ctacatcagc aagcctgatt gctggtaata ttttcatatt tcaaactgtt tgactgcaaa
    30301 tgtaacgaat gtgttgtcat tagccatctg aattaccgca atcaatttat aaaaaaaata
    30361 acagtgttta acaaaaatat tttttcaggc atgtaacaaa tatgtttttt tgtacgtcct
    30421 atcttcaact atcaatcatt tgaaccggat taatttttca gctctaaaaa atttatgaaa
    30481 agatttattg ctctcaccta tgcacttctt tgttttacac ttgcatttgc ccaaaaacct
    30541 acacagacag tcagaggtac aataactgat cagcagagta aagagccttt aattggcgcc
    30601 tcgatagttt ggcttgactc ttcaacattc attggtgcta ctgctgatat aaatggaaat
    30661 ttcagactga ataacattcc tgttggaagg caagctttta aaatctcctt tctcggctat
    30721 cgtgaaaatg taatcaccag catcatcaca tcgggcaaag aagtggtttt aaatattgaa
    30781 ttggagcagt ctgtagttac tgcaaaggct gttactattg ttgctgaaaa acaaaaagac
    30841 aaacccaata atgaacttac tacagtgagt gcaagatcat ttacaataga agaaacttcg
    30901 cgctatgcag gcagtgttgg tgatccatcg cgtatggctg caaactatgc aggtgtaagt
    30961 ggtgccaacg actccagaaa cgacatcatc attcgaggta attcaccaac aggattgtta
    31021 tggagactca atggtgttga aattccgaat cctaaccatt ttggttcatt aggtactaca
    31081 ggtggcccta tttcaattct taataataat gttttggaca aatctgattt tcttacaggt
    31141 gcctttcctg cagaatacgg aaacgttctt gcaggtgttt tcgacctaca aatgcgaacc
    31201 ggcaacaatg aaaaacatga gtttctggca caggttggct ttaatggatt tgaagcaggt
    31261 gcagaagggc ctatcagtaa aaaacaaggc tcatcctatt taattaatta ccgctattcc
    31321 acacttggag tttttaatgc attgggaatt aatttcggaa caggcactgc aataccaaaa
    31381 tatcaggatg tttcttttaa cattaaaatt ccaaccaaca aagccggaaa attcacgtta
    31441 ttcggcatgg gaggtatcag tggtattgaa gttcttgaaa aagacagaga cactactcaa
    31501 aaaaatcttt atgtagatga taaaaggcaa aatggttact tcgacaatac catgggcgtt
    31561 gttggactga tgcatacatt ttttatcaac aattctacct acaccaaatt cagcctttct
    31621 gcatctattg cagggcaaaa atacaaagtg gacaccttgc ccgactttgt tgattacgct
    31681 gtgccctatt acagaaacaa gtccgaccag cagaaataca gtgctgattt cagccttaac
    31741 aaaaagtttt cttcaaaaga cttcctgaaa accggattca gcttttcgca atatcactat
    31801 acctatcgcg acagtggtgt ggtggacagt gtttgggaat cttacacaga ttataacggc
    31861 aatgcactac tcaccaaact ttatgtagaa tggcagcatc gtttttccga tcagttatca
    31921 ttgaacagtg gagtttacac acaactattt aattacaata atacttacag catagaacca
    31981 cgcattggag ccaaatggga aatcagcgat aagcaatctc tgagtttggg tacaggacta
    32041 cacagtcagt tacaaccaat gtacatctat tttgtaaaaa caatgaaaga tgatggcagc
    32101 tattacgaaa cgaacaaaaa tctaaaaatg actaagagct atcatgccgt gttggcttat
    32161 gacagaaata tttcaaaggt tttcagaata aaaacagaag tgtattatca atatttattt
    32221 gatgccccag ttgaaaaacg gcctacattt ttttcagtgt taaatgaagg tgccgacttt
    32281 ggcattgcag gaattgacag tttagaaaat aaaggcacag gttataacta tggttttgaa
    32341 tttactgctg agaaattcta cagtaaaggt tattatttcc tatttacagc cagcctcttt
    32401 gaatcgaaat ataaaggctc tgatggcgtt gaacgaagca ctgcatttaa tggcaatttt
    32461 gttttcaatt tattgggggg aaaagaatgg acaatcagaa aaaagaacac catttcaata
    32521 aatattaaaa caacctatgc cggtgggcgc agatatattc ctattgactt tgcagcatcg
    32581 cagattacag gagaaacaga atacgatgaa gaccatgcat ttgagcatcg tagaaaagat
    32641 tacttccgca ccgacctgaa aataggttac aggataaaca gaaaaagact aactcatgaa
    32701 attgccattg acctgaataa tgtcttcaac acacaaaaca tctggaatca gcagtatagc
    32761 gccaaaacaa acactgtaaa aacagagtat cagatgggtt ttttaccaat accgatgtat
    32821 aagtgtactt tttgataatg gcagctaaaa atatagtaca ctgccttcca aattttccat
    32881 gaataatctt taagctactc actgtaattc tttaatattg acctgccatt gattaacttt
    32941 gccgcatggc agatgtaata caattgttgt ccgatactgt tgccaatcag attgcagcgg
    33001 gcgaagttat tttaagacca tcatcagtta ttaaagaact gatggagaat gctgttgacg
    33061 caggcgcaac acgcattgat gtaagtatta acgatggtgg aaaaaacctg atacggatta
    33121 cagacaatgg ctgtggcatg agtgagactg atgcgcgtct ttgtttcgag cgttatgcaa
    33181 catctaaatt gcgcaaggct gatgacttat atgccatcag aacgatgggg ttcagaggag
    33241 aagcaatggc atctgtagct gctgtctgtc aggttgaatt aaagactaaa agagaagaag
    33301 atgaaaccgg aacacgcata gaaattgaag gctctgagtt taaaattcag gagccatgtg
    33361 tgatgcagtc cggcacaatt attaccgtaa aaaatatttt ctttaatgtt cctgcaagaa
    33421 gaaattttct gaaagcaaca ccggtagaag cacgtcatgt catggatgag tttctgcggg
    33481 tggctttacc gcgacctgaa attggattta gtcttcagca taatggcatg gaagtttaca
    33541 acctgccggc aacacatcaa cggcaacgta tatgtcaggt ttttggagca acttataatg
    33601 aaagattggt gcctgtagaa gaagacacaa ccattttaaa tattaaagga tttgtaggta
    33661 aacctgagtt tgccaagaag gtcagaggtg aacagtattt tttcattaac aaacgctttg
    33721 ttaaagatgc ctatctgaat catgctgtca ccattgcatt caatgagttg ttacctcaag
    33781 gcacttttgc atcgtacttt ttgttttttg aagttgaccc ttcgcgtatt gatgtcaaca
    33841 ttcacccgac aaaaacagaa gtaaaacttg aagatgaaaa atcggtttat gccatattgc
    33901 gctcttctgt gaaacgtgct ttaggcagat tcagtattgc acctacgtta gactttaatc
    33961 aggaacaagc ttttaatgtg ccgttatcgc agttaaacac tattcctgta cagcctcagg
    34021 tgaaagtgaa taccaattac aatccattta aaagtactga gataaataaa cctactcagc
    34081 aacaaaatgg gcagtgggaa aaactatatg agattgcaag tacgcccgat gtacaacaga
    34141 caaaactacc aatagctcct attgaagaaa ataatgcatc tgcatttatg caactgttca
    34201 acagttatgt ggcagtagca atgccagacg aaatgtggct tattgaccag caacttgcac
    34261 atgaaaaaat tgtgtttgaa aaaacacttt tacaaatgaa aggcacacct actgttatgc
    34321 aacaaagttt gtttccgcaa cagatagaac tcgacaacag acaggctgct actgtttcat
    34381 cgcttcttga agacctcaac cgttcgggtt ttgacttaca gccattcggt cagaatactt
    34441 ttgtacttaa tgctgttccc tcctatctag aaaccggaac agaaaagaaa attattgaaa
    34501 tgcttattga tgagtttatc agtggcgaag cttcggccta caatccaatg gagcgtgttg
    34561 ccattgctat tgcccgcagt gcatgtctaa aaagtggtaa agtgctttct gcaagagaga
    34621 tgtctgctct ttttaatgat ctgatgcaat gcgaaatgcc ctactttacc aagagcggta
    34681 aggcaataat ttttaaatta gaaaaaaatg aaattgaccg caagtttgaa aaaggttaat
    34741 gcttgtttaa accaaagaaa atatcatctg tttgatatga ttaaatacgg aaacctatct
    34801 tcgtaccgtt taacaatgga ttgccaaaat ttgtaatcgt tttttatgca ggaattccga
    34861 ccttcaagtt tcaaactttt acccgatgta attaaaaata ttatgatcgt cagtgcattg
    34921 gcattttttg ctgatttggc acttaataaa atcgggtttg atcttaatgc ttatgccgga
    34981 ctttatcttc cgtcttctga gcattttcat tggtggcagt acttttctta catgtttctg
    35041 catggaagtt tctcacacat tttactgaac atgtttgcct tctggatgtt tggcggcatt
    35101 cttgaaaatt tgtggggacc aaaaagattt ctgatttttt tcttggtaac aggacttggt
    35161 gcagcattca ttcacacagc tgttggatat tttgaattgc attcgttaca ggctaaagcc
    35221 gatgcttttt ttaatgctcc gggtgctgat gcttttagcg tgtttgtcag agaccatatt
    35281 ccctatcaat atctgaatgc cgattatgcc atggcagtgg aaaacattaa atcaaactgg
    35341 tatgcacatg gcgacagtcc aatgattatt gaacaggcgc gtcaggctat tgatgaatat
    35401 attcgtttta aaaccaatat acctactgtt ggtgcatcag gtgcagttta tggtgtgctc
    35461 atggcatttg gaattctttt cccaaacact ttaatttatc tttacttttt tatccctcta
    35521 aaagcaaaat atttagtact cattattggt ggcatagagt tgtattctgc atggcaaagc
    35581 aaccccacag ataatgttgc acattttgca catcttggtg gcatgctttt cggatattta
    35641 ctacttcgct attggcagaa aaataaccgg tcacaacttt tctgaaaatg cttcacaatg
    35701 tgaaaagtaa ttacaagtcg ctcgatatgc tgcaaaagat aattgtagtc aatgcaatta
    35761 tttatttgtt tgtaaacata ggcgatgttg tgcttagact ttttaatttt cctgatggca
    35821 cagtaagcgg ctttttaacc gactggcttg ctgttccttc ttctctcaag tcattgctgt
    35881 atcgtccatg gacattaatc acctattgtt tctaccacga aaactttctt catattctgt
    35941 ttaatatgtt atggctgttt tttatgggtc gtctttttac agaatacctt ggtgaaaaaa
    36001 aattattacc tctttatctt gttggtggct tatgtggctc tgtgttgttt attgtatttt
    36061 acaatctgtt tccactattc agtcgcgaag ttgatattgc agttgcctta ggtgcctctg
    36121 caagtgtgct tgctgttact gttgccattg caacactgtt accggattac gaagtaggtc
    36181 tgtttctgcc acagtggcgg gtaaaactga aatatatcgc cattgcactt tttgtaattg
    36241 acctgttaag tattacaggc aacaatgcag gcggaaacat tgctcacata ggtggtgcca
    36301 tctttggttt tatctatatc aagcaattaa aaaaaggacg tgatttaacc ggatggcttc
    36361 aacgaatgat tgatagaatt actcaaaaga aaaaaccatc aattaaggtt gtttatcgca
    36421 aagattcttc tgatgaatcg tatcacaaca aaaaagcaaa caatcaggaa gcaattgata
    36481 aaattctgga taaaataagc cgttcaggat atgcaagcct caccaacgaa gaaaaagaaa
    36541 tactttttaa agcaagtaaa aatcaggatt gatgctcaag tctgctctta acttgttgct
    36601 atggctaata accattttca ttgctgttgt aacttttatg gctgttgcag cggcatatat
    36661 tagtcctgca aaaatttggt ttcttgcact attcggtctg atatttattt atatctattt
    36721 ggtagcattt atctggatgt tggtcttatc attcagacat aaaaaaatga tgctgctgat
    36781 tttaattcca ttaatagcag gcttaccaac atttgttgca cattggaatt actcttcatc
    36841 aaaccaaaaa gcaacaggag aaaatacgat aaaagttctt tcattcaatg tgcgtgtttt
    36901 tgatctttac aactggacgc ataatctgca aacaagaagt aatattttta actttctgaa
    36961 aaaaagtgat gcaggtattt actgttttca ggaatattat acgagtaagt taatgccatt
    37021 taataacacc gattcattaa aatcattgtt aggaatatcc aatgtacaca cgatgctgcc
    37081 aatcatagaa cgcgattctg atgaatgggg cattgctaca ttcagcaaat atccaatact
    37141 gaataaagaa acagtattta cagataaaga atctgccaac ggatgcattg ctaccgattt
    37201 gctgattgga aacgatacag tgagagttta taacatacac ctgcaatcag tacgattggc
    37261 aaaaaacgac tatatttttg tgagtgagtt taacaataaa gacaacactc agaaaatgaa
    37321 aggctctaag caaattgttc aacgtttgaa aaaggcgttt ataaaaagag cgcatcaggt
    37381 ttgcaaggtt accactcata taggtgcttg cccgtataaa gtaattgttg ccggtgattt
    37441 taacgacaca cctgcatctt acgcctacaa ccaaatcagc aatggcttga cagatagttt
    37501 tcttagctgt ggaaaaggac tgtcctcaac ttatgcaggt atgtttccag gacttagaat
    37561 tgactatatt ttacacagta aaagcattaa aaccatagaa tatattcgac cattagtgca
    37621 tttatcagac cactaccctg ttatttctac cctaaatttt taacattttt atttatgaat
    37681 ttgtgaatta tttttttact tttggaacaa ttctaatttt cattgacata accaaactat
    37741 tttcactaac taaactgatt aacatttttc aattatgcga gtaaaaattt cattcacccg
    37801 cgacaatact tccggcaata gtattccatt gcatcatcaa aaactaattt acgatagttt
    37861 aaaaactgtt gttcacgaag ttggtggcaa tatgaaacag ctaaattttt cgtcattaaa
    37921 aggaacctca aaaattcaaa atggatttat gaagttttta tcttctaaaa ttactttagt
    37981 gatatcgggc agtaatgatg atttaataac aaaagtcagc aacagaatat ttgagaaaaa
    38041 ttttatgccc attggcagaa tgaatctgct tccaaaagga aaagaaatta cttctgaccc
    38101 tgagtttacc acgcagatgc gctatctctg tatatctcct ttagtacttt ttgatcctat
    38161 taaagatgca gcacgcagca ctgagcagat tgatcctaca tcacataact tttccgacat
    38221 attatttaac acaacacttg atgccatgga aaagtcaggt gtgcctgaag caactattag
    38281 ctcttatgca gaatttgacg tacagcctga tgcagagtat atgcaaaaag ctgcagcagc
    38341 aaataaaaaa tttgcccgtg tttacagaag tgctgaaggc aagaatatga ttggatattt
    38401 agttcctttt acactgcatg cacatcccga tgttcaccgt tatgtttggc ataatggcat
    38461 tggagtgatg acccatgaag gctatggcat gcttgatttg gcaaaataat ttactgaaat
    38521 cgtttttatt tataatttta gtcaggaata atattgctga tttcatgttg ctttgataaa
    38581 aaaatatttc ataattattc ttacagtttt cgttgtggta tttggcgcgg taatgctact
    38641 tcgggaatct attgtacgca ataaagtaaa ttctttttgt acttcattcc acaataaaac
    38701 cggtggcaac atcaacatcg gaaaaattca attcaatgga ctcagggtca tcgaaattga
    38761 aaacatcagt ttggtttcac cattaaatga cacactgttg catacagata cttttatggt
    38821 gcagctttca ttattaaagc ttattcgtct aaaaaatcct gtctccaatt tttcggcaca
    38881 tggcattaaa attaaaattg cctccggtga ttcaattagt aactactcct ttctttttag
    38941 aaaatcaaaa cctgaaaaca cagaaataac atcagtacaa agtacacagg cttcaaaagt
    39001 atttacactt tggaataagt tgacagagtt gtccgactta agttttgatt tggaacgatg
    39061 caaaattacg atcatcagaa ataaagaaac cagaaacttt tactttccgg aaattataaa
    39121 tcgcagaggg aaatgccgct ttatagctta cagcaacgac catttacaaa cagaaaaatg
    39181 gcaagccggc tttaatattg atgatgataa aaacgaaatg tcactaaaca tcaaaaaact
    39241 ggcaggagaa aactggcttc cattctttga tgttgacaac aaaccaaaga ttgcttattc
    39301 ggctatttca ctacaaatta aacaaccctc aagaaaggat acaacatttc aggttaaggc
    39361 agaacttgat agcctgagtg taaattattg gcgtattgcc aacgatgatg ttgatttcaa
    39421 taaactttcg ttttccggaa tttgctcatt caataatcaa cgaattgaaa ccgatagcgc
    39481 tgtaaacatt accatcaata aaatccattt taaaacaaaa gctgccttcg acagaaaaca
    39541 gcctcatagc tatgcacttt ctttagcatt tgacgaaagt gcagatgatt tttttgaatc
    39601 gcttcccaaa ggaatttttt atcacttcaa aaacttcaaa gctaaaggca gattaaaatt
    39661 tgatttaaac tttgcacttg acatgtcagc ccccgattct ctgatattta attcagactt
    39721 aacaggcaat gcattgacaa ttgaaaaata tggtgatgag aatttctcac gtataaacaa
    39781 cacatttctg tttgatgcct atgatggtga cagattgcaa cgaagttttc tgattgggcc
    39841 tgagaacagt gattttactc cactgaatga gatcagtcac tatctgcaat atgccgtcct
    39901 cacttcagaa gatccttctt ttttctacca tcgtggtttt attccagatg cctttaaaga
    39961 aagcatgatt gaaaacatta aagcaaaaag gtttgtgcgt ggtggaagca ccatcagcat
    40021 gcaattggta aaaaatgtgt ttttgtctcg tgaaaaaaca atttcgcgta agctacagga
    40081 gatgcttatt gtttggctaa tagaaaacaa aggattggtg agtaaagaaa gaatgtttga
    40141 aatttacctt aatgccattg aatggggtcc cggaatttat ggaataaaag aagcatcaca
    40201 attctatttc aataaacttc cttcacaact gaatttggca gaaagtattt ttcttgcatc
    40261 aataatcccc cgaccaaaat actatcgtta ttcatttgat gcacaaggta acttgaagtc
    40321 ttaccttgct tcctactata gaacggtgag caacagaatg ctttccaaaa catggatttc
    40381 accaatagat acattcaatc ttcaaccgca ggtgaaactt aaagggccgg cattaaaaat
    40441 tgtaatgcct gctgatagtt caatgacaga ttcactgcaa tggaatcagt cgaatgaatt
    40501 attttaaaga gactaaaggt acttagttat ttcaggactt gtgggcaaca cacttgagcg
    40561 aaaacgatga atcagttttc cgttttcatc aattaagaat ttttcgaagt tccattttat
    40621 gcctcctgta aaatctacat taggttgttc tataagccaa cggaaaagcg gatgcatatc
    40681 tctgccctta accgatactt ttgctgccat tggaaaagtt acatcataat ttttcttaca
    40741 gaactcagca atttcattgt tgcttccggg ctcctgccac agaaaattat ttgcaggaaa
    40801 tccaacaata accaatttat ccttatattt ctcatacagt ttttccaaat cactgtattg
    40861 cggtgtataa ccacatttag atgctgtatt tacaatcaga attttcttcc ctttatattt
    40921 tgagaagtca atctgccctc ctgaaagacc atcaacttta aactcataaa ttgaagtagc
    40981 agttgaagca ggaccaaaaa gaaatgcgaa cataatcagc aacagtttca ttgttttgta
    41041 attaacagat ttaaaacaac ctcttgtaaa aaaagtttaa caaagtgttc catataaatc
    41101 aaatgcctcg gcagaagtga tttttatatt ggcaaaatca ccaacacgaa cataattttc
    41161 attagcatcc actaaaactt cattatcaac ctccggtgaa tcgtattctg ttctgccaat
    41221 aaaaaagttc ccttccttcc tgtcgaaaag tgtcttgaat acatttccga ctttcttttg
    41281 attgatactg tcagaaatat tctgttgcac ctgcatcaac tcgtccaatc tgcgttgctt
    41341 agtggcttca tcaacatcat cattcaatgt gtaagcatgt gtattctctt cgtgtgaata
    41401 agtaaaaaca cctactctct caaatcgcat ttcttcaaga aaacgaatta atgtttcatg
    41461 atcctgctct gtctcacccg gataacccga aataaatgtt gtacgaatgg caatatccgg
    41521 aattttatta cgcactgtgt ctaataaagt aattgttttt tcgcgtgtaa ttcctctacg
    41581 catgctcttt aacatattgt cacttgcatg ttgaagtgga atatccaagt acttgcaaat
    41641 tttattttct ctttgcatta tatcaagcac atcgagcgga aatccagagg gaaatgcata
    41701 gtgcaggcga atccattcaa taccatcaac agagcataac tgctccatca ggtttgcaag
    41761 ttttctctct ttgtaaatgt ctaaaccata aaaggtagag tcttgtgcta taagcagtaa
    41821 ttcctttgtg ccttttgcag ctaagtgtct tgcttcatga accaactctt caattggttt
    41881 tgaaatgtga ttgcctcgca taagcggaat ggcacagaat gaacaaggac ggtcgcaacc
    41941 ttcagaaatt ttcatgtagg caaaatgacg aggtgttgta accaaccgct ctcctattaa
    42001 ttcatgctta taatctgctc ctaaagtttt tagcagcaat ggcaaatcgc gtgtaccaaa
    42061 aatattgtca atacccttta tctctttttc taaatcgggt ttgtatcttt cagaaagaca
    42121 tccggtgaca atcaattttt caattttccc tttctccttc tcttttgcgt aatgtagaat
    42181 tgtatctatt gactcctgtt ttgcattatc aataaatcca caggtattta taattacaat
    42241 ttctgattgt cttttcgatt catgttctac ctctatttca ttggcacgca actgacccat
    42301 caacacttcg gagtcaacaa cgtttttgct acaacctaac gtaacaacat taacttttaa
    42361 ctgtttggat gtttttgttc tcatagacta aaatgttttt ttaaaatcag attccggata
    42421 attgatttta accacatcat cattaatcag tgtattgaga aaatctatca gccttgcata
    42481 acttctcaca taggcatttg aagctttgct gaaatgctgt ttcaaccctg ttcttatttc
    42541 aagctcaatt actctcggaa aatgatcgga tgcgatataa ccgaagtttg cataatcatc
    42601 aaaatattct ttctgcagcg agtcaacagg gaaatctttc aaaaagtttc ttattgcttt
    42661 cttttgtttt gatttcaatt tctttgctat atatactaca cgcgattttc cataatcaga
    42721 aacaccactg attttcatgc tgtcatcctt taacaaatat tcataagagg taaactgcag
    42781 gtttctgtta tcagaaactt ttatacggaa agatttgtca atgcgctgtg cctgactgct
    42841 tatactaaac aaaacaaata cagcaagtaa taatttaaag tgagggtgca tgaaataatg
    42901 atattgagtt ctttataccg gtgcaaagtt atcaattctg cacttgcata gcaatgaaac
    42961 tactatttaa cctttgcact atgaaaattg cataaaataa aaaaaggatt gaaaaatact
    43021 actccgaaaa aaaatatttt tttttaacta cttcgtataa tttggttcat tacttttagc
    43081 attcaatttt gagaaccttt accagtatgt ctttccgaaa tattttactt gctgctgcaa
    43141 ttgtatttac atcatgcaaa caaaatgcac acttagtaaa aacagaaacc actacctacc
    43201 ctcttaataa accgggcttt actgaagttg attcctcaat cttaaactct tactcatctt
    43261 acaaacacac tattgactcc ataatggatc gtgtgattat tgtgagtgaa atgagcatgc
    43321 agaaaaaaac ccccgaaggt ttacttggca atttcacttc tgatgcagca ttaaaacaag
    43381 tagaaaacga tttccatgct aaagactttt ctaaaataga tttctgcttt ttgaataatg
    43441 gaggtttgcg tgctgcattg ccaaaaggag atattacaat tggcaacatc tactcactaa
    43501 tgccttttga aaatgaaatg gttttgctta cattaaatgg tgcaacaact ttaaaactta
    43561 tcaattttat tgctgctaaa ggtggtatgc ctgtttcagg attaacaatg cgcgtacatg
    43621 aatcaaccgt aaccgaagct aaaattcaag gaatgccatt cgacagtaca aaaagttatg
    43681 ttgtggctac atcagattat cttgccaatg gtggtgacaa tctttctttt ttagctgatg
    43741 ctgaaaagaa agtctctacc ggattaaaac ttcgcgacct gctaattcaa tatctggaag
    43801 aaaaaaataa acgaggagaa aaattatctg ctaaaattga agggaggatt atttatgaca
    43861 aatagaagag actttttaag aacatttgca gtgggcgctg ccggactcag tctcacagga
    43921 ttaccacttg aggctttggc taaaagaaga gttactaaga taactatact ccataccaat
    43981 gatgttcaca gccacataga cccatttccg gctaacgatc caaaatatcc gggacttggc
    44041 ggagtagcac aacgtgctgc tattattaat gatatcagaa ataaagaacg caatgtactt
    44101 cttcttgatg ccggtgatat ttttcagggc acaccttact ttaatttatt tggtggcgag
    44161 gtagaattaa aattaatgtc gcaacttgga tatgatgccg gtactattgg caatcacgac
    44221 tttgacaatg gtgttgacgg tctatcaagt cagttgaaac atgccaattt ccctctgata
    44281 atttgtaatt acgatttcaa aggcacttca atggagggca aaacaatacc atataaaatt
    44341 tttgaaaaag aagatgtacg tattggagtt ttcggacttg gcattgaact ggaaggcttg
    44401 gttgacaaga aaatgtatgg tgaaacaaaa tatcttgacc ccttggaaaa agcagcagaa
    44461 actgcgcatc atttaaaatt caatgagaag tgcgatttaa ttatttgtct ctcacattta
    44521 ggctatagat atccaaacaa aaaggtaagt gatgatgttc ttgcaaagaa atcgctcaac
    44581 atagatttaa taattggtgg tcacacacat acattcatag acaaaccctt tgtgtataaa
    44641 aatcgcgaca ataaagatgt tttagtggct caggtaggtt gggcaggaat aaaattaggc
    44701 agaatagatt tttttgttga taaaaaaatg cgtaaattga cagccgacag taccacgtta
    44761 aaagttttga attatgcaag agcaatttaa tttttttttc aagaatattc aaagcatatt
    44821 tgcacctcga ttaaaaaacg agaaccacca aaaaataatt tttgaatttt atcttacaat
    44881 tgaaaattta cttctatctt taaaaaccaa attttacctc aaaccaatac ttaactttta
    44941 aaattacgat tagtgttgaa acagacagag aaaaatgttg aaaaagtgtg ggaaaattgt
    45001 ttgaagctta tacgcgacaa tgtaagccca caaaatttta aaacatggtt tgaacccatc
    45061 aaaccggtta aattagaaaa gcatgtactg actatagaag taccaagcca gtttgtatat
    45121 gaatggctcg aagagcatta tatacaactg ttgcgcaaga gcattaaaaa agaattaggt
    45181 gctgaaggtc gcttggagta ctctattctg atggagaata acatgaacag ctcaaaacct
    45241 tatgcagtga aaattcctgc taaagggaca ggcaacttaa agaatccaca ggtagcaatg
    45301 ccggttagct taaacacagc tataaaaaat ccattcattg ttccgggact aaaaaagatt
    45361 gatgttgact cacaactgaa tccaaactat tctttcgaca actttattga aggtgattgc
    45421 aaccgtcttg caagatctgc aggttatgca gttgccaaca aacccggtgg tacttccttt
    45481 aatccattat tattatatgg tggaacagga ttaggaaaaa cacatcttgc tcatgccatc
    45541 ggtattgaaa taaaaaataa tttccagaat aagactgtac tttatgttcc tgccgacagg
    45601 tttatgaatc agtttgtgga tgcagttaaa aacaattcac caaacgactt tgtacatttt
    45661 tatcagatga tggatgtttt aattattgac gacattcatt tccttgcagg caaagaaaga
    45721 acacaagatg tattcttcca catctttaat cacctgcatc agaataacaa acagataatt
    45781 cttacatctg ataaagcacc tgttgactta cagggaatag aacaacgttt actatcacgt
    45841 tttaaatggg gtttatctgc tgacttgcag tcgccagatt tagaaacacg cattgccatc
    45901 ctgaaaagga aaatgtataa tgacggaatt gaattgccaa ctgaaattgt tgaatacatt
    45961 gcttatagta ttactacaaa catcagagaa cttgaagggg cactcatttc tatcattgca
    46021 cagacatcat taaataaaaa gcctatcact ctcgaacttg ccaaacaaat gattgacaag
    46081 tttgtgaaaa atactgtgca tgaaatttcc attgactata ttcaaaaaat agtttgtgat
    46141 tattttgaac tgcctttaga aacattaaag tcaaaaacac gcaagcgtga agtagtacag
    46201 gcacgtcagt tagcaatgta tttttctaag agtctgacaa aatcatcgct ttcagcaatt
    46261 ggcgcacatt gtggtggcaa agaccatgca acagttctac atgcctgccg cacagtgaac
    46321 aacctgatgg agactgataa aaaattcaaa ggctatatca gcgatctgca aaagcgaata
    46381 aatcttaatc acaaaaatta aggtgaaaat tctgatggtt tgcctcggca acatctgccg
    46441 ctcaccactt gccgaaggca taatgcaaca taaactcaat caggcaggta ttgctgcaca
    46501 ggttgattcg gcaggtttga taaactatca tacaggagaa ttacctgata aacgctcaat
    46561 agcagttgct gccaaatatg gcattgacct tactgctcaa agggcacgta gttttagcaa
    46621 agatgacatt aaaaattttg attacatctt tgcaatggat aacaaaaact tcgaaaacct
    46681 gatggaatat gctaataatg cagcagaggc aaaacgcatt catcttatat tagactatgc
    46741 aggtctgggc aaaaagattg ttcccgatcc ctactatggc gatcaaagtg attttgagaa
    46801 tgtgtatagc ttacttgacg atgcctgcca acgcattttg aaaaagtttg cttatgctca
    46861 atccgaagtt gtctgacaaa aaaacaaaag gaattttata ccttattcct actgcaattg
    46921 gcagtatgca ggcaatagat tatttgccac aaactgtaat tgaaataatt cattcattgg
    46981 atgagtttat agtagagaat tcaaaatctg caagacagtt tcttaaacat tgtcacatcc
    47041 caacaccaca acagcagctc accattcatg aactcaataa acatgaaggc aatgtttttg
    47101 aagcatcgtt tttcagcaat ctgctttccg gaaaaaatgt tggcctgctt agcgaagcag
    47161 gctgccctgc tgttgccgac ccgggtgcat taattgttgc cgaagcacat aaactacaaa
    47221 taaaagtagt tccgcttaca ggtccatcat ctatactttt agcattaatg gcttcaggac
    47281 tcaatgggca acagtttgct tttaatggct atctgccaaa agaaaaaaca gaacgttttg
    47341 ctgcaataaa aaaagcagag aaagattctg cacaacgcag acaaacacaa atctttattg
    47401 aaacacccta tcgcaacaat gccttgtttg atgatttact aaataatctg caaccttcaa
    47461 cgcgtctttg cattgcctct gcactgcagt gtgaaaatga aatgattaaa acacagcgaa
    47521 tttctgactg gaaaaaacaa agacaggagt taaacaaaca ccctgtagtt tggctatttt
    47581 tggcttaata tttattgaat ggttgcgctg ctgaattttc tactcattac cataatatca
    47641 tgctttgtag tgtcattgat tttactcatg gtaagtccac gcatccctgt tttttttagt
    47701 tatattgcca ttgcactgca tataattttc attacgctgt atttgattta tagaaattca
    47761 gtccatgaag aaagagtagt catgctgttt gctgcaataa tttgtagtgg catcataccg
    47821 tttacgctca ttgtcagacg aaattttccg ctaatattaa aattatactt tggtcttttt
    47881 atgctgagtt taccactgtt tatcatagca ccatctaaaa tgctcactat tctgagtatg
    47941 ggctatctga acacagaaaa tgcacatgaa atgcaccttg acaagaacta ttacctctct
    48001 aaagagcaag gcttaattaa acaaaaagat aatgaagcta cctataaagt tgtaaaaaga
    48061 atgggcatct ttaataaaac ccttgtgcgc aatattttta ttggttttgt gcccgattct
    48121 gcaaaaattt tatttttaga cgaaaaatcg gaaattcgcc tccgtgctta catgcacggt
    48181 aaaacagaaa ttgatagttt agatataaca acaaaaaccg tagcatcacg cgacaccata
    48241 ttaaaaatca ttaaaactca agataaatga cacgattcta tttatcagta cttgcaatat
    48301 tctcagtact gacaacatct gcacaacaaa tgtcaacaga tatttttctg acaaactttg
    48361 gcatgaaccg cgaagggcaa tattttttta cagctccgca gaacattaca catcgtccgg
    48421 gctacgacaa tcagccctcg ttctctaaag acggcagtaa agtttattat gtagcctatt
    48481 acgatacttt gcagagcgac atctatgttt atagtcttga agatagcaca aacacaaggc
    48541 tgacagaaac tcctgaaagt gaattttctc ctcgggttac tgctgatgat ttaggattta
    48601 ctattgtgag agttgatgca gataaaggtc agcggtttta taaagtgctg atggatggca
    48661 caaatgagtt tcaacttctt ggtgtatctg attctgttgc ctattactgc attgcaaatg
    48721 acagcaccta tgctgttgca gacttaaaca acaatgtaat ggaattaaat atttacgaaa
    48781 tgccgagtga acaatttatt ccattggcaa aaaatattgg tcgttgtatt gcagtcattc
    48841 cagacggtga taatgaaata agttatgttg acaaagcaga caccaatggc tatatgctga
    48901 tgtcattttc aattaacaca ggattgattg gaagtgtatg ccaacttaaa aaaggtgttg
    48961 aagattatgt atggacaaaa gatggtaaac tacttattgg ttacgaagga aaattattga
    49021 tgttcgacag aaacaagcct gataatggat ggactgagct agcagacttc agtaaatctg
    49081 ttggaaattt ttacagaatt gcaatcagcc aacaaggaac tcaacttgca ttggttgcct
    49141 atactaagga agataaaaaa caggatgatg ctaaagatga ttccggcaaa aaagacgaca
    49201 agaaaaaacg caaaaagaag aaagatgaat aaaacagtat tcgttgctga atataattag
    49261 cacttaactc ctaattcttt ctggacgtaa gtacttctat aacaaaaact aaccttctct
    49321 catgcaaact gattatatca tagtaggaaa tggtttagca ggtagtttgc ttgcctggca
    49381 tcttttattg caagaaaaaa atgtagttat tattgatgag ttcaacaaca ctgttgcatc
    49441 acaggtttca gcaggaattt ttttgccgat tactggaaga cgcattgtaa ggacatggat
    49501 ggctgacgag cttcttgctg aagtaaaaac catcttcacg caattaggaa aattaatcgg
    49561 tgaaacatat ttatatgaaa tgcctgtagt tgaaattccg gcttctgtta aagagtggaa
    49621 tgaatggcaa cagcgtgcat caagtcctga catcagcaaa tacatcagcc ggtttttagc
    49681 acctaattca atagaatctg ttcattgccc atttggagca atagaattaa aaaacagcgg
    49741 ctatgtaaac atttctaaat gtgttacctt tttaaggaaa ctttatttag atatggaaat
    49801 tatcattgaa ggaaaattca actttgatga tttacagtat tccgataaaa tagtttataa
    49861 agatattgtg gccgataaaa ttattttctg tgaaggtgct gccggtataa acaatccatt
    49921 ttttaattat ctgccctttc aactctccaa aggtgaaatt ctgacaatac gttgtaatac
    49981 actttcagaa gagcagattt ttaatcatgg tatttttatt ctgccaatag ggaatcagct
    50041 attcagagtg ggttcaactt acgaatggaa tgatttaaca acaaacacaa cagaaagtgc
    50101 cagaaataat ctatctgaaa agttgcagac agtattaaag tccgattttg aaattgttga
    50161 tcagaaagca ggcatcagac caagtgtaaa agagagaaga ccttttatag gcatacatcc
    50221 aaaacataaa aacattggaa tatttaatgg gctgggcact aaaggtgtga tgcttgctcc
    50281 ttattttgca aaacattttg cctggcatct tgcacatcaa aaaccgttaa tggccgatgt
    50341 ggatatagct cgtttttctt aaactatttt tctttcttac gtctttcaat ctcttgtgta
    50401 atcatactca attcgcgtcc catctgacca ctcatgctgg tattctcagc ggcacgtctg
    50461 aataaataag gcagcacaga aactacaggt ccataaggca cgtacttaca aacacagtaa
    50521 cctgcattag caagattaaa actaatgtga tcgctcatgc caagcagttg cgaaaaccaa
    50581 acacgctcat cagaatttgc aatacctttt tcttccataa gttttgcaag taaggcactg
    50641 ctatcttcat tgtgtgtgcc ggcacaaaca gcaaccatgt ctatattttc aatgcacaac
    50701 tttatagcct cattgtaatc gtgatcacag gctgctttat ctggctgtat aggagaagga
    50761 taaccattta ctgctgcgcg ttctctttct ttttccatat aggcaccgcg cactaactta
    50821 taccctactc tgtaattacc tgaacgggct tttgcaatat tctctctcat aaatgcaacc
    50881 ctgtcatgac gataaagctg aacggtatta aaaataagtg tcttctcttt gttgtatttc
    50941 aacatcatgt catcaaccat gttgtcaatg gcatcctgaa tccatgtttc ttctgcatca
    51001 ataaataaac gcacattatt ctctgctgct gtcttacaaa ttgattcaac tcttgctctt
    51061 aatctcaaaa attctgcctc ttcggcttct gaaagtaaaa ccttactgct gacacgctct
    51121 aacaaatcca tcctgcctac accggtaact ttaaatacag aaaaaggaat atttttcttt
    51181 ccgcttgcat gctttacggt tttaataatt tctgcacagc tatggtcaaa atcagattca
    51241 ttgtgatggc cttctacaga ataatccaaa attgagccta ctctatactt tccgagatta
    51301 tttatcgttt cagtacattg ctcaatggtt tctcctccac aaaaatgttt gtaaatggta
    51361 gctcttatca atggctttat cggcaaaccc atttttaaaa ctgcattggt gaatcccgga
    51421 ccaattttta cgagaaagtt aatattgatg agcctgaata accaataggc tcttttaaga
    51481 tctgcatcgc ttttataagc aaatgcaacc ttcagattgt tgaatgatac agatggcata
    51541 ttaaaaattc tttgccaaag ataattgaat taaaatatcc gtaaatctaa tttctgtaag
    51601 aggtaaatat gatattaatc aacagcttaa cactttgatt taaccttact ttaaccaatt
    51661 tgtcaaacta ttggaatgtg tttggaaagt tattcgagaa aaaagacaag aatggtttgt
    51721 ttatatgaaa gcagtacaac ctcactccat caattctttc aatttctttt ccaactcatc
    51781 cggtgaaagg ttggagtaaa actgtccgcc tgcagctata gaaatctgcc cgttttcttc
    51841 cgacacaact aacgctaaag cgtcactctg ctccgtgata cctaatgcag ctctgtggcg
    51901 cattccgtaa tgtgcaggca catctaaact ttcagtaaca ggcaaaacac agcgggcagc
    51961 acgaattctg ttgccactga tgattacagc accatcatgc aaggggctgt ttttaaaaaa
    52021 tatactctct aaaagtcgtt tggaaacttc agcttccata atatcaccgg taccttcaaa
    52081 caaattcaaa tcggtttttc tggtcactac tatcaacgct cctgtttttg ttttagacat
    52141 tgaaacgcag gcacgaacaa taggcattac acttaactca acaggttgtt gcagcttgaa
    52201 atttccatgc aacaggttct taaaaaaatc acctcttgta aaaatagtat ttgagcctac
    52261 aaaaattaaa aacctgcgta gttcctgctg aaaaacaata ataagtgcaa taactccaac
    52321 tccaataaac tgcccaaaca aagcaccaag caattgcatg tcgagtactt tcacaaaaag
    52381 ccaccacaga aaatatatgg taagaatgcc tatgaggata ttcattgcaa cacctccctt
    52441 tagtaaacga taaagtttat acagcaccca tgctacaatt aatacatcaa gtatatcaag
    52501 cagtcttacg gttaaaaatc ctatgtgaaa caactccatg cctgtcgcta tgattacaaa
    52561 taacagaagt ttatttcaac tttactgttg tttcataatt tttttcgcac aatgttgttg
    52621 acacttcatt ttgaactatt acctatagtt ctaaaaaata aaaatcattc tgtataaaac
    52681 taagcttgca atgcttttaa tcgctgcaac aaaggcggat gtgagtaata gaaaaataca
    52741 tacatgggat gcggagtaag atttgacaaa ctatctgaac tgagcttttt taatgcactt
    52801 gctaatgctt ctgcactgaa tgttactctt gcaaaattat ccgcttcaaa ctcatttttt
    52861 ctggaataga tattcatcaa gattcctgta attagtgata agggtgaata caataaagta
    52921 aatgcaataa gacttaaagc cagactgtat tctgttccgc caagagctaa agaaaaccat
    52981 tcattattga taaatacaga aagcagccac agcaacaaag cagtttgaag gattgaaaat
    53041 atcattgaaa acagtgtatg ctttttcttg taatgaccta cttcatgagc cagcactgca
    53101 accaactcat ctttcgaata attattaacc aaggtatcaa acaaaacaat ctttttcttt
    53161 ttgcctaacc cactgaaaaa ggcatttgcc tttgatgaac gtctgctacc atccatcaca
    53221 taaaggttag taatttcaaa tttatttttc tgacagtagt tctcaattgc agtacgtact
    53281 tcgccatctg ccaatggagt aagtttatta aacaatggaa gtatcaatga ggtgtaaaac
    53341 atattcaata caatcataaa aaccgcaaaa actgcaagca tgtaaatcca aaaattactt
    53401 cctgtatatt gataaaacaa aacaaacatg gccaacaaac ttccacctaa tgctcctgca
    53461 agaaaatatc ctttaatcct gtctgaaata aaaacagcaa gtgttgtttt attaaatcca
    53521 aaacgttctt caataacaaa ggttgaataa agtgaaaacg gaatgcttat taaatcactg
    53581 aacaaagcaa aagttccaaa aaacaacaag gtggaccata acaaatctgt agttaccgta
    53641 tgtgtccaac catcaagcca accataacca ccataaatca gaaaaaagaa agaaataaca
    53701 aacatgaccg tttcactaat cattccgaag cggcttttct ctcgctcata ttgctgtgat
    53761 tttttatacc gttcttcatc ataatattgt ttcagttcat cgggttgtgt ttctgataag
    53821 tttaataaat ttaaaataga caaactcata ctcaacagat aattcagcac cagtattgat
    53881 gcaattatcc acaacaatat ttcttttaat tccatgcttt tttattaaag ttatatccta
    53941 ttccccactc tataaaatcg gctcttgcaa tatgtgtttt caggttgatg caggcaaaca
    54001 atccatcata aaaacgatag cgcattccca gtctgttata aaacaaacca tctttcttcc
    54061 atgcattttt tgcataaaca cccatctgca atatgacaga aaaatccgaa aatattattt
    54121 catacgctcc tgcaacacct ccacgaaaat ttgatattgt tccatgtgtt ttcatgtctt
    54181 catacgcaag tctgtaatta attgtcgggt cataaaataa atcaacactt atgcctgccg
    54241 cagatttagg gctgtactgc cttaaaaatg caggagaaag agtccatgca taccaggtgc
    54301 ctccggcaag cggataaact tgtttgaaag cccatgctcc aaaaattgta aagcgatttc
    54361 ttttttcaaa attttcagtt tcttgcctga taatctcttt tgattcacca aaactacggc
    54421 tgtaatgaag ttgtgccgta attaaattta ttcccagatt tggcattgcc gcacttccat
    54481 ttgaaaaatg tgttaaacct atggcaggaa caattctgtc gcgtttagta agccaaaact
    54541 ccgagtgaaa gttaaatgca ataacagcat tcagatgcga tcctatagct acattctgat
    54601 aattatcatt tctgttaaac ggtttggtaa tatatcccaa tccccatccg aattgaaaat
    54661 gtaaaaatgg tttttctgat ttgcctaatg gaatgtcaat gtaaggatat actgtatgcc
    54721 catagcctaa ctgttcttta ttacccaatc cgaaaaaacc atatttaatt cctgtttccg
    54781 gaaaatgaaa atgctgatgc catgatcgtt tgccgtctgc ctgctgtgat aaggtaatca
    54841 tcataccacc aagatgactg tgttgtagtg gctgaacaga aggccggtgt gccataacaa
    54901 aaccataatg accctctgca cccagcatcc atgcaccatg tgtggcttgc tgcccatgtg
    54961 ctgcaacagt cagcaagaat aaaaatagtg tggacagaaa actaatgcct gctttatgaa
    55021 tcataccttt gcaaagaaaa tgcttttagc aatggaagaa aaattaaatg cttttaaaag
    55081 aatttgtaca atcatggacg aactgcgcac acagtgtccg tgggataaaa agcaaaccat
    55141 agaatcgttg cgacatctca ccatcgaaga ggtttatgag ttgagtgatg ccatcttaga
    55201 taacaattta gaagaggtta aaaaagaatt gggcgacatt atgttgcatt tgatttttta
    55261 ttcacgaatt gccgaagaaa aaaacctgtt taacattgct gatgtgatta atacagaatg
    55321 tgagaaactg atttcgcgcc acccacatat ttatcctgct gccgatggac aaaaaactgt
    55381 tgttgccgat gaagaagaag taaaacgtaa ctgggaaaaa ttgaaaatga aagaaggcaa
    55441 gatatctgta ttggaaggtg tgcctcgttc attacccagc atggtaaagg cgcagcgcat
    55501 tcaggagaaa gctcgtggtg ccggcttcga ctgggaaaaa ccggagcagg tatgggaaaa
    55561 agttgaagaa gagttcagtg aatttcagga agctgtctcc cgtaaaaatc aacaggaaat
    55621 gaccgatgaa ttgggtgatg ttttttttgc actgataaac tatgcacgtt ttcacaatct
    55681 gaatcccgat gaagcattgg agcgcacgaa cagaaaattt atttcgcgtt ttagatatat
    55741 ggaagacaaa gtcagagaat caggaaaaac tttacatgaa ctctcactgt cacaaatgga
    55801 tgtttattgg aaccaggcaa aaaccactga aatctgatga tgcatattga tattatcaca
    55861 gtacatcccg gtttgcttga agggcctttc agtcattcta ttcttaaaag agccattcag
    55921 aaaaatcttg ttgaaataaa tatcattaac cttcgagact atgctgccaa caaacataaa
    55981 agtgttgatg actatgcctt tggcggtggt gccggaatgg tgatgatgat agaacctatt
    56041 gaccattgta tcacagacct taaatcaaaa agaaattatg atgaagtcat ctacatgtcg
    56101 cccgatggcg aactactcaa tcagggaata agcaatagcc tgtcgttgaa aaaaaatatc
    56161 attatcctct gtggtcatta caaaggtgtt gatgaaagag tgcggcaaca tcttattaca
    56221 cgcgaaattt ctgttggcga ttatgttctt tccggtggcg aactacctgc agccgtactt
    56281 tgtgatagca tcatcagaat tattcctggc gttttgaatg atgaaacctc tgccctgtcc
    56341 gactcttttc aggataacct tttggcaccg cctgtttata cccgacccgc aaattacaaa
    56401 ggctgggacg ttcctgaagt gcttttgtcg ggtaatgaaa gccttattaa tgaatggcgg
    56461 catcagcaaa gcctggaacg tacacaaatc agaagaccag acctgtcaga tggcaatagc
    56521 aaataactgt ttttcaataa aatacaattt tttaaacatt ttttaaaaag ttagtataaa
    56581 tcttatcacc ctaaagcttt ttttgctatt attgcactcc caaaatttta tcaatcatca
    56641 aaatataaag cgatggactt agttaaatta gcagaaaagc agttaatacc ggtaaaagat
    56701 gctccggatt tcaaagccgg tgataccatt accgtattct ataaaattat tgaaggtaat
    56761 aaagagcgtg agcagcagtt tcagggtgtt gttattcaac gtaaaggaca tggtgcaaca
    56821 cagacattta ccgttcgtaa aatttctaat ggtgtaggcg ttgagcgtat tttcccatta
    56881 ttcagtccta aaatcaccaa gattgatgta aacaagcgcg gtgttgttcg cagagcaaga
    56941 atctattacc tgcgtaatgt ttcaggtaag gctgcgcgta tagaagaaaa aattgttaag
    57001 acagctgaga ccaagaaaaa ataaaaaagt ttcattttga tttgtaaaga agcctcgtaa
    57061 taacggggct ttttttatga gtacactttc ccaaagacta agtaggcgcg agctaagtag
    57121 gcgcgagtgt cctttccgct cgtgcctttt atccttgcgg cctctggccg tccgatagct
    57181 atcggacttc atagttcagc cagctttaaa tcggtaaatt gattagcatt gctattttaa
    57241 gtaggagcaa gcattctgaa ttatcaaatt atcaaattac ctaattttca aattctctag
    57301 gtacttcatc aatcagaaaa aatctgcatt cctctgctca cccccaaccc ctgaaggggt
    57361 gtctgtccgc taaacatatt tctatccata gcattaaatc tattctaatt agagtaaagc
    57421 ttcttttggt tacgagtgag tgcaagcact ctgcattatc aaattatcaa attacctaat
    57481 tttcaaatta tctaagtact tcattattca gaaaaacgaa ctcctttctc ctcggctcac
    57541 ccgtagccct aaagggcgct atccatcaat cttcgtttat ctattctgat ttttatttgt
    57601 tcaagtactt tatcaattga ttgcataacc tcagcatttg taaaacgcat aacttcaata
    57661 tcataatgtt gcaattcaga ggttctccct gcatcatatt ctttttgttt ttcatgaatt
    57721 tcaccatcaa tctctataac cagttttaat tcatgacaat aaaaatcagc aataaaaata
    57781 tctataggat gttgcggctt gaatctaact cctaatttat tttccttcaa atgactccat
    57841 aacaattttt cactttgtgt catatttgca cgaagccatt ttgcattagc aaaaatttca
    57901 ggcttggcgc cataaaacat ccttctatcc ataacattaa atctattcta attaaaaaaa
    57961 tatttgtgtg caggaaccct tcaggcattt tgcaaagagc aacgcctctt ttggtcacgg
    58021 gtgcgtgcaa gcactctgaa ttcttatttc ttaattctta actgctaact ctgaattatc
    58081 aaaaatctta ttccaacacc tccctgattt gaattttcaa gtcaggtaaa ttatttacaa
    58141 taatatccca aacaatatcg taatcaactc caaagtagtc atgaattaac ttgtcgcgtg
    58201 ttcctgctat ttctttccaa cttatatgag gatgtattgt tttaaagtca ggctcaattt
    58261 tttttgatgc ctcaccaatt acttcaagac tcctgataat tgctcttgaa agtacagcat
    58321 cgttaaaaac ggtattttgc gatttaccat tcgtgtgaaa aagaacaaat tctatttcat
    58381 ccaaaatatg tcttaacaat tccttgtttt caaaatacat actttgtttc ttttaaaata
    58441 taaggagcta tatatttatt caatgattga ggggttaata aatcaacttt acgtccaaat
    58501 ataccctcaa ggaaataggc aagctctaaa aaatttttat aatttttttt accttcatca
    58561 aactctacta atatatctac atcactgttt tcattctgtt gatttcgaac aaacgagcca
    58621 aataatccaa gttgctttac tccatacttc aacaattgtg atttgttttt atgcaggagc
    58681 aaaaaaatgg tgtttttatt tatagaatct gattccattt ttcaaaatta aataatttat
    58741 gaatcaaaaa acaatgtaaa atagttctgt gtacaaattg acctaagtag gcgcaagcac
    58801 tctgaattat caaattatca aattacctaa ttatcaaatt atctaagtac ttcattattc
    58861 agaaaaaatc tgcattcctc cgctcacccc caacccctga aggggtgtct gcccgataaa
    58921 catacttcta tccatagctt taaaactatt ctaattagag caacgcctct tttggctacg
    58981 ggtgagcgca agcactctaa attatctaag tacttcatta ttcagaaaaa atctgcattc
    59041 ctccactcac ccccaacccc tgaaggggtg tctgcccgca gttttggtgt atttattctg
    59101 atttttactt gttcaagtac tttatcaatt gattgcataa ctctaagtag gcgcaagcct
    59161 tctgaattat caaattatct aattttcaaa ttatctaagt acttcattat tcagaaaaaa
    59221 tctgcattcc tctgctcacc cccaacccct gaaggggagt ctgcccgcta aacatacttc
    59281 tatccatagc attaaatctt ttctaatttg agtaaatcct cttttagcta cgggtgagcg
    59341 caagcattct gaattatcaa attacctaat tcaatgcttc accaaaaaat tttgaatcgt
    59401 gcgctagcgc ccctttaggg ctacgggtga gcgcaagcac tctaaattat ctcagacttc
    59461 atcattcaga aaaaatctgc attcctccgc tcacccccaa cccctgaagg ggtgtctgcc
    59521 cgataaacat acttctatcc atagctttaa aactattcta attagagcaa cgcctctttt
    59581 ggctacgggt gagcgcaagc actctaaatt atctcagact tcatcattca gaaaaaatct
    59641 gcattcctcc gctcaccccc aacccctgaa ggggtgtctg tccgctaaac atacttctat
    59701 ccatagcatt aaatcttatc taatttgagt aaatcctctt ttagctacgg gtgagcgcaa
    59761 gcattctgaa ttatcaaatt acctaatttt caaattatct aagtacttca ttattcagaa
    59821 aaacgaactc ctttctcctc ggctcacccg tagccctaaa gggcgctatc cttcaatctt
    59881 cgtttatcta ttttaatttt tacttgttca agtactttat caattgatta cataactcta
    59941 agtaggcgca agcactctga attatcaaat tatcaaatta cctaattttc aaattatcta
    60001 agtacttcat cattcagaaa aaatctacat tcctccgctc acccccaacc cctgaagggg
    60061 agtcaacccg ctaaacatac ttctatccat agcattaaat cttttctact taaaaaatta
    60121 tttgtgtgca ggaacccttt aggcattttg caaagagcaa cgcctctttt ggctacggat
    60181 gcgtgtaagc attcttaatt cttaattctt aattcttaat tcactatcac cttcactctt
    60241 tctatttcac tccctcgttt taatatgcaa ttataaattc ctccgctcaa ccccaacccc
    60301 tgaaggggag tcaacccacc tacttcatct cttggagtga ggctcaccct cttaaactga
    60361 ctccacgcac tcacataatc cttatacacc aacttaccca ttacatcata aatctctaac
    60421 tcaccgctca tgctttttac atcaaactgc aaggtaaaat tcccatcact cggatttggc
    60481 atggcagcaa ctcccccttt aggcggctga ggggtaggca gccctaccgt tggcgcacac
    60541 aagcacccaa gcgtgctgtc gcactccaaa aaataattag ggtggttagg cactgtatta
    60601 aagtaataag cgggcacctg cacactgtgt tggttaacat tgcaacccat gcccaccaca
    60661 tcagggttat ctatcgtatg ccagtgcacc gtgccatttc ctgtgcttat gtaaaccttg
    60721 ccatcaggag ccaagcctgc attgctgaat aaagtttgta aagaaggaca agtcgggcaa
    60781 ttaggtggaa cagacataaa tccatcatac gtggccaccg tctgcacgct tgcagcaatg
    60841 ttggctgctg ttaaatcaaa ctgatataca taaaatacat ttgatacata cagcacatct
    60901 gagtttggtg aaaatgccaa gcccacatta taccctccga caaacgatat tgcaatatgt
    60961 accggattgc tcaactgccc tgtgcaacgg ttaaagtcaa atatatccaa ccctccattt
    61021 aaataaaagt atgcaaaccg actgccgtca ggcgaaaacc atgcctgtcc ggcatcttgt
    61081 ggtctaattg cccctatatt ttgtgaataa ggtcccagaa tcaggttatt ggtggttaca
    61141 agaaagcgaa aaaatttatt ggtgtttatt tggtgtgtca taacccacca gtcccgtcca
    61201 ttggcatgtt tgcaggctgt tatttttccg gtaaccattg aatcggtaag taaaatctga
    61261 tttttagata ttactgtacc caaacctccg tttaagctca tgtcaatggt ggttaggtaa
    61321 aaataaagag agtaagcacc gggtacaggg tgattatcca gcgtgttatg aaacatataa
    61381 taaattgaac tgcttccggg ttggcgtata atcagtgcac cttgcggtat ttgaaacccg
    61441 tctgaaaact ggtctgcata cgcacccgga tttatcccgc ttccattctg catggtatca
    61501 ttgctggcat cggctatgta atagccgttg gtgtaaaaca ataaattacc ttgtgcatca
    61561 cttatattgg catgggtatg tctaaagtcc atttccaaac tatcgtaatt aataattggt
    61621 attccattaa aaaaatcaaa cttagtgttg ccaaaaggca atcctccaaa gctggagtat
    61681 cccatcagcc agttattatt tattccctgt tgggcagtac taatttttac tgcaatcagt
    61741 aaaatgaata aaatgtattt tttcatttta taaaaatgcc cggtttaaaa tgagttatta
    61801 ttagttcctt atcacaaaat tgaattgtaa aaatacaatg taatcaagaa taaaacaatg
    61861 cagaatgtag atttcagaat tatcaaactt atcagttgtg gctgaatact aaaaaaacta
    61921 ttttcacaat tcattgttta tccgtcttaa taaaattcta aaatagaaat gataattgaa
    61981 atctggagcg acattatgtg tccattttgc tacataggca aacgccacta cgaaaatgca
    62041 ttaaaacaat ttgccggtaa gaataatata gagttgaagt ggaaaagtta tttacttgat
    62101 cccgaaactc ctcttgaatt tgaaaagccg cttaatgtct atgaatatct tgccgaaaga
    62161 aaaggcatca gccttgcaca gtccataaaa atgcatgaac atgttgtggc aatggctaaa
    62221 caagcaggat tggattatca attagacaaa ttagtaattg ccaatatggt taaagcccat
    62281 cgcgttattc agtttgctaa aaagaaagga cttgccgacc tgattgaaga aaatatgttc
    62341 aaagcctatt ttacagaggg gaaaaacctt gctgatgatg atgtattagt tgctattggc
    62401 attgcatcag gtttaacaaa agaagaagtt attactgcat tgacagatga tctttatgcc
    62461 tatgaagtag atcaggatat tcaggaagca agagcgattg gattaaccgg tgttccgttt
    62521 tttgtctttg acagaaagta tgctatttca ggtgcgcaac ccacagaagc atttctcaac
    62581 acattaaaca aatcctacaa ggaatggctt gcatctaatc cacaacccgg cttacaaata
    62641 acctccggag aaagttgcag cacaaaaggt tgtgacaatt aaaacttttc ttcaagcaat
    62701 cactcttgta tgttgctgtc atgcaccaca tacaaaggtc tgtttctgat attggcactc
    62761 atgcggctga tgtattctcc tattattccg atacaaataa gttgcacacc tccaataaat
    62821 aaaacagcta tcataataga tgtccatccc ggaacataat ctttaatgat aaaccgcgag
    62881 taaagcgcat acaatatcca gataaaagaa ataaaagaaa ctacaaaacc actggcagta
    62941 gcaaatttta atggcgctgt cgagaaagat gtaatgccat caatagcaaa gcgcatcatc
    63001 tttctgtaag tatatcctgt ttcacctgca agccgctctt tgcggtcata ttctacatag
    63061 gtctgccgga aaccaatcca cggaatctgt ccgcgcaaat atttctgctg ctcaggcatg
    63121 tgttttagca cgtcaacaat tttacggtcc ataatgcgaa aatcgcctgt gtcaacagga
    63181 atttcaacag aggtaattcg ggcaagaagt ctgtaaaaaa tccttgccgt aaattttttc
    63241 ataaagttct ccccttctct gcttcttctt tttgcataaa caacctcaaa gccttctttc
    63301 attttctcat acatgt
//
LOCUS       DAOOOI010000006        62050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_64_length_62050_cov_28.351173, whole genome shotgun sequence.
ACCESSION   DAOOOI010000006 DAOOOI010000000
VERSION     DAOOOI010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 62050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62050
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64_length_62050_cov_28.351173"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1213
                     /locus_tag="PKX59_02045"
     CDS             77..1213
                     /locus_tag="PKX59_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89677.1"
                     /translation="MQFKEVVGQKDTKQLLLKMAGENRIPHAMLFMGNTGSGTLPLAI
                     AFAQLINCENAATDDSCGTCHSCRMYAKLTHPDLHFALPVNTIDDIKKPKTDDFIEQW
                     RTACLKNPYLSLQDWYDTIGIEKKQGIIATEESTAIVHKLYLKSFEGKHKVLIMWHAD
                     RMNSATSNKLLKIIEEPPGNTVFILITERYDGILPTILSRTQLIKIPIIDNDSLIKYA
                     CENYNLDINKARNMVHLSGNDLTELQALMSQENLSGQMELSFIEWMRMCYKFGINYKG
                     LYSWIEDISKLSRDAQKNFLNFSLGTVRECLITNMSGINHSRYTDATFNGFEKFSKMI
                     NAENIEPFAKTLEEAIYHIERNANPKILFLDLSFRMHRILHMKF"
     gene            1221..2408
                     /gene="ricT"
                     /locus_tag="PKX59_02050"
     CDS             1221..2408
                     /gene="ricT"
                     /locus_tag="PKX59_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOZ89678.1"
                     /translation="MNTETINTNTKSTGCTSCGTGSCNKLNVYDWLSDMEFPPGHIPF
                     NVVEVQFKGTRKEYFRNTDGHILKAGDIVAVEGNPGHDIGTVTMTGELVKLQLKKRGF
                     ESGAPEIKQLYRLAKQGDIEKWEQVKQLEHQTMHRARKIALQLGLKMKLSDVEYQGDG
                     KKATFFYTAEERVDFRELIKKLHEEFKVRIDMRQIGLRQEAGRLGGIGSCGRELCCST
                     WMTSFKTVGTSAARYQNLSINPVKLAGQCGKLKCCLNFELDSYMDALKDIPEQGTAIF
                     TEAGKALHRKTDIFKRIMWYELIPNLKDGEYYQPSADKWVMIPVDRVKEIIALNKENQ
                     KPADLKSGLPVEEEVEEPDYADMVGQDSLTRMDKKRKKKKKKKKPSQPNQQTNNGQQK
                     KPS"
     gene            2383..2865
                     /locus_tag="PKX59_02055"
     CDS             2383..2865
                     /locus_tag="PKX59_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOZ89679.1"
                     /translation="MANRKSLLSYLIASVLILVSASCNKQYHYEENKSIKGSNWDFSE
                     PISFDVNIDDTVTAYNMYINVRNQGNYRFSNLFLFVNTHLPQGQHLRDTVEITLATPD
                     GAWLGKGIGDLYSNRYLFRKNFRFPQKGNYHFELIQAMRVNPLPGIMDAGIRIENTKP
                     "
     gene            complement(2952..3794)
                     /locus_tag="PKX59_02060"
     CDS             complement(2952..3794)
                     /locus_tag="PKX59_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89680.1"
                     /translation="MHKRYIYLTSVIAGLLLTGSCSNKSGEENKEQTTETKDTMPVSE
                     VATFQFTYTIANLPPPMQVLDEFSKSKLEVNTALLNSADNADKYMTSLKQAMNYGIYG
                     VDLGYLVVNNRTLDAIKYYNTAKNLAAQLGMEETFNQFVNRFETNSNNRDSLTRVIDD
                     AYSATDAYLRSNKRLETASQVLAGSWLECQYLTVKSLLTATRNADNEILYKRVWEQRL
                     YLDNITKLLSEFADSDDSKKIKSDYEALLAIYKEPADEKQIDNAFLTRLASGLEKARQ
                     NIVE"
     gene            4052..6307
                     /locus_tag="PKX59_02065"
     CDS             4052..6307
                     /locus_tag="PKX59_02065"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ89681.1"
                     /translation="MTTKSFWSQRAMLSIRSNKRLFLGCLLAGSVGASAYLFLMPKVY
                     ESQAILKNINPDKQSTAIAAEMIRSPKTIQQSVKGMGLNVEYFVTKNFQTSECFDNKP
                     VNISYKLKDESFSQQSFSLQRDGEKTYILEYDDQAGHQQKRGSYNELLDLGSLAVTIE
                     KTPYLNLLDDKDEVSFTIYSDSRLANKIAEQNLSCSIEDGGVIKLNSQSANPAKAYRI
                     ASAVSKGYGNAGQQANESHSLANVQLINNQLDRVSQELDNAQRELSHFQSVNNAYEMP
                     QQAGAVLNTVSQLQIQKVEADMQLAALDNLSDYMRKNRNVNTISPEYGTITDIIYTET
                     YLKLNDKVAERQQLLEQGADLSKVDKEISSLKEMLAESIRNTRKKLTIRQETLAAAIV
                     SAKDRIQNLPQTEEKLQQLNRSIYLYTKLYDFLIQKRAEAMVEAPVLPPSSYILNEAK
                     MPTSPISPNAAKVWSLALLASLLFGALVTAVKPLLKIKISNRKDLQKFTDIPFIANIE
                     AGGKNHDFSESFMTLCTKILMLGNKQPVQMITITSAKAGEGKTTVAENLAKAMGKLGK
                     KALLIDMNPINPSLKNTLGNEEENSFAQVIDDDKNLHQAVQITNYENVDLLSAGYLAH
                     GVNTLLINDKTGNILADMKTLYDYVIFDTPETSNYMDAVPLMKMSDLNMFVIKANSAT
                     FDQLEYASQMKKDFQVENLFMIINGMTETMNHSGMQTSGKVKQIRRREQAEAEIRFMP
                     RMMKKAALWFY"
     gene            6318..7052
                     /locus_tag="PKX59_02070"
     CDS             6318..7052
                     /locus_tag="PKX59_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89682.1"
                     /translation="MGDKAKFLNHFFERISDIRYAAMGLTVSHPEEIAEDIIQIAINR
                     EDTRKILTVLEFSPYLKRVRLEEKPFYNFIHLKLSNGQWIQIQLIYGFVRKGIQFMNE
                     KEVLNECTINSGGIKVTSQHLNFEYVWLIHCLNKTNVPNHQHLFFSQYNRNERSAIFA
                     HIRSRYFLELNVLDELYTFHKKTYRQVYEKIIRRKENTWAKRNIRKTLYAFYSALNLI
                     KTKSIKVQFKGSGYHPFSKTERARVY"
     gene            complement(7049..7456)
                     /locus_tag="PKX59_02075"
     CDS             complement(7049..7456)
                     /locus_tag="PKX59_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOZ89683.1"
                     /translation="MNRPIRVLISKVGLDGHDRGAKVIASFLRDAGMEVIYTGLRQTP
                     QMVVNTALQEDVDVIGVSILSGAHLTVFPKIMELLKEKGMTDVLVTGGGIIPDADMKK
                     LNDMGVGKLFAPGTNTNEIVEYINQWVKQNRKD"
     gene            7582..8064
                     /locus_tag="PKX59_02080"
     CDS             7582..8064
                     /locus_tag="PKX59_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOZ89684.1"
                     /translation="MKKLVVQLFLLFSYGTTVAQDTNNNIPKPQSIIPQSGIFILHRK
                     IAIVEGPAWVTGDAAARVYCTQLKDSIGLHLAIVNAMREGTFENAINLKKINDAALGN
                     AGYRIKIKRPAIIVEANNDTGLTNALQYLFQIIPKKFFEDKNPKAKIPLSCSEITCKF
                     "
     gene            8334..9128
                     /locus_tag="PKX59_02085"
     CDS             8334..9128
                     /locus_tag="PKX59_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOZ89685.1"
                     /translation="MEIFGYACAILMGLSLGLIGGGGSILTVPILVYLFSIDVVTATA
                     YSLFIVGFTSLIGSFNHIRMGNVHLRTAVLFGIPSIIAVFITRAFIVPAIPQHLFNIG
                     TLQITKPIALLLLFAVIMLLASFSMIRKKKEKPDYDKVQYNYPLIIIEGFVVGTITGL
                     VGAGGGFLIVPALVLLAHLPMKKAIGSSLLIIATNSLLGFTVSLLHSEKTDWKFLLTF
                     SAIAIIGIITGSMFSKNVSNEKLKPAFGWFVLIMGVYIILKETLLK"
     gene            9227..9790
                     /locus_tag="PKX59_02090"
     CDS             9227..9790
                     /locus_tag="PKX59_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOZ89686.1"
                     /translation="MLELILKPWPWYVAGPLIGLIVPALLILGNKSFGISSSFRHVCA
                     ACMPSNIPFFKYDWKKERWNLFFVAGIMAGGFLAVNLLSASTKVDINPSLAASLNEKG
                     ITDFSGLVPADLFNWNSLFTLRGFIMMVVGGFLVGFGTRYAGGCTSGHAIMGLSTLQL
                     PSLIATCCFMAGGFIMANLILPYILSL"
     gene            9801..10313
                     /locus_tag="PKX59_02095"
     CDS             9801..10313
                     /locus_tag="PKX59_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOZ89687.1"
                     /translation="MKTEVTLEKEQNTDFETRSLDTICVNESHLEHSWEHNLKYSLMG
                     ILFGIILVKAEVVSWFRIQEMFRLQSFHMYGIIGSAVVVGALSVWLIRKFNIKTIYGE
                     EITLHPKEFNKGQIWGGLLFGLGWAITGACPGPLFATIGSGATVIVVSLLSAVAGTWV
                     YGKFRDRLPH"
     gene            10409..11497
                     /locus_tag="PKX59_02100"
     CDS             10409..11497
                     /locus_tag="PKX59_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89688.1"
                     /translation="MKKLFYILLFMTVGITTGFSQNLRKTNNAVLRFDSSHDKPIQNL
                     ATEFISNNTSKIQSTAPAFSFTESPYNHNMHITSDGTYYYTINGGSASNGQINKFDLN
                     GVLLQTFSIGIDGRGLSYNFTDGFLYASVYGGDIVKITDLVTGAFTTLFSGAMQNSQA
                     SFAISPDGTKFYDFFQGTLRIHDFTTGSVINTLSGLLYGLGNYGGEAAVAVDSSHIYT
                     WDAVTRNVYSYNLTGTLIQTLPLDSGDNGISLSIANGYLFVSKDANYAIGTWYGYSIS
                     SSTSITELQNNEPIIVYPNPTSGVINIDADIKTIEIFNVSGQKIFATTNINNLYQNKL
                     DLSHLSKGIYFAKLDGGDFLQTTKIIIQ"
     gene            11650..12321
                     /locus_tag="PKX59_02105"
     CDS             11650..12321
                     /locus_tag="PKX59_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011356388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOZ89689.1"
                     /translation="MISVSEIIIRLSLASFFGALIGLERERKHWAAGLRTHMLVCVGS
                     CLMMMVSAFGFSDILGTPDITLDPSRIAAQVVTGIGFIGAGAILFSKNETIRGLTTAA
                     GIWTVAGIGLATGGGMYLAAAVATAIAIIILWGLQPIEKMYFEKYKQKSLRITTSHDF
                     GDSEFLTALLKENGILLISLNFTRTNEYLIFQIQLEHADAGTIESVINILKKNSAIKE
                     IQWLN"
     gene            12484..12951
                     /locus_tag="PKX59_02110"
     CDS             12484..12951
                     /locus_tag="PKX59_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HOZ89690.1"
                     /translation="MINIGINKKQAKQLAAKLNLLLADYMVFYQTTRGLHWNIRGEKF
                     FELHLKFEELYNNLLVKVDEIAERILTLDETPLHTFSDYSKASQLKEIKNVSDPSRSI
                     KAILDAFETILIKQREILRMSADADDEGTNALMSNYISEQEKLVWMYSAYYNK"
     gene            12953..13270
                     /gene="trxA"
                     /locus_tag="PKX59_02115"
     CDS             12953..13270
                     /gene="trxA"
                     /locus_tag="PKX59_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOZ89691.1"
                     /translation="METTTQNRTFNDIINGSKPVLVDFFADWCGPCKMMAPILHEVKN
                     EVGEKAVILKIDVDKNPAVAAAYEIQGVPTLILFKAGKVLWRQSGVVNKPQLLRIIEQ
                     NIG"
     gene            complement(13321..14211)
                     /locus_tag="PKX59_02120"
     CDS             complement(13321..14211)
                     /locus_tag="PKX59_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HOZ89692.1"
                     /translation="MLTIKKLFFIGFTFITLHATAQDDLMKMLNESGEKEKKEEVTAT
                     FKSTRLINGHSVENNKQGVLQLMILHRFGQINSGGYEFFGLDNATMRLGFDYGVTDWL
                     NVGIGRSTFEKMYDGLLKVKLLKQRKSGTPVTLTAVASMQLNSLKFTDPQRKNYFSSR
                     LTYSYQLLAASKLSNAFSVQLMPTLIHRNLVPTANEKNDVFALGGGGRIKISKSIAFT
                     AEYYYVFPDQISSTYKNSLAVGFDIETGGHVFQIHFTNSRSMVEKGFIAETTGDWLDG
                     GIHFGFNLNRVFTVYYPKEQ"
     gene            complement(14222..14773)
                     /locus_tag="PKX59_02125"
     CDS             complement(14222..14773)
                     /locus_tag="PKX59_02125"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOZ89693.1"
                     /translation="MKKFILCVLMTGMYYTQAQNVFTTNAGTISFFSEAPLENIDATS
                     KKGHCILNVTTGDIVSLVAIRSFKFEKALMEEHFNEKYLESDKYKDAVYKGKIQEPLI
                     TDRDTSYQVTINGNITIHGIEKPANYKANYSFKGGVPAIEGSFNVALKDHNVEIPKLV
                     IQNIAEVVKVTCKFELAPYQKKD"
     gene            complement(14784..15155)
                     /locus_tag="PKX59_02130"
     CDS             complement(14784..15155)
                     /locus_tag="PKX59_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89694.1"
                     /translation="MNKSLILISGIFIALGISSCYYDVEDELYPNTGNCDTLNITYSQ
                     TVAPIISSNCKVCHSASMAQGNVVIDTYAAIKTYVDNGKLLGSLNHDSGYEPMPKNQN
                     KLSDCNIKKVSIWINQGAPNN"
     gene            complement(15162..15863)
                     /locus_tag="PKX59_02135"
     CDS             complement(15162..15863)
                     /locus_tag="PKX59_02135"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89695.1"
                     /translation="MNRTVFFWLMLLTIATSSCKHEPQDLAIATNDSTATEDCSNDTI
                     YFKNIIQPIINASCAYSGCHDAATAADGVILDTYANIIRTGKVKAGKPEDSKLYKSLI
                     ENDPDDIMPPPPGSPLSSVQINDIYQWIMQGAKNNECLGCDSVNVTFSGSILPIMQTI
                     CQTCHSGIAPLGSLSLVSYDDVSAVAMTGQLVGAITHADGYFTMPDKEDSTKYIPNCK
                     IKQIIKWVNDGYPDN"
     gene            16020..16295
                     /locus_tag="PKX59_02140"
     CDS             16020..16295
                     /locus_tag="PKX59_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOZ89696.1"
                     /translation="MNGMLLFLDNLGGGELLVIMVFVLFFFGSKKVPDLARGLGRASR
                     EFKDAMNGVQREIEQSMQTPPPSQQPQKNVENPVTPEKNGGVDEKTD"
     gene            16414..18603
                     /locus_tag="PKX59_02145"
     CDS             16414..18603
                     /locus_tag="PKX59_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOZ89697.1"
                     /translation="MSNVRFRALDATNGRIPVKITPPSVKVSDYFATNVFTKEAMFKF
                     LPKNAYNAVISAIETGEKIDRKIAGEIAHGMKEWAISKGATHYTHWFQPLTGATAEKH
                     DSFFDMSNDNSFEHFSGSSLVQQEPDASSFPSGGIRNTFEARGYTAWDPGSPAFIIES
                     PNGRTLCIPTIYVSYTGETLDYKAPLLKALHTLNEVAVPVAQYFDKDITRVSTTLGIE
                     QEYFLVDTSLFYARPDLAITGRTVFGHSPAKGQQLEDHYFGSIPDRVNAFMHDFEIEA
                     YKLGIPLKTRHNEVAPCQFECAPIFEEINLAVDHNQLLMDLMDKVARRHHFKVLFHEK
                     PYAGINGSGKHNNWSMVTNTGKNLLSPGGNPKSNLMFLTFFINTIKAVHEHADLLRAS
                     IASAANDHRLGANEAPPAIMSVFLGAQLSSILDNIEKKVGSSKMSVEDKTALKLNIGK
                     IPEILLDNTDRNRTSPFAFTGNKFEFRAVGSSANCSNSMAILNTIVADQLRKFRKEVD
                     ALIKKNVKKDEAILRVLRQYIVETKSIRFEGNGYSEEWVKEAKKRGLSNHQTTPVALD
                     AMVSKKSLKLFEDNHIFTHREAEARHEIMLETYIKKIEIESRVIGDLAQNHIIPAAFR
                     YQNFLAETVDNLKDCDLKSETYATQLQLIKEISAHVNAIKKNVDTMLASRDKGNKIAD
                     IRDKAIFYCDKVKVSFDTIREHVDALEMIIDDEIWPMVKYRELVTIR"
     gene            complement(18718..19293)
                     /gene="nadD"
                     /locus_tag="PKX59_02150"
     CDS             complement(18718..19293)
                     /gene="nadD"
                     /locus_tag="PKX59_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOZ89698.1"
                     /translation="MKVGLFFGSFNPVHNGHMMIANYFAEFTDLEQVWLVVSPHNPLK
                     PAGSLLNDYQRLHLVETAIGSYKKIKISKIEFGLPKPSYTINTLVHLNEKFPQHEFVL
                     IMGSDNLITFHKWKNFEQILENYKIYIYPRPDSDGGELKNHPSVKIIQAPLIEISSTF
                     IRQAIKEGNDVRFYLPESVYNYIDEMNFYKK"
     gene            complement(19294..20802)
                     /locus_tag="PKX59_02155"
     CDS             complement(19294..20802)
                     /locus_tag="PKX59_02155"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOZ89699.1"
                     /translation="MVCRILIAAFFLFLNSNIFAQTDSAVANGFKTFYYQNGNKSSEG
                     NFVNGKPEGLWKSYFENGKLKSEGNRHNNKPDGLWKFYNENGKITSELTYTKGLKNGL
                     QRYYFSDGKLKQEEEVKEGIRQGWVKDFGENGQVVNIIPFKNGVEDGVAKSYAEDGRL
                     ITITTYHEGYVRSEERINRIDKFGLKQGVWKVFFDNDKLKEETEYLDDKKNGFYRVYN
                     NDGTLIISERYKNGELIIDKKSTTKFEVKRDFYAGGKIKSIVNVINDKRQGVEKIYDE
                     KGNVTSGKLFEDDKPIAEGITDENGNRQGEWKFFYDDGSVHSIGKYKDGKRIDEWKFL
                     FQNGAVQQIGNYKNGLPEGEWKWFFKNGELRRDESFVNGKEEGISKEYNDSLQLIATG
                     KYVDGKKEGLWKYNNGEYIAEGEYADGLKFGTWHQTYKNGKTAFTGKYIDGLEDGEHI
                     YYNDNGKLHEVRQYKQGQRNGLWKIYDDKGVLILTINYQADKITRIDKIRIN"
     gene            complement(20792..21787)
                     /gene="nadA"
                     /locus_tag="PKX59_02160"
     CDS             complement(20792..21787)
                     /gene="nadA"
                     /locus_tag="PKX59_02160"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOZ89700.1"
                     /translation="MTEVSEIILKHGYLPESIPASMDLFEEIEKLKKEKNAVILAHYY
                     QESDIQDVADFVGDSLGLAQQAEKTNASMIVFAGVHFMAETAKLLNPDKKVVLPDLNA
                     GCSLASSCPPDDFKKFKEQHPGHVVISYINCTAEIKALSDVICTSSNVVKIVESFAPD
                     QKIIFAPDKNLGAYINKKIGRDMVLWDGSCIVHEIFSEQKIIQLKERYPEALVIAHPE
                     CEDHVLRHADFIGSTTGLLKYTKDNPAKAFIVATESGILYQMQKQSPEKMFIPAPPEN
                     NCACNDCPYMKLNTLEKVYLCMKNEVPEVIIPKHLQQAALQPLKRMLEISKQHGL"
     gene            22275..22706
                     /gene="rpiB"
                     /locus_tag="PKX59_02165"
     CDS             22275..22706
                     /gene="rpiB"
                     /locus_tag="PKX59_02165"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOZ89701.1"
                     /translation="MQSIIIGSDHAGFTLKEAIKKRFSSTFQITDKGTFSSDSVDYPD
                     YAHAVAGEVGNGKYTFGILLCGSANGVAITANRHSGVRAAICWMPEIADLARKHNNAN
                     IICIPARFVSDETAFLMIEKFFSTEFEGGRHQKRIDKINCV"
     gene            22725..23444
                     /locus_tag="PKX59_02170"
     CDS             22725..23444
                     /locus_tag="PKX59_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOZ89702.1"
                     /translation="MKFKFILLICVLPFFVNAQDEQDTSAVTSKKQKREIPRFAIKVT
                     GGLSWRLADVSPDENDLTKAYIKDLKSGNGFEISAKYFFNSKFGVGLKYNSHLSKVRY
                     DNMYFSYNNQNYFGTVSDDISINTIAVTFSGRIYNRQKTGYFTFDGGLGLMTYKDDTK
                     IATNKVNVKGNTLGFNISAGVGVNITRNLCLVIGFNFINGTLSQYDETENGVKKRVTL
                     NESEYQSLSHIDIFEGIVFTF"
     gene            complement(23447..24214)
                     /locus_tag="PKX59_02175"
     CDS             complement(23447..24214)
                     /locus_tag="PKX59_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019991162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOZ89703.1"
                     /translation="MNKIVKYIMLDILKSKVVIAYLVFLMVTGFSIFNLDENTSKGLI
                     SLLNIILFIVPLVCLIYATIYIFNSSEFIELLTAQPVSRKKLITSIYAGVVLSLTLAF
                     LIGIGLPILLFTPDATGFIFLLAGILLTLIFTSLAFLGSIVTKDKAKGIGVAIFIWLF
                     FTILYDGLLLLLMFQFGDYPIEKPLMILTTFNPVDLARILILIHTDYAALMGYTGALF
                     NEFLGSQKGTITILLILLIWIIVPTMIAVRKFRKKDL"
     gene            complement(24207..24920)
                     /locus_tag="PKX59_02180"
     CDS             complement(24207..24920)
                     /locus_tag="PKX59_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ89704.1"
                     /translation="MIETININKTFGKLRVLKDISIKLENNSSVALLGPNASGKTTFI
                     KSVLGMVLPESGTIKVNNTDIKNDDSYRSQIGYMPQIGRYPENMNIGQVFEMIMDMRK
                     EQMNHADTDLLDAFNMKSIFHKRMGTLSGGTRQKVSACLAFLFRPKIIILDEPTAGLD
                     PLSSEILKEKVKTEKNNGKLIIITSHILSELDELVSEIIFFREGELMFHKKLEELQLQ
                     TGEKSVSKVITTILKNNNE"
     gene            complement(24917..26176)
                     /gene="nosD"
                     /locus_tag="PKX59_02185"
     CDS             complement(24917..26176)
                     /gene="nosD"
                     /locus_tag="PKX59_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HOZ89705.1"
                     /translation="MKLNRALFVVVKCFLILLFFSGKSQAGVLKIGKKFSIKSIKEGV
                     AKAKPFDTLFIHSGLYKEQNILVEKTLSLIGVDNPVLDGENKFEVLTIDANSVLVTGL
                     TIKNSGYSSMKDLAGIKVMNSRQFLIKNNTLINSYFAIHIANCDNFRIENNTIRGTPG
                     TDEQSTGNAIHLWKCQYASIKNNDISGHRDGIYFEFVENSHIEKNNSYNNLRYGLHFM
                     FSNKDSYIDNKFTDNGAGVAVMFSNHISMYYNLFSMNRGASAYGLLLKEISDGDIKDN
                     MFENNTTGVLMEGTNRLHITKNSFYKNGYALRVQASCSNNTVHDNNFNGNTFDVATNG
                     SLVLNNFDGNYWDKYEGYDLKRDGVGDVPYRPVSLFSMVTENIPIASIFIRSLIVFLL
                     DRSEKIIPSITPENLVDNKPLMKPVAL"
     gene            complement(26163..26591)
                     /locus_tag="PKX59_02190"
     CDS             complement(26163..26591)
                     /locus_tag="PKX59_02190"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HOZ89706.1"
                     /translation="MRWTFIFISLLIVSCTTKPEPLVAGKDACVHCKMPIADTRFGAE
                     IISNKGRIYKFDDINCMLQWSRQSSNVDAGSAKLYVVNYFDQIDLMNAADAVYLKSDN
                     LRTPMNSGFAAFATKEEAEKLLKDDGGVLVDWNFVQNEIK"
     gene            complement(26604..27179)
                     /locus_tag="PKX59_02195"
     CDS             complement(26604..27179)
                     /locus_tag="PKX59_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89707.1"
                     /translation="MTKINGVSRVIIAISSLLIAISFFVPIWRIDLFAPQYPEGLVMK
                     IWLDKLTGDVEVINGLNHYIGMKKISVEMFPEFAFLKYIVAAFIVYGLVIAITGSRKI
                     LFSYLVVSVICGILALYDFYQWGYDYGHNLDPNAPIKIPGLVYQPPILGHKQLLNFDA
                     HSMPDVGGYIVIGSALICAIIWAMGLRKSKS"
     gene            complement(27217..27780)
                     /locus_tag="PKX59_02200"
     CDS             complement(27217..27780)
                     /locus_tag="PKX59_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOZ89708.1"
                     /translation="MNTKLLMGVMFAASVAFSCNPPAAENQQSTTTTEETQAGGQEAV
                     IDDDSQKDIVKIAVGSADHSTLVAALKQADYVTSLSNAGPFTVFAPTNEAFNKLPAGT
                     VDDLMKPENKAKLQDILEYHVSLGVFKPEMLTDGQTINQANLKNITISNKDNKIMINN
                     QANIIATIPAANGVIHVIDAVLLPPDK"
     gene            complement(27861..29783)
                     /gene="nosZ"
                     /locus_tag="PKX59_02205"
     CDS             complement(27861..29783)
                     /gene="nosZ"
                     /locus_tag="PKX59_02205"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HOZ89709.1"
                     /translation="MPSCKPKNVSTATSGDAAQKAYVAPGKYDEFYNIVSGGFSGQMS
                     IYGLPSGRLFRVIPVFSVDPEKGYGYSEESKAMLNTSHGFVPWDDLHHISLSETDGIH
                     DGRWAFGNANNTPRIARIDLKTFRTAEIIEIPNSAGNHSSPFITENSEYVVAGTRFSV
                     PMTNGTTDVPINTYKENFKGTVSFINVDKESGKMGIAFQILLPGVDFDLSRAGKGKSH
                     GWFFFSCYNSERANTLLEVNASKFDKDFIMAVNWKKAEEYVKAGKGVKKSVSYAHNTF
                     DDKTHTATSTIEKEVIMLDPKDCPDMVYLMPCPKSPHGCDTDPTGEYIVGSGKLAAVI
                     PVFSFTKMQEAINAKNFEGDYDGLPVLKYDAVLHGEVKKPGLGPLHTEFDANGNAYTS
                     FFVSSEIVKWRLSDLSVLDRVPTYYSVGHLCVPGGPTKKPWGKYVIAYNKITKDRYLP
                     TGPELTQSAQLYDISGDKMKLILDFPTIGEPHYAEAIPAELLSKNSTKIYKIEENHHP
                     YVTKGEKEAKVERKGNEVHVYMSSIRSHFVPDNIEGVKLGDVVYFHVTNLEQDWDVPH
                     GFAVKGANNAELLIMPGETSTLMWKPDRVGVFPFYCTDFCSALHQEMQGYIRISPANS
                     NVPLTFSTGTNQPPIN"
     gene            complement(29872..30384)
                     /locus_tag="PKX59_02210"
     CDS             complement(29872..30384)
                     /locus_tag="PKX59_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOZ89710.1"
                     /translation="MKSIQKHLINLMIAGMGITLFAACGGSAPKEEKAAESKEMNQYY
                     EEEKQKEDTKVADDGKGLGKFTDVKVDPKLDKQMADAGKSVYDVKCSACHKLTDEKLV
                     GPGWKGVTGKRSVEWIMNFSTNPDEMLDKDAIAKGLLEECLVRMPNQGLSDDDARHVY
                     EYMRENDGVK"
     gene            complement(30604..30771)
                     /locus_tag="PKX59_02215"
     CDS             complement(30604..30771)
                     /locus_tag="PKX59_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89711.1"
                     /translation="MLFAIFSLHNFREPGNLDAIDDRHCKKKDRLRALYIITGAIVVA
                     LLYTWLISVFC"
     gene            31101..32483
                     /locus_tag="PKX59_02220"
     CDS             31101..32483
                     /locus_tag="PKX59_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOZ89712.1"
                     /translation="MMKKTAVIFFILFSQLITVFGQSQDSLMLRKIYNESLEHGQAYN
                     YLKVLCKQIGHRLSGSPEAEKAVKWAEKSMKDFGADTVFLQNIMVPHWVRGEKESASI
                     ISSKNNQSTPLNVLALGGSVGTNGKTIAANVVEVTSLDELDKLGTSKCAGKIVFINQH
                     MDATKIMTFDAYGPAVTIRWAGAKRAAPLGAVAVIIRSVNPSLDDFPHTGTLHYPDTI
                     KKIPACAVSTNDAEKLSAMLKSDPQLKIKLKMNCMQLPDAPSHNVIGEIKGADANAGY
                     LIVGGHLDSWDTGEGAHDDGAGVVQAMEVLNLLKKAGIKPRHTIRAVAFMNEENGGKG
                     GEYYAKNALEKNEKHIAAIESDCGGFTPRGFFLQGDSLKKEKIRSWKNLFESYQIHSF
                     NFDGGGADIGHLKNQNTFLLGLLPDTQRYFDIHHSPNDVFEHVNRRELQLGAATMAAM
                     IYLIDKYGLE"
     gene            32483..33622
                     /locus_tag="PKX59_02225"
     CDS             32483..33622
                     /locus_tag="PKX59_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOZ89713.1"
                     /translation="MLLKETELKQNAAAYAELKKNISSETTPIFLDRKKEIFSDYDLA
                     TRRAAYYRWKSTESLDKLLIAFEANAIKAGIKVLWAVDTAQAHTELNAVIDKFQQHKI
                     HIHTCYNELETEKLNQPSPNSIKNKISICDAIFVSAEPAMIACLSDKNFYESDVKIIL
                     ASVDRLISSVNELELLMSLQSLQRNNQRESFSLISNAGNNSHKQEVYVLIFDNGRSKL
                     LESQTERQMLHCIDCGICQTVCPVTESIGTTGGNENNYGPPSYIRAVFDGGQREYGHY
                     AGLCNMCGKCNVHCPVHIDFKNSFLHLRHENVQQKHRSQKERLFYMVWKKTMLKREFM
                     NWKTVNPLRYAVETLFLKSKTGVRKLPQPAAKSFNQMWREKMGMK"
     gene            complement(33625..34662)
                     /locus_tag="PKX59_02230"
     CDS             complement(33625..34662)
                     /locus_tag="PKX59_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HOZ89714.1"
                     /translation="MQLRNLVSKQLLKKRLTEETFNRKTVSFYRYLKIEDVENFRNRL
                     YIDFEALNIFGRVYVAAEGINAQISVPEQNWELFKNYLEAQEQLKNMPLKIAVEDNGK
                     SFYKLIVRIKSKIVADGLNDNTFDVTNVGNHLSAKEFNEAMQQSGTIVIDMRNHYESE
                     VGFFKDALKPDADTFRDALPQAIEMTKDNKDNKILLYCTGGIRCEKASAYFKHHGYKD
                     VNQLYGGIIQYAREVNAEHLENKFLGKNFVFDERMGERISDEIVSTCHQCGGKCDTHV
                     NCANDDCHLLFIQCESCKATYEGCCTPECKAIINLPEEEQKRLRKGRVKKQAHAVYKS
                     RLRPDLKKILK"
     gene            complement(34698..34904)
                     /locus_tag="PKX59_02235"
     CDS             complement(34698..34904)
                     /locus_tag="PKX59_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89715.1"
                     /translation="MSALTFQNVNAQCSVCRAGAESSVEQGYKAGKGLNKGILYLMAV
                     PYVMVGVGFYFFYNKIKKKETKEG"
     gene            complement(34939..37986)
                     /gene="infB"
                     /locus_tag="PKX59_02240"
     CDS             complement(34939..37986)
                     /gene="infB"
                     /locus_tag="PKX59_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOZ89716.1"
                     /translation="MSDEKKDVRLSKATKDLGVGIDHIIEFLNKNGIQVEKNPNTKIS
                     ADAFSLLTKEFAPDMHARQEANEITREKQKRENTIIEVKDDSGKSGSESETDDELADK
                     LLSIKQQATEKQATAKAKETKEKTTKAKVVKAEEPAPVAEVDKPVETKEKAKEPEVVK
                     AKVSKLEGPKIQGKIDLEATEKKKKKTLKKEDSTAEEPKKTTAKKKAEVVAIEEEPKK
                     KKTTKKKEEEEVKQIEEVAAVTKEEEPIAIVETQPEVVIDATPVTEETDKTDTTEAEQ
                     KADDTTPPELFRTDREKLTGPTILGKINLPVDPPKKKPVATSSEDDRKKKRKRKDKKV
                     DIAKAGGPPHQGGAPRTGGGQRPGGGHGDFKRRNDFRNKPAEAKPEVTDKEIQDQIKA
                     TLARLSGGGKSKSSKLKKLKREQAQEELQSQLEQESNEKKVIKVTEFVTASELSKLLG
                     VPINQIISTCMSLGMVVSINQRLDAETLSVVAEEFGATVEFVSVEVQEAIKEVEDKPE
                     DLKPRAPIVTVMGHVDHGKTSLLDYVRKTNVISGEAGGITQHIGAYEVQLDSGKKIAF
                     LDTPGHEAFTAMRARGAKITDVAIIVIAADDSVMPQTIEAINHAQAAGVPIVFAINKI
                     DKPGANPEKIKEQLSKMNILVEDWGGKFQVQEISAKQGLNVDLLLEKVLLEAELLDLK
                     ANPNKNAIGTVVESSLDKGRGYIATVLVQSGSLKIGDVVLAGCYSGRVKAMFNERGNK
                     RNNAGPATPVLILGLNGAPQAGDQFHVMDDEREAREIANKRLQLQREQGIRTHKHITL
                     DEIGRRLAIGNFKELNVIVKGDVDGSIEALADSLLKLSTAEVQVNVIHKSVGAITESD
                     VLLASASNAIIVGFQVRPSVNARRLAEKEEIEIRLYSIIYDAINEIKAAMEGMLAPEF
                     EEKIVCNIEVRETFKIPKVGTIAGCMVLDGKMTRNTAIRVIRDGIVAYTGKLASLKRF
                     KDDVKEVSAGFECGLNIEGYNDIKVGDIIEGYEQVAVKRTLTV"
     gene            complement(38050..39288)
                     /gene="nusA"
                     /locus_tag="PKX59_02245"
     CDS             complement(38050..39288)
                     /gene="nusA"
                     /locus_tag="PKX59_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOZ89717.1"
                     /translation="MDHAVLIESFSEFKDSKNIDRATLMSIMEDVFRAMVRKKYGEDN
                     KVDVVINTDKGDVEMYRNRTIVEDGMVEDDNTQIALSDALKIESDFEVGEDVTEQILL
                     DDFGRRAVLAARQNLVARIMELEKESIYQKYKDRVGEIVTGEVYQVWKKEIMVMDDEG
                     NELSLPKNEQIPADFFKKGDTVRAVVLRVEMQGGSPKIILSRTAPVFLERLFELEVPE
                     VFDGLITIKNIVREPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDV
                     INFTNNLSLYIQRALSPAKISEIKLDDDTKKAAVYMKPDQVSLAIGKGGNNIKLAGKL
                     TGFEIDVYRDSDVEDDEDVDLDEFTDEIEPWIIEEFKKIGCDSAKDVLAISIDDLVKR
                     TDLEEETIREVVRILNEEFE"
     gene            complement(39301..39756)
                     /locus_tag="PKX59_02250"
     CDS             complement(39301..39756)
                     /locus_tag="PKX59_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOZ89718.1"
                     /translation="MIAEQIHELIKVYIADKDLFLVDLKVTPGKITVLVDKPAGITID
                     ECVLLNRYLYKEMESSGVFENKEFEVGSPGMDQPLKVIGQYKRRIGRPVKIILNDGKQ
                     HNGILVDANEQELQLLETIKEKIGGKKVEKQLTTTLPMQNIKETKLEFK"
     gene            39892..40614
                     /locus_tag="PKX59_02255"
     CDS             39892..40614
                     /locus_tag="PKX59_02255"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOZ89719.1"
                     /translation="MADNLNIEKVLFLDIETVPQFESYGSLNSADKELWDNKALHLKK
                     TDFDTAESLYPRAGIYAEFGKIVCISVGWFVIENSKRNLKVKSFYSDDETNLLLKFSE
                     LLKNQFNKSSHLLCAHNGKEFDFPYLCRRMLLNKIKIPDILNIGGKKPWEVQHLDTMD
                     MWRFGDYKSYTPLKLLTHIFNIPSPKDEIDGSDVWHVYWKDKDLKKIAKYCQKDVVAV
                     ARLILAFKNESVLNDSEIEILN"
     gene            complement(40611..41510)
                     /locus_tag="PKX59_02260"
     CDS             complement(40611..41510)
                     /locus_tag="PKX59_02260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ89720.1"
                     /translation="MELLNECPSCGTQDKIEYLTCKDFGYSGSYFTINQCVRCGLLFT
                     SPRPDEAEIGAYYKSDDYVSHTNKSKGIINKLYPIVRNIAVNQKVRLVKKLIPKNSYV
                     ADLGAGNGYFVKALLKAGFQTKGYEPSPEAIKLAKETFDTNLLPIESFYSSGNEEFNC
                     ITLWHVLEHVHTLKKTLNNIKLSLKKDGILIIAVPNPASKDAEFYKEFWAAYDMPRHL
                     YHFTPEALTSLIESFNFTLLDTKPMLFDSTYVSLLSERYKAKSSKPIQIIKAFYRGIH
                     SNMHAAIYTGNYSSLIYIFRKNG"
     gene            complement(41578..43452)
                     /gene="mnmG"
                     /locus_tag="PKX59_02265"
     CDS             complement(41578..43452)
                     /gene="mnmG"
                     /locus_tag="PKX59_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOZ89721.1"
                     /translation="MIQEYDIVVVGAGHAGCEAAAAAANMGSRVLLITMNMQTIAQMS
                     CNPAMGGVAKGQIVREIDALGGYSGIVSDKSAIQFRILNRSKGPAMWSPRTQNDRMLF
                     ASEWRQMLENTPNVDFWQDMVSALIIEDNKLKGVVTSMGHQVRCKAVVLTNGTFLNGI
                     IHIGEKRFGGGRSGEKAATGITEQLVSLGFVSGRMKTGTPPRLDGRSIDYSKLEEQKG
                     DENPGKFSFTDTIPLTTQRSCHITYTNDEVHNILKTGFEKSPMFTGRIQGLGPRYCPS
                     IEDKINRFADKDRHQLFIEPEGWNTVEIYLNGFSSSLPEEVQYAALKKIPGLENVKMF
                     RPGYAIEYDYFPPLQLNLTLETKKIEHLYFAGQINGTTGYEEAACQGLIAGINAHLKI
                     NNQNPLILKRSDAYIGVLIDDLVTKGTDEPYRMFTSRAEFRTLLRQDNADIRLTPVSH
                     QIGLADAQRFSRVTEKKQAVDRIIEACRTESISPSEINAYLNSKESAELSQKVKIIQV
                     LNRPGINLIDLINHSSTLSEKLSFISPELRNEVIEQAEIAMKYESYIAKEFEHTVKLN
                     KLENLILHEDFNYDKIKSLSAEARQKLSVVKPQTLGQASRISGVSPSDISILMVYLGR
                     "
     gene            complement(43539..43964)
                     /gene="ybeY"
                     /locus_tag="PKX59_02270"
     CDS             complement(43539..43964)
                     /gene="ybeY"
                     /locus_tag="PKX59_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOZ89722.1"
                     /translation="MQTEVNFFKEDVQFRLNDKDAISSWVVGCIKKHRKKPGTLNFIF
                     CSDELLYSMNIKYLNHKTLTDIITFDYTEDATISGDIFISIDRVRENAKLLKNSIKDE
                     LHRVMIHGVLHLCGYSDKKPADKQKMTKAENKYLSLRTF"
     gene            complement(43964..44491)
                     /locus_tag="PKX59_02275"
     CDS             complement(43964..44491)
                     /locus_tag="PKX59_02275"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HOZ89723.1"
                     /translation="MAVSKPFNLQQWVEENRHLLKPPVGNKQVYIDNKDFIVMIVGGP
                     NSRKDYHFEDAEELFYQIEGDIVVKTIQDGKPVDIPIKQGEMFLLPPRVPHSPQRGAN
                     TIGLVVERYRKPDEMDACMWFCEKCNHKLYEEFFPLKDIVNQLPVVMNDFYNSLEKRT
                     CKQCSTVMEPPVPAK"
     gene            complement(44509..44796)
                     /locus_tag="PKX59_02280"
     CDS             complement(44509..44796)
                     /locus_tag="PKX59_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89724.1"
                     /translation="MSNPIVDQVSKAANINQEQAAKAIEAVSAALKEKLPYLLHNQID
                     ILLTGGNLSDGVKQKFETLKDDLESSAKDLSTKAQEFTEEVSKKLNNLFQK"
     gene            44871..45659
                     /locus_tag="PKX59_02285"
     CDS             44871..45659
                     /locus_tag="PKX59_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ89725.1"
                     /translation="MNLDLTNKKALVCGSTQGIGKAAAKELCLLGAKVVLLARNEQKL
                     KAVAAELNTFCKTTPEYIVADFSDPENLRQQVRAYINKNGGFNILINNAGGPPAGNIT
                     DAAAESFIQAFKEHLICNHYLALECIAGMKEMGYGRIINVISTSVKQPIKGLGVSNTI
                     RSAVGNWSKTMSIELAPFGITVNNVLPGATLTARLEQIIADKVQKTGKTEKEVIESLT
                     SEIPAKRFANPEETAAAIAFLASPAAGYINGTNVVVDGGRTACL"
     gene            complement(45662..47746)
                     /locus_tag="PKX59_02290"
     CDS             complement(45662..47746)
                     /locus_tag="PKX59_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ89726.1"
                     /translation="MPLFASGRVIGAIEIASLNKLTASGLNYLQRLIEPVSASIYAVY
                     SNALTRELLEESIMQSEELVAQKQELAQVNHNLTIKSEELEVSQKELEKQQEELKAAN
                     ANLEIKAHLLEERSLAVEEARQSLAFKATQLEQTNKFKSAFLANMSHELRTPLNSILI
                     LAKLLAGNKNKNLTDKQIEHASVIHKSGSDLLMLINDILDISKIESGKLEFVFEKMNI
                     ETVANDINLLFKEFANEKQINFQVKVWENCYPEMVSDKLRLEQVIKNLISNALKFTDK
                     NGQVSLDFRKATTEDCKTHNLHFEEDIIAISVTDSGIGIPVEKQKQVFEAFKQADNST
                     SRKYGGTGLGLTISREIVQILGGEIVLESTPGKGSTFTVFIPFFASCESTHDTPVKKV
                     ITQAETEKQENIKHESPLISTIVSKFPDDDFTDDRNFITPFDKVILILEKDITFLKTL
                     IDYCHIFNYKAIASQNGETGILLAKNYQPHVIIVDHNLPDIDGGTLLKNIRSEQKLSH
                     IPIHIISELKPTEGLINEFNITAYHHKPLNKKQLENVMNELQAPVNITKKETVVEHTF
                     HESSLSGKTILMADDDLRNIYALTTLIENAGANLICAYDGKEAINKLEKNPHVDIVLM
                     DVLMPVMNGVDAIAEIRKTAQFRNIPVLAVLSGSDAQEKEKCLDAGATDTLSRPLNND
                     QILNKIKFLLYQ"
     gene            complement(47815..49014)
                     /locus_tag="PKX59_02295"
     CDS             complement(47815..49014)
                     /locus_tag="PKX59_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89727.1"
                     /translation="MKSNKWSFKIDKKLYVIFIMLIVITLANAFISTYIIDKSKNITI
                     EIAEVTTPSQSAISDLTQLVNNSWLQATNVAFRKNKTASISELQFINETQYPALKKEL
                     KRLSESWQQKEQLALIGLFEKYDRLIYFELQLIEKARVSTENNVEILETQITPQVQEI
                     TALLSDFNIKKRAETTAKQKEMIQSFIVLMAVVLGLAMVIINFIIVITLFLNKQVVNP
                     LMKIRRYLLQLGKGELPELSIKTPKNAVGEMIDALKTHVSGLNRTAEFANNIGRGNFD
                     YHFEPLSENDVQGKALLDMRSKLKAASDEDASQKWISEGLERISVLNKDYTLDMQVLC
                     NNLIKEVVEYSGACHGTVFLLDTKDESLHEVDLKGIYCRSQQLTDLHIEWIKHQLVKN
                     VLTITKE"
     gene            complement(49117..49187)
                     /locus_tag="PKX59_02300"
     tRNA            complement(49117..49187)
                     /locus_tag="PKX59_02300"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49153..49155),aa:Cys,seq:gca)
     gene            complement(49290..49640)
                     /gene="folB"
                     /locus_tag="PKX59_02305"
     CDS             complement(49290..49640)
                     /gene="folB"
                     /locus_tag="PKX59_02305"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOZ89728.1"
                     /translation="MHQLILSGIECYAHHGCLSQETVIGGRYRVDITFYADLSIAANS
                     DNLTQTIDYVEVNDMVKIQMAIPSKLIEHVGSRIAQKMKERFTICNKIKVAITKYNPP
                     VQGFIREATFVIEI"
     gene            49725..51260
                     /locus_tag="PKX59_02310"
     CDS             49725..51260
                     /locus_tag="PKX59_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOZ89729.1"
                     /translation="MKIRLVLILTFINFSLLMAQQPITLNVPQPDQDWPNSVTYHIFV
                     RSFCDGNGDGIGDLKGITARWEYLRKLGAEAVLISPIFASPSYHKYDVTDYYKLSPEY
                     GNIESFQYFIKDAHAEGFKVILDIPLNHCSNKHPWFIEAAKNPASTMRNYFVWSNNPP
                     ATEKDKWHLPKDEKGNTIGKTYYYGFFSPEMPDWNYDNAQVVEEAQRILKFWIDLGVD
                     GFRLDAAQHIFKDHNKNIGFWNMISAYVKSINKHTFLVGEVDNSSDMVAPYLKPLDAC
                     FDFDLSREVVQSLKNENASNFPMRMEKIITHYKAVNKDYKDAIFLTNHDQNRVMSEVG
                     NNVDKAKLAANILFTLPGIPYIYYGEEIGMKGEKPDEFIREPMLFAPEKEDEMRPKWM
                     KPKYSTDKTVESAMVQIKEDQSLWNHYSRLISMRKENRALQYGQFENSSLSSKSVLAY
                     YRIYNQMRVLIINNVSATAVTLTKEEKDNTLINPIYQTSKQTTVKGNKITLAPYSTII
                     LTN"
     gene            complement(51367..52905)
                     /gene="gltX"
                     /locus_tag="PKX59_02315"
     CDS             complement(51367..52905)
                     /gene="gltX"
                     /locus_tag="PKX59_02315"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOZ89730.1"
                     /translation="MQRRIRTRFAPSPTGPLHMGGVRTALYAWLFAKKNGGDFLLRIE
                     DTDQNRFVPGAEQYIIDALHWCGITIDEGVSVGGTHAPYRQSERKEVYRSYAMQLVAS
                     GNAYYAFDTAEQLEQQRKNAEEKKETFSYGSATRMSLNNSLALTVEEVKELISKNVPY
                     VIRLKVQENETITFTDLIRGEVSFDSNLVDDKVLLKSDGMPTYHLAHIVDDYLMEITH
                     AIRGEEWLPSAPAHILIYRYLGLTAQMPHYAHLPLLLKPDGKGKLSKRDGDRLGFPVF
                     PLEWKDPTTGEVSSGYRENGYYPEAFVNMLAMLGWNPGTEQEIFNMNELIAAFSIDHV
                     HKAGARFDPEKTKWYNTQWMLRQSDEDIAKRIKPMVASLTGITTDDVRLSDSFLTACV
                     ALLKPRVQFEKEMPEKGLYLFIEPTEYDQTVISKKWKPEFKDFFKMLSDEFTASNEWN
                     SQIAEQTFKAAAEKNNLKPGEVLQLFRVFVSGQAQGVDLFPMIQLLGKNQVLKRLESA
                     LKLV"
     gene            complement(52951..55494)
                     /locus_tag="PKX59_02320"
     CDS             complement(52951..55494)
                     /locus_tag="PKX59_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOZ89731.1"
                     /translation="MNFTKALFAAIISIALLSSCKTTQKTPVETHDLPAIEVKPPYED
                     YRASNTILNDLQHTKLKVRFDWNKKYLYGIAEVTAKPYFTSVNTLILNARGMDFNEVS
                     LITDTGRKKLTYSYQNDSLTINLDRFYNRTETYTVYIDYISKPDELKNIGGSVAINSD
                     KGLYFVNADGKNPNKPRQVWTQGETQSNSVWFPTIDSPNQNMTQEISITVDSSFVTLS
                     NGLMTSSVKNADGTRTDTWKQSLPAAPYLTMMAVSNFKVVKDRWRNIEVNYYVDPEYE
                     MYAKDIFGNVPEILDFYSRKLGVDYPWEKSSHVVVHDYVSGAMENTSAILYGEFLQRT
                     KRELLDGTSESTIAHEIFHHWFGDYVTCESWSNIPLNESFATYGEYLWDEYKYGREEA
                     DKGGMDDLNQYLAEAKYKQVDLIRFHYNAREDMFDRHSYSKGGRVLHMLRKYVGDDAF
                     FASLKKYLNDNKFSPVEAHNLRLAFEAVTGQDLNWFFNQWFFAKGHPIIDISYQWNDT
                     LKKQTVIIEQKQNLQETPLYKLPLAIDIYENGKVRREQVTLTQQKEYLFFDCNSKPQL
                     INVDAEKQLLCAKKDNHTSDEWRYQYQHAPLFLDRYEAIDALAKGYEGNSAEAKIIIS
                     ALDDKNPSIRALALRNIGAAAKADSTVKNKLQQMVTKDAKAHVRESALSTLYKYFDDS
                     SLDNIAINDSSYNVMTTALEHYITKNPKQLSSLKKYESDNNNNLLYTLSGLYAQYGSD
                     ENYDYMINALNVASGFGKMGIIQNLGTFLEHSSPAVTEKGVDALTDAAKNSSSKFMNM
                     QTSNTLSKLKKFVTNKIKELETKPEYFDQLSRYKKIEEKLTSSITSLKK"
     gene            complement(55851..56498)
                     /locus_tag="PKX59_02325"
     CDS             complement(55851..56498)
                     /locus_tag="PKX59_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOZ89732.1"
                     /translation="MELYKELSETQIAERILNGEKSLYEIIVRRFNPTLYKIGRSYNY
                     NHEDTEDLMQDTFVDAYRNLSQFEGRSNFKTWIIRIMLNNCFRKKEKSGFKNEIKQDV
                     HENSQPMFTGTNNDTQKLIQNRELGDIIEEALSKIPDEYRMVFSLREINGMNVSETAG
                     LLNISEANVKTRLNRSKAMLRAEIEKSYAAEELFEFNAVYCNAMTDRVMKIIDKL"
     gene            complement(56503..56874)
                     /locus_tag="PKX59_02330"
     CDS             complement(56503..56874)
                     /locus_tag="PKX59_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89733.1"
                     /translation="MENKIISQTFNLLKYTFVVVPIVAGADKFTNLLTNWPQYINPTI
                     VGMLPLTPAAFMMLVGVIEIVAGIVVLKKPIIGGQIVAAWLTLIALTLLMGFNYVDVA
                     VRDLVMAISAFSMAQLAKFVK"
     gene            complement(56899..57606)
                     /locus_tag="PKX59_02335"
     CDS             complement(56899..57606)
                     /locus_tag="PKX59_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89734.1"
                     /translation="MKNTSLHHINSLEPAETSSITKRKLNLKSLSVFLLLFSLATLIT
                     SCKKDDDSNPLESTQYYNLQDGLRKLWNDHMKYTYATVDAYYNNPSGLSSSLNRLLQN
                     QKDIGAAIVPYYGQAAGDTLSSLLTTHINLAVPVLEAAKNNDQPALNLALANWKTNAN
                     DIANFLSTANPKNWPPHDMQHMMEMHIDQTTTYAVDLLNKDYNNAITHYDEANQHMLD
                     MADDLAKGIALQFPEKF"
     gene            complement(58474..58860)
                     /locus_tag="PKX59_02340"
     CDS             complement(58474..58860)
                     /locus_tag="PKX59_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOZ89735.1"
                     /translation="MNTSIENELSYQIIGIAVEMHRVLGPGLLESAYESALTYDLIQA
                     GFDVKVQVPMPLIYKNVKQEVGYRLDLIINDKVIVEIKSVETLAPVHFAQTLTYLKLS
                     GKKLALLINFNTKYLKEGIHRIVNNL"
     gene            complement(59270..59911)
                     /locus_tag="PKX59_02345"
     CDS             complement(59270..59911)
                     /locus_tag="PKX59_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89736.1"
                     /translation="MTFHKSVFLLIFLFATNFCSGQIDIYANKYPLFGTTRCGICFGN
                     WCNYNGYRFNNTDTASSPFNGINIFMYSDGNSGNGVQIGVIRAEYYQFNGVSFASISQ
                     YGSKLNGVGISAFGVVPDTLNGLGVGLFLSSDRKHEERILNGVGLSIFGADAGKMNGF
                     YIGGYCSSDRQKGLSIALFNHTKELHGLQLGVINYAGNNRKLFRWMPFFNVHL"
     gene            complement(59946..61382)
                     /locus_tag="PKX59_02350"
     CDS             complement(59946..61382)
                     /locus_tag="PKX59_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOZ89737.1"
                     /translation="MSQEAIIDQLNKLNLISKQLDLPESQRTELLHQTADYANNFIST
                     LHTIKGYQEKTVGDLSINNKPAALHDILNLYQKEVAESGINAASPKHLGYIPGGGVYT
                     AALADFIAAVTNPFAGAYFAAPGAATIENEVVNWLKNVFSFPERAVGCLSSGGSVSTL
                     IAFTAARDKFKIKNEVVPKSVVYLSEQVHHSTQKALRIIGLEDVIVRYIPLDDCHKIK
                     PDALIALIEKDKAEGLHPFLIVATAGTTDTGTVDPLNDIADIAAQQKLWFHVDAAYGG
                     FFILTSKKNLFKGIERADSIIVDPHKGLFLPYGVGAVLVKDGNAVLHSHYYTANYMQD
                     GQNEELLKSPANLSPELTKHFRGLRVWLPLKYHGIEPFIACLEEKLLLVQYFRMKLAE
                     QGFCLGPEPDLSVSYFWYPFATDADEKNKLLMEEMHKDGAVFLSSSIISNRFVIRMAI
                     LAFRTKKETVDEAVEMIDRCLKKVMLNS"
     gene            complement(61444..61881)
                     /locus_tag="PKX59_02355"
     CDS             complement(61444..61881)
                     /locus_tag="PKX59_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOZ89738.1"
                     /translation="MKERFNLSKADPKAFDAMLNLEKYLAESGLDKILFELIKTRASQ
                     INGCAYCINMHVRDAMKIGETSQRLFLLDAWRETTLFTEKERAVLALTEAMTLIANNL
                     VSDTVFDDAKKHLTDKEMAAVMMTIVTINGWNRIAITSRTALD"
BASE COUNT        19271 a        11095 c        11005 g        20679 t
ORIGIN      
        1 atattacata ggttaactgt ttattatttc tgaaaacatc ataggttaac atcttctgtt
       61 atcttacttt tgcactatgc agtttaaaga agttgtagga caaaaagaca ccaagcagtt
      121 gttgttgaaa atggcaggtg agaatcgtat tccacatgcc atgcttttta tgggaaatac
      181 cggaagtggt acactacctt tagcaattgc ttttgctcag ttaattaact gcgaaaatgc
      241 cgccacagac gatagctgcg gaacatgtca cagttgccgc atgtatgcaa aacttacgca
      301 tcccgatttg cattttgcac ttcctgtaaa tactattgat gatattaaaa aacctaaaac
      361 agatgatttt attgagcagt ggcgtactgc atgtctcaaa aacccatacc tttctctgca
      421 agattggtat gataccatag gtattgaaaa aaagcaagga ataatagcta ctgaggaaag
      481 tactgctatt gtacataaac tttatttaaa aagttttgaa ggcaaacaca aagtgctcat
      541 catgtggcat gccgacagaa tgaacagtgc aacaagtaat aagcttctta aaataattga
      601 agagccaccg ggaaacactg tgtttattct tatcaccgaa cgttatgatg gtatattacc
      661 aaccatactt tcgcgtacac aattgataaa aattccgatt atagataatg attcgctgat
      721 aaagtatgcc tgcgaaaatt ataaccttga tataaacaaa gcccgcaata tggttcatct
      781 ttcgggtaat gatttaacag agttgcaggc tttgatgagt caggaaaatt tgtccggaca
      841 aatggaactt tcttttattg agtggatgcg tatgtgctat aaatttggaa taaattataa
      901 aggactatac agttggattg aagacatatc aaaactatcg cgcgatgcac aaaagaactt
      961 tttaaatttt agtctgggca ctgtccgcga atgtttaatt accaacatgt caggtataaa
     1021 tcattcaaga tatacagatg caacgtttaa tggtttcgaa aaattcagca agatgataaa
     1081 tgctgaaaac attgagcctt ttgcaaaaac acttgaagaa gctatttatc atattgagcg
     1141 caatgccaat ccaaaaatat tatttttaga tttgtcgttc agaatgcatc gcattttgca
     1201 catgaaattt tgattgatta atgaataccg aaacaataaa tacaaataca aaaagtacag
     1261 gttgcacttc atgcggaaca ggcagttgca ataaactgaa tgtttacgac tggcttagtg
     1321 atatggagtt tccaccggga catattccat tcaacgtagt agaagtgcag tttaaaggaa
     1381 cccgaaaaga atatttcaga aatacagacg gacacatact taaagccgga gatatagttg
     1441 ccgttgaagg aaatcccgga catgatattg gcaccgtcac catgacaggt gagttggtta
     1501 aacttcaact taaaaaacgt ggtttcgaaa gtggtgctcc ggagattaag caactgtaca
     1561 gactggctaa acaaggcgat attgaaaagt gggagcaggt aaaacaatta gaacatcaaa
     1621 ccatgcaccg tgcacgtaag attgcattgc agctgggtct gaagatgaaa cttagtgatg
     1681 tagaatatca gggtgatggt aagaaggcta ccttttttta tactgcagaa gaacgtgttg
     1741 attttcgtga gttgattaaa aaactacatg aagaatttaa ggtgagaatt gatatgaggc
     1801 agattggtct cagacaagaa gcaggacgtc ttggtggaat aggttcgtgt ggaagagagc
     1861 tttgctgttc tacatggatg acaagtttta aaactgttgg tacatcagcg gcacgatatc
     1921 agaatctatc catcaatcct gtaaaacttg ccggacagtg tggtaaacta aaatgctgtc
     1981 tcaactttga gttggacagt tatatggatg cccttaaaga tattcctgaa caaggtactg
     2041 ctatttttac tgaagcagga aaagcattac acagaaagac agatattttt aaacgcatta
     2101 tgtggtatga actaattcct aatttaaaag atggcgaata ctaccaaccc agtgcagata
     2161 aatgggtaat gattcctgtt gacagagtaa aagaaattat tgcactcaat aaagaaaacc
     2221 aaaagccggc tgatctgaag agtggtcttc ctgtagaaga agaggtagaa gagccggatt
     2281 atgccgacat ggttggacaa gatagtctta ccagaatgga taagaagaga aagaagaaaa
     2341 agaagaagaa aaaaccttca cagcctaatc aacaaactaa caatggccaa cagaaaaagc
     2401 cttcttagct atttaattgc aagcgtttta atcttggtat ctgcttcctg taataagcaa
     2461 tatcattatg aagaaaacaa atccataaaa ggaagcaatt gggatttttc tgaaccaata
     2521 agttttgatg tcaacattga tgataccgtc actgcttata acatgtacat taatgtgcgt
     2581 aatcagggta actatcgttt cagtaatttg tttttgtttg ttaacacaca tttaccacaa
     2641 ggacagcatt tacgcgatac agttgaaata actttagcta ctcctgacgg ggcctggctt
     2701 ggcaaaggca ttggtgattt atattccaat cgttatttgt ttcgcaaaaa tttcagattc
     2761 cctcaaaaag gtaactatca ttttgaactg atacaggcaa tgcgtgtaaa tccacttccg
     2821 ggaattatgg atgctggtat cagaatagaa aatacaaaac cataattttt agcgaatttt
     2881 ctttaccgat agaaaaaata aaaagccgcc tcattaactg aaacggctgc ttggttttag
     2941 acttaattat tttattctac aatattctga cgtgcttttt ccaaacctga tgccaatcgg
     3001 gttaagaacg cattgtctat ttgtttttcg tctgcaggtt ctttataaat agctagtaag
     3061 gcttcatagt ctgattttat tttctttgaa tcatcactat cggcaaactc acttaacaat
     3121 ttcgtaatgt tgtcaagata caaacgttgc tcccatactc ttttatacaa tatttcattg
     3181 tcggcatttc tggttgctgt aagcaatgat tttacggtaa gatattgaca ttcgagccag
     3241 cttccggcaa gtacctgact ggcagtctcc aaccttttgt tgctgcgtaa ataagcatca
     3301 gttgcagagt atgcatcatc aataacacga gtcagagaat cacgattgtt gctgtttgtt
     3361 tcaaaacgat ttacaaactg attaaatgtt tcctccatgc ctaattgtgc agcaagattc
     3421 tttgctgtgt tgtaatactt aatggcatca agggttctgt tgttaacaac caaataaccc
     3481 aaatcaacac cataaatccc gtagttcatg gcttgtttaa gagatgtcat gtatttgtcg
     3541 gcattatctg ctgaattgag taatgcagtg tttacttcaa gttttgattt actaaactca
     3601 tctaatacct gcataggtgg aggcaggttg gcaatggtgt aggtaaactg aaaagttgct
     3661 acctcactaa ccggcatggt atcttttgtt tctgtagtct gctctttgtt ttcttcacca
     3721 gacttattac tacatgaacc ggtgagcaac aatccggcta tgacagaggt taaatagata
     3781 tatcgtttgt gcatagtttt attttcagca aacgtaacag atttttttac ttaatgcaaa
     3841 aaatgatgaa tcatacttta atttaactgt taatgataga gctaaaacac atgaagaaat
     3901 taataccgct cttgactccc gggtatttgg gctgccttta acgcctaata cataaaatgg
     3961 ttacaacaat cggcatatta atttacaata cagaatgtaa tgtaacctaa tagccttttt
     4021 ccttgtatag aggttataaa attcagtaaa gatgacaaca aaatcatttt ggtcacaacg
     4081 tgccatgctt agtatcaggt caaacaaacg attgtttctt ggctgccttt tggcaggtag
     4141 tgttggcgct tcagcctatt tgtttttaat gcctaaggtt tatgaatcgc aggctatctt
     4201 aaaaaatatt aaccccgata aacagtcaac tgccattgct gcagaaatga ttcgttcacc
     4261 caaaacaatt caacagtctg taaaaggaat ggggctgaac gtagaatatt ttgtaactaa
     4321 aaactttcag acatcagaat gttttgacaa taagcctgta aatatttcat ataaattaaa
     4381 agatgagtcg ttcagtcagc aaagttttag tctgcaacgc gatggtgaaa aaacctacat
     4441 acttgaatat gatgatcagg cagggcatca acaaaaacga ggcagctata atgaattgct
     4501 cgaccttgga tcgcttgctg taaccattga aaaaacgcct tatctgaatt tgcttgatga
     4561 taaagatgaa gtttcattta ccatttattc tgacagccgc ttagctaaca aaattgctga
     4621 gcaaaatctg agttgttcta ttgaagatgg tggtgttata aaactaaaca gccaaagcgc
     4681 taaccctgct aaggcatatc gtatagcaag tgctgtctct aaaggttatg gcaatgccgg
     4741 acaacaggca aacgaatcac attccttagc caatgtgcag ctaatcaata atcaacttga
     4801 ccgtgtttcg caggagttgg ataatgcaca acgcgagttg tcacactttc aaagtgtaaa
     4861 caatgcctat gaaatgccac agcaggcggg agcagtatta aacactgtta gtcaactaca
     4921 gattcagaag gtagaagcag atatgcaatt agctgcctta gataatctga gtgattatat
     4981 gcgcaagaac agaaatgtga acaccatttc accggagtat ggaaccatta cagatattat
     5041 ttatacagaa acatatctga agctgaatga taaagttgcc gagcgtcagc aactgttaga
     5101 gcaaggtgca gacctttcaa aagttgataa ggaaataagc tcgcttaaag aaatgcttgc
     5161 ggagagtatt cgcaatacaa gaaaaaaact gaccatcaga caagaaaccc ttgcagcagc
     5221 tattgtttct gctaaagaca gaattcaaaa tttacctcag acagaagaaa aactacagca
     5281 actaaacaga agcatttatt tatatactaa gctttatgat tttctgattc agaaaagagc
     5341 tgaggctatg gtagaggctc ctgtattgcc accatcatca tacattttaa atgaggcaaa
     5401 aatgcctaca tcacctatca gtccaaatgc agcaaaagtt tggtcgctgg cattgcttgc
     5461 atcactattg tttggtgctt tagttactgc tgttaagccg ctattgaaaa taaaaatcag
     5521 caatagaaaa gacctgcaaa aatttacaga cattcctttt atcgccaata ttgaagccgg
     5581 tggtaaaaat catgatttca gtgagtcgtt tatgacactc tgtactaaaa tactcatgct
     5641 tggaaataaa cagcctgtgc agatgataac tataacatca gcaaaagccg gtgaaggaaa
     5701 aactactgtt gccgaaaatt tagctaaggc tatgggcaag ctgggtaaaa aagcattgtt
     5761 aattgatatg aatcctataa atccttcatt aaaaaacaca cttggtaatg aggaagaaaa
     5821 cagttttgct caggttattg acgatgataa aaatctacat caggcagtac aaataaccaa
     5881 ttatgaaaat gttgatctgc taagtgccgg atatcttgcc catggcgtaa acacacttct
     5941 tataaacgat aaaactggca acattcttgc agatatgaaa acgttatacg attacgtgat
     6001 atttgatacc cccgaaacca gtaattatat ggatgctgtt ccgttgatga aaatgagtga
     6061 tttaaacatg tttgttatta aagcaaacag tgccacattc gatcagcttg agtatgcatc
     6121 gcaaatgaaa aaagattttc aggttgagaa cctatttatg attattaatg gcatgacgga
     6181 aaccatgaac cactccggca tgcaaacttc gggtaaggta aaacaaattc gcagacgtga
     6241 acaagcagag gcagaaatca ggtttatgcc aagaatgatg aaaaaagcag cattatggtt
     6301 ttattgattt aatttttatg ggggataaag caaaattctt aaatcacttt tttgagcgta
     6361 tcagcgacat cagatatgcc gcaatgggat taactgtttc gcatcccgaa gagattgctg
     6421 aagatattat tcagattgca attaaccggg aagatacacg caaaattctt acggtgcttg
     6481 aattttcgcc ttatttaaaa agagtgcgtt tagaagaaaa acccttttac aatttcattc
     6541 atctgaaatt gagtaatggc caatggatac aaattcaact aatctatggc tttgtaagaa
     6601 aaggcattca gtttatgaat gaaaaagagg tgttgaacga gtgtaccatt aacagtggag
     6661 gaataaaagt taccagccag catctgaatt ttgaatatgt gtggctgata cattgtctga
     6721 acaaaacaaa tgttcccaat catcagcatt tatttttctc gcaatataac cgaaatgaac
     6781 gctctgccat ttttgcacac atacgaagca gatatttttt agaactcaat gtattggacg
     6841 aactatatac ttttcacaaa aaaacctatc gtcaggttta tgaaaaaata attcgcagaa
     6901 aagaaaatac atgggcaaaa agaaacattc gtaaaacact ttatgccttt tacagtgcat
     6961 taaaccttat aaagacaaaa agtattaaag tgcagtttaa gggttcggga tatcatccgt
     7021 tttcaaaaac agaaagagca agagtttatt agtcttttct gttttgttta acccattggt
     7081 taatgtactc aacaatttca ttggtgtttg tacccggagc aaacagtttg cccacaccca
     7141 tatcatttag ttttttcata tcggcatcag gaataatgcc tccccctgta acaagtacat
     7201 cggtcattcc tttttctttg agcaactcca taattttggg aaatacagtc aggtgtgcgc
     7261 cagagagaat tgaaacacca ataacatcaa catcttcttg caaagctgtg ttaacaacca
     7321 tttgtggtgt ttgacgaagg ccggtgtaaa taacttccat gcctgcatca cgaagaaaag
     7381 aagctataac ttttgcacca cgatcgtgac catcgaggcc tactttggaa attaatactc
     7441 tgattggtct gttcataagt gcaaaaataa caaaggtgtg ttatctttaa agaacattta
     7501 tcagtattct tgaatgtgat atgttcataa attgatatta tcgtggattt ctaaataaac
     7561 agtaatttta ttttagcatc catgaaaaaa ttagttgttc aattattttt acttttctcc
     7621 tacggaacta cagttgcaca ggatacgaac aataatattc caaaaccgca aagtattatt
     7681 ccgcagtccg gcatctttat tttgcatcgt aaaatagcaa tagtagaagg tcctgcatgg
     7741 gtaacgggtg atgcagcagc gagggtttat tgcacacaat taaaagatag tataggttta
     7801 caccttgcca ttgtgaatgc tatgcgcgaa ggaacatttg aaaatgcaat taatctgaaa
     7861 aaaataaatg atgcagcatt aggaaatgcc ggttacagaa ttaaaattaa aagacctgcc
     7921 attattgtag aggctaataa tgatacggga ctgacgaatg cacttcaata tttatttcaa
     7981 ataataccta agaaattctt tgaggacaaa aatccaaaag caaaaattcc actcagctgt
     8041 agtgagatta catgtaagtt ttgaaagtac gaaggttaga aagttaaaaa ttttgataaa
     8101 tatttaaagc accaagaata agcatctaac tctattttgt aatgacaaaa tattgaatta
     8161 accgatattt ttagattgtt gtttaagtaa gaaggagatt tttttaatac ataacatgaa
     8221 aatatttatt gtatattaaa aaacactatt taaattaatg taactgaagt tgcattacag
     8281 ttatgatttt tatcagacct ttgccgtcac aattaaaatt ctaaaataag gtggtggaaa
     8341 tttttggata tgcatgtgca atacttatgg gattgtcgtt gggattaatt ggtggtggag
     8401 gcagcatttt aacagtgcct atattggttt atttattttc tatagatgtg gttacagcaa
     8461 cagcctactc gctttttatt gttggcttta caagtttgat aggatcgttt aatcatatca
     8521 gaatgggaaa tgttcatctg cgaaccgcag tgctatttgg tattccatca attattgctg
     8581 tattcattac aagagcattt attgtacctg caatacctca gcatttattc aacattggaa
     8641 cactacagat aacaaaaccc attgcactac tgttgttgtt tgctgttatc atgttgctgg
     8701 catcattctc tatgattcgc aagaaaaaag aaaaacccga ttatgataaa gtgcaataca
     8761 attatccatt aattataatt gaagggtttg ttgttggcac aattacaggg ttagtaggtg
     8821 caggtggagg atttttaata gtgcctgcat tagtattgtt ggcgcatttg cccatgaaaa
     8881 aagcaattgg atcatcgtta ttgattattg caaccaactc tttattgggt tttacggtta
     8941 gccttttaca tagtgagaaa acagactgga aatttctgtt aactttttct gctattgcta
     9001 tcataggaat tattaccggc agtatgtttt cgaaaaatgt ttctaacgaa aaattaaaac
     9061 ctgcatttgg atggtttgta ttaataatgg gtgtttatat tattcttaaa gaaactttat
     9121 tgaaataatt aaaccctttc atttcaattt gtgttttcat ttcatttctc tttacaataa
     9181 actttatttt aaatattatt acttaacact aaatttattt taaactatgc ttgaattaat
     9241 tcttaaacca tggccttggt atgttgcagg ccctctgata ggactcattg tgcctgcatt
     9301 acttatactt ggaaacaaat catttggaat cagttcttca ttccgtcatg tatgtgctgc
     9361 ctgtatgcca tcaaacattc cattctttaa atatgattgg aaaaaagaaa ggtggaattt
     9421 attttttgtt gccggaataa tggctggtgg attcttagct gtgaatttac tctctgcatc
     9481 tactaaagtt gatatcaatc cttcattagc agcatctcta aatgaaaaag gtataaccga
     9541 ttttagtggt ttggttccgg cagatttatt taactggaat agtctgttca cattaagagg
     9601 ttttattatg atggttgtag gaggattttt agttggcttt ggaacccgat atgcgggagg
     9661 ctgtaccagc ggacatgcta ttatgggatt atcaacgctg caattgccct cgctcattgc
     9721 cacctgttgt tttatggccg gtggttttat aatggctaat ttaattttac cttatatact
     9781 ttcactctga aattaatatt atgaaaacag aagttacttt agaaaaagaa caaaacacag
     9841 attttgaaac acgttcatta gatacaattt gtgtaaacga aagtcatctt gaacatagtt
     9901 gggagcataa cttaaaatac agccttatgg gaattctgtt tggaattatt ttagtgaagg
     9961 cagaagtggt atcgtggttc agaatacagg aaatgttcag gcttcaatca ttccacatgt
    10021 atggtatcat tggaagtgct gttgttgttg gagctttgtc tgtttggctt attcgtaaat
    10081 tcaacattaa aacaatttat ggagaagaaa taaccctaca tccaaaagaa tttaataaag
    10141 ggcaaatttg gggtgggcta cttttcggat tgggatgggc tattacaggt gcatgccccg
    10201 gaccactgtt tgctacaatt ggctctggtg ctactgtaat tgttgtgagt ttattaagtg
    10261 ccgttgccgg aacatgggtg tatggtaaat tccgagatag acttcctcat tgattaaaag
    10321 tttgttgaat ccaattgtat tatttaattt agtaggataa aattattctt ataaatttat
    10381 tattgataaa cttttaatct ttataaatat gaaaaaatta ttttacattt tattattcat
    10441 gactgttgga ataacaactg gattttcaca aaatctgaga aaaactaata atgcagtttt
    10501 aaggtttgac tcatcgcatg ataaaccaat acaaaacttg gccacagaat ttatttcgaa
    10561 caatacttct aaaatacaat caactgctcc ggcattttca tttactgaaa gtccatataa
    10621 tcataatatg catattacct ctgatggtac atattattat accataaatg gtggtagtgc
    10681 ttcaaatggt cagataaata aatttgattt aaacggtgtt ttacttcaaa ccttttcaat
    10741 tggcattgat ggcagaggtc tgtcatataa ttttactgat ggctttttat atgcatcggt
    10801 ttatggagga gatatagtta aaattacaga tttagttacc ggagcattta caactttgtt
    10861 ttcaggtgca atgcaaaata gtcaagcctc atttgctata tctcctgatg gaacaaaatt
    10921 ttatgacttc tttcagggaa cattacgtat tcatgatttc actacaggtt ctgtaataaa
    10981 caccttgtca ggtcttttat atggtttagg aaattatggt ggcgaagctg cggttgctgt
    11041 tgattcaagt catatttata cttgggatgc tgtaacaaga aatgtatatt cctataatct
    11101 caccggaaca cttatacaaa cattaccgct tgattcaggt gacaatggaa tatctttaag
    11161 tattgcaaat ggatatttat ttgtttcaaa agatgctaat tatgccattg gcacctggta
    11221 tggatatagc atttcatcat ctacatcaat aaccgaactt caaaataatg aacctataat
    11281 tgtatatcca aatccaacat caggagtcat aaatattgat gcggacataa aaactattga
    11341 aatatttaat gtttctgggc aaaaaatatt tgccactaca aatattaata atttatatca
    11401 aaacaaactc gatttatctc atttatccaa aggaatatat tttgcaaaac ttgatggtgg
    11461 cgattttctt caaacaacaa aaattatcat tcagtaattc tatttctcta atatgttttt
    11521 actttctata aaaagtttag tcctgatttt agatttattt tagttgttga aacaaatcta
    11581 tttttggtct gttaaacata ggttgtttaa acatttattt atgtaataac tgtagtttat
    11641 ttccttaata tgataagtgt ttcagaaatc attatacggc tttcattagc atcgtttttt
    11701 ggtgcgctta taggattaga acgagagcgg aagcattggg ctgccggttt gcgtacacac
    11761 atgcttgttt gtgtaggttc ctgcctgatg atgatggtat ctgcatttgg attttcagac
    11821 atcttaggaa ctcctgatat aacacttgac ccttcacgta ttgcagcaca agtagtcacc
    11881 ggtataggtt ttataggtgc gggtgcaatt ctattttcga aaaatgaaac tattagagga
    11941 ttaactaccg ctgcaggaat atggactgtg gcaggaattg gcttggcaac aggaggaggc
    12001 atgtatctag ccgctgctgt agcaacggct attgctataa ttattttatg gggacttcag
    12061 cctatagaga aaatgtattt tgaaaaatat aaacaaaaaa gtttgcgtat aactacaagt
    12121 catgattttg gtgattcaga atttctgaca gcacttttaa aagaaaatgg catattatta
    12181 atttcattaa attttacaag aacaaacgaa tacttgattt ttcaaattca gctcgaacat
    12241 gctgatgctg gaacgataga atcagttatt aatattctca aaaaaaattc agccattaaa
    12301 gaaattcaat ggcttaacta agaattgaaa actctttcaa ttctaaaaat taagataaaa
    12361 acaaaagttc cgtttccttg tttaggactg tcaatacttc attagaagtg atgatttaag
    12421 tcagctattg atttatgatt taattttaat ttagcatcta aagatttaca aaagttctta
    12481 attatgatta acataggaat aaataaaaaa caggcaaaac aacttgctgc aaaacttaat
    12541 cttctgcttg cagattacat ggtattttat cagacaaccc gcggattgca ttggaatata
    12601 agaggtgaga aattttttga attgcatctg aagtttgaag aactgtacaa taacctgtta
    12661 gttaaggttg atgaaattgc agagcgtatt ttaactttag atgaaacacc gctgcatacc
    12721 tttagcgatt acagtaaagc atcgcaactg aaagaaatca aaaatgtatc agacccttca
    12781 agatcaataa aagcaatttt agatgctttc gaaactattt taatcaaaca gcgcgagatt
    12841 ttaagaatgt cggcagatgc agatgacgaa ggcaccaatg cattgatgag taattatatc
    12901 agcgagcagg aaaaattagt ttggatgtat agtgcatatt acaataaata aaatggaaac
    12961 aacaacacaa aacagaacat ttaatgacat catcaatggc agtaaaccgg tattggttga
    13021 tttttttgcc gactggtgcg gaccttgcaa aatgatggca cccatactgc atgaagtaaa
    13081 aaatgaagta ggcgaaaaag cagtaatact taaaattgat gtggataaaa atccagctgt
    13141 tgcagcagct tatgagatac agggagtacc aacattaatc ttgtttaaag ccggaaaagt
    13201 gttgtggcgg cagagcggtg ttgttaacaa accacaacta ctgcgaatca ttgaacaaaa
    13261 tatagggtag gttttatatg gaatgagcct acaaagctaa ttccataaac acaaggaata
    13321 ttactgctcc ttagggtaat aaactgtaaa aaccctatta aggttaaacc caaaatgaat
    13381 accgccatca agccagtcgc cagtagtttc ggcaataaat cctttttcta ccatactgcg
    13441 tgagttggta aaatgaattt gaaaaacgtg acctccggtt tcaatatcaa agcctacagc
    13501 aagtgagttt ttatatgtgc tgctaatctg atcaggaaat acataataat actctgcagt
    13561 aaatgctata ctcttggaaa tttttattct tccgccaccg cctaatgcaa atacatcatt
    13621 tttttcatta gctgtgggta ctaaattgcg gtgaatgagt gttggcatta gctgaacaga
    13681 aaacgcatta gaaagtttag atgctgccaa caactgatag gaataggtta gcctggaaga
    13741 aaaataattt ttacgttgag ggtctgtaaa ttttaatgag tttagttgca tggatgcaac
    13801 ggcagtaagt gtaaccggtg ttccactttt acgttgcttc agtaatttaa cttttaataa
    13861 tccatcatac attttttcaa atgtgctgcg ccctattcca acgtttaacc agtcggtaac
    13921 accatagtcg aaacctaaac gcatggtagc attatctaaa ccaaaaaact cataacctcc
    13981 actatttatc tgcccgaaac gatgcaagat cattaactgc aatacacctt gcttattatt
    14041 ttcaacactg tgcccgttaa tgagtctggt tgatttaaat gttgccgtta cttcttcttt
    14101 cttttctttc tctccacttt cattgagcat tttcatcagg tcgtcctgag ctgttgcatg
    14161 cagtgtgata aaagtaaatc caataaaaaa cagtttttta atcgttagca taaattcttt
    14221 tttaatcctt cttctgatat ggagcaagtt caaatttaca agttacctta acaacttcgg
    14281 caatattctg aataactaat ttaggaattt caacattgtg gtcttttaag gcaacattaa
    14341 agctaccttc aattgcagga actcctcctt taaaactgta gtttgcctta taatttgccg
    14401 gtttctcaat accatgaata gtaatatttc catttattgt tacctgatag gaagtgtctc
    14461 tatccgtaat caatggctcc tgaattttac ctttatatac agcatctttg tatttatcac
    14521 tttcaagata tttttcattg aagtgttctt ccatcaacgc tttttcgaac ttaaaagaac
    14581 ggatagctac taacgaaaca atatcgcctg ttgtaacatt caaaatgcaa tgcccttttt
    14641 tgcttgtagc atcaatgttt tcgagcggtg cttctgaaaa gaaagaaatt gtacctgcat
    14701 tggttgtaaa cacattttgt gcttgtgtat aatacatgcc tgtcattaat acacataaaa
    14761 taaatttttt cattttttaa tatctaatta ttgggtgctc cctgattaat ccagattgaa
    14821 acttttttaa tgttgcaatc acttaattta ttttgatttt ttggcatagg ttcatatccc
    14881 gaatcatgat tgagtgaccc taatagtttt ccattatcaa cataggtctt aattgctgca
    14941 taagtatcaa taacaacgtt gccctgcgcc atagatgccg aatgacaaac tttacaattg
    15001 ctgcttataa ttggtgccac tgtttgtgaa taggtaatgt taagtgtatc acagttgcct
    15061 gtatttggat ataactcatc ttctacatca taataacaac tactaatgcc gagtgcaata
    15121 aaaatacctg aaataagaat taaactttta ttcatgcttt gttaattgtc ggggtaacca
    15181 tcgttaaccc atttaatgat ttgttttatt ttacagttag gaatatattt tgttgaatct
    15241 tccttatcag gcatggtaaa atatccatct gcatgagtta tagcgccaac caactgtcct
    15301 gtcattgcaa cagcagatac atcatcataa gaaaccaatg aaagacttcc caatggtgca
    15361 attccactat ggcaggtctg acaaatggtt tgcataatgg gtaagatact acctgaaaat
    15421 gttacattaa cactatcaca acccaaacat tcattgtttt tagcaccttg cattatccat
    15481 tggtaaatat catttatctg tacgctgctc aatggtgaac ccggtggagg tggcatgata
    15541 tcatcaggat cattttcaat aagtgatttg tagagtttgc tgtcttcggg tttaccggct
    15601 ttcacttttc ctgttctgat aatgttggca taagtatcta aaataactcc atctgctgca
    15661 gttgcagcat catgacatcc gctataggcg catgaggcat ttattatagg ctgaatgatg
    15721 tttttaaaat aaatggtatc gttgctgcaa tcttcagtgg cagtgctgtc attggttgca
    15781 atggctaagt cctgtggttc gtgcttacag gaagatgttg caattgtcaa tagcatcaac
    15841 caaaaaaata ctgttctatt catgcatgaa ttaatttaga aacaaataaa aaagatatat
    15901 tctaattaaa gtctataaaa atgtattaat gggaaaattt atcggctgaa aggtaataaa
    15961 cacaaatatt cattgtttca acatgggcat ttgagttact tttgctcaaa ttttaagata
    16021 tgaacggcat gcttttgttt ttagataatc ttggtggagg cgaattgttg gtaatcatgg
    16081 tgtttgtatt attcttcttc ggatcaaaga aagttcctga cttagccaga ggcttgggcc
    16141 gtgccagccg cgagtttaaa gatgctatga atggtgtgca gcgcgaaatt gagcagagta
    16201 tgcaaactcc gccaccatca caacaaccac aaaaaaatgt tgaaaatcct gttacccccg
    16261 aaaaaaatgg gggggtagat gaaaaaactg actaattttt ggtgaaacac tctatttttt
    16321 aggggggtgt ttgaaaaaaa acaatatttt tcttgacatt gccctttttt tcaatgtatt
    16381 tttgagccgt tttaaaaaaa atccaatcac tatatgagca atgttcgttt ccgtgccctc
    16441 gatgctacta atgggcgcat acctgtaaaa attacccctc cgtcagtaaa ggtatctgac
    16501 tattttgcca ccaatgtatt caccaaggaa gctatgttta agtttttacc taagaatgca
    16561 tacaatgctg ttatctctgc aatagaaacg ggtgagaaaa tagaccgtaa aatagctggt
    16621 gaaattgccc atggcatgaa agaatgggct atcagcaaag gtgctacaca ttacacgcat
    16681 tggtttcaac cactcactgg tgctactgcc gaaaaacacg acagcttttt cgacatgagt
    16741 aatgataact ctttcgaaca tttttcagga agttctttag tacaacagga gcctgatgca
    16801 agcagttttc caagtggagg aataagaaat acttttgaag cacgtggcta cactgcatgg
    16861 gatccgggct ctccggcatt tattattgaa agcccaaacg gacgcacact ttgtattcct
    16921 acaatttatg tttcttacac aggtgaaaca ctcgactata aagcgccatt gttaaaagca
    16981 ttgcatacac tgaacgaagt agcagttccg gtagcacaat attttgataa agacattaca
    17041 cgtgttagta caacattagg tattgaacaa gaatattttt tagttgatac ttctttgttt
    17101 tatgcacgac ccgatttggc aattacagga agaacagttt tcggtcactc acctgcaaaa
    17161 ggacaacaat tagaagatca ttatttcggt tctattcctg atagagtaaa tgcctttatg
    17221 cacgattttg agattgaagc atataaactt ggtattccat taaagacccg tcataatgaa
    17281 gtagcaccat gtcagtttga atgtgctcct atttttgaag aaataaatct tgctgttgat
    17341 cacaatcaac ttcttatgga tttgatggat aaggttgccc gcagacatca ttttaaagtg
    17401 ttatttcatg aaaaacctta tgcaggaata aacggtagcg gtaaacacaa caactggagt
    17461 atggttacca acaccggaaa aaatttattg tcgccaggag gaaatccaaa atccaacctc
    17521 atgtttttaa ctttttttat caataccata aaagctgttc acgaacatgc agacctgttg
    17581 cgtgcaagca ttgcttcggc agctaacgac catcgtttag gtgctaatga agcaccaccg
    17641 gccatcatga gtgttttcct tggtgcacag ttaagcagca tcttagataa cattgagaaa
    17701 aaagttggct ccagcaaaat gagtgtcgaa gataagacag cattaaaact caacataggt
    17761 aaaatacctg aaattttact tgacaataca gaccgcaacc gcacatctcc ctttgccttt
    17821 acaggaaata aatttgagtt tcgtgcagta ggttcttctg ccaattgcag caattcaatg
    17881 gctattttaa atactattgt tgccgatcaa cttcgcaaat tcagaaaaga agtagatgct
    17941 ttaattaaaa agaatgtaaa gaaagacgaa gctattttgc gtgtgctgcg tcagtatatt
    18001 gttgaaacaa aaagtattcg ttttgaaggc aatggctata gcgaagagtg ggtaaaagaa
    18061 gctaaaaaga gaggactttc caatcatcag acaacacctg ttgcattaga tgcaatggtt
    18121 tcaaaaaaat ctctgaaact ttttgaagac aatcacattt ttacgcatcg tgaagccgaa
    18181 gcgcgacatg aaattatgct tgaaacatat atcaagaaaa tagaaattga aagtcgtgtc
    18241 attggtgacc ttgcacaaaa tcatattatc cctgcagcat tccgatatca gaatttttta
    18301 gcagagactg ttgacaacct caaagactgc gatctgaaat cagaaactta tgctacacag
    18361 ctgcagctga taaaagaaat cagtgcacat gtaaatgcca ttaagaaaaa tgttgatacc
    18421 atgcttgcat cgcgcgataa aggaaataaa atagcagata ttcgcgataa agcaattttt
    18481 tattgcgata aggttaaagt ttcctttgac acaatccgcg aacatgttga tgcattggaa
    18541 atgattattg atgatgaaat atggccaatg gttaaatacc gagaattggt tacgattaga
    18601 taattgattt gaacaacaaa aaaagctgtc tattatggca gctttttttg ttttgaatat
    18661 taatcaaaca cctatctgtt tttcaaaaac ttttaagctt ttaagctttc aaactcatta
    18721 cttcttataa aaattcattt catcaatgta attatacacc gattcaggaa gataaaaacg
    18781 aacgtcattt ccttctttga tggcttgtct gataaatgta gatgatattt caattaatgg
    18841 tgcctgaata atttttacag aaggatgatt ttttaattca ccaccatcag aatccggtcg
    18901 aggataaata tatattttat agttttctaa tatctgctcg aaatttttcc atttatgaaa
    18961 agtgataagg ttatcactac ccattatcag cacaaactca tgttgtggaa atttttcatt
    19021 cagatgaact aatgtattaa ttgtatagga tggtttgggt aatccgaatt ctattttcga
    19081 aatttttatt ttcttatagc tgccaattgc tgtttcaact aagtgtaatc tttggtaatc
    19141 gttaagtaat gatccggcag gtttaagcgg gttgtgtgga gaaacaacca accacacctg
    19201 ttctaaatct gtaaactcag caaaataatt ggcaatcatc atgtgtccgt tatgcacagg
    19261 attaaatgaa ccaaaaaata aaccaacctt catttagtta attcttattt tgtcaattcg
    19321 ggtaattttg tctgcctgat aattgatggt taaaatcaat acacctttat cgtcataaat
    19381 tttccacaaa ccattccgct ggccttgttt atactgtcgc acttcatgca actttccatt
    19441 gtcgttgtaa taaatatgtt cgccatcttc aaggccatca atatattttc ctgtaaatgc
    19501 agttttaccg ttcttatagg tctgatgcca tgtaccaaat tttaatccat cggcatattc
    19561 gccttctgca atatattcgc cattattata tttccatagc ccttcttttt tgccgtcaac
    19621 atatttacct gttgctatta gttgtaatga gtcgttatat tctttagaaa taccttcttc
    19681 tttaccattt acaaaagatt catcacgcct taattcacca ttcttaaaaa accatttcca
    19741 ctcaccttcc ggaagtccgt ttttataatt tccaatttgt tgaactgctc cattctgaaa
    19801 taagaatttc cactcatcaa ttcgtttacc atctttatat tttcctatag aatgcaccga
    19861 gccatcatca tagaaaaatt tccattctcc ctgtctgttt ccattttcat cggttattcc
    19921 ttcagcaatt ggtttatcgt cttcaaataa ttttcctgat gtaacatttc ccttttcatc
    19981 ataaattttt tctacaccct ggcgtttatc gttaattaca tttacaatag actttatttt
    20041 tccacctgca taaaaatcac gctttacttc aaatttagtt gtgctttttt tatcaattat
    20101 caactcgcca tttttatacc tttccgatat aattaatgtg ccatcattat tataaactct
    20161 ataaaaacca ttctttttat cgtcaagata ttctgtttct tcttttaatt tatcgttatc
    20221 aaaaaaaact ttccatacac cttgctttaa tccaaattta tctattctgt taattcgttc
    20281 ttcgctgcgc acataacctt catgataagt tgtgatggta attaatctgc catcctctgc
    20341 ataagactta gcaactccat cttcaacacc atttttaaat ggtataatat ttacaacctg
    20401 accattttct ccaaagtctt ttacccaacc ttgtctgata ccttctttta cttcttcttc
    20461 ttgttttaat tttccatcag aaaaatagta acgctgtagt ccgtttttta atcctttggt
    20521 atatgttaat tcactggtaa tttttccgtt ttcattgtaa aatttccata aaccatcagg
    20581 cttattgtta tgtctgttgc cttcggattt taatttacca ttttcaaaat acgatttcca
    20641 taaaccttcg ggcttgccat ttacgaagtt accttcgcta ctcttattac cgttctgata
    20701 ataaaatgtt ttaaagccat tggcaacggc actgtctgtt tgtgcaaata tgtttgaatt
    20761 aagaaataaa aaaaatgctg caatcagtat tctacaaacc atgttgtttt gatatttcaa
    20821 gcatgcgttt taaaggttgt agtgctgcct gctgcaaatg tttagggata attacttcag
    20881 gtacttcgtt tttcatacag agatacactt tctctaaagt atttaatttc atatacggac
    20941 aatcgttaca tgcacagtta ttttccggtg gtgctggaat aaacattttt tcaggtgact
    21001 gtttttgcat ctgatataaa attcctgact ctgtagctac aataaatgct ttagcaggat
    21061 tatcttttgt atatttcaaa agtcctgttg tgcttccaat aaaatcagca tgacgtaaaa
    21121 catggtcttc acattcagga tgtgcaatta ctaacgcttc gggataacgt tcttttaact
    21181 gaataatttt ttgttctgaa aatatctcat gtactataca actcccatcc cataaaacca
    21241 tatcacgacc aattttttta ttgatgtagg ctccaagatt tttgtcaggt gcaaaaatta
    21301 ttttttgatc aggtgcaaaa ctttctacaa tttttaccac attgcttgat gtgcaaatta
    21361 catcacttaa agctttaatt tctgctgtgc agttgatgta tgaaattaca acatgaccgg
    21421 gatgttgctc tttaaacttt ttaaaatcat caggcggaca actactcgct aatgagcaac
    21481 ctgcatttaa atcgggtaaa acaacttttt tatcggggtt cagtaatttt gctgtctctg
    21541 ccatgaaatg cacaccggca aaaactatca ttgaagcatt agttttttct gcttgctgtg
    21601 ccaagcccaa actatcacca acaaaatcgg ccacatcctg tatgtcggac tcctgatagt
    21661 aatgcgccag aataacggca tttttttctt tttttaattt ttctatttct tcaaacaaat
    21721 ccatggaggc aggaatactt tccggcaaat atccatgttt caaaataatt tcagacactt
    21781 cagtcatttt aaacaattat tattattaaa tatatataat taatttttta aaatattatg
    21841 ttgttataat tagtcggtta atatgttgta taactttttt aaaatgttat tgcattttaa
    21901 gttactgtac atgtactaac agtttataaa ctttttatta taactaactt tatgattaat
    21961 aaaggcataa atataactat taacaacggt taataagcca atatgtttat aaaaaacggt
    22021 taattaaatg tttattatgg tgaatggcta tgtttatata ttgactaaaa cttcgtgcta
    22081 aattattttg aattaagttt aaatctgata aggcatttta gaaataaaca ttgcaaacaa
    22141 aagaaatgat aatctatgaa cagaaaatat aaatatgaac aggaatgttg ttaattttac
    22201 accattataa aatactatta atcagcacta aaatatttga ctgttaatag cagttaataa
    22261 cttaaattta tcaaatgcaa agtataatta taggtagcga tcatgccggt ttcacattaa
    22321 aagaagcaat aaagaaaagg ttttcatcaa cttttcaaat tacagataaa ggcacattca
    22381 gttcagattc tgttgattat cctgactatg cacatgcagt tgccggagag gttggtaacg
    22441 gaaaatatac atttggaatt cttttatgtg ggagtgcaaa tggtgtggct attacagcta
    22501 acagacatag tggtgtaaga gctgcaattt gctggatgcc tgaaattgcc gatttagcca
    22561 gaaagcataa taatgccaat attatttgta ttccggcacg ttttgtcagc gatgaaacag
    22621 cttttttaat gattgaaaag ttttttagta ctgagtttga aggaggtcgg catcagaaaa
    22681 gaatagataa aataaactgc gtttaaaata gaaaaaagat agctatgaaa tttaaattta
    22741 tactgctgat atgtgtactt ccgttttttg tgaacgcaca ggacgaacag gatacatcag
    22801 cagtaacctc taaaaaacaa aaacgtgaaa ttccacgatt tgcaataaaa gtaacaggag
    22861 gtcttagttg gagattagct gatgtaagcc ctgacgagaa tgatttaaca aaagcctata
    22921 ttaaagattt gaaaagcgga aatggctttg aaatttctgc aaaatatttt tttaattcaa
    22981 aattcggagt tggcctaaaa tacaacagtc atctttcaaa ggtcagatat gataacatgt
    23041 atttctccta taacaatcaa aattattttg gcacggtgtc tgatgatatt tcaattaata
    23101 caattgcagt aacttttagc ggaagaattt ataacagaca aaaaacaggt tactttacct
    23161 ttgatggtgg acttggtttg atgacttata aagatgatac gaaaatagct accaataaag
    23221 ttaatgtcaa aggaaacaca ttaggcttta atatttctgc cggtgtgggt gtcaatatta
    23281 caagaaattt atgtttagta ataggtttta attttattaa tggaacctta tcacaatatg
    23341 atgaaacaga aaatggagta aaaaaacgtg ttactttaaa tgaaagtgaa tatcaaagtc
    23401 ttagccatat tgatattttt gaaggaattg tatttacctt ttaaatctat aaatcttttt
    23461 ttctgaattt acgcacagca atcatagttg gtacaataat ccatattaaa agtattagca
    23521 gaatagtaat ggttcctttc tgtgaaccta aaaactcatt aaacaaagca cctgtatagc
    23581 ccataagtgc tgcataatcc gtatgaatga ggatgagtat tctagctaaa tcaactggat
    23641 taaaagtagt cagtatcatg agtggctttt ctattggata gtcaccaaac tgaaacatta
    23701 atagcagcaa taaaccatcg taaagaatgg taaaaaacaa ccagataaaa attgcaacac
    23761 caattccctt agctttatct tttgtaacga tggaaccaag aaaagccaat gaggtaaata
    23821 tcaatgttag caaaataccg gctaacagaa aaatgaaccc tgtggcatcc ggtgtaaaaa
    23881 gcaatatagg caagccaatt cctattaaaa aagccaatgt taaagaaaga acaacacctg
    23941 cataaatact tgtaatgagt ttttttctgg agacaggttg tgctgtaagt aactcaataa
    24001 attcagatga attaaaaata tagatggtag cataaataag gcagaccaaa ggaacaataa
    24061 agagaataat atttaataag ctgattaatc cttttgaagt attttcatca agattaaaaa
    24121 tactgaatcc tgttaccatc aaaaaaacaa gataggcaat tacaaccttg cttttcagta
    24181 tgtcgagcat gatatattta actatcttat tcattattgt tttttaaaat ggttgtaata
    24241 actttagaaa cagatttctc ccctgtttga agttgaagct cttctaattt tttatgaaac
    24301 atcaattccc cttccctgaa aaagataatt tccgaaacca actcatcaag ttcactaaga
    24361 atgtgtgatg taataattat cagtttgcca ttattttttt ctgttttgac tttttcttta
    24421 agtatctctg atgataaagg gtcgagtccg gcagttggtt catcaagaat aataatttta
    24481 ggacgaaaca gaaatgcaag acaggcactc actttttgtc ttgtgccacc tgataaagtt
    24541 cccatacgtt tatgaaaaat agatttcata ttaaacgcat cgagtaagtc agtgtctgca
    24601 tgattcattt gctctttacg catatccata atcatttcaa acacctgacc aatattcata
    24661 ttttcgggat aacgaccaat ctgcggcata tatccaatct gactgcgata gctgtcgtca
    24721 ttcttaatat ctgtgttgtt aacttttatt gtgcccgatt cgggcaacac cattcctaaa
    24781 acagatttaa taaatgttgt tttaccagaa gcattaggtc caagtaacgc aacagagctg
    24841 ttgttttcaa gctttattga tatatctttt aataccctga gtttaccaaa agttttgttg
    24901 atgtttatgg tttctatcac agtgctacag gtttcattaa tggtttatta tctactaaat
    24961 tttccggtgt aatgcttgga ataatttttt cgctgcggtc aagtaaaaac acaattaaac
    25021 ttcttataaa tattgaagca ataggaatat tttctgtaac catcgaaaac agacttaccg
    25081 gtctgtaggg tacatctcca acaccatctc ttttcaaatc ataaccttca tatttatccc
    25141 agtagttacc atcaaagtta tttaaaacca atgagccatt ggttgcaaca tcaaatgtgt
    25201 ttccgttaaa attattatcg tgaactgtgt tgttactaca gcttgcctga acacgcaagg
    25261 catatccatt tttatagaaa ctgttttttg tgatatgaag tctgtttgtt ccttccatca
    25321 aaactccggt tgtgttgttt tcaaacatgt tgtcttttat atctccgtca gatatttctt
    25381 ttaacaacaa accataggca gaagctcctc tgttcatact aaataaatta taatacattg
    25441 aaatatgatt ggaaaacatg acagcaacac cagcaccatt atcagtaaat ttattgtcta
    25501 tgtatgaatc tttgtttgag aacataaaat gtaagccata acgtaaatta ttataactgt
    25561 tatttttttc aatatgtgag ttttctacaa attcaaaata tataccatct ctatgaccag
    25621 aaatgtcatt attttttata gatgcatact ggcacttcca aagatgaata gcattgccgg
    25681 tagattgttc atcagttccc ggtgttccac gaatggtatt attttctatt ctgaaattat
    25741 cacaattggc aatatgtatt gcaaaataag agttaatgag cgtattgttt ttaattagaa
    25801 actgccggct gttcatgact ttaataccgg ccaaatcttt catagaggaa taacctgaat
    25861 ttttaattgt tagtccggta actaatacac tgtttgcatc aattgttaag acctcaaact
    25921 tattttctcc atcaaggaca ggattgtcaa caccaatgag tgacagcgtt ttttctacta
    25981 ggatgttttg ctctttataa agacctgaat ggataaaaag tgtatcaaaa ggtttagctt
    26041 tagcaacccc ttctttaatg gacttaattg aaaatttttt tccgattttt aaaacacctg
    26101 cctgcgattt gcctgaaaaa aacagaagaa tcagaaaaca ttttacaaca acaaacagtg
    26161 ctctatttaa tttcattctg aacaaaattc cagtcaacta atacaccacc atcatctttc
    26221 aatagttttt cggcttcttc tttagtagca aaagcagcaa agccgctatt cattggcgta
    26281 cgaaggttgt cgctttttaa atagacggca tcagctgcat tcatcaaatc aatctgatca
    26341 aaataattta caacatataa ttttgcagaa cctgcatcta cgttagatga ttgtctgctc
    26401 cattgcaaca tgcagttaat atcatcaaat ttatatatac gtcctttatt tgatattatt
    26461 tctgcaccaa aacgagtatc tgctatgggc attttgcagt gtacacaagc atctttaccg
    26521 gcaactaatg gttcaggctt tgttgtacat gatacaataa gaagagaaat aaaaatgaat
    26581 gtccacctca tacactttat tatttaagat ttactttttc ttaatcccat tgcccagata
    26641 atagcacaga taagtgcaga gccaatgaca atatatccac ctacatcagg catagagtgt
    26701 gcatcaaaat tcaacaattg tttatgtcct aaaattggag gctgataaac taatccggga
    26761 attttaatag gtgcattagg atcaagatta tgtccataat cataacccca ctgatagaaa
    26821 tcgtagagcg caagaatgcc acatataaca gaaactacga ggtaactaaa caatatttta
    26881 cgactaccgg taatggctat cactaaccca taaacaatga atgcagctac aatatatttc
    26941 aggaatgcaa attccggaaa catctctacg ctaatttttt tcatgcctat atagtggttc
    27001 aaaccattaa taacctctac gtcacccgtt agtttatcga gccatatttt cattaccaac
    27061 ccttcaggat attgtggggc aaacaaatca attcgccaga tgggtacgaa aaatgaaata
    27121 gctataagta atgaagagat agcaatgata actcttgata caccgtttat ttttgtcata
    27181 aaaatttgta tttaatttta cctcctaaga attaaattat ttatcaggtg gcaataaaac
    27241 tgcatcaata acatgtatca ccccatttgc tgcaggaatg gtagcaatga tatttgcctg
    27301 attgttaatc attattttat tgtctttatt tgaaatagta atgtttttca aattagcctg
    27361 attgattgtt tgtccatctg taagcatctc tggtttaaaa acaccaagtg aaacatgata
    27421 ctcaagaata tcctgaagtt tagctttatt ttcaggtttc attaaatcat ctactgtacc
    27481 tgctggtaac ttattaaacg cttcattagt aggtgcaaaa acagtaaatg ggcctgcatt
    27541 tgaaagagaa gttacatagt cggcctgttt gagtgctgct actaaagtag aatggtctgc
    27601 tgagcctact gcaatcttaa caatatcttt ttgcgaatca tcgtctataa cagcttcctg
    27661 tccgccagct tgtgtttctt cagtggttgt tgttgattgt tggttttctg ctgcgggagg
    27721 attacagcta aatgctactg atgcagcaaa cattactccc attaataatt ttgtgttcat
    27781 aataatcttt agtttttata aagcacctcg ttggtagtga acaacgaggt gcgttgtgtt
    27841 atatttatta aattaaagat ttagttgata gggggttgat ttgtacctgt gctgaaagtc
    27901 aacggtacat tagaattagc gggactgatt ctgatgtagc cttgcatttc ctgatgtaat
    27961 gcagaacaga agtctgtaca gtagaatggg aatacaccta ctctgtccgg tttccacatt
    28021 aaggttgatg tttctcccgg catgattaat aactcagcat tattagcacc ttttacagca
    28081 aagccgtgtg gaacatccca gtcttgctct aagttagtta cgtggaagta aacaacatca
    28141 cccaatttaa caccttcaat attatcagga acaaagtgtg aacggataga agacatataa
    28201 acatgcactt catttccttt tctttcaact ttagcttctt tttcaccttt cgttacgtaa
    28261 gggtgatgat tttcttcaat cttataaatt tttgtagaat ttttagatag taattctgca
    28321 ggaatagctt ctgcatagtg tggctcacca attgttggga agtctaatat aagtttcatc
    28381 ttatcgccac tgatatcata aagctgtgca ctctgtgtca actccggacc tgttggcaga
    28441 taacgatctt ttgtaatttt attataagca attacatatt taccccaagg ctttttagtt
    28501 ggaccacctg ggacgcataa gtgaccaaca gaataataag taggtacacg gtcaagtact
    28561 gataaatcac tcaaacgcca tttaacaatc tctgatgata caaagaatga agtataggca
    28621 tttccatttg catcaaactc tgtgtgcaat ggtccaagac ctggcttttt aacttcacca
    28681 tgtaatactg catcatattt taaaacaggt aagccatcat aatcgccttc aaaattttta
    28741 gcgtttattg cttcttgcat ttttgtaaat gagaatacag gaataactgc agccaattta
    28801 ccgctaccaa caatatattc acctgttgga tcagtatcac aaccgtgtgg tgattttgga
    28861 caaggcatca gataaaccat atcaggacaa tcttttggat cgagcatgat tacctctttt
    28921 tcaatagttg aagtagctgt gtgtgtttta tcatcaaaag tattgtgagc gtaagaaaca
    28981 ctctttttaa caccctttcc ggcttttaca tattcttcgg cttttttcca gttaacagcc
    29041 ataataaagt ctttgtcaaa cttcgaggca ttaacttcaa gtagcgtatt tgctctttca
    29101 gaattgtaac aactgaagaa gaaccaaccg tgagatttac cttttcctgc acgagacaag
    29161 tcaaagtcaa caccgggtag taatatctgg aatgcaattc ccatttttcc tgattcttta
    29221 tctacattga taaagcttac agtaccttta aaattctctt tataagtatt aatcggaacg
    29281 tctgttgttc cattagtcat cggtacacta aaacgggtac cggcaactac atactcagaa
    29341 ttttcagtaa taaatggtga ggagtgattt cctgcactgt taggaatttc aatgatttct
    29401 gctgttctga aagttttcaa atcaatacgt gcaatacgcg gagtgttgtt tgcattgcca
    29461 aaagcccagc gaccatcatg aataccatca gtttctgata aagaaatgtg gtgcaaatca
    29521 tcccagggaa caaatccatg tgaagtgttt agcatagcct tagattcttc gctgtagcca
    29581 tatccttttt caggatctac agaaaaaaca ggaattaccc tgaacagtct tccagaaggc
    29641 aatccataaa tactcatttg cccactaaaa cctcctgata cgatgttgta aaactcatca
    29701 tatttaccgg gagctacata ggccttttgt gcagcatcac ccgatgtggc tgtactcaca
    29761 tttttaggct tgcacgatgg cattacagca attgtaatta cagctgctga tgccaataat
    29821 acatttttaa tttccgattg tttcataata tttaatttat tttatagttc cttactttac
    29881 accatcattc tcacgcatgt actcatagac atgacgtgca tcatcatcac tcaacccttg
    29941 attaggcata cgcaccaaac attcttccaa tagtcccttt gcaatagcat ctttatctaa
    30001 catttcatca gggtttgtag aaaagttcat aatccactct acagatcttt ttccggtaac
    30061 acctttccag cccggtccca caagtttctc atcggttaac ttatgacagg ctgaacattt
    30121 tacatcataa acggatttac ctgcatctgc catttgttta tcaagttttg gatctacttt
    30181 tacatcagta aactttccaa gaccctttcc atcatctgct accttagtgt cttctttttg
    30241 tttttcttct tcgtaatact gattcatttc ttttgactca gcagcttttt cttcttttgg
    30301 cgcagaaccg ccacaagcag caaaaagggt tatgcccatt ccggcaatca ttaaattgat
    30361 taagtgtttt tgaattgatt tcatattgat gatttgaatt aaaattttac gtgacaaaag
    30421 tataaataat taacagggtg ttcaaaataa gttaatatga ttgatgtcac ataaactatt
    30481 gataattaca tgtagcgtaa actgcttttc agcatgtttc cttaatgaat ctaaaccccc
    30541 taaaaaagta tcaggataaa ataaatatac tgtgtgtttt gctgtccgtt ttagcaatac
    30601 attttagcag aaaacagaaa tcaaccaggt atagagtaat gcaacaacta tagctccagt
    30661 aataatatat aaagctctca aacggtcttt ctttttacaa tgtctgtcat caatagcatc
    30721 aagatttcca ggttcgcgaa aattatgcag actgaaaatc gcaaacagca cgcaaaatct
    30781 gctaacaact tcaacgaaca gaattaatgt tgctaatgtt gtcaggtaaa ggagataaaa
    30841 aatccaaatg ctatgaatga aatgaacagt tattgttaat aatatactga tcagtagaaa
    30901 cgaaatcaga aatttttgcc ttattctgat ctcagcttca cctatgttac aacacccggc
    30961 tttatactga tttgtgtaca ttgtatctat tattaaacct taacatcaaa gttccattat
    31021 tgttctgtaa aaatattgaa tgaagtgaaa atattataga ctctctttat ttactttcac
    31081 agccaaaata gggaaagaga atgatgaaaa aaacggcagt tatttttttt attttattca
    31141 gtcagttgat aactgttttt ggtcagtcgc aggattcttt gatgcttcga aaaatttaca
    31201 atgaatcgtt agagcacgga caggcatata attaccttaa agtgttgtgt aaacaaattg
    31261 gacataggct tagcggttca ccagaggctg aaaaggcagt aaagtgggcc gaaaaatcaa
    31321 tgaaagattt tggtgcagac acggtttttc ttcagaacat catggtaccg cattgggtaa
    31381 gaggtgaaaa agaaagtgct tctataattt cttcaaaaaa caatcagtca acaccattaa
    31441 atgtgcttgc tttaggcgga agtgttggaa caaatggaaa aacgattgca gccaacgttg
    31501 tggaggtaac ttcattagat gagctagata aattaggtac ttcaaaatgt gcaggtaaaa
    31561 tagtttttat caatcagcac atggatgcaa ctaaaattat gacatttgat gcctacggac
    31621 ctgctgttac tattcgctgg gcaggagcta aaagggctgc accattaggt gctgtggctg
    31681 tcattattcg atcagtaaat ccttcattag acgattttcc acatacaggc acattacatt
    31741 atcctgatac aattaaaaaa atccctgctt gtgcagtaag tacaaatgat gctgaaaaat
    31801 tatcagcaat gcttaaaagc gatccgcaac ttaaaataaa gctgaagatg aactgtatgc
    31861 aacttcctga tgcaccatcg cataatgtta ttggtgagat aaaaggcgca gatgcaaatg
    31921 caggatatct aattgttggt ggacatcttg actcatggga tacaggcgaa ggtgcacatg
    31981 atgatggtgc aggagtggta caagccatgg aagtattaaa cctgcttaaa aaagcaggta
    32041 ttaaaccacg tcatactatt agggctgttg cttttatgaa tgaagaaaac ggaggaaaag
    32101 gcggagagta ctatgcaaaa aatgctctgg aaaaaaacga aaaacatatt gcagctattg
    32161 aatctgattg cggaggtttt actccaagag gattcttttt acaaggtgat tccttgaaaa
    32221 aagaaaaaat aaggtcatgg aaaaatcttt ttgaatctta tcagattcac agttttaatt
    32281 ttgatggcgg aggtgcggac atcggtcatt taaaaaatca aaatactttt ttgttaggct
    32341 tgttgcctga cacacaacgt tattttgata ttcatcattc gccaaatgat gtgtttgaac
    32401 atgttaatag gcgcgaattg cagttaggtg cggcaactat ggctgcgatg atttatttaa
    32461 tagataaata cggattggag taatgctgtt gaaagaaact gagctgaaac aaaatgcagc
    32521 agcttatgct gaactgaaaa agaatatttc ttctgaaacc acaccaatat ttttagacag
    32581 aaaaaaagag attttttctg attatgatct ggcgacacgc agggctgcat attatcgctg
    32641 gaaaagcaca gaaagcctcg acaaactcct catcgccttt gaggccaatg caattaaagc
    32701 aggaataaaa gttttatggg cagttgatac tgctcaggca catacggaac tgaacgctgt
    32761 tattgataaa tttcaacagc ataaaattca tatccacaca tgctacaacg agcttgaaac
    32821 ggaaaaatta aatcagcctt cacctaacag tattaagaac aaaataagta tttgtgatgc
    32881 aatttttgtt tcagccgaac cggcaatgat tgcctgttta tctgataaaa atttttatga
    32941 aagtgatgta aaaataatat tagcatcggt tgacagattg atttcttctg tcaatgaact
    33001 tgagttgctt atgtcgctac aatcgcttca aagaaacaat caaagagaat ctttttcttt
    33061 aatttctaat gcaggaaata acagccataa gcaggaagtt tatgttttga tttttgataa
    33121 tggaagaagc aaattattag agagtcagac agaaaggcaa atgctacatt gcattgattg
    33181 tggaatatgt cagacagtat gtcctgtaac agaaagtatt ggcacaacag gaggcaatga
    33241 aaataattac ggtccgccat catatatcag agcggttttt gatggtggac aacgtgaata
    33301 tggacactat gcaggattat gcaatatgtg tggtaaatgc aatgtacact gccctgtaca
    33361 tattgacttt aagaactctt ttctacatct gcgtcatgag aatgtgcagc agaaacaccg
    33421 ttcgcagaag gagcgacttt tttatatggt ttggaaaaaa acgatgctta aacgagagtt
    33481 tatgaattgg aaaacggtta atccgttacg ttatgctgta gaaacacttt ttctgaaatc
    33541 aaaaacagga gtacgaaaac ttccacagcc tgctgccaaa tcatttaatc agatgtggcg
    33601 cgaaaaaatg ggaatgaaat aattttattt cagaattttt ttcagatcag gccttagcct
    33661 gcttttgtaa acagcatgtg cttgtttttt aacacgccct ttgcgaagtc gtttttgttc
    33721 ttcttcagga aggttaataa tggctttaca ttctggagta cagcaacctt cataagttgc
    33781 tttacacgat tcgcattgaa taaacaataa gtggcaatca tcatttgcac agtttacatg
    33841 tgtatcgcat ttgcctccgc attgatgaca cgtactgaca atttcatcac ttattctctc
    33901 acccattctt tcatcaaaca caaagttttt acccagaaat ttattttcta aatgttcagc
    33961 atttacttct cttgcatatt gaatgatgcc accataaagt tgatttacat ccttataacc
    34021 atgatgctta aaataagcac ttgctttttc gcaacgaata ccaccggtgc agtacaataa
    34081 aattttatta tccttattat cctttgtcat ttcaattgct tgtggtaaag catcacgaaa
    34141 tgtatcagca tcgggtttta aagcatcttt aaaaaatcct acttcacttt cgtaatgatt
    34201 tcgcatatca ataacaatcg ttcctgactg ttgcatagcc tcgttaaatt cttttgcaga
    34261 aagatgatta cccacattag taacatcaaa tgtattgtcg tttaaaccat cggcaactat
    34321 ttttgatttt atgcgaacga taagtttgta gaacgacttg ccattatctt caacagcaat
    34381 ttttaatggc atattcttaa gctgctcctg cgcttcaaga taatttttaa acaattccca
    34441 gttttgttca ggaacgctta tttgtgcatt aatgccttcg gctgctacat acactcttcc
    34501 gaatatgttt aaagcttcga agtcaatata aagacgattt cggaaatttt caacatcctc
    34561 aatttttaaa tacctataga aagaaacagt tttcctgtta aatgtttctt cggtaagacg
    34621 ttttttgagg agttgcttac ttacaaggtt tcgtaattgc atgttacgtg ttgtaattaa
    34681 acagttagat ataatgatta accttctttg gtttcttttt tcttgatttt attgtaaaaa
    34741 aaataaaagc cgacaccaac catgacgtat ggaacagcca ttaaataaag tattccctta
    34801 ttgagacctt taccggcttt gtatccttgt tcaacgctgc tttctgcacc tgccctgcaa
    34861 acgctgcatt gtgcatttac attttgaaag gtaagtgccg acaagaatat aattaataaa
    34921 attaaaagcc gtttcatatt atacagttaa tgttcgttta acggcaacct gctcatagcc
    34981 ttcaataatg tcgcctactt taatgtcgtt ataaccttct atatttaatc cacactcaaa
    35041 tccggcagaa acttctttta cgtcatcttt aaaacgtttg agtgatgcaa gcttgcctgt
    35101 ataagctact ataccatcac ggataactct gatggcagta tttcttgtca tttttccatc
    35161 aagcaccata caacctgcaa tagttccaac ttttggtatc ttaaatgttt cacgcacttc
    35221 aatattgcag acaatctttt cttcaaactc aggtgcaagc attccttcca tagccgcttt
    35281 aatttcatta atagcgtcat aaataatgct gtaaagcctg atttcaatct cttctttttc
    35341 tgcaagtctg cgtgcattta ctgaaggtcg cacctggaaa ccaacaatga tggcgttaga
    35401 tgctgatgct aagagaacat cagattctgt aatggcacca actgatttgt gaatgacatt
    35461 aacctgcact tctgctgttg aaagtttaag cagcgaatcg gcaagagctt caatagaacc
    35521 atctacgtca ccttttacga ttacattaag ttctttaaag tttccgattg ccaaacgtct
    35581 gccaatttca tcaagtgtaa tatgtttgtg tgtacgaata ccttgttcgc gctgtagctg
    35641 caatcttttg ttggcaattt ctcttgcttc tctttcatca tccatcacat gaaactgatc
    35701 accggcctgt ggcgcaccat taagtccaag aatcaaaacc ggggttgctg gtcctgcatt
    35761 atttctttta tttccccgtt cgttgaacat ggctttcaca cgaccactat aacaaccggc
    35821 aagtactaca tctccaatct tcaatgaacc ggattgaact aatactgttg caatgtagcc
    35881 tcttccttta tctaatgatg attctactac agtaccaatt gcatttttat ttggattggc
    35941 tttaagatcg agcaactctg cttcgagtaa aactttttca agcaacaagt caacattcag
    36001 tccttgtttg gcactgattt cctgtacctg aaatttacct ccccagtctt caaccaatat
    36061 attcatttta gaaagctgct ccttaatttt ttcaggatta gctcccggct tatcaatttt
    36121 atttattgca aaaacaattg gaacacctgc tgcctgtgca tggtttatgg cctcaatagt
    36181 ctgcggcatt acgctgtcgt cagcagcaat tacaataatg gcaacatcgg ttattttagc
    36241 tccgcgtgca cgcatagcgg taaatgcttc gtgaccaggt gtatcaagaa aagctatttt
    36301 cttaccactg tcaagttgca cttcataggc tcctatgtgc tgtgttattc caccggcttc
    36361 acctgaaata acattcgttt ttctgacata atccaataaa gaggtcttac catgatctac
    36421 gtgacccatt actgttacaa tcggtgcgcg tggtttcagg tcttctggtt tatcttccac
    36481 ttctttaatg gcttcctgta cttctacaga aacaaattca acagttgcac caaactcttc
    36541 ggcaacaaca cttaatgttt ctgcatctaa gcgttggttg atggatacca ccatacccaa
    36601 gctcatacag gttgatataa tctgattaat cggtacaccg agcaacttcg acaattcgct
    36661 ggccgttaca aactcggtta ccttaatcac tttcttttca ttactttcct gctcaagttg
    36721 tgattgtaat tcttcctgag cttgttcgcg tttaagtttt ttaagtttag atgatttgga
    36781 ctttcctcca ccactcagtc gtgctaatgt agctttaatc tgatcctgaa tttccttgtc
    36841 ggtaacttca ggcttagctt ctgccggttt attccggaaa tcatttctgc gtttaaaatc
    36901 accatgtccg ccacccggtc tttgtccgcc acctgtacgt ggtgcaccac cctgatgagg
    36961 tggcccacct gcctttgcga tgtcaacttt cttatctttt ctttttctct ttttctttct
    37021 gtcgtcttct gatgatgtgg caacaggctt tttcttaggc ggatcaacag gcaggtttat
    37081 ctttccaaga attgttggtc ctgttaattt ctctctgtct gttctgaata attccggagg
    37141 tgttgtatca tcagcttttt gttcagcttc tgtagtatca gttttgtcgg tttcttcagt
    37201 taccggagtt gcgtcaatga ctacttcggg ctgtgtctca acaatggcaa ttggttcctc
    37261 ctcctttgta acagcagcta cttcttcaat ttgttttacc tcttcttctt cttttttctt
    37321 tgtggttttc tttttcttcg gttcttcctc tatagccaca acttctgctt ttttctttgc
    37381 agtagttttc tttggttctt cagcagtgct atcttctttc ttaagcgttt ttttcttttt
    37441 cttttctgta gcttcaagat cgatcttacc ttgaatttta ggaccttcaa gcttacttac
    37501 tttagcttta acaacttcag gttcttttgc tttttctttt gtttcaaccg gtttatcaac
    37561 ttcagcaaca ggtgcaggtt cttctgcttt aacaaccttt gctttagttg ttttctcttt
    37621 ggtttctttt gctttagcag tagcttgttt ttctgttgcc tgttgtttta tgctaagaag
    37681 tttatcggcc aattcatcat cagtttctga ttcagaacca gatttgcctg aatcatcttt
    37741 tacttcaatg atggtgtttt cgcgtttttg tttttcgcgt gtaatctcat tggcttcctg
    37801 acgtgcatgc atatcaggtg caaactcttt cgtcagcaga gaaaaagcat cagcactgat
    37861 tttggtgtta gggttctttt ccacttgaat tccgttttta tttagaaatt caataatgtg
    37921 gtcaataccc acacccaaat ctttggtggc tttacttaag cgtacgtctt ttttctcgtc
    37981 tgacattaaa cagtctatgt ttttatctta aaaattaata gcagcatgca tattggctgc
    38041 aaatttagct tattcaaact cttcattcag aattcttaca acttcacgaa tagtttcttc
    38101 ttcaaggtcg gtgcgtttta ccaaatcatc aatagagatg gcaagtacat cctttgctga
    38161 gtcacaaccg attttcttga actcctcaat aatccatggt tcaatttcat cagtaaactc
    38221 atccaaatca acgtcttcat cgtcttcaac atcagaatcg cggtaaacat caatctcaaa
    38281 tccggtaagt ttaccggcca acttaatgtt gttaccacct ttgcctatgg ctaatgaaac
    38341 ctgatcgggt ttcatatata cagcagcttt tttagtgtca tcatcaagct ttatttcaga
    38401 aatttttgcc ggactcaaag cacgttgaat gtaaagcgat aaattgtttg tgaaatttat
    38461 aacatcaata ttctcattgc gtaattcacg aacaatacca tgaatacgcg aacctttcat
    38521 gcctacacaa gcaccaacag gatctattct gtcatcatac gattcaacag caaccttagc
    38581 acgttcacca ggctctcgaa caatattttt tattgtaatt aagccatcaa acacttcagg
    38641 tacttcaagc tcaaacaagc gttctaggaa tacaggtgct gtgcgtgata aaataatttt
    38701 tgggcttcca ccttgcattt ctacccgaag aactacagct ctcacagtat cgcctttttt
    38761 aaagaagtcg gcaggaatct gttcgttttt aggtagtgac aattcattac cttcatcatc
    38821 cattaccatt atttcttttt tccaaacctg ataaacttca ccggtaacaa tttctcctac
    38881 gcgatcttta tatttctgat agatggattc tttttcaagc tccataatac gggcaactaa
    38941 attctgacgt gcagcaagca ccgctcttcg accaaagtca tcaagaagaa tctgctctgt
    39001 aacatcttcg ccaacttcaa agtctgattc aatttttaat gcatcgctca atgctatttg
    39061 tgtgttgtca tcttcaacca taccatcttc tacaatggtt ctgtttctgt acatttccac
    39121 atcaccttta tcggtattga ttacaacatc tactttgttg tcttcgccat atttttttct
    39181 gaccatagca cggaatacgt cttccatgat actcattaat gtggcacgat caatgttttt
    39241 agagtcttta aactcactga atgattctat aaggacggca tgatccattg tattgatgta
    39301 ttatttaaat tctaatttag tttctttaat attttgcatc ggtaaagtag ttgtaagttg
    39361 tttttcaact tttttaccgc ctattttttc tttaattgtt tcaagtaatt gtaattcctg
    39421 ttcgttggca tcaacaagaa ttccgttatg ttgcttacca tcgttaagta taatttttac
    39481 cggacgacct attcgtcttt tatactgacc aataactttc agaggctgat ccattcccgg
    39541 tgaacctact tcaaactctt tattttcaaa cacacctgat gattccatct ccttgtaaag
    39601 ataacgattg agtaatacac attcatcaat ggttatgcct gccggcttat caaccaaaac
    39661 agttattttt ccgggagtaa cttttaaatc taccagaaac aggtccttat cagcaatata
    39721 gaccttaata agttcatgta tttgctctgc aatcatcttt aatgggtact gaataaaaaa
    39781 ggggacattg tccccttcac ttacctaata cgccacaaaa gtataaaaaa taactaaata
    39841 tgaaatgaaa agtatatttt taccattgat aataccttaa accaaaatac tatggcagat
    39901 aacctaaaca tagagaaagt cctttttttg gacattgaaa ccgttccaca gtttgaatct
    39961 tatggcagtt taaacagtgc tgataaagaa ttgtgggata ataaggcact tcatctgaag
    40021 aagacagatt ttgatacagc agaaagtttg tatccaagag caggcattta tgcggagttt
    40081 ggtaaaatag tatgtatttc tgttgggtgg tttgtgattg aaaatagtaa gagaaacctt
    40141 aaggttaaat ctttttatag tgatgatgaa acaaacttat tattgaaatt cagtgagtta
    40201 ttaaaaaacc aattcaataa atcatcacat ttgttgtgcg cacacaatgg taaagaattt
    40261 gactttccgt atctgtgcag aagaatgctt ttaaacaaaa taaaaattcc ggatatctta
    40321 aatattggag gtaagaagcc ttgggaggtg caacatttag acactatgga catgtggcgt
    40381 tttggagatt ataaaagtta tacgcctctg aaattactga ctcacatttt taatattccc
    40441 agtcctaaag atgaaattga tggcagtgat gtatggcatg tttactggaa agataaagac
    40501 ttgaaaaaaa tcgcaaagta ttgtcaaaag gatgttgtag ctgtggctcg attgatcctt
    40561 gcatttaaaa atgaaagtgt attgaatgat tcggaaattg aaattttaaa ctaaccgttt
    40621 tttctgaaaa tataaatcag actggaataa tttcctgtat aaatagcggc atgcatattt
    40681 gaatgaatac ctctgtaaaa tgctttaatt atttgtatgg gttttgatga tttagcctta
    40741 taacgttcgc ttaataatga aacataagtg gagtcaaaca acattggttt tgtgtcaagc
    40801 aatgtaaaat taaaagattc aattaagctg gttaaagcct ctggcgtaaa atgataaaga
    40861 tgtcttggca tatcataagc agcccaaaat tctttataaa attcagcatc tttagaagca
    40921 ggatttggga ctgcaataat cagaatacca tcttttttaa gtgataattt gatattgttt
    40981 aatgtttttt ttagagtatg aacatgttct aatacatgcc aaagtgtaat acagttaaac
    41041 tcttcattac cagaagagta aaaggattcg atgggtaata agttggtgtc aaaagtttct
    41101 tttgcaagtt taatagcctc cggtgatggc tcataacctt tagtttggaa accggctttt
    41161 aagagtgctt ttacaaagta tccattacct gcacctaaat ctgcaacata ggagttttta
    41221 ggtatcagtt tttttaccaa tctaactttt tgatttacag caatattcct aacaattgga
    41281 tacaacttat ttataattcc tttactttta ttggtgtggg acacatagtc atcagactta
    41341 taataagcac ctatttctgc ttcgtccggt ctgggggatg tgaacaacaa tccacatcta
    41401 acacattgat taatagtaaa atacgagcct gaataaccaa aatctttaca ggtcaagtat
    41461 tcaattttat cttgagtccc acaggaagga cattcattaa gtaattccat tcaggggcaa
    41521 aaataaaaaa agaccaccat caggtagtct ttagtttcac gtgaaacaac acttttatta
    41581 tcggcctaaa tagaccatta agatagatat gtcagatgga gaaacgccac ttattctgga
    41641 tgcttgccct agtgtttgtg gtttaactac agaaagcttt tgacgagctt ctgcactcag
    41701 tgatttaatt ttatcgtaat taaaatcttc gtgcaaaata agattttcta atttgttaag
    41761 tttaactgta tgctcaaact cttttgcaat gtaactttcg tatttcattg ctatttctgc
    41821 ctgctcaatc acttcgtttc ttaattcagg agatataaag gataattttt ctgataaggt
    41881 agaagaatga tttataagat caattaaatt tattccagga cgatttaaaa cctgaataat
    41941 ttttactttt tgtgaaagtt ctgcagactc ctttgaatta aggtaggcat taatttctga
    42001 aggcgaaata ctttctgttc tgcaagcttc aattattctg tccacagcct gttttttttc
    42061 tgttacacgt gaaaatcttt gagcatctgc taaaccaatc tgatgactta caggagtcag
    42121 cctgatatct gcattatctt gccttaaaag cgtccggaat tctgcacggc ttgtaaacat
    42181 acgataaggt tcatcagttc cttttgttac caaatcatca atcagtaccc caatgtaagc
    42241 gtcagagcgt tttaggatta atgggttttg attattaatt ttaaggtgag catttattcc
    42301 tgctattaaa ccttggcagg cggcttcttc atagcctgtg gttccgttta tttgtcctgc
    42361 aaaataaaga tgttctattt tttttgtttc taaggtcagg tttaattgaa gaggagggaa
    42421 gtaatcatat tctatggcat atccgggtct gaacattttt acgttttcaa gtccgggtat
    42481 tttttttagt gcggcatatt gcacttcttc gggcagactg gatgaaaatc cattgagata
    42541 aatttctact gtattccagc cttcgggttc tataaatagc tgatggcggt ctttgtctgc
    42601 aaagcgattg attttatctt ctattgaagg gcagtatctt ggtccaagtc cttgaattct
    42661 gcctgtaaac attggcgatt tttcgaatcc ggtttttaag atattatgta cttcatcgtt
    42721 ggtgtaggtt atatgacaac tacgttgtgt tgtgaggggt atggtgtctg taaaggagaa
    42781 tttacctggg ttttcgtctc ctttttgttc ttctaatttt gagtaatcta tactgcgacc
    42841 atctaagcgt ggaggcgtac ctgttttcat tcttccactg acaaaaccta ggctaactaa
    42901 ctgttctgtg atgcctgttg cagctttttc tcctgaacgg ccaccgccaa agcgtttttc
    42961 acctatatga ataatgccat ttagaaaggt tccattggtt aataccactg ctttgcatcg
    43021 tacctgatgc cccatgctag ttacaacacc ttttagcttg ttatcttcaa taattaaagc
    43081 actgaccata tcctgccaga agtcaacatt aggtgtgttt tcaagcatct gtcgccattc
    43141 tgaggcaaat agcatcctgt cattctgtgt acggggactc cacatagcag gtcccttaga
    43201 gcggttaagt attctgaatt gtatggctga tttatcagag actatacctg aatagccacc
    43261 taaagcatct atttctctaa caatttgccc ctttgctaca ccgcccatag caggattgca
    43321 actcatttgt gcaatggttt gcatgttcat ggtaatcaat aaaactcgac tacccatatt
    43381 ggcagcagca gcagcagctt cgcaacctgc atgaccggcc ccaaccacta cgatatcata
    43441 ctcttgaatc atataatagc acaattaaaa accaaaacga ttggtttcac gtgaaacatt
    43501 taaaatatag ttgaattaaa ttcgtaaatc cctttaaatc aaaaggttcg caaagatagg
    43561 tatttgtttt ctgccttggt cattttttgt ttatcggcag gctttttatc actatatcca
    43621 cataaatgaa gtacaccgtg tatcatgaca cggtgtagct catcttttat gctgtttttc
    43681 aataatttag cgttttctct aactctgtct atgctgataa aaatatcacc tgaaatagta
    43741 gcatcttctg tgtaatcaaa ggtgataata tctgttaatg ttttgtgatt cagatatttg
    43801 atattcattg agtacaataa ctcatcagag caaaaaataa aatttaaggt acccggtttt
    43861 ttacggtgct ttttaataca gcccactacc caagaagata ttgcatcctt gtcattcaag
    43921 cgaaattgta catcttcctt aaaaaaattc acttcagttt gcatcactta gccggtacag
    43981 gaggctccat aaccgtactg cattgcttgc aagtcctttt ttcgagcgaa ttgtaaaaat
    44041 cgttcatcac cacaggtaat tgatttacaa tatctttcaa tgggaaaaac tcttcatata
    44101 gtttgtgatt gcatttttcg caaaaccaca tgcaggcatc catttcatcg ggcttacgat
    44161 agcgctctac taccaaacca atagtgtttg cacctctttg cggactatgt ggtactcgtg
    44221 gaggtaataa aaacatttcg ccttgcttga taggaatatc aaccggcttt ccatcctgaa
    44281 tggtttttac cacaatatct ccttctatct gataaaataa ttcttctgca tcttcaaaat
    44341 ggtaatcctt tcttgaatta ggaccaccga ctatcataac aataaaatct ttattgtcta
    44401 tataaacttg tttgttgcct acgggaggtt ttaacagatg acggttttct tcaacccact
    44461 gctgaagatt aaaaggtttg ctgactgcca taatttttaa gattgaattt atttttgaaa
    44521 tagattgttg agtttcttag atacttcctc ggtaaattct tgtgcttttg tgctaaggtc
    44581 ttttgccgag ctttcaagat catcctttaa agtttcgaat ttttgtttta caccgtcaga
    44641 taagttacct ccggtgagaa ggatgtctat ttgattatgt aacaaataag gtaatttttc
    44701 ttttagtgct gcagatacag cttcaattgc cttggcagct tgctcctgat taatattagc
    44761 agccttactt acctgatcta ctattggatt tgacatgatt ttgtgttttt ttgttttcca
    44821 aagttatggt aaaaaaatgt ttatcaaatt aagtcaggta catttgcaat atgaatctgg
    44881 atttaacgaa taaaaaagcc cttgtttgtg gcagcacaca aggcataggt aaggcagcgg
    44941 caaaagagct ttgtcttctt ggggcaaagg ttgttctatt agcaagaaat gaacaaaaac
    45001 taaaagctgt agctgcggaa ttaaacactt tttgtaagac aacgccagag tatattgtgg
    45061 ctgatttttc tgatcctgaa aatttaagac aacaggttcg tgcatacata aataaaaatg
    45121 ggggcttcaa cattttgata aacaacgctg gtggtcctcc ggcaggaaat attactgatg
    45181 ccgcagcaga aagttttatt caggcattta aagagcattt gatttgcaat cattatctgg
    45241 cactggagtg tatagccgga atgaaagaga tgggttacgg aagaattatt aatgtcatct
    45301 ccacctcagt gaaacaaccc attaagggac taggtgtttc aaatacgatt agatcagcag
    45361 ttggtaactg gagtaagact atgtcaattg aattggctcc attcggcata acagtaaaca
    45421 atgttttgcc cggtgcaaca ctcactgcac gactggaaca gattattgca gataaagttc
    45481 agaagacggg aaagacagaa aaagaagtta ttgagtcgct gacatcagaa attcctgcca
    45541 aacgatttgc caatccggaa gaaacggccg ctgcaattgc atttttagct tctccggctg
    45601 cgggttatat caacgggacc aacgtagttg ttgatggtgg ccgtacagca tgcctgtaaa
    45661 attattgata aagtagaaac ttaattttat ttaaaatctg atcgttgttt aatggtcttg
    45721 ataaagtatc ggtagcaccg gcatcaagac atttttcttt ttcctgagca tcagagcctg
    45781 aaagaacagc cagcacagga atatttctga actgcgctgt tttacgtatt tcggcaatag
    45841 catcaactcc attcattacc ggcatcagga catccattaa tacaatgtca acatgcggat
    45901 ttttttcaag tttgtttata gcttctttac cgtcataagc acaaataaga ttggctcctg
    45961 cattttcaat taatgtagtt aatgcataga tatttcgtaa atcatcatct gccatcaaaa
    46021 tggtttttcc gcttaatgat gactcatgaa aagtatgctc aacaactgtt tctttctttg
    46081 taatatttac aggcgcttgt aactcattca ttacattttc gagctgcttt ttgtttaaag
    46141 gcttgtgatg ataggccgtt atattaaact cattaattaa accttctgta ggtttaagct
    46201 cactgatgat atggatagga atatgactaa gcttttgctc cgaacgtata tttttaagta
    46261 aggttccacc atcaatgtca ggcagattat gatctacaat aatgacatgt ggttggtaat
    46321 tcttagcaag caaaataccg gtttcaccat tctgagatgc tatggcttta tagttaaaaa
    46381 tgtgacaata gtctattaat gttttaagga atgtaatatc cttttcgaga ataagaatta
    46441 ccttatcgaa gggtgtgata aaatttctgt catcggtaaa atcatcatca gggaattttg
    46501 aaactatggt actgattaat ggtgactcat gttttatatt ttcttgtttc tctgtttctg
    46561 cttgtgtgat tacttttttt acaggggtgt catgtgtgct ttcgcatgat gcaaaaaacg
    46621 gaataaacac agtaaacgta cttcctttac ccggtgtgct ctctaaaaca atttcaccac
    46681 caagaatttg aactatttcg cgactgatgg taagtcccag ccctgttccg ccatactttc
    46741 tgcttgtact gttatcggcc tgtttaaatg cttcaaacac ctgcttttgt ttttcaacgg
    46801 gaattccaat tcctgaatct gttacgctta ttgcaataat gtcttcttca aaatgcaggt
    46861 tatgagtctt acaatcttct gttgttgctt ttctgaaatc aagacttact tgtccgtttt
    46921 tatcggtaaa ctttaaagca ttggagataa gattttttat gacttgctcc agacgcaact
    46981 tatcactcac catttccgga taacaattct cccatacttt tacctgaaaa tttatctgtt
    47041 tttcgttggc aaactcttta aacaacaggt taatgtcgtt agcaacggtt tcgatattca
    47101 ttttttcaaa aacaaattcc agctttccac tttcaatttt tgaaatgtca agaatatcat
    47161 taataagcat taatagatca gaccccgact tatgtatgac acttgcatgt tcaatttgtt
    47221 tgtcggtgag gtttttattt ttatttcctg caagcagttt ggcaagaatc aaaatgctgt
    47281 ttaatggtgt gcgaagctca tgcgacatgt ttgcaagaaa tgccgattta aacttattgg
    47341 tttgttcaag ctgtgttgct ttaaaggcaa gtgattgacg cgcttcttca acggcaaggc
    47401 ttctttcttc aagcagatgt gctttaattt caagattggc attggcagct ttcaattctt
    47461 cctgttgctt ttccaattct ttttgtgata cctctaactc ttcagattta atagtcaggt
    47521 tatgatttac ctgcgccaac tcttgttttt gtgctaccaa ctcttctgac tgcatgatac
    47581 tttcttcaag cagttcgcga gtaagtgcat ttgaatatac agcataaata cttgcagata
    47641 cgggttcaat aagacgttgc agataattta atccggaagc agtgagttta tttaaagaag
    47701 caatttctat agcaccaata acacgaccgg atgcaaatag tggcatgaca aaaacagtac
    47761 aaggcatgga cttattttct ccgtcctttt taatgtcgaa gagtaaactt gtttctattc
    47821 tttcgttatt gtcaatacat tttttaccaa ctgatgttta atccactcta tatgtaagtc
    47881 ggtaagttgt tgacttctgc aataaattcc tttaaggtca acttcatgca gcgattcatc
    47941 ttttgtgtct aataaaaata ctgttccatg gcaggcaccg gaatattcaa caacttcttt
    48001 tatcaggtta ttgcaaagca cctgcatgtc gagtgtataa tctttattca aaacactgat
    48061 tcgctcaaga ccttctgaaa tccatttttg cgaagcatct tcatcgctgg ctgcttttag
    48121 cttggaacgc atgtcaagaa gtgctttacc ctgaacatca ttttcgctta atggttcaaa
    48181 gtgatagtca aaatttcccc gaccaatgtt gttggcaaac tctgctgtac gatttaatcc
    48241 gcttacatgc gttttaagtg catctatcat ttcgcctaca gcattctttg gtgttttaat
    48301 actcagttcg ggcaactcgc cttttcctaa ctgcaacaga tagcgtctta ttttcattaa
    48361 tgggttaaca acctgtttgt tcagaaacaa agtaattacg ataatgaaat taattatcac
    48421 catggctaaa cctaaaacaa ctgccatcaa aacaatgaat gactgaatca tttctttttg
    48481 tttggctgta gtctcagcac gcttttttat gttgaagtcg cttagtagtg cagtaatttc
    48541 ctgtacctgt ggggtaattt gcgtttctaa aatttctaca ttattctctg tactaacacg
    48601 agctttctca atcaactgca attcaaaata aattaaacgg tcgtattttt cgaatagacc
    48661 gattagggca agctgttctt tttgttgcca agattcagaa agacgtttaa gttctttttt
    48721 taatgcagga tattgtgttt cattaataaa ctgaagttcg cttatggagg cagttttgtt
    48781 tttgcgaaaa gctacattgg ttgcctggag ccatgaattg tttaccaatt gtgttaagtc
    48841 ggaaattgcc gattgtgagg gtgtagttac ttctgcaatt tcaatggtga tgtttttgct
    48901 tttgtcaata atataggtgc ttataaaggc attggctagg gtgataacaa taagcatgat
    48961 aaaaatgacg taaagctttt tatcaatttt aaaagaccat ttattggatt tcattgctcc
    49021 ggttataaaa aatattaaaa attatacgag gatacggggt aaatggttac ctgaataaaa
    49081 caaaaaatcc cgctaagaga ggcgggattt tgagtgaggt cgcgagcaga ttcgaactgc
    49141 tgtacaaggt tttgcagacc tctgcctagc cactcggcca cgcgacccta tttactggcg
    49201 gcaaatgtaa aaatattttg cttgcggcaa taggaatgaa aatgctgaaa taaatggttt
    49261 taaacgttaa ataaacaaaa ccggttacat taaatttcaa ttacaaatgt tgcttcacgt
    49321 ataaaaccct gtactggagg attgtatttg gttatagcca cttttatttt attacagatt
    49381 gtaaaacgtt ctttcatttt ttgtgcaatg cggctgccaa catgttctat tagttttgaa
    49441 ggaattgcca tttgtatttt aaccatgtca ttgacttcga cataatcaat agtctgtgtc
    49501 agattatcgg agtttgcggc aatactcagg tcggcataaa aagttatatc cacacgatag
    49561 cggccaccaa taacggtttc ttgtgataaa cagccatggt gagcataaca ttcaattccg
    49621 gataaaataa gttggtgcat aaagcaaaat tttggataca aaagtaattc attggcaaat
    49681 gattcttttt atttaatatc gccttttatt tatgaaatat agaaatgaaa attcgtcttg
    49741 ttttaatact cacttttata aactttagtc tcctgatggc tcaacaacct ataacactaa
    49801 atgtgcctca gcctgatcaa gactggccta acagtgttac ctatcatatt tttgtccgct
    49861 cgttttgtga tggcaacggt gacggcattg gcgaccttaa gggcattact gccagatggg
    49921 agtacctcag aaagttaggt gcagaagcag tattaataag tccgattttt gcttcaccat
    49981 cgtatcataa gtacgatgta acagattact ataaattatc accggaatat ggaaacatag
    50041 agtcgtttca atattttata aaagatgctc atgccgaggg atttaaagta attttagata
    50101 taccattaaa tcattgcagc aacaaacacc cctggtttat agaggcagca aaaaatccgg
    50161 caagtacaat gcgaaactat tttgtgtgga gcaacaatcc gcctgcaaca gaaaaagaca
    50221 aatggcatct gccaaaagat gaaaaaggaa atacaatagg aaaaacatac tattatggtt
    50281 ttttcagtcc tgaaatgcct gactggaact atgacaatgc gcaggtagtg gaggaagctc
    50341 aacgcatttt aaaattttgg attgatttgg gagtagatgg gttcagatta gatgctgcac
    50401 agcatatttt taaagatcac aataaaaata ttggcttttg gaatatgatt tcggcctatg
    50461 taaaaagtat caacaagcat acatttttag taggtgaggt ggataattct tcagatatgg
    50521 tagcacccta tcttaaacca ctcgatgcct gctttgattt tgatttatcg cgtgaagttg
    50581 ttcaatcact gaagaatgag aatgcaagta acttccctat gagaatggaa aaaattatca
    50641 cacactataa agcagtgaat aaggattata aggatgctat ttttcttact aatcatgatc
    50701 agaacagggt gatgagtgag gttggaaata atgttgacaa agcaaagctt gctgccaata
    50761 ttttatttac gcttccaggt attccataca tttattatgg agaagaaata gggatgaaag
    50821 gtgaaaagcc tgatgaattt attcgtgagc ccatgttatt tgcacctgaa aaagaagatg
    50881 aaatgcgccc caaatggatg aagcctaaat acagtacgga taaaaccgtt gagtcggcaa
    50941 tggttcagat aaaagaagat cagtcgctat ggaatcatta ttcaagactt atttctatgc
    51001 gcaaagaaaa cagagcattg caatacggtc aatttgaaaa ctcatctttg agtagtaaaa
    51061 gtgtattggc ttattatcgt atctataatc agatgcgtgt tttgataatt aacaacgtaa
    51121 gtgccacagc cgttacacta acaaaagagg aaaaagataa cacgcttata aatccgattt
    51181 atcagacatc aaaacaaact acagttaaag gaaacaaaat tacgcttgct ccatacagta
    51241 caattattct caccaattaa aagatattta aaacttatat tagtcctgaa aggatgtaat
    51301 ttacaagagt agcgtgaagt agtgcccttt cagggtcaag ataatatatt acaatttccg
    51361 ctattattag actaacttca aagcagattc taatctttta agcacctggt tttttccaag
    51421 cagctgtatc attggaaaaa ggtcaacgcc ttgtgcctgt ccactaacaa aaacacgaaa
    51481 gagttgaagt acttcaccgg gtttaagatt atttttttcg gcagcggctt taaaagtctg
    51541 ctctgcaatc tgactgttcc attcatttga tgcagtaaat tcatcagaaa gcattttgaa
    51601 aaaatcttta aattcaggtt tccatttttt agagataact gtctgatcat attccgttgg
    51661 ctctataaaa agatacaatc ctttttcagg catttctttc tcaaactgaa cacgaggttt
    51721 tagcaatgcc acacatgctg ttagaaaact atcagagagg cgcacatcat ccgtagtgat
    51781 tcctgtgagt gatgcaacca taggtttaat acgttttgca atgtcttcat cggattgtcg
    51841 cagcatccat tgagtattgt accattttgt tttttcaggg tcgaagcgtg ctcctgcttt
    51901 gtgaacatga tctatagaaa atgcagcaat gagttcgttc atattaaaaa tttcctgctc
    51961 tgttccggga ttccatccca acatagcaag catgttaaca aatgcttcgg ggtagtaacc
    52021 attttcgcgg tagccgctag acacctcacc tgttgtaggg tctttccatt caagaggaaa
    52081 gacaggaaat cctaacctgt cgccatcgcg cttgctgagt tttccttttc catcaggttt
    52141 cagcaataaa ggaaggtgtg catagtgagg catttgcgca gttaatccga gataacgata
    52201 aattaaaata tgtgcaggtg cagaaggcaa ccactcttcg cctctgatag catgtgttat
    52261 ttccatcagg tagtcatcaa caatatgtgc taaatgatag gtaggcatgc catcactttt
    52321 aagcaacact ttatcatcaa ctaaattaga gtcaaaactg acttcaccac gaatgagatc
    52381 tgtaaaagtt attgtttcat tttcctgaac tttcagtcgg ataacataag gaacattctt
    52441 tgaaattagt tctttaactt cttcaacagt taatgccaat gaattattaa gactcattct
    52501 tgtagcactg ccataagaaa aagtttcttt tttttcttct gcatttttgc gctgttgttc
    52561 taattgttct gcagtatcga atgcataata ggcatttccg gatgcgacaa gttgcatggc
    52621 atagcttctg taaacttctt tccgttcact ctgtctgtat ggtgcatgtg tgccgccaac
    52681 agaaacgcct tcgtcaatgg tgatgccgca ccagtgcagt gcatcaatga tatattgttc
    52741 ggcaccagga acaaagcggt tctgatcggt atcttctata cgtaataaaa aatcgcctcc
    52801 attttttttt gcaaacagcc aggcgtataa tgctgtgcgc acaccgccca tgtgtagtgg
    52861 cccggtgggg ctgggggcaa atcgtgttct tattcttcgc tgcataaaat aaattctgct
    52921 gcaaagataa tcgagaaaaa gttgtgtggt ttatttcttt agtgaagtta tacttgaagt
    52981 gagtttttct tcaatttttt tgtagcggct caactgatca aaatactcag gctttgtttc
    53041 cagttctttt attttgttag tgacaaattt tttgagctta ctaagtgtat tggaagtttg
    53101 catattcata aactttgagc tgctgttttt tgcagcatca gtcaaagcgt caacaccttt
    53161 ttctgtcaca gcaggtgaac tgtgctcgag gaaagttccg aggttttgaa taattcccat
    53221 ctttccaaat ccggatgcta catttaatgc atttatcatg taatcgtaat tttcatcact
    53281 gccatattgt gcataaagtc ctgagagtgt ataaagaagg ttgttgttat tatcggactc
    53341 atattttttt agtgaggaca attgctttgg attttttgtg atgtaatgtt ccaatgcagt
    53401 agtcattaca ttgtatgaag agtcgtttat agcaatatta tctaaagaag aatcatcaaa
    53461 atatttatat aaggttgaca aggcagattc tcttacatgt gctttagcat ctttagttac
    53521 catttgttgt aatttgtttt ttactgtact gtcagctttt gctgctgcac caatatttct
    53581 taaagcaagt gctctgatag aaggattttt gtcgtctaat gcgctgataa taatttttgc
    53641 ttctgcacta ttgccttcat aacctttggc taatgcatca atagcttcgt aacggtcgag
    53701 aaacaatggc gcatgctgat actgatacct ccattcatcg ctggtgtggt tgtccttttt
    53761 agcgcataac aattgttttt ctgcatctac atttatcaat tgtggcttgc tgttgcaatc
    53821 aaaaaataaa tattcttttt gttgtgtaag tgtaacctgt tcgcgtctaa cttttccgtt
    53881 ttcataaata tctattgcaa gaggaagttt atagagtggt gtttcctgaa gattttgttt
    53941 ttgttcgatg atgacggttt gttttttcag agtatcgttc cactgataac tgatatcaat
    54001 tatgggatga cctttagcaa aaaaccactg attgaaaaac cagttgaggt cttgtccggt
    54061 aacagcttca aaagccaaac gcagattatg tgcttcaaca gggctgaatt tattgtcgtt
    54121 taaatatttt tttaatgatg caaaaaaggc atcatcgcca acatatttac gaagcatgtg
    54181 taacacccga cccccttttg aatagctgtg tctgtcgaac atatcttcac gagcattgta
    54241 atgaaagcga ataagatcaa cctgtttgta tttggcttca gcaagatatt gattcaaatc
    54301 atccatgccg cctttgtcag cttcttcacg accgtatttg tattcatccc acaggtattc
    54361 gccataagtt gcaaacgatt cgtttaatgg tatgttgctc cacgactcac acgttacata
    54421 atcgccaaac caatgatgaa aaatttcatg tgcaatcgta ctttcacttg tgccatcaag
    54481 cagttcgcgt tttgttctct gtaaaaactc accataaaga attgcagaag tattttccat
    54541 ggcaccactc acataatcat gaacaacaac atgtgatgat ttttcccatg gatagtcaac
    54601 gcctaattta cgagagtaga aatcgagtat ttcgggcacg tttccaaaaa tatcttttgc
    54661 atacatttca tattccgggt caacatagta gttgacttca atgttgcgcc aacggtcttt
    54721 aaccacttta aaattgctaa cagccatcat cgtcagatag ggtgcagcag gcaatgactg
    54781 tttccatgta tctgtacgtg taccgtcagc atttttaact gaagaagtca tcaagccgtt
    54841 gctaagtgtt acaaatgagc tgtcaacagt tatgctgatt tcctgagtca tgttttgatt
    54901 tggagaatca atagtaggga accaaacgga gttgctttgt gtttcgcctt gtgtccaaac
    54961 ctgtcgtggt ttattgggat tttttccatc ggcatttaca aagtaaagac ctttatcact
    55021 attaattgct acactgccac caatgttttt tagttcatca ggctttgata tatagtcaat
    55081 ataaactgtg taggtttcag ttctgttgta aaagcggtcg aggttgatgg ttaatgaatc
    55141 gttttggtag gagtaggtta gcttttttct tccggtgtcg gtaatcaggc tgacttcgtt
    55201 gaagtccatg ccacgtgcat ttagtatcag cgtgtttaca ctggtaaaat agggtttagc
    55261 ggttacttcg gcaatgccgt acaggtactt tttattccag tcgaaacgta cttttagttt
    55321 cgtatgttgc aagtcgttta agatggtgtt gctggcgcga tagtcttcgt agggaggttt
    55381 tacttctatg gcaggaaggt catgtgtttc aacaggagtt ttttgagttg ttttacatga
    55441 tgataaaagt gcaatagaga tgattgcagc aaagagagct tttgtaaaat tcatgttcag
    55501 gaaaatttga aggcgtgaaa taatgaaaat tatgctgcta aattggttaa aatcaagtta
    55561 aggagcaaaa agtgatacaa agggggtgct tcacatcttg acgagagact tttttgattt
    55621 cacgcaaaga gcgcaaggat gaaattcact tagcggcctc tgcgtgttta tttctttgcg
    55681 tcttggcgag agaccttttt tttcacgcaa agctcgcaaa ggggtatttg tagtaagtct
    55741 cgttttcttt tcacgcaaag gagatatatt tcttagcggc ctctgcgtgt tttatttctt
    55801 tgtgtcttgg tgagaataat tttgttttca tgtaatgatg tttttaaaac ctaaagttta
    55861 tcaataattt tcatgacacg gtctgtcata gcattacagt aaacagcatt aaactcaaac
    55921 aactcttctg cagcatacga tttttctatt tctgcacgaa gcatagcttt agaacggttg
    55981 agtcgtgttt tcacattggc ctcgctgatg tttaagagtc cggcagtttc tgatacgttc
    56041 atgccattga tttcgcgaag ggagaaaacc attctgtatt catcaggaat tttgcttagt
    56101 gcttcttcta taatatctcc caactcacga ttttgtatta atttttgagt atcgttattt
    56161 gttcctgtaa acattggttg tgaattttcg tgtacatctt gtttgatttc atttttaaaa
    56221 cctgattttt cttttttacg gaaacagtta ttcaacatta ttcggataat ccaggtttta
    56281 aaattcgatc gcccttcaaa ttgtgaaaga tttctatatg catctacaaa agtatcctgc
    56341 ataagatctt ctgtgtcttc gtgattgtaa ttataagaac gaccaatttt ataaagagta
    56401 ggattaaatc gccttactat aatttcgtaa agcgattttt ctccgtttaa tatacgttcc
    56461 gcaatttgtg tttcggataa ttctttataa agttccatat tattatttta caaatttagc
    56521 tagttgtgcc attgaaaagg cagatattgc cattaccaaa tcacgaacgg caacatctac
    56581 atagttgaaa cccataagga gtgttaaagc aattaatgta agccatgcag ctactatctg
    56641 accaccaatt ataggttttt ttaagacaac aattcctgct acgatttcaa ttacaccaac
    56701 aagcatcata aatgctgcag gagttagcgg aagcataccg actattgttg gattaatata
    56761 ttgtggccaa ttggtaagaa ggtttgtaaa tttatctgca ccggcaacga taggaacaac
    56821 tacaaaagtg tatttaagta agttgaatgt ttgggatatg attttatttt ccataatttt
    56881 tttgaatttg aagttaattt aaaatttctc cggaaattga agtgctattc ctttggcaag
    56941 gtcgtcagcc atgtcgagca tgtgttggtt ggcctcatca tagtgagtga ttgcattgtt
    57001 gtaatcttta ttcaataaat caacagcgta tgttgtagtt tggtcaatgt gcatttccat
    57061 catgtgttgc atatcgtgcg gtggccagtt tttaggatta gcagttgaaa gaaagtttgc
    57121 aatatcgtta gcattggttt tccagtttgc taatgcaagg ttaagggcgg gctgatcgtt
    57181 attttttgcg gcttcgagta caggcacagc taagtttata tgtgttgtga gcaaagaaga
    57241 aagtgtgtca ccggcagcct gaccatagta aggaactatt gcagcgccaa tatctttttg
    57301 attttgtaat aaacgattta aagaagaaga taatccggaa ggattgttgt agtaggcatc
    57361 aacagttgca taagtgtatt tcatgtggtc gttccagagt ttgcgcaagc catcttgcaa
    57421 gttgtaatat tgtgtacttt ctaatggatt agagtcgtca tctttcttgc aagatgtaat
    57481 taaagtggca agagaaaata agagtagaaa tacagagagc gatttcaaat tcaactttct
    57541 tttggttata cttgaagttt cagcaggttc aagagaattg atgtgatgta atgatgtgtt
    57601 tttcattttt attatttttt taatgtttat actctattag agtaaacgaa aaaacaaaag
    57661 gttacaaaaa atctaaaatt gtattaaagt tggactttat gtgttttttc tttgcgtctt
    57721 ggcgagaggc tttttcattt cacgcaaagc tctcaaagtg atatttgtag caagtgtaat
    57781 tttcttttca cgcaaagagc gcgatgggtt atcatttagc tttcttttgt tagtttaatt
    57841 aaagatgtac attgaaaaat ggcatccacc taaatagttt tcgattatta cctatataat
    57901 tcccaactgc aaaacttaat gtatagatga cgaagatgtt agggcttttt ttaacgatgg
    57961 ttgggcaaaa gccatattct gatagctacc agaatgtgtt tattagtaga atgatatgtg
    58021 ggcacaagtc ataagacttg cgccatgtgt ggcttttttc atttcacgca aagagcacaa
    58081 aggttttttc tcttagcggt ctctgcgtga tttttttctt tgcgtcttgg cgagagactt
    58141 ttatttcacg caaagctcgc aaaggggtat ttgtagcaag tgtagttttc ttttcacgca
    58201 aagagcgcag agagtgaatt ttcttagcgg cctctgcgtg tttttttctt tgtgtcttag
    58261 cgagcaatct ttttttcacg caaagctcgc aagggagata tatttcttag cggtctctgc
    58321 gtgatttttt tctttgcgtc ttggcgagaa accttttttc tttcacgaaa agctcgcaaa
    58381 gggataataa ttttaaatag tctgtgaatg ttaaagcaga aatgatagaa tgtaaactat
    58441 taaataattt gcttaaatat aatatcctaa tatttacaaa ttattaacga ttctatgaat
    58501 gccttctttt agatacttag tgttaaaatt gattaagaga gctaattttt tcccagacaa
    58561 ttttaaatat gtaagcgttt gtgcaaaatg aactggggcg agcgtttcta cagattttat
    58621 ctcaacaata actttatcat tgattattaa atctaatctg tatccaacct cttgttttac
    58681 atttttataa atcaatggca ttggaacctg gacttttaca tcaaaaccag cctgaattaa
    58741 gtcataagtt aaagcagatt cataagcaga ttctaataag cctgggccta agactctatg
    58801 catttctact gcaattccaa ttatttgata tgagagttca ttttcaattg aagtattcat
    58861 ttcggtttat aattgtttca aatcgaaaga tacaaaaaat agttttgtta aataaaaatg
    58921 tttattcaaa atatagtgtg tttgtttttc acgcaaagct cgcaaggaaa atatatttct
    58981 tggcggtctc tgcgcgtttt tttctttgcg tcttggcgag gaactttttt tatttcacgc
    59041 aaagctcgca aagtggtatt tgtagcaagt gcagttttct tttcacgcaa agagcgcaaa
    59101 ggagatgtat ttcttagcgg tctctgcgtg atttttttct ttgcgtctta gcgagaaacc
    59161 tttttctttt tacgcaaagc tcgcaaagtg gtattagtag taagtattgt ttttcatttc
    59221 acgcaaagag cgcaagaggt atccgtataa ttttctttgg tggttttaat taaagatgta
    59281 cattgaaaaa tggcatccat ctaaatagtt ttcgattatt accagcataa ttaataacac
    59341 ccaattgtaa accatgtaat tcttttgtgt gattgaacaa agctattgat aatccttttt
    59401 gtctgtcaga actacaatat ccccctatat aaaatccatt cattttacct gcatctgctc
    59461 caaaaataga cagcccgact ccattcaata tcctttcctc atgttttctg tcagaagaaa
    59521 gaaatagccc aacacctaaa ccattcaaag tatcaggtac tactccaaat gcagaaatac
    59581 caacaccatt taacttactt ccatactgtg aaattgaagc gaatgaaaca ccgttaaatt
    59641 gataatattc agcacgtatt actccgattt gtactccgtt tcccgaattg ccatcggaat
    59701 acataaaaat atttattcca ttaaaagggc tacttgcggt atcggtatta ttaaaacgat
    59761 aaccattata attacaccag ttaccaaaac agattccaca acgtgttgtt ccaaacaagg
    59821 gatacttatt cgcatatata tcaatttgcc cgctacaaaa atttgtggca aatagaaata
    59881 ttagtaagaa aactgatttg tgaaatgtca tttaggatta ttttaaattt taaaataaag
    59941 tagatttaac tattcaacat aacttttttc aaacaccggt caatcatttc aacagcttcg
    60001 tcaacagttt ctttttttgt tctgaacgct aaaatggcca ttcgtattac aaaccgatta
    60061 ctaatgattg acgaacttaa gaatacagct ccatctttat gcatttcttc catcagcagt
    60121 ttattctttt catcagcatc agtagcaaaa ggataccaaa agtaacttac cgataaatca
    60181 ggttcagggc cgagacaaaa accctgctcc gccaatttca ttctgaaata ttgtaccaat
    60241 aaaagtttct cttcaagaca agcaataaaa ggttctatgc catgatattt taatggcagc
    60301 cacactctaa gaccgcgaaa atgtttggtg agctccggtg ataggtttgc cggactttta
    60361 agcaactctt cattttgtcc gtcctgcatg tagttggcgg tgtaataatg tgagtgtaaa
    60421 actgcattgc catctttcac taaaactgca ccaacaccat agggtagaaa caaaccttta
    60481 tgcgggtcaa caataatgga gtcggcacgt tctattcctt taaataaatt tttctttgat
    60541 gtgagaataa aaaatcctcc ataggcagca tctacatgaa accaaagttt ttgctgagca
    60601 gctatatcgg caatatcatt taatgggtca acagtaccgg tatctgttgt tcctgcagtg
    60661 gcaacaatta aaaagggatg gagtccttct gctttatctt tttctattaa tgcaattaat
    60721 gcgtcaggtt taattttatg acaatcatcc aaagggatgt aacgtacaat aacatcttca
    60781 agtccaatta tccgtaaagc tttttgtgtg gaatgatgaa cctgctcact caggtaaact
    60841 acactttttg gaacaacttc gtttttgatt ttaaatttat ctcttgcagc agtaaacgca
    60901 attaaagtag aaacagagcc acctgatgat aaacacccaa ctgccctttc aggaaaagaa
    60961 aaaacgtttt tcaaccaatt aacaacttcg ttttctatag tagctgcacc gggagcagca
    61021 aaatatgcac cggcaaaagg attggtgaca gcagcaataa aatcagctaa ggctgcggtg
    61081 taaactcctc ctccgggaat gtagcctaaa tgttttggcg aggctgcatt aattccactc
    61141 tctgcaactt ctttctgata tagattcagt atatcatgta aagcagcagg cttattattg
    61201 atagataaat caccaacggt tttttcctga taacctttta tggtatgtag tgttgaaata
    61261 aagttgttgg cataatcagc agtttgatgt aacaattcag ttctttggga ttcgggtaaa
    61321 tccaattgct tgctgattaa attaagtttg tttaattggt caatgatagc ttcctgactc
    61381 atcttaaaaa tagagtaaag taatcaattt attatttgtg tttgttggtt taaagaatgc
    61441 aaattagtct aatgcagttc ttgatgtaat tgcaattctg ttccatccgt taattgtaac
    61501 aatggtcatc attactgccg ccatttcttt atcggttaaa tgttttttgg catcatcaaa
    61561 aacagtatcg ctaacgagat tgtttgcaat taatgtcatg gcttctgtaa gagctaaaac
    61621 tgcacgctct ttttctgtaa acaaagtagt ctctcgccag gcgtccaata aaaataatcg
    61681 ttgtgatgtt tcaccaattt tcatggcatc gcgaacatgc atgttaatac aataggcaca
    61741 accgttgatt tgtgaggcgc gggtttttat cagttcaaaa agtattttat ctaatccgct
    61801 ttctgcaaga tatttttcaa gatttagcat agcatcaaat gctttagggt cggcttttga
    61861 taagttgaat cgttctttca ttttaatatt tattttaact gtaaaaatat aattaaagct
    61921 taacaaattt ttttacaaaa ttgttttcat tattgtctct aacataaata gaataaatac
    61981 cctgtttaaa atcacttata tcaaaatttt cgaatcgctt ggctgccttt tcaacttctt
    62041 tgcgaatttt
//
LOCUS       DAOOOI010000007        56439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_76_length_56439_cov_25.590522, whole genome shotgun sequence.
ACCESSION   DAOOOI010000007 DAOOOI010000000
VERSION     DAOOOI010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 56439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56439
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76_length_56439_cov_25.590522"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(381..1316)
                     /locus_tag="PKX59_02360"
     CDS             complement(381..1316)
                     /locus_tag="PKX59_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005651602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HOZ89739.1"
                     /translation="MSKLDEAKKILKELGLPTAQQNEISAYTLLALCGIKPRNSWSKA
                     TRKSLKVTKGIMAFVLDVHKKEYAPNTRETFRRQVLHQFVQARIADYNPDNPKLPVNS
                     PNAHYAITNEALEAIQNFGTKDWKKAVEKFKSEAGELAKRYKKERKQNLIPVKLSNGK
                     TIKLSSGKHNEVQAAIVHNFAARFANGGSILYLGDTAKKDLFVDEKKLKELGIPIDQH
                     SKLPDVVIYDEKKNWLFLIEAVTSHGPVSPKRIVELEEFLKGCKAGKIYVTAFPDFTE
                     FKKHSNNIAWETEVWLADTPDHMIHFNGDRFMGPR"
     gene            complement(1313..2899)
                     /locus_tag="PKX59_02365"
     CDS             complement(1313..2899)
                     /locus_tag="PKX59_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOZ89740.1"
                     /translation="MADKKLIEKIADELPSHCADRLGVNYAQSVNQEHKKTNGQFFTP
                     IEIAGLMGTFAEVSESSLRILDPGCGTAILTCALIESIVQNNSKLQEIDLIVYETDKA
                     LIPYTKESLDYLRSWLNAKNIELKFSIHTDDFILENAGCLTDNGNLFSQTINPFDIIV
                     SNPPYFKLPIDDKRAIAAKAVVNGHPNIYAIFMAISARLLKENGQLIFITPRSYASGS
                     YFKLFREYFFKIIEIDNVHLFVSRKDTFNRDKVLQETVIIKGTRKEKTNPKHKVHVYS
                     SHGLKDIDSPIIKSFAQKELIDLSSNEKILYLPTSDSDEAILNVFKNWSGNLNKYNIQ
                     ISTGPVVAFRSKDHIHDTYQNGTVLLAPLFWLHNVQQMALDWPMPKPEKGQYVRVHDE
                     SKSILIPNKNYVLLRRFSAKDDKSRLVAAPYFCNFIEAEYIGVENKVNYIYRPKGHLE
                     RNEVIGLCALLNSSLFDSYFRIFNGNVNVSATELREIPLPPLETIKEIGNSVILSNDF
                     SVDKANKIVNEQFEFTLATI"
     gene            complement(2889..3197)
                     /locus_tag="PKX59_02370"
     CDS             complement(2889..3197)
                     /locus_tag="PKX59_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOZ89741.1"
                     /translation="MKNIAEIIRELREQNGLLLRQVAAEIEIDQALLSKIERGERMPT
                     KDQVIRLAKFYKVDLNEFLIAFISDKLVYELQNEEVALKAMQVAEKKINYIAKKKNGR
                     "
     gene            complement(3575..4114)
                     /locus_tag="PKX59_02375"
     CDS             complement(3575..4114)
                     /locus_tag="PKX59_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOZ89742.1"
                     /translation="MTTLTKTTWGIDQAHSEITFKVKHLMISNVKGNFAKFEGTVQSE
                     DSNFDNANINFSMDASSITTGDTQRDTHLKSGDFFDAEKHPSISFKSENFSKTGDDTY
                     ALNGQLTMKGISKPVKLIAEFGGMMKDPWGQEKAGFTLSGKINRKDWGLNWNTALETG
                     GVLVSEEVKIAAEIQLVKK"
     gene            complement(4148..4870)
                     /locus_tag="PKX59_02380"
     CDS             complement(4148..4870)
                     /locus_tag="PKX59_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOZ89743.1"
                     /translation="MENKVIHRAENRGNADHGWLKAKHSFSFASYYNPNQMHFGVLRV
                     LNDDHVEPGMGFGTHPHDNMEIITIPLAGALEHKDNMNNHGIIKYGEVQVMSAGSGVT
                     HSEFNASKTDAVKLFQIWLYPDKENVKPRYDQLSLNVNDRKNKLQTIVSPDTEKEGLW
                     IHQQAWLSMGNFDKNKSTSYKIKKEGNGVYAMVVKGEFEINHNLLHERDAIGLWNTSE
                     INFIAKTEDAEILLMDIPMQLN"
     gene            complement(4984..5586)
                     /locus_tag="PKX59_02385"
     CDS             complement(4984..5586)
                     /locus_tag="PKX59_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOZ89744.1"
                     /translation="MAKTDSNKPFNLILKNISRYISLSEEEEAIFTSLLSARIVRRKQ
                     FVMQAGEVFTASCFVNSGCLRAYNIDKSGTEHILQFAPPGWWIGDFYSSITGRPGILF
                     IDAIDESEIVVLTKENRDLLFDKIPKFERFFRILIENSMASQQERILDKLSLSAEERF
                     EKFCLKYPALVSCLPQKYIASYIGVTPEFFSKMRARMTKN"
     gene            complement(5570..6148)
                     /gene="lpcA"
                     /locus_tag="PKX59_02390"
     CDS             complement(5570..6148)
                     /gene="lpcA"
                     /locus_tag="PKX59_02390"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019934331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HOZ89745.1"
                     /translation="MSEIIRQHFEEAKKVLETFLSVDKNIAAVTKAGELMTASLAAGG
                     KIISCGNGGSMCDAMHFAEELAGRYRNDRKPIAAISISDPSYMSCVGNDYGFNYVFSR
                     FVEGVGNKGDVLLAISTSGNSANVLEAAKVAQAKNIIVVALTGKDGGALKNHCDVEIR
                     VPHSGFADRTQEIHIKIIHALIDYIEQHGKNR"
     gene            6282..7862
                     /gene="lnt"
                     /locus_tag="PKX59_02395"
     CDS             6282..7862
                     /gene="lnt"
                     /locus_tag="PKX59_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOZ89746.1"
                     /translation="MQVKKKLILSLLSAVMLSLGWPERGLTPLIFIAWVPLLLLEEYS
                     HQKKEQKKFGLFGWTYLTMLGWNALTTWWVCNASFGGGLFAIFCNSLFMTCVFYAFHI
                     VKKRKGHVWGYTALIIFWVAFEYLHLNWELSWPWLTLGNVFATKPQWIQWYEYTGVLG
                     GTVWVLLVNILITRIFVASADKLKRSVLYATVAIAFPGLISLLRYYTYKETENPVNVI
                     ATQPNIDPYGEKFSGMSNEEQLIPVLRLANLELNDSSDVILAPETAIANSVWEDELEL
                     YPEVKLIRKYLQVNPRATLIIGASTNRAYLNGQKPSSTARKFKDQDGYYDSYNTALLF
                     EDGKPAQIFHKSKLVPGVEKMPYPKIFGFLEKLSIDMGGTAGSLGIQDEPTVFTTKDK
                     MIIAPVICYESIYGGYLAKYVRKGAQWIAIITNDGWWGNTPGYRQHFNYARLQAVSLR
                     RSIGRSANTGISGFINQRGDAFQQTPYWHDAVVRQTINKNDKLTFFAQYGDYPGLIAS
                     ILVLPVIVITFFGKRKNQ"
     gene            7900..9303
                     /gene="lpdA"
                     /locus_tag="PKX59_02400"
     CDS             7900..9303
                     /gene="lpdA"
                     /locus_tag="PKX59_02400"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOZ89747.1"
                     /translation="MENFDVAIIGSGPGGYVAAIRCAQLGFKTALVEKYDTLGGTCLN
                     VGCIPSKALLDSSEHYFNAVNKFSEHGIKIKDIKADMEQMIKRKAQVVKQTCDGISFL
                     MKKNKVTVLTGHATFVDSKQLDVVDAGGKSNRVGATNFIIATGSKPSSLPGIEIDKKR
                     IITSTEALELKTIPGHLVVIGGGVIGLELGSVYARLGAKVTVIEYMNAIIPTMDRALG
                     KELQKVLSKEGFTFLLGHKVQSVKSSAKQVEVVGESSSGEKVNIIGDYCLVSVGRKPY
                     TEKLGLDKAGVALDERGRIITDEKLQTNVPGIYAIGDVIKGAMLAHKAEEEGVFVAEQ
                     LAGQQPHINYRLIPGVVYTWPEVASVGYTEEELKESGVKYKTGNFPFKASGRARASMD
                     TDGFVKVLADSTTDEILGVHIIGPRAADMIAEAVVAMEYRASAEDIARICHAHPTFTE
                     SFKEACLAATANRAIHI"
     gene            9333..9899
                     /locus_tag="PKX59_02405"
     CDS             9333..9899
                     /locus_tag="PKX59_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOZ89748.1"
                     /translation="MNIQIKEIITVSMILFSVIDIIGSVPVIIGIRKSVGTIHPEKTS
                     LVALMIMIVFLFVGENILSLIGLDVSSFAIAGSFILFFLALEMILGVKLYKDEMPASA
                     SIVPIAFPLIAGSGTMTTLLSLRSQYDMLTIIIGVLLNMIWVYLCLKNLGFIERLLGP
                     TGIAIVRKVFGFILLAIAVKLFRSNLGI"
     gene            9904..10530
                     /locus_tag="PKX59_02410"
     CDS             9904..10530
                     /locus_tag="PKX59_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HOZ89749.1"
                     /translation="MAKKKFYVVWKGVMPGVYHSWDDCKRQIDGFAGALYKSFETENA
                     ALTAFQKGLSGFQKKSESQHLFQVEKSSSKPIKESIAVDGAWNTKTLESEYRGVDVKT
                     GKQLFLKGPYADGTNNIMEFLAIVHALAYCKRHHINLPIYSDSMTALKWVKTKKANTK
                     LASTGRNEELFELIDRAEEWLKNNIYANKLLKWETQIWGENPADFGRK"
     gene            10733..10918
                     /gene="ccoS"
                     /locus_tag="PKX59_02415"
     CDS             10733..10918
                     /gene="ccoS"
                     /locus_tag="PKX59_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HOZ89750.1"
                     /translation="MSVLIILIGASLVVAAGFLVSFFWAVRSGQYDDDYSPSVRILFD
                     NDKTLTSDKSNSNITDN"
     gene            10923..13049
                     /gene="ccoN"
                     /locus_tag="PKX59_02420"
     CDS             10923..13049
                     /gene="ccoN"
                     /locus_tag="PKX59_02420"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HOZ89751.1"
                     /translation="MTIEKFRYDNKIVAMFAWATVIWAIVGMLVGLLAAFQLPFPALN
                     FDAHTTFGRIRPLHTNAIIFAFVGNGMFMGIYYSLQRLLKARMYSDVLSYIHFWGWQL
                     IIVGAAITLPLGITASKEYAELEWPLDIAIAIVWVIFGINMFGTLVRRRERHLYVAIW
                     FYIATVVTVAVLHIVNSLAIPVTLFKSYSLYAGVQDALVQWWYGHNAVAFFLTTPYLG
                     LMYYFLPKAANRPVYSYRLSIIHFWSLIFLYIWAGPHHLLYTALPDWAQSLGVVFSIM
                     LLAPSWGGMINGLLTLRGAWDRVRDNPELKFYVVAVTAYGMATFEGPMLSLKSVNAIS
                     HFTDWTIAHVHIGALGWNGFLTFGILYWLIPKLFNTSLYSKKLAGIHFWLGTLGIVFY
                     AIPLYWAGITQHFMWKEFTEDGVLRYPNFLETVVQVVPMYKLRALGGSLYLLGSLVMV
                     YNLIKTARAGKMVANEDAEAPALEKNYVAHDDHWHRWIERRPAQMLIGSLIVILIGGM
                     VEIIPTFMVKSNVPTIASVKPYTPLELQGRDIYVREGCYTCHSQMVRPFRYETERYGE
                     YSKPGEFVYDHPFQWGSKRTGPDLHREGGKYPNSWHYNHMLDPQTMSPGSIMPSYAFM
                     IDDDLDTTTTAAKIRAMQKLGVPYPEGYDAQANKDLMKQANSIASNLEKDGIKVMGNK
                     EIVALIAYLQRLGVDIKAEKTTASVN"
     gene            13243..14421
                     /locus_tag="PKX59_02425"
     CDS             13243..14421
                     /locus_tag="PKX59_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOZ89752.1"
                     /translation="MKKIINLICLIMFISLSAWAGDTQATETTKVAAAQPPDWLMNAN
                     TYLIAFVVIILLATILALYRSSLKLLKKITSFCFIMFVSGSAFAAEEAAPAEATKVTA
                     IQPPDWLLHTNTYLIAFIVFILVVTIVVLFKTNMKLIRMIAPHAFDDEKQTAEAGAVS
                     GVKNRETFMRKVYLRLVDSVPVAKEQDILLDHDYDGIQELDNNLPPWWKWGFYITIIA
                     AVIYMLAYHVTGTGKLQAEEYADELAVAAKQKEERMKNSAENITEENVTVLVDAAEIA
                     KGKDIYEKNCSACHMADGGGMVGPNLTDSYWLHGGGVKNIFKTITYGVPAKGMISWQA
                     QLSPKQIQEVTSFIMTLHGTKPAAPKEPQGDEWKDEPAAGATATDSAAVATTAEAVKK
                     "
     gene            14439..15842
                     /gene="ccoG"
                     /locus_tag="PKX59_02430"
     CDS             14439..15842
                     /gene="ccoG"
                     /locus_tag="PKX59_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HOZ89753.1"
                     /translation="MVENKMEHIDESFRDSIATVTEDGKRAWIYPKKPKGRYYNARTW
                     VSIVFLIIFFALPLIKINGHPFFLLNVVELKFVLFGIAFFPQDFYLFVLAMLTFVVFI
                     ALFTALFGRLWCGWTCPQTVFMEMVFRKIEYLIEGDFMKQKALDKRAWDAEKIRIKTT
                     KHLVFFFIAFLISNTFLMYIIGFDEWKKIITDNPFDHIGGLFATVIFAGVFYGVYARF
                     REQVCIVVCPYGRLQGVMLDPNTVVIAYDYKRGEPRGKLSKDNEEHGDCIDCNQCVQV
                     CPTGIDIRNGTQLECVNCTACMDACDDIMDKIQKPRGLIRYTSENAIKNNAKFKINSR
                     IIGYCVVLLVLFSALVVLLARRSNIETTILRASGLLYQKPDESHISNLYNIALINKTF
                     GNMPIELKIVEPKGEIKWVGNGITHLKEQSVAQSTFFLILPKSEIKKTKTKVVFEIWS
                     EGEKIDRYETSFLGPNN"
     gene            15848..16285
                     /locus_tag="PKX59_02435"
     CDS             15848..16285
                     /locus_tag="PKX59_02435"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HOZ89754.1"
                     /translation="MKFNWGHGIALVLIIFASSILVFVYVASKQKNELVTDKYYEKAV
                     AYQQRIDAEKNALNENADVKYNINSGQLMLTLGRNIPNANGTIQFYKPDDATKDFNTS
                     FQVNDTGFYSATIPQLTHGQWKVNVSWNVDSKEYFYEKKIFIP"
     gene            16285..17013
                     /locus_tag="PKX59_02440"
     CDS             16285..17013
                     /locus_tag="PKX59_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOZ89755.1"
                     /translation="MNEYTFYYAALLLGFLGSFHCVGMCGPIALMLPANKLDNRRAAF
                     FGRLFYNIGRMITYMFIGMLFGLLGAAFVLNGFQKQLSIITGIILLLVAIFTLIPSTR
                     KKVLSVSSKMNIPFRGVLRKLFSGKTALHHFGIGVINGFLPCGFVYVATAGAINSGSV
                     LNSVLYMALFGLGTFPIMLLVSYAASWCSFSFSRYVTKATPWISAALALFLIYRGAFM
                     NINADCHKHSHPPTMVECSKPETL"
     gene            17105..20128
                     /locus_tag="PKX59_02445"
     CDS             17105..20128
                     /locus_tag="PKX59_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ89756.1"
                     /translation="MSHKLLDALMHLFAILANAEKFSLEGRSIVESFLRQQISRAYVL
                     KYLGLFDEYLENIRGKATEGKLKKRLSVNSVKVLRICTDINAELDTRQKHIVLMRLIE
                     FVFTSEKIIDSQEKEFLYTVAEVFHIAPDDVQLCIELACNNAEEKYSDTPKYLVLSSQ
                     DDNSSLNTKTQIISGLNGVFRFLFLKNAGLLFVRYHGDESFTLNGLPLNDDLCYVFSA
                     GSVIRGSKVNTIYYTDIIRLFSNFKQQSLLHFDVQHVYYDFSNGKRGLNDVAFSATSG
                     NLVGIMGGSGAGKSTLLNILNGNNRPTQGKVTVNGIDLHRESHLLEGMIGYVPQDDLL
                     IEELTVYQNLFYNAKLCFGNLTDSEIDERVKALLLNVGLSEASHLRVGNALSTSISGG
                     QRKRLNIALELIRAPEVLFVDEPTSGLSSLDSENVMDLLKQLANHGKLVFVVIHQPSS
                     DIFKMFDKLFVLDIGGYPAYFGNPVDSLLYFKQRASFADADYSECGTCGNINPEQIFS
                     ILESKVMDEFGFPTQQRKLTPADWYQLFKEHNSNSSKSNQRQPVQGSANQKPGWLQQL
                     KVYMTRDMLSKFKNKQYMLITFLEAPVLAIILAWFLRYFNPDGSYVFSENKNLVAFLF
                     MAVIVAIFMGLTVSAEEIIRDRKMLKREKFLNLSRSSYLYSKMMVMFLISAIQTFSFV
                     IIGNLIFGIQSMTFDYWIMLFSVSCFANMLGLNVSSMFDSAVTIYILIPFLIIPQIIL
                     SGVMVKFEDLNPHLTSQRHVPLIGELMASRWAFEALAVNQFSNNPYQKYFFNVDRQMS
                     EATYKKDFWLQKLNDIAEGVSKNKSKNPKVDVELITNELHKEALVNKNFKTAIDFTKA
                     NASEIASQIKPLTDILRNYYIEKYNNASAAKDSFVKKELKHSSVAMLNKQKEKYTNNA
                     LNDLVLNKNDFTILVQDGNSIVQRFQPVYMQPDPTTWFRSPLYVYEKNFFGMKIHTWY
                     VNLFIIWLMSFILMTTLYFDSFRKLIHWVGHSSKR"
     gene            complement(20272..22830)
                     /locus_tag="PKX59_02450"
     CDS             complement(20272..22830)
                     /locus_tag="PKX59_02450"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOZ89757.1"
                     /translation="MLIETITTESQMTTYEYDSVLKASLEYFEGDELAATTWMNKYAM
                     KNKEGKFVERTPHDMHLRMAREFARVEAKYNLLNDGSRTSHLSEYGKKREQLTTEKIT
                     ALFDRFRYVIPQGSVMSALGNPFMIASLSNCIVLPELHDSYGGIFFADQQLAQLYKRR
                     CGAGLDLSTIRPEGMRVSNAAGTTTGAVSFMERFSNTTREVAQLGRRGALMLTLDIAH
                     PDVEKFITVKQDLSKVTGANISVKLSDEFMVAVNENTDFNLRFPVDSTDPYIQKKVNA
                     RELWKTIIACAHRTAEPGIIFWDRQHHYSTSSVYPGFKNVSTNPCSEIAMQGGDSCRL
                     IAINLLSFVDHPFTQKAKFNFTRFYEVTYESQRLMDGLVDLELEAIERILQKIESDPE
                     PDYIKEVEMRTWITLYEAGKKGRRTGLGFTALADALAAMNIAFDSDNALSIVEMMMKE
                     KCRAEFDSSIDMAIERGTFEGFDTAIEDQSEFVNMMKAELPDIYNRMMKYGRRNISIS
                     TVAPTGTLSLLSQTSSGIEPVFMLSYKRRRKVNAEDSKAKTSFVDQSGDAWQEFEVYH
                     HGLKKWMTANNSTDYSKSPYAGSTANEIDWKKRIKMQAVVQKYTTHSISSTINLPENV
                     SAETVGEIYMQAWSAGLKGITVYREGSRSGVLVKKEEKKAEPVITESSAPKRPEILAA
                     KVIRFMNADEKWIAYIGILDNKPYEIFTGKAEDAFSIPAWVSSGWIIKSKNGHGKSRY
                     DFQFNDKEGFKVTIEGLSRSFDKEFWNYAKLISGVLRHGMPIPQVVNLVENLHLYSDS
                     INTWKNGVMRALKQFIPDGTKVNDKVCAECGDKEGLYFEEGCIKCKSCGHSKCG"
     gene            23109..24485
                     /locus_tag="PKX59_02455"
     CDS             23109..24485
                     /locus_tag="PKX59_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOZ89758.1"
                     /translation="MKFKTIIEPFRIKMVEPIHLSTEEQRVGYLKQAHYNPFLLNSNQ
                     VLIDFLTDSGTSAMSSDQWSAMMNGDESYAGASSWLRFEKEVQSLTGMPFVLPTHQGR
                     AAERILYGKLGGKGKTFISNTHFDTTRANIEFSGAEAIDIPVKEGLDFASDFPFKGNM
                     DIVLLEKTIAEKGAANIGAVILTVTNNSGGGQPVSMENAKAVADICRKNKVLFVIDCC
                     RVAENSYYIHHNEAGYENKTYRQIAEEMMCLGDAAVMSAKKDALVNMGGFLAVKDADL
                     AESCKQLLIITEGFATYGGLAGRDMEAIAVGLQEVFDPGYLNYRIVSTRYLGENIKSK
                     GVNIIYPVGGHAVYVDAKSLYPHIPPHEYPGQALVCELYRIGGLRSVEIGSVMFGKYD
                     SNGKLIPAPMELVRLAVPRRVYTQSHIDYVIEIFDEIVRTKDKVKGLKIVKEPQFLRH
                     FTAHFEPL"
     gene            complement(24561..25385)
                     /locus_tag="PKX59_02460"
     CDS             complement(24561..25385)
                     /locus_tag="PKX59_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOZ89759.1"
                     /translation="MKWFLAIKKQVRPLILWMLLLLQIATIGNAQQIPPQSNPPRLVN
                     DYAGVLNDADENLLEQKLVQFNDSTSTQIAIVLIRSTGEYPVDDYAIALFRDWKIGQK
                     NKDNGVLILAAMEDRRMTIITGYGVEGALPDAICKRIIELTLKPAFKSQQYYNGLDAA
                     VTEIMQRTKGEYVSDGKNAKNNSPAWAPILFVFLIFGVVMLMKVFSVRRYAQLNNLSF
                     WTAWMLLNAASNRRNSSGGGGVWGGGGSSWGGGSSGGGFGGFGGGSTGGGGATGGW"
     gene            complement(25363..25803)
                     /locus_tag="PKX59_02465"
     CDS             complement(25363..25803)
                     /locus_tag="PKX59_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOZ89760.1"
                     /translation="MANAADYFSDEQQLLLKNAIARAEKHTSGEIRIFIDSKVKSEII
                     DRAAEVFENLKMHETKLHNGVLIYVAIENRQFAIIGDSGIHEYVSQVFWDKVKEEMIP
                     FFKRKDYTGGLLHAIEETGQKLKKYFPVAKNDSDELSNEVVFSD"
     gene            complement(25806..26396)
                     /locus_tag="PKX59_02470"
     CDS             complement(25806..26396)
                     /locus_tag="PKX59_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOZ89761.1"
                     /translation="MKRSVVIIGILLVVAFIIYRLFAGTYNSMVTADENVKGKWAQVE
                     NQYQQRMDLIPNLVNTVKGAAEFEKGTLTAVVEARASATQVKVDPDKLTPENIQKFQE
                     AQGQLSTALGRLLMVTENYPVLKANQNFLELQATLEGTERRIAVARKDFNEAAQQFNT
                     YIRKFPQTLLAGMFGFSPKGYFQSDKGANKAPEVKF"
     gene            26576..26845
                     /locus_tag="PKX59_02475"
     CDS             26576..26845
                     /locus_tag="PKX59_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOZ89762.1"
                     /translation="MIKTVAIEITGKVQGVFFRKFTQQTGRRLNLCGFVRNRSDGSVY
                     VEATGSELQLNELVAWCHKGSPMSLVRQVVVREISVIHNEPFQIL"
     gene            26887..>27221
                     /locus_tag="PKX59_02480"
     CDS             26887..>27221
                     /locus_tag="PKX59_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019866759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOZ89763.1"
                     /translation="MSGIKKLAGQTVLYGLSSIVGRMLNYLLVPIYTRIFSPAEYGVV
                     SELYSYSAFLLILFTYGMETAFFNFMQKEENKNKVYENCISLVSYTTTILVLLMIFFS
                     SPLAATIEYP"
     assembly_gap    27222..27321
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27322..28248
                     /locus_tag="PKX59_02485"
     CDS             <27322..28248
                     /locus_tag="PKX59_02485"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOZ89764.1"
                     /translation="SNVIASGFTMLLLYRQFSNLTWHLDSALIKRMIYYAFPLLIAGF
                     AGMVNETFDRAVYKYLAPDANHALRDLGIYSACYKLSIIMTLFVQTFRYAAEPFFFAQ
                     HDKEDKKETYAKVMTWFVITCSFIFLGVMLFIDVIKLFIGEQFRSGIDVVPVLLLANL
                     CLGVFYNLSMWYKLTEKTRYGAYFSIFGAVLTIILLFGLIPSFGYMGAAWATFITYFA
                     MMIVSFITGQKHYPIPYKVKNDVFYVLLSLMLFSLSAYLQHQLDLPGSVKWIVNIILL
                     FVFPFVVFRMNKDLLVKKQKVESQQNTIGRRN"
     gene            28300..28743
                     /gene="dut"
                     /locus_tag="PKX59_02490"
     CDS             28300..28743
                     /gene="dut"
                     /locus_tag="PKX59_02490"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOZ89765.1"
                     /translation="MQNKTEVKIINKSSHPLPAYESNSAAGMDLRADLKEAVELKPLE
                     RALIPTGIYIELPQGFEAQIRPRSGLAAKHGVTVLNTPGTIDADYRGEIKVILVNLSS
                     VAFTINNGERIAQMVVAAHQQVVWKETLELSETNRGAGGFGSTGK"
     gene            28826..29557
                     /locus_tag="PKX59_02495"
     CDS             28826..29557
                     /locus_tag="PKX59_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOZ89766.1"
                     /translation="MIESLVIIPTYNEKENIQKIIKAVFNLLNTFHVLVIDDNSSDGT
                     ADLVKQLMPQFPDSLHLIQRQGKMGLGTAYIHGFKWALERNYQYIFEMDADFSHNPAD
                     LQRLLDACKNGGADVAVGSRYINGVNVVNWPIGRVIMSYYASVYVRLITRMPVRDATA
                     GFKCYRRKVLETLDLSKIKFMGYAFQIEMKFAAWCSGFRIIEVPIIFTDRTEGKSKMS
                     SGIFKEAFFGVIQMKIKSWFRKYTH"
     gene            complement(29781..29969)
                     /locus_tag="PKX59_02500"
     CDS             complement(29781..29969)
                     /locus_tag="PKX59_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOZ89767.1"
                     /translation="MQNGTVKFFNETKGFGFIKAENGEEIFVHSSGLKEQIRENDNVV
                     YEVQQGKKGLNAVNVRLA"
     gene            complement(30009..31130)
                     /locus_tag="PKX59_02505"
                     /pseudo
     CDS             complement(30009..31130)
                     /locus_tag="PKX59_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(31418..32506)
                     /locus_tag="PKX59_02510"
     CDS             complement(31418..32506)
                     /locus_tag="PKX59_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOZ89768.1"
                     /translation="MNKNNYCVIMAGGIGSRFWPMSRNAFPKQFLDILGTGQTLLQQT
                     FNRFTKICPIENIYIVTNESYIDLVKKNLPQLPVENILGEPLRRNTAPCVSYAAFKIA
                     AKNPDANIVVAPSDHIITKEDAFIEAINKGLTFTAQNDCLLTLGITPSRPDTGYGYIQ
                     FVDEKHNEINKIFKVKTFTEKPNLEMARFFLQSGEFVWNAGIFLWNLKSILKALKEHL
                     PEMYHLFEEGNSIYNSKGEDAFIKTAYSQCTNISIDFGVMEKAKNVFVMSADFGWSDL
                     GTYGSLYEHITHDANKNAVVGKNVMLYDTKNCIVNMPHDKLVVLQGLSDYIVVEAENI
                     LLVCRKQDEQQIRQFVNDVKSAKGEKFV"
     gene            complement(32713..36219)
                     /locus_tag="PKX59_02515"
     CDS             complement(32713..36219)
                     /locus_tag="PKX59_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOZ89769.1"
                     /translation="MLKRFLFIMLLCTTTMAMAQTDYRLMFLGKAVLPAENLNSFIQE
                     KVSENDVYNGYYYRIIQFYTVPTQAEKAVMETAGIKLLEYLPKNAFNAAIPISFNKQL
                     LSGFNVRSVTNQSSEQKLSISMLGDYPKYAVPSAGMVDVRLQFQKNISIQQAQQIAAT
                     YGKILSSTEINNMVEVRMQQQSVATISQQPWLFFIDLTSPPSVKDDLEGRSLHRSNVI
                     NNDLLMGRKYNGSGVAAALADDGFVGPHIDFTGRITNYTTTVGPSHGDMTGGILAGAG
                     NLNPAYPGMANGVQLHVFDIGSYPQIIDAVANYNNLGTVVASTSYSQGCNEYTTDTQF
                     GDQTIHDNGQLEFVFSAGNNNGTNCGYGAGGNWGNITGGYKQGKNVIAVANLDALEVI
                     DPSSSIGPASDGRVKPDIAANGRDQMSTDENNTYQVGGGTSAACPGIAGICTQLIQAY
                     KQINSATDAPTALIKACLLNGAEDIGNPGPDYKYGWGRVNALRAVTTIEDARYFTDSI
                     NQSDSLTYSINVPAGTSQMRVMVYWHDVGGSPASSTYLVNDIDMQVTDPSNAVWNPWV
                     LDPTPNAANLNAPATRGIDHLNNMEQVTLDNPAAGNYVVSLKGFAIPQGPQTYYVVYE
                     FRNDDITVTYPMGGEGFVPGHPEIIRWDALKGLGSFDVEYTTDNGTTWNNIATVNQNT
                     LQYSWNVPNNIATTGDARVRVTRGSVSGLSDTTFTIVGIPQNLNVIYSCPDSIKIGWA
                     AVSNAAWYEVSMLGTMYMDSVATTTNTDYIFTNLIASNSYWFSVRAVMANGNKGRRAN
                     AIKKQLGIFNCPNIPPSVQFNANFNVGCTSKTFTFTDQSSNAPSSWNWTFNPNTVTFV
                     GGTSATSQNPQVQFNTPGTYDVTLDATNGIGTSSSTQTAMINILAPSLPPMTQDFQAT
                     VFPPANWSIDSSNANYTWLKSALITGSAGTSTYAARFNNYSYNSSGSEDGLISYEVSL
                     SNAAAALLTFDVAYAQYSSVYNDGLRVDISTDCGNTWVPSGYLKTGVALATTGTTTSN
                     FAPNAANQWRNDSVSLNAYLGQDVSIKFVNINDFGNSMYVDNVNLDVTTGLNQLDNNV
                     GTVNISPNPSDGKFIIYSKQTSHESYTLTVYDINGKVIAEDVFPANNKSHAVDISNFS
                     KGVYFIKVEGEKSVQRLKVISR"
     gene            36372..37184
                     /gene="bamD"
                     /locus_tag="PKX59_02520"
     CDS             36372..37184
                     /gene="bamD"
                     /locus_tag="PKX59_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOZ89770.1"
                     /translation="MKNRLSVYFLLMALLLTLGSCSKFNRIRKSGDMNMKYDAAVKYF
                     ENKKYFNALQLFEELIVVLKGTDKAESSYYYYCLSYFYTGDYTSAAYHFNNFAQTFPM
                     SAKAEDALFYNAYCYYLDSPPSSLDQQSSLDAIRQFQLFINKYPTSQRVAECNKYIDE
                     LRSKLEVKAWDNAMLYYKMEEYKSAIVAFENVVKDFPTTKYQEECLFLSLKSAYLYAQ
                     NSIESKKEERYKSTIDHYNKLIEIFPATEYKKEASRILSAAEEKLKDYHTVN"
     gene            37194..37532
                     /locus_tag="PKX59_02525"
     CDS             37194..37532
                     /locus_tag="PKX59_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOZ89771.1"
                     /translation="MNSRNVAQTTVTRDVVDFEKKTENIYESLAIIAKRANHIAAETR
                     EELHDKLSEFASSTDTLDEVFENREQIEISKYYERLPKPTLVAIQDFLDDKIYFRSPA
                     KEENKDSAEN"
     gene            37537..38742
                     /gene="coaBC"
                     /locus_tag="PKX59_02530"
     CDS             37537..38742
                     /gene="coaBC"
                     /locus_tag="PKX59_02530"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOZ89772.1"
                     /translation="MLQGKKILLGVTGSIAAYKTPELVRQLVQQGATVKVIVTAAAKD
                     FVSPLVLSTVSKNETLQTFTSEDNSTWNNHVELGLWCDIFLVAPLSANTLAKFANGIC
                     DNLLTAVFLSCRNTVMVAPAMDVDMFKHPSTQKNLEIIAKNNIKIIGPESGELASGLT
                     GKGRMTEPEDIVQEVINYFNPSLPLRNKIAVVTTGPTQEALDPVRFIGNHSSGKMGVA
                     IANCLAAKGASVILISGPSVSLGNLNKSIKIVHITSAAEMLKACKQAQPDASIMVMAA
                     AVADFRPEGISDQKIKKKNNEGLSVQLVPTEDIAAEMGKLKKEGQILVGFALETNDEK
                     QNAERKLKNKNLDFIVLNKLEESNQVFGSDFNRITIIDKNLVTTPFEKKSKDAVASDI
                     VEKIITLVQ"
     gene            38772..39635
                     /locus_tag="PKX59_02535"
     CDS             38772..39635
                     /locus_tag="PKX59_02535"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOZ89773.1"
                     /translation="MSLHTVHAQELNCQVNVMAPQIQNVDKKVFETLQQSIFEFMNNT
                     KWTSDKYLNQERIECSVTITINERVSNDEYKGSIQITSRRPVFQTSYNSPTLNFNDDN
                     FQFKYIEYQPLEFSENGSNPNLTAVLAFYANIILGVDYDSFSLYGGAPYFAKAQTIVN
                     NMQNAREAGWRAFEGTKNRYWLSENMTNPIFKPYHEMFYQYHLKGLDVMAKKKDDGLS
                     QISESLDLLMSVHKEKPGSLLLQTLFYAKADEIVNIYSGAYPDQKVKVSNLLNEIDPA
                     NGTKYSKIMGN"
     gene            complement(39665..40063)
                     /locus_tag="PKX59_02540"
     CDS             complement(39665..40063)
                     /locus_tag="PKX59_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89774.1"
                     /translation="MIKVREFVFLILIGAVVLLSAFKIQQDGKDYGQCLQKVNKKFGA
                     ETLSCPASTKPYKVWFVNTCSDTLAVKLAVQEKSKRWRTFTRTQLLPGDTISGYACTG
                     VGKYLYYTKQYKDNSVDLPSDEVIDNSNKE"
     gene            complement(40060..40614)
                     /locus_tag="PKX59_02545"
     CDS             complement(40060..40614)
                     /locus_tag="PKX59_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ89775.1"
                     /translation="MKIQNHLNNVKILFIVLLSAFCVQANAQVDSADFKMRIDSLMNQ
                     GKIKFNNGKFQEALLDYTYVANMDVANVNAILQQGFCNTILKKYTEAIGNFTDVIFYE
                     PNHEWAYVSRGSAYNKIEKYDKAIVDFNKALELKPEDAEAYNNRGFAKKMLGDIKGAC
                     EDWNKSKKLGNEEAKIILKNNYCK"
     gene            complement(40589..41071)
                     /locus_tag="PKX59_02550"
     CDS             complement(40589..41071)
                     /locus_tag="PKX59_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89776.1"
                     /translation="MVYDRINKEFSKRRKFFLLLLMLSVFSPALRAQENITVQQTLDY
                     INQKFAGKYVVSINYGVLIATYFDGETKYREDQAAIKDLDATKVYYNSKAGMLLINCT
                     NPQKKCVTREFLGKRLFYSRISFPVSYGQKTNDGLVKAFQHLIHLINDKNYENSEPFE
                     "
     gene            41164..42363
                     /locus_tag="PKX59_02555"
     CDS             41164..42363
                     /locus_tag="PKX59_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89777.1"
                     /translation="MFGIGKRIHLVPLGVFLTIFMFIHGVSTAQTKKTLTINAKFKID
                     GGGSLGDAYMVLQRDGQSIETLPGQSSYTLNLEIGADYILSFNKPGYITKKININTTG
                     ADEERVEQGFESYPFTVVLMKQYEGVNIVVFNQPVAKITYSKKYDEFDYDTDYTKSIQ
                     SAIQKAEDDLKKAEKEFKKNPPKPAKDSTIVANKPKAEEVKPQKEKTKTAPQEEPVAV
                     KEKGKNDDTKNESKNSNKSAAATGGQDQPLKPTQGGGSDEPEGNIDIEKGADQKADIE
                     PTAGNDAIKNKIGLTKGADQPPAAEPAKPQPVVIKTPETAAPEKPKEVVVHTAPEPPE
                     NVSVRREQLAEDRKTVYNVWVTKGNKTVLYSKVVYAWGGIYFFRDLNTSISENMYKQA
                     DAGIVNF"
     gene            42508..42675
                     /locus_tag="PKX59_02560"
     CDS             42508..42675
                     /locus_tag="PKX59_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89778.1"
                     /translation="MNLFKPQNDSQVSLLEDIKTLRSLKGNTSRFAILRSVAKTKKHT
                     RTVYPEGWKFE"
     gene            complement(42692..43192)
                     /locus_tag="PKX59_02565"
     CDS             complement(42692..43192)
                     /locus_tag="PKX59_02565"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOZ89779.1"
                     /translation="MNLVCLPEEVERLASLREQETLDYFKSIKKKYKSAVIDKAVHLL
                     YAEIAPKIDCTACANCCKKQSPVFTEEEIRPILNHSGISEHENLNELLEHDEINHHYI
                     LRKNPCFLLNKNLCSVYTLRPSSCSEYPHLNRPFFIYRNKSNMSNYKICPIVYNVVEA
                     LKNSLS"
     gene            complement(43203..44342)
                     /locus_tag="PKX59_02570"
     CDS             complement(43203..44342)
                     /locus_tag="PKX59_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOZ89780.1"
                     /translation="MKVYFDNAATTPLRQEVIEAMLPVMQKIYGNPSSIHSFGREMRS
                     AIEKARKTIATLLKVAPSEIFFTSGGTEANNMAIRCTIHDHKITHAISSPIEHHAVTH
                     TLEDLEKEGIKLSWVKLNDKGEADYEHLEELLKNNPRSFVSLMHANNELGTVNDIDRI
                     GELCKQYNAIFHCDTVQTMGHFPYDMSQKNIDFFTCAAHKFHGPKGVGFIYINHKLKI
                     HPLITGGAQERNMRGGTENAYGIIGLAKALELAYAELEEDRKHIQGLKEYMKQRLQEE
                     IPGVAFNGDVSARSLFTVLNVSFPPYEGKDMLLFNLDISGIAASGGSACSSGSEIGSH
                     VLRGINADPERPAVRFSFSRYNTKAEVDFCMDKLKALFAVTAVVK"
     gene            complement(44374..45780)
                     /gene="glmM"
                     /locus_tag="PKX59_02575"
     CDS             complement(44374..45780)
                     /gene="glmM"
                     /locus_tag="PKX59_02575"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOZ89781.1"
                     /translation="MTLIKSISGIRGTIGGKSGESLTPVDVVKFSAAYATWLAAQFPA
                     KTKCKVVLGRDARISGPMIHNLVCGTLAAMGIDVIDVGLSTTPTVEMAVTFHQAHGGI
                     ILTASHNPKQWNALKLLNSKGEFISAADGETLLKIAEQESFEFANVNQLGTVTTDAHA
                     IDKHIAAILALPLVDVKAIASKKFKIVIDCVNSSGGIALPPLLKALGVEEVIELYCTP
                     DGQFPHNPEPLPEHLTELSKTVKKSKAHLGISVDPDVDRLALVCEDGEMFGEEYTLVA
                     VADYVLKNKKGNTVSNLSSTRALRDVTEKAGGSHYASAVGEVNVVEMMKAKNAVIGGE
                     GNGGVIYPDIHYGRDALAGIALFLTHLAKYGNTVSMLRSSYPDYFISKNKIELTPEIN
                     VDGVLESIQEKYKKHKINTIDGVKIEFDKEWVHLRKSNTEPIIRIYAESDSITTANNL
                     AEKIISDIKELISHPAKV"
     gene            complement(45896..46840)
                     /locus_tag="PKX59_02580"
     CDS             complement(45896..46840)
                     /locus_tag="PKX59_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ89782.1"
                     /translation="MMSKEILLIDTFHETFIPKLTAMGFHCTEGYEWSLEKIQQSIGN
                     YEGIAIRSRIPLNKDLLQHATNLKFIARGGAGMENIDVAYAVSKNIKCLNAPEANRDA
                     VGEHALMMLLALLNKLMKSDKEVRNGIWLREENRGTELQGKTVGIIGFGNMGSAFAKK
                     LAGFEVNIVACDPYKKIDTAEYPFIKQVSVEQLQQESDIISLHVPLTAETKYMINDTF
                     ISQCKKEIYIINTARGKCLNTADLVKHLKTGKVKGACLDVHEYETSSFEKLETLPEPF
                     GYMIKSDKTILTPHIAGWTHESNRKIAEVLVKKIEQLS"
     gene            47332..47529
                     /locus_tag="PKX59_02585"
     CDS             47332..47529
                     /locus_tag="PKX59_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89783.1"
                     /translation="MHALDILLLGGAVVCFVLAYIIASRPARELRQSNKSKSKWNHVN
                     MDSASSVHSPEMMKYVHDKTG"
     gene            complement(47556..47687)
                     /locus_tag="PKX59_02590"
     CDS             complement(47556..47687)
                     /locus_tag="PKX59_02590"
                     /inference="COORDINATES: protein motif:HMM:NF040055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89784.1"
                     /translation="MEELAKDGAILEHARNAVINILNDDPLLNSEENKIFYRHLHQL"
     gene            complement(47778..48314)
                     /locus_tag="PKX59_02595"
     CDS             complement(47778..48314)
                     /locus_tag="PKX59_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89785.1"
                     /translation="MKIPIKLKGYYSQLEHIETNVGYPPNVKKVVDTIARNVVFYEDG
                     VFLYSFYGEQFKGNNPIGYKGGVALWGGYSIFNDTLKGFFMTGPDSRPNRPWGYVWFK
                     IIDKNTIRKICFKWQEPITAADVFEFKNTRAKNISNATFIPSDSLPNPNLSWIKNKKW
                     FWCDEQQFKLWKKAQKEK"
     gene            complement(48416..48634)
                     /locus_tag="PKX59_02600"
     CDS             complement(48416..48634)
                     /locus_tag="PKX59_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89786.1"
                     /translation="MLSEVKLRYTKTYEVREYSSSLVQDRTQNWVHKNGFFYLPFGKA
                     PYYKDYSQFKSGMYFYGGYNNPYSLWGK"
     gene            complement(48744..49490)
                     /locus_tag="PKX59_02605"
     CDS             complement(48744..49490)
                     /locus_tag="PKX59_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89787.1"
                     /translation="MKTEILRRQIFILLIPVFIMGCFTSHKRFNLFFENKTDSLSIKL
                     KIKGIYYLANEPIDRCIPTAYIFLFNNGMAYIRNTGQNNRFWVKNEFGFDKDKPWLTR
                     PLDKPFIYKIDSVFWQNPDSILVNTIKSTFKFSPFNKEWWGTYSIKNNTIKIEYFSKT
                     NQESIIHNRYLIELSGYLKNDSTIIITNRICGKWYHCYQCKSDLKNNLNSYTPPVQYN
                     FYSTDFKPDSTQAWFLKKHWYKKGLHQSRK"
     gene            complement(49806..50480)
                     /locus_tag="PKX59_02610"
     CDS             complement(49806..50480)
                     /locus_tag="PKX59_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89788.1"
                     /translation="MKFIKYLFCCLIIVHITIAKAEDSIANKKIVLKIAPINFVDQIS
                     HQSAMIGTEYWLGTRYGFEISFAPIFGRDYFNGYGGTGFKSSVEVKRCFSMKGKRLFY
                     LGTKLFYNKINYASKNLFENKITSEEYEEKYFIRKSVYGANITSEVEWRLFPKIILNI
                     NAGIGLRVKNVRHYQRLRPEDNFYSVDVLAENVRDKKGNYVLPDMAIGIKIGYAIDRI
                     KKVQDN"
     gene            complement(50819..52912)
                     /gene="recG"
                     /locus_tag="PKX59_02615"
     CDS             complement(50819..52912)
                     /gene="recG"
                     /locus_tag="PKX59_02615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOZ89789.1"
                     /translation="MSYFQKDIEFVKTVGPQRAAVLRKELEIHNVSDLLYHFPFRYVD
                     RSKFYTIAQLNDDLPYVQIKGVITSLTAFGNKRTTRLQAVFKDESGSMDLVWFQGITW
                     IRDSIKAGTSYVVFGKPALFNSKWSIIHPEIEELEKFTAQPFKGFQPFYYSTEKSKVK
                     GIDSKGIMRIMRMALKDLPPTIEETLSPQLIKTFNLMPRHDALIQIHFPENEDLLRKA
                     RFRLKFEELFYIQLKLLLNKQNRTATQNGRMFTEVGYLFNTFFNEHLPFELTQAQKRV
                     LKEIRKDTISGKQMNRLLQGDVGSGKTVIALFAMLLALDNKQQACLMAPTEILAAQHF
                     QTITSLLSSLPVNVELLTGSLPESKKKAVIERISSNETHILIGTHALIEDRVKFNDLG
                     IVIIDEQHRFGVEQRAKLWMRQQPPHVLVMTATPIPRTLAMTLYGDLDVSIIDELPAG
                     RKPIKTMHLFESSRLRVIGFMKEQIKLGRQIYIVFPLIEESEKLDLQNLMAGYDSIQR
                     DFPLPEYFVSMVHGKMKAKDKEQEMERFVNGKTHIMIATTVIEVGVNVPNATVMIIEN
                     AERFGLSQLHQLRGRVGRGAEQSFCILMSGNKLSADARERLKTMVQTNDGFEIAGTDL
                     KLRGPGDITGTQQSGLLNLKIAELAKDGAILEHARNAVINILNDDPLLNSEENKIFYR
                     HLHQLREQPNWSKVS"
     gene            complement(53043..53237)
                     /locus_tag="PKX59_02620"
     CDS             complement(53043..53237)
                     /locus_tag="PKX59_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOZ89790.1"
                     /translation="MKTGKVKFYNNSKGFGFIVEDEGGRDLFVHQTGLIDRIADNDLV
                     TYEVVQGKKGLNAVNVKKQQ"
     gene            53338..54954
                     /gene="ade"
                     /locus_tag="PKX59_02625"
     CDS             53338..54954
                     /gene="ade"
                     /locus_tag="PKX59_02625"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOZ89791.1"
                     /translation="MTTSGNIIDIFNRRIFPGTVHFSKGIITEITEDNAVYDQYLLPG
                     FTDAHIHIESSMLVPYEFAKVALLHGTVATVSDPHEIANVCGLKGVEYMIENAKDAKL
                     KFNFGAPSCVPATTFETAGAVLNAEDVAQLLARPDIRYLSEMMNYPGVLFGDIEVMKK
                     IESAHRLNKVIDGHAPGLRGDDARKYIAAGISTDHECFTLEEALDKVNGGMKILIREG
                     SAARNFEALHTLITSHTAMTMLCSDDKHPDELLHGHLNQLVVRALNKGHNLFDVLQCA
                     CVNPVLHYSLSVGLLRVGDPADFIVINNPEVFLVSQTWINGECVANDGQTTLTNRSHA
                     PINNFNAWDVSVQDIEVKKNSESVKVIEALDGQLITNCIQAKPLEKDGLWVSNPENDI
                     LKIAVVNRYSKQKPVVGFIKNFGLKLGAIASTVAHDSHNIIVTGVSDADIVDAVNLLM
                     QSGGGLSAVSGTDKKVIALPVAGLMSDKSCEEIGRDYASIDSMAKQMGSALRAPFMTL
                     SFMALLVIPHFKIGDKGLFNAVKFELTSLE"
     gene            55001..55252
                     /locus_tag="PKX59_02630"
     CDS             55001..55252
                     /locus_tag="PKX59_02630"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HOZ89792.1"
                     /translation="MWQEVNNSLERTFIFNDFKQAFAFMTHVAFIAEKMNHHPDWSNV
                     YNKVTIKLNTHDAGSVVTEKDHKLAAAIDVVFNQINKKV"
     gene            55249..55623
                     /locus_tag="PKX59_02635"
     CDS             55249..55623
                     /locus_tag="PKX59_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="HOZ89793.1"
                     /translation="MSRFKKFFIWVVVLAIIGTALYFLAGSYTYSEGTQTGYLVKFSR
                     QGYVFKTYEGELNLGGMNAESNSVVNNLWHFSVDEDDKAAIDSLRVSEGRVIKVYYRE
                     IVRNYAWQGETPFFVYKVEKVK"
     gene            complement(55628..56197)
                     /locus_tag="PKX59_02640"
     CDS             complement(55628..56197)
                     /locus_tag="PKX59_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89794.1"
                     /translation="MQDIQPFYNWVDLYSSAEDELSPFYGRVYSEFEYSNTIYNYFIH
                     PQWDDFGSPTLYIKIIYCDYEKSFAIIELMGEWNDAINNDIMTLKRDVIENLMEHGIS
                     HFILIGENVLNFHNSDDCYYQEWFEEVNETGWIAAVNFRDHVLSEMQRANIDYYLLFG
                     GELDNLNWRTYRPSQLMAKIDSIIRHRIG"
     gene            complement(56250..>56439)
                     /locus_tag="PKX59_02645"
     CDS             complement(56250..>56439)
                     /locus_tag="PKX59_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOZ89795.1"
                     /translation="AVMIKLGHVKGNKMVDMQLTNNKLVSRGIRMIMDELGVPEKKAT
                     LMLKKYGSVRAAIKNHKS"
BASE COUNT        17146 a        10137 c        10620 g        18436 t
ORIGIN      
        1 taataaattt attgatcaaa aagtaaatta catacacaac aatcctgtag aagcatgaat
       61 agttacagag ccatattttt atgtgtatag cagtgctgat caatttaccg atttaaagct
      121 ggctgaacta tgaagtccga tagctattgg acggccggag gccccaagga taaaaggcac
      181 gagcggaaag gacgctcacg actacttaga gacctaataa aaaataagaa cctaaggatt
      241 tgtgaaattc aattgataat gtattgtaaa tagcttcggc tttatcaaag ttgttgtttt
      301 agaactctga ccaagctaaa tccatttttt cgctatactt attagcaatt tgattctgcg
      361 tttcagccat tgactttctt ttatctaggc cccataaatc tatcaccatt gaaatgaatc
      421 atgtggtcag gcgtgtctgc cagccatact tctgtttccc aagcaatatt attggagtgc
      481 tttttaaatt cagtgaaatc aggaaatgct gttacataaa tttttccggc tttacagcct
      541 ttcagaaatt cttcaagttc aacaattcgt ttcggagaaa cagggccatg tgaagtaacg
      601 gcttcaatca ggaataacca gtttttcttt tcgtcataaa tcacaacatc tggcaattta
      661 ctgtgctggt caatcggtat tcctaattct ttcaacttct tttcatcaac gaacaaatcc
      721 ttttttgctg tatcgcctaa atagagtatc gaaccaccat tagcaaatct tgcagcaaaa
      781 ttatgcacga ttgcagcctg aacttcgttg tgctttcctg acgaaagttt aatcgttttg
      841 ccattgctta gttttaccgg aatcagattt tgttttcttt cttttttata tcgtttcgct
      901 aattccccgg cttctgattt gaatttttca actgctttct tccaatcttt agttccaaaa
      961 ttttgaatag cttctaatgc ttcattggta attgcataat gagcattcgg actatttacc
     1021 ggtagttttg gattgtccgg attgtaatct gcaatacgag cctgaacgaa ttgatgtaaa
     1081 acctgccttc tgaatgtctc tcttgtattc ggtgcgtatt cttttttatg cacatcgaga
     1141 acgaaagcca taataccttt tgtaactttt aaacttttgc gagtcgcttt actccaacta
     1201 ttacgaggct taattccgca gagtgccaat aacgtgtatg ccgatatttc attttgctgt
     1261 gctgttggca accctaattc tttgagtatc ttttttgctt cgtcaagttt gctcatattg
     1321 ttgctaatgt aaattcaaat tgttcattta ctattttgtt ggctttatct actgaaaaat
     1381 cattagaaag aataacgcta tttccaattt cttttattgt ttcaagtgga ggaaggggaa
     1441 tttccctgag ttcggtagcg cttacattca cgttgccgtt gaatattctg aaatacgaat
     1501 cgaataaact gctgttcagt aaagcacaaa gcccaatcac ctcatttcgc tctaaatgtc
     1561 ctttcggacg atagatgtaa tttactttgt tttctacacc gatatattct gcttcaatga
     1621 aattgcagaa gtatggggct gcaaccaatc tgcttttgtc atccttagca ctgaaacgtc
     1681 tcaatagaac atagttttta ttcggaataa gaatggattt tgattcatca tgtactctta
     1741 catattgtcc tttttcgggc tttggcatgg gccaatccaa agccatttgc tgtacattgt
     1801 gaagccaaaa caatggagct aataatactg ttccgttttg atatgtatcg tgtatatgat
     1861 cttttgacct gaatgcaaca acggggccgg ttgaaatctg aatattgtat ttattcaggt
     1921 tgccgctcca atttttaaaa acattcaaaa tagcttcatc tgaatcactc gttggtaagt
     1981 acaatatttt ttcatttgag ctcaggtcaa tcaattcctt ttgtgcgaaa gatttgatta
     2041 ttggagaatc aatatccttt aatccatgcg atgaataaac atgtactttg tgctttggat
     2101 ttgttttttc tttacgcgtt ccttttatga taacggtttc ctgcaatacc ttatctctgt
     2161 tgaatgtgtc ttttcgggaa acaaacaaat gcacgttgtc aatttcaatt attttgaaaa
     2221 aatactcccg gaataatttg aagtaactgc cagaagcgta gcttctcgga gttatgaata
     2281 ttagttgccc attttctttt agtagccttg cggaaatagc cataaaaatg gcgtaaatat
     2341 tcggatgtcc attaacaact gcttttgcag caatcgctct tttgtcgtca ataggtagtt
     2401 tgaagtaggg aggatttgaa acaataatat cgaacggatt aattgtctgt gaaaacaagt
     2461 tgccattatc tgttaaacaa cctgcgtttt caagaatgaa atcatctgta tgaatagaga
     2521 attttaattc aatatttttc gcatttaacc aacttcttaa ataatctaat gactcttttg
     2581 tataaggaat taaagctttg tctgtttcgt acacaatcaa gtcaatctcc tgcaatttgc
     2641 tattgttctg aactatagat tcgattaatg cacaagtcag aatagccgtt ccgcaaccgg
     2701 ggtcaagtat tctaagagaa ctttcgctta cttctgcaaa tgtccccatc agtccggcta
     2761 tttcaatagg agtgaaaaat tgtccgtttg tttttttatg ttcctgattt accgattggg
     2821 cataattcac cccaagccgg tcagcgcaat ggctcggcaa ctcatcagca attttttcta
     2881 tgagtttttt atctgccatt tttcttttta gcgatatagt taattttctt ttcggccact
     2941 tgcatagctt ttagcgctac ttcttcgttt tgcagttcgt aaacgagttt gtcgctgatg
     3001 aacgcgatca aaaattcatt caggtcaact ttataaaatt tagccagacg aataacctga
     3061 tcttttgtcg gcattcgttc accacgttca attttgctta aaagagcctg gtcaatttct
     3121 atttccgcag caacctgtcg aagcaatagt ccgttttgct ctcttaattc tctgattatt
     3181 tctgctatat ttttcaactt gatatgattt gacttgacat ttaatgtcca aaaatagtaa
     3241 aatgattttt gtagccaaag aataattatt aacaacagca ttaactagcg ggcgggtgaa
     3301 ttttggtgcg tattgaaaat tgcgggcttg tgcccttcag ggattgaggg tgagcagagg
     3361 agaaaagaat tgtttaaaat acaaatatcc tcaagcaatc gttacttttc gcagacctaa
     3421 acatgccaca catcaaacaa agcctgtgaa attaataatt ctgaattcta cattctacat
     3481 agtttcatta tcattatttc ataactgtat aacaaaaaag gccatctcaa aaatattttt
     3541 tgagacagcc tcctgaacca aactatcaaa tattttactt cttcaccaac tgaatttctg
     3601 cagcaatctt cacttcttcg ctaaccaata caccaccggt ttctaaagct gtgttccagt
     3661 tcagtcccca gtctttacgg ttaatttttc cggataatgt aaatccagcc ttttcctgac
     3721 cccatgggtc tttcatcata ccaccaaact ctgcaatcaa ttttacaggt ttagaaatac
     3781 ctttcattgt aagttggcca ttaagtgcgt aagtgtcgtc accggttttt gaaaaattct
     3841 ctgacttgaa tgaaatagaa ggatgttttt ctgcatcaaa aaagtcaccg ctctttaagt
     3901 gtgtatcacg ttgtgtgtcg cctgtggtaa tgcttgatgc atccatactg aaattgatat
     3961 ttgcattatc aaaattgctg tcttcagatt gtactgtacc ttcaaacttt gcaaagtttc
     4021 cttttacatt cgatatcatc agatgtttta ctttgaatgt aatttcagag tgtgcctgat
     4081 ctatgcccca tgttgtcttt gtgagtgttg tcataatttt atttttttaa attgttaatt
     4141 gtattgttta attgagttgc attggaatat ccatcagtaa tatttctgca tcttcggttt
     4201 tagcgataaa gtttatttca gaggtattcc ataaccctat ggcatcgcgc tcatgcaaca
     4261 aattgtggtt tatttcaaat tcgcctttca ctaccatagc atatacaccg ttaccttctt
     4321 tttttatttt atatgaggtg cttttgtttt tatcgaagtt gcccatactg agccatgcct
     4381 gttggtgtat ccacaatccc tctttttctg tatcgggtga aacaatagtt tgcagtttat
     4441 tttttctgtc attgacattg agtgatagct gatcgtagcg gggttttaca ttttctttat
     4501 cgggataaag ccatatctga aacaacttta ccgcatctgt tttacttgca ttaaactcac
     4561 tgtgtgtaac acctgagcct gcactcatca cctgcacttc gccatatttt atgatgccat
     4621 gattgttcat gttgtcttta tgttcaagcg caccagctaa aggaatggta ataatttcca
     4681 tgttgtcgtg tggatgtgtg ccaaagccca ttcctggttc aacatgatcg tcattgagta
     4741 cacgcaacac gccaaagtgc atttgattgg gattatagta gcttgcaaaa ctgaatgaat
     4801 gttttgcttt taaccaacca tggtctgcat ttcctctgtt ttctgctctg tgaattactt
     4861 tattttccat atctttaaaa tttatggtac aaaagtagag cgaccttatt ctttcagcaa
     4921 taacctgaat taataaagca acttaatgta ggttaagtta atatgtgtga gtaagttttg
     4981 gtgtcagttt ttagtcatgc gtgcacgcat cttactgaaa aattccggtg tgacaccaat
     5041 ataggaagct atatatttct gtggtaaaca ggaaaccaaa gcaggatact tcagacaaaa
     5101 cttttcgaat cgttcttctg cactcagaga gagtttatcg agtattcgct cttgctgact
     5161 tgccatgctg ttctcaatca gtattctgaa aaaacgttca aacttgggga ttttatcaaa
     5221 aagtaaatca cggttttctt ttgtgagtac aacaatttca ctttcatcaa tggcgtcaat
     5281 aaataaaatt ccaggccggc cagttatgct actatagaaa tcgccaatcc accatcccgg
     5341 tggtgcaaat tgtaaaatat gttctgttcc gcttttatca atattataag cacgcaaaca
     5401 accagaattg acaaagcatg atgcagtaaa cacttcgcct gcctgcataa caaattgctt
     5461 gcgtctgact atgcgggcac ttagtaatga tgtaaatatt gcttcttctt cttctgaaag
     5521 agaaatatat cttgaaatat ttttcagaat aagattaaaa ggtttgttgc tatctgtttt
     5581 tgccatgctg ctcaatataa tcaatcaggg catgaataat tttaatatga atttcttgtg
     5641 ttctgtcggc aaagccggaa tgtggaacac gaatttcaac atcacaatga ttttttaatg
     5701 ctccgccatc tttacctgtc aatgcaacaa caataatatt ttttgcttgc gccacttttg
     5761 ctgcttccaa aacattggca gagttgccac ttgtgcttat ggctaataaa acatcacctt
     5821 tgttgccaac tccttcaaca aatcttgaaa aaacataatt aaaaccataa tcattaccca
     5881 cacaactcat ataggatgga tcagagatac tgattgctgc aataggtttt ctgtcgttac
     5941 gataacgccc agccaattct tcggcaaagt gcatggcatc gcacatgctg ccaccattgc
     6001 cgcacgatat aattttgcct cctgctgcga gagaggcagt catcaattca ccggcttttg
     6061 ttacggctgc aatattttta tcgaccgata aaaaagtttc aagtactttt tttgcttctt
     6121 caaaatgctg acgaataatt tctgacatat ataagtttat atagcacaaa gatagcaggt
     6181 tatttgaact gaattcagaa caattgattt taaaagtatt ggttattgat tgaaaaaatg
     6241 attgtgatgt aaattgtttt tatttgcagt aattcctttc tatgcaggta aagaaaaagt
     6301 tgattttatc attgctttcg gcagtgatgc tttcgttagg ctggcccgag cgcggcttaa
     6361 caccacttat ttttattgca tgggtgccat tgctgctttt ggaagagtac agtcatcaga
     6421 aaaaagaaca gaaaaaattc gggttgtttg gctggacgta tttaaccatg ctcggatgga
     6481 atgctttaac aacatggtgg gtttgcaatg ccagttttgg tggaggcctg tttgctattt
     6541 tctgcaattc attatttatg acatgtgtgt tttatgcttt tcatattgtt aaaaagagaa
     6601 aaggacatgt gtggggctat acagcattaa taattttttg ggttgctttt gaatacctgc
     6661 atctgaactg ggagttaagt tggccatggc tgacattggg taatgtattt gcaacaaaac
     6721 cacaatggat tcaatggtat gaatatacag gagtgttagg aggcacagtt tgggttttgt
     6781 tggtgaatat tttaattaca agaatttttg ttgcatcagc agataaattg aaacgcagcg
     6841 tgctgtatgc aactgttgca atagcttttc cggggttgat ttcattgctg cgctactaca
     6901 cttataagga aacggaaaat cctgttaatg taattgcaac acaaccaaac attgaccctt
     6961 atggtgagaa attttcgggg atgagtaacg aagagcaatt aattcctgta ttgcgtttgg
     7021 ctaatctgga gttgaacgat tcgtcagatg taatacttgc ccctgaaact gcaattgcca
     7081 attccgtttg ggaagatgag ttggagttat atccggaagt gaagttgatc agaaagtatc
     7141 tccaggttaa cccacgggct actttaatca ttggtgcatc aacaaaccgt gcttatctga
     7201 atggacaaaa gccatcttca acagctagaa aatttaaaga tcaggatggt tattatgatt
     7261 cctataacac agctttactt tttgaagatg gaaagcctgc acagattttt cataagtcga
     7321 agttggttcc gggtgtagag aaaatgcctt atccaaaaat tttcggcttt ctcgaaaaac
     7381 tttcaataga tatgggaggc actgcaggaa gtctgggtat tcaggatgag ccaactgtgt
     7441 ttactacaaa agataaaatg attatagctc ctgttatttg ttatgaatca atttacggag
     7501 gttatcttgc caagtatgtc cgcaaaggtg cgcagtggat tgcaattatc actaatgatg
     7561 gttggtgggg caacactccg ggctaccgtc agcattttaa ctatgcgcgg ttacaggcag
     7621 tttctttgcg aagaagtata ggccgttcgg caaataccgg aatcagcggc tttattaatc
     7681 agcgtggtga tgcatttcaa caaactcctt actggcatga tgccgttgtg cggcagacca
     7741 tcaacaagaa cgataaatta acattttttg cacagtatgg cgattatccg ggtctgatag
     7801 cttcaatatt ggtattgccg gttattgtta ttacgttttt cggaaaaaga aaaaatcagt
     7861 aagttttttt cggaattttg ccacataaat tttaatggaa tggaaaattt tgatgtggca
     7921 attattggct caggacccgg aggctatgtg gcagcgattc gctgtgcaca actcggtttt
     7981 aaaacagcgc tggtagaaaa gtatgatact cttggcggca cttgtctgaa tgttggatgt
     8041 attccttcta aagctttgct cgattcatca gagcattatt ttaatgctgt aaataaattc
     8101 agtgagcatg gcataaaaat caaagatatc aaagccgata tggagcagat gattaagcgc
     8161 aaggctcagg ttgtgaaaca aacttgcgat ggcattagct ttctgatgaa aaaaaataaa
     8221 gtaactgtat tgacaggtca tgcaaccttt gtggattcaa aacaattgga tgtagttgat
     8281 gctggtggta agtcaaacag agtaggagca acaaatttta taatagcaac agggtctaaa
     8341 ccatcatcat tacccggaat agagatagat aagaaacgta tcatcacatc aacagaagcg
     8401 ttagagctaa aaactattcc cggtcattta gtagttattg gtggaggagt aattggtctt
     8461 gaattgggct cggtttatgc acgattagga gcaaaggtta ctgttattga atatatgaat
     8521 gctattattc caacaatgga tcgtgcatta ggaaaagaat tacagaaagt gttgagtaag
     8581 gaaggattta cttttttact tgggcataaa gtacaatcgg taaaatcatc agctaaacaa
     8641 gttgaagtcg ttggagaaag cagcagtggt gaaaaggtta atatcatagg agattattgt
     8701 ctggtttcag taggtcgaaa gccatacaca gaaaaattag gccttgacaa agcaggtgtt
     8761 gcacttgatg aaaggggtag aattattacg gatgaaaaat tacaaaccaa cgtgcctggc
     8821 atttacgcca ttggcgatgt aattaaaggt gctatgcttg cacataaggc agaagaagaa
     8881 ggcgtgtttg tggcagaaca actggcaggt caacagccgc acatcaatta cagacttatt
     8941 cccggagtag tttacacatg gcctgaagta gcatctgttg gttacacaga agaagaactt
     9001 aaagaatcgg gagtgaaata taaaacagga aactttccat tcaaagccag tggcagagcc
     9061 agagcaagta tggacacgga tggttttgtg aaggtgttgg ctgatagcac tactgatgaa
     9121 attcttggtg tacatattat tggcccacgt gcggcagaca tgattgccga agctgttgtg
     9181 gctatggagt atcgtgcatc ggcagaagac attgcacgta tttgtcatgc acatccaaca
     9241 tttacagaga gttttaaaga agcttgtctt gcagctactg caaacagagc catacacatc
     9301 tgattaaaca taatacccct aacatataat ttatgaatat tcagataaaa gaaattatta
     9361 ccgtttcaat gatattattc tcggtaatag acatcattgg ctctgtgcct gtaataatcg
     9421 gtatcagaaa aagtgtaggt accattcacc ccgaaaaaac atcgttggta gcattgatga
     9481 ttatgattgt ttttctcttt gtaggagaaa atattctgtc attgatagga cttgatgtct
     9541 cgtcttttgc tattgcaggc tcattcatac ttttttttct tgccttagaa atgattcttg
     9601 gtgtaaagct ttataaagat gaaatgccgg catcggcaag tattgttcca attgcatttc
     9661 cacttattgc aggtagtggc accatgacta cactactttc tttacgcagt cagtatgata
     9721 tgcttaccat tattattgga gtattactaa atatgatttg ggtttatctc tgtctgaaga
     9781 atcttggatt tattgaaagg cttcttgggc caacaggaat agcaatcgtt agaaaagtat
     9841 ttggttttat tttgctagct attgcagtaa aacttttcag aagtaatctc ggaatataaa
     9901 ctcatggcta aaaaaaaatt ctatgttgtt tggaaagggg taatgcccgg agtttatcat
     9961 tcgtgggatg attgtaagag acagatagat ggttttgcag gagctttgta taaaagtttt
    10021 gaaacagaaa atgcagcact taccgcattc caaaaaggat tgtcggggtt tcagaaaaaa
    10081 agtgaatcac aacatttatt tcaagtagaa aaatcgtcat caaaaccaat aaaagaaagt
    10141 attgcagttg atggtgcatg gaatactaaa acattggaaa gtgagtacag aggggtagat
    10201 gtaaaaacag gtaaacaact ttttcttaaa ggcccttatg ctgatggcac aaacaacatt
    10261 atggagtttt tggctattgt gcatgctttg gcttattgca aacgacatca cattaatcta
    10321 cccatttatt ccgatagcat gacagctttg aaatgggtaa agacgaaaaa agcaaataca
    10381 aaattagctt caacgggtcg aaatgaggaa ttgtttgaat taattgaccg tgcagaagaa
    10441 tggttgaaga ataatattta tgcaaacaaa ctgctcaagt gggaaacgca aatctggggt
    10501 gaaaatcctg cggattttgg gcgcaagtag ttgaaaattg atgctttttt ctgttcccct
    10561 ttaaatttat gcaaccaaac acagtttttt ttagaactga aaaagcttga atagcatttg
    10621 ggggtattca aaaacaataa gttgtaagca aaaactgatt tatatcatat tgttacttaa
    10681 ttgctgtcat ataataacta ctttaggcgg agtaggtttg tgaccgtttt ttatgagtgt
    10741 acttattata cttattggag ctagcctggt ggtggcagca ggcttcttgg tttctttttt
    10801 ttgggccgtt cgcagcgggc agtatgatga tgattactcg ccttctgtgc gcatactttt
    10861 tgacaatgac aaaactttaa catcagataa atcaaattca aacataacag ataactaaaa
    10921 ttatgacaat agaaaagttt cgctacgaca acaagattgt tgccatgttt gcttgggcta
    10981 cagtaatatg ggctattgtg ggcatgcttg taggcctgtt agccgctttc caacttcctt
    11041 ttcctgcatt aaattttgat gcacacacaa cttttggtcg tatcagaccg ctacacacca
    11101 atgcaatcat ttttgctttt gtgggtaatg gaatgtttat gggaatttat tattcgttgc
    11161 aacgtttact caaagcaaga atgtacagtg atgtgttaag ctatattcac ttttggggtt
    11221 ggcaattaat cattgtaggt gcagcaatta cccttccatt aggaattaca gccagcaaag
    11281 aatatgcaga attagaatgg ccacttgata ttgcaattgc catagtttgg gtaatatttg
    11341 gcataaacat gtttggcact ttagttcgca gacgtgaacg gcatttgtat gtagctatat
    11401 ggttttatat agcaacagtt gttactgttg ctgtgttgca cattgtaaac tctcttgcta
    11461 ttcctgtaac attatttaaa agctactcac tttatgctgg agtacaggat gcattagtgc
    11521 aatggtggta tggacacaat gcagttgcat tttttcttac aacaccttat ctgggattga
    11581 tgtattattt tcttcctaaa gctgctaacc gtcccgtata ttcctatcgt ctttcaatca
    11641 ttcacttttg gtcattaata tttctttata tatgggcagg accacaccac ttgctttata
    11701 ctgcattgcc cgattgggca caatccttag gtgttgtgtt ttcaataatg ttacttgctc
    11761 catcatgggg tggtatgata aacggattgc tgacactgcg cggtgcatgg gacagagtac
    11821 gcgataatcc ggaacttaaa ttttatgttg ttgcagtaac tgcttatggt atggcaacat
    11881 tcgaagggcc tatgctttct ttaaaatctg tcaatgccat cagtcacttt accgattgga
    11941 caattgcaca cgtacacatt ggcgcacttg gatggaacgg atttctgact ttcggaattc
    12001 tttattggct gattccaaaa ttattcaaca caagtttata ttctaaaaaa ttagcgggaa
    12061 tacatttctg gcttgggaca cttggcattg tcttctatgc aattccactt tactgggcag
    12121 gtattacaca acatttcatg tggaaagaat ttactgaaga tggtgtgttg cgttatccaa
    12181 atttccttga aacagtagtg caggtggtgc caatgtataa actacgtgct ttaggcggat
    12241 cactttatct gcttggttct ttggtgatgg tttataacct tattaaaact gcacgtgctg
    12301 gaaaaatggt tgctaatgaa gatgcagaag caccggcatt agaaaaaaat tatgttgctc
    12361 atgatgacca ctggcatcgt tggatagaac gcagacctgc tcagatgctt atcggaagtc
    12421 ttattgtaat tctcatagga ggaatggttg aaattattcc aacttttatg gtgaagtcaa
    12481 acgtaccaac aatagcaagc gttaaaccat acacaccact tgaattacag ggacgtgata
    12541 tttatgtgcg agaaggatgt tatacatgcc actcacagat ggtgcgaccg ttccgctatg
    12601 aaactgaacg ctatggtgaa tattccaagc ctggtgaatt tgtctatgat catccgtttc
    12661 aatggggtag taagcgaacc ggccctgacc tgcatcgtga aggagggaaa tatccaaact
    12721 catggcacta caatcacatg ctcgatccgc aaaccatgtc acccggaagt attatgccat
    12781 cctatgcatt tatgattgat gatgatttag ataccaccac tactgcagca aaaattcgtg
    12841 ctatgcaaaa gttaggtgtg ccctatcctg aaggttatga tgcacaggca aacaaagatc
    12901 tgatgaagca ggcaaacagt attgcatcta atcttgaaaa agatggaatc aaagtaatgg
    12961 gtaataaaga aattgtagct ttaatagcat acctgcaacg attaggtgtt gatatcaaag
    13021 cagaaaaaac tacagcctca gttaattaaa aataaagaag aatatgtata aagaagtatt
    13081 atcaacaata aaggatatca gtatctatcc tgtattttcc ttcgttattt tctttgtttt
    13141 ttttaccctt gttggtgttt gggttttgaa atcaaagaaa gaagactttg agtctataag
    13201 taagctgcca ctagaagaaa caaacgatta aaatctgaag taatgaaaaa gataattaat
    13261 ttaatatgtt tgataatgtt catctcatta tctgcatggg ccggtgatac acaggcaaca
    13321 gaaacaacaa aagttgcagc cgctcagcct ccggattggt taatgaatgc aaatacttat
    13381 ctcattgcat ttgtagtaat tattttactt gccacaatat tggcattata tagaagcagt
    13441 cttaaactac tgaaaaaaat aacatcgttt tgctttataa tgtttgtatc aggttctgca
    13501 tttgcagcag aagaagcagc acctgcagaa gcaacaaagg ttacagctat acaacctccg
    13561 gactggctat tgcatacaaa tacttatctg attgcattta tcgtttttat tttagttgtg
    13621 acgatagttg tgttgttcaa aaccaacatg aaacttatca ggatgattgc accacatgca
    13681 tttgatgatg agaagcaaac agcagaagca ggagcagttt caggagtgaa aaaccgtgaa
    13741 acatttatgc gtaaagtata tctgcgctta gtggatagcg ttcctgttgc taaagaacag
    13801 gatattcttt tagatcatga ttatgacggc attcaggaac ttgacaacaa cctgccgcca
    13861 tggtggaaat ggggatttta tataaccatt attgctgctg tgatttatat gttggcatat
    13921 catgttaccg gcacaggaaa attgcaagcc gaagaatatg cagatgaact tgctgttgca
    13981 gcaaaacaga aggaagaacg aatgaaaaat tcagcagaga atattacaga agaaaatgtg
    14041 actgtgttgg ttgatgcagc agaaattgca aagggaaaag atatttatga aaagaattgc
    14101 tcagcttgtc acatggctga tggtggcgga atggttggac caaaccttac ggatagttat
    14161 tggctgcatg gcggaggcgt aaagaatatt tttaaaacca ttacttatgg tgttcctgca
    14221 aagggaatga ttagctggca ggcacaactt tcaccaaaac aaattcagga agttacctct
    14281 tttattatga cacttcatgg gacaaagcct gctgcgccta aagagccaca gggtgatgag
    14341 tggaaagatg aacctgctgc aggtgcaact gcaacagaca gtgctgctgt tgcaactact
    14401 gcagaggcag taaaaaaata gtattaaatc tcttttttat ggtggagaat aaaatggagc
    14461 atattgatga gtcgttcaga gacagcatag ccactgttac tgaagatggc aagcgtgcat
    14521 ggatttatcc taaaaaaccc aaaggcagat attataatgc acgaacatgg gtaagcattg
    14581 tgtttctgat tattttcttt gcattaccgt tgataaagat taatggccat ccattttttc
    14641 tgctcaatgt tgtagagctc aagtttgtat tgttcggaat tgcttttttt ccacaggact
    14701 tttatttgtt tgtattggcc atgctcacct ttgtggtgtt tattgcattg tttacagctt
    14761 tgttcggaag gctttggtgt ggatggactt gccctcaaac tgtttttatg gaaatggtgt
    14821 ttcgcaaaat cgagtattta atcgaaggcg attttatgaa acagaaagca ctcgataaac
    14881 gagcctggga tgcagaaaag atcagaatca agacaacaaa acatcttgta ttctttttca
    14941 ttgcctttct tatctccaat actttcctga tgtatattat cggatttgat gaatggaaaa
    15001 aaatcattac cgataatcct tttgaccaca ttggcggatt gtttgcaaca gtaatttttg
    15061 caggagtatt ttatggcgtg tatgcacgct ttcgtgaaca ggtttgtatt gttgtttgcc
    15121 cttatggccg tttacagggt gtaatgcttg accccaacac tgtagtaatt gcttatgact
    15181 ataaaagagg tgaaccaaga ggaaaactta gcaaagacaa tgaagaacat ggcgattgta
    15241 ttgactgcaa tcaatgtgtt caggtttgcc ctacaggtat tgatatccgc aatggcacac
    15301 agcttgaatg tgtaaactgc actgcatgta tggatgcctg tgatgacatt atggataaaa
    15361 tccagaagcc acgtggactc atcagatata cgagtgagaa tgctattaag aataatgcta
    15421 agtttaaaat caattcgcgc attatcggct attgtgtggt gctgttagtt ttgttctcgg
    15481 cacttgtagt tttattagcc agacgatcaa atattgaaac aacaatttta cgtgcatcgg
    15541 gattattgta tcaaaaaccg gatgaaagcc atatcagcaa tctttataac attgccttga
    15601 taaataaaac ttttggtaat atgccaattg aattaaaaat tgttgaacca aagggagaaa
    15661 tcaaatgggt tggtaatggt attacacatc ttaaagaaca atctgtggcg cagagtactt
    15721 tctttctgat tttgcctaag tctgaaatta aaaagacaaa aacaaaagta gtttttgaaa
    15781 tctggtcaga aggagaaaaa atagatcgct atgaaacttc attcttaggc ccaaataatt
    15841 aattgaaatg aaattcaact ggggacatgg tattgcattg gttttaataa tttttgcctc
    15901 atcaatattg gtatttgttt atgttgcttc caaacaaaaa aacgaattgg tgacagataa
    15961 atactacgag aaagcagtgg cttatcagca aagaattgat gcagaaaaaa atgcattaaa
    16021 cgaaaatgct gatgttaagt ataatattaa ttccggtcag ctaatgttga cattaggcag
    16081 aaatatacca aatgcaaatg gtactattca gttttataag cctgatgatg caacaaagga
    16141 ttttaatact tcatttcagg ttaacgatac aggattttat agtgccacta ttcctcaatt
    16201 aactcatggt caatggaaag ttaatgttag ctggaatgtt gacagtaaag agtattttta
    16261 cgagaagaaa atatttattc cataatgaac gaatacactt tctattatgc agctttgcta
    16321 ctgggttttc ttggtagctt tcactgtgta ggaatgtgtg gccctattgc cttaatgctt
    16381 ccggcaaata agttagataa tcgcagagct gcattttttg gcagactgtt ttacaacatc
    16441 ggcagaatga taacctatat gtttattgga atgttgtttg gattgctggg tgctgctttt
    16501 gttttaaatg gatttcagaa acaactgagc attattacag gaattatttt gttgttagtt
    16561 gcaatattta ctttaatccc ttcaaccaga aaaaaagttc ttagtgtatc atcaaaaatg
    16621 aatattcctt tcagaggagt gttaagaaaa cttttttcag gtaaaacagc cttgcatcat
    16681 tttggtattg gtgtcattaa tgggtttctt ccatgtggat ttgtatatgt ggcaacggca
    16741 ggagccatca attcaggcag tgttttaaac agtgttctgt atatggcatt gtttggtttg
    16801 ggaacttttc caattatgct tcttgtttcc tatgcagcat cgtggtgtag tttttcattc
    16861 tctcgttatg taaccaaagc cacaccttgg atatctgctg cattagcatt gtttcttatt
    16921 tacagaggag cttttatgaa tattaatgcc gattgtcata aacattcaca tccaccaaca
    16981 atggtagaat gtagtaagcc tgaaacactt taaaatttta ttttatagga ttgttcaatt
    17041 gccgatattc ggtggcttaa tgatatttgt atattcgcaa cgctacatta aatcgaaaag
    17101 gtaaatgagt cataaattat tagatgcctt gatgcacctt tttgccattc tggcaaatgc
    17161 cgaaaaattt tctctcgaag gcagaagtat agttgagtct tttttgcgac agcaaatcag
    17221 ccgtgcttat gttttaaagt accttggatt gtttgacgag tatcttgaaa atatcagagg
    17281 taaggcaaca gaaggaaagt tgaaaaaacg actttcagta aactcggtta aagtgctgcg
    17341 catttgtaca gacattaatg cagaacttga tacccgccaa aagcatattg ttttaatgcg
    17401 gctgattgaa ttcgttttta cctctgaaaa aattattgac agtcaggaaa aagagtttct
    17461 ttatacagtt gctgaggttt ttcatattgc acccgatgat gttcagttat gcattgaatt
    17521 ggcttgtaat aatgcagaag agaagtattc agatactcct aaatatttag tgttgtcttc
    17581 acaagatgat aacagcagtt tgaatactaa aactcaaatt atatcagggt taaatggtgt
    17641 tttccggttt ttatttctta aaaatgccgg attgcttttt gtgcgatatc acggtgatga
    17701 atcttttaca ttgaatggac taccactcaa tgacgattta tgttatgttt tttctgcagg
    17761 ctcagtcatc agaggttcaa aggtaaacac aatctattac acagatatta tcagactgtt
    17821 ttcaaatttt aagcaacaga gtttattgca ttttgatgta caacatgtgt attacgattt
    17881 ttcaaatggt aaacgcgggc ttaatgatgt tgcctttagt gcaacatcag gaaatcttgt
    17941 tggtattatg ggtggaagtg gcgcaggaaa atctacatta ctcaatatct taaatggaaa
    18001 caatagacca acacaaggaa aagttactgt taatggaata gatctgcatc gggagtcaca
    18061 tctgcttgag ggtatgatag gctatgtgcc gcaagacgat ttgctgatag aagagttaac
    18121 agtctatcag aatttatttt ataatgcaaa actttgtttt ggaaatttaa ccgatagtga
    18181 aattgatgaa cgtgtaaagg cacttttact aaatgtcggc ttaagcgaag caagtcatct
    18241 gcgtgttggc aatgcactca gcacatctat cagtggtggt cagcgcaagc gactcaacat
    18301 tgcattagag ttaataagag cacctgaagt actttttgtt gatgaaccca cttcaggact
    18361 ttcttctctc gactcagaaa atgtgatgga cttgttaaag caacttgcca atcacggcaa
    18421 acttgttttt gtagttatac atcagccatc gagcgatatt tttaaaatgt tcgataaact
    18481 ttttgttctc gatattggtg gctaccctgc atacttcggt aatcctgttg actcacttct
    18541 ttattttaaa caacgtgcat cttttgctga tgccgattat agcgaatgtg gcacatgcgg
    18601 caatattaac cctgaacaga ttttttcaat tctggaatcg aaagtaatgg atgagtttgg
    18661 ctttcctaca caacaacgaa agctaactcc tgcagattgg tatcaactgt ttaaggaaca
    18721 taatagtaat tctagtaaat cgaatcagcg acaacccgtt cagggtagtg caaatcaaaa
    18781 gcccggttgg ttgcagcaac taaaggttta tatgacacgc gatatgcttt ctaagtttaa
    18841 aaataagcag tacatgctta ttacttttct tgaagcaccg gtgctggcca ttattcttgc
    18901 atggtttctc aggtatttta atcctgatgg cagttatgtt tttagcgaga ataaaaacct
    18961 tgtagcattt ttattcatgg cagttatagt tgccattttt atgggactca ctgtaagtgc
    19021 cgaggagatt atccgcgaca gaaagatgtt gaagcgcgaa aagtttctta atctcagcag
    19081 aagcagttat ctgtattcaa agatgatggt gatgttttta atttctgcta tacagacatt
    19141 ttcttttgtc attatcggta atcttatttt tggaattcaa agcatgacct ttgattattg
    19201 gataatgctt ttctccgttt cgtgctttgc caatatgctt ggcctgaatg tttcatccat
    19261 gttcgacagt gctgttacaa tttatatcct cattccattt ctgattattc cgcaaattat
    19321 tttaagtggt gtgatggtaa agtttgaaga cctgaacccg cacctgactt cgcaaagaca
    19381 tgtgccgtta ataggtgaat tgatggcttc gcgatgggct tttgaagcat tagcagtaaa
    19441 tcaattcagt aataatcctt atcaaaaata tttctttaat gtggacagac aaatgagcga
    19501 agctacttac aagaaagatt tctggctgca gaagttgaat gatattgccg aaggtgtttc
    19561 taaaaacaaa tctaaaaatc cgaaagtaga tgttgagctt attactaatg aattgcacaa
    19621 agaagcttta gttaataaaa attttaaaac tgcaattgat tttacaaagg caaatgcatc
    19681 agaaattgca tcacaaatta aacctctgac agatatactt cgtaattact acatagaaaa
    19741 gtataacaat gcatctgctg ccaaggatag ttttgtaaag aaagaactga agcattcatc
    19801 tgtagcgatg ttgaacaagc aaaaagaaaa atacacgaat aatgctttga atgatcttgt
    19861 tttaaataaa aatgacttta ccattcttgt tcaggatggc aatagtattg tgcagcgatt
    19921 tcagcctgtc tatatgcaac ccgacccaac aacatggttt cggtctccat tgtatgttta
    19981 tgagaaaaat ttctttggaa tgaaaattca tacatggtat gtaaaccttt ttataatttg
    20041 gttgatgagc tttatactga tgacaacact ctatttcgat agtttcagaa aactgattca
    20101 ttgggtggga cacagtagca aacgatgatt ttttcttaac tgttttttca gaatattgat
    20161 ataaaattaa atctctgaag gagacaataa aaaactgcta ttaaatcatt tttttgttga
    20221 atgatttaat agcagtgcaa ttttaattac tattttatta agaaagaagt gttatccgca
    20281 cttactgtgt ccgcagcttt tgcatttaat acaaccctct tcaaaataca atccttcctt
    20341 gtcgccacac tctgcacaca ccttatcatt aacttttgta ccatcaggaa taaattgttt
    20401 caatgcacgc attacaccat ttttccacgt gttaatgctg tcggaataaa gatgcaaatt
    20461 ttccaccagg ttaactactt gcggtatcgg cattccatgt cgcaatacac cgctgataag
    20521 tttggcatag ttccagaatt ctttatcgaa cgagcgcgat agcccttcaa tagttacctt
    20581 aaatccttct ttgtcattaa actgaaaatc gtatctcgat ttgccatgtc catttttact
    20641 cttgataatc catcctgaag aaacccatgc aggaatactg aatgcatctt ctgccttacc
    20701 ggtaaaaatt tcataaggtt tattgtctaa tataccgatg tatgcaatcc atttttcatc
    20761 tgcattcata aagcgtataa cttttgcagc aagaatttca ggtcgttttg gtgcactcga
    20821 ttctgttatt acaggttcag cttttttctc ttcttttttt accaacactc cactgcgcga
    20881 accttctctg taaacagtaa taccttttaa tccggcactc catgcctgca tataaatttc
    20941 gccaacagtt tctgctgaaa cattttcagg taaattaata gttgagctga tagagtgtgt
    21001 agtatatttc tgtactactg cctgcatttt aattctcttt ttccagtcaa tttcattggc
    21061 tgtgcttcct gcataaggac ttttactgta gtcggtgctg ttgtttgcag tcatccattt
    21121 ttttaatccg tgatgataca cttcaaactc ctgccaggca tcaccggact gatctacaaa
    21181 acttgtttta gccttgctgt cttcggcatt aacttttctt ctgcgtttgt aactgagcat
    21241 aaacacaggc tcaatacccg atgatgtttg tgataacaga cttaatgttc ctgtaggagc
    21301 aactgtgctg atagaaatat ttcttcttcc gtacttcatc attctgttgt aaatatccgg
    21361 caattctgct ttcatcatat ttacaaactc cgactgatct tctattgcag tatcaaaacc
    21421 ttcaaacgtt ccacgttcta tggccatatc aatacttgaa tcaaattctg cacgacattt
    21481 ttctttcatc atcatttcta caatgctaag tgcattgtcg gagtcaaagg caatgttcat
    21541 ggcagcaagt gcatcggcta aagctgtaaa tccaagtcct gtacgtcttc cttttttacc
    21601 tgcttcatac aatgttatcc atgtacgcat ttctacttct ttaatgtagt caggctcagg
    21661 gtctgattct attttctgaa gaatacgttc aatagcctct aactctaaat ctaccaaacc
    21721 atccatgagt ctttgtgact cataagttac ttcataaaaa cgtgtgaagt taaacttagc
    21781 cttttgtgta aatggatgat ctacaaaact caataagttg attgcaatta atctgcaact
    21841 gtcgccaccc tgcattgcaa tttcagagca agggtttgtt gacacgtttt taaatcccgg
    21901 ataaacggaa gatgtagaat aatgatgttg tctgtcccag aaaataattc ccggttcggc
    21961 agtacgatgc gcacaggcaa tgatggtttt ccataattcg cgggcattaa cttttttctg
    22021 aatataagga tcagtggaat caacagggaa tctcaggtta aaatcagtat tctcattaac
    22081 ggcaaccata aactcatccg ataatttcac tgaaatattg gcacctgtaa ctttactcag
    22141 gtcttgtttt acggtaatga atttttctac atcaggatga gcaatatcta gtgtaagcat
    22201 caaggcacct ctgcgtccga gttgagcaac ttcgcgcgtg gtgtttgaaa aacgctccat
    22261 aaaagataca gcacctgtag tagtacctgc tgcattggaa acacgcatac cttcaggtct
    22321 tatagtagaa aggtctaagc ccgcaccaca tctgcgctta tataattgtg caagttgctg
    22381 gtcggcaaaa aatattccac cataagaatc gtgtaattca ggaagaacaa tacaatttga
    22441 taatgatgca atcataaaag gattgcccag agcagacatt acactgcctt gtggaataac
    22501 ataacgaaag cgatcgaata acgctgtgat tttttcagta gttagttgct cgcgtttttt
    22561 tccgtattcc gacagatgcg aagttctgct gccatcgttc agcagattat attttgcttc
    22621 aactcttgca aattcacgag ccatacgcaa atgcatatca tgtggtgttc gttctacaaa
    22681 tttcccttct ttgtttttca tggcgtattt attcatccat gttgtagcag caagctcatc
    22741 gccttcaaaa tattctagac ttgctttcaa tacgctgtca tattcgtagg tggtcatctg
    22801 actctcggtt gtaatggttt caatcaacat attttatatt tcttatttgt ttaaggcaag
    22861 caataatata gacaaaaaaa attctttcat aaggatttga ctaatattta tcagacgttg
    22921 ttaacaaccc atgttcacta tttttattat caacaaagta catcaaacaa agcgtttgaa
    22981 ttataggcaa ttggtacttt tgctaacaca caagtaaaaa acaaatgatt ataatcatta
    23041 gcaaaattta atctaatcat taagcaatag aaaatctatt tggttcttcg catctttcaa
    23101 attctaatat gaaattcaag acaattatcg agcctttcag aattaaaatg gtagaaccca
    23161 ttcacctcag cacagaggaa caaagagtag gttatctgaa gcaggcacat tacaatccat
    23221 ttctacttaa cagcaatcag gtacttatag attttctgac tgacagtggt acttctgcca
    23281 tgagttctga ccaatggtcg gcaatgatga atggcgatga atcgtatgca ggtgcaagta
    23341 gttggctgcg ttttgaaaaa gaagtgcaaa gccttacggg aatgcctttt gtgttaccta
    23401 cacatcaggg tagggcagca gagagaattc tttatggaaa gcttggtggc aagggtaaaa
    23461 cgtttatcag taatacacac tttgatacta cacgtgccaa tattgagttt agcggtgccg
    23521 aagcaataga cattcctgtg aaggaagggc ttgactttgc atcagatttt ccttttaaag
    23581 gaaatatgga tatagtttta cttgaaaaaa ctattgcaga aaaaggtgca gctaatattg
    23641 gtgcagtaat attaaccgtt accaacaata gtggaggagg acaacctgtg agcatggaga
    23701 atgccaaggc tgttgcagat atttgtcgta aaaacaaagt gttgtttgtt attgactgct
    23761 gccgtgttgc tgagaacagt tattatattc atcataacga agcgggatat gaaaataaaa
    23821 cctacagaca gattgcagaa gaaatgatgt gtttgggtga tgctgctgtt atgagtgcaa
    23881 agaaagatgc attagtaaat atgggtggat ttttagcagt gaaagatgct gatttggctg
    23941 aatcgtgtaa acaattgttg attataactg aaggttttgc cacctatggc ggacttgccg
    24001 gacgcgatat ggaagccata gcagttggat tgcaggaagt gtttgatccg ggttatttga
    24061 attatcgtat tgtaagcaca agatatttgg gcgagaatat taaatccaaa ggagtgaata
    24121 ttatttatcc tgtcggtggg catgctgttt atgttgatgc caaaagttta tatcctcaca
    24181 taccaccaca tgaatatccg ggacaagcct tggtatgtga attgtaccgt attgggggtt
    24241 tacgcagtgt tgaaatagga tctgtaatgt tcggaaagta tgattcaaat ggtaagctga
    24301 ttccggcacc tatggaattg gtgcggcttg ctgttccaag aagagtttat acacaaagtc
    24361 acatagacta tgtgattgaa atttttgatg aaatagtgcg tacaaaagat aaggttaaag
    24421 gactaaagat tgttaaagag ccacaattcc tacggcattt tacagcgcat tttgagccac
    24481 tgtaattttt gtgattagca attgaacatt gcttaatgcg gaaagcagca ttatcgaaaa
    24541 gttgaagttg taaatagcat ttaccagcca ccggtggcgc caccacctcc ggtgctgccg
    24601 ccaccgaagc ctccaaatcc tcctccgcta ctgccaccgc cccaactgct gccaccgcct
    24661 ccccaaactc caccacctcc tgatgagtta cgtctgttac tcgctgcatt caaaagcatc
    24721 caggctgtcc agaaacttaa attgttgagc tgagcatagc gcctgacaga aaaaactttc
    24781 atcagcatta ctacaccgaa aattaaaaac acaaaaagta tgggtgccca tgcaggtgaa
    24841 ttgttctttg catttttgcc atcagaaaca tattcgcctt ttgttcgctg cataatttct
    24901 gttactgcag catcaagtcc gttgtaatac tgttgtgatt tgaatgcagg cttcagcgta
    24961 agctcaataa ttcttttaca aatagcgtca ggtaatgctc cttcaacacc gtaacctgta
    25021 atgatggtca tgcgcctgtc ttccatagca gctaaaatca gcacaccatt atctttattc
    25081 ttctgaccaa ttttccaatc tctgaaaaga gctatagcat aatcatccac aggatattca
    25141 ccggtactgc gaatcagtac aatagcaatt tgtgtagaag tgctgtcgtt aaattgtaca
    25201 agtttttgct ctaaaagatt ttcatcagca tcattcaaaa ctcctgcata gtcgttgact
    25261 aagcgtggtg gattactttg aggtggaatt tgttgtgcat taccaattgt tgcaatctga
    25321 agcaacaata acatccataa tataagagga cgtacctgtt ttttaatcgc taaaaaccac
    25381 ttcattggaa agttcgtctg aatcattctt agcaacagga aaatactttt ttaatttctg
    25441 acctgtttct tcgatagcat gcaacaagcc accggtataa tcttttctct taaagaatgg
    25501 aatcatttcc tctttaacct tatcccaaaa aacctgactt acatattcgt gaataccact
    25561 atcaccaata atggcaaact gtctgttttc aatggctaca taaataagca cgccattgtg
    25621 aagtttggtt tcatgcatct tcaggttttc aaaaacttcg gctgcacggt caataatttc
    25681 actctttacc ttgctgtcaa taaaaattct gatttctccg gatgtatgtt tttctgctct
    25741 tgctattgca tttttcaaca acaattgttg ttcatcagaa aaataatcgg cagcgttggc
    25801 cataattaaa acttaacctc aggggcttta ttagcgcctt tatcagattg aaaataacct
    25861 tttggtgaaa acccaaacat gccggcaagc aatgtttgag gaaacttacg tatgtaggtg
    25921 ttaaactgtt gtgcagcctc gttaaaatct ttacgtgcca cagcaattct tctttctgtt
    25981 ccttcaagtg ttgcctgcaa ttcaagaaag ttctgattgg ctttcagtac cggataattt
    26041 tcagtcacca taagtaagcg acctaatgct gtgcttaact gcccttgtgc ttcctgaaac
    26101 ttctgaatat tttcaggtgt aagtttgtca ggatcaactt ttacttgtgt tgccgatgca
    26161 cgtgcttcaa caactgctgt taaagtgcct ttttcaaatt ctgcagcacc tttaactgta
    26221 tttactaagt ttggaataag gtccattcgt tgttggtatt ggttctctac ctgtgcccat
    26281 tttcctttaa cattttcatc ggcagtcacc atgctgttgt aggtgcctgc aaacaatcgg
    26341 tatattataa atgcaacaac aagtaaaata ccaatgatta ctactgaacg tttcatacta
    26401 tatgatttat ggtttataag ttaacgtgcc aaaactaaaa tgtttgataa ttcgcttttt
    26461 gtctaatagg taaaagtttt aaaattgtag gtgaatagta ttattttgcg gataagctaa
    26521 aaatctttga agcaaacaaa tttcttaaca ttgcaattca ttgtttaata gctgaatgat
    26581 aaagactgta gcaattgaaa ttacaggtaa agtgcaaggt gtgtttttta gaaaatttac
    26641 acaacagaca ggtcgtagat tgaacctttg cggttttgtt cgtaaccgct ctgacggttc
    26701 ggtttatgtt gaagcaacag gcagcgaatt gcagttaaac gaattggtag catggtgtca
    26761 taaaggctca cccatgtctt tagtcaggca ggttgttgtc agagaaattt ctgtcattca
    26821 caatgaacca tttcaaattc tgtagtaagt gaaagtaaat tataaaacgg ctactttagc
    26881 cgattcatga gtggaataaa aaagttagcc ggtcaaacgg tactttatgg tctgagtagc
    26941 attgttgggc gtatgcttaa ttatttactc gttcctattt atacccgcat tttttctccg
    27001 gcagaatatg gtgtggtttc cgagctttat tcctattcag catttcttct gatactgttt
    27061 acctacggca tggaaacagc tttttttaat ttcatgcaga aagaagagaa taaaaataag
    27121 gtttatgaaa attgtatttc attagtttct tatacaacaa caattctggt gttgctgatg
    27181 atatttttct catcgccatt ggctgcaact attgagtatc cnnnnnnnnn nnnnnnnnnn
    27241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27301 nnnnnnnnnn nnnnnnnnnn ntcaaacgtt atagccagtg gttttaccat gttattactc
    27361 taccggcaat tcagtaatct aacctggcac ctcgacagcg ctttaataaa acgaatgatt
    27421 tattatgcat ttcctttact tattgcagga tttgcaggta tggttaacga aacattcgac
    27481 cgtgctgttt ataaatattt agctccggat gccaatcatg ctttgcgcga tttaggaatt
    27541 tacagcgctt gttacaaact tagcatcatc atgacattgt ttgtgcagac atttcgatat
    27601 gctgccgagc catttttctt tgcgcagcac gataaagaag acaaaaaaga aacttacgct
    27661 aaagtaatga catggtttgt tatcacatgc tcatttattt ttttaggtgt aatgttattt
    27721 attgacgtca ttaaactttt tattggcgaa caattcaggt cgggtattga tgtggttcct
    27781 gtgctcttgc tggcgaatct ttgtcttggt gtattttaca atttaagtat gtggtataaa
    27841 ctcacagaaa aaacccgcta cggagcctat ttttcaattt tcggagcagt gttgactatt
    27901 attctattgt ttggtcttat accttcgttt ggatatatgg gtgcagcatg ggcaacattc
    27961 ataacttatt ttgcaatgat gattgtatct ttcatcaccg gacagaaaca ttatcctatt
    28021 ccctataaag ttaagaacga tgtgttttat gttttgctat ctctaatgct gttttcactt
    28081 tctgcctatc tgcaacatca acttgattta cctggttctg ttaaatggat tgtaaatata
    28141 attttactct ttgtttttcc gtttgttgta ttcagaatga ataaagatct gctggtaaaa
    28201 aagcaaaaag tagaatcaca acagaataca attggaagaa gaaactgagt gtatatttga
    28261 caagaatcaa tatttttgca aatagcgaaa gaattaaaaa tgcaaaacaa aacagaagta
    28321 aagattataa ataaatcatc gcatccgctt cctgcttatg aaagcaacag tgctgcaggc
    28381 atggacctta gagctgatct gaaagaagcc gtagaactta agccattaga acgtgcttta
    28441 ataccaacag gaatttatat tgaattacct caaggttttg aagcacaaat acgcccaaga
    28501 agtggacttg cagcaaagca tggtgttacc gtattaaaca ctccgggtac tattgatgcc
    28561 gactatcgtg gagagataaa agttattttg gtaaatctgt catcggtggc atttacaata
    28621 aataatggcg aaagaatagc gcaaatggtt gtggcagcac atcaacaagt agtgtggaaa
    28681 gaaacactgg agttgtcaga aaccaatcgt ggtgctggtg gttttggcag tacaggtaaa
    28741 taattgatga atagttttat caattaacat attgttagaa ttactcattt cagagaataa
    28801 tagttttgaa ctcaaataaa attgtgtgat cgaatcatta gttatcatac cgacatataa
    28861 cgaaaaagaa aatattcaga agattattaa agctgttttt aatctgttga atacttttca
    28921 tgtattggtt attgatgaca attcatcaga cggtactgcc gatttggtga aacaacttat
    28981 gccacagttt cctgattcat tacatttgat tcagcgacaa ggaaaaatgg gtttaggcac
    29041 tgcatatatt catggtttca agtgggcatt agagcgcaac tatcagtata tttttgaaat
    29101 ggatgccgat ttctctcaca atcctgccga cttgcaacga ttgctcgatg cctgtaaaaa
    29161 tggtggtgcc gatgtggcag taggttcaag atatatcaat ggtgtgaatg ttgtaaactg
    29221 gcctattggc agagttatca tgtcatacta tgcaagtgtt tatgtgcgac tgattacacg
    29281 catgcctgtt cgtgatgcca cagcaggatt taaatgttac aggcgtaaag tgcttgaaac
    29341 actggatttg tcaaagatta aatttatggg atatgcattt cagatagaaa tgaaatttgc
    29401 tgcctggtgc agcggcttcc gaattatcga agttcctata atttttaccg atcgaacaga
    29461 aggtaaatca aaaatgagca gcggtatttt taaagaagct ttctttggcg ttattcagat
    29521 gaaaataaaa agctggtttc gtaaatacac acattaataa gatcatttat attatttatt
    29581 gcgaattgaa aggaatatga tgttcaaaat aaggcgtgtc caaacagtaa aataaatttt
    29641 ataaagagtc aagacgaaat attatcttgt aacgaacgct gagggtactc ttttatcagt
    29701 attatatcag gttctgctgc ataagaaaag gctatctcat aaattgaaat agccttttta
    29761 cttttttaca atttggaatt ttaggctaat cttacattta ctgcatttaa accttttttt
    29821 ccttgttgta cttcgtaaac aacattgtcg ttttcgcgaa tttgttcttt taaacctgaa
    29881 gagtgaacaa aaatttcttc tccgttttct gctttaatga agccaaaacc tttggtttca
    29941 ttaaagaact ttactgtacc attttgcatt gtttgttgaa tttaatttta ctttagtttg
    30001 aattatttat atggatgtgt ttttacaaca tcaatgtttt gtttgattaa tttattgata
    30061 tcattcagat atggcttttc atcatctgtg caaaaggata aagctatacc cgattcaccg
    30121 gcacgacctg tgcggccaat acgatgaaca taagtttcgg cctgttcagg aatttcaaaa
    30181 ttaataacat ggatcagtct gtccacatca ataccacgtg atgcaatatc tgttgctacc
    30241 aaaatttgta ttgatctttt tttgaaacca ttcaaagccc gctctcttgc gccttgagac
    30301 ttgtcgccat gaatagcctc tgcttttata ccgtttttat ttaaatcttt taccaccctg
    30361 tctgcaccac gttttgttct tgtaaaaact aaggcatgat taattttatt ggactctaat
    30421 acatgacgta acaaggagcg tttattttct ttttttacat gatagactaa ctgctttacc
    30481 tgttcagccg gtgtagaaat aggagtaaca ttgaccttag ccggattttt caacaatgtg
    30541 ttggctaatt gcatgatttc cggtgccgca gttgcagaga aaaacaaagt ttgcttttgt
    30601 tgaggaagct ttgcaattat cttttttata tcattgataa atcccatgtc taacattctg
    30661 tctgcttcat ctaaaacaaa atactctata tgttttaaat ctatgaaccc ctgattcatt
    30721 aaatcgagca aacggccggg agttgcaatt aatatatcaa ctccattttt taaggcattt
    30781 gtttggttta cttgagatac tcctccaaaa agtacaacat ttctgtaagg gagtttacga
    30841 ccatattcgg aaaaactttg accgatttgt aaggcaagtt cacgagttgg tgctagcacc
    30901 aaagccttga tgtgattgcc tggcttatgc tttttaaggg aaatatgttg aagaataggt
    30961 aaggcaaagg ctgctgtttt tcctgtacct gtttgggcgc agccaaaaat gtcgcgaccg
    31021 gctaatacta ttgggatgga ttgttcctga acaggagttg gcttactata tttcaactcg
    31081 tttaagactt ccaatagggg agtaatgaga tttaaatttg aaaatgacat taaataatat
    31141 gggattttta tctgacggat ttaagggttc atgtagagtt atttaccctc ttcttaaaga
    31201 gaagttaatc cgtagttaac ttgatttgct tactataata taactgtttt gtaaatagtt
    31261 tgcaaagtaa caacaaattt cttaaaaaag ctaagaatga ttaaatttag tttgagaatc
    31321 gcatcattta tcgagtaaag ttaggcataa atgctaaaaa ttaacctaaa ttgatggtga
    31381 cggattgatg ttcgcttaaa ctaaatgaga tattatatca aacaaacttt tcgcctttag
    31441 cactctttac atcattcaca aactgacgta tttgttgttc gtcctgcttt ctgcaaacca
    31501 ataaaatatt ttctgcttca acaacaatgt aatcggacaa gccttgtaga acaaccagtt
    31561 tatcatgtgg catatttaca atgcagtttt tagtatcgta caacatcaca tttttcccaa
    31621 ccactgcatt tttattagca tcgtgtgtta tgtgctcata aagtgaacca taagtaccta
    31681 aatcactcca tccaaaatca gcactcatca caaaaacatt tttcgctttc tccattacac
    31741 caaagtcaat gctgatgttg gtacattgcg aataggctgt tttaataaat gcatcttcac
    31801 ctttgctgtt gtaaatggaa ttaccttctt caaaaagatg atacatctcg ggcagatgtt
    31861 cttttaaggc ttttaatatt gacttaaggt tccataaaaa tattcctgca ttccagacaa
    31921 actcacccga ttgcagaaaa aagcgagcca tttccagatt tggcttttca gtaaaagttt
    31981 taaccttgaa tattttattt atttcattgt gtttttcatc tacaaactga atgtagccat
    32041 agcctgtatc cggtctgctt ggtgtaatac ccaaggtaag caggcagtca ttctgtgcag
    32101 tgaaagtaag acctttgtta atggcctcaa taaaagcatc ttcctttgtg atgatatggt
    32161 ctgacggggc gacaactata ttggcatcag gattttttgc agcaatttta aatgcagcat
    32221 acgaaacaca tggtgctgta tttcgtctga gtggctcgcc cagaatattt tctaccggta
    32281 gttgtggcag atttttcttt actaaatcaa tatacgattc attggttaca atatagatat
    32341 tttctatagg acaaatttta gtaaaacgat taaacgtttg ttgtaaaagt gtttgtcctg
    32401 ttcctaaaat gtcaagaaat tgtttcggaa aagcatttct gctcattggc caaaaacggc
    32461 ttcctatgcc tccggccatt attacgcagt aattgttttt gttcattatt tttaaaagat
    32521 tgtcttaaat tttatttgat agattttaaa cgcaaaaaaa tgaaattaaa gtctggcaga
    32581 atgaataatc gctttttttt attaacgaat agatttgata attatttgtg ttttggcttt
    32641 tcagtacaat atagtgttaa ataaaaaacc ggctgatagt atcaaccggt tttataaatc
    32701 attgagttat ccttatctgg aaataacttt caaacgctga acagattttt ctccttcaac
    32761 ttttataaaa taaacacctt tggaaaaatt gctgatatca acagcatgac ttttgttgtt
    32821 agccgggaat acatcttcag ctataacttt tccgttaatg tcataaacag taagtgtata
    32881 tgactcatgt gatgtttgct tactgtaaat aataaatttc ccgtctgatg gattcggaga
    32941 aatatttacc gttccaacat tattgtccaa ttgatttaat cctgtagtaa catcaagatt
    33001 tacattgtca acgtacatag agttgccaaa atcgttgatg tttacaaatt ttatcgaaac
    33061 atcctgacca aggtatgcat tcaatgaaac agaatcattg cgccattgat tggcagcatt
    33121 tggagcaaag ttgctggttg tggtacccgt agtagcaaga gccactccgg tttttaagta
    33181 acctgaagga acccatgtat ttccgcagtc tgttgaaatg tcaactcgca aaccatcatt
    33241 ataaacactt gaatattgtg catacgctac atcaaatgtc agtaatgctg ctgctgcatt
    33301 ggataaactt acttcataac taattaaacc atcttctgat cctgaactat tatatgagta
    33361 attgttgaat cgtgcagcat aagtagaagt gcctgctgat cctgtaatga gagctgattt
    33421 taaccatgta taattagcgt ttgatgaatc aatagaccag tttgccggag ggaaaactgt
    33481 agcctgaaaa tcttgtgtca ttggtggtaa agaaggagca agaatattaa tcattgcagt
    33541 ttgtgttgaa cttgaagtgc caattccatt ggtagcatct aaagttacat cgtaagtgcc
    33601 gggtgtatta aactgtactt gtggattttg tgatgttgca gaagttccac ctacaaatgt
    33661 aacggtatta ggattaaatg tccaattcca acttgacggt gcattagagc tttgatctgt
    33721 aaaagtgaaa gttttgcttg tacaacctac attgaagttt gcattaaact gcacagatgg
    33781 aggaatgttt ggacaattga aaatgccaag ttgtttttta atggcattag ccctgcgccc
    33841 tttattgccg ttagccatca ctgcccttac gctaaaccaa taagagtttg atgctataag
    33901 gtttgtaaaa atataatcgg tgttggttgt tgtggcaaca gagtccatat acattgtacc
    33961 taacatactc acttcatacc aagcagcatt acttacggct gcccatccaa ttttaattga
    34021 atcaggacat gagtaaataa catttaaatt ttgtggaatg ccaacaatgg taaatgttgt
    34081 gtcagacaat ccagaaacac ttcctcttgt aacacgaaca cgggcatcac ctgttgttgc
    34141 aatattatta ggcacattcc acgaatattg taatgtattc tgatttacag tggcaatatt
    34201 attccatgta gttccattgt cagtagtata ttctacatca aatgagccaa gacctttcaa
    34261 tgcatcccaa cggataattt caggatgacc gggaacaaaa ccttcgccac ccataggata
    34321 ggtaacagtg atgtcatcat ttctgaactc ataaactaca taataagtct gtggtccttg
    34381 tggaatagca aatcctttga gtgaaacaac atagttacct gctgcagggt tgtctaatgt
    34441 aacttgttcc atgttgttca gatggtcaat gcctcttgtt gcaggtgcat tcagattagc
    34501 ggcattggga gtaggatcaa gaacccatgg attccatact gcatttgatg ggtcggttac
    34561 ttgcatgtca atgtcgttaa ctaaataagt agatgatgcc ggtgagccac ctacatcatg
    34621 ccagtaaacc atgacacgca tttgcgaagt acctgcagga acgttaatag aataagtcaa
    34681 tgagtcactc tgattaatac tgtcagtaaa ataacgtgca tcttcaatag tagtaacagc
    34741 acgcagtgca tttactcttc cccaaccata tttgtaatca gggccagggt ttccgatatc
    34801 ttcagcacca ttgagcaagc aagctttaat taaagcagta ggtgcatcgg tagcagaatt
    34861 aatttgctta taagcttgaa taagttgtgt gcagatacct gcaatgcccg ggcaggctgc
    34921 agaggtgcca ccaccaacct gataagtatt attttcatct gttgacattt ggtcgcggcc
    34981 attggcagca atatccggtt ttactctgcc gtcagaagcg ggaccaatac tgcttgaagg
    35041 atcaataacc tccaaagcat caagattggc tacagcaatt acattttttc cctgcttgta
    35101 acctcctgta atatttcccc aattacctcc ggcaccatag ccacaattgg taccattgtt
    35161 atttcctgca gagaaaacaa attcaagttg tccattgtca tgaatagtct gatcgccaaa
    35221 ttgtgtgtcg gtagtatatt cattacatcc ctgcgaatag ctggttgaag caaccactgt
    35281 tccaagattg ttatagttgg caactgcatc aataatttgt gggtagcttc caatatcaaa
    35341 aacatgcagt tgaactccat tggccatacc cggatatgca ggatttaaat tgcctgcacc
    35401 tgcaagaatt ccgcctgtca tgtcaccatg cgaaggacca actgttgttg tgtaattggt
    35461 aatccttccg gtaaaatcaa tgtgtgggcc aacaaatcca tcatcggcaa gtgctgcggc
    35521 tactccgctt ccattgtatt tacgtcccat caacaagtcg ttgttgatga cattgctgcg
    35581 gtgcagacta cgtccttcca aatcatcttt aacagatggt ggcgaggtta aatctataaa
    35641 aaacaaccaa ggctgttgtg aaatagtggc aacagattgt tgctgcatgc gcacctcaac
    35701 catattattt atttcagttg aacttaaaat tttgccatag gtagcggcaa tctgttgtgc
    35761 ttgttgtatt gaaatgtttt tctgaaactg aagtctcaca tcaaccattc ctgcagaagg
    35821 aacagcgtat ttaggataat cacctaacat gctgatagaa agtttctgtt cagaagattg
    35881 atttgttaca cttctgacat taaatcctga gagcaattgt ttgttaaaac taattggaat
    35941 tgctgcatta aaagcattct ttggcaagta ctccaacagc ttaattcctg cagtttccat
    36001 tacagctttt tctgcttgag taggtacagt ataaaactgt atgattcggt agtaataacc
    36061 attataaaca tcgttttctg atactttttc ctggataaaa gagtttaggt tttctgccgg
    36121 caaaacagct ttacctaaaa acatcaggcg atagtctgtt tgcgccatag ccattgttgt
    36181 agtacacaac aacataataa agagaaatcg ttttaacata gattgatttg attttatgaa
    36241 ttttaaataa agggtctaaa attacagaaa acattcgtta aatgaataat tgtttaataa
    36301 ttgattatca gctaaaaggg tttcttaaaa acacatcatt gtattagtgt tttggttact
    36361 tttgcacact tatgaaaaat cgtctgtcgg tttattttct tttaatggca ttgctgctga
    36421 ccttaggctc atgcagtaag tttaatcgca ttcgtaaaag tggcgatatg aacatgaagt
    36481 atgatgctgc agtaaaatat ttcgaaaaca aaaaatattt taatgcatta cagctttttg
    36541 aagagttaat agttgtgctt aaaggaaccg ataaggctga aagctcttat tactattatt
    36601 gtttaagcta tttctacacc ggtgattata ccagcgcagc ttatcacttt aacaattttg
    36661 cacaaacatt tccaatgagt gccaaagctg aagatgcttt gttttataat gcttattgct
    36721 attacttgga ctctccgcct tcaagcctcg atcagcagag tagcctcgat gccatcagac
    36781 agtttcagtt atttatcaat aaatatccaa caagccagcg tgttgccgaa tgtaataagt
    36841 atattgatga gctaagaagt aaactcgaag taaaggcctg ggataatgca atgctttact
    36901 acaaaatgga agagtataaa tcggctatcg ttgcctttga aaacgtggtg aaggacttcc
    36961 cgactaccaa atatcaggaa gaatgtttat ttttgtcgct taaatcggct tatctctatg
    37021 cccaaaacag catagaatcg aaaaaagaag agcgttacaa gagtactata gatcattaca
    37081 ataagctgat tgagatattt cctgcaacgg aatataaaaa agaagccagc cgtatattgt
    37141 cggcagccga agaaaaatta aaagattatc atactgtaaa ttgaattaaa acaatgaata
    37201 gcagaaacgt agcacaaaca acagtaaccc gcgatgtggt tgactttgaa aagaagactg
    37261 aaaacatcta tgaaagtctt gccattattg caaaacgtgc caatcatatt gctgccgaaa
    37321 cccgcgaaga attacacgat aaactaagcg agtttgcctc ttcaacagat actttggatg
    37381 aggtttttga aaaccgtgag cagattgaaa tctctaaata ttacgagcgt ctgccaaagc
    37441 caacattggt tgccattcag gactttttag atgataaaat ctatttccgt agtcctgcaa
    37501 aagaagaaaa caaagacagc gcagaaaatt aaaatcatgt tgcagggaaa aaaaatcctg
    37561 ctgggtgtta caggaagcat tgcagcttac aaaacacctg aattagtgcg acaattggtg
    37621 cagcaaggag caacagttaa agtaattgtt actgctgctg ctaaagattt tgtttcacct
    37681 cttgttcttt ctaccgtttc aaaaaacgaa acacttcaaa catttacttc agaagataat
    37741 tctacctgga ataaccatgt tgaattaggt ttatggtgcg atatttttct ggtagctcca
    37801 ctatcagcca acacacttgc aaagtttgcc aatggcattt gcgacaattt actgactgca
    37861 gtttttctgt cttgccgcaa tacagttatg gtagcacctg caatggatgt ggatatgttt
    37921 aaacatcctt caacacaaaa aaaccttgag attattgcca aaaacaatat taaaattatc
    37981 ggtccggaga gtggcgaatt agccagcgga cttacaggta aaggtcgcat gacagagcct
    38041 gaagatattg tgcaggaagt aatcaattat tttaatccat cgttaccttt aagaaacaaa
    38101 attgcagttg tcaccacagg cccaacacag gaagcattgg atccggttcg gtttattgga
    38161 aatcattcct cagggaaaat gggtgttgcc attgcaaact gtttagctgc taaaggcgca
    38221 tcagtaattc ttatctcagg cccgtcagtt agtttaggaa atttgaataa gtcaataaag
    38281 atagttcata taacttctgc ggccgaaatg ttgaaagcat gtaaacaggc acaacccgat
    38341 gccagcataa tggttatggc tgctgctgtt gcagatttta gacccgaagg catttctgat
    38401 caaaaaataa agaagaaaaa taatgagggg ttaagtgttc agttagtgcc aacagaggac
    38461 attgcagcag aaatgggtaa gctgaaaaaa gaaggtcaga ttttggtagg ctttgcctta
    38521 gaaacgaatg atgaaaagca gaatgccgag cgaaaactga aaaataaaaa cctcgatttt
    38581 atcgttctta ataagttgga agaatcgaat caggttttcg gttcagactt taaccgcatc
    38641 accatcattg ataaaaacct ggtgacaaca ccatttgaaa aaaaatcgaa agatgcagtt
    38701 gcatccgaca ttgttgagaa aataattact ttagtgcaat gaagaaattt ttctgtttag
    38761 taataatcat aatgagtttg catactgtgc atgcacaaga actcaattgc caggttaatg
    38821 taatggcacc acagattcag aacgtagata aaaaagtgtt tgaaacacta cagcagtcta
    38881 tttttgaatt tatgaataac accaaatgga cttctgataa atatcttaat caggaaagaa
    38941 tagaatgttc tgttacaata acaataaatg agcgtgtttc aaacgatgag tataaaggaa
    39001 gcattcagat aacttcaaga cgacctgttt ttcagacatc atataattca ccgactttaa
    39061 attttaatga cgataatttt cagtttaagt atatcgaata tcagcctcta gagttcagcg
    39121 agaatggaag caatccaaat ctgacagcag tacttgcttt ttatgcaaac attattcttg
    39181 gtgtggacta tgacagcttt tcattatatg gaggagcgcc ttattttgcc aaagcacaaa
    39241 ctattgtaaa caatatgcag aatgcacgcg aagcaggatg gcgtgctttt gaaggcacaa
    39301 aaaatcgcta ctggctctct gaaaatatga ctaatcctat ttttaaacct taccatgaaa
    39361 tgttttatca gtatcatctg aaaggccttg acgtgatggc aaaaaagaaa gatgatggat
    39421 tgtcgcaaat ttctgaaagc cttgatttgt taatgtccgt acataaagaa aagccgggtt
    39481 cgttgttatt acaaactttg ttttatgcta aagcagatga gattgtaaat atttattcag
    39541 gagcctatcc cgatcagaaa gtaaaagtca gcaatctgct aaacgaaatt gatcctgcaa
    39601 acggaaccaa gtactcgaag attatgggga attgattgtt gtagaattca aaaagtaaaa
    39661 atttttattc cttattacta ttatcaatta cttcatcaga aggtaaatcc acagagttgt
    39721 ctttgtattg ctttgtatag taaagatatt ttcctacgcc tgtgcaggca tagcctgata
    39781 ttgtatctcc gggtaaaagt tgtgttcttg taaaagtccg ccagcgtttt gatttttcct
    39841 gaacggctaa tttaacggca agggtatcgc tacacgtatt tacaaaccaa actttataag
    39901 gctttgttga tgcaggacat gataatgttt ctgcaccaaa ttttttattt actttttgta
    39961 agcactgccc gtaatctttt ccatcctgct gtattttgaa agcagataaa agcaccactg
    40021 caccaatcag tattaaaaaa acaaactcac gcaccttgat catttacagt aattattttt
    40081 aagaatgatt ttagcttctt cgtttcctaa tttttttgat ttgttccagt cttcgcaagc
    40141 acctttaatg tcaccaagca tttttttagc aaagcctctg ttgttgtagg cttctgcatc
    40201 ttcaggtttc agctctaatg ctttattaaa atctactatg gctttatcat acttttcaat
    40261 tttattatat gcactgcctc ttgaaacata agcccactca tgattgggtt cataaaaaat
    40321 tacatcagta aagttgccaa tagcttcagt atattttttt aagatagtgt tacaaaatcc
    40381 ttgttgtaaa attgcattta cgttggctac atccatgttt gccacatagg tgtagtctaa
    40441 caatgcttcc tgaaatttac cattattaaa ttttattttc ccctgattca tcagtgagtc
    40501 aatgcgcatt ttaaaatcag cagagtcaac ctgtgcattt gcctgtacac aaaaggcaga
    40561 taacaataca atgaacaata tttttacatt attcaaatgg ttctgaattt tcataatttt
    40621 tatcattaat caaatgtatt aaatgttgaa atgcttttac caagccatcg ttggtttttt
    40681 gtccataaga aaccggaaaa ctaatgcggc tgtagaaaag tctttttccc agaaactcac
    40741 gggtgacaca ttttttttgt gggttggtgc agttaatcag caacattcct gcttttgaat
    40801 tgtagtaaac cttagtggca tcaaggtctt taatagctgc ctgatcttct ctgtattttg
    40861 tttcgccatc aaaataagtg gctattaaaa cgccatagtt gatagaaact acatatttgc
    40921 ctgcaaattt ctgattgata tagtcgagcg tttgttgtac ggtgatgttt tcctgtgctc
    40981 tgagtgcggg tgaaaaaaca ctaagcatca gcagcaacag aaaaaacttt cttcttttgc
    41041 taaactcttt attgattcta tcgtaaacca ccaacattct tattttggaa attaggacta
    41101 tcgtttaaac tattaactaa ttttgtatcg gcattgaaac tttttaacgg aataaaatta
    41161 caaatgtttg gcattgggaa aagaattcat ttagttcctt taggggtttt tctcactatc
    41221 ttcatgttta tacatggtgt ttccactgcg cagacaaaga aaacattaac catcaatgcc
    41281 aagtttaaga ttgacggagg tggaagtttg ggtgatgcct atatggtttt gcagcgtgat
    41341 ggtcagtcta ttgaaactct tccagggcag tcgtcatata ctttaaatct tgaaataggg
    41401 gcagattata tcttgtcttt taacaagccc ggatacatca ccaaaaaaat aaatattaac
    41461 acaaccggtg ccgatgaaga aagagttgag cagggttttg agtcctatcc ttttacagta
    41521 gtgttgatga agcagtatga gggcgtaaat attgtagtgt ttaatcagcc tgttgcaaaa
    41581 attacttatt cgaaaaagta tgatgagttt gattatgata ctgattatac caagtcaatt
    41641 caatcggcaa ttcaaaaggc agaagatgat ttgaaaaaag cagagaaaga gtttaaaaag
    41701 aatccgccca aacctgccaa agacagcaca attgttgcca acaaacctaa ggcagaagaa
    41761 gtaaaacctc agaaagaaaa aaccaagact gcaccacagg aagaaccggt tgctgtaaaa
    41821 gaaaaaggca agaatgatga tacgaagaat gagtcgaaaa attctaataa aagcgctgcc
    41881 gctacgggtg gacaagacca accactaaag cctacacaag gcggaggaag tgatgagccg
    41941 gaaggtaata tagacattga aaagggcgca gatcaaaagg cagacattga acccacagca
    42001 gggaatgatg caattaaaaa taaaattggc ttgacaaaag gtgctgacca accacctgca
    42061 gcagaaccag ctaaaccaca accggtagtt attaaaactc cagaaactgc tgcacctgaa
    42121 aaaccaaaag aagtagtagt tcatacagca cctgagcctc ctgaaaatgt ttcagtaaga
    42181 cgtgaacaat tggccgaaga cagaaaaact gtttacaatg tttgggttac taagggcaat
    42241 aaaacggtgc tttatagtaa ggtcgtttat gcctggggtg gcatttattt ttttcgcgac
    42301 ctgaatactt ctatttctga aaacatgtat aagcaggccg atgcaggaat agtaaatttc
    42361 taggtgtctt cttcaactga aaaattgcag tggtttacct ttcgttttca ggtattcacc
    42421 caacttaaca tatttctgta ttcagcggct tttaaattgc atggagaaaa taaacactgc
    42481 aataaaatgt tgaatacata tttctgcatg aatctgttca aaccgcaaaa tgacagtcag
    42541 gtttcgttac ttgaagatat taaaacactg cgttctctaa aagggaatac atcaaggttt
    42601 gctattcttc gctctgtagc caaaacaaaa aagcatacac gcactgttta tcccgaaggc
    42661 tggaagtttg aataagtttt taatctttct tttagctaag cgaatttttt aatgcctcaa
    42721 caacattata cactattggg caaattttat aattgctcat attactttta ttccgataga
    42781 taaaaaaagg acggttcagg tgtgggtatt ctgaacatga tgaaggcctc agggtgtaaa
    42841 cggagcacaa atttttattg agtaaaaaac aaggattttt tctgagaatg taatgatgat
    42901 ttatttcatc atgttcaagc aattcgttaa gattttcgtg ttcactaatg cctgaatgat
    42961 tcagaatagg tcttatttct tcctctgtaa aaacaggact ttgttttttg cagcagttag
    43021 cacaggccgt acaatctatc tttggtgcaa tttcagcata caataggtgt acagccttat
    43081 caataactgc tgatttgtat ttctttttta ttgacttgaa ataatcaagc gtttcttgtt
    43141 cacgcagcga tgccaaccgt tcaacttctt cgggtaggca gacaagattc ataagagaaa
    43201 atttatttca caacagcagt aactgcaaac aatgctttca gtttatccat acagaaatca
    43261 acttcagcct ttgtgttgta gcgcgaaaat gaaaatcgta cagcaggtct ttcagggtcg
    43321 gcattgattc cacgtaatac atgtgagcca atctcactgc ctgacgaaca ggcactgccg
    43381 ccagatgctg caattcctga tatatcaagg ttaaaaagca acatgtcctt accttcgtat
    43441 ggcggaaaag aaacatttaa tactgtaaac aaacttcttg cactaacatc accattaaaa
    43501 gctactccag gaatttcttc ctgcaaacgt tgtttcatgt attcttttaa tccctgaata
    43561 tgtttacggt cttcttctaa ttctgcataa gcaagttcaa gagcttttgc taatcctata
    43621 ataccatagg cattttctgt tccgccacgc atattgcgtt cttgtgcacc acctgtaatc
    43681 agtggatgta tttttaattt atggttgatg taaataaatc ctacaccttt aggcccgtga
    43741 aacttatggg ctgcacaagt gaaaaagtca atattttttt gagacatgtc ataaggaaaa
    43801 tgtcccatag tttgaactgt atcacaatga aaaattgcat tgtattgttt gcagagttcg
    43861 ccaatgcggt caatgtcgtt aacggtgccc aactcattgt ttgcatgcat gagcgataca
    43921 aaagaacgtg gattgttttt tagtaattct tcgagatgct catagtcggc ttcaccttta
    43981 tcgttaagct ttacccaact aagtttgata ccctcttttt ctaaatcttc cagcgtatgc
    44041 gtaactgcat ggtgctcgat gggtgacgaa atagcatggg taattttatg atcgtgtatg
    44101 gtgcaacgaa tggccatatt gtttgcctct gttccaccgg aagtaaaaaa tatttcagat
    44161 ggtgctactt ttaataatgt agcaattgtt ttacgtgctt tctcaattgc cgatcgcatt
    44221 tctctaccaa aagaatggat ggatgatgga ttgccataaa tcttttgcat aacaggcaac
    44281 atggcttcaa taacttcctg tcttagtgga gttgttgcag cattgtcaaa ataaactttc
    44341 attgtttgtg tgtttgatta agtgtgatgg gggtcagact tttgcagggt ggcttattaa
    44401 ctctttgata tctgaaataa ttttttcagc aagattgttg gcagtagtga tgctgtcact
    44461 ttctgcataa atgcggatga tgggttcagt atttgattta cgaaggtgaa cccactcttt
    44521 atcaaattct atttttactc cgtcaatagt atttatttta tgctttttat acttctcctg
    44581 aatagattct aatactccgt caacatttat ttcaggagta agttcaatct tgtttttaga
    44641 aataaaataa tcaggataag aggagcgaag catagaaaca gtgtttccgt atttagcaag
    44701 atgcgtcaga aaaagtgcaa tgcctgccag tgcatcacgg ccataatgaa tgtcggggta
    44761 gataactcca ccatttcctt caccgccaat aacagcattt ttggctttca tcatttccac
    44821 cacgttaact tcgccaacgg cagatgcata gtggcttcca ccggcttttt cagtcacatc
    44881 gcgcagcgca cgtgttgacg aaagattact tactgtatta ccttttttgt ttttcagaac
    44941 gtagtctgct actgccacca aggtatattc ttcgccaaac atttcaccat cttcgcaaac
    45001 caatgcaaga cgatctacat cagggtcaac agaaattcct aaatgtgctt ttgatttttt
    45061 tactgtcttg ctcagttctg taaggtgttc gggtaatggc tcaggattgt gtggaaactg
    45121 tccatccggt gtgcagtaaa gttctatcac ttcttcaaca cctaatgctt ttaataatgg
    45181 tggcaatgca atgccgccag atgaattaac gcaatcaata acaattttga attttttaga
    45241 tgctatggct tttacatcaa ctaatggcaa tgcaagaatt gctgcaatgt gtttgtcaat
    45301 ggcatgtgca tctgttgtca ctgttcccaa ctgatttaca ttggcaaact caaacgattc
    45361 ctgctctgca attttaagaa gtgtttcgcc atcggcagca gaaataaatt cgcctttgct
    45421 gtttaacaac ttcagtgcat tccattgttt tggattatga cttgcagtta gaatgattcc
    45481 gccatgtgcc tgatgaaatg taacagccat ttcaactgtt ggtgtggttg aaaggcccac
    45541 atctatcaca tcaattccca tggcagcgag tgtgccacaa actaagttat gtatcattgg
    45601 tccgctgatg cgtgcatcac gacctaaaac aactttgcat tttgtttttg cagggaactg
    45661 tgctgcaagc catgtggcgt aagcagcaga aaattttact acatcaacag gggtgagtga
    45721 ctctccggat ttaccgccaa tagttcccct gatgcctgaa atagatttaa ttaatgtcac
    45781 aatctttgct ttttttacag tgcaaatata tcaaatcaaa gggtaatttt atgaatttaa
    45841 tagttggttt tcctgaatat cagtatttta ttctttctga caacagaaac ttttttcatg
    45901 ataactgctc tattttctta accaaaactt ctgcaatttt tctgttcgat tcatgtgtcc
    45961 atcctgcaat atgtggtgtg agaatagttt tatcggattt tatcatatag ccaaaaggtt
    46021 caggcagtgt ttctaacttt tcaaatgatg aagtttcgta ttcatgcaca tcaagacatg
    46081 caccttttac cttacctgtt ttgagatgtt taacaaggtc ggcagtgttc aggcatttac
    46141 ctcgtgctgt gttgatgatg taaatttctt ttttacactg actaataaag gtatcgttaa
    46201 tcatgtattt agtttctgct gtcaatggta cgtgcagact tataatgtct gattcctgtt
    46261 gcagttgttc aacagaaact tgttttataa aaggatattc agcagtgtca atttttttgt
    46321 atggatcgca agcaacaatg ttcacctcaa agcctgctaa tttttttgca aaggcactac
    46381 ccatattacc aaaaccaatg atgcctacag ttttgccctg taattctgtt ccacggtttt
    46441 cttctctcag ccagatgcca ttacgtactt ctttatcaga cttcattagt ttattcagaa
    46501 gagcaagtaa catcattaat gcatgttcgc ccacagcatc acgattagct tcgggtgcat
    46561 tcagacattt aatgttcttt gaaacagcat aagcaacatc aatattttcc atgcctgcac
    46621 caccgcgggc aataaacttt aaattagtgg catgttgcag caggtctttg tttaaaggta
    46681 tgcgacttct gattgctata ccctcatagt tgcctatgga ttgttgtatc ttctcaagac
    46741 tccattcata tccttctgta caatgaaatc ccatagctgt cagcttggga ataaatgttt
    46801 catgaaaggt gtctattaat aaaatttctt ttgacattat ttttaaaatt gacatgaaat
    46861 aaaaatcctc cgaatgtgca ggagaatgtt gtctgattaa ttctttatgt ttgtgtcttt
    46921 attctcaaga ttacaaattt gatggtattt gtcattgttg ccaattgcta attaggcatt
    46981 tcttgattcg taatctccct tctcatacag aataaaagtt aatagcacaa aggtaatttc
    47041 tataaatttt gtatccaaat ggctgaaata acagcaaata ttcactaagg aagtaagaaa
    47101 atagttgaca atgctaaaat ggtttattaa attgccgcaa aaagaagatt gtccattatc
    47161 ggttaaccat taaaaacaga gaaccgggtt tgctgattgg tatcttagtt attcaaagag
    47221 aaatgttttt ttgtttgaat gatgtaaggt ttacggaata atcttcaggt tttgggtaac
    47281 tcttatgcag gtgtataagt ataaccaaca ccaacaaaga aataaaccgt tatgcatgca
    47341 ttagacatat tacttttagg aggagctgta gtctgttttg ttctggcata tattattgct
    47401 tcacgcccgg cacgcgagtt gcgccaaagc aataaatcta agtcaaaatg gaatcatgtc
    47461 aatatggatt cagcttcatc agttcattcg cccgaaatga tgaaatatgt tcatgacaaa
    47521 acaggttaac tgaccttact ccggtttttt tgctcttaca actgatgtaa atgacgatag
    47581 aatattttat tttcttctga gttcaacagt gggtcatcat tcaaaatatt gatgactgca
    47641 tttcttgcat gttctaaaat ggctccatct tttgccaact cttcaatttt caggttcagt
    47701 agtccacgct gcccacgaag tacaacttcg caggtacggg agttaaattt taattgagaa
    47761 cctaaaataa atagtactta cttttccttt tgtgcttttt tccaaagttt aaattgttgt
    47821 tcatcgcacc agaaccattt tttattctta atccacgaaa ggtttgggtt cggtaaagaa
    47881 tcagacggaa taaaagttgc attcgatata ttttttgctc ttgtattttt aaattcaaat
    47941 acatcggcag cagttatagg ttcttgccat ttgaagcata tttttctaat tgtattttta
    48001 tcaattatct taaaccatac ataaccccaa ggtctgtttg gcctgctgtc tggtcctgtc
    48061 ataaaaaaac cttttagtgt gtcattgaaa atggaataac ccccccatag tgcaacacca
    48121 cccttgtagc ctattggatt gtttccttta aattgttcac catagaaaga atataaaaat
    48181 actccatctt cataaaatac cacatttctg gcaatagtat ccactacttt ttttacatta
    48241 ggtggatagc ccacattagt ttcaatatgc tcaagctgtg aatagtagcc ctttagttta
    48301 atcggaattt tcatgctagt gtctttataa caagaaaagg catctttaaa tacgcggctt
    48361 gtgggttgac aacccaatag caaccctaat aatgttaaga tagtaataaa atttcttatt
    48421 ttccccataa agagtatgga ttattatatc cgccataaaa atacatgcca gatttaaatt
    48481 ggctataatc tttataatac ggagccttcc caaagggtaa ataaaaaaaa ccatttttat
    48541 gcacccaatt ttgagttcta tcttgtacca aagaactact atactcccgt acttcgtagg
    48601 ttttagtgta acgaagtttt acttctgaaa gcatactgtt tcctttattt tttattgtgt
    48661 aaatataatt tattgttcac tttgtaaagc acagctttat gtcacactgc ccaagaagag
    48721 caacttcgcg ggtacggggt tttttatttt ctgctctgat gcaagccttt tttataccaa
    48781 tgttttttta agaaccaagc ctgtgtgcta tcgggtttga aatcggttga gtaaaaattg
    48841 tattgcacag gtggtgtata actattcaaa ttatttttaa gatctgattt acattgataa
    48901 caatgatacc acttaccaca aattctgttg gttattataa ttgtcgaatc gttttttaaa
    48961 tagccgctta attcaattaa ataccgatta tgaattatac tttcctgatt cgtttttgaa
    49021 aaatattcta ttttaattgt attatttttt atactgtaag ttccccacca ctctttattg
    49081 aaaggagaaa atttgaaagt gctttttatt gtattcacaa gaattgaatc aggattttgc
    49141 caaaatacag agtctatctt gtaaataaat ggtttatcta aaggtcttgt caaccatggc
    49201 ttgtctttat caaatccaaa ctcattttta acccaaaagc gattattctg accagtattt
    49261 ctaatatatg ccataccatt attaaaaagg aatatatatg cagttggaat acatctatct
    49321 attggttcat ttgccaaata gtagattcct tttattttta attttatact aagtgagtca
    49381 gtcttattct cgaaaaataa gttaaacctt ttatgtgaag taaagcatcc cataatgaaa
    49441 acgggtatca atagaataaa aatttgcctt cttaaaatct cagttttcat caatacaaag
    49501 aataaaaagc attaaactta ttgtgcccat acatttttaa aggagtatca taatctgtaa
    49561 taatatacgg tgatccatgc catccaggaa ttaaattatt ccgtacccgc gaagttgcac
    49621 ttcgtgggtt tttgtgcaaa gaagttttac ttctgaaatc ataccgtttc ctttattttt
    49681 attgcgtaaa tataatttat tgttcacttt gtaaagcaca gctttatgtc acactgccca
    49741 agaagtgcaa cttcgtgggt atggggaggg tacgagggag atgttttgga acatgaataa
    49801 tgttatcaat tatcttgaac cttttttatt ctatcaatag cgtacccgat tttaatgcca
    49861 attgccatat caggaagaac ataatttcct tttttatccc tgacgttttc tgctaaaaca
    49921 tcaacactat aaaaattatc ttctggtcgc aatcgttgat aatgtctaac atttttaact
    49981 cttaaaccta tacctgcatt gatatttaaa atgatttttg gaaataacct ccattctact
    50041 tcagacgtga tatttgcccc ataaactgat tttctgataa agtatttttc ttcatattct
    50101 tcagaagtaa ttttattttc aaacagattt ttagaagcat aatttatttt attgtaaaac
    50161 aacttagtgc ctaaataaaa tagcctttta cctttcatac taaagcatct ttttacctca
    50221 acagatgatt taaacccagt gccaccatat ccattaaaat aatctcttcc gaatattggc
    50281 gcaaaactaa tttcaaaacc atatctagta cccaaccagt attctgttcc aatcatagct
    50341 gattgatgtg atatttgatc aacaaaatta ataggggcta ttttgagaac tattttttta
    50401 ttggctattg agtcctctgc tttagcaatt gttatatgaa caattatcaa acagcaaaac
    50461 aaatacttta taaatttcat ttttttcaat ttattattct attagttggt tatttaataa
    50521 atgttagtgc gaataaatat tcccaccatc gtgcatttgt tattttttta tttgacggaa
    50581 ataatgaata tcggtctttt ctcccagaat atcttgtact tcgcaggttt ttgtgcaaag
    50641 aagttttact tctgaaacca taccgtttcc tttattttta ttgtgtaaaa ataatttatt
    50701 gttcactttg tacagcacag ctttatgaca cactggccac gaagtacaaa ttcgcggtta
    50761 cggggatatt taaataaaat gagtggcttc atagattatc aattcggtaa gtgccaggct
    50821 aactcacctt actccagtta ggttgctctc gcaactgatg caaatgacga taaaatattt
    50881 tattttcttc tgagttcaac agtgggtcat cattcaaaat attgatgact gcatttcttg
    50941 catgttctaa gatggcacca tcttttgcca actctgcaat tttcaggttc agtagtccgc
    51001 tttgctgtgt tcctgtaata tcgccaggac cacgtaactt taaatcggtt cctgcaattt
    51061 caaatccatc attggtttgc accattgttt tcaatctttc gcgcgcatcg gcagagagtt
    51121 tatttcccga cattaatata caaaaagact gctctgcacc tctgcctaca cgaccacgca
    51181 actgatgcaa ctgcgacaat ccaaaacgtt ctgcattttc aataatcata acagtggcat
    51241 taggtacatt aacaccaact tctataaccg ttgtagcaat catgatatgt gttttgccat
    51301 ttacaaatcg ctccatctcc tgttctttat cttttgcctt catctttcca tgtaccatgc
    51361 tcacaaaata ctcaggcaat ggaaaatctc tttgaatact gtcgtagccg gccatcaggt
    51421 tttgcaagtc taatttttct gattcttcaa tcaatggaaa aacaatataa atctgtcggc
    51481 caagttttat ttgctctttc ataaatccaa taacccgaag tcgtgatgat tcaaaaagat
    51541 gcatggtttt tattggcttt cttcctgcag gaagttcatc aattattgaa acatcaaggt
    51601 cgccataaag tgtcatggct aaagtgcgcg gaatgggtgt ggctgtcatc accagcacat
    51661 gtggaggttg ttgccgcatc cagagtttgg cacgttgctc cacaccaaaa cgatgttgct
    51721 catcaatgat tactatgcca aggtcgttga attttactct gtcttctatc aatgcatgtg
    51781 taccaatgag aatgtgcgtt tcgttagatg aaattctttc tataacagct ttctttttcg
    51841 attccggtaa tgagcctgta agcaattcaa cattcacggg taaactacta agcaatgatg
    51901 ttatggtttg aaaatgttgt gcagctaaaa tttctgttgg tgccatcagg caggcctgct
    51961 gtttgttgtc taatgccaat agcattgcaa aaagcgcaat aacggttttg ccactgccaa
    52021 catcaccttg caacaggcgg ttcatttgtt ttccgctgat ggtatctttt ctgatttctt
    52081 tcagtacccg tttttgtgcc tgtgtcagtt caaaaggaag atgttcatta aagaaggtgt
    52141 tgaagaggta gccaacttct gtaaacattc ggccgttttg agtagcagtt ctgttttgct
    52201 tgttcagcaa taatttaagt tgaatataaa acaactcttc aaatttcaaa cggaagcggg
    52261 ctttacgaag taagtcctca ttttcaggaa agtgaatctg aatcaatgca tcatgccgtg
    52321 gcatcagatt aaaagtcttt atcagttgtg gtgataaagt ttcttcaata gtaggaggaa
    52381 ggtctttaag tgccatgcgc ataattcgca tgatgccttt gctgtctata ccttttactt
    52441 tcgatttttc tgtggagtaa taaaacggtt gaaacccttt gaaaggttgt gcagtaaatt
    52501 tttcaagctc ttctatttca ggatggataa tactccactt gctgttaaat aaagcaggct
    52561 tgccaaaaac aacataactc gttccggctt ttatggaatc acgaatccat gttattccct
    52621 gaaaccaaac caaatccatg ctaccgcttt catctttaaa aacagcttgc aggcgtgtag
    52681 ttcgtttgtt gccaaaagct gttagtgaag taatcacacc tttaatctgc acataaggca
    52741 ggtcgtcatt cagttgtgca atggtgtaaa attttgaacg gtcaacatag cgaaacggaa
    52801 agtgatacaa aaggtcagaa acattatgaa tctctaactc cttacgaagc acagctgcac
    52861 gttgcggacc aactgtcttt acaaactcta tgtctttctg aaaataactc actcctgcct
    52921 ctgaaaaaat taaattaatt acatggctga aaaatacggg taaatgtgca aaaaataaaa
    52981 tccccggctg aattacaacc ggggaataac attaaaaaat tacaagtaaa tctatgtgat
    53041 tattactgtt gctttttaac atttacggca tttaagcctt ttttgccttg aactacttca
    53101 taggtaacaa ggtcattgtc cgcgatgcgg tcaattaatc ctgtctgatg aacaaatagg
    53161 tcacgaccac cttcatcttc aacgataaat ccaaatcctt tggagttgtt gtagaatttt
    53221 acttttccgg ttttcattaa ataaagaatt aaatttgtga attaatgctg caaatgtaaa
    53281 ctaatttgaa tatcatttat ttaaaataca aaataattta ttgtcggaac ctgacgtatg
    53341 accacaagcg gaaatattat tgacattttt aaccgaagga ttttccccgg aactgttcac
    53401 tttagcaagg gaataattac agaaattaca gaggacaatg ctgtttatga ccaatattta
    53461 ctacccggtt ttaccgatgc ccatatacat attgaaagca gcatgcttgt gccttatgaa
    53521 tttgctaaag tagccctgct tcatggcacc gtagcaactg ttagcgaccc gcatgaaatt
    53581 gccaatgttt gtggcctcaa aggtgtggaa tatatgattg agaatgcaaa agatgccaag
    53641 ttgaaattta attttggtgc accatcatgc gtgcctgcca ccacttttga aacagcaggt
    53701 gcagtgctta atgccgaaga tgtggcacaa cttcttgcac gacccgatat tcgctatctg
    53761 agcgaaatga tgaactaccc cggtgtactt tttggcgata tagaggtgat gaaaaaaatt
    53821 gagtcggctc acagactgaa taaagtgatt gatggtcatg cacccggttt aagaggtgat
    53881 gatgcccgaa agtatattgc tgcaggcata tctaccgacc atgaatgttt tacgcttgaa
    53941 gaagcattgg ataaggtaaa tggaggaatg aaaattctga tacgtgaagg ctctgctgca
    54001 cggaattttg aagccctgca tacgctcatt acttcacata cagcaatgac tatgttgtgt
    54061 agcgatgata aacatcctga tgaattgctt catggacacc tgaatcagtt agttgtccgc
    54121 gctctcaata aaggacacaa tctttttgat gttttgcaat gtgcttgtgt taatcctgtg
    54181 ttgcattata gtttatccgt aggattactt cgtgttggtg atccggcaga ttttatcgtt
    54241 atcaataacc cggaagtgtt tttagtctcg caaacatgga taaatggaga atgtgttgcc
    54301 aatgatggcc aaacaacatt gacaaaccgt agtcatgctc caataaataa ttttaatgca
    54361 tgggatgttt cggttcagga tattgaagtg aaaaaaaatt ccgaatcggt taaagttata
    54421 gaagcattgg atgggcagtt aattaccaac tgcattcagg caaagccatt agaaaaagac
    54481 ggactgtggg ttagcaatcc ggaaaacgat attcttaaaa ttgcagtcgt taaccgttat
    54541 agcaaacaaa agcctgttgt aggatttatt aaaaattttg gattaaagtt aggagctatt
    54601 gcatcaaccg ttgcacatga ctcgcacaat ataattgtca ccggtgttag tgatgctgat
    54661 attgttgatg ctgtaaattt acttatgcaa tcgggtggag gactgagtgc tgtatcagga
    54721 acagacaaaa aagttatagc cttgcctgta gcaggactga tgagtgataa aagttgcgaa
    54781 gaaattggtc gcgattatgc aagtattgat agtatggcaa aacaaatggg atctgcacta
    54841 agagcacctt ttatgacact atcatttatg gctttattgg taattccaca ttttaaaatt
    54901 ggcgataaag gattattcaa tgctgtaaag tttgaactaa cttcactgga ataatttatt
    54961 tgtgtttagc aatgaagtac tacttttaca aaaaaaatgt atgtggcaag aagtaaataa
    55021 ttcattggaa cgaacattta tttttaatga ttttaaacaa gcatttgcat ttatgacaca
    55081 tgtagctttt attgctgaga aaatgaatca tcatcccgac tggagcaatg tttataataa
    55141 agtcactatt aagttaaaca cacacgatgc aggaagtgtg gttacagaaa aagatcataa
    55201 acttgcagca gcaatagatg ttgtgttcaa tcaaataaat aaaaaagtat gagccgtttt
    55261 aaaaagtttt ttatatgggt agttgttctt gccataatcg gaacagcatt gtattttctt
    55321 gcaggaagtt atacctattc cgaaggaaca caaacaggct acttagtaaa attttcacgt
    55381 cagggttatg tttttaaaac ttatgaaggt gaactaaatc ttggtggtat gaatgccgaa
    55441 agcaattctg ttgtaaataa cctctggcat ttttctgttg acgaagatga taaggctgct
    55501 attgacagtc ttcgtgtcag cgaagggcgt gtgattaaag tttattaccg tgagattgta
    55561 cgcaattatg catggcaggg cgagacacct ttttttgttt acaaagtaga aaaagtaaaa
    55621 taggttttta tcctatacga tgcctgataa tagaatctat tttagccatt agttgcgaag
    55681 gcctgtaggt gcgccagtta agattatcaa gttcaccgcc aaacaataaa tagtaatcaa
    55741 tattggcacg ttgcatttca gacaaaacat gatctctgaa attgacagca gcaatccatc
    55801 ctgtttcatt cacttcttca aaccattcct gatagtaaca atcgtctgaa ttgtgaaagt
    55861 tgagtacgtt ttcaccaata agaataaaat ggcttatacc atgctccatc agattttcta
    55921 taacatctct ttttaaggtc atgatgtcat tattgatggc atcgttccat tcgcccatca
    55981 attcaataat ggcaaatgac ttttcatagt cgcaataaat aatttttata taaagcgttg
    56041 gcgagccaaa gtcatcccac tgcggatgga taaaatagtt gtaaatggta ttgctgtatt
    56101 caaactcact gtaaaccctt ccataaaaag gactcaactc atcttctgca gatgagtata
    56161 gatctaccca gttgtaaaaa ggctgtatat cctgcattgt ttgataataa aatgcaaaat
    56221 tacaacagac aaattcaaac tacaacagtt tatgatttat gattttttat tgctgcccgc
    56281 acgctgccgt attttttcag catcagagta gctttttttt cgggtacgcc aagttcatcc
    56341 attatcattc tgatgccacg gctgactaat ttattattgg taagctgcat gtcaaccatt
    56401 ttattgcctt taacatgacc aagttttatc atcactgcc
//
LOCUS       DAOOOI010000008        56149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_79_length_56149_cov_24.316950, whole genome shotgun sequence.
ACCESSION   DAOOOI010000008 DAOOOI010000000
VERSION     DAOOOI010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 56149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56149
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79_length_56149_cov_24.316950"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1523
                     /locus_tag="PKX59_02650"
     CDS             60..1523
                     /locus_tag="PKX59_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89796.1"
                     /translation="MDILRKTIKKLSDEEYQALLLQVSGKKKNKPYYVLEATRNNNHF
                     DDTEMMKQLQVNPSAYYTLKSRLNTRIAEILSKKVENPIRELMEEVVRVPATLYGTNR
                     DFSIRALKEIEKQLKEYDMSNELITVYKTLALLHIHHEDYEHYNKLYNKHVAYALAIS
                     KAEQMFFLFFKKLGTYQLTLDPDTLEEVIMLKRKIANIAEMYDSHRLFVLYNIVRIYY
                     LCSLPSKRQELKSLELEIESILKRINEIFNAFPIDTLYSNISPLVDILHYEYYQKIQN
                     QVRADHYLSNGINDTIIDWSEKTAMHFFIIQYLNSRVEKFLADGEMYRLNEFNQTIEK
                     TFDLNINEPYHYISFKRYIALCKFYSKDYQGAAKTIYDLRNNISLKQFTHTDVDSKLF
                     QGLCYCLMGEDSLAQQMISSIKRQIADFEHEFESTTIFIKILKTALKPSDFRRKIVRI
                     NELLIKFEEANNGDKPILKYIKIDDALIRKMTNPIKD"
     gene            1608..1961
                     /locus_tag="PKX59_02655"
     CDS             1608..1961
                     /locus_tag="PKX59_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009088046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOZ89797.1"
                     /translation="MDIEKFRNYCLSKQETTESFPFDEDTLVFKVAGKMFALVSLSEK
                     PMTVNLKMDPEIISEWRDRYSEITPGFHMNKQHWNSVIFNGSIPLAELKWLIDHSYSE
                     VVKKLPKKVRERFTI"
     gene            complement(1975..2298)
                     /locus_tag="PKX59_02660"
     CDS             complement(1975..2298)
                     /locus_tag="PKX59_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOZ89798.1"
                     /translation="MRFQKHIFVCTNQRKEGERKCCGEVHGLALVAAFKKKIKELQLP
                     VPIRAQRSGCLDACDYGPSVVVYPEGIFYGNVQLQDVDEIVEQHIKNNKPVERLIINF
                     DDKKV"
     gene            2390..>2768
                     /gene="frr"
                     /locus_tag="PKX59_02665"
     CDS             2390..>2768
                     /gene="frr"
                     /locus_tag="PKX59_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOZ89799.1"
                     /translation="MNDIAKKIIDTMQSHMDKSISHLETELAKIRAGKANPQMLESIH
                     VDYYGSQTPLHQVANVNTPDARTIVIQPWEKGMLGPIEKAIQIANLGFNPQNDGSIIR
                     IAVPPLTEERRKDLVKRCKAEGEN"
     assembly_gap    2769..2806
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2807..2964
                     /locus_tag="PKX59_02670"
     CDS             <2807..2964
                     /locus_tag="PKX59_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014599669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOZ89800.1"
                     /translation="EANDAIKKELKKGITEDEAREAETKIQQTTDGFISRVDKHLETK
                     EKEVMTV"
     gene            complement(2989..4836)
                     /locus_tag="PKX59_02675"
     CDS             complement(2989..4836)
                     /locus_tag="PKX59_02675"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOZ89801.1"
                     /translation="MTEVNNDFHADENQQAGEIQSENASITQPLQEEHLATLPEEHPE
                     TLPEQEENYNHLTKEELVKIAEGFAEGDVAAVKSKINALKNAFEQIQSSEKEQALSTF
                     LEEGGVKEDFKYNYTQSDDRFQAALKKYNKRKAEYAEQQEKLRENNFSVKQEILVAMK
                     NLIQNEENMQRAFDQFHELQAKWRATGPVPSGQLNDLWMTYKLYTDKFYENLKINREL
                     QELDFKKNLEAKIELCEKAEDLINEPSLNSALQTLHNLQNKWRETGHVGREKSNEIWE
                     RFKAASDKVYERRKEYFEQLKQRYAENLTAKNLLITKTEELLKDEPLKASEWMEKSKM
                     LVEVQNEWKKVGFADKKSNDEVWAKFRGLCDGFFVRKNDFFTSLKKEHAANLQLKNEL
                     CIQAEALIENTDWKKTTEELKRLQAEWKKTGFAGDRNNQKIWERFRKACDSFFERKNA
                     HYTSLESDYKENFDKKNALIEKAEQFTLGENIDESVEQLKDFQRQWSEIGMVPLEHKD
                     TIYAKFKAAIDKHFQTIRQHNKERFTSGHKPSFRSGKPGNEKNDLKHKINQLTGEINT
                     WENNLGFFARSKNADAVRKEFEDKINHAKEEIKRIKKQLDEISQTPKEQ"
     gene            5058..6584
                     /gene="rpoN"
                     /locus_tag="PKX59_02680"
     CDS             5058..6584
                     /gene="rpoN"
                     /locus_tag="PKX59_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOZ89802.1"
                     /translation="MLKQNLSQKLLQKLSPQQIQLMKLLQLPTIALEQRIKEEMEVNP
                     ALEEGAPEDVDEDTQQDEDEVPELEEGVEANEGEENKKDEFDNTSDDDIKERTDDDFD
                     VDDYITDEDIPYYKTSANNTSPDDERTEVPYGSSVTFQETLLAQLGMVALNEKQMQMA
                     KHLIGSLDEDGYLRRDLNSIVNDLAFTQNIDTNADELLQVLQVIQTFDPPGVGARNLQ
                     ECLLLQLQRKSNSKSVNRAIEIIKNYMDEFSKKHFEKIARLMNIPDEYLKEAMNEILK
                     LNPRPGGDISAETKSVQHIVPDFIITNNVGQLELTLNSRNAPDLRINREYNEMMEGYA
                     KSKKQDRSQKEAILYLKQKIDSAKWFIDAIRQRQATLLHTMQAIMNYQKEYFLEGDER
                     LLKPMILKDIADVVGLDISTVSRVANSKYVQTPFGTFLIKTFFSEGMATDSGEEVSTR
                     EVKKILSDAIEGEDKKKPLPDDRLAKILKDKGYNVARRTIAKYREQLNIPVARLRKEF
                     "
     gene            6587..7204
                     /locus_tag="PKX59_02685"
     CDS             6587..7204
                     /locus_tag="PKX59_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89803.1"
                     /translation="MLNRFFSVVTYLTHPLLIPTYLLLILFNSDTYLSYTLSPALQRI
                     ILSAVFVLTCLMPFLSSVLLLKKQGVQGLLMEDRQSRTIPYFFTLIYYVACYYFLLKL
                     PVSSVIKILILAAACCIALAFTINFFWKISIHLIGLGGMAGAFYVLSLLLSSEFILPF
                     VLSIILAGLTASARLMKNAHSPSQLYFGFIAGFVCEYIFIMYSLK"
     assembly_gap    7279..7316
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7413..9410)
                     /locus_tag="PKX59_02690"
     CDS             complement(7413..9410)
                     /locus_tag="PKX59_02690"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ89804.1"
                     /translation="MKWEPGTKKLVALVGRVYWRKGFFDMALKCHFDVLKYYESRNDE
                     PQIAELLTFIGQDYADGGRYEDALSYFKKAGAYYEKLRDSSKMGGNYLLFGFVYESQG
                     NYVENVAANYKALKIFEAIGDKYGEAIVLSNIAGVYSTQGNYSEALKLYLSAIKAFDK
                     AKDIINLTVAYTSVGQTYQQLGNTTEAISYYNKAYEIAKSISNDFAMATASESLGDVY
                     FQKQNYQKAIQFFQAAANSFRVVSDYFDLASALSKLSICQTILGNYDSAKKNLDEAWT
                     LAEKLDSKVPVTEYYHAVALLDSATSDWQGAYNNYKNYIILRDSTYNRENTKKILLQQ
                     IQYENNKKDALLKAEQEKNAVAQRVFRNSMIAGASILLLLLMLLFHRYRFKQKTNLQL
                     KEAYQHLQATQQQLVQQEKLASLGVLTAGIAHEIKNPLNFVTNFASLSNEILIELKEA
                     QTVQDKDEAMNLLHENLVKITEHGHRADNIVKRMLEHSRTNTEDRQKTNINSVCDEYL
                     DLAYHGKRAILQNFNCDLIKNFDPNLPQVICNGQDISRVVLNLIDNAFDELYECERLK
                     LKGENFKPQLVITTASIPQTSRQKSNRKIFISVRDNGRGISEKMKQKIFEPFFTTKPT
                     GKGTGLGLSLSFDIINTHKGTIEVTSKELEGTEFKIILPVS"
     gene            9897..10577
                     /locus_tag="PKX59_02695"
     CDS             9897..10577
                     /locus_tag="PKX59_02695"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HOZ89805.1"
                     /translation="MKKLFTTLVILSLAVVVQAAHPLQSALAITSVDGSLLTVSIDRA
                     PFSNSSTNIYTDGLRAGNHYIKVMRLAGHPRHPVYRTVYAGYVFIPEASEVIAEVNRN
                     RFRITGSRPFDYAPQVYEDPHYYPQHLRCEVDDYEFNQMIQSLGNYNFESTRMTVAKQ
                     LINNNYFNSRQVGRLMQQMTFESSKLELAKYAYSKTLDKNNYFLVNDQFSFESSIAEL
                     ADYIALRN"
     gene            complement(10626..11015)
                     /locus_tag="PKX59_02700"
     CDS             complement(10626..11015)
                     /locus_tag="PKX59_02700"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOZ89806.1"
                     /translation="MIKIVKSAFFIICCILIHLQIKAQTSDDSLTHVLINKHIAYNLA
                     KPYSQGYRIQLYFGSQRDKAYEFRTEFIKLYPQTAAYVLYQQPNFKLRVGDFRTRLDA
                     QKRLKELQTYYPSAFIVKDDIKIKIDE"
     gene            11297..12106
                     /locus_tag="PKX59_02705"
     CDS             11297..12106
                     /locus_tag="PKX59_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOZ89807.1"
                     /translation="MLLILGFSAGVKAQDGKALFDANCKSCHAMNDVVIGPALKDVHK
                     KFSAEWLHKWIKNSQALIKAGDKDAVAVFNEYNKMVMPNQNLSDAEIDAVLAYIGKES
                     EQAPVAAAPTAPAPGTVAPEKDNSTLYWVIAIILILLALAATLNKIRKGLEYAVREKE
                     GIPHPPHLEPKEARSLWISKNKKLIAVVLLLLVVLGSVKGWYALKDIGVYTNYEPEQP
                     IHFSHKLHAGDNKIACLYCHSGADKSRHANIPSASTCMNCHKYVKGSCVWR"
     gene            12093..12479
                     /locus_tag="PKX59_02710"
     CDS             12093..12479
                     /locus_tag="PKX59_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOZ89808.1"
                     /translation="MYGDKEIKKIYAALDWDGSKFGTNQKPIQWIRVHSLPALAYFNH
                     AQHVTVGKIECQTCHGPVQEMDVVKQFSPLTMGWCINCHRETEVKMDGNGYYDEVHKT
                     LAAKYGKDAKLTVDKIGGLDCARCHY"
     gene            12550..15651
                     /locus_tag="PKX59_02715"
     CDS             12550..15651
                     /locus_tag="PKX59_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HOZ89809.1"
                     /translation="MEKRYWKGVEELRNDAEFVRLKNNEFFEEIPVDEVLGKKAESKD
                     ATPRRDFLKFLGFGVAAASMAACEAPVRKTIPYLISPENLVPGIPNYYASTFFDGHDY
                     ASVVVKTREGRPIKVDGNELSPLTKGGANARVQASVLDLYDSERIKSPIAKGKAASWD
                     TVNKEIKEKLAAANTKQAKIRIVSSTIISPTTKKAIESFTTKYPTAKLVTYDAMSQYG
                     IIKAHQLAFGKAALPNYSFDKADVIVSFGADFLNNWIAPIEYARQYAQTRKLDDGKKT
                     MSKHIQFEAALSLTGSNADNRYKLKASQTGNALLSLYNAIASKAGAATVSAGKTDADA
                     AINSTANDLWNAKGKSLVVCGTNNANDQLVVAAINNILGNYGATISIAKHSNVRQGND
                     EAMAELLREMNAGEVGVVIFYNANPLYNYPDTTAFTSALNKAEVKISLSDRNDETAIK
                     CDYICPDSHYLESWNDAEPVRNMFSLVQPTIQKLFDTKQSQELFLLWSDAPVTDYHEF
                     LMQNWRQGFLATATGMASVPAWEKVLQEGVWNGEVTESEPAFVGNVNDAASAIGARKS
                     GGLDIVVYEKAGIGNGRHANNPWLQELPDPVSKICWDNYFAIAPSMAKEKGLVQGNII
                     EVKSGNVTLKGPVLLQPGQAKDTIAVAAGYGRTNSGKAGNKVGMNAYPLLTFSEGTFN
                     YTNSGASLNKTVEADYTLATTQSHHTMMGRAIVKETTLEEYIKDPKAGNEEEKFHSYK
                     GKVSAKELDLWATKDHPGFDRPINAWGMSIDLNSCIGCGACVVACTAENNVPVVGKEE
                     IQRSREMHWIRIDRYYTSDMDKEKAHEQGVGKLSMYGFMEEPAENPEIVFQPVMCQHC
                     NHAPCETVCPVAATTHSEDGLNMMAYNRCVGTRYCANNCPYKVRRFNWFKYSDNPQFD
                     YNMNNDYGKMVLNPDVIVRSRGVMEKCSMCVQRIQYGKLEAKKAGHPVKDGAINTACA
                     QTCPTNAITFGDYNDKSSRLVKQSGSERMYHLLEELNVQPSINYLTKVRNTTGKTNNS
                     HKA"
     gene            15689..17074
                     /gene="nrfD"
                     /locus_tag="PKX59_02720"
     CDS             15689..17074
                     /gene="nrfD"
                     /locus_tag="PKX59_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010177372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOZ89810.1"
                     /translation="MSNVESPLRPTLIEGDKTLHDITEDVCRPIESKPNKYWWMVFSV
                     ACAALLWWIICVAYTVGTGLGVWGLNKTVGWAWDITNFVWWVGIGHAGTLISAILLLF
                     RQKWRLSINRSAEAMTIFAVICAASFIVAHMGRPWVAYWPLPFANQFGSLWVNFNSPL
                     VWDVFAISTYFSVSLVFWYTGLIPDFAAVRDRAKTKARKFWYSALSMGWVGSAKNWQR
                     FEEVSLVLAGISTPLVLSVHSVVSMDFATALVPGWHTTIFPPYFVAGAIFSGFAMVQT
                     LLIITRKVLNLEEYITIYHIDMMNRIILITGSIVGCAYLTEFFIAWYSGVEFEQYAFI
                     NRATGPYWWAYWSMMTCNVISPQLMWSSKLRRNLFVTFFLSIIVNIGMWFERFVIIVT
                     SLHRDFVPSSWTMFHPTWVEIGLFIGSIGLFFVPFLLFTRYFPVIAMSELKTILKSSG
                     SQSKKGVQHHD"
     gene            17067..17579
                     /locus_tag="PKX59_02725"
     CDS             17067..17579
                     /locus_tag="PKX59_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HOZ89811.1"
                     /translation="MTKQLHGIFNDDEVLLNAIKELKSKGIKCTNAYSPFPVHGLDSA
                     LGLKRTRISIASFLYGATGTCLALLMMWYMNIWDWPMDIGGKPSWNLYKNIPAFIPIT
                     FEFTVLCAAHGMVITFYLRSKLLPGVTPNVIHDRMHDDHFVLTVEGKVSDETTIRESL
                     KQVGAVEIRS"
     gene            17593..18198
                     /locus_tag="PKX59_02730"
     CDS             17593..18198
                     /locus_tag="PKX59_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOZ89812.1"
                     /translation="MKNTHIYFNVIIGGMILLSACGDKQSPGLEYMPDMYRTPAVVAY
                     SPSHITKDSVSALAPVAGTVARGQYVDFIYPNTNEGYEAAGLELKNPFQPTAENMERG
                     KTEFDTYCAHCHGEKGDGKGWLKIKGDPFPVPSYTSAAIKDLPDGKMFFSITYGKNLM
                     GSHAAQVSADDRWKIILYINHLQGDEPGTVVADSSATAQNK"
     gene            18227..19429
                     /locus_tag="PKX59_02735"
     CDS             18227..19429
                     /locus_tag="PKX59_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HOZ89813.1"
                     /translation="MQDNLTYTFSSKLKTLSFILMAIGAISIGISFATNPDQTWANLM
                     HNNFYFLTIALGGLFFYAVQYAAEVGWSVYIIRIPLAMSQYLWISGVIMLILFFAAGH
                     HIWHWTHHELVMETLPDGKPNPDYDEILAGKSAYLNVPFFLIRMVAYFVIWYGFAVLL
                     RKHAMKEDLSGAIEHHNRSRKLSIIFLVLFAVTSSTSAWDFVMSIDSHWFSTLYGWYT
                     FSGIFVSSLGVIALIIVYLRSQGYLHHLNENHLHDVGKFIFAFSIFWTYLWFAQFMLI
                     WYANLPEEVTYFMERQDYYKTLFVTNFLVNFILPFVVLMTRDAKRKLRLLAILGSVIF
                     IGHWIDTYLMVVPGIMHEKWTIGVLEIGTTLGFMGMFIFTVFTALTKLSLLPQKHPML
                     QESEHFTL"
     gene            19471..20496
                     /locus_tag="PKX59_02740"
     CDS             19471..20496
                     /locus_tag="PKX59_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HOZ89814.1"
                     /translation="MTLLITISILLGILVLVRLMTIVQLSSELTGSSDSQEEQIRHNN
                     SNGFGFIVFMVLGLGLFIYMTIDYSKHLLPVSASEHGVGLDKLLNINWAIIGVVFFIT
                     QIALFYFSYKYRDNKKHEAYFYPENYKLELIWTIVPTIVLTTLIITGLKQWNGIFSEK
                     ENSMNVQVYGYQFAWITRYSGPDNTLGKSNYKLITDDNPLGLDASDPAVKDDIYTTGN
                     EMHIPLGQELKFQFNARDVIHSAYFPHFRAQMNLVPGMNTFFSFKPTITTAEMRKITG
                     NDKFDYVLLCNKICGVAHYTMKMKVVVDTPADFKAWLKSQKKASEVKAAPPVSALNST
                     VKGSNSI"
     gene            20548..22368
                     /locus_tag="PKX59_02745"
     CDS             20548..22368
                     /locus_tag="PKX59_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOZ89815.1"
                     /translation="MEVKQTAGVAVEHHDDHTHEHDHHETFLTKYIFSQDHKTIAKQY
                     LITAIIMAFFGMYMSLIFRLQLGFPETKFAFLESLIGKWGKGGRLDPGFYMALVTIHG
                     TIMVFFVLTGGLSGTFSNLLIPLQVGARDMASGFLNMLSFWFFFLSCAVMTYSVYIET
                     GPAAGGWTVYPPLSALPQAMPGSGLGMTLWLVSMVFFIIQSLLGGINYICTVINLRTK
                     GMSMTRLPLTVWAFFITAILGVLTFPVLFAAALLLVFDRSFGTSFYLSDIFIGGVALP
                     QSGGSPVLYEHLFWFLGHPEVYIIILPAFGLTSEVLSTMGRKPIFGYKAMVGSLMAIA
                     FLSCIVWGHHMFVTGMNPFLGAVFTFTTLLVAIPSAVKVFNYLATLWRAKIQLTPAMC
                     FAIGLVSLFISGGLTGIILGDSALDINVHDTYFVVAHFHVVMGASAIFGMLAGVYHWF
                     PRMFGRMMNKTLGYVHFWITLIAVYGVFFPMHFIGLGGVPRRYYSNSAYEGFDVFLNI
                     NTLITVFAFLAAVAQVIFLFNFVYSIFKGKKAPQNPWHSNTLEWTSPAGPGIHGNWNG
                     PVPTVHRWPYDYSKPGADDDYIPQTVPYSQTKNSNLPGEE"
     gene            22507..22983
                     /locus_tag="PKX59_02750"
     CDS             22507..22983
                     /locus_tag="PKX59_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOZ89816.1"
                     /translation="MKSSFRLIRQCIFVFLIALIFNSDNLFAFSDSTKVVVKKPKKEK
                     YYKRSRIAVEDVRNYMDSIGILNSDIVLKQALLETGHFKAKLLMDKNNLFAFRFTKRY
                     MSFINWQASVDYYKKWQDKFYTNQSENYYDFLKRIRYARFPHYIKVLKQVKARTAQ"
     gene            22999..24396
                     /locus_tag="PKX59_02755"
     CDS             22999..24396
                     /locus_tag="PKX59_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOZ89817.1"
                     /translation="MKNSRNNEPIKILFFLTVILSVISIMPKYLTGSKKINLYSDIIH
                     KENKKEKPQSLAIVNDSVFFKNDTVKQVLTDTAKSTATKINYKSGELDLTRFFEALHN
                     HENENGKIRIAWFGDSMIEGDLVTQDFRKLMQKIYGGSGVGFVPVTSPVAQFRQTIRQ
                     TFSDDWEVYSFMKKPKQNYKLGIGGYSFVSSKGSWVDYKASRFYPVYSEVKMICRQCP
                     PKSFILNEDTSLVFPVDSIGSFKEVELIRNNPIKEVKLTFKKPNCEVHGLYFDKGNGV
                     YVDNFSFRGNSGIPLSNIPFGELKSLGAKENYKLIILSYGLNVVAHDVKNYSWYASSF
                     QKTLNYIKEAFPDASILVMSVGDKSYRAEDGYETEPDIPIFVELQKRIAQRSDVAFYN
                     LYEAMGGYNSMVSWAEGNPQLANLDYTHPNFAGGRKIAGMLYDDIIKSYDRYSKTVET
                     MEVKRDTMIKPQAKQ"
     gene            24393..25634
                     /locus_tag="PKX59_02760"
     CDS             24393..25634
                     /locus_tag="PKX59_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOZ89818.1"
                     /translation="MKRIILIFSLLIYCTKCHAQSDSDTTMPVLQYEKFVEYKMSLLK
                     FLKPDAIDIEQKEALKNFYSKLLQSRADGKKVSIVHIGDSHLQAGYFSGMVRRGLQQR
                     YGNAGRGIVFPYKLARSNGPHDYVSFSQNSWKGNRNVITQDSLSTGITGFSVTTNDSS
                     PSVGISLRNNEGIDYRFNRVQVFYKPDSTFMTASDSLAIIETVNPIKLATGINEFRFS
                     DYLSNCKINISSDTTYTLFGLNLLNDSSGVVYHNIGVNGAEYKSFLGQPMFFEQVKEL
                     NADLYILSFGTNEAFAKYFDGTVFIQTVDSFITKLRNVSPTASILITSPTESCKRIRR
                     GVYVPNPNVIAIKNLLQKYCMDNKIAFFDLYECSGGKGSMLRFAKNKMSDARRIHLNR
                     SGYEMQGLMLLQALLKPLSEQ"
     gene            25631..27100
                     /locus_tag="PKX59_02765"
     CDS             25631..27100
                     /locus_tag="PKX59_02765"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOZ89819.1"
                     /translation="MTIDWEKLGHQFLYDKANPLLFNSSFFLFYLLLFLFVFRLLQRT
                     GTLRIVAVTLFSLYFFYKASGVYVLLVLAAAVIDFNLSNFIYNATSDFRRKALLVFSV
                     VINLGLLAYFKYTNLLIDLYNAILTGNVQPIDVYLPIGISFYTFENLSYTIDVYRRQF
                     VPVKKFIDYLFFLSFFPKLMMGPIVRAADFIPQIRKEIALTAHDVSKGLFLILTGLFK
                     KVVISDFIYLNLVQYVFDDPLRHTGMECLFAVYGYALVIYCDFSGYSDMAIGIAKWIG
                     IDININFLAPYQSASITEFWRRWHISLSSWLRDYLYIPLGGNRKGEVRTYINLFITML
                     LGGLWHGASLNFIFWGALHGVALAFHKLWKAAYPSHFSGRLTRIAGIIITFHFVCLCW
                     IFFKCTTFTDSFNMIHQIVFNFYAEGWTLFSANYSEVLLVMLLGFVLHFLPIRWNEII
                     INYTEKWHWLLKASVATAVVIFVIVLKQANPVMPVYLQF"
     gene            27177..28958
                     /locus_tag="PKX59_02770"
     CDS             27177..28958
                     /locus_tag="PKX59_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HOZ89820.1"
                     /translation="MKRLFIVISVFFIAACKDSGKQSIKDTSVLLKEITDGYYQERMR
                     YFPLEATANADNRYNDLLPIDISDAYIDTLRQFYHRYSEKLLTINKPELTGQDLISYE
                     VLQYTLNIEEEGLKFPSNLMPVNQFWGMHLTFSQLGSGTSSQPFKTVKDYDNFLQRIS
                     AFVAYQDTSINNMKRGMLQGFVPARILIERTIPQYKSLIANKVEESVFYGPIKNMPDS
                     FSDADKKRLTDAYSNAILNQLNPSFEKMAVFLEKEYLPACLPVAGISNIPGGKEYYNW
                     LANKWTTTTMSPDEIYNLGLKEVERLHSEMDKVKAETGFKGSLKEFFHFIHTDPQFFP
                     FKKDSDVIAAYLAIEDRMKPQLKKLFNHVPKAAFEVRQTEAYRAASASAEYNTAAPDG
                     SRPGVFYVPILDATKYNVVGMEDLFLHEAIPGHHYQISLQQENTSLPEFRRFGWFGAY
                     GEGWALYTESLGKELGLYTDPYQYFGCLSEEMHRAIRLVVDVGIHLKGWTREKAIQYS
                     LDNEMESEADITAEIERYMAIPGQALSYKIGQLKIIELRQKAEEQLKDKFSIQKFHDQ
                     LLSYGCLPLSVLEKSIKLWIKEQKKQG"
     gene            29041..30027
                     /locus_tag="PKX59_02775"
     CDS             29041..30027
                     /locus_tag="PKX59_02775"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HOZ89821.1"
                     /translation="MKQLIENFPSQMREAMTIGETFKATSWPHEIKNVLVTGLGGSGI
                     GGTIVSQLCEKEMPIPFMVNKDYFMPAFVNKHSLVVVSSYSGNTEETLHAMDMAIKQG
                     AKVVCVSSGGKTVELAKQHGLDHILIPGGMPPRSCYGYSSTQLFYILHAFKLIGDGFK
                     ASLKNAVDLLDAESANIKNASHKLASELIGKIPVIYSASNNEGVVVRFRQQVQENGKM
                     LCWHHVFPEMTHNELVGWTQPHNDIAVVLFRDKDDFFRTAKRMDICKEIFGRYTPHIY
                     EVWSKGGSPIEKAIYLIHFGDWTSWYLSEIRNIDCTEVKVIDYLKSEMAKLN"
     gene            30052..30681
                     /gene="tmk"
                     /locus_tag="PKX59_02780"
     CDS             30052..30681
                     /gene="tmk"
                     /locus_tag="PKX59_02780"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOZ89822.1"
                     /translation="MKKENLFIAVEGIDGSGKSTQVQLLKANLEEAGYKVYATNEPTN
                     FEAGKLIRSIFNHRVNADQHTIAALFVADRLQHLLDKENGILAMLEKGYTVITDRYYF
                     SSYAYHSAYVEMDWVMSINAKCAALLKPDLNIFVDVDPETAFKRIIATRTSTELYETK
                     ENLRKVYDNYLKAFKLLKNTESIVFVNGKNDVQQVAADIFKTVKNLLHT"
     gene            30744..32378
                     /locus_tag="PKX59_02785"
     CDS             30744..32378
                     /locus_tag="PKX59_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOZ89823.1"
                     /translation="MNKKHPKALPYLFLSEMWERFGFYLMLGIFFLYMTDTQRGGMAM
                     DRKEASDIFGTFIALVYLTPFVGGLIADRVLGYRVSITLGGILMGIGYCGLAIPGMTA
                     FYVSLLLIIIGNGFFKPNISTILGNVYNDPEYKPLKDSGYNIFYMGINIGAFICNFFG
                     SYLRNNFSWGAAFAAAGIGMFVGVIIFWIGNKHYAHADVRKPVKPEDMPVSKILGITL
                     LPALIAGYIGWIIPENIFGSDSTDAFILGALPVAGYYVNILLRSDKEDRRPLAALLAV
                     FAVAIVFWAVFKQNGTALTTWAESYTNREVPQSVEPVAKVLGVTQTVTFSKDSVPLTD
                     SQFRVARDENKKAIKTWDYPLYYKNVPADKLPKEGESLTLISTELGQSINPFWVVILT
                     PVIVAFWAFLKRRNSEPSTTWKIFYGFLITSLSTLVMVAATYACHNGLEKSSMWWLIA
                     SYGVITVGELCLSPMALSLVSKLSPPRLTALMMGGWFLSTSIGNKLSGVLASMWDEYE
                     HKANFFLVNFTLVMITTIIILLMLKWLNKIVKEHGA"
     gene            32590..35109
                     /locus_tag="PKX59_02790"
     CDS             32590..35109
                     /locus_tag="PKX59_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89824.1"
                     /translation="MRLLSTAVILLGCLCAQAQISITNNEMPSAGDTARFTKAVVNPL
                     INFSATGTNHTWSFGNLRNNGQDLKSYQNVSSTGFIYSLFFSNLPFNPNRANVTEPGQ
                     ALPSNPLLTITDPYNFYYRSSSVYKQVGFGAEIANIPMPVAFSQHDIIYNLPLNYNNS
                     DTSHSAWNIGLPGLGYYGLTQTRINHVDGWGTLTTPHGTFNTLRVKTQLYIKDTISVD
                     TLNLNFALNRPKTTQYKWLANNEIIPVLQITTTEILGLEVVTEIIYRDDYNTVSPGSL
                     NAAYCAGSNVSIPYTATGSFNGSGFLQTANSFTAQLSDATGSFANAVNIGSVTSRVSG
                     NINAVIPANTPVGNGYRIRIISSSPALTGGDNGFDLRIENNAVASIVNQGLSAFCSGN
                     SALLQSVNVDSGYTYQWQLNGTDISGATTDNYLASATGNYTLNITNSCGTITSNAAAI
                     NVLPMPTVQVTASGPLTLCLGTTVTLSTLFDSTYSYQWQLNGVDISGATTENYTAASG
                     GDYTVVVSNSCGNVTSSVQTVSVQSAPVAGSLTAASSTICHGDSVQINYSLQSAGITW
                     LHNGNILSGITTNSHFANSAGVYSVVESDLCGSDTSVAVILTADSLPTAVITASGALS
                     FCAGGDVVLTAATGNNYAWQWYMNGTPLATTQLITATQSGNYEVEITNACGSVMSTLV
                     AVTAFPLPVVPTITLSNDSLLATTTVGTYQWFYNGNAITGATNDFYVPSQNGNYSLMV
                     TDANGCSSMSAVYNMTTVGLQTIFKQAHNVFPNPFNDYLTIELPQTENAIILTVEGKQ
                     LWSGTLHQGKNVLNMQQFVSGMYILQLNNENGITNYKLVKR"
     gene            complement(35155..35850)
                     /locus_tag="PKX59_02795"
     CDS             complement(35155..35850)
                     /locus_tag="PKX59_02795"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOZ89825.1"
                     /translation="MAILILYQSIQLKKYIALVSVILILDQCLKFWIKTNMYLGQEYH
                     IAGNWFIIHFTENNGMAFGMELFGKKFLSLFRIGVMFALAWYVWHLHKTKAHHIYTTA
                     MALIFAGAVGNIIDSIFYGRLFSSSEFGVATFMPDAGGYAGWLHGKVVDMFYFPIIQT
                     HYPQWFPLWGGEEFIFFRPVFNIADSAITAGVLLIILFNRTIFKHIETTPETEQTTVE
                     MRSEELNTANHNS"
     gene            complement(35889..36281)
                     /locus_tag="PKX59_02800"
     CDS             complement(35889..36281)
                     /locus_tag="PKX59_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOZ89826.1"
                     /translation="MENSPDKTRYSDAELNEFRELIIQKLTDAKVELNNLQAFLNNSN
                     EHGTDDTASSFKMLEDGSDTLAKEEAGQLAARQRKFIEQLENALVRIENKTYGLCRVT
                     GKLIPKERLRAVPHTTQSIEAKVQQYRD"
     gene            36412..36903
                     /locus_tag="PKX59_02805"
     CDS             36412..36903
                     /locus_tag="PKX59_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89827.1"
                     /translation="MLRLRELSLGFSTCLIIDVADLLLSSFCDFAVTPGFIILIGFGL
                     TTIAGLTTFALATGFAFATFFATALLLITLLFATAFLVTVFDLAGLPFATAFLATTFF
                     ATGLLLTAAFFTTLTTFLTAVFLFFGVAFFAAAFFGAAFFATGFFFALLAIFKSFLFQ
                     LMF"
     gene            complement(36872..40336)
                     /gene="ileS"
                     /locus_tag="PKX59_02810"
     CDS             complement(36872..40336)
                     /gene="ileS"
                     /locus_tag="PKX59_02810"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOZ89828.1"
                     /translation="MSHKPYPEFKQLDLAAIANDVLLKWDNEQTFDASITSRQGAKAY
                     NFYEGPPSANGLPGIHHVMARAIKDIFCRFKTLQGYQVKRKGGWDTHGLPIELSVEKT
                     LGITKEDIGKKISVEDYNKACRKEVMKYKEVWDDLTRKMGYWVDLKNPYITFENGYIE
                     TCWYLLKELYKKDLLYKGYTIQPYSPAAGTGLSSHELNQPGTYKIVKDTTVVAQFRIK
                     KETLPQSLQKDLQNINCDFYFLAWTTTPWTLPSNSALAVGEKIGYVWVKTFNQYTNEP
                     IVVVLAKDLFAKYFSEKNAALSLEDFKPGDKQIPFKVIAECKGSTLTGIAYEQLLPYV
                     KPDGKAFVAVAGDFVSTEDGTGIVHIAPTFGADDFRTAKQQGISAILVTDEGGNPSPL
                     VDKRGRFVKEVTDFAGEYVKEEYLTTNEKEAERQKQGRDKYLSVDERIAIKLKQENKA
                     FKVEKYEHNYPHCWRTDKPVLYYPLDSWFIRTTACKDKMIALNKTINWKPESTGIGRF
                     GNWLENLVDWNLSRSRYWGIPLPIWRSEDEKEEKCIGSIIELRTEIEKAVAAGFMDAH
                     HLEHVDASKSDFDLHRPYVDNIILISDTGKKMYRETDLIDVWFDSGAMPYAQWQLDQE
                     KLSKGLPMPFNEGFNTGYPADFIAEGVDQTRGWFFTLHAIAVMLFDSVAFKNVVSNGL
                     VLDKNGNKMSKRLGNAVDPFETIGKYGPDATRWYMISNAQPWDNLKFNVDGIAEVQRK
                     FFGTLYNTYSFFALYANIDGFVPDEKNKVPVQDRSELDRWILSKLNNLVQRVTDSLND
                     YEPTFAARQIEEFVDEHLSNWYVRLSRRRFWRSEMNTDKQSAYETLFECLMVTAQLMS
                     PVAPFFSDWLYRNLTDSMRSNAIAAKTPLQYTSVHLTYLTKAETKMIDTALEERMDFA
                     QRISSMVLALRKKVNIRVRQPLQKILIPVDSPKQQMAIEQVEKLILTEVNVKEMEFVT
                     DASGLLVKKIKANFKVLGKKLGALMKDAAAAIAQMTQDDIRNLESKGTFDLRINQNIV
                     AISVEDVEISSEDIPGWQVNSNGGLTVALDISISPQLRDEGIARELINRVQNLRKDKG
                     FEVTDKILVKLKNQPSIITAIESNLDYICAEILASSFTWVETLATPVADEIEVEEGVK
                     TLIEIERI"
     gene            40527..40907
                     /locus_tag="PKX59_02815"
     CDS             40527..40907
                     /locus_tag="PKX59_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6370 family protein"
                     /protein_id="HOZ89829.1"
                     /translation="MKSLFLSVLFYVLSNFTMAQVASKLASKPDAAKELITVETSCGE
                     CNFKLPGDNCDLAVRINGTAYYVDGVSISEFGHPHDDNGFCVAVRKAEVQGEVVNNRF
                     KATYFKLLPVANASENSNKQDGGK"
     gene            complement(40922..41533)
                     /gene="yihA"
                     /locus_tag="PKX59_02820"
     CDS             complement(40922..41533)
                     /gene="yihA"
                     /locus_tag="PKX59_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOZ89830.1"
                     /translation="MEISSAEFVQSNTDVKKCPEPNKPEYAFIGRSNVGKSSLINMLC
                     NKKGLAKTSSTPGKTQLINHFIINGQWFLVDLPGYGFAKTARTEREKWDGMIRNYMIY
                     RKNLMSVFVLIDIRISPQQKDLDFINWLGEKSIPFSLVFTKADKQTRAKNDFSVKQFC
                     EQLYKTWATLPPYFVTSADTKSGRQEVLDYIDSINQGFKQSGR"
     gene            41605..42576
                     /locus_tag="PKX59_02825"
     CDS             41605..42576
                     /locus_tag="PKX59_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89831.1"
                     /translation="MRNSFYILIFALVVLTSCHNDKSSDNPPCPAMEMHISGDTTVVA
                     GDSIGLMATLFTGATYSWTGPNGFIANTYNPVISESSSLSEGYYYASADINGLCKSNV
                     DSFYVTVICDRPEDVLANYITTIQTNDTIHLFAHSITDSVEYIWTGPAGFTSTQPNPI
                     ITNAIPGNEGVYTVYAHKDHCYSKPDSVVVSFNQCNPSSNTFANTLDSNYFLYSYGSA
                     NLYPNTRSITGTGLNGTSQLRATFYGNNPPTGNYIINPAHCPASGSGALAPGEVCVEF
                     SNASGTYQAVSGTVKLKALNTFDFCDAPFRLQFTTTTLFNGSGKVQF"
     gene            complement(42573..43502)
                     /locus_tag="PKX59_02830"
     CDS             complement(42573..43502)
                     /locus_tag="PKX59_02830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HOZ89832.1"
                     /translation="MQIIDPVERHLLEQELNNGRFIRDANNGQNKLYVVTAHDSPNVL
                     QEIGRVRELTFRAAGGGTGKALDLDEFDLIENCYKQLIVWNPADKEIVGGYRFIRCAD
                     AATDPHSQYKLATTELFHFTEKFIKDYFPHTIELGRSFVQPQYQPSAENRKGLFSLDN
                     LWDGLGAIAIDNPDMKYFFGKVTMYLRFNRIARDLILAFMQHYFPDKDKMVYPHHPLP
                     AENDVHDFVASLKNLPYKEGHHNLNKKVRELGENIPPLFNSYMNLSATMKTFGTALNH
                     EFGDVEETGILISIDDIYESKKARHVNTYHPAH"
     gene            43650..44216
                     /locus_tag="PKX59_02835"
     CDS             43650..44216
                     /locus_tag="PKX59_02835"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOZ89833.1"
                     /translation="MKNGLLIYCVLLTLAVAYLFYKVNSCTSNCAPAGVATAMNDGNI
                     VFVNVDTLLEHYDYYISLRESLKKKQDSIDVVLKNKGKELEGEIVNYQKRGASMTDQQ
                     RGAEEERLGHKQQQLVEYKNHLLDMLTEEEAVMQDSLHKHLVGYLKDMNKQTNFKFVF
                     GYQRGNGILLANDSLDITQSVIEGLNKK"
     gene            complement(44224..45723)
                     /locus_tag="PKX59_02840"
     CDS             complement(44224..45723)
                     /locus_tag="PKX59_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89834.1"
                     /translation="MDYRFKSMLAACFFLTATAFAQNKNDALRYSMQQTFGTTARALS
                     MGGAFGALGADFSSLSINPAGIAVYRRSEFTFSPVLQFGNSDATYLGTLTSDNKVNLG
                     VGNLGFVLAFPREKKTSGWMGTSLGFGYNRINSFSDRFAFEGINKNNSINDYFAEISN
                     GISKANLSGEDMYQYDAGLAYNSYLTNPVADSSLIDQYQGALYNGDLKQKGLITRTGS
                     QGEINISFAANYENQVYLGATVGIPYFRYSSTEVYEEYALQNTTKDSLSGKTFKNSKY
                     TQKLSTQGNGVNLKLGVIFKPTDMVRLGLAIHTPTYYEMNDQYSSKIYANFSEGYLEN
                     KSPDGRFSYNLTTPFKVITSVGLVFLDHGLISADYEYLDYSTARLNSNEYSFSDENRD
                     IKASLKNGQILKIGAEYRYKIFSFRAGTVMQSSPYEGGITYKEGNRSAVSYTGGLGIK
                     GGNFFLDLGYAYTKTSGNYAPYTLADGSAPSASINTKINRFMMTFGAKF"
     gene            complement(45752..47395)
                     /locus_tag="PKX59_02845"
     CDS             complement(45752..47395)
                     /locus_tag="PKX59_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89835.1"
                     /translation="MKSIALSAAAIFTLASCTSSQQASNAYSDDVYYSSKANKPAETV
                     QTQQQVQQPQQDVSDQSVSNQGSSTSAENRFDYNDAGTTTQDGNTYVTNNYYSDDDYY
                     DYAYSSRIRRFYRPMWNYGYYDSYYTNSYWYDYNPYDYGVSIYCGYNWWAPSSWWAPS
                     FTYFSWGYTPYNYWWSSPYSYYGYGPYHHHGWNNYSYGYWNGYNNGYWNGYYDGYYNG
                     YGYNPYYFNSYDSYSYNNTYYGPRRSTSGSGSSNAVPPRSIADSYNRALVKNGRNSMP
                     VSQSFANAMSLPTRDTKDAEVIHPRNNVNTNSPVKDSRNVETAPVRNNDAEVIHPRNN
                     VNTNSPVKDSRNVETAPARNNDAEVIHPRNNVNTNSPVKDSRNVETAPVKNSRADQFS
                     TPRNNNEVKDNSTTPAAPPVRNNSRNEVNPEYNSPRNTETVPPRNYESNPRNNNNTPA
                     ESPRRNTESNPRYNTPAESPRRNYESAPRNNRNSETAPRQEAPVRNNPPRQEHTPRQE
                     RATPAPRQERNFSEPSQQRQESRPRQESTPSRNNSRNGR"
     gene            47549..49024
                     /gene="proS"
                     /locus_tag="PKX59_02850"
     CDS             47549..49024
                     /gene="proS"
                     /locus_tag="PKX59_02850"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOZ89836.1"
                     /translation="MAKDFPTREQNYSEWYNQLVIRAGLAENSSVRGCMVIKPYGYAI
                     WERMQQQLDSMFKATGHSNAYFPLFIPKSFFSKEANHVEGFAKECAVVTHYRLKQSDD
                     GKSVVVDPDAKLEEELIVRPTSETIIWNTYRNWIQSYRDLPILLNQWANVVRWEMRTR
                     LFLRTAEFLWQEGHTAHATAEEAVEETRKMLDVYAEFAEQWLAIPVVKGVKSANERFA
                     GALDTYCIEALMQDGKALQAGTSHFLGQNFAKAFDVKFTTKQGVQDFVWATSWGVSTR
                     LMGALVMAHSDDDGLVLPPKLAPIQVVIVPIYKNDEQLKLVTDIALKIKKALTARGIA
                     VVYDDDEQAKPGWKFAEYEMKGVPVRLAIGPRDVESGTVELARRDTKEKMTVQITDIE
                     LKIENLLEQIQQNIFKKAVDFRETSTHVADSMDEFIKILDTKGGFVFAHWDGTAETEE
                     KIKEQTKATIRCIPLNNKQENGKCILTGKPSTQRVLFARAY"
     gene            49037..49723
                     /locus_tag="PKX59_02855"
     CDS             49037..49723
                     /locus_tag="PKX59_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89837.1"
                     /translation="MEPVNDKIAELIKTHFPLNCKIVEFASGHFNEIKNYLKKFVDGT
                     ASYYQPPNKRQRIKFYDTGSNQCELQLGDDTLWFHLHPNLFRISPDNPAYRYSYLRDD
                     EYRSRCAVIHVYNFLSDSLAERRSDDYGMLVARIFINYENHFMVEGRKQLGMLFNDIS
                     NDILTEQRLNDVLDQVILFCLQYDLFAQPFDQVPAMSYREISSGSFGSAVASSKQLGF
                     KLKAEDHDTE"
     gene            complement(49783..51150)
                     /locus_tag="PKX59_02860"
     CDS             complement(49783..51150)
                     /locus_tag="PKX59_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89838.1"
                     /translation="MKKIYAFVIVLLIYSFTNSVSAQSQNAIDFDGLDDQVVSVNASS
                     LIANSSQISLSFWVYPRNPVPGFPDFDGLAGFRDNTVADFYVLQYSSTAVEARFRNSA
                     GINFDILSQGLQLNTWQQFALTYDGSYLRLFRDGLITDSVSASGVISSTATSFYMGNL
                     VFSGTNFLLDGQMDEVALWNRALTPQEILCIYKNKVDATSAGLQLYYDMNTGTAGGNN
                     SGILTIPDISNHINGVLQNFSMNGPSSNFVNGIDNATIITDTICSGQTYSFNGQTLTQ
                     AGTYTATLVSSANCDSVVRLTLFSNDTTVNQNQAVLTAVQVGATYQWVDCSNNFSPIP
                     GATNQTFTATANGSYAVIISANGCTDTSSCHSVTTVGINEFSVSNWITFPNPTVSVFN
                     LVTNEIINNAEIIITDISGKQISRMTKDIKYQYAVDVSTLPDGSYIITLKSEKKSKNW
                     RLIKI"
     gene            51422..53044
                     /locus_tag="PKX59_02865"
     CDS             51422..53044
                     /locus_tag="PKX59_02865"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOZ89839.1"
                     /translation="MSAVKYVFVTGGVSSSLGKGIISASLAKLLQARGYRVTIQKLDP
                     YINVDPGTLNPYEHGECYVTNDGAETDLDLGHYERFLNVPTSQANNVTTGRIYQTVIN
                     KEREGAFLGKTVQVIPHITDEIKRRIKILGETGEYDIVITEIGGTVGDIESLPFVESI
                     RQMIWEMGSNCCVIHLTLLPYLNVTGELKTKPTQHSVKTMLEYGVQPDILVCRSEKHI
                     TADLRRKIALFCNVNVNAVIEAVDASTIYEVPLHMLKEQLDKVVLNKLKLSFKQEPDL
                     TAWKAFLGKLKHPVSEVKIGLIGKYIELKDAYKSIAEAFIHAGVANECKLNIEWIHSE
                     KIEEENVAEKLKGLSGILVAPGFGDRGIEGKIAAIKYARENKIPFLGICLGMQCAVVE
                     FARNVLGLKDAHSSEMNKETKNPVIDLMAKQKEISSKGGTMRLGEYPCALAKGSKVAQ
                     AYGKTKIYERHRHRYEFNSKYQKRFEDAGMIATGINPADNLVEIVEIKNHPWFVGVQF
                     HPEYKSTVENPHPLFVRFVKAASEFAKGAIQI"
     gene            complement(53041..53973)
                     /locus_tag="PKX59_02870"
     CDS             complement(53041..53973)
                     /locus_tag="PKX59_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89840.1"
                     /translation="MNHRFSALLLMLFISIATLSCKKEGSKYDTPVKDIEQFFDETGA
                     PVQQFSFSSSANINQTTAAGNTFMISAGSFTKTDGSAVSGNIDIKVRECVSKRDIILS
                     NLTTQSGNKFLTTGGIFYLAAYQNDVALSLSNGKTINFFLNAVFGNGQMLHRYYSSSL
                     PLENNSSDVSWTADADTQMINLLSSSSQSHYAYVMKNTGWQNAAEPVFSNSSRKKITV
                     DTGTDYETSNTAVFVSVDGSNTICKAKSVTLGVWEVENFPVNLHVTVMALAKVNDNYF
                     FSKSSVIVSTDVSIPLVMNSITLPALKTELEQLP"
     gene            54233..54997
                     /gene="surE"
                     /locus_tag="PKX59_02875"
     CDS             54233..54997
                     /gene="surE"
                     /locus_tag="PKX59_02875"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOZ89841.1"
                     /translation="MSKPTILVTNDDGITASGIRALVEAMREIGHVIVVAPDSPQSGM
                     GHAITIHNPLRLDKVDVFKGVDAWQCSGTPVDCVKLAVNKILHRKPDICVSGINHGSN
                     SSINVIYSGTMSAAMEGAIEGIPSIGFSLVNYKLDADLSAAKHYAGVITKTVLGGGLH
                     HNLLLNVNIPYLQLDEMKGLKICRQAMAKWKEEFDERLDPAQQKYYWLTGKFINPDLG
                     EDTDEWALANGFVSVVPVEFTAHHAIPVLNKLQWNV"
     gene            54990..55274
                     /locus_tag="PKX59_02880"
     CDS             54990..55274
                     /locus_tag="PKX59_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89842.1"
                     /translation="MFKKIPDTFHIGALSALALSVLAWLLISRTESYIVTSALKDYLA
                     FPRLYFIVFALQLVFLRIMMINFQKQHFARGWMFSVFIFALFVFYLIKKS"
     gene            55277..55564
                     /locus_tag="PKX59_02885"
     CDS             55277..55564
                     /locus_tag="PKX59_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89843.1"
                     /translation="MFSKDKFITGFIGGIIAPVVGFYLYYIALFRQMDLSGFYNTLVS
                     NNTLAAAISLSLLANLALFFVFDKFGKLNGSRGVIAATFIYGFYIVYLKFF"
     gene            55570..56113
                     /locus_tag="PKX59_02890"
                     /pseudo
     CDS             55570..56113
                     /locus_tag="PKX59_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918327.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
BASE COUNT        17384 a         9888 c        10807 g        17994 t
ORIGIN      
        1 ccttgcagtt ttttttacgt aacaaggaag agtctttaaa aaataaatca taagattata
       61 tggatatatt aagaaaaaca attaagaaac ttagtgatga agagtatcag gcattgttgc
      121 ttcaggtctc gggcaagaag aaaaacaagc cttattatgt cttagaagct acacgcaata
      181 acaatcattt tgatgatacg gaaatgatga agcagcttca ggttaatcca agtgcttatt
      241 atacattaaa atcccgcctc aatacaagaa tagcagaaat tctttccaag aaagttgaaa
      301 acccaattcg cgagttgatg gaagaagtgg ttcgtgttcc ggctacactt tatggaacca
      361 accgcgattt ctctatcaga gcattaaaag aaattgaaaa gcagcttaag gaatatgaca
      421 tgagcaatga gttgattaca gtttataaaa ctcttgcatt gttacacatt catcatgaag
      481 actacgaaca ttataacaaa ctttataaca aacatgttgc ttatgcattg gctatttcaa
      541 aagccgagca aatgtttttt ctgttcttta aaaaattagg aacctatcag ttaacacttg
      601 atcctgatac gttggaagaa gtcataatgc ttaaaagaaa gattgccaat attgcagaaa
      661 tgtatgattc gcatcgtttg tttgtgttat acaatattgt cagaatctat tatctgtgtt
      721 cattgccatc aaaacgacag gagttaaaat cccttgagct tgaaatagaa agtattttaa
      781 aacgcatcaa tgaaatattt aatgcatttc cgattgacac actttatagc aacattagtc
      841 cgttggtaga tatacttcac tatgagtact atcagaaaat acagaatcag gttagagcag
      901 atcattacct tagtaatgga attaacgata ctattattga ctggagtgag aaaacagcta
      961 tgcacttttt catcattcag tatctgaatt cgcgagttga aaaatttctt gctgatggtg
     1021 aaatgtaccg gttgaatgaa ttcaatcaga caattgaaaa aacttttgac ctcaatatta
     1081 acgagcctta tcattatatc tcttttaagc gatacattgc cttatgtaaa ttttatagta
     1141 aagattatca gggtgcagca aaaactatat acgatttaag aaataatatt tctttgaagc
     1201 agttcactca taccgatgtt gacagcaaac tctttcaggg tttgtgttat tgcctgatgg
     1261 gtgaagatag ccttgcacaa caaatgattt caagtattaa acgtcagatt gccgactttg
     1321 aacatgagtt tgagtcaaca acaatattta ttaagattct taaaacagca ttaaagcctt
     1381 ctgacttcag aagaaaaatt gtgcgcatca acgagttgct gattaagttt gaagaagcaa
     1441 ataatggtga taaaccaatt ctgaagtata tcaaaattga tgatgcattg attcggaaaa
     1501 tgacaaatcc gattaaagat taaaacaaag gcggcagaaa tggccgcctt ttatttttgt
     1561 gctttttctg acaaatttct gttactttta tcatctgata ctatcaaatg gatattgaaa
     1621 aattcaggaa ctattgtttg tcgaagcaag aaacaacaga gagttttcct tttgacgaag
     1681 acacacttgt gtttaaggtt gccggaaaaa tgtttgcttt agtttcgtta tcagaaaaac
     1741 caatgacggt aaacctcaaa atggatcctg aaattatatc agaatggcgt gatcgctatt
     1801 ccgaaattac accgggattt cacatgaata agcaacattg gaattctgtt atttttaatg
     1861 gctccattcc tcttgcagaa ttaaaatggc ttattgatca cagctattct gaagtagtta
     1921 aaaaattacc caaaaaagta agagagcgat ttacaattta gtgttattca gaaattacac
     1981 tttcttatca tcaaaattta taattagccg ctcaacaggt ttgttgtttt ttatatgttg
     2041 ttctactatt tcatcaacat cctgtagctg aacattacca taaaaaatac cttcgggata
     2101 aacaactact gatggaccat agtcgcaggc atctaaacaa cctgaccttt gtgcacgaat
     2161 tggcaccggc aactgtaatt ctttaatttt tttcttaaat gctgcaacca atgcaagacc
     2221 atgtacttca ccacagcatt tacgttcacc ttctttacgc tgattagtac aaacaaaaat
     2281 atgtttttga aacctcataa tgcaaattaa gataattgtg ttcagaattc aaactctatt
     2341 agtccaattc aaattcaatg cttaattttg ctgttaaata ttacagagta tgaacgacat
     2401 agcaaaaaag ataattgata cgatgcaatc gcacatggac aaatccattt cgcatcttga
     2461 gactgaatta gcaaaaatca gagccggtaa agcaaatcca cagatgctcg aatctattca
     2521 tgttgattac tatggttcgc aaacgccatt acatcaggtt gccaatgtca atactccgga
     2581 tgcccgcact attgtaatcc aaccatggga aaagggtatg ttaggtccca ttgaaaaagc
     2641 aattcagatt gctaatctag gatttaatcc gcagaatgac ggttctatca ttcgtattgc
     2701 agtgccacca ttaactgaag aaagaagaaa agatttggtt aaacgttgta aagccgaagg
     2761 tgagaatgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaga agccaatgat
     2821 gcaattaaga aagaattgaa gaaaggcatc actgaagacg aagcacgcga ggcagaaaca
     2881 aaaattcagc aaacaactga tggctttatt tcaagagtgg ataaacatct tgaaacaaaa
     2941 gagaaagaag taatgactgt ttaacgcctt ctaaatagtc agtagttact attgctcctt
     3001 aggagtctga gaaatttcgt ccaactgttt tttaatacgc tttatttctt ctttggcatg
     3061 attgatttta tcttcaaact ccttgcgtac agcatctgca ttttttgagc gtgcaaaaaa
     3121 gccaagatta ttttcccatg tattaatttc tccggtaagt tgattaatct tatgctttaa
     3181 atcattcttt tcattacccg gtttgccgct tctaaaagat ggtttatgtc ctgatgtaaa
     3241 tcgctctttg ttatgctgtc ttattgtttg aaaatgtttg tcaattgctg ctttgaactt
     3301 tgcataaatt gtgtctttgt gttcaagagg taccatacca atctcactcc actgacgttg
     3361 aaaatctttt aactgctcaa cagactcatc aatgttttca ccaagtgtaa actgttcagc
     3421 tttttcaatg agtgcatttt tcttgtcgaa gttttcttta tagtctgatt caagtgaagt
     3481 gtaatgtgca ttttttcttt caaaaaatga atcacatgct tttctgaatc tttcccagat
     3541 tttctgattg tttctatcac cggcaaatcc ggtttttttc cactcagcct gaagtctttt
     3601 taattcttct gttgtttttt tccagtcggt gttttctatc aatgcttctg cctgaataca
     3661 cagttcgttt ttcaattgca gattggcagc atgttctttt tttagtgagg tgaaaaaatc
     3721 atttttacga acaaaaaaac catcacacaa tcctctgaat tttgcccaaa cctcatcatt
     3781 agatttcttg tcggcaaagc caactttttt ccattcgttc tgaacttcaa caagcatttt
     3841 gcttttttcc atccattcag atgctttaag tggttcgtct ttaagtaact cttctgtttt
     3901 ggtgataagt agattttttg cagttaaatt ttcagcatag cgttgcttta gctgttcaaa
     3961 atattctttt cttctttcat aaaccttatc acttgctgct ttaaatcgtt cccaaatttc
     4021 attgcttttt tcacgaccta catgaccggt ttcgcgccat ttgttttgta aattatgcaa
     4081 tgtctgcaga gctgaattta atgatggctc attaatcagg tcttctgcct tttcgcagag
     4141 ctcaatttta gcttcaaggt tctttttaaa atcaagttcc tgaagttcac ggttaatttt
     4201 caggttttca taaaacttat ctgtgtaaag tttgtaggtc atccacaaat cattcaactg
     4261 acctgaagga acaggccctg ttgcacgcca ctttgcctga agttcgtgaa actgatcgaa
     4321 tgcgcgttgc atgttttcct cattctgaat aaggtttttc atggcaacaa gtatttcctg
     4381 ctttacacta aaattatttt ctctgagttt ttcctgttgt tcggcatact cagctttgcg
     4441 tttattatat tttttaagtg ctgcctgaaa acgatcatct gattgtgtgt agttatattt
     4501 gaaatcttct ttaacaccac cttcttccaa aaaagttgat agtgcctgtt ctttttctga
     4561 agattgaatt tgttcaaatg catttttcag tgcattgatt ttacttttaa cggcagcaac
     4621 atcaccttca gcaaatcctt cagcaatttt aacaagttct tccttggtta agtgattgta
     4681 attttcttcc tgctcaggca aggtttcagg atgttcttct ggcaatgttg caagatgttc
     4741 ctcctgcaag ggttgtgtaa tagatgcatt ttccgattga atctcaccag cctgctgatt
     4801 ttcatccgca tgaaaatcat tgtttacttc tgtcatgata ctaattaata taatattaaa
     4861 atcaaacaat gaagccttcc attgtagaag gctttatcag actacaaatg taatacaatt
     4921 ttccatttct gtgcaagaag ttattgctct aaaaaagcct ttttgaaaat tagatttatt
     4981 ccacattgta attccataaa aaccattttt gccggctaca cgtataaaaa atggtaaatt
     5041 tggagcgtta ttgaattatg ttaaaacaaa acttatcaca aaaactttta cagaaacttt
     5101 ctcctcagca aattcagctg atgaaattgc ttcaattgcc aactattgca ttagaacagc
     5161 ggataaaaga ggaaatggaa gtaaaccctg cattagaaga aggtgcgccc gaagatgtgg
     5221 atgaagatac acaacaggat gaggacgaag tgcctgaatt ggaagagggg gtagaagcca
     5281 atgaaggcga ggagaataaa aaagacgagt ttgataacac ttctgatgat gatataaaag
     5341 aaagaacaga tgatgatttt gatgtggatg attatatcac tgatgaggat ataccatatt
     5401 ataaaacatc tgccaataat acttcgcccg atgatgaacg cacagaggtt ccttatgggt
     5461 cttcagtaac atttcaggaa acattactgg cacagttggg tatggttgcc ctaaacgaaa
     5521 aacaaatgca gatggctaag catctgatcg gcagtttgga tgaagatgga tacttacgca
     5581 gagatttgaa ttctattgta aatgatttgg catttacaca gaatatagat actaatgccg
     5641 atgaactttt acaagtgtta caagtgatac agacatttga tccacccggt gttggcgctc
     5701 gtaatttgca agagtgtctt ttgcttcaat tgcagcgaaa atctaatagt aagtcggtta
     5761 atagggcaat tgaaattatt aaaaattaca tggatgagtt ctcaaaaaag cattttgaaa
     5821 aaattgccag attgatgaac attcctgatg agtatttgaa ggaggccatg aatgaaattt
     5881 taaaattaaa tccacgtccg gggggtgata tttctgcaga aacaaaatct gttcaacata
     5941 ttgttcccga ttttatcatt acaaataacg tgggtcagct tgaattgaca ttgaactcac
     6001 gcaatgcccc agatttgcga attaaccgcg agtacaatga aatgatggaa ggctatgcca
     6061 agagtaaaaa gcaagatcgt tcacagaaag aggctatcct ttatctgaaa caaaaaattg
     6121 attcagcaaa atggtttatt gatgccatca gacagcgaca ggcaacactg cttcatacca
     6181 tgcaagctat catgaactat caaaaagaat atttcctgga aggcgatgaa cgacttttaa
     6241 agccaatgat tttaaaagac attgcagatg ttgtcgggct tgatatttca actgtttcac
     6301 gagtagcaaa cagtaaatat gtgcaaacac cattcggcac atttctgata aaaacttttt
     6361 tctcagaagg tatggcaaca gactcaggcg aggaagtaag tacgcgcgaa gtaaaaaaaa
     6421 ttctgtcaga tgctattgaa ggtgaagaca agaaaaaacc attgccggac gacagattgg
     6481 caaaaatttt aaaagacaaa ggttataatg tggcgcgtag aaccattgct aaatatcgcg
     6541 agcaactaaa tattcctgtt gccaggctac gtaaagagtt ttaaaaatgc tgaaccgctt
     6601 tttttcagtc gtcacttatc taacccatcc tttacttata cctacttatt tattactgat
     6661 actttttaat tcggacacat atctgtcata taccctttcg cctgcattac agcgaataat
     6721 tttatcagcg gtttttgtcc tcacctgtct gatgcctttt ttatcatcgg tattattact
     6781 taaaaagcaa ggtgtgcagg gtttgttgat ggaggacagg cagagcagaa caatacccta
     6841 tttttttaca cttatatatt atgtagcttg ttattacttt ttgttgaaac ttccggtttc
     6901 atcggttata aaaattctta ttctggcagc agcatgttgt atagcacttg catttactat
     6961 taattttttc tggaaaataa gtattcatct cattggatta ggaggtatgg caggtgcttt
     7021 ttatgtactg tcgctattgt taagtagtga atttattctg ccgtttgtac tatcaatcat
     7081 tcttgccgga ctcaccgctt ctgcccgttt gatgaaaaat gcacactctc caagtcagtt
     7141 atattttggg tttattgcag gttttgtttg tgagtatatt tttatcatgt actcattaaa
     7201 ataaactaca accttctaaa attcatatat aaaatctcaa attatttaaa ttcgtttttt
     7261 aattttttat accaccgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngact
     7321 aaaagtgtat atcaaaaact ggaaaaacca tcagcaactg atcctcagta gtaagtgtac
     7381 atgttaatga agagtgtaag ccctcttgta cattatgaaa ccggtaagat gattttaaat
     7441 tctgtgcctt ctagttcttt tgaagttact tcaatggtgc ctttatgtgt attaataata
     7501 tcaaagctta aactcagtcc taaacctgtt cctttacccg tgggtttagt tgtaaaaaat
     7561 ggttcgaata ttttttgttt catcttttct gaaattcctc ttccattatc acgaacagaa
     7621 ataaagattt tccgattgga tttttgcctt gatgtttgag ggattgaagc cgttgtaata
     7681 accaattgtg gtttaaaatt ttcaccttta agttttaaac gttcgcattc gtataattca
     7741 tcaaaagcat tatcaatcag gttaagtacc accctcgaaa tgtcctgacc attgcaaatg
     7801 acttgaggca agttcggatc aaaattttta atcagatcac aattaaaatt ctgcaagatg
     7861 gcacgtttac catgataagc aagatctaaa tattcatcac acacagagtt tatgttagtc
     7921 ttttgccggt cttctgtatt ggttcgagaa tgttccaaca ttctttttac aatattatcg
     7981 gcccggtggc catgctctgt aattttaaca aggttttcgt gaagcaaatt catggcttca
     8041 tccttatctt gtactgtttg agcttctttt aattctataa gtatttcatt gctcagactt
     8101 gcaaaattcg tgacaaaatt caacggattt tttatttcat gtgcaatgcc tgccgttaaa
     8161 acacccagtg aagcaagttt ttcctgttgc accaattgct gttgcgtggc ttgtaaatgc
     8221 tgatatgctt ccttcaattg caaatttgtt ttttgtttaa accgatacct gtgaaacagt
     8281 agcattaata aaagcaatag aattgaagca cctgcaatca tcgaatttct gaaaactctt
     8341 tgtgcaacag catttttttc ttgttcagct tttaagagtg catctttctt attattttca
     8401 tattgaattt gttgcagcag aatttttttg gtgttttcgc ggttataggt actatccctc
     8461 aggatgatat aatttttata gttgttatat gcgccttgcc agtcactggt tgcactgtca
     8521 agtagggcaa cagcatgata gtattccgtt actggtactt ttgaatctaa tttctctgca
     8581 agagtccaag cttcatcaag gtttttcttt gcagaatcat aattacctaa tatagtttga
     8641 caaatgctta gtttgcttaa agcagaggca agatcaaaat agtcagatac tactctaaaa
     8701 gaattagccg ctgcctgaaa gaactgtatt gctttttgat aattctgttt ctgaaagtaa
     8761 acatcgccca aactttccga agcagttgcc atggcaaaat cattactaat acttttggca
     8821 atttcatacg ccttattata ataacttatg gcctctgttg tattacccaa ttgttgatat
     8881 gtctggccga cactggtata tgctactgtc agattaataa tatcctttgc tttatcaaat
     8941 gctttgatgg cggacaaata aagttttaat gcttcagaat aatttccttg agtggaatac
     9001 actccggcaa tattactcag tacaatagct tcgccatact tatcaccaat agcttcaaat
     9061 attttaagtg ctttataatt tgcagcaaca ttctccacat aattaccctg actttcgtac
     9121 acaaaaccaa ataaaaggta gtttcctccc attttactgc tgtctctaag cttttcataa
     9181 taagcacctg cttttttaaa ataggataag gcatcttcat atctgccgcc atctgcataa
     9241 tcctgaccaa taaaggttag caattccgct atttgtggtt catcatttct gctttcataa
     9301 tattttaaaa catcaaaatg acattttaaa gccatatcaa aaaaaccttt acgccaataa
     9361 accctgccaa ctaaagcaac taattttttt gttcctggtt cccatttcaa actgtcggca
     9421 aattgaagtg cgggtttact ataaaaaagg gcagaatcgg tattgaaatc taataaataa
     9481 tagattaact tttggtaaag attaagctta tgtgtatccg cttttgcatg attcaatgaa
     9541 tacttaaggg aatctaagta tttgttttgt tgtgccaaac acatattggt ttgtatcaaa
     9601 caacacacta acagaatcgc aacaatttgg tttttcataa agacgattca ccaaatttaa
     9661 aggtctgttg tgtacaaatt acttttttgc aatttttttt ttggaattga agtagggaaa
     9721 acaactgtta cagaaatagt ttttctaaaa ttccggtata agcgaaataa tagtttgata
     9781 cagtaatact attgtggaat gaataaaatc tgaattagaa tatttaaatt catctatagg
     9841 acaattttgg cacagcagtt gaatttcatc aggtaaatta gaaagtgagg ttcattatga
     9901 aaaaactatt tactactctc gtcattttaa gtttagctgt tgttgttcag gcagcacatc
     9961 ctttacaatc tgcattggca attacttctg ttgatggaag tcttctgacc gtatcaatag
    10021 acagagcacc atttagcaat tcttcaacaa atatttatac agatggatta cgtgcgggaa
    10081 accattatat taaagttatg cgacttgccg gacaccctcg tcaccctgtc tatcgaactg
    10141 tatatgccgg ctatgtattt atacctgaag catcagaagt gatagcagag gttaaccgta
    10201 acagatttcg tatcaccgga agcagacctt ttgattatgc gcctcaggta tatgaagatc
    10261 cacactatta cccacaacat cttcgttgcg aagtagatga ttatgagttc aatcagatga
    10321 tacagtcatt gggtaattat aattttgaaa gtactcggat gacagttgca aaacagttaa
    10381 tcaataataa ctattttaac tcacgtcagg ttgggcgatt aatgcaacaa atgacctttg
    10441 agtccagcaa acttgaattg gcaaaatatg cttattcaaa aacattggac aaaaacaatt
    10501 attttttagt caacgaccag tttagttttg aaagtagcat tgccgagttg gcagattata
    10561 ttgcattgcg taactaaaag gaataaataa actgcagtga aaaggtgtca gaaatggcac
    10621 cttttttatt cgtctatttt tattttaata tcatccttaa ctataaaagc tgacggataa
    10681 taagtctgaa gctcttttag tcttttttgt gcatccagac gagtgcggaa atcgccaacc
    10741 cgtaatttaa agttcggctg ttgataaagt acataggctg ctgtttgagg ataaagcttt
    10801 ataaattctg tcctaaactc ataggctttg tcacgttgcg acccaaaata aagttgaatg
    10861 cggtagcctt gtgaataggg tttagccaga ttataggcaa tatgcttatt tataagtaca
    10921 tgagtcagac tatcatcact ggtctgagcc ttgatttgta aatgaattag aatgcaacat
    10981 ataataaaga aggcactctt tacaatttta atcatggctt caatattaca gattatatgt
    11041 aaaacgggct gcaaaaacag cctaaaattg aggggcatag taaaaaaaaa cctaaaaggc
    11101 tgttatttag actaaatttt aatttgaaaa ttttatgaat taaataaggt aagttataaa
    11161 tttgcgcgct ccaaactgga attttacgga attattgcag tttgaactaa attataaatt
    11221 gatacgaaaa caaaaacaat ttatgcacca aaagctcact aataaccttc ttgtcaagtg
    11281 taaggggttg tttttcatgc tcctgatatt gggattttca gcaggagtta aagcacagga
    11341 tggtaaagca ctttttgatg ccaactgtaa atcatgccac gcaatgaatg atgttgtcat
    11401 tggtccggca cttaaggatg tacataaaaa attcagtgca gaatggctac ataaatggat
    11461 aaagaactcg caagcattaa taaaagctgg agataaggat gccgtagcag ttttcaatga
    11521 gtataataaa atggttatgc ctaatcagaa tctttctgat gctgaaatag atgcagtatt
    11581 ggcatacatt ggtaaagaaa gtgagcaggc acctgtagct gctgcaccaa cagcacctgc
    11641 acccggaaca gttgctcctg aaaaagataa ctctacttta tattgggtaa tagctattat
    11701 cctgattctg ttagcccttg cagcaacact taacaaaatt agaaagggtc ttgagtatgc
    11761 agtacgcgaa aaagaaggca ttccacaccc cccacactta gagccaaaag aagcacgtag
    11821 tttatggatt agtaagaata aaaaactgat tgcagttgta ttacttttat tagttgtctt
    11881 aggtagtgtt aaaggttggt atgctttaaa ggatattggt gtatatacca attatgagcc
    11941 tgagcaacct attcattttt cacataaact gcatgcagga gataataaaa tagcctgtct
    12001 atactgtcat agtggtgcag acaaaagccg tcatgccaat attccatcag caagtacctg
    12061 tatgaattgt cacaaatatg tgaaagggtc ctgtgtatgg cgataaagaa atcaagaaaa
    12121 tttatgcagc cctcgattgg gatggttcta aatttggaac caatcaaaaa ccaatacaat
    12181 ggatcagagt acatagttta cctgcattgg cgtattttaa tcatgctcag cacgttactg
    12241 ttggaaaaat cgaatgtcag acttgtcacg gtcctgttca ggagatggat gtggtgaaac
    12301 aattctcacc attaactatg ggttggtgta ttaattgcca ccgcgaaaca gaggtgaaaa
    12361 tggatggtaa tggatattat gacgaagtgc ataaaacatt agcagccaag tacggtaaag
    12421 atgctaaact tacagttgac aaaattggcg gtcttgattg tgcccgctgt cactattaat
    12481 caaaacacta aaagctgaat ctgttcattc aaattaaaaa cattcggcca caaacttttt
    12541 aaataatata tggaaaaacg atattggaaa ggtgtagaag aactcagaaa tgatgcagag
    12601 tttgttcgtc ttaaaaacaa tgagtttttt gaagaaattc ctgttgatga agttcttggc
    12661 aaaaaagctg aaagcaaaga tgccacacca cgcagagatt ttctcaaatt tttaggtttt
    12721 ggtgttgcag ccgcttcaat ggcagcatgc gaagctcctg ttagaaaaac gattccttac
    12781 ctgataagcc cggagaactt agttcccgga attccaaatt attatgcatc aacatttttt
    12841 gatggccatg attatgcaag tgtcgttgta aaaactcgtg aaggtcgccc tattaaggtt
    12901 gatggtaatg aattgtcacc acttacaaaa ggtggtgcta atgcaagagt tcaagcttca
    12961 gttttagatt tgtacgatag cgaaagaatt aaaagcccaa ttgctaaagg aaaagcagca
    13021 agttgggata cagtaaataa agaaattaaa gaaaaacttg cagctgctaa tacaaaacag
    13081 gcaaaaattc gcatagtttc ttcaaccatc attagcccaa caacaaagaa ggctattgaa
    13141 agttttacca caaaatatcc tactgctaaa ttggttactt atgatgccat gtcacaatat
    13201 ggtataataa aagcacatca attagcattt ggtaaagcag cacttccaaa ttatagcttt
    13261 gataaagctg atgtaatagt aagttttgga gctgactttt tgaataactg gattgcacca
    13321 attgaatatg cacgtcagta tgcacagaca agaaagttag atgatggaaa gaaaaccatg
    13381 tctaaacaca ttcagtttga agcagcactt tctttaacgg gtagtaatgc tgataaccga
    13441 tataaattaa aagcaagtca gacaggaaat gctttattgt cattatataa tgcaatagct
    13501 tcaaaagcag gtgctgcaac agtaagtgcc ggcaagaccg atgcagatgc tgctattaac
    13561 agcacagcaa acgatttatg gaatgcaaaa ggtaagtcgt tggtagtatg tggaactaat
    13621 aatgccaatg atcaattagt tgttgctgcc attaacaaca tacttggtaa ttatggagca
    13681 acaatcagta tagccaaaca tagtaatgtt cgtcagggaa atgatgaagc tatggctgaa
    13741 ttgcttcgcg aaatgaatgc aggtgaagta ggagtagtaa ttttttacaa tgcaaaccca
    13801 ttatataact atcctgatac tactgcattt acttctgcat taaacaaagc agaagtaaaa
    13861 atttcactat ctgaccgtaa tgacgagaca gcaataaaat gcgattatat ttgtcctgat
    13921 tctcattatc ttgaatcatg gaatgatgca gagcctgtgc gcaatatgtt cagtttggtg
    13981 cagccaacaa ttcaaaaact gttcgataca aaacaatcac aagaactatt cttgttatgg
    14041 agcgatgcac ctgtaacaga ctatcatgag tttctgatgc aaaactggag acaaggtttt
    14101 ctggcaacag caacaggaat ggcatcagta cctgcatggg aaaaagtttt acaggaaggt
    14161 gtttggaatg gtgaagttac agagagcgag cctgcatttg ttggaaacgt aaatgatgca
    14221 gcaagtgcaa ttggagctag aaaatcaggc ggtcttgata ttgtagttta tgaaaaagcc
    14281 ggtataggta atggacgtca tgcaaacaat ccatggctcc aagaattacc tgatccggtt
    14341 tctaaaatat gctgggataa ttattttgcc atagcaccat ccatggcaaa agaaaaaggt
    14401 ctggtacaag gaaatattat tgaagttaag tcaggaaatg taacacttaa aggtccggtt
    14461 ttactccaac ccggtcaggc aaaagataca attgcagtag ctgccggtta tggtagaaca
    14521 aacagtggta aagccgggaa taaagtaggt atgaatgcat atcctttact gactttttca
    14581 gaaggaacat tcaactatac caatagtgga gcaagtttaa ataaaacagt tgaggctgat
    14641 tatacacttg ctacaacaca atctcaccac accatgatgg gtcgtgctat tgttaaggaa
    14701 accacacttg aagagtatat taaagatcca aaggcgggta atgaagaaga gaaatttcat
    14761 tcttataaag gaaaagtttc agctaaagaa ttagacctct gggcaacaaa agatcatccg
    14821 ggcttcgatc gcccgattaa tgcatggggt atgtctattg acttaaactc atgtattggt
    14881 tgtggtgcct gcgtagtagc atgtacagca gaaaataatg ttcctgtagt aggcaaagaa
    14941 gaaatacaac gcagccgcga gatgcactgg attcgtattg atcgctatta taccagcgat
    15001 atggataaag aaaaagcaca tgagcagggt gtaggtaagt taagcatgta tggttttatg
    15061 gaagagcctg cagagaatcc tgaaattgta tttcagcctg taatgtgtca gcattgtaat
    15121 catgcacctt gtgaaacggt gtgtccggtt gctgcaacaa cacatagtga agacggattg
    15181 aatatgatgg cctataaccg ttgtgtagga acacgttatt gtgccaacaa ctgcccctat
    15241 aaagttagac gttttaactg gtttaaatat tctgataatc cacaatttga ctataacatg
    15301 aataatgatt atggcaaaat ggtgttgaat cctgatgtaa ttgtacgctc acgtggagta
    15361 atggaaaaat gctcaatgtg tgttcagcgt attcaatatg gtaagttaga agcaaagaaa
    15421 gcaggccatc cggtaaaaga tggagctata aatacagcat gtgcacaaac ttgtccgacc
    15481 aatgcaatta cttttggtga ttataatgat aagtcaagtc gtttggtaaa acaaagcggt
    15541 agcgaacgca tgtatcattt gcttgaagaa cttaatgttc aaccttcaat taattattta
    15601 actaaggtgc gaaacactac aggcaaaaca aataacagtc ataaagcata aataaaatta
    15661 atcgaactaa tagaaatttt tcaacaatat gtcgaatgta gaatcaccgc ttaggccgac
    15721 actcatcgaa ggagataaaa cgcttcatga tattaccgaa gatgtttgca gacctataga
    15781 atctaaaccc aacaaatact ggtggatggt atttagtgtt gcctgtgctg cactgttatg
    15841 gtggattatt tgtgtagcct atacagttgg aacaggactt ggtgtttggg gtctgaataa
    15901 aacagttgga tgggcttggg atattaccaa cttcgtttgg tgggtaggta ttggtcacgc
    15961 agggactttg atttctgcaa tcttattatt gtttcgtcaa aaatggaggc tatcaattaa
    16021 ccgttctgca gaagcaatga ccatatttgc cgttatctgt gctgcctcat ttattgtagc
    16081 acacatgggt cgcccttggg ttgcatattg gccacttcca tttgcaaacc aattcggttc
    16141 attgtgggta aactttaatt ctccgttggt atgggacgta tttgcaatct cgacttattt
    16201 ctctgtatca ttagttttct ggtacacagg attgattcct gactttgctg cagttcgtga
    16261 tcgtgcaaaa actaaagctc gtaaattctg gtattcagcc ttaagtatgg gatgggttgg
    16321 ttcagctaaa aactggcaac gttttgaaga ggtttcttta gttcttgcag gtatttcaac
    16381 accactggtt ctttctgttc actctgtggt atcaatggac tttgcaacag cattggttcc
    16441 gggttggcat acaacaattt tcccaccata tttcgtagcc ggagctattt tttcagggtt
    16501 tgcaatggtg cagacactgc tgataattac acgtaaagta ttaaacttag aagaatatat
    16561 tacaatttat catattgaca tgatgaatag gattattctt attacagggt ctatagtagg
    16621 ttgtgcttat ctgacagaat ttttcattgc ctggtattca ggtgtagaat ttgaacaata
    16681 tgcatttatc aatcgtgcaa caggaccata ttggtgggct tattggagca tgatgacttg
    16741 taacgtgatt tcacctcaat tgatgtggag tagtaaactc agaagaaatc ttttcgtaac
    16801 tttcttctta tctattattg taaacatagg aatgtggttc gagcgttttg tgattatcgt
    16861 aacttcatta cacagagact ttgttccttc aagctggaca atgtttcatc ctacttgggt
    16921 agagattggt ctctttattg gttcgatagg tttgttcttt gttcccttct tactctttac
    16981 acgatatttc cctgttatag caatgagcga attaaaaaca attttgaaat catcaggctc
    17041 tcaatcaaag aaaggagtac aacatcatga ctaaacaact gcatggcatt tttaatgatg
    17101 atgaagttct tttaaatgca attaaagaac taaagtcaaa aggtattaaa tgtactaatg
    17161 cctattcgcc ttttcctgta cacggacttg atagtgcctt aggtcttaaa cgaacaagaa
    17221 tttccatagc ctctttttta tatggtgcta caggaacatg cttggcactt ttaatgatgt
    17281 ggtacatgaa tatttgggat tggccaatgg atataggtgg aaagccaagt tggaacttat
    17341 ataagaatat ccctgcattt attcccataa cttttgagtt tacagtttta tgtgcagcac
    17401 atggcatggt gattactttt tatctgcgaa gcaaattatt acctggagtt actcctaatg
    17461 ttattcatga tagaatgcat gacgatcatt ttgtattaac cgttgaagga aaagtaagtg
    17521 atgaaacaac tattcgcgaa tcattgaaac aagttggtgc agtggaaata agatcataaa
    17581 gaattaaaac ggatgaaaaa cacacacata tattttaatg taattattgg aggcatgata
    17641 ttgcttagtg cttgtggtga taaacaatca ccgggcttgg agtatatgcc tgatatgtat
    17701 cgtactccag cggtggtagc ttattctcca tcacacatca caaaagatag tgtttctgca
    17761 ttggcacccg ttgccggaac tgttgcaaga ggtcagtatg ttgactttat ttatcctaac
    17821 accaatgaag gatatgaggc tgcaggctta gagttgaaga acccttttca accaacagca
    17881 gaaaatatgg agcgtggtaa aacagaattt gatacctatt gtgcccactg tcatggggag
    17941 aagggtgatg gtaaaggatg gctaaaaatt aaaggagatc catttcctgt gccatcttat
    18001 acaagcgcag cgattaaaga cttacctgat ggaaaaatgt ttttctctat tacttacggt
    18061 aaaaacctta tgggatcaca tgcagcacaa gttagtgctg acgatcgctg gaaaattata
    18121 ttatacatta accatttaca gggagacgaa ccaggcacag ttgtagctga cagttcggct
    18181 actgcacaaa acaaataaca taacgcagcc tatttaagat aatactatgc aggacaatct
    18241 cacatatacc ttttcaagta agctgaaaac cctttcgttt attctcatgg cgataggtgc
    18301 aatttcaatc ggaataagct ttgctaccaa tccggaccaa acatgggcaa acctcatgca
    18361 caacaacttt tacttcttaa ccatagcatt aggaggtctg tttttttatg cagtacaata
    18421 tgcagcagaa gtaggttgga gtgtttacat cattagaatt ccattagcga tgtctcaata
    18481 tctgtggatt agcggtgtta ttatgctcat tttgtttttt gctgccggac atcatatttg
    18541 gcattggaca catcatgagt tagttatgga aacattgcct gatggaaagc ctaatccgga
    18601 ctatgatgaa attttagcag gaaaaagtgc ataccttaac gttccattct ttttaattag
    18661 aatggtagca tactttgtaa tatggtatgg ctttgcagtt ttgttacgta agcatgcaat
    18721 gaaagaagat ttatcaggtg caatagaaca tcataaccgc agtcgcaaac tcagtattat
    18781 atttcttgta ctttttgctg ttacatcatc tacctctgcc tgggattttg taatgtcaat
    18841 agactcacat tggtttagta cattatatgg ctggtacact ttttcaggaa tttttgtaag
    18901 ctcattaggt gtaatcgctt tgattattgt ttatctacgc agtcagggat atctacatca
    18961 tttaaacgaa aatcacttac atgatgtagg taagtttatt tttgccttca gcatattttg
    19021 gacctattta tggtttgctc aattcatgct catttggtat gctaaccttc cagaagaagt
    19081 aacctatttt atggaaagac aagattatta taaaacactt ttcgtaacta atttcttagt
    19141 caacttcatt cttccatttg tagttttaat gactcgtgat gcaaaaagaa aattgcgttt
    19201 attggcaata ttaggctctg taatttttat tggccattgg atagatacat atctgatggt
    19261 agttcccgga ataatgcatg aaaaatggac aattggagta ttggaaattg gaacaaccct
    19321 tgggtttatg ggcatgttta tctttaccgt atttactgca cttaccaaat tatcacttct
    19381 tcctcaaaaa catccaatgc ttcaggagag cgaacatttt acattgtaat ttttaaaata
    19441 gacattaaca caaatcagta atttcaagac atgacactat taatcacaat atcaatctta
    19501 cttggcattc ttgttttagt cagactgatg actattgtac aattatcctc agaactaact
    19561 ggtagcagcg attctcaaga agaacagatt agacacaata attcaaatgg atttggattt
    19621 attgtattta tggtcttagg tctaggttta tttatctata tgaccataga ctattccaaa
    19681 cacctgcttc ctgtatctgc ttctgaacat ggtgttggtt tagataaact tttaaatatt
    19741 aattgggcaa tcattggtgt agtattcttt attacacaga tagccttatt ttatttttca
    19801 tacaagtatc gtgacaataa aaagcatgaa gcttactttt atcctgaaaa ctataaactg
    19861 gaattaatct ggacaattgt tccaaccatt gttttaacta cattgatcat taccggtctg
    19921 aaacaatgga atggtatctt ttctgaaaaa gaaaacagta tgaatgttca ggtttatggt
    19981 tatcagtttg cttggataac ccgctatagc ggacctgata atacacttgg aaaatcaaac
    20041 tacaaactca ttactgatga taatccttta ggtttagatg caagtgatcc tgccgtaaaa
    20101 gacgatatct atacaacagg aaatgaaatg cacatacctc ttggacaaga gttgaaattt
    20161 cagtttaatg cacgtgatgt aattcacagt gcatattttc cacacttcag agcacaaatg
    20221 aacttggtgc cggggatgaa tacatttttc tcgtttaaac caacaattac tacagcagaa
    20281 atgcgcaaga ttaccggtaa tgataagttt gactatgtat tgctttgtaa caaaatatgt
    20341 ggtgttgcac attacacaat gaaaatgaaa gtagtagttg acacgcctgc agattttaaa
    20401 gcatggttga aatcacagaa aaaagcttct gaagtgaaag cagcacctcc ggtaagtgca
    20461 ttaaacagta ctgtaaaagg cagtaactca atttaaattt gatattttct tttgttcgat
    20521 ataatttagg ctaagaataa aattacaatg gaagtaaaac aaacagcagg tgttgcagta
    20581 gagcatcacg atgatcatac acatgagcac gatcatcacg aaacatttct gacaaagtat
    20641 attttcagtc aggatcataa aaccattgca aagcaatatt taattacggc aattatcatg
    20701 gcattcttcg gaatgtatat gtcattaata tttcgtttgc aattaggatt tccggaaacc
    20761 aaatttgctt ttcttgaaag tttgattgga aaatggggta aaggtggaag attggatccc
    20821 ggcttttata tggcacttgt tacgatacat ggaaccatca tggtattttt tgtattgaca
    20881 ggtggattga gcggaacttt cagtaacctg cttattccat tacaagttgg tgcacgtgat
    20941 atggcatcgg gctttttaaa tatgctgagc ttttggtttt tcttcctttc ttgtgcagta
    21001 atgacatatt ccgtttatat tgaaacaggc ccagcagcag gaggatggac agtatatcca
    21061 ccattgagtg ccttaccgca agcaatgcct ggctctggac ttggaatgac gctatggttg
    21121 gtgagtatgg ttttctttat aattcagtct ttgcttggtg gtattaatta tatctgtact
    21181 gttatcaatc ttcgaacaaa aggaatgagt atgacaaggc ttccattaac agtatgggca
    21241 ttttttatta cagcaattct tggtgtattg acattcccag ttctttttgc agcggcttta
    21301 cttttggtct tcgacagaag ttttggcaca agtttttatc tttcagatat ttttattgga
    21361 ggtgtagcat tacctcaatc aggtggaagt cctgtattgt atgaacactt gttctggttc
    21421 ttaggtcacc ccgaagtata tattatcatt cttcctgcat ttggtttgac atctgaagta
    21481 ttgtcaacaa tgggacgtaa accaatattt ggttataaag caatggttgg ttcattaatg
    21541 gcaattgcat ttttatcatg tattgtttgg ggacaccaca tgtttgtaac agggatgaat
    21601 cctttccttg gtgcagtgtt tacatttaca actttactag ttgcaattcc ttcggctgta
    21661 aaagtgttta attatttggc tacactatgg agagcaaaaa tacaactcac accagccatg
    21721 tgttttgcta ttggattagt gtcattattt atttctggtg gattgacagg aattatcctt
    21781 ggtgattctg cattagacat caatgttcat gacacctact ttgttgttgc ccacttccac
    21841 gttgtaatgg gtgcctctgc aatattcgga atgttagccg gagtttatca ttggttccca
    21901 agaatgtttg gacgtatgat gaataagacg ttaggttatg tgcatttctg gataactcta
    21961 attgctgttt atggggtgtt tttcccaatg cactttattg gtttgggtgg tgtaccaaga
    22021 agatattatt ccaattctgc ttacgaaggc tttgatgtgt ttttaaatat aaacacctta
    22081 ataacggtat ttgcttttct tgcagcagtt gctcaagtaa ttttcctttt caactttgtt
    22141 tattctatat ttaaaggaaa gaaagcacca caaaatccat ggcatagtaa tacacttgaa
    22201 tggacatcac ctgcaggccc aggtattcat ggaaactgga atggtcctgt acccacagta
    22261 caccgttggc cttatgacta tagcaaaccg ggcgcagatg atgattatat tccacaaaca
    22321 gttccgtatt ctcaaacaaa aaactctaac cttccgggtg aagagtaatg tgaattaata
    22381 gttttttcag atgagctact ttgcaaactg caaagtggct catctgtttt aaattactat
    22441 ttcatagttg ttaccaagag taaatattag gttattaaac atatttaaat tgataatttt
    22501 gcaccaatga aatcttcttt tagattgatt agacaatgca tttttgtttt tttgattgct
    22561 ttaatattca atagcgataa tctgtttgca ttttctgact ctacgaaagt tgttgtcaaa
    22621 aagcctaaaa aagagaagta ctataaacgg tcaagaattg ctgttgaaga tgttaggaat
    22681 tatatggata gtataggaat tttaaattct gatatagtct taaaacaagc attgctcgaa
    22741 acaggacact tcaaagccaa acttctcatg gacaaaaaca atttgtttgc atttcgtttt
    22801 accaaacgct atatgagttt tatcaattgg caagcaagtg ttgactatta taaaaaatgg
    22861 caggataaat tttataccaa ccagtcagaa aattactatg attttctgaa aagaataagg
    22921 tatgcgcgtt ttccacatta cattaaagtt ttaaaacagg ttaaggcgcg tactgctcaa
    22981 taaagccaat cataagctgt gaaaaattcc cgaaataacg agcctattaa aattctgttt
    23041 tttcttacag ttattttatc tgtaatctcg ataatgccaa aatatttgac tgggagcaag
    23101 aaaattaatc tctatagcga tattattcat aaggaaaata aaaaggaaaa accgcaaagt
    23161 ttggcaattg taaacgattc tgtatttttt aaaaatgata ctgttaagca ggtattaaca
    23221 gacacagcaa aatctactgc gactaagata aattataagt caggtgaact tgatttaaca
    23281 aggttctttg aagcattgca caatcatgaa aatgaaaatg gaaaaatcag gattgcatgg
    23341 tttggtgatt ctatgataga aggtgatttg gtgacacagg acttcagaaa actgatgcag
    23401 aaaatttatg gtggcagcgg tgttggcttt gttcctgtta cttcacctgt agcacaattc
    23461 aggcaaacaa taagacaaac tttttctgat gactgggaag tttatagttt tatgaaaaaa
    23521 ccaaaacaga attataaact gggaataggg ggatacagtt ttgtttcatc aaagggtagt
    23581 tgggttgatt ataaagcaag cagattctac ccggtatatt cagaggtaaa aatgatttgt
    23641 cgccagtgtc cacctaaaag ttttattttg aacgaagaca cttctttagt tttccctgtt
    23701 gatagtattg gtagttttaa agaggttgaa ttaataagga acaatcctat aaaggaagtt
    23761 aaattaacct ttaaaaaacc aaattgtgaa gtacatggac tttatttcga taaaggaaac
    23821 ggtgtttatg tagataattt ttcgtttcgt ggaaattccg gaattcctct ttccaatatt
    23881 ccttttggtg aattaaagtc tcttggcgca aaagaaaatt acaagttgat aattctaagc
    23941 tatggtttaa atgtagttgc tcatgatgtg aaaaactata gttggtatgc ttcatcattt
    24001 cagaaaacat taaactacat taaagaagca tttcctgatg catcaatctt agtcatgagt
    24061 gtaggtgata aaagttatcg tgcagaagat ggctacgaaa cagagccaga tattcccatt
    24121 tttgttgaat tgcaaaaacg aattgcacaa agaagtgatg tagcctttta taatttatat
    24181 gaagcaatgg gaggttataa cagtatggta agttgggcag agggtaatcc gcaattagca
    24241 aatttagact atacacatcc caattttgca ggtggaagaa aaattgcagg aatgttgtat
    24301 gatgatatta ttaaaagcta tgatagatat agtaaaacgg tggaaactat ggaagtgaaa
    24361 agagatacaa tgattaagcc tcaggcaaaa caatgaaaag aataatttta attttttctc
    24421 tgttaatata ttgtacaaaa tgtcatgcac agagtgattc cgatactaca atgcctgtgt
    24481 tgcaatatga gaaatttgtt gaatataaaa tgtctttatt gaaatttctt aaacccgatg
    24541 caattgatat tgaacagaaa gaagcattga aaaattttta tagtaaattg ttgcagagta
    24601 gagctgacgg gaaaaaagtt agtatagtgc acattggcga ttcgcattta caggccggat
    24661 atttttcagg tatggttcgc agaggactac aacaacgcta tggtaatgcc ggtaggggaa
    24721 ttgtatttcc atacaaactt gcgcgttcta acggcccgca tgactatgtt tctttttctc
    24781 agaatagctg gaaaggaaat agaaatgtaa ttacacaaga ttctttatca accggcatca
    24841 ccggattttc agtaacaacc aacgacagta gcccatctgt aggaattagc ttacgaaata
    24901 atgaaggtat agattataga tttaacagag ttcaagtatt ttataaacct gacagtactt
    24961 ttatgactgc aagtgatagt ttagcaataa ttgaaacagt aaacccaata aaacttgcaa
    25021 caggaattaa tgagttcaga ttttctgatt atttatcgaa ttgtaaaatt aacatcagtt
    25081 ctgatactac ttatacatta ttcggtttaa atcttttgaa tgatagctct ggtgttgttt
    25141 atcataatat tggtgtaaat ggtgcagagt ataaaagttt tcttgggcaa ccaatgtttt
    25201 ttgaacaagt aaaagaactg aatgctgacc tgtatattct ttcatttggc acaaacgagg
    25261 ctttcgcaaa gtattttgat ggaacagtat ttattcagac cgttgattcc tttataacta
    25321 aactcaggaa tgtttcacca acagcgtcaa tattgattac ttcaccaacg gaatcatgta
    25381 aacgtataag acgtggtgtg tatgtaccta atccaaatgt aattgctatt aaaaatctcc
    25441 ttcagaaata ttgcatggat aataaaatag ctttttttga tttatatgag tgctcaggcg
    25501 gcaagggaag tatgcttcgt tttgcaaaaa ataaaatgag tgatgccagg cgaattcacc
    25561 tgaaccgttc aggatatgaa atgcagggat taatgttgct tcaggcgctt cttaaaccat
    25621 tatctgaaca atgacaatag attgggaaaa gcttggacat cagtttttgt atgataaagc
    25681 aaatccattg cttttcaaca gttcgttttt tcttttttat ttgttgttgt ttttgttcgt
    25741 atttagactt ctgcaacgaa caggaacttt acgaattgtt gcagtaacac ttttctcact
    25801 ttatttcttt tataaagcct caggagttta tgtgcttttg gttcttgcgg ctgccgtaat
    25861 tgattttaac ctcagtaatt ttatttacaa tgcaacatct gattttcgga gaaaagcatt
    25921 actcgttttc agcgttgtca tcaatcttgg actacttgct tatttcaaat acaccaacct
    25981 gttaattgac ttgtataatg ccatactaac cggaaatgtt cagccaatag atgtctatct
    26041 gccaataggt atatcatttt acactttcga aaatctgagt tatactattg atgtttacag
    26101 aagacagttt gtgccggtaa aaaaatttat tgactacctg ttttttcttt catttttccc
    26161 aaaactaatg atgggaccga tagtgcgtgc agcagatttc atacctcaga taagaaaaga
    26221 gattgcactc actgcacatg atgtatctaa aggcttgttt ttaattctga ccggattgtt
    26281 taagaaggtt gttatttcag actttatcta tctcaattta gttcagtatg tgtttgatga
    26341 tcctttgcgg cacacaggta tggaatgtct ttttgcagtg tatggctatg cattggtaat
    26401 ttattgtgat ttttccggct atagtgatat ggcaataggt attgcaaagt ggataggtat
    26461 agatatcaat attaactttc ttgcacccta tcagtcggcc tccataacag agttctggcg
    26521 cagatggcac atctcacttt catcgtggtt gcgcgattat ctttatattc cgttaggtgg
    26581 taatcgtaaa ggagaagtaa gaacctacat taatttattt atcaccatgt tgcttggtgg
    26641 actatggcat ggtgccagct tgaattttat tttttggggt gcattacatg gtgtggcatt
    26701 agcttttcat aagttatgga aggcagcata tccatcacat ttttccggac gtttaacacg
    26761 cattgcaggc atcattataa catttcattt tgtttgtctt tgctggatat tttttaagtg
    26821 cacaaccttt actgacagtt ttaacatgat tcatcagatt gtgtttaact tctatgctga
    26881 aggttggaca ttattcagtg ccaattatag tgaagtgttg ttagtgatgt tactaggatt
    26941 tgtattgcac tttttgccaa tccgatggaa tgaaattatt atcaattata ctgaaaagtg
    27001 gcattggtta ctaaaggcat ctgtagcaac ggcagttgta atttttgtaa tcgttcttaa
    27061 acaagccaat cctgtcatgc cggtttatct tcaattttga taaaaaattc tcgatataaa
    27121 tctatatatt gaaatctttt ttaattttaa atttggtttt tcaaagtgga ggaaaaatga
    27181 aaagactttt tattgtaatc agtgtatttt ttattgcggc atgtaaagac agtggaaagc
    27241 agtcaataaa agacacatct gtattgttaa aggaaattac tgatggctat tatcaggagc
    27301 gaatgcgcta tttccctctt gaggccacag caaatgccga taaccgttat aatgatttac
    27361 taccaattga tatcagtgat gcttatattg acacacttcg tcaattctat caccgttact
    27421 ccgaaaagct gcttactatt aacaagcctg aattgacagg tcaggattta ataagttatg
    27481 aggtgttaca atatacttta aacatagagg aagaaggcct gaaatttcct tcaaacctaa
    27541 tgcctgtaaa tcagttttgg ggtatgcatc tcacattttc tcaattgggt tcaggcacaa
    27601 gctctcagcc atttaagact gtaaaagact atgataattt cctgcaacgt atttctgcct
    27661 ttgtagctta tcaggatact tccataaata atatgaaaag aggaatgtta caagggtttg
    27721 ttcctgcacg tatcctcatt gaaagaacaa ttccacagta taaatcactc attgcaaaca
    27781 aagttgaaga gagtgttttt tacgggccaa ttaaaaatat gcccgatagt ttttctgatg
    27841 cagataaaaa acgattaacg gatgcttact cgaatgcaat ccttaatcaa ttaaatccaa
    27901 gctttgaaaa gatggcagtt tttttagaga aagaatattt gcctgcttgt cttcctgttg
    27961 ccggtatttc taatattccg ggaggaaaag aatattataa ctggcttgcg aataaatgga
    28021 caacaacaac aatgagtcct gatgagattt ataatttagg ccttaaagaa gtcgaacgtt
    28081 tacattcaga gatggataaa gtaaaagcag agacaggttt taaaggatca cttaaagaat
    28141 tttttcattt cattcatact gacccacaat tttttccatt taagaaagat tctgatgtaa
    28201 ttgcagccta tcttgcaata gaagacagaa tgaaaccaca gttgaaaaaa ctttttaatc
    28261 atgtgcctaa ggcagctttt gaggttaggc agacagaagc ttatcgtgca gcatctgcca
    28321 gtgcagagta taacacagca gcacctgatg gatcaagacc cggggttttt tatgtaccta
    28381 ttttagatgc aacaaaatat aatgttgtag gtatggaaga tttgtttctg cacgaagcca
    28441 ttccaggtca tcattatcag atttcattac agcaggaaaa tacatcacta cctgagttca
    28501 ggcgctttgg ttggtttgga gcctatggcg aagggtgggc tttatataca gaatcattag
    28561 ggaaggaatt aggattatat acagatccct atcagtattt tggttgcctg agcgaggaaa
    28621 tgcacagagc cattcgtttg gttgtagatg tgggtattca cctgaaagga tggacgcgcg
    28681 aaaaggcaat tcaatattca ttggataatg aaatggaaag cgaggctgat attactgcag
    28741 aaattgaacg ctacatggcc ataccgggtc aggcattgtc gtataaaata ggtcagttaa
    28801 aaattattga attacgtcaa aaagctgaag agcagttaaa agataaattc agtattcaga
    28861 aatttcatga tcagctactt tcctatggtt gtcttccatt aagtgtactc gaaaaatcca
    28921 ttaaactatg gattaaagaa cagaaaaagc aaggctgaaa ttacctttca caagtataag
    28981 ttttcgtaca aatagcttat gtttgcagca taacatttta agtttttctg ctgtatgaat
    29041 atgaagcaac taatcgagaa ttttccttcg caaatgcgcg aggctatgac tattggcgaa
    29101 acatttaaag ctacaagctg gccacatgaa ataaaaaatg tattggtgac cggacttggt
    29161 ggatctggaa ttggaggaac cattgtttca caattgtgtg agaaagagat gccgatacct
    29221 tttatggtga acaaagatta ttttatgcct gcatttgtaa acaaacattc gctggtagtt
    29281 gtaagttcct attcaggaaa tacagaagag acacttcatg ccatggatat ggcaataaaa
    29341 caaggtgcaa aagttgtatg tgtcagctca ggaggcaaaa cagttgaatt agcaaaacaa
    29401 catggattgg atcatatatt aattccgggt ggcatgccac cacgttcctg ctatggatat
    29461 tcatcaacac aacttttcta tatactacat gcttttaaac ttataggtga tggttttaaa
    29521 gcctcattga agaatgctgt tgatttgtta gatgcagaat ctgctaatat taaaaatgca
    29581 tcacataagc ttgcatcaga gctgattggg aaaattccag tcatttattc agcttccaat
    29641 aatgaaggtg ttgttgtgcg tttcagacaa caagtgcagg aaaatggaaa aatgttgtgc
    29701 tggcatcatg tttttccgga gatgacacac aatgagcttg taggatggac acaaccacat
    29761 aatgatattg ctgtagtgtt gttcagagat aaagatgatt ttttcagaac agcaaagcga
    29821 atggatattt gtaaagagat atttggtcgc tacacaccac acatttacga agtatggagt
    29881 aaaggtggtt caccaataga aaaggcaatt tatttgattc actttggtga ctggacaagc
    29941 tggtatctta gcgaaatcag aaatatagac tgtacagaag tgaaagttat tgactacctt
    30001 aaaagtgaaa tggccaaact gaattaatta ttctgtttga atctgtcatt catgaaaaaa
    30061 gaaaatttat ttatagcagt agagggaata gatggtagtg gtaaaagtac acaggtacaa
    30121 ttgctgaaag ccaaccttga agaagccggt tataaagttt atgcaacaaa tgaacccacg
    30181 aattttgaag caggaaaact cattcgttca atttttaatc atagagtaaa tgccgatcag
    30241 cataccattg cagcattgtt tgttgcagac agattgcagc atttgcttga taaggaaaat
    30301 ggaattctgg caatgcttga aaaaggctat acggtaatca cagatcgcta ttatttttcg
    30361 tcctatgcct atcatagtgc ctatgtagaa atggattggg taatgtctat caatgctaaa
    30421 tgcgcagcgt tgttaaaacc tgacctgaat atttttgtag atgtagatcc tgaaactgca
    30481 tttaaacgca ttattgctac acgcacctca acagaattgt acgaaaccaa agaaaacctt
    30541 cgtaaagtct atgataatta tcttaaagca ttcaaattac taaagaatac agaaagcatt
    30601 gtttttgtta atggcaaaaa tgatgttcag caagtagcag ctgatatttt caaaaccgta
    30661 aaaaatttat tgcataccta aaaaaattga ttgttttttt agtttagttt tttatcattg
    30721 ctgaaatttt aaatcgtttc aaaatgaata agaagcatcc aaaggcattg ccctacctgt
    30781 tcctgtctga aatgtgggag cgattcggtt tctatctcat gcttggtatt tttttccttt
    30841 acatgacaga cacacaacgt ggtggcatgg caatggacag aaaagaagct tcagatattt
    30901 tcggaacatt cattgcatta gtttacttga ctccgtttgt tggtggatta attgccgata
    30961 gagttttagg atatcgtgtt tcaattacac ttggtggcat tcttatgggt ataggatatt
    31021 gtggattggc aatacccggc atgactgcat tttatgtttc actattactc atcatcattg
    31081 gtaatggatt ttttaaacca aatatttcga ccattcttgg taatgtgtat aacgatccgg
    31141 agtataagcc actcaaagat tcgggataca atatttttta catggggata aacatcggag
    31201 catttatctg taattttttc ggctcttatt tgcgaaacaa cttcagttgg ggtgctgcct
    31261 ttgccgctgc agggattgga atgtttgtag gtgtcattat tttttggatt ggcaataagc
    31321 actatgcaca tgccgatgtt cgcaagcctg taaagcccga agatatgcct gtatcaaaaa
    31381 tattaggtat aacattatta cctgcattaa ttgcagggta tattggctgg ataattcctg
    31441 aaaatatttt cggtagcgat tcaaccgatg catttatttt aggtgcccta cctgttgccg
    31501 gatattatgt aaatatactt ttgcgttcag ataaagagga cagacggcca ctagctgcat
    31561 tgctggcagt atttgctgtt gcaatagttt tttgggctgt gtttaaacaa aatggaacag
    31621 cactaactac atgggctgaa agttatacca atcgtgaagt accacagagt gtagaacctg
    31681 ttgctaaagt gcttggagta acacaaacag taaccttctc aaaagactct gtaccattaa
    31741 ccgatagtca gtttcgtgtg gcacgtgacg aaaataaaaa agcaataaaa acatgggatt
    31801 acccacttta ttataagaat gttcctgcag ataaattacc taaagaaggt gaatcgctaa
    31861 cattaatttc aacagaatta ggtcaatcaa ttaatccatt ttgggttgta atattaacac
    31921 ctgtgattgt tgctttctgg gcatttctca aaagaagaaa cagcgagcct tctacaacat
    31981 ggaaaatatt ttatggcttt ctcattacat cattgtcaac attggtaatg gtcgcagcta
    32041 cttatgcatg tcataatggt ttagagaaaa gttctatgtg gtggctcatt gcaagctatg
    32101 gagtaataac tgttggtgag ctttgtttaa gcccaatggc attgtcattg gtatcaaaac
    32161 taagtccacc acgacttaca gcactgatga tgggcggatg gtttttatcc acatcaatag
    32221 gaaataaatt gagtggcgtg ctggcaagta tgtgggatga atatgaacat aaagcaaact
    32281 ttttcttagt taatttcaca ttagtaatga taactacaat aattatttta ctgatgttaa
    32341 aatggcttaa taaaattgtg aaagagcatg gagcgtaata gtatattatt gtttttattc
    32401 tgaaagttta tttgcatttt attcttgaat tagtacggca gataaaaatg tttaaactct
    32461 ttttagtaga gaggtttttt aatttgaaaa tttcaacccc tcacatgata atatcagacc
    32521 ttcacccaaa acatatatta cttgcacaga ttttgaagta ttataggtat aattcaaaat
    32581 ccgagtatta tgagattact atctactgct gttattttat taggatgcct ctgtgcacaa
    32641 gctcaaatta gtatcacaaa taatgaaatg ccttctgccg gtgatacagc acgctttaca
    32701 aaagctgttg taaatccatt gataaacttt agtgcaacag gcaccaatca cacttggagt
    32761 tttggtaacc tccgcaacaa tggacaagat ttaaaatctt atcaaaatgt ttcatctaca
    32821 ggatttattt attctttgtt tttttctaat ctgccattta atccaaacag agcaaacgta
    32881 acagaacctg gacaagcatt accttcaaac cctttgttga caattaccga tccatataat
    32941 ttctattatc gttcaagcag tgtatataaa caagtaggct tcggtgcaga gattgcgaat
    33001 attcctatgc ctgttgcctt cagtcagcat gatattattt acaacctgcc attaaattat
    33061 aacaattcag atacctctca ttcagcatgg aatatcggtc ttcccggctt aggttattat
    33121 ggacttacac agacaagaat taatcacgtt gatggttggg gaacgcttac aacaccacat
    33181 ggaacattca acaccttacg tgtaaaaaca caattgtata taaaagatac tataagtgtt
    33241 gacacattaa atctgaattt tgctttaaac agaccaaaga caacacaata taaatggtta
    33301 gccaataatg aaattattcc tgtgctacag attacaacca cagaaatttt agggttagaa
    33361 gtagtgacag aaattatata tcgtgatgat tacaatacgg tatcaccagg aagtttaaat
    33421 gcagcatact gtgccggcag taatgtatca attccatata cagctacagg tagtttcaat
    33481 ggttccggat ttttacaaac tgctaatagt tttacagcac agttaagtga tgccactggc
    33541 agctttgcaa atgctgtaaa tattggaagt gttacttcaa gagtttctgg gaatattaat
    33601 gcagtaattc ctgcaaatac tcctgtagga aatggttaca gaatcagaat tatatcttca
    33661 agtcctgcat taacaggagg agataatggg tttgatttaa gaattgagaa caatgcagtt
    33721 gcttcaattg ttaatcaggg tttatcggca ttctgcagtg gcaactctgc tttattacag
    33781 tcagttaatg ttgattccgg atatacttat cagtggcaac taaatggcac agacatttcc
    33841 ggagctacta cagataacta tttggcatca gcaacaggca actatacatt aaacattacc
    33901 aactcatgtg gtactattac atctaatgct gctgcaatta atgttttgcc aatgccaacg
    33961 gttcaggtta ctgcatcagg accattaaca ttgtgtctag gtaccacagt tacactttct
    34021 actttatttg attctacata ttcctatcag tggcaattga atggtgttga tatttccggt
    34081 gcaacaacag aaaattacac tgctgcaagt ggaggagatt acacagttgt agtgagtaat
    34141 agttgtggta atgtaacatc gtctgttcaa acagtttcag ttcaatcagc tccagttgca
    34201 ggaagtctca ctgcagcttc gtcaacaata tgtcatggtg attctgtaca aattaactat
    34261 tctttgcagt ctgcaggcat tacctggttg cataatggaa atattctttc aggcattact
    34321 accaacagtc attttgcaaa ttctgccggt gtgtattctg tcgttgaaag tgacttatgt
    34381 ggtagtgata cttccgttgc tgtaatactg acagcggaca gtttaccaac tgctgtaata
    34441 actgcttccg gggcattgtc attctgtgca ggaggagatg ttgtgcttac agcagcaacc
    34501 ggtaataatt acgcatggca atggtatatg aatggaacgc ctttggcaac tacgcagtta
    34561 ataacagcaa cacagagtgg aaattatgaa gtggaaatta caaatgcttg tggctctgtc
    34621 atgtcaactc ttgttgcggt gactgcattc ccattacctg tagtaccgac aattacactt
    34681 tctaacgatt cattattagc gacaacgact gtaggtactt atcaatggtt ttacaacggc
    34741 aatgccatta ccggtgctac aaatgacttt tatgttccgt cacaaaatgg aaactacagc
    34801 ttaatggtta cagatgctaa cggttgttca tccatgtcag cagtgtataa tatgacaaca
    34861 gttggattgc aaactatatt taaacaagca cataatgtgt ttccaaatcc gtttaacgac
    34921 tatctgacaa ttgaattacc acagacagaa aatgcaatta ttttgactgt tgaaggaaag
    34981 caattgtgga gcggtacgtt gcatcagggt aagaatgtat tgaatatgca gcaatttgtt
    35041 agcggcatgt atattctgca actgaacaat gaaaatggaa tcacaaacta caaattagtt
    35101 aagcgataaa cattgtgtta tctgaacaaa aggcaatttg ctttttgttc agatttaact
    35161 gttatgattt gcggtattta actcctctga ccgcatttca acagtagtct gttctgtttc
    35221 aggagttgtt tcaatgtgct taaaaattgt tcggttaaaa agaataatta ataaaacacc
    35281 tgcagtaatt gctgaatcgg caatattaaa aacaggtctg aaaaaaataa attcttctcc
    35341 gccccaaagt ggaaaccatt gcggataatg tgtctgaata atcgggaaat aaaacatatc
    35401 cactacttta ccatgtagcc atccggcata gcctccggca tcgggcataa aggttgcaac
    35461 accaaattcg ctggaggaaa aaaggcggcc gtaaaaaata ctgtcaataa tattaccaac
    35521 tgcaccggca aaaattaatg ccatagctgt ggtgtaaatg tgatgcgctt ttgttttatg
    35581 cagatgccat acataccagg ctaatgcaaa catgaccccg atacggaaaa gtgaaagaaa
    35641 tttttttccg aacaattcca tgccaaaggc cattccatta ttttcagtaa aatgtatgat
    35701 aaaccaattg cctgcaatat ggtattcctg accgagatac atattggttt taatccagaa
    35761 tttcaaacac tgatctaaaa tcagaattac ggaaacaaga gcaatatatt ttttcaattg
    35821 tatggattga tataaaatga ggattgccaa aaatgttgac aatcctcgta ttgtgtcata
    35881 aataaatatt aatcgcgata ctgttgtacc ttggcttcaa tgctttgtgt agtgtgtggc
    35941 acagcacgaa gtctttcttt tggaatcaac ttgccggtta cacgacaaag gccatacgtt
    36001 ttgttttcga tacgtaccaa tgcattttca agttgctcaa taaacttacg ttgacgcgca
    36061 gcaagttgtc cggcttcttc ttttgctaaa gtgtcgctgc catcttcaag catcttaaat
    36121 gatgaagcag tgtcgtcagt accatgttca ttgctgttgt taagaaatgc ctgcaggttg
    36181 tttaactcaa cttttgcatc agtcaatttt tgaataatca actcgcgaaa ctcgtttaat
    36241 tctgcatcac tgtagcgtgt tttatccggg ctgttttcca actttgtagg tctgttgtca
    36301 agcattgcaa ccggtttttt aagaatagaa gcaacaggtg catctggaag aacagggtct
    36361 tcacgtaatg gcgtagtacg cggaggagca gccggtttaa caattggagg gttgctgcgt
    36421 ctgcgtgaac tttctcttgg cttctctacg tgtttaatca tagatgttgc cgatttgctt
    36481 ttatcctctt tttgtgattt tgcagttaca ccaggcttta taattttaat tggttttggc
    36541 ttaacaacca ttgcaggttt aacaaccttt gctttagcaa caggttttgc ctttgctact
    36601 ttttttgcaa ctgctttgct tttaattact ttgctttttg caacagcctt cttagttaca
    36661 gtctttgatt tagcaggttt accttttgct actgcttttt tagcaactac tttttttgct
    36721 acaggcttac ttttaacagc agcctttttt accactttaa caactttttt aacagctgtt
    36781 tttttatttt ttggtgtagc tttttttgct gcagcctttt ttggtgcagc tttctttgct
    36841 acaggctttt tcttcgcttt acttgccatt tttaaatcct ttctatttca attaatgttt
    36901 taactccttc ttcaacctct atttcatcgg ctaccggtgt agctaatgtt tctacccatg
    36961 taaatgagga ggctaaaatt tccgcgcaaa tatagtctaa attactttcg attgcagtga
    37021 ttatagatgg ctgattttta agtttaacca gtattttatc cgttacctca aatcccttgt
    37081 ctttacgcag gttttgcact ctgttaatca gttcacgggc aatgccttcg tcacgaagtt
    37141 ggggcgatat actgatgtct aatgcaaccg ttaaaccccc attactattg acctgccagc
    37201 cgggaatatc ttctgagctg atttctacat cttcgaccga tattgcaact atattttggt
    37261 tgattctgag gtcaaatgtg cctttacttt ctaagttacg tatgtcatcc tgagtcattt
    37321 gtgctatggc agcagcagca tctttcatga gtgcccccag cttcttgccc agcactttaa
    37381 agttggcttt aattttcttt accaacaatc ctgaggcatc ggtaacaaac tccatttctt
    37441 tcacatttac ctcagtaagt atcagttttt ccacctgttc aatagccatt tgttgctttg
    37501 ggctgtccac aggaatgagt attttttgca agggctgtcg cactctgatg tttactttct
    37561 tacgtaatgc cagtaccata cttgaaatac gttgtgcaaa atccattctt tcctctaagg
    37621 cagtatcaat cattttagtt tctgccttgg tcagataggt aaggtgtaca gaggtatatt
    37681 gcaatggtgt tttagctgca atggcattgc tacgcatgct atcggtaaga tttctgtaaa
    37741 gccagtcgga gaagaaaggc gctaccgggc tcatcaactg tgctgtaacc attaaacatt
    37801 caaacagcgt ttcataagca gattgtttgt cggtattcat ttcgctgcgc caaaaacgtc
    37861 ttcgtgaaag acgtacatac cagttgctga gatgctcatc aacaaattct tcgatctgac
    37921 gggctgcaaa tgtgggctca taatcgttca gactatccgt gacacgctgc actaaattgt
    37981 tgagctttga taatatccag cgatccaatt cagatctgtc ctgcacgggt actttatttt
    38041 tttcatcagg aacaaaacca tcaatattgg catatagtgc aaaaaacgaa taagtattgt
    38101 atagtgttcc gaaaaatttt cgttgcactt cggcaatgcc atctacgtta aatttaagat
    38161 tgtcccaggg ctgtgcattg cttatcatgt accaacgtgt agcatcgggg ccatacttgc
    38221 caatagtctc aaacggatca acagcattgc ctaagcgttt actcatttta tttccgtttt
    38281 tatcaagcac caatccgttt gacacaacat ttttaaaagc tactgaatca aacagcatta
    38341 ctgcaatggc atgtaaggta aaaaaccaac cacgagtttg gtcaacgccc tctgcaataa
    38401 agtctgccgg atagcctgtg ttaaaccctt cgttgaaagg cattggtagg cctttactta
    38461 atttttcctg atctaattgc cattgtgcat agggcatagc accactgtca aaccacacat
    38521 caatcaaatc agtttcacga tacatttttt tacctgtatc tgaaatcaga ataatgttgt
    38581 ccacataagg tctgtgaaga tcaaagtcag atttagaagc atctacatgt tccagatgat
    38641 gtgcatccat aaaacctgct gctacagctt tttcaatttc tgtacgaagc tcaataatag
    38701 agcctataca tttttcttct ttttcatctt cacttcgcca tataggaaga ggaattcccc
    38761 aataacgtga gcgcgacaga ttccagtcaa ctaagttttc aagccagttg ccaaaacgac
    38821 ctatacctgt tgactccggt ttccagttga ttgttttgtt gagggcaatc attttgtctt
    38881 tgcaggctgt tgtacggata aaccaactgt ctaaaggata gtataaaaca ggcttgtctg
    38941 tccgccagca atgcggataa ttatgctcgt acttttctac tttaaatgct ttgttttcct
    39001 gctttaattt gatggcaatg cgttcatcaa ctgacaaata tttatctctt ccttgtttct
    39061 gacgctcggc ttctttttca ttcgtagtga gatattcttc ttttacatac tctcctgcaa
    39121 agtcagtaac ttctttcaca aaacggcctc tcttatcaac taatggtgaa ggatttccac
    39181 cttcgtcagt aactaaaatt gcactgatgc cttgttgttt tgcagtacga aaatcgtctg
    39241 caccaaaggt tggcgctatg tgaacaatgc cagttccatc ttctgtgcta acaaaatctc
    39301 ctgcaacagc aacaaaagct tttccgtcag gttttacata gggtaacaat tgctcataag
    39361 caattcctgt cagtgtggaa cctttacatt ctgcaattac tttaaaagga atttgtttgt
    39421 caccgggttt aaaatcttca agactaagtg ctgcattttt ttcactaaaa tattttgcga
    39481 acaaatcttt ggcaagcaca acaacaatag gttcgttggt gtattgatta aacgtcttca
    39541 cccacacata accgattttt tcacctactg ccaatgcgct gtttgaaggt aatgtccagg
    39601 gtgttgtagt ccatgcaaga aaataaaagt cacaatttat attctgcaaa tctttttgta
    39661 aagactgtgg cagtgtttct ttttttattc tgaactgagc caccacagtg gtatctttca
    39721 ctattttata agtgcccggt tggttcaatt catgcgagct gagacctgtt cctgctgccg
    39781 gagaataagg ctgaatggtg tagcctttat acaacaaatc ttttttataa agctctttca
    39841 gcaagtacca gcaagtttca atgtagccat tttcaaatgt gatgtaaggg tttttcagat
    39901 ctacccaata gcccatttta cgtgtcaggt catcccacac ttctttatac ttcatcacct
    39961 ctttgcggca ggctttgttg taatcttcaa cagatatttt tttacctata tcttctttgg
    40021 taatacctaa agttttttct acactaagtt caataggcag tccatgagta tcccatccac
    40081 ctttgcgctt tacctgatag ccctgcaacg ttttaaaacg gcaaaaaata tctttaatgg
    40141 cacgtgccat aacgtggtga atgccgggca agccgttggc acttggcggc ccttcataaa
    40201 aattataagc cttagcgccc tggcgtgagg tgatgctggc atcaaaagtt tgctcgttat
    40261 cccatttcaa tagaacgtca ttggcaattg ctgccaaatc caactgcttg aattcaggat
    40321 atggtttatg actcattaca ctgttttttc aaagagtgca aaggtaagaa gttctgctat
    40381 agtggaggat gagggaaagt tgacattttt tggaaagaaa atgacctgaa atgccttttt
    40441 taaagcgatt ttaaaatatc tgtttttatt ttcatgatga catttgttat tcgtaacttt
    40501 gaagattaat agtaaaaatc attacaatga aatcattatt cctttcagta cttttttatg
    40561 tactatcaaa ttttactatg gcgcaggtag cctctaaatt agcttcaaaa cccgatgcag
    40621 ctaaagagtt gattacagtt gaaacttctt gcggagaatg taattttaaa ctgcccggag
    40681 ataattgcga tttagcagtg cgcattaatg gcactgcata ttatgttgat ggtgtttcaa
    40741 tttctgaatt cggtcatccg catgatgaca atggattttg tgttgctgta cgcaaagccg
    40801 aagttcaggg agaagtggta aacaaccgtt ttaaagcaac ttattttaaa ctattgccgg
    40861 ttgccaatgc atcagaaaat tccaataaac aagatggagg taagtagctt tttagttctt
    40921 attatctacc actctgttta aacccctgat taatgctatc aatataatcc aacacctcct
    40981 gacggccact ttttgtatcg gcagaagtga caaaatatgg aggtagtgtt gcccaggttt
    41041 tatataactg ttcgcaaaac tgctttacag aaaaatcgtt tttggcacgt gtttgtttat
    41101 ctgctttggt aaaaaccaaa ctaaatggaa tagacttttc accaagccag tttataaagt
    41161 caagatcttt ttgttgtggc gaaattctga tgtcaattaa cacaaaaaca gacatcaggt
    41221 ttttacgata aatcatgtaa ttgcgaatca taccatccca tttttcgcgc tctgtacgag
    41281 cagtttttgc aaatccatag ccgggtaagt caaccaaaaa ccattgccca ttgatgataa
    41341 aatgattaat cagttgtgtt ttaccgggag tagaagatgt ttttgccaga cctttcttat
    41401 tacaaagcat gttgatgagt gatgatttgc ccacattact tctgccaata aatgcatact
    41461 ccggtttatt gggttccggg cactttttta catctgtatt gctttgaaca aattctgctg
    41521 aacttatttc cataatttta atcaatgcaa aagtaaattt taatcttcaa ccggaatcat
    41581 gtagtttcgt atccgctttt tacaatgaga aattcttttt atatcttgat tttcgctctt
    41641 gtcgttctga cctcatgtca taacgataaa agttccgata atccaccatg ccctgcaatg
    41701 gaaatgcaca tcagtggcga tactacagta gttgccggtg acagcattgg cttaatggca
    41761 actttgttta ccggggccac ctattcatgg acaggcccaa atggttttat tgccaacacc
    41821 tataatcctg taatttccga atcatcatca ttaagcgagg ggtattacta tgcaagtgca
    41881 gacattaatg gactttgcaa gagtaatgtt gactcttttt atgttactgt aatttgcgat
    41941 cgtccggaag atgtgcttgc caattacatt acaactattc aaaccaacga caccattcat
    42001 ctgtttgcac actctattac cgattctgtt gaatatatat ggacagggcc ggcaggattt
    42061 acttctacac agcccaatcc aatcatcacc aatgccattc cgggcaatga aggtgtttat
    42121 actgtttatg cgcacaaaga tcattgctat agcaagcccg acagtgttgt ggtaagtttt
    42181 aatcagtgca acccatcgtc caacacattt gcaaacacat tagatagtaa ttattttctg
    42241 tattcctatg gcagtgctaa tctttaccct aacacaagaa gtattacagg aacggggctt
    42301 aacggaactt cgcaattgcg tgctacgttt tatggcaaca atccaccaac aggaaactat
    42361 attataaatc cggcacattg tcctgcaagt ggaagtggag cacttgcacc cggtgaagtt
    42421 tgtgtggaat tcagtaatgc atcaggaact tatcaggcag taagcggtac ggtaaaatta
    42481 aaagcattaa acacattcga tttctgcgat gcaccattcc gattacagtt tacaactact
    42541 acacttttta acggcagcgg caaggttcag ttttaatgtg ccggatgata ggtgtttaca
    42601 tgtctggctt ttttgctttc gtaaatatca tcaatagaaa ttaaaatacc ggtttcttcc
    42661 acatcgccaa attcatgatt taaagcagtg ccaaaagttt tcattgttgc tgaaagattc
    42721 atgtagctgt taaacagtgg aggaatattt tctccaagtt cgcgcacttt tttattcaaa
    42781 ttatggtgac cttctttgta aggaagattt ttcaggcttg ctacaaaatc atgtacatca
    42841 ttttctgccg gtaatgggtg gtgtggataa accattttat ctttatcagg aaaatagtgt
    42901 tgcataaaag caagaatcaa atctctggca atgcggttaa aacgcaaata catggttacc
    42961 tttccaaaaa aatatttcat gtctggatta tcaatggcaa tagcacctaa cccatcccat
    43021 aaattgtcga gagaaaaaag gcctttacga ttctctgccg aaggttgata ttgtggttgt
    43081 acaaacgagc ggcctaattc aatggtatgc ggaaaatagt ctttgataaa tttttcggtg
    43141 aaatgaaaca gttctgttgt ggctaacttg tattgactgt gcgggtcagt ggcagcatcg
    43201 gcacagcgta tgaaacgata gccaccaaca atttctttat cggcaggatt ccaaactata
    43261 agttgcttgt agcaattttc aataagatca aactcatcta aatcaagtgc ttttcctgtg
    43321 ccaccaccgg cagctctgaa tgtaagctca cgcacacggc caatttcttg cagtacatta
    43381 ggcgagtcat gagctgtaac cacatacaac ttgttctgcc cattgttggc atcgcggata
    43441 aaacggccat tgtttaactc ctgctccagc aggtgtcttt cgaccgggtc aatgatttgc
    43501 atacttcacg aaaatagaaa aagtattttg attgtatgaa tgcagcaaaa aagatttgaa
    43561 acagcttttt tgtatatctc tgaaaaggat aatgtcatta aaccaaattt gatagctttg
    43621 cacgctattt gaatctttat cgtaataaaa tgaaaaacgg acttttaatt tactgtgtac
    43681 tgcttactct ggcagtagct tatttatttt acaaagtaaa cagctgtaca agcaattgtg
    43741 ctcctgcagg cgttgccact gccatgaatg atggcaatat tgtttttgta aatgttgata
    43801 cattactcga gcactacgac tattatatta gtcttcgtga gtcgcttaaa aagaaacaag
    43861 acagcattga tgtagttctg aaaaacaaag gcaaagaatt ggaaggcgaa atagtaaatt
    43921 accagaaacg tggtgccagc atgacagatc agcagcgcgg tgccgaagaa gaaagattag
    43981 gacacaaaca acaacagttg gttgaatata aaaaccattt acttgatatg ttgactgaag
    44041 aagaagctgt catgcaggat agtttgcaca aacatttagt tggttatctt aaagacatga
    44101 ataagcaaac taattttaag tttgttttcg gctatcagcg cggcaatgga attttattag
    44161 ccaacgattc gctcgatatt acacaaagtg ttatagaagg actgaacaaa aaataatcat
    44221 cttttaaaac ttcgcaccaa aagtcatcat aaaacgattg attttggtat taattgatgc
    44281 tgatggcgca ctgccatctg ctaaggtgta aggtgcataa ttcccgcttg ttttagtata
    44341 ggcataacct aaatcaagaa aaaagtttcc acctttaatt cctaagccac cggtgtagct
    44401 cactgcacta cggttacctt ctttgtaagt tattccacct tcataggggc ttgattgcat
    44461 tacagttccg gccctgaaac tgaaaatttt atatcggtat tcagcaccta tttttaaaat
    44521 ctgaccattt ttgagacttg cttttatatc tctgttttcg tcagaaaaac tgtactcatt
    44581 ggagttgagt cgtgctgtag aataatctaa gtattcatag tcggcactaa tcagtccatg
    44641 atcaagaaat accaagccta cacttgtaat aactttaaaa ggtgtggtca ggttgtaaga
    44701 aaatctgcca tcgggtgatt tgttttccaa atatccttca gaaaaatttg cataaatttt
    44761 tgaagaatac tgatcgttca tctcataata ggttggtgtg tgaatggcca atcctaaccg
    44821 aaccatatct gtaggtttaa aaatgacacc taatttcagg tttacgccat tgccttgcgt
    44881 tgaaagcttt tgtgtgtatt ttgaattttt gaaagtttta ccgctcagac tatctttagt
    44941 tgtgttttgc aaggcatact cctcatatac ttccgttgaa gaatatctga aataaggaat
    45001 accgacagta gcaccaagat aaacctgatt ttcgtagtta gcagcaaaag aaatattaat
    45061 ttcgccttgc gaacccgtgc gtgtgatgag acctttttgt tttaaatcgc cattatagag
    45121 cgcaccctga tactgatcta tcaaactgct gtctgcaaca ggatttgtca gataactgtt
    45181 atatgccaaa cctgcatcat actgatacat atcttcaccg gataaatttg ctttgctgat
    45241 gccattactg atttcagcaa aataatcatt aatagaatta tttttattta ttccttcaaa
    45301 agcaaatctg tcgctgaatg agttgatacg gttgtagccg aatcctaaag atgttcccat
    45361 ccagcctgaa gtttttttct cgcgtggaaa ggccagcaca aagcccagat tgcctacacc
    45421 taaatttact ttattgtctg atgttagtgt acctaaatag gtggcatcgc tgtttccaaa
    45481 ttgcaaaaca ggactgaaag taaactcgct tctgcgataa acggcaatac cggccggatt
    45541 tattgaaagg ctagaaaagt cggcacctaa agcaccaaag gctccaccca tactcaaagc
    45601 gcgggcagtg gtgccaaaag tttgttgcat actgtagcgc aatgcatcat ttttgttttg
    45661 tgcaaaagct gttgcggtta aaaaaaagca tgccgcaagc atgcttttaa atctataatc
    45721 cataatgtcg ttaattgtta tttactgata attatcttcc gttgcgtgaa ttgtttctgg
    45781 aaggagttga ctcttgtcgt ggacgtgatt cctgacgttg ttgtgatggc tcagagaagt
    45841 tgcgttcctg acgtggtgcc ggtgttgctc tttcctgacg aggtgtgtgt tcctgacgtg
    45901 gaggattgtt tcttacagga gcttcctgac gtggagcagt ttcactgttt ctgttattgc
    45961 gtggcgcact ttcataattt ctccttggag attctgccgg tgtattgtag cgtggattgc
    46021 tctctgtgtt tcttcttggt gactctgcag gagtattatt gttgttacga ggattacttt
    46081 cgtaattacg tggaggaaca gtttctgtgt tgcgtggact gttgtactca ggattaactt
    46141 catttctgct gttgttgcgc accggaggtg cagcaggagt agttgagttg tcttttacct
    46201 cattattgtt gcgtggcgta gagaactgat cggctctgct gttttttaca ggtgctgttt
    46261 caacattacg agagtctttc accggactat tggtgttcac attgtttctt ggatgtatta
    46321 cttcagcatc attattcctt gcaggtgcag tttcaacatt gcgagagtct tttaccggac
    46381 tattggtgtt tacattattt cttgggtgaa ttacttctgc atcattattc cttacaggag
    46441 ctgtttcaac attacgagag tcttttacag gactattagt gttcacatta tttcttggat
    46501 gaattacttc tgcatccttt gtatcacgtg ttggtaatga cattgcattg gcaaaactct
    46561 gacttacagg cattgagttt cttccgtttt tcaccaaagc cctattgtac gaatcggcta
    46621 tgcttcttgg cggaacagca ttgctgctgc ctgaaccgga agttgatcgt cttggtccgt
    46681 aatatgtgtt gttgtagcta tagctgtcgt agctgttaaa atagtatgga ttgtagccat
    46741 aaccattata gtaaccatca taataaccat tccagtaccc gttgttataa ccattccagt
    46801 atccataact gtaattgttc cagccatgat gatgatatgg gccatatcca tagtatgaat
    46861 atggactact ccaccagtag ttgtagggtg tataacccca actgaaataa gtgaatgaag
    46921 gagcccacca tgatgaagga gcccaccagt tgtaaccgca ataaatgctc acaccatagt
    46981 cataaggatt ataatcatac cagtatgagt tggtgtaata ggaatcatag taaccatagt
    47041 tccacattgg gcgataaaat ctgcgtatgc gtgatgaata ggcatagtca taataatcat
    47101 catcgctgta ataattatta gtcacatagg tgttgccatc ctgtgttgtt gtgcctgcat
    47161 cgttgtaatc aaaacggttt tctgcagagg tgctgctgcc ctgatttgat acagattgat
    47221 cgcttacatc ctgctgaggt tgctgcactt gttgctgtgt ctgcacggtt tcagcaggtt
    47281 tgtttgcttt ggatgaataa taaacgtcat cggaataggc atttgaagcc tgttgtgatg
    47341 aagtgcagga agcaagtgta aatattgctg ctgcagatag tgctattgat ttcatcgtat
    47401 tttgattttt aatgtttgac atgtttttat acacaatagt ttaacgtaat tggatatgtt
    47461 atgtttgttt tacaattttg ctgccatctc ataaaattca acagttttgc aaaaatcatt
    47521 cattgcaatt tcacttcaaa gataaaaaat ggctaaagac tttccaacac gcgaacaaaa
    47581 ttattcagag tggtacaacc aacttgtcat tcgtgcaggc cttgcagaaa attcatctgt
    47641 caggggttgc atggtcataa aaccctatgg ttatgccatt tgggagcgca tgcaacaaca
    47701 gttagacagc atgtttaaag caacaggaca cagcaacgct tatttccctc tcttcatccc
    47761 aaaatcattc tttagcaagg aagcaaacca tgtagaaggt tttgcaaaag aatgtgccgt
    47821 tgtgacacat taccgtttga aacaatccga tgatggaaaa agtgtcgtag ttgaccccga
    47881 tgcaaaactt gaagaagagt tgattgtacg tcctacatca gaaacaatta tctggaatac
    47941 atacagaaac tggattcagt cctatcgcga tttacctata ttgctcaatc agtgggctaa
    48001 tgtggtgcga tgggaaatgc gtacacgttt atttttgcgc acagcagaat ttttatggca
    48061 ggaaggacat actgcacatg ccacagcaga agaagctgtt gaagaaacaa gaaaaatgct
    48121 tgatgtttat gctgaatttg ctgaacaatg gttagctatt cctgttgtta aaggtgtgaa
    48181 atctgctaat gaacgttttg ccggtgcatt agatacctac tgcatagaag cactcatgca
    48241 ggatggaaag gctttgcagg cagggacatc acattttctc ggacagaatt ttgcaaaagc
    48301 atttgacgta aagtttacca ccaaacaagg tgttcaggat tttgtgtggg caacatcatg
    48361 gggtgtctcg acacgactga tgggagcatt ggtgatggct cattctgatg atgatggttt
    48421 ggtgcttcca cctaagctgg caccaattca ggttgtcatt gtgccaattt ataaaaatga
    48481 tgagcaacta aaacttgtta ctgacattgc acttaaaatt aaaaaagcac tcaccgcacg
    48541 tggcattgcc gtagtttatg atgacgatga acaagccaag ccgggatgga agtttgcaga
    48601 atatgaaatg aaaggggtgc ctgtacgatt ggcaattgga ccccgtgatg tagaaagcgg
    48661 cacagtagaa cttgccagac gcgatacgaa agaaaaaatg actgttcaga taacagatat
    48721 tgaattgaag attgaaaatc ttcttgaaca aatccaacag aatattttta agaaagcagt
    48781 tgactttaga gaaacatcaa cgcatgtcgc tgatagtatg gacgagttta ttaaaatact
    48841 cgacactaag ggaggatttg tatttgcaca ttgggatggt acagctgaga cagaagaaaa
    48901 aattaaagaa caaaccaagg ctaccatccg atgtattcca ttaaacaaca aacaagaaaa
    48961 cggcaaatgt attcttacag gtaaaccgag tacacaaaga gtgctttttg cacgagctta
    49021 ttaaaatttt aaaactatgg aaccggtaaa cgataagata gcagaactca ttaaaacaca
    49081 ttttccactc aactgcaaga tagttgagtt tgcatcaggt cattttaatg agataaaaaa
    49141 ctatctgaaa aaatttgttg atggtacggc atcttattat caaccaccca acaagcgtca
    49201 gcgtataaaa ttttatgata caggcagcaa tcagtgtgaa ttgcaactgg gtgatgacac
    49261 gctatggttt catttacatc caaatttatt tcgtataagt cctgataatc ctgcttatcg
    49321 ttattcctat ctgcgcgatg atgaatatcg cagccgctgc gctgttattc acgtttataa
    49381 ttttctgtca gactctcttg ccgagcgcag gagtgatgac tatggtatgc tcgttgccag
    49441 aatttttatc aactacgaaa atcattttat ggttgaagga cgcaaacagt taggcatgtt
    49501 gtttaacgac atcagcaatg atattttgac tgaacaacgg ttgaatgatg tactcgatca
    49561 ggtaattttg ttttgcttac aatatgattt gtttgcacaa ccttttgatc aggttccggc
    49621 aatgagctat cgcgaaattt cttctggttc gtttgggtct gctgttgcaa gtagtaaaca
    49681 acttggcttc aagctgaagg ctgaagatca tgatacggag taaactttaa atgcagttac
    49741 ttaaaaaaac cttaattaca attgcaagta ttgatggtgt ctttaaattt ttatcaatcg
    49801 ccaattttta gatttctttt cagattttag tgtaatgatg taagagccat caggcaaagt
    49861 tgaaacatca acagcatact gatattttat gtcttttgtc attctgctga tttgttttcc
    49921 agagatgtcg gtaataataa tttcagcatt gtttattatt tcattcgtaa caagattgaa
    49981 aacactcaca gttggattag ggaatgttat ccaatttgaa actgaaaatt cattgatgcc
    50041 tactgtagta accgagtgac atgatgaggt gtcagtacat ccgtttgctg agatgataac
    50101 tgcatagctg ccattggctg tggctgtgaa agtttgattg gttgcacccg gtattggact
    50161 aaagttgttg ctgcaatcta cccattgata agttgctccg acctgcacgg ctgttaatac
    50221 tgcctgattc tgattcacag tagtgtcatt cgaaaaaagt gtaagtctca ctacagagtc
    50281 gcagtttgca gatgaaacca aagtagctgt gtaagtaccg gcctgtgtca gtgtttggcc
    50341 gttaaaagaa taagtttgac ctgagcaaat tgtatctgta atgatggttg cattgtcaat
    50401 gccatttaca aaatttgatg atggaccatt catgctaaaa ttttgtaaaa caccattgat
    50461 atggtttgaa atatcaggaa tggtaagaat accggaattg tttccacctg ctgtgccggt
    50521 gttcatatca taataaagtt gtaaaccggc agaagtagca tctaccttat ttttataaat
    50581 acataaaatt tcctgaggtg ttagcgctct gttccaaaga gccacttcat ccatttgacc
    50641 atcaagaaga aaattagtgc cactaaaaac aagattaccc atataaaatg atgtagcagt
    50701 gctggaaatt acaccggatg ctgaaacgga atcggtaatt aaaccatctc taaaaagtct
    50761 taaatatgag ccatcataag ttaatgcaaa ttgctgccat gtgttgagtt gaagtccttg
    50821 cgaaagaata tcaaaattta ttcctgcgct gtttctaaaa cgagcttcaa cggctgtgga
    50881 agaatactgc aaaacataga aatcggctac cgtattatct ctgaaccctg ccagtccgtc
    50941 aaagtcagga aatccaggaa caggattgcg tggatacacc caaaagctaa gtgaaatttg
    51001 cgatgaatta gcaatgagtg acgaggcatt tacagaaaca acctgatcgt ctaagccgtc
    51061 aaaatcaatc gcattttgac tctgagctga tacggaattt gtaaatgaat atatcagcaa
    51121 tacaattaca aaagcatata tttttttcat ctcctttatt gtttattaat ttttacaaat
    51181 ataaaaacat tcagtactta ttttcttgat tatgttgtag ttaaaattcg ctgttaccta
    51241 cggatgtatt aaaggcatgt aaattttaag taagaatttt tgtggcaagt cgaaatcgca
    51301 ttgattgtat aaactaatga aaagtaaaaa gtaattaaat aaagctgatt tattgaagtt
    51361 cttttctgca aagccgaatc aaaacattac ttttgcatcc cgtatcacca cacccctact
    51421 tatgagcgct gtaaagtatg tttttgtaac cggtggtgtt tcttcatcat taggcaaagg
    51481 aattatttct gcttcacttg ccaaattgtt gcaggcacgc ggttatagag ttaccattca
    51541 gaaattagat ccctacatca atgttgaccc gggcacatta aacccctatg aacatggaga
    51601 atgttatgtt accaatgatg gtgcagaaac agacctcgac cttgggcatt acgaacgttt
    51661 tttaaacgtc cccacatcac aggcaaataa cgttaccacc ggccgtattt atcaaaccgt
    51721 aattaataaa gagcgtgaag gagcattttt aggtaaaaca gtgcaggtta ttccacatat
    51781 taccgatgaa attaaaagac gtataaaaat attaggcgaa accggtgagt atgatattgt
    51841 aattactgaa attggtggaa ctgttggtga tattgagtca ttgccgtttg tggaatctat
    51901 ccgtcagatg atttgggaaa tgggtagcaa ttgttgcgtt attcatctta cacttttgcc
    51961 atacttaaat gttacaggcg aattaaagac taagcccaca cagcattctg taaaaaccat
    52021 gcttgagtat ggtgtgcagc ccgatatttt agtttgccgc agcgaaaaac atattactgc
    52081 cgacttgcgc agaaaaattg ctttgttctg taacgtaaat gtcaatgcag ttattgaagc
    52141 tgttgacgct agcaccattt acgaagttcc actgcacatg ctcaaagagc agttagacaa
    52201 agttgtgctc aataaactaa aactttcctt caagcaagaa cctgacctca cagcatggaa
    52261 agctttttta gggaaattaa aacatcctgt gtctgaagta aaaatcgggt tgataggaaa
    52321 atatattgag ctgaaagatg cttataaatc tattgccgaa gcatttattc atgcaggcgt
    52381 tgctaatgag tgcaagttga atattgagtg gatacattcc gaaaaaattg aagaagaaaa
    52441 tgttgccgaa aagctaaaag gcttgtcagg aattttggtg gctcccggct ttggcgacag
    52501 aggtattgaa ggcaaaattg cagctataaa atatgctaga gaaaataaaa ttccttttct
    52561 gggaatttgc ttaggtatgc aatgtgcagt agtagagttt gcccgcaatg tacttggact
    52621 gaaagatgca cacagctctg aaatgaataa agagacaaaa aatcctgtta ttgatttgat
    52681 ggcaaaacaa aaagaaattt cttcaaaagg tggcaccatg cgtttgggcg aatatccttg
    52741 tgcacttgca aagggcagta aggtggctca agcctatgga aaaactaaaa tctatgaacg
    52801 ccatcgccat cgttatgagt ttaacagcaa gtatcagaaa agatttgaag atgcaggcat
    52861 gattgccaca ggaataaatc ctgccgacaa cttagtagag attgttgaaa ttaaaaatca
    52921 tccttggttt gttggagttc agtttcatcc cgaatataag agcacggtag aaaacccaca
    52981 tcctctattt gtaagatttg taaaggcagc cagcgaattt gccaaaggcg ccattcagat
    53041 ttaaggcagt tgctctaatt cagtttttaa tgccggtaag gtaatagaat tcattactaa
    53101 cggtatggat acatcagtgc tgacgatgac agatgattta gaaaagaaat aattgtcgtt
    53161 gaccttcgcc aatgccataa ccgttacatg cagattgaca ggaaaatttt ctacttccca
    53221 cacgcctaag gtaacagatt tggctttgca gatggtgttg ctgccatcca cagaaacaaa
    53281 aacggcagta ttggaagttt cgtagtctgt acctgtgtca acagtaattt tttttctgct
    53341 gctattactg aaaaccggct ctgctgcatt ttgccaacct gtatttttca tcacataggc
    53401 atagtgcgac tgactggaag aagataggag attaatcatt tgtgtatcgg catcagcagt
    53461 ccatgaaaca tcactgctat tattttcaag aggtaatgag ctgctgtaat aacgatgcag
    53521 catttgcccg ttgccaaaaa cagcgttgag aaaaaagttg atggttttgc cattagacaa
    53581 acttaatgca acatcattct gatatgctgc tagataaaaa ataccacctg ttgtaagaaa
    53641 tttatttccc gattgtgttg tcagatttga aagaataata tcacgcttgc tgacacattc
    53701 acgcaccttt atatcaatgt ttccacttac agcactgcca tctgttttgg taaaacttcc
    53761 ggcagaaatc ataaaagtat ttcctgctgc tgttgtttgg ttaatgttgg ccgatgacga
    53821 aaaggaaaat tgctgcacag gagcccctgt ttcatcgaaa aattgctcaa tatcttttac
    53881 aggagtatcg tatttactgc cttccttttt gcaggataat gtagcaatac taataaatag
    53941 cataagcaat aaagcagaaa aacgatgatt cattttttag aactttaaat gcaataatag
    54001 aaaggtaaaa tgtataagtc aatagacttg caacagagtt ataattgatt tttacacatt
    54061 ttagaaaagc agaacatata aaaattagag tagtaagaca ttgagtgtca tcagttgtaa
    54121 gaaataatac tgatttttta cctcaacaga gtccctcctg atttttaatc agtggagtga
    54181 atagaccaaa gatagaaata tcataaagcg taggaactgt tatcttcgca gcatgtcaaa
    54241 accaacaatt ttagttacca acgatgatgg aattactgca agtggcattc gtgcattggt
    54301 agaagccatg cgcgaaatag gtcatgtaat agtagtagca cccgacagtc cgcaatcggg
    54361 tatgggtcat gccatcacaa tccacaatcc tttaagactt gataaggtag atgtgtttaa
    54421 gggagtagat gcctggcagt gtagcggcac accggtagat tgtgtaaagc ttgccgtaaa
    54481 taaaatattg catcgtaagc ccgatatctg tgtaagcgga atcaatcatg gaagcaattc
    54541 atctatcaat gtaatttatt cgggcaccat gtctgctgca atggaaggtg ctattgaagg
    54601 cattccaagt attgggtttt cattggtaaa ttataaactc gatgccgatt tgtctgcagc
    54661 caaacattat gcaggagtca ttacaaaaac tgttcttggc ggaggattgc atcataattt
    54721 attactcaat gtaaacattc cttatctgca gcttgatgaa atgaaaggat taaaaatatg
    54781 ccgtcaggca atggccaagt ggaaggagga gtttgatgaa agattagacc ctgcacaaca
    54841 aaagtattac tggcttaccg gaaagtttat caatcccgat ttgggcgaag ataccgatga
    54901 atgggctttg gcaaatggct ttgtttctgt tgttcctgtt gagtttactg ctcatcatgc
    54961 cattcccgta ttaaacaaat tgcagtggaa tgtttaagaa aattccggat acatttcata
    55021 ttggcgcact gtctgctttg gccttgtcag tgcttgcgtg gctgcttatc agccgcacag
    55081 aatcatatat tgtaacatct gcattaaaag attatctggc atttccacga ctgtatttta
    55141 tagtgtttgc cttgcagctt gtttttttaa gaataatgat gattaacttt caaaaacaac
    55201 actttgcccg tgggtggatg tttagtgttt ttatttttgc tctgtttgta ttttacctga
    55261 taaaaaaatc gtaatcatgt tcagtaaaga taagtttata acaggattca ttggtggcat
    55321 cattgcaccg gtagttggtt tttatctcta ttatattgca ctgttccgcc aaatggatct
    55381 ttccggattt tacaacacct tggtttcaaa caacacgctt gctgcagcta tcagtctgag
    55441 tttgcttgct aacttagcac tgttctttgt gttcgataaa tttggtaagt tgaatggctc
    55501 gcgcggtgtc attgcagcaa cattcattta tggattttac attgtttatc ttaaattttt
    55561 ctgaccccga tgcgctacta catcattgcc ggtgaagcct caggcgattt gcatgcatct
    55621 aatctggcat tgcaactgcg taaatatgat acaggcgcta taatgcgcgg atggggaggc
    55681 gataagatgc aacaagcagg agtggaaatt gtaaaacact atcgtgagct ggcatttatg
    55741 ggatttgtag aagtgataaa aaacctacgt accattttgc gcaatcttga aatatgtaaa
    55801 gaagatattc ttgcttttaa tcctgatgta gttattttag ttgactatcc gggtttcaac
    55861 ctgcgcattg ctgagttcct gaagaaaaaa aacattaagg tgttttatta tatctcacct
    55921 cagatttggg catggaaaca atgatgtgga caagatgttt gtaatattac ctttcgaaaa
    55981 agatttttat aaaaagtttg attatgatgt agtgtttacc ggacacccgc tattagatga
    56041 aattgaaaaa atcaaacatc aaccggctaa ggcattgtcg cagtcagata tagataaaaa
    56101 aattaatgca ttgtagccgg ccagtcgcat gcacgaatta aaagaatag
//
LOCUS       DAOOOI010000009        47281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_116_length_47281_cov_55.446343, whole genome shotgun sequence.
ACCESSION   DAOOOI010000009 DAOOOI010000000
VERSION     DAOOOI010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 47281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47281
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116_length_47281_cov_55.446343"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PKX59_02895"
     CDS             <1..320
                     /locus_tag="PKX59_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89844.1"
                     /translation="YFDFNDPVITNTATTHVVLPTGIASYQSAENIQLFPNPAQSELN
                     IQQIAAVEKIQVIDVLGKIVFEKKIKSQQNLKINISQFQNGVYFVKAYSAHDVVVKKF
                     VKR"
     gene            559..3213
                     /locus_tag="PKX59_02900"
     CDS             559..3213
                     /locus_tag="PKX59_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89845.1"
                     /translation="MRKLIILLLICTTTAHAQEIEWQNTIGGSGVEFLNYTQQTSDGG
                     YILGGRSNSGVSGDKTEPSKGDYDYWVVKLDATGNIQWQNTIGGDDADWLQSVQQTTD
                     NGYILGGWSKSNISADKTENSQGASDFWVVKLDSIGNIQWQNTIGGDSTDNLYSLQQT
                     TDGGYILGGFSISSISGDKTEYSIGGSDYWVVKLDASGNIQWQNTIGGYENDNLYSLQ
                     QTTDGGYILGGRSMSNATGDKTENSQGLDDYWVVKIDATGNIQWQNTIGGNSWDELES
                     IEQTSDGGYILGGSSDSDNSGDKTENSQGDFDYWVVKLDATGNLQWQNTIGGDNWDKL
                     KSIQQTTDGSYIFGGYSSSNISGDKTENSQGNYDYWVINIDSIGNILWQNTIGGLYAE
                     ALSSIKQTIDGGYIIGGQSVSPISGDKTEDSQGNNDYWVIKLTDKYNLIDGKMYADVN
                     NNTIQDVGELGVSNKMVTEQNTGRLSFSQQNGKYSLSVLDTGGFSVSASTINYYNSVP
                     LVHTAYFNNINQTDSLNDFALQPNGVINDVCINLTPVSLFRSGFDATYYISYINNGTT
                     TLTPTVIFFPDANVSFTSANVTPVSVTTDSVVWNIGALTPFQSGNIVVTVNVHTGLPI
                     GSLINSSVRIEPVAGDANPGCNYSSWEVFTIGSFDPNDILVSRDTLFTTEIPNPPYLD
                     YIIRFQNTGNDTAFNVKVLNPLDTNKLQLNTLEYVASSHPVNMNFIYHERNMEFKFDN
                     ILLPDSNVNEPLSHGFIHYRIKPKTSLVLNDSIQNNAAIYFDFNDPVITNTATTHVVL
                     PTGIASYQSEEKILLYPNPAQSELNIQLTTGVEKIQVIDVLGKIVFEKKTNSQQYLKI
                     NISQFQNGVYFVKAYSTQGVVVKKFVKR"
     gene            complement(3319..4248)
                     /gene="queG"
                     /locus_tag="PKX59_02905"
     CDS             complement(3319..4248)
                     /gene="queG"
                     /locus_tag="PKX59_02905"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOZ89846.1"
                     /translation="MNRSQTTAWVKAEALKLGFSHCGISKAGFLEEEAPRLEQWLTAN
                     MHGTMSYMERNFDKRLDPTKLVEGARSVISLLFNYYPEKKQNPQAPQISKYAYGTDYH
                     FVIKEKLFQLLELMRQHIGAIDGRVFVDSAPVLDRAWARKSGLGWIGKNNNLINPQSG
                     SWYFIAELILDIELDADAPIKDYCGTCTKCIDACPTEAIVMPHVVDGSKCISYLTIEL
                     KNEIPVSFQNQFKNWMFGCDICQDVCPWNRFAKPHQHQELQPKSELIEMTKHEWTELT
                     EEIFKKIFKDSALQRTGYKGIKRNLNFLKSEED"
     gene            complement(4255..5289)
                     /gene="ruvB"
                     /locus_tag="PKX59_02910"
     CDS             complement(4255..5289)
                     /gene="ruvB"
                     /locus_tag="PKX59_02910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOZ89847.1"
                     /translation="MNKNLDPASENLSTAEKEIERALRPLSFDDFAGQRKVTENLTVF
                     VKAAVQRGEALDHVLLHGPPGLGKTTLANIIANELNVKIKVTSGPVLDKPGDLAGLLT
                     NLQANDVLFIDEIHRLSPIVEEYLYSAMEDYKIDIMIESGPHARSVQLKINPFTLIGA
                     TTRSGLLTSPLRARFGINSRLEYYDARLLQDIVMRSASILKTAIDEEAAFEIARRSRG
                     TPRIANALLRRIRDFAQIKGNGTVNIEIARYGLQALNVDIDGLDEMDNRILNAIIEKF
                     KGGPVGLNTIATAVGEEAGTIEEVYEPFLIMEGFLMRTPRGREATEKAFKHLGKKSLK
                     QKGTLFDSES"
     gene            5436..7544
                     /locus_tag="PKX59_02915"
     CDS             5436..7544
                     /locus_tag="PKX59_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOZ89848.1"
                     /translation="MKGKLWRKQKIIIFRQRIFIGKRLDINVAMKRLIAVHIFMLLCS
                     AGQASHIMGGDFTYTLIGSVPQQQKKVYKISLNYYRNCDTSAAQFQPYYDLYICYDSS
                     NANYKRAYAQISLDKISTQPIVPPILDTTCYYNNNWCVEKTYYEATVTLSEDTTWHLF
                     LSDGARNVSINNLIQPDSTGIFFYTVIPAGISNSTPQFSNSPTPFICNLDTMQLSNLA
                     FDSDGDSLVYKIVQPYGYIGLNNLMTYQDINFNLYYLFPLPTVIYGPGYSYTSPFGAG
                     GLCSINSQTGLLTISSPNQGLYVLAIEVEEYRNGTLLSRTRRDMQVVVAACSYNTAPQ
                     LTSPVVLNYQIDEGDSICFPLTFTDAENNMINISATGNLFGTALTNPPAQFVVNPTPN
                     NNTAQFCWKTICGQAAPFPYMFTVESTDNGCPSKTNTYVFSIRVKTIPHNQLPSINLS
                     MYPDTPTCTGTAFQFNASTQFAGNNPTYQWYINGQALSNNYPNYTTNQLNNGDSVSVQ
                     LISNSICVIDSSAISNTQVATVLQPTVASFITSTVSVSSINVLQQFTNQSVGNTYNFW
                     NFGDSTQSAEINPQHVYENSGVYLVQLVTVSDDGCRDTTEAPVDIKTTMSFFMPSSFT
                     PDDNGLNDLFTPIGAEMPDYTLQIFNRWGQLLYSETGKPQWNGRYKNQLLPLGMYIYS
                     IRMHNPTDPIIKTGTVMLLR"
     gene            complement(7675..8454)
                     /locus_tag="PKX59_02920"
     CDS             complement(7675..8454)
                     /locus_tag="PKX59_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOZ89849.1"
                     /translation="MIVNNKIGITFSGGGFRGIAHIGIMQYLKELDIPIHAVSGTSVG
                     SLIGAFVAEGYAPLEILELAKREKFFTFSDLSIGNGGLFNTDIFEKVIKKYIPHDSFE
                     RLKMPLYVAVTDLTYAELLIFNQGSLSFAVKSSCCFPLVFHPVVYNNDRYLCDGGLLN
                     NFPVEQITATCNKSIGVHINPINKLEGKLGYKEIIQRIIRIATSNIKSAENHKCDIFL
                     QPDEINKFNTFDTGKIDEIYKVGYDYAKKFKKEFLELKKQV"
     gene            8714..10171
                     /gene="cls"
                     /locus_tag="PKX59_02925"
     CDS             8714..10171
                     /gene="cls"
                     /locus_tag="PKX59_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOZ89850.1"
                     /translation="MKWIFITEIIYVLIVILVCLRIIWDTGTTTKALAYLLLVIFVPF
                     IGIFFYFSFGINYRKRKMYSKKIFQDDVLAERFREQILQYSEKTYRENIDIVENNKEL
                     ARLLTYDSLSALTSGNEVKLLINGEQKFPEVLNAIASAKHHIHIEYYIFENDSIGKSV
                     IEALIAKVGEGITVRFIYDDFGSHALNRKILKRMKDGGINLFPFSRVRLFALANRLNY
                     RNHRKIIVIDGNIGFVGGINISERYINSEEQGKKLYWRDTHLRIEGAGTYYLQYLFLC
                     DWNFCAQSQLQPSREFFPSPTLLNNKGNKIVQIAASGPDSERPTILFSLLQAISLASK
                     EILITTPYFIPGESILDALMVAALGGVSVKILVPGISDSRIVNAAAFSYYDSLLNAGA
                     EIYLYQKGFIHSKTLVADDKIAMVGTANMDHRSFDLNFEVNAIVYDETFAKELKREFY
                     KDLKDAESIDVKAWNNRPIIKQLGEKCARLISPML"
     gene            complement(10191..10793)
                     /locus_tag="PKX59_02930"
     CDS             complement(10191..10793)
                     /locus_tag="PKX59_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOZ89851.1"
                     /translation="MNAECTPFNSNDKAVENIIVNRARHLFMKVDIKSVSMDDIANAC
                     GISKKTLYKCFDKEGLVKTVTQQQVHIFENEIKSILQNNKDVVQELTNLLLFTINASQ
                     EISLPFIDSLKTIKPEAYQLILNFTKNIQQTFINNIKKGMSQGYYRSDIEPDVVVQLH
                     FLQLWSLMKQQMLSKSLHWGEELNKMFLFGILSPKGLKTL"
     gene            complement(10806..13994)
                     /locus_tag="PKX59_02935"
     CDS             complement(10806..13994)
                     /locus_tag="PKX59_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOZ89852.1"
                     /translation="MKITEISINRPTMVVVVFTILGILGLISYSSLNYELLPKFSTSM
                     VTISTVYPGAGPDEVQNTVTKKIEDAVSSMENIKKLTSRSMEGVSIVTVEMNVGADAN
                     RSLEDAQRKINAMLSDLPSDAKTPTLSKVSVDDLPIITLSVTADMDSREFFDLLDQRL
                     APRLSRLPGVAQVQMIGGTEREFQINVDAGKLNAQKLSLLQLRQTITNANVDYPTGKI
                     QTTDNLIAIRLSGKYADVDMLRNLVIKTSDDGSQVRLKDVADVRDGQKDVNKLARQDQ
                     KETIVVQITKQSDANAVTVSKEVHNQIAGIEKEYASNNLKMFVASDSSEFTLESANDV
                     VFDLFIAIILVAVVMLLFLHSIRNSLIVMISIPLSLIITFAGMYVMGYSLNLMSLLAL
                     SLVVGILVDDAIVVLENIYRHMEMGKNKVRAAFDGVKEIGLTVTSITLVIVVVFLPIS
                     LTNSLVSMILRQFCITVVISTLLSLLVSFTVVPWLSSRFGKLEHLTGKNMFQKFILWF
                     ERQIDKFTEWITGILRWSLGHKRYVLIGTIVLLVASFMLLAKGFIGGEFIPKGDRGQF
                     IVTIEMPKDVALEVTNQMTMRAENYLAKQDLVKSLTTTVGKSSDGGFGTSSSTPYKSE
                     ITVTMIDENLRSEPSSMYAAKIKKELAAQLAGAKITTSPVSIMGTAERASIDLVVTGT
                     EQDSAMAFAKKALLALQKIDGVSDPKLSAEDGNPEVRVTLDRDKMALLGVNMDVVGGT
                     MQTAFSGTDQDSKLKFREGEYEYDINLRYDNFNRKNADDVRNIMFVNNKGQNIRLSEF
                     ATISETSGPTVLERRDKLASVNVQAQVIGRPAGSVTTDFTESLKKLDKPAGVNYIFGG
                     DAENQGNAFGTLGLALLLSIIIMYLIMVALYDDYVRPFVVLFSIPLSLIGALLALALT
                     NNTLNIFSILGVIMLIGLVAKNAIMLVDFANQMKEKGESTDEALVHANNARLRPILMT
                     TIAMVIGMLPIALASGGVAAIKHGLAWVIIGGLISSMFLTLIVVPVIYKIMDSVSARF
                     GWGDLNKKKIVRELMHAENTNTADNHSISHVI"
     gene            complement(14032..15093)
                     /locus_tag="PKX59_02940"
     CDS             complement(14032..15093)
                     /locus_tag="PKX59_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ89853.1"
                     /translation="MKKVFIYGGTLIVALALIVWKLNANKKSDAARIEVVRESSSGAV
                     PVQILAVKSSNVDLSFDENGNFEAVNQIELTAEIAGRVSELYVKEGSVVKAGQAIAKI
                     ENEILNANQTEAKARLEQLRLNLNRYEQAYKTGGVTQKQVDDARLELESAQAKNVQAN
                     KNLYNSVVKAPVNGIINTRYVEVGTYLASGNKIVEIVDDAKLKLTVNVNEYQIIQLHT
                     GDKVEVTASVYPEVTYQGVITFIAAKSDASLNYPVEVEINNIAGKALKAGMYGTAHFK
                     PEIKNASLMIPRSAFQGGINSNLIFINDNGKAKLQKVVAGNVHGNMVEIREGLKEGDQ
                     VIVTGQINLVDGKQISIVK"
     gene            complement(15122..16483)
                     /locus_tag="PKX59_02945"
     CDS             complement(15122..16483)
                     /locus_tag="PKX59_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOZ89854.1"
                     /translation="MKLSIKNNALAIAIMLFSTQVMAQTKSLNLKDLIQIGLEQNYNI
                     QLSKLDEAIVEKQIVETRGLTLPQVNGTGNITNNYKRQVLVLPAGLGGGTGEGSRKIE
                     AGTNYSSALGVEATQALFDVAALQGLKAAKAGRVYARVNTQQTQEQVVNDIAQQYYQI
                     LSSMEEVKLQHYTIEIMQQLVQASEGKYNNGLLRKVDVDRLKVNLINAQSKLTQAQND
                     VDVKTNQLKVTLGLPYNTSILLDSMKIDETSLQVALQQQQYNPDASTDLKLLDTQIKL
                     STLERNATRAANYPKLNAFANYNYNMASDKFSDLYKGTDPAISYGMGSVGVRLSIPLF
                     SGFSRSSKVAQNNIKIHQLEKQRQATAITLEAKNESARIQMENTLTTARAQQENVKLS
                     ETVYESNRSNYTLGLSSLTDLLDSQTSFLEAKNIYTKSIINYKLAELELMRSNGTILN
                     LIN"
     gene            16807..17898
                     /locus_tag="PKX59_02950"
     CDS             16807..17898
                     /locus_tag="PKX59_02950"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOZ89855.1"
                     /translation="MITSKTKWIEFINKYYKYFLIPIVFFTHKLLFFLTDPYAQPIRK
                     LTFQESFIQNLGLLFFSFILVYGSVRNVNRLNKRLPWAASPILRLIAQVFVQLMLSTI
                     WIFIYQFVVVKMLFDVNIFSLITSPDLTPGIRFVIWRFFFTSALVSLFVSLIITGRNF
                     RMETAEMKIQAAQLKQIAAQAELQALKLQLDPHFVFNNFSTVSALIEEDKALAQSFVE
                     NLSRVYRYMVQNIHSDTITLQREVKFIESYIYLISIRHGDNVRIKVNIPEEFLQKRIP
                     PITLQLLIENAIKHNIASHTKPLNINIGVADDMLRVSNNLQRFSNPSQISTRIGIENI
                     KSRYRIVSGKEPVVIETADDFIVELPLLD"
     gene            17911..18669
                     /locus_tag="PKX59_02955"
     CDS             17911..18669
                     /locus_tag="PKX59_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOZ89856.1"
                     /translation="MKFLIVEDEKPNANRLKKIVEQLDSSFQVVDIIDSVAGAVDWFN
                     KNPHPDIVLMDIRLADGLSFDIFSQVKISSAVIFTTAYDEYALQAFKVNGVDYLLKPI
                     EIEELDAAISKVKQNHISASNNPYLDELIGYIKQKEVQYRSRFLFPYKDGYKTINVSD
                     IDLIYSEDKNTHLILQNGSVQIANQTLEEMEEQLDPAVFFRANRQYIININSIESIHN
                     HFNGKLKVVVRKLPQHDILISKEKAPLFKQWLDS"
     gene            19448..20200
                     /locus_tag="PKX59_02960"
     CDS             19448..20200
                     /locus_tag="PKX59_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89857.1"
                     /translation="MLKRFVCITISLLAFIIVNAQDNKTEKKDSIEHRILLIPYDPRF
                     YLSDADKDIAEETRKDPKTIRNYFHNRSEWFTSRELKRHYPTISLLQNDTVPAYIQSA
                     GDIFSQTAFDYATPVTRSPESMMNATSDKTLTDSRTATNYLNTDVHNRYMKADIQNKK
                     VLADLSERFNTDIFVFLTQFEIKTNYKNCLDIANKIYEREIRLHFTIYDKTGKLINGN
                     YAIAVIPSNVNQMDEIILKCFPLLAKGIAASL"
     gene            20282..22528
                     /locus_tag="PKX59_02965"
     CDS             20282..22528
                     /locus_tag="PKX59_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HOZ89858.1"
                     /translation="MRKLLTLSALFVILSGTQSLASSFWTAKSETQEMLSGKRQIVPD
                     KYKVYTLDVQGLKNQLLNAPMEFTMAAKQSPVVITLPMPDGSWNSFSMVESPVMQPKL
                     AAKYPFIKTYSGSGINNQPSWVRIDFTQWGFHALIRTAENDIYIDPYSMQTTSDYLVY
                     FRKDFTKSQSSMVCEFDDLQQGQSANKTLTNSMPSVNRSIGTNLRTYRLALACTGEYA
                     AYYGGTKAGALSGMVTSVNRVTGVYESEVDVRLNLIANTDTLIFLNANTDPYTNNSGG
                     TMLGENQSVVNFFIGTPNYDIGHVFSTGGGGIAYLGCICKTADKAKGVTGSAAPINDP
                     FDIDYVAHEMGHQFGGNHTFNSVTGSCQGNREAGAAYEPGSGVTIMGYAGICGSDDLA
                     AHSIAYFHTKSFDEIVDYSTLSTGNSCPVNTITFNNAPTVSGGGIFNVPVGTAFKLNG
                     YGSDPDGDSITFSWEQFDLGPAGAWSLPTGNAPMYRSYAPSTSALRLFPKLSVLLNQV
                     NAKGELITSYARSLHFRLTARDNKPNGGGVTYNDTVTTVNVVQTSAPFAITYPNVTGI
                     SWPALSTQTITWEVGGTDAAPFNEQFVNIYLSINNGTTFPITIATNVANNGSYTFTVP
                     NNPSNICRMWVEGASTGSIFFDINNKVFTITAPVGINEVNSLSQLTTYPNPADNELLV
                     YLGNNYHGQLNISLYSVNGQLVLSQTIAAKNATAQVALNVEQLPAGLYFLKAETENGT
                     AERKVMIK"
     gene            22645..25950
                     /locus_tag="PKX59_02970"
     CDS             22645..25950
                     /locus_tag="PKX59_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89859.1"
                     /translation="MKPFFKAVFTLLFTFIILHSADAQFYQGSQMSFGKNRVQYTDFY
                     WTFFRFKNYDVYNYVGGKELATYVAEQAQNEMDVMERTLDYRSSSRFQIVIFNKLSDL
                     KQTNIGLELDDQAYNIGGMTKVIGNKVLIYFDGNHQHLQEQIRAGMARALLEQLFYGG
                     NIRERLQASILLNLPQWYTQGLVTYLSKGWTPEDDNKLRDYIMTEKKPVLNKLIERDE
                     VFAGQSLWHYIITTYGETSLSNLVYMTRINRSLESGFVFVLGVRLKQLSADWLSYHTK
                     KYQEFEKNKFPIAATPLKVKEKNKIIYQVKTDPFGNRVSYVTNKRGRYKVKITDVKTG
                     KRTCADKGGYVSLTTENDRSFPITAWHPSGKILAIVKEQKGNIILNLFNTETKKSDFS
                     EIMSFSKILNMTYSPDGSKLLMSAVQKGQSDIYVFNVRGRNFDQITNDVYDDLYPQYD
                     ITGNKIIFSSNRSNDTLGVDVKQNTPQNNFDLFMADLYNREGNLLRITHSPENETFPV
                     MSDSLRISYLSDKNGVINRFVSEMDSTISFIDTTEHYRYFFRHYALTNSNRNILEQDF
                     SARKNKFTELAVNNGRYRIYLKDNMPFDKSKTFDLSYIAQPVFQPKQENKPKVAETPT
                     RVVTIEPSQKKDSAAIDVGNYVFQSEFKSKKKKEEPIENKSVATDTTKIVQEQVKLQP
                     DSNDVKSTPDSAIFRMPKQRNYDISFSSDYILTQLDNSLFNTTYQPYTGGAVYFDPGM
                     NALFKIGMSDLMNDYKISGAFRIGFDFNSNEYMLMIESLKKRIDKQILFSRQSKLFSV
                     SDLDVLRTHTYNLKYSGKYPFSEISAVKGTIDARMDKTIYVATEKAQLEKSDITNYWA
                     SIKGEYIFDNTINKALNLYNGTRFKIFAEVFRKINKPEKMVYIIGADFRHYIKVHRQI
                     IWANRLSASTSLGKQKLIYYLGSQDNVIVPGNNFNNEISVDKNEEYVFQTLASPMRGF
                     KQNIRNGSSFALFNSELRIPVFQYLLNRPIRSDLIRNFQAVVFFDAGTAWSGTSPFSD
                     KNGLNEEVIGGGGSNPVTVTVIKQIQPVVGAYGFGFRSRIFGYFIRADWGFGIDDRQV
                     QKPLFNLALGLDF"
     gene            complement(25951..27213)
                     /gene="rodA"
                     /locus_tag="PKX59_02975"
     CDS             complement(25951..27213)
                     /gene="rodA"
                     /locus_tag="PKX59_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOZ89860.1"
                     /translation="MRKQKSVFQNIDWLLVLLYLVMVIAGWLNIFAAVYNEEHSVITD
                     FSQNYGKQMMWILTSLVIAFFILIIDGKFYDAFAGVIYAISIISMIAVLFFGHTVAGS
                     KSWFRFGDFGIQPAEFAKFATAMMIAKFLSPMGLKKNTWRTRIYAVAIFFIPLLLILL
                     ENETGVALVFLSFCIVLYREEIIPGWLLMIGISAAVLFVVSLLVPKMILVTSLLVLAA
                     LIVLLFRKWKTFFVVAGLVAICIGVVVSVDHVFNHVLEPHQKKRIQVFLGMEEDLKGA
                     GYNVNQSKIAIGSGGLTGKGFLKGTQTKYNFVPEQSTDFIFCTVGEEWGFLGTTTVIL
                     LFLALLYRIIFIAERQKSAFARIYGYGVAGVIFFHFMVNVGMTIGLMPVIGIPLPFFS
                     YGGSSLWGFTILLFILIKLDSYRTFVLR"
     gene            complement(27217..29058)
                     /gene="mrdA"
                     /locus_tag="PKX59_02980"
     CDS             complement(27217..29058)
                     /gene="mrdA"
                     /locus_tag="PKX59_02980"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOZ89861.1"
                     /translation="MKTSHSTQRLVLLITIALVAITYIGRLFYLQVIEDKYKLSADEN
                     VLRRIIDYPSRGLVYDRNGKLLVFNQTVYDLMITPREVKEMDTAEFCSIIKISIEDFR
                     KKFQKAKNYSPYRSSIFEKQISAETYGTLQEKLFKYKGFSVQPRTIRKYPYNTAAHLL
                     GYLGEVDENVTAKNNYYQMGDYIGISGIEQSYEKELRGRRGTRYMMVDNFNREKGRFK
                     EGKYDTLAISGENLKLSLDAELQEYAELLFQNKRGSAVAIDPSTGEILAMVNSPTYNP
                     NLMVGRERSRNYGRLLKDEDKPLFNRAMMAYYPPGSTFKIINSLIGLQEKVITPETYF
                     SCAGGYRVGNIFVHCHPHSSPLDLRTAVAVSCNSYYCQTFRGVIDNRKYNRTEDAFDA
                     WRNYVLSFGIGKKIDVDMPHELRGNVPTVDYYNRFFGKGRWKSSTIISLAIGQGELGI
                     TPLQMANAACIVANKGYYYVPHIVKEIGYKHYLPELFKKKNYTLVDPKYFDAIQDGME
                     WVFERGTARGSRVDSLVMCGKTGTAQNPHGKNHSVFFCFAPRENPKIAVAVLVENAGQ
                     GAHFAAPIASLMVQKYLLRTIKRPDLEKRMLDAVIVPMANDSTVLDD"
     gene            complement(29070..29588)
                     /gene="mreD"
                     /locus_tag="PKX59_02985"
     CDS             complement(29070..29588)
                     /gene="mreD"
                     /locus_tag="PKX59_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOZ89862.1"
                     /translation="MLPEVIKSVLRFFLLILLQVILLNKIRFAGYINPFVYPLFLLLL
                     PVRIPKTLLLILAFITGISVDIFSDTLGMHAAASVFLGFMRPYIIKLLSPRDGYDSDA
                     VISLKQMGILWMVYYLFISIFLHHLFLFYTEVFRFNDFGATFLRVCLSTITSVVIIFI
                     ILLLFDKTKVQR"
     gene            complement(29569..30369)
                     /gene="mreC"
                     /locus_tag="PKX59_02990"
     CDS             complement(29569..30369)
                     /gene="mreC"
                     /locus_tag="PKX59_02990"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOZ89863.1"
                     /translation="MFILLEAVCFFLIAQNNSYQNAATLNSTNKVVASVYSTISSVTD
                     YLDLAENNRRLAEENAHLRTLLPASYFDTTVVRNHKVDTIHNLEYSYIEAKIINNSTN
                     RRNNYMTLNKGSDAGIAPEMGVITGHGIIGIIKNVSAHYSTVTSFLHTQSKVSVRFKN
                     NNYNGSMVWDSDNSPTEATVIDIPRHVPFKVGDTLVTTTFSPYYPEGIMVGTVKSFDI
                     DAGSNSYRIILKLSTSFNDVSYVYVVNNLLKNERKQLEEKHKDATRGN"
     gene            30555..30830
                     /locus_tag="PKX59_02995"
     CDS             30555..30830
                     /locus_tag="PKX59_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOZ89864.1"
                     /translation="MLAVDYFRNINFNSAILEKKLSELKPGEAGVIQNFSDEEIKLKL
                     MEMGCIPGETVLMERVAPLGDPMAIKVAGYQLSLRKNEAETVWVSTL"
     gene            30848..32971
                     /gene="feoB"
                     /locus_tag="PKX59_03000"
     CDS             30848..32971
                     /gene="feoB"
                     /locus_tag="PKX59_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOZ89865.1"
                     /translation="MSEVVLKVALVGNPNSGKSTLFNVLTGLNQKVANFPGVTVEKHV
                     GFFSLRNAFEGVDYKVELTDLPGIYSLYPKTLDEQIPYKVLTDPQDPSFPNRIIVIAD
                     ASNLKRSLFLCSQIIDLKIPVVLVLNMMDLAVHKGLLPDVKKLEQALGIKVIPAIVRQ
                     KTGIEEIKKAVLHTIPLPESDIMPATTLAPELIQEIKQSFPVKSDYAAYLLAGNYDKV
                     DISFVGNEGKQKLDMLLQKHQFSQKTMQATETLERYKTISVLLKSCVSEPASVRKSLT
                     RTVDNLVTHRIWGYGIFLAILFVIFQFIFNVAQFPMDFIDGTFADFSQKVSELLPKGV
                     LNDLIVNGILAGLGGIIIFVPQIALLFLFITILEDTGYMARVSFIMDKLLRPFGLNGR
                     SVIPLMSGVACAVPAILATRTIQSWKERIVTIMVTPLMSCSARLPVYTLLISLVIPRY
                     YVFGFINSQGLVMMLMYLIGFVAAIFSAWLFKMFVKAKQRDFFIMELPVYRLPRWKNI
                     FFVIFDKVKIFLFDAGKIIIAISIVLWFLSSRGPGKDYAETEKLYEAKAELPETTQDE
                     LNQLQAQKLEHSYAGWTGKIIEPAIKPLGFDWKIGIALVTSFAAREVFVGTMSTIYSV
                     GSEGENLPIREKMMAEKRPDGTPLYTLAVGISLMLFYAFAMQCMSTLAVVKRETGTWK
                     WPIIQFVYMSALAWVSSFAAFQLLS"
     gene            32999..33628
                     /locus_tag="PKX59_03005"
     CDS             32999..33628
                     /locus_tag="PKX59_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sprT domain-containing protein"
                     /protein_id="HOZ89866.1"
                     /translation="MAKVTLEFIEEALKKYVPHQSIRYCIQWIEEHQINLKITRSRNS
                     KYGDYRPPQRGFGHRISINHDLNPYAFLITFIHEVAHLNQWKRKKQIIIPHGKDWKEE
                     YRKLMIPILRENIFPPDVVAALNNYMTNPSATSCSDHDLLRALRNYDREEDKWITLEE
                     LNFGVKFKIRTGRTFIKKEKLRKNYSCIDCRSKSIYFINPITEVMPVDH"
     gene            complement(33622..35034)
                     /locus_tag="PKX59_03010"
     CDS             complement(33622..35034)
                     /locus_tag="PKX59_03010"
                     /inference="COORDINATES: protein motif:HMM:NF017754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ89867.1"
                     /translation="MKPAAVFNLLLKNFPFEPTNAQKHAFAGLSEFITENTAYKCFLL
                     KGYAGTGKTTVINAMIKTLYHYDFRVIMLAPTGRAAKVINHYTGDDASTIHRYLYEIK
                     SDKNGDIKTKVKPNKFKKQIFIIDESSMISNEDDKTLFKGASLLSDLVDHVYSQADNA
                     IVFIGDTAQLPPVGLQCSPALDQNFLQNNCQLQVRQYELKEVVRQQLHSGILANAHCT
                     RLLLEREEHFPRLKTSGFDDVNQVTSAELSEALSGYFPSGDDNTVTVLCRSNKQANRY
                     NEFIRKTFYGFEEVVEKNERLMVVKNNYYWHESDNDNAFIANGDEIKLIRLKKQEEYF
                     GFRFANATIELSDEKSTRLEVKLLLDTLNSESPALTYDEYKKLFDEVRNHYRKTDGKV
                     NMRKLREDPYLNALQVKYAYAITGHKAQGGQWETVFIDPGFMTEEMYGVDYMRWLYTA
                     ITRARKKVYLINVPDRFFVQ"
     gene            35246..36259
                     /locus_tag="PKX59_03015"
     CDS             35246..36259
                     /locus_tag="PKX59_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOZ89868.1"
                     /translation="MLSIDIHTHILPEKLPDWKSKFGYGGFITLDHHKNCCARMLRDD
                     GTFFREIESNCWDAKTRMNDCDVHGVHVQALSTVPVMFSYWTKPADGLAVSEFLNDHI
                     ATIVQQYPKRFVGLGTVPLQAPDLAIKEMERCKKIGLQGLQIGSHVNDWNLNDANLFP
                     FFQACAENNMPLFVHPWEMMGQKDMQRYWLPWLVGMPAETSRAICSMIFGGVFERLPN
                     LRVAFAHGGGSFPSTFGRIKHGFDCRPDLVAIDNASSPERYIGKFYFDSLVHDENMLH
                     YLINFTGANRIALGSDYPFPLGENIPGTLIKNAKLDLETKERLLGGTALEWLGLSKEM
                     FLR"
     gene            36385..36999
                     /locus_tag="PKX59_03020"
     CDS             36385..36999
                     /locus_tag="PKX59_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOZ89869.1"
                     /translation="MDIRERILESGLELFLAHGVRRITMDDIARHLSVSKKTIYACFD
                     DKDEIVSECCQGYLKNSRCEFEHVETESKDAIHEIFLTMEKLQAMFTTINPALFEDLQ
                     KFYPRVWSMYVQFKDKHVAEVIERNLLKGIEQKLYRPDINIKILVKLRIEEITLGMNT
                     TVFPLKNFKTQDVQVALLDHYLHGIVTVKGHKLINKYKNITDEE"
     gene            37011..38360
                     /locus_tag="PKX59_03025"
     CDS             37011..38360
                     /locus_tag="PKX59_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOZ89870.1"
                     /translation="MLNQIKFLTGLLLLISINIQAQQSDKKSYSFSLKQAIDFAFQNQ
                     TDIQNAVLDQKSAQSRANELLGIGLPQISASADITHFIDIPTTFVPAEFFGGEKGSYA
                     PVQFGQPYSSTAGLSASQLLFDGSYLVGVKASRMYADLMRKQVIQTKTEKAALISKAY
                     YNVQVNSERLQMLDANVKRLKKQLDEMQVMYNNGFIEKIDLDRISLAYNNIETEKSNA
                     DRLIVLAQNLLKFQMGLNVNDELTLTDKLEDIKVVADNNLNQNIDISNRIEMDVLNTT
                     KELLRADLRLKKSSYLPSLAAFGSYSVSASRNEFDIFENKPWFRTSIIGLKLNVPIFD
                     GFQKAARVNQARFALNKMENQIQTATQGFQLEAQTAKTTLTNALGSLANQEKNRTLAA
                     DVARITKIKYQQGVGSSLEVMDAETALKVAETNYYAAMVDAFIAKVDFEKATGTLIK"
     gene            38388..39506
                     /locus_tag="PKX59_03030"
     CDS             38388..39506
                     /locus_tag="PKX59_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ89871.1"
                     /translation="MKKVIIAITAIGFLTACGGGNSSKKVEELEKLRKQQSELNEKIK
                     SLEAEIALTDTSRKDKIKFVAATDMSLKPFTHYIEVQANVEGDEDVVLSAKTPGTVTS
                     VNVKAGDHVTKGQVLATLDDQIMQKAIAEVQNELNLVTTLYNRQKNLWDQQIGSEVQY
                     LEAKTRKEGAEKKLASIQEQWDMTRIKAPFSGTVDNVMIKTGQLVSPQVPAIRVVNLE
                     MLKVKGELPETYIGRVNKNDNALVYFPDLGKEITSKVYYSGNAISNLNRTFNVEVRFG
                     SKENFLRPNMLAVLKIADYNKEKAMSLPVSVVQKGLDGEYVFVAENSGGKTVAKRKTV
                     KSGMTYNGNVEIVEGLNEGDKVITTGYHDLVEGDIIKL"
     gene            39521..42913
                     /locus_tag="PKX59_03035"
     CDS             39521..42913
                     /locus_tag="PKX59_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOZ89872.1"
                     /translation="MKDIFKEFKPSSWAIDNRTAIYIITVIITLTGIMSYINIPKESF
                     PDISLPNIYISVVYPGTSPKDMENLVVRHIEKECKGISGVKKIKSNALQDYCSIVVEF
                     NSDVKIEDAKRKVKDAVDRARNDLPQDLPNEPDIIEVNFSDLPIMAVNLSGDYDLAKL
                     KRYADEAKDKIESLKEVKEVKLIGELEREIQINVDMLKLQSSGLTMSDIERAVKYENM
                     TVPGGGVRMADGTRRSLSVSGEFTNVDQIKNLVVNSINGAPIYLKDIAEVVDSYKEQE
                     SYARLNHKNVITLSIIKRSGENLIDASDKIVSVVNELKAEAWPKDLNVVLTGDQSEQT
                     RTQLHDLINTIIIGFVLVLIVLMFFMGTSNAFFVAMSVPLSMCVAFLVLPVIGFTINF
                     IVLFSFLLALGIVVDDAIVVIENTHRIFDNGKMDIVRAAKMATGEVFLPVLSGTLTTL
                     APFVPLAFWKGIIGKFMFFLPITLIITLLASLVVAYIINPVFAVSFMKKHEEKHDPYK
                     GLKITSIIFIAIALLFYVTGAFGMGNFTIFILLLNLLHRFWLHKVIVRFQTKTWPAVQ
                     ESYKRMLIKVLHRPRTMMAGTVGLLFLSFIAIGLRNGGVNFFPIADPNFVYVFTTLPV
                     GTDQAYTDSVTKILEGKVYNVLGEKNDVVKSVIASVGVNVSDPSDEDQGYYANKSKIE
                     VAFVEYSKRHGVSTSNYLEKIREAVKNTVPGAEVTVAQEQGGPPVGKPISIEVSGDDL
                     QAITTTSKSLKRYLDSLQIEGVEELKSDLQDSKPQIVFDINREHMNRLGIMTGTVGNE
                     INMAVLGREASKYRDLNDEYKIMLRYKLDQRYDVSLLNNLKILYRDMSMGGLVRNVPL
                     SSFATVKYDNTYGVIKRKNQKRVVTVSSNLLTDYQSREAEVVSNVMAAVKQFPKTEGV
                     EIKFAGSQEEQAETMSFLGTALMISLGLIFMILVIQFNSIGKPVIILSEIVFSIIGVL
                     LGFAIFKMGFSIVMTGIGVVALAGIVVRNGILLVEFTELLREQGVPTFEAIVEAGRTR
                     MTPVILTATATSLGLIPLAVGLNIDFVKLFTEFNPHIFFGGDSTAFWGPLSWTMIFGL
                     MFATFLTLFLVPVMYLMSENLKAKIYKRPKPLMKKPDDELIA"
     gene            complement(43167..46253)
                     /locus_tag="PKX59_03040"
     CDS             complement(43167..46253)
                     /locus_tag="PKX59_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOZ89873.1"
                     /translation="MKQYTKFNNISGWAVFAIAAWVFLSTIEMTGSFWDCGEFIAAAY
                     KLQIGHPPGAPLFLMLSRIMTLFAGADVKDVPLMVNAFSAIMSAFAVLFTFWTITAIA
                     RKIVFVKNQEPTLAQILTVVASGVVGALAFTFTDSFWFSAVEGEVYATSQFFTCIVVW
                     AVFKWENVADEKHSIRWIILIAYLMGLSIGVHLLNLLCIPALALVYYFRKYPFSWMGV
                     AKTMGIALVILMVVQYGIIAGFVNIGAKIDLWFNQSGSSLWTGFVVYSLIILAALTYG
                     IYYSQKKQKALLNTILLSFAFLLLGYTSYAQIVVRSLANPPMDENNPENAFALLGYLN
                     REQYGDRPLGYGQYYDAKVVGQTEGAMSYTAIDGKYIPTGAKVIPEYDPARCTVFPRM
                     YSREPNHVSAYKEWSGIKGEQKPEFSNNLKYFFSYQMNHMFWRYFMWNFVGRQNDIQG
                     HGSILKGNWISGIPFIDSMFLGPQDKLPDSMKNNKARNTFYFLPLLLGLAGLVYHYKK
                     DMNNANVVMLLFIMTGLAIVIYLNQTPYQPRERDYAYAGAFYAFCIWIGLGVAAIADA
                     LKKRMPETTAAMLATLLCLVVPTVMAKEGWDDHNRSYRYTSRDFAYNYLNSCAPNAVI
                     FTNGDNDTFPLWYIQDVEGVRTDVRVINLSLLNTDWYIDQIKRKAYESDPVPFTLNTK
                     QYVQGTRDYIPFYDRSVQGYSDLKDIIEFITSDNPEAKVRSEGGDEFNYLPTKKFRIK
                     VDKQAVIKNNVVEAKDTSKIVDYIEFEISKNYIMKADLMILDLIAHNDWTRPIYFAVT
                     VGNDSYLNLENYFQTEGLAYRFVPIKAAADPSGQTGSVAAERMYDNMMNKFMWGNMKD
                     ERVYLDQNNLNMTMNFRNNFARLSETLLQENEPDSAVKVLDKCLVEMPDKTVPYNIMM
                     LRIAELYYRSTMSNALPDSLQMLSNDVELKKTDSKESIEKGNSIVKRLADIYQNDLQY
                     YLSLKGTPYAKGVERETNQALAVFQELIRLSKLKDQKAITDDLQKRFDQLEAQYSR"
     gene            complement(46373..>47281)
                     /locus_tag="PKX59_03045"
     CDS             complement(46373..>47281)
                     /locus_tag="PKX59_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89874.1"
                     /translation="PYQAIWGVIELPDHSFMCTGNNINTINGDLDAFYFYVDSFGNQL
                     SFFTIPTIGNSAFTEMIYSNDTNIIIIGSENFSSGSVQPYFNSIAKIDLAGAMIWKKN
                     YLPFNSIGSSLGLANAFNGGYIYAGLSDNMSNLAKAYIHCLDENGDSLWYREFGSSSY
                     SDFWDIKTTADGGYIMCGQTGGCNNNGGTCFWLVKTDSLGLLVTGIKEKEIFKTATLT
                     IYPNPATTYCTITAIVPQSNSTGIGKKGASIILFDMRGKQLLEQPLQTGSNTVQLNLT
                     NYTSGTYLAVLVLDGYNAARVKVVKQ"
BASE COUNT        15124 a         8494 c         8695 g        14968 t
ORIGIN      
        1 tttattttga ttttaatgac cctgtaataa ccaacacagc aacaacgcat gttgtactac
       61 caacaggaat agcatcatat caaagtgcag aaaacataca attattccca aatcctgctc
      121 aatctgaatt aaatattcag caaatagcag cagttgaaaa aatacaagta atagacgtac
      181 ttggaaaaat tgtgtttgaa aagaaaatca aaagccagca aaatttaaag ataaacatca
      241 gtcaatttca aaacggagtt tattttgtaa aggcatacag cgcacatgat gtggtagtca
      301 agaagtttgt taagaggtag gcgatttttg tattgttgat cagccattgc tcagccgcgg
      361 ttgaggggat ttgaatatta ttgtcagaag ttcttcaatt ttaaatcggt ggccacaata
      421 atttttgaag ataagtaaaa taaatttcta aatcagacag cttttattta ttctcttatt
      481 ctgataaaaa tataaaccaa actatttttt atctcatttg aattacatac ttttgatata
      541 accaaaaaag taagtaacat gagaaaactg attatattac tcttgatttg tacaactacc
      601 gcccatgccc aggaaattga atggcaaaat actattggag gaagtggtgt agaattttta
      661 aattacacac aacaaacttc agatggtggc tatattcttg ggggtcgttc caattccggt
      721 gtttccggtg ataaaacaga accttcaaaa ggagattatg attattgggt ggtaaagtta
      781 gacgcaactg gaaatattca atggcaaaac accattggtg gagatgatgc tgattggtta
      841 caatcggttc aacaaactac ggataatgga tatattcttg gaggatggtc taagtcaaac
      901 atttcagcag ataaaacaga aaattcacaa ggggcttctg atttttgggt ggtaaagttg
      961 gattcaatag gtaacattca atggcagaac accattggtg gtgattctac cgataattta
     1021 tattctttac aacaaactac cgatggtggt tacattctag gaggattttc tatatcttct
     1081 atttcaggcg ataaaactga gtattcgata ggtggttctg attattgggt ggtaaagtta
     1141 gatgcatcag gaaacattca gtggcagaat actatagggg gatacgagaa tgataattta
     1201 tattctttac aacaaaccac agatggtgga tatattcttg gcggacgctc tatgtctaat
     1261 gctacaggag ataaaacaga aaattcacaa ggacttgatg attactgggt ggtaaagata
     1321 gatgcaactg gaaatattca atggcaaaac accataggag gaaatagctg ggatgaatta
     1381 gaatctatag aacaaacctc agatggcggc tatatattag gtggttcttc cgattctgac
     1441 aattccggtg ataaaactga aaattcacaa ggagattttg attactgggt ggttaaatta
     1501 gatgcaaccg gtaatttaca atggcaaaac actattgggg gcgataattg ggacaagttg
     1561 aagtctattc aacaaactac tgatggtagt tatatttttg gggggtattc ttcttctaat
     1621 atttccggtg ataaaactga aaactcacaa ggaaactatg attactgggt gataaacata
     1681 gattcaatag gtaatatact ttggcagaat acgattggag gtctttatgc agaagcatta
     1741 agctcaataa aacaaactat tgatggcgga tatattattg gagggcaatc tgtttctcct
     1801 atttcaggcg ataaaacaga agattcacaa ggtaataatg attattgggt tataaaattg
     1861 acagataaat ataatttaat tgatggcaaa atgtatgcgg atgtcaataa caataccata
     1921 caagatgttg gtgaattggg tgtatctaac aaaatggtta ctgaacaaaa tactggaaga
     1981 ttatctttct cacaacagaa tggaaaatat agtttatcgg tgttagatac aggtggtttt
     2041 tctgtttctg catcaacgat aaattattac aatagtgtcc cattagttca tactgcttat
     2101 tttaacaaca ttaatcaaac tgattcatta aacgattttg cattacagcc taatggagtt
     2161 attaacgatg tatgtataaa tcttacacca gtttctttat tccgctcagg ttttgatgca
     2221 acttactata tcagctatat taacaatggt actacaacac ttacgccaac tgtaattttc
     2281 tttcctgatg cgaatgtttc ttttacaagt gctaatgtaa ctcctgtttc tgttacaacc
     2341 gattctgtcg tatggaatat tggcgcactt acaccttttc agtcaggtaa tattgttgtt
     2401 acagtaaatg tacatacggg tttgcccatt ggttcactta tcaattcaag tgtaagaata
     2461 gagcctgttg ccggtgatgc taatcccggt tgtaattaca gttcttggga ggtttttacc
     2521 ataggttcat ttgaccccaa tgatatttta gtaagtagag atactttatt cactacagaa
     2581 attcccaatc caccctattt agattacatc atccgttttc aaaacaccgg aaacgataca
     2641 gcatttaatg ttaaagtttt aaatccatta gatacaaata aacttcagtt aaacacctta
     2701 gaatatgttg catcatcgca tcctgtaaac atgaatttta tttatcatga gcgcaacatg
     2761 gaatttaagt ttgataatat tctattgccc gatagtaatg taaacgagcc gttaagccat
     2821 ggctttattc attacagaat aaaacccaaa acaagtctgg tattgaatga tagcatacaa
     2881 aacaatgctg ccatttattt tgattttaat gaccctgtaa taaccaacac agcaacaaca
     2941 catgttgtac taccaacagg aatagcatca tatcagagtg aagaaaagat actactgtac
     3001 ccaaatcctg cacaatcaga attaaatatt caactcacaa caggagtaga aaaaatacaa
     3061 gtaatagacg tgcttggaaa aattgtgttt gaaaagaaaa ccaacagcca acaatattta
     3121 aagataaaca tcagtcaatt tcaaaacgga gtttattttg taaaagcata cagcacacaa
     3181 ggtgtggtgg taaagaagtt tgttaagagg taagagaata gttgaattac tgatcagcca
     3241 cggctcagct gaggctgagg agatttggtg aactttacag gcaaaatttc agatttgttt
     3301 ttgaaatgca gttgtcgttt aatcttcttc tgattttaag aaattaagat ttctctttat
     3361 ccctttgtat cctgttcgtt gcagcgcaga gtctttaaat atttttttaa aaatttcttc
     3421 tgtaagttct gtccattcat gctttgtcat ttctatcaat tcacttttag gctgcagttc
     3481 ctgatgttga tgtggttttg caaaacggtt ccaaggacat acatcctgac aaatatcaca
     3541 accaaacatc cagtttttaa attggttttg gaaagataca ggaatttcgt tcttcaattc
     3601 aatagtaaga tatgaaatac atttgctgcc atcaacaaca tgaggcatta caattgcctc
     3661 tgtcgggcag gcatcaatac attttgtaca agtgccacag taatctttta ttggagcatc
     3721 ggcatcaagt tcaatatcaa gaatcagttc tgcaataaaa taccaggaac ctgattgcgg
     3781 atttatgaga ttattatttt ttcctatcca tcccaatcca cttttacgcg cccaggctct
     3841 gtctaacaca ggtgcagagt caacaaaaac acgtccgtca attgctccta tatgctgtcg
     3901 cataagttca agtagttgaa acagtttttc tttgattaca aaatgataat ctgttccata
     3961 agcatattta gaaatttgcg gtgcctgtgg attttgcttt ttttcaggat agtaattaaa
     4021 aagtaaggag atgacactcc tagcgccttc aaccaatttt gttgggtcta aacgcttatc
     4081 gaaattgcgt tccatgtacg acatggtgcc atgcatgttt gctgtaagcc attgttcgag
     4141 gcgtggtgct tcttcttcca gaaaaccggc tttggaaatg ccgcaatgtg agaagcccaa
     4201 ctttaatgct tcggctttga cccatgctgt tgtctggcta cggttcattg ttttttaaga
     4261 ctcagagtcg aacaatgttc ccttctgctt taaagacttt ttaccaagat gtttaaaagc
     4321 tttttctgtt gcttcacgtc cgcgtggtgt acgcatcaaa aaaccttcca taattaaaaa
     4381 cggctcatac acttcttcaa tagtacctgc ttcttcgcca acagcagtgg caatagtgtt
     4441 caatcctaca ggtccacctt taaatttttc gataatggca ttaagaatcc tgttgtccat
     4501 ttcgtccaga ccgtcaatgt caacattgag tgcctgaagt ccataacgtg caatttcaat
     4561 atttacggtt ccgttacctt ttatctgtgc aaaatcacga atgcgtctca acagtgcatt
     4621 agcaatacga ggagtaccac ggcttcttcg ggcaatctca aaggccgctt cttcgtcaat
     4681 agcagttttc agaatagatg cagagcgcat tacaatgtct tgtaataatc gtgcatcgta
     4741 atattcaagt cgcgagttga taccaaaccg tgcacgcaat ggcgaagtaa gcaatcctga
     4801 acgtgtagtg gcaccaatga gtgtaaatgg atttatttta agctgaaccg aacgtgcatg
     4861 aggtccgctt tcaatcataa tatcaatttt ataatcttcc attgcagaat aaagatattc
     4921 ttccacaata gggcttaagc ggtgaatctc atcaataaac aacacatcgt tagcttgcag
     4981 attggtgagt aagcctgcaa ggtcgccagg tttgtcaagc acaggacctg aggtaacttt
     5041 tatttttaca ttaagttcgt ttgctataat gttggctaat gttgttttac ctaagcccgg
     5101 agggccatgc agtaaaacat gatcaagagc ttcgccacgc tgtactgctg cttttacaaa
     5161 tacagtaaga ttttctgtta ccttacgttg tcctgcaaaa tcatcgaagc taagcggtcg
     5221 taatgcccgt tctatttcct tttcggctgt gcttaaattt tcacttgccg gatcaagatt
     5281 tttattgata ctcattacca caaaagtaaa atttttggga gacatagaaa gcacataagt
     5341 tacttaaatc ctcaatgcac attgtactat acaatgtgtt ctgtcattct aaagcagata
     5401 tatttattag acatttgaaa caaaatggta aaaagatgaa ggggaaattg tggaggaaac
     5461 agaaaattat tatttttaga caacgtattt ttattggaaa acgtcttgat attaatgttg
     5521 ctatgaagcg tttaattgct gtacatattt ttatgctgct ctgtagtgca ggtcaggcaa
     5581 gtcatattat gggcggtgat tttacctata cactcatagg aagtgtacca cagcaacaga
     5641 aaaaggttta caagataagc ctcaattatt accgtaactg cgatacttct gctgcacaat
     5701 ttcaaccata ttatgatctt tatatttgtt atgattcttc taatgccaac tataaacgag
     5761 cgtatgcaca aatttcgctg gataaaataa gcacacagcc aattgttccg cctatattag
     5821 acactacttg ttattataac aacaattggt gcgtagaaaa aacatactac gaagcgactg
     5881 ttacgctgtc agaagataca acctggcact tgtttttaag tgatggagca agaaatgtta
     5941 gtatcaacaa cctgattcaa ccggatagca caggcatatt tttttataca gttattccgg
     6001 caggcatctc caactctaca ccacagtttt caaattcgcc tacacctttc atctgtaatt
     6061 tagatacgat gcagctaagt aatctggcat ttgacagtga tggagatagt ttggtttata
     6121 aaattgtgca gccttatgga tacataggtt taaacaattt aatgacctat caggatatta
     6181 attttaactt gtattatttg tttcctttac caacagtcat ttacggcccg ggctatagtt
     6241 acactagtcc ttttggtgca ggaggtctct gtagtataaa ttcacaaaca ggattgctga
     6301 ctatctcttc accaaatcag ggattatatg ttttggccat agaagtagaa gagtatagga
     6361 atggaacact tctttccaga acaaggcgcg acatgcaggt agtggttgct gcttgctcct
     6421 ataatacggc accacaatta acctcacctg ttgttttaaa ttatcagata gatgaaggag
     6481 attcaatttg ctttcccctc acatttactg atgcagaaaa caatatgata aacatttcgg
     6541 caacaggtaa tcttttcggt acagcactca ccaatccacc tgcacagttt gttgttaatc
     6601 caactccaaa caataataca gctcagtttt gctggaaaac aatttgcgga caggcagcac
     6661 cattccccta tatgtttaca gtagaatcaa cagataatgg ttgtccttca aaaaccaaca
     6721 cctatgtttt ttctatacgt gtaaagacta ttcctcacaa ccaactgcct tccataaacc
     6781 tgagtatgta tcctgataca cctacctgca ccggtacagc ctttcagttt aatgcctcca
     6841 cacaatttgc cggaaataac cctacttatc aatggtatat taacggacaa gcactatcta
     6901 ataattatcc taactacaca acgaatcaac ttaacaatgg tgatagtgta tcggtgcaac
     6961 tcatcagcaa ttctatttgt gtaattgatt ccagtgcaat ttccaataca caagttgcaa
     7021 cagtgctgca accgacagtt gcttcattca ttacaagcac ggtttcggtt agtagtatca
     7081 atgtgctgca acaatttaca aaccaatcgg tgggtaacac ttataatttc tggaactttg
     7141 gagacagtac acaatctgct gaaataaatc cgcaacatgt ttatgaaaac agtggtgttt
     7201 atttggtaca actcgtaaca gttagtgatg atggttgtcg tgatacaaca gaagcaccgg
     7261 tggatatcaa aacaacaatg agttttttta tgccctcctc atttacacct gacgataatg
     7321 ggttgaatga tttgtttact ccgataggag cagaaatgcc ggattatacg cttcagatat
     7381 ttaaccgttg gggacaatta ctttattctg aaactggaaa accgcaatgg aatggtcgtt
     7441 ataaaaatca actactacct ttaggaatgt acatttactc cattcgtatg cacaatccaa
     7501 cagatcccat tattaaaacc ggaacagtaa tgttgttgcg atgaaaatta tattttattt
     7561 gttggaattc tatcttataa atttttaaat ttccgatatt aattcaccaa ctgtatctac
     7621 tggagaaata agtattttac gtaatgaatt tattttacat gaaaccttta ctacttatac
     7681 ttgcttttta agttctaaaa attctttttt gaacttttta gcataatcat atcctacttt
     7741 gtaaatttca tcaatctttc cggtatcaaa ggtgttaaat ttattgattt catcaggctg
     7801 aagaaaaata tcacatttgt gattttctgc acttttaata tttgaagtgg caatacgaat
     7861 aattcgttgt atgatttctt tataacctaa tttaccttct aatttattaa tcggattaat
     7921 gtgaacacca atactcttat tacatgttgc ggtaatttgt tctactggaa agttattcaa
     7981 caaccctcca tcacataaat atcggtcgtt attatagaca acaggatgaa acaccaaagg
     8041 aaagcaacaa gaagatttaa cagcaaagga taaactaccc tgattaaaaa tcaacaactc
     8101 tgcataagtt aaatctgtaa cggcaacata taaaggcatt ttcaatcttt caaaactatc
     8161 atgtggaata tatttcttaa ttactttttc aaatatatcc gtattgaaca aaccaccatt
     8221 accaatagac aaatcagaga aagtaaagaa tttttcacgc ttagctagtt ctaatatttc
     8281 taaaggggca taaccttcag ccacaaaagc tccaatcaaa gagcctacac tcgtacccga
     8341 tacagcatga ataggaatat ctaactcttt cagatattgc ataatcccaa tatgtgcaat
     8401 cccacggaat cctccacctg agaaagtaat tccaatttta ttgtttacaa tcataatggt
     8461 taactcataa aatttatagc aaatataaag ctatccgatc aaagtaataa aatgtcaaag
     8521 tatgtaataa gatgcattca ttagggaatc tgtttagtgt acttgcttta caatattata
     8581 tttgtaaaaa caaaaattct tgaaacaaaa acaaattagt atgcagagta tttacgaaag
     8641 taaattttac aagtatctgt tttatattaa tcagcggaca caactataga atttaaaatg
     8701 tcattttgtt attatgaaat ggatatttat tacagaaatt atttatgtac ttatagttat
     8761 actggtatgc ttacgaatta tttgggatac cgggaccact actaaagctc tggcatatct
     8821 tttattggta atctttgtgc cattcattgg aatattcttt tatttttctt ttggtattaa
     8881 ttataggaaa aggaagatgt acagtaaaaa aatatttcaa gacgatgtgt tggctgaaag
     8941 gttcagggag caaattcttc aatattcaga aaaaacatat agagagaata tagatatagt
     9001 tgagaacaac aaagaacttg cccgacttct gacctatgat agtttaagtg ctttaacttc
     9061 aggcaatgaa gtaaaattat tgattaatgg agagcaaaaa tttcccgaag tccttaatgc
     9121 aatagcatca gcaaaacacc atattcatat tgaatattac attttcgaaa acgatagtat
     9181 tggtaagagt gttatagaag ctttaatagc taaagttggc gaaggaataa cggtgaggtt
     9241 tatttatgat gactttggaa gtcatgcatt aaataggaaa atactgaaac ggatgaaaga
     9301 tggcggaatc aatctttttc ctttttcaag agtaagatta tttgcattag ccaatcgcct
     9361 taactacaga aatcatcgaa aaattattgt aatagatggt aatattggat ttgttggcgg
     9421 aattaatatt agtgaacgtt atataaattc agaagagcaa ggtaagaaat tgtattggcg
     9481 cgacacacat ctgagaatag aaggtgcagg cacatactat ctgcaatatc tttttttatg
     9541 tgattggaat ttctgtgcac agtcacaatt gcaaccttca agagaatttt ttccatcacc
     9601 aactttattg aataacaaag ggaataaaat tgttcagatt gcagcaagtg gaccggattc
     9661 tgagcgaccc acaattttat tttcattact acaggctatc agtctggcct caaaagaaat
     9721 attaattaca acaccttatt ttattccggg agaaagtatt ttggatgctt tgatggtagc
     9781 tgctcttggt ggagtgagtg ttaaaattct tgttcccggt atttctgatt caagaatagt
     9841 aaatgcagct gcattttcat attacgatag tcttttaaat gcaggtgcag aaatttattt
     9901 gtaccagaaa ggctttattc actctaaaac attggttgct gatgataaaa ttgcaatggt
     9961 tggaacagca aatatggatc atcgtagttt tgacctcaat tttgaagtca atgctattgt
    10021 atatgacgaa acatttgcga aagaactaaa acgtgaattt tataaagatt taaaagatgc
    10081 tgaaagtata gatgtgaaag cgtggaacaa ccgccctatt attaaacaat tgggagaaaa
    10141 atgcgcacgg ttaatttctc caatgttata atcattaaaa agcaaaggca ctataaagtt
    10201 tttaaccctt ttggagataa aataccaaac agaaacattt tatttagttc ttcaccccaa
    10261 tgtaaagatt tgctaagcat ttgttgtttc attaaactcc atagttgcag gaagtgcaac
    10321 tgtacgacaa catcgggctc aatgtcggaa cgataatatc cttgcgacat acctttttta
    10381 atattattta taaaagtctg ttgaatattt tttgtgaaat ttagtatcag ttgatatgct
    10441 tccggtttta ttgtttttag tgaatcaata aagggaagag aaatttcttg cgaagcattt
    10501 attgtaaata aaagaaggtt tgtcagctcc tgtacaacgt ctttgttatt ttgcaagatt
    10561 gatttaatct cgttttcaaa tatatgaacc tgttgttgtg taacagtttt taccaaacct
    10621 tctttatcaa aacatttata tagtgttttt ttggaaatgc cacatgcatt tgcaatatca
    10681 tccatactta ctgactttat atctaccttc atgaacaaat gacgcgctct attgacaatt
    10741 atattttcaa ctgctttatc gtttgaatta aatggagtgc attcagcgtt catgaatgtt
    10801 tatgcttaaa ttacatgaga tattgaatgg ttatctgctg tatttgtatt ttcagcatgc
    10861 atgagttcgc gtactatttt tttcttattc aaatcgcccc aaccaaaacg agcagaaacg
    10921 ctatccataa ttttataaat aaccggaaca actattaatg tcaggaacat ggatgaaatt
    10981 aaccctccga taattaccca ggccaaacca tgtttgatag cggctacacc acctgatgcc
    11041 aatgcaatag gaagcatacc aattaccata gcaatggttg tcattaaaat tgggcggaga
    11101 cgtgcattgt tggcatgtac caaagcttcg tctgtagatt ctcctttttc tttcatttgg
    11161 ttagcaaagt caaccagcat gatggcgttt tttgccacta aaccaatcaa catgataact
    11221 cccagaatac tgaaaatatt gagtgtgtta ttagtgagtg ctaaagcgag caatgcacca
    11281 atcagagaaa gcggaataga gaacaataca acaaacggac gcacataatc atcatacaaa
    11341 gcaaccataa tcagatacat gataataata gaaagtaata atgccaaacc taatgtacca
    11401 aatgcatttc cctgattttc ggcatcgcca ccaaagatgt aattgacacc ggcaggctta
    11461 tccaattttt tcagactttc ggtaaaatca gtggttacag aaccagcagg tctgcctatt
    11521 acttgtgcct gtacatttac tgatgcaagc ttatctctgc gctccaatac agtaggtccc
    11581 gaagtttcag atatagtagc aaattctgac aatcgaatgt tctgaccttt attgtttaca
    11641 aacataatgt tgcgcacatc atcagcattt tttcggttga agttatcgta gcgcaggtta
    11701 atatcatatt cgtattctcc ttcgcggaat ttaagtttac tatcctgatc ggtaccacta
    11761 aaagctgttt gcattgtgcc gcctacaaca tccatattca cacccagtag agccatctta
    11821 tcacgatcaa gtgttactcg cacctcgggg ttaccatctt ctgcagataa ttttgggtca
    11881 gacacaccat caattttttg taatgcaagt aatgcttttt tagcaaaagc catagcgcta
    11941 tcttgctcgg ttccggtaac aaccaaatca atagaagcac gttcggcagt acccatgatg
    12001 cttacgggag aagttgttat tttagctccg gctaattgtg cagctaattc ctttttgatt
    12061 tttgcagcat acatgcttga aggctcgctt cgcagatttt catcaatcat agtcactgta
    12121 atttctgatt tgtaaggtgt agatgatgaa gtgccaaatc ctccatcgct gctttttcct
    12181 actgttgtgg ttaatgactt taccaaatcc tgtttagcta agtaattttc agcacgcata
    12241 gtcatttgat tggttacttc caaagcaaca tctttaggca tttcaatggt tacaataaac
    12301 tgtccgcgat ctcctttagg tataaactct ccaccaataa acccttttgc aagcaacata
    12361 aaagatgcaa ccagcaatac aattgtacca ataagtacat agcgcttatg accaagcgac
    12421 caacgtaata tgccggtaat ccattctgta aacttatcta tttgacgttc gaaccacaga
    12481 ataaattttt ggaacatgtt tttacctgtc agatgttcga gttttccaaa gcgtgatgac
    12541 aaccaaggaa caacagtaaa cgacaccaac aaacttaaca gtgttgaaat tactactgtt
    12601 atacaaaatt gacgcaatat cattgacact aaactattgg ttagtgatat tggaagaaat
    12661 actactacaa ttactaatgt gatggaggta acagtaagac caatttcttt tacaccatca
    12721 aaagcagcac gaactttatt tttacccatt tccatatggc ggtaaatatt ctccaacacc
    12781 acaattgcat catcaacaag aatacccacc accaaggaaa gggcaagcaa agacatgagg
    12841 ttaagtgaat aacccattac atacataccg gcaaatgtta ttatcaatga cagcggaatg
    12901 gaaatcatta caatcagcga gtttcgtatg ctgtgcaaga acaacaacat tacaaccgct
    12961 accagaataa tggcaataaa taaatcgaat acaacatcgt tggccgattc cagagtgaac
    13021 tctgatgaat cagatgctac gaacattttt aaattgttac ttgcatattc tttctcaatt
    13081 ccggcaattt gattgtgtac ttctttacta acggttacag cattagcatc agattgtttg
    13141 gtaatttgta ctacgattgt ttctttctga tcctgacgtg ccaatttatt tacatctttt
    13201 tgtccatcgc gtacatctgc aacatctttc aagcgtactt gcgaaccatc atcactagtt
    13261 ttaattacca gattgcgcaa catatccaca tcggcatatt ttccggaaag gcgtatggct
    13321 atcagattgt cggtggtttg aattttgcct gtgggataat caacgttggc attggtaata
    13381 gtttgtcgta attgtaacaa cgatagtttt tgtgcattaa gtttaccggc atctacattg
    13441 atttgaaatt cacgctcagt gccaccaatc atttgtactt gtgctacacc cggtagtcgt
    13501 gaaagacgag gtgccagccg ttgatctaaa agatcaaaga attcacgcga atccatatcg
    13561 gcagttactg acaatgtgat aataggtaga tcatcaacag aaactttact gagtgttggt
    13621 gttttagcat cacttggcaa atccgacaac attgcattta ttttacgttg tgcatcttcc
    13681 aaacttctgt tggcatctgc tcctacattc atttcaactg taactataga aactccttcc
    13741 atagagcgtg atgtaagttt ctttatgttc tccatagaag atactgcatc ttcaattttc
    13801 tttgttacag tattctgaac ctcatcagga ccggcacccg gatataccgt gctgatggtt
    13861 accattgaag tactaaattt aggcaataac tcataattga gagagctata actgataaga
    13921 cccagtatgc ctaatattgt aaaaacgacc acaaccattg taggtctgtt tattgatatc
    13981 tctgtaattt tcatttattc gatttttaaa tgttagcttt taaaacagaa cttattttac
    14041 aatagaaatt tgtttgccat ctaccagatt aatttgtcct gtaacaatta cctgatctcc
    14101 ttcttttagt ccttcacgaa tttcaaccat atttccgtga acatttccgg caactacttt
    14161 ttgcaatttt gctttgccat tatcattaat aaaaattaag ttactgttaa taccaccttg
    14221 aaatgcagag cgcggtatca ttaaagatgc attttttatt tcaggcttaa aatgtgctgt
    14281 accatacatg ccggctttta gtgctttgcc tgctatgtta ttaatttcta cctcaacagg
    14341 gtaattcaat gaagcatcac ttttagcagc aatgaaagtg attacacctt gatatgttac
    14401 ttcaggatat accgatgcag ttacttctac tttatcacca gtgtgcaatt gaataatctg
    14461 atattcattt acattaacag tgagttttaa ttttgcatca tcaacaattt caacaatctt
    14521 attgccggaa gccagataag ttccaacctc tacataacgg gtattaataa ttccatttac
    14581 aggagctttc actacagagt tataaagatt tttatttgcc tgaacatttt ttgcttgtgc
    14641 actttctaat tccaaacggg catcatcaac ttgtttctgt gttacgcctc ctgttttata
    14701 tgcttgctca taacgattta agtttaatct caactgctcc aaccgtgctt ttgcttctgt
    14761 ttgatttgca ttcagaattt cattttcaat ttttgcaata gcctgtccgg ctttcactac
    14821 acttccttct tttacatata actcactaac cctacctgca atttctgctg ttaactcaat
    14881 ttgatttacg gcttcaaaat ttccgttctc gtcaaatgac aaatcaacgt tagatgattt
    14941 aactgctaaa atttgtacag gaacggctcc acttgagctt tcacgcacca cttcaatgcg
    15001 tgctgcatct gattttttgt ttgcatttag tttccaaaca ataagggcaa gtgccactat
    15061 taatgtacct ccgtatataa ataccttttt cataatttat tattctttat tttaattgac
    15121 tttagttgat aagattgaga attgtaccgt ttgaacgcat aagctcaagc tcggcaagtt
    15181 tatagtttat tatgcttttt gtataaatat ttttagcttc cagaaaagaa gtttgtgaat
    15241 cgagtaggtc tgttagtgaa cttaaaccta aagtatagtt gcttctgtta gattcataaa
    15301 cagtttcaga tagtttcaca ttttcttgtt gtgcacgagc agttgtcaat gtattttcca
    15361 tttgaatgcg tgcactttca tttttagctt caagagttat tgctgttgcc tgtctttgtt
    15421 tctcgagttg atgaatttta atattgttct gtgcaacttt tgaagaacga ctaaaaccgc
    15481 tgaataatgg tattgataaa cgcactccaa cactacccat accatagcta atggcagggt
    15541 cagttccttt atataagtca gaaaatttat cgctggccat attatagttg tagtttgcaa
    15601 aggcatttag ttttggatag ttggctgcac gtgttgcatt acgttcaaga gtactcaatt
    15661 ttatttgcgt gtctaatagc ttcagatctg tgctggcatc aggattatat tgttgttgtt
    15721 gcaatgcaac ctgtagcgat gtttcatcta ttttcatact atctaatagt atagaggtgt
    15781 tgtatggcag acctaaagtt accttaagtt gatttgtttt tacatcaaca tcgttttgtg
    15841 cttgcgttaa ttttgattgc gcattgatga gatttacttt tagccgatca acatctactt
    15901 tacgaagtaa accattatta tactttcctt cggatgcctg aacaagttgt tgcataattt
    15961 caattgtata atgttgtagt ttaacctcct ccatgctact aagaatctga taatactgct
    16021 gagcaatatc attgacaact tgttcctgcg tttgttgagt atttacacgt gcataaaccc
    16081 ggccggcttt ggctgccttt aaaccctgaa gtgctgctac gtcaaaaagt gcttgtgtag
    16141 cctccacacc taaggcagag ctataatttg ttccggcttc aatttttctg gatccttcac
    16201 ctgtaccacc acctaatcct gccggtagta caagaacttg tcttttgtag ttgtttgtta
    16261 tattacctgt tccgtttacc tgaggaagtg tcaggccacg agtttctact atttgtttct
    16321 caacaatggc ttcatccaat ttgcttaatt gtatattata gttctgctct aatccaattt
    16381 gtattaaatc ttttaaattt aatgatttgg tttgtgccat aacttgtgtt gaaaaaagca
    16441 taattgctat tgcaagtgca ttgtttttaa tacttagctt catgtttatt tgatttttgc
    16501 aatttatttg agtgtctttg aatacggggt caaaagtgca tgtattttaa tgtatatagg
    16561 tatctaaatt gacttaaacc gctaaaatag ccgctgaaac gacttagtag gaaactgaaa
    16621 tttttatgct taaaaacctg ttaaataatg gatggataaa cttgaaatcg tttcggtaag
    16681 gtaattggca gtttcagttt tatatttagc atgttgggtc ggtaaaggca atcaatattc
    16741 gcggtaattt atttgttttg ctgaacaatt acgcattctt ctaatgaata agatagaaca
    16801 tagaaaatga ttacctcaaa aacaaaatgg attgaattta ttaataagta ctataaatac
    16861 tttttaattc caattgtttt ttttacccat aaattattgt tttttttaac cgatccttat
    16921 gcacaaccta ttcgtaaact tacatttcag gaaagtttta ttcaaaattt agggctgcta
    16981 tttttttcat tcattctggt ttatggtagt gttagaaatg ttaacagact aaacaagcga
    17041 ttaccatggg ctgcatcacc aatacttcgt ttaatagctc aagtttttgt gcaactcatg
    17101 ttgtcaacaa tatggatatt tatttatcag tttgttgttg ttaagatgtt atttgatgta
    17161 aatatttttt ctctcataac atcacctgat cttacaccgg gaatacggtt tgttatctgg
    17221 cgcttctttt tcacatcagc attggtatca ttgtttgtaa gcctgattat tacaggtcgt
    17281 aattttcgaa tggaaactgc tgagatgaaa attcaggcag cgcagcttaa acaaatagca
    17341 gcacaagccg aattacaggc attaaaactt caacttgacc cacattttgt gtttaataat
    17401 ttcagcacag tatcagcttt aattgaagaa gataaagcac tggcacaatc ttttgtcgaa
    17461 aatctgtcac gtgtgtatcg ttatatggtt caaaacatac atagcgatac aattactttg
    17521 caaagggaag ttaagtttat agaatcctat atttatttaa tcagcatccg tcatggtgac
    17581 aatgtgcgta taaaagttaa tatccccgaa gaatttcttc agaaaagaat tccgcctata
    17641 actttgcaac tcctcatcga aaatgcaatc aaacacaata ttgcttcaca tacaaaacca
    17701 ttgaacataa atatcggtgt agcagatgat atgttacgcg taagtaataa tttacaaaga
    17761 ttttcaaatc cctcacaaat ttcaacacgt attggtattg aaaatatcaa atcacgctat
    17821 agaattgtgt caggaaaaga accggttgtt attgaaactg ccgatgattt tatagttgaa
    17881 ctcccattat tggattaaaa tagttgtatc atgaaatttt taattgttga agacgaaaaa
    17941 ccaaatgcta accgactgaa gaaaattgtt gagcagttgg atagctcatt tcaggttgta
    18001 gatattattg actccgtagc aggagcagtg gactggttta ataaaaatcc acatccggat
    18061 attgtattaa tggatattag attggcggat ggattgagtt ttgatatttt cagtcaggta
    18121 aagattagca gtgcagtaat ttttactact gcttatgatg aatatgcttt gcaggctttt
    18181 aaagtaaatg gggttgatta tttactaaag ccaattgaaa ttgaagaatt ggatgctgca
    18241 atatcaaaag taaagcagaa tcatatcagc gcatcaaata atccttatct tgatgaatta
    18301 attggatata taaaacaaaa agaagtgcag tacagaagcc gatttttgtt tccatataaa
    18361 gatggctata aaacaattaa tgtatctgat atagatttga tttattctga agacaagaat
    18421 actcatttaa ttttacaaaa tggatctgta caaatagcta atcagacttt agaggagatg
    18481 gaagagcaat tagatcctgc tgtttttttt cgggcaaaca ggcaatacat tattaatata
    18541 aacagtattg aaagtatcca caatcacttt aatggcaaac taaaagttgt tgtgagaaaa
    18601 cttccacaac acgatatatt aatcagtaaa gaaaaagcac ctttatttaa acagtggctc
    18661 gatagctgag caactttatt gctgaaaaac tcgtaaattt ttctcttagc ggccttagcg
    18721 tgtttttttt ctttgtgttt tagcgagaga ctttttttat ttcacgcaaa gtacgcaaag
    18781 gagtatttgt agcaagtgta gttttctttt cacgcaaagc tcgcaaaggt gtttttttct
    18841 tagtggcctt agcgtgtttt atttctttgc gtcttagcga gagacttttt ttatttcacg
    18901 caaattaggc aaagttgttt ttcttagtgg ccttagcgtg ttttatttct ttgtgccttg
    18961 gcgagaaact tttttttcat gcaaagaacg caaaggagta tttgtagcaa gtgtagtttt
    19021 cttttcacgc aaagctcgca aaggtgtttt tttcttagtg gccttagcgt gttttatttc
    19081 tttgcgtctt agcgagagac tttttttatt tcacgcaaag taggcaaagt tgttttcctt
    19141 agcggtctta gcgtgtttta tttctttgcg tcttggcgag agactttttt tatttcacgt
    19201 aaagtacgca aaggggtatt tgtagcaagt gtagtttttt tttcacgcaa agatcgcaaa
    19261 gggcgaattc tcttagcgtg ttcagtttct ttatgtctta gcgagagaat ttcttttaat
    19321 gcaaagaata ccttagctac tcatattata ctaccttttt gagttaacag tagcatttta
    19381 ataagttgtc aataaacatc tatgaggcat aacaccactc tttatttttg taagacaaaa
    19441 gaaaattatg ttgaaacgat ttgtttgtat aacaatatca ctattggctt ttattattgt
    19501 gaacgcacag gataataaga ctgaaaagaa agacagtatt gaacatcgta tattactcat
    19561 tccctacgac ccacgatttt atttgtcaga tgctgataaa gacattgcag aagaaacccg
    19621 aaaagaccct aaaacaatcc gtaattattt tcacaatcgc agcgaatggt ttacctcgcg
    19681 cgaactaaaa agacattatc ctactatttc acttttacaa aacgatacgg tgcctgccta
    19741 tattcagtca gcaggtgata tattcagcca aacagcattt gactatgcta cacctgtgac
    19801 acgatcaccc gaaagtatga tgaatgcaac atcagataaa acactgaccg attcgcgtac
    19861 agcaaccaat tatctgaata cagatgtaca caaccgctac atgaaagccg atattcagaa
    19921 taaaaaagta ttagccgatt tgagcgagcg atttaataca gatatttttg tttttcttac
    19981 tcaatttgag attaaaacca attataaaaa ctgcttagat attgccaata aaatatatga
    20041 gcgagaaatc agacttcatt ttaccatcta tgataaaacc ggaaagctaa tcaatggaaa
    20101 ctatgccatt gcagttattc catcaaacgt aaatcagatg gacgaaatta ttttaaagtg
    20161 ttttcctctg ttggcaaagg ggattgcagc atcattatga ggtaaaaatt taattatttg
    20221 ttacccggat gctatctttt cctaccttta gtttacattc gcaacttcaa tttattcaaa
    20281 catgagaaaa cttttaacac taagcgcatt atttgtaatt ctgtcaggga cacaatcgtt
    20341 ggcaagtagt ttttggactg ctaagtcaga aactcaagaa atgttatcag gcaaacgtca
    20401 gattgttcct gataaataca aggtttatac tcttgatgta caagggctga aaaaccaact
    20461 gctcaatgcg ccaatggaat ttaccatggc agcaaaacaa tcaccggttg tgattacatt
    20521 acctatgccc gatggtagtt ggaatagttt ttctatggta gagtcgcctg ttatgcaacc
    20581 caaactggca gctaaatatc catttattaa aacatactca ggcagcggta taaacaatca
    20641 accttcatgg gttcgtatag attttacaca atggggattt catgcattga taagaacagc
    20701 ggagaacgat atttatattg acccttattc catgcaaact acctctgatt atttagttta
    20761 cttcagaaaa gattttacaa agtcgcagag cagtatggtt tgtgagtttg acgacttaca
    20821 acaaggtcaa agtgcaaata agaccttaac aaactcaatg ccttcagtga atcgttctat
    20881 aggtacaaat ctgcgtacct atcgtttggc attagcctgc accggtgagt atgctgcata
    20941 ctatggtgga acaaaagcag gagcgttgtc agggatggtt acatctgtaa atcgtgtgac
    21001 aggtgtttat gaatcggaag tagatgtaag acttaattta attgctaaca ctgatacttt
    21061 aatttttctt aatgccaaca ccgaccctta taccaataac agtggcggaa ccatgctggg
    21121 agaaaatcaa tcagtcgtaa acttttttat tggtactccc aattacgata ttggtcacgt
    21181 ttttagtacc ggtggtggag gtattgccta cttaggttgt atctgtaaaa ctgcagataa
    21241 ggcaaaaggt gtaactggat ctgctgcacc aatcaatgac ccgtttgata ttgattatgt
    21301 agctcatgaa atggggcatc agtttggagg caatcacact tttaacagcg taacaggaag
    21361 ttgtcagggt aaccgcgaag ccggtgcagc ttatgagccc ggtagtggtg taaccattat
    21421 gggttatgca ggaatttgtg gctctgatga tttggctgca cacagtatag cttattttca
    21481 tacaaaaagt tttgatgaaa tagttgatta ttcaaccttg tcaactggca actcttgtcc
    21541 tgtaaatacc atcacgttta acaatgcacc tactgttagt ggcggaggca tatttaatgt
    21601 tcctgtaggc acagcattta aattaaatgg ctatggcagc gaccctgatg gtgattctat
    21661 aactttcagt tgggagcagt ttgacttagg ccctgcggga gcatggagtt tgccaacagg
    21721 taatgcacct atgtatcgca gttatgctcc ttcaacttct gcacttcgtc tttttccaaa
    21781 attgtctgta ttgctcaatc aggtaaatgc taaaggtgag ttgataacat cttatgcacg
    21841 ctcattacat ttcagactta ctgcaagaga taacaaaccc aatggtggtg gcgtaactta
    21901 taacgataca gttacaacag taaatgttgt tcagacttct gcaccttttg caattactta
    21961 tccaaatgta acgggtatat catggcccgc attaagtaca caaacaatta catgggaagt
    22021 gggtggcacc gatgctgcac catttaacga acagtttgta aacatttacc tttcgataaa
    22081 taacggaaca acattcccca taacaattgc aactaatgtt gctaacaatg gcagctatac
    22141 ttttactgtt ccaaataacc catcaaatat ttgcagaatg tgggttgaag gtgccagcac
    22201 aggttctata ttttttgata ttaacaacaa agtatttacc attacagcac ctgttggaat
    22261 taatgaagtc aatagcctta gtcagcttac tacctatcca aatccagcag acaatgagct
    22321 gttggtttat ttaggcaaca attatcatgg tcagctaaac ataagtcttt atagtgttaa
    22381 cggacagctt gtgttgtcac aaactatagc agctaaaaat gctacagcac aggtagcttt
    22441 gaatgtagag caactccccg caggtttata ttttctgaaa gcagaaacag aaaacgggac
    22501 agcagagcgt aaagtgatga taaaataata tcccgataat tcatgaaagg gttgttgaat
    22561 aaacagccct ttttttatgt tttaattttc ggtttaaata aagttcaaat taactacttt
    22621 cgcatatcac ataaaacaaa aaaggtgaag ccatttttca aagccgtatt cacgctctta
    22681 tttactttta taattctaca ttctgctgat gcacagtttt atcagggctc gcagatgtcg
    22741 tttggcaaaa acagagtaca atacacagat ttttactgga catttttcag atttaaaaat
    22801 tacgatgttt ataattacgt aggcggaaaa gaattagcca cctatgttgc tgaacaagca
    22861 cagaatgaaa tggatgttat ggaacgcact ttggattatc ggagttcgtc acgatttcag
    22921 attgtaatat ttaataaact cagtgacctc aaacaaacca atattggcct tgaattagat
    22981 gatcaggctt acaatattgg aggaatgaca aaagtaatag gcaacaaagt gcttatttat
    23041 tttgatggca atcatcagca tttacaagaa cagattcgtg ccggcatggc aagagcctta
    23101 cttgaacaat tgttttatgg aggaaacatc agagaacgat tacaagcttc tattcttctt
    23161 aatcttccac agtggtacac acaaggcttg gtaacctatc tttcaaaagg atggacacct
    23221 gaagatgata acaagctgcg tgattacatc atgacagaaa aaaaaccggt cttaaataaa
    23281 ctcattgagc gtgatgaagt ttttgcaggt cagtcgcttt ggcattacat tattacaact
    23341 tatggagaaa cttcattaag taatttggtt tatatgacac gcattaaccg cagtctcgaa
    23401 agcggttttg tttttgtgct tggtgtacgt ttaaaacaac tttctgccga ctggttaagt
    23461 tatcacacaa aaaaatatca ggagtttgaa aagaataaat ttccaattgc agcaacacca
    23521 ttaaaagtta aagaaaagaa taaaataatt tatcaggtaa agacagatcc ttttggaaac
    23581 agagtaagtt atgttactaa caaacgaggc cgctataaag ttaaaattac ggatgtaaaa
    23641 accggtaaac gtacttgtgc tgataaaggc ggatatgttt cacttactac ggaaaacgac
    23701 cgatcatttc ccattacagc atggcatccg tcaggtaaaa tattagctat cgttaaagag
    23761 caaaaaggaa atattattct gaatttgttt aacacagaaa caaagaagtc tgacttttca
    23821 gagataatga gcttttcaaa aattctcaac atgacctatt ctcctgatgg gtcaaaacta
    23881 ttgatgtctg cagtgcagaa agggcaaagc gatatttatg tatttaatgt gcgcggacga
    23941 aatttcgatc agattacaaa cgatgtgtat gatgacttat atccacaata tgatattaca
    24001 ggcaacaaaa taattttttc ttcaaacaga agcaatgata ccttaggtgt tgatgttaag
    24061 caaaatacgc cacagaacaa ttttgatctt tttatggcag acctctacaa tagagaagga
    24121 aatttattgc gcataaccca ttcaccggag aatgaaacct tccctgttat gagtgattcg
    24181 cttcggattt cttatctgtc agataaaaac ggtgtcatca atagatttgt atcggagatg
    24241 gatagcacca taagttttat tgatacaaca gagcattaca gatatttttt cagacattat
    24301 gctttaacca atagcaacag aaatattctt gaacaggatt tttcggcaag aaaaaataag
    24361 tttacagaac tagcagtgaa caatggccgt tacagaattt acttgaaaga caatatgcct
    24421 tttgataaat caaagacatt tgatttgagt tatatagcac aacctgtttt tcaaccgaaa
    24481 caagagaata aaccaaaggt tgctgaaaca cccactcgtg ttgttactat tgagccatcg
    24541 cagaagaaag acagcgctgc tatagatgta ggcaattatg tttttcaaag tgaattcaaa
    24601 tcaaagaaga agaaagaaga accaatagaa aataaatctg ttgctacaga tactaccaaa
    24661 attgtgcagg aacaagtaaa gttgcaacct gatagtaatg atgtaaaatc tactcccgac
    24721 tctgcaattt tccgaatgcc aaagcaacgt aactacgata tttcattttc atcagactat
    24781 attctcacac agctcgacaa cagtttattc aacactacct atcagcctta cacaggtggt
    24841 gctgtttatt ttgatccggg tatgaatgca ttgttcaaaa tcggtatgag cgatttgatg
    24901 aacgattaca aaatcagtgg agcgtttcgc attggcttcg atttcaacag caatgaatat
    24961 atgctgatga ttgaatcttt gaaaaaaaga attgataagc agatactttt ttcacgtcaa
    25021 tctaaattat tttctgtttc agaccttgat gtgttacgta cacataccta taatttaaaa
    25081 tattcaggta agtatccttt cagcgaaatt tctgcagtta aaggaacgat tgatgcacga
    25141 atggataaga ctatttatgt tgcgacagag aaggcacaat tagagaaaag cgatataaca
    25201 aattattggg caagcattaa aggagaatat atttttgata acactatcaa taaagcattg
    25261 aatctttata atggtacacg attcaaaatt tttgctgaag tgtttcgtaa aatcaataag
    25321 cccgaaaaaa tggtttacat cattggtgca gattttcgtc actatattaa agtacacaga
    25381 caaattatct gggccaaccg tttatcggca agtacatcat taggtaagca aaaattaatt
    25441 tattatttgg gttcacagga taatgttatt gttccgggaa ataattttaa taatgaaatt
    25501 agtgttgaca aaaacgaaga atatgttttt cagacattag cttcgcctat gcgtggtttt
    25561 aaacagaata tccgaaatgg tagcagtttt gcattgttca attctgaatt gcgtattccg
    25621 gtatttcaat atttgctcaa cagaccaatt cgttctgatt tgattcgtaa ttttcaggca
    25681 gtagtatttt tcgatgcggg tactgcatgg tcgggcacat caccattttc tgataagaac
    25741 ggattgaatg aagaagtaat tgggggcgga ggcagcaatc ccgtaaccgt tactgtgatt
    25801 aaacaaattc aacctgtagt tggtgcttat ggctttggat ttcgtagtag aatattcggt
    25861 tattttattc gtgccgactg gggttttgga attgacgatc gtcaggtgca gaaaccacta
    25921 tttaatcttg cacttggact cgatttctga ttatcgcaaa acaaatgtac gatagctgtc
    25981 taattttatc agaataaata agaggattgt aaatccccac agtgatgagc caccgtaact
    26041 aaaaaagggt agaggtattc ctattaccgg catcaggccg attgtcattc caacatttac
    26101 cataaagtga aagaatatta ctcctgccac accatagcca tagattcttg caaaggccga
    26161 cttctgccgt tctgcaataa aaataattct atataacaat gccagaaaca atagtataac
    26221 agttgttgta ccaagaaacc cccattcttc gccaacggta caaaaaataa aatcagtact
    26281 ttgttcaggg acaaaattgt atttggtttg tgtgcctttt aagaaacctt tgcctgttaa
    26341 accacctgaa ccaatagcaa ttttactttg atttacattg taacctgcac ctttaaggtc
    26401 ttcttccatt cctaaaaata cctgaatacg ttttttctga tggggttcta aaacatgatt
    26461 aaacacatga tcaacactta ccacaacacc aatacaaata gcaaccaatc ctgcaacaac
    26521 aaaaaatgtt ttccatttac gaaatagcaa aacaattagt gcagcaagta ccaacaaaga
    26581 ggtgacgagt atcatttttg gtactaacaa tgaaaccaca aacaatacag cagcagaaat
    26641 accaatcatc agcaaccatc cgggaataat ttcttcgcga taaagaacaa tacaaaatga
    26701 cagaaacacc aatgccacac cggtttcatt ttctaataga atgagcagaa gtggaataaa
    26761 aaaaattgca actgcataaa tacgcgttcg ccaggtgttt ttctttaacc ccataggact
    26821 taaaaatttt gcaatcatca tggccgtagc aaattttgca aactcagcag gttgaattcc
    26881 aaagtcacca aaacgaaacc acgacttact acctgcaaca gtatgaccga aaaataatac
    26941 agcaatcata gagattatac ttattgcata aatgacaccc gcaaatgcat cataaaactt
    27001 tccatcaata atcagaataa aaaatgctat aacaagtgat gtaagtatcc acatcatttg
    27061 tttgccatag ttttgcgaaa agtcagttat tacactatgt tcttcattat aaactgcagc
    27121 aaaaatgttt agccaaccgg caataaccat aactaaatat agcagtacca gcaaccagtc
    27181 aatattctga aaaacacttt tctgcttcct cattttttag tcatctaaaa cagttgaatc
    27241 atttgccatt ggaactatca ctgcatctaa cattcgtttt tcaagatcag gacgttttat
    27301 tgtcctcagt aaatatttct gtaccatcaa acttgcaata ggtgcagcaa agtgtgcacc
    27361 ctggccggca ttttcaacta aaacggcaac tgcaattttt ggattttcgc gtggagcaaa
    27421 acaaaaaaac acagagtggt tttttccatg cggattttgt gcagttcctg ttttgccaca
    27481 catcaccaaa ctgtcaacac gagaaccacg ggcagtgcca cgctcaaaaa cccattccat
    27541 accatcttgt atggcatcaa aatattttgg atcaaccaac gtatagtttt tctttttaaa
    27601 aagttcgggc aggtaatgct tatatccgat ttcttttaca atatgaggca cataataata
    27661 tcctttattg gctacaatac aagccgcatt agccatctgt aaaggggtaa ttcctaattc
    27721 accttgtcct attgctaatg aaattattgt tgatgatttc catcgtcctt ttccaaagaa
    27781 acgattgtag taatcaactg ttggaacgtt gcctcgcaat tcatgcggca tatctacatc
    27841 aattttttta ccaataccaa agcttagaac ataatttcgc catgcatcaa aagcatcttc
    27901 tgttctgttg tattttctat tgtcaatcac ccctcggaaa gtctggcagt aataagagtt
    27961 gcacgatact gcaactgcag tacgtaaatc aagaggagag ctgtgcggat gacaatgaac
    28021 aaaaatattt ccaaccctat aaccacctgc acaactgaaa tatgtttcag gtgtgatgac
    28081 cttctcttgc aaaccaatga gtgagttgat aattttaaat gttgaaccgg gaggataata
    28141 ggccatcatg gcacgattaa aaagtggctt atcctcatct tttaataaac gaccatagtt
    28201 gcgcgaacgc tctctaccta ccatcaggtt aggattgtag gtagggctgt ttaccatagc
    28261 aagtatttca cctgttgaag ggtcaatggc aacggcactt cctcttttat tctgaaacaa
    28321 tagctctgcg tactcttgta attctgcatc taaggaaagt tttaaatttt caccggatat
    28381 ggcaagtgta tcatatttcc cttctttaaa acgtcctttc tcacgattaa aattatctac
    28441 catcatatat cgtgtaccac gtctgcctct taattctttt tcataactct gctcaatacc
    28501 actgatgcct atataatcac ccatctggta atagttattt tttgcagtca cattttcatc
    28561 tacctcacca agataaccca gcaagtgtgc tgcagtgttg taaggatatt ttctgatggt
    28621 acgcggttga acactgaagc ctttatactt aaaaagtttt tcctgaagtg ttccgtatgt
    28681 ttctgctgaa atctgttttt caaaaattga tgaacgataa ggagaatagt tttttgcttt
    28741 ctgaaatttc tttctgaaat cttcaatact gatttttatg atggaacaaa attctgctgt
    28801 gtccatttct ttgacttcac ggggtgttat cattaagtca taaactgtct gattaaacac
    28861 caacaacttt ccgtttctgt cataaaccaa tcctcttgaa gggtagtcaa ttattctgcg
    28921 aagaacgttt tcatctgccg acaacttata tttatcttca attacctgta aatagaacaa
    28981 acgcccgata taggtaatag ctaccaatgc aatagtaatg agtaaaacga ggcgttgtgt
    29041 gctatgcgaa gttttcaaaa ttcagaattt tagcgttgca cttttgtttt atcgaacaat
    29101 aataaaatga taaaaataat tacaacagat gtaatggttg ataaacatac tctgaggaat
    29161 gttgctccga aatcgttaaa acggaacact tctgtataga acaaaaacag atgatgcaga
    29221 aaaatactga taaataaata atacaccatc cacaaaatgc ccatttgttt taatgagatt
    29281 acagcatctg agtcatagcc atcacgaggg gaaagaagct taatgatgta gggacgcata
    29341 aaaccaagaa acaccgatgc agctgcatgc atacccagtg tatcagaaaa aatatctaca
    29401 cttatacctg tgataaaagc aagaataagc aataatgttt ttggtattcg aacgggaagt
    29461 agcaacagaa ataatgggta aacaaaagga ttgatatacc ctgcaaatcg gattttgtta
    29521 agcaggataa cctgcagcaa tatcaacaga aaaaaacgta aaacagattt aattacctcg
    29581 ggtagcatct ttatgttttt cttctaattg tttacgttcg tttttcagca ggttgttcac
    29641 cacatacaca taagacacat cattaaaact tgtgctgagt ttaagtataa ttctataaga
    29701 gttgcttcct gcatcaatat caaaagattt taccgtaccc accataatac cctcaggata
    29761 gtagggcgaa aatgtggtag tcaccaatgt gtcgccaact ttaaaaggca catgtcgggg
    29821 aatgtcaatt acggttgctt ctgtgggaga attatcgcta tcccatacca ttgaaccatt
    29881 gtagttgttg tttttaaaac gcacactcac cttactttgt gtatgaagaa atgaagtaac
    29941 ggtgctgtaa tgtgccgata catttttgat gataccaata attccatgac ctgttataac
    30001 acccatttcg ggagcaatac ctgcatcgga acctttgtta agtgtcatat aattgttgcg
    30061 cctgtttgta ctgttgttaa tgattttagc ctcaatatag gaatattcaa gattgtgaat
    30121 ggtgtccact ttatgatttc ttacaacggt tgtatcgaaa tatgatgcag gaagcaaggt
    30181 gcgcaagtgt gcattttctt cggcaagcct tctgttgttt tcagccagat ctaaataatc
    30241 ggttacagaa cttatagtgg aataaacaga tgctacaact ttattggttg agtttagtgt
    30301 ggctgcattc tgataagagt tattctgtgc aatgaggaag aagcaaactg cctcaagcag
    30361 tataaacaat atgaaaatat aattccggct taaaaacagg aataaattac gcataaacta
    30421 taatttgcag aatgaaaccc aacctttaca aaggtgcaaa gttaattttc tgagcaatgt
    30481 tgaccattga aaagttttaa acgaggttat taatattttc tgacaaaaat caccacacat
    30541 taatccccca taatgtgctt gccgttgact attttcgcaa cattaatttt aatagtgcca
    30601 tattggaaaa aaagttaagc gagttaaagc cgggcgaggc aggggtcatt cagaatttca
    30661 gtgatgaaga gataaaactt aaactgatgg aaatgggttg tattccaggt gaaacggtgt
    30721 tgatggaacg agttgcacct ttgggcgacc caatggcaat taaagttgca ggctatcagc
    30781 ttagtttgag aaaaaatgag gcagaaacag tatgggtaag tacattataa atagaggggg
    30841 aatataagtg agtgaagttg ttctaaaagt tgcattggtt ggtaacccca atagtggtaa
    30901 aagcacgctg tttaatgtat tgacgggtct caatcaaaag gtggccaact ttcccggtgt
    30961 gacagttgag aaacatgtag gcttctttag tttacgcaat gcatttgaag gtgttgatta
    31021 caaagttgag ctaacagatt taccaggcat ttacagcttg taccctaaaa cacttgatga
    31081 gcaaattcca tacaaagtac tgaccgatcc gcaagaccca tctttcccca acaggataat
    31141 tgttattgct gatgcatcta atttaaaacg cagtttgttt ttatgctcac agataattga
    31201 tcttaaaatt cctgttgtgc tggtgttgaa catgatggac ttagctgttc ataaaggttt
    31261 gttgcccgat gtaaaaaaat tggaacaggc attaggcatc aaagttattc cggcaattgt
    31321 acgtcagaaa acaggtattg aagaaatcaa gaaggctgtt ctgcatacta ttcctttgcc
    31381 cgaaagtgat attatgcctg caacaaccct ggctcctgaa cttattcagg aaatcaaaca
    31441 aagttttccg gttaaaagtg attatgcagc ctacctgctt gcaggtaatt atgataaggt
    31501 ggacataagt tttgttggta atgaaggtaa acaaaaattg gacatgcttt tacagaagca
    31561 tcagttcagc caaaaaacta tgcaagctac tgaaacattg gagcgttata aaactatttc
    31621 tgttcttctt aaaagttgtg tcagcgagcc tgcatcagtg cgtaaatccc tgacccgtac
    31681 cgttgataat ctggttacac acagaatatg gggctatgga atttttctgg ctattctgtt
    31741 tgtaatcttt cagtttatat ttaatgtggc acagtttccg atggatttta ttgacggaac
    31801 ctttgctgat ttttctcaaa aagtaagcga attgcttcct aaaggtgtgt tgaatgattt
    31861 gattgtaaat ggtattcttg caggtcttgg tggcatcatc atatttgttc cgcagattgc
    31921 cttattgttt ttatttataa ccatccttga agacacaggc tatatggcac gtgttagctt
    31981 tattatggat aagttgctca ggccatttgg attaaacgga aggtctgtaa ttccattgat
    32041 gagtggtgtg gcatgtgccg tgcctgcaat ccttgcaaca cgaaccatac aaagttggaa
    32101 ggagcgtatt gtaacaatta tggtaacacc attgatgagt tgttcggcac gactacctgt
    32161 ttataccctg ctcatttcat tggttattcc acgatattat gtttttggat ttatcaattc
    32221 tcaggggttg gtgatgatgc tcatgtatct tatcggtttt gttgcagcta tattttctgc
    32281 atggttgttt aagatgtttg ttaaagccaa gcagcgtgat ttttttatca tggaattacc
    32341 ggtttaccgt ttgccacgat ggaagaatat cttctttgta attttcgata aagtaaaaat
    32401 atttttattc gatgcaggaa aaattatcat tgccatttct attgtacttt ggtttttatc
    32461 atctcgtgga ccgggaaaag attatgccga aacagaaaaa ctatatgaag caaaggcaga
    32521 acttcctgaa acaacacagg acgaactgaa ccaattacag gcacagaagc tggaacattc
    32581 ctatgcagga tggacaggaa aaattattga acctgcaatt aaacctttgg gcttcgactg
    32641 gaaaattgga attgccttgg tgacatcatt tgcagctcgc gaagtttttg ttggaaccat
    32701 gtcaaccatt tacagtgtag gtagtgaagg tgaaaattta ccaatcagag aaaagatgat
    32761 ggcagaaaaa cgacctgatg gaacacctct ttatacatta gctgttggca tttcactgat
    32821 gttgttttat gcatttgcca tgcaatgtat gagtactctt gctgttgtta aacgagaaac
    32881 cggcacatgg aaatggccaa tcattcagtt tgtgtatatg agtgctttag catgggtatc
    32941 aagttttgct gcttttcagc tactgagtta atttattatt tttcgaccga aattaattat
    33001 ggcaaaagtt actctcgagt ttatagaaga agcattaaag aaatatgttc cgcatcaatc
    33061 tatcagatat tgcatacaat ggattgaaga acatcagatc aatcttaaaa ttacaagaag
    33121 ccggaatagc aaatatggtg actatcgtcc gccacaaaga ggcttcggcc atcgtatttc
    33181 tatcaatcac gatcttaatc cttatgcttt tctcatcaca ttcattcatg aagtagcaca
    33241 tctaaatcag tggaaacgca agaaacaaat tattattcca cacggaaaag actggaaaga
    33301 agagtatcgt aaactgatga ttcccattct acgcgagaat attttcccgc ccgatgttgt
    33361 tgctgcactg aataattata tgacaaatcc atcagctaca agttgctcag accatgactt
    33421 gcttagggca ttgcgcaatt atgacagaga agaagataaa tggattacac tggaagaact
    33481 gaattttggt gtcaagttta aaataagaac aggccggact tttattaaga aagaaaaact
    33541 acgtaaaaat tatagttgta ttgattgtcg ttctaaaagt atttacttca tcaatcccat
    33601 aacggaagtg atgcctgtag atcattgaac aaagaaacga tcaggaacat taataagata
    33661 aacttttttg cgtgcacgtg ttattgctgt atatagccat cgcatataat caacaccata
    33721 catttcttct gtcataaagc ccggatcaat aaacactgtt tcccattgtc cgccttgtgc
    33781 tttgtgacct gtaatagcat aggcatattt tacctgcaat gcattgagat aaggatcttc
    33841 tcgtagttta cgcatgttta ctttgccatc tgttttgcga taatgatttc tcacttcatc
    33901 aaacaatttt ttatactcat cataagttaa tgcaggcgac tcgctgttta atgtatcaag
    33961 caaaagtttt acttcaagcc ttgttgattt ttcatcagat aattctattg tagcattggc
    34021 aaaacgaaac ccaaaatact cttcctgctt ttttaaacga atcagtttaa tttcatcgcc
    34081 attggcaata aatgcattgt cattgtctga ttcatgccag tagtaattat tttttacaac
    34141 catcagcctt tcgtttttct ctactacctc ttcaaagcca taaaaagttt ttctgataaa
    34201 ttcattgtat cggtttgcct gcttattact tctgcacaac acagttactg tgttatcatc
    34261 accggaaggg aaatatcccg aaagtgcttc agagagctct gctgaagtta cttggtttac
    34321 atcatcaaat cctgatgttt ttaggcgagg aaaatgctct tctctttcaa gcaataatcg
    34381 tgtgcaatgt gcattagcta atattccgga gtgaagttgt tgccgcacca cttctttcaa
    34441 ttcatattgc cttacctgaa gctgacagtt gttttgaaga aaattctgat ccaatgcagg
    34501 actgcattgc aaaccaacgg ggggcaattg tgcagtgtcg ccaataaata ctatagcatt
    34561 gtctgcctga ctgtaaacat gatctactaa gtcagaaagt aaacttgctc ctttaaacaa
    34621 tgtcttatca tcttcattcg aaatcattga agactcatca ataataaaaa tctgcttttt
    34681 aaatttgttt ggctttacct ttgttttgat gtcgccattt ttatcacttt ttatttcata
    34741 caagtaccga tgaattgtag aagcatcatc accggtataa tggttaatca ctttagctgc
    34801 tctgcctgtc ggggcaagca ttatcactct gaaatcgtaa tgataaagtg tttttatcat
    34861 tgcattaatt accgttgttt ttcctgttcc ggcatagcct tttaaaagaa agcacttata
    34921 tgcagtattt tctgtaataa actccgatag tcctgcaaaa gcatgcttct gtgcattggt
    34981 aggttcaaat ggaaaattct tcagtaagag attaaaaact gcagcaggct tcaatgttta
    35041 tcttgcatta ttatcggata aaaatacaaa aggggaagtt aacatcataa gatagataac
    35101 caatgataat aacaatgatg taaagagttg ttattactaa acaaacattt aattagtaga
    35161 ataatatttt gaatcccaca ctctgaattt ttaattatca cctcattaat ctttgtactt
    35221 tcgtgcattc ttaaaattgt aatacatgct cagcatagac attcacacac atatactacc
    35281 ggagaaatta ccggattgga agtcgaaatt tggctatggt ggttttatca cattagatca
    35341 tcacaaaaat tgttgtgcca ggatgttgcg tgatgacggc actttttttc gtgagattga
    35401 aagtaattgc tgggatgcaa agacaagaat gaatgactgc gatgtacatg gtgttcatgt
    35461 tcaggcattg agtactgtac ctgtcatgtt ttcatattgg acaaaacctg cagatggact
    35521 tgctgtatca gaatttttaa atgatcatat tgcaacaatt gttcaacaat acccaaaacg
    35581 ttttgtaggc ttaggtactg ttcctctgca agcaccggat ttagctataa aggagatgga
    35641 aaggtgtaag aaaattggac tgcaaggctt acaaataggc tcgcatgtaa acgactggaa
    35701 tcttaatgat gcaaacctgt ttccgttttt tcaggcatgt gcagaaaaca acatgccgct
    35761 ttttgttcac ccatgggaaa tgatgggtca aaaagatatg caacgttact ggctgccttg
    35821 gttggtggga atgccggcag aaacttctcg tgctatttgt tcaatgattt ttggaggtgt
    35881 gtttgaaaga ttgccaaact tgcgtgttgc ttttgctcat ggcggaggaa gttttccgtc
    35941 aacattcgga cgcattaaac atggttttga ttgccgcccc gatttggttg caatagataa
    36001 tgcatcttca ccggagagat atatcggtaa gttttatttt gactcattgg tacatgatga
    36061 aaacatgttg cattacctga ttaatttcac aggagccaat cgtattgcat taggaagcga
    36121 ctatcctttt cctttaggtg aaaatattcc cggaaccctg attaaaaatg caaaactcga
    36181 tttagaaact aaagaacgat tgcttggtgg cacagcatta gagtggttgg gtttatcaaa
    36241 agaaatgttt ctgagataga ttttctactt tcattcaatc tcccaaaaca atgttaaaat
    36301 tgttaaagac ttcttaaatt ttttctcgga aacttaaaaa actaaaatag tttccatagt
    36361 tttgcccagt ttttaacaat tatcatggat ataagagaaa gaattttaga gtcggggttg
    36421 gagttgtttc tggcacatgg tgtcaggcgt ataaccatgg acgacatagc aagacatttg
    36481 tccgtttcta aaaaaacaat ttatgcctgc tttgatgata aggatgaaat tgtatctgaa
    36541 tgttgtcagg gatatttaaa aaacagccgt tgcgagtttg aacatgttga aacagaaagc
    36601 aaggatgcca ttcacgaaat atttctgaca atggaaaaat tgcaagcgat gttcaccacc
    36661 atcaacccag cattgtttga agacttacaa aaattttatc cgcgagtgtg gagtatgtat
    36721 gttcagttta aagataaaca tgttgcagaa gtcattgaaa gaaatctttt aaaaggcatt
    36781 gagcaaaaac tttaccgtcc ggatatcaat attaaaatat tggtaaaact gagaatagaa
    36841 gaaattactc tgggaatgaa tactacagtg tttcctttaa agaactttaa aacgcaggat
    36901 gttcaggttg cattgctcga tcattatctg catggcattg taaccgttaa aggacataaa
    36961 ctcatcaata aatataaaaa cataacagac gaagaataaa taacaaccgt atgctgaatc
    37021 aaattaaatt tcttacaggt ttactgttgc tgatatcaat caatatacag gcgcagcaat
    37081 ccgataaaaa gtcttattcc ttttcattga aacaggcaat tgattttgct tttcaaaatc
    37141 agaccgatat tcaaaatgca gttctcgatc agaagtcagc acaaagcaga gctaatgaat
    37201 tattgggaat tggtttaccg caaatcagtg ccagtgccga catcacacat tttattgaca
    37261 taccaacaac ctttgtgcct gcagaatttt ttggtggcga gaaaggtagc tatgcccccg
    37321 tacaatttgg ccagccatat agctctactg ccggtttatc tgcatcacaa ctattgtttg
    37381 acggatctta tttagttggc gtaaaagcat cacgcatgta tgccgatttg atgcgtaagc
    37441 aagttattca gactaaaact gaaaaagctg cactcatcag caaggcatat tataacgtgc
    37501 aagttaacag tgagcgtttg cagatgctcg atgcaaatgt taagcgatta aaaaagcagc
    37561 ttgatgaaat gcaggtaatg tacaataatg gttttatcga aaagattgat ttagaccgaa
    37621 tcagtcttgc ctataacaat attgaaaccg aaaaatcaaa tgcggacaga ctgattgtgc
    37681 ttgcacaaaa cctgttgaaa tttcagatgg gattgaatgt taatgatgag ttgacactca
    37741 cagataaatt ggaagacata aaagttgttg ccgacaataa cctgaatcag aacattgata
    37801 taagcaacag aatagaaatg gatgttttga atacaaccaa agaattactc agagctgatt
    37861 taagattaaa aaaatcttct taccttcctt cacttgcagc ctttggctcg tatagtgtaa
    37921 gtgcatcgcg caatgaattt gatatttttg aaaacaaacc ctggtttcgt acttcaataa
    37981 ttggcttgaa acttaacgta cccatttttg atggctttca gaaggcagca cgagttaatc
    38041 aggcaagatt tgcgcttaat aaaatggaga atcaaattca gactgcaaca caaggctttc
    38101 aattagaagc gcagactgca aagacaactc taaccaatgc attaggctct cttgccaatc
    38161 aggaaaaaaa cagaacatta gctgctgatg tagcaagaat tacaaaaata aaatatcagc
    38221 aaggtgttgg cagtagcctt gaagtaatgg atgcggaaac agctttgaaa gtagcagaaa
    38281 caaattatta tgctgcaatg gtagatgcat ttattgccaa agtagatttt gagaaagcaa
    38341 caggaacatt aataaaataa aatataaact ctgacataaa taaatatatg aaaaaggtaa
    38401 ttatagcaat aacagcaatt ggatttctta ctgcctgtgg tggaggaaac agcagtaaaa
    38461 aggttgagga acttgaaaaa ctcagaaagc aacagtctga gttaaatgaa aagataaaat
    38521 ctttagaagc agaaattgca ctcacagata cttccagaaa agacaaaata aaatttgttg
    38581 cagccacaga tatgagtttg aaaccattta ctcattatat tgaagtgcag gcaaatgttg
    38641 aaggtgatga agatgttgta ttgagtgcaa aaactcccgg aactgttacc tctgtaaatg
    38701 taaaagctgg cgaccatgta accaaaggtc aggtgttggc aacgctcgat gatcagatta
    38761 tgcagaaggc aattgcagaa gtacagaatg aacttaatct ggtgacaaca ctttataacc
    38821 gtcagaaaaa tctgtgggac caacagatag gtagtgaagt acaatatctg gaagcaaaaa
    38881 caagaaaaga aggtgcagag aaaaaacttg catctattca ggagcaatgg gatatgactc
    38941 gtattaaagc accattcagc ggaacagtag ataatgtgat gataaagaca ggacagttgg
    39001 tttcaccaca ggttcctgca ataagagttg ttaatctcga aatgcttaaa gtaaaaggcg
    39061 agttacctga aacttatatc ggaagagtaa ataaaaatga taatgcattg gtttattttc
    39121 ctgacttagg aaaagaaatt acaagtaagg tttattattc cggaaatgcc atcagcaacc
    39181 tgaaccgtac atttaatgtt gaggttcgtt ttggcagtaa agaaaatttc ctgcgaccta
    39241 atatgcttgc cgttttaaaa atagctgatt acaataaaga gaaagcaatg tcacttcctg
    39301 ttagtgttgt acaaaaagga cttgatggtg agtatgtttt tgttgctgaa aattcaggag
    39361 gcaaaactgt tgcaaagcgt aaaacagtaa aaagcggaat gacatataat ggcaatgttg
    39421 aaattgttga aggattgaat gaaggtgata aagtgattac aacaggttat cacgatttgg
    39481 ttgaaggcga tatcattaaa ctataaaccg caagcatttt atgaaagata tatttaaaga
    39541 attcaagccc tcatcatggg caatagataa ccgtacggct atatacatta ttaccgtcat
    39601 cattacattg acgggtataa tgtcatacat caatattccg aaggaaagtt ttccagacat
    39661 ttctttacca aatatttata tcagtgttgt ttatccgggc acttcaccaa aggatatgga
    39721 gaacttagtg gtgcgccaca ttgaaaagga gtgtaaagga atttcaggtg taaaaaaaat
    39781 caagagtaat gcactgcagg attattgtag tatagtggta gaatttaaca gcgatgtaaa
    39841 gattgaagat gcaaagcgaa aagttaaaga tgctgttgac cgtgcacgta atgatctccc
    39901 gcaggattta cccaacgagc ctgatattat agaagtcaac ttttctgatt tgcctattat
    39961 ggctgtaaac ctttccggtg attatgatct tgcaaaatta aagcgctatg ccgatgaagc
    40021 taaggataaa atagaaagcc ttaaagaagt taaagaagtt aaactgattg gagagttaga
    40081 gcgtgagatt cagataaatg ttgacatgct taagctgcaa tcatcaggat taacgatgag
    40141 tgatattgaa cgtgcagtta agtacgaaaa tatgactgtg cccggtggcg gtgtgcgcat
    40201 ggccgatggt acacgcaggt cgttgagtgt ttcgggtgag tttaccaatg ttgatcagat
    40261 aaaaaactta gttgtaaatt ctattaatgg tgcacctatt tatttaaaag atattgcaga
    40321 agtagttgac tcctacaaag agcaggaaag ctatgcacgt ctaaatcata aaaatgtaat
    40381 tacactaagc attatcaaac gctccggtga aaacctgatt gatgcatcag ataaaattgt
    40441 ttctgttgtt aatgaactta aagcagaagc ctggccaaaa gatttgaatg ttgtacttac
    40501 aggtgatcag agtgaacaaa cacgtacgca gttgcacgac ctgattaata ccatcatcat
    40561 cggttttgta ttggtattaa ttgtacttat gttttttatg ggaacaagca atgcattctt
    40621 tgttgccatg tctgtgccac tatctatgtg tgtggcattt ttggttttac ctgttatagg
    40681 atttacaatt aacttcattg tattattctc ttttctgctt gcattaggaa ttgttgttga
    40741 cgatgcaatt gttgttattg aaaacacgca tcgtattttc gataatggga aaatggatat
    40801 tgttagagct gcaaaaatgg caacaggcga agttttctta cctgtgcttt ccggaacact
    40861 caccacactt gcaccttttg ttccattagc attctggaaa ggcatcattg gtaagtttat
    40921 gttctttctt cccattaccc ttatcataac attactggca tcattggtgg ttgcatacat
    40981 catcaaccca gtgtttgctg ttagttttat gaaaaagcac gaagaaaagc atgacccata
    41041 caaaggttta aaaattactt caataatttt tattgcaatt gcactgttgt tttatgtgac
    41101 aggtgcattt ggcatgggta actttacaat cttcattctg ttgttaaatt tacttcaccg
    41161 tttttggttg cataaagtta ttgtacgttt tcagacaaaa acatggcctg cagtacagga
    41221 gtcatataaa agaatgttga ttaaagttct tcaccgccca cgcactatga tggcaggaac
    41281 agtaggattg ttgtttttat cattcattgc aattggtctt aggaatgggg gagtaaattt
    41341 tttcccaatt gcagatccca actttgttta tgtgttcaca acattgcctg ttggaaccga
    41401 tcaggcctat acagactctg taacaaaaat tcttgaaggt aaagtttata atgtgttggg
    41461 cgaaaagaat gatgttgtaa aatctgttat tgccagcgtt ggtgtaaacg tttctgaccc
    41521 atcagacgaa gatcagggat actatgccaa caagagtaaa atagaagtag catttgttga
    41581 gtacagtaaa agacatggtg tttcaactag taattattta gagaaaatca gagaagcagt
    41641 aaaaaatact gttcccggtg ctgaagttac tgttgcacaa gagcaaggcg gaccacctgt
    41701 aggcaaacct atttctattg aagtgagtgg tgatgatctt caggcaataa ctacaacttc
    41761 taaaagcttg aaacgatatc ttgattcctt gcagattgaa ggtgtagaag aattaaaatc
    41821 agacctgcag gatagcaaac cacaaatagt gtttgacatc aatcgcgaac acatgaaccg
    41881 tcttggaatt atgacaggta ccgtaggaaa tgaaatcaat atggctgtac ttggtcgtga
    41941 ggcttcaaag taccgcgatt taaatgatga gtataaaatt atgttgcgtt ataaactgga
    42001 tcagcgatat gatgttagtc tgctcaacaa tttaaaaata ttgtatcgtg atatgtcaat
    42061 gggaggactt gtgcgcaatg taccgttatc atcttttgca acggtgaaat atgataacac
    42121 ctatggtgtc ataaaaagaa aaaatcagaa gcgtgtagta acagtttcat caaacctgct
    42181 taccgattat caaagtcgcg aagcagaagt ggtgagtaat gtaatggctg ccgtaaaaca
    42241 atttccaaaa acagaaggag tagaaataaa atttgcaggc tcgcaggaag aacaggcaga
    42301 aaccatgtca tttctcggaa cggcattaat gatttcttta ggattgatat ttatgatatt
    42361 ggtaattcag tttaactcta ttggcaagcc ggtaattatt ttgtcagaaa ttgtatttag
    42421 tatcataggt gtattacttg gatttgcaat ttttaaaatg ggattctcga tagtgatgac
    42481 tggtataggt gttgtagcac ttgcaggtat tgttgttaga aatggaattc tgttggtaga
    42541 gtttacagag ttactgcgcg aacaaggtgt tccaacattt gaagccattg tagaagccgg
    42601 tcgcacaagg atgactcctg ttatacttac agccactgca acatcattag gactaattcc
    42661 gcttgctgta ggtttgaata ttgacttcgt aaaattgttt acggagttta atcctcacat
    42721 tttctttggt ggcgacagca cagccttctg ggggccacta tcatggacaa tgatattcgg
    42781 attgatgttt gcaacattcc ttacactgtt tttagttccg gtaatgtatc tgatgtcaga
    42841 aaatctgaag gcaaaaattt acaaaagacc aaagccgtta atgaaaaaac cggatgatga
    42901 attgattgcc tgatagaatt ttttaaagtt tataattgaa gaaggccgtc tcagaacaat
    42961 ttttgagatg gtcttttgat ttatggtata tttactttgg agttcccccc aaaaaaaagg
    43021 tactatttgc aaattaccat aagcattact ttgttgtcga attttaattc acattttaaa
    43081 atagtctctc attacgtcat agttgatttt tccaaaataa aaaaggctgc tctaaatcag
    43141 agcagccttt ttttaatcac tattttttat ctcgaatact gagcttctaa ctgatcaaac
    43201 cttttctgca agtcgtcagt aatagctttc tggtctttga gtttcgacag cctgattaac
    43261 tcctgaaaaa ctgccagcgc ctgattggtt tcgcgctcta cacctttggc ataaggtgtt
    43321 cctttaagcg ataaataata ttgcaaatcg ttctgataaa tatctgccag tcgttttaca
    43381 attgaattac ctttttcaat actctctttt gaatctgttt tcttcagttc aacatcattg
    43441 ctaagcatct gcaaactgtc aggcaatgca ttactcattg tactgcgata atacaattct
    43501 gcaattcgca acatcataat gttatatgga actgttttat caggcatttc taccagacat
    43561 ttgtcaagaa ctttcactgc agaatcaggc tcgttttctt gaagtaatgt ttcgctcaga
    43621 cgtgcaaaat tattacggaa gttcatcgtc atgttcaggt tgttctgatc gagatatact
    43681 ctttcatctt tcatattacc ccacataaac ttattcatca tattgtcgta catgcgttct
    43741 gcggcaacac ttcctgtctg tccggatggg tcggcagcag ctttaatcgg aacaaaacga
    43801 taagccaatc cttctgtctg aaaataattc tcaagattaa gataagaatc attgccaacg
    43861 gtaactgcaa aataaatagg acgtgtccag tcattgtgtg caataagatc caatatcatc
    43921 aggtcagctt tcatgatata gtttttgctg atttcaaatt caatataatc aacaatcttg
    43981 cttgtatctt ttgcctccac cacattgttc ttaatgacag cttgtttgtc aacttttatt
    44041 ctgaactttt ttgtaggcag ataattaaat tcatcaccac cttcgctgcg cacttttgct
    44101 tcggggttat cgctggtaat aaattcaata atatctttca ggtcgctgta tccctgcaca
    44161 cttctgtcgt agaatggaat ataatcacgt gttccctgaa catactgttt tgtatttaat
    44221 gtaaaaggaa cagggtctga ttcataagcc ttacgtttta tctgatcaat ataccagtct
    44281 gtgttgagga gactcaggtt tataacacgc acatcggtac gcacaccttc aacatcctgt
    44341 atgtaccaca acgggaaggt atcgttatcg ccattggtaa aaattactgc atttggagca
    44401 caggagttca gatagttgta tgcaaaatcg cgtgaggtgt aacggtaaga gcggttgtga
    44461 tcatcccatc cttctttagc cataactgtt ggcacaacaa ggcagagcaa cgtagccagc
    44521 attgccgcag ttgtttcagg cattcgcttt tttaaagcat cggcaattgc tgccactccc
    44581 aaacctatcc aaatacaaaa tgcataaaag gctcctgcat aagcataatc acgttcgcgg
    44641 ggctgataag gtgtctgatt caggtaaatt acaattgcaa ggcctgtcat gatgaaaagc
    44701 aacatcacaa cattggcatt gttcatgtct tttttatagt gataaaccaa tcctgcaaga
    44761 cctaacaaca gtggcaagaa gtaaaatgta tttcttgcct tattattttt catactgtcg
    44821 ggtagtttgt cctgcggacc taaaaacata ctgtcaataa atggtatgcc gcttatccag
    44881 tttcctttta atatgctgcc atgtccctgt atatcgttct gacggcctac aaaattccac
    44941 ataaaataac gccagaacat gtggttcatc tgatagctga aaaaatattt gaggttgttg
    45001 ctgaactcag gcttttgttc accttttatt cctgaccatt ctttgtatgc cgaaacatgg
    45061 ttaggctctc ggctgtacat tcttggaaaa acggtacaac gtgcaggatc gtattcggga
    45121 ataaccttgg ctcctgtcgg aatatatttt ccgtcaatag cggtgtagct cattgcgcct
    45181 tctgtctgac ctacaacttt ggcatcgtaa tattgtccat agcctaaagg tctgtcgcca
    45241 tactgctcgc ggttgaggta gcctaatagt gcaaatgcat tctccggatt gttttcgtcc
    45301 attggcgggt tggctaagga gcgcactaca atctgtgcat aggaagtgta tcccaacaac
    45361 agaaatgcaa agctcagcaa aatagtgttg agcagtgctt tttgtttttt ctgagaatag
    45421 taaataccat aagtcagtgc agctaaaatg atgagagagt aaacaacaaa tcctgtccat
    45481 aaagatgagc ctgactgatt gaaccataaa tcaatttttg cgcctatgtt tacaaaccct
    45541 gcaataattc catattgtac aaccattaaa attaccaatg ctatacccat agtttttgca
    45601 acacccatcc acgaaaacgg atatttgcgg aagtaatata ccaatgccaa agcaggaata
    45661 caaagtaagt tcagcaagtg aacaccaata gacaagccca tgaggtaggc aatgaggatt
    45721 atccaacgga tgctgtgctt ttcgtctgca acattttccc atttaaatac agcccaaaca
    45781 acaatacagg taaagaactg tgatgttgca taaacctcgc cttcaacggc agaaaaccag
    45841 aatgaatctg tgaaagtaaa tgccagagca cctacaacgc cactggcaac tacggttaaa
    45901 atctgtgcta atgtaggctc ctgatttttt acgaaaacaa tttttcttgc aatggcagta
    45961 attgtccaga aggtaaacaa tacggcaaat gcactcataa tagcactgaa tgcgttaacc
    46021 attagcggca catcttttac atcggcacct gcaaaaagag tcataatgcg tgagagcatc
    46081 agaaataatg gcgctccggg aggatggcct atctgtagtt tgtaggccgc agcaataaac
    46141 tcgccacagt cccaaaaact acctgtcatt tcaatggttg acagaaatac ccatgctgca
    46201 atggcaaaaa ctgcccagcc ggaaatgttg ttgaattttg tatattgttt catgtaaaac
    46261 aaagaaggtt acaagcgtgc gaaaatagca aattatgctt ctgaatggct tttaatttta
    46321 aataagcttt aggtaccact atgaatcttt aagtggtagc aagaaaaaca tgtcattgct
    46381 tcaccacctt aacccgtgct gcgttatacc cgtctaacac caacacagca aggtatgtgc
    46441 ccgaagtata gtttgttaaa ttaagctgca ctgtgttact gcctgtttgc aagggttgct
    46501 ctaaaagttg tttgccacgc atgtcaaaca aaataatgct tgcgcctttt ttgccaatgc
    46561 ccgtgctgtt gctttgaggc actatggctg taattgtaca ataggttgtt gcaggattag
    46621 ggtaaattgt aagcgttgct gttttaaaaa tttctttttc ttttatgcct gtaaccaaca
    46681 aacctaagct atctgttttt acaagccaaa aacatgtgcc gccattgttg ttgcagccac
    46741 ctgtttgccc gcacataata taaccgccat cggcagtggt ttttatgtcc caaaaatcac
    46801 tgtagctgct tgagccaaat tctctgtacc ataagctgtc tccgttttca tcaaggcaat
    46861 gtatgtatgc ttttgctaaa ttactcatgt tgtcagatag gccggcataa atataaccgc
    46921 cattaaatgc atttgctaaa cctaatgagc tgcctatact gttaaatggt aaataatttt
    46981 ttttccaaat cattgcaccc gcaagatcaa tttttgcaat tgagttaaaa tatggttgaa
    47041 ctgagccaga actgaaattt tcagagccaa taattattat gttggtatca tttgaataaa
    47101 tcatttcggt aaaagctgaa ttaccaatag taggtattgt gaaaaaagac aattgatttc
    47161 caaatgaatc aacataaaaa taaaaagcat ctaaatctcc attaattgtg ttaatgttat
    47221 tacccgtaca cataaaacta tgatcaggca attctatcac tccccatatt gcctgataag
    47281 g
//
LOCUS       DAOOOI010000010        46765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_121_length_46765_cov_57.458724, whole genome shotgun sequence.
ACCESSION   DAOOOI010000010 DAOOOI010000000
VERSION     DAOOOI010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 46765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46765
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121_length_46765_cov_57.458724"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..682
                     /locus_tag="PKX59_03050"
     CDS             134..682
                     /locus_tag="PKX59_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOZ89875.1"
                     /translation="MTTDVTNIWTNFNKELKGFIYNKTRNSADTDDILQEVFVKIIRN
                     IEKVNQSENLRQYLYGIVRNTLNDYFKNQKTFSSDTEIQEQFSETETNSLNETIAECC
                     IKPFINKLPDNYKQALLITEFQDISQKELAKKLNISYSGAKSRVQRGKDKLKELILNC
                     CAYQNDKYGNLIDTEDKSCGCS"
     gene            756..1688
                     /gene="arsM"
                     /locus_tag="PKX59_03055"
     CDS             756..1688
                     /gene="arsM"
                     /locus_tag="PKX59_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOZ89876.1"
                     /translation="MNTEVIKEEIKKSYGDVAKGNIQVGCCSTETGCCTTSEFSNSMS
                     EDYSNVEGYVKSADLALGCGLPTEIANIHEGDTVIDLGSGAGNDAFIARRIVGETGKV
                     IGIDMTPEMVIRALQNTQKLGYKNVEFVLGEIENMKEISNNVADVVISNCVMNLVPNK
                     EKAFNEVNRVLKVGGHFSISDIVFQGAIPQGVLEASEMYAGCVAGASEKGQYLGIIKK
                     AGFKNISIKKERLLELPDELLLKYLNQQELDDYKKSGSGIYSITVYADKLEEGTCCDT
                     TENGCCGSETKTEKTTEKTACCGTTSNGNSSCCG"
     gene            complement(2052..2564)
                     /locus_tag="PKX59_03060"
     CDS             complement(2052..2564)
                     /locus_tag="PKX59_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOZ89877.1"
                     /translation="MSLDSKLRSLAFHHQEYLNIGTTLRAYCKKHYTRDYNLLRSIPG
                     IGGFLASAVIAECGDLRRFANEKQFSSYIGVVPGIYSSGGNETMLGVTPRSKSLLRTY
                     IIESAWVALRYDQEIQAYYRKHASKNPKSVIVKIAHKMVKRMLSVIKNGQPYRVNNTI
                     NKLKEEKEKQ"
     gene            complement(2580..3140)
                     /locus_tag="PKX59_03065"
     CDS             complement(2580..3140)
                     /locus_tag="PKX59_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOZ89878.1"
                     /translation="MQTQQTSSTPKVIIGMDIHKKSWSVSIRTDICDHRTLNMPADAD
                     GLYDYISSNFPGHEVSIAYEAGCCGFSAARHFIGMGWKVLVVNPADVPRMDKQNYQKT
                     DKIDSRNLCKQLYNNNLRGIYIPTSEEEELRSLVRHRNQIVKQLRKVKTHIKSMLLFH
                     GITIPIDFDNNNWSKAFIKWLKNLNW"
     gene            4078..5385
                     /locus_tag="PKX59_03070"
     CDS             4078..5385
                     /locus_tag="PKX59_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89879.1"
                     /translation="MPKGTRKTKFVKHTLLTFIYFALTSNSFGCSGYKITLGNKTILG
                     SNEDAWRLTPQIWFEKGKGNSQYGAAFTGSRYDGENGYAPQSGMNEMGLSFERLASYH
                     PIQEKFTNRKAITNPTKYLKDILHTCKSVEEVKEFINKYDYSYFIEDVFFYVDKSGKY
                     LIVEPYTLTIGNEPTFVISNFCPSITSEQTANKLTRYRNGVAFLKNGFDTTIAFCTAL
                     SDTMHVCRKKIGDGTLLSSIWDLNSGTVNLYFYHNYNATVQFKLSDELRKGDHIIAIE
                     TLFPHSAEFEKLRNFKTPKDSNFMGLFIVASAGFFLLTSIFFLTQFFRRKESNKYTYI
                     QLLMFPIGLIMFYYMCVLCGSVNVFYFPAPYKDPANIFVSMTSYIPFLILLLILPFSV
                     VNLRILKDNSWSFVAKGLFTFNNIIYFVLIGLFTYWRFYSIFN"
     gene            complement(5551..7659)
                     /locus_tag="PKX59_03075"
     CDS             complement(5551..7659)
                     /locus_tag="PKX59_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ89880.1"
                     /translation="MKKKATVTKQESQAKVQSGINLTQLKKYLGLLLAAIAFLLYANT
                     LNHDYTVDDGTVMQNNKIVKKGVSAIPEIFTTAYRKGFWDRNESLYRPLSVAMFAVEW
                     QIAPENPSIGHWVNVLLYVLTAWVLFRFLVNLFKDDWLFAFIATLIYVIHPIHTEVVA
                     NIKSRDEILCFLFLILSFNSFLKFLDRAKPFYALGVLFYFILSLLSKESSVTAIAIFP
                     LIAYFFRQKTIGNSIKLSWSPAIAIVAYLSLRFVVLKGISNFNEILPINNSIVNAQNY
                     IQQFATAVMIAGKYLYLLIIPYPLSFDYSYNTFPVVNFSEIKALLSLTVYVFLLIMAI
                     KGFKSRSRFSFGIFYFLLTMSIVSNVFFLIESVMAERFTYLPSLGICIALTSVLFTLF
                     KVSNVVREKIDINKIIATNRIFFGLILIVFVVYSGMTVARNAKWKNNLTLLSTDVKTC
                     PESARIRYAYGSAILIEQALKEKDYSKKMNLLDKSIEQLEKGVAILPTYSEAYYHLGL
                     AYKERENYAKAIESFEYAAKSKTFTDPQFFISWGVACGKAKRYQESIKALNHAIDLDP
                     AAKEAYLNLGVFYDEMQESDKAETSLMKAIELDSLSDGAYYNLGNVFAHRNNFVKAIQ
                     NYKKALEINPTNEDAVNNTGNSYAAMQDYENAIIYFREAIELNPSNARALNNLGVTLV
                     LTGKKDEGEQYIAKAKAIGG"
     gene            complement(7731..9080)
                     /locus_tag="PKX59_03080"
     CDS             complement(7731..9080)
                     /locus_tag="PKX59_03080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOZ89881.1"
                     /translation="MSLPKEFLESLPLSESDKELFVQSLQKPVPVSVRINPQKHTTHF
                     SGSSVPWCRQGYFLEQRPVFTLDPLFHAGAYYVQEASSMFLEQVIFQLSLNEKPINVL
                     DACAAPGGKTTHLLSLLHQESLVVANEVIASRTPVLVENAIRWGYSNLIITRGDAVLF
                     KRLENIFDVIVCDAPCSGEGLFRKDEAAISEWSLQNVEHCALRQQRIVNDLWDALKPG
                     GYFIYSTCTSNLQENETNVQQFINKHNAECVKLKIKDFPGVEEFQRQDAVMYRFFPHK
                     TGTEIFSLAVLRKPDLNDLNSERVVAKNLKLADIVQADFAKKHIDKGEEMFYFIHDNN
                     IRFMPICLKNALGQLLNMPVIHAGTAAFEVKGKNLLPSFELALSTALNQENFNKAEVD
                     KSTMLKLLKGETRLMDSLPIGQHLITYQNIPLMFLKKIAGRVNVNYPRSWFIRMNIE"
     gene            9222..11075
                     /gene="yidC"
                     /locus_tag="PKX59_03085"
     CDS             9222..11075
                     /gene="yidC"
                     /locus_tag="PKX59_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOZ89882.1"
                     /translation="MDRNSILGVVLIMAVLIGFSIFNQPSKEEQEAAKRKQDSIALVE
                     KKHQDSLKLITKPVAALKADTVAVADTLNNDSLSQTKLTDNFGAFALAAQGESKSITL
                     ESDLLKLKLNSKGGKIDYVELKKYKTGEGKPLVLNNGDSSQFSLSFFSENKMISTGDL
                     YFDLPAKGITVTGKDSQSVSIKLNAGEGRYIEYVYSLKGDDYRVGFKINFVNMGSLVA
                     SNAGYMELNWNQKLLQQEKSHKNESAVSTIYYRYTDEEVDHLSETKDDGTELKTKVQW
                     VGFKQQYFSSVLIADQAFESPTRINSTVINDEGAVRMMHANFTIPFSHQNNESTGMSF
                     YYGPNHYQTLNKYHIGMEKLVPLGWGIFGWVNRFAVIPVFNFLNSFNINYGIIILLLT
                     ILIKVVLLPLTYKSYISTAKMKVLQPEMSELQAKFKADPMKLQQETMALYRKAGVSPL
                     GGCLPMVLQMPILIAMFRFFPASIELRQEGFLWAKDLSTYDSILDLPFNIPFYGDHVS
                     LFTILMTVSTLIYTRMNMQMTAATNPSMKYVMYLMPIMFLGIFNNYSSGLSYYYFLAN
                     MITFGQQYLFKSFVDEKAIHAKIQENKKKPVKKSKFQERLEKMAKERGVRK"
     gene            11302..12009
                     /locus_tag="PKX59_03090"
     CDS             11302..12009
                     /locus_tag="PKX59_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOZ89883.1"
                     /translation="MNKVVKNVDEALKDFRDGMTLMVGGFGLCGIPENCIAALVRKGI
                     KGLTCISNNAGVDDFGLGLLLQQKQIKKMIASYVGENAEFERLMLTGEMEVELIPQGT
                     LATRCMAAGYGMPAIFTPAGVGTEVAQGKEVRRFSYNGVEKDYLMEYAFDSDAALVKA
                     WRGDTEGNLVYRSTARNFNPMMAMAGKITIAEVEELVPAGTLDPDQIHTPGIYVHRIF
                     QGEKYEKRIEQRTVRKK"
     gene            12051..12419
                     /locus_tag="PKX59_03095"
     CDS             12051..12419
                     /locus_tag="PKX59_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOZ89884.1"
                     /translation="MSRKFDSPFEDNKILKLTFEFSLKIIHLSEILNSNHQYIIANQI
                     LKSATSIGANAYEAQNAESEPDFIHKFKIAAKETEETYYWLLLCKHAECYPDTTDLLI
                     ELEVIKKIINKIISTSKNKL"
     gene            12477..13133
                     /locus_tag="PKX59_03100"
     CDS             12477..13133
                     /locus_tag="PKX59_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HOZ89885.1"
                     /translation="MLDKNAIAKRIAREVKDGMYVNLGIGIPTLVANYIPKGINVTLQ
                     SENGILGMGPFPFEGEEDADLINAGKQTVTLLPGSVIFDSAMSFGMIRAQKVDLTILG
                     AMEVSEEGDIANWKIPGKMVKGMGGAMDLVASAKNIIVAMQHVNKAGESKLLPKCTLP
                     LTGIKCIKRVVTELAVLDVTPQGFKLLERAPGVSVEDIKKATAGKLIVEGDIPEMVID
                     "
     gene            13146..14555
                     /locus_tag="PKX59_03105"
     CDS             13146..14555
                     /locus_tag="PKX59_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional class I SAM-dependent
                     methyltransferase/glycosyltransferase family 2 protein"
                     /protein_id="HOZ89886.1"
                     /translation="MRYLDHFEKIAEKRIRDRHKHRYYWNDITFYCNYFSHETFSVLE
                     VGCGTGELLNNIRAAQKTGIDYSPTMIAEAKKQFPQIDFQVMAAENISLEEKYDLIIL
                     SNLTGYLEDVQAVLEQLKKVCHERTKIIVSYHNHLWEPFLRLAENLGLKTKTPQLNWL
                     TSMDVRNLLFLAGFDCYRESRRMLLPINIPLLSPLINKFIAKLPLFRLMCINTYSVAK
                     PLANATEDVNEKYSVSIVIPARNESGNIENGLLRIPKFGKWQEVIFIEGNSTDDTWDS
                     INEVSSKYAASHKIKVGKQEGKGKNDAVRKGFSMAEGDILMILDADLTMPPEDLPKFY
                     EAIASNKGDFINGCRLIYPMEKEAMRFLNMLGNKFFSQAFTWLLEQPFKDTLCGTKVI
                     FRDDYDRLVTNRKFFGDFDPFGDFDLIFGAYKLNLKIQELPIRYRERTYGSTNISRFK
                     HGIILLRMCFFAARKIKFV"
     gene            complement(14542..15129)
                     /locus_tag="PKX59_03110"
     CDS             complement(14542..15129)
                     /locus_tag="PKX59_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HOZ89887.1"
                     /translation="MNFLAHLYLSDNNEPLMVGNFIADHLKGNDWKSLPEGIMSGVKV
                     HRFIDHFTDTHPVVEQSKVLLRPYFHKYTPVISDIFYDHFLAAEWDKYSNQPIDEYAR
                     YVYQLMHKNYQFLPARTKEMLVYMENDNWLKNYATLNGMHRALSGMGRRAKFDNNMNQ
                     AAEFLYDNYELFEKHFKEFFPELESAVKKEISTQT"
     gene            15204..16076
                     /locus_tag="PKX59_03115"
     CDS             15204..16076
                     /locus_tag="PKX59_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HOZ89888.1"
                     /translation="MQVLQKTSQYLSLVKFSHTVFALPFAAIGFSLAISQNGLPVFWP
                     LAIKVLVCMITARNAAMAFNRWADRSIDALNPRTALREIPAGIIKANRALWFVIVNAL
                     LFTVTAYLINKLCFFLSPVALFVVLGYSFTKRFTALCHLVLGAGLALAPVGAYLAVTG
                     KFDITPILIGVAVLCWVSGFDIIYALQDEQFDRENKLHSIPSLMGKKQALQVSRILHF
                     LSAGFLALFLYQAHVGLIAWSGLAIFCALLIYQHSLVSENNLSKVNIAFATTNGIASI
                     VFAGMVISDIFLLH"
     gene            complement(16168..17904)
                     /locus_tag="PKX59_03120"
     CDS             complement(16168..17904)
                     /locus_tag="PKX59_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89889.1"
                     /translation="MIRTIQNTTKAAIILIMFSYIGNPFPAYAKGKTLKATLKGQNVS
                     CFGGSNGSISLDISGGKAPYQITWNDGTSGYAMLDKPAGEYSVSITDSKGSTVSQSIT
                     LTEPYAMTIYSQVTSEQCYARNGKANLLVTGGTQPYQYEWSNQTRQQNLEAVAAGDYE
                     IKVTDKNGCIKQSNITIDRNKVLELNIAKYSPLCNDEASGLIDVEVKGGWSPYTYEWS
                     NGAKTEDQSGLKAGNYSVIVKDNNGCTAKAEVQLKNPETIKVEAKVTEADLNKSNGSI
                     MLQVSGGTGNYTYLWSNMESSQSLNNVEEGVYAVRVDDAHRCSAAAYFTVNEKSPMQV
                     ESVVKHELCNGGKNGSIKLNVKGGVAPYKFQWSNGMNGATISNLTASDYSVLITDANN
                     KVYRNTFTVKQPEPIGIFATVLDESYTGKNDGSIKLQVIGGMPPLKYQWSNGQTVADL
                     ANLSQGTYTVRMKDGNGCAMEHHVLVGINYTDAMLRTAFANEDNAMMEIYPNPFSNNF
                     TMKFSTPVNDIQSIIVYTMDGKIAQQLNPDLKQQQVNEIAVNASKLKPGNYLVKVTTD
                     KKIFTRIVTRTM"
     gene            complement(18029..19198)
                     /locus_tag="PKX59_03125"
     CDS             complement(18029..19198)
                     /locus_tag="PKX59_03125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ89890.1"
                     /translation="MLRIEVFNSVREMPANDWQTINGENVIFTSFSYLSALEQSKPAG
                     MQFRYVIVYDSKMPVATLCFQLLDVANKDLDGVLNLKDKGWLLQNLNKSINRLLFKCQ
                     TGKVNFLICCGSFFVSGEYGINVINRDALPAVIQLLPEVRQKITDTISNASVCGWMVK
                     DFYNNTKVDQPLLEDDFFELPMDPEMILYIREQWHSFEEYLASLTTKYRVKANSVIRK
                     IEDVDVVALNAAEVLKYGDELIRLYLNIQQKATVQMARVNILYFEKLLTIMPEKFYVK
                     VFFKESKVIAFYCGFHHAGHHEAHFIGIDYSLNKTLSLYQNILYRFIEEAISQRSSNL
                     YYGRTAMEIKSTTGAKAYPLHAYFNLSNTVLNKMVKPVVRRFTPEEWIARNPFRE"
     gene            19327..20616
                     /locus_tag="PKX59_03130"
     CDS             19327..20616
                     /locus_tag="PKX59_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOZ89891.1"
                     /translation="MKYNSINSKLFVDNRKKFTAELKPKSIAIFQSNDEMPRSGDQNF
                     TFRQNADLFYLTGIDQEESILVIFPDSPLPQYKEILFVRKTNERIAIWEGHKYTKEEA
                     QATSGIKHIYWTEEFNAVVPMLMHHAENVYVNINENDRHATEVPYRDMRFAREWKNRF
                     PAHNFERLGQIMAKLRSRKHDIEVQLISEAIKHTESALRRVLKFMKPGVMEFEVEAEI
                     IHEFVRNRCNGHAYSPIIASGASACVLHYNENNKPCKDGDLVLMDFGAEYANYAADLT
                     RTIPVNGKFTPRQKEVYEAVLRVMKAATKLLVPGNFIEKYHAEVGKMMEEELVNLKLI
                     TKDEIKNQNPEWPAYKKYFMHGTSHFLGLDVHDVGNKYQAMQAGMVFTCEPGIYIPEE
                     SIGIRIENNILITDKAPVDLMATMPVEVSDIESLMRK"
     gene            20652..21920
                     /gene="glyA"
                     /locus_tag="PKX59_03135"
     CDS             20652..21920
                     /gene="glyA"
                     /locus_tag="PKX59_03135"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOZ89892.1"
                     /translation="MQKDKAVFKLIKEELARQRHGLELIASENFVSQQVMDAMGSCLT
                     NKYAEGLPGKRYYGGCEVVDEIEQIAIDRAKTLFNATWANVQPHSGAQANAAVMLACL
                     QPGDTILGFNLSHGGHLTHGSPVNFSGKLYRPTFYGVEENTGTIDYNKVEETALKEKP
                     KLIICGASAYSRDWDYVALRSIADKVNALLLADISHPSGLIARGLLNDPLPHCHFIST
                     TTHKTLRGPRGGLIMMGKDFENPWGLKTPKGETRMMTALMDSAVFPGTQGGPLEHVIA
                     AKAVAFGEALSDEYFEYCLQVIRNAKTMANAFVKKGYKVISGGTDNHLMLIDLRSKNI
                     TGKQAENALVRADITVNKNMVPFDTQSPFVTSGMRVGTPAMTSRGMKETEMARIVELI
                     DMVIMNHTNGAVIEEVKTTVNDMMEHFPLY"
     gene            21985..23043
                     /gene="porQ"
                     /locus_tag="PKX59_03140"
     CDS             21985..23043
                     /gene="porQ"
                     /locus_tag="PKX59_03140"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOZ89893.1"
                     /translation="MRITTLIAALLLPVCLKAQTGGSAIFRFLDLPSSARHAALGGNF
                     ISIRDNDLNTSFYNPAILNVDMDKQITFNYNPYFSGIKYGYTAYAHHIENIGTLSAGI
                     QYVSYGKFDRTDETATDLGTFSGGDYALNISMSRPVGIDSVLFVGASLKFIYSHIDTY
                     SAFGIAADLGVNYYSPDKLTSAALVIRNAGRQLKSYTEKNNEPLPIEIEAGITQKLAK
                     APFRFTLTYRHIEKWDLSYIDNNSQETDPLTGEVKEPEKASFGNKLLRHLIISNEILI
                     SKNFHLRMAYNFQRRNEMKVESRPAMVGISFGLGLKISKFHLSYGRSLYHLAGGTNSL
                     SISTYLNDFKRKPKTTDS"
     gene            23118..23804
                     /gene="cmk"
                     /locus_tag="PKX59_03145"
     CDS             23118..23804
                     /gene="cmk"
                     /locus_tag="PKX59_03145"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOZ89894.1"
                     /translation="MIIAIDGYSSCGKSTVAKAIAKRLKLNYIDSGAMYRAVTYYFLQ
                     NNIPFSKTADVIADYSSILENIQIEFIRNEQTGQSEIYLNGKNIEAEIRQMDVSDNVS
                     YVSAIKSVRKKLVAMQQQMSTLGNLVMDGRDIGTTVFPNADLKIFMTASPEVRVYRRY
                     HELIEKGIKVTPDEIRKNIELRDYEDTHREESPLRKPDDAIILDNTYMSRDEQVQFVL
                     DAVAKLKKAI"
     gene            complement(23823..25622)
                     /gene="argS"
                     /locus_tag="PKX59_03150"
     CDS             complement(23823..25622)
                     /gene="argS"
                     /locus_tag="PKX59_03150"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOZ89895.1"
                     /translation="MIFEQELASITNEALKVLFNFEPDESLLTYTQTRKEFDGDYTIV
                     VFPFVRQSKKSPEETGRLIGEYLIKHASFISNYNVIKGFLNITVSDEQWLKRIAVAFA
                     DSEYGFTKADKSKPVIVEYASPNTNKPIHLGHLRNILLGYSVSLLLKATGRNVKRVSI
                     INDRGVHICKSMLAWKKFGNDETPQSSGMKGDHLVGKYYVEFDKQYKQQVSEMISAGI
                     EKDKAEKNAPLMQEVRQMLIEWEQGNEEVTALWKKMNGWVYEGFDVTYNRLGVDFDKT
                     YYESETYLLGKKIVEEGLSKGVFYKKADNSVWVDLTNDGLDEKIVQRSDGTSVYITQD
                     LGTAVLRHDEFDFDSKIYVVGNEQDYHFKVLFLILKKLGYEWAEKLYHLSYAMVDLPS
                     GKMKSREGTVVDADDLMDEVTAAAAEETSKLGKVEEFSSDELKQLYETLGFGALKYFI
                     LKVDPEKRMLFNPAESVEIHGNTGPYIQYVHARICSVLKRASQMEQLSNYKNTAIESL
                     VSMNEKERELAINILRFKETVETAAEKFSPAIVANYVYELAKNFNQFYHDNSIISEGE
                     LETSQFRLCLADACAMTIKKSLNLLGIKAPERM"
     gene            complement(25634..26515)
                     /locus_tag="PKX59_03155"
     CDS             complement(25634..26515)
                     /locus_tag="PKX59_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HOZ89896.1"
                     /translation="MTIIDGKQTAEEYKSILKQEVKALVATGKRPPGLAILLIGNNGA
                     SETYVANKLKTCHDVGFNASLFRFENTVSENEILNCINELNFNSSIDGVIVQSPFPNH
                     ISPQKINEAIHPAKDVDGFHPMNAGRLQQNKPCYIAATPKGIMQLIEKYKIETQGKHC
                     VVVGRSNIVGMPMSILLSRNSKPGNCTVTICHSKTVDLAAYTKRADILIAAIGSPELI
                     TADMVKEGAVVIDVGITRVADKATKSGYRLKGDIKYDEVAPKCSFITPVPGGVGPMTI
                     IGLLQNTLLAAKNAIYN"
     gene            complement(26546..27877)
                     /gene="ffh"
                     /locus_tag="PKX59_03160"
     CDS             complement(26546..27877)
                     /gene="ffh"
                     /locus_tag="PKX59_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOZ89897.1"
                     /translation="MFENLSDKLEKAFKNLKGQGKITEINVAETLKEVRRALLDADVN
                     YKVAKQFTDTIKEKALGQNVLTAVSPGQLLVKIAHDELATLMGGQMTDIQLKGNPSVI
                     LMSGLQGSGKTTLSGKLANFLKTKKGKTPLLVACDVYRPAAIDQLKVLGEQIGVEVYS
                     EVENKNPVEIARHAIKQAKQNGNNVVVVDTAGRLAIDEQMMNEIAALKNAIEPDEILF
                     VVDSMTGQDAVNTAKAFNDRLNFDGVVLTKLDGDTRGGAALSIRSVVNKPIKFVGTGE
                     KMDALDVFHPERMADRILGMGDIVTLVEKAQEQFDEKQAAELQKKFAKNQFNFNDFLS
                     QLQQIKKMGNIKDLVSMIPGVGKAVKDMDIDNNAFKSIEAIIQSMTPAERANPDLING
                     NRKKRIAAGSGTGIPEVNKLIKQFEDMRKMMKMMTTGNMKNMMRNMPGMRR"
     gene            28090..29508
                     /locus_tag="PKX59_03165"
     CDS             28090..29508
                     /locus_tag="PKX59_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOZ89898.1"
                     /translation="MKPFKSIFLFILFFSEANAQKIWSLKDCIDYALNNNISVQQSII
                     ASKIAAATYQQSVGSLFPSINASSSLNYNYGRSLDPTSYQYVDQTFQSGGASLNGNLP
                     IFQGLELQHNLRKSSIDFDVSKLDVRKVQNDIALSVAAAYLQILYSNEQLKATNDRIE
                     AVTKERNRAKILVDNDLLAEGSLLDADALLATEEFNKASAENLLTSSKLTLIQLMQLE
                     GANDIAIETPALEIPSQESLSLSSESVYNEALKNLPEVKASELRLKSAKESLGIARAA
                     RYPRLSAFGSLSTNYSNQATSLDGNPVFSGYVPSGAVTSSGEQVLQPVFNYKYIDTPV
                     KDQLNNNFGKAIGFNLSIPLFNGWATNSSIKRSKLSFENARLNALQTKNTVFKNVQQA
                     HADALAALNKYAAANKSVTAQEKAFLYTEKKYNAGLLNSIDYVNARNNYTKSQSDLIQ
                     SKFDLILKIKVLDYYLGKPLSF"
     gene            29518..30894
                     /locus_tag="PKX59_03170"
     CDS             29518..30894
                     /locus_tag="PKX59_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ89899.1"
                     /translation="MSKKNNTLRNIIIIAGVVIVALVIAKKAGWLGEENVTNVSTEKA
                     SLQTITESVSASGKVQPEVEVKISSDVSGEIVEMLVKEGDVVKKGQLLCKINPEIYIS
                     SLDRAVAGLNSTKANYESSKSRLSQAESQLVKAEIAYTRNKKLFEEKVISASDFEAIK
                     SAYEVAKAEVDASKQGVSGAQYNINSGSATVREAKENLNKTEIFAPVNGTISKISKKK
                     GERIAGTSFTDGTEILRISNLQEMEVKADVGENDIIRIHLNDTTLVEIDAYSPRKFKG
                     IVTEIANSPNESLTSTEQVTNFAVKIRILRESYSDLIPSDHPEYSPFRPGMSATVEIQ
                     TKKVSNVVSVPIKAVTTRDTTIRTSDKAVVRKNDDTPPDDKVVVSSNTNDTPVKEIVF
                     VNDNGIAKMHVVKTGIQDNNFIEILEGIKVGDEVITDPYQAVSKKLVDGDKIKVVPQN
                     QLFKEEQK"
     gene            30900..31565
                     /gene="tsaB"
                     /locus_tag="PKX59_03175"
     CDS             30900..31565
                     /gene="tsaB"
                     /locus_tag="PKX59_03175"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOZ89900.1"
                     /translation="MALLLHLETSTDTCSVCISDNETIIDCEETHNSKSHASLLAVFI
                     DELLKRNRLTYKNIDAVVVSCGPGSYTGLRIGVATAKGLCFALKIPLISVATLQSMAN
                     IANKQCKGQLLCPMIDARRMEVFTALLNPQLDFIEKENAVIIDEQFHHDKLVKNKICF
                     FGNGAAKLPDSIKINSNCVIFDEFKMSAMGLVTLAFKKFTQQTFENLAYFEPFYLKDF
                     VGK"
     gene            31950..36191
                     /locus_tag="PKX59_03180"
     CDS             31950..36191
                     /locus_tag="PKX59_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89901.1"
                     /translation="MKDFQKIISFFMVTLIFVLSSYRSEALIFQSVASGNFNTLIWNQ
                     AGLYPTTGDTVVINTGTTVTINGFNTDVLGSVTINSGGNLTIANNAVAQLVFAGDLVV
                     SGTLINNGRINMTVPGNFVLGNGAVYDHNPKINIAVDENVFENGVENFSPTSTLIIRK
                     WASTALPLAGPTRVTSDIGNLVTSVTGTTWEQRGQFSPNRIKGSLLVTDGVLRMDDGS
                     GATTVLTLNDVTLRGNSAIIFQEGNNRNLTLTMGNFTDSSVLVNQPTIIMNMSLGTLN
                     LTVNGNFITRHDFIAVFDSTFPIRSASVTANIMGDLLIQGSGNKFDFCKQVNAPLTLT
                     VGGNTKIANNPNYVRFVDSGNGFLNYTTGDLNISAGLKNIFLGGNDLLDASNNPLYPL
                     PTGYATVNVLNDFNLSGVTNTTILLSPGNVARTKLIVGHDLNSSSASVNFVVANNIGI
                     VNIEIGSNLFLNGGSMNVQANASSNAIDSIAVTRSFIFNSTNPANFFRGNSGAGNTFI
                     RVLGDFNLMNSGTAANQGVCGTYLNNGNLEFYVAGNFNQSAGAFYGIQDGTGRNTFTV
                     DGTLNQSGGFFKGTHATNIFTAGKSIFTLGGFSFSGGNYIQYNAANDSVICTVNGDFN
                     VNYTAVTNLVSFIPYLHPVYSNTSPLKLTVTGDMYFGGANGTFYSSVTQGNELISIGA
                     NLTFDNGNNSFNNYPSAVITTTHNVNITVGGNLTCNNGINNFSAGRGDLAGTINGDLI
                     ISGGTLSLKSNSAYNPIIINVLGGFNMTNGNFFYTNNATIPPITTTVTINSDDDNTGD
                     FVHSGGTINFVNNTLNPTVQSLTIKSPNVLLGPSGTIKRNGAGTIASRGALDFARTGT
                     TLFTRLNGHTVDHTVLTVVNGTTLEVISGDVQLTSYVEEFAGQTLPMLYVSSGATFTL
                     RNNSSVYTTGNFLKSTVQGASGSRIRIQHTNGFYSGFSSAAINSSGNMGFLLSSSSTV
                     EYFGDDNQIITGIGMGNAVGGNQKYGVLEINFGGDPNTEYVYPTSNGTVNVRNQLKLT
                     AGQLRLDDDNDPTNGGRTIIIENSPNTSITRVAGYIRAETYDGASNVKWMFAADNTPH
                     IFPFGLDPTTYIPLTVTSTGGNAGFITASTFKTSPANLPYPPGVGHVNSVATGADNSA
                     DCVDRFWKINQSGTSTTFNAVFSAPVAEFPAIAGPYKAQHYNYVGGFWDNPFQGAQAY
                     ASAGPIRTVTATGVSVTNDWWVLVNQNTPLPVELLSFDANCVKNHHQIKWTTASELNN
                     DFFTLEKSADGTEFTLFRKIEGSGTVNDSKNYEVSTDDGIAAYYRLRQYDFNGTMHDL
                     KTIYAPSCQNDEFKLLSVNQVEDIVNSIVSSPDNIKAEITLFDLKGALIFKEEVSLST
                     GVNSVRLPINLAKGLYYFQIINDNQVTQGRKFLVN"
     gene            36288..37835
                     /locus_tag="PKX59_03185"
     CDS             36288..37835
                     /locus_tag="PKX59_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOZ89902.1"
                     /translation="MTLIGRIIKASIDLRGKIEIVPFSPFISQKRVLKKLIRKAQFTM
                     FGEAYNFQGMLGNKNFVTKFRAAVPVHDYNSIFSNWWNKSLHDQENVCWPGKVRYFAL
                     SSGTSGSSSKHIPVTKDMSKAIQKASLKQILSLSKYDLPFSTFEKGILMLGGSTHLHQ
                     QGSYFEGDLSGITASQIPFWFQHFYKPGRKISREKDWHAKLDEITHKAKDWDIGIIVG
                     VPAWVQLLIEKILDHYKLKNIHELWPNLEIYVHGGVSFEPYKKGFSKLLGKQIYYIET
                     YLASEGFIAYQDEPNTRGMRLLVNNGIFYEFIPFNEDNFDADGELKHKPKTLLLNEVK
                     ANEPYAILLTTCSGAWRYLIGDVITFTNLEKCEIQIIGRTKHYLSLCGEHLSVDNMNR
                     AIELVSDELNIDIPEFGVAGIPYQSLFAHKWYIGCDTYNDSENLKKLIDEKLKILNDD
                     YRVERRSALKEIFVEIIPTSWFYKWMELQGKIGAQNKFPRVMKNKVFEDWEKFVASQH
                     KNINQAV"
     gene            37855..38490
                     /locus_tag="PKX59_03190"
     CDS             37855..38490
                     /locus_tag="PKX59_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOZ89903.1"
                     /translation="MIEPVISGFLLGCFLAILAGPVFFLIINISLRKGFSYASRVAIG
                     VLISDTLFIFIAYFGSNFILLLNNHRFIAGIISGILLIGFGIALILKRNSIPAEAITV
                     NEKTDSKSLYILKGFMINSMNPSVLFFWIGVSGTVSVKEHYTIHHLLLFYGSILITLF
                     SFDLFKAYIAHKLKSILTINLLTWLNRITGMALLGIGLYTLIVACWQAYAK"
     gene            complement(38544..38918)
                     /gene="crcB"
                     /locus_tag="PKX59_03195"
     CDS             complement(38544..38918)
                     /gene="crcB"
                     /locus_tag="PKX59_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOZ89904.1"
                     /translation="MRTILTIGFGSFIGGMIRYMLSLFIQSKTVSEFPVGTLLVNIIG
                     CFLIGVVYSFAEQGKMAVEWRLFIATGILGGFTTFSAFSMESFMMLRNGYYGMMTVYV
                     FLSLAIGISATIGGFLLVRTLN"
     gene            complement(38954..39517)
                     /locus_tag="PKX59_03200"
     CDS             complement(38954..39517)
                     /locus_tag="PKX59_03200"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOZ89905.1"
                     /translation="MIEQIKEFDTNLFLWLNSIHSPAMDSFFFAITNRFVWIPVYLIL
                     LFLLIKKKKNKTWSILLFIVILIICSDQLSVLIKENVMRYRPCHNLNIQGVVHTVNNK
                     CGGKYGFVSSHATNTMALFAFLVLSVFKKERIVIALLAIYVLLVSYSRVYLGVHYPFD
                     ILGGWITGLAVALVLYYFVYNKLFNQP"
     gene            complement(39514..40524)
                     /gene="obgE"
                     /locus_tag="PKX59_03205"
     CDS             complement(39514..40524)
                     /gene="obgE"
                     /locus_tag="PKX59_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOZ89906.1"
                     /translation="MEKNFVDYVKICCRSGAGGPGYVHFHRDKKTAKGGPDGGDGGRG
                     GHIIVRGNKQLWTLLHLKYRKHILADAGQKGGNALRTGADGKDEILEVPIGTIARDFE
                     TNKFLFEIEEDGQEVILARGGRGGKGNAFFKTSTLQTPRHAQPGGEGIEEWKILELKL
                     MADVGLVGFPNAGKSTLLSVVSAAKPEIADYPFTTLVPNLGMVYYHDDRSFVMADIPG
                     IIEGAHTGKGLGLRFLRHIERNSILLFLIAADAKSIKEEYKILKNELRQHNPELLDKR
                     RVIAITKSDMLDEELLKELKKEVPNDKNPGGGKVPVVFISSVANFNIDKLKDVLWKEL
                     NS"
     gene            complement(40555..41748)
                     /locus_tag="PKX59_03210"
     CDS             complement(40555..41748)
                     /locus_tag="PKX59_03210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOZ89907.1"
                     /translation="MENVCITGVGLVTAVGNNANETILAIQSGKTGIGIPKYVKGRYA
                     SMIPFGEVKLSDMQLADELSIRNKVVSRTQLLAHKVFNEALNNSALHIDDLKNTETSI
                     VVSSTVGGMYLTDELYADSNNLSKGSDYLSLYDCASIGIYLQQLTGAKGVINTINTAC
                     SSGANALIYGMRLLKNKRAKRVIVGGVDCLSKFTINGFNALNILSTRSCRPFDNNRDG
                     LNLGEGAGFLILESSDTAKEEKVLAYIKGYANSNDAFHPSALSDEGFGPMAAMRGALE
                     SAGLMPKEINSVIAHGTGTPNNDRVEGLSIKRIFGESLPPVCSLKSILGHTLGAASAV
                     ESVLAVIGVRDGHVYPSRNFETPDEETGLVPNLNFKKEKIKHSLLNSFGFGGNCSSLI
                     FSSVN"
     gene            complement(41760..42029)
                     /locus_tag="PKX59_03215"
     CDS             complement(41760..42029)
                     /locus_tag="PKX59_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOZ89908.1"
                     /translation="MTKEEIKPELKKHIIKYLNLLDLTPESIKDDQPLFGEGGLGLDS
                     IDSIELIVLLEREYGIKITDPKEGRKILIDVNTIADYIIQHKSAK"
     gene            complement(42026..43297)
                     /locus_tag="PKX59_03220"
     CDS             complement(42026..43297)
                     /locus_tag="PKX59_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ89909.1"
                     /translation="MSPLVASIIKEFKVLSRDKTGLLLMFLMPLVLALLMTVLQNSAF
                     KIVNENKVDLMLINNDSNSLFFQNFCKGIEQTGMFDLKINTKEISSKKLSEDKESIVV
                     VTIPKGYAYTLDQEVKAKAEQSLQMFGMLESVSTKGIIGVKNIELSFDPVLQESYRFS
                     IKSTVLSVVMAIQNEMLLKQLFYDVNQSEPDSAALSKILPKPLLLSESLIKENSVANP
                     NATQHNIPAWTVFAMFFTVISLGGNIVREKRSGSFLRIKTLPISITQMLIAKQVVFWI
                     VTVLQTLVLFSIGILIFPMLQLPPLVISSGLLPLFLATLCCGLCAVSFALCIGVYSDT
                     VEQANGFGAASVVILAAVGGIFVPLFAMPEVFKLIANVSPFSWAMKAYYSAILEISPI
                     HKLLLNVLPLLLLTAILQLIAFIGLKRKNLL"
     gene            complement(43297..44037)
                     /locus_tag="PKX59_03225"
     CDS             complement(43297..44037)
                     /locus_tag="PKX59_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ89910.1"
                     /translation="MDVLTVSSLSYTYKNSDHAVLSNINLHVGKGEVFGVFGPNGAGK
                     TTLLSLVTGILTCRLGVISILGKDASTQRKNVNQIIGYVPQDFAFYEELSAIENLIFF
                     GAMYGLKEQALDNRAKELLEEFGLMNVAERYVKEFSGGMKRRLNIAIGLLHKPQLLIL
                     DEPTVGVDVQSRLAIMSYLKKINSEGTTIIYTSHLLKEAEEFCSRIALIDKGKVIAID
                     TIANLLIQNQCKSLEEVFIKLTGNNYRD"
     gene            complement(44051..45058)
                     /locus_tag="PKX59_03230"
     CDS             complement(44051..45058)
                     /locus_tag="PKX59_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HOZ89911.1"
                     /translation="MQINFNHQQSAHCENGVTVNLLNYSGLRLTEPLVFGMGSGLFFI
                     YIPFLKVNNAPAFSYRTMPGLIFKRTCSRLGIKVIRKKFRSESEAQRIRDDCVIKNQP
                     VGCQVGVYYLTYFPKEYRFHFNAHNLVVYGKTDDRYLISDPVMEDTTSLTEFDLNRVR
                     FAKGPLAPKGQLYYPQIENIVSDEKIVYAIKRGIKQNIRDMLYIPGPIAGVSGIKYVA
                     KKILKWHDRLGAKTTGLYLAQLVRMQEEIGTGGGGFRYIYAAFLQQSHAYLKNDSIIK
                     IAEQFTKAGDMWRDSAIQSAGIFKGRLTDRADFVNMHDRLMQIAEVEKNAFTDLSKVK
                     L"
     assembly_gap    45160..45188
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<45189..45468)
                     /locus_tag="PKX59_03235"
     CDS             complement(<45189..45468)
                     /locus_tag="PKX59_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89912.1"
                     /translation="MNKKDIPQDHSALVNYIKELCYAVGEDGQYTTANSTGWDVKTNA
                     LNLAWQDIEQRIVTAYSQVKNREVSPILYYMELRLMDIGILSAYTGFWK"
     gene            complement(45468..46613)
                     /locus_tag="PKX59_03240"
     CDS             complement(45468..46613)
                     /locus_tag="PKX59_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOZ89913.1"
                     /translation="MLHEVYITRLSRFLPNKPVLNDEMEQYIGLINEHSAKSKRIVLR
                     NNGIKCRYYALDKNGKATHTNSEMTALAIKELFANRQEEIKEVDLLSCGTSSPDQWMP
                     SHAVMVHGWLTEAKDMEVVSPTGNCCSGMHALKYIWLAVKAGQSKKAVVAGSERTSRI
                     MHHQSFEEEAKKLSQIEENPYISFDKEFLRWMLSDGAAAALIENKKKENGLSLRIDAI
                     EGVSYAHLVEPCMYSAADKMDDGSFVSYMDMSPSEIVTNSVLNIKQDVKLLSKYIVDL
                     GTDKLISMLKAHNLPGDKIDWFLPHISSEFFRDKMDEGFRSKGLVIPMNRWFTNLSEV
                     GNVGAGSIYLMLEELMRTNRLKQGEKIVLAVPESARFSYVFAILTVV"
BASE COUNT        15093 a         8879 c         7849 g        14915 t
ORIGIN      
        1 aggatttgta tctaattgat ttgtagtgtg gaaatttgac gttagtcaga atgctgtgag
       61 accccgaaac aacctgcgtc tttttcaaac tttgaacgtc ttacgtttgg acaattaatt
      121 ttacagacag aatatgacaa cagacgttac taatatttgg acaaatttta ataaagagct
      181 caaaggcttt atctacaaca aaacaagaaa ttcggctgac acagacgaca ttttgcagga
      241 agtttttgta aaaatcattc gcaatattga aaaagtaaat caatccgaaa acttgcgaca
      301 atatctttat ggaattgttc gtaatacatt aaatgactat ttcaagaatc aaaagacatt
      361 ttccagcgac accgaaattc aagaacaatt ttcagaaaca gaaactaatt cattaaacga
      421 aaccattgca gaatgttgca taaaaccctt tatcaacaaa ctgcctgaca actacaaaca
      481 ggcactttta attacagagt ttcaagacat ttctcaaaaa gaattagcca aaaaacttaa
      541 tatttcatat tcaggtgcaa aatcaagagt gcaacgtgga aaggataaac ttaaagaact
      601 tattttaaat tgttgtgcgt atcaaaacga caaatacgga aatttgattg acaccgaaga
      661 taaaagctgt ggttgctcat aatttttgcg tctattttct tgtattcaag tgttactatc
      721 aaatggcgat gttgccataa attaaaagta aaaaaatgaa tacagaagta atcaaagaag
      781 aaattaaaaa atcctatggc gatgtagcaa aaggcaatat tcaagtaggt tgttgctcaa
      841 cagaaacagg ttgttgcacc acaagcgaat ttagtaactc aatgagtgaa gattacagca
      901 atgtagaagg atacgtaaaa agtgctgacc ttgctttagg ttgtgggctt ccaacagaaa
      961 ttgcaaacat tcacgaaggc gacacagtta ttgaccttgg ttcaggtgca ggaaacgatg
     1021 cttttattgc tcgtagaatt gttggcgaaa caggaaaagt gattggtatt gatatgacac
     1081 ctgaaatggt aattcgtgcc ttacaaaata cccaaaagtt aggttataaa aatgttgagt
     1141 tcgtattggg tgaaattgaa aatatgaaag aaatttcaaa caatgttgca gacgtagtaa
     1201 ttagcaactg tgttatgaat cttgtgccta acaaagagaa agcatttaat gaagtaaaca
     1261 gagttttaaa agttggtgga cattttagca tttccgacat tgtttttcaa ggtgcaattc
     1321 cacaaggtgt tttagaagca tctgaaatgt atgcaggttg tgtagcaggt gcaagtgaaa
     1381 aaggacaata tttaggaata attaaaaaag caggatttaa aaatatttct ataaaaaaag
     1441 aacgacttct tgaattgcct gacgaacttt tattaaagta tctaaaccaa caagaacttg
     1501 acgattacaa aaaatcgggt tcaggcattt acagcataac agtttatgca gacaaattag
     1561 aagaaggaac ttgttgcgac acgacagaga atggctgctg tggaagtgaa acaaaaacag
     1621 aaaaaacaac agaaaaaact gcttgttgtg ggacaacttc aaacggaaat tcaagttgct
     1681 gtggataaaa agcactgcca ctaacaaggg ttcctatgta aatcccccta ctcaggtacg
     1741 gagataaatt ttcttttgaa gaggaaagca attttgaatt ttgccttcct gcttcgcaag
     1801 agttttttat ccgttttcaa atgtaatcta ttttcctatt ccattgtttt tttccctctc
     1861 ttttttgttc ttcaaactca gtctcctatt tgtagcttgt gtcctatatg gcacaactac
     1921 cagcacctgt gtggttgtag agaactataa atacagctac ctgcttggcg atgtagattc
     1981 cttttggaac taccacccat gttttgtagt ctgtaaatct cttttgttgt tgcgagtttt
     2041 aaagatgttt tttactgttt ttctttttct tctttcaatt tatttattgt gttatttact
     2101 ctgtatggtt gcccgttctt tattacagac agcatacgct ttaccatttt atgtgcaatt
     2161 tttacaatta cactcttcgg attctttgat gcatgtttgc ggtaatatgc ctggatttcc
     2221 tgatcgtaac gtaatgctac ccatgcactc tctattatgt aagtccgcaa tagcgacttt
     2281 gatcgtggtg tcactccaag catggtttcg ttgccaccgc tactgtaaat accgggaacc
     2341 acacctatat atgaactgaa ttgtttttcg ttggcaaatc tgcgcaagtc gccacactcg
     2401 gctattactg ctgaagctaa aaatcctcca attcccggaa tacttcttaa aagattataa
     2461 tcccttgtgt aatgtttctt gcagtaagct ctaagagtgg tacctatgtt aagatactcc
     2521 tgatgatgaa aggctaaact ccgtagtttg ctgtcaagac tcatctgtgc gcatgcgttt
     2581 taccagttta ggtttttaag ccatttaata aatgctttgc tccagttgtt attgtcaaag
     2641 tctatgggta tggttatgcc atgaaacaac aacatacttt ttatatgtgt ctttactttg
     2701 cgaagttgtt ttactatctg attacgatga cgcaccaaac tgcgtagttc ctcttcctct
     2761 gatgtgggga tatagatgcc gcgaagattg ttgttataaa gttgcttgca caaattgcgg
     2821 ctgtctattt tatctgtctt ctgataattt tgtttatcca tgcgtggcac atcggctggg
     2881 tttactacca gtactttcca gcccatgcct atgaaatgac gggctgcact aaagccacag
     2941 catccggctt cgtaggctat ggacacctca tgacccggaa aattagaact tatgtagtca
     3001 tacaaaccat ctgcatcagc aggcatattt aatgtgcggt ggtcgcatat atcggtgcgg
     3061 atacttactg accaactttt cttgtgaatg tccatgccaa tgattacttt tggtgtggat
     3121 gatgtttgtt gtgtttgcat tttaatttct atttaaaatt ttaatttatg ttttgcccct
     3181 tcgaagttag gattttacat agatgctttg cgtcaggcgg gctgaccttc aaacttgtag
     3241 ctttatgctt tgaataaacg gtagtaataa attgaatctt tgtgctcttt cgtactttca
     3301 aagtctctcg catttcagag gactttgaaa taataataaa acattaaagg gtaaatacgt
     3361 ttttaataat aatagaacac atctaatttc accaatcatg tcgcacggcc tctgacagcg
     3421 taatttaaca caaacgctaa atttcaatac agcatagttt gctgcaattg tcttattaaa
     3481 tcattttctt tttacttttg agctaatgat tagttttaat tttgatggtt ttcggaacaa
     3541 taacaatcag aacattggct atatgacagg aatttatttt aagttacata caacccgttt
     3601 cgtgatcttt tcaaaaccaa caattaaaac tgaaatgatt aaatacaatg aaaataatta
     3661 acacacttaa aaacaaacaa ctatgctacg aatatatctc tctgcattct ttataatatt
     3721 ttttctaggc tggattctat accgactttt tattaaaaaa gacttaaatc aaaatataaa
     3781 tatattttat ctcggattaa cttttacagg aatatgggtt ttagtctatt atctattgct
     3841 aaaataaaaa ctaaaaaaag ctacttctaa aacaagtttt gcaaattaca gtctgacgtg
     3901 caaacttgga gcattgtgtt ccgaatgatc actaatatta aaatgttcct gataacaatc
     3961 ggtaaagtaa tctttaatct gtatgaaagt caaatccaaa aattgttata cgccatttta
     4021 ggtcaaaatg tcaaccacaa ctaacaataa taaaacaaaa gacccgttaa aaaatatatg
     4081 ccgaaaggaa caaggaaaac aaagtttgtt aaacatactc tgctgacatt tatatatttt
     4141 gcattaacat caaatagttt tggttgcagt ggctataaga ttacgcttgg gaataaaact
     4201 atattaggca gtaatgaaga tgcgtggaga cttacaccac aaatttggtt tgaaaaaggt
     4261 aaaggcaaca gtcaatatgg tgcagcattt acaggttcaa gatatgacgg agaaaatggt
     4321 tatgcaccac aatccggaat gaatgaaatg ggtttatcct ttgaaaggct tgcatcttac
     4381 cacccgattc aagaaaagtt tacaaacaga aaggctataa ccaaccctac aaagtattta
     4441 aaggacattc tgcatacttg taaatcagta gaagaagtaa aagagtttat caataaatat
     4501 gactacagtt attttattga agatgtattt ttttatgttg acaagtccgg aaaatatctt
     4561 atagttgaac cttatacttt gacaatagga aacgaaccaa cttttgttat ctcaaatttt
     4621 tgtccttcaa taacgtctga acaaactgca aataaactta caaggtacag aaacggagta
     4681 gcattcttaa aaaacggttt tgacactaca atagcattct gcacggcctt gtcagataca
     4741 atgcatgtat gtagaaaaaa aattggtgat ggaactttac tttcttccat ttgggattta
     4801 aacagtggga ctgtgaactt gtatttttat cacaactaca atgctactgt tcaattcaag
     4861 ttaagtgatg aattaagaaa aggcgaccac attattgcaa tagaaacact attcccgcat
     4921 agtgctgaat ttgaaaaatt acgtaatttc aaaaccccca aagacagtaa ttttatggga
     4981 ttatttattg ttgcttctgc cggatttttt ttactgactt ctatcttttt tcttactcaa
     5041 tttttcagaa gaaaagaaag taataagtac acttacattc agctcttaat gtttccaatt
     5101 ggcttaatca tgttttatta tatgtgcgta ttatgtggca gtgttaatgt attttacttt
     5161 cctgcaccat ataaagaccc ggctaatatt tttgttagca tgacttccta cataccattt
     5221 ctaatattac ttcttatatt accgttttct gttgttaatt taagaatttt aaaggataat
     5281 agttggagtt ttgtggccaa aggccttttt acatttaaca atatcattta ctttgttctc
     5341 attggattat ttacatattg gagattttac agcattttta attaaacaga aacgatgcaa
     5401 atacgactta tgatgaagta tctaatgtaa attcctcttc atgagtattt cacctcattc
     5461 gcacctttaa aattcgttaa tttatttatc ctctaaatct ctaacttcaa attttcaaat
     5521 gatcaaatca tcaaatcatc aaatcatcta ttatccccct atcgccttag ccttcgcaat
     5581 atactgttca ccctcatctt tttttcctgt cagcactaag gttacaccca aattatttaa
     5641 tgctcttgca ttcgaaggat tcagttcaat tgcctctctg aaataaatta ttgcattctc
     5701 atagtcctgc atggcagcat aactgtttcc agtattattt actgcatcct catttgtggg
     5761 gttaatctcc aaagcctttt tataattctg aattgcttta acgaaattat ttctgtgagc
     5821 aaaaacattt cccaaattat aataggctcc gtcagaaaga gaatctaatt caattgcctt
     5881 cattaaagag gtttcggctt tatcagactc ttgcatctca tcataaaaca cacccaaatt
     5941 aagatatgct tcttttgctg cagggtcaag atcaatggca tggttcaaag ctttaatact
     6001 ctcctgataa cgttttgctt ttccacatgc aacaccccat gaaataaaaa actgcgggtc
     6061 ggtaaatgtt ttactcttag ctgcatactc aaagctctca atggccttag cataattttc
     6121 tcgctcctta tatgccaaac caagatgata ataagcctct gaataagttg gcaaaatagc
     6181 tacacctttt tccaattgct caatactttt atccagaaga ttcatctttt ttgaataatc
     6241 cttttctttc aaagcctgtt caataagaat ggcactgccg taggcatatc ttatccgagc
     6301 actttcgggg cacgtcttta catcagttga cagcaaggtc agattgtttt tccattttgc
     6361 atttcttgca actgtcattc cgctataaac aacaaaaaca attaatataa gtccgaaaaa
     6421 tattctattt gttgctataa tcttattgat atcaattttc tctctgacaa cattactgac
     6481 tttaaataaa gtaaaaagaa cagaggtcaa tgcaatgcaa atgcctaatg aaggcaggta
     6541 ggtaaaacgc tctgccatta cagattctat caagaaaaat acattgctta caatactcat
     6601 ggtaagcaga aaataaaaaa taccaaaaga aaatctgctg cgcgacttaa atcctttaat
     6661 agccataatc agcaaaaaga cataaactgt caatgacaac aatgccttta tttcagaaaa
     6721 atttacaaca ggaaaagtgt tgtaagaata atcaaatgac agtggataag gaatgatgag
     6781 taaatacaaa tacttaccgg caatcataac agcagttgca aattgctgta tatagttttg
     6841 cgcattgaca attgaattgt tgatgggtaa tatttcatta aagttagaga ttcctttaag
     6901 aacaacaaag cgcaatgaca gataggctac aatagcaatt gccggtgacc atgacaattt
     6961 aatactgtta ccgattgtct tctgtctgaa aaaatatgca atcaatggaa atatcgctat
     7021 tgcagtgact gaactttctt tcgacaggag tgataaaata aaataaaaca aaactcctaa
     7081 agcataaaac ggttttgccc tatccaaaaa cttcaaaaaa ctgttgaatg aaagaatcag
     7141 aaataagaag catagaattt cgtctcggct tttaatatta gctacaacct ctgtgtgaat
     7201 gggatgaatg acataaatga gtgttgcgat gaaagcaaac agccagtcat ctttaaaaag
     7261 attaaccaag aaacgaaaca atacccatgc tgtgagcaca tagagcaata catttaccca
     7321 atgaccaata gaaggatttt ctggagctat ctgccattca acagcaaaca ttgctacaga
     7381 taacgggcga tacaaacttt cattcctgtc ccaaaagcct ttgcgatatg cagttgtaaa
     7441 aatttcggga atagcactta ctcccttctt tacaatttta ttgttttgca tcactgtgcc
     7501 atcatcaaca gtataatcat ggttgagtgt attggcataa agtaaaaatg ctattgcagc
     7561 aagcaacaat ccaagatatt tctttaactg tgttaaatta attcctgact gtacttttgc
     7621 ctgactttcc tgttttgtaa ccgttgcctt ttttttcatc ctatgttgtt tgaaacaaag
     7681 taaagtgaaa ttacaaaaaa tacagcacta cttacaaagc atttttcaac ctattcaatg
     7741 ttcattctga taaaccatga acgtgggtag ttaacattaa ctcgccctgc tattttcttt
     7801 aaaaacatca atggtatgtt ctgatatgtt attaaatgct ggcctattgg caacgaatcc
     7861 atcaaacgtg tttcaccttt gagtagtttt aacatggtag atttatcaac ttctgcttta
     7921 ttgaaatttt cctgatttaa cgctgttgac aaggcaagtt caaatgacgg caacaaattt
     7981 ttacctttta cttcaaatgc tgctgtacct gcatgtatta ccggcatatt taacagctga
     8041 cctaatgcat tttttaaaca tataggcata aatcttatat tgttatcatg tataaaatag
     8101 aacatctcct ctcccttgtc aatatgtttt ttcgcaaagt ctgcctgtac tatatcggcc
     8161 aacttcagat tcttagcaac aactctttca gaatttagat cattcaaatc aggttttctt
     8221 aacactgcta acgaaaatat ttctgttcct gttttatgtg gaaagaaacg atacataaca
     8281 gcatcttgtc tttgaaattc ttcaactccc ggaaaatctt taattttgag ttttacacat
     8341 tcagcattat gcttatttat aaattgctga acattggttt cattctcctg caagtttgat
     8401 gtgcaggttg aatatataaa ataacctccg ggctttaatg catcccataa atcatttaca
     8461 atacgttgtt ggcgtaaagc acaatgttca acattttgca gactccattc acttatagcg
     8521 gcttcatctt tcctgaacag cccttctccc gagcaaggag catcgcaaac aataacatca
     8581 aaaatatttt ccaaacgttt aaaaagtacc gcatcaccac gtgtgataat caaattagaa
     8641 tagccccacc gaatagcatt ttctacaaga acaggtgtac gtgaagcaat aacttcattt
     8701 gctacaacaa gcgactcctg atgaagtaat gataataaat gggtcgtctt tcctcccggt
     8761 gcagcacagg catcaagaac attaattggt ttttcattga ggctaagttg aaaaattacc
     8821 tgctccaaaa acatgcttga agcctcttga acatagtatg cccctgcatg aaacaatggg
     8881 tcgagagtaa aaacaggtcg ctgctccaaa aaatatcctt gtctgcacca aggaaccgaa
     8941 cttcctgaaa aatgtgtagt atgcttttgg ggatttatcc tcactgacac cggcacaggt
     9001 ttttgcaatg actgcacaaa taactcttta tctgattcag aaagaggcag cgactccaga
     9061 aattcttttg gcaacgacaa gaatatgata taacaggttg tggttgtaaa aatacatgaa
     9121 actgcacagt tttatatcac tctgacttac agtcatattc tgaatttctt agagcctcct
     9181 cctatgcttc atttattatc tttgccgtgt tttttaaaaa tatggacaga aattcgattt
     9241 taggagtagt tttaattatg gctgtgttga ttggcttttc aatattcaat cagccttcaa
     9301 aagaagagca ggaagcagca aaacgaaagc aagactctat tgcattggtt gaaaagaagc
     9361 atcaggacag tttgaaactt atcaccaaac ccgtagctgc actgaaagct gacaccgtag
     9421 cagttgccga cacgctaaac aacgactcac tatcacaaac aaaactaaca gataattttg
     9481 gagcatttgc attagctgcg caaggtgaaa gcaaaagtat tactttggaa agtgatttgc
     9541 tgaaattaaa gcttaacagc aagggcggaa agattgatta tgttgaatta aaaaaatata
     9601 aaaccggtga gggaaaacct ttggtgttga acaatggaga tagctctcaa ttcagcctga
     9661 gcttcttttc agaaaacaaa atgatttcta ccggagattt gtattttgat ttacctgcaa
     9721 aaggaatcac cgtgacagga aaagactcgc agtctgtttc tataaagtta aatgccggtg
     9781 aaggccgtta cattgaatat gtttattcat tgaaaggtga tgattatcgt gtaggcttta
     9841 aaattaactt cgtgaatatg gggtcattgg tagcttccaa tgcaggttat atggaattaa
     9901 attggaatca gaaattactg caacaggaaa aaagccataa aaatgaaagt gctgtttcaa
     9961 caatttacta tcgctatact gatgaagaag ttgaccattt gagcgaaact aaggatgatg
    10021 gaacagaatt aaaaaccaag gttcaatggg taggatttaa gcaacaatat ttttcaagtg
    10081 tattaattgc tgaccaggct tttgaaagcc caacaagaat taatagtaca gtaattaatg
    10141 atgaaggtgc tgtgcgaatg atgcacgcca attttacaat cccattcagt catcagaata
    10201 atgaaagcac aggaatgagt ttttattatg gtcctaacca ctatcaaacg ctaaataaat
    10261 atcacatcgg aatggaaaaa cttgttccat taggatgggg aatttttggt tgggtaaatc
    10321 gctttgctgt tattccagta ttcaacttcc tgaatagttt taatatcaat tatggtatta
    10381 tcattttact tctgaccata ctcatcaaag ttgtgttgtt gccgttaact tacaagtcat
    10441 acatcagtac tgcaaaaatg aaagtgttgc aacctgaaat gtcggagttg caggcaaagt
    10501 ttaaagcaga cccaatgaag ttgcaacaag aaacaatggc actttaccgc aaagccggtg
    10561 taagtccgtt agggggttgc ttacctatgg tgttgcaaat gcctattctc attgccatgt
    10621 tccgcttctt tcctgcatct attgagttac gacaggaagg ttttttatgg gcgaaagatt
    10681 taagcaccta tgattctatt ctcgatttac ctttcaacat tccgttttat ggagaccatg
    10741 tgagtttatt tacaattctc atgacagtgt caacactcat ctatacacgc atgaatatgc
    10801 aaatgactgc tgccacaaac ccttctatga aatatgtgat gtacctgatg ccgataatgt
    10861 ttcttggaat tttcaacaat tactcttccg gattgagcta ttattatttt cttgcaaata
    10921 tgattacctt cggacagcaa tatctcttta aatcttttgt tgatgaaaaa gctattcatg
    10981 caaaaattca ggaaaacaaa aagaagcctg ttaaaaaatc gaagtttcag gaacgattag
    11041 aaaaaatggc aaaagagcgt ggtgtaagaa agtaataagt ttgaatgttt taaagttgaa
    11101 aggtttgaaa gttatataag actgaaatga atatctaagt aaatcaatga tttgatgtta
    11161 tattagaatg ttattggata aatttaaatt tgaaacattg taatattact ttttactttg
    11221 taagaatcaa tctaaacaca ttttaaatta aatcaataat tcaataacac aataattcat
    11281 ccaattaatt ctctaaataa tatgaataaa gttgtaaaaa atgttgacga ggctttaaaa
    11341 gattttcgtg atggcatgac acttatggtt ggtggctttg ggctttgtgg tattcccgaa
    11401 aattgtattg cagcattagt tcgaaaaggc attaaaggat taacctgtat atcaaacaat
    11461 gcaggtgttg atgattttgg cttgggatta ctcttgcagc aaaagcagat caagaaaatg
    11521 attgcaagct atgttggcga gaatgcagaa tttgaaagac tgatgctgac gggagaaatg
    11581 gaggttgaat taattccaca gggaactttg gctacaagat gtatggctgc cggctatggc
    11641 atgcctgcaa tctttacacc tgccggtgta ggcacagaag tggcacaagg aaaagaagta
    11701 agacgtttca gttacaatgg cgttgaaaaa gattatctga tggaatatgc atttgattct
    11761 gatgctgcat tggtaaaggc ttggagaggc gacacagaag gaaatcttgt ttaccgttca
    11821 acagcgcgaa attttaatcc aatgatggct atggcaggaa aaatcaccat tgcagaagtt
    11881 gaagaattag tccctgccgg aacacttgac cccgatcaaa tccacacacc cggaatttac
    11941 gttcaccgta ttttccaagg cgagaaatat gagaaaagaa tagaacagag aacggtgagg
    12001 aagaagtgat tagataattt gaacattaga taattagata attacttgaa atgtcaagaa
    12061 aatttgattc tccttttgaa gataataaaa tcctgaaact cacttttgag ttttcgttaa
    12121 aaatcattca tctttcagaa atattaaatt caaatcacca atatatcatt gcaaatcaaa
    12181 tcttaaaatc agcaacttca ataggagcca atgcttatga agctcaaaat gcggaaagtg
    12241 aaccagactt tattcacaaa tttaaaatag ctgctaaaga aactgaagaa acttactatt
    12301 ggcttctttt gtgtaagcat gccgaatgtt atccagacac tactgattta ttaatcgaat
    12361 tagaagtaat taaaaaaatc atcaataaaa tcatttcaac ctcaaagaat aaattgtaaa
    12421 atagcaccgc acattcaatt atcaaattat ctcatcatct aattatctaa ttaattatgc
    12481 tcgataaaaa cgcaatcgca aaaagaatag cccgagaagt taaagacggc atgtatgtaa
    12541 accttggcat aggcatccct actttagtcg ctaattatat tcccaaggga ataaatgtaa
    12601 cactacaatc agaaaacggc attcttggca tggggccatt cccttttgaa ggcgaagaag
    12661 atgctgactt aattaatgca ggtaaacaaa ccgttacgct acttcccggt tcagtgattt
    12721 ttgattcggc aatgagcttt ggaatgattc gtgctcagaa agttgattta accattctcg
    12781 gtgcaatgga agtgagcgaa gaaggagata ttgccaactg gaaaattccg ggtaagatgg
    12841 ttaaaggaat gggtggcgct atggacttag tagcatcagc aaaaaatatt attgtagcta
    12901 tgcagcatgt taacaaagca ggcgaatcaa aacttttacc taaatgtact ttgcctctga
    12961 caggaattaa atgcataaaa agagttgtta ctgaacttgc agttcttgat gttactccac
    13021 aaggatttaa attgcttgaa cgcgcccccg gagtttctgt tgaagatatt aaaaaagcta
    13081 ctgccggaaa acttattgtt gaaggtgata tccccgaaat ggtgatagat taactttctg
    13141 cattgatgag ataccttgac cattttgaaa aaattgctga gaaaagaatt cgtgaccggc
    13201 ataaacatcg ttattattgg aacgatataa ctttttactg caattatttt tctcatgaaa
    13261 cattttctgt actcgaagtt ggctgtggca ccggtgagtt gttaaacaac atccgtgctg
    13321 cccaaaaaac cggcattgat tatagcccta caatgattgc cgaagcaaaa aaacaatttc
    13381 cacagattga ttttcaggtg atggctgcag aaaatatttc actcgaagag aaatatgatt
    13441 taattattct ttcaaacctg acaggctacc ttgaagacgt acaagctgtt cttgaacaat
    13501 tgaaaaaagt ttgtcatgag cgtactaaaa tcattgtatc ctatcacaac catctatggg
    13561 agccattttt acgcttagca gaaaaccttg gcttgaaaac caagacccca caattgaatt
    13621 ggctcaccag catggatgtc cgcaatcttc tgtttcttgc aggctttgat tgttatcgag
    13681 agtcgaggcg catgttgttg ccaataaaca ttccgctctt atctccactg ataaataagt
    13741 ttatagcaaa gctgccttta ttcaggttga tgtgtatcaa cacctactct gttgctaagc
    13801 ccttggcaaa tgcaacagaa gatgtcaacg aaaaatattc agtaagtatt gttatccctg
    13861 ctcgcaatga aagcggcaat attgaaaatg gattgctacg tattcccaaa tttggcaagt
    13921 ggcaggaagt tatttttatt gaaggcaaca gcaccgatga cacctgggac agcattaacg
    13981 aagtttccag taaatatgct gcttcacata aaattaaagt tggtaaacaa gaaggcaaag
    14041 gaaaaaacga tgctgtacgc aaaggcttct caatggcaga aggcgacatc ctgatgattt
    14101 tagatgccga ccttaccatg cctccggaag atttgcccaa attttatgaa gccattgcca
    14161 gcaataaagg agattttatt aatggctgcc gtttaattta tccaatggaa aaagaagcca
    14221 tgcggttttt aaatatgctc ggcaacaaat tcttcagtca ggcttttacg tggttattag
    14281 agcaaccttt taaagataca ctttgcggca ccaaagttat tttcagagat gattacgacc
    14341 ggttggttac caacagaaag ttctttggcg actttgatcc ttttggtgat tttgatttga
    14401 tttttggtgc ttacaaactc aatcttaaaa tacaggaatt acctatccgc tatcgcgaac
    14461 gcacctacgg cagcaccaac atatcgcgtt ttaaacatgg catcattttg ttgcgcatgt
    14521 gtttttttgc tgcaaggaaa atcaagtttg tgtagaaatt tccttcttca ctgcactctc
    14581 caactccgga aaaaactcct tgaagtgttt ttcaaacagc tcatagttat catacaaaaa
    14641 ttctgctgcc tgattcatgt tattatcaaa ctttgcacgt ctgcccatac cactcaacgc
    14701 tctatgcatt ccgtttaatg tagcgtagtt tttcaaccag ttgtcgttct ccatataaac
    14761 cagcatttct ttggtacgtg caggtaaaaa ctgataattc ttatgcataa gttgataaac
    14821 atatcttgca tattcatcaa ttggttgatt ggaatattta tcccattctg ctgccagaaa
    14881 atgatcgtaa aaaatatcgc tgattaccgg tgtatattta tgaaagtaag gacgcaacag
    14941 taccttgctt tgttcaacaa caggatgtgt atctgtaaaa tgatcaataa aacgatgtac
    15001 ttttacaccc gacattattc cttcaggcaa acttttccag tcatttccct ttaaatgatc
    15061 agcaataaaa ttacctacca taagtggctc attattgtct gaaagataaa gatgtgccaa
    15121 aaaattcatt gggctaattt agcaaatagc ttctttgaaa atgtaaacca taaggaatga
    15181 ttacctttgc aattgattaa attatgcagg tactccaaaa aacatcacaa tatttatcgc
    15241 ttgtaaaatt cagccatact gtgtttgcac ttccttttgc tgcaattgga ttttcgttgg
    15301 ctatttcaca aaacgggctg cctgtttttt ggccgttggc aataaaagtt ttagtctgca
    15361 tgataactgc acgtaatgca gctatggcat ttaaccgatg ggccgataga agtattgatg
    15421 cattgaatcc acgaacagca ctgcgcgaaa ttccggccgg catcattaaa gcaaaccgtg
    15481 cattgtggtt tgtaattgtt aatgctttac tttttacagt gactgcatac ttaattaata
    15541 aactttgctt ttttctgtcg cccgttgcat tatttgttgt gcttggctac agttttacca
    15601 aacgatttac agcactttgt cacttggttt taggcgcagg acttgcattg gcacccgttg
    15661 gcgcttatct ggcagttaca ggaaagtttg atattactcc aattttaatt ggtgttgctg
    15721 ttctttgctg ggttagcggg ttcgatatta tttatgcttt gcaggatgag caatttgatc
    15781 gtgaaaataa attgcattcc attccatcac tcatgggaaa aaaacaggca ttgcaggttt
    15841 caaggatttt gcatttttta tcggcaggtt ttttggctct ttttctttat caggctcatg
    15901 taggtttaat tgcatggtca gggcttgcaa tattttgtgc actgcttatt tatcagcaca
    15961 gtttggtttc tgaaaataat cttagcaaag taaacattgc ctttgctact accaatggaa
    16021 ttgccagcat tgtttttgcc ggtatggtta tttcagatat ttttttactt cattaagaat
    16081 tacttcctaa cccttaaaaa cggttacagg cgcagagaac caccaactgc gcctgtgaaa
    16141 aacaaccaac catgaaaagt attgatacta cattgttcgc gtaacgattc ttgtaaagat
    16201 ttttttgtct gtggttactt ttactaaata atttccgggt ttcaatttag atgcattgac
    16261 agcaatttca ttaacctgct gttgttttaa atcaggatta agctgctgag caatttttcc
    16321 atccattgta taaacaataa tgctttgtat atcgttaacc ggtgtactga atttcattgt
    16381 aaaattgtta ctgaatggat tgggataaat ttccatcatt gcattatctt cattcgcaaa
    16441 agcagttctt aacattgcat cagtatagtt tattcctaca agtacatggt gttccatagc
    16501 acaaccatta ccatctttca ttcgcactgt gtatgttccc tgcgataagt ttgctaaatc
    16561 agcaacggtt tgaccattgc tccattgata ttttaacgga ggcattcctc ctatcacctg
    16621 caatttaatg ctgccatcat tttttcctgt ataactttca tcaagtactg ttgcaaatat
    16681 tcctattggc tcaggctgct taacagtaaa agtatttctg taaactttat tattggcatc
    16741 ggtaatgagc acactataat cactggcagt aagattactt attgttgctc cattcatacc
    16801 atttgaccat tgaaatttat aaggtgcaac tcctcctttt acattcagtt ttattgaacc
    16861 atttttccct ccattacaca attcatgttt cacaaccgat tcaacctgca taggcgattt
    16921 ttcattcact gtaaaatatg ctgctgcact acaacggtgc gcgtcatcaa ccctaacagc
    16981 ataaacgcct tcttcaacat tattcaacga ctgactgctt tccatattcg accacaggta
    17041 ggtgtaattt cctgttccac ctgaaacctg taacattatg ctaccattgc ttttattcaa
    17101 atccgcctct gtaacttttg cttcaacttt tatcgtttcc ggatttttta attgaacttc
    17161 tgcttttgca gtgcaaccat tattgtcttt tacgatcaca gaataatttc ctgcttttaa
    17221 tccactctga tcttctgttt tagcaccatt cgaccattca taggtatatg gagaccatcc
    17281 acctttaact tcaacatcta ttaagccact ggcttcgtca ttacataaag gagaatactt
    17341 tgctatgttt aactcaagaa ctttgtttct gtcaatagta atgttcgact gctttataca
    17401 gccattctta tctgtaactt ttatttcata atcgcctgcc gcaactgcct ccaagttttg
    17461 ctgacgggtt tgattcgacc attcatactg atatggttgt gttccgcctg ttaccaataa
    17521 gtttgcttta ccattgcggg cataacactg ttcggaagta acttgcgaat aaattgtcat
    17581 agcataaggt tcagtaagtg taatagactg cgaaaccgta ctgcctttag aatctgtaat
    17641 tgaaacagaa tactcgcctg caggcttatc taacatggca tagcctgacg taccatcatt
    17701 ccatgtaatt tgatatggcg ccttaccgcc tgaaatatca agactgatag agccattgct
    17761 gccgccaaaa caagagacat tctggccttt taatgttgct ttaagtgttt ttccttttgc
    17821 ataagcagga aatggattgc caatataact gaacataatc agtatgatgg ctgcttttgt
    17881 tgtgttttga attgttctaa tcattggtgg ttcaattaag atgcttgcaa gctatcacac
    17941 caccaatgct gcaaggcact gttttatgtt aacatgttaa tttttggggt gaaaattcaa
    18001 ataaaaatgg caaacggttt ttggataatt actccctgaa aggatttcgg gcaatccact
    18061 cctctggtgt aaatcgcctg acaacaggct tcaccatttt attcaagaca gtattactta
    18121 gattaaaata agcatgtaat ggataggctt tggctccggt agtagatttt atctccatag
    18181 cagtccgccc ataataaaga ttagaagagc gttgactgat ggcttcttca ataaaacgat
    18241 ataatatatt ctgataaaga gaaagtgttt tattcaaact gtaatcaata cctataaaat
    18301 gtgcctcatg atgaccggca tgatgaaacc cacaataaaa tgctatcact ttactttctt
    18361 taaaaaaaac ctttacgtaa aatttttcag gcattatggt taagagtttt tcaaaatata
    18421 aaatattgac tctcgccatt tgcacggttg ccttttgctg tatatttaaa tacagcctta
    18481 ttaattcatc tccatatttt agtacttctg ctgcattaag ggcaacaacg tcaacatctt
    18541 caatctttct tattacacta ttggctttaa cacgatattt tgttgtgagc gatgccaggt
    18601 attcttcgaa cgaatgccac tgttctctga tgtataatat catctctggg tccattggca
    18661 attcaaaaaa atcatcttcc agcaatggtt gatcaacctt ggtgttgtta taaaaatcct
    18721 ttaccatcca tccacacacc gatgcattac taattgtatc tgtaattttc tgtctcactt
    18781 ccggtaaaag ttgaattact gcaggcaagg catctctatt tataacattg ataccatatt
    18841 caccgcttac aaaaaaactt ccgcaacaaa ttaaaaagtt tacttttccg gtttggcatt
    18901 taaataacag cctgttgatt gatttattca gattctgcaa caaccaacct ttgtctttta
    18961 aattcaatac accatccaaa tctttattgg caacatcaag caattgaaaa cacaaagtcg
    19021 caacaggcat cttactatca taaacaataa catatctaaa ctgcatgccg gcaggcttgc
    19081 tttgttctaa tgccgacaaa taagaaaatg aagtaaaaat gacattttca ccatttatgg
    19141 tctgccagtc atttgccggc atttccctca cactattaaa aacttctatt cttaacatat
    19201 tgctcgtcaa tattctttta gtcccgatat ttgctcacag gctcatttaa aacattaaaa
    19261 aaaacaaagc acgtaaaaat tatttcacgt tatttgtaac agttaaaagt aaagttagtt
    19321 atgaatatta agtacaattc catcaacagc aagttatttg ttgacaacag gaaaaaattt
    19381 acagcagaat taaaacctaa atcaattgct atatttcaat ctaatgatga aatgccgcgt
    19441 agtggcgatc agaatttcac ttttcgtcag aatgcagatt tgttttacct caccggtatt
    19501 gatcaggaag aaagtatttt ggtaatattt cctgactcac cattgccgca atacaaagag
    19561 atattatttg tcagaaaaac caatgagcgc attgccatct gggaaggaca taaatacact
    19621 aaggaagaag cacaggcaac atcaggtata aagcatatct attggacaga agagtttaac
    19681 gctgtcgttc ctatgctgat gcaccatgca gagaatgttt atgtcaacat taatgaaaac
    19741 gatcgtcatg ctacagaagt gccttatcgc gatatgcgtt ttgcacgtga atggaaaaac
    19801 cgttttcctg cacataattt tgaacggctt ggtcagataa tggcaaaact gcgttcgcga
    19861 aagcatgata tcgaagttca gttaataagc gaagctatta aacatacgga atcggcactc
    19921 agacgtgttt taaaatttat gaagcccggt gtgatggagt ttgaagttga agcagaaatt
    19981 attcatgagt ttgttcgcaa tcgttgcaat ggacatgcct acagtcctat aattgcctcc
    20041 ggtgcttctg catgtgtttt gcattataat gaaaacaaca agccttgtaa agatggcgat
    20101 ttagtgctga tggattttgg ggctgagtat gcaaactatg ctgcagattt aacaagaact
    20161 attccggtga acggaaaatt tacgccacga caaaaagaag tatatgaggc tgttttgaga
    20221 gtgatgaaag ctgcaaccaa acttcttgtc ccaggcaact ttatagaaaa gtatcatgcc
    20281 gaagtaggta aaatgatgga agaagaattg gtgaacctta aactgataac taaggacgaa
    20341 attaaaaatc aaaatccgga gtggcctgct tataaaaaat attttatgca tggcacatcg
    20401 cacttcctcg gacttgatgt gcatgatgta ggcaataaat atcaggctat gcaggcagga
    20461 atggtgttta cttgtgagcc gggcatttat atccctgaag aaagcattgg aatacgtatt
    20521 gaaaataaca ttctcatcac cgataaagca ccagttgacc tgatggccac aatgcctgtt
    20581 gaagtaagtg acattgaatc attgatgcgt aaataatcac aaaaaataat tagaaaacat
    20641 actctacaaa tatgcaaaaa gacaaagctg tttttaaact gattaaagaa gaacttgcac
    20701 gccaacgtca tggtttggaa ttgatagcat cggaaaattt tgtcagccaa caagtgatgg
    20761 acgccatggg ctcatgtctg acaaacaaat atgcagaagg acttcccggc aaacgctatt
    20821 atggcggctg tgaagttgtt gatgaaattg aacaaattgc aattgacaga gctaagactt
    20881 tatttaatgc aacatgggca aatgtgcagc ctcattccgg agcacaagct aatgcagccg
    20941 taatgcttgc atgcctgcaa cccggtgata caattcttgg ctttaacctt tctcatggcg
    21001 gtcatttaac gcatggttca cctgtaaatt tttcgggcaa actttatcgt ccaacatttt
    21061 atggtgttga agaaaataca ggcactattg attacaacaa agttgaagaa actgctttaa
    21121 aagagaaacc aaaattaatt atctgcggtg catctgccta ctctcgcgat tgggattatg
    21181 ttgcactacg ttccattgca gataaagtga atgcattgtt gcttgcagat atttcacatc
    21241 catcaggact aattgcacgg ggattactta atgatccatt gccacactgc cactttattt
    21301 ctactacaac acataaaact ttacgtggcc cacgtggtgg tttaatcatg atgggaaaag
    21361 attttgaaaa cccatgggga ttgaaaactc caaaaggcga aacacgcatg atgactgcct
    21421 taatggacag tgcagttttt ccgggcacgc aaggtggacc attggaacac gttatcgctg
    21481 ctaaagctgt agcatttggt gaagcattaa gcgatgagta ttttgaatat tgcctacagg
    21541 taattcgaaa tgctaaaact atggctaatg cctttgtgaa aaagggatac aaggtaattt
    21601 ccggtggaac agacaatcat ttaatgttga ttgatctgcg ttctaaaaac attacaggaa
    21661 aacaagcaga aaatgctctt gttcgtgcag atatcaccgt taacaaaaac atggtgccat
    21721 tcgacacaca atctcctttt gtaacttcag gtatgcgtgt aggaacaccg gcaatgacat
    21781 cacgaggaat gaaagaaaca gaaatggctc gaattgtaga attgattgat atggtaatta
    21841 tgaatcatac gaatggcgct gtaattgaag aagtaaagac tactgtcaac gacatgatgg
    21901 aacattttcc tctttattaa tttattttgt ttgataaaaa tattttttgg cgattagcgt
    21961 ttgagagtgc ataaaccaaa ttccatgaga ataacaactc tgatagcagc actgttgctt
    22021 cctgtttgtt tgaaagctca gacaggcgga agtgcaatct ttcgttttct tgacctgcca
    22081 tcatctgcac gtcatgcagc actcggtgga aatttcattt ccattcgcga caacgactta
    22141 aatacttcct tttacaatcc tgccatatta aatgttgata tggataaaca gattaccttc
    22201 aactacaatc catatttctc cggaataaaa tatggttaca ctgcctatgc acatcacatt
    22261 gaaaatatag gaacactttc tgccggcata caatatgtaa gctatgggaa gttcgaccgc
    22321 acagacgaaa ctgcaactga cttaggaact ttttcaggtg gcgattatgc attaaacata
    22381 tctatgtcaa gacctgttgg aattgatagt gtgttgtttg ttggtgcttc attgaaattt
    22441 atttacagtc acattgacac ctactctgca tttggaattg ctgccgacct tggagtcaac
    22501 tattacagtc ccgataaact cacaagtgct gcacttgtta tcagaaatgc cggtcgtcaa
    22561 ttaaaatcat atactgagaa aaacaatgag ccactgccga ttgaaattga agcaggcatt
    22621 acacagaaat tagccaaagc tccttttcgt tttactttaa cctatcgtca tattgaaaaa
    22681 tgggatcttt cctacattga caacaacagt caggaaacag accctctgac aggtgaagta
    22741 aaagagccgg aaaaagcatc gtttggcaat aaactattgc gacatttaat catcagcaat
    22801 gagattctta tcagtaaaaa ttttcatttg cgcatggcgt acaatttcca gagacgaaat
    22861 gaaatgaaag tagaaagcag acctgccatg gttggtattt cattcggact tggtttaaaa
    22921 atatctaaat ttcacctcag ctatggcaga agcctttacc atcttgctgg tggaaccaac
    22981 agcctttcta tttcaacata tctaaacgat tttaagcgca aaccaaaaac aactgacagc
    23041 taacctgaaa taaagttaat attcactgtc gttatattgc ttttcattat ttttgaaaag
    23101 ttttccggat aagttttatg ataattgcta ttgatggtta ttcttcctgt gggaaaagta
    23161 cggttgcaaa agccatagca aagcggctta aattgaatta tattgattcg ggtgcaatgt
    23221 accgcgcagt cacttattat ttcctgcaaa acaatattcc attcagcaag actgctgatg
    23281 taattgctga ttattcttca attcttgaaa acattcaaat tgaatttatc agaaatgaac
    23341 aaaccggaca atcagaaatc tatctcaatg gcaaaaatat tgaagcagaa atcagacaga
    23401 tggatgtttc agacaatgtt agttatgtga gtgcaataaa atcggtacga aaaaaattag
    23461 ttgccatgca acaacaaatg tcaactcttg gaaatctggt aatggacgga cgtgatattg
    23521 gcactaccgt ttttccgaat gccgacttga aaatttttat gactgcatca cccgaagtac
    23581 gtgtttacag acgatatcat gagctcattg aaaaaggaat aaaagtaaca cctgatgaaa
    23641 taagaaaaaa tattgagttg cgcgactatg aagacactca cagagaagaa agcccattgc
    23701 gaaagcctga tgatgcaatc attcttgaca acacctatat gagtcgtgat gaacaagtac
    23761 agtttgttct ggatgctgtt gctaagttga aaaaggcaat ttaattcaac tcaaacaatc
    23821 tcttacattc tttccggggc cttaatacca agcaggttca atgatttttt aattgtcatt
    23881 gcacaggcat cagccaaaca cagtctgaac tggcttgttt ccaattcacc ctctgaaata
    23941 attgaattat cgtgatagaa ttgattaaag ttttttgcta actcataaac atagtttgca
    24001 acaatagccg gagaaaattt ttctgctgca gtttcaactg tttctttaaa acgaagaata
    24061 ttgattgcaa gttcacgctc cttctcattc atgctaacaa gtgattctat tgcggtattt
    24121 ttatagttgc tcagttgttc catctgtgaa gcccgcttca aaacagaaca aattcttgca
    24181 tgcacatact gaatataagg acccgtgttt ccatgtatct ctacactttc agcaggatta
    24241 aataacattc ttttttcagg atcaactttt aaaataaaat atttaagtgc cccaaagcca
    24301 agcgtttcat agagctgctt taattcatca ctgctaaact cttcaacctt gcccaactta
    24361 cttgtttctt cagcggcagc agcagttact tcgtccatca aatcgtctgc atcaacaaca
    24421 gttccctcac gtgatttcat tttaccactt ggcaaatcaa ccattgcata acttaaatga
    24481 taaagctttt cagcccattc ataccctaat tttttaagta ttaaaaacaa tactttaaaa
    24541 tggtagtcct gttcattgcc tacaacataa attttagaat cgaaatcaaa ctcatcatga
    24601 cgcaacactg cagtgcctaa atcctgcgta atataaaccg aagttccatc agagcgctgc
    24661 actatttttt cgtctaatcc atcattcgtt aaatcaaccc aaacgctgtt gtcagccttt
    24721 ttataaaaca caccttttga cagtccttct tcaactattt tctttcccag cagataggtt
    24781 tccgattcat aatatgtctt gtcaaaatct acacctaatc tgttataggt aacatcaaaa
    24841 ccttcataca cccaaccatt catcttcttc cataatgcag tcacttcttc attgccttgc
    24901 tcccactcta tcagcatctg ccttacttcc tgcattaagg gtgcgttctt ttctgcttta
    24961 tctttttcaa tacctgcaga aatcatttct gaaacctgct gtttatattg cttgtcaaac
    25021 tccacataat attttcctac aagatgatca cctttcattc ctgatgattg aggtgtttca
    25081 tcattaccaa attttttcca tgcaagcatt gatttgcata tatgcacacc tctgtcgttg
    25141 atgatgctaa ctcgtttaac atttcgtccg gttgctttca gtagcaatga aactgaataa
    25201 cctaaaagga tatttcgcaa atgtccaagg tgtatgggct tgtttgtgtt tggcgatgca
    25261 tactcaacaa taacaggctt agatttatct gctttagtaa aaccatattc ggaatctgca
    25321 aatgcaactg caattctttt aagccactgc tcatcactta ccgtaatatt caaaaatcct
    25381 tttatgacat tgtaattgct aataaaagat gcatgtttga ttaaatactc tccaattaat
    25441 cttcctgttt cttcaggcga ttttttcgac tgacggacaa aaggaaaaac aactatagta
    25501 taatctccat caaactcctt acgagtttgc gtgtaagtca acagactttc atccggctca
    25561 aaattaaaaa gcacttttaa tgcttcattt gttatgctcg ctaattcctg ttcaaaaatc
    25621 atttcaaaat agattaatta tagatggcat tttttgccgc aagtaaggta ttctgcaata
    25681 atccgataat ggtcatcgga ccaacacccc ccggaaccgg tgtgataaaa ctacacttag
    25741 gggctacttc atcatactta atatctcctt taagtctgta cccgcttttt gttgctttgt
    25801 ctgctacacg tgtaatacct acatcaatca ctactgcacc ttctttcacc atatctgctg
    25861 ttatcaattc aggcgaacca attgctgcaa ttaaaatatc agcacgtttt gtatatgctg
    25921 caagatctac agtcttgcta tgacaaattg ttactgtaca atttccgggc ttactgtttc
    25981 gactcaacaa aatactcatt ggcattccaa caatattact tcgcccaact accacgcaat
    26041 gcttcccctg tgtttcaatt ttatattttt caatcaactg cataatgcct ttaggtgttg
    26101 ctgcaatgta acatggtttg ttctgttgca gcctccctgc attcatcgga tgaaatccat
    26161 caacatcttt tgcaggatga atagcttcat ttattttctg tggtgaaata tgatttggaa
    26221 acggtgattg cacaatgaca ccatcaatgc ttgaattaaa attgagttca tttatacagt
    26281 tcaatatttc gttttcactc accgtatttt caaaacgaaa aagcgatgca ttaaatccaa
    26341 catcatgaca agtctttaat ttattagcaa cataagtttc gcttgctcca ttattaccaa
    26401 tgagtaatat agctaacccc ggaggacgct tgccggttgc aacaagtgct tttacttcct
    26461 gctttagaat agatttatat tcttctgctg tttgctttcc atcaattatt gtcatgccct
    26521 aaaaatattg tcctaatgcg ataacttatc ttctcattcc gggcatattg cgcatcatgt
    26581 ttttcatatt acctgtcgtc atcatcttca tcatcttgcg catatcctca aactgtttaa
    26641 tcaatttatt tacttcagga atacctgtgc cgctaccggc tgcaattctt ttctttctgt
    26701 taccattaat cagatcagga ttggcacgtt ctgccggtgt cattgactga atgattgctt
    26761 caatgctttt aaaagcattg ttgtcaatgt ccatgtcttt aactgcctta ccaactccgg
    26821 gtatcattga aacaaggtct ttaatgtttc ccattttttt aatctgctgc aattgcgaaa
    26881 ggaaatcatt aaaattaaat tgattcttgg caaacttctt ttgcagctcg gctgcttgct
    26941 tctcatcaaa ttgttcctgt gctttttcaa ccaatgtcac aatgtcgccc atacctaaaa
    27001 tacggtcggc catacgctca ggatggaaaa catccaacgc atccatcttc tcaccggtac
    27061 cgacaaactt tatgggctta tttactactg aacgaataga caatgcagca ccacctcttg
    27121 tgtcaccatc aagtttagtc aaaaccactc cgtcaaaatt caatcggtca ttaaatgctt
    27181 ttgctgtatt cactgcatcc tgaccggtca tactgtcaac cacaaacaag atttcatcag
    27241 gttcgattgc atttttcaat gctgcaatct cattcatcat ctgctcgtca atagctaaac
    27301 gacctgcggt atcaacaaca actacattgt taccattctg cttggcttgc tttattgcat
    27361 gacgcgcaat ttctacagga tttttatttt ctacttcaga ataaacttct acacctatct
    27421 gttcacccaa aactttaagc tgatctatgg ctgccggtct gtaaacatcg catgcaacca
    27481 acaaaggtgt ctttcctttt ttagtcttta aaaaattagc aagttttccg gataatgttg
    27541 ttttacccga accttgcaac cctgacatta gaatgactga tggatttcct ttaagttgaa
    27601 tatctgtcat ctgaccaccc atcaatgtag ccagttcatc atgtgcaatc ttaaccaaca
    27661 actgaccggg agatactgcc gttagtacat tttgacctaa tgccttctct ttaatcgtat
    27721 cggtaaattg tttggctact ttataattta cgtcagcatc taataatgcc cttctaacct
    27781 cttttagagt ttcggcaacg ttaatttcgg ttatctttcc ctgacctttt aggttcttaa
    27841 acgccttttc taacttatca cttaaattct cgaacatgat taataaaata gtgtgcgaaa
    27901 ataagaaagt ttaaccgttt tttatgaata taaaatcagt ctgaaaaatt gatgtgaatt
    27961 tttctcagga caacacaatt tctaactgtt aaagtttgtt aatggatata gatttccatt
    28021 aattaatata gctttgaagc tctctcaatc aaacaattct taatttcgca acgcagttac
    28081 cggaataaca tgaaaccttt taaatctatt tttttattta tattattttt cagtgaggct
    28141 aacgctcaga aaatctggtc gcttaaagac tgtattgact atgcattgaa caataatatt
    28201 agtgtgcagc aatcaatcat tgcttcaaaa attgcagcag ctacttatca gcaaagtgtc
    28261 ggcagccttt ttcccagtat aaatgccagt tcttctttaa attacaacta cggaagaagc
    28321 cttgacccta caagttatca atatgttgat caaacttttc agtcaggtgg tgcatcttta
    28381 aatggaaatc ttccaatatt tcaaggactg gaattgcaac ataatttacg taaaagttcc
    28441 attgattttg atgtaagtaa actggatgta cgaaaagtgc agaatgatat tgcattatcg
    28501 gttgcagcag cctatcttca gatactttac agtaatgagc aattaaaagc aactaacgac
    28561 agaatagaag cagttactaa agagcgtaac agagcaaaaa ttttagttga caatgatttg
    28621 ttagctgaag gctcattgct tgatgccgat gcacttcttg caactgaaga attcaataaa
    28681 gcttctgcgg aaaatctgct cacctcatca aaactcacgc taattcaatt aatgcaactt
    28741 gaaggtgcga atgacattgc aatagaaact cctgcactgg aaatcccatc tcaggaatcg
    28801 ctttcattat catcagaatc tgtttacaat gaagctttaa aaaatctgcc ggaggtcaaa
    28861 gcttctgaac ttagattaaa aagtgcaaaa gaatctttag gtattgcacg tgctgctcgt
    28921 tatccaaggt tatctgcatt tggctcttta tctaccaatt attccaatca ggcaacatct
    28981 ctcgatggca atcctgtttt ttcaggatat gtaccgtcag gcgcggttac atcatcagga
    29041 gagcaagtgc tgcaacctgt tttcaattat aaatatattg atactcctgt taaagatcag
    29101 ctgaacaata atttcggaaa agctatcgga ttcaacttgt ccatcccctt atttaatgga
    29161 tgggcaacaa acagcagcat taaaagatca aaattgagtt ttgaaaatgc acgcctcaat
    29221 gccttgcaaa ctaaaaacac tgtgtttaaa aatgttcagc aggctcatgc cgatgcattg
    29281 gctgcactca acaaatatgc tgccgcaaac aaaagtgtta cagcacagga aaaagcattt
    29341 ttatatacag aaaaaaagta caatgctggt ctattaaatt ctattgacta cgtaaatgcc
    29401 agaaataatt acaccaaatc acaatcagat ttgattcaat caaaattcga tttgatttta
    29461 aaaataaaag tacttgacta ttatctggga aaaccattgt cattttaagt aaattgtatg
    29521 agtaagaaaa ataatacatt aagaaatatt atcatcattg ccggagtcgt tattgtggct
    29581 ttggtaattg ccaaaaaagc gggatggctt ggcgaagaaa atgtcaccaa tgtaagcact
    29641 gaaaaggcat cattacaaac catcacagag tctgtatctg caagtggaaa agtacagcct
    29701 gaagtagaag taaaaataag ctctgatgtt tcaggcgaga ttgttgagat gcttgttaaa
    29761 gaaggtgatg tagtaaagaa aggtcagttg ttatgcaaaa tcaatcctga aatatacata
    29821 tcatctttag acagagctgt tgccgggctg aattctacaa aagcaaatta tgaaagcagc
    29881 aagtcacgac tatcacaagc agaaagtcag ttggtaaaag ctgaaattgc ctacacaaga
    29941 aataaaaaac tttttgaaga aaaagtcata agcgcatccg atttcgaagc tataaaatcc
    30001 gcctatgaag ttgcaaaagc agaagtagat gcatccaaac aaggcgtttc aggagcacaa
    30061 tataacatca acagtggaag tgcaactgta cgcgaagcca aagaaaattt aaacaagact
    30121 gaaatttttg caccggtaaa tggcaccatt tctaaaataa gcaagaaaaa aggtgagcgt
    30181 attgccggaa caagctttac tgatggcaca gaaattctaa gaatttctaa cttgcaggaa
    30241 atggaagtaa aagccgatgt aggagaaaac gatatcatta gaatacattt aaatgatact
    30301 acattagttg aaattgacgc ctatagccca cgaaaattca aaggcattgt aacagagatt
    30361 gccaattcac caaatgagtc attgacatca acagagcaag tcacaaactt tgcagttaaa
    30421 atcagaatct tgcgtgaatc ttattctgac ttgattcctt ctgaccatcc ggaatattct
    30481 ccatttagac ctggcatgtc agcaacagtt gaaattcaaa ctaaaaaagt aagtaatgtt
    30541 gtttctgttc caatcaaggc agttacaaca cgtgatacca ccatcagaac atctgacaaa
    30601 gctgttgtca gaaaaaatga cgatactcca cctgatgaca aagtagttgt aagcagtaac
    30661 acaaatgata cacctgttaa agaaatagtc tttgtaaatg acaatggcat tgcaaaaatg
    30721 catgttgtaa aaacaggtat tcaggacaat aattttattg aaattctaga aggaataaaa
    30781 gttggtgacg aagtaattac cgacccctat caggctgtat caaaaaaatt agttgatggt
    30841 gataaaatta aagttgttcc acaaaatcag ctttttaaag aagaacaaaa ataattttag
    30901 tggctcttct actccattta gaaacttcta ctgacacttg ttctgtttgt atttccgaca
    30961 atgaaacaat catagattgt gaagaaacgc ataacagcaa atcgcatgca tctttacttg
    31021 ctgttttcat tgatgaattg ttaaaaagaa accgactgac ttataaaaac attgatgctg
    31081 ttgttgtcag ttgcggcccc gggtcttata ccggtttgcg cattggcgta gccacagcca
    31141 aaggactttg ttttgcatta aaaataccac ttatttctgt tgctacttta cagtcaatgg
    31201 caaacattgc aaacaaacaa tgcaaaggtc agctattatg cccaatgatt gacgcacgaa
    31261 gaatggaagt ttttacagca ctattaaacc cacaactcga ttttatcgaa aaagaaaatg
    31321 cagttattat tgatgaacaa tttcatcatg ataaattagt aaaaaacaag atttgctttt
    31381 ttggtaacgg tgcagcaaaa cttcccgatt caataaaaat caattccaat tgcgtcattt
    31441 tcgatgaatt taaaatgtct gcaatgggct tagtgacact tgcttttaaa aaatttactc
    31501 agcagacttt tgaaaaccta gcctattttg aacctttcta cctgaaagat tttgtcggga
    31561 aataggctgt tttacttgta aatgatctta cgacttcttt catgttaata ttttttttac
    31621 attttttcat aacaattatg tataaataaa tgaaaatgaa taccatttca accgattttg
    31681 cggcataatt aaatattact atattatatt aattgtcaat tgtttaatat atttttatta
    31741 aagttttaat tcatgaaaat taattaattt attaaaaatt cttatcggag cgcagttccc
    31801 atattaccaa cttcttgatg tagatatgat tcgtgaaaaa aaagtggtaa gaaaattgct
    31861 tttcctctta accttttagt aaatttaccg attaaactta cagaggcatc tcattagatg
    31921 tcatccaaaa ctattaaatc aataaaaaca tgaaagattt ccaaaaaatc atttcctttt
    31981 ttatggtcac tttaatcttt gttctcagtt cttaccgttc tgaagcattg atatttcaat
    32041 ctgtggcctc cggaaatttt aacacattga tatggaatca agcaggatta tatccaacaa
    32101 caggtgatac agttgttatc aatacaggaa ctactgtaac tataaatgga ttcaatacag
    32161 atgtattagg atcagtgact attaactctg gtggtaattt gactattgcg aataatgctg
    32221 ttgcacaatt agtatttgcc ggtgacttag ttgtgagtgg aacattgatt aacaacggta
    32281 gaattaatat gactgtaccc ggaaattttg ttttaggaaa tggtgcggtt tatgatcata
    32341 acccaaaaat taatatcgca gttgatgaaa acgtttttga aaatggtgta gaaaatttca
    32401 gccctacaag cactttaata attcgcaaat gggcaagtac tgccttgcca ttggctggcc
    32461 ctactcgtgt aacaagtgat attggaaact tagttacttc tgttaccggt acaacatggg
    32521 aacaacgcgg gcagttttca cctaacagaa ttaaaggcag cctcttagta actgatggag
    32581 ttttaagaat ggatgatggt agcggtgcaa caacagtatt aacattgaat gatgttacac
    32641 tcagaggtaa ttctgcaatt attttccaag aaggtaataa tagaaacctt actttaacaa
    32701 tggggaattt tactgatagt agtgttttag taaaccagcc aactattatc atgaatatgt
    32761 ctttaggaac cctgaatctg actgtcaatg gtaattttat taccagacac gattttattg
    32821 ctgtttttga ctcaacattc ccaatcagaa gtgctagtgt gacagctaat attatgggag
    32881 atttactcat tcagggctca ggtaataaat ttgatttttg caaacaagta aatgcaccac
    32941 ttacattaac cgtaggtggc aatacaaaaa ttgccaataa cccaaattat gttcgttttg
    33001 ttgattccgg aaatggattc ttaaattata ccacaggtga tttaaatatt tctgccggac
    33061 ttaaaaatat ttttttggga ggaaatgatc ttttagatgc ttcaaacaat cctctgtatc
    33121 ctttaccaac tggctatgcc acagtaaatg ttttgaacga cttcaatctt tctggagtta
    33181 ccaatactac catactgctt tctcccggaa atgttgccag aacgaaactt attgttggtc
    33241 atgacttaaa ctcatcatct gcatcagtta attttgttgt tgccaacaac attgggatag
    33301 ttaatattga aatcggctca aatttatttc tgaatggtgg ctcaatgaat gtgcaagcca
    33361 atgcaagttc taatgcaatt gactcaattg ccgttacaag aagttttatt tttaattcta
    33421 caaacccggc aaactttttt agaggtaatt ccggtgcagg caatacattt attagggtac
    33481 taggcgattt taatctgatg aattccggaa cagcagctaa tcagggagtt tgtggaacat
    33541 acctcaacaa tggaaacctt gaattttatg ttgcaggtaa ttttaatcaa agtgctggtg
    33601 cattttatgg tattcaggac ggaacaggtc gtaatacatt taccgttgat ggaaccttaa
    33661 accaatcagg aggattcttt aaaggcacac atgcaaccaa catttttaca gccggaaaaa
    33721 gtatttttac tcttggagga tttagctttt ctggaggcaa ttacattcaa tacaatgctg
    33781 caaacgacag tgttatttgt actgtaaatg gtgatttcaa tgtaaattat actgctgtta
    33841 caaacttagt aagttttatc ccctacctac atccggttta tagtaacact tctccattaa
    33901 aacttaccgt tacaggagat atgtatttcg gaggtgctaa cggcactttc tattccagtg
    33961 taactcaagg taacgaatta atcagcattg gagcaaattt aacatttgac aacgggaata
    34021 acagttttaa taattatcct agtgctgtta ttacaaccac acataatgtg aatataactg
    34081 tcggaggtaa tttaacctgt aacaatggaa taaataattt ttctgccggc agaggtgatt
    34141 tagcaggaac aataaacgga gacctgatta tttcaggagg cacattaagt ttgaaaagca
    34201 actctgctta taatcctatt atcattaatg ttttaggtgg atttaatatg acaaacggca
    34261 attttttcta tacaaacaat gcaactatcc ctcctattac gactacagtt acaattaaca
    34321 gtgatgacga taatacaggt gattttgtac attccggtgg cacaatcaat tttgttaaca
    34381 atactttaaa cccaactgta cagagtttaa cgattaaaag ccctaacgtt cttcttggcc
    34441 cttccggaac aattaaacgc aatggtgcag gaacaattgc ttcacgcggt gcattagatt
    34501 ttgcacgtac aggaactaca ttatttactc gtttaaatgg ccatactgta gatcacactg
    34561 ttcttactgt tgtcaatggt acaactttag aagtaatttc cggagatgtg cagttgactt
    34621 cttatgttga agaatttgcc ggacaaacgt taccgatgct ttatgtttca tcaggtgcta
    34681 cttttacatt aagaaataat agctctgttt atactacagg aaattttcta aaaagtaccg
    34741 tgcaaggtgc tagtggatca agaatacgta ttcaacatac caatggattc tattcaggat
    34801 ttagttctgc tgctatcaat tcaagcggca atatgggatt tctactttct tcgtccagta
    34861 cagtagaata tttcggtgat gacaatcaga ttattaccgg aattggaatg ggtaatgctg
    34921 ttggcggcaa ccaaaaatat ggtgtgctcg aaataaactt tggtggtgac ccaaatacag
    34981 agtatgttta tcctacaagc aatggcacag ttaacgtcag aaatcaatta aaattaactg
    35041 caggtcagtt gcgtcttgat gatgataatg atcctaccaa tggaggaaga acaattataa
    35101 ttgaaaatag ccctaacacc tctatcacca gggtagccgg atacatacga gccgaaactt
    35161 atgatggcgc aagtaatgtc aaatggatgt ttgctgctga caatacacca cacatattcc
    35221 catttggatt agacccaaca acctacatac ctttaaccgt tacttcaaca ggaggtaatg
    35281 ctggattcat aacagcatca acatttaaaa caagcccggc aaaccttcct tatcctcctg
    35341 gcgtaggtca cgttaattcc gttgctaccg gtgctgacaa tagcgcagat tgtgtagatc
    35401 gtttttggaa aataaatcaa tcaggcacaa gcactacatt caatgcagtt ttctctgctc
    35461 ctgttgctga atttccagca atagcaggtc catataaagc acaacattat aattacgtag
    35521 gtggattctg ggataatcca tttcagggcg ctcaggctta tgcctctgca ggtccaataa
    35581 gaacagtaac agctacagga gtttccgtta caaatgattg gtgggtatta gtaaatcaaa
    35641 acacgccact acctgtagaa ctactttcct ttgatgctaa ttgtgtaaag aaccatcatc
    35701 agattaaatg gacaactgct tctgaactta ataatgactt cttcactctt gagaaaagtg
    35761 cagatggcac tgagtttaca ttattccgta aaattgaagg cagtggcaca gtaaatgatt
    35821 ctaagaacta tgaagtttca accgatgatg gcattgctgc atactataga ctcagacaat
    35881 atgattttaa tggaaccatg catgatttaa aaacaattta tgcaccatca tgtcaaaatg
    35941 atgagtttaa attattgtct gtaaaccaag ttgaagatat tgttaactct atcgtttcaa
    36001 gtcccgataa tataaaagca gaaataactt tattcgactt aaaaggagct ttaattttta
    36061 aagaagaagt ttcgctttca acgggagtga attcagttcg cctacctata aaccttgcaa
    36121 agggattata ttacttccag attattaacg acaatcaggt aacccaaggc agaaaattct
    36181 tggtcaacta agaataagta caatttaata aaaacccatg tcaattcatg ggttttttta
    36241 ttgcatttca aagtatttcc tatttttagc cttgcaaaca ttttagcatg actttgatag
    36301 gcagaataat taaggcaagt attgatctca gaggaaaaat tgagattgta cccttttctc
    36361 cttttatttc tcagaaacgt gtactgaaaa agttgataag aaaagcccag tttaccatgt
    36421 ttggtgaagc ttacaacttt caaggtatgc tggggaataa aaattttgta actaaattca
    36481 gggcagcagt tccagttcat gactacaatt caattttttc aaattggtgg aataaaagtt
    36541 tacatgatca ggaaaatgtt tgttggcccg gcaaggttcg ttactttgct ttaagttccg
    36601 gcacttcggg ttcatcaagc aaacatattc ccgtaacaaa ggatatgtcg aaagccattc
    36661 aaaaagccag cttaaaacag attctttctt tatctaaata cgacctgcct ttctcaactt
    36721 ttgaaaaagg tatattaatg ttaggcggta gtacacactt acatcagcaa ggcagttatt
    36781 ttgaaggcga tcttagtgga atcacagcaa gtcagattcc attttggttt caacattttt
    36841 ataagcctgg cagaaaaata tccagagaaa aagattggca tgcaaaactt gatgaaataa
    36901 cgcacaaggc aaaagattgg gacataggta ttattgtagg tgttcctgcc tgggttcaac
    36961 ttttgattga aaaaatactt gaccattaca agcttaaaaa tattcacgaa ctgtggccca
    37021 accttgaaat ttatgttcac ggtggtgtct ccttcgagcc ttataaaaaa ggatttagca
    37081 aactcttagg gaaacaaatt tattacatag aaacctattt agcatcagaa ggatttattg
    37141 cctatcagga tgagccaaac acaagaggta tgagacttct cgttaacaat ggaatatttt
    37201 atgagttcat accatttaac gaagataatt ttgatgctga tggtgaatta aaacacaaac
    37261 cgaaaacttt attactaaac gaagttaagg ccaatgaacc ttatgcgata ttattaacaa
    37321 cttgttccgg tgcatggcgt tatttaattg gtgatgtaat tacatttact aatttagaaa
    37381 aatgtgaaat tcagattatc ggtagaacaa aacactatct gagtttatgt ggtgaacacc
    37441 tgtctgttga caacatgaac agagcaattg aacttgtttc tgatgaattg aatattgata
    37501 tccctgaatt tggtgttgca ggtatcccct atcaatcttt attcgcacac aaatggtata
    37561 ttggttgtga tacttataat gactcagaga atctgaaaaa gcttattgac gaaaaactga
    37621 aaattcttaa tgatgattat agggttgaga gaagaagtgc attgaaagaa atttttgttg
    37681 aaataattcc aacatcttgg ttttacaaat ggatggagct acaaggcaaa attggtgcac
    37741 aaaataaatt tcccagagta atgaaaaata aagtttttga ggattgggaa aagtttgttg
    37801 catcacaaca taagaacata aatcaagcag tttaattttt tataaactac aattttgatt
    37861 gagcctgtaa taagtggatt tctgttaggt tgctttctgg caattcttgc cggaccggtt
    37921 ttttttctca tcataaacat cagcttacgc aaaggcttta gctatgcaag tagagtggca
    37981 ataggtgtac tcattagtga tacacttttt attttcatag cctactttgg aagtaatttt
    38041 attctactac tcaacaatca cagatttata gcaggaataa tttcaggaat tttgcttatc
    38101 ggttttggaa tagctttaat acttaaaaga aatagtattc ctgctgaagc tataacagta
    38161 aacgaaaaaa cagatagcaa gagtctttat attttaaaag gatttatgat taattccatg
    38221 aatccttctg tgctattttt ctggattggt gtttcgggaa cggtttctgt aaaagagcac
    38281 tacaccattc atcacctttt gttgttttac ggttctattt taatcacact cttctctttt
    38341 gatctattta aagcctacat tgcacataaa ttaaaatcta tattgacaat aaatttactc
    38401 acctggctca ataggattac aggcatggca ttacttggaa ttggcttata tacgttgata
    38461 gttgcctgtt ggcaggccta tgcaaaataa aacacaacca ttataagcat caaatatcaa
    38521 aaattacact tattccttaa ctatcaatta agagttctta ccaataaaaa accacctatt
    38581 gtagcagata taccaatagc caaacttaga aaaacatata cagtcatcat tccataataa
    38641 ccattacgca acatcataaa cgactccatt gaaaaagctg aaaacgttgt aaacccacca
    38701 agtattcctg tagcaataaa taagcgccac tctactgcca ttttaccttg ttcggcaaag
    38761 ctatacacaa cgccaattaa aaagcacccg ataatattta caagcaaggt gccgaccgga
    38821 aattctgaaa ctgtcttgga ttgaataaaa agtgagagca tgtatcgaat catgccgcct
    38881 ataaaactac caaaacctat tgttaaaatt gtcctcatat taaatcacta aaagcataat
    38941 ctatatgcct atattaaggc tgattgaata atttattata tacaaaataa tacaaaacta
    39001 atgctactgc caaacctgta atccatccac ctaaaatatc aaacggataa tgcaccccta
    39061 aatatactct gctatatgaa actaataaga catagattgc caatagagca ataactattc
    39121 tttctttttt aaaaactgac aatacaagaa acgcaaacaa agccattgta tttgttgcat
    39181 gagatgatac aaaaccatat ttgcctccac atttattatt aactgtatgt actacaccct
    39241 gaatattcag attatgacaa ggtctataac gcattacatt ttcttttatc aaaacagaaa
    39301 gctgatcact gcagataatt aatataacaa taaacaacag tatgctccat gttttatttt
    39361 ttttcttctt tattagcaaa aaaagtaaaa tcagataaac cggaatccat acaaagcgat
    39421 tggtaatagc aaagaaaaaa gaatccatgg ccggactatg tattgagttc agccataaga
    39481 ataaatttgt atcaaattct tttatctgct caatcatgaa ttaagttctt tccacaaaac
    39541 atctttaagt ttatcaatat taaaattcgc aacagacgaa ataaaaacta caggcacttt
    39601 gcctcctccg ggatttttgt cattaggtac ttcttttttc aattctttca acaactcctc
    39661 atccaacata tccgattttg tgatggcaat aacccttctt ttatccaaca attcaggatt
    39721 atgctgacgt agttcgttct taagtatttt atactcttct ttaatacttt ttgcatctgc
    39781 agcaatcaaa aataacagta ttgaattacg ctcaatatgt cttaaaaatc ttaaccccaa
    39841 cccttttccg gtatgtgcac cttcaataat tcctggaatg tcagccatca caaacgacct
    39901 gtcatcatga taatagacca tgcccagatt aggaactaag gttgtaaatg gatagtctgc
    39961 aatttctggt ttggcagcag acacaacaga aagtagtgta gattttcctg catttggaaa
    40021 accaaccaac ccaacatctg ccataagttt taactccaaa attttccatt cttcaatacc
    40081 ttcgccaccg ggttgcgcat gacgtggagt ttgtaatgta gatgttttga aaaaagcatt
    40141 tcccttgcct cctctccctc ctcttgccaa tatcacttcc tgcccatctt cttctatttc
    40201 aaacaaaaat ttattggttt caaaatctct tgcaatggtt cctattggta cttcaagaat
    40261 ctcatcttta ccatcagcac ctgtacgtaa tgcattacct cccttttgtc ctgcatctgc
    40321 aagtatatgt ttacgatatt tcaaatgcaa taaagtccaa agttgtttgt taccccgcac
    40381 aataatatgt cctcctcgtc caccatcgcc cccgtcaggt ccaccttttg cggtcttttt
    40441 atctctgtga aaatgtacat agcccggacc tcctgctcct gaacggcaac aaattttaac
    40501 ataatcaaca aaatttttct ccatttaatt taactgtttt aaactgttct attttcaatt
    40561 tacagaggaa aaaattaatg agctacaatt acctccaaat ccaaatgaat taagaagact
    40621 atgctttatc ttttctttct tgaaattcaa gttaggtaca agaccggttt cctcatcagg
    40681 agtttcgaaa tttctgcttg gataaacatg gccatctctt actccaatta cagccagcac
    40741 actttctact gctgatgcag cacccaaagt atgtcctaaa atagatttta aagaacatac
    40801 cggtggcagt gattctccaa aaattctttt tatagataat ccttctactc tgtcattatt
    40861 gggtgtacct gtgccgtggg caattacact gttaatttct ttaggcatca atcccgcaga
    40921 ttccaacgca ccacgcattg ctgccattgg gccaaaacct tcatcactca aagctgaagg
    40981 atgaaaagca tcatttgaat tagcataacc tttgatataa gcaagtactt tttcttcttt
    41041 cgctgtatct gacgactcga gtataagaaa acctgcaccc tcaccaagat ttaatccatc
    41101 acgattatta tcaaatggtc tgcaacttct tgttgacaat atattcagtg cattaaatcc
    41161 atttattgta aacttagaaa gacaatctac accaccaaca ataactcttt tagcacgttt
    41221 attttttaat agcctcattc catatatcaa agcatttgcc ccggacgaac aagctgtgtt
    41281 aatagtattt atcacacctt ttgctccggt aagttgctgc aaataaattc caattgaagc
    41341 acaatcgtac aatgaaagat aatcagaacc tttagaaaga ttatttgaat ctgcatataa
    41401 ttcatcagta aggtacatgc caccaacagt acttgaaaca actattgacg tttccgtatt
    41461 tttcaaatca tctatatgta atgcactatt atttagtgcc tcattaaata ctttatgggc
    41521 cagtaattgc gtgcgactga caactttatt tctaattgat aattcatcgg ctaactgcat
    41581 gtcagacaat tttacttctc caaatggtat catcgaagca tatctgcctt ttacatattt
    41641 tggaatacct attcctgttt ttccactttg aatagcaagg atggtttcat ttgcattgtt
    41701 tccgacagct gtaaccaaac caactcctgt tatgcaaaca ttctccacaa tacaaaaaat
    41761 tattttgcag acttatgctg aataatataa tcagcaattg tattgacatc aatcaaaatt
    41821 tttcttcctt ctttggggtc agtaattttt ataccatact cacgctctaa aagaacaata
    41881 agttcaatgg aatctattga atctaaccct aatcctcctt ctccaaacag aggttggtca
    41941 tcttttatac tttctggtgt taagtcaagc aggtttaaat atttaataat atgtttcttt
    42001 aactctggct taatttcttc ttttgtcata ataaattttt ccgttttaat ccgataaagg
    42061 caataagctg aaggattgct gtcagcaaaa gcaaaggtaa aacatttagc agtagcttat
    42121 gaattggtga tatttctaat attgctgaat agtaagcttt cattgcccaa ctgaatggtg
    42181 aaacattagc aattaactta aaaacttcag gcattgcaaa taatggaaca aaaatacctc
    42241 ctactgctgc cagtataaca acagaagcag caccaaagcc attagcctgc tcaaccgtgt
    42301 cagaataaac accaatacac aaggcaaagc ttactgcaca caacccacaa cataatgtag
    42361 ccaaaaacaa tggcaataac cctgatgaaa ttaccaatgg cggcaattgc aacataggaa
    42421 atattagaat tcctatcgaa aacaagacca acgtttgtaa aactgtcaca atccaaaaga
    42481 ccacttgttt agcaatcaac atttgggtaa ttgatatggg caaagttttt attcgtaaaa
    42541 aacttccgct tcttttttct ctgacaatgt ttccacctaa cgatatcaca gtaaaaaaca
    42601 tggcaaatac tgtccatgca ggtatgttat gttgtgttgc atttgggttt gcaacactat
    42661 tttctttaat taatgactcg cttagcaaca atggctttgg taatatctta ctcaatgcag
    42721 cagaatcagg ttctgattga ttgacatcat aaaaaagttg tttcaataac atttcattct
    42781 gaatagccat gactacagat aaaaccgttg acttaattga aaaacgatat gactcctgaa
    42841 gtaccggatc aaaactcagt tctatatttt ttactcctat tattcctttt gttgaaactg
    42901 actcaagcat accaaacatt tgcaatgact gttctgcctt tgcttttact tcctgatcaa
    42961 gtgtataagc ataccctttg ggtatagtaa ctactacaat tgactcttta tcttctgata
    43021 actttttgct tgatatctct ttagtattaa ttttcaaatc aaacatgccg gtttgctcaa
    43081 tgcctttaca aaaattctga aaaaacagac tattagaatc attattaatt aacattaaat
    43141 caactttgtt ttcgttgaca atcttaaatg cactgttttg taaaacagtc ataagtaatg
    43201 caagtacaag aggcattaaa aacatcaaaa gcagtccggt cttatctctc gaaaggactt
    43261 taaactcttt aataattgat gctactaatg ggctcattag tctctgtaat tattacctgt
    43321 taatttaata aacacttctt caagtgactt acattgattc tgtatcagca aatttgctat
    43381 tgtatcaatt gctataactt ttcctttgtc aattaatgca atacgagaac aaaattcttc
    43441 tgcctcttta agcaaatgtg aagtataaat tattgttgtg ccctcagaat ttattttttt
    43501 cagataactc attatagcca atctgctctg aacatccact cctacagttg gctcatcaag
    43561 aatcagcaat tgtggtttat gtaacaaacc aattgcaatg tttaatctac gtttcattcc
    43621 gcctgaaaat tctttgacat acctttctgc aacattcatt aatccaaatt cctcaagcaa
    43681 ttctttcgct ctgttgtcca aagcttgttc cttcaatcca tacatggcac caaaaaaaat
    43741 cagattctct atagcgctca actcttcata gaatgcaaaa tcttgtggca catatcctat
    43801 tatctgatta acattttttc gttgggtaga tgcatctttc cctaaaatag aaatcacccc
    43861 caacctacat gttaaaatac ctgtgaccaa tgataacaat gttgttttac ctgcaccatt
    43921 tggtccgaac acaccaaaaa cttctccctt accgacatgc aggttaatgt tagacaacac
    43981 agcatgatcg ctatttttat aagtataaga taatgatgat acagtgagta catccataaa
    44041 gtttatgctt tcacagtttt acttttgaca aatcagtaaa tgcatttttt tccacttctg
    44101 caatctgcat caaccggtca tgcatattca caaagtcagc cctgtctgta agccggcctt
    44161 taaatattcc tgctgactgt attgctgaat cgcgccacat atcaccggcc tttgtaaact
    44221 gttctgctat tttaatgata ctgtcatttt ttaaataagc atgagactgc tgcaaaaatg
    44281 ctgcatagat ataccgaaat ccaccaccac ctgtacctat ttcttcttgc atccttacca
    44341 actgcgccaa atacaaacct gtggtttttg ctcctaaacg atcatgccat tttaaaattt
    44401 tttttgcaac atattttatc ccgcttacac cggcaattgg tccgggtata taaagcatat
    44461 ctcttatgtt ttgctttata ccacgtttaa tcgcataaac gattttctcg tcagaaacaa
    44521 tattttcaat ttgtggataa tataattgtc ccttaggtgc caaaggtcct ttagcaaaac
    44581 gcactctgtt caaatcaaac tccgttaaag aggttgtgtc ctccatcacc ggatcgctga
    44641 ttaaatacct gtcatctgtc ttaccataaa caacaagatt gtgagcatta aaatggaaac
    44701 gatattcctt tggaaaatat gtcagatagt aaactccaac ctggcatccc accggttggt
    44761 tttttataac acaatcatca cgtattcttt gcgcctccga ttctgaacgg aacttttttc
    44821 ttatcacctt tattcctaaa cgactgcatg ttcttttaaa aattaatccc ggcatagttc
    44881 tatatgagaa tgccggagca ttattaactt taagaaatgg aatataaata aaaaataatc
    44941 ctgaacccat accaaacact aatggctcag ttagtctcaa tcctgaatag ttcaaaagat
    45001 taacagttac accattttcg caatgtgccg attgctggtg attaaaattt atctgcatta
    45061 aatttttctg tttttaaatc ttcaacgtct atttcaaata ttaaagcata tttcaacaac
    45121 actttattgt cgagctttac aaaaacagaa ggctttaaan nnnnnnnnnn nnnnnnnnnn
    45181 nnnnnnnnac ttccagaatc ctgtataagc acttaaaata ccaatatcca ttaatcgcaa
    45241 ttccatataa tacaaaattg ggcttacttc gcgattcttt acttgactat atgctgtaac
    45301 aattctttgt tcaatatcct gccaggctaa attcaatgca ttggttttaa catcccatcc
    45361 tgtactgttt gctgtagtat attgaccatc ttcgccaaca gcatagcaca actccttaat
    45421 ataattgact aatgcactat gatcctgcgg aatatctttc ttattcatta aacaacagtt
    45481 aatatggcaa aaacatacga aaaacgtgcg ctctcaggta ctgctaatac aattttttct
    45541 ccttgtttta gccgatttgt tcgcattaat tcttcaagca tcagataaat agatcctgct
    45601 ccaacatttc caacttcaga taagttggta aaccatctat tcattggtat aaccaaacct
    45661 ttcgaacgaa aaccttcgtc cattttatct ctgaaaaatt cacttgaaat atgtggtaaa
    45721 aaccaatcta ttttatctcc tggcaaatta tgtgctttaa gcattgatat taatttatct
    45781 gtacccaaat caacaatata tttgcttaac aactttacat cttgttttat gttcaataca
    45841 gaattggtca caatctctga gggtgacata tccatataac tcacaaatga tccatcatcc
    45901 attttatcgg ctgcagaata catgcatggc tctactaaat gagcatagga cacgccttct
    45961 atggcatcaa ttcgcaatga caatccattc tctttctttt tattttcaat taatgccgca
    46021 gcagctccat cactcaacat ccatcttaaa aattctttat cgaatgatat atatggattt
    46081 tcctcaatct gtgaaagttt ttttgcttcc tcctcaaacg actgatgatg cataattctg
    46141 gaagtacgct ccgaaccggc aacgactgct ttttttgatt gtcctgcttt tactgccagc
    46201 catatatatt ttaaagcatg cattccggaa cagcaattac ctgttggcga caccacctcc
    46261 atgtcttttg cttcagtaag ccagccatga accatcacag cgtgcgatgg catccattgg
    46321 tcaggtgatg atgtgccgca gctcaataaa tccacttctt taatttcttc ctgacgatta
    46381 gcaaaaagtt cttttattgc gagtgctgtc atttcagaat tggtatgcgt tgctttccca
    46441 tttttgtcta atgcataata acgacacttt atgccattat tacgtaaaac aatgcgcttt
    46501 gattttgctg aatgttcatt tatcaagcca atgtattgct ccatctcatc attcaaaaca
    46561 ggcttattgg gcaaaaacct tgatagtcgg gtaatgtaaa cttcgtgtaa cataacttag
    46621 ggctgcaaat ttacaccttc atggaaatct ttttgcctgt ttaatgattt attaaataat
    46681 ggtagtatta ttattgtaaa taacagaatt acaatcggtg aaagtaaaaa taaagcaaat
    46741 aaaagatata ttctaaaaat attta
//
LOCUS       DAOOOI010000011        45235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_133_length_45235_cov_45.326073, whole genome shotgun sequence.
ACCESSION   DAOOOI010000011 DAOOOI010000000
VERSION     DAOOOI010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 45235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45235
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133_length_45235_cov_45.326073"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..3209
                     /locus_tag="PKX59_03245"
     CDS             72..3209
                     /locus_tag="PKX59_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89914.1"
                     /translation="MKRPFNIFPSIGYRNSQFQIVSSVDNLKIDIYDQDKVVKSFEAN
                     SKNPTLLTSLGTTGKLIAKCNFNNIVFQQELEIKEAFRLGSSEFKKAFVFDDTEYSFF
                     LMKDRLLLYDEKKKILLTENHYSPTEIYKINGTNFLFVTRVGNSASGIINLGVYNTEN
                     FSMVGELLNDYREIKILADNNKAWLYNIKSKTIHCYELAHQTNKYLTELKSFENFNDY
                     FLDDANLNIYINHNEVLKISSLNNLHKYFEIPKNENNAIDKLGNVFTLENKTLKCTSL
                     HTTYSETVNPDFEVNLETDNFIHIGKHLKSEGELTDLIRKAEEIREEVISSLPDAKTY
                     HYHALSESQKISEIYTTHKIYPTKNGVFIIQKRIKRDFNGVTLRKCQATWTATPYKIE
                     NTEITLLLFNSEKVDILIDKAPNLTVYEYLKDMLVVSHQSTRILFSGRNKVTLNNEDS
                     IELFTTNEIAYFLIKSKERYSLYRSTNPNNSILEQIEILNPDLFKKHQIIWYRGKEKN
                     ISNTKYLNAFDLKNCTRILIDEQKVQHSLFKDALDFKFFEKYALSSNQIVFNPATLEI
                     KDAFIGNIESHSKQLNKIVSHRTNKIYLSKFNPQIAKYEVCEISIDDNKYRESYLSPN
                     GQFLVLQDESNKYSFYDIEKGETINFISGNFLAFRNDGSLIVEQDGTKAVKIIDPKTF
                     QDITPANYHHYRFMSPDGKLYAQVSSKVRYFHKLNGKEIKAVEVARIRQDLDEPSIFL
                     KEDEREKAKIKVSENRKDFYDSFKNQFEEIGIEDYNKITSQSVVKVDKFTEIGITGTN
                     IITEVHFPDDLAFYNYSAFSYDNNYFGYVGKPSSNGLIHLFKIDFDEVNSKLLVSDTY
                     LSRYPRYASWVCGFSKTGYFATYDSTPNTYILFVDDSLFDSKTTEVELRQNIYKSKTN
                     IYHTYNKWNEIKGKNFLCFSPTGDYLALSEQGYEPLTLGGYGHQESNVVHIAKTESGA
                     IIDSFTGHGDKIKDDTRKKVTFVAFSDDESRIMTLSSDGVVIIRDINISDYEQNDRMP
                     AANKRLAAMAGDVVN"
     gene            3692..4279
                     /locus_tag="PKX59_03250"
     CDS             3692..4279
                     /locus_tag="PKX59_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOZ89915.1"
                     /translation="MQQFTDRQIEIMEAATNRISKYGIQNLTIKTLAEDIGLSEPALY
                     RHFKSKNEILLSLLEYFKTEMKTRIQSISFKPTDTTADELRAIFNSQLQTFTNKPAIV
                     SVIFAESIFHFDESLSNKVAEIMDMMQDYVKTNITKGQEKGQYNKLIGSSTLTTIVIG
                     GMRMTVLKWKLSGHKSNLVKDGQTVLDGILKMIEK"
     gene            4284..4844
                     /locus_tag="PKX59_03255"
     CDS             4284..4844
                     /locus_tag="PKX59_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019977001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOZ89916.1"
                     /translation="MNTQKKFTPFHRLLHWIMAIAMPILFITGFLRMYWMNKNNIVAV
                     IESKTSAIPKEQMTDIAKAIREPMWQWHELFAHVMIFAFLARIIYMLVKGIRFPNPFK
                     SKQPLKERLQGFTYIYLYLFVFISAFTGICIEKDLLSTYHEQIEAVHKWGIYWFPIFI
                     LLHLVGIVLAELSNKKGITSKMIGGD"
     gene            4887..6212
                     /locus_tag="PKX59_03260"
     CDS             4887..6212
                     /locus_tag="PKX59_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOZ89917.1"
                     /translation="MLVNTRKLIFFSIVIIGWNTVRAQEVWTLKQCIDTAKVHNKTLQ
                     INRNNISISEQRIKEAQANLIPKITANTDYKYFTELPTQLMPMNALNPQAPEGQFRDL
                     QFGVPHNINANIQLAMPLYNPQVYGAIQNTKIANELTQLQYQKSEEQVLYDITTLYYN
                     AQILKHQLEFLETNLENTNKLLKNIQLLKEQLLAKGTDVNKVQLQAEQLATQKENVNN
                     KYISILNALKLNMGIALETKISVESAISFQTTLEYTSKTTLDIQLIQTQNRFLNAELS
                     TLNKSRFLPSLNLIASYGTTGFGYDKTPNDFLKFYPISFAGVQLSYPLFNGAVTLRKI
                     NQKKLEINNNELQVQLMNDKNKMEIENSTRQKNVAQQTVLNTEKQIALAQTIYDQTII
                     QQKQGTASLTDVLLADNALREAQQNYLSAIIDYLKADLELKKVTGSIGN"
     gene            6276..7328
                     /locus_tag="PKX59_03265"
     CDS             6276..7328
                     /locus_tag="PKX59_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ89918.1"
                     /translation="MKKIIWIIAGVAVVGLMMFKLLSNKKTTENRVYQYDKEKPITVS
                     VDTIRLQNIVDAGNYTGTFEPNKETKISADIQGKINAVLVDVGDIVQKGQTLIQLDNS
                     LLKLQLQTVEVQIEGLEDDVKRYTILTEADAVQGVQLEKSKLGLKSAKIQRATLLEQI
                     SKTSIKAPFNGIVTAKLNEEGGFAAPGIPLLQITDINTLRFTVNVPEKDLVQFQTNKT
                     YNITSDVYPEIPLFGKIIMTGSKANMGNSFPVQFQVENTKQLSIKSGMFGKVNLSSTS
                     QEQGILIPSSAIIEVDGVAKVYIVKNGKAVLQTITTSKNIGNKTLISSGLKENDIIVT
                     NGFINLFENANVLIKN"
     gene            7368..7724
                     /locus_tag="PKX59_03270"
     CDS             7368..7724
                     /locus_tag="PKX59_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010523003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOZ89919.1"
                     /translation="MKENIVQQKSFAFAIRIVELYKYLQSEKKEFVLSKQVLRSGTSI
                     GANIEESIGGASEKDFLHKLTISYKEARETIYWLKLLHATQYISEKEFNSLHNDAEEI
                     CKILAKIQITLKSRNS"
     gene            7748..10840
                     /locus_tag="PKX59_03275"
     CDS             7748..10840
                     /locus_tag="PKX59_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOZ89920.1"
                     /translation="MNITEISIKRPSLIIVLFSVFALLGIIGFKNLSYELMPDFNQPV
                     VVIKTVYPGAEPNEVETSVSRKIEDALSNLEGVDYLVTKSLPNASIIIANLKYGTDLD
                     KTMQDAQRYIDNIKKDLPKDIQNPVMSKVSPNDLPIMSVSATSDLNPTEFYQKMKDDY
                     LPQIQKLKGVAEITVLGGEEREIQVKVDKDKLKLYKISLYQVVEAINRSGIDLPAGKV
                     QTDQENSSVRLVGKFNDLSSIQNVQVAMPMPGSPVYVKDIAEVVDGIKETSSISRYNG
                     KNGIGLLIKKQSDGNAVDVSKLIKEQFQTIEKHNASNNVKFVITDDSTDNTIAAVNSV
                     VFDLILAVILVSIVMLLFLRSYRNSLIVAVAIPTSLITAFGTMWLLGYTLNLMTLLAM
                     SLVIGVLVDDATVVLENIQRHLDMGKDKRKAAMDGRMEIGFSALSITLVDVVVFVPIL
                     FLQVFVADMLKQFSVVIIVTVLTSLLVGFTLTPWLASRIGEKENLQPSNFFNRFLLWF
                     EHQLDKFIAWYGGKLDWVLRHKLLFTGFVIFLFIGTAVMMKQGIIGKELIATGDQGKF
                     LLGLEFDKNTSIQQNNLISEKIETYILQQPEVATLFSNVGGPSTGIGSLGVGSANKSE
                     FTIQLKPKKETNNLSTEKFMTDLREDLQTKFSGINYSISGLGLIPKTAPIEITLSGSE
                     LNRVMQTAQNLKMVIEKIPGADNVRLSVEAGSPELKVIPDRDKMQRLGLNTAYVGMNL
                     RTAFSGNDDASLTENGTEYPVRIWLDKFSRQNYDDINNLNIVNPMGIPIEVSQFASIE
                     KDNSPSLLERKDRQPAVTLTADALGRPSGTVADEVVAYVKNNPLPNGIEMTWGSDIKR
                     QNDSFGALGSVLIISFILIYLIMVALYDSYIYPFVALFAIPVAAIGAFLALNLSLSHL
                     SLFALLGLIMLMGLVTKNSILIVDFTNQLKAEGKPYRQAIIEASKGRMRPILMTTLSM
                     AIGMLPIALATGTSAEWKNGLAWVIIGGLLSSLILTVFLVPMVYYGVDRVKEKLKIKN
                     "
     gene            10887..11327
                     /locus_tag="PKX59_03280"
     CDS             10887..11327
                     /locus_tag="PKX59_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HOZ89921.1"
                     /translation="MRVVFFVVMSVFVLSVFAQAKAEEYSLTIEVESLRNSKGILQVA
                     LYNKDGSIPDEYYKNYFKIETAQITNGKSKIIFKNLPKGKYAVNILHDENNNGKIDKG
                     LIFPKEGIGFSNYSSIGLTNRPNFKKASFDLFQDKEIKVKVIYM"
     gene            11704..12750
                     /locus_tag="PKX59_03285"
     CDS             11704..12750
                     /locus_tag="PKX59_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89922.1"
                     /translation="MKRLLLILGLFISSSGMAQLTGLTLTGNPESLNGATWTYQQTIN
                     GTSYDLTGILFKPVGSGVFPAVIINHGTGGNVNGYSKNVAKKMVQWNYTCIAVNLCHS
                     AGAPIGSPGDSSLINFGASTNNYLRDMKCWDILASLNYVDTNCIMAFGHSRGAYLTTG
                     LVATYPDKFRAAGHTAGGVSPQLGFSAPTTMLAAQISCPYIIHHGDADNTVPIEMDSL
                     LNSVFNSTGIPHQFYQYSGYTHSSISMDSLMFARTQQWFALHGCANTTSINQQISSEM
                     ISIFPNPTSDFIQFKTEKRIDVSLFDVSGKLLLSKIVMPNEQINVSQLNNGIYFITIN
                     LYRKMFHHKIIIQK"
     gene            12774..14072
                     /locus_tag="PKX59_03290"
     CDS             12774..14072
                     /locus_tag="PKX59_03290"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89923.1"
                     /translation="MFVVLDTQAQFIGHNGVYFLGTANMPISTTSFNDPNISGVVVRF
                     RWNDLEPSPGNFNWAFIDGEIAKAVTYNKKISLQPLGIPNWLGSIGAQQYYTIQDNPS
                     SPNFGQITTGVIPWDSVYVSRFKIFLQNLAIKYAGNPVVTYVNVIGVNFSRNLPDTVL
                     TDTVTITKQAFWSVYNYNADSLGALINEMTDYYMSVFPSTPLWCSVDYVTFQPNASGQ
                     ARNYLASIYCNYGIINYPDRFGLFREDVSGCNPNLSNIANTSHWYLMQQNHCRTGAQM
                     LWSVQDGPSRMNQCGILPNTKAMVLDSAVNNGLALGMRYLEIYGSDITDTSLASTIQQ
                     ANNDLIAKGLLCNSSTGLNEPISENTFSIYPNPAREYLTINFDPNQKTQIQIFNSMGM
                     LFKEFSVTTSTQLDISDLPNGLFFVRLKNYSQPIKKFMKQ"
     gene            complement(14364..16127)
                     /locus_tag="PKX59_03295"
     CDS             complement(14364..16127)
                     /locus_tag="PKX59_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89924.1"
                     /translation="MQKYALKVRQRLADYISLPDKTKVTVMQQQKCLNFFILFILSCL
                     FASDIEAQQYKFAKRVGGTQFENAMGIALDSTGNIIVSGYFSGTADFDPSTSNYSLTS
                     NGGNDIFLARYDSAGNFIWAINVGGAGEEFNYSDPAVDEYGNIYLCGVFNTNTNFNPK
                     GIAVMATNRGQQDGFIAKYDSTGLLQWVRGVGGVLNDDVYRIDYKDYLVLFAGSFTDS
                     SFVDDGLTVTPIYGSGNADVVFGKFNPNGNLFWLNTLKGSGEDHSYNITAGINGKTYL
                     TGTFEDTLIFDYVSVSPDTLFADGLAAFTASFSNMGNYYWSFPIENAYPFGLKVDHNG
                     DILTCGQFSGIADFDPDADTMALMAQGSFDGYFAKYTNGGSYDFAKRIGGNGADICYT
                     IAELSNSSILVSGYFFNTADFDPDVTVASLNSNGFADMYLAHYDSMGNYLSAFGCGSP
                     AFEFCRNMTCNGIDEVFLCGGFEQVVDFNPAPAINTLVSAGSRDGYFAKYAFPTTSLN
                     SISADNLMIYPNPFISQINISGDINQTQLILTDVLGNTIYKTKTNSQTTINTEKLNPG
                     IYFLTVVSGENVSTRKLLKVK"
     gene            16133..17386
                     /locus_tag="PKX59_03300"
     CDS             16133..17386
                     /locus_tag="PKX59_03300"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOZ89925.1"
                     /translation="MDHSTKTFALSNGLQIVLTTLSNTEVSHCALLIKAGTRNEQIQQ
                     EGLAHFIEHMLFKGTRKRKSFHILNRLEVVGGELNAFTSKEETCIHASVTTPHLERAL
                     ELISDITFNSVFNLKEIEKEKEVVCDEIRSYLDNPGEQIFDDFESLVFKKNPLGNPIL
                     GTEQSVKSFKQKDLIAFTRKFYIPSNMVLSIATSLNAEKVIRQLEHHFGKYRLAKVGE
                     RPKPFKKYTAEKSSVKKHSAQCHFIMGTLAPSLYQADRMTVALLNNILGGPGMNSKLN
                     LGVREKYGYTYAIESGFNSYTDTGMFHVYLATDKKFLNKSIDLAVVEMKKLTDNKIGS
                     LQLSQYKQQLKGQLALAKENKSNVVISNARSLLNFKKVFTLEDTYKKIEAVTSSQLTE
                     AAAKYLNAKSRSYSSLLYESVGHEQ"
     gene            17376..20129
                     /locus_tag="PKX59_03305"
     CDS             17376..20129
                     /locus_tag="PKX59_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOZ89926.1"
                     /translation="MNNKTDSFLTRIVDELIATVDGNNFADYTVVFPTRRAALLFKLQ
                     LAKKLNKAVLLPSVLSISDFVEHYMPLRVAEKKEVLLDLFAVYKPFFPDYDFTSFAPW
                     GEMILGDFEEINLYLVNYKDLFADLTSYHEIESVFQDDDEFKEQLKQFFELFSTDKTS
                     TIKQNFLYAWQALPKLYEKLNEVLYKNNKTTAGKAMQQLALSPETYLKNLDCSKTIIA
                     GFYALAPAEQKLFEYILNGKGKIFWDADEYYTKNTTQEAGLFFRKNFLTQNSFKWQET
                     YFKDIPKNINVTGVPLITGQFKFLADRLKTLIAQNKLNTDKTAIVLPDEKLLPLLLQS
                     LPEEIEHVNITMGFPLKETHLYRFIETLNVLLNQISETSKQKFVSTFALGNYLAHPYA
                     SLLKHDIRNLNDAGISIPLNELPENISQFLKSQITSPIFEGYIQLLTMLHDAMEPKHH
                     EKEIAGFVLNELLELRTMVIPYTEILNEQALQMLISEILDTTRIPFSGEPVKGLQVMG
                     LLETRTLDFENIFFLNVNEGSVPKSNRHHSFIPFTIRKSFGLPLLDEQDAITAYHFWR
                     LMQRAYEIDLIYNTEVNEFASGERSRFLLQLFYEMKLHFSNWQVSHQIITTPLYRYNY
                     ITPGVKRDEQLTNHLLSMVAENKLRFSASSLNSFIHCSLQYYFKEIKKIREPDDNGEE
                     IDAAVFGKILHDAVSKIYAQIIDVEFDKDNAAQLKKSISAFVKASIAQEYDKHYQGTG
                     YTVIIEKIISEYIERIIAADLKSETFSPHELETEKVVVCDIGNGINVKIKGIFDRIDK
                     VSDGYRIVDYKTGSDVVKNIKKPDELTSEPKYKVNFQLLLYCWLFLKSHSNEKVNAGI
                     YPLRRMEAGIEMLGQQPLTDHSKFEELLFALIRKILLESDFTMTTDTLRCKYCPYKDI
                     CNR"
     gene            complement(20130..20531)
                     /locus_tag="PKX59_03310"
     CDS             complement(20130..20531)
                     /locus_tag="PKX59_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOZ89927.1"
                     /translation="MKPFVVRVYGILINKNSEVLLTHEKEGGIEFTKFPGGGLEFGEG
                     LRECLVREFLEETKQRVEVMEHFYTTDFFIESAFNPGLQLISVYYKVSCINFILPEDA
                     NDIHFFWKPLKELNGEVATFPVDRLVEKLLM"
     gene            complement(20600..21466)
                     /locus_tag="PKX59_03315"
     CDS             complement(20600..21466)
                     /locus_tag="PKX59_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89928.1"
                     /translation="MKNILKFSVVFLFLAVYACKDYKPEVDRLNRERDSLINTSFLKD
                     STIDSFLGDFNGIEASLDSINQIHAAITLDRKRDPEMGGTVKDRIRKNMESIASLLDQ
                     NSQRIAELTRKLKSSGVKSAQLNKMITSLNLKIAERDSMIANLNNQLGDAKIEITGLR
                     TNIETLNSTIGEREKTIEEKVNQINTAYYAIGTYKQLRDKKILNKKGGFIGIGKTKTI
                     NPDIDNSAFTKVDIRQFASVELNTKSVKIMSSHPASAYTYEKDKKKYQSLKITNPDLF
                     WSNSKYLVIVTK"
     gene            complement(21592..22914)
                     /locus_tag="PKX59_03320"
     CDS             complement(21592..22914)
                     /locus_tag="PKX59_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOZ89929.1"
                     /translation="MKKLFLALVLLFFATTTVNAGIVYRFNFQSEVDRGMARIFSKAL
                     QEAHEQKADLFLIHLNTYGGMLDAADSIRIAILNSKIPVVVFIDPNAASAGALISIAC
                     NRIYMRSGSSIGAATVVTEQGEAAPDKYQSYMRGIMRATAEKRNRDPRIAEAMVDPRV
                     VIPGVNDSGRVLTFTAEEALTNKYCNAIVETEADILKLENLNSDKIIEFQPSWVDNII
                     SFLIHPALSSLLILIMLAGLYFEFQAPGTIFPIAASFVAAVLYFAPLYLEGLAANWEI
                     LLFVSGVILLALEFFVIPGFGVAGIVGIICTTMGFALSMLNNKGWDFTFTSSEQITQS
                     ILISLGAMLGAVFLIFYFIGKINDSKRLSKLTLQTTQPSEHGFRAHTDIDTIQAGMRG
                     TAFTALRPSGKIMVDGRVYDAVAETGWIERQEQVEIVSVGLTCTVRTC"
     gene            23060..24052
                     /locus_tag="PKX59_03325"
     CDS             23060..24052
                     /locus_tag="PKX59_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOZ89930.1"
                     /translation="MKIKVSLLIATAALSISTVIAQEGFRTIAGKKCQLYKVVSKDTW
                     SGLSRKYHKEIDELKAVNPGINDLKIGQIINIPIENTVNDKSENKSDTSAEIKMRKPI
                     AVINAPVKNNNKVHVIEKGETLFGIARKYDVSPNDIRKWNHLDNDGIKAGQKLIVSST
                     ESVAIKTEVKKQESKNETILQPLDDKKLEAKNNEVKNDSKLIAEASNKVIPPSPSPSE
                     MEYKPMRTEVKRSNGKSGAVIEVNESGMASWIRDGDINQSKFYALHRNAPPGTIIKVT
                     NRMNGDYVFVKVVGTLPDTGDNDKQILKMSEAAAKRIGAINEKFQVELNYGMTN"
     gene            24203..25381
                     /locus_tag="PKX59_03330"
     CDS             24203..25381
                     /locus_tag="PKX59_03330"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOZ89931.1"
                     /translation="MKEVYIVSAVRTPMGSFGGKLSSFSATRLGAIAIKGAIDRIKLN
                     PSEIKEVYMGSVLQANLGQAPARQAAKFAGLPDTVQCTTINKVCASGMKAVMLAAQSI
                     MLGDNDVIVAGGMESMSNVPFYMQNMRWGNKYGDVSAVDGLAKDGLTDVYHNYAMGNA
                     ADLCARECNFSRQDQDTYAIQSYKRSADAWAAGKFKDEIVPVEIPQRKGDPILFAEDE
                     EFKNVHFDKIPGLKPAFSKEGTVTAANASTMNDGAAALVLMSKEKAESLGLKPLGRIR
                     SYADAEQAPEWFTTTPSKALPRAVDKAGLKMTDLEFVELNEAFSVVALANMKEMNLDP
                     AKVNVNGGAVALGHPLGCSGARILVTLLNVLKQNNAKIGGAAICNGGGGASAMVIERM
                     "
     gene            complement(25471..26802)
                     /locus_tag="PKX59_03335"
     CDS             complement(25471..26802)
                     /locus_tag="PKX59_03335"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOZ89932.1"
                     /translation="MNKLYKYFAIGAAMLSIHGVALAQATKEVNVDGLKVILRHTPKE
                     VISVRLYVKGGTANYTKDKEGVESLAFSIAMNGGTKKLDKVAFNDAAEKIGTSFEASS
                     GLDYGNLSMTCIKPNWNESWNLFADAVMNPAFDSKEFDLEKEKALSGAKQRESDADGS
                     LSNIAMEETFKGRNYAKDPQGSVASLEKITLADVISHYKNVIGKKRCYLVVVGDVNEA
                     DLVGKIKATLGKMAVGTAPVVEPKLVITKGSEVIVDRDIATNYLMGIMSAPSLSTEKG
                     VQMDLAMQMLYDRFFVELRTKRSLSYAPAAGYMRNAVSSPYSYIYITTTDPKKAIDVM
                     VSIIDSIKKTGFTEKELKDLRQGYLTNYYQKLETSEAQSGALGRSEATGSWKLDESLS
                     DMVNNVKLKDLNNTFNEYTAAIKWSYLGKKDAISKDDFKQTKHDKVLQSPY"
     gene            complement(26814..28184)
                     /locus_tag="PKX59_03340"
     CDS             complement(26814..28184)
                     /locus_tag="PKX59_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOZ89933.1"
                     /translation="MLKKITAFATAFIVAGTAMAQKNLPSNFHMKKLGNGLEVLVIED
                     NSVPLVTIEICVKNGSFTEDSAFNGLSHLYEHMFFKANKAIPSQEKFLERVNELGISF
                     NGTTSEERVNYFFTLSNKLINEGLEFMNNAIRYPLFLEQEMKNENPVVDGEFQRAESN
                     PAFFLFQDFNKQMWGSNYVRKNAIGIHDVILSATPAKMNVIKDKYYYPNNSILVVAGD
                     VEHNAVFEKAEKMFGDWKPCDFDPFQKWPIPEFAPLVGQSKFLTVDKNAKMPIMMMGF
                     HGPDTRNDVKSTYAADVFSTILGLTSSEFQKTLVDGGYALQAGLGYQTCRYTGPIQLF
                     MVPNPMKMKSFYGKLMEQVNRFDAPDYFTDEQLETAKNKLIMDDIYNKEKTSAYVHTV
                     TFWWASASIDYYTTYNDMIKKVTRKDIADYVDKYIKGKPSVTGLLLNPKMKESLGINS
                     ADEILK"
     gene            complement(28266..29825)
                     /locus_tag="PKX59_03345"
     CDS             complement(28266..29825)
                     /locus_tag="PKX59_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89934.1"
                     /translation="MRKFLHTCLIAGLSFFTLQSMAQIQYPGIIDSTFGVNGKVTSSF
                     NSFACVARAIAVQSDGKIVVAGESNNGTFNRFAVARYLADGTIDNQFGTGGKITFSIG
                     TGNSIARSIAIQNDGKIIVAGNLENQTYSHFAMARLTTAGALDNQFGANGIVQTALDL
                     GFDIAYGVAIVPSSGKIVLGGVSFDTLANGEFAVARYNTNGTLDGGFGNSGKVRTPLG
                     TQWDQANALAIEPGTEKIVLAGRTLSTYALVKYNTNGTLDANFGLNGKAIYTIGSMSE
                     IINTIYINPDGMIVAGGTTQTGSNTDMVLMRFLSNGQIDNNFGVSGISLFDLGGTDII
                     FSIAPQGDYLVGAGSSYVGAAGDFVAMRFDTLGSFDLVFNNMIGYNITSFGNPSGANA
                     VAVQQPDSKIILAGSAVVGTVNNFAMARYTSDFTFVGLNEVNASHAKLQMMPNPASDF
                     IAIEASTPYNRIEIKDITGKKVEDVTPGSLIRSYSLDVKNYSSGVYTATVYFDSDIKT
                     QKFIVTNTTNN"
     gene            30044..31351
                     /locus_tag="PKX59_03350"
     CDS             30044..31351
                     /locus_tag="PKX59_03350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOZ89935.1"
                     /translation="MSQVELKMPKMGESVAEATITKWLKKEGDRVEQDEPILEIATDK
                     VDSEVPSTAAGILVKQLYNEGDVVAVGKSIAIIGDSASATVPTSSVPKAEEVKIAAAP
                     VIATQAATVATTVSASSKFYSPLVRNIAQQENVSSAELDAITGTGAEGRVTKKDILQF
                     IENRKSGNGQITQTTQAPTVQPATTPQAKAPAATPMTDGDVEIIEMDRMRKLIADHMV
                     MSKHTSPHVTSFVEADVTNMVLWRNKVKNDFEKREKEKITFTPLFIEAIVRAIKDYPM
                     INCSLDGTKILLKKNINIGMATALPSGNLIVPVIKNADRMNLVGLTKSVNDLSGRARK
                     NKLSPDEIAGGTFTLTNVGTFGNVMGTPIINQPQVAIMAAGAIRKKPAVIETEFGDTI
                     GIRHMMFLSLSYDHRIVDGSLGGSFLRKVADYLEQFDVNRNLL"
     gene            31358..32956
                     /locus_tag="PKX59_03355"
     CDS             31358..32956
                     /locus_tag="PKX59_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HOZ89936.1"
                     /translation="MRFVFAIIPILWLNIAIAQQSKKPVKKIVLGKDQVSEMKKAGSE
                     YFKSENYKTALPIYQQLQENDPNNAEFNYRLGLCYLNTNVNKKLAAGFLIKAAEGKDV
                     PKDVYYYTGRALLILEEIDDAVDAYEKFKIAHGGKVSPKLNLENNVEWCYHFRDMKKT
                     PIEVSFTNLGKLVNSPNNDYRPVCDMLGNVVYFSSNRKSNTGAIVDGYGEVISDGYFT
                     KKDTGYSKAKNLGPNINTMYYEEPLQLNSNSDKLLVFKEGGSISSPLYTSELNGKSFE
                     KAVAINKFPASKVDGACMTDDGKTIYFSADLKGGKGGKDIWMTTLNSKNEWSNPVNLG
                     DAVNTKYDEVNPWLFFDNQTLFFASEGHNSIGGFDIFFSTKPHESMDWSAAKNVGYPL
                     NTTDDNKYFSLTADCKTGFVSQTTEHGSGELDIVKFVCTNPIIKQEKVVCDFTFLKAD
                     GTFARDANCMIIRNDTGESSGVKTINGSNGKLYVYLGPGNYKVKVKGVKSGRADEDFI
                     ITGNEPKMQLVKTFKLTPPVKEGK"
     gene            32958..33728
                     /locus_tag="PKX59_03360"
     CDS             32958..33728
                     /locus_tag="PKX59_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOZ89937.1"
                     /translation="MNLKLHRPLAFFDLETTGVVIGMDRIVELGILKILPDGTKTNKT
                     WRINPEMHIPEVVSKIHGIYDKDVADCPTFKEVANDVNHYLNNVDLAGYNSNRFDVPL
                     LVDEFLRAGIEFDIRGRKLIDVQHIFHKMEQRTLSAAYKFYCNQELIDAHSAEADVRA
                     TYEVLEAQLERYSDQLQPDAAFLHAFCNNSKNVDLAGRIVYDDKKREVFNFGKYKGRL
                     VTDVFKTDPSYYDWMLKGDFSRDTKQVVTALRLREREN"
     gene            33805..36057
                     /locus_tag="PKX59_03365"
     CDS             33805..36057
                     /locus_tag="PKX59_03365"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOZ89938.1"
                     /translation="MGSDNNSDKNNLPSVSASGKGSKVVIDLEAEKKEISKRYRHLLR
                     ACKSDLDKKDKKLIRLAFDTALEAHKEMRRKSGEPYIYHPIAVAQIVAEEIGLRSAIP
                     VVCALLHDTVEDTYLTLEDIRDLFGPVAEKIIDGLTKIAGVFDHSSSLQAENFRKMLL
                     TLSVDVRVILIKLADRLHNMRTLGSMARDKQLKIASETAYLYAPLAHRLGLYNVKTEL
                     DDLALKYTEPEVYKTLAEKLRDSKKERDKFISEFIHPIKDELNKQGFKFEIKGRPKSI
                     HSIWEKMKKQGVTFEEIYDLFAIRIIIDSPQNQEKADCWKVYSIVTDSYRPNPDRLRD
                     WISTPKSNGYEALHTTVMSDQGKWVEVQIRSQRMNETAEKGFAAHWKYKESSQESALD
                     EWLTRIREMLENPDSNALDFVDDFKLNLFADEIFVFTPKGELKTLPVNSTALDFAYEI
                     HTDIGSRCIGAKINHRLVPISHKLNSGDQVEILTSDKQRPKEDWLSFVITAKAKGRIK
                     SSLKDEKRILGEQGKEIFDRKIKHLKIPVAEVNMNDILLYYKLPNVLELYYRIAMDAV
                     DLKQLREAVNYKDQRVRSQRTEPTLEELVTQARGSSDMLVIGENLNKIDYKLSPCCNP
                     IPGDDVFGFITINDGIKIHRVNCPNAMQLMSNYAYRIVKAKWTGQEQLSFLAGIKITG
                     MDDVGVVNNITKVISSELKVNMRSISIESNEGLFEGTIMLFVHDTEHLTKLMKKLSAL
                     SGILNVTRIT"
     gene            36060..36560
                     /locus_tag="PKX59_03370"
     CDS             36060..36560
                     /locus_tag="PKX59_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89939.1"
                     /translation="MYVSFDQLPDDSRIWIFQSDRLLSEEEIESINPILNFFISDWTA
                     HQQTLRASFTFMHNHFLIIGVDENMAIASGCSLDKVFHVVRACGQKINTDFLNRLHIA
                     AMIQNVITLHSIKQILDIAENRNNNEPLMIFSNIIETKSEMKNNWLIPIEKSWLAPKL
                     KSGSKI"
     gene            36577..36927
                     /locus_tag="PKX59_03375"
     CDS             36577..36927
                     /locus_tag="PKX59_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOZ89940.1"
                     /translation="MNFIIKLIISTLAVMMVSYILPGVHVQSFGIALMVACVLAFLNA
                     LIKPILVLFTLPITLITLGLFLLVINAIIIIITDRLIDGFEVSSFGIALLFSLILSIV
                     TSVMESLAGQNQRQ"
     gene            complement(36976..37782)
                     /locus_tag="PKX59_03380"
     CDS             complement(36976..37782)
                     /locus_tag="PKX59_03380"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ89941.1"
                     /translation="MLKSISLVAVLAVFASGAFALNPSKEYKVLPDKYGMTYKEEKIK
                     TPDGAILNAWMFELPKKSTNWIIMSGSGDGNMADNIEIANSFLSAGYNVAMYDYRGYG
                     SSSDFKIDPDLFIYPQFIVDLNAVCDYLRKSRAITKFNLYGKAIGAGLSIGVGANRTE
                     TQKVIADGPWIGLEVMKKKIKDKTGKEVVMPFGFDKNHEPMYAMEKPKGIKGLMVIVS
                     SQDNLINPTDVKQIKGVSETYVVKNSPSNAENFTIDKNVYFEKISKFLGN"
     gene            complement(37937..38758)
                     /locus_tag="PKX59_03385"
     CDS             complement(37937..38758)
                     /locus_tag="PKX59_03385"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOZ89942.1"
                     /translation="MSQDTSFTVYGHRGCRGLMPENTIPAFLKAIELNVLHLELDVVV
                     SADSQVVVSHEPWINNENCLMPDSTPLPDFKRAAYSIYKMPYEQVKGYDCGSKFYAKF
                     PGQHKMVVGKPLLSEVINTADTFCCNTKGVLPYYSIEIKSKKSWIGIFQPETKQLVDL
                     VMKVVQQYGEKERFVLQSFDPEVLRILHDDYPEYTLAALNETNRDVAAFFNELGFVTT
                     IYSPWYVLATAKAIAYCKQQEIKIIPWTVNDDKVFLKLKYSGVNGIITDYPDRYR"
     gene            39019..44328
                     /locus_tag="PKX59_03390"
     CDS             39019..44328
                     /locus_tag="PKX59_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HOZ89943.1"
                     /translation="MKKILGLDLGTNSIGWALIEQNFDKKEGSVIGMGSRIIPMSQDI
                     KDEFGKGNSVSQTADRTKFRSVRRLRERHLLRRERLHRVLNVLGFLPEHYANDIDFEK
                     HFGKFKDETETKLVWKKNGKKPNGKDKFEFAFQSTFEEMLNEFKKSQPDLLNKKNKKG
                     EEFKIPYDWTIYYLRKKALTQKLAKEELAWLILNFNQKRGYYQLRGEEEEENQNKLVE
                     FHSLKVVEVKAEIKYILTLENNETYYRLSETPLLDWKDTNRDFVVTTYLETDGTVKKD
                     KDGKEKRSLFLKDDLVFIKKKTTKITKSTKNKNETVEEKSIKIIDVKEEKQNEIWYSL
                     ILENGWIYRRQSKVSLEDWKGKVREFIVTTDLNDDSSIKTDDEGKESRSFRAPKEDDW
                     TLIKKKTEDDLSKYIGEYKTKNGIDGTVGTYIYDNLLRKPNQKIKGKLVRTIERKFYK
                     YELREILKKQIVLQPELFSDDLYNDCVRELYKNNEAHQLQLNKRDFIHLFLDDIIFYQ
                     RPLRSQKSSIGNCSLEFRLHKINKKDDKGNSIKNEFEKDENGKDIEVKEYLKVIPKSN
                     PYYQEFRLWQWLYNLKIYKKEDDTNITADFITSNDDKITLFDFLNSRKEIEQKPLIEF
                     LVKQRELKNPANNNLNKKVQTALSKKIKEETEKYRWNYVEDKKYPCNGTGTQIRTRLE
                     RVTNVSVSFLTREIEQHLWHIIYSVTDKNEFEKALKSFSKRHQIDETSFVENFKNFPP
                     FISEYGSLSEKAIKKLLPLLRIGKYWNENAINDKIKKRANSISERLKSISKKEDIENI
                     SDDDIQKQILKSFYGLTDLTQGLQLYQASYLIYGRHSEASDLQSWNSVADLEKYLKEF
                     KQHSLRNPIVEQVVTETLRVVKDIWTHYGKGVENFFDEIHVELGREMKNTAEDRAVLT
                     KQVTSNENTNMRIKLLLAELKNDTTIEGVREYSPSQQEILKIYEDGVLNSNVEIPNDI
                     SEILKKFKESDIKKQPTKTEVNRYKLWLEQKYRSPYTGQMIPLSKLFTEAYQIEHIIP
                     QSRYFDDSFNNKVICEAAVNQLKDKQLGLEFIKNHSGQIIDLGQNQSVTLFTEQQYTD
                     FVKEHYDKNRSKRTKLLLDEIPDKMIERQLNDTRYISKFISSVLSNIVRANKDDDGVN
                     SKNLLPGNGKITSELRQDWGLNDVWNDLILPRFERMNELSQSNHFTATNKEGHTIPAI
                     PLELSKGFQKKRIDHRHHAMDALVIACATRDHVNLLNNQSAKSDNSRYDLQRKLRKVE
                     KWTDKEGKERDKFTDFYKPWSTFTQDTKNELEKIVVSFKQNLRVINKASNHYEKIING
                     KKVLVKQEGSNWAIRKPMHKDTVSGLVQLRKTKTVSLSVALENYNDIVDKTLRKHILS
                     LINQNYDKKKLTKYFKDNENKWNNRDISKLDIYYLENDNVASRVNLDISFNKERIEDT
                     ITDTGIQKILLKHLINYEGQTDEKGKAIAPETLAFTPEGIDEMNKNIITLNNGKSHQP
                     ILKVRTYEPKGNKFTIGETGNKKDKFVEAAKGTNLFFAIYEDENGNRSYETIPLNIVI
                     ERQKQGLCSVPEQNEKGHALHFHLSPNDLVYVPQVDENLDAINFGELTIEQSNRIYKF
                     TDGSGIMANFIPSKIAEVIFNINKLKQESLKINYPIQNEIGVGSQGSKNERAISGEQI
                     KSICIKLNIDRLGNITPSKKTNQKSTSSFVNEEHSIYYNKVTFNNLQQSDEKMFMHTA
                     NMNPEERMAYLQKLRETTQENISEAEKRAILNQIKINPPDENT"
     gene            44393..>45235
                     /gene="cas1"
                     /locus_tag="PKX59_03395"
     CDS             44393..>45235
                     /gene="cas1"
                     /locus_tag="PKX59_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HOZ89944.1"
                     /translation="MIKRTLYFGNPAYLSLKNSQLMVRLPEVEKNQQLDVAFKQQAHA
                     TIPVEDIGIVLLDHQQITITHGLLDALMENNVAVITCNNTRHPAGLLLPLQGHTLQHE
                     RYSDQLSASEPLRKQLWQQTIIQKIKNQAAGLQLQNIDSTRLYPIYNNVKSGDSENAE
                     GVAAAFYWSNIFTKIPATIEHAPGNLFKRQRDGLPPNNYLNYGYAILRATMARSIVAA
                     GLLPTKGIHHHNRYNAYCLADDLMEPFRPFVDRIVIDIILKHGIIDDLTKAIKADLLT
                     IPVID"
BASE COUNT        15871 a         7882 c         7982 g        13500 t
ORIGIN      
        1 ggatttgtat ctaattgatt tgtagtgtgg aaatttgacg ttagtcagaa gcataaagac
       61 aaacgacaca gatgaaaaga ccatttaaca tattcccgag tataggatat agaaatagcc
      121 aatttcaaat tgtttcatcg gttgacaatt tgaaaattga catttacgac caagacaaag
      181 tagttaagtc ttttgaagct aattctaaaa atcccacttt actgacaagt cttggtacaa
      241 caggcaaatt gattgcaaaa tgcaatttta acaatatcgt ttttcagcaa gaattggaaa
      301 taaaagaagc ctttaggctt ggttcaagtg aatttaaaaa ggcttttgtt tttgacgaca
      361 cagaatattc cttctttctg atgaaggaca gattattgct ttatgatgag aaaaagaaaa
      421 tattactgac tgagaaccat tatagtccaa cagaaattta taaaatcaac ggaacgaatt
      481 ttctatttgt aaccagagta ggaaactctg caagcgggat tattaacctt ggagtttaca
      541 acacggaaaa tttctcaatg gtcggagaat tattaaatga ttatcgtgaa attaagatac
      601 tagccgacaa caacaaagct tggctttata acataaaatc caaaacaatt cattgttacg
      661 aattagcaca ccaaaccaat aaatacttaa ccgagttaaa atcatttgaa aattttaatg
      721 attacttttt agatgatgcc aacctaaata tttatatcaa tcataatgaa gtattgaaaa
      781 tttcaagctt gaacaatctt cataagtact tcgaaatacc aaaaaatgaa aataacgcaa
      841 tagacaaatt aggtaatgta ttcacacttg aaaacaagac ccttaaatgt acaagtttac
      901 atacaactta ctctgaaacg gtcaatcctg attttgaagt taatttggaa accgataatt
      961 tcattcatat aggcaagcat ctaaagagtg aaggcgaatt aaccgaccta attagaaaag
     1021 cagaagaaat tagagaagaa gtgatttctt cacttccaga tgctaaaaca tatcattatc
     1081 acgcactttc tgaatcccaa aaaatatcag aaatatatac aacccacaaa atttatccga
     1141 ctaaaaatgg cgtttttatt attcaaaaga gaataaaaag agattttaat ggagtcactc
     1201 taagaaagtg ccaagccacc tggactgcta caccatacaa aatagaaaat acggaaataa
     1261 cccttctgct tttcaattcg gagaaagtag atattttaat cgacaaagct cctaacctga
     1321 cagtttacga atatctaaaa gatatgcttg tcgtaagtca tcaaagcact agaattctat
     1381 tttcaggaag gaataaagtt acgctcaaca atgaagattc gatagaactc ttcaccacaa
     1441 acgaaatagc ctacttttta attaaatcca aagaaaggta ttcactttac cgttcgacca
     1501 atccaaataa ttccatttta gagcaaatag aaattctaaa tcctgaccta ttcaaaaaac
     1561 atcaaatcat atggtatcgt ggtaaagaaa aaaatatatc caacacaaaa tatttgaatg
     1621 ccttcgattt gaaaaattgc acaagaatac ttatcgatga gcaaaaggtg caacactcac
     1681 tttttaaaga cgctttggac tttaagtttt ttgagaagta tgctttatcg tctaatcaaa
     1741 tagtttttaa cccagcaact cttgaaataa aagatgcatt tattggcaac attgaatctc
     1801 attctaagca actgaacaaa attgtatcgc atcgaactaa caaaatctac ctttccaagt
     1861 ttaatcctca aattgcaaag tatgaggtat gcgaaatatc aattgatgat aacaaataca
     1921 gggaatcata tctttcccca aacggtcaat ttttggttct tcaagatgaa agcaataaat
     1981 attcctttta tgatatcgaa aaaggtgaaa ctataaactt catttccggc aactttttag
     2041 cgtttagaaa tgacggtagt cttatagtag agcaagatgg tacaaaagca gtaaaaataa
     2101 ttgacccaaa aacatttcaa gacattacgc ccgctaacta tcatcattat agatttatga
     2161 gtccagatgg aaaactttat gcacaagtgt catccaaagt aaggtatttc cataaactaa
     2221 atggtaaaga aataaaggca gttgaagttg ctagaattag acaagactta gacgagccga
     2281 gtattttcct taaagaagat gaaagagaaa aagcaaagat taaggtgtcg gagaatagaa
     2341 aagattttta cgattcattt aaaaatcaat ttgaagaaat agggattgaa gattacaaca
     2401 aaatcacatc acaatcagtg gttaaagtag ataaattcac tgaaattggc ataacaggca
     2461 caaatattat tacagaagtt cactttccag acgatttagc tttctacaat tattcagctt
     2521 tcagttacga caacaactat tttggatatg ttggcaagcc atcgtcaaat ggactaatcc
     2581 atttatttaa aattgatttt gacgaagtaa attcaaaact tttggtgtct gacacatatt
     2641 tgagcagata tccacgttac gcatcttggg tctgtggatt ttccaaaaca ggttatttcg
     2701 caacttatga tagcacacca aacacataca tcctttttgt tgatgatagc ctttttgaca
     2761 gcaaaacaac agaagttgag ttgagacaaa atatttataa atcaaaaaca aacatttatc
     2821 acacttacaa taagtggaat gaaataaagg gaaaaaattt tctttgtttc agtccgacag
     2881 gtgattattt agcattatct gaacaaggtt atgaaccttt gactttaggg gggtatggac
     2941 atcaagaatc taatgttgtt catattgcta aaacagaatc aggtgctatc atagattcat
     3001 ttacaggaca tggcgataag ataaaggatg acactcgcaa aaaagtaacg tttgtagcat
     3061 tttccgatga cgaaagtaga attatgacac ttagctctga cggtgtagta attataaggg
     3121 acatcaacat aagtgattat gaacagaatg acagaatgcc agctgccaac aagcgtttgg
     3181 cggcaatggc gggtgacgtg gttaattgaa cattctacct cgcatcagct tttgtggtgt
     3241 attgacagtt ttgtgctccg aaatccgcca ctgcgccaag cgccaaaccg ttatagccaa
     3301 tggtaggacg accgttctaa ccaagacagc cgacctgaat ttagccttca aaacattagc
     3361 aaaccataat ttaagccgac actcattgcc gacccttctg tattttttat tttactcaca
     3421 agttgtttgt tttttaaagc tgttcgtgaa tgcgaaaagc atttccccac cgcacttttt
     3481 ttaattcaat tttattgcct ccgcacaagt ggcacatttg gttttgcccc gacactcaaa
     3541 gccaacgctt cgcaaaacca aaagagccac tttttttcct acgcacagaa agaagaaaca
     3601 acaaatcaaa actttttttg aaatttattt gtcgattaaa aaagaatgtt tacttttgtg
     3661 agtgaatact aactaacatt aaaaagaaag tatgcaacaa tttacagaca gacaaataga
     3721 aataatggaa gcggctacca accgtatttc aaaatatggt attcagaatt tgacaatcaa
     3781 aactttggcg gaagatatag gtttatcaga accagcattg taccgacatt ttaaaagtaa
     3841 aaatgaaata ctattgagcc tattagagta ttttaaaaca gaaatgaaaa ctcgtattca
     3901 gtctatttct tttaaaccta ctgacactac agctgacgaa ttaagagcaa tttttaattc
     3961 tcaattacaa acttttacaa acaaacctgc aattgtgagt gtcatctttg cagaaagcat
     4021 tttccatttt gatgaaagtt tgagcaataa agtagctgaa attatggata tgatgcagga
     4081 ttatgtgaag acaaacatta caaaagggca ggaaaaagga caatacaata aactaattgg
     4141 aagttctaca cttacaacca tcgtaatagg cggaatgaga atgacagtat taaaatggaa
     4201 gctatcagga cataaatcaa accttgttaa agacggacaa actgtcttag acgggatttt
     4261 aaaaatgatt gaaaaataga tatatgaaca cacaaaagaa atttacccca tttcacagac
     4321 tattgcactg gataatggca attgcgatgc ccattttatt cattacaggt ttcttgagaa
     4381 tgtattggat gaataaaaat aacatcgttg ctgttattga aagcaaaaca tcggcaattc
     4441 caaaagaaca aatgacagac attgccaagg caattagaga acctatgtgg caatggcacg
     4501 aactttttgc tcacgtaatg atttttgctt ttttagctcg aattatttat atgcttgtaa
     4561 aaggcattcg tttccccaac cctttcaaga gtaaacaacc actgaaagaa cgcttacagg
     4621 gttttactta tatttattta tatctttttg ttttcatttc agcatttact ggtatttgca
     4681 tagaaaaaga tttgctttct acttatcacg agcaaattga agccgtccat aaatggggaa
     4741 tttattggtt tcctattttc attttactgc atttagtagg aattgtatta gctgaattgt
     4801 caaacaaaaa aggcattact tctaaaatga ttggaggaga ttaaaccaaa ttaaatataa
     4861 acaagaataa aaaaatttac cttaatatgt tagtgaatac tcgcaaactt attttctttt
     4921 cgatagtgat tatcggatgg aatacggttc gagctcaaga agtttggaca ctgaaacaat
     4981 gtattgatac agcaaaagtg cataacaaaa cattgcaaat taatcgcaac aacatttcca
     5041 ttagtgagca gagaataaaa gaagcacaag ctaaccttat tccaaaaatc acggcaaata
     5101 ccgattataa atattttacg gaattgccta cgcaattaat gccgatgaat gcactcaatc
     5161 cgcaagcacc cgaaggacag tttagagatt tacagtttgg cgttccgcat aatatcaacg
     5221 caaatattca attggcaatg ccactttata atccacaggt ttacggagca attcaaaata
     5281 caaaaattgc aaatgaactc actcaattgc aatatcaaaa atcggaagaa caggttttgt
     5341 atgatattac aactttgtat tacaatgctc aaatattgaa gcaccaattg gagtttttag
     5401 aaactaattt agagaacaca aacaaactat taaaaaatat acaattgcta aaagaacaac
     5461 ttttggcaaa gggtacagat gtaaataaag tgcaattaca agccgaacaa ttagctactc
     5521 aaaaagaaaa tgtgaataac aaatacattt ctattctgaa tgccctgaaa ctcaatatgg
     5581 gcattgcttt agaaaccaaa attagcgttg aaagcgctat ttcttttcaa acaacattgg
     5641 aatacacttc aaagacaaca ttggatattc aactcattca aacgcaaaat agatttttaa
     5701 atgctgaatt aagtacgctg aataaatctc gctttttgcc ttccttaaac ttgattgctt
     5761 cctacggaac aactggtttc ggttatgata aaacacctaa cgatttcttg aaattctacc
     5821 ctatcagctt tgcaggagta cagctttctt atcctctttt caatggagcg gttacgctac
     5881 gaaaaatcaa ccaaaagaaa ttagaaatca acaataatga attgcaagtt caactgatga
     5941 acgataaaaa taaaatggaa attgaaaata gcacaagaca aaagaatgta gctcaacaaa
     6001 cggtcttgaa caccgaaaag caaatcgctt tagctcaaac catttatgat caaactatca
     6061 ttcagcaaaa acaaggaaca gcaagcctaa ccgatgtttt attagcagac aacgcattgc
     6121 gtgaagcaca gcaaaattat ttatcagcaa taatagatta tttgaaagct gatttagaac
     6181 ttaaaaaagt aacggggtca attggcaatt aagaattaaa aattaagaat taaaaattaa
     6241 gaattaaaaa ttaagaatta aaaatcaagg atataatgaa aaaaataatt tggataatag
     6301 caggtgttgc tgtggttggt ttgatgatgt tcaaactttt aagcaacaaa aaaacaactg
     6361 aaaatagagt atatcaatac gataaagaaa aacctattac cgtaagtgtt gataccattc
     6421 gactacaaaa cattgttgat gcaggcaact acacagggac ttttgaacca aataaggaaa
     6481 caaaaatcag tgcggatatt cagggaaaaa tcaacgcggt attggtagat gttggcgata
     6541 tagttcaaaa agggcaaacg cttatccaat tggataactc attgttaaaa ttgcaattac
     6601 aaactgtaga agtgcaaatt gaaggattgg aagatgatgt aaaaagatat acaattttaa
     6661 ctgaagctga cgcagtacaa ggcgtgcaat tggaaaaatc aaaattaggt ttgaaatctg
     6721 caaaaattca acgagcaacg ttgttagagc aaattagcaa aacaagtatt aaagcacctt
     6781 tcaacggcat tgtaacggct aaactcaacg aagaaggcgg ttttgctgca ccggggattc
     6841 ctttgctaca aattacagac atcaatacct tgcgttttac ggtaaatgtt cctgaaaaag
     6901 atttggtaca gttccaaact aacaaaactt acaacattac ttcggatgtt tatcctgaaa
     6961 ttccgctttt tggaaaaata ataatgacag gaagtaaagc gaatatgggc aatagttttc
     7021 cggttcaatt tcaagttgaa aataccaaac aattatctat aaaatcagga atgtttggaa
     7081 aagtaaacct ttcttctact tcgcaagaac aaggcatact cattccgtca tctgccatta
     7141 ttgaagtaga tggagtggca aaagtttata tcgtaaaaaa tggaaaagcg gttttacaaa
     7201 ctataacaac ctctaagaat atcggaaaca aaacgcttat ttcaagtggt ttaaaagaga
     7261 acgacatcat tgttacgaat gggtttatta atctgtttga aaatgcgaat gttttaatta
     7321 aaaattaaaa attacgaatt acgaattacg aattaaaaat caagaatatg aaagagaata
     7381 ttgtacaaca aaagagtttt gcttttgcaa ttcgtattgt tgaattgtat aaatacttgc
     7441 aatcagaaaa gaaagaattt gtcttgtcaa aacaagttct acgctcgggt acgagcatag
     7501 gagcaaacat tgaagaatct attggaggag cttctgaaaa ggatttctta cataagttaa
     7561 ccatctctta caaagaagca agagaaacaa tttactggtt gaaattgcta catgctactc
     7621 aatatatttc cgaaaaggaa ttcaactcgc ttcacaatga tgcagaagaa atatgtaaaa
     7681 tattagcgaa aattcaaatc actttgaaat ctcgtaattc gtaattatta attcgtaatt
     7741 gaacaaaatg aacattacag aaatatcaat taaacgccca tcgctgatta tcgtactttt
     7801 cagcgtattt gctctattgg gaattatcgg gtttaaaaat ttgagttatg aattaatgcc
     7861 cgattttaac caaccagtag tggtcataaa aacagtttat cccggtgccg aaccgaacga
     7921 agttgaaacc tctgtttctc gaaaaattga agatgcactt tctaacttgg agggtgtaga
     7981 ttatttggta acgaaatctt taccgaatgc ttccatcatt attgccaatc tgaaatatgg
     8041 aacagatttg gacaaaacaa tgcaggatgc acaacgctac attgacaata tcaaaaagga
     8101 tttaccaaaa gacattcaaa atcctgtaat gagtaaggtt tcacctaacg atttgcctat
     8161 tatgtcggta agtgcaacca gcgatttaaa cccgactgaa ttttatcaaa aaatgaagga
     8221 cgattatctt cctcaaattc agaaattaaa aggagtagcg gaaattactg tcttgggtgg
     8281 agaagaacga gaaattcagg taaaggtgga caaagataaa ttgaaactct ataaaatttc
     8341 tttgtatcaa gtagtcgaag ccattaaccg ctcgggaatt gatttgcctg ccgggaaagt
     8401 tcagaccgac caagaaaata gttcagtacg tttggtaggt aaattcaatg accttagtag
     8461 tattcaaaat gtgcaagttg caatgccaat gcccggtagt cctgtttatg tgaaagacat
     8521 tgcagaagtg gtggacggca ttaaagagac aagttctatt agtagataca acggtaaaaa
     8581 tggaattggg cttttgataa aaaaacaaag tgatggtaat gccgttgatg tttctaaatt
     8641 aatcaaagag cagtttcaaa ctattgaaaa gcacaatgct tccaataatg tgaaatttgt
     8701 aattacagat gacagtacag ataatactat tgcagcagta aattccgttg ttttcgattt
     8761 aattcttgca gttattttag tttctattgt aatgcttttg tttttaagaa gctatcgaaa
     8821 ttcattgatt gttgctgttg ccattcctac ttcgctcatt accgcttttg gaacaatgtg
     8881 gttgttgggt tatacgctta atttgatgac acttcttgcg atgtctttgg taattggtgt
     8941 tttggtagat gatgccacag tggttttaga gaatattcaa cgccatttgg atatgggaaa
     9001 agacaaacga aaagccgcaa tggatgggcg tatggaaatt ggtttttctg ctttgtcaat
     9061 cactttggta gatgtggtgg tttttgttcc tatcttattt ctacaggttt ttgtggcaga
     9121 tatgttgaag cagttttcgg ttgtaattat tgtaacggta ttgacaagtt tgttagtcgg
     9181 gtttacactt acaccttggt tagcttcacg gattggagaa aaagaaaatc ttcaacctag
     9241 caatttcttt aaccgctttt tgctttggtt tgaacatcag ttagataaat tcatcgcttg
     9301 gtatggcggt aaattagatt gggttttaag acataaactt ctatttacag gctttgtgat
     9361 ttttcttttt attggaacag cagtaatgat gaaacaagga attatcggta aagaactcat
     9421 tgcaactggc gaccaaggca aattcctctt aggattagaa tttgataaaa atacttccat
     9481 tcaacaaaat aatttgattt cagaaaagat tgaaacctac atattgcaac aacccgaagt
     9541 agcaactttg tttagtaatg ttggcggacc aagtacaggt attggaagtt tgggtgttgg
     9601 ctctgcaaat aaatcggagt ttactattca attaaaacca aaaaaggaaa ctaataatct
     9661 ttctactgaa aaatttatga ctgatttgcg agaggattta caaacaaaat tctctggtat
     9721 taattattcc attagtggat taggtttaat tccaaaaaca gcacccatcg aaattacatt
     9781 gagtggttca gagttaaatc gggtaatgca aacggcacaa aatttaaaaa tggttattga
     9841 aaaaataccc ggagcagata atgttcgttt atctgttgag gcaggaagtc cggagctaaa
     9901 agtaattcca gatagagata aaatgcaacg tttaggactg aacactgctt acgttggaat
     9961 gaatttgcgt actgcttttt ctggaaatga cgatgcttcg ttaactgaaa atggaacaga
    10021 atatcctgtg cgaatttggt tagataagtt cagtcgtcaa aattatgatg atatcaataa
    10081 cttgaatatt gtcaatccta tgggaatacc cattgaagta tcgcaatttg cttctatcga
    10141 aaaagacaat tcaccctctt tattagaacg aaaagaccga cagccagccg ttaccttaac
    10201 tgccgatgct ttggggcgtc catcgggaac agttgccgat gaagtggtag cttatgtgaa
    10261 aaataatcct ttaccaaacg gtattgaaat gacttgggga agcgacatta aaaggcaaaa
    10321 cgacagtttc ggtgcattgg gttctgtgct gattatttca ttcattttga tttacctcat
    10381 tatggttgct ttatatgata gttacatcta tccatttgta gctttatttg ctattccggt
    10441 tgcagccatt ggagcttttt tagccttgaa cttatcgtta agtcatttaa gtttatttgc
    10501 tttattaggt ttgattatgt taatgggatt agtaaccaaa aactcaattt taattgttga
    10561 ttttactaat caattaaaag ccgaagggaa accctatcgt caagccatta tcgaagcaag
    10621 caaaggcaga atgcggccaa ttttaatgac aactttatca atggcaattg gaatgttacc
    10681 cattgcatta gccacaggaa catcagcaga atggaaaaat ggcttggctt gggtaattat
    10741 tggaggctta ctttcttcat taatattgac tgtattctta gtgccaatgg tgtattacgg
    10801 agtggataga gtgaaggaga aattaaaaat taagaattaa gaatttaaaa ttacgaacta
    10861 cgaattaaaa gttatgaatt aagaatatga gagtagtatt ttttgttgtt atgtcagtgt
    10921 ttgttttaag tgtttttgct caggctaaag cagaagaata tagtttgaca atagaagttg
    10981 aaagtttacg taattcaaaa ggcattttac aagtagcttt atataacaaa gacggctcaa
    11041 taccagatga gtattacaaa aattatttta aaatagaaac tgcccaaatt acaaatggta
    11101 aaagtaaaat catatttaaa aacctaccaa aagggaaata tgcggtaaac attttacacg
    11161 atgaaaacaa taacggcaag attgacaaag gtttaatttt cccaaaagaa ggaattggct
    11221 tttctaatta ttcatctatc ggtttaacca accgcccaaa ttttaaaaaa gcaagttttg
    11281 atttatttca agacaaagaa attaaagtaa aagtgattta tatgtaacca atatggagaa
    11341 ggtagactct cttcaaaaac caaaaagaga ttgctttatt caaacaatct cttttatctc
    11401 cgtactttca aagtctcccg catttcaggg gactttgaaa taataaaaaa acattgaaga
    11461 gttaatacgt tttttatgaa aatagaacac agctaatttc acctcaggtc gcacagcccc
    11521 tgacagtgtg atttaataca agcaattaat tttaatacag cctaatttgc tgctattgcc
    11581 tccttaaatc atttttctat ttacttttga actaatgaat agatttaatt ccgatagtta
    11641 tcggaaagac tgaaattaca agcaaccctg aaaatgaaca cagatgcaat acaataaata
    11701 aaaatgaaac gacttttact tattctcgga cttttcattt catcaagtgg aatggcacaa
    11761 ctaacgggcc ttaccttgac aggaaatccc gaaagtttaa atggcgctac ttggacttat
    11821 cagcagacta ttaatggtac gtcttacgac cttaccggga ttttgtttaa gcctgtgggc
    11881 tctggtgttt ttcctgctgt tatcattaat catggtacgg gggggaatgt gaacggatac
    11941 agtaaaaatg tagccaagaa aatggttcaa tggaattaca cttgcatagc tgttaattta
    12001 tgtcattctg ccggtgcacc tatcggttca cctggcgatt caagcttgat aaattttggt
    12061 gccagcacca ataactatct ccgtgacatg aaatgctggg atattctggc atcattaaat
    12121 tatgtggata cgaattgtat tatggcattt ggacatagca gaggtgcata ccttacaaca
    12181 ggtttagttg ccacataccc tgataaattc agagcggctg gacatacagc aggaggcgtt
    12241 tctccccaat tggggttttc tgcacccact acaatgcttg cagctcagat aagctgcccg
    12301 tatattatac atcatggcga tgcagataac acagttccaa tagagatgga tagtttgctc
    12361 aacagcgtat ttaattcgac aggaattccg catcaatttt atcaatacag tggatatacc
    12421 cacagttcaa tcagtatgga tagtctaatg tttgccagaa cccaacaatg gtttgcattg
    12481 cacggttgtg caaacacaac atcaattaat caacagatat cttctgaaat gatttccata
    12541 ttccctaatc cgacatctga ttttatccaa ttcaaaactg aaaaaagaat tgatgtaagt
    12601 ttgtttgatg tgagcggaaa attattgtta tctaaaatag ttatgcccaa tgaacaaata
    12661 aatgtttcac aattgaacaa tggtatttat tttattacaa tcaatctata tcgaaaaatg
    12721 ttccatcata aaattatcat tcaaaaatga ggttattttt aaccctgata agtttgtttg
    12781 ttgttttaga tacacaagct caatttattg gccataatgg cgtgtatttt ttaggtacag
    12841 ccaatatgcc gattagcaca acaagtttta atgaccccaa tattagcggt gttgtagtgc
    12901 gttttagatg gaatgatttg gagcctagtc cgggcaattt caattgggca ttcattgatg
    12961 gagaaatcgc aaaagcagtt acttacaata aaaaaatatc tcttcaaccc ttaggaatac
    13021 ccaactggct tggcagtatc ggagcacagc agtattatac tattcaagac aatccatcga
    13081 gtcctaattt tggacaaata acaacaggag ttattccatg ggacagtgtt tatgtaagca
    13141 gattcaaaat ctttttgcaa aaccttgcta taaaatatgc gggcaatcct gtggtaacgt
    13201 atgtgaatgt gatcggtgtt aatttttcta gaaacttacc tgacacagtt ctaaccgata
    13261 ccgtaaccat aactaaacaa gcattttggt ctgtatataa ttataatgct gacagcttag
    13321 gtgcattaat aaatgaaatg actgactact atatgagtgt gttcccttcc accccactat
    13381 ggtgttctgt tgattatgtt acttttcagc ctaatgcaag tggacaagcc agaaattatt
    13441 tggcttcaat atattgcaat tatggtatca taaactaccc ggacaggttt ggtttattta
    13501 gagaagatgt ctcaggctgc aaccccaatt tgtccaatat agcaaataca tctcattggt
    13561 atctcatgca acaaaatcac tgtagaaccg gagcacaaat gctttggagt gttcaggatg
    13621 ggccatcacg aatgaatcaa tgtggaatat taccaaatac taaagcgatg gttttagatt
    13681 ctgcggtaaa taacggatta gcattaggaa tgcgctattt ggaaatttat ggaagtgaca
    13741 taacagatac ttcacttgct tcaactatcc aacaagcaaa caatgacctt attgcaaaag
    13801 ggttattgtg taattcttct acgggtttaa atgagcctat ttctgaaaat acattttcta
    13861 tttacccgaa tccagcaaga gaatacttaa caattaattt tgacccgaat caaaaaactc
    13921 aaatacaaat ttttaattca atgggaatgt tgttcaaaga attttctgta acaacatcta
    13981 ctcaattaga tatttctgat ttgccaaacg gcttattttt tgtacgactc aaaaactatt
    14041 ctcaaccgat aaaaaagttt atgaaacagt aatatgaaaa ccctacagaa ccttgactag
    14101 aattgagcag ctggtaacac gggttcctat gtaaagccct ccactcagga aactagctaa
    14161 attttctttt gaagaggaaa ttatttttga attttgcctt ccttttaccc cctcaaaatc
    14221 tacacaactt tatatctggt cactctgaag aaattccaac attccaagct ttccaacatt
    14281 ccaagctttc caacattgca actctcaaaa ctcctaccac attttcaaat tatctaatta
    14341 tctaatttcc ttacaatcta atatcacttc accttcaata gttttctcgt actcacattt
    14401 tcacctgata caacagtcag aaaataaatt ccgggattta atttctctgt attgattgtt
    14461 gtttgagagt tggtttttgt tttgtaaata gtattgccca atacatcagt caaaattagt
    14521 tgtgtttgat taatgtctcc tgaaatattt atctgactaa tgaaaggatt aggatatatc
    14581 atcagattgt ctgcactaat actattcaat gaggttgttg gaaatgcata tttagcaaag
    14641 taaccatcgc gcgagccggc acttaccaat gtgtttatag ccggtgcagg attaaagtca
    14701 acaacctgct caaagccacc gcataaaaac acttcgtcaa tgccgttaca ggtcatgttg
    14761 cggcaaaact caaaggcagg actgccacaa ccaaaagctg aaagataatt tcccatagag
    14821 tcataatgtg caagatacat atcagcaaag ccattactat ttagtgatgc aacagtaaca
    14881 tcagggtcaa agtcggcagt attaaaaaaa taaccactca ccaaaattga gctatttgaa
    14941 agttcggcaa tggtataaca aatatcagca ccattgccac ctattctttt agcaaaatca
    15001 taactgccac cattcgtata ttttgcaaaa tatccgtcaa agctaccttg tgccatcaat
    15061 gccattgtat cagcatcagg atcaaaatct gcaatgcctg aaaactgtcc acacgtcaga
    15121 atgtctccgt tatgatcaac cttcaatcca aatggataag cattctctat tggaaaagac
    15181 caatagtagt ttcccatgtt actaaatgaa gcagtgaatg ctgctagtcc gtcagcaaag
    15241 agtgtatcgg gactcactga gacataatca aaaatcaatg tgtcttcaaa cgtacctgta
    15301 agatatgttt tgccgttgat gcctgctgta atgttatagg aatgatcttc tccggaacct
    15361 ttaagcgtat tgagccaaaa caaatttcca ttcggattaa attttccaaa aaccacatct
    15421 gcatttccac ttccataaat tggagtaaca gtcaatccat catcaacaaa agaagaatca
    15481 gtaaatgagc ctgcaaacaa aaccaaataa tctttgtagt caattcggta aacatcatcg
    15541 ttcaatacac caccaacacc tctcacccat tgcaacaatc ctgttgaatc atactttgca
    15601 ataaaaccat cttgctgacc tctgtttgta gccatcacag caattccctt aggattaaaa
    15661 tttgtattgg tgttaaacac accacataaa taaatgttgc catactcatc aacagcaggg
    15721 tctgagtaat taaactcctc tcctgctcca cctacattaa ttgcccatat aaaattacct
    15781 gcactatcat atctagcaag aaaaatgtca ttaccaccat tgcttgttaa agaataattg
    15841 cttgtgctcg gatcaaaatc tgcagttcct gaaaaataac ctgacacaat aatatttcct
    15901 gtgctatcta atgcaatccc cattgcattc tcgaattgtg ttcctcctac acgtttggca
    15961 aacttatatt gctgcgcctc aatatcagaa gcaaacagac aacttaaaat gaataaaata
    16021 aaaaaattca ggcatttttg ctgttgcata acagttactt ttgttttatc aggtaaagat
    16081 atgtaatcag ccaatcgttg tctgactttc aaagcatatt tttgcataaa ctatggatca
    16141 ttcaacaaaa acttttgcac tatcaaacgg actacagata gttcttacaa ctttaagtaa
    16201 cacagaagtc tcgcattgtg cattactcat aaaagccgga acaagaaatg agcaaataca
    16261 acaagaagga ttagcgcatt tcatagagca catgctgttc aagggaacac gaaagcgtaa
    16321 atcgtttcat atcctaaatc gtcttgaagt cgtaggtggt gagctaaatg cattcaccag
    16381 caaggaagag acatgtattc atgcatcagt taccacacct catcttgaac gtgcacttga
    16441 acttattagt gatatcacct ttaattctgt tttcaattta aaagaaatag aaaaagaaaa
    16501 ggaagttgtt tgtgatgaaa taaggtctta tctcgacaat ccgggagagc agatttttga
    16561 tgactttgaa agcttagttt ttaaaaaaaa tccattagga aatccaatct taggtacaga
    16621 acaatcagta aaaagtttta agcagaaaga cctgattgcg tttacacgaa agttctacat
    16681 tccatccaac atggtgctat caattgccac atcattaaat gcagaaaaag taatcaggca
    16741 attagaacat catttcggca agtacagact tgccaaagtg ggcgaaagac caaaaccatt
    16801 taaaaaatac actgctgaaa agtcatctgt taaaaaacac agtgcacaat gtcattttat
    16861 tatggggaca ttagcacctt cactctatca ggcagacaga atgactgttg ccttgttgaa
    16921 taatattctt ggtgggccgg gtatgaactc aaaactcaac ttaggtgttc gtgaaaaata
    16981 cggttatact tatgccattg aatcaggttt taactcctat acagacacag gtatgtttca
    17041 tgtctatctg gctaccgata aaaaattcct gaataaaagt attgaccttg ccgtggtgga
    17101 aatgaaaaaa cttactgata ataaaatagg atcattacaa cttagtcaat acaaacagca
    17161 actaaaaggt caactagctt tggcaaaaga aaacaagtcg aatgtggtta tttctaatgc
    17221 ccgcagttta ttaaacttta agaaagtatt taccttagaa gatacatata agaaaattga
    17281 ggcagtaact tcttctcagt taacagaagc tgcagcaaaa tatctgaatg caaaatcgcg
    17341 cagctacagc agtctgcttt atgaaagcgt tggtcatgaa caataaaact gattcatttt
    17401 taacacgtat tgttgatgaa cttattgcca cagttgatgg aaataatttt gcagactata
    17461 ctgttgtatt tcctaccaga agagctgcat tgctttttaa attgcaactc gcaaaaaaat
    17521 taaacaaagc tgttttattg ccttcagttt tatctattag cgattttgtt gaacattaca
    17581 tgccactgcg agttgctgaa aagaaagaag ttcttcttga tttatttgct gtttacaagc
    17641 catttttccc tgattatgac tttacttcct ttgcaccttg gggtgaaatg attcttggag
    17701 attttgaaga aatcaatctt tacttagtta attacaaaga cctgtttgct gacctgacat
    17761 catatcatga aatagaatcg gtttttcagg atgatgatga atttaaagaa cagttgaaac
    17821 agttttttga attgttcagt acagataaaa cttctactat aaaacaaaac tttttatatg
    17881 cctggcaggc attgccgaag ttatatgaaa aactgaatga ggttttatat aagaacaata
    17941 agactacagc cggcaaagca atgcaacaat tagcattgtc gcctgaaaca tatttaaaaa
    18001 atctggattg cagtaagact attattgccg gattttatgc actggcacct gcagagcaaa
    18061 aattatttga atacatctta aatggaaaag gaaaaatatt ctgggatgcc gatgagtatt
    18121 acacaaaaaa tacaactcag gaagccgggc ttttcttcag aaaaaatttt ctaactcaga
    18181 atagttttaa atggcaagaa acctatttca aagatatacc taagaacatt aatgtaacag
    18241 gagtgcctct cattacagga caatttaaat ttctggctga tcgtttaaaa accttgattg
    18301 ctcagaataa actcaatact gataagacag ctattgtttt acctgatgaa aaattactgc
    18361 ctttgttgtt gcaatcttta cccgaagaga ttgagcatgt aaatatcaca atgggattcc
    18421 cgcttaaaga aactcatttg tatcgtttta ttgaaacact aaatgtttta ctcaatcaaa
    18481 tttcagaaac ttcaaagcag aagtttgttt caacctttgc attaggaaat tatcttgctc
    18541 acccttatgc ctcattgctg aagcacgaca tccgtaattt gaatgatgca ggcattagca
    18601 ttccgttgaa tgaacttcct gaaaacatca gtcagttttt aaaatcacag attacctctc
    18661 ctatatttga gggttatatt caattgttaa ccatgcttca tgatgccatg gaacctaagc
    18721 atcatgaaaa agaaattgcc ggatttgtat tgaatgaatt gcttgagctt cgtactatgg
    18781 taataccata tacagaaatt ctgaatgagc aagctttgca aatgctaatt tcagaaatac
    18841 ttgacaccac aagaattcct tttagtggcg agcctgtaaa aggacttcag gtcatgggat
    18901 tgttggaaac cagaacgctg gattttgaaa atatattttt ccttaatgtt aatgaaggct
    18961 ctgttccaaa atcaaatcgt catcattcat ttattccatt taccatcaga aagagttttg
    19021 gactaccatt gctcgatgag caggatgcta ttaccgccta tcacttctgg cgattgatgc
    19081 aaagagcata tgaaattgat ttaatttaca acacagaagt caatgagttt gccagtggag
    19141 aaagaagccg atttctttta cagttgtttt atgaaatgaa attacatttc agcaattggc
    19201 aggtcagtca tcaaattatt accacaccgc tttatcgtta taattacatt actcctggag
    19261 tcaaaagaga tgagcagtta acaaatcatt tactatctat ggtggcagag aataaattga
    19321 gattttctgc ttcatcgcta aacagtttta ttcattgttc attacaatat tactttaaag
    19381 aaattaaaaa aatcagagag cctgatgata atggggaaga gattgatgca gccgtattcg
    19441 gaaaaatttt acacgatgca gttagcaaga tttatgctca gattattgat gttgaatttg
    19501 acaaagacaa tgcagcccaa ctcaagaaat ctatttcagc atttgtaaaa gcatccatag
    19561 cccaagaata tgacaaacac tatcagggaa caggatatac tgtaatcatt gaaaaaataa
    19621 tttcggaata cattgagcgt atcatagcag ccgatctgaa aagtgaaact ttttcgcctc
    19681 atgaattaga aaccgaaaaa gtggttgttt gtgatattgg caatggtatt aatgtaaaga
    19741 ttaaaggaat ttttgaccgc attgacaaag tttctgatgg atacagaatt gtagattata
    19801 agacgggaag tgatgttgta aagaatataa aaaaacctga tgagcttaca agtgagccaa
    19861 aatacaaggt caattttcag cttttgcttt attgctggtt gtttttaaaa tcgcacagta
    19921 atgaaaaagt aaatgccgga atttatccac tcaggcgaat ggaagccggt atagaaatgc
    19981 tggggcagca acccttaaca gatcattcaa agtttgaaga attgttgttt gctttaatca
    20041 gaaaaatttt attggagtcg gattttacaa tgacaactga caccttacga tgtaagtatt
    20101 gtccttacaa agatatctgt aacagataat cacatcagca atttctcaac taagcggtca
    20161 acaggaaatg ttgctacttc gccattaagt tcttttaaag gtttccagaa aaaatgaata
    20221 tcatttgcat cttcaggtaa aatgaaattt atgcatgata ctttgtaata aacgctgatg
    20281 agttgtaagc cgggattgaa agccgattca ataaaaaaat cagtggtata aaaatgctcc
    20341 atcacttcta ccctttgctt tgtttcttct aaaaactcac gcactaaaca ttcgcgcaaa
    20401 ccttcaccaa attcaagtcc gccaccggga aatttagtaa attcaatacc gccttctttc
    20461 tcatgtgtaa gcaacacttc cgaattctta tttatcagaa taccataaac acgaactaca
    20521 aatggtttca tcgtttcaaa attagtcaaa aaaaaaggtt gacccttaga tcaaccttat
    20581 aaattattat tctctaaaat tattttgtga caattaccaa atattttgaa ttactccaga
    20641 acaaatcggg attagtaatt ttcaaagatt gatatttttt cttgtctttt tcataggtat
    20701 aagccgatgc aggatgagac gacataattt tcacactctt cgtatttaat tctacagaag
    20761 caaactgcct gatgtcaact tttgtgaatg ctgaattatc aatatcaggg tttatggttt
    20821 ttgttttacc gataccaata aatccacctt ttttattcag aatttttttg tcgcgtagtt
    20881 gtttgtatgt tccaattgca taatatgctg tgttaatctg attaactttt tcttcaatag
    20941 tcttttcgcg ctcaccaata gttgagttta acgtttcgat gttggtacgc aggccggtaa
    21001 tttcaatttt agcgtcacca agttgattgt ttaaattagc aatcatagaa tcacgttcag
    21061 caattttcag attcagtgag gtaatcattt tattaagttg ggcacttttt accccactgc
    21121 ttttaagttt tcttgtaagt tcggcaatgc gctgactgtt ttgatcgagt agtgaagcaa
    21181 tgctctccat attcttacgg atgcggtctt taacggttcc acccatttca ggatcacgct
    21241 tgcggtcaag tgtaatggca gcatgaatct gattgatgct gtctaaactt gcttcaatac
    21301 cgttgaagtc gccaaggaat gaatcaatag tgctgtcttt tagaaaagat gtgttaatca
    21361 aagagtcgcg ttcacgattt agtctatcta cttcgggttt gtagtctttg caggcataca
    21421 ctgccaaaaa cagaaatacg acagaaaatt taaggatgtt tttcatttaa tgaattttta
    21481 gtggttctaa tagttagtta ttcaagaagg attgtaatag ctgcaaatat agatgcaacc
    21541 agtcattaaa acaccatcaa ataaaaggga tttaaacaaa gatattcaca cttaacaagt
    21601 tctgacagta caggttaggc caacagatac aatttctacc tgttcctgcc tttcaatcca
    21661 accggtttct gcaacagcat catacactct gccatcaacc ataattttcc ctgacggtct
    21721 aagtgcagta aatgccgttc cgcgcatacc ggcttgaatg gtgtcaatat ctgtatgtgc
    21781 tctgaaacca tgttcagatg gttgtgttgt ttgaagtgtc aacttgctga ggcgctttga
    21841 gtcattaatt tttccaataa aataaaatat cagaaacact gcaccaagca tggctccaag
    21901 gcttattaaa atactttgtg ttatttgttc ggaacttgtg aaagtaaaat cccatccctt
    21961 gttgttgagc atacttaaag caaagcccat tgtagtacaa attattccta caatgccggc
    22021 aacaccaaaa cccggaatga caaaaaactc aagagccaat aaaatcacac cactaacgaa
    22081 aagtaatatc tcccaattgg cagcaagccc ttcaagatat aatggtgcaa aatataagac
    22141 tgctgcaaca aaagaagctg caatcggaaa aatggttccc ggagcctgaa attcaaaata
    22201 caaaccggca agcataataa gaattaataa actgctcaaa gcaggatgaa ttaaaaaact
    22261 aatgatgttg tcaacccatg aaggttgaaa ttcaataatc ttatcgctat tcagattttc
    22321 taactttaaa atatcagctt ctgtttcaac aatggcatta caatatttat ttgtcaaggc
    22381 ttcttctgct gtaaaagtca gcacccttcc actatcattt actcccggaa taacaactct
    22441 tggatcaacc atagcttcag caatacgcgg gtcacgattt cgtttttctg ccgttgcgcg
    22501 catgatgcca cgcatatagg attgatattt atccggtgct gcttcgcctt gctcagtaac
    22561 tacagtggca gcaccaatgc ttgaaccact gcgcatatag attctgttgc atgcaataga
    22621 gatgagtgca ccggcacttg cggcattagg gtcaataaat actacaaccg gaatttttga
    22681 atttaatatt gcaattctga tactgtcggc agcatcaagc atgcctccat aagtattgag
    22741 gtgaatcaaa aacaaatcag ccttctgctc atgcgcttcc tgcaatgctt tactgaaaat
    22801 gcgtgccatt cctctatcca cttccgactg aaagttaaac ctgtaaacaa tgccggcatt
    22861 aacagttgta gttgcaaaaa acaataacac taaagctaaa aataattttt tcatttcatt
    22921 taagttgaat attcaaaatt gcacaattac tacttacaac tgtcgttttg atgttaatta
    22981 atttgtttaa aacctattaa agcatcttca tttacgagga agtgattttt ttagtattgt
    23041 aaaaataaaa gtgattggga tgaaaatcaa agtttcgtta ttaattgcaa cagcagcact
    23101 aagcatctcg actgtaattg cacaggaagg tttcagaact attgcaggca aaaaatgtca
    23161 gctttataaa gttgtttcta aagatacctg gtcgggttta tccagaaaat atcacaaaga
    23221 aattgatgag ttaaaagcag ttaacccggg tataaacgat ttaaaaatcg gacagatcat
    23281 taatattccg attgaaaata cagttaacga caaaagcgaa aacaaatcgg atacttctgc
    23341 tgaaataaaa atgcgtaagc ctattgctgt aataaatgct ccggtaaaaa acaataacaa
    23401 ggttcatgtc attgaaaaag gagaaacact ttttggtata gcacgaaaat atgatgtcag
    23461 tcctaatgat attcgcaaat ggaatcatct tgataacgat ggcataaaag ccggtcagaa
    23521 attgattgta tcaagtacag aaagtgtagc aataaaaact gaagttaaaa aacaggaatc
    23581 taaaaatgag actatccttc agcctcttga tgacaaaaag ttagaagcaa aaaataatga
    23641 agttaaaaac gactcaaagt taattgcaga agcaagtaat aaagtaattc caccatcgcc
    23701 atcaccgagt gagatggaat acaaacccat gcgaaccgaa gtaaaacgaa gcaatggaaa
    23761 atcaggcgct gtaattgagg taaacgaatc gggcatggca tcctggatca gagatggaga
    23821 tatcaatcaa agtaaatttt atgctttaca tcgcaatgcg cctcctggca caataattaa
    23881 agtcaccaac agaatgaatg gcgattatgt ttttgtaaaa gtggtaggta cattacctga
    23941 cactggcgac aatgacaagc aaattcttaa aatgtcggaa gctgctgcaa aaagaatagg
    24001 tgcaataaat gaaaaatttc aggtcgagct taattacggc atgaccaatt aatagaaaat
    24061 gttgttttat aatacattga cgtacgcact gccaggcaaa aagtatattg gttctcctct
    24121 ataccggaac caacagtaaa ccctgtcgga ttttacaagt tacttttctt atttttgcgg
    24181 tttataatta aaattctgca taatgaaaga agtatatatt gtatcggcag tgcgtacacc
    24241 aatgggaagt tttggtggta aactgtcatc attttctgct acccgtttgg gtgctatagc
    24301 cattaaaggc gctattgatc gaatcaaact aaacccatca gaaatcaaag aggtgtatat
    24361 gggtagtgtt ttacaagcca atttaggaca ggctcctgca cgtcaggctg caaagtttgc
    24421 cggcttgccc gacactgttc aatgtactac aatcaataaa gtttgcgctt cgggaatgaa
    24481 agctgttatg ttagcagcac aaagtattat gcttggcgac aatgatgtga ttgttgccgg
    24541 aggcatggaa agtatgagca atgttccctt ctatatgcaa aatatgcgat ggggaaataa
    24601 atatggagat gtttctgctg ttgatggtct tgctaaagat ggactgacag atgtttatca
    24661 taattatgca atggggaatg cagcagattt atgtgcaaga gaatgtaatt tttcacgtca
    24721 ggatcaggac acctatgcaa tacaatcata caaacgcagt gccgatgcct gggctgcagg
    24781 aaaatttaaa gatgaaattg ttcctgttga aattccacag cgcaaaggcg atcctattct
    24841 ttttgcagag gacgaagaat ttaaaaacgt tcatttcgat aagattccgg gattaaaacc
    24901 ggctttcagc aaagaaggaa cagtaactgc tgccaatgct tcaaccatga atgatggtgc
    24961 agctgcattg gttttaatga gtaaagaaaa agcagagtca ttgggactta aaccccttgg
    25021 gcgtatccgc agttatgccg atgcagagca ggcgcctgaa tggtttacca ctactccatc
    25081 aaaagcatta ccaagagcag tagataaagc cggattaaaa atgactgacc ttgagtttgt
    25141 agaacttaac gaagcctttt cagtagttgc cttagccaac atgaaagaaa tgaatcttga
    25201 tcctgcaaaa gtaaatgtca atggtggtgc agttgcatta ggtcatccat taggatgttc
    25261 aggtgcaaga atattggtta cgttattaaa tgttttaaaa caaaacaatg caaaaattgg
    25321 cggtgcagca atttgcaatg gcggtggcgg tgccagtgct atggttattg aaagaatgta
    25381 atactacttt aatattagca ataaaagagg ctatctcaat tagagatagc ctctttttgt
    25441 tagcagaagt cactaaccta tttcaaaata tcaatacggg ctttgcaaca ctttatcgtg
    25501 ttttgtctgc ttaaaatcat ctttagaaat tgcatctttc ttaccaagat acgaccattt
    25561 tattgctgct gtatattcat tgaatgtatt attcaaatct ttcagcttaa cattgttaac
    25621 catatcagat aaggactcat ctaatttcca tgagcctgtt gcttcactac gacccaaggc
    25681 acctgactgt gcctcagatg tttctaattt ctgataatag ttagtcagat aaccctgacg
    25741 taaatccttc aactcttttt ctgtaaaacc tgttttcttt atagagtcaa taattgaaac
    25801 cattacatca atagcttttt taggatctgt agttgtgatg tagatatatg aatatggtga
    25861 tgaaactgca ttgcgcatat atccggcagc cggtgcataa gacaagctgc gttttgttct
    25921 aagctcaaca aaaaatctgt cgtaaagcat ttgcattgct aaatccatct gaacaccttt
    25981 ctctgttgac aaagaaggag cactcataat tcccatcaga tagtttgtgg caatatcacg
    26041 gtcaacaata acttcacttc cttttgttat taccaattta ggttccacaa ccggtgctgt
    26101 gcctactgcc atttttccta atgtcgcctt tattttaccc actaaatctg cttcgttaac
    26161 atcacctaca acaactaagt agcaacgctt ttttccaatt acatttttgt aatgacttat
    26221 aacatcagcc aaggttattt tctcaaggct tgcaacagag ccttgtggat ctttagcata
    26281 gtttctgcct ttaaaggttt cttccatagc gatattgctc aaagaaccat cggcatcaga
    26341 ctcgcgttgc ttagctcctg acaatgcttt ttctttttct aaatcaaact ctttagagtc
    26401 aaaagctgga ttcattactg catcagcaaa aagattccac gattcattcc aattaggttt
    26461 aatgcatgtc atactcaggt ttccataatc cagtcctgat gatgcttcga atgatgtacc
    26521 aattttttca gctgcatcat taaaggcaac tttatccaat ttcttggtac cgccattcat
    26581 agcaatagaa aatgcaaggc tctctacacc ttccttatcc ttcgtataat ttgcagttcc
    26641 tccttttaca taaagtctta cactaataac ttcttttggc gtatggcgta atattacttt
    26701 cagaccatca acattaactt cttttgttgc ttgtgcaagc gcaacaccat gtattgataa
    26761 cattgctgca cctattgcaa aatatttata taacttattc atagttcgat tttttatttt
    26821 aaaatttcat cagcagaatt aattcctaaa gactccttca ttttaggatt caagagcaga
    26881 cctgttacag aaggctttcc tttgatatat ttatctacat agtctgcaat atcttttctg
    26941 gtaactttct ttatcatgtc attgtaggta gtatagtagt caatgcttgc agatgcccac
    27001 caaaatgtaa cagtatgcac ataggctgat gttttttctt tgttataaat atcatccatg
    27061 atgagtttat tcttagcagt ctctaattgt tcgtcagtaa aataatcagg tgcatcaaaa
    27121 cgatttacct gctccatcaa cttaccataa aaagatttca tcttcatagg attaggaacc
    27181 ataaataact gaattggtcc tgtgtagcga caagtctgat agcctaaacc tgcctgaaga
    27241 gcataaccac catctaccaa agttttttga aattcagaag atgtcagtcc aagaatagta
    27301 gaaaacacat cggctgcata agttgactta acatcatttc ttgtatcagg cccatgaaat
    27361 cccatcatca taattggcat ttttgcattt ttatcaacgg tcaaaaactt tgactggcct
    27421 actaatggtg caaactcagg tataggccac ttttgaaacg gatcaaaatc acaaggtttc
    27481 cagtcaccaa acattttctc tgctttttca aaaacggcat tgtgctccac atcaccggca
    27541 acaactaaaa tagagttatt gggatagtaa tatttatctt ttattacatt catctttgct
    27601 ggtgtagcag aaagaattac gtcatgaata ccgatggcat ttttacgcac ataatttgaa
    27661 ccccacattt gtttgttaaa atcctgaaac agaaaaaagg caggatttga ttctgcacgc
    27721 tgaaattcac catcaacaac aggattttcg ttcttcattt cctgctccag aaataatgga
    27781 taacgaatag cattattcat aaactcaaga ccttcattga tgagtttgtt acttagtgtg
    27841 aaaaagtagt tcacacgctc ttcagaggta gtaccgttaa atgatatacc gagttcattt
    27901 actctttcaa gaaatttttc ttgtgacgga attgccttgt ttgctttaaa gaacatgtgt
    27961 tcgtaaaggt gtgacaatcc attgaaggcc gaatcttcag taaaagatcc attctttaca
    28021 caaatttcaa tagtgactaa tggaacagag ttgtcttcaa tgaccaagac ctcaaggcca
    28081 ttgcccaact ttttcatatg aaagttggaa ggcagatttt tttgtgccat tgctgtaccg
    28141 gctacaataa aggcagtagc aaaggctgtg atttttttaa gcatagttat acatttaata
    28201 gtaggctaag aacgctattt ctatagcccg tgatggttaa aaaaaagtta tgttattaat
    28261 taaatttaat tgtttgtggt attggtaaca atgaatttct gagtcttaat gtccgaatca
    28321 aaataaacag ttgctgtata aactcctgat gaatagtttt ttacatctaa tgaatagctg
    28381 cgaatcaaac ttccgggagt aacgtcttca accttttttc cagtaatatc cttaatttca
    28441 atacgattgt atggcgttga tgcctcaatg gcaataaaat ctgaggcagg atttggcatc
    28501 atctgtaatt tagcatgtga agcgtttact tcatttaagc caacaaatgt aaagtcactt
    28561 gtatatcgcg ccatagcaaa attattaacc gtacctacaa cggcagatcc ggcaaggata
    28621 attttagaat caggttgctg tactgcaaca gcatttgctc cggaagggtt accaaatgaa
    28681 gttatgttat aaccaatcat gttgttaaaa accaaatcga aagaacctaa tgtatcaaaa
    28741 cgcatagcaa caaaatcacc ggcagcgcct acataacttg aacctgcacc taccagataa
    28801 tcaccctgag gagcgataga aaaaataata tctgttcccc ctaaatcaaa aagagaaatg
    28861 ccgcttacac caaagttatt atcaatctga ccattactta agaaacgcat cagcaccatg
    28921 tctgtgtttg aaccggtctg tgttgtgcct ccggcaacta tcattccatc aggatttata
    28981 tatattgtat taatgatttc tgacatcgag cctatggtat agattgcttt tccatttaat
    29041 ccaaaattcg catctaatgt accgttggta ttatatttaa ccaaagcata agttgaaagc
    29101 gttctgcctg caagaacaat cttttctgtt ccaggttcta ttgccaatgc atttgcctga
    29161 tcccattgtg tgcctaatgg tgttctaacc ttgccactgt ttccaaagcc accatctaag
    29221 gttccatttg tattatatct agcaacagca aattcgccat tagccaatgt atcgaaagaa
    29281 acacctccca aaacaatttt accacttgaa ggaactattg ctacaccgta agcaatatcg
    29341 aatcctaaat ctaatgctgt ctgcacaata ccgttagcac caaactgatt gtcaagtgca
    29401 cctgctgtgg ttaatctggc catagcaaaa tgcgaatatg tttgattttc aagattaccg
    29461 gcaacaataa ttttaccatc attttgtatg gcaatggagc gcgctatact gttacctgtg
    29521 ccgatagaaa aagttatctt cccccctgtt ccaaactgat tatcaattgt accatcagca
    29581 agataacggg caactgcaaa acgattaaaa gttccattat tactttcacc agctacaaca
    29641 atttttccat ctgactgcac agcaattgct ctggcaacac aagcaaaact attaaacgat
    29701 gaagttactt taccatttac accaaaggta gaatcaataa taccgggata ctgaatctga
    29761 gccattgatt gtagtgtaaa aaaagaaaga cctgcaatga ggcatgtgtg tagaaatttt
    29821 ctcattctga atgtttttta cttatgatgc aaatatataa tttagcctga aaggcaattg
    29881 ttagttggca attatttctt atagcttaag tgtaatcagc aatttggcat agtctgttct
    29941 tgatctttga tgattcaaaa tgtcaataaa atataataaa ctgccctgcc caatttaatt
    30001 aaatctgtaa ctttgagcaa taaaattttg cacgtaaaat actatgtcac aagttgaact
    30061 gaagatgcct aaaatgggcg aaagcgttgc tgaggcaaca attaccaaat ggttaaaaaa
    30121 agaaggagat agagttgagc aggatgaacc tattcttgaa attgcaacag ataaagttga
    30181 ttctgaagtt ccttccacag cagcaggaat tttagttaaa caattatata atgaaggcga
    30241 tgttgttgcc gttggaaaat ccattgctat tattggtgat tctgcgtcag ctacagtacc
    30301 aacctcatca gtacctaagg cagaagaagt taaaattgca gcggctccag taattgcaac
    30361 tcaagctgca actgtggcta ccactgttag tgcatcttca aaattctatt ctccactagt
    30421 aagaaatatt gcacaacagg aaaatgtttc atctgcagag ttggatgcaa ttaccggaac
    30481 cggtgctgaa ggtcgtgtaa caaaaaaaga tattcttcaa tttattgaga acagaaaatc
    30541 aggtaatgga caaataacac aaactactca agcgcctact gtgcaacctg caacaacacc
    30601 gcaagctaaa gcgcctgctg caactccaat gactgatggc gatgtagaga ttattgaaat
    30661 ggacagaatg cggaagttaa ttgcagacca catggtgatg tcaaaacata ccagcccgca
    30721 tgtgacatca tttgtagagg ctgatgtcac caatatggtt ttgtggcgca ataaagttaa
    30781 aaacgatttt gagaaaagag agaaagaaaa aatcactttc actccattgt ttattgaagc
    30841 aattgtgcgt gccataaaag attatccaat gattaactgt tcacttgatg gcactaaaat
    30901 acttctcaag aagaacatta atattggtat ggcaactgca ttaccaagtg gcaacctgat
    30961 tgttccggta ataaaaaatg ccgacagaat gaatcttgtc gggctaacaa agtcggtaaa
    31021 tgatttgagt ggcagagcgc gtaaaaacaa actttcgcct gatgaaattg caggtggaac
    31081 ttttacattg accaatgtag gcacttttgg aaatgttatg ggtacaccca ttatcaatca
    31141 accacaagta gctattatgg ctgcaggtgc cattcgtaaa aaacctgctg taattgaaac
    31201 tgaatttggc gatactatag gtatccgtca catgatgttt ctctctttat cttatgacca
    31261 tagaatagtt gatggaagtc ttggaggtag ttttctccgt aaggtagcag actatcttga
    31321 gcagtttgat gttaacagaa atttattata atttgttatg agatttgtat ttgccattat
    31381 tccaatactc tggctgaata ttgcaatagc gcaacagtca aagaaacctg taaaaaaaat
    31441 tgttttagga aaagatcagg tttccgaaat gaaaaaagca ggttctgaat atttcaagag
    31501 tgagaattat aagactgcat tacctattta tcagcaactt caggaaaacg atccaaacaa
    31561 tgctgagttt aattacagac ttggtctttg ctatttaaat accaatgtaa ataaaaaatt
    31621 agcggcaggt tttctgataa aagccgctga aggaaaagat gttccaaaag atgtttatta
    31681 ctatactggt cgggcacttc tcattcttga agaaatagat gatgctgtag atgcttatga
    31741 gaaatttaaa atagctcatg gtggaaaagt tagcccaaaa ctgaatctcg aaaacaatgt
    31801 ggaatggtgc tatcactttc gcgatatgaa gaaaacacca attgaagtaa gttttactaa
    31861 tctgggtaaa ttggttaatt cacctaacaa cgattacaga ccggtttgtg atatgttagg
    31921 taatgtagtt tatttcagct caaatagaaa aagcaacaca ggtgccattg ttgatggtta
    31981 cggagaagtt atttcagacg gttattttac taaaaaagat acaggctaca gtaaagcaaa
    32041 aaatcttgga ccaaacatta acaccatgta ttatgaggaa ccattgcaat taaattccaa
    32101 cagcgacaaa ttgttggttt ttaaagaagg tggcagcatt tcttctccat tatatacatc
    32161 agaactcaat ggaaaatcat ttgagaaagc agttgctata aacaaatttc ctgcttcaaa
    32221 agttgatggt gcctgcatga cagatgatgg aaaaacaatc tatttttctg cagaccttaa
    32281 aggtggtaaa ggtggtaaag atatttggat gacaacttta aacagtaaaa acgaatggtc
    32341 taatccggtg aatcttggtg atgctgtaaa tacaaaatat gatgaagtaa acccttggtt
    32401 gttttttgat aatcagactt tgttttttgc ttccgaaggc cataatagta ttggcggatt
    32461 tgatattttc ttcagtacaa agccgcatga atctatggac tggagtgctg ctaaaaatgt
    32521 aggctatcca ttaaatacta ctgacgataa taaatatttt tctttaacag ctgattgtaa
    32581 aaccggcttt gtaagtcaga ctactgaaca tggttccgga gagttggata ttgtaaaatt
    32641 tgtttgcacc aacccaatca ttaaacaaga aaaagttgta tgtgatttta cttttttaaa
    32701 agctgatggt acttttgcac gtgatgccaa ctgtatgatt ataagaaacg atacaggtga
    32761 atcatcaggt gtgaaaacaa ttaatggctc aaacggcaaa ctttatgttt acctcggccc
    32821 gggtaattat aaagttaaag taaaaggcgt aaagtcaggt cgtgctgatg aggattttat
    32881 catcacaggc aatgaaccta aaatgcaatt ggtaaaaact tttaaattga cacctcccgt
    32941 taaagaagga aaataaaatg aatctgaaac tgcacagacc acttgctttt tttgatcttg
    33001 aaactacagg cgtagttata ggcatggaca gaattgttga attggggatt ttaaaaattc
    33061 tacctgatgg aacaaagaca aacaaaacat ggcgtataaa tcctgaaatg cacattcctg
    33121 aagtggtatc aaaaattcat ggcatttatg ataaagatgt ggccgactgc cctactttta
    33181 aagaagtggc aaacgatgta aatcactatc tcaataatgt tgaccttgcc ggatacaatt
    33241 caaaccgatt tgatgtaccc ttgctggttg atgaattttt aagagcaggt attgaatttg
    33301 acatacgagg acgaaagtta attgatgtac aacacatctt tcataaaatg gaacaacgca
    33361 cattgagtgc tgcatataaa ttctactgca atcaggagtt aatagatgcc cattctgccg
    33421 aagcagatgt gagagctacc tatgaagttc tggaagcgca attagaacgt tatagcgatc
    33481 agctacaacc tgacgcagcc tttcttcacg ccttttgtaa taacagtaag aatgttgatt
    33541 tggccgggcg tatagtttat gatgacaaaa agcgcgaagt atttaatttt ggaaagtaca
    33601 aaggacgctt ggtgactgac gtttttaaaa ctgatcctag ctattatgac tggatgctta
    33661 aaggcgattt ttcgagagat actaaacaag ttgtaacggc attgcgatta cgcgaaagag
    33721 aaaactaaat atatgtttga cctgtttgat tatttctgct gattaaaatt tgcaggtaat
    33781 ataaaaatct gtacttttat gctgatgggt tccgacaata attcagataa aaataatctt
    33841 ccttctgttt ctgcttcagg taaaggcagc aaagttgtta ttgaccttga agctgagaaa
    33901 aaagaaatca gtaaacgcta taggcattta cttagagcat gcaaatcaga tttagataaa
    33961 aaagataaaa aacttatcag gcttgcattt gatactgctc ttgaagctca taaggaaatg
    34021 cgcaggaaat caggagagcc atatatctat cacccgattg ccgttgcaca gattgttgct
    34081 gaagaaatcg gacttcgttc tgccatccct gtagtttgcg ctttactgca tgacactgtt
    34141 gaagatactt atcttacatt agaagatata cgtgatttgt ttggccctgt tgcagaaaaa
    34201 attattgatg ggctgacaaa aattgcagga gtttttgatc attcaagctc tttgcaggca
    34261 gagaatttca gaaagatgtt acttacatta agtgttgacg tcagagtaat tctcatcaaa
    34321 cttgccgaca gacttcacaa catgcgtaca cttggtagta tggcacgtga caagcagttg
    34381 aaaatagctt ctgaaacagc atacctatat gcacctttgg cacatcgtct tgggctttat
    34441 aatgtaaaaa cagaacttga cgatctggca ttgaaatata cagaacccga agtatataaa
    34501 acacttgccg aaaaactaag agactcaaaa aaagaacgcg acaaatttat ttcggaattt
    34561 attcatccga taaaagatga gttaaataaa caaggtttca aatttgagat taaaggaagg
    34621 ccaaaatcta ttcattccat ctgggagaag atgaaaaagc aaggagtaac ctttgaagaa
    34681 atttatgacc tatttgcaat aagaataatt attgactcac cacaaaatca ggaaaaagct
    34741 gattgctgga aagtgtattc tattgttact gatagttacc gaccaaaccc tgacaggctg
    34801 cgcgattgga tatcaacacc aaaatcgaat ggctatgaag cattgcacac aacagtgatg
    34861 agtgatcagg gcaaatgggt tgaggtacaa attcgttcac aaagaatgaa tgaaactgct
    34921 gaaaaaggtt ttgcagcaca ttggaaatat aaagagtctt cgcaagagtc ggccttggat
    34981 gaatggctta cacgtatcag agaaatgctt gaaaaccccg acagtaatgc tcttgatttt
    35041 gttgatgact ttaagcttaa tctctttgca gacgaaattt ttgtttttac acctaaaggt
    35101 gaattaaaaa ctttacccgt caacagtact gcattagact ttgcttatga aatacacacc
    35161 gatataggca gcagatgtat cggagcaaaa attaatcatc gtttagtacc catttcacat
    35221 aaactgaata gtggtgatca ggttgaaatt ttaacctccg acaaacaacg accaaaagaa
    35281 gactggttaa gttttgtgat tactgcaaaa gctaaaggca gaataaaatc atcattaaaa
    35341 gatgaaaaga gaattcttgg tgaacaaggc aaggaaatat ttgaccgtaa aataaaacat
    35401 cttaaaattc ctgtagcaga agttaacatg aatgatatac ttctctatta caaacttccg
    35461 aatgttttgg agttgtatta tcgtatagcc atggatgctg ttgatctgaa acagttgcgc
    35521 gaagccgtta attataaaga ccaacgtgtg cgcagtcagc gtacagaacc tactttagaa
    35581 gagttggtaa cacaggcacg aggctcgtcc gatatgcttg ttattggtga aaacctgaat
    35641 aaaatagatt ataaactttc accatgctgt aatcctattc ctggtgatga cgtttttggt
    35701 ttcatcacca ttaatgacgg aataaaaata catcgtgtaa actgccccaa tgccatgcaa
    35761 ctgatgagca actatgctta tagaattgta aaagcaaaat ggaccggtca ggagcagctt
    35821 tctttcttgg caggtataaa gattacagga atggatgatg taggtgttgt aaataatatt
    35881 accaaggtta tttcaagcga actgaaagtg aatatgcgtt ctatctcaat cgaaagtaat
    35941 gaaggtttgt ttgaaggaac catcatgctt tttgttcacg acaccgaaca tctgactaaa
    36001 ctgatgaaaa agctttctgc acttagcggt attttaaatg taactcgaat tacttaatca
    36061 tgtacgtatc ttttgaccaa ttaccggacg actcccgtat ctggattttt cagtcggata
    36121 gattattatc agaagaagaa attgaatcta tcaatccaat acttaatttt tttatcagtg
    36181 actggacagc acatcaacaa actctaagag cctcattcac ttttatgcat aatcattttc
    36241 tgattattgg agttgatgaa aatatggcta ttgccagcgg ctgttctctc gataaggttt
    36301 ttcatgttgt tcgtgcatgt ggccaaaaaa ttaatactga ctttttaaac cgtctgcaca
    36361 ttgcagcaat gattcaaaat gttattacgc ttcactctat aaaacaaata ttggatattg
    36421 cagaaaacag aaacaataat gagccattaa tgatattcag caacatcatt gaaaccaaaa
    36481 gtgaaatgaa aaataactgg ctaataccaa ttgaaaaatc atggcttgca ccaaagttaa
    36541 aatcaggaag taaaatttaa ttattaaaac tattttatga attttataat caaactcatt
    36601 atctctactc tggcagttat gatggtgtca tacattctgc ccggtgtgca tgttcaaagt
    36661 tttggcattg cacttatggt agcatgtgtc ttagcatttt taaatgcatt gataaaacca
    36721 atacttgtac ttttcacact acctattaca ctcatcacat taggtttatt tttgctggtc
    36781 attaatgcaa tcatcattat cataaccgac aggctaattg atggatttga agtaagcagt
    36841 tttggtattg cacttctgtt ctctttgata ttaagcattg ttacttctgt aatggagtcg
    36901 cttgccggac aaaatcaaag acaataaaaa accggcaact ttaaaagtgc cggcttatgt
    36961 gctgtcaaat aaacattagt ttcctaaaaa ctttgaaatc ttttcaaaat aaacattttt
    37021 atcgattgta aaattttctg cattggatgg agaatttttt acaacatagg tttcactgac
    37081 acctttaatt tgttttacat ctgttggatt tatcagatta tcctgagatg aaacaataac
    37141 cattaatccc ttaatgcctt taggtttttc catagcatac attggctcat gatttttgtc
    37201 aaagccaaaa ggcattacca cctctttacc cgttttatct tttatttttt tcttcattac
    37261 ttcaaggcca atccatggac catcggcaat aactttctgt gtttctgttc tgtttgcacc
    37321 aacaccaatt gaaagtcctg cgccaatagc tttaccatat aaattaaact ttgtgatggc
    37381 gcgcgactta cgtaagtaat cacaaacagc attaaggtca acaataaact gaggatagat
    37441 aaataaatcg gggtcaattt taaaatcgct gctgctgcca taaccacgat aatcatacat
    37501 ggctacatta tatcctgcac ttaaaaatga attagcaatt tcaatattgt cggccatatt
    37561 gccatcacca ctgccactca taattatcca gttggtagat tttttcggca actcaaacat
    37621 ccatgcattg agaattgcac catcaggagt tttaattttt tcttctttat aagtcattcc
    37681 gtacttatca ggaagtacct tatattcctt tgaaggattt agtgcaaaag cacctgaagc
    37741 aaataccgcc aatacggcaa ctaatgaaat tgatttaagc atcgtaattt gattgttaaa
    37801 tttaagcttc aaaaataagt acactatcca tgaataagta attaattttt taacaattct
    37861 tgccatttca ttgctgataa aatctacttt gcggcaaaaa aacaaatatc tgcaattatt
    37921 tctcacttag aataacttaa cgatagcgat cgggataatc tgtaataatg ccattaacac
    37981 cgctgtattt cagctttaaa aatactttgt catcattcac cgtccaggga ataattttaa
    38041 tttcttgctg tttgcaatat gctattgcct ttgcagttgc caatacatac caagggctgt
    38101 aaattgttgt cacaaaacca agttcattaa aaaaagccgc cacgtcccta ttcgtttcat
    38161 tcaaagctgc caaagtatat tcaggataat catcatgcaa aatacgcaac acttcaggat
    38221 cgaatgactg caatacaaat ctttctttct cgccatactg ttgcacaacc ttcatcacca
    38281 aatcaactaa ctgctttgtt tcaggctgaa atatgcctat ccaactcttc ttacttttta
    38341 tttcaatgga ataatatggc aacactcctt ttgtattaca acaaaaagta tcagcagtgt
    38401 taattacttc agaaagcaaa ggtttaccta caaccatttt atgttgtccg ggaaactttg
    38461 cataaaactt actaccgcaa tcataacctt ttacctgttc ataaggcatc ttgtaaatac
    38521 tgtaagctgc tcttttaaaa tccggcaatg gtgtactgtc aggcatcagg cagttttcgt
    38581 tatttatcca gggctcatgc gaaaccacta cctgagaatc tgccgataca acaacatcta
    38641 attcaagatg caaaacattt agctcaatag ctttcagaaa tgcaggaatt gtattttcag
    38701 gcattaaccc acgacatcca cgatgcccat aaactgtgaa agatgtgtct tgtgacatga
    38761 gatggatact aaataaagaa aaaagaaaaa ttaaaaatag aataatacgc acaataacag
    38821 ttcattatta actaaaaata aaaacgaggc aatcttgaga gtgccccgta gtagatgttt
    38881 tcacaacgga ataatcctta ttgtgatttg ctttcaaaaa caaaattagt ttaaaatttg
    38941 attccctcca aattttagca gttaatttaa aaaatattcc ttaattttat tcgcaaaatc
    39001 atatttataa gtactgaaat gaaaaaaata ctaggactag atcttggcac aaactcaatt
    39061 ggctgggcat tgatagaaca aaattttgat aagaaagaag gtagtgtcat tggaatggga
    39121 agcagaatca ttccgatgtc tcaggatatt aaagatgaat ttggaaaagg aaattcagtt
    39181 tcacaaacag cagatagaac gaaatttaga agtgtaagaa gattacgtga acgacatctt
    39241 ttaagaagag aaaggttaca tcgagtatta aatgtattag gttttctacc tgaacattat
    39301 gcaaatgaca ttgattttga aaaacatttc ggaaaattta aagacgaaac tgaaaccaaa
    39361 ttggtttgga agaaaaatgg gaaaaaaccg aatggaaaag ataagtttga atttgctttt
    39421 caatcaacat ttgaagaaat gttgaacgag ttcaaaaaat cacaacccga cttattgaat
    39481 aagaaaaaca aaaaaggtga agaatttaaa attccttacg attggacgat ttattatttg
    39541 cgaaaaaaag cactcacaca aaaattagca aaagaagaat tggcttggtt gattttaaat
    39601 ttcaatcaaa aacgtggtta ctatcagttg cgtggtgaag aagaggaaga aaatcaaaat
    39661 aagttggtag aatttcattc gttgaaagta gtagaagtaa aagcggaaat aaaatacatc
    39721 ttaactttgg aaaacaatga aacctactac cgtttaagcg aaacgcctct attggattgg
    39781 aaagatacaa accgcgactt cgttgtaaca acctatttgg aaactgacgg aacagtaaaa
    39841 aaagataaag acggaaaaga aaaacgtagt ttgtttttga aagatgattt agtttttatc
    39901 aaaaagaaaa caacaaaaat tactaaatca acaaaaaata aaaatgaaac ggttgaagaa
    39961 aaatcaataa agattattga tgtaaaagaa gaaaaacaaa atgaaatttg gtattcgtta
    40021 atattagaaa acggctggat ttatcgaaga caaagtaaag tttcattaga ggattggaaa
    40081 ggcaaagtgc gtgaatttat tgttaccacc gatttaaacg atgacagttc aataaaaaca
    40141 gatgacgaag gaaaagaaag tcgcagtttc cgtgcaccga aagaagatga ttggacttta
    40201 ataaaaaaga aaaccgaaga tgatctaagt aaatacattg gagaatataa aacaaaaaat
    40261 ggaattgacg gaactgtcgg cacatacatt tacgataatt tgcttcgaaa gcccaatcaa
    40321 aaaattaaag ggaaattagt tcgtacgatt gaacgcaagt tctacaaata tgaattaaga
    40381 gaaattctta aaaagcaaat tgtactgcag cccgaattgt tttcagatga cttgtataat
    40441 gattgtgtaa gagagttgta taaaaacaat gaggcacatc aactgcaact aaataaacga
    40501 gattttattc atttgttttt agatgacatt attttttatc aacgcccttt gcgaagccaa
    40561 aaatcaagta ttggtaattg ttcgttggaa tttcgattac ataaaatcaa taagaaagac
    40621 gataagggaa attcaataaa aaatgagttc gaaaaagacg aaaatggaaa agatattgaa
    40681 gtaaaagaat acttaaaagt cattccgaaa tccaatccgt actatcagga gttccgcttg
    40741 tggcaatggt tgtacaacct gaaaatatac aagaaagaag atgatacaaa tattacggct
    40801 gattttatta caagcaatga cgataaaatt actctttttg attttttgaa cagcagaaaa
    40861 gaaattgagc aaaagccttt gattgaattt ttggttaagc aacgggaact gaaaaatcct
    40921 gcaaacaata atttaaacaa aaaagtacaa acagcactaa gcaaaaagat aaaagaagaa
    40981 acagaaaaat accgatggaa ttatgttgag gataaaaaat atccgtgcaa cggaacagga
    41041 acgcaaattc gcacacgatt agaaagagta acaaacgttt ctgttagttt ccttacacgc
    41101 gaaattgagc aacatctttg gcacatcatt tattccgtaa ctgataaaaa cgaatttgaa
    41161 aaagctttaa aatcattttc gaaaagacat caaatagatg aaacttcgtt tgtagaaaat
    41221 ttcaaaaatt tcccaccatt tataagtgaa tatggtagtc tttcagaaaa agcaataaag
    41281 aaattattgc ctttgcttcg cataggtaaa tattggaacg agaatgcaat aaacgataaa
    41341 attaaaaaaa gagccaattc cataagtgaa cgattaaaat caatttcaaa aaaagaagat
    41401 atagaaaaca tatctgacga tgatattcaa aaacaaattt tgaaaagttt ttacggttta
    41461 actgatttaa cacaaggctt acaattatat caagcaagtt atttgattta tggaagacat
    41521 tctgaagcaa gtgatctaca gagctggaac tcggttgccg atttggaaaa gtatctcaaa
    41581 gaatttaaac agcattcgtt acgaaacccg attgtagagc aagttgttac cgaaacactt
    41641 cgtgtggtaa aagatatttg gacacattac ggaaaaggtg tagaaaattt ctttgatgaa
    41701 attcacgttg agttagggcg tgaaatgaaa aatacagctg aagaccgcgc agtcctcacc
    41761 aaacaagtta ccagtaatga aaataccaac atgcgtatta agttgttatt ggctgagtta
    41821 aaaaacgaca caactattga gggcgttaga gaatattcgc cctcccagca agagattttg
    41881 aagatttacg aggacggagt attaaattct aatgttgaaa ttccgaatga tatttctgaa
    41941 atattaaaaa aattcaaaga aagtgatatt aaaaaacaac caacaaaaac ggaagtcaat
    42001 cgttataaat tatggttaga acaaaaatat cgttcgcctt acacagggca aatgataccg
    42061 ctttctaaat tgtttaccga agcatatcag atagaacaca tcattccgca aagccgttat
    42121 tttgatgata gtttcaacaa caaagtaatt tgtgaggcag ccgtaaacca acttaaagac
    42181 aaacaacttg gtttggagtt tattaaaaat cattcgggac aaattattga tttagggcaa
    42241 aaccaaagtg taacactttt tacagagcag caatacactg attttgtaaa ggagcactac
    42301 gacaaaaacc gttcaaaacg aacaaaattg ttgttggacg aaattcccga caaaatgata
    42361 gaacgccaat tgaatgatac acgctacatc agcaagttta tttcatcggt gttatcaaat
    42421 attgttcgtg ccaacaaaga cgatgacgga gtaaactcta aaaatctttt gcccggaaac
    42481 ggaaaaatca cttccgaact gcgacaagat tgggggttga atgatgtttg gaatgattta
    42541 attctgcctc gttttgagag aatgaatgaa ttgtcacaaa gtaaccattt tacggcaacc
    42601 aataaagaag gacacaccat tcctgctatt ccattggaac tgtctaaagg tttccaaaaa
    42661 aagcgtattg accaccgtca tcatgcaatg gatgctttgg tcatagcgtg tgcaactcgt
    42721 gaccatgtca atttattgaa caatcaatct gccaaatcag acaatagtcg ctatgatttg
    42781 caacgtaaac tgcgaaaagt agaaaaatgg actgacaaag aaggaaaaga aagagataaa
    42841 ttcactgatt tctacaaacc ttggtcaaca tttacacaag atactaaaaa cgaattagag
    42901 aaaattgtcg tcagtttcaa gcaaaactta agggtaatta ataaggcaag caaccactac
    42961 gagaaaatta tcaacggtaa aaaagttttg gtaaagcaag aaggatcgaa ttgggcgata
    43021 agaaaaccca tgcataagga tactgtttcg ggactggttc aattaaggaa aacaaaaaca
    43081 gtttctttaa gtgtggcttt agagaattat aatgacatcg ttgacaaaac tttacgtaag
    43141 catattttat ctctaattaa tcagaattat gataaaaaga aattaacaaa atacttcaaa
    43201 gacaatgaaa ataaatggaa taatagagat atttctaaat tagatatata ttacttggaa
    43261 aatgacaatg ttgcatccag agttaattta gacatatcat ttaataaaga aagaattgaa
    43321 gataccataa cagatactgg tattcaaaaa atcttattga aacatttaat caattacgaa
    43381 ggacaaacag atgaaaaagg aaaagcaatt gcacctgaaa ctttagcttt tacaccagaa
    43441 ggaattgacg aaatgaataa aaacatcatt actttgaata acggtaaatc tcaccaacca
    43501 atcctaaaag ttagaacata tgagcctaaa ggaaataagt ttaccatagg cgaaaccgga
    43561 aacaaaaaag ataaatttgt agaagctgcc aaaggaacca accttttctt tgccatctat
    43621 gaagatgaaa atgggaatag aagctatgaa accattccat taaacattgt cattgaaaga
    43681 caaaaacaag gtttgtgttc cgttcctgaa cagaatgaaa aaggacatgc tttacatttc
    43741 catttatcac caaatgattt ggtgtatgtg ccccaagtag atgagaatct ggatgcaatt
    43801 aactttggtg agttgacgat agagcaatcg aatcgaattt ataaatttac tgatggtagt
    43861 gggataatgg caaattttat accttcaaaa attgctgaag ttatttttaa tataaataaa
    43921 ttaaaacaag aaagtttgaa aattaactac cctattcaaa atgaaattgg tgtaggatca
    43981 caaggctcaa aaaatgaaag agctatatct ggtgagcaga ttaaaagtat ttgtattaaa
    44041 ctcaacatag accgcttagg caacattact ccttcaaaaa aaactaatca gaaatcaaca
    44101 tcaagttttg taaatgaaga acattctatt tactacaata aagttacatt caacaacctt
    44161 caacaatccg atgaaaaaat gtttatgcat acagccaaca tgaatcctga agagcgtatg
    44221 gcgtatttgc aaaaactgcg tgagacaaca caagaaaata tttctgaagc agaaaagaga
    44281 gccattttaa atcagataaa aataaatccg ccagatgaaa atacttgatt tggaacaatt
    44341 gcaacaaatt catcgtttaa aaaacaagaa atcgtgatga gataaaaaac aaatgattaa
    44401 acgtacgctc tattttggca accctgcata cctgagctta aagaacagtc agctaatggt
    44461 gcgtttgccc gaggtggaga aaaaccaaca acttgatgtt gcgtttaaac aacaggcaca
    44521 tgctaccatt cctgttgaag atattggtat tgtattgctc gaccatcaac aaattaccat
    44581 aacgcatgga ttactcgatg cattaatgga gaacaatgtt gccgttataa catgcaacaa
    44641 tacccggcat cctgcaggac tgttgctgcc actgcaaggg cacactttac agcacgaacg
    44701 atacagtgat cagctttcgg ccagcgagcc tttacggaaa caactttggc agcaaaccat
    44761 tattcaaaaa attaaaaatc aggcagcagg attacaatta caaaatattg attccacacg
    44821 cctctaccct atctacaaca atgtaaaaag tggcgatagt gagaatgccg aaggtgttgc
    44881 agcagccttc tactggtcaa atatttttac aaaaattcct gctacaatag aacatgcacc
    44941 gggtaattta ttcaaacgac aacgtgatgg tttgccaccc aacaattatc tgaattatgg
    45001 ttatgcaatt cttagagcaa ctatggcaag aagtattgtt gctgcagggc tcttaccaac
    45061 aaaaggtatt catcatcata accgttacaa tgcctactgc cttgcagatg acctgatgga
    45121 gcccttcaga ccttttgttg acagaattgt tattgacatt atcttaaaac atggtataat
    45181 tgatgacttg acaaaagcta ttaaagcaga tttattgaca attcctgtaa tagat
//
LOCUS       DAOOOI010000012        43637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_143_length_43637_cov_31.422674, whole genome shotgun sequence.
ACCESSION   DAOOOI010000012 DAOOOI010000000
VERSION     DAOOOI010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 43637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43637
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143_length_43637_cov_31.422674"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PKX59_03400"
     CDS             <1..517
                     /locus_tag="PKX59_03400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ89945.1"
                     /translation="KEAVAEFRKKYSIPDKPIVYIGNAHKQKGVYEAYHALKDLDVEL
                     IMTGAENNASDLPIRFLSLNREEYITLLHASAIIVLMSKLMEGWNRVAHEALLCNTPV
                     VGSGMGGMGELLTKSKQIITTNDAIKENVVAVLKNRNKYSNVGYDFVKQFDMNYFNSA
                     WQQTIENLIRR"
     gene            518..2413
                     /gene="asnB"
                     /locus_tag="PKX59_03405"
     CDS             518..2413
                     /gene="asnB"
                     /locus_tag="PKX59_03405"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOZ89946.1"
                     /translation="MCGINGVFNFNKVNIGDENALIGLMNDTIRHRGPDDSGISEVNQ
                     RLFFGHRRLSIIDLSKAGHQPMRNEVGYEIVFNGEIYNYAELKKYLPDYKFKSDSDTE
                     VLLGLYEKFGESCLQYLNGMFAFAIWDPRKEQLFLARDRSGKKPLYYSFQNGVFAFSS
                     EVKALLKLPFIKTELDEEAFYHFLTFNVLAPPMTMFRNIKKFHPAHSMTIEKTGHFRY
                     QPYWNIEYSDYSNLTEEELKTQTLERLQKSVQYRMVSNVPVGAFLSGGVDSSALVAMM
                     SKMTNQPVKTYSIGFEGQPDFDELEHARKVSEMFGTQHFEKTVNPDEFKNFLPKIVDI
                     FDEPLADPTCVPIYFISQKARQEGTIVVLTGDGSDEIFAGYRNHLKYIHNYKTFDKFS
                     KLPTSIKKLVSGTFSLINSNSPLSEMLSRAAQNQEFYWSSARSFKESVKSRFLHPDFR
                     NRHQSLNSYSIIKAFKEEYNAIKKPFHDHIDWMCFMGFKLTDPHWYLYRGDKLGMAHS
                     IELRSPFLDYEFVNFALSIPGSMKVKNNEPKYILKKSLEAILPDEVLYRKKMGFCLPL
                     KQWGGEIMIDYVESNYVEFCKNFNLLNQDGIKQILNNVKGGNERSVNDLWTAYFLISW
                     FKRWMHA"
     gene            2406..3551
                     /locus_tag="PKX59_03410"
     CDS             2406..3551
                     /locus_tag="PKX59_03410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOZ89947.1"
                     /translation="MREVKKVLIVTNVPQDYRIAMFNALNLLLQKENIQFKVAFGAAG
                     YKRRKSKLDFNKMKFDYTILESTKFTFGSTENTMFTYPGLLKLHKEYRPDMTFVIGYS
                     PATIKLWFYSFFMNINYFIWSGSVNFKGRKDSTLRKILRKILVKRAKGFIAYGTKAKE
                     YLVYLGAPENKIHIAINTVDTHFFNEIAKKRLNRKVNTQKQLLYLGYLTPGKKIDDLI
                     KTINELKNLRNDFVLRIIGDGPAIENLKSLTSELHLTDIIKFEGFKQKNDLPPFMEQT
                     DCFLFQTGCDIWGLVLNEAMAAGLPCIVSPNAGAARDLVFDTKNGLILDFGSHKIAAS
                     RINELLNDEVLLQKMGYSANEFIESNVTAEKSAQGFISAIKQYCSHA"
     gene            3544..4866
                     /locus_tag="PKX59_03415"
     CDS             3544..4866
                     /locus_tag="PKX59_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89948.1"
                     /translation="MLDFYLTIASYLGLAVIVIVGLFFNRHFFLTLFIVKPFIDMTVN
                     VSVIGDFNALEVSGVFSALILLIKYLNKPIAYPTFNEIWIWIFIGLQLLAFFLNVSAD
                     TRSIFISTKVYLKMLNSYLVYFLAAIEIFENFKQRVNLVKIVWLTALFAGLITILVFA
                     LGLSNFDTTKGVVRYNGLYNDPGTPSYLSVICLLFCNLYFKIAQKKPNHFFQFLRILT
                     LAVTAVILVITMTKSALLMFLVYVIMWYGIYERHLFLIVPALLISIYFSFSLSDDLNT
                     RFETEINYVDSGGDAEAAKSMGTGRVNRWETLLDVYFNELGPMNQLFGTSKHWMAHNQ
                     YIAYLMQVGIVGLTVFLIFLIRFIVKLYSIYHRTRNPSVYAAFTLLIMYVFYAFTGHP
                     FDYTTLLWYLMILLSMINVYEVHQMRNKQQKLKAITDKNFLLNKTQLN"
     gene            4878..5852
                     /locus_tag="PKX59_03420"
     CDS             4878..5852
                     /locus_tag="PKX59_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent 4,6-dehydratase LegB"
                     /protein_id="HOZ89949.1"
                     /translation="MKNVLITGAGGFIGSHLTEQCVEAGYNVKAFVRYNSKSNYGWLE
                     GSKYASQIEFITGDIRDYDSVHHAVKGCDTVFHLAALIGIPYSYVSPMAYIKTNVEGT
                     YNIVQSCREHGTENVLVTSTSETYGTAQYIPIDEKHPLVGQSPYSASKIGADQIALSY
                     FLSFNTPVKIVRPFNTYGPRQSARAVIPTIITQLLSGKSDIKLGHITPTRDLTFVKDT
                     ANGFITIAQSSKFTGSVTNIGMNNEISVGDLAKLIAEMMGKKINIITDAQRVRPDNSE
                     VERLYCDNTKLITNSNWKPAYNIQSGLKETIDFLEKHLHLYKPDIYNV"
     gene            5862..6566
                     /locus_tag="PKX59_03425"
     CDS             5862..6566
                     /locus_tag="PKX59_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOZ89950.1"
                     /translation="MIAVLLAGGKGTRLAPFTISLPKPLMPVGNRPILEILIKQLKDA
                     GVTKVVICVGYLESLIRSYFGDGSKFGVEIIYSSETEPLGTAGPMKLIESYLTDSFFF
                     MNGDLFTDINLEEMFRFHKDNKAVATIGLTRRTVDIDFGVIKIDQTNEFTEWKEKPTL
                     EYLVSMGIYVFEPSVLKYLPDGFFNVPDLIIKLHEAKESVKGYQYKGNWLDIGRLEDY
                     QRACQQMEAIEKKSNG"
     gene            6559..7437
                     /locus_tag="PKX59_03430"
     CDS             6559..7437
                     /locus_tag="PKX59_03430"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ89951.1"
                     /translation="MANATIAITGAGGFLGKALTALLKKNNFEVVSISRSSGVDITKA
                     ETLTTIPDFDCLIHLAAHTFVPDSYNNTAAFFSINLNGTLNALELCKKRNARFIFSGS
                     YVYGQPNYLPVDENHPINMWNPYASSKIICEQLCHVYNKEFQVPVDILRIFNIYGPGQ
                     NSSFLIPKIIEGVFKGQLNLETLTPRRDFIYVNDVCNAFLQCLLIHSEEQTFNVYNIG
                     SGSSYSVQQIVDKVISFTGLNPTISCNEKRRAVEVEDVKADNSKIKRDKKWHPSVSFD
                     QGLKDMIEIYRNENDL"
     gene            complement(7448..7951)
                     /locus_tag="PKX59_03435"
     CDS             complement(7448..7951)
                     /locus_tag="PKX59_03435"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOZ89952.1"
                     /translation="MLVSIIAAMDQNRLIGAENDLPWHLPVDMKYFKETTLGHYVLTG
                     RKNYESIPEKFRPLKGRTNLIVSNSKIQFPGAILVSSIKEGIEIARKAGEKELFIIGG
                     GQVFKQSIHLANRLYVTLIHHQFNGDVFFPQINWEQWEIISRKDVVPDENNKYACTFI
                     VAERISV"
     gene            complement(7956..8750)
                     /locus_tag="PKX59_03440"
     CDS             complement(7956..8750)
                     /locus_tag="PKX59_03440"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOZ89953.1"
                     /translation="MNQYHELLRHVMTNGVEKSDRTGTGTKSVFGYQMRFDLQKGFPL
                     ITTKKLHTKSIIHELLWFLKGETNIEYLKQNGVSIWDEWADKNGNLGPVYGHQWRSWS
                     TPDGNGIDQISSLIDTIKNNPDSRRMLVSAWNVADVSKMALPPCHILFQFYVANKKLS
                     CQLYQRSADIFLGVPFNIASYALLVHMIAQVCQLEVGEFIHTLGDAHIYSNHFEQVEL
                     QLSRTPYQLPTLQMNPLVKDVFKFKFEDFYFSDYQSHPAIKAQVAV"
     gene            complement(8972..15916)
                     /locus_tag="PKX59_03445"
     CDS             complement(8972..15916)
                     /locus_tag="PKX59_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOZ89954.1"
                     /translation="MIRKHIIALLMFCTVFTVDVKASHLFGGEITWECIKSGPNIGKF
                     QFTLKVYRDCNGIPFTPPSAINVYGNPLVTSIPINAALTVITDLSPTGCGFTCANPAA
                     GATQEFVMKTNPVFLNGTPPASGWVFAIDDCCRNTLVNVGGGGQGFTIRAVMYPFNGQ
                     NMNPCYDSSPFFKERPNTYICIGYQYTYNPTAVDPELDSLYSDWTNPLEQPTSSPPAA
                     FNAPIINFTAPYTVNSQLPGNVTMNHNSGEVTFFVTTASSVTGNFATCLKVTAYKCQQ
                     KVAEIFRDYGVVLTTNCFVQNPPAINLPPAINLPFAGGTMKDTTVHVGDTVKFRIDAT
                     DFQRNIGSGAAFQTIRIDAYGDEFGVPINTNSGCGYPPCAKLDKLTPVISPLAAFINF
                     EWVTTCDHIIKPSGCLRERNTYYFIIKAQDNYCPAPAISTITIAITVVQSKPLEPPHV
                     RGASVLNTTGDVGLYWETPGVVNQLDTHHVFNSYQIYASNSYGGPYTLVDSVAGNKDF
                     YKQKGDTITATQLNTLIGANANAAPVYFYVRTKSLCNGDSISVPSDTISTMFVSTSIV
                     NCETQVNWNSLFKHAYPGSAALVYKIYREHPIGSPPVQIATVPNTWPNATYTDPFSKT
                     VCRDSVKYYVTIDDTLIIKSTPTVTRCVSKSNVTKQYIDNSFTASISPIGPTNLCPPN
                     CVNLTVSANPSPACFTTSYLWSNGATTSGITACAAGNYSAVITQSPSGCTSTTPIHVV
                     TLTPGPTANIAISGVSSVCPGDSVNLQFTFAGGAPYTYSYNVPGPGCVGTTVVNAVAN
                     SSPVTVRVPVNCNFNYTLNSVMGACAGTVSGSANATIKALPTASVVNTANDTVCNGGG
                     STISLAFTGTGPWNYVIKSSTGPDITATTASNPVSINVTPASTTTYTLFSVKDACTGT
                     VSGTRKIVVLPIPTAAMSITGNDTICSGSSTTIRVDFTGTGPFVGNIKDNTTGVLTPV
                     NTAASFVTLNVTPNATTTYSFQNFVSSAKACPGTIANSVVVAVKAIPTANISSVTNAT
                     ICKNDSVRMQVSFTGNGTPYTFGLVTNAGAPANVNAATNPYYFYVHPTTSTNYIVTTV
                     TDAFCSKSNINDTVAVTVNQLPTAVLSGTTTICAGQPTTLTINFTGTGPFTGSYVAST
                     GGTTNFNTSANPYTFNVSPSATTTYSLSATINDSKCSNNTNPATAIVTVNALPQASIS
                     GNKTICTGANDTLLVNFTSGNAPWTFYYKDGLGNTYGPVTTSNNPYKVVVSPTATTTF
                     QLDSVFSGVCKGSVNGIGSVTVRPLPTATFTTNNDTICNGNATNLTIQFTGTAPFSYQ
                     LQGQAVQNAATNPVTINVNPTTTTNYTLVQVNDQLCTSATSQQVKITVLNLPTAVLTT
                     TTPNLCTGVSGSITINFTGNGPFTTAYSNGTVNTPVVSNTNSIVIPVSPTVNTTYSLV
                     GNVNSMYGCNAPVNPATALITVRPLPTMTISGNPIICESTSANFNITFTGTAPFNYSY
                     FNGTSNVNGTTSTNPLTITVSPPVGTTTYTPVSIADQYCTGTALNGSSVVKVNPKPTA
                     TLFGNDTICVGSSSFIAIKFVGTPPFDYSYTTNGVSSGSLSSPVDSVSIPVAPNVNTT
                     YALSTSISDANCSNSNINQSVYMKVTPIPTAAITGTNAICSGGSTNLTINFTGEAPYN
                     YSYLAGGTTMGPFTTNNTSVTIPVSPTANTNYTLPATVNGNGCNGPASGNAMITVNAI
                     PQAVISTAKDSICKGDSTQLSIQFTGAAPFTYQLQGQGVTNATSNPHTFYVSPQSNTS
                     YVLTNITDNNCPAVINQNIGVKVLDNPTVAISTATPSLCKGNPGSITLNFTANGPYSV
                     TYNDGTSNFPVTSTSNSMTIPVTPTATTTYTLSGVVTGLLGCKTSATGATTVTVHELP
                     QAVISGNPVICLNEIAPIQIQFTGTAPFNYSYQNNVTGQINNGTTSQNPLTINLSPTT
                     TTSYNLTAISDKNCPGTQISGLANITVNPLPQPVITGVNQVCEGTPSVLSTTLPYQSY
                     QWSNATTGSTTAVTTTGTYTVEVTDINGCKKLSPPFAFIAHPYPVVNFTNDTSKTCQV
                     PRVNYFNQSLYQPGATFQWSLGENAVSNEVNPSHVYSQPGTYNVQLIVTNPFGCKDSI
                     EKQVEVIFYPLPVAQFTANPMVTNIFNGPVSFTDQSAYAVSWKWVFGEGDTLYDQNVT
                     HYFPEIGEFPVKLMIENISGCKDETQQTIVINPFWLPNAFTPNGDGRNEVFFDPGFAM
                     DYSGFNLRIFNRWGQMLFQSDSPSKPWDGSNGKGDLAPQGTYVYTLSVTNKGGKRHDF
                     NGTITLLR"
     gene            complement(16033..17301)
                     /locus_tag="PKX59_03450"
     CDS             complement(16033..17301)
                     /locus_tag="PKX59_03450"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOZ89955.1"
                     /translation="MNRVIEPELKLTNKVDFTLPEKIILDNGIQAYVLNAGEQEVAKL
                     ELVFMAGEWYGEFSMLASAVNELLESGTAKRTAYEISQSFDQYGAYLNTSCSFDTASI
                     KLFTLSRFIPETFSLLLEIMSEAVFPDEEVKIFTDQSIQQLKINSQKVDYISRKTFNQ
                     MLFPDNHPYGRPASEADYKKLNSADLVLWHKQYYQPQNCVIFLSGRFNEDAVKSLNQL
                     FGPWKDSTLSKIVEKNFSIEKYKPERKYLEKKDALQTAIRIGCHTPNKLHPDYIPLTV
                     LSTVLGGYFGSRLMSNLREERGYTYGVGCAMVSWLHGGYFFISTQVGKQHRENALEQI
                     YLEIKRLREEKIPDNELNRVKSYMTGVYQRSLDGPMLVSDRIKSMVLYNQDFSLLNSY
                     LQIMNSTDSNQLLDLANRYFSNKMTEVSAG"
     gene            complement(17298..18545)
                     /locus_tag="PKX59_03455"
     CDS             complement(17298..18545)
                     /locus_tag="PKX59_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOZ89956.1"
                     /translation="MVKFEKIILPNGLPVIVNYDPSTPLVAMNILYKVGARNEDAGRT
                     GFAHLFEHLMFGGSVNIPSYDEPLQRAGGENNAFTNNDFTNYYLTIPSQNVETAFWLE
                     SDRMLSLAFSEKSLDVQRNVVIEEFKQRYLNQPYGDVWLNLRPLAYKVHPYQWATIGK
                     DIEHIEDATLDDVKAFFHKWYCPNNAILTLSGNIKPELAFELAEKWFGEINAHELSKE
                     SLPKEPIQREYRNIMLERDIPANAVYKAFHTCSRFDNEFYATELLTDILCGSKSAVLY
                     HSLVKEQQLFNSINAYFTGDLDNGLMIVEGKVNDGVDVKHADHAIDTLLQLVVNDGVQ
                     EKEMKIVKQKTESTLVFSEVNIGNRALNLAYFEMIGSAEKINNQLQQYEVVTANNVQQ
                     VAKTIFKKENESSIYYCKKGVAV"
     gene            complement(18549..19493)
                     /locus_tag="PKX59_03460"
     CDS             complement(18549..19493)
                     /locus_tag="PKX59_03460"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOZ89957.1"
                     /translation="MKNRITELFGIEYPIVQAGMIWCSGWRLASAVSNAGGLGLIGAG
                     SMYPDILKEHIDKCKAATNKPYGVNVPLLYPDIEKLMAIIIAGGVKIVFTSAGNPATW
                     TTHLKQAGITVVHVVSNVKFALKSQAAGVDAVVAEGFEAGGHNGREETTTLCLIPEVK
                     DALQIPVIAAGGIGCGRAMMAAFALGAEGVQVGSRFVASIESSAHTNFKEKVINTSEG
                     QTVLTLKQLTPVRLIKNKFSEQVDLAQQRCATREELTQLLGRARAKKGMFEGDLDEGE
                     LEIGQVSSQINNIIPAGDIVKNIWNEFLIEKNRIANLF"
     gene            complement(19580..20746)
                     /locus_tag="PKX59_03465"
     CDS             complement(19580..20746)
                     /locus_tag="PKX59_03465"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOZ89958.1"
                     /translation="MINSFNCVLLFAFLFCGLFSDAQSLRQPAFVEYSFNAGRVVKNY
                     PIFPERKLAGINSIVIGSRLNGYKAWHPYYNFPNASFELVYGGLGNKKVLGNFSALLY
                     RLEFSYKMNDKFYTEITPSFGFAYFNKPYHAINNPENTLIGSAFTFCAAARLNLRYYI
                     NPFWSVSAGAGVYHCSNSHYQLPNLGINLPVASAGLRYHIRPLNLLFGNRSLEKDANY
                     HFQVRLAVGYNEQGASTFPVNGPRYPIYLGAFYVTRYLTPVNKLHLGVEGWYNSGVYD
                     FITSQRYYKDKEHLRSTALQAVVGNEFLFGNFSLLINGGIYFYNPVYHEKLRRENETD
                     FKKQLKGWITARIGFQYYLKDATIYHKNNFYAGVYIKTNLGQADFLETGIGYAF"
     gene            complement(20751..21506)
                     /gene="tpiA"
                     /locus_tag="PKX59_03470"
     CDS             complement(20751..21506)
                     /gene="tpiA"
                     /locus_tag="PKX59_03470"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOZ89959.1"
                     /translation="MRRKIVAGNWKMNGTLDDAIKLTSEIVEMYKDEINSDVQVVLSP
                     PFPFLNNVSKLTNSTAIKIAAQNCAAQASGAFTGEVSASMIASCGVGYVILGHSERRT
                     YFNDTDEVLLLKVKQALLNGLQVIFCIGETKTERESNNHFECVENQIKNVLSNLTSDE
                     FAKCIVAYEPVWAIGTGLTASSAQAQEMHYFIREVLKRQFGEQASETSILYGGSCNEN
                     NAKELFALPDVDGGLIGGASLKSRSFISIIKSF"
     gene            complement(21534..23714)
                     /locus_tag="PKX59_03475"
     CDS             complement(21534..23714)
                     /locus_tag="PKX59_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOZ89960.1"
                     /translation="MSEVRNPQVAIATEIYTEDSVLIGKYYHENRTPVSFDSISTYAV
                     QALVATEDVRFYKHFGLDLYALISGAYSTAKGDERGASTITQQLAKNMYSTRKKVNQG
                     LLQHIPLVGTLVSKSKEWITSVKLEMFYSKDEILEMYFNTVDFGNNWFGIKVAAQNYF
                     SKQPSELNLQESATLIGLLKATSSYNPLNNKKRSLARRNIVLSQMQKYGYISQAAFDS
                     ISPMPLNLKRPTNNPATKDSYLRQLIAKIIKSYCDENNLNINEDGLKIYTTINSHLQN
                     YAELAVKDHLRKVQKQFDEHWGKQNPWRDDNGREILNFANTAVAFTQSYDRLKKQFPS
                     APDSVNFYLNKKKSMKVFTYNGAKDTVMSTLDSLKYYARLLNAGTMSLNPLTGEIKAY
                     VGGVDFNFFKFDHVTQSKRQPGSTFKPFAYLAAIEDTFSPCSRLDDVPVSIEYDGGQI
                     WQPYNSTGAFTYSSKTLRRALAQSVNSITAQIAELVGWDKIVECAHRAGVKSRLDTVP
                     SICLGTSDVSVYEMTCAYAPFVNGGKSVEPILVKKICANDGTVLKEFMPEFKQVISEE
                     NAWLMLYMLQGTIQEPGGTSQALWGYSVFKNGNDVAGKTGTTSNYSDAWYMGVTHDLV
                     TGVWVGAPYRSVHFRNSSGQGSRLALPIFGKMLEKAYQDPKSGVVVGKFKKPKKISKT
                     IYCENDDIELEAADSLLNDSLINTELMILESDSLKSDSLQGGGD"
     gene            24023..25564
                     /locus_tag="PKX59_03480"
     CDS             24023..25564
                     /locus_tag="PKX59_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOZ89961.1"
                     /translation="MSLTEQFKEVVNKEYQFKGDSIVLGAPVLKGETLTGSFIKAPLK
                     TFNRHGLIAGATGTGKTKTMQGIAESLSAKGVSCLLMDVKGDLSGLASPGTTNPKIDE
                     RYQKLGLTWKPLQFPVEFLTLSDEKGVRLRATVSEFGPVLFSKILDLNDTQQGVVSLI
                     FKYCDDKQMPLLDLKDFKKVLQFLTNEGKDEIKADYGAISSASASTIIRKVIEIEQQG
                     AEKFFGEKSFEVEDLLGIDENGNGKLNIIRLTDIQDKPKLFSTFMLSLLAEVYSTFPE
                     VGDPDAPKLVIFIDEAHLIFNNASKVLLDQIETVIKLIRSKGIGVFFVTQSPEDIPAA
                     VLAQLGLKVQHAFRVFTANDRKALKLIAENYPLSDYYKVDELLTTLGIGEAAITVLNE
                     KGVPTPIAHTLLCTPASRMDILTPDELTKSISKSRLTTKYNETLDRESAFEVLSKKLE
                     TQVAENTKATKAAEAKEEPSQLDQVLNSSTGKQVMRTVTSVLTRSLLGALGLGATRRK
                     KSGWL"
     gene            25583..26407
                     /locus_tag="PKX59_03485"
     CDS             25583..26407
                     /locus_tag="PKX59_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOZ89962.1"
                     /translation="MTSPKSEWKYDLISTIFPSKFLKREIRIDYYLPAQYSKNTGVLF
                     MLDGQDAELLHLKDTLQSLTQLKKIKPIIVIAIHANKNRLQEYGVVGKADYLKRGNKA
                     KQFSKFILDELIAHCSSFTGIKFTPSKTAIAGFSLGGLTAFDLAWNHPSVFETVGVFS
                     GSFWWRKKSYEDGYTDDDRIMHQVVKKSKSAPAIKCWIQTGTKDETADRNKNGVIDSI
                     DDARDLIFEMKQKGVPEKNIQYVEIPEGQHNQITWSAALPLFLCWAFGNKRKVDIY"
     gene            complement(26408..27784)
                     /locus_tag="PKX59_03490"
     CDS             complement(26408..27784)
                     /locus_tag="PKX59_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOZ89963.1"
                     /translation="MRYHFIAIAGSAMHNLALALHQLGHEVSGSDDEIVEPAKTRLAK
                     SGILPEKTGWFPEKINADIDAVILGMHARADNPELAEAIQKNIKVFSYPEFLYEHSKN
                     KTRVVIGGSHGKTTITSMILHVLKTSGNNFDFMVGAQLEGFDTMVKLSNDAPIIVLEG
                     DEYLSSPIDRRPKFHLYHAQIGLISGIAWDHINVFPTLEIYEEQFRIFAREIPRDGKL
                     FYCMNDDAVRKICEAENVVADRKGYSIPKHVIRNGKTFIYDDYNKEVPLQIFGEHNLM
                     NLEGARMICNSIGVNNEVFYNAIKSFKGAARRLELLEENDQFAVFRDFAHSPSKLKAT
                     LEAVRRQFPERKLIACMELHTFSSLNENFLDEYKGTMADADVAVVYFNPKTIEHKKLP
                     PISSEKIFQSFDRSDIKVINDSMQLINFLNTEDKQQVVYLMMSSGTFDGLNLKSFAES
                     LNTSHQKV"
     gene            27922..29004
                     /locus_tag="PKX59_03495"
     CDS             27922..29004
                     /locus_tag="PKX59_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOZ89964.1"
                     /translation="MKAKILDLYIIKKFLGTFLFAIGLIICIAVVFDISEKVDDFIER
                     HAPLKAIAFDYYLNFIPYFANLFSPLFVFISVIFFTSKMAGQTEIIAILNSGISFNRF
                     LRPYIISATVIAIASFYLNNWVIPHANLVRLQFENIYIKNPYVYRGRNIHRQIKPGEF
                     VYFESYDNKANTGFMFSIEKFEGGKLVTKLMADRITWDSLTQKWSIENYTIRHINGMT
                     EELKKGIRLDTTLSIHPSEFNRRMNFIEAMDFSELNSYIKEEKSRGSEVIPFYEVEKY
                     RRTSYPFATFILTLIGVAISSKKVRGGIGLQLGAGILLSFTYIMFMQVSTTFATNGNL
                     PAVVAVWIPNLVYAFIAIYLLRKAPK"
     gene            complement(29055..30194)
                     /locus_tag="PKX59_03500"
     CDS             complement(29055..30194)
                     /locus_tag="PKX59_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89965.1"
                     /translation="MARKLKLRQRQKMSIMYKAIFVTAGTSIMGLAVYLTVVMNTSNV
                     TDSRAGALTNMIQGYDVNNGEVICAFDWNGDDPTKASKGPDAIKVSPFAVVMSEGVEG
                     TNGLSAGKGKDGIDLTIPTDNYFNTDGIDISFDYKRLDDNCDFISRGRYFNFGMKNGK
                     LIIAYRVNLEKGRSYSVNETTNYELVKDNDFRNYRFVYNPNTGKGEIMVDDVVVWNYQ
                     GPKERSMFWKNSDNIVIAKNMNGGGTDKTILDNFIIRSTTHVQQLPITLLSFQAEARD
                     HHVMVSWYTSRETDIDSFVVERSVDAIEYQKIGAIKANGNSEVLRAYAFVDKAPLENK
                     IAYYRLMPSNKPLKSIAVPIIGYRYRMNHPETAFNPADYPADTVK"
     gene            complement(30292..33375)
                     /locus_tag="PKX59_03505"
     CDS             complement(30292..33375)
                     /locus_tag="PKX59_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89966.1"
                     /translation="MSRIRIQFLVFSLIYFFAYCNAVFAVTKTTTASGSWSNPAIWSP
                     TGVPASSDDVVISSAHNITISQQTFCKTLVVSGGAVLSLSPSKLLQINGAVNIAGTFD
                     MNGGNVKQLLNNAALQIGVTGKLIWNPGDNTLTGASLLTNSVETFNETSTLEIKKWYS
                     YATVPLASVITGNLGNVTISTQVGSTLFEWNQNNQFESHQIKGTLTINNGWVVLDKSG
                     SISDTKIGKIILPNVNSVLEFHSGSHPASFKVTTDSIANIGGEITGLLNGEGNVTLNV
                     NGGFLNMGNVELIYNKGTLNTANGNAKLNVGGKYLQTHGDLRGVFNLSSTAAGNTEFK
                     FNSLEMTGGLLFAYYACNATGALNSVTVTNDMVLNFSNASDKFRVNGLTTLAGQYSKS
                     RSLLKVDGNLILSGNAAAEFTSSGSVGAEENRIKGDVNISGLNSNFNMGSHAVILSVN
                     GDVNIDGGNTCFSKTPGVSQISLNKNFSLQSGTFILKGHEGKSELTVNGAYSQTGGVF
                     YLHSNTLTASNDSVKAYFKSSFRQWNGIINFDDNPEGGKSVLFIQGNEFIAKGSSLIT
                     RAGTGIGSVFGNLIYCTNGDLNYQRSTASVIDQIKQRITVNCRLKVTDGNLLLSSHSQ
                     PAVDFLRVESGAILNLLSTQIKSNLKYQNSGFTVDAGGLVRTANSKGFYNTLGTAAVC
                     SNGNLNYFLDRNSIVDYVGSNQLITGSGSGVINKEQFKYGVLRVSGNGILATNVEVRN
                     RIELGSSTIKLNQKTLIIENENTNALSASGGSLICASESDKLIWKNMKGGSSYTIPFS
                     SDGKVVSNVVVQPIVSGDISMYNYTGNPVSENLPDGIQPFLVDGKDVTDESLARRIIG
                     FDSQVKANITLKFFKNEIPGFKNGDTLVLIKNIGQWEVTPFSKVGDEQNPVSVQATNI
                     NANAVFALCTGIGAKMPDNTNGNNNSQGNFSVEQISPVPFNDFLKVNINAPTIGVIYL
                     TIVGSDGRELRKQMFDVSPGKNLLALSELSSLPTGVYFIQINGFKQTITKKVMRQ"
     gene            complement(33362..33655)
                     /locus_tag="PKX59_03510"
     CDS             complement(33362..33655)
                     /locus_tag="PKX59_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89967.1"
                     /translation="MRKKRKKHLPNRLRQSAKLRLSYRLIAVTATVTMAVVVGGIIYF
                     NISNSKKSKASTTTSDAEQRPVDFNIPDLKQNFREETINGIRVRKMRDEHVTN"
     gene            complement(33747..34916)
                     /locus_tag="PKX59_03515"
     CDS             complement(33747..34916)
                     /locus_tag="PKX59_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOZ89968.1"
                     /translation="MAKIFIVEDDAMVATLIRQSLSKNPDFEIQHFESAEECLNNLHL
                     NPDIVTIDYHLPGMNGVGLMRRIKDYNASIMCILVSGQDSLEVVVDSYKSGAQDYIIK
                     NDNLFVNLENSVKNLSMNVVLKKENEMLKDQIIDRHKYSNIMGSSNAVLRILRLIQKV
                     EKSNMMVLVTGQSGTGKELVARALHYNSPRARKNFVTVNMSAIPEELIESELFGHEKG
                     AFTDARERRIGKFEEANEGTIFLDEIGEMPLSLQAKLLRVLQEKEIQRIGSNKTIKLD
                     FRMVAATNRNLAVEVKEGRFREDLFYRIQGFLIHLPPLKERGDDIILLAKHFLDLYAQ
                     ANKTPVAQLSNEAAKFMLDYEWPGNIRELKAVMERAAILADNATISVDDLMFVNL"
     gene            complement(34928..37279)
                     /locus_tag="PKX59_03520"
     CDS             complement(34928..37279)
                     /locus_tag="PKX59_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ89969.1"
                     /translation="MSYKKINLLVVDDDEVDRIAIKRAIKSSGFNADLHFGDTYDEGM
                     EKALSMEFDVLIVDYYLTVMNAGDFISEYFQRGGTAPIIVVSSQGEEKIVVELMKAGA
                     CDYIPKAVLTPETIARSLKGALKSKASKNNHSNIETALVQTERTLNAVVSKSPLILFS
                     INESGIFTLFKGMAMDTIRLSEEDVIGKSVFEIWNRLPVRLKDVKKALLGEEVKQNME
                     FEGRFFEVHYMPSVNSEGALTGIMGIATDITGHKEQEDELRQQIILSSEAQKMKEQFL
                     ANMSHEIRTPIHGIIGITKIVLQSQLDKDQSHYLNAIRKSADNLLVIINDILDFSKIE
                     AEKMTFESVCFNLFDLINTINELFKARVTDKSVKIIADFDQQLPKLLKGDPVRLSQVI
                     NNLMGNAVKFTHKGSVTLKTELINISDTKASIRFKVIDSGIGIAEDKLATIFDSFSQA
                     GTDITRKYGGTGLGLSIAKKIVELQDGSIRVESKLNHGTTFIFEMPFDLPAPGEVETE
                     AKKVAEVHAGFNGKIKILVVEDNDINRLIINKHIKDWGFDHDEACNGLEAIEKLSAND
                     YDVILMDIEMPELNGYAATERIRTEMKGHKSQIAILAMTAHVSASEKEKCIAAGMNDY
                     VSKPFDPTEVKNKIIELSGKANESVTEQPIEEAANSQSSSQATTKLTSLSFLNEMSGG
                     NADFIKEFLSLFLTNIPVSIDELEEGLKHKDFEKIRQSAHKMKPSLNYVGLKDTYETV
                     ATLEKYAREKSNLEDYPAMIKKISDECSVACIELESELKNVEV"
     gene            complement(37293..37727)
                     /locus_tag="PKX59_03525"
     CDS             complement(37293..37727)
                     /locus_tag="PKX59_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ89970.1"
                     /translation="MTIDIPPGIILVEDDDIDRMVVTRAFAKNNIKNPLHIARDGEEA
                     LEMLKGLNGKEKLDPLPKIMLLDINMPRMNGLELLREIRHDEELQTISVFMLTTSKDD
                     QDRLEAYKNNVAGYIIKPVSFDKLVEAIAVLNTYWNLSVLPA"
     gene            complement(37936..38496)
                     /gene="efp"
                     /locus_tag="PKX59_03530"
     CDS             complement(37936..38496)
                     /gene="efp"
                     /locus_tag="PKX59_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOZ89971.1"
                     /translation="MADTGDIGIGSVIRFNGDLYQVVEYQHRTPGNLRAFYQAKLRNL
                     KNGKLAENRFRSGEQVEVARVEYKTMQYLYKDGENLVVMDNETYEQHYIPEVMIGDSA
                     SYLKEEMMLKIAFEGDNPILAEPPTFVELVITYSEPGVKGDTATNTLKAATLETGAVV
                     QVPLFVNEGEKIKIDTRTGTYVERVK"
     gene            complement(38582..39031)
                     /gene="rplI"
                     /locus_tag="PKX59_03535"
     CDS             complement(38582..39031)
                     /gene="rplI"
                     /locus_tag="PKX59_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOZ89972.1"
                     /translation="MEIILKQDVKNLGYADEIVKVKNGYGLNYLIPKGMAVIASEANK
                     KVHTETMKQRAHKMAKIKGDAEKLADAISSVTLTIPVKVGENGKLYGSVTSQNIADSL
                     KKLGYSIDKKQIEMPEEHIRKTGTYSAAVALHRDIKAKINFDVVENA"
     gene            complement(39044..39316)
                     /gene="rpsR"
                     /locus_tag="PKX59_03540"
     CDS             complement(39044..39316)
                     /gene="rpsR"
                     /locus_tag="PKX59_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOZ89973.1"
                     /translation="MMSSKQGEIRYLNPVAVDTTKKKYCRFKKVGIKYIDYKDATFLI
                     KFVNEQGKLLPRRITGTSLKYQRKVGQAVKRARHLALMPYVGDLLK"
     gene            complement(39325..39693)
                     /gene="rpsF"
                     /locus_tag="PKX59_03545"
     CDS             complement(39325..39693)
                     /gene="rpsF"
                     /locus_tag="PKX59_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOZ89974.1"
                     /translation="MSNQYETVFILTPVLSNEQMNEAVDKFRKILTDNGAMIVHENNW
                     GLRKLAYPIQKKNTGFYYLIEFKSSGELINKLEVEYKRDERVMRFLTVALDKHAIAYN
                     EKKRKNAELKKKEQETATAN"
     gene            complement(39909..40469)
                     /locus_tag="PKX59_03550"
                     /pseudo
     CDS             complement(39909..40469)
                     /locus_tag="PKX59_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017260284.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     assembly_gap    40470..40479
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<40480..41237)
                     /locus_tag="PKX59_03555"
     CDS             complement(<40480..41237)
                     /locus_tag="PKX59_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017260284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ89975.1"
                     /translation="MPIFENILLALHAVKSQLLRTILTVLIIAIGIMALVGILTAIEG
                     LKASINENFTSMGANTFTIRNSGLGIRLGNSGKKAKRFKEITLQQAVLFKSGNTYPSI
                     ISISTMCNGVAVCRVGSEKTNPNILVMAIDENYLQTGGYTIATGRNMTQTEAMRGDNV
                     VMIGSEIKSKLFPGADAVDKEVYIDNRKYRIIGVLAEKGNSMGFGGDKVCLISLINGK
                     QNYGSDNRSWTVSVKVNHVSGLEAAVDEATGLFRT"
     gene            41736..>43637
                     /locus_tag="PKX59_03560"
     CDS             41736..>43637
                     /locus_tag="PKX59_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89976.1"
                     /translation="MKNQIQATALAIVICLFTFFTRAQSQSVNIALTLPTDTIGACES
                     NVYALSFSGASGARVVITASLSNAPPANCINASPIYLDFITASNNVSNVVTYNNGQTM
                     SFTVTNNNAVTLSYHAFIDCSVISDAPQGNSNINFIQTFTDSSATNYTLSVNNQGNVH
                     TSLNVQTPTLVALSSNLNQVAYYKTDSFLYFYYQNTGFTKLNFNFTFTPDATNYCQRI
                     TTGQPEYNIGLNGNFVATAPNTAMVLNIGDTLVVRQAVQAVACLDKIPACGPVAAQLQ
                     WTCNNNIPSNIFCTNCKNGIPVNYYINSGDAPVVKIERIAPADSLYDFSCLNDTVNFV
                     HWQYRVTNSGMGAMDSLFFNINQSFPTLNNALPNYNALSLIPFPSFSWQQNTGSLCTV
                     AVDTLHRSDWLCKNLVADALFEAAFRIKNFGNADTVIIAFNTLRCSEEDTNLFNVQKN
                     YNHWAFNSVKGRKVCGDGVSADYNIGLANTTSISTHAVGGSADVSQNLIFSPTVTDLS
                     VAPGATFGDSALLKVRLKGLLPSPYDYQLYGCSVPVNNCLMNGWLRAEVHCDTNLRIA
                     NPAMHGQLVYYRTSQGDTLVIKPDYVFAAVDTNLCEAGNYNYYFNMNSAGFKAFLDSA
                     HFEFTVQACC"
BASE COUNT        13658 a         8380 c         7421 g        14168 t
ORIGIN      
        1 aaaagaagct gtcgcagaat tcagaaaaaa atattctatt cctgacaagc ctatagttta
       61 tattggcaat gctcataaac aaaaaggagt ttatgaagcc tatcatgcat taaaagattt
      121 agatgttgag ttaatcatga ccggtgcaga aaataatgca tctgacttgc cgatacgttt
      181 tttatcatta aacagagaag aatatatcac cttgcttcat gcctctgcga ttattgtgct
      241 catgtcaaag ttaatggaag gatggaaccg ggttgctcac gaagcattac tttgcaacac
      301 accagttgtc gggtcaggaa tgggtggcat gggtgaactt cttacaaaat cgaaacaaat
      361 aataacaaca aatgatgcta taaaagaaaa tgttgtggca gttttaaaga atagaaataa
      421 atatagtaat gttggttacg actttgtaaa acagtttgac atgaattact tcaattcagc
      481 atggcaacaa acaatagaaa acttaattag aaggtaaatg tgcggcatta acggagtttt
      541 taattttaat aaagtcaaca tcggtgacga gaacgctcta ataggtttga tgaatgacac
      601 tatcaggcat cgtggtccgg atgatagtgg tatttctgaa gttaatcaac gcttattttt
      661 tggacatagg cgccttagta ttatagatct ttcaaaagcc ggtcatcaac ctatgagaaa
      721 tgaagtcggt tatgaaatag ttttcaatgg tgagatttac aattatgcag agttgaaaaa
      781 atatctgcct gattataaat ttaaatctga tagtgacaca gaagtattgc ttggactcta
      841 tgaaaagttt ggagaaagtt gccttcagta tctgaatggg atgtttgctt ttgcaatttg
      901 ggatccccgg aaggagcaat tatttttagc aagagatcgt tcaggaaaaa aacctttata
      961 ttattccttt cagaatggtg tatttgcctt ctcttcagag gtaaaagcat tgttaaaact
     1021 accatttata aaaacagaat tagatgagga agcattctat cacttcttaa catttaatgt
     1081 attggcccct ccaatgacta tgttcagaaa cattaaaaag tttcatcctg cacatagtat
     1141 gaccattgaa aaaaccggtc atttcagata tcagccttac tggaatattg aatatagtga
     1201 ttatagcaat cttacggaag aagagttaaa aactcaaact ctggaacgat tgcagaaatc
     1261 tgttcagtat agaatggttt ctaatgttcc tgtaggagca tttcttagtg gtggtgttga
     1321 ctcaagtgca ttggtggcta tgatgtcaaa aatgacaaat cagcctgtta aaacctactc
     1381 tatcggtttt gaaggtcaac ccgattttga tgaacttgaa catgcaagaa aagtttctga
     1441 aatgttcggc acacaacatt ttgaaaaaac agtaaaccca gacgaattta aaaattttct
     1501 gccaaagatt gtagacatat ttgatgaacc attggctgac cctacctgcg ttcccatcta
     1561 tttcatttct cagaaagcaa gacaggaagg tacaatagtt gtgctaaccg gtgatggaag
     1621 tgatgaaatt tttgcaggat atcgtaacca tttaaaatat attcacaact acaaaacttt
     1681 tgataagttc agtaagttac ctacaagcat taaaaagctt gtttccggaa ctttttcatt
     1741 gataaattcg aattctccat taagcgaaat gttaagtcgt gcagctcaaa atcaggaatt
     1801 ttattggagt agtgccagaa gttttaaaga atcagtaaaa agtaggtttc tgcaccctga
     1861 tttcaggaac agacatcagt cactaaattc ctattcaatc attaaagctt ttaaagagga
     1921 gtataatgct atcaaaaaac cttttcacga tcatattgat tggatgtgtt tcatgggttt
     1981 taaactaact gaccctcatt ggtatcttta tcgtggtgac aagttaggta tggctcattc
     2041 tatagaactt cgttctccat ttcttgacta tgaatttgtt aacttcgctc tttctattcc
     2101 aggaagtatg aaagttaaaa ataatgagcc gaaatatatt ctaaaaaagt cactcgaagc
     2161 cattttgcct gacgaggttc tttacagaaa gaagatgggc ttttgtttac ctttaaagca
     2221 gtggggtggt gaaatcatga ttgattatgt agaatcaaac tatgtagaat tttgcaaaaa
     2281 ttttaattta ctaaatcagg acggaatcaa acaaatattg aataatgtaa aaggaggcaa
     2341 tgaaagatct gtaaatgatt tatggacagc atatttttta atatcatggt ttaaaagatg
     2401 gatgcatgcg tgaggtaaaa aaagttctta tagttaccaa tgtgccacag gattatagaa
     2461 ttgctatgtt caatgcatta aacttattac tgcagaaaga gaatatacaa tttaaagttg
     2521 cattcggtgc agccggatat aaacggagaa aatccaaact cgatttcaat aaaatgaaat
     2581 tcgattacac aattcttgag tcaactaagt ttacatttgg ttctacagag aataccatgt
     2641 tcacttatcc cggactttta aaattacata aagagtatag accggacatg acatttgtta
     2701 tcgggtatag ccctgcaacc attaaacttt ggttttattc gttttttatg aatataaact
     2761 actttatatg gtcaggctcg gttaatttta aaggtcgaaa agattcaact cttagaaaaa
     2821 tactaagaaa aatattagtt aagagagcaa aagggtttat tgcttatggc accaaagcaa
     2881 aagaatattt agtttattta ggcgcacctg aaaacaaaat acatatagca ataaacaccg
     2941 ttgacacaca tttttttaat gagattgcaa agaaacgctt aaacagaaaa gttaatactc
     3001 aaaaacaact attgtattta ggttatttaa cgcccggaaa aaaaatagat gacctcatta
     3061 agacgattaa tgaattaaaa aatcttcgca atgattttgt tttacgtatc ataggtgatg
     3121 gacctgcaat tgaaaattta aaatcactaa cttctgaatt gcatttaaca gacatcatta
     3181 aatttgaggg tttcaaacaa aaaaacgact tgcccccgtt tatggaacag acagattgtt
     3241 ttttatttca aacaggctgt gacatttggg gtttggtttt aaatgaagca atggcagcag
     3301 gactaccttg tatagtttcg cctaatgcag gtgctgctcg tgatttagtt tttgacacta
     3361 agaatggact gattttagat tttggatcac ataagattgc agcaagtcga ataaacgaac
     3421 tgttaaacga tgaagtgctt cttcaaaaaa tgggatatag cgctaatgag tttatagaaa
     3481 gtaacgtcac agcagagaaa agtgctcaag gatttatttc agcaataaaa caatattgta
     3541 gccatgcttg atttttattt aaccattgct tcttatttag gcttggccgt tattgtgatt
     3601 gtcgggcttt tcttcaatcg tcatttcttt ttaacactat tcatagtcaa accatttata
     3661 gacatgacag ttaatgtcag tgtaattgga gactttaatg cattggaagt ttccggagtg
     3721 tttagtgctt taattttatt aattaagtac ttaaataagc ctattgccta tcctacattt
     3781 aatgaaattt ggatttggat ttttattggc ttgcagttgc ttgctttctt tttgaatgta
     3841 tctgccgata cacgctccat ctttataagt acaaaagtat atctgaagat gctgaactcc
     3901 tatctggttt acttcttagc agcaattgag atatttgaga actttaagca acgagtaaat
     3961 ttagtaaaaa tagtttggtt aactgctcta tttgcgggat tgattacaat tctggttttt
     4021 gctttaggat tatcaaactt tgatactacg aaaggtgtag taagatataa tggactgtat
     4081 aatgatcctg gaaccccttc ctatctttct gtaatttgct tattattttg caatttgtat
     4141 tttaaaattg cacaaaagaa acccaatcat ttttttcaat tcctaagaat actaactctt
     4201 gcagtcaccg cagttattct tgttattaca atgactaaaa gtgctttact catgttttta
     4261 gtttacgtta taatgtggta cggaatctac gaaaggcatt tatttttaat tgttcctgcg
     4321 ctgctaattt ctatatattt ttcgttcagc ttatctgatg acctgaatac ccgatttgaa
     4381 acagaaatta actatgtgga ttcaggtgga gatgccgaag cagccaaatc aatgggcaca
     4441 ggtagagtta accgttggga aactttactt gatgtttatt ttaatgaatt ggggccaatg
     4501 aaccaacttt tcggaacatc caaacattgg atggcacaca atcagtatat cgcctattta
     4561 atgcaggtcg gcattgttgg tttaacagtc tttctaatat ttctaatacg gtttatagta
     4621 aaactataca gtatttacca ccgcacacgc aatccttctg tttatgctgc atttacactt
     4681 ttaatcatgt atgtatttta tgcctttact ggtcatccat ttgattatac tacattactt
     4741 tggtatttaa tgattctact ttctatgatt aatgtatatg aagtacatca gatgcgaaac
     4801 aaacaacaaa aactgaaagc gataacagat aaaaatttct tacttaacaa aacacaacta
     4861 aattaataaa atatcagatg aaaaatgtac tcattacagg tgcaggagga tttatcggtt
     4921 cacatctcac agaacaatgt gttgaagccg gatataatgt caaggcattt gtcagatata
     4981 attcaaagtc aaattatggc tggcttgaag gctctaaata tgccagtcaa attgaattta
     5041 taactggcga tatcagagac tatgacagtg tacatcatgc tgttaaaggc tgcgatactg
     5101 tctttcactt agcagcatta attggcattc cttacagcta tgtttctcca atggcctata
     5161 taaaaacaaa tgttgagggc acgtataata ttgttcaatc ttgccgtgaa catggcacag
     5221 aaaatgttct tgtaacatct acgagtgaaa cttatggcac tgcacaatat attccaattg
     5281 atgaaaaaca cccattggtt ggacagtcac cctactctgc ttcaaaaatt ggtgccgacc
     5341 aaattgcatt aagttacttt ctttcattca acacgcctgt aaaaattgta agacccttta
     5401 atacttatgg cccacgtcaa agtgcaagag ctgtaattcc tacaatcatc acgcaattat
     5461 taagtggtaa aagtgatatt aagttagggc acatcactcc tacaagagac cttacatttg
     5521 ttaaagacac tgcaaacgga tttatcacaa tagcccaatc atccaaattt accggatctg
     5581 ttactaatat tggcatgaac aatgaaatca gtgttggtga tttggctaaa ctaattgcag
     5641 aaatgatggg aaagaaaata aatattatta ccgatgctca gcgtgttcgt cctgataaca
     5701 gtgaagtaga gcgtctttac tgcgacaata ccaaactcat taccaatagt aactggaaac
     5761 cggcatacaa tattcaatca ggcttaaaag aaactattga ctttctcgaa aagcatttgc
     5821 atctttacaa acctgacatt tacaacgttt aaattcaaga tatgatagca gttttattag
     5881 caggaggaaa aggcacacgc ttagcgcctt ttactattag tttaccaaag cctttaatgc
     5941 ctgttggcaa cagacctatt ttagaaatat taatcaaaca acttaaagat gcaggtgtga
     6001 ctaaggttgt tatttgtgtt ggttaccttg aatcattaat acgttcttat tttggtgatg
     6061 gctcaaagtt tggcgttgaa ataatctact caagtgaaac agaaccttta ggtacagcag
     6121 gtccaatgaa acttattgaa tcatatttaa cagactcatt ctttttcatg aatggtgatt
     6181 tgtttaccga cattaatctt gaagaaatgt tcagatttca taaagacaat aaagccgttg
     6241 ctactattgg tcttactcgc agaacagttg atattgattt tggagtaatt aaaattgatc
     6301 agactaatga atttactgaa tggaaagaaa aacccactct tgaatattta gtaagtatgg
     6361 ggatctatgt ttttgaacct agcgtgttaa aatatttgcc tgatggcttc tttaacgttc
     6421 ctgatttgat tataaaactg catgaggcaa aggaaagtgt aaagggatat caatataaag
     6481 gaaattggtt agacattggc cgcttggaag attaccaacg tgcctgccaa cagatggaag
     6541 caatagagaa aaaatcaaat ggctaatgct acaattgcaa taaccggtgc cggtgggttt
     6601 cttggtaaag cattaaccgc tttattaaaa aagaataatt ttgaagtagt tagtatttca
     6661 agatcctccg gtgttgatat tacaaaagca gaaacattaa ctacaattcc cgatttcgat
     6721 tgcctcatac accttgcagc tcatactttc gttccggatt catataataa tacagcagca
     6781 tttttctcca taaatttaaa tggaacactt aatgcacttg agttatgtaa gaaacgcaat
     6841 gcacgattta ttttttcagg ctcgtatgtt tatggtcaac caaactacct tcctgttgac
     6901 gagaatcacc ctataaacat gtggaatcct tatgcatcaa gtaaaattat ttgtgaacaa
     6961 ctgtgtcatg tttataataa ggagtttcag gttcctgttg acattttacg aatttttaat
     7021 atttatggtc cgggtcagaa ttcttctttt ttaattccta aaataattga aggtgttttt
     7081 aaaggccagt taaatcttga aacacttaca cctagacgag attttattta cgtaaatgat
     7141 gtttgtaatg ctttcttaca atgtttgtta attcattctg aagagcaaac atttaatgtt
     7201 tataatattg gtagtggcag tagctacagt gtacaacaaa tagttgacaa ggtgatttct
     7261 ttcactggtt taaatccaac gatatcgtgt aatgaaaaaa gacgtgccgt tgaagttgaa
     7321 gatgtaaaag cagataactc caaaattaag cgtgataaaa aatggcatcc gtccgtttca
     7381 tttgatcagg gacttaaaga tatgattgaa atttaccgga atgaaaatga tttataactt
     7441 attatcatta aactgaaatt ctttcagcaa caataaaagt acaagcatat ttattgtttt
     7501 catctggcac aacatctttt cttgatataa tttcccattg ctcccaatta atttgaggaa
     7561 aaaaaacatc tccattaaat tgatgatgta tcaaagtgac ataaagccta tttgcaagat
     7621 gtattgattg tttaaacact tgtccgccac caataataaa aagctccttc tcccctgcct
     7681 ttcttgctat ctcaatacct tctttaatac ttgaaaccaa aatagctccc ggaaattgaa
     7741 ttttagaatt cgatacaatt aaattcgttc tacctttcag cggtctgaat ttctcaggaa
     7801 tactctcata attttttcta cctgtaagaa cataatgacc taaagtagtt tctttgaaat
     7861 acttcatgtc cacaggcaaa tgccatggca aatcattttc agcaccaatt aaacgattct
     7921 gatccatagc agcaattatt gaaactaaca taaaattaaa ctgctacctg tgctttaatt
     7981 gcaggatgcg attgatagtc cgaaaaataa aaatcttcaa acttaaactt aaagacatct
     8041 tttaccaatg gattcatttg cagtgtcggc aattgatatg gtgttcgcga aagttggagt
     8101 tctacctgtt caaaatggtt ggaataaatg tgtgcatcgc caagggtatg aataaactca
     8161 ccaacttcca attgacaaac ctgtgcaatc atgtgtacaa gcaaagcata agaggctatg
     8221 ttaaagggca caccaagaaa aatatcagcg ctacgttgat aaagctgaca tgacaatttt
     8281 ttattagcta cataaaactg aaaaagtatg tgacatggtg gcaatgccat tttagataca
     8341 tcagcaacat tccatgcact cacaagcatc ctacgcgagt cgggattgtt tttaattgtg
     8401 tcaatcaacg aacttatctg atcaattcca ttaccatcag gagttgacca cgaacgccac
     8461 tgatgtccat atacaggacc gagattccca ttcttatcag cccactcatc ccatattgaa
     8521 acaccatttt gctttaagta ttcaatattc gtttcgcctt tcaagaacca taataattca
     8581 tgtatgattg atttggtgtg aagttttttt gttgtgataa gcggaaaacc cttctgcaaa
     8641 tcaaatcgca tctgataccc aaaaacactt ttagtacctg tacctgtgcg gtcagatttt
     8701 tccacgccat tggtcattac atgcctgagt aactcgtgat actgattcat tctattataa
     8761 acaattttaa ggcttcaaaa ttaaattaaa caaatggatt tcatgtctga ctatgcatcc
     8821 gtagaggttg tttttattca caatgttaca tttaatattt gctcaatttg tgagcaataa
     8881 agtttaatct tcgtaaaact aaacagccgc taaaatctgt aattgatttt aacggctgtt
     8941 atttatcaaa caatctaatt tttacataat cttatctcaa caaagtaatt gttccattga
     9001 aatcatgtcg ctttccacct ttattggtaa cactaagtgt ataaacatag gttccctgag
     9061 gtgctaaatc acctttgcca tttgagccat cccatggttt tgatggagaa tctgactgga
     9121 acagcatttg tccccaacgg ttgaaaatac gtaaattaaa tcctgaataa tccatcgcaa
     9181 aaccaggatc aaagaacact tcatttcgtc catcaccatt tggtgtaaat gcatttggta
     9241 accagaatgg attaatcaca atggtctgtt gtgtttcatc tttacaacca gaaatgtttt
     9301 caatcatcaa cttaacagga aactctccaa tctctgggaa ataatgggta acattctgat
     9361 catataatgt atctccttca ccaaacaccc atttccaact aactgcatag gcagactgat
     9421 cagtgaaact tactggtccg ttgaaaatat ttgtcaccat tggatttgct gtaaattgtg
     9481 caacaggcaa tggatagaaa attacttcta cttgtttctc tattgaatct ttacatccaa
     9541 atggattggt aacaatcaac tgcacattat aagtacccgg ctgactgtaa acatgtgaag
     9601 gattaacttc attggaaaca gcattttcac caagactcca ttggaatgtt gctccaggct
     9661 gatacaatga ttgattgaag tagttaactc ttggcacctg acatgttttg cttgtatcgt
     9721 ttgtaaaatt aacaacagga tatggatgag caataaatgc aaatggtgga gataattttt
     9781 tacaaccatt tatatcagta acttctaccg tatatgtacc tgtagtagta actgccgttg
     9841 tacttcctgt agttgcatta gaccattgat aagattggta aggaagtgtt gttgatagaa
     9901 cagaaggtgt accttcacaa acctgattta cacctgtaat gacaggctgc ggcaacggat
     9961 taactgtaat atttgctaaa ccacttattt gcgttccggg gcaattctta tcactaattg
    10021 ctgtcagatt atacgatgtg gtagttgttg gactaagatt tattgttaat ggattttgtg
    10081 atgttgttcc attgttaatc tgtcctgtca cgttattctg ataactataa ttaaatggag
    10141 ctgttcccgt aaactgaatc tgtattggtg ctatttcatt taaacatatt acagggttgc
    10201 ctgaaataac agcttgtggt aattcatgta ctgtaacagt tgttgctcct gttgcagaag
    10261 tcttacagcc taataaacct gtaactactc cggacaatgt atatgtagta gttgccgttg
    10321 gcgtaacagg aattgtcatt gaattacttg tggacgtcac ggggaagttt gatgtgccgt
    10381 cattgtaagt tacactataa ggcccatttg cagtaaagtt aagagtaata cttcccggat
    10441 ttcctttaca caatgaaggt gttgccgttg aaatagctac agttggatta tccagcactt
    10501 taacaccaat gttctgatta attactgcag ggcaattatt atctgtaatg tttgtcagta
    10561 cataagatgt attgctttgt ggcgaaacat agaatgtatg cggattagat gttgcgttag
    10621 taacaccttg tccctgtaac tgatatgtaa acggtgcagc acctgtaaat tgaattgata
    10681 attgtgtact atcaccttta cagattgaat cttttgcagt agaaattacc gcctgtggta
    10741 ttgcattgac tgtaatcatt gcattacctg aagccggccc gttacatcca tttccattaa
    10801 ctgttgccgg aagtgtataa tttgtattgg cagttggact cacaggaatt gttacagaag
    10861 tattattggt tgtaaatggt cccattgtag ttccaccggc caagtaagaa taattataag
    10921 gtgcttctcc ggtaaaattg atggtgagat ttgttgaacc tccactacaa attgcattgg
    10981 ttcccgttat tgctgctgtc ggtattggag taactttcat gtaaaccgac tgattgatat
    11041 tgctgttaga gcaattagca tcagaaatac ttgttgacaa tgcataagtt gtattaacat
    11101 ttggtgcaac aggaattgaa acagagtcaa caggcgaaga aaggctaccc gatgaaacgc
    11161 catttgttgt ataactatag tcgaatggcg gtgtacccac aaatttaatt gcaatgaaag
    11221 aactgcttcc tacacaaatg gtatcatttc caaataatgt tgcagttggt tttggattaa
    11281 ctttcacaac agatgaacca ttcaacgctg tgcctgtaca atattgatcg gcaatactaa
    11341 caggagtata ggttgtagtt ccaacaggcg gacttacggt tattgttaat ggatttgtac
    11401 ttgttgttcc atttacatta gatgttccat taaaataact ataattgaat ggtgctgtac
    11461 cggtaaatgt tatgttgaaa tttgctgatg tactctcaca tattattgga ttaccactga
    11521 tagtcattgt tggcaatggt ctcacagtaa ttaaagcagt tgccggatta acaggtgcat
    11581 tacaaccata catgctgtta acatttccaa ctaatgaata tgttgtgtta actgttggag
    11641 aaaccggaat aacaatagaa tttgtattgc ttacaacagg tgtgtttaca gttccatttg
    11701 agtatgctgt agtgaaaggc ccgttacctg taaagtttat ggtaatactt ccactcactc
    11761 ctgtacataa attaggcgtt gttgttgtca gaacagcagt tggtaaatta agaactgtaa
    11821 tttttacttg ttgacttgtt gcacttgtac aaagctgatc atttacctga accaatgtgt
    11881 aatttgtagt agttgttgga ttaacattga tggtaacagg gttggtagct gcattctgca
    11941 ctgcttgtcc ttgtaactga taactgaatg gtgcagtacc ggtaaactga attgttaagt
    12001 tagttgcatt accattacaa atagtatcgt tgttagttgt aaatgttgct gttggcaacg
    12061 gtcgcacagt aacactacca attccattta cacttccttt acaaactccg ctaaatacag
    12121 aatcaagttg aaatgtagta gtagctgttg gactaacaac taccttatat gggttattac
    12181 ttgttgtaac cggaccataa gtatttccta atccatcttt gtaatagaat gtccatggtg
    12241 cattacctga agtaaagttt actaataaag tatcattcgc tccggtacag attgttttgt
    12301 tgccgctgat agatgcctgc ggcaatgcgt taaccgttac aattgctgtt gcaggattgg
    12361 tgttattaga acattttgaa tcattaatgg tagcacttaa tgaatacgtt gttgttgcac
    12421 ttggcgaaac attaaatgtg taaggatttg ctgaagtatt aaaattggtg gtgccacctg
    12481 tacttgcaac atagcttcct gtaaatggac ctgttccggt aaagttaatt gtcaaagttg
    12541 ttggctgacc ggcacaaatt gttgtagttc cggaaagtac ggcagtaggc agttggttta
    12601 ctgttacagc tactgtatca ttgatatttg acttactgca aaatgcatca gtaactgttg
    12661 taactatata attagtactc gttgtaggat gcacataaaa ataataggga ttcgtagctg
    12721 catttacatt tgcaggtgca ccggcattgg tcaccaaacc aaatgtgtat ggcgttccat
    12781 tgcctgtaaa actaacctgc atgcgcacgc tgtcattttt acagattgtg gcattggtaa
    12841 cacttgaaat gtttgctgta ggaattgctt ttacagcaac cacaacacta ttagcaattg
    12901 taccggggca agccttagca gagctaacaa aattctggaa tgaataagta gttgttgcat
    12961 tcggagtcac attgagtgtc acaaaagatg ctgctgtatt taccggtgtt aaaacacctg
    13021 ttgtattatc tttaatattt ccaacaaaag ggcctgtgcc tgtaaagtca acacgaattg
    13081 tagttgagct gccgctacag atagtatcgt ttccggtaat agacatagca gcagttggta
    13141 ttggaagaac aacaattttt ctggttccgg aaacggttcc tgtacatgca tctttaacac
    13201 taaacaatgt ataggttgtt gtggaagcag gagtaacatt tattgaaaca gggtttgaag
    13261 cagttgttgc tgtaatatca ggacctgtac tgcttttaat aacataattc caaggaccag
    13321 tacctgtaaa tgcaagtgaa atagtggaac cacctccatt gcatacagtg tcatttgcag
    13381 tatttacaac acttgcagtt ggcaatgctt ttattgttgc atttgcactt ccacttactg
    13441 ttcctgcaca agcacccatt acagaattga gtgtatagtt aaaattacaa ttaacaggta
    13501 ctctgactgt aaccggtgat gaattggcta ctgcattaac aacagttgtt ccaacacaac
    13561 ccgggccggg tacattataa ctatatgtat aaggagcacc acctgcaaat gtaaactgaa
    13621 gattcacact atcaccgggg caaacactac ttacacctga aattgcaata tttgcagttg
    13681 gaccaggtgt taatgttaca acgtgtattg gtgttgtact tgtacatccg cttggacttt
    13741 gcgttataac tgctgaatag tttcctgctg cacaagctgt aataccagaa gtagttgctc
    13801 cattactcca taaatatgaa gtggtaaaac aagcaggact aggattggcc gaaacggtta
    13861 aatttacaca atttggcgga caaagatttg ttggacctat aggtgaaata ctagcagtga
    13921 aactattatc aatatattgc ttggtaacat tagattttga aacacaccgt gtaactgttg
    13981 gggttgattt tataattaaa gtatcatcaa tagtgacata atacttcacg ctgtcacgac
    14041 atactgtctt tgaaaatgga tcggtatatg ttgcatttgg ccaggtattc ggcactgttg
    14101 caatttggac tggtggagaa cctataggat gttctcgata aattttatag actaaagctg
    14161 cagaacccgg atatgcatgt ttaaaaagcg aattccaatt tacttgtgtt tcacaattca
    14221 caatacttgt actgacaaac attgtactaa ttgtatctga aggaacagaa atactatccc
    14281 cattacacaa tgattttgtt cttacataga aataaacagg tgcagcgttt gcgttggcac
    14341 caatcaatgt atttagttgt gtggcagtga tagtatcacc tttctgttta tagaaatcct
    14401 tatttcctgc aacagaatct actaaagtgt atgggccacc ataactattt gatgcataaa
    14461 tctgatagga attgaataca tgatgcgtat cgagttggtt gacgacaccc ggtgtttccc
    14521 aatataatcc aacatcaccg gttgtattaa gcaccgatgc accccgaaca tgaggtggct
    14581 ccaagggttt agattgaaca acagtaattg caatcgttat agtactgatt gccggtgccg
    14641 gacaataatt atcctgcgct ttaatgatga agtagtaggt atttctttct cgtaaacacc
    14701 ccgagggttt aatgatgtga tcgcatgttg tgacccattc aaaatttata aaggcagcta
    14761 aaggtgaaat tacaggggtc aatttatcta acttggcaca aggtgggtaa ccacatccac
    14821 tgtttgtatt tataggcaca ccaaactcat cgccataagc atctatacga attgtctgaa
    14881 atgcagctcc tgaaccaatg ttccgttgaa agtctgtagc atcaatcctg aatttgaccg
    14941 tatcaccaac atgaacagta gtatctttca tagttcctcc ggcaaaaggt aaattaattg
    15001 ccggtggcaa attaattgcc ggtggatttt gtacaaaaca attagtagta agcactacac
    15061 cataatctct gaaaatctct gcaactttct gttgacattt ataagcagta actttcaggc
    15121 aagttgcaaa attaccagtg acacttgatg ctgttgttac aaaaaaagtt acttcacctg
    15181 aattatgatt cattgttacg ttaccaggca actgactgtt aacagtatag ggtgcagtaa
    15241 aattaattat tggagcattg aaggcggcag gaggagaact tgttggttgc tctaatggat
    15301 tagtccaatc actataaagt gaatctaatt cgggatctac ggcagttgga ttgtaagtat
    15361 attgatatcc gatacagata tatgtattag gtctttcttt aaaaaatgga gaagaatcat
    15421 aacaaggatt catgttttgt ccattaaacg gatacataac agctctgatc gtaaaacctt
    15481 gtccacctcc accaacatta accaaagtat ttctgcaaca atcgtcaatt gcaaaaaccc
    15541 atcctgaagc aggaggagtt ccatttaaaa acaccggatt tgttttcatt acaaattctt
    15601 gagttgcacc agctgcagga tttgcacaag taaaaccaca acctgtggga cttaagtcag
    15661 ttattacagt taatgcagca ttaattggaa tggaagtaac caatggattt ccatatacat
    15721 taattgctga tgggggcgta aaaggaatac cattacaatc tctgtaaact tttaaggtaa
    15781 actgaaattt accaatattg ggccctgatt taatacattc ccaagtaatc tcaccaccaa
    15841 aaaggtgact tgctttgaca tctactgtaa acactgtaca gaacatcaaa agtgcaataa
    15901 tgtgttttcg tatcatagct tcattaaatt tgtttgtaca aatataaaac taatcttcat
    15961 ttctacatca taaatttaac aattaaacga gattttttta acagagttgc cgtttgaaaa
    16021 aattcattga acttaacctg cagaaacctc agtcatttta ttagaaaaat aacgatttgc
    16081 caagtcaagt aattggtttg aatcagtaga attcatgatt tgtaaatatg aatttagtaa
    16141 actgaaatcc tgattgtata aaaccataga ttttattcta tcagaaacaa gcattggccc
    16201 atctaaactt ctttgataaa cgcctgtcat atagcttttt acacgattga gctcattatc
    16261 cgggattttt tcttcacgaa gtcgcttaat ttcaagatat atctgttcaa gcgcgttttc
    16321 acgatgttgt ttgcccactt gtgtagaaat gaagaagtat ccgccatgaa gccaggacac
    16381 cattgcacaa ccaaccccat aggtataacc tcgttcttcc cttaaattac tcatcagcct
    16441 tgaaccaaaa tatccgccca aaacggttga taaaactgtc aatggaatat agtcgggatg
    16501 aagtttattc ggggtatgac atccaatccg aatagcagtt tgtaatgcat cctttttctc
    16561 aagatatttt ctttcaggct tatatttttc aatagaaaaa tttttctcaa ctattttact
    16621 tagtgtagag tctttccatg gtccaaataa ttgatttaag gatttaacag catcctcatt
    16681 aaaacgtccg cttagaaaaa tcacacaatt ttgaggttga taatattgtt tatgccataa
    16741 aaccaaatca gcagaattca acttcttata atctgcttct gatgctggcc tcccataagg
    16801 atgattatcc ggaaatagca tctgattgaa agtctttctt gaaatataat caaccttttg
    16861 actattgatt tttaattgct gaatactttg atctgtgaat attttcactt cttcatcagg
    16921 aaatacagcc tctgacataa tttccagtaa caatgaaaaa gtctccggaa taaatcgtga
    16981 taacgtaaaa agtttaattg atgctgtatc aaatgaacaa gaggtgttga gataggctcc
    17041 atattgatcg aatgactgtg aaatttcata ggctgtacgc tttgctgttc cggattccaa
    17101 caactcattt actgcagagg ccaacatact aaactcacca taccactccc ctgccataaa
    17161 aaccaattcg agttttgcaa cttcctgctc acctgcatta agaacgtatg cttgtatgcc
    17221 attgtccaag ataatttttt caggtaatgt aaaatcaact ttattcgtta atttcaattc
    17281 aggttcaatt actctattca tactgcaact cctttcttgc aatagtaaat tgatgactca
    17341 ttttcttttt taaaaattgt tttggctacc tgctgaacat tatttgcagt aacaacttcg
    17401 tattgttgta actgattatt tattttctct gcacttccta tcatctcaaa ataagcaaga
    17461 tttaatgccc tgttaccaat atttacttct gaaaaaacta aagtagattc tgttttttgt
    17521 tttactattt tcatttcttt ctcttgcact ccatcattca ctacaagttg caacaaagta
    17581 tcaatagcat gatcagcatg ctttacatca acaccatcat taacttttcc ttcaactatc
    17641 attaatccat tatccaagtc tcctgtaaaa taggcattga ttgaattaaa tagttgttgc
    17701 tctttaacta aagagtgata caacacggct gatttgcttc cacagagtat gtctgtaagt
    17761 aattcagtag cataaaactc gttatcaaat cgtgaacaag tatggaaagc tttataaact
    17821 gcatttgcag gaatgtctct ttcaagcata atatttctgt attctctctg tattggttct
    17881 tttggtaaac tctccttaga taattcatgt gcatttattt caccaaacca tttttctgct
    17941 agttcaaatg caagttcagg ttttatgttt cccgataatg taagtatagc gttattggga
    18001 caataccact tatgaaaaaa tgcttttaca tcatccaatg ttgcatcttc aatatgttcg
    18061 atgtctttac caattgttgc ccattgataa ggatgcacct tatatgcaag tggcctgaga
    18121 ttgagccaca catcaccata aggttgattc aggtagcgtt gtttaaattc ttcaattacc
    18181 acattacgtt gcacatctaa acttttttct gagaaggcaa gacttaacat acggtcactc
    18241 tctaaccaga atgctgtttc aacattttgt gaaggaatag tcagatagta gttagtaaaa
    18301 tcattatttg taaaagcatt attctcacct ccggcacgct gcaatggttc atcatacgat
    18361 ggaatattta cagaacctcc aaacattaaa tgttcaaaca aatgagcaaa accggttctg
    18421 cctgcatctt cattgcgtgc accaactttg tacaaaatat tcattgcaac caatggtgtt
    18481 gaaggatcgt aatttacaat cacaggtaaa ccattgggca aaattatttt ttcaaatttc
    18541 accataaatt aaaataaatt agcaatacgg tttttttcta ttaaaaattc attccaaata
    18601 tttttcacta tgtcaccggc aggaataata ttatttattt gtgacgatac ctgaccaatt
    18661 tctaattcgc cttcgtccaa atctccttca aacattcctt tttttgctct tgccctgccg
    18721 agtaactgcg tcaattcctc acgcgttgca cagcgctgtt gtgccagatc aacttgttca
    18781 ctgaatttgt tctttatcaa tcgcacaggt gtcagttgct ttaatgttaa aactgtttga
    18841 ccctctgaag tattaataac tttttcttta aagttagtat gtgctgatga ctctatactt
    18901 gccacaaaac gcgacccaac ctgcacacct tctgcaccta atgcaaaagc tgccatcata
    18961 gcacgaccac atccaattcc acctgcagca atcaccggta tttgtaaagc atcctttacc
    19021 tcaggaatca aacacaatgt tgttgtctct tcacgaccat tatgccctcc ggcttcaaaa
    19081 ccttcggcaa caactgcatc aacaccggca gcttgcgact ttaatgcaaa ttttacatta
    19141 cttacaacat gtacaacagt aatccctgct tgttttaaat gagtagtcca tgttgcagga
    19201 ttaccagcag aagtgaaaac aattttcact ccacctgcaa tgataatagc catcagtttc
    19261 tctatatcag gatacaacaa aggaacattt acaccatagg gtttatttgt ggcagcctta
    19321 catttatcaa tatgctcctt taaaatatca ggatacatac tgcctgcacc aattaatcca
    19381 agcccacctg cattgctaac tgcagaagcc aatctccatc cactgcacca aatcattcct
    19441 gcttgtacaa ttggatattc tattccaaac aattcagtaa ttctgttttt cattctttct
    19501 gttctgatgc aactatacaa aatattttta ctgataatct gtctgtattt ttaaagttat
    19561 tcaagttgca tcttaaaaac taaaaagcat accctattcc tgtttcaaga aaatcggctt
    19621 gaccaagatt ggtcttaata taaacaccgg cataaaaatt attcttgtga tatatggtag
    19681 catctttcag ataatattga aaacctattc ttgcagtgat ccaacccttt aattgttttt
    19741 tgaaatctgt ttcgttttct ctccttaact tttcatgata aaccggatta tagaaatata
    19801 taccaccgtt aatcagcaaa ctaaaattac cgaataaaaa ttcattacca acaactgcct
    19861 gcaatgctgt tgaacgcaaa tgctctttat ccttataata acgctggctc gtaataaaat
    19921 cataaacgcc actgttgtac cacccttcta cacccaaatg caatttattt accggagtca
    19981 gataacgagt cacataaaat gctccgagat aaattggata tcttggacca tttaccggaa
    20041 atgtgcttgc cccttgctcg ttgtaaccaa ctgctaatcg cacctgaaaa tgatagttgg
    20101 catccttttc taaactacga ttaccaaaga gtagattaag tggtcggata tgataacgaa
    20161 gtcctgcaga tgcaaccggt aaattaattc caagattagg caattgataa tgtgaattgg
    20221 agcaatgata aacaccggca cctgcactga cagaccaaaa tggattgata taatatcgta
    20281 ggttgagtcg tgctgctgca caaaatgtaa atgctgaacc tatgagtgtg ttttcaggat
    20341 tattgatggc atgatatggc ttattaaaat atgcgaaacc aaatgatggt gtaatttcag
    20401 tataaaattt atcattcatc ttataactga attctaacct gtaaagcaat gcagagaaat
    20461 taccaagaac ttttttatta cctaaaccac cataaacaag ttcgaaagat gcattaggga
    20521 agttataata aggatgccat gccttatagc catttaatct gctgccaatc acaatactgt
    20581 tgatacctgc caattttctt tcaggaaata taggataatt tttcactact ctacctgcat
    20641 taaaactata ttcaacaaat gcaggttgcc ttaatgattg tgcatcagaa aacaaaccac
    20701 aaaacaagaa agcaaaaagt aaaacacaat tgaatgaatt aatcatccag ttaaaatgat
    20761 tttataatgg aaataaaact tcttgatttt agagatgctc ctccaattaa cccaccatca
    20821 acatccggca aagcaaacag ctcctttgca ttgttttcat tacaactacc tccatacaaa
    20881 attgaagttt cagaggcttg ctctccaaat tgtcttttta aaacttctct gataaaataa
    20941 tgcatttcct gtgcctgtgc agacgaagct gtcagtcccg taccaattgc ccaaacaggt
    21001 tcgtaagcga ctatacactt cgcaaattcg tcagacgtta gatttgaaag tacatttttt
    21061 atttggtttt ccacacattc aaagtgattg ttgctttctc tctcggtttt tgtttcacca
    21121 atacagaata ttacctgaag tccattcaat aatgcctgct tcactttcaa caaaagtact
    21181 tcatcggtat cattaaaata ggttcttctt tcactatgtc cgagaataac atagccaaca
    21241 ccgcatgagg caatcattga agctgagact tctcctgtaa aagcaccgga agcctgtgct
    21301 gcacaattct gtgcagcaat tttaattgca gtactattag tgagtttact tacgttattt
    21361 aaaaacggaa atggtggtga taatacaacc tgaacatcag aattaatttc gtccttatac
    21421 atttctacaa tttcagaagt cagtttgatg gcatcatcca atgtgccatt cattttccag
    21481 ttacctgcta ctatttttct acgcatattt tttctgctaa aataaaattc tttttaatct
    21541 ccacctccct gtaatgaatc tgattttaaa gagtcacttt caagaatcat caattctgta
    21601 tttatcaacg aatcatttaa taaagaatct gccgcttcga gctcaatgtc atcgttttcg
    21661 caataaattg ttttggaaat tttcttcggt tttttgaatt ttccaacaac aacacccgac
    21721 ttgggatcct gataagcttt ctctaacatt tttccgaata ttggaagtgc taatcgtgac
    21781 ccctgacctg atgaatttcg aaaatgcacg cttctatatg gagcacctac ccaaacaccg
    21841 gtaaccaaat catgagtaac acccatatac caagcatcgg aataattgga tgtagttcct
    21901 gttttgcctg caacatcatt tccattttta aaaactgaat agccccacaa tgcctgagat
    21961 gttccaccag gctcctgaat agttccttgc aacatataca acattaacca ggcattttct
    22021 tcagagataa cttgcttaaa ttctggcata aactctttga gaactgttcc atcgttggca
    22081 caaattttct tcaccaaaat gggttcaaca ctttttccgc catttacaaa tggtgcataa
    22141 gcacaagtca tttcgtaaac acttacatca cttgttccta aacaaattga tggcacagta
    22201 tctaaacgac tttttacacc tgccctatgt gcacactcaa caattttgtc ccatccaacc
    22261 aattcagcaa tttgtgctgt aatagaattg accgattgtg ccaacgcccg tcttaatgtt
    22321 ttacttgaat aagtaaatgc acctgtacta ttatagggtt gccagatttg accaccatca
    22381 tactctatgg atacaggcac atcatctaat cggctacaag ggctaaaagt atcttctatt
    22441 gctgccaaat aagcaaacgg cttaaatgtt gaacccggct gtcgttttga ttgtgtaaca
    22501 tgatcgaact taaaaaaatt aaaatcaaca ccacctacat aagctttaat ctcaccggtt
    22561 aacggattca aagacatagt tcctgcattt aacaaacgtg cataatattt aagtgaatct
    22621 aatgttgaca tcacggtatc cttggcacca ttgtatgtaa atactttcat gctttttttc
    22681 ttgttaagat aaaaattaac agaatctggt gctgaaggga attgcttttt caaacggtcg
    22741 tatgactgcg taaatgccac agcggtattt gcgaagttca atatttctct tccattgtca
    22801 tcgcgccatg gattttgttt tccccaatgt tcatcgaatt gcttctgcac ttttctcaga
    22861 tgatctttaa cagccaactc tgcataattt tgtaggtgtg agttaatggt tgtataaatt
    22921 ttcaatccat cctcattaat gtttaagttg ttttcatcac aataactttt aataatcttt
    22981 gctattaact gcctcaggta agaatctttt gttgcagggt tattggttgg acgtttaagg
    23041 ttcaatggca ttggactgat ggaatcaaaa gctgcctgac tgatataccc atacttctgc
    23101 atctgcgaca acacaatatt tcttcttgcc aatgagcgtt tcttattatt caaaggatta
    23161 taactacttg tagctttgag caaaccaatg agtgttgctg attcctgcaa atttagttct
    23221 gaaggttgtt tgctgaaata gttttgtgca gcaaccttga taccaaacca attattacca
    23281 aaatcaactg tattaaaata catttccaat atctcatcct ttgaataaaa catctctaat
    23341 ttaacagatg ttatccactc ttttgatttg ctaaccaatg taccaacaag aggaatatgt
    23401 tgaagtaatc cctgattaac tttcttgcgg gtactgtaca tatttttggc tagctgttgt
    23461 gttattgtgc tagcaccacg ctcatctcct ttggcagtgg aatatgcacc actgattaat
    23521 gcataaaggt caaggccgaa gtgtttatag aaccttacat cttctgtggc aacaagtgcc
    23581 tgtactgcat aagtcgaaat tgaatcaaaa cttacaggtg tcctgttttc atgataatat
    23641 tttccaataa gtacactatc ttctgtataa atttcggtgg caattgccac ttgcggattt
    23701 ctgacttcac tcatttttgg catatagcca aatagccaaa gaaagtttat atgcagtagc
    23761 acaacataat aaaccatcag cataaacaaa atagctgcac ttcttaaaaa cttgttcttt
    23821 attattttga gataggaaaa cgttataacc gattttaaaa tcagaaatag ctgtttataa
    23881 attctttgaa aagctgaaac accctcaggc atattttgtg gcattaagtc aaacacatta
    23941 acaagttgca aagaaataaa cctttctgtt taatttcatc ttttaggcat acatttgtca
    24001 gcagtttttt aactaaacat aaatgagctt aacagaacag tttaaagaag tcgtaaataa
    24061 agaatatcaa tttaaaggcg acagcatcgt actcggtgca ccggtactta aaggcgaaac
    24121 gctgacagga agtttcataa aagcaccatt aaaaactttt aaccgtcatg gtttaattgc
    24181 aggagctaca ggaacgggaa aaaccaaaac catgcagggc atagcagaat cgctttctgc
    24241 gaaaggtgtt tcatgtttat tgatggatgt aaaaggcgat ttaagtggat tggcaagtcc
    24301 gggaacaaca aatccaaaaa ttgatgaacg ctatcagaaa ttaggactta cctggaagcc
    24361 actgcaattt ccggttgagt ttctgacttt atcagatgaa aaaggtgtgc gattaagagc
    24421 tactgtgtct gaatttgggc ctgtactttt ttctaaaatc cttgatttga atgacacaca
    24481 acaaggtgtt gtttcgctga tatttaaata ttgcgatgat aaacaaatgc ctttacttga
    24541 tttgaaagat ttcaaaaagg tattacagtt tttaactaat gaaggtaagg atgaaattaa
    24601 agcagattat ggtgctataa gtagtgcttc ggcatcaacc atcattcgta aagtaataga
    24661 gatagaacag caaggtgcag agaaattctt cggtgaaaaa tcttttgaag tcgaagactt
    24721 gttaggaata gacgaaaatg gcaatggcaa attgaatatc atcaggttga ccgatattca
    24781 ggataagcct aaattatttt caacttttat gttgagttta ttggctgaag tatattcaac
    24841 atttcctgaa gtgggtgacc cggatgcacc taaattggta atatttattg acgaagcgca
    24901 tttaattttt aacaatgcat ctaaagtgtt gttagatcag attgaaacag taataaaatt
    24961 gattagatca aaaggcattg gagtattttt tgttacacaa tcaccggaag atataccggc
    25021 tgccgtattg gcgcagttag gattaaaagt acaacatgct ttcagagtat ttacagcaaa
    25081 cgacagaaaa gcattaaaac ttatagcaga aaattatccg ctatcagact actataaagt
    25141 tgatgaatta cttactactt taggtattgg tgaagcagca atcacagttt taaatgaaaa
    25201 aggtgtgcca acaccaatag ctcatacttt gctttgtaca ccggcatcac gaatggacat
    25261 tttaactccg gatgaattga caaaaagtat ttctaaatct cgtctgacaa caaaatacaa
    25321 tgaaacactt gacagagaaa gtgcatttga agtactctct aaaaaattag aaacgcaggt
    25381 tgctgaaaat acaaaagcaa caaaagcagc tgaagcaaaa gaagaaccca gccagttgga
    25441 tcaggtgcta aacagttcta caggaaagca agtgatgaga actgtaacaa gtgtactaac
    25501 aagaagctta cttggagcat taggcttagg tgcaacaaga cgcaaaaagt cgggttggct
    25561 ctgatattgc atttattaag ttatgacatc acctaagtca gagtggaagt atgatttaat
    25621 atcaaccata tttccaagta agtttttaaa gcgcgaaata cgaattgatt attatttgcc
    25681 tgcacaatac tctaaaaaca ccggagtatt gtttatgctt gatggtcagg atgcagaatt
    25741 gttacaccta aaagatacac tgcagagttt gacacagtta aaaaaaatta aacctatcat
    25801 agtaattgct attcatgcaa ataaaaacag attgcaggaa tatggtgttg taggtaaggc
    25861 tgactatcta aagcgtggaa ataaagcaaa acaattttcg aaatttattc ttgacgaact
    25921 gattgcgcat tgcagcagtt ttacaggaat aaaatttaca ccatccaaaa ctgcaatagc
    25981 cggtttttca ttgggtggat taacagcttt tgatttggca tggaaccatc cttctgtttt
    26041 tgaaactgta ggtgttttta gcggcagttt ctggtggaga aaaaaatctt acgaagacgg
    26101 atatactgat gatgacagaa ttatgcatca ggtggtaaag aaaagtaaat cggcaccggc
    26161 aataaaatgc tggattcaaa caggcactaa agatgaaact gcagacagga ataaaaatgg
    26221 tgttattgac tctattgatg atgcccgtga tctgattttt gaaatgaaac aaaaaggtgt
    26281 tcctgaaaaa aatattcaat atgtagaaat tcccgaaggg cagcataatc agataacatg
    26341 gtctgcagcc ctacccttat ttttgtgttg ggcatttggc aacaaaagaa aagttgatat
    26401 ttattgatta tactttttga tgtgaggtgt ttaagctttc agcaaaagat tttagattta
    26461 atccgtcaaa agtacctgag ctcatcatta aataaactac ttgttgttta tcttctgtgt
    26521 tcagaaaatt tattaattgc attgagtcat taataacttt tatgtcgctt ctgtcaaatg
    26581 attggaaaat tttttcggaa ctaatgggag gcaatttttt atgttcaata gtttttggat
    26641 taaaataaac tactgcaaca tctgcatcag ccattgtgcc tttatattca tcaagaaaat
    26701 tttcatttaa actgctaaac gtatgtaact ccatacaggc aataagtttt cgttccggga
    26761 attgcctgcg taccgcttct aatgttgctt taagttttga aggtgaatgt gcaaaatctc
    26821 taaaaacggc aaactgatca ttctcttcaa gtaattcaag ccgtctggct gctcctttaa
    26881 atgattttat tgcattataa aaaacttcgt tattgacacc tattgagttg catatcatcc
    26941 ttgccccttc tagattcatc agattatgct caccaaaaat ctgaagcggc acttccttat
    27001 tatagtcatc gtaaataaat gtttttccat ttctaataac atgtttcgga atggaataac
    27061 cttttctatc agctacaaca ttttcagcct cacaaatttt acgaacagca tcatcattca
    27121 tgcagtaaaa caattttcca tcacgtggaa tttctcttgc aaaaatcctg aattgctcct
    27181 cataaatctc cagagttgga aacacattta tatgatccca tgcaatgcca ctaatgagac
    27241 caatttgtgc atgatacaga tgaaacttag gtcgtctgtc aatcggagaa gacaaatatt
    27301 catctccctc cagaacaatt ataggggcat cgtttgatag tttaaccatg gtatcaaaac
    27361 cttcaagttg tgcacctacc ataaaatcaa agttatttcc tgatgttttt aaaacatgta
    27421 gaatcatgga tgtaatggtt gtcttgccat ggctgccacc tattacaact ctggttttat
    27481 tttttgaatg ttcatacaaa aattcaggat aagaaaacac tttaatattt ttttgaatag
    27541 cctctgcaag ttcagggtta tcggcacgag catgcatgcc caatataacg gcatcaatat
    27601 cagcattaat tttttcagga aaccaacctg ttttctcagg taatattccc gatttagcca
    27661 atcttgtctt tgcaggctca acaatttcat catcactgcc cgacacttca tgaccaagtt
    27721 gatgtaaggc aagtgccaga ttatgcatag cacttcctgc aatagcaata aaatgatatc
    27781 tcatttccga tttttgacgc taaaatacgc tgtcataatg gggtattttt aaagcttcaa
    27841 actttttatt aacgatattc atccctgttc ttgttattaa agataagtga tttaatttag
    27901 ttttgcgcaa aaaagtttgc attgaaggca aaaatcttag acctctacat tataaaaaag
    27961 tttttaggca cattcctgtt tgccatagga ctaattattt gtattgccgt tgtgtttgat
    28021 atttctgaaa aagtagatga ttttattgag agacatgctc cattaaaggc tattgcattc
    28081 gattattacc taaacttcat cccctatttt gccaatttat tcagtccatt atttgttttt
    28141 atttcagtaa tatttttcac ctctaaaatg gccgggcaga cagaaattat tgccatttta
    28201 aacagtggca tcagtttcaa ccgctttctc cgaccttata ttatttctgc aacagtaatt
    28261 gctattgcat ctttttattt aaacaactgg gtgattccac atgcaaacct tgtacggctt
    28321 caattcgaaa atatttatat taaaaatcct tatgtctatc gcggaagaaa tattcacaga
    28381 caaataaaac ccggtgagtt tgtttatttt gaaagctacg ataacaaagc caacacaggc
    28441 tttatgttca gcattgaaaa atttgaagga ggaaaattag taactaaact catggctgat
    28501 agaattacat gggattcatt aacgcaaaag tggagcattg aaaattatac catcagacat
    28561 attaatggta tgacagaaga attaaagaaa ggcatcaggc tagatactac attatcaata
    28621 cacccatctg aatttaacag aagaatgaac tttattgagg caatggattt ttcggagtta
    28681 aacagctata ttaaagaaga aaaatcaaga ggctctgaag tgattccttt ttacgaagta
    28741 gaaaaataca ggcgcacctc ctatcctttt gcaactttca ttcttacact tattggcgtt
    28801 gccatttcaa gtaaaaaagt acgtggcggt ataggtttac aattaggggc aggaatatta
    28861 ttgagtttca cttacattat gtttatgcag gtgagcacaa catttgctac aaatggaaat
    28921 ctgccggcag ttgtcgctgt gtggatcccc aatttagtat atgcatttat tgccatttac
    28981 ttattacgaa aagcacctaa ataaaaagag gctgtcatta atctgacagc ctcttctatt
    29041 aaacggatca aattttattt caccgtgtca gctggataat cagccggatt aaatgctgtt
    29101 tccgggtggt tcatcctata acgataccct ataatcggca ccgctattga ttttaatggt
    29161 ttgtttgaag gcatcaacct gtaataggca attttgtttt ctaaaggagc tttatctacg
    29221 aatgcataag cacgcaacac ttcagaattt ccattagctt ttatagctcc tattttttga
    29281 tattcaatag catctacaga gcgctcaaca acaaaggaat ctatatctgt ttcgcgagag
    29341 gtgtaccaac taaccattac atgatgatcg cgagcctctg cttggaaaga aagtaatgtg
    29401 attggcagtt gttgtacatg cgttgtgcta cgaataataa aattatcaag aatagtttta
    29461 tctgtaccgc caccgttcat attctttgca ataacaatat tgtcagaatt tttccagaac
    29521 attgatcgct cttttggccc ctgataattc caaaccacta catcatcaac cataatttca
    29581 cctttacctg tatttggatt atagacaaag cggtaatttc tgaaatcatt atcttttacc
    29641 aactcataat ttgttgtttc attaacagaa tagcttcttc ctttttcgag atttacacga
    29701 taggcaataa tcaattttcc gttcttcatt ccaaagttga aataacgacc acgacttata
    29761 aaatcgcaat tatcatctag tcttttataa tcaaagctta tatcaattcc atcagtatta
    29821 aaataattat ctgttggaat agtcaaatca attccatctt tacctttacc tgcgctcaat
    29881 ccgtttgtgc cttcaacacc ttctgacatt actacagcaa aagggcttac ttttattgca
    29941 tcaggacctt tacttgcctt tgtaggatcg tcaccattcc aatcaaatgc acaaataact
    30001 tctccgttat ttacatcata cccctgaatc atattggtga gtgctccagc acgtgaatct
    30061 gtcacattgg aagtattcat aacgacagta agatatactg ccaatcccat tatagatgtt
    30121 ccggcagtaa caaaaattgc cttatacatt atagacatct tttgtcgctg acggagtttt
    30181 aattttcttg ccataataat tattttatag aatgaacctt ttgattatac tcttagagat
    30241 taaaaaaggt tgtaaaaact taaatcgaaa tttgtgattt cttacatttc attactgccg
    30301 cattactttc ttggtaatgg tttgcttaaa tccatttatc tgtataaaat agacacctgt
    30361 gggcaaacta ctcagttcac ttagtgctaa caagttcttt ccaggagaaa catcaaacat
    30421 ttgttttcta agttcacgcc catcagagcc tacaatagtc agataaatta ccccaattgt
    30481 tggagcatta atgtttactt taagaaaatc attgaatggt accggtgaaa tctgctctac
    30541 actaaaattt ccctgagaat tattgttgcc attagtatta tcgggcatct tcgctcctat
    30601 gccggtgcaa agtgcaaata ctgcgtttgc atttatattt gttgcctgaa cagaaactgg
    30661 attttgttca tcacctactt tactgaatgg tgtcacttcc cattgtccaa tattttttat
    30721 cagaactaat gtatcaccat ttttgaagcc aggaatttca ttcttaaaga actttaaagt
    30781 aatatttgct tttacttgtg aatcgaaacc aataattcgt cttgctaaag attcatcggt
    30841 tacatcctta ccatcaacaa gaaacggttg tatgccatca ggtaaatttt cagaaaccgg
    30901 atttccagta tagttataca ttgaaatatc gccagaaaca atcggttgca ctacaacatt
    30961 tgaaacaact ttaccatcac ttgagaaagg aattgtataa ctacttccac ctttcatatt
    31021 tttccaaata agtttatctg attctgaagc acaaatcaac gagccgcctg atgctgataa
    31081 tgcattagtg ttttcattct ctataatcaa agttttttga ttgagcttaa ttgttgagct
    31141 gcctaattct atacgattgc gaacttcaac attagttgcc agaatgccat ttccactcac
    31201 ccttaaaaca ccatacttaa actgttcttt attaataaca ccggagcctg aaccggttat
    31261 taattggtta ctccctacat aatcaactat actatttctg tcaagaaaat aattcaggtt
    31321 accattactg caaactgcgg cagtgcccaa cgtgttataa aagccttttg aattagctgt
    31381 tcttaccaaa cctcccgcat caacagtaaa acctgaattc tgatatttca ggttcgactt
    31441 aatttgtgta cttagtaaat ttaatattgc acccgattca actcttaaaa agtcaacagc
    31501 aggctgacta tgactactaa gtagtagatt cccgtctgtt actttaagtc tgcaatttac
    31561 tgtaatacgt tgtttaatct gatcaattac agacgctgtg cttctctggt aatttaggtc
    31621 tccatttgta caatatatca aatttccaaa aacagaccca atgcctgttc ctgctcttgt
    31681 tatcaatgaa ctgcctttgg ctataaattc attcccctga ataaaaagta cagacttacc
    31741 gccttcagga ttatcatcaa agtttataat gccattccac tgacggaatg aacttttaaa
    31801 gtaagctttt acagaatcat ttgatgcagt taatgtattc gagtgcaaat aaaatacacc
    31861 accggtctgc gaatatgcac cgttcacagt tagttctgac ttgccttcat gaccctttaa
    31921 gataaaagtg cctgattgta atgagaaatt cttatttaaa ctaatttgtg aaactccagg
    31981 tgtttttgaa aaacaggtgt ttccaccatc aatgttaaca tcaccattta cagataaaat
    32041 aacagcatgg ctgcccatgt tgaaattact gttaagccct gatatattaa catccccttt
    32101 aattctgttt tcttctgctc caacacttcc acttgaagta aattctgctg cagcatttcc
    32161 tgaaagaatc aggttgccat caacctttaa caatgagcgc gacttgctat actgacctgc
    32221 taatgttgta agtccattta ctctgaattt atctgatgca tttgaaaagt taagtaccat
    32281 atcatttgta acagtgacag aatttaatgc accagttgca ttacaagcat aataagcaaa
    32341 caacaaccca cctgtcattt caagagaatt aaatttaaac tctgtatttc ctgctgcagt
    32401 tgaagacaaa ttaaaaacac ctcttaagtc accatgcgtt tgcaggtact tgccgccaac
    32461 atttagtttt gcattaccat tagctgtatt taaagttcct ttattgtaga ttaattctac
    32521 atttcccata tttagaaagc caccgtttac attcagtgta acattgcctt caccattgag
    32581 aagaccagtt atttcaccac caatatttgc aatactatct gtagttactt tgaaagatgc
    32641 tggatggcta ccgctatgaa actcaagtac agaatttaca ttgggtaaaa taatcttacc
    32701 aattttagta tcagaaatac tacctgattt atccaataca acccagccgt tgttaatagt
    32761 taatgtgcct ttaatctgat gactttcgaa ttgattattc tgattccatt caaacaaggt
    32821 acttcccacc tgtgtgctta tagtgacatt tcctaaatta ccagtaatca cagacgccaa
    32881 tggtacagtt gcataagaat accatttctt gatttctaag gttgatgttt cattaaaggt
    32941 ttcaacagaa tttgttagta gcgatgctcc tgttaaagtg ttatcacccg gattccaaat
    33001 caactttcct gtaacaccaa tttgtaatgc tgcattatta agaagttgct taacattacc
    33061 accattcata tcaaatgtac ctgcaatatt gacagctcca tttatctgaa gtaatttaga
    33121 tggtgacaaa ctaagaacag cacctccaga aacaacaagt gttttacaga atgtttgttg
    33181 tgatattgtt atattatgtg cagatgaaat aacaacatca tcagaagaag ccggaacacc
    33241 ggtaggtgac caaatagcag gattactcca gctaccggaa gctgttgtag tttttgtaac
    33301 agcaaatact gcattgcaat aagcaaaaaa ataaatcaat gaaaagacaa gaaattgtat
    33361 tctaattcgt gacatgctca tccctcatct ttcttactct tataccatta atagtttcct
    33421 ctctgaaatt ttgtttcaaa tcagggatat taaaatccac tggtctttgc tctgcatctg
    33481 atgttgtagt acttgccttt gattttttgg agttggagat attaaaataa attattccgc
    33541 caacaacaac agccatagta actgttgcag taacagcaat taaacgataa gacagtcgta
    33601 gcttagcgct ttgtcttaac ctgttgggca ggtgtttctt tcttttcttc ctcatctgcg
    33661 taaatcctta tatgtaaaat cgctaaaaag gttgcataaa aaaagccgca ttacggtgcg
    33721 gccttataat atcactcagt actaatttat aaatttacaa acattaaatc atctacagaa
    33781 atagtagcat tatctgcaag aatggcggct ctttccatca cggctttaag ctcacgaata
    33841 tttccgggcc actcataatc aagcataaat ttagctgctt cgtttgagag ttgtgccaca
    33901 ggtgttttat ttgcctgagc atacaggtca aggaaatgtt ttgccaaaag aataatgtca
    33961 tcacctcttt ctttcaatgg aggcaaatga atcagaaatc cctgaatcct gtagaataaa
    34021 tcttctctga atcttccttc tttaacttca acagccagat ttctgtttgt ggctgccacc
    34081 attctgaaat cgagcttaat tgttttatta cttcctatac gctgaatttc tttttcctgc
    34141 aatacacgaa gcaatttagc ttgtaagctt aaaggcatct ctcctatctc atcaagaaaa
    34201 atagttcctt cattagcttc ttcaaacttt cctattctac gttcgcgggc atctgtaaat
    34261 gcaccctttt catgaccgaa aagttcactt tcaataagtt cctcaggaat tgcactcatg
    34321 ttaacggtaa caaaattttt gcgtgcacga ggcgagttat agtgtagcgc acgtgcaaca
    34381 agttctttac ctgttccgct ttgtccggta acaagcacca tcatattgct cttttcaact
    34441 ttctggatca gacgcaaaat tcttaacaca gcattactgc tacccattat attgctgtat
    34501 ttatgacggt caataatctg gtcttttagc atttcattct ccttcttcaa caccacattc
    34561 atgctcagat tttttactga gttttcaaga ttcacgaaca aattatcatt cttgataatg
    34621 tagtcctgtg ctccgctttt ataagaatca acaaccactt caaggctgtc ttgtcctgaa
    34681 acaagtatgc acatgatgga tgcattataa tctttaattc ttcgcatcag acctacaccg
    34741 ttcattcccg gcaagtgata gtcaatagtt acaatgtcag ggttaagatg cagattattt
    34801 aaacattcct ctgccgattc aaaatgctga atctcgaaat ctggattttt tgacaatgac
    34861 tggcgtatca gtgttgccac cattgcatca tcttccacaa taaaaatttt agccatagtt
    34921 ttattattta tacttctaca ttttttagtt cactctcaag ttcaatacag gcaacgctac
    34981 attcatcaga aatcttttta atcattgccg gataatcctc taaatttgac ttttctctgg
    35041 cgtacttttc tagtgttgca actgtttcgt acgtatcctt taaacctaca taattcaagg
    35101 aaggtttcat tttatgtgct gactgcctga ttttttcaaa gtccttatgt ttcaaacctt
    35161 cttcaagttc atcaatggac accggtatgt tagtcaaaaa caatgacaga aactctttaa
    35221 taaaatctgc attgccaccc gacatttcat tcagaaaact aagacttgtc agttttgttg
    35281 tggcctgaga actactttga ctattggcag cttcttcaat aggctgctct gtaacagact
    35341 cattagcctt gccgctaagt tcaataattt tattctttac ttcagtgggg tcaaatggtt
    35401 tcgatacata atcattcatt cctgctgcaa tgcatttttc tttttcgctt gcagaaacgt
    35461 gtgctgtcat ggctaaaatt gcaatctgcg acttatgacc tttcatttct gttctgattc
    35521 tttctgtagc tgcataacca tttaattcag gcatctctat atccatcaga ataacatcat
    35581 agtcattggc agacaatttc tctattgctt ccaaaccatt acaagcttca tcgtgatcaa
    35641 aaccccaatc tttaatgtgt ttatttatga ttaaacgatt gatgtcatta tcttctacaa
    35701 ccaggatttt tatctttcca ttaaaaccgg catgtacttc agcaaccttc ttcgcttctg
    35761 tttccacttc tccgggtgca ggtaagtcaa atggcatttc gaaaatgaat gttgtgccat
    35821 gattcaactt actttcaact cgtatgcttc catcctgcag ttctacaatc ttttttgcaa
    35881 tacttaaacc caatcctgtt ccgccatact tacgtgtaat gtctgttcca gcctgcgaga
    35941 atgaatcaaa gattgtagca agtttatcct cggcaatgcc aataccggag tctataactt
    36001 taaatcttat gctagccttt gtgtcgctga tattaatgag ttccgtttta agtgttacag
    36061 atcctttatg tgtaaacttt acggcattac ccataaggtt attaataacc tgactcagtc
    36121 taactggatc gccctttaac aactttggta actgctgatc aaaatctgct attatcttaa
    36181 cgcttttatc tgtcacacga gctttaaaca attcattgat ggtattaata agatcaaaca
    36241 aattgaaaca gaccgattcg aatgtcattt tctcagcttc aattttcgag aagtcgagaa
    36301 tgtcgttgat aattactaaa agattatctg ctgattttct tattgcattt aaataatgcg
    36361 attggtcttt atcaagctgt gattgcagca caatttttgt gataccaata attccgtgaa
    36421 ttggagtacg aatttcatgg ctcatatttg ccagaaactg ctctttcatt ttctgtgctt
    36481 cactcgataa aataatttgc tgtcgcaatt catcttcctg ttccttatga cctgtaatgt
    36541 cagtagcaat acccatgatt ccagtaagag caccttcgct attaactgag ggcatgtaat
    36601 gcacttcaaa aaatcggcct tcgaactcca tgttctgctt aacctcttct ccaagcaatg
    36661 ctttcttaac gtccttcagt cttacaggta gtctattcca gatttcaaaa acagactttc
    36721 caattacatc ttcttctgac aaacggattg tgtccattgc cataccttta aacaaggtaa
    36781 atatgcctga ctcattgatg ctgaacagaa ttaatggcga ctttgaaacc actgcattga
    36841 gagttctctc tgtttgtaca agtgctgttt caatattgct atgattgttt ttagatgctt
    36901 ttgattttaa tgcacctttt aaggaacgtg ctattgtttc cggtgttagt acagctttag
    36961 gaatgtaatc gcaggcaccg gccttcatca gttcaacaac tattttttct tcgccttgtg
    37021 atgatacaac aatgattggc gctgtgccac cacgttgaaa atattctgaa atgaaatcac
    37081 ccgcattcat cacagtcaga tagtagtcaa caattaaaac atcaaattcc atactcaatg
    37141 ctttctccat accttcatcg taggtatcgc caaaatgcaa atcggcatta aatcctgatg
    37201 actttattgc tctcttaatt gcaattctat ctacttcatc atcatcaaca actaacaggt
    37261 ttatcttttt gtaagacata ataaaaaatt atttaagcgg gtaacacact aaggttccaa
    37321 taagtattca atacagctat agcttcaacc aatttgtcga aagacacggg tttgataata
    37381 taacctgcta cgttgttttt atatgcttcc aatctgtcct gatcgtcttt ggatgttgta
    37441 agcataaaaa cactaatggt ttgaagttct tcgtcatgtc tgatttcacg aagcaactca
    37501 agaccattca tacgcggcat attaatatcg agcaacataa ttttaggcaa ggggtcaagt
    37561 ttttctttac cattcaagcc cttaagcatt tctaaggctt cttcgccatc acgagcaatg
    37621 tgcaatgggt ttttaatatt attcttggca aatgcacgag taacaaccat tctatcaatg
    37681 tcgtcatcct caactaaaat tattcccggt ggtatatcaa ttgtcatacc tgatcatttt
    37741 tgaatacaac ttatactgcc atcagcacta ttggttactg aatggccagt atgttgcaaa
    37801 aagcaacatg taagatttca ggaaattttg atggaaaaga ttgaagaaaa caggttcaac
    37861 tataatttat agttcaaaaa gggcaataaa aaaacctcga cagaagcgag gttttaagta
    37921 aaaacttttt agattttatt ttacgcgttc tacataggtg ccggtgcgcg tatcaatttt
    37981 aattttttca ccttcattta caaaaagcgg aacctgaacg acagcacctg tttctaaagt
    38041 tgctgctttt aaagtgtttg ttgcagtatc gcctttaaca ccgggttcgc tgtaagtaat
    38101 taccaattca acaaatgttg gtggctcagc aagtatagga ttatcacctt caaaagcaat
    38161 cttcagcatc atttcttcct ttaaatagct ggcactgtcg cctatcatca cctcaggaat
    38221 ataatgctgc tcataagttt cgttatccat taccaccaag ttctctccat ctttatagag
    38281 atattgcatg gttttgtatt caacacgtgc cacctctact tgttcacctg aacggaagcg
    38341 attttctgca agttttccgt tttttaagtt acgcagtttt gcctgataaa aagcacgaag
    38401 gttgcccggt gtacgatgct gatactctac aacctgatat aaatctccgt tgaatctgat
    38461 tactgaacca attcctatat cgcctgtatc tgccatattc ttttttattt atgtgttaat
    38521 gaaacaaaac aggcaacgtg ataaagcgct gcctgtttat tattttattt actttaactg
    38581 attaagcatt ctcaactaca tcaaaattaa ttttcgcttt gatgtcgcga tgcaatgcaa
    38641 cagctgcact gtatgttcct gttttacgaa tgtgttcttc aggcatttcg atttgcttct
    38701 tatcaatact ataacccaat ttcttcaatg agtcagcaat gttttgagaa gttacagaac
    38761 cataaagctt gccgttttcg cctaccttta caggtattgt cagggtaacg cttgaaatag
    38821 catcagccaa tttctctgca tcacctttga tttttgccat cttgtgcgca cgttgtttca
    38881 ttgtttctgt atgcactttt ttgttggctt cgctggcaat aacagccatt cccttaggga
    38941 taaggtaatt caggccgtag ccatttttta ccttcacgat ttcatcggca tagccgagat
    39001 ttttcacatc ttgttttaat ataatttcca ttgttgcctc ctcttatttt aacaaatctc
    39061 caacataagg catcagggca agatggcgcg cacgtttaac agcctgacca actttacgct
    39121 ggtattttaa tgatgtaccg gttattctgc gtggtaaaag tttgccttgt tcattaacaa
    39181 acttaatcag gaatgtagca tctttatagt caatatactt gattccaact ttcttgaaac
    39241 ggcagtattt cttctttgtg gtgtcaaccg ctaccgggtt gaggtaccta atttctcctt
    39301 gttttgagct catgattcct tcgtttaatt agcagttgct gtttcttgtt ctttcttctt
    39361 caactctgca ttctttctct tcttttcatt gtaagcaatg gcatgcttat cgagcgcaac
    39421 agtcaggaat cgcatcacac gctcatcgcg tttgtattcg acttccaatt tgttgatgag
    39481 ttcaccgctt gacttaaatt cgatcagata atagaagccc gtgttcttct tctgaatcgg
    39541 ataagcaagc ttgcgtaatc cccaattgtt ctcatgaaca atcatggcac cgttgtcggt
    39601 gaggattttc ctgaatttgt caaccgcttc attcatctgc tcattgctga gtacgggtgt
    39661 aagaatgaat acggtttcgt actggtttga catttgttta tatatatttc agtttaatag
    39721 ccttttttaa gcggacggca aagatagtgt tattattaat ttcttgaaat agaattttta
    39781 ctttttttta agatactcat cctaagcgaa tcgctacaat aaattacttt aagtaaaata
    39841 tattaaaaat ttgaaaatca actatataat aaaataattc agatgaataa gaatgatcaa
    39901 atatttaatt agtagcgtaa agcctcaatt gtgtctagcc gcgaagcttt catggcagga
    39961 taaaaacctg aggcaatgcc aataaaacca catagcaaca atccggcaat aatccagttc
    40021 caaggcacca caaaaacagc attcatagcc atgcccatca ggttgccgac caacacacct
    40081 aaaataactc ctgctacgcc acccaagagg caaataacaa ttgcttcaat caaaaactgc
    40141 tttaagatta caagccttgt tgcaccaagt gattttctga ttcctatttc gcgggttcgc
    40201 tctgtgacag agacaagcat aatattcatc aatgcaattg ctgcaccaag caatgtgata
    40261 agtccaatca gcgttgctgc aatacttaca ttcttcaact gatcaattac aattgctgca
    40321 atgctgtcgc gttttttttt ttaaaaattg cggatggccc ataccgcccc aaccaatgcc
    40381 accccccaga ttttataaaa aaagatctta tagccaacca aaaatgcggc aaaaaaaaat
    40441 acaaaaaatg gtcaaaaaaa aaaccacaan nnnnnnnnng ttctgaatag tcctgttgct
    40501 tcatcaactg ctgcttcaag gcctgacaca tgattcacct taacactaac tgtccatgaa
    40561 cgattatcgc tgccatagtt ttgcttaccg ttgattaatg aaatcaaaca tactttatca
    40621 ccaccaaaac ccatgctgtt acctttctcg gcaagtacgc caataattct atattttctg
    40681 ttgtctatat aaacttcttt gtcaacagca tcagcaccag gaaaaagttt actctttatt
    40741 tcagagccaa tcatcactac attatcgcca cgcatagctt ctgtctgcgt catattacga
    40801 cctgttgcaa ttgtataacc tccggtttgt aaatagtttt catcaatagc cataaccaaa
    40861 atattcggat tggttttttc tgaacccact cgacaaactg ccactccatt acacattgta
    40921 gaaatactaa taattgaagg ataggtattg cctgatttaa acaacactgc ctgctgaagt
    40981 gtaatttctt taaatctttt ggcctttttg cctgaattac ccaacctgat tcccaaacct
    41041 gaatttctta tggtgaatgt gtttgctccc atactggtaa agttttcatt gatggatgcc
    41101 ttaaggcctt cgatggctgt aagtattcct accaaagcca ttatgcctat ggcaatgatt
    41161 aacactgtca gtattgttct gagcaactga gactttacag catgtagagc caaaagtata
    41221 ttttcaaaaa tgggcatggc gctaaattac gattaatctt tgccccgaaa agtttttaac
    41281 tcataaatga aagttaattt tttttcaaaa aaatttaagt tttgaaaatt caaacatata
    41341 tgacgaagaa ttttccagag ttaatgtgaa caattaaata aaaatttgct agtttcaata
    41401 atattgccca cttttacaaa gcggtaaatg attgccgcac aaaacaaagt tgaatcataa
    41461 aaatgaagaa ataaagtaga ataaaattgt aaaaaaaata taagcgtttc gctttaaaat
    41521 tcctgtcata gaaatcttgc aaaattttaa cagcacagga gagtaaaagt ggaaacgcct
    41581 gcgtaaaacg cgggcgttcc ttttgcttcg tgctgtaggt ttgcaagaac ctctatgacg
    41641 agcatgggaa ttagcccgcg ttttaatttt taaccacccc catacaaaat ccaaaaaatc
    41701 attgtaattt agtcacaccc caaaaaccca ataatatgaa aaaccaaatc caagcaactg
    41761 cattggccat tgtaatctgc ctgtttacct tctttacaag agcgcaatca caatcagtaa
    41821 atattgcctt aacactgcct accgacacca taggtgcctg cgagagcaat gtttacgccc
    41881 tttcattttc cggagcttca ggtgcaaggg tagtcattac tgcatcgctt agcaatgcac
    41941 caccggcaaa ttgtatcaat gcaagcccca tctatctgga ttttattact gcatcaaaca
    42001 atgtgagcaa tgtagttacc tacaacaacg gacaaactat gagttttacc gtaaccaaca
    42061 acaacgctgt aacactatcc tatcatgctt ttattgactg ctctgttata tccgatgcac
    42121 cacagggaaa cagcaatatt aattttatac aaacatttac agattcctct gccactaact
    42181 atactttatc tgtcaacaat cagggcaatg tgcacacctc tctcaatgtg cagacaccca
    42241 ctcttgttgc cttatcatcc aacctcaacc aggttgccta ctacaaaaca gattcttttc
    42301 tttatttcta ctatcagaat accggattca ccaaactcaa cttcaacttt acttttacac
    42361 ccgatgcaac aaactattgc caacggatta caacagggca accggaatat aatattgggt
    42421 taaacggcaa ctttgttgca acagcaccca acactgctat ggtgctgaac attggcgaca
    42481 cgcttgttgt caggcaggct gttcaggctg ttgcatgttt agataaaatc cctgcctgcg
    42541 ggcctgtagc tgcgcagttg caatggacat gcaacaacaa cattccatca aatattttct
    42601 gcaccaattg caaaaatggt atccccgtaa actactacat taacagtggc gatgcaccgg
    42661 ttgtgaaaat agaacgcata gcacctgccg actctttgta tgacttcagt tgcctgaacg
    42721 atacggtaaa ctttgttcac tggcaatatc gtgttaccaa cagtggcatg ggagccatgg
    42781 attctttgtt ctttaacata aaccaatcct ttccaacgct caataatgcc ttgcctaatt
    42841 ataatgcact gtcactaatt ccgttcccta gttttagctg gcagcaaaat acaggaagcc
    42901 tttgcactgt tgctgtggat accctgcacc gcagcgactg gctgtgcaaa aatttagttg
    42961 ccgatgccct gtttgaagcg gctttcagaa taaaaaactt cggcaatgca gataccgtta
    43021 ttattgcttt taataccctg cgttgctccg aagaagatac caacctgttt aacgttcaaa
    43081 aaaattataa ccactgggcg tttaattctg ttaagggaag aaaagtttgt ggcgatggtg
    43141 tgtctgcaga ctataatata ggcttagcca acactacgtc catcagcacc catgccgttg
    43201 gtgggtcggc agatgtttct caaaatttaa ttttcagccc cacggtgaca gacctttccg
    43261 tagcaccggg tgccaccttt ggcgacagtg ctcttttaaa agtgcgttta aaaggactct
    43321 tgccctcacc ttatgattat caactttatg gttgcagtgt gcctgtaaac aactgcctga
    43381 tgaacggctg gctcagagca gaagtacact gcgacaccaa ccttcgcatt gccaatcccg
    43441 ctatgcatgg gcagttggtt tactatcgta cttcgcaagg cgacactctt gttataaaac
    43501 ccgattatgt ttttgctgct gtggacacca acctttgtga agccggaaac tacaactact
    43561 attttaacat gaacagtgca ggctttaaag catttttaga cagtgcccat tttgaattta
    43621 ccgtgcaggc ctgctgc
//
LOCUS       DAOOOI010000013        32865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_239_length_32865_cov_21.375069, whole genome shotgun sequence.
ACCESSION   DAOOOI010000013 DAOOOI010000000
VERSION     DAOOOI010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 32865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32865
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_239_length_32865_cov_21.375069"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..1096
                     /locus_tag="PKX59_03565"
     CDS             332..1096
                     /locus_tag="PKX59_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89977.1"
                     /translation="MKHLIWLVLIYIMSAFSVKAQDPTTLIQRTPIKSWYAMNSGEFI
                     FSAGELKISDKAVYDPFGSINTIADPDNKIRFSGFFHTSQELHYNFSNVFGFYTGLAL
                     RNVGFINTMKVGGEKLTIKQRSYSLGIPVALKLGNMQTYFLTTGIEPEIMFAYKRKLF
                     YDGDKSKKSQWFSNDVNVFNPSLFAQVNFKGGMYIRVKYYLNDFLTSNTESFSLPDKP
                     VSVSYKVDSSKLYYVSIGTSIKNRKMKKPIKTSVGI"
     gene            complement(1127..1987)
                     /locus_tag="PKX59_03570"
     CDS             complement(1127..1987)
                     /locus_tag="PKX59_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HOZ89978.1"
                     /translation="MITLQNDILQISIKKQGAELCSLINLKNAIEYLWQAKPEWAKHS
                     PVLFPIVGQLKDNEYKWQDKTYKMERHGFARNMPFNLIDNDSLSATFELTDNETTYHQ
                     YPFKFSLQLKYGLNGNTLTISNKIKNNSDFIMPFSIGAHPAFNIPLSIDETYSDYYLQ
                     FNKKENNGNWKLENGLLKNETPFFNNTSEIPLSKSLFENDALVFKNLKSDLIELKSKN
                     NTHGLSVTTANFPYLGIWAAKNADFICIEPWLGIADSVNASGNILEKEGIILLNQQQS
                     FSCAFSINVF"
     gene            2121..2552
                     /locus_tag="PKX59_03575"
     CDS             2121..2552
                     /locus_tag="PKX59_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89979.1"
                     /translation="MIKSFFCLLFLLSIQLADAQHCQIKGKVLEKKSISPLDSVKIEI
                     YEGSLMYKIIYSDKGGRFDTKMFQVISNVSFRISRQGYERRVIRFNKLKGNNVIFMDI
                     SLRKITAEEEAKIQHKEEKKHKTKSKRKKYARPGKRIFLPF"
     gene            2661..3455
                     /locus_tag="PKX59_03580"
     CDS             2661..3455
                     /locus_tag="PKX59_03580"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOZ89980.1"
                     /translation="MGVFLLVTSKELMMFSFAAFLLAFAFLFFIFYRSKREAEVQIEK
                     SRLQLQVAQVEMKALRAQVNPHFMFNALNSIYILIADKEMQKAGNYVLKLGTLIRKIL
                     ENSNHQNISLKDDCEVIDLYIQVECMRLNYSFNYQLTINETIDNESVMVPPMIIQPIV
                     ENAIWHGLKNRKDGGLLTINVDSTKDELVFEIKDNGCKADARMSEDEKLRNAKRKSMG
                     MQITKERLDIINQSNNANARIIEKEIIGGDGSYQGKVVKLYLPLLD"
     gene            complement(3467..4192)
                     /gene="lptB"
                     /locus_tag="PKX59_03585"
     CDS             complement(3467..4192)
                     /gene="lptB"
                     /locus_tag="PKX59_03585"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOZ89981.1"
                     /translation="MLLRAENLVKIYKKRTVVNNVSIQVAQGEIVGLLGPNGAGKTTT
                     FYMTVGLIKPNSGKIFLDDNDITALPMYKRAQSGIGYLAQEASVFRKLSVEDNIKAVL
                     EMTKLSQEEQDAKTEELLNEFGLQRIRKSRGDLLSGGERRRTEIARALAVSPKFILLD
                     EPFAGVDPIAVEDIQSIVAKLKEKNIGILITDHNVHETLTITDRAYLLYEGKILKAGT
                     AEDLANDEQVRKVYLGQNFELRN"
     gene            complement(4229..6694)
                     /locus_tag="PKX59_03590"
     CDS             complement(4229..6694)
                     /locus_tag="PKX59_03590"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOZ89982.1"
                     /translation="MKTIFQSLAGIALFLLFHSTSLAQLTRISGKVTDALTGEPIPFA
                     SVVFKKSTIGVNTNIDGIYTLESDTPSDSISAQFLGYIPVSLPVRKGQTQTINFLLKA
                     SKVELKEAVIHAGENPANIIIKKVIANRDKNNFEKLNSYSFETYNKLEFDLTNITDKF
                     KNRKIFKPFAFIFDNIDSTTTNEKPFLPIFITESLSDRYLSNNPKDKREIIKASKVSG
                     VENETVTQFLGDMYQNINIYDNFMDVFGKKFVSPISSLGLIYYKYYLLDSLYMGNNHC
                     YKLKFKPRRPEDLTFTGEMFITDTTWAVKKVNMRISNKANINFVEDCAVVKEYEFVDS
                     TMWMLSKDLLVIEFAARDKGLGLIGRKSTSYKNFDLNKPIPPNVMKGSQDVVVLDSAL
                     YKDNSFWDTARHDSLNEREKKIYTMVDTIKSLPAYKTYVDVITLFVTGYKTLGKVDLG
                     PYFTVFSFNEIEGYRFRLGGKTNQDFSKHLRLEGFVAYGTKNEQFNYSFGTRYLFTTK
                     PRMGVGFKYRHDVMQLGQSDNAFQDDNILASLFRRSASNKLTYTESEKIYFEKEWLSG
                     VSGRITFEHNEFIPLGKLDYRYYTNENESNIRDKINTSDISAYFRFAYREKFVESKHG
                     RVSLGSKYPVLQAVYTQGLKGVLRSDFDYQKLIVKVDDYLYTAPIGYLYYVLEAGKVW
                     GKLPYPLLEVHRGNESYFYDYLSFNLMNYYEFVSDQYFAFYATHHFEGFFLDKIPLLK
                     KLKWREVASLRGVYGSMSQKNHDLMVAPTYNTLSKKPYLEAGFGVENILKIIRVDFIY
                     RLSYLDNPDISKFGIRGSLQLTF"
     gene            6838..8379
                     /locus_tag="PKX59_03595"
     CDS             6838..8379
                     /locus_tag="PKX59_03595"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOZ89983.1"
                     /translation="MKDKLSLLEQKKKEALAGGGAKRIDDQHKKGKLTARERIHFLLD
                     EGSFEEIGMLVTHRATDFGLAKEKYLGDGVVTGYGTIHGRLVYVFSQDFTVFGGSLSE
                     THAEKICRIMDLAMQNGAPVIGLNDSGGARIQEGVVSLGGYADIFYRNTLASGVIPQL
                     SAIMGPCAGGAVYSPAITDFIMMVQNTSYMFVTGPNVVKTVTHEEVSAEELGGASTHS
                     TKSGVTHFSCANEMECIQYLKTLLSYIPSNCEEMPPSVAYSEANEVREGLNTIVPENP
                     NQPYDIREVVAQVADENSFFEVHKDFAENIVVGFARLAGRSIGVVANQPMFLAGVLDN
                     HSSTKAARFVRFCDCFNIPLLVFEDVPGFLPGTDQEWNAIITNGAKLLYAFSEATVPR
                     ITVITRKAYGGAYDVMNSKHIGADMNYAWPSAEIAVMGAKGAAEIIFKKEISKADNPA
                     EKLKEKEQEYIELFANPYKAAERGYIDEVIEPAQTREKLIKAFKMLENKAAKLPKKKH
                     GNIPL"
     gene            complement(8469..10928)
                     /locus_tag="PKX59_03600"
     CDS             complement(8469..10928)
                     /locus_tag="PKX59_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ89984.1"
                     /translation="MKQINKKGRSILIMLAIMLSGLSSMAQNCNANFQYFAGPGGVVT
                     FMDSSFASGNITGYTWTFGDGTSGTGQQVSHTYNGVGPYTVCLFITTSNGCSDSTCAL
                     VSLNPCNISGVILYDSLQQTLTVTASGGVAPYTYNWTNGQTGMSISVGNPGMYCCVIT
                     DANGCAYTACYMVGGSGCSAAFITNIMGNVVVFGNNSQSFASLLWNFGDGTTSVATSP
                     THTYAQAGTYYPCLTVYDGNGAICSQYCDTIVIVPQAGNTVICGNVFQDMNANGFNDN
                     EPGFANTPVMIWGSGMQYTAMPDSNGNYSINVPPGTYNIVYCVQQPYSLTLPLDSGGC
                     GFYQVTIAAGDTICGFNFGVSLNSSSIEGYVFVDSNTNGIKDAGEAGIAYQPVQIGNL
                     TSYTDLTGKYERYVPIGTYTVSYTPTGVYASYPLTTASSNSVSVVGVGITYQGGNYGL
                     DLPAGSTNLAVNLLPHTTVTPGFPAWYDIQVCNIGINPTGATVTMNYDPGLTYTFGSP
                     VPTSVNTTAHVITWNLPTMTSGQCLYIQADFSASTSYQIGDPTFEFCSAYPTSGTDID
                     LTNNTDTVHQIVTGSWDPNNKLSVQTNNNNPVQQIVSSINSNQEIEYTINFQNLGTAP
                     AVNVVVVDNLSTDLVASSYQLTGASHNVSVSRNGMNVIYNFQNIMLPDAISNEPMSHG
                     FVSYKINAVNGLAAGTQIADFSRIYFDFNSPVTTNIAVVTMVNPTGINEQSEANLQSV
                     YPNPVSDVATVQFTLGQNSLVGLELFDAAGRSTTLMPEVMMQAGLHKTNLNANQLSEG
                     LYLLKLTVNGKSSVMKVTIKH"
     gene            complement(11053..12006)
                     /locus_tag="PKX59_03605"
     CDS             complement(11053..12006)
                     /locus_tag="PKX59_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOZ89985.1"
                     /translation="MRTAILIATIFTTLNISCATGQKNSSSNEPDMKEQLKNGTNIYF
                     ENKTISTDLNFTNLLQPVVESNGINRCYVNSAITFKQCTFKGKITGYATDQSGKSTMT
                     YFQKSVTFIDCIFEDDINLRGAIFSAPANFQGCSFVKKADFQECHFYTDALLNETKYR
                     EEAFFQNAVFMKKMILMNARFESNVYFQNAVFYFDAQFSNAEFQKYADFTVCTFQMGV
                     FFNYCKFQQQAVFSSSVFSKRFEILSTTAQQLDMRKCHFGFVPKLMKSNIVSELDFSG
                     SHFTDGIPDLKETSAATILASEIVLCDKSYSQEQFLKASGK"
     gene            complement(12109..13821)
                     /locus_tag="PKX59_03610"
     CDS             complement(12109..13821)
                     /locus_tag="PKX59_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOZ89986.1"
                     /translation="MKWIKRLFLVTLALSLTISACKKEKDDSPSPTPPTPIPPTPSGN
                     TPKNLGWNGGDNPNSVPTSVTNTPVFNSGTLPTSVSLLPQFPPIGDQGQYGTCVAWAA
                     GYNMKTVIDAIQQNLNASDLTNTNKQTSPKDVFLAIPDAKKGQNCSGTNFSDAMDLLL
                     NRGAATMQTVPYTNLGNCANSGVQSNWTQEANQRKIKNYRKVSPNIQSIKEQLANKIP
                     VVIGAKLSDNFMTWNSDAVYNSNSTYNQVGQHSYHAIIISGYDDSKGANGAFKVVNSW
                     GTNWGDVGYIWIDYNFLVNEFVFDQNFYTATTDGGTNPPSPTPTPSGGVDLASWVFGD
                     ETTYGTSQTQYYNERVVYWNLYNIGTSGAASSSNWSLYYIYFNAYDANDYGVLFYDEF
                     NTSIAQNTYQVDPNDPNHIILNIDIPANNDLGNVGFGTQQLYQVYYTPSTLNGEYYIA
                     MIADAENAFTEDNEQNNFFYTTDQYPKTFQNGYSGRITAQMQSTNATNYVEYKFINKL
                     QPSKHNLTSSIYNTVVTKQRPNAYSSEEIGQMLKWKKKTGDLDRKLAEFKMRQKGNGG
                     FGAK"
     gene            13976..15163
                     /locus_tag="PKX59_03615"
     CDS             13976..15163
                     /locus_tag="PKX59_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOZ89987.1"
                     /translation="MNIDKIKQLAEIAFNIVLADRRHLHKNPELSFKEFQTQQYVWER
                     LDEIGISNKKKIAETGIIAMIEGKNPSAKIIALRADMDALPIQETNKTDYVSQNAGVM
                     HACGHDVHTSSLLGVASVLQQLKSEFSGTVKLLFQPGEEKLPGGASLMIKEGALKNPS
                     VSGILGQHVMPMIETGKVGFRSGVYMASTDEIYVTVKGKGGHGAIPHQNIDPVLIASH
                     IIVALQQIVSRNANPIMPSVLSFGKVMANGATNVIPDSVYLEGTFRTLDEKWRSDAHT
                     KMIRMANAIAEGMGGSCEFKIIRGYPALVNNPQLTASARKNAEAYLGKENVLDLDIWM
                     AAEDFAFYSQETNACFYRLGTRNEANGITSSVHTSTFDIDEKALLTGVGLMSYLALCE
                     LEQ"
     gene            complement(15205..18072)
                     /locus_tag="PKX59_03620"
     CDS             complement(15205..18072)
                     /locus_tag="PKX59_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOZ89988.1"
                     /translation="MKNNLYSKLGCLLLLAPAFSFAQLSFTNSNSRLASPAFHSGCPV
                     SVVDWNNDGLDDIIRLDNGKHCYVDVHKTNQQYQRIFLGTFSGSTWAMAVADLDKNGY
                     KDVVAGGAFGSVEILKTNNSGTGATLSSLSNSNFFLQNLTMADFDNDGWIDIFCCDDN
                     AASHIYLNNGSGGFGAQSNIINFNLPNGSDNSGNYGSVWTDFDNDGDLDLYIAKCRQS
                     VNNPSDPRRINVLFVNNGNGTFTENAAAYNINIGWQTWTAAFGDIDNDGDLDLMITNH
                     DHNSQIFQNDGTGVYTDITSSTGFTVSDITPIESVMEDFDNDGFVDIFVTGSDSRFWR
                     NNGNSTFTLVENLFDNNDMESFAIGDANHDGWIDVMASYANIYTIPSNIDDVLWINNG
                     SKQNIIDGVAGLPTNNFFNVVLEGNTTNKDAIGARALIYGTWGVQLREVRAGESYGTN
                     NSAILHFGLGTATAIDSMKVIWPSGVSQTISNPSINQFLSIKENDCVSPEAIVTASGP
                     LVLCTSGQALTLTAPAGFNYLWSDSSTAQSITITQTGEYNVTISQPGNNCSATSATMV
                     VIPSPNQTPAITALGETVFCNGGSVQIQGPIGFSSYLWSNGDTTQTATVTTNGPVTLT
                     IQGICQSFTSDPINVTVNTVTDPIANDVILSAAGSATLNATVGNNISWYATNAGGTPL
                     ATGPSYTTPVLTANTTYYMQATDTFGGGIYAVGLPTWSGSGSGYSGNTTNATTEFNVT
                     KNCTLKSVKVFTNVAGIRRIELKDASSTVINYVDVNVSPDSQVVTLNFPLTPGMGYTL
                     GTNTTTNQNNLGTDSPKFKRNNSGVNYPYTLSDALSITNSSNGGQYLYYFYDWQVEKE
                     SWECVSNMVPVNVYITTGIKENDRSILSAYPNPVSDKLNISNPFGTVKATLFDATSRV
                     VGSYVLQNGENIFNIESLNAGVYQLQVNHDGEISNIRLVKN"
     gene            complement(18132..20063)
                     /locus_tag="PKX59_03625"
     CDS             complement(18132..20063)
                     /locus_tag="PKX59_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89989.1"
                     /translation="MKNKIIVLFLISVAIIGISAIPFQPKKAMAYHSESEIAFFKNHS
                     MTPIAPGEFFLTPDNCKGCHGFDTLGIANVDANGQDVNLHDDWETSMMGLSAVDPFWR
                     AKVSHETMVNPAHATELQNLCITCHAPAGHYSAIYKGQQHYVMSDLLNDSLGLSGVSC
                     TACHAIGDSSSLGQLFTGQIPYDTNKVLFGPFISPVAGPMQLYVGMIPTYSPHVSEGK
                     FCSPCHTLLSNTVDLNGIPTGGTFIEQATYHEWLNSTYPSQATVCQSCHMPQIEDAVI
                     LANGNVGLPGRTPFNLHQFAGANSFMVNLIKQNKTALGVSAPDYNFDSTLAAITHMLT
                     TQSLDVQASLAAITSDTAFIDVDLTNKAGHKFPSGYPSRRAVLQMIVTKANGDTLFAS
                     GLFDANYEMQNVASPFEQHHDIINSQTQNQIYEMVMGDVNGTVTTVLERAASHLKDNR
                     IPPAGFSTLHYAYDTCKIVGSALTDVDFNKNGATQGTGHDIVHYHIPLNNYSGTINVY
                     TAVYYQTLPPAFLSDMSNYSSPEINTFLTMYANADKSPVKVDADTLLNISTGIKENIL
                     TTINISPNPTRDGKVLLSGIGYNSFQLEVFDQNGKKINVILTRKNNSIEVQLPTAKGI
                     YFIRLNVNGQVKSYKILRI"
     gene            complement(20226..22694)
                     /locus_tag="PKX59_03630"
     CDS             complement(20226..22694)
                     /locus_tag="PKX59_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOZ89990.1"
                     /translation="MNKYLRYFVFLLLTCLALLLIAMGLSVLLQDKIKQSVIEEINKS
                     LNTPASVSEIDFSVLRFFPNASLTLKNVVIKGNPWQDTKRPLLKAGKIDLVLSITSIF
                     SSTIQLKKIEISDAELNILTNENGQKNDQIFKQGNDNNSFAAEFKKVILKNVNLSIEN
                     KPSNFILQTHLNKSLASGNFKDKTFELKTETDLFAEIIQHENISYIKQKPIQLNGVVS
                     VDLRNDIYAFNDLKIQLANLNINAKGKIEKRNETFLDLKFTATKANASELLSILPSNW
                     VAPQILNYRYHGNINFDTHINGVASSKQSPTIEINFGTNKTDIIPDNENYALKNVALK
                     GFYSNRKSGKNISVLSLKNISARIEGKPIIGEVYLENLKNPYFNIQLDADISLASLSR
                     YLPADFTEEQQGSVSLHGRISGQADKKDTYRSNGTLTLNNLKFKLKKRNIAVNSISGK
                     INFNNADVKVESLKILAGQSDVVINANILNFYNYIFRDNQTITINGIVESHFLDAGEL
                     LAGNQSTDTSDFDLPAYLNLNASLSADVVKFRKFNAKDLSGNISLQDKILTAKQLSFN
                     AMEGHIALDGTINTNFKDEVHISSNAKIESINIERLFFETGSFGQQVLTEKNIRGTLT
                     ASVQLATYWTKQLEVKQSRTIASADLSINDGALIDFKPMLKLSRFVKGSDLQNVRFSK
                     LQNTIRIHEQKILIPTMDIRSTAMNMTISGTHGFDNFVDYKIQMKLSQLLGKKVRQQN
                     TEFGTIEDDGSGGLNLFLTMKGPLDNPKFSYDRKGVEKKITESVKSGKDDFLKIIKEE
                     ISGKKPPKEETPKKQKELQIDYDE"
     gene            complement(22888..23490)
                     /locus_tag="PKX59_03635"
     CDS             complement(22888..23490)
                     /locus_tag="PKX59_03635"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOZ89991.1"
                     /translation="MSFQLPPLPYAPDALEPHIDTRTMEIHHDKHHQAYVTNLNNAIA
                     GTDAEKMSIEDICKNISKLPMAVRNNGGGHYNHSLFWTIMAPNAGGAPKGKLADAINS
                     AFGSFDEFKTKFNQAAATRFGSGWAWLIKNAAGKLEVCSTPNQDNPLMDVAEVKGTPL
                     LGIDVWEHAYYLHYQNRRPDYCTAFWNVVNWDEVSNRFEK"
     gene            23719..23991
                     /locus_tag="PKX59_03640"
     CDS             23719..23991
                     /locus_tag="PKX59_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ89992.1"
                     /translation="MKKVLALVMAAGMFSIVACGPSAEEKQKMEETAKAAQDSIAAAA
                     QQAASAAEQAATDAAQVATDAAQTVDSTAGAVVNQATEAVKEAATK"
     gene            complement(24042..25166)
                     /gene="dprA"
                     /locus_tag="PKX59_03645"
     CDS             complement(24042..25166)
                     /gene="dprA"
                     /locus_tag="PKX59_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOZ89993.1"
                     /translation="MENNLTTDVLQYQLALTLLPGVGDITAKNLVGYCGGAEAIFKEK
                     QQALLKIPNVGQIVADKIKNGKEQSLKRAEEEIRFIEKNKIKPLFFTSDQYPKRLKAC
                     NDSPIMIFVKGNGNLNPEKCIAFVGTRKASDYGKTTTEKLIADLAETDGMMIVSGLAF
                     GIDITAHKASLKCKIPTAGIVAHGLDTLYPAEHRNTAEKMQAEGAVISEFMSKTNADK
                     ENFPKRNRIIAGMADAVVVVESGIKGGSLITADIANSYNRDVFAIPGQVDKEYSKGCN
                     ALIRDNKANLIQSAADIEYFMGWDKTDVKSIPKQARLFEDLNPEEKQLVDFLKENSKV
                     DIDTICFRSGVKLNRIPALLLNLELKGVIKNYPGKVYDLI"
     gene            25294..27504
                     /locus_tag="PKX59_03650"
     CDS             25294..27504
                     /locus_tag="PKX59_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ89994.1"
                     /translation="MNAQQQRVSGKVTDGEGELPGVAVYADESHATTSDAKGEFVLML
                     DKGSWTLKAKLLGYETFSKVILVGDSNYVQIHLIPLSKQLQSVVVSAGKFEQKLEEVT
                     VTMETIKPKSFETTGDNNVETALEHVTGLNVNDGQANIRGGSGFSYGAGSRVLVLVDD
                     MPMLSADANDAKWSFLPIENLEQIEIIKGASSALYGSSAMNGVINFRTGYAKSEPVTK
                     IVQYNGIYDSPRRKEIKWWGKETQMLSGANMFHAQKFGQWDFVAGGHYLLDEGFRKGE
                     TEKRARVNINTRYRFPKIEGLAVGVNANYMESFGGLFIIWNDDTTGAYIPSGGVNDST
                     STLSNYHTVRYNIDPFITYHSKKGFSAKWRSRYFRTLNYNDTDQEAFADLYYSEVQLQ
                     QRFEKWNLVLTGGGVITDGGVRSDLYGSRKTYNKALYAQADKKFFDRLNLSLGWRWEQ
                     FRFRYSKAESKPVIRAGANLRLLRATYLRSSYGQGYRFPSIAEKYVKTRVGSIVIYPN
                     DSIFSESGWSSEIGLRQLFAVGKQFSGYVDASVFRSEYKNMLEFAFGSYGDPFKDPLF
                     GLGFKSLNIGNTRIDGMEFELGVQATVLKKINLQFGGGYTYIDPRLTDFDSLKASTGT
                     ITYNFLKYRYRNLFKGYGSIAYGKVSLAVNCRYNSFMENIDVVFNELLPGVKRYRAEH
                     DKGDWIYDANISYKLNSQIALSVISRNLFNHEYSGIPSQLEAPRMWLFQFVITP"
     gene            complement(27550..28218)
                     /locus_tag="PKX59_03655"
     CDS             complement(27550..28218)
                     /locus_tag="PKX59_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ89995.1"
                     /translation="MNKTIPDKPLRVIIVDDNKNMRESVALLLQSTPSLLLIAEFDSG
                     EECIKQVDTLKPDVILMDIDMPKLSGIETVKIIRKQFPGLPILMLTGFEDDDKVFDSI
                     CAGANGYILKNTSMELLIHHIHEVYTGGAPMTPVIARKVLTRFAKTQVTETKNEDYNL
                     SKREMEVLELLVKGKSYKTIATELKVSYETVHTHIRKIYHKLHVKSIGEAVSKTIKNK
                     ILNS"
     gene            complement(28267..31347)
                     /locus_tag="PKX59_03660"
     CDS             complement(28267..31347)
                     /locus_tag="PKX59_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOZ89996.1"
                     /translation="MIKFFKSLLLFLVFTLPALADSLTINLNEFTANDGLSDNQTNCV
                     IQDKYGFIWIGTKDGLNRFDGKSFQVFKFQSDKNSVSGNNITCLEVTDDSLLWIGTAS
                     NGFCSYNYKTNTFQNYHARNSPLRAEYINDLAFDKVRNCIWVAMNNGGLQQFDLKNKK
                     LNPYVYSINSYYAVAVKDTISLFAGIIESLKKLDNVGRNKGMVGDTATTINTIYIDND
                     LNVWCGAWDNALHLFNIHGKRLQSYIFDGTSKFKKSGEEIISISSDASNNIWCGTKSS
                     GIFIFNKASKSFINNINFSHQINTRVNELFRDSQNRIWIATENGLLVYDQHRNQFRTE
                     MLPVPKGVNACKVFDRLITDKGLDLVITQCGLFYKKSPNESYKFKTVIYRNEEQELYS
                     ILMTKNGDIYIGSNRTVFKFNPENISFNTIDFNKNLKETSFYFNGASLVNSLTSFKQD
                     NSELIAASYYGNYIVLIDFDNRNLYYLIHENTKISDNLENLSRKIWVDSKNNFWVCGR
                     SKGLVQLKFKSDFSLSKYAADNDSMLPITVMPTNWNMLPNSIPIKIKNVFDIAERKDG
                     TYWLSTQGAGLVLFSKENKTSPFKIIDSDITSMQGIKSTGDSLLWIITSSGLLQYNEK
                     TNFFKLYDASSGLSQNIFGYFFQNKPGDNNTLSVGFNGGFISYNPSQLLSSTEKPNLQ
                     FTKLWVMDKPSDSLLFSDIKLHHNQNFLKVYVSPNIYTNSDKITYFYRLSGIDDNWRS
                     NDNNPIITYTNLPPGKFRLDVKAISNNNLQSDIKTLSFVIVPPFTSTIWFYGIVVFSI
                     GAFVYFIYRYRINQLLRLQEVRNNIARDLHDDIGSTLGSIHLYSQIADNKMKQDKHHE
                     IPGILEKIKKSSKEIIEKTGDTVWVVKASNDNMSNLSLRMETYAASILGDADINFNFI
                     VEENIQNLKINMAQRKNLFLIFKEAIHNIVKYSHCTQVDIHLSRNRGMTFLKIKDNGC
                     GIDMANNHSQTNGNGGNGLKNMASRAKDLNAEFQINSAPGKGTEITISLKQHAI"
     gene            complement(31496..>32865)
                     /locus_tag="PKX59_03665"
     CDS             complement(31496..>32865)
                     /locus_tag="PKX59_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ89997.1"
                     /translation="AAGGCGVANNGNNTMHVSSVFNPTGGAAYDAGGLCGLLYCPQTN
                     RMAQSPVINCTGKSNISISFNYIEFGDGTIDNATLWYYDGAAWAQITDMPKTACCGGA
                     CNGFRQGKWTAFSMVLPASANNNANVRIGLKWENNDDGVGTDPSFAIDDISLSSLTSS
                     QFVAEYMRANPQITYSNIFDPTLDHISQCEFWTLNQIAGSSSRNVTLSWDASSCGVTL
                     LSDLRVARWRSVGTIWNNLGNGSTTGNVSAGTITTSTADNLFGPFTLSSASSENPLPV
                     ELLNFTAQLNNDQVELRWSTVSEINSDYFEIERAAVDLNFIPINKTQAAGFSNQLLRY
                     HATDSKPLARKSFYRLRQFDFNGESHLSNSVAVELNNYEQLQINYAVFTNKNNLYFKV
                     TGNVEKNLTAEVYDINGAIISTQELNGTENIFHVQTQNLANGIYVLKISNSNSSVTKR
                     FSVHD"
BASE COUNT        10241 a         5636 c         6147 g        10841 t
ORIGIN      
        1 aaaagaacaa agcgataaaa acgaatacca cttttaatgc actgaataat ttttgcggga
       61 taatttgttt ttccattttt agtgattttt gttattaatt aaaattttac ccaagcaatt
      121 taggataaca tcgttgctca ttgaattgta atttggtgaa tggcaaattt tttagggcga
      181 aaatcaaaaa acataaagcg acatattccg tccgtgcttt tatttacgcg attatgctgt
      241 aaatagccgc acatgtggtc tttaaagcca tgtttttgat tgttactaaa tgcgaatgga
      301 attactattt ttggcataaa ttaaaataca gatgaaacat ttaatttggc ttgttctgat
      361 atatataatg agtgctttta gtgttaaagc acaagatcca acaaccttaa ttcaacgaac
      421 tccaataaaa tcatggtatg ccatgaattc cggagaattt attttttctg caggtgaatt
      481 gaagatatct gataaggcag tttatgatcc atttggtagt ataaacacta ttgctgaccc
      541 tgataataaa atccgtttta gtggtttttt tcataccagt caggaacttc attataattt
      601 ttcgaatgtg tttggatttt atacaggact tgcattgcga aatgttggat ttatcaatac
      661 catgaaagtg ggcggagaaa aattaaccat caaacaacgc tcctattcat taggtattcc
      721 tgtagcatta aagttaggca atatgcaaac ctattttttg acaacaggaa tcgagcctga
      781 aattatgttt gcctataaac gaaaattgtt ttatgatggc gataagtcaa aaaaatcaca
      841 atggttcagt aatgatgtca acgtattcaa tccatcattg tttgcacaag taaatttcaa
      901 gggtggtatg tacattagag ttaaatatta tctcaacgat tttcttacaa gcaatacaga
      961 aagtttcagt ctgcccgata aacctgtttc tgtcagctat aaagttgatt caagtaaact
     1021 ttactatgta tcaataggaa ctagtattaa aaacaggaag atgaaaaagc ctattaaaac
     1081 ttcagtaggt atttgatgga tgataaacac aagttcttaa ttttttttag aaaacattta
     1141 tagaaaaagc acaagaaaag gattgttgtt ggtttaatag aatgattcct tctttttcca
     1201 aaatattacc cgaagcattt acactatcag ctatgccaag ccaaggttca atgcaaatga
     1261 aatctgcatt ttttgctgcc catattccaa gataaggaaa gtttgctgtt gtgacactta
     1321 aaccatgagt attattctta ctttttaatt caattaaatc tgattttaga tttttaaaaa
     1381 ccaatgcatc attttcaaat aaactttttg aaagtggaat ctctgatgtg ttattaaaaa
     1441 aaggtgtttc attcttcagc aagccatttt cgagtttcca atttccgtta ttttcttttt
     1501 tattgaattg taagtaataa tcgctgtagg tttcatctat gcttaatgga atattaaaag
     1561 caggatgtgc accaatagaa aaaggcatga taaaatcact gttgttctta attttattgc
     1621 tgatagttaa cgtatttcca tttaagccat atttaagttg aagcgaaaat ttgaatggat
     1681 actgatgata agtagtttca ttatcggtta attcaaatgt tgcagagaga gaatcattat
     1741 caattaaatt gaaaggcata tttcgtgcaa aaccatggcg ttccattttg tacgttttgt
     1801 cctgccattt atattcattg tctttaagct gaccgacaat tggaaataat acgggtgaat
     1861 gtttagccca ttcaggtttt gcctgccata aatattcaat agcatttttt aaatttatca
     1921 aactgcataa ttcagctccc tgctttttta tgctgatttg aaggatatca ttttgtaaag
     1981 taatcatatc aaaatggtga aatttaaatc aatttagccc tcaatgataa gaaaacatta
     2041 attttagcgg caattaagtt ttattcagaa cattttcagt attttacgta taagataaat
     2101 gtcgcttttg tcaaacaata atgattaaat catttttctg tttattgttt ttattgtcaa
     2161 tacaattggc agatgcgcag cattgtcaga taaaaggtaa ggttttagag aagaagagta
     2221 tatcgccact cgattctgtt aagatagaaa tttacgaagg cagccttatg tataagataa
     2281 tttattcaga taaaggtgga cggtttgata ccaagatgtt tcaggtaata agcaatgtga
     2341 gtttcaggat atcgaggcaa ggatatgaaa gacgagttat caggtttaat aaattgaaag
     2401 ggaataatgt gatttttatg gatatttcac tcaggaaaat tacagcagaa gaagaggcta
     2461 aaattcaaca taaggaagag aaaaagcata aaacaaagtc aaaacgaaaa aaatatgcca
     2521 ggccgggaaa acgtatattt ttacctttct aagttttttt aataaaggta ttatttttat
     2581 tcaattttag ttattttggc caatagattt cagccgtgta gttcatcggt tacaagtttt
     2641 aatccgagtt aagtttttat atgggtgttt ttttattggt aacctccaag gagctaatga
     2701 tgtttagctt tgctgctttc ttattggcgt ttgctttttt gttttttatc ttttaccgtt
     2761 caaaacgcga ggcagaagtt cagatagaaa aaagcagatt gcaacttcag gtagctcaag
     2821 tagaaatgaa ggcgctgcgt gcacaggtga atccacattt tatgttcaat gcattaaaca
     2881 gcatttacat tttaattgct gataaggaaa tgcaaaaggc aggaaactat gttttaaagt
     2941 taggcacatt aatccgtaag attcttgaaa actcaaacca tcagaatatt tctttgaagg
     3001 acgattgtga agttattgat ctttatattc aggttgaatg tatgaggctt aattatagtt
     3061 ttaattatca actgacaatt aatgaaacta tagataatga aagtgtgatg gttccgccaa
     3121 tgattattca gcctattgta gagaatgcaa tttggcatgg tttaaaaaac agaaaagatg
     3181 gtggtcttct taccattaat gttgacagca caaaagatga attagttttt gaaataaagg
     3241 ataatggttg taaagcagat gcccgaatgt cagaagatga aaaactgcgc aatgccaaac
     3301 gaaaatcaat gggaatgcaa atcactaagg agcgtttaga tattatcaat caaagcaaca
     3361 atgccaatgc cagaataatt gaaaaagaaa ttattggtgg tgatggcagt tatcagggta
     3421 aggttgtgaa actatatctt cctcttttgg attaaaatct tgctatttaa ttcctaagct
     3481 caaaattctg tccgagatat acttttcgaa cctgctcatc attagcaagg tcttcggcag
     3541 taccggcttt taaaatttta ccttcgtaca gcagataggc gcggtcagtt atagttagtg
     3601 tttcgtgaac gttgtgatca gtaattaaaa ttccgatatt tttttcttta agctttgcta
     3661 caattgattg tatgtcttca acagcaatgg ggtcaacacc tgcaaatggc tcatcgagca
     3721 gaataaattt aggactcaca gccaaagcac gtgcaatctc tgttcgtctg cgttcgccac
     3781 cggataatag atcacctcgg ctttttctta tcctctgtaa accgaattca ttaagaagtt
     3841 cttctgtctt ggcatcctgt tcttcttgcg aaagttttgt catctccaaa acagccttaa
     3901 tattatcttc aacacttaat ttgcgaaaaa cagaagcttc ctgagctaaa tagccaatac
     3961 cggactgtgc ccttttatac attggcaagg ctgtaatatc attatcatca agaaaaattt
     4021 taccgctatt gggttttatc aagccaacag tcatataaaa cgttgtggtt ttaccggcac
     4081 cattggggcc aagcaaacca acaatttcac cttgtgctac ctgaatagac acattgttaa
     4141 caacggttcg ttttttataa atcttaacta agttttctgc gcgtaacaac atttttgtca
     4201 attcaatatg gtgcaaagat aacagctatc agaatgtcaa ttgcaaactt cctctgattc
     4261 cgaatttaga aatgtcggga ttgtctaaat aactgagacg ataaataaaa tcaacacgaa
     4321 ttatctttaa aatattttct actccaaaac ctgcctcaag atagggtttt ttagaaagtg
     4381 tattgtaggt aggggcaacc attaaatcat gatttttttg cgacatgctg ccataaacac
     4441 cacgaaggct tgccacttcg cgccacttta attttttaag caatggaatt ttatctaaga
     4501 agaatccttc aaaatgatgt gttgcataaa atgcaaaata ttggtcgctg acaaattcat
     4561 aataattcat caggttgaaa gatagataat cataaaaata ggattcattg ccgcgatgca
     4621 cttccagtaa cggataaggt aatttacccc aaacttttcc ggcttctaaa acatagtaca
     4681 gatagccgat tggtgcagta tataaataat catccacctt aacaatcagt ttctgataat
     4741 caaagtcact tcgtaaaaca ccttttaatc cttgtgtata tacagcctgc agcaccggat
     4801 atttacttcc aagacttaca cgtccatgct tgctttcaac aaatttttca cgataggcaa
     4861 acctgaagta agcagaaata tcagacgtgt taattttatc tctgatgttg ctttcatttt
     4921 catttgtata gtaacgataa tctaattttc ctagtggtat aaactcattg tgttcaaaag
     4981 tgattcgtcc actgacaccg gaaagccatt ctttttcaaa ataaattttt tcactttcag
     5041 tataagttaa tttgttggag gccgatcgcc tgaaaagcga tgccaaaata ttatcgtcct
     5101 gaaatgcatt atcactttgc ccaagttgca tcacatcgtg cctgtattta aagccaacac
     5161 ccatgcgtgg ttttgttgta aataagtaac gagtgccaaa actatagttg aactgttcgt
     5221 tttttgtacc ataggcaaca aagccttcta aacgcagatg tttactaaaa tcctgattcg
     5281 tttttccacc aagccgaaaa cgataacctt caatttcatt aaaactaaat actgtgaaat
     5341 acggtcccaa atcaactttg cccaatgttt tatatccggt aacaaaaagt gtaattacat
     5401 caacataagt tttataagca ggtaatgatt taatggtgtc aaccatggtg taaattttct
     5461 tttcacgctc gtttaacgaa tcatgcctag ccgtatccca aaaactattg tctttgtaaa
     5521 gtgcactatc aagtacaacc acatcctgcg agcctttcat tacatttggt ggaataggtt
     5581 tgttcaaatc aaagttttta taagatgttg actttcgtcc tatgagacca agtcctttat
     5641 cgcgagcagc aaattctatc accaacaaat cttttgaaag catccacatg gtgctgtcca
     5701 caaattcata ttcttttaca acagcacagt cttccacaaa gttgatattg gctttattgg
     5761 aaatacgcat gtttactttt ttgactgccc aagtagtgtc ggtaataaac atctcacctg
     5821 taaaagtaag gtcttcgggt ctgcgtggtt taaacttaag tttatagcaa tgattgttac
     5881 ccatatacaa actgtcgagc agataatatt tgtaataaat tagtccaaga ctcgaaatag
     5941 ggctaacaaa ttttttgccg aacacatcca taaaattatc gtagatgttt atgttttggt
     6001 acatatcacc caaaaactgt gttaccgttt cgttctcaac accggaaacc ttacttgctt
     6061 taatgatttc gcgtttgtct tttggattgt tagacagata gcggtcactt aaagattctg
     6121 taataaaaat aggcagaaaa ggtttttcgt tggtagtagt actatcaata ttgtcaaaga
     6181 taaatgcaaa gggtttaaaa attttacggt ttttaaattt gtcggtaatg ttggttaaat
     6241 caaattctaa tttattgtat gtttcgaagg aataactgtt cagtttttca aaattatttt
     6301 tatccctgtt tgcaataact tttttaatga tgatgttggc aggattttct cctgcatgaa
     6361 tcactgcttc tttcagttca accttgctgg cttttaaaag aaaattgatg gtttgtgttt
     6421 gaccttttcg aaccggaagc gatacaggaa tatatcctaa aaactgagca gagatgctgt
     6481 cgcttggtgt atctgattct aaagtgtaga tgccatcaat attggtgttg acacctatgg
     6541 ttgatttttt aaaaacaaca ctggcaaagg gtataggctc accggtgagt gcatcggtaa
     6601 ccttacctga aattctggta agttgtgcca acgaagtgct gtgaaaaaga aggaataaag
     6661 caatgcctgc aagcgattgg aaaatggttt tcacaaattg atttatgtag aattttttac
     6721 cttgctaaga acggaaaaaa gttcgattcc ttgcctgaca gggctttagt tttatgttaa
     6781 acgggcagtt cttcatttag ttttttaagt ttgcacaata gaataattaa cacagcagtg
     6841 aaagataaat tatcattact tgaacagaaa aagaaggaag cacttgcagg tggcggtgcc
     6901 aagcggattg acgatcagca taagaaagga aaactaacgg caagagagcg gatacatttt
     6961 ttgcttgatg aaggcagttt tgaagagata ggcatgttag taacgcatcg tgcaacagac
     7021 tttggcctgg caaaagagaa atatcttggt gatggtgttg ttaccggtta cggaacaatt
     7081 catggtaggt tggtgtatgt tttttctcag gactttactg tatttggtgg ctccctgagt
     7141 gagacacatg cagaaaaaat ttgccgtatt atggatttgg ccatgcaaaa tggtgcacct
     7201 gtaatcggct taaatgatag tggaggtgcc cgcattcagg aaggtgttgt ttcattggga
     7261 ggctatgcag atatttttta tcgtaacaca ttggcatcag gagttattcc tcaattgagt
     7321 gccattatgg gaccttgtgc aggtggggct gtttactcac ctgcaataac agattttatt
     7381 atgatggtac aaaacacgtc atatatgttt gttaccggac ctaatgttgt aaaaacagtt
     7441 acccatgagg aagtaagtgc agaagagtta ggtggagcat caacccattc aacaaaatcc
     7501 ggagtgacac atttttcttg tgccaatgag atggaatgta ttcagtatct taaaacttta
     7561 ctgagttata ttccgtcaaa ttgcgaggag atgccgccat cagttgcata ttcagaagct
     7621 aatgaagtgc gtgagggttt gaatacgatt gtacctgaaa atccaaatca gccttacgac
     7681 atacgagagg tagtagcgca ggtagcagac gaaaattctt tttttgaagt acataaagac
     7741 tttgccgaaa atattgttgt tggctttgcg cgtttagccg gtcgtagcat tggcgtagtt
     7801 gccaatcagc ctatgtttct ggcaggtgtt ttagataatc attcatcaac taaggcagca
     7861 cgctttgttc gtttttgcga ttgttttaat attccattgt tggtgtttga agatgttccg
     7921 ggatttttgc ccggtaccga tcaggagtgg aatgccatca ttactaacgg agctaaattg
     7981 ttgtatgcat ttagtgaggc aacggtgcca agaataactg tcatcacacg taaagcttat
     8041 ggtggtgctt atgatgtgat gaactccaaa catattggtg ccgacatgaa ctatgcatgg
     8101 ccatctgcag agattgcagt gatgggagca aaaggtgcag cagaaattat tttcaaaaaa
     8161 gaaattagta aggctgataa tcctgctgaa aagctgaaag aaaaagaaca ggaatatatt
     8221 gagctttttg ccaatccata caaagcagca gaacgtggct atattgatga agtgattgag
     8281 cctgcacaga caagagaaaa attgattaag gcatttaaaa tgcttgaaaa taaagcagcc
     8341 aaattgccta agaagaagca tggaaatatt ccgttgtagg aaggaatatt tcttatttat
     8401 tacaatattg ttttaaacaa aaagccctct gaaaagaggg cttttttaat gatgaccaaa
     8461 aatcaaggtt aatgtttaat tgtaaccttc attactgatg attttccatt gactgtcaac
     8521 ttaagaagat ataagccttc tgaaagctga ttggcattta gatttgtttt atgcaagcct
     8581 gcctgcatca tgacttcggg cattaatgtg gtgctgcgtc ctgccgcatc aaacaattca
     8641 agtcctacta aagaattttg ccctaatgta aactgaacag ttgcaacatc actgacagga
     8701 ttaggataaa cactttgtaa gttggcttcg ctttgttcgt taattccagt aggattcacc
     8761 atagtaacaa ctgcaatgtt agtagttacc ggactattga agtcgaaata aattcttgag
     8821 aaatcagcaa tttgagttcc tgcagcaagt ccgttaacgg cattgatttt atatgacaca
     8881 aatccatgac tcatcggttc attgctgatg gcatcaggaa gcataatgtt ctgaaaatta
     8941 taaataacat tcatgccatt gcggcttaca gaaacattgt ggcttgcacc ggttaactga
     9001 taagaacttg caaccaaatc agtagagagg ttgtcaacta ccactacatt cactgcaggt
     9061 gctgttccta aattctggaa gttgatagta tattcaattt cctgatttga atttattgaa
     9121 gacacaatct gctgaacagg attgttgttg ttggtttgaa cagaaagttt attgttcgga
     9181 tcccatgagc ctgtaacaat ctgatgaacg gtatcagtat tattagttaa atcaatatca
     9241 gttcctgatg ttggataggc gctgcagaac tcaaacgttg ggtcgccaat ctgataagaa
     9301 gttgaagcac tgaagtcggc ctgtatatag aggcattgtc ctgaagtcat tgttggaaga
     9361 ttccaggtaa tgacgtgcgc agttgtattt acagatgttg gtacaggact accaaatgta
     9421 taagttaatc ccgggtcgta gttcatagta actgttgcac ctgttgggtt gatgccaatg
     9481 ttgcaaacct gaatatcgta ccatgcaggg aagccgggtg taacagtagt atgcggtaat
     9541 aaatttactg caagatttgt tgaacctgcg ggtaaatcaa gaccatagtt tccaccctga
     9601 taagtaatac caacgcctac gacacttaca ctgttactac ttgcagttgt caatggataa
     9661 gatgcataaa ctcctgttgg cgtataggaa actgtataag ttccaatcgg aacataacgc
     9721 tcatattttc ctgttaaatc agtgtaggaa gtaaggtttc caatctgcac aggttgataa
     9781 gcaatacctg cttcgcctgc atctttgatt ccattagtgt tgctgtcaac aaaaacataa
     9841 ccttctattg aagaactgtt gagagaaaca ccaaaattaa atccacatat agtatcacct
     9901 gctgcaattg ttacctgata aaaaccgcaa cctcctgaat caagtggtaa ggtaagactg
     9961 taaggttgtt gaacacagta aacaatatta taagttccgg gaggaacatt gatgctgtaa
    10021 ttaccatttg aatcgggcat tgcagtgtat tgcataccgg atccccaaat cattaccggt
    10081 gtattggcaa agcccggttc attatcatta aatccattgg cattcatgtc ctgaaagaca
    10141 ttgccgcaaa taactgtatt gcctgcttgt ggtacaatta caatcgtatc gcaatactgt
    10201 gaacatatag caccgttgcc atcataaact gtcagacaag gataataggt tcctgcctga
    10261 gcataagtgt gtgttggtga tgtagcaaca gaagtggtgc catcaccaaa gttccagagt
    10321 agtgatgcaa aactctgtga gttgttacca aacaccacaa cattacccat aatgttggtg
    10381 ataaatgctg cactgcaacc ggagccaccc accatatagc atgctgtata ggcacagccg
    10441 ttagcatcgg taatcacaca gcaatacatt cctggattac caacagaaat gctcattcct
    10501 gtttgaccat ttgtccagtt gtaggtgtaa ggtgctacac cgcctgatgc tgtaactgtt
    10561 aatgtttgtt gcagtgaatc ataaagaatg acaccggata tattacaggg gtttaaacta
    10621 actaatgcgc aggtagaatc gctgcaacca ttagatgttg taataaataa acatactgtg
    10681 taaggaccca caccattata agtatgcgat acttgctgac ctgttcctga tgtgccatca
    10741 ccaaaagtcc aggtataacc ggtgatattg cctgatgcaa aacttgagtc cataaaagtt
    10801 actactccac cgggtccggc aaagtattga aagtttgcat tacaattttg tgccatactg
    10861 ctcaaaccgg agagcatgat ggcaagcatg ataaggatgc ttcttccttt tttgttaatc
    10921 tgtttcatga tatttgaatt ttgatacaaa gaaaatgtaa cggtatatta ctgcaaaacg
    10981 gattttgaat aaatcttact tgcttaaagt agctaaaatg ttctattttc aataatgaaa
    11041 tcaaaaataa gcttacttac ctgaggcttt taaaaactgc tcctgagagt aacttttatc
    11101 gcataaaaca atctctgatg ctaatattgt tgcagcagaa gtttctttta agtcaggaat
    11161 gccgtctgta aaatggcttc cgctgaagtc aagttctgaa acaatattac ttttcatcaa
    11221 tttggggaca aaaccaaaat gacattttct catgtccaat tgctgagctg tggtactaag
    11281 tatttcgaag cgtttactaa acacactact actgaaaact gcctgttgtt gaaatttgca
    11341 gtagttgaag aaaacaccca tctgaaaagt gcaaacagta aaatcagcat atttctgaaa
    11401 ctctgcattg ctaaactgcg catcaaaata aaaaacagca ttttgaaaat atacatttga
    11461 ttcaaatcgt gcattcataa gaatcatttt tttcataaat acagcatttt gaaagaatgc
    11521 ttcttcgcga tatttggttt catttaataa agcatctgta taaaaatgac attcctgaaa
    11581 atcggctttt tttacaaagc tacaaccttg aaaatttgcc ggagcagaaa atattgctcc
    11641 tcttaaattt atatcatctt caaaaataca atcaatgaat gttacacttt tttgaaaata
    11701 agtcattgtt gacttgcctg attgatcagt agcatatcct gtaatttttc ctttgaatgt
    11761 acactgttta aaagtaattg cagagttgac ataacatcgg tttatgccat tgctttcaac
    11821 aacaggttgc agcagatttg taaaatttaa atcagtactg atagttttat tttcaaaata
    11881 aatattagtt ccgtttttta gttgttcttt catatccggt tcgtttgaag agctgttttt
    11941 ttgtcctgta gcacaggaaa tatttagtgt tgtaaaaatt gtagctataa gaattgctgt
    12001 acgcataata ttttttttac aagtttaaaa atcatttggt gaaacagaaa aaatcccggc
    12061 agaaattttc ttaccgggat gatattgttt gcggtttaaa tgttgaattt atttggcacc
    12121 aaagcctccg ttgccttttt gacgcatttt aaattctgct aatttacggt caaggtcacc
    12181 ggtttttttc ttccatttaa gcatctgtcc aatttcttca gatgaataag catttggacg
    12241 ttgtttggta acaacagtat tatagatgct tgaagttaaa ttgtgctttg atggttgaag
    12301 tttattaatg aatttgtact ctacatagtt tgttgcattg gttgattgca tttgcgcagt
    12361 aatacgccca ctgtagccat tttgaaatgt ttttggatac tgatcagttg tgtagaagaa
    12421 gttattttgt tcattatctt ctgtaaatgc attttctgca tcagcaatca ttgcaatata
    12481 atattctcca ttcagagttg aaggggtgta gtaaacctga tacaattgtt gtgttccaaa
    12541 accaacatta ccaagatcgt tattagcagg tatgtcaata ttcagaataa tgtgattggg
    12601 atcattcggg tcaacctgat aagtattttg agcaatagat gtattgaact catcatagaa
    12661 aagcacacca taatcattgg catcataagc attaaagtaa atataataca aactccagtt
    12721 tgaagaactt gctgctcccg aagttccaat gttgtataga ttccaataaa caacacgttc
    12781 gttgtaatat tgtgtttgtg atgtaccata agttgtttca tcaccaaaca cccaagaggc
    12841 taggtcaacg cctcctgaag gtgttggagt aggtgaagga ggatttgttc ctccatcggt
    12901 tgtggcggta taaaagtttt gatcgaatac gaattcattc acaagaaaat tataatctat
    12961 ccagatatat ccaacatcgc cccaattggt tccccatgag tttacaactt tgaatgcacc
    13021 attggcacct ttactatcat cataaccgga aatgataatt gcatgatagg aatgttgtcc
    13081 aacctgattg taagttgaat ttgaattgta aacggcatcg ctgttccaag tcatgaaatt
    13141 gtcggaaagc ttagctccaa tcactaccgg aattttattg gctaattgct ctttaatact
    13201 ttgaatattc ggactgactt ttctgtaatt tttaatcttg cgttgattcg cttcctgtgt
    13261 ccagttactt tgcacaccgg agttggcaca attgcctaga ttggtatatg gaactgtttg
    13321 catagtagct gcgccacgat ttaataataa atccattgca tcagaaaagt ttgtaccact
    13381 gcaattctga cctttttttg catccggtat agcaagaaaa acatccttag gactcgtttg
    13441 cttgtttgta ttcgtcaggt cgcttgcatt aagattttgc tggattgcat caataacagt
    13501 tttcatattg taacccgcag cccatgctac acaggttcca tactgaccct gatcaccaat
    13561 tggaggaaac tgtggtaaca aactaactga tgttggtaat gttccactat taaatacagg
    13621 ggtattggta accgaggtag gcacagaatt ggggttatca ccaccgttcc atcctaaatt
    13681 ttttggcgta ttccccgatg gtgttggagg aataggagta ggaggcgtag gtgaagggct
    13741 atcatctttt tctttcttgc aagcactaat tgtaagtgat agtgccaatg ttactaagaa
    13801 taatctttta atccatttca ttttttaaaa ttttaaattg atgagtgcta caaagtagaa
    13861 tataacaaac tggattgtca atcaccaaat ctggtgattt ttatagctga aaacaatact
    13921 taatttgatg tataaaaacg taaaatattg aacaataaag ttttaatttt gacacatgaa
    13981 tattgataaa ataaagcaac ttgcagaaat agcgtttaac atagtgcttg cagacagacg
    14041 gcatttgcat aaaaatcctg aactttcatt taaggaattt cagacacaac agtatgtttg
    14101 ggagagattg gatgaaatag gaataagtaa taaaaagaaa atagcggaaa ccggaattat
    14161 tgcaatgatt gaaggtaaaa atccttcggc aaaaattatt gcactgcgtg ctgatatgga
    14221 tgcgcttccc attcaggaaa ccaataaaac agattatgtt tcacaaaatg caggtgtcat
    14281 gcatgcatgt ggacatgatg tgcatacttc tagtcttctg ggggttgctt ctgttttgca
    14341 acaattaaag agtgaatttt caggaactgt aaaattattg tttcagcccg gagaagaaaa
    14401 actccccggt ggtgcttcat taatgataaa agaaggggca ttaaaaaatc cttcggtttc
    14461 aggaatttta ggacaacatg taatgccaat gattgaaacc ggaaaagtag gtttcagaag
    14521 tggtgtttat atggcaagta cagacgagat ttatgtaact gtaaaaggaa agggtggaca
    14581 tggagctatt ccgcatcaga atattgatcc ggtgctcata gcatcacata taattgttgc
    14641 gttacaacag attgtaagta gaaatgcaaa ccccattatg ccaagtgttt tgtcgtttgg
    14701 aaaagtaatg gccaacggag caaccaatgt tattcctgac agtgtttatc ttgaaggaac
    14761 tttcagaaca cttgatgaaa aatggcgaag tgatgcacat acaaaaatga ttcgtatggc
    14821 aaatgctatt gcagaaggta tgggtggaag ttgtgaattc aaaattattc gaggttatcc
    14881 tgcattagta aacaatccgc aactgacggc atcagcaaga aaaaatgccg aagcttattt
    14941 ggggaaggag aatgttttag atcttgacat ctggatggca gcagaagatt ttgcatttta
    15001 ttcgcaggaa accaatgctt gcttttaccg tttagggaca agaaatgaag ccaatggaat
    15061 tacttcatca gtgcatactt caacatttga tattgatgag aaagcattgt tgacaggtgt
    15121 aggattgatg tcatacttgg cactttgcga attagaacaa taaaaaaacc gccccttttc
    15181 agaggcggct gctaagtttt ttgtttagtt tttaactaag cgtatattac ttatttcacc
    15241 atcatgattt acctgaagtt ggtaaacacc tgcattgagt gattcaatat taaaaatatt
    15301 ttctccattt tgtaaaacat aactacctac tacacgcgat gtagcatcaa acaatgttgc
    15361 ctttacagtt ccgaatggat tagaaatatt taatttatca ctaacaggat ttggataagc
    15421 agataaaata cttctgtcat tttcttttat accggtggtg atatacacat tcacaggaac
    15481 catattgcta acacactccc acgattcttt ttctacctgc cagtcataga agtaatataa
    15541 gtactgccca ccattacttg agtttgtaat gcttagcgca tcagataaag tatatggata
    15601 gtttacaccg gagttgtttc ttttgaattt aggtgaatca gttccaagat tattctgatt
    15661 ggttgtggta tttgtgccaa gtgtataacc cattcccgga gttaacggga agtttagtgt
    15721 tactacctgc gaatcaggag aaacgtttac atcaacataa ttgataacag tggatgaggc
    15781 atcttttaat tcaattcttc tgattccggc aacattggta aatactttga cagattttaa
    15841 agtacagttt tttgtaacat taaattctgt tgtagcattg gtagtatttc cagaataccc
    15901 tgaaccagaa cctgaccatg ttggtaatcc tactgcgtaa atgccaccac cgaaggtgtc
    15961 agttgcctgc atatagtatg ttgtgttcgc ggtaagaaca ggtgtagtgt aacttggtcc
    16021 tgttgctaaa ggtgtacctc ctgcattggt tgcataccat gaaatgttat tacccactgt
    16081 agcatttaat gtagcacttc cggctgcaga taaaataaca tcgtttgcta tcggatcagt
    16141 aactgtattt acagtaacat taattggatc agaggtaaat gactgacaaa tgccctgaat
    16201 agtcaatgtt acaggcccat tagttgttac agtagctgtt tgtgttgtat cgccatttga
    16261 ccaaaggtag ctgctgaatc ctataggtcc ctgaatctga acactacctc cattgcaaaa
    16321 cacagtttcg ccaagcgcag taatagcagg tgtctgattt ggtgatggaa taacaaccat
    16381 tgtagctgat gtagcagaac aattgtttcc gggttgtgag atagttacat tgtattcacc
    16441 tgtttgggtg atagtaatac tttgtgcagt tgaactgtcg ctccataagt aattaaatcc
    16501 tgcaggtgca gttaaagtta atgcctggcc acttgtacac aatactaaag gaccagatgc
    16561 agtaacgatt gcctcaggtg aaacacaatc attttcttta attgataaaa attgattaat
    16621 tgaaggattg cttattgttt gacttacacc acttggccag attactttca tagaatcaat
    16681 agccgtggct gtacctaaac caaaatgtaa aattgcacta ttattggttc cataactttc
    16741 acctgctcgc acttcgcgca gttgaactcc ccacgttcca taaatcagtg cacgcgcacc
    16801 aatagcatct ttattggtag tatttccttc aagcaccaca ttaaaaaaat tgttggtcgg
    16861 tagtccggca acaccatcaa taatattttg tttactaccg ttgttaatcc acaatacatc
    16921 atcaatgttt gaagggatgg tgtaaatatt tgcataactt gccataacat caatccagcc
    16981 atcatggttt gcatcaccaa tagcaaatga ttccatgtca ttgttatcaa ataaattttc
    17041 aactaaagta aaagtgctgt taccattatt tctccaaaag cgtgaatcgc ttccggttac
    17101 gaaaatgtca acaaatccat cattgtcaaa atcttccatt acactttcaa taggagtgat
    17161 gtcagaaaca gtaaatcctg ttgatgatgt aatatcagta taaacacccg ttccatcatt
    17221 ctgaaaaatc tgactgttgt ggtcgtgatt tgtaatcatc aaatcaagat cgccatcatt
    17281 gtcaatatca ccgaaagccg ctgtccaagt ttgccagcca atattaatgt tgtaagcagc
    17341 agcattttca gtaaaagtac cattaccatt atttacaaat agtacattaa tacgtctagg
    17401 gtcggaagga ttatttacag attgtctgca tttcgcaatg tataaatcaa ggtcgccatc
    17461 gttatcaaaa tcggtccaca cacttccgta gtttcctgaa ttatcagaac cattgggtag
    17521 gttaaagttg ataatatttg actgtgcacc aaatccaccg gagccattat tcagataaat
    17581 atgacttgct gcattatcat cgcaacaaaa aatatcaatc caaccatcgt tgtcaaaatc
    17641 ggccatggtt aaattctgca agaagaaatt tgaattgctt aatgaagata gtgtagcacc
    17701 ggtaccacta ttgtttgttt ttaatatttc aacacttcca aaagcgccac cggcaacaac
    17761 atctttgtaa ccatttttat ctaagtcggc aacagccatt gcccacgtac ttcctgaaaa
    17821 tgtaccaaga aatatgcgtt gatattgctg attggtttta tgcacatcaa catagcaatg
    17881 tttcccatta tctaaacgaa taatgtcgtc taagccatca ttattccagt caactacaga
    17941 aaccggacag ccactgtgaa atgctggaga tgccagtctt gagttactgt tggtaaatga
    18001 aagttgtgca aaggagaatg caggtgctag gagcagcagg catcctaatt ttgagtaaag
    18061 attatttttc attttaaaca agtttctgat ttaatgtaat ttgtttttat ttataagaga
    18121 tgttatagtt tctaaattcg taatatttta taagatttaa cctgaccatt cacattaagt
    18181 ctgataaagt aaataccttt tgctgtgggc agttgaactt cgattgaatt attttttctg
    18241 gtaagaataa cattaatttt tttaccgttt tgatcaaaca cttctaattg aaatgaattg
    18301 taacctatgc ctgaaagaag aacttttccg tctcttgttg gattgggtga aatattaatg
    18361 gttgttaaaa tattttcttt tattcctgtt gagatattaa gcaatgtatc agcatcaact
    18421 tttacaggac ttttatctgc attggcatac atggtaagaa atgtatttat ttcaggggaa
    18481 gaataattgc tcatgtcact taaaaatgca ggtggcagtg tttgataata aacagcagta
    18541 taaacattaa tggttccact gtaattgttt aatggaatat gataatgaac aatgtcatgt
    18601 cctgttcctt gtgttgcacc atttttatta aaatctacat ctgttaaagc gctaccaaca
    18661 attttacaag tgtcataagc ataatgcaag gttgaaaatc cggcaggagg gatgcggtta
    18721 tctttcagat gtgaagctgc gcgttcaagt acggtggtta cggttccgtt tacatcaccc
    18781 attaccattt cataaatctg attttgtgtt tgtgagttga tgatgtcatg gtgttgctca
    18841 aaaggtgaag ccacgttttg catttcataa ttggcatcaa acagtccgga agcaaacaac
    18901 gtatcgccat tagcttttgt aactatcatc tgtagcacag ctcttcttga aggatatcca
    18961 cttgggaatt tatgccctgc tttgtttgtt aggtcaacat caatgaatgc tgtatctgaa
    19021 gtaatagctg ctaatgaagc ctgcacatcc aatgattgtg ttgtcagcat gtgtgtgata
    19081 gctgcaagtg ttgaatcaaa attatagtca ggtgctgaaa cacccaatgc agttttgttt
    19141 tgcttaatca gatttaccat aaacgagtta gcacctgcaa actgatgcag attaaatgga
    19201 gttcgtccgg gcaaaccaac attgccattt gccagtatta cagcatcttc aatttgtggc
    19261 atgtggcaac tttgacagac tgttgcctgt gatggataag tggaattaag ccattcatga
    19321 taggttgcct gctcaatgaa agtgcctcct gtaggaatac catttaaatc aacagtgttg
    19381 gaaagtaatg tgtgacaggg tgaacaaaat tttccttcac ttacatgtgg gctgtaggta
    19441 ggtatcatgc ctacataaag ttgcatgggt ccggccacag gagaaataaa aggtccaaat
    19501 agaactttat tagtatcata aggaatctga ccagtaaata gctgacctaa actactgctg
    19561 tcaccaatag catggcaagc tgtgcagctt acacctgaaa gaccaagaga gtcgttcaac
    19621 aaatcggaca taacataatg ttgttgtcct ttataaattg cgctatagtg ccctgcaggt
    19681 gcatgacaag taatacataa attctgtaac tcagttgcat gtgccggatt aaccatagtt
    19741 tcgtgactta ccttggcacg ccaaaaaggg tcaactgcag aaagtcccat cattgaggtt
    19801 tcccagtcat catgaaggtt aacatcctgc ccgttggcat caacattagc aattcctaaa
    19861 gtatcaaaac catgacagcc tttgcagtta tctggagtta aaaaaaattc tccgggggca
    19921 atcggtgtca ttgaatgatt tttaaaaaac gctatttcag attcagaatg ataggccata
    19981 gccttttttg gttgaaaagg aatagcgcta atacctataa tggctactga aatcagaaat
    20041 aaaacgatta tcttgttttt catgcctgca ttatcctcaa tattttttta cggattgatg
    20101 gcaataatat caattttttt aatgtgctgc aacagaaatc tttggcaaaa catcaaatta
    20161 ttaacgtcaa taggtttgta atacaagtaa agagaatggt cggatgaaga aataagaaca
    20221 gtccattatt catcataatc aatctgtaac tccttttgtt ttttgggagt ttcttctttt
    20281 ggtggtttct tgccacttat ctcttctttg ataattttta aaaaatcatc cttgccggat
    20341 tttacagatt ctgtaatttt tttctctact cctttacggt catagctgaa tttggggtta
    20401 tctaatggcc ctttcatggt cagaaaaaga tttaacccac cactgccgtc atcttcaatg
    20461 gttccaaatt ctgtattttg ctgacgcact tttttgccga gtagctgaga aagtttcatt
    20521 tgtattttat agtcaacaaa attgtcaaag ccatgtgtgc cacttatggt catgttcatt
    20581 gcagtggagc gaatatccat tgtggggata agtatttttt gttcatgaat tcgaattgtg
    20641 ttttgaagtt tactgaaacg aacattttgc agatcgctgc cttttacaaa acgcgaaagt
    20701 ttaagcattg gcttaaaatc aataagagcg ccatcgttaa tacttaggtc tgctgatgca
    20761 atggtacgac tttgttttac ttcaagttgt tttgtccaat aggtagcaag ttgaacggaa
    20821 gcagtcaatg tgcctctgat atttttttct gtaagcactt gctgaccaaa gcttcctgtt
    20881 tcaaaaaaaa gtctttcaat gtttattgat tcaattttcg cattgcttga tatgtgtact
    20941 tcgtctttaa aatttgtatt aatagtgcca tcaagagcta tgtgaccttc catagcatta
    21001 aatgaaagtt gttttgcagt tagaatctta tcttgcagtg atatgtttcc ggaaaggtct
    21061 ttagcgttga atttcctgaa ttttactaca tctgcactta acgatgcatt cagattaaga
    21121 tatgcaggca aatcaaaatc agaagtgtcg gtagattgat taccggccag caattcaccg
    21181 gcatctaaaa aatgactttc aacaattccg ttaatagtga tagtctgatt atcacgaaat
    21241 atatagttgt aaaaattaag aatatttgca ttgatgacaa catcactttg cccggcaaga
    21301 atttttaaag attcaacttt tacatcagca ttgttaaaat taattttacc actgatgcta
    21361 ttaactgcaa tatttctttt tttaagttta aatttcagat tgtttaatgt cagtgtacca
    21421 ttggagcgat aagtatcttt cttgtctgct tgtccgctaa ttctaccatg aagagaaaca
    21481 gagccttgtt gttcttctgt aaagtcagca ggtaaatagc gacttaaact ggcaaggcta
    21541 atgtcagcat ctaactgtat gttgaagtat ggatttttaa gattttcgag ataaacttcg
    21601 ccaataattg gtttgccttc aattcttgca gaaatatttt tcagactcaa aacactaata
    21661 ttttttccgg atttacgatt agaataaaat ccttttagtg caacattctt caatgcataa
    21721 ttttcattat caggtataat gtcggtttta ttagtgccaa agttaatttc aatggttggt
    21781 gattgttttg atgatgcaac accatttata tgtgtatcaa agtttatgtt accatgatag
    21841 cgatagttta aaatttgcgg tgcaacccaa ttagatggta aaatagaaag tagttctgat
    21901 gcattggctt ttgtagcagt aaatttaaga tcgagaaaag tttcatttct tttttcaatt
    21961 tttccttttg catttatatt taagttggca agttgaattt ttaaatcatt aaatgcataa
    22021 atgtcattac gtaggtcaac cgaaacaaca ccattaagtt gtatgggctt ttgtttgatg
    22081 taagaaatat tttcatgttg aataatttca gcaaacaaat ctgtttctgt tttaagttca
    22141 aatgttttat ccttaaaatt acctgaagca aggcttttgt tcaggtgagt ttgtaagatg
    22201 aaattagatg gtttattttc aatactcaga ttaacatttt taagaattac ttttttgaat
    22261 tcagcagcga aggagttgtt gtcgtttccc tgtttgaaaa tctgatcatt tttttgaccg
    22321 ttttcattgg tcagaatatt taattcagca tcagaaatct ctatcttttt cagctgtatt
    22381 gttgaagaaa atatgctggt aatgctaagc accaaatcaa tttttccggc ttttagcaaa
    22441 gggcgttttg tgtcttgcca aggattgcct tttatgacta catttttaag tgtaagtgat
    22501 gcatttggaa aaaaacgtag tacggaaaaa tcaatttcac taacacttgc aggtgtgttg
    22561 aggcttttat ttatttcttc tattacagat tgtttgattt tatcttgtaa taagactgaa
    22621 agtcccattg caatgagcag tagtgccaag catgtcaaca gtaaaaaaac aaagtagcgc
    22681 aaatacttat tcatgtcctc ttaaaacgtt gcaacttttt taaattgtaa taacgataaa
    22741 aatgtatttt gcaaagtaat tttattagca catagtaaac tatgaaataa ggcgtttgtt
    22801 ttatggagtt ttaaactttt aatgtgcaat aaaaaaggct gccttaattc aattaaggta
    22861 gcctttataa attaattaat cgggaaatta tttctcgaaa cgatttgaca cttcgtccca
    22921 gttaaccaca ttccagaaag cagtacagta atcgggtctg cggttttggt agtgcaggta
    22981 gtaggcatgt tcccaaacat caatacctaa aagcggtgta ccttttactt cagcaacatc
    23041 cattaacgga ttatcctgat tgggggtaga acaaacttca agtttaccgg cagcattttt
    23101 tattaaccag gcccagcctg atccaaaacg agttgctgca gcttgattaa acttcgtttt
    23161 aaattcgtca aacgaaccaa aagcgctgtt tatagcgtca gctaattttc cttttggtgc
    23221 accacctgca ttgggtgcca taatggtcca aaacagtgag tgattgtagt gtccgccacc
    23281 attgttgcgt actgccatcg gtagtttcga aatattttta caaatatctt caatgctcat
    23341 tttttctgca tctgtacctg caatagcatt gtttaaattg gttacataag cctgatggtg
    23401 cttgtcatgg tgaatttcca tggtgcgtgt atcaatatgt ggctctaacg catcgggtgc
    23461 gtatggtagt ggagggagtt gaaaagacat aatatctaat ttaaatgtta agcaacaaag
    23521 gtagtgaaat atgctacctt tgttgtcaat caagtactat tttcagaaga aatacattga
    23581 tacaggggtt gttactctcc ggctaaaatt ataaaacact gataaccaac gaataaaaat
    23641 ttaatataaa acttaatatg cagaggtaaa gcaatttata tttgccgctc aaatttcaaa
    23701 tcaattaatt acaaaacaat gaaaaaagtt cttgccttag ttatggctgc cggaatgttc
    23761 agcatcgttg catgcggtcc ttctgctgaa gaaaaacaaa aaatggaaga aacagctaaa
    23821 gctgctcagg attcaattgc tgctgctgct caacaagctg cttctgctgc tgaacaagct
    23881 gctactgacg ctgctcaggt tgctaccgat gctgcacaaa ctgtagattc aactgcaggt
    23941 gctgtagtaa atcaggctac tgaagctgtt aaagaagctg ctacaaagta atcagccccc
    24001 attttaaaag atataagctc gctttagcgg gcttttcttt tttatatcaa atcataaacc
    24061 tttcccggat aattttttat cacacctttc aattctagat tcaacaacaa tgcaggaata
    24121 cggtttagct taacacccga tcggaaacag attgtatcaa tatcaacctt tgagttttct
    24181 ttcaaaaaat ctaccaactg tttttcttca ggatttaagt cttcaaacaa acgtgcttgt
    24241 ttaggaatag atttcacatc tgttttatcc catcccataa aatattcaat gtcggcagca
    24301 gattgaatta aattggcttt attgtctctt atcaaggcat tgcagccttt tgaatattct
    24361 ttatcaacct gaccgggaat ggcaaaaaca tctctgttat aagagttagc aatgtctgca
    24421 gtaatcaaac taccaccttt aattcccgat tcaacaacca ctacagcatc tgccatgccg
    24481 gcaatgatgc ggtttctctt tggaaaattt tctttgtctg cattagtctt actcataaac
    24541 tcgctgataa cagcaccttc tgcctgcatt ttttctgcag tgttgcgatg ttctgccgga
    24601 tacagtgtat caagaccatg tgcaacaatt cctgctgtag gtattttaca ttttaaactt
    24661 gctttgtgtg ctgtaatgtc tataccaaaa gctagtccgc tgacaatcat cattccatct
    24721 gtttccgcta aatctgcaat gagtttttca gtggttgttt ttccatagtc gctggctttt
    24781 cttgttccta caaatgcaat acatttttcc ggattcagat taccattgcc tttcacaaaa
    24841 atcattattg gcgagtcatt acatgcttta agcctttttg gatattgatc agaagtgaaa
    24901 aatagcggtt ttattttatt tttctcaatg aaacgaattt cttcttcagc cctctttagc
    24961 gactgttctt ttccattttt aattttatct gctactatct gaccaacatt aggaattttt
    25021 agtaatgcct gttgtttttc tttgaaaata gcctctgcac caccacaata cccgactagg
    25081 ttttttgctg taatgtcgcc aacaccaggt aagagtgtca gcgcaagctg atattgtaaa
    25141 acatcggttg ttaaattatt ttccattgtt gactttcaag attttcaaga atagtttagg
    25201 tttgtgcctt cacaatctga atctgcaaag aaaaacatgt ttagccaaag aataacttta
    25261 ttttattttt ttttcgtttt aggaatttat tctgtgaatg cacagcagca acgtgtttct
    25321 ggaaaagtta ccgatggaga gggtgaattg cccggtgtag ctgtttatgc cgatgaaagc
    25381 cacgctacta catcagatgc taaaggtgag tttgttttaa tgcttgataa agggagttgg
    25441 acacttaaag caaaattact cggttacgaa actttcagca aagtaattct agtaggcgat
    25501 tctaactatg ttcaaatcca tttgatacca ttaagtaaac aattgcaatc agttgtagtg
    25561 agtgccggaa agtttgagca gaaactggaa gaggttacag ttacgatgga gaccattaaa
    25621 cctaaaagct ttgaaacaac aggcgacaac aatgttgaaa cagcacttga acatgtaacg
    25681 ggattgaatg taaatgatgg gcaagcaaat attcgcggag gcagtggatt tagttatggt
    25741 gcaggcagca gggtgctagt gctggttgat gatatgccca tgcttagtgc cgatgccaat
    25801 gatgcaaaat ggagtttttt accaattgaa aatttagaac agatagaaat tataaaagga
    25861 gcatcatctg cactttatgg ttcgtcagct atgaatgggg tgataaattt caggacaggt
    25921 tatgctaagt cggagccggt aacaaaaatt gtacagtaca atggaattta tgattcacca
    25981 cgaagaaagg agattaagtg gtggggaaaa gaaactcaga tgttgagtgg agcaaatatg
    26041 tttcatgcgc aaaaatttgg acaatgggat tttgtagcag gtggacatta tctgctcgat
    26101 gaagggttta gaaaaggtga aacagagaag cgagccagag ttaatataaa tacacgatac
    26161 agatttccga agattgaagg actggcagta ggagtaaatg ctaattatat ggaatcattc
    26221 ggtggtcttt tcattatatg gaatgatgat accacaggtg cttatattcc aagtggaggc
    26281 gtgaatgatt caacatctac tttgagtaat tatcacacgg ttagatacaa tattgatcct
    26341 tttattactt atcatagtaa gaaaggtttt agtgcaaagt ggcgctctcg ttatttccgg
    26401 actttgaatt acaatgatac cgatcaggaa gcttttgcag atttatatta ttcagaagtg
    26461 cagttgcaac agcgttttga aaaatggaat ctggtgttaa ccggtggagg tgtgattact
    26521 gatggtggtg tgcgcagtga tttgtatggt agtagaaaaa catataacaa agcattgtat
    26581 gcacaggcag ataagaaatt ttttgacagg ttgaatcttt cattaggctg gcggtgggaa
    26641 caatttcgtt tccgttacag taaggcagaa tccaaacctg taatcagggc aggagcaaac
    26701 ctgcgtttat tacgtgccac gtatttacgc agtagctacg gacaaggata cagatttcca
    26761 tcaattgcag aaaaatatgt aaaaacaaga gtaggttcaa ttgttattta tccaaacgat
    26821 tcaatatttt ctgaaagcgg atggagttcc gagatagggt tgaggcagtt atttgctgtc
    26881 ggcaaacaat tttcaggata tgtggatgca tcggtattca gaagtgagta taagaacatg
    26941 ctagagtttg ccttcggaag ttatggtgat ccttttaaag atcctctgtt tggacttgga
    27001 tttaaatcac tgaatattgg taatactcgt attgatggaa tggagtttga acttggtgtg
    27061 caggcaacag ttcttaaaaa aataaatctt cagtttggag gaggctacac ttatattgat
    27121 ccacgtttga ctgattttga ttctttaaaa gcatcaacag gaaccattac atacaacttc
    27181 ttaaaatatc gttacagaaa tttgtttaaa ggttatggaa gtatagccta tggaaaagtt
    27241 tctttagctg taaattgcag atacaatagc tttatggaaa acattgatgt tgtatttaat
    27301 gaattgcttc caggggttaa aaggtatcgg gccgaacacg ataaaggcga ctggatttat
    27361 gatgcaaaca tcagttataa attaaactcc caaatagcgt tatccgttat ttcaagaaat
    27421 ttgttcaatc acgaatattc cggaattccc tctcaactcg aagcaccaag aatgtggttg
    27481 tttcaatttg taattacacc ttagtgtttt actatcaatg ttaaaatcaa actaaagttg
    27541 tgtaaggcat caactgttca ggattttatt tttaatcgtt tttgaaacag cttcaccgat
    27601 tgacttaaca tgaagtttgt gatatatttt tctgatatga gtgtggactg tttcgtaact
    27661 aactttaagt tctgttgcaa tggttttgta ggacttacct tttactaata attccagtac
    27721 ttccatttcg cgtttgctaa gattgtagtc ttcatttttg gtttcggtaa cttgtgtttt
    27781 agcaaaacga gtcagaactt ttcttgcaat aaccggtgtc attggtgcgc ctccggtata
    27841 gacttcatgg atatggtgaa tcagaagctc catgcttgtg tttttcaaaa tatagccatt
    27901 agcaccagca catattgaat caaacacctt atcatcatct tcaaagccgg tgagcattaa
    27961 aataggtaag ccagggaact gtttgcgaat aatttttact gtttcaatac ctgacaattt
    28021 aggcatatca atatccatta ggataacatc aggttttaag gtgtcaactt gttttataca
    28081 ttcttcaccg gaatcaaatt ctgcaattaa aagcaatgat ggtgtgcttt gcagaagaag
    28141 cgcaacagat tctcgcatgt ttttattgtc atcaacaata atcactctca gtggcttatc
    28201 gggaattgtt ttattcatgc tacaaatttg aatattctta tttgatcttg caatcaccaa
    28261 ttctggttat attgcatgtt gtttcaggct aatagtaatt tctgtaccct tacccggggc
    28321 actgttaatc tggaactcag catttaaatc ttttgctctg gaagccatgt tttttaatcc
    28381 attaccacca tttccatttg tttgcgaatg attattggcc atatctattc cacaaccatt
    28441 gtcttttatt tttaaaaatg tcattccacg atttctgctt aaatggatat caacctgagt
    28501 gcagtgtgaa tattttacaa tattgtgaat agcctcttta aaaatcagaa aaagattctt
    28561 tcgttgtgcc atattaattt tcaggttctg aatattctct tcaacaatga aattaaaatt
    28621 tatatcagca tcgcctaaaa tgcttgcagc ataggtctcc atacgcaatg aaaggttaga
    28681 catgttgtca ttacttgctt taaccaccca aaccgtgtca ccggtttttt caataatttc
    28741 ttttgacgac ttttttattt tctctaaaat tccgggaatt tcatggtgtt tatcttgttt
    28801 cattttatta tcggcaatct gactgtagag gtgaatactg cctaatgtgc tgcctatgtc
    28861 atcatgtaaa tcgcgtgcaa tattgtttct tacttcttgt aatcttagaa gctgattgat
    28921 tctgtaacga taaatgaaat acacaaaggc accaatacta aaaactacaa tgccatagaa
    28981 ccatatcgtg ctggtaaacg gtggtacaat aacaaaagac aaagtcttaa tgtcgctttg
    29041 caaattattg ttcgaaatgg ctttaacatc aagacgaaat tttcccggag gtaaattggt
    29101 gtaggttatt ataggattat tatcgttgct gcgccaatta tcatcaatac ctgacaggcg
    29161 atagaaataa gttattttat cactgttggt ataaatattt ggtgacacat aaacttttaa
    29221 aaaattctga ttgtgatgaa gtttgatgtc agagaaaagt aatgaatcag atggtttgtc
    29281 cattacccat agtttggtaa actgaaggtt tggtttttct gttgaagaga gtaattgtga
    29341 tgggttataa ctgatgaatc caccattaaa gccaacactt agtgtattgt tgtcaccagg
    29401 tttattttga aagaaatagc cgaatatatt ctgacttaaa ccactgcttg catcatacaa
    29461 tttaaaaaaa tttgttttct cattgtattg taaaagacct gatgaggtaa ttatccataa
    29521 taaggagtct cctgtagatt tgattccttg catagatgtg atatcagaat caattatttt
    29581 aaaaggagag gttttatttt ctttactaaa aagtaccaag cctgccccct gtgtcgaaag
    29641 ccaataggtt ccatctttac gttcagcaat atcaaaaaca ttttttattt ttattggaat
    29701 tgagttcggc aacatattcc aatttgttgg catcaccgtt attggtaaca tggaatcatt
    29761 atcagctgca tattttgata aactaaaatc gctcttgaat ttaagttgga caagaccttt
    29821 tgatctgcca caaacccaga aattgttttt tgaatcaacc caaattttgc gacttaaatt
    29881 ctctaaattg tccgatattt ttgtattttc atggataaga tagtacaagt ttcgattatc
    29941 aaagtcaatt aatacaatgt agttgccata gtaagatgca gcaattaatt ctgaattgtc
    30001 ttgtttgaaa ctggtgagag aattgactaa tgatgcacca ttaaagtaaa atgaagtttc
    30061 tttcagattt ttattaaagt ctattgtatt gaatgaaata ttttcaggat taaatttgaa
    30121 aacagttctg ttactgccaa tgtaaatatc accattcttc gtcatcagaa tactatataa
    30181 ttcctgctct tcatttcgat aaataacagt tttgaactta taggattcat ttggagattt
    30241 tttataaaat aaaccacatt gggtaatgac aaggtctaaa cctttgtcag taatgagtct
    30301 gtcgaatact ttgcaagcat taactccttt tggcacagga agcatttctg ttctgaattg
    30361 atttctatgc tgatcataca ctaacaaacc gttttctgtt gcaatccaga ttctgttttg
    30421 tgaatcacga aacaattcgt ttacacgggt attaatctga tgagaaaaat ttatattgtt
    30481 tataaatgat ttagaagctt tgttaaaaat aaatatgcct gatgattttg ttccacacca
    30541 gatgttattg ctggcatcag aagaaattga aataatctct tcgccagatt ttttaaattt
    30601 agatgttccg tcaaagatgt acgattggag gcgcttacca tggatattga atagatgcag
    30661 tgcattatcc catgcgccac accaaacatt caaatcatta tcaatataaa ttgtattaat
    30721 agttgtagct gtatcaccaa ccataccctt attccttcca acgttatcta atttttttag
    30781 tgattctatt atacctgcaa aaagtgaaat agtgtcttta acagcaacag cgtaatatga
    30841 gttgattgaa tatacatagg gattgagttt tttatttttc aaatcaaatt gttgcagacc
    30901 tccattattc atggcaaccc aaatacaatt acgtactttg tcaaacgcta aatcgttaat
    30961 atattcagca cgaaggggac tgttgcgggc atgatagttt tgaaaagtat ttgttttgta
    31021 attataactg caaaaaccat tagatgccgt tccaatccat aataaagagt cgtctgtaac
    31081 ttcaaggcag gtaatattgt tgccactaac agagttttta tcactttgaa atttaaatac
    31141 ctgaaaactt tttccgtcaa aacggttcag cccatctttt gtaccaatcc agatgaatcc
    31201 atatttgtcc tgaatgacac aatttgtttg attgtcggat aagccatcat tagcagtaaa
    31261 ttcattgaga ttgatggtta atgaatccgc caatgcaggt aaagtaaaaa ccagaaatag
    31321 aagtagtgat ttgaaaaatt taatcattcg gttttggcaa ggaattgcct aaaataaata
    31381 aaatcttcta tttaaagaaa acgattggtg aaattaaaag tagaagtggt taaactatgg
    31441 atttgtgtgg tgtaatgatg tgttgttgtt taatcacaca gaagctttaa taattttagt
    31501 cgtgtacaga aaaacgcttt gtaacagaac tgttgctatt agaaattttt aaaacatata
    31561 tgccattagc aagattttgt gtttggacat gaaaaatatt ttctgttcca tttaattctt
    31621 gcgttgaaat tattgctcca ttaatatcat aaacttctgc ggttaaattc ttttcgacat
    31681 tacctgttac tttaaaatag aggttgtttt tattcgtaaa tacagcatag tttatttgta
    31741 gctgttcata gttgtttagt tcaacagcaa ctgaattgct caaatggctt tcaccattaa
    31801 aatcaaattg tcttaaacga tagaaagatt ttcgtgctaa tggttttgaa tctgttgcat
    31861 gatatcgtaa aagctgatta gaaaatcctg cagcctgtgt tttattaatt ggtataaagt
    31921 ttaagtcaac agcagcacgt tctatttcaa agtaatcact atttatttca gatactgttg
    31981 accatcttaa ttcaacttgg tcattattta gttgtgctgt aaaattcagc aactcaacag
    32041 gtaagggatt ttctgaagat gccgatgata aggtaaatgg tccaaataaa ttgtctgctg
    32101 tgcttgtagt aatggtacct gctgacacat ttcctgttgt gctgccattg cctaaattat
    32161 tccatatagt tccaacactg cgccaacgag caactcttaa atcgctgagt aaggttactc
    32221 cacaactgga agcatcccat gataatgtta catttcgact tgaacttcct gcaatttgat
    32281 taagcgtcca gaattcgcac tgactgatgt ggtcaagtgt tggatcaaaa atattactgt
    32341 atgttatttg cggattagca cgcatatatt ctgcaacaaa ctgacttgaa gttaaagaac
    32401 ttagcgaaat atcatctatg gcaaaggatg gatcggtgcc tactccatca tcattgtttt
    32461 cccatttcaa accaattcgc acattagcat tattatttgc acttgccggt agtaccattg
    32521 aaaatgctgt ccattttcct tgcctgaatc cattacatgc tccaccgcaa catgcagtct
    32581 ttggcatatc ggtaatttgt gcccatgcgg caccatcata ataccaaagg gtagcattgt
    32641 caattgtacc atcgccaaat tcaatgtaat taaaactgat gctaatgtta ctttttcctg
    32701 tgcaattgat tacaggcgat tgtgccattc tgtttgtttg ggggcagtat aaaagtccac
    32761 ataacccacc tgcatcatat gcagctccac ctgttggatt aaatacacta ctaacatgca
    32821 tggtattgtt tccattattt gcaactccac aaccacctgc agcaa
//
LOCUS       DAOOOI010000014        31442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_250_length_31442_cov_13.817791, whole genome shotgun sequence.
ACCESSION   DAOOOI010000014 DAOOOI010000000
VERSION     DAOOOI010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 31442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31442
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_250_length_31442_cov_13.817791"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PKX59_03670"
     CDS             <1..350
                     /locus_tag="PKX59_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOZ89998.1"
                     /translation="LIHLFFMNQEKKVIVTPIDFSEQSLIALSQTYNLARLTGSHIRL
                     LHVIDQDFISHLANSIFSKENYADQVRNDIDLMSWQLKCIMKKEFRPLLTSEQEKFMM
                     KLYLKPMTLMLHL"
     gene            248..901
                     /locus_tag="PKX59_03675"
     CDS             248..901
                     /locus_tag="PKX59_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOZ89999.1"
                     /translation="MHNEERVSTSTNIRTGKIYDEIVSEANDVDAAFIVMGTQGASTI
                     KKKFLGSNTSRVIKEALCPVITIKGHEHLKGCKDIALPLDLTKETREKVNYAIDMAKL
                     FGAVVHIFSVLETNDEFLVNKLERQLQQVKQFIIESGVECQGELIPQSDSNVSLSIIE
                     YARKKECDLIIIMTQGETNITDLFIGTAAQEVINNSEIPVLCIRPMVRKDTTQYVLS"
     gene            914..1798
                     /locus_tag="PKX59_03680"
     CDS             914..1798
                     /locus_tag="PKX59_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOZ90000.1"
                     /translation="MSNQLFEIKRILIPYDFSETAALSLEHAVFMAKLLKADIYLLHI
                     LESVSFTSAFGAAFGNVDKKLESESNTKLDEIGKQIHMNNGITVKSITEVGRIYRKIV
                     DVAKRENIDIIIMGTHGVSGYKKFNVGTNTSKVVEEAACPVISVQTHSKKIGFKKIVL
                     PIDNSPQSRQKVSYALAVARSYGSHICIAGLIDFSNEDNKRMFKLKVDQVEEWLLQHG
                     VSVEKKFVEGDNLAKMTMQTAEELDADLIVIMTEQEPSITGFFLGTYATQVVNHSKIP
                     VMAVHPSESDGSTITVGY"
     gene            1888..3567
                     /locus_tag="PKX59_03685"
     CDS             1888..3567
                     /locus_tag="PKX59_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOZ90001.1"
                     /translation="MPSFKGSLRFIFKFLLWTAGILIILFLLFLWRIQIPDPSISSKL
                     KPDDLHRIQIGVDSYRVGNNWLRKNQQGIWEMYIEGNDYERGVVYGVLAKELIEKQEE
                     YFVDQINEIIPRKIYLQFIKMFVAWFNRDIYKYIPAENLREIYGISLSFSDKYDYIGP
                     KYYRILNYHAAHDIGHALTDFQLVGCTSFAANDSLTDDHKLLIGRNFDFYMGDDFARN
                     KLLLFVKPDSGFSFVSYSWAGLTGVVSGMNEKGLTVTINAAKSDIPTTAKDPISLLAR
                     EILQHASNIKQAIAIAEKRETFVSESLLIGSAQDSTAALIEKTPTRMDVYHSPGELLV
                     CSNHYQGAELKNDPHNISNIELSDSKFRFDRITELLKENYPLSITSAVSVLRNKFGMH
                     NENIGYGNPKAINQLIAHHSILFKPQSSDFWVSTMPYQEGVYVHYNLTDVLQGKIPDS
                     DSLNIPESDFVKSNDLKKFEAFKIIKQRIYKHNTFGVNLHLTEAEIKQYIDNNPMSYV
                     TFMSLGDYFYHEKNYAMATLYYNEALKREVASKAEEEKIQKMIESCTEKSK"
     gene            3567..4853
                     /locus_tag="PKX59_03690"
     CDS             3567..4853
                     /locus_tag="PKX59_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ90002.1"
                     /translation="MKQLGEIEFAGLDAIRKMQQQKLSELLQYVNAKSAYYKEVFRQH
                     NIKFDSITLDNLNELPFTTKDDLATQNDSFLCVDKSFIADYVTTSGTLSDPVTFYLTS
                     ADLDRLAYNEAISLQCADGSSADIFQMMTTIDKLFMAGLAYFLGIRKLGAASIRVGPG
                     TPQAQWDYIQRFKPTTLIAIPSFIPQLLQYAAERKINFQSTSVKKIVCLGEPVRNADF
                     SLNELGKRITSLWDVKLYSTYASTEMSSAFTECGYGNGGHAHPELLILEVVDDNGLQV
                     RNGELGEVVVTTLGVEGMPLIRFKTGDLCHAHYDLCKCGRYTTRLGPVMGRKQQMIKF
                     KGTTLFPQIIFNVLNMMPQIELFQVEVKRNEFNNDSITILLSKELHSDAFEHEISSLF
                     KSKIRVTPAFRYIDTEELQQKVFKTDKRKSEKIIYI"
     gene            4879..5508
                     /locus_tag="PKX59_03695"
     CDS             4879..5508
                     /locus_tag="PKX59_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90003.1"
                     /translation="MKKTYLTLIFSFIMLNAFSQEKMNELFKNNVKLIFMGYDFSQAR
                     FIGTDGFTDPAAIKDKFLSAWNNTFVAEYEKFSLQSAFKLTNDKYETNVDVVQDINSK
                     INVHERVMNGSYSIGEDDVKKAVSHYKSKEKDAIGLVYVVESFNKTLEKAVIWVTFVD
                     LSNNKMLYTEKVEEKAGGFGLKNYWIAPATKLKKEIESKKYKIWKNKFK"
     gene            5513..6238
                     /locus_tag="PKX59_03700"
     CDS             5513..6238
                     /locus_tag="PKX59_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOZ90004.1"
                     /translation="MNKIFRFHLSRHILWFCLLSFFLSSCRGIFLSKDKAYSKAIATK
                     PYDAIIVPGFPYFEGQPWPPVVQMRMIWAKFLYQNGYTKNIIFSGNAVYSPFVEGKTM
                     KAFAIAMGIPEEHIYVEDKAEHSVENVYYSYRLAKKLGFKKIALATDPVQTRSMVSFI
                     HRFSLPISYLPIMYKTLRLLPHSEPDIDGKLQRVENFISLPEKETFKQRFAGTLGKNI
                     KWHAEDLKKKRLRKKYSHKIVQE"
     gene            6243..7331
                     /locus_tag="PKX59_03705"
     CDS             6243..7331
                     /locus_tag="PKX59_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017045151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOZ90005.1"
                     /translation="MGSAENTMTEKRELSAFEAKNEAQKLAFAPVVFQVVVAMQRLGI
                     LPLICKNRKGITVKEISEKTNVSIYGVKVLLEMAANADIVKYLDDSTVTVTMLGYFLN
                     SDEMTRVNINFTQDVCYDGFKFLTESIQNGKPEGLKTLGNWPTIYPALSSLPEHIKKS
                     WFEFDHFYSDEAFRDALNIVFKENPSTIFDIGGNTGKWAFASCEFNPDVHVKILDLPQ
                     QIAVAKANAEKRNLTERISYFEINLLDKSQKIPKGADVVWMSQFLDCFGEDEIVSILE
                     NVRQAASPHTTVFILEPFIDNQKFEAASYCLTATSLYFTALANGNSKMYSVKAMTGLV
                     EKSGLEVVETFPLIGESFHTILKCKVKV"
     gene            complement(7374..8675)
                     /locus_tag="PKX59_03710"
     CDS             complement(7374..8675)
                     /locus_tag="PKX59_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOZ90006.1"
                     /translation="MKSIKIMGVLLLSIATVALLTSMKKGSGRYKVKTKGEKGCVFRS
                     VFEAEGIDSNFIYSTPVKVKESMTSALEWISKAQLKDGGWGAGSHARQSVFDPHAVSS
                     DPATTAMVAMALLRTGSTPTTGKYATNLSLATEYLLKQIESADAQTLNITNQTATQPQ
                     VKLGQNIDVVLATQYLTNLMDYISNNQTLKTRVSKSLDKCVVKIQHAQSENGSIKGAG
                     WAGVLQSSFATNALESAKAAGANVDEEKLERYKNYQKGNYDAKTNSVATGDGAGVMLY
                     SISGTARSTAREASIAKDKISRAKKEGKLAPTSTVTSESLQAAGMSEADAYKYAAAYE
                     INQAASKQAQRNDVMNGFGSNGGEEFLSYLQTGEGLIISKDKEWHKWYDNVSGRLLSI
                     QNNDGSWNGHHCITSPVFCTATSLLILSVNNDIEKLVSLNK"
     gene            8893..9282
                     /locus_tag="PKX59_03715"
     CDS             8893..9282
                     /locus_tag="PKX59_03715"
                     /inference="COORDINATES: protein motif:HMM:NF040422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HOZ90007.1"
                     /translation="MSRFPCFGRCPYYDASLYKSGYAVINRKGFMDSLGIYATQIDKQ
                     ELNNFVEDAKKAGLIAMPDSFYNPGIADYPATIVTVNLNGKRKRIFDGVPESPSSLKD
                     FEEKVHLFFTAPNRQWKLIQKPAQQQD"
     gene            9483..12794
                     /gene="secA"
                     /locus_tag="PKX59_03720"
     CDS             9483..12794
                     /gene="secA"
                     /locus_tag="PKX59_03720"
                     /inference="COORDINATES: protein motif:HMM:NF009536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOZ90008.1"
                     /translation="MIDFINKTISKIFGSKAESDYKALSPVIEAVNAVAENMSSLSND
                     ELRNKTDEFRTRIQDYLSEIDTELTQLNEEAEADPEMDANQKEEIYNRIDALKKERNK
                     KTEEVLNEIQPEAFALVREVAKRFTENAELAVEANDNDKNLVTKRDGIRIEDGKAIYS
                     NTWTAAGSKVLWNMIHYDVQLIGGSVLHQGKIAEMATGEGKTLVATLPIYLNALAGAG
                     VHVVTVNDYLAKRDAEWNGPLFEFLGLSVDCIDKHQPNTEERRKAYLADITYGTNNEF
                     GFDYLRDNMTRHAHEMVQRPHHFAIVDEVDSVLVDDARTPLIISGPTPKGDTQEYFAL
                     KPKVEILFNAQRNYINTVIADAKKKLGENKPDEAGMPLIRAYRGLPKNRALVKFLSEQ
                     GVRALLQKTENHYMQDQSKEMHKVDAELFFVIDEKNNTVELTEKGIELITANNEDTHF
                     FVMPDVGASIAEIEKSNGTNEEKLERKEALMRDFSVKSERIHTINQLLKAYTLFEKDD
                     EYIIADGKVKIVDEQTGRVLEGRRYSDGLHQAIEAKEGVKIEAATQTYATVTLQNYFR
                     MYHKLAGMTGTAETEAGEFWQIYKLDVVVIPTNKKIIRDDREDLVYKTKREKYNAVIE
                     EIAKLTAAGRPVLVGTTSVEISELLSRMLKQRNIKHNVLNAKLHKKEADIVAEAGQAG
                     TVTIATNMAGRGTDIKLGQGVKEAGGLAIIGTERHDSRRVDRQLRGRSGRQGDPGSSQ
                     FFVSLEDDLMRLFGSERIAKLMDRMGIEEGEVIQHSMITKSIERAQKKVEENNFGIRK
                     RLLEYDDVMNAQREVIYKKRRHALFGERLALDIMNMQYDVCESIISSYNQERDFDALQ
                     LDLIRYLSIEAPIDAKAFLQIKLEDLTQMVFEKANGHYRQKSNLIAEKAFPVIENIYT
                     NQGQTIVDVVVPFSDGTKAMNVIAGLKKSYDSKCKELISALEKSSVLNFIDEAWTEHL
                     REMDDLRQSVQNAVYEQKDPLLIYKFESFELFKQMIDRVNKEIVSFLIKSYIPVQNQE
                     NVREAKVQQQRTDMSKMQTSHNESDAGGQSRPQGPPPPPKQQPAKAEIKVGRNDPCPC
                     GSGKKFKNCHGAGVD"
     gene            12887..13909
                     /gene="pheS"
                     /locus_tag="PKX59_03725"
     CDS             12887..13909
                     /gene="pheS"
                     /locus_tag="PKX59_03725"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOZ90009.1"
                     /translation="MSQELIEQHIQEAETFSIQSPADLENFRLKYLSKKGVLADMFAL
                     MKNIEPAERKNYGLQVNLLKQKTEAIFESFKEKFENVTTDEKKIDITLPGEPMSEGAL
                     HPLSIIRNRIIEIFSAVGFTVSDGPEIEDDWHNFTALNFPEEHPARDMQDTFFIRKNP
                     DLALRTHTSSVQVRIMEKHKPPLRTISPGRVYRNEAISARAHCFFHQVEGLYIDKDVS
                     FADLKQTLLYFAREMFGADTEIRLRPSYFPFTEPSAEMDISCNFCKGEGCNICKYTGW
                     VEILGCGMIDPAVLDNCKIDSNVYSGFAFGMGIERITMLVYNINDLRIFSENDVRFLK
                     QFSGIG"
     gene            complement(14485..14775)
                     /locus_tag="PKX59_03730"
                     /pseudo
     CDS             complement(14485..14775)
                     /locus_tag="PKX59_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(14861..15727)
                     /locus_tag="PKX59_03735"
     CDS             complement(14861..15727)
                     /locus_tag="PKX59_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOZ90010.1"
                     /translation="MKISIRFFNDREVRAVWDDANAKWWFSVLDIVGVLNNESDYSKV
                     RNYWKYLKAKLKKENNQLVSAATQFKFLAPDGKKRMADMLDSDGVTALAKNFPNNRAM
                     KFLDWFLYSDTSIDGQSKKKAYTLFESNLINEFEVGTTKGLQQIHAYLFGGLYDFAGQ
                     IREKSISKGGYQFVYAQHLKATLQQIDAMPQKALDEIIIKYTEMNKAHLFMEGNGRST
                     RIWLDLMLKKQLKKCVDWSKISKENYMNAMIISTVDSSVLIQVIKPALTNKINDREMF
                     MKGIDYSYYYEE"
     gene            complement(15737..15976)
                     /locus_tag="PKX59_03740"
                     /pseudo
     CDS             complement(15737..15976)
                     /locus_tag="PKX59_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
     gene            complement(16025..20368)
                     /locus_tag="PKX59_03745"
     CDS             complement(16025..20368)
                     /locus_tag="PKX59_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ90011.1"
                     /translation="MKRLFFFILFIGIISNVSAQITASTTSGCVPLVNVNFTSPAGLT
                     NIDWDFGDMTSSNLPNPVHTFFTAGSFTVVCTGLLSGNAFIDSIKINVFRKPFTSLTA
                     TPPLSGCVPLTVHFNDSSVGGGGTAIVSRQWAFGDGATNVGNNSSPTNIYSLAGSFTV
                     SLKVTDANGCDTSISVPNYINTSIQPNAVITTVPSPPIGCTPPFTVAFSASASTSNST
                     TGTGLTYFWNFGGGNTSTVVTPPSINYPTSGTYNISLTVTDNNNCPKTVTTQVVINPP
                     LASFFAVGAVNDTVCPTVTFRNQSIGTNPFYNYGDGTSGVDSIHTYAPGTYQVTLQVL
                     NGICTDDTTITIHVENVVANFTSTPSYSCSWPFTVQYTNITSGAGTYQWIFGDGTTSN
                     LANPSHTFYRPDTNQYTIYDYYYYTDQLTFTSVHGCSATVAKQKNDTVFSITARFMPD
                     KSIGCAPMTVTFSDSSRSRETIVNYTYLFGDGTQTTGSNGVVTHTYNTPGIYYVRLVV
                     TNSAGCKDTSFIIPIYVGSAVTTSFTVVPTTICAYTPVQFNVLNPDTNNIYHFSGDGG
                     MLSHCYNQTNPIWSFNTQTGQQTITLSTSNNGCISTATQTVTVKGPMAKFYVAGNCNT
                     PFKYSFPATINDATTWNWDFGDGTALINSGNVNPTHTYAATGDYWVKLTSFNTTSGCA
                     PDIDSNIVRVRNISAEFTRDSVACKSYTINVDATNATDAYGSCNEGFLWYWGDGTFPH
                     NSSSPIAAHTYFNSGVFYVKLVTTDINGCVDSVKKKVKVYESNAFFKTNKNYGCLPLT
                     VNFTDSSYADTTIASYSWDFGDGSTSSVKNPSHTYTTAPAGGSWTVTLIVTDTIGCTD
                     VYQKTITASLPDTLFSATKTNICVGEPIVFNPHFTGHSSYSWSFGDNTTSAQVSPSHS
                     YNAAGAYTVTLSVTDSIGCPGSKKITNYINVQAYPKAGFTSSADSMLNKCYPLLVNFY
                     DTSIVSVFGSRKWNLGNSSNIFTTPVAGTIYQTPGIYYIQLIEETTFGCRDTAYDTLR
                     VLGPVGNFNIAPTTICKGQSITFSIKDTADLGAFSWDFGDGMTSPGISPITHTYNINP
                     VSGQTLVGLVMWSPDSACTYTQTNPVFIRKVIANFNINSGDTALCIGEPVTLVNTSLN
                     ASNNYWNFGNGQTFNGATPPPYTINQTGVYTITLAIDNSAWGCKDTIRKKISINNLPT
                     VNAIGGDTCAGKPITLHATGGVSYLWSPATGLSNTTVANPVATPGQSTVYTVLITDVN
                     GCTGVATAPVTIYNPPPQVNVTHLLFVGDSVQLNTGLTNNLIINWNPINGLSCVDCPN
                     PWAQPLVNTLYTATFTDSAGCFSGISRFNIEIDPRITIDVPTAFTPNGDGDNDVIFVN
                     GLGLKRLIEFKIYNRWGQLIFESNDLNKGWDGYFKGQLQNVETYVYTAKAEGWLNGKI
                     VSKKGTFNLLR"
     gene            20509..21360
                     /locus_tag="PKX59_03750"
     CDS             20509..21360
                     /locus_tag="PKX59_03750"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HOZ90012.1"
                     /translation="MKAATLIYVILFSCLFAVSDSNAQGLCGTVIDTAKMQAEAAANL
                     NSSQNQRGLFFPVNCLNKTLSITAYIFRDSLAADGITQASILSALQILNNDFAPMCLS
                     FSICEFVYVNNYNYNDFYALKHENEVYNLYYTPNTINVYFSNTLYDNTNSSVCGYAHF
                     PGGRDMIFLNKSCVGDGKTFSHEMGHFFGLYHTFETANGVELINGTNCLVAGDLICDT
                     PADPNGLNGADCQMLPYLPDPSGNWYVPQIGNIMSYYSPGCKCGFTTQQFNWMIQQYL
                     TNRNYLW"
     gene            21394..24141
                     /locus_tag="PKX59_03755"
     CDS             21394..24141
                     /locus_tag="PKX59_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019940250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOZ90013.1"
                     /translation="MKSRKTNQFILIAAITALLSANVFAQDKAKSTTTPVKVTSVEGI
                     TEYKLSNGLRVLLFPDPSKSTITVNITYLVGSRMEGYGESGMAHLLEHMVFKGSTNHT
                     NIPQELTAHGARPNGTTWYDRTNYFETFNATEENLRWALSLESDRMVNSFINKKDLES
                     EFSVVRNEFERGENSPSSVLEERVMSTAYLWHNYGKSTIGSKEDIERVPIENLKAFYH
                     KYYQPDNAVLTIAGKIDEQKTLQLVSEYFSPIPKPERKLQETFTVEPAQDGERNVVLR
                     RVGDVQVASCGYHIPAGAHADYAAIEVLIEALTNEPSGRLYKALVETKKATSTSGYSF
                     QLKDPGYAYFYAEVLKDKPLDTVMNTMKGIFDNLSKNPITNEEAERGKNAILKQLTLM
                     FNNSDRVGLSLSEFIAQGDWRLYFIYRDALEKVTTADVNRVAAEYFKPSNRTTGLFIP
                     DATPDRAVIPASPDVNALVKDYKGKKALAEAEAFDPSPTNISNRIKQATISGGAKYTL
                     LKKTTRGGNVSVNITLRMGNAAVMQNKGTAASFTASMLRRGTKNKSFQQINDEIDKLK
                     SNVGINGNGQTVNVSVTTTKENLSGILNLIGEIIKQPAFAQAEFDKLKEEQISDLESS
                     RSEPQAIAFRVYSKTLNPYPKDDFRYEMSFDEEKEALNKLTLADVKNFYERFYTTSNA
                     TAAIVGDFDEPSLIISLNNLLDKWGSPVLYERAKEPYFDVTPVNEKINTPDKANALMV
                     AGMNLPLKDDNADYPALMVGNYMLGGGFLNSRLATRIRQKEGISYGVGSQLQASSLDE
                     SGGFLSYAIYNPDNSDKLIAAWKEEVQKMLNEGFTNAELTDAVKGLIQARSVSRSQDR
                     ELCSKLNSYTYLGRSIKWDEEFENKIKALKVEDVNAAMKKWINPNKVSFVQAGDFERK
                     K"
     gene            24370..29484
                     /locus_tag="PKX59_03760"
     CDS             24370..29484
                     /locus_tag="PKX59_03760"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ90014.1"
                     /translation="MKKIFGFIVVFLTLSINNLLYGQQPSSCTNLDFELGNFVGWTGK
                     TGSCCPISMFSNGIVTGQHTIMSGTGFDPHSNNQIPVVAPGGTYSARLGNDQAGSQAE
                     QLSYSFQVTPQTELFIYRYAVVLEDPVHDPTEQPRFEIRVFDQAGNPVGCGTYNVVSG
                     AGIPGFQTWLDPNIGSDIHFKTWTTVGIELSSYMGQTVTIEFSTGDCSLGGHFGYAYV
                     DAYCSSMTVTSDYCPGSSTALLIAPPGFASYLWSNGATTQTITINNPVIGATFSVQMT
                     SVTGCVVTLNQVIAVTQLFANFNFNPGCESGTQFFDSSYVLSGSPVNQWLWDFGDGTT
                     STMQNPWHQFPASGNYNVTLTATNAGGCTKSITKSISILPSPVSDFNFTPACPGQAIS
                     FNQNASFPSGTITSYEWLFGDGLPSSAVPDPTHVYSGMGPYDVSLIVTGNNGCVDTIV
                     KPVAPMPDPVAGFTFSVQCMNNTVTFTDVSQLTGGTITAWQWDFGDGSPLDNTANPVH
                     VYNGGGNYAVQLIITGSNGCTDTITKGVAMASLPTAAFTNSGACESQFTAFNDASSLT
                     GGSISSWLWYFGDGTNSTLQNPFHQYNGTGSFNISLVVLGDNGCRDSMARSITVKPSP
                     VASFTSLPVCPGQQAQFNSTAVAPAGETISSWIWNFGDGSAQTAGQNVNHTFTGAGTY
                     AVMSIVGASNGCIDSVVNNISTSITPVAKFSAPSGCALVPLSFSSTSTISSGTITGWD
                     WDFGDGTAHGTTAATTHAYAAPGSYNTYLTVTSNNGCTHTVLHEVSVSGKPVPSFTNS
                     SSCVNDSINLNSVSTSANGSINYLKWDFGDGTFLTGTDSVFHVYADTGFVQIGLIVGN
                     TLGCSDTVYRQIYIKPLPVAGFRWDEMCQSAPGSFYDTSVLINGNISGWLWDFGSGTT
                     NTANPQFAFSNYGVQPVTLTVTGSNNCKATISRNITVRPKPLAQFVSSSACVNAAETF
                     QNTSLISSGNIVSWQWNFGDGHSSISQHGQNTFTTDGYYQVQLFAISDFACADTALDT
                     IRVFPLPVANFFAPHVCLGNATVFNDLSQPISDSLITWSWNFGDGSTSTMPTPSHIFA
                     QDTIYPVSLTVQNEGFCSKTITKNVIIRPLPEVGFTFANPCVGSPVQFQDTSALSGYN
                     INWWQWNFGDSATASIASPVHTYSLSGNIFVKLIVRATNGCSDSVSVPVLVNHLPDAV
                     FSVSDICEQAVLTPNNQSALSGGNITNTLWQWGDNTSDSIFSPTHFYQSDGNFNINLE
                     VTGSNGCKNDTTIAIVIHDKPIASFTHSNACQLAPVAFYDQSSTVNDSLQIWQWQFGD
                     GDQSNLQSPVHDFGAYGVYYTELIVTNSFGCRDTVADSVTVAPKPQAAFSSVNVCKDQ
                     PMIFNNSSSVVAGSIVSNQWSFGDGSSAAVANPTYTYLSTGDYEITLVVTTANQCVDS
                     IKHIYRVYQLPTAGFIADTVEGCQPLLVLFSDSSVANEGLITMWNWNFDNGASDTLTN
                     PAAIVYDQAGYFSPVLYIETNLGCRDTFLRQDYIHVFAKPEAAFIAEPLSNTILHPLI
                     EIKDQSWNTVSWFYDFGDTTNTTEQNPSHWYKQPEHYTIIQYVESADGCKDTALISIE
                     IKNDFTFYMPNSFTPNQDGFNETWNAFGIGITEFNVQVFNRWGQLLFTSNDLSKGWDG
                     TYSGNKVQQGVYVYKATVRDLFNKQHFYVGNVNVIR"
     gene            29497..29787
                     /locus_tag="PKX59_03765"
     CDS             29497..29787
                     /locus_tag="PKX59_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HOZ90015.1"
                     /translation="MNFRIKENALIAWIAARFLGYKTMAIVIGKTIYLHGASVKTFQS
                     DSKWMKHELKHIEQFKRYGFLRFIFLYIIESIRHGYINNRFEIECREAENQD"
     gene            complement(29812..30690)
                     /locus_tag="PKX59_03770"
     CDS             complement(29812..30690)
                     /locus_tag="PKX59_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOZ90016.1"
                     /translation="MEKRSVEIVVSPREPHFVGDGFRVHNFIPSGYRLDMQRMNPFIM
                     LDYNSKFHFDPSDTPKGVGVHPHRGFETVTIAYKGKVEHHDSSGGGGVIGEGDVQWMT
                     AASGVLHKEFHEKEWSKTGGEFQMVQLWVNLPAKDKMSKPKYQALENSKINRFKSDDK
                     KTEIEIIAGEYKGVKGSASTFTPLHLYNVKMAKDAKTEMNLPKNFNTALLVIEGSITI
                     NGTEKVDTNHFALMDNDGESFTIDSNENAVYLVLSGEPINEPIAAHGPFVMNTRQELV
                     QAFEDFNQGRFGTLQE"
     gene            30875..31267
                     /locus_tag="PKX59_03775"
     CDS             30875..31267
                     /locus_tag="PKX59_03775"
                     /inference="COORDINATES: protein motif:HMM:NF022264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HOZ90017.1"
                     /translation="MKQRISVVMGLLIMVTMVSLSFKPDNWRILGTKTVDYQLDKDVL
                     DVQLRDGVFQKLKFVVRGGALNMHKVIVHFENGGEQEIDMRHSFDKNSQSRVIDLKGN
                     KRIIEKIVFWYDTKNASNRKAKVTVLGR"
     gene            complement(31280..>31442)
                     /locus_tag="PKX59_03780"
     CDS             complement(31280..>31442)
                     /locus_tag="PKX59_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HOZ90018.1"
                     /translation="QAKINPNASKITGTICGYKIEEIENPFMKKVRYLDKLVDELAKG
                     HPLTKILRS"
BASE COUNT         9868 a         5679 c         6184 g         9711 t
ORIGIN      
        1 agttaataca cctgtttttt atgaaccaag agaagaaggt tatagttacc ccgatagatt
       61 tttcagaaca atcactcatt gccctttcac agacttataa tcttgcacga ctgaccggca
      121 gccatatcag gcttttacat gtaattgatc aggattttat ttcccattta gccaacagta
      181 ttttttctaa agaaaactat gccgatcagg ttcgaaatga tatagacttg atgagttggc
      241 aactaaagtg cataatgaag aaagagtttc gacctctact aacatcagaa caggaaaaat
      301 ttatgatgaa attgtatctg aagccaatga cgttgatgct gcatttatag tgatgggaac
      361 acaaggggca agcacaataa agaagaaatt tcttggctct aatacttcaa gagtaattaa
      421 agaagcttta tgccccgtca ttaccataaa aggtcatgag cacctcaagg ggtgtaaaga
      481 tattgctctt ccattggatc ttacaaaaga aacacgcgaa aaggtgaatt atgccattga
      541 catggctaaa ctttttggtg ctgtagtgca cattttttca gtgctcgaaa ccaatgatga
      601 gtttttagtc aataaactgg aacgacaatt gcagcaggta aagcagttca ttattgaatc
      661 cggagtagaa tgtcaaggtg aattgattcc acaaagtgat tcaaatgtat cattaagtat
      721 aatcgaatac gcaagaaaga aagagtgtga tttaattatt atcatgacac agggagaaac
      781 caatattaca gatttattta tcggcacagc agcacaggaa gttatcaata actcagagat
      841 tcctgtttta tgtatccgac caatggttag aaaagacact acacaatacg tactatcata
      901 aatccatttt cctatgagta atcaattatt tgaaatcaag agaattttaa ttccttatga
      961 tttttcagaa acagcagcgt tgagcttgga gcatgcagtt ttcatggcca aattattaaa
     1021 ggcagatatt tatctgctac atattcttga atctgtatct tttacttcgg catttggtgc
     1081 agcgtttgga aatgtagata aaaagttaga gtctgaatcg aatactaaac tcgatgaaat
     1141 cggcaaacaa atccacatga acaacggaat aaccgtaaaa tctattactg aggttggaag
     1201 gatttatcgc aaaattgttg atgttgccaa acgtgagaac attgacatta taattatggg
     1261 aacacatggt gtttcaggat ataagaagtt taatgttgga actaacacca gtaaggtagt
     1321 tgaagaagct gcatgtccgg tgatttctgt acagacacac agcaaaaaaa tcggctttaa
     1381 gaaaattgtt ttaccaattg ataattcacc gcagtcacgt cagaaggtaa gctatgcatt
     1441 agcagtagcc cgctcttatg gctcgcatat ttgtattgcc ggattaatag acttcagcaa
     1501 tgaagacaat aagcggatgt ttaaacttaa agttgatcag gttgaggaat ggcttctaca
     1561 acatggtgta agtgttgaga aaaaatttgt tgaaggtgat aacctagcaa aaatgactat
     1621 gcagacagca gaagagttgg atgccgattt aattgtaata atgacagaac aagaacctag
     1681 tattacagga ttttttctgg gcacctatgc aacacaggtt gttaaccaca gcaaaattcc
     1741 tgttatggca gtgcatcctt cagaatcgga cggcagtaca attactgtag gatattaaaa
     1801 aacaaatttc atgatttaca catggggcgc ttgaagagcg ccccatgttt ttattacctt
     1861 cgccaatcag aattttaatt actataaatg ccatcattta aaggtagtct gcgttttatt
     1921 tttaagtttt tactttggac tgcaggaata cttattatat tatttctttt gtttttatgg
     1981 aggattcaga ttcccgatcc ttctataagt agtaaactta aaccagatga ccttcatcgc
     2041 attcaaatag gagttgacag ctatcgtgtt ggcaacaact ggttgcgcaa aaatcagcaa
     2101 ggcatctggg aaatgtatat tgaaggtaat gactatgaaa gaggtgtagt ttatggtgtg
     2161 cttgcaaaag agttaattga aaaacaagaa gaatattttg ttgatcagat caatgaaata
     2221 attccacgta aaatctatct tcagtttatc aagatgtttg tagcatggtt caatcgcgat
     2281 atttacaaat atattccggc tgaaaatctg cgtgagattt atggtatttc actttctttt
     2341 tctgacaagt acgattacat aggacctaaa tattatcgca tcctcaatta tcatgcagca
     2401 cacgacattg gtcatgctct taccgatttt caattggtag gctgtacttc atttgctgcc
     2461 aacgacagtc ttactgacga tcataaatta ttaatcggca gaaattttga tttttacatg
     2521 ggggacgatt ttgcacgaaa caaactctta ctttttgtaa agcctgatag tggtttttct
     2581 tttgtttctt attcgtgggc aggacttaca ggagttgttt ccggtatgaa tgaaaaagga
     2641 cttactgtta ccatcaatgc tgcaaagtct gatatcccaa caactgcaaa agatcctatt
     2701 tcacttttag cacgcgaaat tttacaacat gcatcaaaca taaagcaagc gattgctatt
     2761 gcagaaaagc gcgaaacttt tgtttcagaa tcgttgctta ttggttcagc gcaggatagt
     2821 acggctgcct tgatagaaaa aacaccaaca agaatggatg tatatcattc acccggtgaa
     2881 ttattggttt gttccaatca ctatcagggt gctgaattga aaaatgatcc acacaacatt
     2941 tcaaatattg aacttagcga cagtaaattc cgctttgaca gaataacaga attgctaaag
     3001 gagaattatc cattaagtat aacatcagca gtttctgttc tgcgaaataa atttggaatg
     3061 cacaatgaaa atattggcta cggtaaccca aaggcaatta atcagcttat agcacatcac
     3121 agtattttat tcaaaccaca aagcagcgat ttttgggttt ctacaatgcc ttatcaggaa
     3181 ggtgtttatg ttcactataa tttaactgat gttttacagg gtaaaattcc cgattctgac
     3241 tctctgaata ttccggaaag tgattttgta aagtctaacg atttaaagaa gtttgaagct
     3301 tttaaaatta tcaaacaacg aatctacaaa cacaacacat ttggtgtaaa tctgcatctg
     3361 actgaagccg aaataaaaca gtatatcgat aataacccta tgagttacgt tacctttatg
     3421 agcttgggtg attattttta tcatgagaaa aattatgcaa tggccacact ttattacaat
     3481 gaagcattaa aacgtgaagt agcatcaaag gcagaggagg aaaaaattca aaagatgata
     3541 gaatcctgta ctgaaaaatc aaaataatga aacagcttgg tgaaatagaa tttgcaggtc
     3601 ttgatgccat tcgaaaaatg cagcaacaaa agctttctga actgctgcaa tacgttaatg
     3661 ccaaatcagc ttattataaa gaagtcttca ggcaacacaa cattaagttt gacagcatta
     3721 ctctcgacaa tctgaacgaa ttgccattta caactaagga tgacctggca acacaaaatg
     3781 attctttttt atgtgttgat aaaagtttta ttgcagatta tgtgactaca tcaggtactt
     3841 tgagcgatcc tgttacattc tatctgacat cggcagactt agacagattg gcatataacg
     3901 aggccatatc cttgcaatgt gctgatggca gcagtgcaga tatttttcag atgatgacta
     3961 ccattgacaa attgtttatg gcaggattag cctatttttt aggtatcaga aagttgggtg
     4021 ctgccagtat cagagtaggg ccgggaacac ctcaggcgca gtgggattat attcaacgtt
     4081 tcaagccaac aacattaata gccataccat catttattcc acaactgttg caatatgcag
     4141 ctgaaagaaa aattaatttt caatcaacct cggtaaaaaa aatagtttgt cttggagagc
     4201 ctgttcgtaa tgcagatttt tcattgaatg aactaggaaa aagaatcact tcgctttggg
     4261 atgtgaaatt gtattcaacc tatgcatcaa cagagatgag ttctgcattt acagaatgtg
     4321 gctatggcaa tggaggtcat gcacatcctg agttattaat tcttgaagtt gttgacgata
     4381 atggtcttca ggtacgtaat ggcgaattgg gcgaagttgt tgtcacaacc cttggtgtag
     4441 aaggtatgcc tttaatcaga tttaagaccg gtgatttatg ccacgctcat tatgatctgt
     4501 gtaaatgtgg ccgatatact acccgcttag gtcctgttat gggtagaaag caacagatga
     4561 ttaagtttaa aggcactaca ttgtttccac aaataatttt taatgtactc aatatgatgc
     4621 cgcaaattga attgtttcag gttgaagtca aacgaaatga atttaataat gattcaataa
     4681 caattctttt gtcaaaagag ttacattctg atgcatttga acatgaaatt tcttcattat
     4741 tcaagtccaa gataagagta actccggcat tcaggtatat tgatactgaa gagttacagc
     4801 aaaaagtttt taagacagat aaacgtaaat ccgaaaaaat aatttacatt tgaaaaatta
     4861 attttaataa aatagaaaat gaaaaagacc taccttactt taatcttttc attcatcatg
     4921 ctcaatgcat tttcacagga aaaaatgaat gaactcttta agaacaatgt gaaactcatt
     4981 tttatgggtt atgacttttc acaagcgcga tttatcggaa cagacggatt taccgaccct
     5041 gctgccatca aagacaagtt tctttcggca tggaataata cttttgttgc agaatatgaa
     5101 aaattcagtt tgcaaagtgc ctttaagctt actaacgata agtacgaaac caatgttgat
     5161 gtagttcagg atatcaacag taaaattaat gtacacgaaa gagtgatgaa tggaagctat
     5221 tcaattggag aagatgatgt aaaaaaagca gtttcacatt ataaatcgaa ggagaaagat
     5281 gcaattggtt tggtttatgt agttgaaagt ttcaataaga ctttagagaa agctgttatc
     5341 tgggtaacat ttgttgacct gtcaaataac aaaatgcttt acacagaaaa ggtagaagag
     5401 aaagcaggtg gttttggatt gaagaattac tggattgcgc ctgcaaccaa gcttaaaaaa
     5461 gaaattgagt ctaagaaata caaaatctgg aagaataaat tcaagtaaat aggtgaataa
     5521 aattttccgc tttcatttaa gcaggcatat tctatggttt tgcctgcttt ctttttttct
     5581 ttcttcttgc agaggaattt ttttatctaa ggataaagca tacagcaaag caattgcaac
     5641 aaaaccttat gatgcaatta ttgttcccgg gtttccatat tttgaaggtc agccctggcc
     5701 acctgtagta caaatgagaa tgatttgggc aaaatttcta tatcagaatg gctacactaa
     5761 aaatattatt ttttcgggca atgctgttta ctcacccttt gtcgaaggaa agacaatgaa
     5821 agcatttgct atagccatgg gcatacccga agaacatatc tatgtagaag acaaagcaga
     5881 gcacagcgta gaaaatgtgt attattcata tcgtttggca aagaagttag gatttaaaaa
     5941 aattgccttg gcaacagacc ccgttcaaac ccgttcaatg gtaagtttta tccatcgctt
     6001 tagtctgcca atctcctatc tgcccattat gtacaaaaca cttcgtttac tgccgcatag
     6061 tgagcctgat attgatggaa aattgcagag agtagaaaat tttatttctt tgcctgagaa
     6121 agaaacgttt aaacaacgct ttgcaggaac tttaggtaaa aacataaaat ggcatgccga
     6181 agacctgaag aaaaaaagat tgcgtaaaaa atattcacac aaaatagttc aggaatagaa
     6241 agatgggttc agcagaaaat acaatgacag aaaaaagaga attatcagca tttgaagcaa
     6301 aaaacgaggc gcaaaaatta gcgtttgctc cggtagtttt tcaggtagtg gttgccatgc
     6361 aacgcttagg cattttacca ttgatttgta aaaatagaaa aggaattact gtaaaagaga
     6421 taagtgaaaa gactaacgta agtatttatg gagtaaaagt tttgctcgaa atggcagcca
     6481 atgcagatat tgtaaaatat ttagacgaca gtactgtaac agttactatg ctcggctatt
     6541 ttttgaattc tgatgaaatg actcgtgtta acataaactt tacacaggat gtttgttatg
     6601 atggattcaa atttctgaca gagtctattc aaaatggtaa acccgaagga ttaaaaacgc
     6661 tcggcaactg gccaactatt tatcctgctt tatcatcatt gccagaacat attaaaaaat
     6721 cgtggtttga gtttgatcat ttttatagcg atgaagcatt ccgtgatgcg ctcaacattg
     6781 tgtttaaaga aaatccatca actatttttg atattggtgg caataccggc aaatgggcat
     6841 ttgcttcctg tgaattcaat cctgatgtgc atgtaaaaat tcttgacctg ccacaacaaa
     6901 ttgcagtggc aaaagccaat gcagagaaaa gaaatctaac agagcgcatt tcatattttg
     6961 aaatcaacct gcttgataaa agtcagaaaa ttcctaaagg tgccgatgtt gtttggatga
     7021 gtcagttttt agattgtttt ggtgaagatg aaattgtctc tattcttgag aatgtcagac
     7081 aggcagcatc acctcacact actgttttta ttctcgaacc attcattgat aatcaaaagt
     7141 ttgaagccgc ttcttattgc cttactgcaa catcactgta ttttacagcg cttgcaaacg
     7201 gcaacagtaa aatgtacagt gtaaaagcaa tgacaggttt ggtggaaaag tcagggcttg
     7261 aagtggttga gacatttcca ttaattggcg aaagttttca tactatactc aagtgtaaag
     7321 taaaagttta aaaaattacg ggagtgctgc catgcagcac tcccgtaatt cttttattta
     7381 ttcagcgaaa ccaatttttc aatatcatta ttaacagata gaataagtaa tgaagttgct
     7441 gtacaaaaaa ccggacttgt aatacagtga tgtccattcc aactgccatc attattctgg
     7501 atgctcaaca atcttccgct aacattatca taccatttat gccactcttt atccttactg
     7561 attattaaac cttcaccggt ttgcagatat gaaagaaact cttcaccacc attgctgcca
     7621 aatccattca tcacatcatt acgctgtgcc tgcttacttg cagcctgatt aatttcgtaa
     7681 gcagcagcat atttgtaagc atcagcctca ctcatgccgg cagcctgcag actttcgctt
     7741 gttacagttg aagtaggcgc tagcttgcct tctttttttg ctctcgatat tttatctttt
     7801 gcaatacttg cctcgcgtgc tgtgctgcga gcagtgcccg aaatactgta taacattact
     7861 ccggcaccat cacctgtagc tacggaattt gtctttgcat catagttacc tttctgataa
     7921 tttttatatc gctcaagttt ttcttcgtca acatttgctc ctgcggcttt tgcactttct
     7981 aatgcattag ttgcaaacga tgattgtaaa acacctgccc atcctgcacc ttttatactt
     8041 ccattttcgc tctgggcatg ttgtattttt acaacacatt tatcaagact cttacttact
     8101 ctcgttttca atgtctgatt gttagatatg taatccatca gattggtcag gtattgtgtt
     8161 gcaagaacta catctatatt ttgcccaagt ttaacctgtg gttgtgttgc agtttgattg
     8221 gtaatattta aagtttgtgc atctgcagac tctatttgct tcaataaata ttctgttgct
     8281 aaagaaagat tggtagcata ttttcctgtt gtaggtgtgc ttcctgtgcg aagcaaggcc
     8341 atggcaacca tagctgttgt ggcagggtca gaacttacag catgtgggtc aaaaaccgac
     8401 tgtctggcat gtgagcctgc tccccagcca ccatctttta actgtgcttt gctaatccac
     8461 tccaatgcag atgtcataga ttctttaact tttaccggtg ttgaataaat aaaatttgag
     8521 tcaatacctt ctgcttcaaa aacagaacgg aaaacacatc ctttttctcc tttggttttt
     8581 actttgtaac ggccactacc ttttttcatt gatgtaagta atgccactgt tgcgatagag
     8641 agtaatagca ctcccataat ttttatactt ttcatttcct taaattttta ttgattttac
     8701 taaggatgta actacgcaca aaatttcaca catttatttg acaaaggcaa atgttatttt
     8761 cacatttgca gaatgaaaca ggccaattta ttttttgttc ctataacaat actaatttta
     8821 tcagctatct tttcatgaaa cacttcaaaa aaagtttctg aaaataattc agcagatagt
     8881 ttactcatca gcatgagtcg ttttccatgc tttggccgat gcccatatta tgatgcatca
     8941 ttatataaga gcggttatgc agtcatcaac agaaaaggat ttatggatag cctcggtatt
     9001 tatgctacac agattgataa acaggagttg aataattttg ttgaagatgc aaagaaggca
     9061 ggacttattg ctatgcccga tagtttttat aatcccggaa ttgcagatta ccctgctact
     9121 atcgtcaccg taaatttgaa tggtaaaaga aaacgcattt ttgatggtgt gcccgaatct
     9181 ccttcttctt taaaagattt tgaagaaaag gttcatctgt tttttacagc accaaatcgt
     9241 cagtggaaac tgatacaaaa acctgcacaa cagcaggatt agaaagcttt tatgtcatta
     9301 tgtcgccaaa tactttttgg catttctgtt gacaacagtt tgctgatgag ggttctaaat
     9361 tcatgcacca atcatttaaa gcatagtttt taggcaatca tcctttgcaa atctctttga
     9421 attgcagcca ctttacgtta cttttgtcac cttttatttt tagcacatta taatcagcaa
     9481 caatgattga ttttatcaat aaaactattt ccaaaatttt tggcagtaag gccgaaagcg
     9541 attacaaagc actgtcacct gtaattgaag cagtaaatgc agtagccgaa aacatgtcat
     9601 cactcagcaa tgatgaatta cgcaataaaa ccgatgaatt tcgtacgcgc atacaggact
     9661 atctttcaga gattgataca gaacttactc agcttaacga agaagctgaa gccgatcctg
     9721 aaatggatgc caaccaaaaa gaagaaattt acaaccgcat tgatgcttta aagaaagaga
     9781 gaaataagaa gacagaagag gtgctgaacg aaattcagcc tgaagcattt gcacttgtcc
     9841 gcgaagttgc caaacgtttt actgagaatg ccgagctggc agtagaagcc aatgacaatg
     9901 ataaaaatct ggtaactaaa cgcgatggta tcagaataga agatggcaag gcaatataca
     9961 gcaatacctg gacagcagca ggaagtaagg ttctctggaa catgattcat tatgatgtgc
    10021 agcttatagg tggctctgtt ttgcatcagg gaaaaattgc agagatggca accggtgagg
    10081 gaaaaacgct tgttgcaaca ttacccattt atctcaacgc cttggcaggt gcaggtgtgc
    10141 acgttgttac tgttaacgac tatcttgcaa agcgtgatgc tgagtggaat ggtccgttat
    10201 ttgaattttt aggactttct gttgattgta ttgataaaca tcaacccaac actgaagaaa
    10261 gacgtaaggc atatttagct gatattacct acggaactaa taatgaattt ggtttcgatt
    10321 atctgcgtga taatatgacg cgtcatgctc atgaaatggt acaacgacca catcattttg
    10381 caattgtaga tgaggtggat agtgtattgg ttgatgatgc aagaacaccc ttgattattt
    10441 caggtccaac acctaaaggc gacactcagg aatattttgc attgaaaccc aaagttgaaa
    10501 tactgtttaa tgcacaaaga aattatatca acactgtaat tgccgatgct aaaaagaaat
    10561 tgggagaaaa taaacccgat gaagcaggca tgcccttgat aagagcttac agaggtctgc
    10621 ccaaaaacag agcattggta aagtttttaa gtgagcaggg tgtaagagca ttattgcaga
    10681 aaacagaaaa ccactacatg caggatcagt caaaggaaat gcataaagta gatgcagaat
    10741 tgttttttgt gattgacgaa aaaaataata ccgttgaact tacagagaaa ggtattgaac
    10801 tgattactgc caacaacgaa gacacacact tttttgtgat gcctgatgtt ggtgcttcta
    10861 ttgcagaaat tgaaaaatct aatggaacta acgaagaaaa gttagaacgc aaagaagccc
    10921 tgatgcgcga cttctctgta aaatcagagc gtattcacac catcaatcag ttactaaaag
    10981 catatacgct tttcgaaaaa gatgatgagt atattatcgc agatggaaaa gtgaaaattg
    11041 ttgatgagca aacaggacgt gttcttgaag gaagaagata ttccgatggt ttacatcagg
    11101 caattgaagc taaagaaggt gtaaagattg aagctgctac acaaacgtat gcaacggtga
    11161 ccttgcagaa ctatttccgt atgtaccaca aactggctgg tatgacaggt actgcagaaa
    11221 cagaagccgg agaattttgg cagatatata agttggatgt agtagttatt cctaccaaca
    11281 aaaaaattat tcgtgacgac agagaagatt tggtttataa aaccaagcgt gaaaaataca
    11341 atgccgttat tgaagagatt gcaaaactaa cagctgcagg aagaccggtt cttgtaggaa
    11401 caacatcggt tgaaatttct gaattgctta gccgtatgct caagcaaaga aatattaagc
    11461 acaatgtttt aaatgctaag ttacataaga aagaagcgga tattgttgca gaagcagggc
    11521 aggctggaac ggtaactatt gctaccaaca tggccggtcg tggtacagat attaaattag
    11581 gtcagggagt aaaagaagcc ggtggtcttg caattatcgg tacagaacgc catgactcaa
    11641 gacgtgttga cagacagttg cgcggacgtt caggtcgtca gggtgacccg ggttcatcac
    11701 agttttttgt ttcgttagaa gatgatttaa tgcgtttgtt cggcagtgaa agaattgcca
    11761 aactaatgga cagaatgggc attgaagagg gtgaggttat tcagcatagc atgattacca
    11821 aatctattga acgtgcacag aagaaggttg aagagaataa cttcggaata cgtaaacgtt
    11881 tgctcgaata cgatgatgtg atgaatgcac agcgcgaagt tatctataag aaaagacgtc
    11941 atgcattgtt tggagaaagg cttgcactcg acattatgaa catgcaatat gatgtttgcg
    12001 aaagcattat aagcagctat aatcaggagc gcgattttga cgcattacag ttagacctta
    12061 ttcgttatct gtcaattgaa gcacccatag atgcaaaagc atttttgcag ataaaactcg
    12121 aagacctgac acaaatggtt ttcgaaaaag caaacggcca ttaccgacag aaatcaaatc
    12181 ttattgcaga aaaagcattt cctgttattg aaaatattta cactaatcag ggtcagacaa
    12241 ttgtagatgt tgttgtgcca tttagtgatg gtactaaggc aatgaatgta attgcaggac
    12301 taaagaagtc gtacgattca aaatgtaaag agttgatttc tgctctcgaa aaatcatcgg
    12361 tactcaattt tattgacgaa gcatggacag agcatttgcg cgaaatggac gacttgcgtc
    12421 agagtgtaca aaatgcagtt tacgaacaga aagatccttt gttgatatat aagtttgagt
    12481 cgtttgaact tttcaagcag atgattgaca gggtgaataa agaaattgtt tcgtttttga
    12541 ttaaaagcta tatccctgtt caaaatcagg aaaatgtacg tgaagctaaa gtgcagcagc
    12601 aacgtactga tatgagcaaa atgcaaacaa gccacaacga atcagatgca ggagggcaaa
    12661 gccgtccgca aggaccaccg ccaccaccaa agcaacaacc tgcaaaggca gaaataaagg
    12721 tgggacgtaa tgacccttgc ccttgtggta gtggaaagaa atttaaaaac tgtcatggtg
    12781 caggtgttga ttaatacaat agttttattt gtcattattt ctaatagaat attaacatca
    12841 taagcaaaag ttttaatcgt taatttgcat tatcttattt tatcttatgt cgcaagaatt
    12901 aattgagcag catattcagg aggcagagac attttcaatt cagtcacctg ccgatcttga
    12961 aaatttccgt cttaaatatc tttctaagaa aggtgtgttg gcagatatgt ttgcattgat
    13021 gaaaaatatt gaacctgccg aaagaaaaaa ttacgggttg caggttaact tgctcaaaca
    13081 aaagacagaa gccatcttcg aaagttttaa agagaaattt gagaacgtta ctactgacga
    13141 aaagaagata gacattacct tgcccggtga gccaatgagt gaaggtgcat tgcatccgct
    13201 atcaattatc agaaacagaa ttatcgaaat tttcagtgct gttggattca cagtttctga
    13261 tggacctgaa atagaagatg actggcataa ttttacagca ctcaattttc ctgaagagca
    13321 tccggcccgc gacatgcagg atactttttt tatccgcaaa aaccctgacc ttgcactgcg
    13381 tacacataca tcatctgtac aggtgagaat aatggaaaaa cacaaacccc cattacgtac
    13441 aatatcaccg ggaagagttt accgtaatga agcgatttca gcaagggcac attgtttttt
    13501 tcatcaggta gaaggactct acattgacaa agatgtttcg tttgccgacc tgaaacaaac
    13561 attactctat tttgcaagag agatgtttgg tgctgacact gaaattcgtt tacgcccttc
    13621 gtattttccg tttacagagc catcagcaga gatggatata tcctgtaatt tctgtaaggg
    13681 cgaaggctgc aacatctgta aatacacagg ctgggtagaa attcttggct gtggcatgat
    13741 tgatcctgcc gttctcgaca attgtaaaat agacagcaat gtttacagcg gttttgcctt
    13801 cggtatgggt atagaaagaa ttactatgtt ggtttataac atcaacgacc tgcgaatttt
    13861 ttctgaaaat gatgttcgtt ttttaaagca gttcagtggg atagggtagg ggtggtttac
    13921 tttatgcttt cttattcctg tcccaactac aatacttagt gttgtcccga aaactatcgg
    13981 gacgttggct catacaattt tgtttattgg tttctcaata ccttttagta gcgcttccaa
    14041 ctgaccgccc gggcttgcgt aaccgatggt gatttggtct gcaaggcttg tcattagttg
    14101 gttgcgtgtc tgtgcgtttt gactgcttac tcgttttacc gatttgtcaa acggtgtgat
    14161 gattaacagt ctgccttgtt ccaattgctt ttcaaattca ggttccaatt tttcttttag
    14221 acctcttgct aatgctaaaa tgatgggctg tttgcctttg agcagataat gcaaaacatc
    14281 tttttctatt tggctgtgaa aaccactgat aacacacacc tttttctctc tttgctctat
    14341 tgcccagtca ttgcatttta ataccaccga agcaggaact tgtcggctgc ataaaaaagc
    14401 tgttttaggt agctttaaaa ggtcggtatt gccaaggctc tcttttatag cggtggtttc
    14461 ggctccgctc aaccaccgat tggaaaaatg cgtttcattt ttacattctg ctaaaagatg
    14521 tagtttttca ggtttatttt ctattaatgc ttctttcttt tttctactcc agccttgcac
    14581 ttgtttttct ctgtaaaatg ctttgtctat tctttggaat tcttcaacat atactaactc
    14641 caccggcagt cgctttttag tatgattggc tccttcacct gactgatgtt gctgtaatcg
    14701 caatttaagg tttttagtac tacctacata gtaactatca tctgagcatt ttagaatgta
    14761 catgtatcct ttcatggtgt tggtttaata atgttgcctt cggctacgct caggcaacgg
    14821 aatagtagct ggtggttgag cgcagccgaa accaccttta tcattcttca tagtaatatg
    14881 agtaatcaat acctttcata aacatttccc tgtcgttgat tttattggtt agggcgggct
    14941 ttatcacttg tatcaataca ctgctatcaa ccgtgctaat tatcatagca ttcatataat
    15001 tctctttgct tattttactc caatccacac attttttcag ttgtttttta agcatcaaat
    15061 caagccatat tcttgtgctt cgcccgttgc cttccataaa aagatgtgcc ttattcattt
    15121 ctgtatattt aataataatc tcatcaagcg ctttttgtgg catggcatca atttgctgca
    15181 atgtggcttt taaatgctgt gcataaacaa attgatatcc tcctttcgat atactttttt
    15241 ctcttatttg cccagcaaaa tcatataagc caccaaacag gtaagcgtgt atttgttgta
    15301 atcccttagt tgttcccact tcaaattcgt tgattaaatt actttcaaaa agggtgtagg
    15361 cttttttctt gctttgtccg tcaatgctgg tatcgctata aagaaaccag tctaaaaact
    15421 tcatggctct gttgtttgga aaatttttgg cgagagctgt tacgccatca ctatccaaca
    15481 tatcagccat tctctttttg ccatcgggtg caagaaattt gaactgggta gcggcactaa
    15541 ccagttgatt gttttctttc ttcaacttgg ctttaaggta tttccaatag ttacggactt
    15601 tactgtaatc actttcattg tttaatacac ccacaatatc caataccgaa aaccaccact
    15661 tggcgttggc atcatcccaa acagcccgca cctcacggtc gttgaagaag cggatggata
    15721 ttttcatttg tctgaattaa gatttgttgg atttgatgat tgtaggattg cgtttgatgt
    15781 agcccttatt ctcaggatga ttaacattat acaccctttt aaattcaagg ctctttgcac
    15841 caaaattgat aagtaagcct atgggcaaat tataggcctg gtaataattc attgcatgtg
    15901 ccaaatgcac ttcttcaagt tttatcaggg ctttcaactc taccataata ttctcttcaa
    15961 caaacaaatc cctcttgctt tttctaaaat tttgttccta tgtgatatct tattatggtt
    16021 tacatcatct caacaaattg aaagttcctt tcttactcac aattttgcca ttcaaccaac
    16081 cttctgcttt ggcagtgtaa acataggtct ctacattctg caactgacct ttaaaatagc
    16141 catcccagcc tttgtttaag tcgttgcttt caaaaatcaa ctgtccccaa cgattgtaaa
    16201 ttttaaattc tatcagacgt ttcagtccga gtccatttac aaatattaca tcgttatcgc
    16261 catcaccgtt gggtgtaaaa gctgttggaa catcaatagt tattcttgga tctatttcta
    16321 tgttgaatct tgaaatgcct gaaaagcaac ctgcagaatc tgtaaatgtt gcagtgtaaa
    16381 gtgtgtttac caacggctgt gcccatgggt tagggcaatc aacacaactt aatccgttaa
    16441 taggattcca attgatgatt aaattatttg taaggcctgt atttaattga acagagtcgc
    16501 caacaaaaag taaatgcgtt acattgactt gcggaggtgg attgtaaatt gttacaggtg
    16561 cagtggcaac tcctgtacat ccgtttacat cggttatcaa tacggtataa acggtgcttt
    16621 gacccggtgt ggcaacagga ttggcaactg tagtgtttga aagtcctgtt gctggcgacc
    16681 acagataact tacaccacct gttgcatgta aagttattgg tttgcctgca caagtgtcgc
    16741 cacctattgc atttactgtc ggaagattat ttatgcttat tttttttctg atagtgtcct
    16801 tacatcccca cgctgaatta tctattgcta atgtaatggt gtaaacccct gtttgattaa
    16861 tggtgtaagg cggtggtgtt gcaccattaa aagtctgacc attaccaaag ttccagtagt
    16921 tattacttgc atttaaagaa gtattgacta atgtaacagg ttcaccaata cataaagcag
    16981 tgtcgccact gttaatgttg aagtttgcaa taacttttcg aataaacacc ggattggttt
    17041 gtgtgtaagt acaagccgag tcaggcgacc acattaccaa acctacaagc gtttgtccgc
    17101 ttacgggatt aatattgtat gtatgtgtta tgggtgaaat tcccggagag gtcataccat
    17161 caccaaagtc ccacgaaaat gcaccaaggt ctgctgtatc tttaatgctg aaagtaattg
    17221 attgcccttt acaaattgtt gttggtgcaa tgttgaagtt gccaacaggt ccaagtacac
    17281 gaagtgtatc ataagcagtg tctctgcaac caaaggttgt ctcttcaata agttgaatgt
    17341 agtaaatgcc gggcgtctga taaattgttc ctgccacagg tgttgtaaaa atgttactgc
    17401 tgttgccaag attccacttg cggctgccaa aaacactaac aatacttgta tcataaaagt
    17461 taaccaacaa tggataacat ttattgagca tactgtctgc actactggta aatcctgctt
    17521 tgggataggc ttgtacattg atgtagttag taattttttt acttccagga cagccgatgg
    17581 aatcggtaac agataatgtt actgtgtagg cacctgctgc attataacta tgcgatggtg
    17641 atacctgtgc tgatgtggtg ttgtcaccaa agctccacga gtatgatgaa tgtcccgtaa
    17701 aatgtggatt gaagacaata ggttcgccaa cgcaaatatt tgtttttgtt gcagagaata
    17761 acgtatccgg caatgacgca gtaattgtct tttggtaaac atcggtgcag ccaatagtat
    17821 cagtaacaat taatgttact gtccaacttc cacctgcagg tgcagtagtg taggtgtgac
    17881 ttgggttttt gacagaagaa gtgcttccat caccaaagtc ccatgagtaa gaagctatgg
    17941 ttgtatcggc ataactactg tcagtaaaat tcaccgttag tggcagacag ccataatttt
    18001 tattggtttt aaaaaaagca ttggattcat aaactttcac ttttttcttt actgaatcca
    18061 cgcatccatt aatgtcagtg gtgacaagtt ttacataaaa cacaccgctg ttaaaataag
    18121 tgtgggcagc aattggtgaa gaagaattat gaggaaaggt tccatcgccc caataccata
    18181 aaaaaccttc attgcaactg ccataagcat cagtagcgtt cgtggcatct acatttatag
    18241 tatagctttt acaggcaaca gaatctctgg taaattctgc cgatatattt ctgacgcgaa
    18301 caatattgga atcaatatca ggtgcacaac cagatgttgt gttgaatgaa gttaatttta
    18361 cccaatagtc gcctgtagca gcataggtat gtgttggatt tacgtttcct gaattaatta
    18421 atgctgtgcc atcaccaaaa tcccagttcc aggtggtagc atcgttaatc gttgcaggaa
    18481 aactatattt aaatggagta ttacaatttc cggccacata aaatttcgcc ataggtcctt
    18541 taacagtgac agtttgtgta gctgtgctga tgcaaccatt gtttgatgta gaaagtgtaa
    18601 ttgtctgttg ccctgtttgt gtgttgaaac tccagatagg attggtttga ttgtaacaat
    18661 gcgacagcat tcctccatca ccagaaaaat gataaatatt attggtgtca ggattcagaa
    18721 cattaaactg tacaggtgtg tatgcacata tagttgtagg aacaacagta aatgatgttg
    18781 taacagcaga acctacataa atcggaatta taaaagaagt gtctttgcat cctgcagaat
    18841 ttgtaactac taacctcaca tagtaaatac ccggtgtgtt gtaggtgtgt gtaaccacac
    18901 cattgcttcc tgtagtttgt gtgccatcgc caaataaata ggtgtaattt acaatagtct
    18961 cacgagaacg actcgaatca gaaaaagtaa cagtcattgg agcacagcca attgatttat
    19021 caggcataaa acgagcagtg atactaaaca cagtgtcgtt tttttgtttt gcaacagtgg
    19081 cagaacaacc atgtacactc gtaaaagtca gctgatcggt gtagtagtaa taatcataaa
    19141 tggtgtattg gtttgtatca ggacgataaa aggtatgact tgggtttgca agatttgatg
    19201 tggtgccatc accaaaaatc cattgatagg tacctgcacc agaagttata ttggtatatt
    19261 gaacagtaaa tggccaactg cagctgtaac ttggtgttga ggtaaagttg gcaacaacat
    19321 tttctacatg aatagtaatg gttgtatcgt cagtacaaat accatttaat acctgaagtg
    19381 ttacctgata ggttcccggt gcataggtat gaatactgtc aacgcctgaa gttccatcac
    19441 cgtagttata gaatggattg gtaccaattg attgatttct gaaagttact gtaggacaaa
    19501 ccgtatcatt aactgcacca acggcaaaga atgatgctag tggaggatta atgacaacct
    19561 gagtagtaac tgtcttagga caattgttgt tatcagtaac cgtaagtgaa atgttataag
    19621 ttccactcgt gggatagttt attgaaggag gtgttacaac cgttgatgta tttccaccac
    19681 caaaattcca aaagtaggta agccctgttc ccgttgtaga atttgatgta gatgctgagg
    19741 cagaaaaggc aactgtaaat ggcggtgtgc aacctattgg cgggctgggc actgttgtaa
    19801 ttacagcgtt aggttgaatg cttgtattaa tataatttgg aacagaaata gaagtgtcgc
    19861 agccattggc atcagtcact tttagcgaga ctgtaaaact tccggcaaga gaataaatgt
    19921 ttgttggtga agaattgttt cctacattgg ttgcgccatc accaaatgcc cactgcctcg
    19981 aaacaattgc agtaccacca ccgccaacag atgaatcatt aaaatgaaca gttaatggaa
    20041 cacaacctga aagcggaggt gttgccgtaa gcgatgtaaa tggttttcgg aagacattta
    20101 tttttattga atcaataaaa gcatttcctg agagcaagcc tgtgcagaca acagtaaaac
    20161 ttcctgctgt aaaaaaagta tgtaccggat taggcaggtt tgatgaggtc atatcaccaa
    20221 aatcccaatc aatatttgtc agccctgcag gcgaggtaaa attaacattt accaaaggca
    20281 cacagccact tgtggtgctc gctgtaattt gtgcactgac atttgatata atgcctataa
    20341 aaagaatgaa aaagaataat cttttcaaga tgcgtgaaga aaaaagtgaa atgttaattt
    20401 ttgttttcaa atttacggct tttcggtaac gatgtaaaaa atttatatgt ttgtagtgtc
    20461 tgtatgcggt aagatgaatt ttcaatcact caaaagtata gcctttgcat gaaagcagca
    20521 actttaattt atgtcatttt attttcctgc ctttttgcag tatcggatag caatgcacaa
    20581 ggcttgtgcg ggactgtgat tgatacagct aaaatgcaag ccgaagcagc agctaattta
    20641 aattcttcac aaaatcaacg cgggcttttt ttccctgtaa actgtttgaa taaaacactt
    20701 tctatcactg cctatatttt tcgagatagt ttagctgccg atggcatcac acaggcatca
    20761 attttgtcgg cattgcaaat tctgaataat gactttgcac ctatgtgttt gtcgttcagc
    20821 atctgtgagt ttgtgtatgt caacaattac aattacaatg atttttatgc attgaagcat
    20881 gaaaatgaag tttataattt atactatact ccaaatacta taaatgttta tttcagcaac
    20941 acattatacg acaatacaaa ctcatcggtt tgtggatatg cccattttcc gggtggacgc
    21001 gatatgattt ttctgaataa atcttgtgta ggcgatggca aaactttctc gcatgagatg
    21061 ggacattttt ttggacttta tcatacgttc gaaactgcca atggagttga attaattaat
    21121 ggcacaaatt gtctggtagc cggtgatctg atatgcgata cacctgcaga ccctaatgga
    21181 ttaaacggtg cagactgtca gatgcttcct tatttgcctg acccttccgg caattggtat
    21241 gttccgcaaa tcggcaacat catgtcatac tattctcccg gttgcaaatg tggttttact
    21301 actcagcaat ttaactggat gatacaacaa tatctcacca acagaaacta cctttggtaa
    21361 aagaattttc ttattagttc aattcaaatc attatgaaat cacgaaaaac aaatcagttt
    21421 attctgatag ctgccattac agcattgtta tcagcaaatg tttttgcaca agacaaagcc
    21481 aaatcaacaa ctacaccggt taaagttact tccgtagaag gaattacgga atataaactc
    21541 agcaatggct tacgtgtatt actgtttccc gatccatcaa aatctaccat cacggtaaac
    21601 attacttact tggtaggttc gcgcatggaa ggttatggag aatcaggaat ggcgcattta
    21661 ttagaacaca tggtgtttaa aggttctacc aaccatacaa atattccgca ggaacttact
    21721 gcacatggtg caagaccaaa tggaactacc tggtatgaca ggacaaatta ttttgaaaca
    21781 tttaatgcaa cagaagaaaa tcttcgttgg gcattaagct tggaaagtga cagaatggtt
    21841 aattcattca tcaataaaaa agatcttgaa tctgaatttt ctgttgtgcg aaatgaattt
    21901 gaaagaggag aaaacagtcc ttcaagtgta ttggaagagc gtgtaatgtc aactgcttat
    21961 ctatggcata actatggaaa gtcaaccatc ggatcaaaag aagatattga gcgtgttcct
    22021 attgaaaacc tgaaagcatt ttatcataaa tactatcagc cggataatgc agtacttact
    22081 attgccggaa aaattgatga gcaaaaaaca ttacaattag taagcgaata ttttagtcct
    22141 attcctaaac ctgaacgtaa attgcaggag acatttacag tagaacctgc acaggatggc
    22201 gaacgtaatg ttgttttacg cagagttggt gatgtgcagg ttgcctcttg cggatatcat
    22261 atccctgcag gtgctcatgc cgactatgca gcaattgaag ttttgataga agcattaaca
    22321 aacgaacctt cgggaagatt atataaagca ttggtggaaa caaaaaaagc tacaagtaca
    22381 tcgggctatt catttcagct taaagatccg ggatatgcct atttctatgc agaagtatta
    22441 aaagacaaac ctcttgacac agtcatgaat accatgaaag gtatttttga taacctgagt
    22501 aaaaatccta ttacaaacga agaggcagag cgtggcaaga atgccattct caaacaactt
    22561 acactgatgt ttaacaacag cgatagggta gggctttcac taagtgaatt tattgcacaa
    22621 ggcgactggc gtttgtattt tatttatcgt gatgcattag agaaagttac tactgccgat
    22681 gtaaacagag tggctgcaga gtattttaaa ccaagcaacc gtactacagg actttttatt
    22741 cctgatgcaa caccggacag agctgtgatt cctgcatcac ccgatgttaa tgcattggtt
    22801 aaagattata aaggcaaaaa agcgttggca gaagcagaag catttgatcc ttcgccaaca
    22861 aacatcagca acagaataaa acaggcaact atttccggtg gtgcaaaata taccttactg
    22921 aaaaaaacca ctcgtggtgg caatgtcagt gtaaacatta cattgagaat gggaaatgct
    22981 gcagtgatgc aaaataaagg tactgctgca tcattcactg cttcgatgtt gcgcagagga
    23041 acaaagaata aatcatttca gcaaattaat gatgagattg acaaacttaa gtctaatgta
    23101 ggcattaacg gtaacggaca aactgttaat gtatcggtaa caactactaa agaaaacctc
    23161 agcggtattc taaatctgat aggtgaaatc attaaacaac ctgcatttgc acaggcagaa
    23221 tttgataagt taaaagagga acaaatttct gatcttgaaa gctcacgcag cgaaccacag
    23281 gcaattgcat tcagggtata tagtaaaaca ctgaatcctt atccaaaaga cgatttccgt
    23341 tatgaaatga gttttgacga agaaaaagaa gcattgaata agcttacatt ggctgatgta
    23401 aagaattttt atgagaggtt ttataccact tcaaatgcta cagcagcaat tgttggtgat
    23461 tttgatgagc catcattaat tatttctctg aacaatttac ttgataaatg gggttcaccg
    23521 gttttatatg agcgtgctaa agaaccatat tttgatgtta ctcctgtaaa cgagaaaatc
    23581 aatacccccg acaaagcgaa tgctttgatg gtggcaggaa tgaacttacc attgaaagat
    23641 gacaatgcag attatccggc actgatggtt ggaaattata tgttgggcgg aggtttcttg
    23701 aattcacgcc ttgcaacacg catcaggcag aaagaaggca tcagctatgg tgtaggctca
    23761 caattgcagg caagttcttt agatgagtca ggtggatttt tatcttatgc aatttacaat
    23821 cccgataact cagataaact gattgcagca tggaaagaag aggttcagaa aatgttgaac
    23881 gaaggattta ccaatgcaga actaacagat gctgtgaaag gattaataca ggcacgttca
    23941 gtaagccgct ctcaggacag agagttgtgc agcaaactta acagctatac ctatctcggt
    24001 cgctccataa aatgggatga ggaatttgaa aataaaatca aagccttgaa agttgaagat
    24061 gtaaatgcag caatgaaaaa atggattaat ccgaataaag tttcttttgt gcaggcagga
    24121 gattttgaga ggaagaagta agcgttattt gataaagtat ctataaaaat atttacaaag
    24181 aatcctgctt aatgcaggat tctttgtttg tataaaccag aacacttaga agtagtagtt
    24241 tgaaaatatt tgaacagaaa cttttgcttt cagagctaaa aaatttaaaa taaaagttcc
    24301 tttttttaaa aaaatctgtt acttttaccc aattaaagaa ccggccaact aaacaagata
    24361 tatctggtca tgaaaaagat atttggtttt attgttgttt ttctaacgtt gtctataaac
    24421 aatttgcttt atgggcagca gccaagtagt tgtaccaatc ttgattttga actcggaaac
    24481 ttcgtgggct ggactggaaa aacaggttca tgctgcccta tcagtatgtt ttcaaacggt
    24541 attgttaccg gacagcatac tattatgtca ggtacaggat ttgatcctca ctctaacaat
    24601 cagattcctg ttgttgcgcc cggtggaact tactctgcac gtttaggtaa tgatcaggca
    24661 ggctcacaag cagagcaact ttcttattcc tttcaggtta ctccacaaac agaattattt
    24721 atttatcgct acgcagttgt tttagaagac cctgtgcacg accctacgga acaaccacgt
    24781 tttgagattc gtgtatttga tcaggcaggt aatcctgttg gttgtggaac ttacaatgtg
    24841 gtttcaggtg ccggcattcc cggttttcaa acctggctcg atcccaatat aggcagcgat
    24901 attcatttta agacatggac aacagtaggt attgaacttt catcctacat gggtcaaact
    24961 gttaccattg aattttctac aggtgattgc tctttaggcg gacactttgg ttatgcctat
    25021 gttgatgcct attgcagcag catgactgtt acaagcgatt actgccccgg tagctcaaca
    25081 gcactactga tagcacctcc cggatttgca tcctacctgt ggagtaatgg tgcaacaaca
    25141 caaacaatca ctatcaacaa ccccgtcatt ggtgctacct tttctgttca gatgacatct
    25201 gtgacaggat gtgtggtaac attgaatcag gttattgccg ttacacaact gtttgctaac
    25261 tttaacttta atccgggctg cgaaagtgga actcagtttt tcgattcatc ttatgttctt
    25321 tcaggttcac cggtgaatca atggttgtgg gattttggtg acggcaccac ttcgacaatg
    25381 cagaatccgt ggcatcaatt tccggcatca ggaaattata atgtaacact tacggcaacc
    25441 aatgcaggag gttgtacaaa atcaattact aaatcaataa gtattttgcc atcgcctgtt
    25501 tcagacttta attttacacc tgcatgtcca ggtcaagcta tatcttttaa tcagaatgca
    25561 agttttccat caggaacaat tacaagttat gaatggttgt ttggcgatgg gcttccttca
    25621 tcagcagtac ctgacccaac acatgtttac agtggtatgg gaccttatga tgtttcactt
    25681 attgttacag gaaataatgg ctgtgtagat actattgtaa aacctgtggc accaatgcct
    25741 gatccagtag caggatttac attcagtgtg cagtgtatga ataataccgt tacgtttact
    25801 gatgtttctc aactgaccgg tggcaccata actgcatggc aatgggattt tggagatggc
    25861 tcaccattag ataatactgc aaatcctgta catgtttaca acggtggcgg caattacgca
    25921 gttcaactga ttattacagg cagcaatggt tgcactgata ccattaccaa aggtgtggct
    25981 atggcaagtt tgccaacagc agcttttact aacagtggcg catgtgaatc gcagtttaca
    26041 gcatttaatg atgcatcaag tcttacaggt ggcagtattt catcctggct gtggtacttt
    26101 ggtgatggaa caaattcaac tttgcaaaat ccatttcacc aatataacgg tacaggaagt
    26161 tttaatatat cattggttgt tttaggcgat aatggttgcc gcgattctat ggcacgtagt
    26221 attacagtta aaccttctcc tgtagcatca tttacctcat tgcccgtttg tcccggacag
    26281 caggctcagt ttaacagtac tgctgttgca cctgccggtg aaaccataag ttcgtggatt
    26341 tggaattttg gtgatggcag tgcgcaaaca gcgggtcaga atgttaatca cactttcacc
    26401 ggtgccggca cttacgcagt gatgagtatt gttggtgctt caaatggttg cattgatagt
    26461 gttgtaaata atatttcaac ttcaataaca cctgtagcta aattttctgc accatcagga
    26521 tgtgccttag tgccgctatc attttccagt acatcaacta tatcttcagg taccataaca
    26581 ggatgggatt gggattttgg cgatggaact gcacacggca ctacagcagc aacaacacat
    26641 gcctatgcag caccgggaag ttacaataca tatttaactg ttaccagcaa caatggttgt
    26701 acacatacag tattgcatga agttagtgtt agcggcaagc ctgtgccatc atttacaaac
    26761 tcttcaagtt gtgttaatga cagcatcaat ctgaacagtg tttcaacatc tgcaaatgga
    26821 agtatcaatt acttgaaatg ggattttggc gatggtactt tcctcacagg aacagatagt
    26881 gtttttcatg tttatgcaga tacaggtttt gtgcagattg gattaattgt aggtaataca
    26941 cttggttgca gcgacactgt ttacagacag atttatatta agcccttgcc tgttgccgga
    27001 ttccgatggg atgaaatgtg tcagtcagca ccgggaagtt tttatgatac ttcagtattg
    27061 ataaatggaa atatttcagg atggctttgg gattttggaa gtggcactac caatactgcc
    27121 aacccgcagt ttgcattttc aaattacgga gttcaaccgg ttacattaac agtaacgggt
    27181 agcaataact gtaaagcaac aatctctcga aatattaccg tgcgaccaaa gccattagca
    27241 caatttgttt catcaagtgc ctgtgtcaat gcagctgaaa cttttcagaa tacatcatta
    27301 atatcctcag gaaatattgt aagctggcaa tggaattttg gcgatggaca cagttctata
    27361 tctcagcatg gacaaaacac ttttacaact gacggatatt atcaggttca attatttgca
    27421 atatcagact ttgcatgtgc tgacacagca cttgacacta ttcgcgtgtt tcctttacct
    27481 gttgccaact ttttcgcacc gcatgtatgt ttgggtaatg ccacagtttt taatgacttg
    27541 tcacaaccaa tttccgatag tttaattaca tggtcgtgga attttggtga tggctctact
    27601 tcgacaatgc caacaccgtc acatattttt gcacaggata caatttaccc ggtttcactg
    27661 acagtacaga atgaaggctt ttgcagtaag acaattacaa agaatgtaat catcaggcct
    27721 ttgcctgaag ttggctttac atttgccaat ccatgtgttg gcagccctgt acagtttcag
    27781 gatacatcgg ctttatcagg atataatatc aattggtggc aatggaattt tggtgacagt
    27841 gctactgcgt caatagcatc accggtacat acatattcac tgtctggaaa tatttttgtg
    27901 aaactgattg ttcgggctac aaatggttgt tcagattctg tttctgttcc tgttttagtt
    27961 aatcatttac ctgatgcagt cttttctgta agtgatattt gtgagcaagc tgtattaacg
    28021 ccaaacaatc aatcagcatt atcgggagga aacattacaa acactttatg gcaatggggt
    28081 gataatacaa gtgattcaat tttttcacca acacactttt atcaaagtga tggcaacttt
    28141 aatataaacc ttgaggttac aggaagtaat ggttgtaaga atgatacgac cattgccata
    28201 gtaattcatg ataaacctat agcctcattt acacatagca atgcatgtca gttagctcct
    28261 gttgcattct atgatcagtc ttctactgta aatgactcat tgcaaatttg gcagtggcag
    28321 tttggtgatg gtgatcaatc aaatctacaa agtcctgtgc atgattttgg tgcttatggt
    28381 gtttattata cagagctcat tgtaactaat agtttcggct gtcgtgacac agttgccgat
    28441 tccgttactg ttgcacctaa accgcaggca gcattttcat cagtaaatgt ttgtaaagat
    28501 caaccaatga tttttaataa ttcaagtagt gttgttgcag ggagtattgt ttccaatcag
    28561 tggagttttg gtgatggctc ttctgctgct gtagctaatc ctacctatac atatttaagt
    28621 accggtgatt atgaaattac tttagtagtt acaacagcca atcaatgtgt tgatagtata
    28681 aaacatattt atcgtgttta tcagttgccc acagcgggat ttattgcaga tactgttgaa
    28741 ggatgtcagc cccttttggt tttattttct gatagctcag ttgccaatga agggttaatt
    28801 accatgtgga attggaactt tgataatggt gcatcagata ctttaacaaa tcctgcagca
    28861 attgtttatg atcaggcagg atatttcagt cctgtattgt atattgaaac aaatttagga
    28921 tgtcgcgata cctttttacg acaggattac attcatgttt ttgcaaaacc tgaagctgca
    28981 tttattgcag agccattaag caatacaatt ttacatccac tgattgagat taaagatcag
    29041 tcatggaata cagtatcatg gttctatgat tttggtgata ccacaaatac aacagagcaa
    29101 aatccttcac attggtataa gcaacctgaa cactatacca ttattcaata tgttgaaagt
    29161 gcagatggtt gcaaggacac agcattaatc agcatagaaa ttaaaaatga ctttactttt
    29221 tacatgccta actctttcac tccaaaccaa gatggcttta atgaaacatg gaatgctttt
    29281 ggtattggca ttacagaatt taatgtgcag gtttttaatc gctggggcca gttactattt
    29341 acatcaaatg atttgagtaa aggatgggat ggaacctatt caggaaacaa agtacaacaa
    29401 ggtgtttacg tatataaagc aaccgttcgc gatttattca ataagcaaca tttttatgtt
    29461 ggaaatgtaa acgtaatcag atagtaaccc cagtaaatga atttccggat taaagagaat
    29521 gcactgatag catggattgc agcccgtttt ttaggctata aaacaatggc aatagtcatt
    29581 ggaaaaacaa tttacctgca tggtgcatca gttaaaactt ttcagagtga ttccaaatgg
    29641 atgaagcatg aactcaagca cattgaacaa tttaaacgct acggtttttt acggtttatt
    29701 tttctctata taattgaatc aattcgtcat ggatatatca ataaccgctt tgaaattgaa
    29761 tgtagagaag ccgagaatca ggattgaatt ttattctgaa atagaaaaaa attattcttg
    29821 caaagtgcca aatcgtccct gattaaaatc ttcaaaagcc tgcactaatt cctgtcttgt
    29881 attcattaca aaagggccat gtgctgctat tggttcatta atcggttcgc cactaagcac
    29941 cagataaact gcattttcgt ttgaatcaat tgtaaaactt tcaccatcat tatccataag
    30001 agcaaaatga ttggtatcta ctttttcggt tccatttata gtaatgcttc cttcaataac
    30061 caataacgct gtgttgaaat ttttaggcag attcatttct gttttagcat cttttgccat
    30121 cttaacattg taaagatgca atggtgtaaa agtagaggca cttcctttaa ctcctttata
    30181 ttctcctgca ataatttcaa tttctgtttt tttatcatca gatttaaaac gattgatttt
    30241 tgaattttct aatgcctgat attttggctt actcatcttg tcttttgcag gaaggttcac
    30301 ccacaactgt accatctgaa attcgccacc tgttttgctc cattctttct catgaaattc
    30361 tttatgcaaa acaccactgg ctgcagtcat ccattgaaca tctccttcac caattactcc
    30421 gccaccaccg ctgctgtcat gatgttctac cttaccttta taggcaatgg taactgtttc
    30481 aaatccacga tgcggatgta ctccaacacc cttaggagta tcactggggt caaagtgaaa
    30541 tttagaatta taatctaaca tgatgaatgg attcattctt tgcatgtcaa gacgataacc
    30601 acttggtatg aaattatgta ctctgaagcc atcacctaca aaatgtggtt cacgtggact
    30661 aacaactatt tctacagatc ttttttccat gataatgtat atttatagtg caaaattatt
    30721 atcagccctt cctttttaca ataacgtaga ttaagtattt gttttaagtc agtttactta
    30781 caaagagaca gaaaaaattc ataaagcaga ttaaaccatt atttcataaa atagtctatt
    30841 agtttgaaaa agcaacaata ttaattttaa aaatatgaaa caaagaattt ctgtagtaat
    30901 gggcttatta attatggtaa ctatggtaag tttatctttt aagcctgaca attggcgcat
    30961 cttaggcaca aaaacagtgg actatcagtt agacaaagat gtgttagatg tgcagttgcg
    31021 tgacggtgtt tttcagaaac tgaaatttgt tgtcagagga ggtgctttga atatgcataa
    31081 agttattgtt cacttcgaga atggaggcga acaggaaatt gatatgcgtc atagttttga
    31141 taagaacagt caaagcagag ttattgacct gaaaggaaac aaacgcatca ttgaaaaaat
    31201 tgttttttgg tacgatacta aaaatgcttc aaaccgaaaa gcaaaagtga ctgtgttagg
    31261 acgttgatga aataatattt taagaccgta aaatcttagt tagtgggtga ccttttgcaa
    31321 gctcgtccac taacttatct aagtacctga cttttttcat aaaaggattt tcgatttcct
    31381 cgattttata accgcaaatg gtgcctgtaa ttttacttgc atttggatta attttggcct
    31441 gc
//
LOCUS       DAOOOI010000015        29916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_270_length_29916_cov_11.243394, whole genome shotgun sequence.
ACCESSION   DAOOOI010000015 DAOOOI010000000
VERSION     DAOOOI010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 29916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29916
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_270_length_29916_cov_11.243394"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2556
                     /locus_tag="PKX59_03785"
     CDS             <1..2556
                     /locus_tag="PKX59_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ90019.1"
                     /translation="VAYSGFKQEVVVNAETIDVGSVKLVYSPGTTTTEADIPVVTLDD
                     DELQSNSAQSSVSSVLGASRDAFTSAANFNFSVARFKTRGYDTENFPTLMNGIAEEDL
                     TNGRTAFSSWSGLNDVLRSRESTSGLNPSTYAFGGVGGSYSIDSRAGRQRKQIQATYS
                     LSNRTYDNRFMLTYGSGFNKKGWAYSASISKRWAKEGFIKGTYYDGFAYYLGVSKVVN
                     KHEFDFTVLGSQYESARSSSNTKEVYDIAGDHYYNSNWGYQNGEIRNANIGRSHQPVF
                     TLNHEYKISNNTTWFSALSYTTGKRESTALNWFAADPRPTYYRNLPSWQTDSAAAAQV
                     YNTLKANPDLLQINWDNMLLANDQNFQTVNNADGVAGSTVSGKQALYLIENRVIQTNR
                     LSFNTTFNTILNDNITTSTGLSYQKQTSRFYKEAEDLLGAEFFVDIDKFADQDFQDEQ
                     LSQSDLNHPNRIVREGDRFGYDYEAHITHAQAWTQAVAKYSNIDIFGAIQAAYTSVYR
                     EGNYKNGLFPNDSYGKSKTYVFPDVGVKAGATYKVNGRNYVFANAGYMTRAPFFNNMY
                     LSPRSRNTTVGDLTSEKITSVEGGYLHIAPKIKGKLKLYYTNFKDGIESRSYYLQGFG
                     TNSYVNYTLSGIEKVHAGTEISVDANLGMGLSAMAVASIGQYYYSSRPTATITVDNTN
                     TVQSTGEVVYMKNLHVDNTPQNAFTVGLNYRSKKFWYVGVNANCFSNSYVEASPARRT
                     GDIIDGVDENSQAWEKILGQERYDAQMTLDLSAGWSIKLNNHIKALKRNTFFLINLNI
                     NNLLDNKDIITNGYEQGRLDKSAVLSGRPDVFASKYSYAFGRTYMISFILRMN"
     gene            2583..4004
                     /locus_tag="PKX59_03790"
     CDS             2583..4004
                     /locus_tag="PKX59_03790"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOZ90020.1"
                     /translation="MIKNIKITTAFAILAITLSTVSCVKKDDFDNAPTTNVDPDIVTN
                     ATVSSVVTLANATGPVQITSDLTFSAIIVADDKSGNYYKEMIVQDTTAGISILIDQPS
                     YFTSLFIGRRVFVKAKGLYIQAVNGTPKIGVLNAGAVAEIPAAEIGNYIVGGKWGLTV
                     VPAKRKLNALTDADLNTLVQFDSVAFAAAEVGLPFGDAANKSTVNRNLKDCSGNSLIV
                     RTSGYANFATTLIPCKAGSVTAIYQKYNTTPQVFLRDMNDVPANIERCDGVVCTPVYV
                     SIDSIRTLFSSGATTVPGGFHIKATVISDYTTSALDTRNVIVQDATGGICLRYSTAPN
                     FSMNSQYDFNVGGLTLGEFNGWLQISNMAGGLSITGLAPITPTVIDLATATANFEQYE
                     SMLIKVNSVTINNGTATTYGANGGSLTISDGSTPNTVVLYCRSAASFASSATPTTPVS
                     ITGILTQFNTTKQFQIRNLGDIQ"
     gene            4072..6291
                     /locus_tag="PKX59_03795"
     CDS             4072..6291
                     /locus_tag="PKX59_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOZ90021.1"
                     /translation="MKRLISIVFFLLLLQNSFAQLVSIATARTSPAGTQVTVTGVCVN
                     GQELGNIRYINDGTGSVAVFSTQLNTTVRGDAIMVTGVLSDYFGLLEITNLTSFQNLG
                     GGNNLPTPIVTTPLLLDESTESHLVRIDGVVFNNGGGTFMGNANYAFTSNGESGQVRI
                     AANTNLVGQPIPSGAVSMIAVCSQYQTVYQLLPRDQNDIFISSSFYMTSPPVQQNIST
                     AGFDVNWTTNLPASSFIEYGLTPALELGILNGTSGSANHTVTLSGASPSQIYYVKAFS
                     VNGNDTATATVKIYMTASLSSGDMKVYFNRSVNNNYAWTPANNAIQLPGTFQDTIAAY
                     INRSQQSVDIAIYNFENSGTSQIVQAINDADNRGVAVRIIYDGGNANSGLALLNPGIN
                     MLPSPTTPPGYYSIMHNKFVIIDAYANDVNKPIVISGSTNFTNAQLNNDANNLLIIQD
                     KSLAIGYTMEFEEMWGSSTLQPNPANSKFGPDKKDNTPHEYNIGGNRVESYFSPSDNV
                     NNQIMTTVETADQQMQFALLVFTRFDVAYAAEDRIVNHGVDAYGIVDDTGSGGGQAYS
                     ILNAVMGSKLMLYNHATQTGLLHHKYLIVDQNNPSSDPLVLTGSHNWSTTANQKNDEN
                     TLIIHNRNIANQYYQEFVKRFTDNGGVLGLSETEEGMTTTLYPNPASDHLFISLNHAL
                     ENNYQFGLFDVAGKQVMQMNLQQGNSGIISLGRLNKGIYFYQIRGEKQINSGKLIIN"
     gene            6461..7159
                     /locus_tag="PKX59_03800"
     CDS             6461..7159
                     /locus_tag="PKX59_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOZ90022.1"
                     /translation="MSILLQITQAVTDSATALQPAVKAEQSISFLDLVIKGGPIMIPL
                     GLLSVAAVYIFFERYFTIRKSSNIDKNFINQIKDFVHTGNVDAAKALCRNTNHPVARM
                     IEKGLLRLGKPIRDIESSIENVGKLEIYKLEKNLSFLGTIAGIAPMFGFLGTIFGVIK
                     IFYQISLENSLQIDTISGGLYVKMITSAAGLLIGVAAYAGYHYLISLVDRAVNKMEIN
                     AVEFIDLLEEPVKK"
     gene            7220..7594
                     /locus_tag="PKX59_03805"
     CDS             7220..7594
                     /locus_tag="PKX59_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOZ90023.1"
                     /translation="MTGEVFVGSLNDIMFFLLLFFLITSTMATPNVLKLLLPNAKTSK
                     PAVKHPVTISVTADMQYAVNNQVVAYDQLESSILTQLQGQEDPTAILKVDKSVQVQGL
                     VDLLDIGARNKIKMVLATNTVK"
     gene            7683..8459
                     /locus_tag="PKX59_03810"
     CDS             7683..8459
                     /locus_tag="PKX59_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90024.1"
                     /translation="MHTKIPPFGGGGGAESSNLGFVELSTGDIQPESENVSENPHPVT
                     ATPPVAVQEQNYNTQETEESAVVEAKKEKTPEKKVTRPVETPVKTEVKKAEPVVPKVN
                     AEALYPGKKNKSVSQGTSNTGSGDQGTRDGDPNALYQGKGTGQGGSGSGGGEGDGSGP
                     GKGSGSGPGFSYDLAGRSVRAKPTIYDNSQETGKVVVSIKVDKYGNVIDAVPGARGST
                     TTSSNLFRKAKDAAFKAKFNQSPQGVEEQRGTITFVFLVQ"
     gene            8461..9747
                     /locus_tag="PKX59_03815"
     CDS             8461..9747
                     /locus_tag="PKX59_03815"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOZ90025.1"
                     /translation="MNYTETLNYLFSKLPMFHRIGAAAYKDNLDNSIALDNILGHPHK
                     SFKSIHIAGTNGKGSTSNMLAAVLQQQGYKTGLFTSPHLKDFRERIRVNGKMIPKKYV
                     SDFVQQYMNEFDVVQPSFFEWTVALAFDFFRHSKVDVAVIETGLGGRLDSTNIIQPQL
                     SVITNIGWDHVNLLGDTLEKIAVEKAGIIKENIPVLIGETTIQTKNVFLKKAIENSAP
                     IYFASNQCKVELKKQHLEYLIVDTQSKNGEVYKNLKLSLAGEYQLKNVATVLSAIELL
                     KDSCFVIEKKNVKKALTDIQQLTGFRGRWQVLGTKPLIIADTGHNVDGIKTVIRQICK
                     ISFKRLHIVIGMVNDKDIMPVLKLLPKHATYYFCNAKIPRAMPAKELKNKAIEVGLNG
                     QIYTSVKQALLSAKKAAVAQDLIFVGGSTFTVAEVL"
     gene            9832..15243
                     /locus_tag="PKX59_03820"
     CDS             9832..15243
                     /locus_tag="PKX59_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOZ90026.1"
                     /translation="MKFRFTILFMAVVFLANADQTIFPVNSVWKYLDNGTNQGTTWRD
                     SSFNDAAWSSGTAKFGYGEGDENTVVSYGPNPSNKYITTYFRKAISISNLATFSAFEL
                     GVRRDDGVVVYVNGVEAWRDNINGVVSDTSLAASDASDDGNSIITATIPTSYFVNGNN
                     VIAVEIHQFVVNSSDLSFDMRLTGLETPPPSNVTIFPLNSVWKYLDNGTDQGTTWRDV
                     SFSDATWTTGPGEFGYGDGDEATVVSYGPNPSNKYITTYFRKSVSIADVNAYTSFQIG
                     VKRDDGVVLYVNGVEVWRDNIAGTVSYNTLATADAADDGSSVLTANIPTSYFVNGTNV
                     IAAEVHQFVVNSSDLSFDMSLTGMGNTPPTMPSIVIGSYLNVATPTSIIVRYRTDITT
                     MSKVVYGTTSGVFTDSTVSASPVTEHIVQLSGLLPNTKYYYAVKSGNNFIQNNPAENF
                     FITPPVAGTAKPTRIWVIGDMGFGSVEQNQVRDAYYNYTGSTYTDLWLWLGDNAYNSG
                     TDAEYQNNVFNNRYEKMLRQTVVWPAAGNHEMYASNAVTETGPYYELFNFPTNGEAGG
                     VPSGKKAYYSYNYANIHFVVLESTTAAFRTIGGAMMNWLQTDLAANTQKWTVVYFHHP
                     PYSMGSHNSDTETELIEMRQNFVPILEQYKVDLVLCGHSHNYERSFMIKNHFGSENTF
                     NSSNLVDGGSGAGATPYIKTASSNFQGTVYAVAGTGGILEGIQASWPHSAMYSYSSST
                     YGSMLLDIDGDTLYAKFLDNTLPNPTIYDQFTIIKQCDQQVTLVPFSNVCSGQSAFAL
                     TGGSPAGGSYSGTGVSNGMFNPAVAGIGTHTITYTYTNGFCSDSATSTITVTTGGQIA
                     TINPSGNITLCQNDSVLLSANSGTGLTYQWQLNGSDISGATSQTYTANGAGNYTVAIT
                     SSCGTVVSSATIVSAASSVSISPSGSVNLCSGPVTLSVNTGVGFNYQWKNNGVDINGA
                     TNSSLEILSAGNYSLLATDSTGCASLSNIVNATGSAHVISALGSTTICAGGNVTLSAQ
                     TGSGFTYQWYRNNGVISGAINSTYKATSHGNYYCRTTAPGCTGNSNIIVVAVINNPTP
                     TISANGSLNICSGSSVILSTNTFPGVTYQWERNGSAVNGATSQLHTTTLAGTYRVSQT
                     ANGCSKKSPSISVKIVAGPTATINANGSVNLCNGQTVTLNANTVTGAAYQWLADGVNI
                     AGETNQSLVVNTSGNYQCRITTTCAALSNVITVTASSMQISISPSTTQNICQGNSVQF
                     STTNDPGNSYQWNIDGSAIPGAISDSYSANVSGIYSVSITNGCGTQTSQAVTVNVLPV
                     SAVISPDAAQVICSGGSVNFTANSGFGNTYQWNLNGTPINGATNQTFNANAAGNYSVT
                     VSNSCGVYTSTSVSVSVGSISATISPDIAQTICSGDSVLLSANPASGISYQWYLDGNA
                     IGGANTATYFAKSQGNYTVNIVSSCGSSTSPSVALAVISLSSSITPAATQIICPGSFV
                     LLSANTGNGLTYQWKKDGVLISGATQSTFLADETGIFTVDVTNSCGTLESNQVTVNAA
                     LINSSITPSGTSTVCSGTPQTFSVLPTAGATYQWYRNNVAINNATNVSLNASSSGQYY
                     VKVHLSGCNIVTSNTAILSVQTNTTPVVTVTPGTTLCNGQQATLSSNIYSGVAIQWQH
                     EKVNIPGATSQTYQASVGGQYRVVHTFNGCVRNSAEKSIKFVSCRSEGYESDLEMHVS
                     AEVFPNPFKEVTNIKLSGFDISSTTITVTNILGEMINRFNPDSENIEWKRNGNAPGIY
                     LIQITDGYNHRITQKIVIE"
     gene            15349..18504
                     /locus_tag="PKX59_03825"
     CDS             15349..18504
                     /locus_tag="PKX59_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOZ90027.1"
                     /translation="MTTKQFLLAFLFCLPLFSKADTTLVSFNDAWKYFDNGYLPATNW
                     NQIMYNDSAWAIGNGEFGYGDGDETTVVSYGGDPNNKFVTTYFRKTISLNNPYDFASY
                     HLDLKRDDGAIVYINGTEVFRSNMTSGSVGFNTPAYSDCVDDGMALQSIVLVPADFLA
                     GVNVIAVEVHQINNVSSDLSFDLQLNAYNNFVPQLLRQPYLQMVADSAITIRWRTNVS
                     CQTAVLYGNDPDSLNFSVSDSANVIDHEIRLTGLSPDTRYFYALGDGTNVLQGDSNNY
                     FKTAPVKGMPKPVRIWSMGDTGINTIDQNNVRDAFLNYNDSLPIDLLYMLGDNAYYSG
                     TDQEYQDAFFQNHYEDILKNIPVFSVAGNHENYTANAITQTGPYYDIFTFPKNGECGG
                     VPSGSEAYYSFDYSNIHFIALETNTLSLLQPGSDMLNWLQSDIAATQQKWKIVVFHNS
                     PYTKGGHNSDTETDLILIRQNIMPILDQHKVDLVLCGHNHVYERSYFMDGHYGLSSTF
                     DSTMVVDSSFGELPQPYLKKSSKNYKGLVVVQAGCSGVLDPIGPQWPHPAMKRYYDQE
                     LGSLLIEIETDTLTVTFVDNNVSSPQVLDEFSVVKECNVVPQLTVPVAKVCVTDDPVQ
                     LSATPIGGFFSGLGVVDTVFNPTVAGQGIHTIAYTYNDIYGCHVTDTKTIEVVGGVPS
                     QPFGIVGSILVCPPSNNEVLSIQSLPDADSYIWSASAGVNLTSSNTDTLIKFDINTVL
                     PQYNISVSALNICGSSIPSLVNITSVKSFPVSLTGNSVICPGDTSVYTVNVFNSLSSY
                     WWQPSEHVFLNGQSDIINTFNTLHVNASVDSSFTEGKICVASKEACLRNNAVSFIDLS
                     TKPPVPGVISGPAAVLGGSNGVVYSIVPVSGATGYFWTVTGDAILTAGQFGNSVTVDF
                     LPSFTGGYVGVKSQSNCDESIEVSKRIKTYIIQVSQNTVSQTRIANEYNQDKIVSVYP
                     NPIGNEMHVKLLLQDASSNVLVVLKDVAGKEVGKWSKKVFSDEDDITLNVSGLSSGVY
                     FLCVTTDNMQWNGVVVKQNDH"
     gene            complement(18498..19088)
                     /locus_tag="PKX59_03830"
     CDS             complement(18498..19088)
                     /locus_tag="PKX59_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOZ90028.1"
                     /translation="MDNKKHKELLGEIENTTEKLQANAENILNWIKHQNKRIKKQISS
                     VAIGALAEDITEQFMHLTYNKGTRLISTISHDDIIQSDSNLLSIILHNIISNATKFTE
                     NGFIKIYSKQNNQYEITIEDNGKGMSEQQLTRITKLINKQPLNEEDEQLDNKEGFGLG
                     YIIISELVQILNGKIEVQSKLGLGTKVTISLPLTYQ"
     gene            complement(19099..21543)
                     /locus_tag="PKX59_03835"
     CDS             complement(19099..21543)
                     /locus_tag="PKX59_03835"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HOZ90029.1"
                     /translation="MHKLKVLFLVFLFTFFKLSAQNSYSIQNYTIKKGLPDNNVLGLF
                     YEKNGYVWVVYGSGLARFDGINFKKHFTSDAPYLGLYFYQTTNADKIMIEPSGNIFQI
                     KGLTSDTLRKGKINSLNYINIKGTLPDIKTYLELSTPHVNITVDKSWILTPLIMFPLP
                     DNSYIVRTKKGIAHFKGKNKLKELNLDHLQNRKFISANGNIYVFANDSEIYRVDLTSW
                     SLVKCKLKGDLSSCKNYSRKITNTFWDFNNSDPCVQVDYTLYSFFPDSLNANLISTVQ
                     LTNQLPNNCLISSIIYNEDQNLIMIGTDTRGLFVYKKRNFKSLEFANPEPGTNNTYFC
                     QIALNDSTVYTDWNREFTINGGKQSKLNLARNYSENICIDKKGNYWYAENNSLVRFNP
                     KTKAKKYIYYTGDELPLSFFEEGDTMWVGLIKSFGCVVNDTLKHIKILDNDESNSNIF
                     QLLRFEGKLWICNYTGIYKYDTQSGKLDTLPELALKYTYHFKAINNYLLIGTYGRGYY
                     MYKNGKTVKMPNGRFEALRQVHNFVPDSLGFLWMATNNGVIKTKFAELVHYFNDTTYQ
                     VSYFHYSEEDGISNPEFNGGCDPNYLRLNNGYVSLPNVEGLVWFYPEKIEDPRSDYPI
                     SIDQFIADDSIYYTSNNIKVPNSVQYVRIEFSNPYWGEPENLFCEYKLEGYNKQWIYL
                     SPNESALEFSNLKSGEYKFTIRKQTGLSKDYTEATIAFHIENKYYETIWFWLAMVGLA
                     LLIIIIVARIYAFNIRRKNILLEKNVVLRTSELQTANNELSESIKIKDKLISIISHDL
                     ITPLRFITMVAKKGGE"
     gene            complement(21549..22196)
                     /locus_tag="PKX59_03840"
     CDS             complement(21549..22196)
                     /locus_tag="PKX59_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ90030.1"
                     /translation="MNAFSDISLLIADDHVIIRRGLKFLLETHFGKFNIVETDSSKGI
                     LDSLEKNNFTHLILDMQLSDGNLLEIFQEIKQQFPLLPILIYSMSPEEIFAKRLNNLG
                     ASGYLNKQSSEEEVVKAMELFFTGRKYVSQKYFDQQHGVQKGKSENPFSNLSERETEV
                     LLLLLKGKSVKEIASHFDLKSNTVATFKARIFDKIGVNNLMDLQNSARAYNIPIV"
     gene            22647..>24854
                     /locus_tag="PKX59_03845"
     CDS             22647..>24854
                     /locus_tag="PKX59_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90031.1"
                     /translation="MLNFNLQAQTTVTIGAGSQSGTQSNSAAGDCGPMYRSTASSAFV
                     YSRHHYLYTQSEISSAGIPSGNLITKLAWYNQNGAGTNSPCIFQIWMKNSALTDVGVG
                     GQNWSTLTSGSTLVYNSSTHSVPATVGWHDIVLSTPFTYTGGAIEISVNFDISGGSSP
                     YTTNGFSWNKDPITGRTLSYVGSAAPGATLPNLRTVRAQIQMTYQSGAPCSAPPTAGS
                     STATPSSGLCVSSNFVLNLSGNSIGSGQTYQWQESTTFGGPYTDLGSSSSSPLLNTTA
                     TTTKYYQCKVTCSGNDQYSTPVLVTVNPSFPGGTYTINSALPTSGSNFQTFNDFKSAL
                     NCGIAGAIVVNVVSGSGPYNEQIEFGEIANASSINTITINGNGNVLTFGATVSTAPGT
                     LVLNGTDYMTVNNLIVEGTGATYALVCHLWNNANNNAFNNCTFNAPANGTITTMVPFS
                     ISGSATSATTSGNSGSNNVITGCTMFSGYYNTVLVGNSGLPSTGNQVINCNINDQNLY
                     ATYCLYQDGVVLRGNTLSRPTRTSLGTYYGIYISTGCTNALVEKNRIRNTWATNTGST
                     GTQYSIYCTIDATLGNENKFFNNVISDIEFNGTIYGMYMSGSDYWQAYHNTISLDQTT
                     STATSTTYGVYATGTTAYDFRDNIVTISRGGTGTKYGVYYSTPAAATSDYNNIYMNAG
                     AGTNYVAYSGAPYTTLSAYQIASGKDANSSSVDPFYLAPGSPTYNYTPNNGLSN"
     assembly_gap    24855..24883
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24884..>29916
                     /locus_tag="PKX59_03850"
     CDS             <24884..>29916
                     /locus_tag="PKX59_03850"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90032.1"
                     /translation="SNDTGTPVGVTDDINGAPRSLTTPDIGAYEFSLGGLDAGITWFT
                     PTSPTSPGMKSITVNIANTQAQTINSVQLSYSDGGAPVTQTFTALNLLSGNNTNLTFT
                     TQYNLTSSVTMTVDILAVNGGSDAVSGNNSDTYYLCVAPSGTYTINSALPTGGSNYQS
                     FTDAIQALSCGITGPVVYNVVAASGPYNEQITIPQIDGASTTNTITFNGNGNTLTFNG
                     NAGNPNTLALNGADYFTFNNLNIEATNTTYAIACHLYSGADSNKFNNCTFTTSITGTT
                     TQLAAFVISGSPTTATGLGVSGNSNIVDSCHMVGGYYNTVIYGTSSAPYQENNEIRNS
                     TISDFYGYGIYNVYCRNTIISGNTVERLNRTNTSTVYAIYCSTSSYNVLVEKNRVRRL
                     FDAQQTSTSSAYCMYNGGSGAAGFENKWINNLVYDIKSKGSLYGMYCPSYNYVHVYHN
                     TISFDDASATGTTSTYGIYAYGTSTEVRNNIISITRGGTGTKYCLYFSTNTAVMSNNN
                     DLYINAPSGSNSIGYASGAQATLANWQAANGGAWDQNSQSVNPNYANIAIDNYKPTNV
                     ALDNIGAALGVTDDITGAARSLTTPDPGAYEFAAANKDVGVSVFVGPNPDGCFTNAET
                     IVVEIKNFGVQTIDFSVDPVTVNCVITGPVNTTVSAILSTGTLASGATMNVSLSPTVD
                     GTANGTYTLTPSTSMASDEDNTNDALTPINITVGPIAGTVSSSLSDICVSDDPVLTVT
                     GHYGGDIQWQQSTVGAGGPWTNVGTNSATYSPGTITQSTWYQALTVCNSNTATSNVVA
                     VTVNNPQLNSLIPGTRCGYGSVALGINVNMGTTGNWYVDSTSNTPIATGTTFNTPNLN
                     STTTYYVAPSSGGSNGITIPGDGDWDHVTTTGSFQTTLITGAYMILTVTQPFTLTSFD
                     IYPSAAMNTAFGIEARTGSASGPMFMSYSGNTTVVNSGAPTVAQTVNVNWVLPIGTYY
                     IGFVGTNPNSWRSGLATHSYPWVLPGYASLDYYLTPNYQYYFYNLKISTGCEGVRVPI
                     TATVTSPPALSVITADPTLCPGNTTTVNVSSINDPNYTYSWTSNPAGFTASGAGPHNV
                     SPSVTTQYIVTATDNTVGPNAGCVAVDSVEVITGSVLTAGTVASSDTMFCQSGIPTLS
                     VTGADGGAIQWQSSTVSASGPWTNVGTNAVVYAPGTLTQTTWYQVMVHCQLTEVYSNV
                     VEVTVNNPQLSSSNGDTRCGPGVVNLTASALSGTVNWYTQSSGGTPIATGNTYSPTVS
                     NTTTYYVAATSGGGTESVGRPTYSNTPNTNGNAWGLVFNVVTTPIDLQSVTVYSVGAG
                     GTISVQLTDNVGTVLSTAGPFSYPAGSVATPVAVVLPVNFTVPVGTGYRLLSAAFSGG
                     NLIRETSGITYPYTSPSSNVVITSGYISGTTFSTYYWFYDWQISTGCEGVRVPIVATV
                     NPSPAVNIAVQYPTLCTGQSATLTASSSNPDYTYSWTPGGSGASIVVAPGTTTTYTLN
                     AIDNTAGPYSQCTAIATQLVSVVNANPPIPIASSGGDVCLGSDIFLSSDNIEPGQFSG
                     NTYEWTGPNSFISNIQNPSISSPGVSYSGTYNVIVTNQYGCTEAASTSLSVNPNPTLT
                     IDSVQNVSCLGGSDGIIYVSASGGLAPYSYTSDFINFNTNGIMNNLPTGASIIYAADG
                     NACQAQITG"
BASE COUNT         9332 a         5232 c         5847 g         9476 t
ORIGIN      
        1 gttgcctaca gcggctttaa acaagaagta gttgtgaacg cagaaacaat tgatgtgggc
       61 tctgtgaaat tggtttacag cccgggaaca actacaacag aagccgacat tcctgttgta
      121 actcttgatg atgatgaatt gcagtctaac tctgcacagt caagtgtgtc tagtgtttta
      181 ggtgcatctc gtgatgcttt tacatcagcg gccaatttta atttcagtgt tgcacgattc
      241 aagacacgtg gttatgacac agaaaatttc ccgacattaa tgaatggaat tgctgaagaa
      301 gaccttacaa atggtcgtac tgcattttca agctggtcag gtttgaatga tgtgctcagg
      361 tcaagagagt caacttcagg actaaatcca tcaacttatg cttttggtgg tgttggcggg
      421 tcatacagta ttgactcacg tgccggaaga caacgtaaac aaatacaggc tacctattca
      481 ttaagcaaca gaacgtatga caatcgtttt atgttgactt atggtagtgg atttaataag
      541 aaaggatggg catattcagc ttctatttca aaaagatggg ctaaagaagg ttttataaaa
      601 ggaacatatt atgatggctt tgcatattat ttaggagtat caaaagttgt caataagcat
      661 gagtttgatt ttactgtctt aggtagtcag tatgaaagtg cccgttcatc atcaaacact
      721 aaagaagttt atgatattgc aggcgatcat tattataatt caaactgggg atatcagaat
      781 ggcgaaatcc gtaatgccaa tattggaaga agtcatcagc ccgtgtttac attaaatcat
      841 gagtataaaa taagtaataa cacaacttgg ttttctgctt tatcatatac aacaggcaag
      901 cgcgaatcaa ctgcactaaa ctggtttgct gctgacccac gtccaactta ttacagaaat
      961 ttaccttcat ggcaaacaga ttctgctgca gcagcacaag tttataatac attgaaggca
     1021 aatcccgatt tattacaaat taactgggat aatatgttgc ttgctaacga tcagaatttt
     1081 cagacagtaa acaatgccga tggtgttgcg ggttcaacag ttagcggtaa acaagcatta
     1141 tatttaatag aaaatcgtgt aattcaaaca aaccgattga gctttaatac tacatttaat
     1201 actattttaa atgataatat tacaaccagt acaggcttat cttatcagaa gcaaacttca
     1261 cgtttttata aagaagcaga agatttgtta ggtgcagaat tttttgttga cattgataag
     1321 tttgccgatc aggattttca ggatgaacaa ttaagtcaga gcgacttgaa tcatccaaac
     1381 agaatagttc gtgaaggtga tcgttttggc tatgactacg aggcacacat tacccatgca
     1441 caggcatgga cacaagctgt tgccaagtat agtaatatag acatcttcgg tgctattcag
     1501 gcagcttata cctctgttta cagagaaggt aattataaaa atggattgtt tccaaacgat
     1561 tcttatggca aatcaaaaac ttatgtgttt cctgatgttg gcgtgaaagc cggtgctacc
     1621 tataaagtta atgggcgcaa ctatgttttt gctaatgcag gttatatgac aagagcgcca
     1681 ttctttaata atatgtattt gtcaccacgt tccagaaaca cgacagtcgg agatcttaca
     1741 agtgaaaaaa ttacatctgt agaaggaggt tatttacata tcgcacctaa gattaaaggt
     1801 aaacttaagt tgtactatac aaacttcaaa gacggtattg aaagccgctc atattatctg
     1861 caaggttttg gcacaaacag ttatgtgaat tatacacttt caggaattga aaaagtacat
     1921 gcaggaacag aaatttctgt tgatgctaat ctgggaatgg gactttctgc aatggctgta
     1981 gcttcaattg gtcagtatta ttatagctca cgtccaacag caactattac tgttgataat
     2041 acaaacacag ttcaatcaac aggcgaagta gtttacatga aaaacctgca tgtggacaac
     2101 acacctcaaa atgcatttac ggttggattg aattaccgtt cgaaaaaatt ctggtatgtt
     2161 ggtgttaatg ccaattgctt ctctaattct tatgttgaag ctagtcctgc aagacgcaca
     2221 ggtgatatca ttgacggagt agatgaaaat agtcaggcat gggaaaaaat tcttggacag
     2281 gagcgctacg atgcacaaat gactcttgac ctgagtgcag gttggtcaat taaactgaac
     2341 aaccatatca aagctttaaa gagaaatact ttcttcctga ttaaccttaa catcaataac
     2401 ctgctcgata ataaagacat tatcaccaat ggatatgagc aaggcagatt ggataaatca
     2461 gctgtgcttt caggacgacc tgatgtattt gcttcaaaat atagttatgc ttttggcaga
     2521 acatatatga tcagctttat tttaagaatg aattaataac taattagaaa tataataaaa
     2581 caatgataaa gaatattaaa atcacaacag catttgcaat tcttgcaatt actttaagta
     2641 ctgtttcctg cgttaaaaag gatgattttg acaatgcacc taccactaat gttgatccgg
     2701 atattgttac caatgcaaca gtatcgagtg tggtaacttt agctaatgca acagggccgg
     2761 ttcagataac ttctgattta actttctctg ccattattgt ggctgatgac aagtccggta
     2821 attattataa agagatgatt gtgcaggata caactgcagg tatcagcata ttaattgatc
     2881 agccatctta ctttacttct ttgtttatag gaagaagagt atttgtaaaa gcaaaaggcc
     2941 tatatattca ggctgtaaat ggtactccta aaattggtgt gttaaatgcc ggtgcagttg
     3001 cagaaattcc tgctgcagaa atcggaaatt atattgttgg tggtaaatgg ggattaactg
     3061 ttgttcctgc aaaaagaaag ttgaatgcat taacagatgc cgacttaaat acactcgttc
     3121 agtttgatag tgttgcattt gctgcagcag aagttggtct gccatttggc gatgctgcaa
     3181 ataagtcaac tgtaaatcgc aatttgaaag attgttcagg caattcacta atcgttagaa
     3241 ctagcggcta tgcaaatttt gccacaacac ttattccctg caaggcagga agcgtaactg
     3301 ctatttacca gaaatataac actacaccac aggtatttct tcgtgatatg aatgatgtgc
     3361 ctgcaaatat tgagcgttgc gatggtgtgg tttgtactcc tgtttacgtt tcaatagaca
     3421 gtataagaac tttattttca agtggcgcaa caacagttcc cggtggtttt catatcaagg
     3481 caactgtaat ttctgattac actacttctg cacttgatac aagaaatgtt atcgtacagg
     3541 atgcaaccgg tggaatttgc ttgcgttata gtactgcgcc aaatttttca atgaattctc
     3601 aatatgattt taatgtgggt ggtttgactc ttggagaatt taatggatgg ctgcaaatta
     3661 gtaatatggc aggaggtctt tcaattactg gattagcacc aatcacacca actgttattg
     3721 atttagcaac tgcaactgct aacttcgaac aatatgaatc aatgttgatt aaagtaaata
     3781 gtgtaactat taataatgga acagcaacta cttatggtgc taatggtgga agccttacta
     3841 tttctgatgg cagcacacca aatacagtag ttctctattg tcgtagcgct gcatcttttg
     3901 caagttcagc tacaccaaca actcctgtta gcattacagg tattttgaca cagtttaaca
     3961 ccacaaaaca atttcagatt agaaatttag gtgatattca ataaacatta aatatttttg
     4021 acgcagtgca gcatgttcct gcactgcgtt tttattttaa taggtactcc aatgaaacgt
     4081 ttaattagta ttgttttttt tctactgctg ttgcaaaata gttttgcgca gttagtgtct
     4141 attgccacag ccagaacatc acctgcagga acacaagtaa cagttacagg tgtttgtgtt
     4201 aacgggcagg agttaggaaa tatccgttat atcaatgacg gcacaggcag tgttgctgtg
     4261 ttcagtacac agttgaatac aacggttcgt ggcgatgcca ttatggttac aggtgtgctt
     4321 tcagattatt ttggtcttct tgagattaca aatctgactt cgtttcagaa tctgggaggt
     4381 ggtaataacc tgccaacacc aattgttaca acacctttgc ttcttgatga gagcacagag
     4441 agtcatttag tacgtattga cggagttgtg ttcaacaacg gtggtggaac ttttatgggt
     4501 aatgctaact atgcttttac ctcaaatggc gaaagcggac aggtgcgcat tgcagcaaat
     4561 acaaatcttg taggccagcc aattccttcc ggtgctgtaa gtatgattgc cgtttgcagt
     4621 cagtatcaaa cagtgtatca acttttgcct cgcgatcaga atgatatttt tatatcatct
     4681 tcattttaca tgacctcgcc acctgttcag caaaatataa gcacagcagg ttttgatgta
     4741 aactggacta ccaatttacc tgcatcttct tttattgaat atggacttac acctgcgctt
     4801 gaactgggaa ttctcaacgg cacatcaggt agtgcaaatc acacagtaac actttcagga
     4861 gcatcgcctt cacagatata ttatgtaaaa gcattttctg taaatggaaa cgatacggca
     4921 actgctactg taaaaattta catgactgct tcactttcaa gtggcgatat gaaagtatat
     4981 ttcaataggt cggttaacaa taattatgca tggacacctg caaacaatgc aatacaactg
     5041 cccggaactt ttcaggatac cattgctgcc tatatcaacc gctcgcaaca aagtgtagat
     5101 attgcaattt ataattttga aaacagtggt acatcacaaa ttgttcaggc aattaatgat
     5161 gcagacaaca gaggtgttgc agtgagaatt atttatgatg gaggtaatgc aaacagcgga
     5221 ttggcactat tgaatccggg aataaacatg ttaccttctc caactacacc accgggttat
     5281 tattccatca tgcataataa gtttgtgatt attgatgctt atgcaaatga tgtaaataaa
     5341 ccaatagtaa tttccggatc aaccaatttt acaaatgcac aactcaataa cgatgcaaac
     5401 aatttgttga ttattcaaga taaatcgctt gccattggtt acacaatgga gtttgaagaa
     5461 atgtggggca gcagtacatt gcagccaaat cctgccaatt caaaatttgg tcctgataaa
     5521 aaagataaca ctccacatga atacaatatt ggaggtaatc gtgtcgaatc ttatttcagc
     5581 ccttctgata atgtaaacaa tcaaataatg acaacggttg aaacagcaga tcagcaaatg
     5641 caatttgctt tattggtgtt tacacgtttt gacgtggcct atgctgcaga agaccgtatt
     5701 gtgaatcatg gtgtagatgc ttatggaatt gttgatgata caggtagtgg aggtggtcag
     5761 gcctatagta ttttaaatgc tgttatgggt tcaaaactga tgctttacaa tcatgcaaca
     5821 caaacaggat tgttgcacca taaatacctg attgttgatc agaacaatcc ttcaagtgac
     5881 ccattagtgc tgacaggctc gcataactgg agtacaactg ccaatcagaa aaatgatgaa
     5941 aatactttaa tcatacacaa cagaaacatt gccaatcagt actatcagga atttgttaaa
     6001 cgatttacag ataatggcgg tgtgcttggt ttgagtgaaa cagaagaggg tatgacaacg
     6061 accttatatc ccaatcctgc atccgatcat ttgtttatca gccttaatca tgcacttgaa
     6121 aataattatc aattcgggct ttttgatgtt gccggtaagc aggttatgca aatgaatctg
     6181 caacagggca attccggaat tatttcttta gggagattga ataaaggcat ctatttctat
     6241 caaatcagag gtgaaaaaca gataaattca ggtaaactga ttatcaatta aacgaacaat
     6301 aatttttcag gaagttcaat tttaggttaa ataatttata cttttttcct tttccttccg
     6361 ttagccttat caacaaaact gtgtttaaaa tagcagtgac acttgattat ttaataaggg
     6421 catgggcgta cttttactgt tctaaaattt cgaattcaac atgagcattt tattgcaaat
     6481 tactcaagct gttactgata gcgccactgc attacagcct gcggtgaagg ccgagcaatc
     6541 aatttcgttt cttgatttag ttataaaggg cggaccaata atgattccat taggattgtt
     6601 gtctgttgct gctgtttata tttttttcga gagatatttt actatcagaa aatcatccaa
     6661 cattgacaaa aattttatca atcagataaa agactttgtc catacaggta atgttgatgc
     6721 agcaaaggca ttgtgccgaa acacaaacca tcctgtggcg cgtatgattg aaaaggggtt
     6781 gttgcgtctg ggtaaaccta tccgtgatat agaatcatca atagagaatg ttggtaagct
     6841 cgaaatttat aaacttgaaa agaacctgag tttcttgggg acgatagcag gtattgcacc
     6901 catgtttggt ttccttggaa caatcttcgg agtaataaaa attttctatc agatatcgtt
     6961 ggagaatagt ttgcagatag acaccatctc aggtggtttg tatgtgaaaa tgataaccag
     7021 tgcagccggt ttattaattg gtgttgcagc ttatgcaggt tatcattatc tgatttcgtt
     7081 agttgatcgc gcagtaaaca aaatggagat taacgctgtt gagtttattg acctactcga
     7141 agagcctgta aaaaaataat tgtgaaattc agtttataaa agcatcatta tggcattgaa
     7201 tctgagaagg cgaaataaaa tgacagggga agtttttgtt ggctccctca atgatattat
     7261 gtttttcctt ttattatttt ttctgattac atcaacaatg gcaacaccaa acgttttgaa
     7321 actgttgttg ccgaatgcaa aaaccagcaa gcctgctgtg aagcatccgg tgacaatttc
     7381 tgttactgcc gatatgcagt atgcagtaaa caatcaggtg gtggcttatg atcagttaga
     7441 aagttctatt cttacacaat tgcaaggtca ggaagatcca actgcaattt tgaaagttga
     7501 taaaagcgtt caggtgcaag gcttggttga cttgcttgac attggtgccc gcaataaaat
     7561 aaaaatggtg cttgctacca acacagtgaa ataacatgga agctccggct ataaaaagaa
     7621 actctttaat tttaacattg gtgctacata gcatcttatt gtttctttta tggtggttta
     7681 caatgcacac aaaaattcca ccattcggtg gaggaggtgg tgcagaatct tctaaccttg
     7741 gttttgtaga attgtcaaca ggagatattc agcccgaatc agaaaatgtt tctgaaaatc
     7801 cgcatcctgt aacagccaca ccccctgtag ctgttcagga acaaaattat aacacacagg
     7861 agacagaaga gagtgcagtt gttgaagcca aaaaagaaaa gactcctgag aaaaaagtta
     7921 cgagacctgt tgaaactcca gtaaagactg aagttaagaa agcagaacct gttgttccta
     7981 aagtaaatgc agaagcatta tatccgggca agaaaaataa atctgtgtcg cagggaacat
     8041 caaacacagg cagtggtgat cagggaactc gtgatggcga tcctaatgca ttgtatcagg
     8101 gcaagggaac cggacaaggc ggcagtggta gcggaggagg tgaaggtgat ggttcaggtc
     8161 ccggaaaagg tagtggttca ggtcccggat tttcttatga tttagcagga aggtctgtgc
     8221 gtgcaaagcc aacaatttat gacaacagtc aggagaccgg taaggttgta gtttctataa
     8281 aggttgataa atacggtaac gtcattgacg cagtgcccgg tgccagaggt tcaactacca
     8341 caagctccaa tcttttcaga aaagcaaaag acgctgcttt caaggcaaag tttaatcaaa
     8401 gtccacaagg tgttgaagaa caaagaggaa ccattacctt tgtttttctt gttcagtaaa
     8461 atgaactata ccgaaacact gaattatctg ttttcaaagt tgccaatgtt tcatcgcatt
     8521 ggcgcagcag cttataaaga taatttagat aacagtattg ccttagataa tatactcggt
     8581 catccgcata aatcttttaa atctattcac attgccggaa ccaatggcaa aggctcaact
     8641 tccaatatgc tggctgctgt attgcaacaa caaggttaca agacagggtt gttcacctcg
     8701 cctcacctca aagatttcag agagcggata agagtgaatg gtaagatgat tcctaaaaaa
     8761 tatgtaagtg attttgtgca gcaatatatg aatgagtttg atgtagttca gccatcgttt
     8821 tttgaatgga ctgttgcatt ggcattcgat tttttccgtc attctaaagt tgatgttgca
     8881 gtaatagaaa caggtttggg tggacgattg gattcaacaa atattattca accacaactt
     8941 tctgtaatta caaacatcgg ttgggatcat gtgaatcttt taggtgatac acttgaaaaa
     9001 attgcagttg aaaaagcagg aataataaag gagaatattc ctgtcctgat tggtgaaaca
     9061 acaattcaga ccaaaaatgt ttttctgaaa aaagcaattg aaaattcagc accgatttat
     9121 tttgcaagca atcaatgtaa agttgaactt aaaaaacaac atcttgaata tttaattgtt
     9181 gatactcaaa gtaagaatgg tgaagtatat aaaaatttga aattaagcct tgccggtgaa
     9241 tatcaattaa aaaatgtggc aacggttttg tcagcgatag aattattgaa agattcatgt
     9301 tttgtaattg agaaaaaaaa tgtaaaaaaa gcacttacag atattcagca attgacagga
     9361 ttcagaggaa gatggcaggt gttgggaact aaacctctca ttatcgcaga caccggccac
     9421 aacgttgacg gcattaaaac agtaattcgc caaatttgta agataagttt taaaaggctt
     9481 catatagtaa ttggcatggt taacgataaa gacataatgc ctgtcttgaa gctattgcca
     9541 aaacatgcta cctactattt ttgtaatgct aaaatccctc gtgcaatgcc tgctaaggag
     9601 ttgaaaaaca aggcgataga agtggggctc aatggtcaaa tttatacatc tgtcaaacaa
     9661 gcattgttaa gtgcaaaaaa agcagcagta gcacaagatt taatttttgt tggtggaagc
     9721 acatttacag tggcagaagt gctctgattg tgtgtaacta ttcacccaaa aaactgcact
     9781 ttttcagtgt agttagggta attttgtgac gcttaaaaaa tcaagattac gatgaagttt
     9841 agatttacaa ttttgttcat ggcagtggtg tttttagcca atgccgatca aactattttt
     9901 ccggttaatt cagtatggaa atatttagac aatggaacga atcagggaac aacctggcgc
     9961 gactcatcat tcaatgatgc agcatggagt agtggtactg caaaatttgg ttatggcgaa
    10021 ggcgatgaaa atactgtagt gtcttacgga ccaaatcctt caaacaaata cattacaacc
    10081 tatttcagaa aagctattag tatttcaaac cttgcaacat tctctgcatt tgagttaggt
    10141 gttagacgtg atgatggtgt tgtagtgtat gtaaatggtg ttgaagcatg gcgtgataac
    10201 atcaatggtg ttgtgtctga tacttcactt gctgctagtg atgcctctga tgatggtaat
    10261 tctataatta ctgccaccat tccaacatct tacttcgtaa atggcaacaa cgttattgca
    10321 gttgaaatac accagtttgt tgttaacagc tcagacttaa gctttgatat gcgacttacg
    10381 ggccttgaaa ctccgccacc atcgaatgtg acaatcttcc ctttaaactc agtgtggaaa
    10441 tatttagata atggaaccga tcagggtacc acttggcgtg atgtttcatt tagcgatgct
    10501 acatggacta ctggcccggg agaatttggc tatggtgatg gtgatgaagc tacagttgtt
    10561 tcatacggac ctaacccttc aaacaagtat atcacaacat atttcagaaa atcggtttct
    10621 attgctgatg ttaatgcata tacttcattt cagattggag taaagcgcga tgatggtgtt
    10681 gttctctacg ttaatggtgt agaagtatgg cgtgataata ttgcaggaac tgtatcctac
    10741 aatacattgg caacagcaga tgctgctgat gatggaagtt ctgtattgac tgctaatatt
    10801 cctacatctt acttcgtaaa tggcaccaac gttattgctg cagaagttca tcagtttgtt
    10861 gtgaacagtt ctgatttaag tttcgatatg tcattaacag gaatgggaaa tacaccgcca
    10921 acaatgccgt caatagtaat aggttcttat ttgaatgtgg ctacacctac aagtattatt
    10981 gtgaggtata gaacagatat tacaacaatg tcaaaagttg tttatggtac aacttcagga
    11041 gtttttacgg atagtacagt tagtgcatcg cctgtcacag aacatattgt tcaacttagc
    11101 ggcttactgc caaacacaaa atattattat gccgttaaat caggaaataa ttttattcag
    11161 aataatccgg cagaaaattt ctttatcaca ccacctgttg ccggaacagc taaacccaca
    11221 agaatatggg tgataggtga tatgggtttt ggaagtgtag aacagaatca ggtgcgtgat
    11281 gcctattata actataccgg aagcacatat acagatcttt ggttgtggct tggtgacaat
    11341 gcttacaatt caggaactga tgcagagtac cagaataatg tgtttaacaa cagatatgaa
    11401 aagatgcttc gtcagactgt agtttggcca gcagcaggta atcatgaaat gtatgcatca
    11461 aatgcagtca cagaaacggg gccttattat gaattgttta acttccctac taacggagaa
    11521 gccggtggtg taccatcagg taagaaagca tattattcct acaactatgc aaatattcac
    11581 tttgtagtac ttgagtcaac aacggcagca ttcagaacta ttggtggtgc tatgatgaat
    11641 tggcttcaga cagaccttgc tgccaataca caaaaatgga cagtagttta ttttcatcac
    11701 cctccatatt caatgggtag ccacaactct gatacagaaa ctgaattgat agaaatgcgt
    11761 cagaactttg ttcctattct cgagcaatat aaagtagatt tagtgctatg tggtcacagt
    11821 cacaattacg agcgttcgtt tatgattaaa aaccattttg gatcggagaa tacattcaac
    11881 agttcaaatt tagttgatgg cggaagtgga gcaggcgcta caccatatat caaaacagca
    11941 tcgtctaatt ttcaaggaac tgtctatgct gttgcaggaa caggaggaat tcttgaaggt
    12001 attcaggcaa gttggccaca tagtgccatg tattcatatt cttcttcaac gtatggttca
    12061 atgttgttgg atattgatgg agatactttg tatgctaaat ttttagataa tactttacct
    12121 aatccaacaa tctatgatca gtttacaatt atcaaacaat gtgatcagca agtgacactg
    12181 gtaccattca gtaatgtatg ttccgggcaa agtgcttttg cactaacagg cggaagtcct
    12241 gcaggaggca gttacagtgg tacaggtgta agcaatggca tgtttaatcc tgcagttgcc
    12301 ggtataggca cacatactat tacttataca tatactaacg gattttgtag cgattctgcc
    12361 acatcaacaa ttactgttac aacaggagga cagattgcaa caattaaccc ttcagggaat
    12421 attacattat gtcaaaacga ctctgttcta ttatctgcaa acagtggaac aggcttaact
    12481 tatcaatggc aattgaatgg ttctgatatt tcaggtgcaa cttctcaaac ttatacagcc
    12541 aacggtgcag gcaactacac tgtagctatc acttcatcat gtggaacagt tgtttcatca
    12601 gctacaattg ttagtgcagc ttcttcagtt agtatttcgc cttcaggatc agtaaacctt
    12661 tgctcaggtc cggttacact ttctgtaaac acaggtgtag gctttaatta tcaatggaaa
    12721 aataatggtg ttgatattaa cggagctaca aatagtagct tagagatatt aagtgcagga
    12781 aattattctt tattagccac tgactcaacc ggatgtgcgt cattatctaa tatcgtaaat
    12841 gctactggta gtgcacatgt aatctctgcg cttggctcaa caactatttg tgccggagga
    12901 aatgttactc tttctgctca aacgggatca ggtttcacct atcaatggta tagaaataat
    12961 ggtgttattt cgggtgcgat taattcaact tataaggcaa caagccacgg caattattat
    13021 tgtcgtacta cagcacccgg ttgtacaggc aattctaaca ttattgtagt tgcagttatt
    13081 aataacccta caccaactat ttctgccaat gggtcattga atatttgtag tggaagttca
    13141 gttatacttt caaccaatac ttttcccgga gtaacctatc agtgggaaag aaacggttct
    13201 gctgtaaatg gtgcaacatc acaattgcat acaacaacac ttgccggaac ttaccgtgtc
    13261 tcacaaacag caaatggttg ttctaaaaaa tcgccatcaa tttctgttaa gattgttgca
    13321 ggaccgacag ctactattaa tgctaacgga agtgttaatt tatgtaatgg acaaacagtt
    13381 acattaaatg ctaacactgt taccggtgcg gcataccaat ggcttgcaga tggagtgaat
    13441 attgccggtg aaactaatca aagtttggtt gtaaatacat caggaaatta tcaatgcaga
    13501 attaccacaa catgtgctgc actctctaat gttattactg ttacagcaag ttcaatgcag
    13561 atttctattt caccctcaac tactcaaaat atttgtcagg gtaatagtgt gcagttttca
    13621 actaccaatg acccgggtaa cagctatcaa tggaatattg atggtagtgc aatacccgga
    13681 gccatttcag attcttattc tgctaacgta tctggcattt actcagtttc cattactaat
    13741 ggttgcggaa cgcaaacatc gcaagctgtt actgttaatg tattacctgt ttcagcagtg
    13801 atatcacctg atgcagcgca ggtaatttgt tcaggcggaa gtgtaaactt tactgcaaat
    13861 tcaggttttg gaaatactta tcaatggaat ttaaatggaa ctccaataaa tggagcaaca
    13921 aaccaaactt ttaatgccaa tgctgcagga aattattctg ttactgtttc aaatagttgt
    13981 ggtgtttata catctacttc ggtatcggtt tctgttgggt caatctctgc aacaattagc
    14041 cctgatattg cacagactat ttgctcaggc gatagtgttc tgttatcagc aaatcctgca
    14101 agtggcatat cttatcagtg gtatttagat ggtaatgcaa ttggtggagc aaatacagca
    14161 acttattttg caaaatcaca gggaaattat acggtaaata ttgtgagttc atgcggaagt
    14221 tctacttcac cttctgttgc acttgctgta atttcacttt caagtagtat aacacctgca
    14281 gctactcaaa ttatttgtcc gggttctttt gtgctgttgt cagccaacac gggtaatgga
    14341 ctaacttatc aatggaaaaa agatggtgta ttaattagcg gagctacaca atcaacattc
    14401 ttagctgacg agaccggaat ttttactgtt gatgtaacaa atagctgtgg cacgcttgag
    14461 tcaaatcagg taactgtaaa tgctgcacta atcaattctt ctattactcc atcaggaact
    14521 tctactgttt gttcaggtac tccgcaaaca ttttctgtgt taccaacagc aggagcaact
    14581 tatcaatggt atagaaataa tgttgcaatt aacaatgcta caaatgtttc tttaaatgca
    14641 tcaagctcag gccaatatta tgtaaaagtg catttgtcag gttgcaatat tgtaacatca
    14701 aatacggcaa tactttctgt tcaaacaaac accacacctg tagtaaccgt aacaccggga
    14761 acaacattat gtaatgggca acaggcaaca ttaagcagta atatttattc cggtgttgca
    14821 attcaatggc aacatgaaaa agtgaatatt ccaggagcta catcacaaac ttatcaggca
    14881 agtgttggag ggcaatacag agtagttcat acgtttaatg gttgtgttag aaattctgct
    14941 gagaaatcaa ttaaattcgt tagctgccgt tccgaaggtt atgaatctga tttggaaatg
    15001 catgtttcag cagaggtatt cccgaatcca tttaaggaag taacaaatat taagttaagc
    15061 ggatttgata tctcctctac aaccattaca gtgaccaata ttcttggtga aatgattaat
    15121 aggtttaatc ctgattctga aaatattgaa tggaaacgaa atggaaatgc gccaggtatt
    15181 tatctgatac aaattacaga tggatataat catcgcatca cacaaaaaat tgtaattgaa
    15241 taattggtga attgtgtaaa actttaactg aaaaccgact ttttataagt cggttttttg
    15301 tttcttagta ggagttgaaa gttaagtgtt tattacaatt tatttgtcat gactacaaaa
    15361 caatttttac ttgcattttt attttgtttg ccactttttt ccaaggcaga tactaccttg
    15421 gttagtttta atgatgcatg gaaatatttt gataatggat atttgcctgc tacaaactgg
    15481 aatcagatta tgtataatga ttcagcgtgg gcaattggaa atggagaatt tggatatggc
    15541 gatggtgatg aaactacagt tgtaagttac ggtggtgatc caaataataa atttgtaaca
    15601 acttatttca gaaagacaat cagtttaaac aatccgtatg attttgcctc atatcatctt
    15661 gatttaaaaa gagacgatgg tgcaatagtt tatataaatg gcacagaagt tttcagatct
    15721 aatatgacct caggaagtgt aggtttcaat acacctgctt attctgattg tgttgatgat
    15781 ggaatggcat tacaatcaat agtattagtt cccgcagatt ttcttgccgg tgtaaatgtt
    15841 attgctgttg aagtgcatca gataaataat gtcagttcag atttatcgtt cgatcttcaa
    15901 ttaaatgcat acaataattt tgtgcctcaa ctgctgcgtc aaccttatct tcagatggtt
    15961 gccgattcag caattacaat tcgttggaga actaatgttt catgtcaaac tgcagttctt
    16021 tatggcaatg atccggattc tttaaatttt tctgtgagtg attctgcaaa tgtaattgat
    16081 catgagataa gattaactgg gctcagtcct gatacacgat atttttatgc ccttggtgat
    16141 ggaacaaatg ttcttcaagg tgattcaaat aattatttta aaacagcacc tgtaaaagga
    16201 atgccaaagc ctgtcaggat ttggtcaatg ggcgatacag gaattaacac cattgatcag
    16261 aataatgttc gcgatgcatt tctgaattat aatgactctt tgccaataga tttgttgtat
    16321 atgcttggtg ataatgccta ttattcaggc acagatcagg agtatcagga tgcatttttt
    16381 cagaatcact atgaagatat tttaaaaaat atacctgttt ttagtgttgc cggcaatcac
    16441 gaaaattata cagcaaatgc aatcacacaa acaggtcctt attatgatat ttttactttt
    16501 ccaaaaaatg gagaatgtgg tggtgtgcct tcaggttcag aagcgtatta ctcttttgat
    16561 tacagcaata ttcatttcat agcattagag actaatacac tttcgctgtt gcagcctgga
    16621 agtgatatgc tcaactggtt gcaaagtgac attgctgcaa cacaacagaa atggaaaata
    16681 gttgtatttc ataactcacc ttacaccaag ggtggtcata actcagatac tgagacagac
    16741 ttaattctga tcagacaaaa tattatgcct attctggatc aacataaagt agatttggta
    16801 ttatgtgggc ataaccatgt gtatgaacgt tcttatttta tggatggaca ctatggttta
    16861 tcgtcaacat ttgattcaac aatggttgta gattcatctt ttggtgaact accccaacca
    16921 tatctaaaaa agtcgtcaaa gaattataaa ggattggttg tagtgcaagc cggatgctcc
    16981 ggtgttttag atcctattgg cccgcaatgg cctcatcctg caatgaaaag atattatgat
    17041 caggaacttg gttcattact tatcgaaata gaaactgata cactaactgt gacattcgtt
    17101 gacaataatg ttagttcacc gcaggtgctg gacgaattta gtgttgttaa agaatgtaat
    17161 gttgttccac aacttacagt tcctgttgca aaagtttgtg ttactgatga tccagtgcaa
    17221 ttgagtgcaa cacctattgg cggatttttt tcaggcctcg gtgtggttga tacagttttc
    17281 aatccaactg ttgccgggca agggatacat acaattgcct atacctataa tgatatttat
    17341 ggctgtcatg ttaccgatac aaaaactatt gaagtagtag gaggagttcc ttcacaacct
    17401 ttcggtattg ttggttctat tttggtttgc cctccttcaa ataatgaagt gttgtcaatt
    17461 caatcattgc ctgatgcgga tagttacatc tggtcagctt ctgccggagt aaatcttact
    17521 tcttcaaata cagatacctt gattaagttt gatataaata cagttcttcc acagtacaac
    17581 atttctgtca gtgctttaaa tatttgcgga agcagtattc cttctttagt caatattact
    17641 tcagttaaat cttttccggt tagtttaaca ggaaatagtg ttatctgccc gggtgatact
    17701 tctgtttata cagtaaatgt tttcaatagc ctttcatcat attggtggca accttctgaa
    17761 cacgtttttt taaacggaca atccgatata atcaatacat ttaatacatt gcatgtgaat
    17821 gcttctgttg acagtagttt taccgaggga aaaatttgtg ttgcaagtaa agaagcatgt
    17881 ctaaggaata atgctgttag ttttattgat ttaagcacaa agccacctgt tcccggtgtt
    17941 atttcaggtc cggctgctgt tttgggtgga agtaatggtg ttgtttacag cattgtgccg
    18001 gttagtggtg caacaggtta tttctggacg gtgacaggtg atgctatact tactgcaggg
    18061 cagttcggca attccgttac cgttgacttc ctcccttcgt ttacaggggg atatgttggt
    18121 gttaaaagcc aaagtaactg tgatgaaagt attgaggttt ctaaacgaat taaaacctat
    18181 attattcagg tttcacagaa tacagtttcg cagacaagaa tagcaaatga atacaatcag
    18241 gataaaattg tttctgtgta tcccaatcca atcggcaatg aaatgcatgt taagttgttg
    18301 ttgcaagatg cctcatcaaa tgttcttgtt gttttaaagg atgttgcagg aaaagaagtt
    18361 ggaaaatgga gtaaaaaggt tttttctgat gaggacgata ttactttgaa tgtttcaggt
    18421 ttaagcagtg gtgtttattt tctatgtgtt actactgaca atatgcagtg gaatggagta
    18481 gtggttaaac aaaatgatca ttgataagtt aagggtaggc tgattgtaac ttttgttcct
    18541 aagcctaatt tgctttgtac ttcaattttt ccattaagaa tttgaacaag ttcagaaata
    18601 ataatataac ctaggccaaa tccttctttg ttgtctaatt gttcatcttc ctcatttaaa
    18661 ggttgcttat ttataagctt agttattcgg gtcaactgtt gttcagacat acctttgccg
    18721 ttatcctcaa tggtaatttc atattgattg ttttgtttgg aataaatttt tataaaacca
    18781 ttttcagtaa attttgtggc attggaaatt atgttatgca aaattattga tagtaagttt
    18841 gagtcggatt gaatgatgtc gtcatgagat atggtgctga tgagcctggt gcctttgttg
    18901 tatgttaagt gcataaattg ctctgtaata tcttctgcca atgctcctat agcaacactt
    18961 gaaatctgtt tttttattcg tttgttttga tgtttaatcc agttcaaaat gttttcagca
    19021 tttgcctgaa gcttttctgt agtgttttct atttcaccta acaattcttt atgtttttta
    19081 ttgtccattt ctatctcttc actcgccccc tttttttgca accattgtaa taaatcttaa
    19141 aggtgttatc aaatcatgtg aaataataga aattagtttg tcttttattt tgatagattc
    19201 tgataattca ttatttgctg tttgtagttc tgaagttcgt aatacaacat ttttttcaag
    19261 tagtatattt tttcgcctta tgttaaaggc ataaattctt gcaacaataa ttattattaa
    19321 aagagcaagt cctaccatcg ctaaccaaaa ccatattgtt tcgtaatatt tattttcaat
    19381 atgaaatgca atagttgctt ctgtataatc tttagaaagt cctgtttgtt tgcgtattgt
    19441 aaatttgtat tctcctgatt taaggttaga aaattctaaa gcagattcgt ttggggataa
    19501 gtaaatccat tgcttattgt atccttctaa tttgtattca cagaataaat tttcaggttc
    19561 tccccagtat gggttagaaa attctattct aacatattga acagaatttg gaacttttat
    19621 attatttgaa gtataataaa tagaatcatc tgcgataaat tggtcaatgc ttattggata
    19681 atctgacctt gggtcttcaa ttttttcagg ataaaaccaa accaatcctt ctacatttgg
    19741 tagtgaaaca tagccattgt taagtcttaa ataattaggg tcacatccac cattaaattc
    19801 agggttactg atgccatctt cttctgaata atgaaaatag gaaacctgat aagttgtatc
    19861 gttaaaatag tggacaagtt cagcaaattt tgttttgata actccattgt tggtagccat
    19921 ccaaagaaac cccaacgaat caggcacaaa attatgtacc tgtcttaaag cttcaaatct
    19981 gccatttggc atttttacag tcttaccgtt tttatacatg taatagccac gcccatatgt
    20041 tccaatcaga agataattgt taatagcttt aaaatgataa gtgtatttca aggcaagttc
    20101 gggcaaggtg tctaacttgc cactttgagt gtcatattta taaataccgg tatagttaca
    20161 tatccaaagt ttaccttcaa atcgtaatag ctgaaaaata ttagaatttg attcatcatt
    20221 gtctaatatt ttgatatgtt ttaatgtatc atttacaaca cagccgaaac ttttgatgag
    20281 cccaacccac atagtgtcgc cctcttcaaa aaaagaaagc ggaagttcgt cccctgtata
    20341 gtagatgtat ttttttgctt tggtttttgg attaaaacga accaatgagt tattctcagc
    20401 ataccaatag ttgccttttt tatcaataca aatgttttcg gaataatttc tagcaaggtt
    20461 gagcttactt tgtttgcctc cattaatggt aaattctctg ttccaatcgg tataaacagt
    20521 gctgtcattt aaggctattt gacaaaaata agtgttgttt gtgccgggtt cgggattggc
    20581 gaactctaag gatttgaaat tacgtttctt gtaaacaaat aagccgcgtg tatctgtccc
    20641 tatcattatc agattctgat cttcattata aatgattgag gaaataagac agttgtttgg
    20701 gagttggttt gtcagttgaa ccgtgctaat caaatttgca ttcaatgagt ccggaaaaaa
    20761 agaatacaaa gtataatcta cctgaacaca agggtcacta ttgttgaaat cccagaatgt
    20821 gttagtgatt ttacgggagt aatttttaca ggaacttaaa tctcctttta atttacattt
    20881 aaccaatgac cacgatgtta aatcaactct atatatttca gaatcatttg cgaaaacata
    20941 aatatttcca ttagcagaaa tgaacttgcg gttctgtaaa tgatctaaat taagctcctt
    21001 aagtttattc ttgcctttaa agtgtgcaat tccttttttt gtcctgacaa tgtatgaatt
    21061 atccggtaag ggaaacatga ttaacggagt taaaatccaa cttttgtcaa cggttatgtt
    21121 tacatgaggt gtggataatt ccagatatgt ttttatgtct ggaagtgttc ctttgatatt
    21181 tatataatta agtgagttga tttttccttt tcttaatgtg tcacttgtta aacctttaat
    21241 ctgaaagata tttccacttg gttctatcat tatcttgtcg gcattggtgg tttggtagaa
    21301 atacaagcca aggtagggcg catctgatgt aaaatgcttt ttgaaattaa tgccgtcaaa
    21361 acgtgctaag ccactgccat acacaaccca aacatatccg ttcttttcat aaaacaaccc
    21421 caatacatta ttatcaggaa gccctttttt aatagtgtaa ttttggatac tgtaactgtt
    21481 ttgtgctgat aatttaaaaa atgtaaaaag aaagacaaga aaaagaactt ttaatttatg
    21541 catatacctt agacaatagg aatgttatag gctcgtgctg agttttgtaa atccataaga
    21601 ttattgacac caatcttatc aaaaattctt gccttaaagg ttgctactgt attggatttt
    21661 aaatcgaagt gtgatgctat ttctttaacg cttttacctt ttagcaatag cagtaagact
    21721 tctgtttcgc gttctgacaa atttgaaaac gggttttcac ttttaccttt ttgtacaccg
    21781 tgctgctgat caaagtattt ttgacttaca tattttctgc ctgtaaagaa tagctccatg
    21841 gcttttacta cttcttcttc gctgctttgt ttgttaagat aacctgatgc accaagatta
    21901 tttaaccgtt tggcaaaaat ttcttccgga ctcattgagt aaatcaggat tggcaacaga
    21961 ggaaattgtt gttttatctc ctgaaaaatt tcaagtaaat tcccatcaga caattgcata
    22021 tctaaaatca gatgtgtgaa attatttttt tcaagtgaat caagaattcc ttttgaagaa
    22081 tctgtttcta caatattgaa tttgccaaaa tgggtttcaa gtaaaaattt taaaccgcgt
    22141 ctgatgataa catggtcatc tgcaatgagg agtgatatat ctgaaaatgc attcataatt
    22201 ttagtgagtt ttatcggata aaagtactca atttttaaat tgtagtgtaa tgtttacaat
    22261 tagtattttt ttaggttctt attttaaaaa acaatgttaa ttattaagta cttacgaaga
    22321 tgttggaagt gcgtagagca acccctacaa attaactcaa attgtttatt ttaatttgtt
    22381 ttttgaaaga agggcagaac tttaaaccaa gtattaagag tgattaaaac caaaacattc
    22441 gcattttctc gtatcaatct tgcttcgggc atacaattgc ttgatggctg gtttttaatt
    22501 aatcttaact atattgttct gaattagtaa agtaaaaaca aaaaatataa ataactaaat
    22561 atcaaacaat gactacttta aattaccaaa acaaaaatcc cttagtacaa ttgcttaggg
    22621 tagcggtgtg ttttttaata acattattgc taaattttaa tttgcaggca caaacaactg
    22681 taaccattgg tgcagggtct caaagtggta cacagtccaa tagtgccgca ggcgattgtg
    22741 gccctatgta tcgttcaact gccagtagtg catttgtgta ttcaagacat cactatttat
    22801 acacgcagtc tgagatttct tctgcaggca ttccatctgg taatttgatt accaaattgg
    22861 catggtacaa tcaaaatggt gccggaacaa attctccatg tatttttcaa atatggatga
    22921 aaaactcagc cttaaccgat gttggagtcg gtggtcaaaa ttggtcaact ttaacttctg
    22981 gctcaacttt ggtttacaac agcagcacac attcagttcc tgccacagtt ggttggcacg
    23041 atattgtttt aagtacacct tttacatata caggtggtgc tatagagatt tctgtaaatt
    23101 ttgatatatc tggtggttca agtccttata caacaaatgg tttctcatgg aacaaagatc
    23161 caatcacagg gcgtacttta tcttatgtgg gtagtgcagc acccggagca acattaccta
    23221 atcttcgtac tgtaagagca caaattcaaa tgacttatca aagtggcgca ccatgtagtg
    23281 ctccacctac agctggatca tctacagcaa caccttcatc ggggctttgt gtgagttcaa
    23341 attttgtgtt aaatttatct ggaaatagta ttggttcggg tcagacatat cagtggcagg
    23401 aatcaacaac ctttggcggc ccttataccg atttaggttc ttcttcaagt tcgcctttac
    23461 ttaatacaac agcaacaaca actaaatact atcaatgcaa ggtaacctgt agcggtaatg
    23521 atcagtatag cacacctgtt ttggttactg ttaatcccag ctttcctgga ggcacttaca
    23581 ccattaattc agctttgcca acaagcgggt ctaacttcca aacttttaat gattttaaga
    23641 gtgcgttaaa ttgtggtatt gctggtgcta ttgttgtgaa tgttgtttcc ggtagtggac
    23701 cttataatga acagatagaa tttggtgaaa tagcaaatgc atcttcaata aatacaatta
    23761 ctattaacgg aaatggaaac gttttaactt ttggagcaac tgtaagtact gcaccgggaa
    23821 cattggtgtt gaacggcaca gattatatga cagttaataa cttaattgtt gaaggtaccg
    23881 gggcaaccta tgccttggtt tgccacttat ggaataatgc aaataataat gcctttaata
    23941 attgtacttt taatgcacct gcaaatggaa ctatcacaac aatggtgcct ttctcaatta
    24001 gtggctcagc tacctctgcc acaacttcag gtaatagcgg tagtaataat gtgataactg
    24061 gttgtaccat gtttagtggt tattataata ctgtacttgt tggtaacagt gggttgccaa
    24121 gtacaggtaa tcaggtgatc aattgcaata tcaatgacca aaatttatat gctacgtatt
    24181 gtttatatca ggatggagtt gttttgagag gcaatacact gtctcgacca acccgtactt
    24241 cgttgggtac ttattatggt atttatatct caaccggatg taccaatgct ttggtagaaa
    24301 aaaatcgtat tcgtaatact tgggcaacaa atacaggttc cacaggaact cagtattcta
    24361 tttattgtac tattgatgca acattaggta atgaaaacaa attttttaat aatgtaattt
    24421 ctgatataga atttaatgga acaatatatg gtatgtatat gtcaggtagc gattattggc
    24481 aggcttatca taatactatt tcattagatc aaactacctc aacagctaca agtacaactt
    24541 atggagtgta tgcaacgggt acaactgcat atgattttag agataatatt gtaacaataa
    24601 gcagaggtgg taccggtaca aaatatggcg tttattacag tactcctgcg gcagctacta
    24661 gtgattataa taacatttat atgaatgcag gtgccggcac taactatgta gcttatagtg
    24721 gcgctccata taccacattg tcggcttacc agatagcctc tggaaaagat gccaatagtt
    24781 caagtgtaga tccattctat cttgctcccg gctcaccaac ttataattat accccaaata
    24841 acggtttgtc taatnnnnnn nnnnnnnnnn nnnnnnnnnn nnntctaatg acacaggtac
    24901 acctgttggg gtaactgatg atattaacgg tgccccacgt tctcttacta ctcctgatat
    24961 aggtgcctat gaattttctc ttggtggact tgatgcaggc atcacatggt ttacaccaac
    25021 aagtccaaca agccccggaa tgaagtcaat tactgttaac attgcaaata cacaggcaca
    25081 gactataaac agcgttcagc tttcttatag tgatggaggc gctccagtta cacaaacatt
    25141 tactgcttta aatctccttt caggtaacaa tacaaatctg acatttacta ctcagtataa
    25201 cttaacttca agtgttacaa tgacagtaga tattcttgca gtaaatggtg ggtcagatgc
    25261 agtttcagga aataacagcg atacctatta tttatgtgtt gcaccatccg gcacctacac
    25321 aattaatagt gcattaccta caggcggaag taactatcag agttttacgg atgcaattca
    25381 ggcattaagt tgtggtatca caggtccggt tgtttataat gtcgttgcag cttcagggcc
    25441 gtataatgag caaattacaa tccctcaaat tgatggcgct tcaacaacga atacaataac
    25501 atttaatggc aatggaaata cattaacatt taatggtaat gcaggcaatc caaatacatt
    25561 ggcattaaat ggtgcagatt attttacgtt taataatctg aatattgaag caaccaatac
    25621 aacctatgcc atagcttgcc acctctattc tggtgccgat tcaaacaaat tcaacaattg
    25681 tacttttacg acttccataa caggaactac cacacaactt gcagcatttg ttattagtgg
    25741 aagcccaact actgccactg gattaggtgt ttccggaaac agtaatattg ttgacagttg
    25801 ccacatggtt ggcggatatt ataacactgt aatttatgga accagttcag ctccatatca
    25861 ggaaaataat gaaatcagga atagtacaat cagtgatttt tatggctatg gtatttataa
    25921 tgtatattgc agaaatacaa ttatcagtgg aaacaccgtt gaacgcttaa accgtactaa
    25981 cacaagtact gtttatgcca tttattgtag taccagttct tataatgtat tggtagaaaa
    26041 gaatcgtgtc agaagattat ttgatgcaca gcaaacaagc acatcatctg cctattgtat
    26101 gtacaatggc ggaagtggtg ctgcaggatt cgaaaacaaa tggattaaca acttagtgta
    26161 tgatataaaa tctaaaggtt cactatatgg aatgtattgt ccaagctata attatgtaca
    26221 tgtctatcat aataccatat cttttgacga tgcctctgct accggcacta catctaccta
    26281 tggtatatat gcttatggta catccactga agtcagaaac aatatcatat ctatcacccg
    26341 tggaggtacc ggaaccaaat actgtttgta ttttagtact aatacagctg taatgagtaa
    26401 taacaatgat ttgtatataa atgcaccttc cggatctaat agcattggat atgcttcagg
    26461 tgcccaagca acattagcaa attggcaagc tgccaatggt ggtgcatggg atcagaacag
    26521 ccaaagtgta aatccaaatt atgcaaatat tgcaatagat aattataaac caaccaacgt
    26581 ggcgcttgat aatattggtg ctgcattggg tgttacagat gatataacag gagcagcaag
    26641 aagtttaact acacctgatc cgggtgccta tgaatttgca gcagccaaca aagacgttgg
    26701 tgtgtcagta tttgtcggtc caaaccctga tggttgtttc accaatgcag aaactattgt
    26761 tgttgaaatt aagaattttg gtgttcaaac aattgatttt tcagttgatc ctgtaacagt
    26821 taattgtgtc atcacaggac ctgttaatac aactgtaagt gcaatattgt caaccggaac
    26881 attagcttca ggtgctacta tgaacgtttc attatcacca actgttgatg gaacagcaaa
    26941 tggtacttat acattgactc cttcaacatc tatggcaagt gatgaagata acacaaacga
    27001 tgctttgact ccgataaata ttactgttgg gccaattgca ggaacagtga gttcttcact
    27061 ttcagatatt tgtgttagcg atgatcctgt tttaacagta acaggacatt atggtggtga
    27121 cattcaatgg cagcaatcaa cagtaggtgc tggtggtcct tggacaaatg ttggaaccaa
    27181 ttctgccacg tattcccctg gaacaattac tcagagtaca tggtatcagg cattgactgt
    27241 ttgtaatagt aatactgcaa cttctaatgt tgtagcagta acagtaaaca atccgcaact
    27301 taacagtctt attcctggaa caagatgcgg ctatggttca gtagcacttg gtattaatgt
    27361 gaacatgggt acaacaggaa attggtatgt tgattcaact agtaatacac caattgcaac
    27421 aggtacaacc tttaatacac caaatctgaa tagtacaact acttattatg ttgctccaag
    27481 ttccggtggc tcaaatggaa tcacaattcc gggtgatggt gattgggatc acgttacaac
    27541 aacaggatct ttccaaacaa cgctaataac tggtgcttac atgattttaa cagtaacgca
    27601 accatttaca cttacctcat tcgatattta tccatcggct gcaatgaaca cagcatttgg
    27661 tattgaagcc agaacaggta gtgcatcagg tcctatgttt atgagttatt ctggtaatac
    27721 aaccgttgta aactcaggtg cccctacagt agcacaaaca gttaatgtaa attgggtttt
    27781 acctattggt acctactata ttggattcgt aggcactaat ccaaattctt ggcgttcagg
    27841 tttggcaaca catagctacc cttgggtgtt gccaggttat gcatcgttag attattatct
    27901 gacaccaaac taccagtatt atttttataa tcttaagatt tctaccggat gcgaaggtgt
    27961 gcgtgttcca attactgcta ccgtaacgtc accaccggca ttaagtgtta taaccgcaga
    28021 cccaacatta tgtcctggaa atactacaac agtgaatgta agcagtatta atgatccgaa
    28081 ttacacttat tcttggacaa gtaatccggc aggatttaca gcatcaggtg caggtcctca
    28141 caatgttagt ccatctgtaa caacacagta tattgtaact gctaccgata atactgtcgg
    28201 ccccaatgca ggttgtgttg ctgtagatag tgttgaggta ataacaggat ctgtattgac
    28261 agcaggaaca gtagcttctt ccgatacaat gttttgtcag tcaggaatac ctacattatc
    28321 agttacaggt gccgatggag gcgctattca atggcagagt tccacagtaa gcgcatctgg
    28381 tccttggaca aatgtgggaa ctaatgcagt agtttatgca cccggtacac ttactcaaac
    28441 tacttggtat caggtaatgg tacattgtca gttaacagag gtttattcaa atgtggttga
    28501 agtaacggta aataatccac aattatcaag cagtaatgga gatacgcgtt gtggaccggg
    28561 tgttgttaat ttaacagcaa gcgcattaag tggtactgtt aattggtaca cgcaatcatc
    28621 cggtggtaca ccaattgcaa caggaaatac ttactctcca actgtaagca acacaactac
    28681 atattacgtt gcagctactt caggtggtgg aacagaatct gttgggcgtc caacttatag
    28741 taacacacca aatacaaatg gtaatgcatg gggtttggta ttcaatgtag tgactacacc
    28801 tattgatctt cagtcagtaa ctgtttattc tgttggagca ggtggaacaa tttctgttca
    28861 gttgaccgat aatgttggta cagtattatc tactgccggt ccatttagtt atccggcagg
    28921 tagtgttgca acaccggttg ctgttgttct tcctgtgaac tttacagttc cggttggtac
    28981 aggttacaga ctgttgtcag cagctttttc aggtggtaat ttgatacgtg agacttcagg
    29041 tatcacatat ccatatactt caccaagtag taatgtggtt attacaagtg gttatataag
    29101 tggaacaacc ttttcaactt actattggtt ttatgattgg cagatatcaa ccggctgtga
    29161 aggagtacgt gtacctattg tagctacagt taatccatca cctgctgtaa atatagctgt
    29221 tcaataccca acattatgta caggtcagag tgcaacatta acagcatcaa gtagtaatcc
    29281 tgattatacc tattcgtgga caccgggtgg aagtggagca agcattgttg tggctcctgg
    29341 cacaaccact acatatactt tgaatgcaat tgataataca gctggcccat atagccaatg
    29401 tactgcaatt gccactcaac ttgtttcagt tgtaaatgcc aatccaccaa taccaattgc
    29461 cagttctgga ggcgatgttt gtttaggtag tgatatattc ttatcaagtg ataacattga
    29521 accaggacaa tttagtggta acacttatga atggacaggc ccaaattcat ttatcagcaa
    29581 tatacaaaat ccatcaatta gtagccctgg agtgtcgtat agtggaactt ataatgttat
    29641 tgttacaaac caatatggtt gtacagaagc agcaagcacc agtctgagtg taaatccaaa
    29701 tccgacttta acaatagatt cagtacagaa tgtaagttgt ttaggcggaa gtgatggtat
    29761 tatttatgta tcagcgtcag gcggcttagc accttatagt tatacttcag attttattaa
    29821 ctttaataca aatggaataa tgaacaatct gccaactgga gcaagtatta tttatgctgc
    29881 agatggcaat gcctgtcagg cacagataac gggtaa
//
LOCUS       DAOOOI010000016        29643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_276_length_29643_cov_21.936393, whole genome shotgun sequence.
ACCESSION   DAOOOI010000016 DAOOOI010000000
VERSION     DAOOOI010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 29643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29643
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_276_length_29643_cov_21.936393"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PKX59_03855"
     CDS             <1..205
                     /locus_tag="PKX59_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ90033.1"
                     /translation="IATAVDIETAMTKGVNYPQGLLTWANKRGLRAVYNKMEELFAYY
                     GEDRYRVCPLLRKLALNDEMFRI"
     gene            284..1558
                     /locus_tag="PKX59_03860"
     CDS             284..1558
                     /locus_tag="PKX59_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOZ90034.1"
                     /translation="MENQEQKSKFQQAIDVITGVAKITIGAGIVHCKAQDEQMDGRYI
                     TLEGQKKLFFGTCGYLGIEKHPKIIEAAVEALHSHGAQFSSSRAYVSSHYYEEAESLF
                     SKMFGRPALVMQSLTLGHITGLPLLVGDNDAIIMDVQAHDSIQSATAMLKLRNVKIDI
                     VRHNRMDILEDRIKVLKNRYQKIWYLGDGVYSMHGDFAPVKELYALADKYEQLHLYLD
                     DIHGMSWTGPHGTGMVMNAVPYYHRKLFLSTGMTKAFGTAGGLLTYPDEEYFKLVKTC
                     GKGFIFSIQLPPAILAATIASCKIHMSDEIIPLQQSLMAKIDYFNKKAEELGLLVIRK
                     ERSPVFFIGVGNPNVGYSTVLRMKKAGFFFNLSVFPAVSIKHTGLRITLSNHIQIEDI
                     DELLETLAENLPVVFKENNTSFEQIKEEFANH"
     gene            complement(1555..2616)
                     /locus_tag="PKX59_03865"
     CDS             complement(1555..2616)
                     /locus_tag="PKX59_03865"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOZ90035.1"
                     /translation="MDSQTRYIPHLDGIRFIAAMMVVFNHYLSELNFDYKFSTGFYGV
                     QIFFAISGFLITGILLRLKNAEKVIPRRLIIKNFFAKRFLRLFPPYYLLIFSYIFLEC
                     FGLYIAQKGWIVFYLAYLPNWFFYFHGWQGTIDNHLWSLGVEEQFYLLWPFVLLLLPK
                     GTEFKWIITMIITGYLFKCVIRQYFMLGGDPFLLPISQMDTLGIGALLACGFEYRMQF
                     IEYFKKALPYLLPLAILLTLVTSYDKPRNDPFLCLSLILLSSSLVLNAVVGFKGVVKI
                     FMENKWVVYGGKISYGIYLYHKVIPTLFISSVERVGIGMPNRWLTLVVSIVLTFLISH
                     LSWKLIESPILKLKKNFEH"
     gene            complement(2765..3190)
                     /locus_tag="PKX59_03870"
     CDS             complement(2765..3190)
                     /locus_tag="PKX59_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012165293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ90036.1"
                     /translation="MNKQLTILLIDDDKVDSMIIKRAIAKLDFPYQVDTAHNGLDALM
                     MLRGMSDIRIKRPDIILLDINMPLMNGLEFLSELRADPELKDIHVCVLTTSEDDSDVK
                     TAYEFNVAGYFIKPLNISDFDDRFLYLNGYWQRNCFPVQ"
     gene            3299..5080
                     /locus_tag="PKX59_03875"
     CDS             3299..5080
                     /locus_tag="PKX59_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ90037.1"
                     /translation="MDTPVVKWIIRKLMLDSIYRSIQDDDILAKQRFNLFRIFNLVAI
                     IGIGLICYQAIYVFQTEKNLAYILLGLESLLILNYLLLNVHKNLTLSVVISLLSCFGA
                     LHYITYFAGGIRNSGMFYQIAFILISFMLLGSRYGIWFTILSVANMIYFFLITEYTNW
                     IDYSFIGETASLINQDYLITGLVSLIVISSMINNLESKKNVVISTITDSRNKLAIINK
                     ELKKLSLVASKTDNAVVITNENGVIEWVNDGFTRITGYQFYDAIDQSLQTFLSGPETD
                     KENLEAVQNSMREFKFCDSELLRYKKDGTTVWMHASITPISDNEKNSRKFIFIMSDIN
                     ERKKAEIQLSEYLSDLEKTNKELDKFAYIVSHDLKAPLRAIGNLTDWITQDSADSLSD
                     ESKENFQIIKGRVKRMEQLINAILEYSKASKHKGTQELFSFNEIVHNAVDLAGADSNC
                     KIEISGAMPEYYGDKVKYQQVFMNLISNGIKHNNKEQKHIEISCHEEGKFWEFKIKDN
                     GPGIDKRYHEKVFVIFQTLKARDEFESTGIGLSIVKKIVEEAGGSIRIESTPGEGSAF
                     FFTVPKNQIDASLITGKNNQQISPLVL"
     gene            5147..5497
                     /locus_tag="PKX59_03880"
     CDS             5147..5497
                     /locus_tag="PKX59_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90038.1"
                     /translation="MKQKQKKSNNLSKVKNFDFDHYLNLRKAERIKLLQQMVEKLDPA
                     SRKNFFKSLLQYVLSDEELTALAFKNNSGKQEVKKSRVVELSDKEKEMLGQIADYFHE
                     LELIMDLDELLINP"
     gene            complement(5512..5997)
                     /locus_tag="PKX59_03885"
     CDS             complement(5512..5997)
                     /locus_tag="PKX59_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOZ90039.1"
                     /translation="MLKFLSIAQSKKRENKQVLKDLSKSKSPQLDNLFREESDNFFKK
                     FSCLDCANCCKTTSPIFRDVDIERIAAHLKLKPGDFVAKYLHLDSDSHYVLNHSPCPF
                     LGTDNYCSIYTVRPQACREYPHTDRKNINQIMDLTYQNTLVCPAVASIVEKLKTDFIK
                     K"
     gene            6178..6681
                     /locus_tag="PKX59_03890"
     CDS             6178..6681
                     /locus_tag="PKX59_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOZ90040.1"
                     /translation="MPKTIFLIRHSKATREYSQINDSDRPLTEKGCHDTEKMSLILKD
                     HINSVDLILTSTAVRAYASALIYSKHLGYHPNNIQLEKNLYMTGSKDMLAILKSLDDQ
                     INSVALIAHNPGLERLVKFLTGTESLHFSTSGLAMIESKIDAWNELNAGNCNLLSYRH
                     PKETIYN"
     gene            6790..9195
                     /locus_tag="PKX59_03895"
     CDS             6790..9195
                     /locus_tag="PKX59_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HOZ90041.1"
                     /translation="MRRKIEKVAVLGSGIMGSRIACHFANAGFNVLLLDIAPKEFTEA
                     DKKAGFVEDSPKFRNKITDTHLQSAIKSNPSPLYHKSFAQRISIGNFTDNLKDIANFD
                     WIIEVIIEDLNIKRTLFEQVEKFRKPGTLITSNTSGIPIHLMTEGRSDDFKKHFCGTH
                     FFNPPRYLPLLEIIPTQFTDKDVIDFISHFGDRYLGKTTVLCKDTPAFIANRIGVFGI
                     MSLFHSMEKTGLSVFAIDKLTGPIIGRPKSATFRTSDVVGLDTLVHVANDAYKNLPDD
                     EQKNLFAIPPFINKMIENKWLGDKTKQGFYKKTKTAEGKTEILELDLTTLEYKPSAKI
                     KFKTLESTKTVDDLKQRLPMLLSGTDEAASFYRYMFFSLFSYVSHRIPEIADEIYKID
                     DAMCAGFGWEIGPFETWDILGVKSVVEQMQKEGYSISPWVTEMISNGINSFYKIDNGK
                     KSYFDLQTKKLNQLPGEGSLVILKNTSNEKIVWKNSGCLLHDIGDGVLNLEFKTKMNT
                     IGSEVLQGINTAIDFAEKDFKGLVIGNEAANFSAGANLGLVFMLAVEQEYDELDYAVR
                     AFQNTNMRLRYSNVPVVVAPAGLTLGGGCEMSLHADKVQAAAETYIGLVELGVGLIPG
                     GGGTKELTLRFSDSIKPGDIETNALRDFFMTIATAKVSTSAFEAYDLHILRKGFDNIT
                     LNRKRLLSDAKNAVLSLANAGYVSPQPRHDIRVLGRQGLGVIQAGASAMLAGNYISQY
                     DQMLSLKLGYIMCGGNLSSPSLVSEKYLLDLEREAFLSLLGEKKTLERIQAILTTGKA
                     LRN"
     gene            9400..10044
                     /locus_tag="PKX59_03900"
     CDS             9400..10044
                     /locus_tag="PKX59_03900"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90042.1"
                     /translation="MKILKQLLLLLILLPAFAFAQREEKPRYAPGREKHRNKTDELGQ
                     RQGVWKYYNEAGELNQEIEYLDDVRNGIAKKYYPYGKIMEEIEYANGIKDGEYKRYYY
                     NGQAKMEGEYLNGKKSGTWTTYFSDGEIRNTGPYKVNLKNGLWKVYDRKGKLISQVEY
                     KMGEDAKALAAAKEADEKAKKAAAAKKAADDKKKLPPGAKLKTPSPPPSTPEKK"
     gene            10102..11277
                     /locus_tag="PKX59_03905"
     CDS             10102..11277
                     /locus_tag="PKX59_03905"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOZ90043.1"
                     /translation="MDAYIVAGYRTAVGKAPRGKFKNTRPDDLAVTVIKHLIQSVPNF
                     DTELIDDVIAGNATPEAEQGLNIGRMISLMGLNTVKVPGMTINRYCSSGLESIAIASA
                     KIHSGLADCIIAGGVECMSPIPFGGWRIVPNYEVAANNPEWYWNMGLTAEAVAKEFKI
                     NREDQDQFAFQSHQRAINAIKNNLFKEQIVPVEINETYLDANEKRQTHKSTVDTDEGP
                     RQDTTIEKLNKLKPVFDAKGTVTAGNSSQTSDGASFVLVVSEKMLKTLNVKPIARFVS
                     YAVSAIEPRIMGVGPVYAIPSVLKRAGLKQNDMELIELNEAFASQSLAVIRTLGLNPE
                     ITNVNGGAIALGHPLGCSGAKLSVQLLNEMKRRKMKYGMVTMCVGTGQGAAGIYELL"
     gene            complement(11284..12894)
                     /gene="bshC"
                     /locus_tag="PKX59_03910"
     CDS             complement(11284..12894)
                     /gene="bshC"
                     /locus_tag="PKX59_03910"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HOZ90044.1"
                     /translation="MFVQNTSIPFADAGYSALITDYLNNNIFLKEYASFQYDESGFKE
                     ALQKRVKYKANRDRLCEVLEQQYSETIFQTDWKTDKSYSAIKSLKEENTFTVTTGHQL
                     NIFTGPLYFIYKILTVIKQAEFLAQRNPGYKFVPVYWMASEDHDLAEVNHIDIETSKI
                     EWQTNQKGAVGRMTTEGMYSVVEALKNQLNKNDNFQELATILDTAYLKNATQANAIRS
                     LVHSLFSKYGLVIIDADDKRFKQQAIKLFEDDLFNNTSFNCFKNLSDFEKAYGLQIHA
                     REINLFYLVNGDRSRIIKNGERFEVADSGLSFSSDELKKHINEYPERFSPNVVLRPLY
                     QEIILPNIAYTGGPAEVHYWLQLKSIFDKLKVFYPMVVLRNGMMVVNADQATVLNESA
                     LEWKDLFHPTEQVVNIALKSKYGEELSLKEEKDTLQRSYEEMEQRFKAIDITLEKHVK
                     AEWRRTEKRLMASEKKLLRAFKKKQVDETCKIRNTVKQLFPNGKLQERKENVFPYLSK
                     YGLQFIDDLYKICEPLPTNFKIAVTAEK"
     gene            complement(12988..13470)
                     /gene="rplQ"
                     /locus_tag="PKX59_03915"
     CDS             complement(12988..13470)
                     /gene="rplQ"
                     /locus_tag="PKX59_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOZ90045.1"
                     /translation="MRHGKKINHLGRTHSHREALLSNLASSLILHKRINTTVAKAKAL
                     RKYVEPLITKSKSDSTHSRRTVFSYLQNKEAVSELFREVAPKVNERPGGYTRILKTGF
                     RPGDAAETCMMELVDFNELMPSSKEKGTAKKAKTTRRGRKPKAKADAAGSETTEATEE
                     "
     gene            complement(13496..14488)
                     /locus_tag="PKX59_03920"
     CDS             complement(13496..14488)
                     /locus_tag="PKX59_03920"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOZ90046.1"
                     /translation="MAILAFQRPDKVIMNHSTETFGQFEFRPLEPGYGITVGNSLRRI
                     LLSSLEGHAITNIKISGVDHEFSTIKGVVEDVTEIILNLKQVRFKKQIEGTDSEKVQV
                     VVNGKTEFTAGDIAKHTSAFQVLNPELVICQMDPSVKLVIDMTIDKGRGYVPAEENKS
                     NSHPLGTVAIDSIYTPIKNVKYAVENYRVEQKTDYEKLVLEITTDGSIHPKEALREAA
                     EILIHHFMLFSDEKITLDTKSKTPVEEFDENTLHMRQLLKSKLVDMDLSVRALNCLKA
                     ADVETLGDLVSFSKTDLLKFRNFGKKSLTELEELVRSKNLTFGMNIQKYMLDKE"
     gene            complement(14553..15158)
                     /gene="rpsD"
                     /locus_tag="PKX59_03925"
     CDS             complement(14553..15158)
                     /gene="rpsD"
                     /locus_tag="PKX59_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOZ90047.1"
                     /translation="MARYIGPKTKIARKFREPIFGPDRYFEKRSYPPGMHGAAKKRAK
                     QSEYAIQLAEKQKVKYTYGMLERQFARFYSIALRKEGITGENLLKLIESRLDNVVFRL
                     GIAPTRAAARQLVGHCHITVNGKVNNISSYILRPGDVVGVRERSKSFEAITDSLASRS
                     NKYGWLEWDTTAMQGKFVTTPAREEIPENIKEQLIVELYSK"
     gene            complement(15206..15610)
                     /gene="rpsK"
                     /locus_tag="PKX59_03930"
     CDS             complement(15206..15610)
                     /gene="rpsK"
                     /locus_tag="PKX59_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOZ90048.1"
                     /translation="MAKQQQASAAKTAKKKVVKVEATGEAHINATFNNIIISLTNLQG
                     QVISWSSAGKMGFKGSKKNTPYAAQLAAADCAKTAFDLGLRKVKVFVKGPGAGRESAI
                     RSLNASGVEVSEILDLTPIPHNGCRPPKRRRI"
     gene            complement(15663..16040)
                     /gene="rpsM"
                     /locus_tag="PKX59_03935"
     CDS             complement(15663..16040)
                     /gene="rpsM"
                     /locus_tag="PKX59_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOZ90049.1"
                     /translation="MARIAGIDLPRNKRGEIGLTYIFGIGRSTAQQILDECGISYDKK
                     VDQWNDDELNKIRSFINDNLKIEGALRSETQLNIKRLMDIGSYRGVRHRLGLPVRGQR
                     TKNNCRTRKGKRKTVANKKKVTK"
     gene            complement(16086..16202)
                     /gene="ykgO"
                     /locus_tag="PKX59_03940"
     CDS             complement(16086..16202)
                     /gene="ykgO"
                     /locus_tag="PKX59_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOZ90050.1"
                     /translation="MKVKPSLKKRSVDCKIVRRNGKLYVINKKNPRFKQRQG"
     gene            complement(16236..16454)
                     /gene="infA"
                     /locus_tag="PKX59_03945"
     CDS             complement(16236..16454)
                     /gene="infA"
                     /locus_tag="PKX59_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOZ90051.1"
                     /translation="MAKQTSIEQDGTITEALSNAMFRVELENGHEIIAHISGKMRMNY
                     IKILPGDKVKIEMSPYDLTKGRITYRYK"
     gene            complement(16468..17292)
                     /gene="map"
                     /locus_tag="PKX59_03950"
     CDS             complement(16468..17292)
                     /gene="map"
                     /locus_tag="PKX59_03950"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOZ90052.1"
                     /translation="MVQIEYRSKEEIDLIRVSSLLVGKTIAEVAKHLRDGITTGELDK
                     IAEDFIRSHEAVPAFKGYHGFSGSLCISVNAEVVHGIPGARVLHPGDIVSLDCGVVKN
                     GYYGDSAYTFAIGEVDQQAVKLLQVTRECLYLGIEKAVAGNRMGDVSSNIQKHAEANG
                     FGVVRELVGHGIGKKLHEKPEVPNYGKAGQGLLLKPGLVIAIEPMINMGTKNVKQLND
                     GWTIVTADQKPAAHFEHTIAVDNVKAEILSSFEEIERSEKENLNLYKIDYKQHIIA"
     gene            complement(17301..18662)
                     /gene="secY"
                     /locus_tag="PKX59_03955"
     CDS             complement(17301..18662)
                     /gene="secY"
                     /locus_tag="PKX59_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOZ90053.1"
                     /translation="MKELIQTLRNIFKIEELRTKLLNTLFFIAIYRLASHIVLPGVDP
                     QQLGNLKNQASQGIFGLLDMFAGGAFSQASIVALGIMPYISASIVVQILGIAVPYFQK
                     MQKEGESGRNRLNQITRWLTVLVVAGQAPGYLVNLRAQAQSAIVAYSNPDAMSPTLWW
                     ITSIIILIAGTLFVMWLGERIQEKGIGNGISLLIMVGIIARLPFALRDEFLSRLNQTG
                     GGLIMFLVELVALFAVVVISIMLVQGTRKIPVQFAKKIIGNRQYGGVRQYIPLKVNAA
                     GVMPIIFAQAIMFIPATVAQFFPESSSMQGIAHSLTDFHSVWYNVTFAILIILFTYFY
                     TAITVNPNQMADDMKKNGGFIPGIKPGKQTASFIDDVMSKITLPGSIFLAIVAILPAF
                     ATILGTSTNFAQFYGGTSLLILVGVILDTLQQIESHLLMRHYDGLMKSGRIKGRTSIN
                     APV"
     gene            complement(18662..19114)
                     /gene="rplO"
                     /locus_tag="PKX59_03960"
     CDS             complement(18662..19114)
                     /gene="rplO"
                     /locus_tag="PKX59_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015334416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOZ90054.1"
                     /translation="MNLSNLKPAKGSTKKSKRIGRGEGSGHGGTSTRGHKGAQSRSGY
                     SRKFGFEGGQMPLQRRVPKSGFKNFFRVEYTGINLDVLQKLNEKFGLTEIDHSILCEK
                     GLIAKRELVKILGRGELKVKMNVKAHAFSETAKNAIEAAGGTTTIQEK"
     gene            complement(19125..19307)
                     /gene="rpmD"
                     /locus_tag="PKX59_03965"
     CDS             complement(19125..19307)
                     /gene="rpmD"
                     /locus_tag="PKX59_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOZ90055.1"
                     /translation="MGKVIIKQVKSGIDRDKNQKLTLKALGLRKVYHSVEKENTDQIK
                     GMIEKVRHLVEVTEIK"
     gene            complement(19318..19836)
                     /gene="rpsE"
                     /locus_tag="PKX59_03970"
     CDS             complement(19318..19836)
                     /gene="rpsE"
                     /locus_tag="PKX59_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOZ90056.1"
                     /translation="MIATANKRVKPGEIELKDRLVAVNRVTKVTKGGRTFSFAAIVVV
                     GDEKGVVGYGLGKAKEVTDAIAKAIDDAKKNLVKVTVHKTTVPHEQDAAFGGAKVFLK
                     PAAPGTGVIAGGAMRAVLESVGIHDVLAKSKGSSNPHNVVKATITALAMMRDAVAVAN
                     QRGVDLNKVFNG"
     gene            complement(19846..20196)
                     /gene="rplR"
                     /locus_tag="PKX59_03975"
     CDS             complement(19846..20196)
                     /gene="rplR"
                     /locus_tag="PKX59_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOZ90057.1"
                     /translation="MKNSKDSRRLKIRKRIRGKIKGTNEQPRLSVFRSNKQIYAQIID
                     DVAGQTLAAASSAENGAAKGTKVEISTSVGKMIAERATAKGITKVTFDRGGYLFHGRV
                     KALADGAREGGLKF"
     gene            complement(20210..20764)
                     /gene="rplF"
                     /locus_tag="PKX59_03980"
     CDS             complement(20210..20764)
                     /gene="rplF"
                     /locus_tag="PKX59_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOZ90058.1"
                     /translation="MSRIGKLPVAVPSGVQVNVQGNTVSVKGPKGTLSQTIADGITLE
                     MEENKVVVKRSTEQKRHKSLHGLYRSLLANMIKGVSQGYKIEQELVGVGYRASNQGQM
                     LDLVLGHSHHVVFELPTEVKVSTKTERGSNPMIILESHDKQLIGQVAAKIRSLRKPEP
                     YKGKGIKFVGEVLRRKAGKSAAKK"
     gene            complement(20776..21174)
                     /gene="rpsH"
                     /locus_tag="PKX59_03985"
     CDS             complement(20776..21174)
                     /gene="rpsH"
                     /locus_tag="PKX59_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOZ90059.1"
                     /translation="MTDPISDYLTRLRNAVKANHRIVEVPASNTKKEITKILFDKGYI
                     ANYKFEETDNKQGNIKVALKYNPVTKLPAITRLKRVSRPGLRKYVSHDRLPRIINGLG
                     VAIISTSKGIMTDKEARQLHVGGEVLCYIY"
     gene            complement(21205..21474)
                     /gene="rpsN"
                     /locus_tag="PKX59_03990"
     CDS             complement(21205..21474)
                     /gene="rpsN"
                     /locus_tag="PKX59_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOZ90060.1"
                     /translation="MAKESIKARSRKREHLVEKYAAKRAALKEAGDYQALALLPRNSS
                     PVRLHNRCMITGRPRGYMRQFGISRVLFRKMALEGKIAGVTKASW"
     gene            complement(21491..22048)
                     /gene="rplE"
                     /locus_tag="PKX59_03995"
     CDS             complement(21491..22048)
                     /gene="rplE"
                     /locus_tag="PKX59_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOZ90061.1"
                     /translation="MSYTPRLKGKYKKEIISALKNHFQYKSIMQVPKLSKICLSQGIG
                     DAVSDKKIVDACVAEMTAIAGQKAVATYAKKDISNFKVRKGVPIGVRVTLRDEQMYEF
                     LDRLVSVSLPRIRDFRGINNKGFDGKGNYTLGITEHIIFPEINIDKVSKIRGMDITFV
                     TTAKTDNEAMQLLKEFGLPFRTGSN"
     gene            complement(22048..22365)
                     /gene="rplX"
                     /locus_tag="PKX59_04000"
     CDS             complement(22048..22365)
                     /gene="rplX"
                     /locus_tag="PKX59_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOZ90062.1"
                     /translation="MSKLKIKKGDIVKVIAGESKGKQGRVLEVFPETNRASVENVNIV
                     SKHTRPNAQNTQGGIVKKEAPVNVSNLMLIDPKSGKPTRVGRKTEEGKLVRYAKKSGE
                     VIK"
     gene            complement(22383..22751)
                     /gene="rplN"
                     /locus_tag="PKX59_04005"
     CDS             complement(22383..22751)
                     /gene="rplN"
                     /locus_tag="PKX59_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOZ90063.1"
                     /translation="MIQSESRLTVADNSGAKEVLCIRVLGGTRKRYASLGDKIVVTVK
                     NALPAGNIKKGAVSKAVVVRVSKEVRRNDGSYIRFDDNAVVLLNQADELRGTRIFGPV
                     ARELREKQFMKIVSLAPEVI"
     gene            complement(22802..23062)
                     /gene="rpsQ"
                     /locus_tag="PKX59_04010"
     CDS             complement(22802..23062)
                     /gene="rpsQ"
                     /locus_tag="PKX59_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOZ90064.1"
                     /translation="MSTERALRKTKIGVVTSIKMQKTVVVAEEKKFKHPMYGKFINKT
                     KKFHVHDETNACGSGDRVLIMETRPISKSKRWRLVEILEKAK"
     gene            complement(23071..23271)
                     /gene="rpmC"
                     /locus_tag="PKX59_04015"
     CDS             complement(23071..23271)
                     /gene="rpmC"
                     /locus_tag="PKX59_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOZ90065.1"
                     /translation="MKQSIVKDLTTEEVKSRIKEEVDNLQKHKMQHAVSPMDNPMKIR
                     STRKLIARLKTELNKRMSQTSK"
     gene            complement(23291..23713)
                     /gene="rplP"
                     /locus_tag="PKX59_04020"
     CDS             complement(23291..23713)
                     /gene="rplP"
                     /locus_tag="PKX59_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOZ90066.1"
                     /translation="MLQPKKTKFRKQQKMRNRGNATRGSQIAFGSFGIKSLENAQLTS
                     RQIEAARVALTRFMKREGQTWIRIFPDKPVTRKPAEVRMGKGKGAPEYWIAQVKPGTM
                     LFEADGVSVETAKEAMRLAAQKLPVITKFVIRRDYSAA"
     gene            complement(23745..24512)
                     /gene="rpsC"
                     /locus_tag="PKX59_04025"
     CDS             complement(23745..24512)
                     /gene="rpsC"
                     /locus_tag="PKX59_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOZ90067.1"
                     /translation="MGQKAHPIGNRLGIIRGWDSNWYGGKNYSDRLVEDEKIRNYLHA
                     RLAKGGVARIIIERTLKLITATIHTARPGIVIGKGGSEVDKLKEEIKKITKKEVQINI
                     FEIKRPELDAQLVADGVAKQIEARISYRRAIKMAIASSMRMGAEGIKIMCAGRIGGAE
                     MARTEQYKDGRIPLHTFRADIDFAIADAQTSYGKIGVKVWICKGEVFGKRDLSPNVGM
                     NSTPKQNAGGGDRRQERERGGNDRGGRRGGRPRGEKK"
     gene            complement(24513..24953)
                     /gene="rplV"
                     /locus_tag="PKX59_04030"
     CDS             complement(24513..24953)
                     /gene="rplV"
                     /locus_tag="PKX59_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOZ90068.1"
                     /translation="MGARKKIAADARKEEMKSTYIARLNNCPTSPRKMRLVADMIRGK
                     KVDLALGLLKFSKKEAAGNMYKLLLSAINNWQAKNEGVRIEDQPLVVSKVYVDSARQL
                     KRLRPAPQGRGYRIRKRSNHVTLLVDTVNKKVENTKQVTENTEA"
     gene            complement(24953..25225)
                     /gene="rpsS"
                     /locus_tag="PKX59_04035"
     CDS             complement(24953..25225)
                     /gene="rpsS"
                     /locus_tag="PKX59_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOZ90069.1"
                     /translation="MSRSLKKGPFIDPKLEGKVASMSEGKKKTVIKTWSRRSLISPDF
                     VGHTFAVHNGNKFIPVYVTENMVGHKLGEFAPTRTFRGHAGNKDKK"
     gene            complement(25268..26092)
                     /gene="rplB"
                     /locus_tag="PKX59_04040"
     CDS             complement(25268..26092)
                     /gene="rplB"
                     /locus_tag="PKX59_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOZ90070.1"
                     /translation="MAIKKFNPITPGQRFLIAPDHSNITTNEPEKALTERINRKGGRN
                     NSGKMTMRYIGGGHRKLYRIIDFKRNKDGVPGKVATIEYDPNRTARIALVHYADGEKR
                     YILAPTGLTVGQTILSGDKATPEVGNAMMLKDMPLGTIVHNIELRPGQGGKMARSAGS
                     YAQLTARDGKYAILKMPSGETRMVLSTCVATIGSVSNSDHNKESAGKAGRNRWKGRRP
                     RVRGVAMNPVDHPMGGGEGRASGGHPRSRKGLLAKGKKTRKLKNQSNKHIIERRKK"
     gene            complement(26103..26393)
                     /gene="rplW"
                     /locus_tag="PKX59_04045"
     CDS             complement(26103..26393)
                     /gene="rplW"
                     /locus_tag="PKX59_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOZ90071.1"
                     /translation="MSILKKPVITEKMTQKSEKLGQYAFIVDKNANKIEIKAAIEKLY
                     NVNVVSVNTVNNAVKSKTRFTKAGILQGKKGGQKKAYITVAKGETIDFYASI"
     gene            complement(26390..27019)
                     /gene="rplD"
                     /locus_tag="PKX59_04050"
     CDS             complement(26390..27019)
                     /gene="rplD"
                     /locus_tag="PKX59_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOZ90072.1"
                     /translation="MELEIKQKNGQATGRKVTLNSEIFEAKPNDHAIWLDVKSIMANA
                     RQGTHKSKQRNEVSGTTKKLKRQKGTGGARAGSMKSPLFVGGGRVFGPQPRDYSFKLN
                     KKVKRLARISALSYKAKDNNITIVENVAMEAPKTSEVVNILKQLELTNKKVLVVTGDT
                     NKNLFLSSRNLQRSKVLSASEINTYDILNAQNLVLEEGALPVIEKILNK"
     gene            complement(27025..27642)
                     /gene="rplC"
                     /locus_tag="PKX59_04055"
     CDS             complement(27025..27642)
                     /gene="rplC"
                     /locus_tag="PKX59_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOZ90073.1"
                     /translation="MSGIIGKKIGMTSMFNAEGKNIACTVIEAGPCVVTQVKSVATDG
                     YDAVQLAFDDKKEKHTTKALQGHFKKSNSSFKRKVVEFKGFTTEVKLGDVIKVDICPE
                     GEFCDVIGTSKGKGFQGVIKRHNFAGVGGATHGQHNRLRAPGSMGASSFPSRVLPGKR
                     LTGHMGDEQVKIQNLQILKVMPEHNLLIIKGAVPGAKGSYIKVEF"
     gene            complement(27666..27971)
                     /gene="rpsJ"
                     /locus_tag="PKX59_04060"
     CDS             complement(27666..27971)
                     /gene="rpsJ"
                     /locus_tag="PKX59_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOZ90074.1"
                     /translation="MNQRIRIKLKSYDYNLVDKSAEKIVKTVKTTGAVISGPIPLPTE
                     KKIYTVLRSPHVNKKAREQFQLCSYKRLLDIYSSTSKTVDALMKLELPSGVEVEIKV"
     gene            complement(28007..>29643)
                     /gene="fusA"
                     /locus_tag="PKX59_04065"
     CDS             complement(28007..>29643)
                     /gene="fusA"
                     /locus_tag="PKX59_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOZ90075.1"
                     /translation="LQLPIGAEEHFKGVVDLINFKGIVWNEHDQGMTFTEVPIPAEIL
                     EEAKSLREKLLESIAEFDDKLMEKYFDDPNSITEREILDALRKSCIAGKVVPMVCGSS
                     FKNKGVQTMLDLVMELLPSPMDKDNITGTNPDNGEPVSRKPDVKEPFTALAFKIATDP
                     FVGRLAYFRAYSGRLESGSYVLNTRSGNKERISRIFQMHANKQNQIPFIEAGDIGAAV
                     GFKDIKTGDTLCAENAPMVLEAMQFPEAVIGLAIEPKTQVDTEKLGTALNKLAEEDPT
                     FRVKTDEETGQTIISGMGELHLEIIVDRLKREFKVECNQGAPEVSYKEAITGSVSHRE
                     VYKKQTGGRGKFADIQFTLSPVDEDFDPIASKSSLQFVNQISGGNIPREFIPSIEKGF
                     KAAMVNGVLAGYPLSSLKVILTDGSFHAVDSDALSFEIAAKLAYKEACAKAKPILLEP
                     IMKVEVVTPDEYMGDVMGDLNRRRGQMEGMDTRAGAQVIKAKVPLSEMFGYVTTLRTI
                     TSGRASSSMEFSHYSECPRAIAEEVIAKVKGKLEKA"
BASE COUNT         8837 a         5853 c         5059 g         9894 t
ORIGIN      
        1 aattgcaact gctgtggaca ttgaaactgc catgactaaa ggagttaatt accctcaagg
       61 gctgcttact tgggcaaata aacgtgggtt gcgagcggtt tataataaaa tggaagaact
      121 ttttgcctac tatggcgaag accgttatag agtatgccca cttttaagaa aattggcttt
      181 gaacgatgaa atgttcagaa tttaagctga atttgataaa tatcatcagt attttgctat
      241 tacaaacgct atttttgcac gcgaatcatt ttttgggtta gggatggaaa atcaagagca
      301 gaaaagtaag tttcaacagg caatagacgt tattaccggt gttgcaaaaa tcaccattgg
      361 tgccggtatt gtgcattgta aagcacaaga tgaacaaatg gatggccgct atattacttt
      421 agaaggtcaa aagaaattgt tttttggcac ttgtggttat ctgggcattg aaaagcatcc
      481 taaaattatt gaagcagcag tagaagcact tcactcgcat ggcgcacaat tttcttcttc
      541 gcgtgcttat gtatcatctc actactacga agaagctgaa agtctgtttt caaaaatgtt
      601 tggccgtcct gcattggtaa tgcaatcgct tacacttggt catataacag gcctacccct
      661 tcttgttggt gataatgatg caataatcat ggatgttcag gcacatgata gcattcaatc
      721 tgctacagca atgcttaaat tgcgtaatgt taaaatagac attgtccgcc ataacagaat
      781 ggatattctt gaagatagaa taaaagttct gaaaaacaga tatcagaaaa tttggtattt
      841 gggtgacgga gtttattcca tgcatggtga ttttgcaccg gttaaagagc tttatgcact
      901 tgcagataag tacgaacaat tgcaccttta tcttgacgat attcatggaa tgagttggac
      961 aggtccacac ggtacaggta tggttatgaa tgcagttccc tattatcatc gcaaactgtt
     1021 tctttccaca ggcatgacta aggcatttgg tactgccggt ggactactta cctatcctga
     1081 tgaagagtat tttaaattag ttaaaacatg tggtaaagga tttatttttt caattcaatt
     1141 gcctcctgca attcttgctg ctactattgc ttcgtgtaaa atacacatga gtgatgagat
     1201 tattcctttg caacaaagcc tgatggcaaa aattgactat ttcaataaaa aagccgaaga
     1261 attaggttta ttggtcatca gaaaagaaag atctcctgta ttttttattg gagttggaaa
     1321 tcctaatgtt ggatacagta ctgttttaag aatgaaaaaa gcaggctttt tcttcaacct
     1381 atcggtattc cctgctgtta gtattaaaca cacaggatta cgtattacat taagtaatca
     1441 tattcagata gaagatattg atgaactgtt agagaccctt gcggaaaact tacctgtagt
     1501 gtttaaagaa aacaacacca gtttcgagca aattaaagaa gagtttgcca atcattaatg
     1561 ctcaaagttt ttctttagct ttaatatagg cgactcaatt aacttccacg acaagtgcga
     1621 aattaaaaaa gtcaaaacta ttgacactac caatgtcagc cacctgtttg gcattccaat
     1681 tcctactctt tcaactgaac tgataaacaa tgtaggaata accttgtggt ataaataaat
     1741 tccatagctg attttacctc cgtaaaccac ccatttgttc tccataaata tttttacaac
     1801 gcccttaaat cctaccacag catttaatac aaggctactt gaaagtaata tcaggcttaa
     1861 acataaaaat ggatcattac gtggcttatc ataagatgta accaaggtta ataatattgc
     1921 taaaggaagc aaataaggta atgctttttt gaaatattct ataaactgca ttcgatattc
     1981 aaaaccacat gccagcaatg ctccaatacc cagagtatcc atttgtgaaa tgggcaataa
     2041 aaaaggatct cctcctaaca taaaatattg tctgatgaca catttaaaaa gataaccggt
     2101 aataatcatg gtgattatcc atttaaactc agtacccttt ggaagcagca ataatacaaa
     2161 tggccataac aaataaaatt gctcttctac ccctaaagac caaaggtgat tatcaatggt
     2221 tccctgccag ccatgaaaat aaaaaaacca gttgggtaaa tacgctaaat aaaatacaat
     2281 ccaacctttc tgtgcaatgt acaatccaaa acactcaaga aaaatataac tgaatattaa
     2341 aagataatac ggaggaaaaa gcctgaggaa acgtttagca aaaaagtttt tgataataag
     2401 tcgccttgga attactttct cagcattttt cagtcttagt aaaattcctg ttatcagaaa
     2461 tccggagata gcaaagaata tctgcacacc ataaaaaccg gtagagaatt tataatcaaa
     2521 atttagttcg gatagataat ggttaaaaac caccatcata gctgcaataa aacgtattcc
     2581 atccagatgt ggtatataac gggtttggga gtccatcgtt gagattagcc cgccaaaata
     2641 tatcggctaa agttttcaac aataagaatt tggttaatgc aagatatttc aggtcgaaag
     2701 tcgaaagatt ttcaaccaat ttacaaggtc actaaaacaa atgtctttaa aaagaaagag
     2761 agacttattg aaccggaaag caattacgtt gccaatatcc atttaaatat aaaaaacgat
     2821 catcaaagtc actaatattc aatggcttaa taaagtaacc ggcaacatta aactcatacg
     2881 cagtctttac gtcagaatca tcttcagaag tggttaaaac acatacatga atatctttta
     2941 gctccggatc ggcacgtaat tcagataaaa attcaagtcc attcattaac ggcatattaa
     3001 tgtccaacaa aataatgtca ggtcttttaa tcctaatatc actcatcccg cgcagcatca
     3061 ttagagcatc aagaccattg tgggctgtat caacctgata tggaaaatca agtttagcga
     3121 ttgccctctt gattatcatg ctgtcaacct tatcatcatc aataagaagt atcgttaatt
     3181 gcttattcat taattgccaa atatatcacc taaatattta tctgcaaaaa taaaacattt
     3241 tatttgtcag taacgtacaa acaccttaag tttaacacaa ttttctacac aaaccggtat
     3301 ggatacacct gttgtaaaat ggattatcag aaaattaatg ctcgatagta tttatcgttc
     3361 tattcaggat gatgacatat tagctaagca gcgtttcaac cttttcagaa tctttaacct
     3421 tgttgctatc ataggtatag gtttaatctg ttatcaggca atttatgttt ttcaaaccga
     3481 aaagaatctt gcctatattc tattaggctt agagtcactg cttattttga attatttact
     3541 gcttaatgta cataaaaacc tcacactatc agtagtcatt agtctattat cttgctttgg
     3601 tgctttacat tacataacct actttgccgg tggcattcgt aactcaggaa tgttttatca
     3661 gattgctttt atcctcattt catttatgct acttggcagt cgttatggca tttggttcac
     3721 aatactgtct gttgccaaca tgatttattt ctttttgata actgaataca ctaactggat
     3781 tgactatagt ttcattggcg aaactgcatc tcttatcaat caggattatt tgattacagg
     3841 gcttgtcagt ttgattgtaa tcagcagcat gatcaacaat ttagagagta aaaaaaatgt
     3901 agtcatttct accatcacag attcaagaaa caaacttgct atcatcaata aagaacttaa
     3961 aaaactttca ttagtagcca gcaagacaga caatgccgtt gttattacta atgaaaatgg
     4021 tgtcattgaa tgggtcaatg atggttttac acgaataaca ggttatcaat tttatgatgc
     4081 aatagatcaa tcactgcaaa ccttcttaag cggccctgaa accgacaagg aaaatcttga
     4141 ggcagtgcag aacagtatgc gagaatttaa attctgtgac agtgaacttt tgcgttacaa
     4201 aaaagatgga acaacagtgt ggatgcatgc cagtattaca ccaataagcg acaatgaaaa
     4261 aaattctcgc aagttcattt ttatcatgag tgatattaac gaacgtaaga aagcagaaat
     4321 acagttaagc gaatacttaa gtgaccttga aaaaacaaat aaagaacttg acaagtttgc
     4381 atatattgtt tcgcatgatc tgaaagcacc attacgtgcc atcggtaacc tgactgactg
     4441 gattacccaa gacagtgcag acagcctctc agatgaatcg aaagaaaatt tccagattat
     4501 taaaggccgg gtaaaacgaa tggaacaact tatcaatgcc attttggaat actcaaaagc
     4561 ttcaaaacat aaaggcactc aagagctatt ttcatttaat gaaattgtac ataatgctgt
     4621 tgatttagcc ggtgctgaca gtaactgtaa aatagaaatt tcaggtgcaa tgcctgaata
     4681 ttatggcgat aaagttaaat accagcaagt attcatgaac cttatcagta atggtattaa
     4741 acacaataac aaagagcaaa agcatattga aatcagttgt catgaagaag gtaagttttg
     4801 ggagtttaag attaaagaca atggcccggg aattgacaaa cgatatcatg aaaaagtgtt
     4861 tgtaatattt cagacattaa aagcaagaga tgaatttgaa tctaccggta ttggcctttc
     4921 tattgttaaa aaaattgtag aagaggctgg tggcagcatt cgtattgaat ccactcccgg
     4981 agaaggttca gcttttttct ttactgtacc aaaaaaccag attgatgcat ccttaattac
     5041 aggaaaaaac aatcagcaga tttcaccttt agttttgtaa gatttttgtt tttctttttg
     5101 caacctgctt gcatattcat cgtatgaaat ataaaaattc atagcaatga agcagaaaca
     5161 aaaaaaatct aataaccttt ctaaagtaaa aaatttcgac tttgaccatt atcttaactt
     5221 aagaaaagca gagcgtatta aactcttgca gcaaatggtt gaaaaactcg atcctgcttc
     5281 aagaaaaaat ttctttaaga gtttacttca atatgttttg tcagatgaag aacttactgc
     5341 cttagcattt aaaaacaaca gtggcaaaca agaagtaaaa aaatcaaggg tagttgaatt
     5401 gtctgataaa gaaaaagaaa tgcttggaca aattgccgac tattttcatg agttggagtt
     5461 gataatggat ttagatgaat tactcatcaa tccataagaa tcattacatt tctatttctt
     5521 aataaaatcc gtctttaatt tttcaactat tgacgccact gcaggacaca ctaaggtgtt
     5581 ctgataagtt aaatccatta tctgattgat atttttacgg tcagtatgcg gatactccct
     5641 acacgcctgc ggacgtaccg tataaattga acaataatta tctgttccta aaaaaggaca
     5701 tggtgaatga ttaagaacat aatggctatc agaatcaaga tgtaggtatt ttgctacaaa
     5761 atctcccggt tttaatttta aatgtgctgc gattctttca atatcaacat ctctgaaaat
     5821 tgggcttgtt gttttacaac aattggcaca atccaggcaa ctgaatttct tgaagaaatt
     5881 atcactttcc tctctgaata aattatctaa ctgaggcgat ttagacttgg aaaggtcttt
     5941 caaaacctgt ttgttttcac gctttttgct ttgcgctatt gataaaaact tcaacatttc
     6001 ctgtttcatg cacaagcaaa gaaacaaaat agtgtcattt tcttaacttg ttttataccc
     6061 gttcaaaaaa aattaaataa taacttgcaa aaaggcatca tttttgttaa aattgttgca
     6121 tgatttaact aatttaattt tttttaatca ccctatttaa aaatttaact accaactatg
     6181 cccaaaacaa tttttctaat tcgccacagc aaagcaactc gcgagtattc tcaaattaat
     6241 gacagcgata gaccgctaac agaaaaaggt tgtcacgata ctgaaaagat gagtttgatt
     6301 ttaaaagatc acataaactc cgttgattta attttgacaa gtacagcagt cagagcttat
     6361 gcatctgctt taatctatag caaacatctt ggctatcatc ctaacaatat tcaacttgaa
     6421 aagaacctat atatgacagg ttcaaaagat atgcttgcca tattaaaaag cttagatgac
     6481 caaattaact ctgtagctct aattgctcac aatccgggtt tagagcggtt ggtgaaattt
     6541 ttaactggta cagagagttt gcatttttca accagtggtc tagcaatgat tgaatctaag
     6601 attgatgcct ggaatgaact gaatgcaggt aattgcaact tactctcata cagacatcct
     6661 aaagaaacta tttacaatta gttactaatt gacaaaacag ttctgttgtt tttcataact
     6721 ttatctggtt tttacaaacc ttattcgtaa aataacttag gtttgggttt tattttttag
     6781 atagttttta tgagaagaaa aattgaaaaa gtagctgttc tgggttcagg aattatgggt
     6841 tcacgaattg catgtcattt tgcaaatgca ggttttaatg tactgctatt agatattgca
     6901 ccaaaagaat ttacagaagc tgataaaaaa gcaggttttg ttgaagatag tccgaagttt
     6961 cgaaataaaa ttacagatac tcatttacag tcagcaataa aaagtaaccc ttctccactc
     7021 tatcataaaa gctttgcaca aagaatttca atagggaatt ttacagataa tctaaaagac
     7081 attgctaact tcgattggat tatagaagta attattgaag atctaaatat taaacgaact
     7141 ttattcgaac aagtagaaaa attcagaaag cccggaacac taatcacatc aaacacgtcc
     7201 ggaataccta ttcatttgat gacagaaggt cgctctgatg attttaaaaa acatttctgc
     7261 ggcacacact tctttaaccc tccgcgttac cttcctctac ttgaaatcat tcccactcaa
     7321 tttaccgata aggatgtgat tgattttatt tcccacttcg gtgatcgcta cttaggcaaa
     7381 acaactgttc tatgcaaaga cactcctgca tttattgcta acagaattgg tgtatttggt
     7441 atcatgtcat tgtttcattc aatggaaaaa acaggtcttt cagtttttgc cattgataaa
     7501 cttacaggtc ctataattgg aagaccaaag tctgcaacct ttagaacaag tgatgtagtt
     7561 ggattagaca cattggtaca tgtggcaaat gatgcttata aaaatttgcc tgatgatgaa
     7621 cagaaaaatt tatttgcaat tcctccattt atcaataaaa tgattgagaa taaatggctg
     7681 ggtgataaaa ccaaacaagg cttttataaa aaaacaaaaa ctgcagaagg taaaacagaa
     7741 attcttgaat tggatttaac aactttagaa tataagccat cagctaaaat aaaatttaaa
     7801 acattagaaa gtacaaaaac tgttgatgat ctgaaacaaa gacttcctat gttgctctcc
     7861 gggactgatg aagcagcatc attttatcgt tacatgtttt tctccttatt ctcctacgtt
     7921 tcgcatcgca ttcctgaaat tgcagatgaa atttacaaga ttgatgatgc catgtgtgca
     7981 ggtttcggtt gggaaatcgg ccccttcgaa acatgggata ttcttggagt aaaatccgta
     8041 gtagaacaaa tgcaaaaaga aggttatagc atttcaccat gggtgacaga aatgattagt
     8101 aatggaataa attctttcta caaaattgat aatggcaaaa aatcatattt cgatttacag
     8161 actaaaaaat taaatcaact tccgggcgaa ggcagtttag taattcttaa aaacacttca
     8221 aacgaaaaga ttgtctggaa aaattccggc tgcctgttac acgatattgg tgatggtgtt
     8281 ttgaatctgg aattcaaaac caaaatgaat acaataggta gtgaagtatt acaaggaata
     8341 aatactgcaa ttgattttgc agaaaaagat tttaaaggat tagtaattgg aaacgaagca
     8401 gcaaattttt ctgcaggtgc aaatctgggt ttggttttta tgcttgccgt agaacaggaa
     8461 tatgatgaac ttgattatgc tgtaagagca tttcaaaata ccaatatgcg tttacgctat
     8521 agcaatgtac ctgttgtagt tgcacctgcc ggccttacct taggaggtgg ttgcgaaatg
     8581 agtttgcatg cagacaaagt gcaagcagca gcagaaactt acattggtct tgtagagtta
     8641 ggtgtcggac tgattcccgg aggtggtggt accaaagaat tgacacttcg tttttcggac
     8701 tccattaaac ccggagatat tgaaacaaat gcattacgcg atttttttat gaccattgcc
     8761 actgcaaaag tttcaacttc ggcatttgaa gcttacgact tgcatattct tagaaaaggt
     8821 tttgacaaca ttacgcttaa ccgaaaaaga cttttatctg atgctaaaaa tgcagtttta
     8881 tctttagcta atgccggata tgtgagcccg caaccaagac atgatattcg tgttttagga
     8941 agacaagggc ttggcgttat acaggccggt gcaagtgcta tgctggcagg caactacatc
     9001 agccaatacg atcaaatgct ttcgttaaag cttggataca ttatgtgtgg tggcaattta
     9061 agttcgccat ctttagtatc tgaaaaatat ctgcttgact tggaaagaga agcttttctt
     9121 tctttattag gcgaaaagaa aacacttgag cgcattcagg caattttaac tacaggtaaa
     9181 gcgttaagga attaatattt aatcatttgt gtcagcattt taaaattcat gggttttcaa
     9241 caatttacct aaggccatta tttgtttttc ctctgaaaaa cagaaattaa atgagctgtt
     9301 tataaatgaa aaacatgtag aaaaactctg aatttcattc atgttttgat taaaaatatt
     9361 ttattttagc catttgaatt accataccaa tcataaaaga tgaaaatatt aaaacaactg
     9421 ttgttgttgt taatcctttt accggctttt gcttttgctc aacgcgaaga aaaaccaaga
     9481 tatgcgcccg gaagagaaaa acatcgcaac aaaacagatg aattaggaca gcggcaaggt
     9541 gtttggaaat attataatga agccggtgaa ttaaatcagg aaattgaata tttagatgat
     9601 gtgagaaatg ggatagcaaa aaaatattat ccctatggaa agataatgga ggagatagag
     9661 tatgccaatg gaataaaaga tggagaatac aaaagatatt attacaatgg gcaagctaag
     9721 atggaaggtg aatatttaaa tggtaaaaaa agtggaacat ggacaacata tttcagtgat
     9781 ggtgaaatac gaaatacagg tccatataaa gtaaatctca aaaatggttt gtggaaggtt
     9841 tatgacagaa aaggtaaatt aatcagtcag gttgaatata agatgggtga ggatgcaaaa
     9901 gcactggcag ctgctaagga agctgatgaa aaggctaaaa aggctgcagc agctaaaaaa
     9961 gcagctgatg ataaaaagaa attacctcca ggggctaaat taaaaacacc ctcaccacca
    10021 ccttcaacac cggaaaagaa ataaatttct tttgtaatta gaacctgcta ccaacactaa
    10081 tttattaaat agcaagaaca aatggatgca tacatcgtag ccggatatag aactgctgtt
    10141 ggcaaagcac caagaggaaa atttaaaaat acaagacctg atgatcttgc agtaactgtt
    10201 ataaaacatc tcatacaatc agttccgaat tttgatacag aattaattga tgatgtaatt
    10261 gcaggtaatg ccacacccga agcagaacaa ggcctcaata tcggaagaat gatttctttg
    10321 atgggactta atacagttaa agttcctggt atgaccatta accgctattg ttcatcaggg
    10381 ctcgaatcaa ttgctattgc ttctgcaaaa attcatagcg gcctagctga ttgtattatt
    10441 gccggtggtg ttgaatgtat gtcgcctatt ccgtttggcg gatggaggat tgttcctaat
    10501 tatgaggtgg cggcaaataa tcctgaatgg tattggaaca tggggttaac tgcagaagca
    10561 gttgcaaaag aatttaaaat taatcgtgaa gatcaggatc agtttgcctt tcaatcacac
    10621 caaagagcaa ttaatgccat aaaaaataat ttgtttaaag agcagattgt tcccgttgaa
    10681 atcaatgaga cctatcttga tgccaatgaa aaacggcaga cacataaaag tactgttgat
    10741 actgatgaag gaccacgtca ggatactaca atagaaaaat taaataaatt aaagcctgtc
    10801 tttgatgcta aaggaactgt tacggccggt aactcatctc aaaccagtga cggtgcatca
    10861 tttgtacttg tagtgtctga aaaaatgctc aaaacgctta acgtaaaacc aattgcacgt
    10921 tttgtttctt atgctgtcag cgccattgaa ccgcgtatta tgggcgtagg accagtttat
    10981 gctattccat cagttctgaa acgcgcagga ctcaagcaaa atgatatgga attaatagaa
    11041 ctcaatgagg cttttgcatc acaatcgctg gctgttataa ggaccttagg attaaatccg
    11101 gaaattacca atgtaaatgg tggtgcaatt gcattaggac atcccttagg atgttcagga
    11161 gccaaactta gtgttcaact tcttaatgaa atgaaaagaa gaaagatgaa gtatggcatg
    11221 gtcaccatgt gcgttggtac aggtcagggt gctgcaggta tttacgaatt actttaagtt
    11281 tttttatttt tctgctgtta ccgcaatttt gaaatttgtt ggtagtggtt cacaaatttt
    11341 atacaaatca tcaataaact gcaatccata cttgcttaaa taaggaaaca cattttcttt
    11401 tctttcttgc aatttaccat ttgggaacaa ttgtttcact gtatttctga ttttacaagt
    11461 ttcatcaacc tgctttttct taaatgcacg taaaagtttt ttctctgaag ccatcaaccg
    11521 tttttctgtt cgcctccact ctgccttaac atgtttctca agtgttatat caattgcttt
    11581 aaaacgttgc tccatttctt catagctcct ttgcaaggta tccttttcct cctttagact
    11641 tagttcttca ccatacttgc ttttcaatgc aatattcaca acttgttctg ttggatgaaa
    11701 caaatccttc cattccaaag ccgattcatt caaaacagta gcttgatctg cattcactac
    11761 catcatacca ttacgaagca caaccatggg ataaaaaact ttcagcttat cgaaaataga
    11821 ttttaattgc aaccagtaat gtacttctgc agggccgcca gtatatgcaa tgtttggtaa
    11881 aattatttcc tgatataatg gccgtagtac aacatttggg ctaaatcgtt caggatactc
    11941 attgatatgc tttttcaatt catcacttga gaaagataaa cctgaatctg caacttcaaa
    12001 tctttcgcca tttttaatta ttctgctacg gtctccattg actaaataaa acaaattaat
    12061 ttctcttgca tggatttgta atccatacgc tttttcaaaa tcagaaagat ttttaaaaca
    12121 attaaatgat gtgttattga acaaatcgtc ttcaaacaat ttaattgctt gttgcttgaa
    12181 tcttttatca tctgcatcta taatcaccaa accatatttt gaaaacaaag aatgtacaag
    12241 gcttctgata gcattagcct gcgttgcatt ttttaaatat gctgtatcaa gtatcgttgc
    12301 taactcctga aaattatcat tcttatttaa ctgatttttc aatgcttcaa caacagaata
    12361 cattccttca gtagtcattc tgccaacagc acccttttga tttgtttgcc attctatttt
    12421 cgatgtttca atatctatat gattcacttc tgcaaggtcg tgatcttcac tggccatcca
    12481 ataaacaggt acaaatttat aaccgggatt tctctgagcc agaaattctg cctgcttaat
    12541 tacggtaagt attttataaa taaaataaag tggaccggta aaaatgttca gttggtgacc
    12601 agtagtgact gtaaaagtat tctcttcttt caaagattta attgcagaat atgatttatc
    12661 cgtcttccaa tctgtttgaa aaattgtttc tgaatactgc tgctctaata cttcgcaaag
    12721 cctgtcacgg ttggctttat actttactcg tttctgtaat gcctctttaa aacctgattc
    12781 atcatattga aatgatgcat attcttttaa aaaaatattg ttgtttaaat aatcagtaat
    12841 taatgcagaa tatcctgcat cagcaaaagg tatagaagta ttttgaacaa acatatttgt
    12901 gctatcagag aaaatcactg taaacataaa aataaaaaaa gggatgccga aaccgacatc
    12961 ccctttagat taatttttct gttgaattta ttcttccgtt gcttccgttg tctcgcttcc
    13021 tgcagcatca gccttagctt ttggcttacg gcctctgcgt gtagtcttag ctttcttagc
    13081 tgtacctttt tcttttgatg aaggcatcaa ctcattgaaa tcaactaact ccatcatgca
    13141 agtttcagca gcatcaccgg gtctgaatcc tgttttcagg atacgggtat atccgcccgg
    13201 acgctcgttt acttttggtg caacttctct gaaaagctca ctcaccgctt ctttattctg
    13261 cagatagcta aaaactgttc tgcgcgaatg tgtgctgtca gattttgact tggtaattaa
    13321 tggttcaaca tattttctaa gtgccttcgc tttggcaaca gttgtgttta ttctcttatg
    13381 cagaatcaat gatgaagcta agttagagag taatgcttct ctgtggctat gtgttctgcc
    13441 taaatgattt atcttttttc cgtgacgcat tttacgtaat ctttaaacta ttcaattatt
    13501 ctttatcaag catatacttc tgtatgttca taccgaaggt aaggtttttg ctgcgaacca
    13561 attcttctaa ctctgtaagt gatttctttc cgaagttacg gaacttcaac aagtctgttt
    13621 tcgaaaacga aaccaaatca cccaaagttt caacatcggc tgccttcaga cagttcaatg
    13681 ctcttacaga aagatccata tccacaagtt tcgattttag caattgacgc atgtgaagtg
    13741 tgttctcatc aaactcttca accggagtct tagactttgt atcgagtgtt attttctcat
    13801 cgctgaaaag catgaagtga tgaatcagaa tttctgctgc ttcacgtaat gcttctttag
    13861 gatgaatact tccatcggtt gtgatttcaa gtaccaattt ttcgtaatcg gttttttgtt
    13921 ccacacgata gttctctact gcatatttta cattctttat aggagtgtaa atagaatcaa
    13981 ttgcaacagt tcctaatgga tggctgtttg atttattttc ttctgcaggt acatatccac
    14041 gtcctttgtc aatggtcata tcaataacca attttacaga aggatccatt tggcaaataa
    14101 ccaattcagg atttaatacc tgaaaagcag aggtatgttt tgcaatatca ccggcagtaa
    14161 attctgtttt accgtttact acaacctgaa ctttttcaga gtctgtacct tcaatttgct
    14221 ttttaaaacg aacctgctta aggtttagga taatttcagt tacatcttca acaactcctt
    14281 ttattgttga aaattcatga tcaactccac tgattttaat attggtgatt gcatgtcctt
    14341 cgagtgaaga aagcaatatt ctacgtagag aattaccaac agtaatacca taacccggct
    14401 ccaaaggtct gaattcaaac tgaccgaatg tttcggtaga atgattcata atcactttgt
    14461 cgggacgttg aaatgcaagt atggccattt tatattttgt gttttatttg tttaattatt
    14521 tactgagttt tactgttcag ggttagcaag aattatttag agtacaattc cacgataagt
    14581 tgctccttaa tattctcagg aatttcttcg cgtgcaggtg tagtcacaaa tttaccttgc
    14641 atagctgtgg tgtcccactc taaccaaccg tacttattag aacgagatgc aagagaatct
    14701 gttattgctt cgaaagactt tgaacgctca cgaacgccaa caacatcacc cggacgtaag
    14761 atgtatgatg aaatattgtt caccttacca tttactgtaa tatggcaatg tccaactaac
    14821 tgacgtgcag cagcacgtgt tggtgcaatt cctaaacgga aaacaacatt atcaagtctg
    14881 gattcaataa gcttcaataa gttttcgcct gtaatgcctt ctttgcgaag tgcaatactg
    14941 tagaaacgag caaactgacg ctccaacatt ccgtaagtat atttcacctt ttgcttttca
    15001 gctaactgaa ttgcatattc actttgcttt gcgcgctttt tagctgcacc atgcattccg
    15061 ggaggataac ttcttttttc gaaatatctg tcaggtccga atataggctc gcgaaactta
    15121 cgtgctattt ttgtttttgg tccaatgtaa cgtgccattg tattattcta atattttaat
    15181 ttttaaactg atttgtgata ataaattata tccttcttct tttaggagga cgacatccat
    15241 tgtgtggtat tggagtaagg tcaagaattt cactaacttc tactccggaa gcattgagtg
    15301 agcggattgc agattcacga cctgctcccg gaccttttac aaatactttc acttttctta
    15361 atcctaaatc gaaagctgtt tttgcacagt cagccgcagc aagctgtgca gcataaggtg
    15421 tattcttctt tgaaccttta aaacccattt tacctgcact tgaccaagag ataacctgtc
    15481 cttgcagatt tgtaaggctt ataataatgt tattgaaagt tgcattgata tgcgcctcac
    15541 cggtcgcctc aacttttacc accttctttt ttgctgtttt tgcagctgat gcttgttgtt
    15601 gtttagccat tattcttttt atttattctg aatgctgatt ttcaggattc aatataatct
    15661 gattatttag ttaccttttt cttgttagca actgtcttac gtttaccttt acgtgtgcgg
    15721 caattgtttt ttgttctttg tccgcgcaca ggcaatccta aacgatgacg aacaccacgg
    15781 tagctaccga tatccatcag acgtttaatg ttcaactgtg tttctgaacg taaagcacct
    15841 tcaattttca gattgtcatt aatgaatgaa cggattttat tcagttcatc gtcattccac
    15901 tgatctactt tcttatcgta cgaaattccg cactcatcaa gaatctgttg tgctgttgaa
    15961 cgtcctatac caaaaatata agtaaggcct atttcacctc ttttgtttct tggtaaatct
    16021 atacctgcta ttcgagccat ttgatattaa ctattttctt tgttacaatt atttttaaga
    16081 tgtaattatc cctgacgctg tttaaagcgt gggttctttt tattaattac atacaattta
    16141 ccgttgcgtc taacgatttt acaatcaacg cttcttttct ttaaacttgg tttaactttc
    16201 atctccttaa tttttattct gattcgttac ttcagttatt tataacgata agttattctt
    16261 ccttttgtca gatcatacgg tgacatttca atctttactt tatcaccggg cagaatttta
    16321 atatagttca ttctcatctt tcctgaaatg tgggcaatta tctcatgtcc gttttccagt
    16381 tccactctga acatagcatt gctcaacgct tctgtaatcg ttccgtcttg ctctattgat
    16441 gtctgctttg ccattaatct aatcttacta tgctataata tgctgcttat aatctatttt
    16501 atataaattc aaattctctt tttcacttct ttctatctct tcaaatgaag acagtatttc
    16561 tgcctttaca ttatccactg caatggtatg ttcaaaatgt gcagcaggtt tttgatctgc
    16621 tgttacaatt gtccatccat cattgagttg cttcacattc tttgttccca tgtttatcat
    16681 aggttcaatt gctattacca accccggttt caacaacaat ccttgccctg ccttaccata
    16741 atttggtact tcgggcttct catgaagttt ctttcctatg ccatgtccta ccaattcgcg
    16801 aacaacacca aatccatttg cctctgcatg tttttggata ttgctgctta catcacccat
    16861 tctgttaccg gcaactgctt tctctattcc aagataaagg cattcgcgtg taacctgcag
    16921 tagttttact gcctgctgat ctacttcacc gatagcaaag gtgtaagccg aatcaccata
    16981 atatccgttt ttaactacac cgcaatcaag tgacactata tctccaggat gcaaaactct
    17041 tgctccggga attccgtgaa ccacttcggc attaaccgat atacataacg aaccactgaa
    17101 tccatgatag cctttaaaag caggcacagc ttcatgagag cggataaaat cttctgcaat
    17161 cttatctaac tctccggtag ttattccgtc tcgcaggtgt ttggcaactt ctgcaatggt
    17221 ttttccaaca agtaaagaac taactctaat taaatctatc tcctctttgg agcgatattc
    17281 aatctgcaca attttatatt ttaaacaggt gcattaatgg atgtgcgtcc tttaatacgt
    17341 cctgatttca ttaatccatc gtaatgtctc atcaataaat gactttctat ctgttgtaag
    17401 gtatctagta ttacaccaac aagaatcaac aatgatgtac caccataaaa ttgagcaaag
    17461 ttggtactgg tacctaatat tgttgcgaat gcaggtaaga tggcaactat cgcaagaaat
    17521 atagagcccg gcaaagtaat tttcgacata acatcatcaa taaatgatgc tgtttgtttt
    17581 ccgggtttaa taccgggaat aaatcctcca tttttcttca tgtcatcagc catctgatta
    17641 ggattaactg taatggcagt gtagaagtag gtgaataata taatcagtat tgcgaaggta
    17701 acattatacc atacagaatg gaaatctgtt aatgagtgtg caataccctg catggaagaa
    17761 gattcaggaa agaactgtgc tactgttgca ggaataaaca taattgcctg cgcaaaaatg
    17821 attggcatta caccggcagc atttactttt aaaggtatgt actgacgtac cccaccatat
    17881 tgtctgttac caattatttt cttagcaaat tgcacaggta ttttacgtgt accctgtacc
    17941 aacataatac ttatcacgac cacagcaaac agtgctacta attcgactag gaacataatt
    18001 aatcctccac ctgtctgatt gagtcgagat aaaaactcat cgcgtaaggc aaatggtaat
    18061 cgggcaataa taccaaccat gataagtaat gaaataccat ttccgattcc tttttcctga
    18121 atacgttcac caagccacat cacaaacaaa gtgcctgcaa taagaatgat gatagaagta
    18181 atccaccaca acgttggtga cattgcatca gggtttgaat aggcaacaat agcagattgt
    18241 gcttgtgctc taagatttac taaataccct ggtgcctgac cagccacaac taaaactgta
    18301 agccagcgtg ttatttgatt caaacgatta cgtccactct ctccttcttt ctgcattttc
    18361 tggaagtaag gaacagcaat tcctaaaatc tgaacaacga ttgaggctga gatatagggc
    18421 ataattccca atgcaacaat tgacgcctgt gaaaaggcac ctccggcaaa catatctaat
    18481 aagccaaata taccctgcga agcctggttc tttaaattac caagttgctg aggatctaca
    18541 ccaggcaaca caatatgaga ggccaagcgg taaatggcaa taaaaaacaa ggtattgaga
    18601 agctttgttc ttagctcttc aatcttgaat atgttcctta atgtttgaat cagttctttc
    18661 attatttttc ttgaatggtg gttgtgcctc cagcggcttc aatagcattt tttgcagtct
    18721 ctgaaaatgc atgtgctttt acattcattt ttacttttaa ctcgccacgt ccaagtattt
    18781 ttactaactc acgttttgcg attaatcctt tttcgcaaag aatgctatga tcaatttctg
    18841 ttaaaccaaa tttttcgttt aacttctgca acacatctaa attaatgcct gtgtattcaa
    18901 ctctgaagaa gtttttaaat cctgatttag gaacccttct ctgaagtggc atctgcccac
    18961 cttcaaaacc aaatttgcgt gaatatcctg agcgtgattg tgctccttta tgaccacgtg
    19021 tagatgttcc accatgacca gagccttcac cacgacctat tcttttgctt ttctttgttg
    19081 aacctttggc aggttttaaa ttacttaaat tcataacgct tttcttattt gatttctgtt
    19141 acctcaacca gatgtctaac cttttcgatc attcctttta tctgatcagt gttttctttt
    19201 tctacagaat gatatacttt tcttaaacct aaagctttta gtgttagctt ctgattttta
    19261 tcgcgatcta ttccgctttt tacttgtttg ataattactt ttcccatgag tataatatta
    19321 cccgttaaat actttgttta aatctacacc acgctgatta gccactgcta cagcatcacg
    19381 catcatagct aaagcagtaa tggtagcttt taccacgtta tgcggattag aagatccttt
    19441 tgacttcgct aacacgtcat gtatgcctac actttcaaga actgcacgca tggcaccacc
    19501 tgcaattaca ccggtaccgg gagcagctgg ctttaaaaat acttttgcac caccgaaagc
    19561 agcatcttgc tcatgaggaa cagtagtttt atgaactgta accttcacta agtttttctt
    19621 tgcatcgtca atagctttgg caattgcatc ggtaacttct tttgcttttc ctaaaccata
    19681 acctacaaca cctttctcat caccaaccac aacaatggct gcaaaagaaa atgttcttcc
    19741 acctttagta accttagtta ctctgtttac tgctacgagc ctgtctttta attcgatttc
    19801 gcccggttta actcttttat ttgctgttgc tatcattgtt acgttttaga attttaatcc
    19861 accttctctt gctccatcgg ccaatgcttt tacacggcca tgaaacaaat atccacctct
    19921 gtcgaatgtt actttggtaa tacctttggc tgttgctctt tcagcaatca ttttgccaac
    19981 tgaagttgaa atctcaacct tagttccttt tgctgcacca ttttctgctg atgaagctgc
    20041 agccaatgtc tgacctgcaa catcatcaat tatctgtgca taaatctgct tattacttct
    20101 gaaaacagac aatcttggct gctcattagt acctttgatt tttccgcgaa tacgtttacg
    20161 gattttaagc ctgcgtgaat cttttgaatt cttcattttc ttactttact tattttttag
    20221 cagctgattt tccagctttt cttcttaata cttcgcccac aaacttgata ccttttcctt
    20281 tgtatggttc aggcttacgg agtgatctta ttttagcagc aacctgcccg ataagttgtt
    20341 tatcgtgact ttcaagtatg atcataggat tgctaccacg ctctgtcttt gtgcttactt
    20401 taacttctgt cggcagttca aaaaccacat ggtgtgagtg accaagaacc aaatcaagca
    20461 tctgaccctg attgctggca cggtaaccta cacccactaa ctcctgctct attttatagc
    20521 cttgcgatac tcctttaatc atattggcaa gcaatgaacg atacaagcca tgtaatgact
    20581 tatgacgttt ttgctctgtg ctgcgtttta caacaacttt attttcttcc atttctaaag
    20641 taatgccatc ggctatggtt tgtgataagg tgccttttgg accttttaca gaaactgtat
    20701 ttccttgtac attaacctga acacctgatg gtactgcaac cggtaatttc cctattcgtg
    20761 acatgctttc ttaaattaat aaatataaca taaaacctca ccaccaacat gaagctgacg
    20821 tgcttctttg tcagtcataa ttcctttgct tgtagagata attgctactc caagaccatt
    20881 gataattctt ggcaaacgat catgactaac atactttctc aaacctggac ggctaacacg
    20941 cttaaggcgc gtaattgccg gcaatttggt tacaggattg tattttaaag caactttaat
    21001 atttccctgc ttattgtcag tttcttcaaa cttataattt gcaatatacc ctttgtcaaa
    21061 aagtattttg gtaatttctt ttttagtatt tgatgcaggt acttctacga tgcggtgatt
    21121 ggcctttacg gcattacgca aacgtgtcaa ataatctgat atcggatcgg tcattttaat
    21181 tatttttcta attattgttt aatattacca actggctttt gttactccgg caatttttcc
    21241 ttctaaagcc attttgcgaa acagtacgcg tgaaattcca aactgacgca tataacctct
    21301 tggacgacct gtaatcatac aacggttatg taaacgaaca ggtgatgaat tacgaggcaa
    21361 caatgccaat gcctgataat cgccagcttc ttttaatgct gcacgtttag ctgcatactt
    21421 ttcaactaaa tgttctcttt ttctgcttct tgcttttatt gattcttttg ccattttttc
    21481 gaattatttt ttagttagaa ccggttctga atggtagtcc gaattcttta agtaactgca
    21541 ttgcttcatt atcagtttta gctgtagtta cgaatgtgat atccattcca cgaatcttac
    21601 ttactttgtc aatattgatt tcagggaaaa tgatatgctc ggtaatgcca agtgtgtaat
    21661 ttcctttacc atcaaatcct ttgttattga ttcctctgaa gtcgcgaata cgtggcagcg
    21721 atactgacac caaacgatca aggaattcat acatctgctc atcacgcaaa gtcactctta
    21781 ctccaatagg aacacctttt cttactttaa agttagaaat atctttctta gcataagttg
    21841 ctacggcttt ctgtcctgca atagctgtca tttctgctac acaagcatca acaattttct
    21901 tatcactaac tgcatcgcca ataccctggc taagacaaat ctttgataat ttaggtacct
    21961 gcattatact cttatactga aaatgatttt tcagtgcaga gatgatttct tttttatact
    22021 tgccttttag gcgcggtgtg taactcatta ttttattacc tcccctgatt tttttgcgta
    22081 acgtactaat ttaccttctt ctgttttacg acctacacgt gttggctttc ctgacttagg
    22141 atcaataagc ataagatttg aaacattaac cggagcttct ttctttacga taccgccttg
    22201 tgtattttgt gcattcggac gtgtatgttt tgaaacgata tttacattct caacacttgc
    22261 cctgtttgtt tcaggaaata cttcaagcac acggccttgt ttgccttttg actcaccggc
    22321 aataacctta acgatgtcgc ctttttttat ctttagtttt gacatgattc tgattttata
    22381 aattaaatta cttcaggtgc caatgaaacg attttcataa actgtttttc gcgcagttca
    22441 cgagctacag gtccgaaaat acgtgtacct ctgagttcat ctgcctgatt gagtaacacc
    22501 actgcattat catcgaaacg gatataagaa ccatcattac gtcttacttc cttacttact
    22561 ctaacaacca cagctttgct tacagcacct tttttaatat ttcctgcagg taaagcattc
    22621 tttacggtta ccacaatttt atctcccaac gaagcatatc ttttgcgtgt accgcccaac
    22681 acacggatac acaaaacttc ttttgctccg ctgttgtctg ctactgttaa tcttgattct
    22741 gactgtatca tcttttattt cttttctaca ttctgccttt gagtagggcg agaaatttta
    22801 attattttgc tttttcaaga atttcaacta atctccaacg cttcgatttg ctgataggtc
    22861 tggtttccat aattaaaaca cggtcgccac taccacatgc attagtttca tcatgtacgt
    22921 gaaacttttt agtcttatta ataaactttc cgtacatagg atgtttaaac ttcttttctt
    22981 cagccacaac aacagtcttt tgcattttaa tgctggtaac cacacctatt tttgttttgc
    23041 gtaatgctct ttcagtactc attgaataat ttattttgat gtttgtgaca ttcttttgtt
    23101 taactcagtt ttcagccttg caataagttt gcgagtgctg cggattttca tcggattatc
    23161 cattggagaa acggcatgtt gcattttatg cttctgtaaa ttatccactt cttccttaat
    23221 gcgtgatttt acttcttctg ttgttaaatc tttaactatt gactgtttca tcgtttttca
    23281 aattttaatg ttaggcagct gaataatctc tgcgaataac aaacttggtt attaccggta
    23341 atttttgtgc tgccaaacgc attgcttctt tggctgtttc tacagacaca ccatctgctt
    23401 caaaaagcat agttcctggt tttacttggg caatccaata ttccggtgct cctttacctt
    23461 tacccatacg aacctctgca ggtttacgtg taacaggctt gtcagggaaa atacgtatcc
    23521 atgtttgtcc ttcacgtttc ataaaacggg taagggcaac acgggctgct tcaatctgac
    23581 gtgaagtaag ttgagcattc tccagtgatt taattccaaa tgatccgaag gcaatctgcg
    23641 atccgcgggt agcattacca cggttgcgca tcttctgctg ctttctgaat tttgtctttt
    23701 tcggttgtaa cattgctaat taaccttaat tcttttttaa ataattattt cttctctcct
    23761 cttggacgtc cacctcttct gccgccacgg tcattaccgc cacgttctct ctcctgtctt
    23821 ctgtcaccac cgcctgcatt ctgctttggt gttgagttca ttccaacatt tggagaaagg
    23881 tctctcttac caaaaacttc gcctttacaa atccaaactt ttacaccgat ttttccataa
    23941 gatgtttgtg catctgcaat ggcaaaatca atatcagcac ggaaagtatg caatggaata
    24001 cggccatctt tgtactgctc tgtacgagcc atctcagcac caccgatacg gccggcacac
    24061 ataattttta ttccttctgc tcccatacgc atgctggaag caattgccat cttaattgca
    24121 cgtctgtatg aaatacgtgc ttcaatttgt ttggcaacac catcagctac taactgtgca
    24181 tcaagttcag gacgtttaat ttcaaagatg tttatctgca cttctttctt ggtaatcttc
    24241 ttaatttctt ctttaagctt atctacctct gatccacctt taccgataac aatacccgga
    24301 cgtgcagtgt gaatggttgc agtgataagt tttagtgtgc gctcaataat aatacgggca
    24361 actccacctt ttgccaaacg tgcatgcaga tagttgcgga ttttttcatc ctcaactaaa
    24421 cggtcagaat agttctttcc accataccag tttgagtccc atccacggat gattcccagt
    24481 ctatttccta tcgggtgtgc tttttgtccc atttatgctt ctgtattttc agttacttgt
    24541 tttgtgtttt ctactttttt attgacagtg tcaactaata aggtaacgtg gtttgaacgc
    24601 ttacgaattc tgtagcctct accttgtggt gcagggcgta atcttttaag ctgacgggca
    24661 ctgtccacat aaactttaga tacaactaat ggctgatctt cgatgcgaac accttcgttt
    24721 tttgcctgcc agttattgat tgctgataac aacagtttat acatgttgcc tgcagcttct
    24781 tttttactga atttaagcaa gcctaatgca agatcaactt tcttacctct gatcatgtca
    24841 gctaccagtc tcatttttct tggagaggta ggacaattat tcaacctggc aatgtaggtt
    24901 gatttcattt cttctttgcg ggcatctgcc gctattttct ttcttgctcc cattatttct
    24961 tatctttatt acctgcatgt cctctgaaag tacgtgttgg tgcaaattcg cctaatttgt
    25021 gcccaaccat attttctgtt acatatacag ggataaactt attaccattg tgaactgcaa
    25081 atgtatgtcc aacaaaatct ggtgatatca aagagcgtct gctccatgtt ttgattaccg
    25141 tcttcttttt accctcactc attgacgcaa cttttccttc cagtttagga tcaatgaagg
    25201 ggcctttttt tagtgaacga ctcatttatt ttttattagc ttgaagcgta ttattaatta
    25261 tggagaatta tttctttctt ctttctatga tatgtttatt gctttgattt ttcagctttc
    25321 gggttttctt tcctttagct aacaaacctt tgcgcgaacg cggatggcca ccggatgcac
    25381 gaccttcgcc accacccatc gggtgatcaa cagggttcat ggccacacca cgaactcttg
    25441 gtcttctgcc tttccaacgg ttacgtcctg cttttccggc actttcttta ttatgatcag
    25501 aatttgaaac tgaaccgata gttgcaacac atgttgaaag caccattctt gtttcacctg
    25561 atggcatttt taagattgca tactttccgt cacgagctgt taattgagca taagaaccgg
    25621 cacttcttgc cattttacct ccctgtccgg ggcgcaattc aatgttatga acaattgtac
    25681 ctaatggcat gtctttcaac atcatagcat tgcccacctc aggtgttgct ttatctccag
    25741 aaagaatagt ttgaccaact gtcagtcctg ttggagcaag aatatatctt ttctcaccat
    25801 cagcataatg aactaatgca atacgtgctg tacggttagg atcgtactct atagttgcta
    25861 ctttaccggg aacaccgtcc ttattacgtt taaagtcaat aattctatat aacttacgat
    25921 gacctccacc gatatagcgc atggtcattt tacctgaatt atttcttcca cctttgcgat
    25981 taatacgctc tgtcaaagcc ttttcaggtt cgttggttgt tatgttagaa tgatctggcg
    26041 ctattaaaaa gcgctgacct ggtgtaatcg gattaaattt cttgattgcc attgttcaat
    26101 ttttatatgc ttgcgtaaaa atcaattgtt tcgcccttag ctacagtaat gtaagccttc
    26161 ttttgaccgc cctttttacc ttgcagaata cctgcttttg taaaacgggt ttttgatttt
    26221 acagcattat taactgtatt tacgctcaca acgttgacat tataaagctt ctcaatagca
    26281 gctttgattt caattttgtt tgcgttttta tctacgataa acgcatattg cccgagtttc
    26341 tcggacttct gcgtcatttt ttcagtaata actggcttct ttaagatgct catttgttta
    26401 atattttttc aattaccggc aaagcgcctt cttccaacac caaattttgc gcattaagaa
    26461 tatcgtaagt gtttatctct gaggcactta atactttact gcgttgtaaa tttcgggagg
    26521 acaaaaataa atttttattg gtatctccgg taactactaa aactttttta ttagtaagtt
    26581 ccaattgctt caaaatgttt accacttcac tggtttttgg agcttccatt gcaacattct
    26641 caacgatggt aatgttgttg tcttttgcct tatagctaag agctgaaata cgggcaagac
    26701 gtttaacttt cttattcagt ttgaatgaat aatcgcgtgg ctgaggaccg aaaacacgac
    26761 caccgccaac aaaaagtggc gatttcatac ttcctgcacg tgcacctccg gttccttttt
    26821 ggcgtttcaa cttcttagtt gtaccggaaa cttcgttacg ttgctttgac ttatgtgtac
    26881 cctgacgtgc atttgccatg atgcttttta catcaagcca tatagcatgg tcatttggtt
    26941 ttgcttcaaa gatttcgctg ttaagcgtta cttttcttcc agtagcctga ccgttttttt
    27001 gtttaatttc taattccatt tagcttaaaa ttctacttta atatatgaac ctttagcacc
    27061 tggtacagcg ccttttatga ttaaaagatt atgctcaggc attactttta aaatctgaag
    27121 attctgaatc ttcacctgct catcgcccat gtgacctgtc aatctttttc ctggaagtac
    27181 tcgtgatgga aaagatgatg ctcccataga acccggtgca cgcagacggt tatgctgacc
    27241 gtgcgtagca cctcctacac cggcaaagtt gtggcgtttt ataacaccct gaaaaccttt
    27301 accttttgag gttcctataa catcacaaaa ttcgccttcg ggacagatat caactttgat
    27361 aacatcgcca agtttcacct ctgtagtaaa gcctttaaac tctactactt tgcgcttaaa
    27421 tgagctgttt gatttcttaa aatgaccttg aagcgccttt gtagtatgct tttctttttt
    27481 atcatcaaaa gctaactgaa cggcatcata gccatcagta gctactgatt taacctgggt
    27541 tacaacacat ggacctgctt caattactgt gcaggcaatg tttttaccct cagcattaaa
    27601 catgctggtc attcctattt tctttcctat tattccggac attgttcttt actttaaggg
    27661 tttaactaca cttttatttc aacctcaaca ccgctgggta attctaattt cataagcgca
    27721 tctacagttt tagaggttga gctgtatata tccaataagc gcttatatga gcaaagctga
    27781 aactgctctc tagccttttt atttacgtgt ggcgagcgaa gtactgtgta aatcttcttc
    27841 tcagttggaa gaggaatggg tccgctgatc actgctcccg tagtcttaac ggtttttacg
    27901 attttctccg ctgacttgtc aacgagattg taatcgtaag atttgagctt tattcggatt
    27961 cgctgattca tttatttatt atttaatttc tttttaaaac ctacaattat gctttttcta
    28021 atttaccttt tactttggca attacttctt cggcaatggc acgaggacac tcagaatagt
    28081 gcgaaaactc catgcttgat gaagcacgtc ctgaagtaat ggtacgaagt gttgttacat
    28141 atccaaacat ttcagaaagt ggcacttttg ctttaattac ctgtgctcct gcacgtgtgt
    28201 ccattccttc catttgaccg cgtcttctgt taaggtcgcc cataacatca cccatgtatt
    28261 cgtcaggagt aacaacttca actttcatga ttggctcaag caaaatcggt tttgcctttg
    28321 cacaagcttc tttataagca agtttagcag caatctcgaa tgacaatgca tcggaatcaa
    28381 ctgcgtggaa agaaccatct gtcagaataa ctttaaggct gcttaaagga taaccggcaa
    28441 gtacaccatt aaccatagct gctttaaaac ctttttcgat tgaagggata aattctcttg
    28501 ggatatttcc accactgatt tgatttacaa attgcaggct tgatttgctt gcaataggat
    28561 caaaatcttc gtcaacaggg ctaagggtaa actgaatatc ggcaaattta ccacgaccac
    28621 cggtttgctt tttgtaaacc tcgcgatgtg aaacgctgcc tgtaattgct tctttataag
    28681 acacctcagg tgcaccctga ttacattcta ctttaaactc tctcttcaga cggtcaacaa
    28741 taatttcgag gtgaagttca cccataccgc tgatgatagt ctggcctgtt tcttcgtcag
    28801 tttttacacg gaaagttgga tcttcttcgg ctaacttatt cagtgcagtg cctaatttct
    28861 cagtatcaac ctgagttttt ggctcaatgg ctaatccaat aacggcttca ggaaactgca
    28921 ttgcttccag caccattggt gcattttctg cacaaagcgt atcaccagtt ttaatatctt
    28981 taaatccaac agcagcacca atatcaccag cctcgataaa aggaatctgg ttttgtttat
    29041 tggcatgcat ctggaaaata cgagaaatac gttctttatt acctgagcgt gtatttaata
    29101 cataagaacc ggactctaag cgaccagagt atgcacggaa atatgccaag cgacctacga
    29161 aaggatcggt agcaatctta aatgcaagag ctgtgaaagg ctcttttaca tcgggcttac
    29221 ggcttacagg ctcaccattg tcagggtttg ttcctgtaat attatcttta tccatcggac
    29281 ttggtaacaa ttccattacc aggtcaagca ttgtttgaac tcctttattc ttaaaagatg
    29341 atccacaaac cattggaaca actttacctg caatacatga ttttcttaat gcatcaagaa
    29401 tttcgcgctc ggtaatgctg tttgggtcat caaaatactt ctccatcagt ttatcgtcaa
    29461 attcagcgat agattcaaga agtttttctc ttaagctttt tgcttcttct aatatttctg
    29521 caggaatagg cacttcagta aatgtcatac cctgatcgtg ctcattccat acaatacctt
    29581 taaagttgat taagtcaacc acacctttaa agtgctcttc tgcaccaatt ggcaattgta
    29641 aag
//
LOCUS       DAOOOI010000017        29600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_278_length_29600_cov_39.460416, whole genome shotgun sequence.
ACCESSION   DAOOOI010000017 DAOOOI010000000
VERSION     DAOOOI010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 29600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29600
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_278_length_29600_cov_39.460416"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..>452
                     /locus_tag="PKX59_04070"
     CDS             24..>452
                     /locus_tag="PKX59_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90076.1"
                     /translation="MNKDSVWIEVIALAGQRSNLVSALLSSPYGMTDTINNGNSRIVT
                     GKYPIKNLAKLDSLPISSMINYCRPLYAPSVLSIGDRSKGDSAMRADIARNGFNLSGN
                     GIKVGLISDSYNTLKKANFDITNGELPGTGNPNGDLTNVIV"
     assembly_gap    453..490
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            518..>2384
                     /locus_tag="PKX59_04075"
     CDS             518..>2384
                     /locus_tag="PKX59_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOZ90077.1"
                     /translation="MAQIVYDVAPKASLHFRTGFNDANDFALGIKQLQQVGCKVIVDD
                     VTYITEPFFTDGVVAQAVDAAAAAGVSYFSAAGNFGNKSYESTFNPAPTPAGLSGTAH
                     NFAGGDILQKISFNEQGDYTIVLQWEDGIYSAGSITGTQNDLDIYLTDDAGKILVGYN
                     RKNTGQDPLEVLPISVPTASAVANIMIVRASGTGNVKFKYVVFRGDITFIEHGTLSST
                     IVGQSNANGAIAVAAMRYDKANLIEDFSSLGGTTVNGSVRNKPDVTAPDGVNTTINFG
                     TVFNDGDNIPNFFGTSAAAPHAAASAALIMEARTKFYSQAVTASDMKTLLKSTAKDIG
                     AAGFDFLSGSGLVQTDAAIRTFASPEAIIDTLIVPAGITPGTQAFSVTITGRYMNAQS
                     KVFLNGVQVPTAYVSSNQVNATIPLFNGNPPVTVYNPPTVPNMNDGGFSNELRFNSLK
                     KVVITVDNKTKKFGEMLPVFTSTVTVNGQTLAASGLSLAALKLSNLVYTTSATNMSDA
                     VTLPYYIHVENGSPLNAALPADALLMETYSYSYIDGNLNIERLPLTINSRDTTLVYGQ
                     KIDGQSFEYNYVFDNSKIAPADRAAFLNGVKTEHETAITNAVALVDERIVNGATGR"
     assembly_gap    2385..2427
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2428..4516
                     /locus_tag="PKX59_04080"
     CDS             <2428..4516
                     /locus_tag="PKX59_04080"
                     /inference="COORDINATES: protein motif:HMM:NF037085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HOZ90078.1"
                     /translation="LLNLSLAASSGAITNGRILSGQGGNSSLIVDLAPQSVFDFIDNV
                     DPLQLISGFAGENPRSLLDGDVITNRATGRVVTNGATGRVVTNRATGRVVTNSTPGTN
                     SNKKLAFLIDSIDVFDTITHEIESINSIHLITGTTVGTWRMTPAAFISDNFDVSYGIG
                     ALTITKAPLTIAADNKNKFYGSPNPPFTLTYSGFVNGDDESSVIPPVVSSPALTTSPA
                     GSYLIKVNGGTSSNYTLTKIPGTLTIKKVVLIATADNKSKVYGTANPPLTISYTGFVN
                     GEGVDSIASLPFAATNATVLSGVGNYAIALTGGSDNNYSFTKVNGTLSITKANLVAKA
                     DNKSKIYGSSNPAFTISYSGFVNGDDATSVSAPTASSSALKLSSVGNYPINLSGGSSS
                     NYSFTYQSGTLTINPAALIVSADNKTIAAGASLPTFTYSTSGFVSNGANTVASGPQYA
                     VSPVYSGAVGTYTISPSALVLSQPNNYSISYVAGTLTVSTSTTSSKKIEVELECVDTL
                     INDPSGFKYIARFEYENDNCYSVTIPIGTDNSLVGTYSGTQPSVFLPGENYFNVKFNG
                     SLLTWTVKSYEGTTKKTSTASATKSSRSCYCSSSRLAFVSESMEEVESTTFSLYPNPS
                     SDKIYVSSSFEFTSETSISVLDIVGREQFAQRVVKKDGNKLEMDIHSLTKGIYFVKIT
                     NGLEQFTLRFVKE"
     gene            4621..6696
                     /locus_tag="PKX59_04085"
     CDS             4621..6696
                     /locus_tag="PKX59_04085"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ90079.1"
                     /translation="MKKLKLAFFIFSIFLYPLAQAQSNSAELIANLKNSKDDTSKVTT
                     LLEISKSYFGISSGKSISYAELAKSLSKKINYKKGIALSYKCIGIANFNEGSYIEALQ
                     NYKQSLDVFASINDSIGVANMYSNMGNVYYYKGEDFKALDLYFKSLKIAELLGDKLRI
                     ASANANIGAVYIRKRSTYYKAIEFNLNALKIAEQINDKNILGTAAVNLGELYLDQGNT
                     ELALSYLQKSLKATEGTEIYASSLIAVGKVYSKKREYEMAIQYFKKAYDFAKSIDAKL
                     DITAALIEMGKVYEKTKEYKKALVQYNEAEKLASKLEAVELLKSSYEGLANSFGTIAD
                     FRNAFKYQKLLTSVNDTLYNIESTKKQNNLQFNYDLEKKQSEINLLQKEKELTTKEIK
                     NKQTIQFALLAFLAAVSGLLVFVYRFYIIKKKSNVQLEQKNKKIESQKSEITKSIEYA
                     KKIQDAMLPEEDLIAEVLFNSFLIYKPKDIVSGDFYVCINENKKIFLAVADCTGHGVP
                     GALMSMIGSNILNKLITDKGLTEPTEILEQLNSELIVSLKQNKNEGNDGMDIALCVID
                     FGARNLKFAGAYRPLWMIRNHELVEIKGSKFPIGGHQIHDERFFKTHSIPIYKTDRFY
                     IFTDGFADQFGGAYGKKLTTKKLKEYVLSTHTENIKNQGTSLSHFLEIWKGKNEQLDD
                     VCFIGFEVS"
     gene            6808..7935
                     /locus_tag="PKX59_04090"
     CDS             6808..7935
                     /locus_tag="PKX59_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOZ90080.1"
                     /translation="MKIAVNTRLFLKDKMEGMGWFGYESLKRIVQNHPEHRFIFIFDR
                     PYSPEFVFANNVTPVVLFPPTRHPFLWFVWLECMLPFVLRKHKADLFLSPDGMISLLS
                     NTKSLAVIHDLNFIYYKKDMPFWVEKYYNFFFPRFAKKSVRLATVSEYSKKDIVLNYG
                     IDADKIDVVYNGANLLYRPISEPEKTATKKEWSEGNDYFLFVGAMHPRKNIVNLFKAF
                     DAFKAKSKSVVKLVIVGNKMYWRADIEACFEAMQFKKDVVFTGRLSPEKLKNVLGAAM
                     ALTYVPYFEGFGIPIVEAFYCDVPVITSTITSMPEIAGDAALLVDPFSVTEIAEAMGK
                     IEGDADLRFALIEKGKIRRQEFTWDKTADKLWNCIEKALAT"
     gene            7940..8611
                     /locus_tag="PKX59_04095"
     CDS             7940..8611
                     /locus_tag="PKX59_04095"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOZ90081.1"
                     /translation="MLQKFLNSYGIEAGCDEAGRGCLAGPVVAAAVILPRKMNKALKA
                     LDDSKKLNEKQRMLLRPLIEKHAKAFAIGIVSNKEIDSINILNASFLAMHRAIEQLTL
                     EPDLLLIDGNRFNRYKNVKHECIIQGDGKFMSIAAASILAKTYRDELMEKLHAEFPVY
                     DWKKNKAYPTRFHRSMIEKNGISPYHRLSFTLLATQLELSFKSSLSKKIKKKQGKPNK
                     PKLHA"
     gene            8808..11333
                     /locus_tag="PKX59_04100"
     CDS             8808..11333
                     /locus_tag="PKX59_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90082.1"
                     /translation="MISYPFFGALHRDIHFIDGLFSQGSDALKQVADKPLFVSIHPDA
                     SNQFEFLYLINLPNTLTKKQVNDFILEAVSKEAKYNLRIYEGVALNELKLSENKALYY
                     FFNKGIFACTFSKFLMEDAIRQLNSGLSLSADAAFDKVWNAAGKKVEATLFINYQYAA
                     ELLSHVVDAKEAGFLKSLAGFANWTCLDLKSKKDAYQLTGFTFSKDSSNNYLSVFKNQ
                     EAQEMDIVSQFPAASAYFQWHGLSDVNRYFKNYDTYLNNLTNHYSRQEQLLNLNKKYG
                     VNAQNFFKQWIGNQFAYVVYEPGTENIMNSCYAVFKTNGTPSVHSTLLEFSSKVNTKQ
                     NAADTLNEIFRGYPIQHIAAENLVPLVFGETFEKLKNTYFVEMDKVVVVGNSAGAIKK
                     WINSVLAKQTLKEDLDYQSFAENISSQSSIYIYSALARSSRLFQNSIKKDVLNWNDSK
                     TETLKNFHATALQVSAKPSMFYSSIYASYKPCYKKPPASIWEIELDTTVHQRPYVLLD
                     PADNSTKLLVQDDAHKIYLIDNSGKIMWKRELKEKIGSEVQTLNIGKKGEMQILFSTQ
                     TKIYLLDDEGDNVGKFPVVLKYLATNGVQAVDYDENGSYRFLIAEENKRIYNFTQKGE
                     AVNGWEAVVNTDKIVSAIRSFRAKGLDYLVVVDQSGNVNLLDRKGKSVVKFKQKLKNL
                     YHGQYTLAVDETLNSSSVLGCDSSGKVTRLYFSGKISQVEVVKTRAPFYFYADASGSK
                     FVYVFQNKLEFYGGNFVKKSETKLNLSQLFSPAFFTTNNGDLMVSLAEEGKKTMLISE
                     DGLVVSEIECAATTAPMAARLFGDASWYTVLGAGKKVIAFAAY"
     gene            complement(11378..11764)
                     /locus_tag="PKX59_04105"
     CDS             complement(11378..11764)
                     /locus_tag="PKX59_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOZ90083.1"
                     /translation="MAFILDTHAFLWFVSGDKKLPENVKTKIMDINEPCFLSVASLWE
                     ITIKKQIGKLILDISLEELFDYADRNQIEIIQITYEHLLTLEQLPSHHNDPFDRLIIS
                     QALAENLTLITRDKGLKKYKIKQQWT"
     gene            complement(11764..11970)
                     /locus_tag="PKX59_04110"
     CDS             complement(11764..11970)
                     /locus_tag="PKX59_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HOZ90084.1"
                     /translation="MTNTSLKIEINSLPKSLRDEVADFVAFLKTKSVIKSSTLKQRQY
                     GFAKGKIQLSKDFDEPLDMFKEHM"
     gene            12505..14142
                     /locus_tag="PKX59_04115"
     CDS             12505..14142
                     /locus_tag="PKX59_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90085.1"
                     /translation="MKKYLFPKNQFFLLLLSLGLFLQSCVKEEYDMRDIEEGTWNPDI
                     AIPLVYTNFSINDIVLRTDKNGNIATDADNFCTLIYNGTLFSINAEDFVVLPNQSNAT
                     SFTVSALEQNAFNALPVNSSFTISRSQSISFQPGSTNAQIDSILFASGLINFQIDKTF
                     TAGTTVQIGIPAAKKNGLGYFDTFGVGLGTTTRSFNLAGYSLNLKDSSASHNKFKIYY
                     AITLTKTPGSSLPAGSNINFTEDIVNQKFQTVFGNVGMQSNLTPNNDTVAISIFRHNN
                     TPIGIGTFHFADPLLKLYLTNSFGLPVSISNIDLKWYNPGFGQGNIAFTSAPLQVNAP
                     IVVGGAEVSDTLKLNNSNSDFNAAMDNRPKYIIYNVSSLTNPNTMPPQVNFVDAGSKL
                     KANVEVVLPMFGNAKDFTFEDTVKFKFEKVDNIESILFKAAAVNDFPMGISMQVYFTD
                     SLYNKIDSLFSDVNVLPSATVSFLPSGLQTSVANQKITDVFFNQARALALKNAKFIII
                     RAISATFDDGTKNVKIYSDRRLSVKLGARAKMNIKIK"
     gene            14251..15393
                     /locus_tag="PKX59_04120"
                     /pseudo
     CDS             14251..15393
                     /locus_tag="PKX59_04120"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
     gene            15562..15964
                     /locus_tag="PKX59_04125"
                     /pseudo
     CDS             15562..15964
                     /locus_tag="PKX59_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009744597.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
     assembly_gap    15965..16064
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16065..16877
                     /locus_tag="PKX59_04130"
     CDS             <16065..16877
                     /locus_tag="PKX59_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ90086.1"
                     /translation="GCPDKDGDKIPDKDDDCADVIGLAKYRGCPDADGDGIKDSEDRC
                     PQKPGPIDNRGCPEDILYVIDGNGKVLSFATRGKDGVFRFETLPPDGDFKFRLEGEGT
                     ETIDKLNILVAGVSTRAMRMKDGFFKFEKIVADISKLTEEKVIDVPIKLMKEEQEILK
                     KAFDNLEFNTGKDIIRFESYASLDDLGKLMVKKSEWRIKLSGHTDNVGNAKANLTLSQ
                     KRAQAVKNFLVDRGIKPERILVEYFGGSKPVADNKTEAGKQKNRRVEMLVIE"
     gene            complement(17227..17994)
                     /locus_tag="PKX59_04135"
     CDS             complement(17227..17994)
                     /locus_tag="PKX59_04135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOZ90087.1"
                     /translation="MIKSNENLKTTGLRLALFFAIAYIVSNIFRFNVFQLNDALEKNT
                     TWFYLILEGLLEGSGIFIAGFVGLHLLRKQKASEISFFGSSKLKSTAMMLVPTVLLIV
                     LGVQNKYGINPHLFGFIAAFGTLLYCMMEEYGWRGYLQSELQFLQPVFRFFLIGCIWY
                     AWHLSFLTDTNVLNNLFFLGALIFGSWGIGKVAELTNSIAACACFHFLVNVFMFNHFF
                     NNAFSGNSKTILLFVSVLLWVLILIKWKKDTRQKFME"
     gene            complement(18134..18529)
                     /locus_tag="PKX59_04140"
     CDS             complement(18134..18529)
                     /locus_tag="PKX59_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HOZ90088.1"
                     /translation="MFTLDQIKEAHSKVKSGADFPAYIQEIKQLGVRSYETFVNDGHA
                     NFYGENAFRISSLAKYPALGVSDRSNAEQFILDLKAHQQGRTDFLTFCKDCANSGIEK
                     WEVDLSKMTCTYFDKAGNEMLVELVPKAK"
     gene            complement(18685..19938)
                     /locus_tag="PKX59_04145"
     CDS             complement(18685..19938)
                     /locus_tag="PKX59_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOZ90089.1"
                     /translation="MGKLTHHEFIILLISMAAMLILSRVLAEFGRKLKFPIVMGEIVA
                     GILLGPTILGIVFPSIFSEYFPSTGSVSIALDGIAKISVVMMLFVTGMEVQLPIVLKQ
                     GKAAIYTSVMGMVIPFASGFFVAFYFPFLFGITDEHFRLLFALFLGTALSISALPVIS
                     RILIDMNMFKTKIGTIIIASAMFNDLIGWLIFSFILGMLNTTGGEAQNVWLTLAYILG
                     FGLFMLTIGKMIIDKSLPWMQKKLSWPGGILSISLGICFLCAAYTESIGLHAILGAFI
                     AGIAFGDSVHLNEKAREIIHQFVTNIFAPFFFVSIGLKVNFITNFDLQLVVVILVLAY
                     IGKVVGASLGAYLGGFSKNESLAVGFGMNARGAMEIILGTLALNAGLINARIFVALVV
                     MALITSLTSGPLIHLFVSKEDAAKK"
     gene            complement(19945..20820)
                     /locus_tag="PKX59_04150"
     CDS             complement(19945..20820)
                     /locus_tag="PKX59_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOZ90090.1"
                     /translation="MDYSKLKKRPAFPFETIAVAVSFSPRLEAVLCEAKRISNLFGAQ
                     LVLLHVGDKTLEKEELLDAQIKKAGIDKNKSRLIWMDGEPIDTILKLCKLNIVDLLIL
                     GAIEKENLFRFYVGSIARTISRRAKCSVLLLTQPSNHPKRFKKIIVNGVDNPKTVHTV
                     KTVLYISKHEKLSEFTIVQEDDLPVLSMTIAESSTAPEATKIKKEMMQEENLKLQKIV
                     DALDKENINVNVKTVHGKPGYAISQYAKQKDADLLVFNSPDTHLGIFDRIFTHDIEYA
                     LANLPCNLLIVHSRV"
     gene            20949..21440
                     /locus_tag="PKX59_04155"
     CDS             20949..21440
                     /locus_tag="PKX59_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90091.1"
                     /translation="MKKQLVIALCLFLNLTQLGMGQDTRDPAQLLGRVNVKLLQGSQR
                     FGWFSASYSNYTCNASAIAQLTKQGSEISIFAFVGTWNAATQELLGPLYKVIDEAKIN
                     RNSVLLYFLDRDKKSPQGFEGTYMLSDVPTFIVFKNNQEIGRISGTVANSIEGDLADI
                     LLH"
     gene            21452..22540
                     /locus_tag="PKX59_04160"
     CDS             21452..22540
                     /locus_tag="PKX59_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOZ90092.1"
                     /translation="MRYPFYFKLASVLVCLVFGTIILIYAKDFLLPLLISILISFLLY
                     PLCKKMEKKKIPPILAISFSLLLVLFIFMFSLFLISSQLSSMLSDMKNLNQIMAAKLN
                     GLHDYITSNYNISNQTLNSWIVNAKSSLLGHSGEFLSGTFSTTTEILTYFGLVPVYVF
                     CFLLYRKSFRDFAFSMLHTQKHAKLNELLQDIQKLSQNYLIGLFTVIAIIGTLNTVGL
                     WIIGVDYALFFGFFASLLTIIPYIGIFIGSLLPIAFSLLTMDSVWAPIGIIIVFTLVQ
                     FLESNFITPKVVGSSVSINPFAAIVALLIGGELWGAAGMILSIPVTAILKVVFDSRTS
                     TKAIGYFLGSELTDTRDNPADTLTKTEA"
     gene            22590..23384
                     /locus_tag="PKX59_04165"
     CDS             22590..23384
                     /locus_tag="PKX59_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOZ90093.1"
                     /translation="MQKICVFIDFTDGCKIALKQASVLAQKNNATICLLHIVDAADEI
                     EKTKVHLLKFAKSTLGHSVLMEAYAGLGNLMDGAPAQLRKINPDLVIIGTHGIKGIKQ
                     KFLGADILKLVKVIEYPCIVVQENTTVKETGFAKILFPVGPHHDFLVKIKQTAKFAKT
                     FDCLVVIYEIAKEGFDLGGMVGKNSNLAKAHFVENNVSYISVTEEMTVFSAGNSRQTL
                     NYADKNSFDLISMMATISQNEILFGSTDKENLLVNSFGIPILCCNE"
     gene            complement(23734..24807)
                     /gene="holA"
                     /locus_tag="PKX59_04170"
     CDS             complement(23734..24807)
                     /gene="holA"
                     /locus_tag="PKX59_04170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOZ90094.1"
                     /translation="MAKKSESVSFEKIISDLKAKVYKPVYFLTGEEPYFIDAITDYIA
                     NHVLDESEREFNQSILYGKDIDTMRIISEAKRFPMMADKQVLIIKEAQEIKDLEKTTK
                     VTVGNKKKEEEINLLEEYVKNPQPTTILVICYKYKKLDGRKSLGKSLDKYSVLYTSAK
                     LYDNALPGWIEKLAKSQHLSIAPQASSLMAEYLGSDLSRIANEIEKLKINLRGRKEIQ
                     IDDVQNFIGISKDYNVFELQKAIGKRDVVKSNRIIGYFAANPKENPFVFVMANFYSYF
                     SKLLLYHGSPDKSEFGLARAIGVNSFFVKDYELAARIYTPEKLRRIIGFLREYDLKSK
                     GVESGDVGTGELMKELLFKILHC"
     gene            complement(24820..25593)
                     /locus_tag="PKX59_04175"
     CDS             complement(24820..25593)
                     /locus_tag="PKX59_04175"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOZ90095.1"
                     /translation="MKSKKDIVDNWLPRYTGTKLEDFGEYILLTNFNNYVKMFAEWHN
                     VEINGADKPMPNATFENITIINFGMGSANAATVMDLLSAISPKAALFLGKCGGLKKKN
                     ELGDLILPIAAIRGEGTSNDYFPSEVPALPAFMLQKAVSTTIRNHNKDYWSGTVYTTN
                     RRVWEHDADFKDYLRKLRCMGIDMETATIFITGFYNQIPTGALLLVSDQPMISTGVKT
                     AESDQKVTQNFVDEHLKIGIDSLLELINNGLTVKHLRFE"
     gene            25665..26132
                     /locus_tag="PKX59_04180"
     CDS             25665..26132
                     /locus_tag="PKX59_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOZ90096.1"
                     /translation="MLPTLNLPSFAVKLKNQSQRTQIFDRIRKKFVSLTPEEWVRQHF
                     LNYLIEHKKYPESRIAVESSLIYNELLRRPDIVYFDVFRKAQLIVECKAPEIKITQST
                     FDQIARYNIALKVRYLVITNGIQHFCCSMDYEKESYAFQKEIPDFSDFETSLQ"
     gene            complement(26119..27015)
                     /gene="dapA"
                     /locus_tag="PKX59_04185"
     CDS             complement(26119..27015)
                     /gene="dapA"
                     /locus_tag="PKX59_04185"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOZ90097.1"
                     /translation="MNSKFKGTGVAIVTPFKKDFSVDEPALKKLVNHLIDGKVEYLVI
                     LGTTGESVTLSKKEKAQVTACVVKETAGRIPLVLGLGGNHTAELLEQLSSTDFTGIDA
                     LLSVSPAYNKPNQEGIYQHFKAIAAASPLPIILYNVPSRTGSNMSAETTLRLAAEFPK
                     FIGIKEASGNLEQCMRIAKSKPKDFLLISGDDNLTIPMISIGAKGVISVIANAFPFET
                     SEAVRHALKDEFDKACGHYFSLMEITDLLFADGSPGGVKAVLKMMDICTDTVRLPLMN
                     VSNSVEEKLKALVDKKRAVIAG"
     gene            complement(27078..27623)
                     /locus_tag="PKX59_04190"
     CDS             complement(27078..27623)
                     /locus_tag="PKX59_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90098.1"
                     /translation="MGIVKNTKKRFGEYFLKKESEGLYRNKEAMNFSKAKNIGILFDA
                     TVEEDFDLVKKYIKFLKDSKKRVRSFGYYNTKEIPAMQYAKLEVDFFTKKQVNWHLKP
                     SDPLITNFTEEEFDILINFTVHQCLPLVYIAALSKAKFKIGKHFPKYEMYYDLLLEVE
                     EGKSLKYFMRNVDVYLNMINA"
     gene            complement(27692..>29600)
                     /gene="ligA"
                     /locus_tag="PKX59_04195"
     CDS             complement(27692..>29600)
                     /gene="ligA"
                     /locus_tag="PKX59_04195"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOZ90099.1"
                     /translation="DYEFDMLLEELVGLEKEFPEFLNPNSPSQRVGGTITKEFKTVQH
                     KYPMLSLGNTYSLEELNDFDIRIRKIVTEEFEYVCELKYDGVAIGLTYIHGKLVQAVT
                     RGDGVQGDDVTANVRTIKSIPLRLNAENLPEEFEVRGEIFMPRKSFDEINKEREEIDE
                     APLANPRNAASGTLKMQDSSNVARRNLDCYLYFLNGQNLPFSTHYESLEGLKSWGFKV
                     SPFTRLCKNSDDVAQFIAHWNTERNKLGFDIDGIVLKVNNYRQQQILGFTSKTPRWAI
                     SYKFKAEQVSTQLLSIVYQVGRTGAITPVANLQPVPLAGTLVKRASLHNADIIEKLDI
                     RVGDTVFVEKGGEIIPKIIAVDFSKRLHDSVPTEYAQSCPECGATLIRQAGEANHYCP
                     NELGCPPQIKGKIEHFVGRRAMNIDSLGAETIEQLFNAKLLNTLADIYDLQESQLLPL
                     ERMAQKSVSNLLDGILASTKVPFERVLFALGIRHVGETMAKKLAFNFKSLDNLMENLD
                     QLEQVDEIGPRIAQSVKAFFADPRNIEIINRLKAKGLQFELSQEEALNFSEKLKGLSF
                     VVSGVFSKFNRDDLKKVIEQNGGKNIGSISSKTSYVLAGENMGPAKLEKAEKLGVKII
                     TEDDFIKMLEE"
BASE COUNT         9323 a         5292 c         5656 g         9148 t
ORIGIN      
        1 atacaacacc ctttattttt aggttgaata aggatagtgt ttggatagaa gttattgcat
       61 tggccgggca acgttcgaat ttggtttctg cattgttatc ttctccttat gggatgactg
      121 atacgataaa caatggtaat tcacgtatcg taaccggaaa gtacccaatt aaaaatcttg
      181 caaaactgga tagcttgcca atttcttcga tgataaatta ttgccgtcca ttgtatgcac
      241 cttctgtttt atctattggt gacagaagta aaggagatag cgcaatgcgc gcagacattg
      301 ccaggaatgg cttcaatttg agtggaaatg ggattaaggt tggattaatc tctgacagtt
      361 acaacacact taaaaaagca aattttgata ttaccaatgg agaattgccc ggaacaggaa
      421 atccgaatgg tgatttaaca aatgtgatag tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn tttaagagta ttgatgaagg aagagcaatg gcacaaattg tatatgatgt
      541 tgccccaaag gcttctttgc attttcgcac aggatttaat gatgcgaatg attttgccct
      601 tggtattaag caattgcaac aagttggttg taaggtaatt gtagatgatg tgacctatat
      661 tacagaacca tttttcacag atggagttgt tgcacaagca gtagatgcag cagcagcagc
      721 cggtgtatcc tatttttcag cagccggaaa ttttggaaac aaatcctatg aaagtacatt
      781 taaccccgct cctactcctg ccggattatc gggcactgca cataattttg ccggtgggga
      841 tattttacag aaaataagtt ttaacgaaca aggtgattat actattgtac ttcagtggga
      901 agatggtatt tactctgcag gatccattac cggaacacag aacgatttag atatttattt
      961 aacggatgat gcaggaaaaa ttttagtggg ttacaaccgt aaaaatacag gtcaagatcc
     1021 attagaagta ttgcccattt cggtaccaac agcgagtgcg gttgcaaata ttatgattgt
     1081 aagggcatcc ggaacaggta atgtaaaatt taaatatgta gtatttagag gagatataac
     1141 atttatcgaa cacggtacac tatcttcaac tattgtaggc caatcgaatg ccaatggagc
     1201 aattgcagta gcagcgatgc gttatgacaa agccaattta attgaggatt tttcatcgct
     1261 tggtggaact acagtaaatg gaagtgtacg aaacaaacca gacgtgactg cacctgatgg
     1321 tgtaaatacc accatcaatt ttggaactgt atttaatgat ggtgacaata ttccaaactt
     1381 ctttggaacc tcggcagcag ctccacatgc ggctgcttcg gctgccttaa ttatggaagc
     1441 tagaactaaa ttttacagcc aagcggtaac agcatctgat atgaaaacgc tacttaaaag
     1501 tacggccaaa gatattggtg ctgccggatt tgatttttta agtggttcag gattggttca
     1561 aaccgatgca gccattcgaa cctttgcaag ccctgaagca attattgata ccttgattgt
     1621 tcctgccgga attacacccg gaactcaagc cttttcggta acgattacag gacgatacat
     1681 gaatgcacag tcaaaagtat ttttaaatgg agtgcaagta ccaactgctt atgtcagttc
     1741 aaatcaggta aatgcaacca tccctctatt taatggtaat ccaccggtta ctgtttacaa
     1801 tccaccaacc gttccaaata tgaatgatgg cggattctcg aatgaattgc gttttaattc
     1861 tttgaaaaaa gttgtaatta cagtcgacaa taagaccaaa aaatttgggg aaatgttgcc
     1921 ggtctttact tccacagtta ctgtaaacgg acaaaccttg gcggctagcg gtttaagttt
     1981 agcggctctg aaacttagca atttagtgta tacaacttcc gcaaccaata tgagcgatgc
     2041 agtaaccctt ccttattata tccatgttga aaatggcagt ccactcaatg cagctttgcc
     2101 ggctgatgct ttattgatgg aaacctattc ttattcctat attgatggaa atttaaacat
     2161 tgaaagattg cctttgacaa taaattcaag agataccacc ttggtatatg gacaaaaaat
     2221 agatgggcaa agttttgagt acaactatgt ttttgacaat tctaaaatag caccggcaga
     2281 tagagcagca tttttgaacg gtgtaaaaac tgaacacgaa actgccatta cgaatgcagt
     2341 tgcgcttgtt gacgagcgaa ttgtaaacgg tgccacagga agagnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnttt actcaatttg agtttagctg ccagtagtgg
     2461 agcaattact aatggaagaa tcctaagtgg tcagggtgga aactcttcac tgatagtaga
     2521 tttagccccc caatcggtat ttgactttat tgacaatgtg gatcctttgc aattaatcag
     2581 tggctttgct ggtgaaaatc caagaagttt actagatgga gatgtaatta cgaatcgtgc
     2641 aaccggaaga gtggtaacga atggagcaac cggaagagtg gtaacgaacc gagcaaccgg
     2701 aagagtggtt accaacagca ctccgggcac aaacagcaat aaaaaattag cctttttgat
     2761 tgactcgatt gatgtatttg atacaattac ccacgagata gaaagtatta attccattca
     2821 cttaatcacc ggaactacag ttggcacttg gagaatgaca ccggcagcct tcatctctga
     2881 caattttgat gtgtcttatg gaattggtgc cttaacaatt accaaagcgc cactaaccat
     2941 tgctgctgac aataaaaata aattttatgg aagccctaat ccacctttta cattaacgta
     3001 ttctggtttt gtgaatgggg acgatgaaag ttcagttatt ccaccggtgg taagtagtcc
     3061 ggcactcacc acttcgccgg caggctctta cttaattaag gtgaatggcg ggacttccag
     3121 taactatacc ttaacgaaga tacccggaac attgacaatt aaaaaagttg ttcttatagc
     3181 tacagccgac aacaaaagca aggtatatgg aactgccaat cctcctttaa cgataagcta
     3241 tacaggcttt gtaaacggag aaggagttga ttctattgct agtttgccat ttgctgcaac
     3301 aaatgctact gttttgtcgg gtgttgggaa ttatgcgatt gccttaaccg gaggttctga
     3361 caacaactat tcattcacca aagtaaatgg tacattaagt atcacaaagg caaatttagt
     3421 agcaaaagct gacaataaat ctaaaattta tggaagttcg aacccagctt tcacaataag
     3481 ctattcagga tttgttaacg gagatgatgc aacttctgtt tcagcgccaa cagccagtag
     3541 cagtgcttta aaattatcat cggttggtaa ttatccaatt aacttaagcg gtggctcttc
     3601 gagcaattat agttttacgt atcaaagcgg aaccttaaca attaatcctg cagcattgat
     3661 tgtgagtgcc gataataaaa caattgcagc tggggcttca cttcctacat ttacgtattc
     3721 cacttcagga ttcgtgagta atggagccaa tactgttgca tcgggaccac aatatgcagt
     3781 aagtccggtt tacagtggag cagttggaac ctacacgata agcccatccg cgctggtgtt
     3841 aagtcagcca aacaactatt caataagtta tgtggcaggt actttgacag tttccacctc
     3901 caccacaagc agcaaaaaaa ttgaagtgga gctagaatgt gtggatacat taatcaatga
     3961 cccatccggc tttaaatata tcgctcgctt tgaatatgag aacgacaatt gttattcggt
     4021 aaccattcca attggaactg acaattcact tgtaggaacc tatagtggta ctcagccgag
     4081 cgtattccta cccggtgaaa attattttaa tgtaaaattc aacggatcgt tattgacgtg
     4141 gacagttaaa agttatgagg gaacaacaaa gaaaacgtca acggcaagtg caacaaaaag
     4201 ttcaagaagc tgttattgct cttcttctcg attggcattt gtttccgaat cgatggaaga
     4261 agtagaatca acaactttct ctctttatcc caacccaagc agtgataaga tttatgtaag
     4321 ttcctcgttt gagtttactt cagagacctc tatttcggta ttagacattg ttggtcgtga
     4381 acaatttgca caacgtgttg taaagaaaga tggcaataaa ttagagatgg atattcattc
     4441 cttaacgaaa ggaatttatt ttgtaaaaat cacaaacgga ttagaacaat ttacactgcg
     4501 atttgtgaaa gagtaaaaag aaaatacata aaagaaagag ggggaaatat tctccctctt
     4561 ttttttgtca agtacaaaag atttgtaaat tgcacgtgca aataattact tatccaaagt
     4621 atgaagaaac taaagttggc cttctttatt ttttcgattt ttttataccc gcttgctcag
     4681 gctcaatcaa attcagctga attaatcgcc aatctaaaga acagtaagga tgacacttcc
     4741 aaagttacta ccctgcttga aattagtaaa agctattttg gcatttcctc cggtaaatca
     4801 atttcgtatg cggaattggc taaatcactt tcaaaaaaaa ttaattataa gaaaggcatt
     4861 gcactttcat acaaatgcat tggtatagct aattttaatg aaggctctta tatagaagca
     4921 cttcaaaact acaagcagtc actggatgtt tttgcttcga ttaatgatag cattggagtt
     4981 gccaatatgt atagcaatat gggcaatgtt tattattaca agggggaaga tttcaaagct
     5041 ttggatttgt actttaaatc tttaaagatt gcagaactac ttggtgacaa acttcgcata
     5101 gctagcgcta atgccaatat tggagcggta tatattcgca aacgatctac ttattacaag
     5161 gccatagaat tcaatttaaa tgctttaaaa attgcggagc aaattaatga caaaaacatt
     5221 cttggaaccg ctgcggtaaa tttgggcgaa ttgtatttag accaagggaa cacagaactg
     5281 gccttaagct atttacaaaa atcgttgaaa gctacagagg gaactgagat ttacgcttct
     5341 tcgcttattg ctgtagggaa agtgtattct aagaaacgtg aatatgaaat ggcgatacag
     5401 tattttaaaa aagcgtatga ctttgctaaa agtattgatg ccaaattgga tataacggca
     5461 gcattaattg aaatgggcaa agtgtatgaa aaaacgaaag aatataaaaa agccttggta
     5521 cagtataacg aagctgaaaa attagcgagc aaactggaag ctgttgagtt attaaaatca
     5581 tcgtatgaag gacttgcaaa ctcgtttgga acaattgctg attttagaaa tgcatttaaa
     5641 taccaaaaat tattgaccag tgtaaatgat accctgtata atattgaatc caccaaaaag
     5701 caaaacaatt tacagtttaa ctatgacctt gaaaaaaagc aaagtgaaat aaatttattg
     5761 caaaaagaga aggaacttac cactaaagaa attaagaaca agcaaaccat acagtttgct
     5821 ttgctcgcat ttttggctgc tgtaagtggt ttacttgttt ttgtgtatcg cttttacatc
     5881 atcaagaaaa agagcaatgt gcaactggag cagaagaaca aaaaaattga atctcaaaaa
     5941 tcagaaatca ctaaaagcat tgagtatgct aaaaaaattc aggacgccat gttgcctgaa
     6001 gaagatttaa ttgcagaagt tcttttcaat agttttttga tatataaacc aaaagacatt
     6061 gtaagtggtg acttttatgt gtgtataaac gaaaacaaaa aaatattttt ggcggtggct
     6121 gattgcaccg ggcatggagt tccgggagct ttgatgagta tgattggaag caatatatta
     6181 aacaaattaa ttaccgataa aggcttaaca gagccaacag aaattttaga gcaactcaac
     6241 agtgaattaa ttgtatcgtt gaagcaaaat aaaaacgaag gcaatgacgg tatggacata
     6301 gcgctttgtg taattgattt tggtgctcgt aatttgaaat ttgcaggagc ctatagaccg
     6361 ttatggatga ttcgaaacca tgaattggtt gaaataaaag gttctaaatt tcctataggt
     6421 ggtcaccaaa tacatgatga acgatttttt aaaacccaca gtataccgat ttacaaaacg
     6481 gatcgttttt acattttcac ggatggcttt gctgaccaat ttggcggtgc ttacggcaaa
     6541 aaattaacca ctaaaaagct aaaggaatat gtactttcaa cacacactga aaacataaaa
     6601 aatcaaggta cttcgttgag tcatttttta gaaatttgga aaggtaaaaa cgagcaattg
     6661 gatgatgttt gctttattgg ttttgaagtg agttaaatcc cattttgtat ctcaaattaa
     6721 actaacactg cgctgttaag tacttaaaaa aactcttctc ctttttttct tttgttggct
     6781 tacttttgct ttacaaatac tgtaaagttg aaaattgctg taaatacaag gcttttctta
     6841 aaagataaaa tggaaggcat gggttggttt ggatacgaat ccttaaaaag gatagtacaa
     6901 aatcatcctg agcatcgttt catttttatt tttgacaggc cctattcacc cgaatttgta
     6961 tttgcaaata atgtaactcc tgttgttctc ttccccccta cccgacatcc ttttttatgg
     7021 tttgtatggt tggagtgtat gctacctttt gttttaagaa agcacaaagc ggatttattt
     7081 ttatcgcccg atggcatgat aagcttgcta tcgaatacca agagtttggc tgtaattcat
     7141 gatttgaatt ttatctatta caaaaaggat atgccatttt gggtggagaa atattacaat
     7201 tttttctttc cgcgttttgc aaaaaaatca gttcggttgg caactgtttc agaatattca
     7261 aaaaaagaca ttgtgttgaa ttatggtatt gatgccgaca aaattgatgt ggtatacaat
     7321 ggtgcgaacc tgctgtaccg tccaatttcg gaaccggaaa aaacagccac aaaaaaagaa
     7381 tggagtgaag gaaatgatta ttttttgttt gtgggtgcca tgcatccacg aaaaaatatt
     7441 gtgaatttat ttaaagcatt tgatgcgttc aaagcaaaaa gtaagtcagt ggtaaaattg
     7501 gtaattgtgg gcaacaagat gtattggcgg gcagatattg aagcttgctt tgaggccatg
     7561 cagtttaaaa aggatgtggt gtttaccggt aggcttagtc cggaaaaact aaaaaatgtg
     7621 ctgggtgctg ccatggcact tacctatgtg ccctactttg aaggatttgg aatacccatt
     7681 gtagaagcat tttattgtga tgttcccgta attacctcca ctataacttc gatgcccgaa
     7741 attgccggtg atgcagcatt actggtcgac ccgttttcgg taaccgaaat tgccgaagcg
     7801 atgggaaaaa tagaaggaga tgctgatttg cgttttgcat taattgaaaa aggaaaaatt
     7861 cgcaggcaag aatttacctg ggacaaaaca gccgataaac tttggaactg cattgaaaaa
     7921 gcactcgcca cctaaaccca tgctacaaaa atttttaaac tcctatggaa ttgaagcagg
     7981 atgcgatgaa gccggtagag gctgtttggc aggaccagtg gttgctgccg cagtaatact
     8041 tccacgcaaa atgaataaag cattaaaggc attagacgat tcgaaaaagc tgaatgaaaa
     8101 acaacgcatg ctgctgcggc ctctcattga aaagcatgcg aaagcctttg ctattggaat
     8161 cgtatccaat aaagaaattg actccataaa tattttgaat gcttcgtttt tggccatgca
     8221 ccgtgcaatt gaacaattaa cgctagagcc ggatttatta ctaatagatg ggaatcgatt
     8281 taaccgttat aaaaacgtga agcatgaatg catcattcaa ggagatggaa aatttatgtc
     8341 gattgcagca gcatccatat tggcaaaaac atacagagat gaattgatgg aaaaattaca
     8401 tgccgagttt cctgtttacg attggaaaaa aaacaaggcc taccctactc gatttcatcg
     8461 ttctatgatt gaaaaaaacg gcatcagtcc ttatcaccgc ttaagcttta ccttattggc
     8521 tactcagctc gaactttctt ttaaaagttc cttgagtaaa aaaattaaaa aaaagcaagg
     8581 caaaccaaat aaaccaaaac tacatgctta aaaaaatact tgttacctta tttattgtcc
     8641 cggtaattgg cgctgctatt tatggttact ttcattttaa acaaataaaa acacccttaa
     8701 gtccggtaat acaagctata ccgagcaacg cggcttttat tttaaagagc aatacaccca
     8761 aagcctcttt gcaaaatttt tcacttcaac aaaacatctg gaaaagtttg atttcatatc
     8821 cattttttgg cgcattgcac cgcgatatac attttataga tggtttattt tcgcaaggca
     8881 gtgatgcgtt gaagcaggta gctgataaac ccttatttgt ttcgatacat ccggatgctt
     8941 cgaatcaatt tgagtttttg tatttgatta atttacccaa tacactcacg aaaaagcagg
     9001 taaatgattt catactagaa gcggtttcga aagaagcaaa atacaattta agaatctacg
     9061 aaggggtagc actcaacgag ctaaaattaa gtgagaacaa agccttgtat tatttcttta
     9121 acaaaggaat ttttgcttgc actttttcta aatttttaat ggaggatgct attcggcagc
     9181 tcaattcagg actttcactt tctgcggacg cagcatttga caaggtgtgg aatgcggccg
     9241 gaaaaaaggt ggaggcaact ctttttatca attaccaata tgcagccgag ctgcttagcc
     9301 atgtggtaga tgcaaaggaa gcaggctttt taaaatcact agcaggattt gccaactgga
     9361 cttgcctgga cttaaaatca aaaaaagatg cttatcaact caccggcttt actttttcga
     9421 aagacagcag caacaattat ttgtcggttt ttaaaaatca ggaagcacaa gaaatggaca
     9481 ttgtttcgca atttcctgct gcttctgctt attttcaatg gcatggattg agtgatgtaa
     9541 atcgctattt taagaactac gacacttatt tgaacaattt aacgaatcat tattcgagac
     9601 aggagcaact tttgaatttg aataagaaat acggagtaaa tgcccaaaat ttctttaaac
     9661 aatggattgg aaatcaattt gcgtatgtgg tatatgaacc gggaacagag aatattatga
     9721 attcctgcta tgctgttttt aaaaccaatg gcaccccatc agtacattcc acactgcttg
     9781 aatttagcag caaagtgaat acgaagcaaa atgcagccga cactttgaat gaaatttttc
     9841 ggggataccc aatacaacat attgcggcag aaaacttggt tcctttggtt tttggagaaa
     9901 catttgaaaa gttaaagaac acttattttg ttgaaatgga taaagttgtt gtagttggca
     9961 attcagctgg cgccattaaa aaatggataa atagtgtgct tgccaaacaa accttgaaag
    10021 aggatttaga ttaccaatcc tttgctgaaa atatttcttc tcaatcttcc atttatattt
    10081 attccgcgtt ggctcgttcc agtcggctat ttcaaaacag tattaaaaag gatgtattga
    10141 attggaatga ttcaaaaact gagaccttaa aaaactttca tgccactgct ttgcaagtga
    10201 gtgctaagcc aagtatgttt tacagttcta tttatgcttc ttataaaccc tgttataaaa
    10261 aaccgcctgc tagcatttgg gaaatagaat tagacacaac tgttcatcaa agaccctatg
    10321 tgttgttaga cccagccgac aacagcacaa aattacttgt gcaagacgat gcccataaaa
    10381 tttatttgat tgacaacagt ggaaaaataa tgtggaaaag ggaattgaaa gaaaaaattg
    10441 gcagtgaggt gcagacgttg aatataggta agaaaggaga aatgcaaatt ttgttttcaa
    10501 cgcaaacaaa aatttatttg ttggatgatg agggcgataa tgttggaaag tttccggttg
    10561 ttttaaagta tttggccacc aatggcgtgc aagctgtaga ttacgatgaa aatggcagct
    10621 atcgcttttt aattgcagag gagaataagc gcatttataa ttttactcaa aaaggggaag
    10681 ctgtaaatgg ctgggaagcg gttgtgaaca cggataaaat agtatccgca attcgtagtt
    10741 ttcgtgcaaa aggattggat tatttggtag tggtagacca gagtggaaat gtaaatttat
    10801 tggacaggaa aggaaaatcg gtggtgaagt ttaaacaaaa gctaaaaaat ttatatcatg
    10861 ggcagtatac acttgcggtg gatgaaacat tgaattcctc atcggtgctg ggatgtgact
    10921 ctagcggcaa agtgacgcgt ctttattttt cgggaaaaat tagccaagtg gaggttgtta
    10981 aaacccgtgc gccattttat ttttatgcag atgctagtgg aagtaaattt gtgtatgttt
    11041 ttcaaaacaa gctggaattt tatgggggca attttgtcaa gaaatctgaa acaaaattga
    11101 atttatctca actgttttca ccagcctttt ttacaaccaa caacggagat ttgatggttt
    11161 cgctcgcaga ggaaggcaaa aaaacgatgt tgatttcgga ggatgggttg gtggtttcgg
    11221 aaattgaatg cgctgccact actgcaccta tggctgcaag gttatttgga gatgcatctt
    11281 ggtatacggt gctgggtgcg ggaaaaaagg taattgcttt tgctgcttat taattattta
    11341 gaaaacatct tgcttgcaag ctattataag taggcggtta tgtccactgc tgcttaattt
    11401 tatacttttt aagtcctttg tcccttgtaa taagtgttag attttctgcc agtgcttgtg
    11461 aaataattaa tctgtcaaaa gggtcgttat gatgtgaagg taattgttca agtgtcaaaa
    11521 gatgttcgta tgtaatttga attatttcaa tctgatttct gtcagcgtag tcaaaaagtt
    11581 cttcaaggga tatgtcaagt atcagtttac caatttgttt tttaatagta atttcccata
    11641 gactagcaac acttaaaaag caaggttcat tgatgtccat aatttttgtt ttgacatttt
    11701 cgggcagttt tttgtcgcca gaaacaaacc aaagaaaagc atgggtgtca agtatgaaag
    11761 ccattacata tgctctttaa acatgtctaa tggctcatca aagtctttag aaagctgaat
    11821 ttttcctttt gcaaacccat attgtctttg cttaagtgta gaagatttaa tcacactttt
    11881 cgtcttgaga aaggcaacaa aatcagctac ttcgtctcgg agagactttg gaagtgaatt
    11941 aatttctatt ttcaaggatg tatttgtcat gctgtaaatt tataaaaatg tcaacaaatt
    12001 gcaaacttta ctggaaaatt gcacactcta ttccgctaac ttataactat ttactcccct
    12061 ataaacgctg ctttgcatta gtgattgagc gttttgtttg agctcctttg tgccttcaaa
    12121 ttttatgaag aagagttgca caggtccggc acaaagagcg agtagcgaaa gcacgacccc
    12181 gatgaaagct gctgtgcgag gcagctgtga atagccggca ggatttattg ggggaatgcc
    12241 caattaatga aagctataca agagttgctt ttgctacaga acttttaagt gttgaaaaaa
    12301 ttactttccg aaaattaatt tctgttttct gaaaaattga ttcgttgcat aaaatatagg
    12361 tattcaagga ttttcatgtc tgctatttgc ttcttctatt cctttcatgg aattattttc
    12421 taacatttta tcaaaaggcg attaggcgtg aaaaaaaata tttatcttgc gtattgcttt
    12481 tgacgaacta aaaagaagcg ccttatgaaa aaatatttat tcccaaaaaa tcaatttttc
    12541 cttctactcc ttagtcttgg tttattcctg caatcgtgtg tgaaagagga gtacgatatg
    12601 cgcgatattg aagaaggtac ctggaatccg gacattgcga taccgcttgt gtacaccaat
    12661 ttttcgataa acgacattgt gctgcgcacc gataagaatg ggaacattgc aaccgatgcc
    12721 gataattttt gtactctaat ttataacgga accttgtttt cgattaatgc ggaagacttt
    12781 gtagtgcttc ccaatcaatc caatgctact agttttacgg taagtgcttt ggagcaaaat
    12841 gcttttaatg ccttaccggt taattcctct tttactataa gccgctcaca aagcattagt
    12901 tttcaaccgg gaagtaccaa tgcacaaatt gattccattt tatttgccag tggattaatt
    12961 aattttcaaa ttgacaaaac atttactgca ggcacaacgg ttcaaattgg tattccggca
    13021 gctaaaaaaa atggattggg ttactttgat acctttggag ttggcttggg aacaaccact
    13081 cgcagcttta atttggcggg ctattcctta aatttaaagg attcatcggc aagccacaat
    13141 aagtttaaaa tttactacgc cattacccta accaaaacac ctggttcgag tttgccggca
    13201 ggcagtaata tcaattttac ggaggacatt gtgaatcaaa aatttcagac cgtttttgga
    13261 aatgtgggta tgcaaagcaa tttaactccg aataacgaca cagtagcaat cagtattttt
    13321 cgtcacaaca atacacccat tggaattgga acatttcatt ttgccgaccc cttgttgaaa
    13381 ttgtatttga cgaattcatt tgggctaccg gtgagtatca gcaacattga tttgaaatgg
    13441 tataatccgg gatttggcca aggaaacatt gcctttacct ccgcgcccct gcaagtaaat
    13501 gcacccattg ttgttggagg ggcagaagtg agtgacacct taaaattaaa taattcgaac
    13561 agtgatttta atgcggctat ggacaatcgc ccgaagtaca ttatttacaa tgtttcatcg
    13621 ctcacgaatc cgaacacgat gccgcctcag gtaaattttg tggatgccgg aagcaagtta
    13681 aaagccaatg tagaggtagt gttgccgatg tttggaaatg caaaagattt tacttttgaa
    13741 gatacggtta aatttaaatt tgaaaaggtt gacaacattg aatccatctt gtttaaagca
    13801 gccgctgtga atgattttcc gatgggtatt tcgatgcaag tatattttac cgattcactt
    13861 tacaacaaaa ttgattcctt gtttagtgat gtaaatgtgt tgccatcggc cactgtaagc
    13921 tttttaccga gtggattaca aacctctgtt gccaaccaaa aaataacgga tgtattcttt
    13981 aatcaagcgc gggcattggc attgaagaat gctaaattta ttattattcg ggccatttcg
    14041 gctacgtttg atgatggcac aaagaatgta aaaatataca gtgacaggcg actgagtgtg
    14101 aagttgggtg cgcgagcaaa aatgaatatc aaaataaaat aaaaacctcc aaaaaagata
    14161 ttcaagatga aaactctact cagcaatcaa tcctacatta aaaaaacaat tgcattattt
    14221 gtagttgtag tactttcttt tcatttttca atggatgtga aagggcagca aaatttaacc
    14281 ttatataatt taaaagcgat tccccaatcc aattatgtga atccgggaat tttgccagcg
    14341 agtaggttta atattggatt gccggttatc tcctcgcttt atttaaataa gagcaacagt
    14401 ggttttaagt tgaatgactt ggtaaccgat ggagccggaa gttctaccaa attggatttt
    14461 gaaaatttgg tcaatcagct ggccaccaac aattattttg cgctcgcctt gcaaacggat
    14521 ttattgagcc ttaactttaa atcgaaagaa aactatttta gtttcaacag cagttacaaa
    14581 gtaaattggc gtgtgcgcta tccgaaagat tttttaaact ttatttggaa agggaatggg
    14641 gcgtctttgg gcgaggaatt gaaatttaat ttaggcttgg atgctagtac ttatttggaa
    14701 tatggcttag gttatagccg tgaaattggc gacaaattat cgcttggctt acgtgtaaaa
    14761 tatttaagtg gtattgcaaa tgtatcgacc gaaaagagcg acatcacttt gtataccaat
    14821 ccgataacgt atgatattac agcaacctct gacattgttg taaatacttc gggaatagtg
    14881 gataatccct ttagcggaat gggtgtagtg gatgtggcta ccaagctgaa gaaccgtgga
    14941 tatggtattg atattggtgg aagttataaa gcaacggagc ggttaacagt gaatgcgagt
    15001 gtgctcgatt tgggttctat taaatggaaa tttgctccta ccaattacgt gagtggaaat
    15061 ccaaatgcaa gtttcaccta ccaagggatt aatttaaata cgtttgtaaa cgatagcacc
    15121 tccattgaca aggccgtgga attggtattt gattcactca atgcaacatt tgctttggat
    15181 actgttcacc gcagttataa aacccgattg ggagcacaaa tttatgccgg agcgcaatat
    15241 gaattaaaca aagaaagcaa tgtttccttg ttgcttcagg gtcatttttt tgataaaaaa
    15301 ttgcatcctg ctttggctgt ttcctataac tatacaattg acaattactt gatgggctcc
    15361 atcaattatt ccatgtttaa cagaagttac atgaacatag ggcttgggct tgcgcttaat
    15421 ttgggaccgc ttcaagtgta tggtgtgagc gacaatgtgc tgggactttt tatttacaac
    15481 aaatataaat ttgataccgg caatggtaaa aaaacggaga taacctatcc cggaaatacc
    15541 aaaaatttga atttacgtgt gggtataaat ttaacttttg ggcgtaaacc gttggataac
    15601 gacaaggatg gtatactgaa taaaaaagat gcttgtcctg agatttttgg cttagaagaa
    15661 tttgcgggtt gtccggataa ggatggcgat aaaattccgg ataaagatga taaatgcccg
    15721 gatgttgccg gattgaaaga attttttgga tgccccgata aagatggaga caaaataatt
    15781 gatggggaag ataattgtcc ggatgttccc ggattggctg aatttaaagg ttgcccggat
    15841 aaggatgggg ataaaattcc ggataaggat gacgagtgtg tagatattgc aggtttagca
    15901 gaatttaaag gttgtccgga taaggatggc gataaaattc cagacaaaga tgatgattgt
    15961 gcggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggttgt ccggataagg
    16081 atggcgataa aattccagac aaagatgatg attgtgcgga tgtgattgga cttgctaaat
    16141 atagaggttg cccggatgca gatggagacg gtataaaaga cagtgaagac cgctgcccac
    16201 aaaagccagg accgatagat aacagaggtt gtcctgagga tattttgtac gtgatagatg
    16261 gcaatgggaa ggtattgtcg tttgctacac gaggtaagga tggtgtattc cgatttgaga
    16321 ccttgccgcc ggatggagat tttaaattta gattagaagg agaaggaacg gaaactatag
    16381 ataagttaaa catattggtt gcaggtgttt ctacgcgtgc catgcgcatg aaggatggct
    16441 tttttaaatt tgaaaagata gttgcagaca tttcaaaact caccgaagaa aaagtaattg
    16501 atgtgccaat taaattaatg aaagaagagc aggaaatttt aaagaaagca tttgataatt
    16561 tggagttcaa taccggtaaa gatattattc gatttgagtc atatgcctcg cttgatgatt
    16621 tgggtaaatt gatggtgaag aaatcggaat ggagaataaa gttgagtgga cacaccgaca
    16681 atgttggaaa tgcaaaagcc aatttgacgc tttcccaaaa acgtgcacaa gcggtaaaaa
    16741 actttttagt tgaccgcggc ataaaacccg aaagaattct tgtggagtat tttgggggta
    16801 gtaagcctgt ggcggataac aagacagaag ctggcaaaca gaagaacaga cgtgtggaaa
    16861 tgttggtgat tgaatagcta agctgcttgc tgaatttaat atcgttgtct gctcctctat
    16921 gtggataaaa gagaagggag caagcaccgg agcaatgccc tcaagggaaa caaccttttg
    16981 gcgtagcaag aatgagctat acaaaagtaa ggtttctaaa aaggagcata atacattttt
    17041 caaagtggtg aagggaaatt aatttacaga catgccgata gaagccggat ttgtaatgaa
    17101 agaataggaa gggaaatgga gtggttataa tgccagcagt atacttaaaa ctgaagtgct
    17161 tggattcaga gcattgttct atcgattaaa aaccacatga agcaacgctg ggcgggtgct
    17221 gagtatttat tccataaatt tctgccgggt atcttttttc cattttataa gaataagtac
    17281 ccaaagcaaa acagaaacaa acaaaagaat ggtttttgaa tttccactga atgcattgtt
    17341 aaaaaaatgg ttgaacataa aaacattgac cagaaaatgg aaacaagcac aagctgcgat
    17401 ggaattggtg agttcggcta cttttccaat gccccaactt ccaaaaataa gtgctcccaa
    17461 aaaaaataaa ttgttaagca catttgtatc cgtgaggaag cttaaatgcc aagcatacca
    17521 tatgcatcca ataagaaaaa acctaaaaac aggttgaaga aattgcaatt ctgattgtaa
    17581 atatcctctc caaccatatt cttccatcat gcaataaagc agggttccaa aagctgcaat
    17641 gaacccaaaa agatgaggat tgattccata tttattctgc actccaagta caattaataa
    17701 cactgtaggt accaacatca ttgcagtgct ttttagttta gagcttccaa aaaaagaaat
    17761 ttcgcttgcc ttttgtttgc gaagaaggtg caatccaaca aatcctgcaa taaatattcc
    17821 acttccttct aagagacctt ccagaattaa ataaaaccaa gtagtatttt tctccagtgc
    17881 atcgttcagt tgaaaaacat taaaccgaaa aatatttgaa acgatataag caattgcaaa
    17941 aaacaaagcc aatctaagtc ctgtcgtttt taaattctca tttgatttaa tcattgtagc
    18001 agataatttt tgagtgcgta ataaattatg gacttattat ttttgaattc tgaaagggtt
    18061 tggtaaaaaa ttccactcgc ttaggggttc aattttgatt gtagaaatga aattcgcttt
    18121 ttcttctcca ctcctacttt gcctttggca caagttcaac taacatttca tttcctgctt
    18181 tgtcaaaata agtacaggtc attttactga ggtccacttc ccatttttca attcctgaat
    18241 ttgcgcagtc tttgcaaaaa gtaaggaagt ctgttcttcc ttgttgatgc gctttgaggt
    18301 ccaatatgaa ttgttcggca tttgatctat ctgatacacc aagggccgga tactttgcta
    18361 atgaggaaat ccgaaaagcg ttttcaccat aaaaattagc atgtccatcg ttcacgaatg
    18421 tttcatagct gcgcacaccc aattgtttta tttcctggat ataggcggga aagtcagcac
    18481 ctgacttcac tttgctgtgc gcttccttga tttgatcgag tgtaaacatg aatggatatt
    18541 tattgggtta attaaaagtt gaaatattgt tgaggccttt ccctgaaact tttttggcaa
    18601 ttttcataaa tcagaagtgg aaaagtaaat aatgatttta tttccgaaaa gtgatatgca
    18661 actaaataca gaaaactagt ccattcattt tttggcagca tcctctttgc tcacaaacaa
    18721 atgaatgagc ggtccactgg tgaggcttgt aattaatgcc atcactacca aggctacaaa
    18781 aatgcgggcg ttgattaatc cggcattcaa ggcaagcgta cccaaaataa tttccattgc
    18841 ccctctggca ttcattccga acccaacggc aagagattca ttttttgaga agccccccaa
    18901 atatgcgccc agactcgcgc ctacaacttt tccaatatag gccaagacta aaatcaccac
    18961 cacaagctgt aagtcgaaat tggtaataaa atttacctta agaccgatgc ttacaaaaaa
    19021 gaagggtgca aaaatattgg ttacaaattg gtgtataatt tctcgcgcct tttcatttaa
    19081 atgaaccgaa tcaccaaaag caattccggc aataaaagca ccgagtatgg catgcagtcc
    19141 aatgctttcg gtataggcag cgcacaaaaa acaaatgcct aaagagatag ataaaattcc
    19201 accgggccag gataattttt tttgcatcca aggcaaactc ttatcaataa tcattttacc
    19261 aatggttagc ataaacaatc caaatcccaa aatataggca agcgttaacc acacattttg
    19321 tgcttctcct ccagttgtat ttagcattcc taatatgaat gaaaatatga gccatccaat
    19381 taaatcatta aacatggcag aggcaatgat aatagttcca attttggttt taaacatatt
    19441 catatcaatc aaaattcttg atattacagg tagtgcagaa atacttaagg cggtacctaa
    19501 aaataaggca aagagcaacc gaaagtgctc atccgtaatt ccaaaaagaa aaggaaagta
    19561 aaaagcaaca aaaaagccac ttgcaaaagg tataaccatg cccataacac tggtataaat
    19621 tgcagctttt ccttgtttga gtacaatggg taattgcact tccattccag taacgaatag
    19681 catcatcacc accgaaattt ttgcaatacc atctaatgca atactaacac tacctgttga
    19741 tggaaaatac tcgctaaaaa tggaaggaaa tacaattccc agtatggtag gccctaataa
    19801 tatcccagcg acaatttcgc ccatcacaat ggggaacttc aatttacggc caaactctgc
    19861 aagtaccctg cttaaaatta gcatggcagc cattgaaata agtaaaataa taaattcgtg
    19921 atgtgtgagt ttgcccataa actactaaac gcgcgaatga actatgagta aattacaagg
    19981 aagattcgca agtgcatatt caatatcgtg agtaaaaatt cggtcaaaaa ttcctaaatg
    20041 ggtatccggc gaattaaaca cgagcaagtc ggcatctttt tgttttgcat actgactgat
    20101 ggcatagccc ggttttccat gcactgtttt tacgttcaca ttaatatttt ccttatcgag
    20161 tgcatccaca attttttgca gttttaagtt ttcctcttgc atcatttcct ttttaatttt
    20221 tgtggcttcg ggagcagtgc tactttctgc aatagtcatt gaaagcacag gcaaatcatc
    20281 ttcttgcaca atggtaaact ccgaaagttt ttcgtgtttg ctgatataca aaaccgtttt
    20341 aaccgtatgc accgttttgg gattgtccac cccgtttaca ataatttttt taaagcgttt
    20401 gggatgattg gaaggctgcg tgagaagcaa aaccgaacat ttggcgcgtc tgctgattgt
    20461 acgcgcaata gagcctacat aaaaacggaa taaattttcc ttttctattg ctcctagtat
    20521 caacaagtca acaatattga gtttgcacaa ctttaatatg gtatctatgg gctcaccatc
    20581 catccaaatc aatcgacttt tatttttatc gatgcccgcc ttttttattt gcgcatccaa
    20641 aagttcctct ttttctaatg ttttgtcacc cacatgcaag agcacaagtt gggcaccaaa
    20701 caagttggaa attcgctttg cttcgcacaa tacagcctcc aaacgtggcg aaaaggaaac
    20761 ggctactgca atggtttcga aaggaaaagc gggacgtttt ttaagttttg aataatccat
    20821 tttatataaa tttaggaaga tttcagatgg gtaaagatag gtaaaggcgg ctaggaaaaa
    20881 acaaaaatta gaagcttttt tattttgaaa gattacttaa ttttataaaa aattaaaaaa
    20941 tgtatgggat gaaaaagcaa cttgtcattg ccttgtgttt gtttcttaac ctgactcaat
    21001 tgggtatggg gcaagatacc agggatccgg ctcagttact gggaagagta aacgtaaaat
    21061 tgttacaagg tagtcagcga tttggttggt ttagtgcaag ctattcaaat tatacctgca
    21121 atgcatccgc cattgctcag cttacaaagc aggggtccga aatatctatt tttgcctttg
    21181 tgggtacttg gaacgctgct actcaggaat tgttgggtcc tttatacaaa gtaatagacg
    21241 aagcaaaaat caatcgaaat tcggtattgc tttatttcct cgacagggat aaaaaatcac
    21301 cacaaggttt tgaagggacg tatatgcttt cggatgtacc aacttttatt gtatttaaaa
    21361 ataaccaaga aattggaaga atatcgggaa cagttgccaa cagcattgaa ggcgacttag
    21421 cggatatttt acttcactaa ataatttgcc tgtgcgctat ccattttatt ttaaacttgc
    21481 ttctgtattg gtttgcctag tttttggaac catcatttta atatatgcca aagacttttt
    21541 attgcctttg ttgatttcca tattgatttc atttttgctt tatcccttgt gcaaaaaaat
    21601 ggaaaagaaa aaaattccgc ccattttagc catttccttt tcgctgcttt tggtgttgtt
    21661 tatatttatg ttttcgcttt ttttaatttc ttctcaattg tcgagtatgc tttcggacat
    21721 gaagaacctg aatcaaataa tggccgcaaa attgaatggc ttgcacgatt acataacttc
    21781 caattataat atttcaaacc aaactttaaa ttcttggatt gtgaatgcca aatcatcact
    21841 tttggggcac agtggagaat ttctttccgg aaccttttca accactaccg aaatattaac
    21901 ttattttgga ttggtgccgg tgtatgtatt ttgctttttg ttgtatcgaa aatcatttcg
    21961 ggattttgct ttttcgatgc tgcacacaca aaagcatgcg aagcttaacg aacttttgca
    22021 agacatacaa aagttgagtc aaaattacct catcggattg tttaccgtta ttgccatcat
    22081 aggaacctta aatacagttg ggctctggat tatcggtgta gattatgccc tcttttttgg
    22141 attttttgct tctttgctta ccattatccc gtacatcgga atatttattg gctctttgct
    22201 tcctattgca ttttcattgc ttaccatgga ttctgtttgg gcgcctatag gaataatcat
    22261 tgtgtttacc ttggttcagt ttctggagtc gaattttatt accccaaaag tagtcggttc
    22321 aagtgtaagc attaatcctt ttgcagccat tgtagctttg ctgattggcg gtgaattatg
    22381 gggagctgcg ggaatgattt tatccattcc ggttacggca atattaaagg tagtgttcga
    22441 cagccgaact tctaccaaag cgattggata ttttttaggc agcgaactca ctgatacgag
    22501 agataaccct gcagatacct taacaaaaac agaagcatga gtactgaatt tatttttttt
    22561 gtaatattac cctcctaaga aaaattacta tgcaaaagat ttgtgttttt attgatttca
    22621 ctgatggatg taaaatagct ttaaaacaag catccgtttt agcgcaaaaa aacaatgcaa
    22681 ctatttgttt gctgcacatt gttgatgctg cagatgaaat agaaaaaacg aaagttcatt
    22741 tgctcaaatt tgccaaatct actttaggtc attccgttct tatggaagcc tatgccggtt
    22801 tgggaaattt aatggacggt gctccggctc aattgcgcaa aatcaaccct gatttagtaa
    22861 taattggaac acatggaata aaaggcatta agcaaaaatt tttgggtgcc gatattttaa
    22921 aattggtaaa agtcattgaa tatccttgta ttgtggtgca agaaaataca accgtaaagg
    22981 aaaccggatt tgcaaaaatc ctcttccctg ttggccccca tcatgatttt ttagtgaaga
    23041 taaaacagac tgcaaaattt gccaaaacat ttgactgctt ggtagtgatt tatgaaattg
    23101 cgaaagaagg atttgattta ggcggtatgg ttggtaaaaa tagcaatctg gcaaaagcac
    23161 attttgtaga aaacaatgtg tcttacattt ctgttaccga agaaatgacg gtgttctccg
    23221 ctggaaattc acgccaaaca ctgaattatg cagataagaa ttcgtttgat ttaattagta
    23281 tgatggcaac catttctcaa aatgaaattt tatttggctc tacggataaa gaaaacttgt
    23341 tggtaaattc attcggaatt cctattcttt gctgcaacga atagtgtttg aacatcctag
    23401 ttagcttggt ttccatgtga gaattttttc tctttatcgc tactgaccaa aatttatctg
    23461 ttttttgatt ccgtgtttac ttgtagttat tttcaaaagc ataaaagggg ttacaagaat
    23521 tttgtttgga aatggctgta tttattcagg ctagtgtcta ttctttactg ctttgaattt
    23581 ttccaagtcc aaataaataa taccccaatt gtgtctatca aaatcttgaa tggttcttag
    23641 ctgcatatag ctttcatcca ctattgggct tgtttcaatg cctccattta cgaatataca
    23701 tccatattaa aacttcacga aagttaaact aaatcaacaa tgaagaattt taaaaagaag
    23761 ttctttcatg agttcacctg tacccacatc accgctttcc actccttttg attttaaatc
    23821 gtactctctt aaaaagccaa taattcttct taatttttcg ggtgtataaa ttcgagctgc
    23881 caattcatag tcttttacaa aaaaagaatt tacaccaatg gcacgagcga gtccaaattc
    23941 agatttatca ggtgaaccgt gatataagag cagcttactg aaataggaat aaaaatttgc
    24001 cattacaaaa acaaaggggt tttccttagg attggcagca aaataaccga tgatgcgatt
    24061 ggatttcact acgtctcttt ttccaattgc cttttgcaat tcaaacacat tgtaatcttt
    24121 gctgatgccg ataaaattct gcacatcgtc aatttgtatt tcctttcttc ctctcagatt
    24181 aattttaagc ttttcaattt catttgcaat gcggctcaaa tcacttccca aatattccgc
    24241 cattaaggaa gacgcttgag gagcgatgga taaatgctgg ctttttgcca atttttcaat
    24301 ccaacccgga agagcattgt cgtataattt ggccgaggtg tataacacac tgtacttatc
    24361 aagtgatttg ccaagtgatt ttcgtccatc cagctttttg tatttatagc atatcactaa
    24421 aatggtggtg ggttgaggat tcttcacata ctcttccagc aaatttattt cttcctcctt
    24481 ttttttattt ccaacggtta ccttagtagt tttctcgagg tctttgattt cttgtgcctc
    24541 cttaatgatt agcacttgct tgtcggccat cataggaaat cgcttggctt cactgatgat
    24601 gcgcatagtg tctatatcct tgccatagag gatggactga ttgaattctc tttcgctttc
    24661 atccaacaca tgattggcaa tataatcagt tattgcatca ataaaataag gctcctctcc
    24721 agtgagaaaa taaaccggtt tatatacttt tgcttttaaa tcgctgatta ttttttcaaa
    24781 agaaacagat tccgattttt ttgccatgct gaaatctgat tattcgaaac ggaggtgttt
    24841 cactgtaaga ccattattta ttaattcaag caaggaatca attcctattt tcaaatgttc
    24901 atccacaaaa ttttgggtaa ctttttgatc actttctgcc gtttttactc cggtagaaat
    24961 catgggttga tcactcacta aaagcaaagc accggtagga atttgattgt aaaaaccggt
    25021 aataaaaatg gtagccgttt ccatatcaat tcccatgcaa cgtaattttc gcaggtaatc
    25081 tttaaaatca gcatcgtgtt cccaaactct acggttagtg gtataaacag ttccactcca
    25141 gtaatcttta ttgtggttgc gaatcgtggt agacacagcc ttctgaagca taaaagcagg
    25201 taaggccggt acttcagatg gaaaataatc atttgatgtt ccttcacctc gaattgcagc
    25261 aatcggcaat atcaagtcac ccaattcatt tttctttttt aatcctccgc attttcctaa
    25321 aaacaatgct gctttgggag aaatggcact gagcaaatcc attaccgtag ccgcgttggc
    25381 actgcccatt ccaaaattaa taatggttat gttttcaaaa gttgcattgg gcatgggttt
    25441 atcggcccca ttaatttcaa cattgtgcca ttctgcaaac atctttacat agttgttgaa
    25501 attggtcaac aaaatatact caccaaaatc ttctaattta gttcctgtgt aacgcggcaa
    25561 ccaattgtct acaatatctt ttttactttt catgctttca tatttctact cattacttct
    25621 atacgcgaag caaagtttat atttgtttcg aaattttaac ttttatgctt cctacgctca
    25681 accttccttc cttcgctgta aagctaaaaa accaatctca aagaacgcaa atttttgaca
    25741 gaatcagaaa gaaatttgtt agtcttactc ccgaagaatg ggtaaggcaa cactttctaa
    25801 attatttaat agaacataaa aaatatccgg aatcacgcat agctgtagag tcctcgctta
    25861 tttacaatga attgttaaga aggccggaca tcgtttattt tgatgtattt aggaaagcac
    25921 aattgattgt agaatgcaaa gcacctgaaa taaaaatcac gcaatcaact tttgaccaaa
    25981 tagcgcgtta taacatcgct ttaaaggtgc gctatttagt aataacaaac ggcatacagc
    26041 atttttgctg cagcatggat tatgaaaagg aaagttatgc tttccaaaaa gaaattccag
    26101 atttttcaga tttcgaaact agcctgcaat aacagctctt ttcttatcca ccagtgcttt
    26161 caatttttct tctactgagt tgctcacatt cattaatgga agtcgaaccg tatccgtgca
    26221 aatgtccatc atttttaaaa ctgcttttac tccacctgga ctgccatctg caaaaagcaa
    26281 atcagtaatt tccatcaaag aaaagtaatg gccacaggct ttgtcaaact catcttttaa
    26341 agcatgtcga acagcctctg acgtttcaaa cggaaaggca ttggcgataa cagaaataac
    26401 cccttttgca ccaatcgata tcatcggaat cgttaaatta tcatccccgg atatcaacaa
    26461 aaaatctttg ggttttgatt ttgctatacg catgcattgc tccaaatttc cactggcttc
    26521 cttgatgcca ataaatttgg gaaactctgc tgccaatctc agggtagttt ctgcactcat
    26581 gttagaaccg gtgcggctag gaacattgta aagtataatt ggcaaaggcg aagcagctgc
    26641 aattgcttta aaatgctgat aaattccttc ctgattgggt ttattgtaag caggtgaaac
    26701 agaaagcagt gcatcaattc cggtaaaatc agttgacgaa agttgctcca atagttcagc
    26761 agtatggtta ccgcctagtc ctaataccaa tggaattcgt cctgcagttt ctttcacaac
    26821 acaagccgtt acttgcgcct tttccttttt gcttaaggtg acagattctc ctgtagtacc
    26881 caaaatgact aagtattcca ctttgccatc aatcaagtga ttcaccagct tttttaatgc
    26941 cggttcatcc acagaaaaat cctttttaaa aggagtcaca atagctactc cggtaccttt
    27001 aaattttgaa ttcatgtttt cttttaattt tcagtttata gcaacacaca gtattgcctt
    27061 gagtgtaatt ccttttttca ggcgtttatc atattcaaat acacatccac atttctcata
    27121 aaatatttga gacttttacc ttcttccact tctagcaaca aatcgtaata catttcatac
    27181 ttgggaaaat gcttgcctat tttaaatttg gctttactca atgcagcaat ataaaccaga
    27241 ggcaaacatt gatgaacagt gaaattaatt aaaatatcaa actcctcttc cgtaaaatta
    27301 gtaattaagg gatcgcttgg tttcaagtgc caattgactt gcttcttggt aaaaaaatca
    27361 acttctaact tggcatactg catggcggga atttctttgg tattgtaata cccaaaggag
    27421 cgtacccgtt ttttactgtc ttttaaaaat ttaatgtatt tctttaccag atcaaaatct
    27481 tcttccactg tagcatcaaa gagaattcct atattttttg ctttggaaaa attcatggct
    27541 tctttgtttc tgtatagacc ttccgattct tttttaagaa aatattcacc aaaacgtttt
    27601 tttgtgtttt ttactattcc caaggtttct aatgagtaat tgattaaatt tttttatttt
    27661 taattgaatg atttactaca aaatttgaaa gctactcttc caacatttta ataaaatcat
    27721 cttctgtgat tattttgact cctaactttt ctgccttctc taattttgcc ggacccatat
    27781 tttctcctgc caaaacataa ctggtttttg aagaaatgga acctatattc ttgccgccat
    27841 tttgttcaat tacctttttc aaatcatccc tattaaattt tgaaaatact ccggaaacaa
    27901 caaacgacaa ccccttcagc ttctcgctaa aatttaaagc ttcctcctga gagagttcaa
    27961 attgaagacc tttcgctttt aaacgattta tgatttcaat atttcttggg tctgcaaaaa
    28021 atgcttttac gctttgcgca atgcgtggtc caatttcatc cacttgctcc aactgatcca
    28081 aattctccat taggttatcg agcgacttaa aattaaacgc caactttttg gccattgttt
    28141 ctcccacatg ccttattcca agagcaaaca atacccgttc aaatggaact tttgtggatg
    28201 ctagaattcc atctaacaaa ttggaaacac ttttttgtgc catgcgctcc aatggaagca
    28261 attgtgattc ttgtaaatca taaatatcgg caagcgtatt cagaagtttg gcattgaata
    28321 attgttcgat tgtttcagca cccaaactat caatattcat tgctctgcgg ccaacaaaat
    28381 gttcaatctt acctttaatt tgtggcggac atcccaattc attgggacaa taatgattgg
    28441 cttcacctgc ttgtcgaatt aatgttgctc cacattcagg acacgactgt gcatactccg
    28501 ttggcacaga atcatgcaag cgtttggaaa aatcaactgc aataatttta ggaataattt
    28561 cacctccctt ttccacaaat acagtatctc ctacacgaat atcaagtttt tcaataatgt
    28621 cggcattgtg caaggaggct ctctttacaa gtgttcctgc taaaggtacc ggctgtaaat
    28681 tggcgacagg agtaattgca ccggtgcgtc caacttggta tacaatggac aaaagttggg
    28741 tggaaacttg ctctgcttta aatttatacg aaattgccca acgtggtgtt ttacttgtaa
    28801 aacccaatat ttgctgttgt cgataattgt ttacttttaa aacaattcca tctatatcaa
    28861 atcccaactt gtttctttcg gtgttccaat gcgcaataaa ctgagcaaca tcgtcactat
    28921 ttttgcacaa tcgggtaaac ggtgatactt taaatcccca actttttaat ccctctaaac
    28981 tttcataatg agttgaaaaa ggcaaatttt gaccgtttaa aaagtataaa tagcaatcta
    29041 aatttcttct tgccacgtta gatgaatcct gcatttttaa tgttcctgat gccgcatttc
    29101 gcggattagc tagtggtgct tcatcaattt cctcgcgttc tttattgatt tcgtcaaatg
    29161 atttccgagg cataaatatt tccccacgta cttcaaattc ttcgggcaaa ttttcagcat
    29221 tcaaacgtaa aggaatgctt ttaatggttc gaacatttgc agttacatca tcgccttgaa
    29281 ctccatctcc gcgtgttaca gcttgcacca atttaccatg gatataggtc aagccaattg
    29341 ccacaccatc gtattttaac tcgcatacat actcaaattc ctctgtcaca attttgcgaa
    29401 tacggatatc aaaatcattt aattcttcaa gagaatatgt atttccaagc gaaagcattg
    29461 gatatttatg ctggactgtt ttaaactcct tggttatggt accacccact ctttgggagg
    29521 gagaattggg atttaaaaat tccgggaact ccttttccag gccgaccaat tcctctaaca
    29581 acatatcaaa ttcataatcg
//
LOCUS       DAOOOI010000018        28850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_294_length_28850_cov_26.651606, whole genome shotgun sequence.
ACCESSION   DAOOOI010000018 DAOOOI010000000
VERSION     DAOOOI010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 28850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28850
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_294_length_28850_cov_26.651606"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PKX59_04200"
     CDS             <1..348
                     /locus_tag="PKX59_04200"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOZ90100.1"
                     /translation="YYVKAVYDTLNVYSTSNEVTLIQEPSVFTPNSFTPNGDGLNETW
                     SPVSSFIKNYNLKIFNRWGNLIFESNESNRQWDGSYQGQPAPLGIYFYVVTYIGFPDN
                     TKFNKRGSVTLLR"
     gene            418..3324
                     /gene="uvrA"
                     /locus_tag="PKX59_04205"
     CDS             418..3324
                     /gene="uvrA"
                     /locus_tag="PKX59_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOZ90101.1"
                     /translation="MQEIESLSPKKYIIIKGASVHNLKKVDVAISRNSFTVITGLSGS
                     GKSSLAFDTLYAEGQRRYVESLSSYARQFMGRLDKPEVEYIKGIAPAIAIEQKVNTRN
                     PRSTVGTSTEIYDYLKLLFARIGKTYSPVSGKLVKKNTVADVVDILKTQNQNKRFIIA
                     APLQFHPGRTLHEELNALMQKGFSRIVVDDKMLRIDEYAEQIEKTDAQPAVKTRGRKK
                     KVESIEPTINTNIFIVVSRIIIPENINEEYESSTADSVQTAFNEGSGRCTLIDITDET
                     LHVTNFSNYFEEDNIRFEEPSTHLFSFNNPYGACKNCEGFGSVIGIDENLVIPNKTLS
                     IFDGAVACWRGEKMNEWQQEFILKAYKFNFPVHRPYYELNETDIKLLWEGNKTIKGLN
                     DFFKYVESQSFKIQYRVLLSRYRGKTTCPECKGTRLRADANYVKVNSKSITDIVLMPV
                     NVSVEFFNNIILDSYEQKVAGRLLVEIKNRLQYLNDVGLGYLTLNRLSKTLSGGESQR
                     INLATSLGSSLVGSMYILDEPSIGLHPRDTQRLIYVLKSLQKAGNTLIVVEHDEEIMR
                     NADELIDIGPFAGTHGGKIVFQGNFEQLEHSTESVTAKYLTGKEFIHVPSKRRNWTNS
                     IEITGASENNLAHIDAKFPLGILTAVTGVSGSGKSTLVKKVLYPALKKVLGGYDDESG
                     KFEKLTGDFTTLNGVEIIDQDPIGKSSRSNPVTYVKAYDEIRALMADTPFARQKMMKP
                     SLFSFNVDGGRCEMCQGEGVVTIEMQFMADIQLKCETCQGKRFKNEILAITYREKNIF
                     DILSMTVDDAISFFSKETDERYILFTNKIIARLQPLSDVGLGYISLGQSSSTLSGGEA
                     QRIKLASYLGIGHATTKTLFIFDEPTTGLHFHDIRKLLDAFTALIRNGNSILVIEHNM
                     DVIKCADWVIDMGPGGGNEGGKIVFAGTPEALSNCNESYTGKFLSEKLIAQQEL"
     gene            complement(3327..4208)
                     /locus_tag="PKX59_04210"
     CDS             complement(3327..4208)
                     /locus_tag="PKX59_04210"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOZ90102.1"
                     /translation="MIINIVKSVASVVGLMVTKIPKTDYKEELYKSLFNNDSLAYKRF
                     YNVGAGPFNHKYWTNVDYVNDWYADNTNNTLKGINYDLFALEPLPIETDSAELVYTSH
                     TIEHVNNAAVQNLFNESFRVLKPGGRVRVVTQDIKLSYRAYKENDRHFFFWIEWFSKP
                     ENYKRVNLRQPLSQESLAQIFLEDFAAQASEIPLHGAKQRISDSELEKLFEEKSFEDA
                     LDYCTSLCDIEVQKKYTGNHINWFTEEKLIRMLKAAGFKSIYRSGYGQSYSPVMRDLN
                     FFDETLPGISLYVEAQK"
     gene            complement(4276..6984)
                     /locus_tag="PKX59_04215"
     CDS             complement(4276..6984)
                     /locus_tag="PKX59_04215"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90103.1"
                     /translation="MSKVFLTLVTVAVMSINLSSAQCPPLGAFSGMTGDFKACPGMSL
                     TYTLTPVTNAISYTWNLPPGATISGQNPYTTSSNVVTVDYGPLFSAPGSLCVTAYNGC
                     VNSAPYCKALTPAGAPSGASTISGSIFACPGVTETYSVTNQVNRVFDWTIPNNATLLT
                     GQGTNSITVQYNAGFNGGNICVRVGNGCAWSGFRCMTIYAGVPGTPTAITGPTEVCTG
                     TMTYSTGPSNGVPVTSYTWTVAPGVTLVGQGNDTVQITFPINYNYTDIYVKANNGCGS
                     SSNRSVRVRATPETPQPIIGLASGICNGTTPYTVPSSPTAVSYNWTLTGPGTILTGQG
                     TNNINIDYPNTFISGKICVTITNACATGSPRCLLTSKNIALSEQPQDFETCNSTQAMF
                     SVGALGMNLQYQWRKNGAPLSNGGKISGANSDTLYITGADSTDADQYDVIVSNNCGSS
                     ITSNTATLDIKQVPAKPGAISGVQATTCPGTSGIIYSVPLQMDATSYLWTYTDGVQIL
                     NGQGTDAVTLAFDSTINSGYSVFVYAVNECGQSLDSASSWTRYKVSTPTITGPARVCE
                     NLPGVNYMAPAVAGASSYNWTIPAGATLVSGQTTNAIVVDFGPTYTGGDVTVTASNIC
                     FTTPVKKLATVIDMPSVPTSLSGQVYGTCKTQLVYTAGNSNHATSYKWTLPPNSSIIL
                     GNFTNTVTVDFQNPGSGTYSLCVAGNNACRTGTYRCIQVRAIPERPASIVANPSVFCA
                     NQTGVAFSTAGSVGADSYTWALPSTSTITSGQNTSSILADIGTNNGTITVMGVNQCGN
                     SGTRTYAMTLNCREGFDKSELVQSVLLAPNPANDWVKLSFNSETAENYSIEVFDILGK
                     KVLSEGGISSKGLNEHSILINKLVKGIYIVSLQSNSVSSRQKLEIR"
     gene            7278..9827
                     /locus_tag="PKX59_04220"
     CDS             7278..9827
                     /locus_tag="PKX59_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOZ90104.1"
                     /translation="MAKRKNLQAEANTIDTVEEKPEVKRKTVKPKPEKLNLKWLANEQ
                     LHQIIGLFCILISIFLTIAFISYLFTWKIDFDKVKNGWWSLLTDRDLKVENWLGKTGA
                     LSANSFFYNAFGLSSFLFVFILFLSGFKLVFRHALLPFGRTIRISLFTLLFVSIACGY
                     IFKNTSLFLFTGGTFGFETSEWLQSTIGNAGTGLLLGFTLLTFSIITFKFKLKLPAIG
                     EKNIQQEEEDALLTEEQSENKINTISDEISKEAEDLKNNVPVSFELEPDMDKAISDLM
                     NSGDEQKKVPDSNNLELELNIETPEVEKDEPTVKESDIPLAIENLNVTEDATEASEEN
                     DNSELPDQVTLGEYDPTLDLSSYQYPVLDLLENYQSKSKLTHEELKAELETNKNKIVE
                     TLGHYNIGIEKIKATVGPTVTLFEIVPQAGVRISKIKNLEDDIALSLSALGIRIIAPI
                     PGKGTIGIEVPNQSPEVVPMKAVLGSEKFINSDMDLPIGLGKTISNEVFVTDLAKMPH
                     LLMAGATGQGKSVGLNAILVSLLYKKHPSQIKFVLVDPKKVELTLFKTIERHFLAKLP
                     GEEEAIITDTRKVVNTLNSLCVEMDQRYELLKDAQVRNIREYNTKFVARKLNPNKGHR
                     FLPFIVLVIDEFADLIMTAGKEVETPIARLAQLARAIGIHLIIATQRPSVNIITGTIK
                     ANFPARLAFRVSSKIDSRTILDCGGADQLIGKGDMLLSLGADVIRLQCAYVETFEVEK
                     ITDFIGSQRGYPDAFLLPEFVGDNESNTGEVDLADRDPLFEDAARMVVQHQQGSASLL
                     QRRLKLGYNRAGRLIDQLEAAGIIGPFEGSKAREVKFKDPIELETLLSNLK"
     gene            9936..10574
                     /locus_tag="PKX59_04225"
     CDS             9936..10574
                     /locus_tag="PKX59_04225"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOZ90105.1"
                     /translation="MTKKLMIFLVLLTKVSFAQKDSRSQEILKSVSAKYKTFKSVSAT
                     FRLNIEDKKAKTNQTQNGSILINGDKYNLSITGQQIISDGKTSWTYLKDANEVQVNDA
                     QNKTDGISPTNIFSIYEKGFDSRFAEEKTEGGKTYQYIDLKPLDKGKPYFKIQLKINK
                     AEKTIAGATVHNNNGSTITYTILKFTPNPAVKDSDFNFDAKKYPGVEVVDLR"
     gene            10664..11950
                     /locus_tag="PKX59_04230"
     CDS             10664..11950
                     /locus_tag="PKX59_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOZ90106.1"
                     /translation="MIHHDTVAVGAGLAGLSFAYHFDKNLPVFEALDTVGGLVRTIEY
                     KNCKFDLAPHLLHLRNPYVRELVFDKLGLEVENHRRKAHIYYDKTIIPYPFELNLLNL
                     SEQTKKDCIEGLDEVKSYNKEEEAEIRKGSYRDYALKCFGKGIANHYLLPYNRKIWDT
                     EPSEMTCEFMSHIITADKEQIRKNAFEDNTDSFGYNVEFYYPIHKGIQDLADIFARQL
                     TNIKFNTSAIFFNTRNKTIEFSDGSIVKYNKAVSTVPLQTIILNSDRDDLKALAKELV
                     FTSVYTVNLVIRGTLPDTHWMYFPDKDLSFYRISFPKNYFKKSTPADEQIIAVEVGSR
                     NHNLSIEEIERKVVEEIKQMPIFKIDELVFVHSIKIPVAYCIYEPKRPAIINKLMSEL
                     EEAGIYQSGRYGKWEYAGMQDAIMDGKVLSEKLKSL"
     gene            11947..12651
                     /locus_tag="PKX59_04235"
     CDS             11947..12651
                     /locus_tag="PKX59_04235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ90107.1"
                     /translation="MTENCSIVIPVFNEEEILEKRIHLLVEDLKKRFKDFEIVLTENG
                     SVDNTKSIIRTLSEKYDMVVAEIDDEKADYGQALINGINRARFEYITIFELDYLDMVF
                     LQKSNELLSSYDLVIGSKKLSGDLDRRPFKRKLFTWLYNTGLKVAFSYKLTETHGLKS
                     FKKSKLAPITNGCVTRHAVWPSEFCIRASRDKNLKVTEIGLSMPIEEIRTTRIKAIKR
                     LKKTIDDILLLRRSIN"
     gene            12663..13634
                     /locus_tag="PKX59_04240"
     CDS             12663..13634
                     /locus_tag="PKX59_04240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ90108.1"
                     /translation="MAENYHYHSYTPSDLDEILALFSLSFNGLKLSSEYYKWRVLNTP
                     VNKNLIDLFKNDNGKIISHYAVCPVKSFYKGETSLSELSLITMTHPEFKSLGLFPKLA
                     AKLYERSFKEENIQLVYGFPNTNSHYSFVKKLAWNDVYMIPTMKKTFTTAVQGDKTYT
                     EIFNADQRFDQLWEQHTQKADRFYANVRNSTFINWRFFNRPDKKYRLFAFENNSNIQG
                     YFVIKEYANASTKEIDVVDFLFTHDFDTSVFFNSIAQLATELDAKTANMWIPYNTPLY
                     NLAEKSGYLPKEHITYLGYKPFNPGKNLDELRIPTNWYLTMCDSDVY"
     gene            13668..14297
                     /locus_tag="PKX59_04245"
     CDS             13668..14297
                     /locus_tag="PKX59_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOZ90109.1"
                     /translation="MKNVLVVGAHPDDLEWMAGGTVAKIISEGGKVHLITLTNGTWKD
                     ASGNYYRDKDIAISEAKKAASVLGYSIEHLDEPCLDVNFRDELVVQVLNRIDKINADT
                     IICPWIDDLHHDHEMTARIAVSASRRVPRVLMGMCNWYVFRTPFSPNFFVDISDTYSK
                     KMAALECYESEMKRIGSTWRKYHDNITSNYGLMVNVERAEGFISYKYKL"
     gene            14422..14928
                     /locus_tag="PKX59_04250"
     CDS             14422..14928
                     /locus_tag="PKX59_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90110.1"
                     /translation="MFIAFNASAQRKVRKPIGYGASAIYNFQAEGIGADLRVRIPTPV
                     NNLYVVPEISYYPPFNRYHELYAGGALQYDILRIRSYNFYLLGAAYYNFWINADDYAP
                     EGKKKHNFAPEAGGGLVRNRGCWRPFIENRYDFKWKEDNLRIGIYYYPGQCRKNTRRE
                     KCPTVKGI"
     gene            14943..15854
                     /locus_tag="PKX59_04255"
     CDS             14943..15854
                     /locus_tag="PKX59_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90111.1"
                     /translation="MKSNKLFIYLTAIMLITVVACKKDRVPQDDYQNMDSFYSDHEEE
                     EQEYTIDTNGTCPLVCKKGTKICVNGEMFELNGGGQVTYPFQLKVIELYSIKDMILRR
                     IPSVASGNPLETSAEIRVRAFKNGNEIFLKPGKKYFLETAALPSINSAMQSYYGYSSG
                     NSANNWTNTISNFIPGFVDTLSSISPTSNSYVTTPATTGWFSPALLHQATSTTTITCS
                     VTGTNTQNLQVYVSFKGFKGVMQIQNLSGTNFPVGENVTLIVFGKKQNNQYVLDQQSF
                     TIAANQQIIFNLQDVSENDILNALAAL"
     gene            complement(15867..16889)
                     /locus_tag="PKX59_04260"
     CDS             complement(15867..16889)
                     /locus_tag="PKX59_04260"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOZ90112.1"
                     /translation="MIRKVFVPALILILLVSGFYYRHWLVYGFNQLKGQLNIIYNAQP
                     IGEVIENPLTPDSVKTKLILIRAIKKYAVDSLGLSETNNYETYYDQQGKPALWVLTAA
                     PEFKMVPYKWKFPFLGSLSYKGFFQKQKGVDELMLFRKNGFDADLSTVSAWSTLGWFG
                     DPVMSSMMRRNIGSLAELIIHEMTHTTLYIKGNVEFNENLATFIGEIGAEKFLTHKFK
                     DDSIVMTNYINHRNDNRIFSEYVIASVSKLDSLYSDKAFISLTYPKKLRLKYQKIASI
                     VLHIPKLPLKNDQRFEWNLQNKKLPDNTWFLSYSDYHALQPEINRVFRDSCNSSIQEL
                     IKYYKR"
     gene            complement(16912..18705)
                     /locus_tag="PKX59_04265"
     CDS             complement(16912..18705)
                     /locus_tag="PKX59_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ90113.1"
                     /translation="MSAIQEKNQSVKGGEFIIKESSAENTFIPEEFSEEHLMIEQTAN
                     DFLKQEVHTVLDRIDAMEAGLMPSLLDKAGELGLLGVSVPEQYGGFGKDFITGTLLTE
                     TLGAGHSFSVAMAAHTGIGTLPIMYFGNEKQKEKYLPLLASGQWKASYCLTEPGSGSD
                     ALAAKTKAILSSDGKHYILNGQKMWITNAGFADLFVVFAQVDGEKFTGFIVEKTYPGL
                     SLGEEEHKMGIKGSSTRQVFFSDCKVPIENVLGEIGKGHLIAFNILNIGRLKLCAAAL
                     GGAKHVSTHSVSYANTREQFKLPIAKFGAIRYKLAQQAIRIYACESALYRTAFMIDEL
                     EKQKLAEGIEYSQALLQAAEEYAVECAILKVDGSEALDYVADEGVQIYGGYGYSADFP
                     MDRSYRDSRINRIFEGTNEINRLLTVDMILKRAMKGKIDLMTPAMNIQKELMAIPDFS
                     EPDMSLFAQEKKFISNYKKALLAVAGSAVQKLMMTLAKEQEILMNIADMAIAIYISES
                     ILLRTEKLVNLKGEENCSVQLDMMRVYVNDAADRIALAGKEAINSFAEGDEQRMLLMG
                     LKRFTKVPSFNVKEARRRIAARLIEKNEYCF"
     gene            18852..24665
                     /locus_tag="PKX59_04270"
     CDS             18852..24665
                     /locus_tag="PKX59_04270"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ90114.1"
                     /translation="MHFTVKLAKINFLVGGNSALNKMSGMWLTALVIIFSLHQSIAQL
                     PVASFGISQSSGCAPLTVSFNNTSTNATSYHWDFGNGAVSVLNSPVITFTTAGNYYVK
                     LIATNSSGSDSVISSQPIHIENKPVVQFTLNKSSACQGEIINFTNQSAIFDSCQWDFG
                     DGVTSNQTNPTHIYATAGTYNVTLVLFRLNSTCTQTLTKNNIIIIKPIPIVTATVDTF
                     STCNINKIFHFNAGANTTSSYQWNFGDGGTATGAIVQHQYSQSGNYQVAVTASASGCS
                     STVNLSSPITVLNNPIPIITSNITSGCNPFNVVLSTNTTGTSSLLWNLDNGQTSTLST
                     VGVLYNNAGNYNPYVTANYPNGCSSQSAPLTINVLQSPVSTFTTSNTSGCKPLSISFQ
                     TTSCTGCSYQWSFGDGNSSTQLNGIHNYTQSGTFYPALTVTAANGCTATSNLPYGITV
                     NGPEADFLADKVTGCNPLTVNFANTSTGSTSWLWQFGTGDTSTVNSPTYLYQNQGSYN
                     VTLIASDGNGCKDTLRKQGYINPGPSVNNFSNPQPVSGCAPLTVSLSDSSGANSWLWD
                     YGDGSTATAQNVSHTYTTPGTYTVSLQTQSNGSTCSQTISNFATYIVGGGIANFTYNQ
                     TLCPPFIGTFTDSSLNAVSWLWNFGDGQTSTQQNPVHTYNQTGSYNVSLTITSANGCT
                     STKVEQYAMNFLTLGAAPSAHTLDTIPPLDVQFSANSVGATSWLWTFGDGDSSTLENP
                     MHTYQSTGPFTISLTIANDSCSYTYSFPPTNFGSGGTFYDENHDTTQFTMHYDGCAPL
                     TISFNNPFLNVVGSYWDFGDGNTSSQQNPVHTYNVPGIYDVMLIANLSNASVDTIYHS
                     QAVKVAGTPVTFIVNTVNTCNGSNIQALPSNLNLQSYNWDFGDNQTSSQVSPIHSYAN
                     TSNYIISLQAQDTSGCTVIHSKSLYANADANAILSNKNRACASDTITFSVQNTNYVNY
                     TWDFGDGTSATGNPIAHTYSDSGSYSVQVVITDINGCTQTVNASQQITIVKSVADFNI
                     NMLSSSCTMVNVQLTNTSTGSTSYTWDLGDGTMSSLTDVNKYYYYGPNPPYTYDIKLT
                     AYNNGCASTKTLAQAVTIPDFDVKFSYSRSQGCLPITINVTDSSLNAVAWKWSWGDGD
                     TTFTQATQHTFTSLPTDKIKLFAKDINGCEKNYELDNIQLPVAEFTQSDTIGCNPKTI
                     QFTDSSKNAHQWLWKFGNGSTSITPNPSINYSSTGNFIPSLIITDSFGCKDTVGGKII
                     SITEPQADFSYTKSSGCVPVIVQFQNTSSGAANYLWDFGDGSTSTLLNPNHIYTTGGN
                     YQVTLTVTDASGCSNTKTLSNALHIPGPLASFSLSNNHGCQPLTISTNDSSQNALSHF
                     WSFGDGDTSVATSPIHTFNTAGMYLITLIVKDANGCQSVYTHPDTIKVYNKPVAAFAI
                     SDSTICVGTSISLLNQSQSSSSYKWQFGDGTVSNQSNTSHQYQIPGQFSITLVASNGH
                     CSDTANSINAVTVIPFPIADFGGSPLIGCAPLSSNMVNISSSLQNPAFIWDGGNGLTS
                     SAFNPQFNYQNAGYYTVTLKVTNEGLCSDSITKLNFIAAQNNQPITPSEINYLTVLDS
                     NAIRISWQPSSATNLNRFELYRSTDDKNYNLIYTDTNTHNSTLNLQLAFIDNNVTTNQ
                     NRYYYKLQTINNCNNSYPLDSSISHASILLTTEPDTMSNMLSWLPYIGKTISQYSIYR
                     STDGLNYDLIDEIAGTEDFYIDSTAYCPQEYSYKVEAQLDSSIYTSESNNDASVPTSF
                     VNQITTSIIRSTVVDNNSILTEWHEGNPFPELVYYYLIYKSSDKDPGNFVLIDSVNNS
                     TKYYLDRDVDVNATNYQYKIEVENICQQRTGLTDLSGNILLQGKLDDMNNSRLEWTPY
                     KGWKEGVERYEIQKQDENGNWKTIKSVDGKTHHMEEE"
     gene            24737..25252
                     /locus_tag="PKX59_04275"
     CDS             24737..25252
                     /locus_tag="PKX59_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOZ90115.1"
                     /translation="MQNKKILITGYSQCGKSSIVNRLVNNRFHHSYSEDSQLCIARKE
                     LAINKKKINFVIWTAPGSVSNLKSFKSYYLGTDALIHVVDVNNPSTFSDLDAWISHYQ
                     KFLPGTPVYLTCNKIESNINLDCSDFFTQFDCYRTFFTSAKNNFMINEMFQTIAADLL
                     NEPFFQKIKIY"
     gene            25254..25652
                     /locus_tag="PKX59_04280"
     CDS             25254..25652
                     /locus_tag="PKX59_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90116.1"
                     /translation="MKNVHSKSQIIIFDNYGNILKSCDTIFQVSDLIGKNIMKQFPLL
                     NCYEPEIRNLSKSKEPFFVPCIAFSFANFKSICDFVFIRHEELNEITISWMISDNYVH
                     FKERLNKPINLPQTKSKPMVTEFVNRWHGK"
     gene            complement(25660..28773)
                     /locus_tag="PKX59_04285"
     CDS             complement(25660..28773)
                     /locus_tag="PKX59_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOZ90117.1"
                     /translation="MGNLKIYQSSAGSGKTYTLVKEYLLLVLKSPAALKGTLAVTFTN
                     AATGEMKERLILALWQLATGRNEKLKSDLASSLGTSFNIEQAAARVLNYAIHHYADIS
                     ISTIDSFFLNITRSIAYESRLPGRYEIDLNTEPAIDFMVQNILLAAGTDVGITNWLID
                     FAMYRVDSNKSWNMQGELRSIAKEIFKDHLKVNFETDVLADTEGINEVKSLYAAIWNK
                     LSGMAKKFLEIADSAGVEPIDFYYGRGGFHSFIVKIAAKVHPKDSTIGQRTWQILDRE
                     DVFADNAQDFLKQDVSLNGELLSLVRQIVDYYITNIERLLSAEVVTANIYQAVILKYL
                     FRQLKIYRKDHQILLMSDVGKILNSIIGNNDTPFIYEKAGNKYRNFLIDEFQDTSQVH
                     WKNLLPLLINSLSEQHQVLTVGDGKQSIYRWRGGDMNLLLNEVESTLKNHANSTDIFR
                     LDSNFRSSVSVVEFNNIFFSAINEIVSADSQQSQLLKKAYQADVVHQVAKRSEEKGFV
                     SIELYLDKEEYPELNYPEYFSEADKVVASHTLSYIQSQIEKGYKPGDIALLVRTNVKG
                     NMLADFLVLNGYNNINSRDSLLICHAPQIRFLINCLRLNADNKNQVAEKHILYFVSKH
                     LRMETKLIETNIEKYVKHVKLYSLTEFCAQVISKFGLNRQPDAYLQCFEDLIFEKSSK
                     GISTTAEFLDWWDVESNQSKISVQVPADENAITIITIHKSKGLQFPIVIIPYTDWDLN
                     SNKGLIWIRSDISPFDKLGAFPVRPSKKLLKTYFGQDYILENREKVLEEVNALYVACT
                     RAEDKLFILISGSGEEGEDGINKVNTLIKSAVMNAETVSSTLTKASDTVYTLGVDSEY
                     MQKRKKLSDTPDSFTNKELVNYFTSVNRISTGFKIKKYAEQSKSRLIGIKVHELLADF
                     RSESDLKAIEKHLSNLQLPDDEKNELLNGVQTTCNFLSEKKWVSPHFKIYNEHEMADA
                     EGNILRPDRLMIEGDNAVVIDYKTGAIETSHVQQIANYKEVLLDAGFKDVKTFLYYLE
                     GKELVEA"
BASE COUNT         9618 a         5421 c         4870 g         8941 t
ORIGIN      
        1 tactacgtta aagcagttta cgacacgcta aatgtttatt ctaccagtaa tgaagtaacc
       61 ttaattcagg aaccatcagt ttttacgccc aactcattta caccaaatgg cgatggtttg
      121 aatgaaacct ggtcaccggt atcgtcattc ataaaaaatt ataatcttaa aattttcaat
      181 cgttggggta atttgatttt tgaatccaac gaatctaatc gccaatggga tggcagctat
      241 caaggtcaac ctgcaccgct tggtatttat ttctacgttg tcacctatat aggttttcct
      301 gacaacacta agttcaacaa acggggctca gtgactttgt tacgctaaag gagttttcaa
      361 caataacatt tcaatggcat tctgtgtaac attagccatt aattttgtag catcattatg
      421 caggaaattg aaagtttaag tcccaaaaaa tatataatca taaaaggtgc atcggttcac
      481 aacctgaaaa aagtagatgt agccataagc agaaatagtt ttacagtgat taccggatta
      541 tcaggttcag gtaaatcgtc cttagctttc gatacattgt atgcagaagg gcaaagacgt
      601 tatgtagaaa gcctgtcaag ttatgcaagg caatttatgg gacgtttaga taaacctgag
      661 gtagaataca ttaaaggcat tgccccagcg attgcaattg aacaaaaagt aaataccaga
      721 aatccaagat caactgttgg cacttcaaca gaaatttacg attatcttaa attactattt
      781 gcacgaatag gaaaaacata ctcacctgta agtggtaaat tggtaaaaaa gaatactgtt
      841 gctgatgttg tagatatctt gaaaacacaa aatcaaaaca agagatttat cattgcagcg
      901 cctttgcaat ttcatcccgg aagaacttta catgaagaat taaatgcatt aatgcaaaaa
      961 gggtttagtc ggattgttgt tgacgataaa atgttgcgaa tagatgagta tgcagaacaa
     1021 atagaaaaaa cagatgcaca gcctgctgtt aaaacaagag gaagaaaaaa gaaagttgaa
     1081 tctattgaac caacaatcaa tacaaatatc tttatagttg tttcaagaat aattatccct
     1141 gaaaatatca atgaagaata tgaatcttct acagcagatt ctgttcagac tgcatttaat
     1201 gaaggtagcg ggcgttgcac tttaattgat ataacagacg aaacgctgca cgttaccaac
     1261 tttagtaatt acttcgaaga agataatatc cgttttgaag aaccatcaac acaccttttt
     1321 agctttaata atccttacgg agcatgtaaa aattgcgaag gctttggaag tgtaatcggc
     1381 attgatgaaa acttggttat cccaaacaaa acactttcaa tttttgatgg tgctgtagct
     1441 tgttggcgag gcgaaaaaat gaatgaatgg caacaggaat ttattttaaa agcatacaaa
     1501 tttaatttcc ctgtacatcg tccatattac gaattaaatg aaacggacat caagttatta
     1561 tgggaaggta ataaaacaat taaaggatta aatgactttt ttaaatatgt agaatcgcaa
     1621 tccttcaaaa ttcaatacag agttttacta agccgctatc gtggaaaaac aacttgccca
     1681 gaatgtaagg gaacacgttt acgtgccgat gccaattatg taaaggtgaa tagtaaatct
     1741 attaccgata ttgttttaat gccggtaaat gtcagtgttg aattttttaa taacattatt
     1801 ttagacagct atgaacaaaa agttgcaggg cggttattag ttgaaataaa aaacagattg
     1861 caatatttaa atgatgttgg gttaggatat ctgacattga acagactttc aaaaacactt
     1921 tcgggaggcg aatcgcaacg tataaatctg gcaacatcat taggcagcag tttggtagga
     1981 agcatgtata ttttggatga gccaagcata ggattacatc cacgagatac acagcgtttg
     2041 atttatgttt taaaatctct tcagaaagca ggtaatacgc tgattgttgt tgaacatgat
     2101 gaggagatta tgcgcaatgc tgatgaactt attgacatag gcccctttgc aggtacacat
     2161 ggcggcaaaa ttgttttcca aggaaatttt gagcaattag aacatagcac agaaagtgta
     2221 acagcaaaat atcttaccgg caaagaattt attcatgtgc caagtaaaag acgaaactgg
     2281 actaacagca ttgaaataac cggtgcaagt gaaaataatt tagcacacat tgacgctaaa
     2341 tttccacttg gtattctgac tgctgttact ggtgtcagtg ggtcaggtaa atcaaccttg
     2401 gtaaaaaaag ttctttatcc ggcacttaaa aaagttttag gtggctatga tgatgaatca
     2461 gggaaatttg aaaaactaac aggcgacttt acaacactga atggtgttga aattattgat
     2521 caggacccga taggtaaatc atcaaggagt aatcctgtta cctatgtaaa ggcttatgat
     2581 gaaatcagag cattgatggc cgacactcct tttgcaagac aaaaaatgat gaaaccatca
     2641 ttgttttctt ttaatgttga tggtggaaga tgtgagatgt gtcagggtga aggtgtagta
     2701 accattgaga tgcagtttat ggcagacata cagctaaaat gcgaaacttg ccaaggtaaa
     2761 cgctttaaga atgaaatatt ggcaatcacc tatcgggaaa aaaatatttt cgacatccta
     2821 tcaatgacgg ttgatgatgc cattagcttt ttttctaaag aaactgatga acgttatatt
     2881 ctttttacta ataaaataat tgccagacta caaccacttt cagatgttgg cttaggttat
     2941 atttctttag gacaatcctc tagtactctc agtggcggag aagcgcaaag aattaaatta
     3001 gcatcgtatc ttggcattgg tcatgcaaca acaaaaacac tttttatttt cgatgaaccg
     3061 actacaggtt tgcactttca cgatatcaga aaactacttg atgcatttac tgcattaatt
     3121 cgcaatggca attctatttt ggttatagaa cataatatgg atgttattaa atgtgctgat
     3181 tgggttattg atatggggcc gggtggtgga aatgaaggtg gtaaaatagt ttttgcagga
     3241 actcctgaag ctttgagtaa ttgcaacgaa tcttatacag gaaaatttct ctcagaaaaa
     3301 ttaattgccc aacaagagct ataactttac ttctgtgctt ctacatagag tgaaataccg
     3361 ggtaatgtct catcgaaaaa attaagatca cgcataacag gtgagtaact ttgtccataa
     3421 ccggaacgat atatactttt aaatccggct gcctttaaca ttctaataag cttttcctct
     3481 gtaaaccagt taatgtgatt acccgtgtat ttcttttgta cttcaatatc gcataatgaa
     3541 gtacaataat ccagagcatc ttcaaaggat ttctcttcaa acaatttttc taattcacta
     3601 tcggagatac gttgcttagc tccatggagc ggaatttcag atgcctgtgc tgcaaaatct
     3661 tcaagaaaaa tttgtgctag gctttcctga cttaaaggtt gcctgagatt tactctttta
     3721 taattttcag gcttactgaa ccattctatc caaaagaaaa aatgcctgtc gttttcttta
     3781 taggctctgt atgataactt aatatcctgt gtgacaactc taactcttcc tccgggtttt
     3841 aatactctga aagattcgtt aaacaaattc tgaaccgctg cattatttac atgctcaatg
     3901 gtatgactgg tatataccaa ctctgcagaa tctgtttcaa ttggcaatgg ctctaatgca
     3961 aataaatcat agttaatgcc ttttaatgta ttgtttgtat tatcggcata ccaatcattc
     4021 acataatcaa cattggtcca atacttatga ttaaatggcc ctgcccctac gttataaaat
     4081 cgcttatacg caagactatc attattaaat aaactcttat aaagttcttc tttataatca
     4141 gtcttaggta ttttagttac catcaaacca actacacttg caacagattt tactatgttt
     4201 ataatcataa atatttattt aaattgcaaa gtcgcttttt taaagcgact ttgcaattac
     4261 ctccaataaa taagactatc ttatctcaag tttttgtctg ctcgagacag aattactttg
     4321 caatgaaaca atatatatac ctttcactaa tttattaatc aaaattgaat gttcattcaa
     4381 tccttttgat gaaattccgc cttcgctaag gactttcttt ccaagtatat caaatacttc
     4441 tattgagtag ttctctgcag tttcagaatt aaaggacaat ttaacccaat catttgccgg
     4501 atttggagct aacaatactg actgtacaag ttctgattta tcaaaacctt cacggcaatt
     4561 aagtgtcata gcataggtac gtgtgccact gtttccacat tgattaacac ccattactgt
     4621 tatagttcca ttatttgttc caatgtcagc taaaatacta cttgtatttt gtccggatgt
     4681 aattgttgag gtacttggca gtgcccaagt atatgaatca gcacctacac ttccggcagt
     4741 tgagaatgca acaccggttt ggttagcaca gaaaactgat ggatttgcta caatacttgc
     4801 tggtctttca ggaatagcac gcacctggat gcaacgatat gttccggttc tacaagcatt
     4861 attacctgca acacaaagac tatatgtacc gcttccggga ttttggaagt caactgtaac
     4921 agtatttgta aaatttccta aaattattga agagttaggt ggtaaagtcc atttataact
     4981 tgttgcatgg ttagaatttc ctgcagtata aactaattgt gttttacaag taccatatac
     5041 ctgaccactt aaggatgttg gaactgatgg catatcaatt actgttgcca actttttaac
     5101 tggtgttgta aaacagatat tagatgcagt aactgttaca tcaccaccag tgtaggttgg
     5161 cccaaagtcc acaacaattg cattagttgt ttgtccacta actaaggttg ctccggcagg
     5221 aatggtccaa ttatacgaag atgctccagc cactgcaggt gccatataat ttacacccgg
     5281 taaattctca caaactcttg cagggcctgt gattgtaggc gttgatactt tatagcgagt
     5341 ccaggaactg gcagagtcta aactttgtcc acattcattt acagcataaa caaaaacaga
     5401 gtatccggaa tttattgttg aatcaaaagc taaagtaaca gcatcagtac cttgaccatt
     5461 taatatttgt acaccatcag tatatgtcca caaataactg gttgcatcca tttgtaatgg
     5521 aacagaataa attattccag atgtgccggg acaagttgtt gcctgaacac cacttattgc
     5581 acccggcttg gcagggactt gcttaatatc taatgtagct gtattagatg tgattgatga
     5641 tccacaatta ttggatacaa tgacatcata ctgatcggca tccgtactat cagcaccggt
     5701 aatatataat gtatcactgt ttgctccgga aattttacca ccattactta atggagcacc
     5761 atttttacgc cattggtatt gcaagttcat tcctaatgct ccaacagaaa acattgcttg
     5821 cgtactgttg caagtttcga aatcctgagg ctgttcgctt aaagcaatat tttttgaagt
     5881 tagtaaacaa cgcggacttc ctgttgcgca cgcattagtg atagtaacac aaattttacc
     5941 tgaaataaat gtatttgggt aatcaatatt aatattgttt gttccttgtc cggttaaaat
     6001 agttcccgga cctgttaatg tccaattata tgaaaccgca gttggactag aaggtacagt
     6061 ataaggagtt gttccattgc aaattcctga tgcaagacct atgataggtt gcggtgtttc
     6121 cggagttgct ctgacacgca cacttctgtt agaacttgaa ccgcatccgt tatttgcttt
     6181 cacataaata tcggtatagt tgtagttaat tggaaaagtt atctgaacgg tatcatttcc
     6241 ttgtcctaca agagttacac ccggagccac agtccatgta taggaagtta caggtacccc
     6301 atttgacgga ccggtagaat aagtcattgt tcctgtacat acctctgtcg gaccggtaat
     6361 tgctgttggt gtaccgggaa cccctgcata aattgtcatg cagcgaaatc cactccaagc
     6421 acatccgttt cctacacgta cacaaatatt tcctccatta aacccggcat tatattgcac
     6481 agtaatactg ttggttcctt gtccggtaag aagtgttgca ttattcggaa tagtccaatc
     6541 gaaaactctg tttacctgat tggtgacaga ataagtttca gtcactcccg gacaagcaaa
     6601 aatagaacct gaaatagttg atgcccctga tggtgcacct gcaggtgtca gcgctttgca
     6661 atatggtgct gaattaacac aaccattata agcagttaca cagaggcttc caggagcaga
     6721 aaataaagga ccgtaatcta ctgtaacaac attcgatgaa gttgtgtaag gattttgtcc
     6781 gctgatagtt gcacccggag gcaagttcca cgtatagctg atggcattag ttacaggtgt
     6841 aagggtgtag gttagcgaca ttccggggca tgctttaaaa tcgcctgtca ttccggaaaa
     6901 cgctcctaat ggagggcatt gtgctgatga taaattaatg ctcattacag caacagttac
     6961 taaagtcaaa aagactttac tcattctaat tagtgttgat tttgcatttt tcataataat
     7021 atgcggctat ttgatttgaa ttgtttacgc aataaattgg ttaaagtttt agtgatgcat
     7081 ttctttatgc aatcttacca atgctttagt aatacaaaaa tgcattacaa aataggttga
     7141 ttttatgaaa actatccaca taacaaccca tttatccaca ataaaatgtt tattacatgg
     7201 ttttgtttac tgtaatgtca acttagttta gatttaattt agctcaatta aatattttct
     7261 ttcacataaa aaacaaaatg gccaaacgca aaaatttaca ggctgaagca aacaccattg
     7321 acacggtcga agagaaacct gaagtcaaaa gaaaaactgt aaaacctaaa ccagaaaaac
     7381 tcaacttaaa atggttagct aatgaacagc tacatcaaat aatcggacta ttttgtattc
     7441 tgataagtat ttttctaacc atagctttta tctcttattt atttacctgg aaaattgatt
     7501 ttgataaagt taaaaatggc tggtggtcat tactgacaga ccgtgatctg aaagttgaaa
     7561 actggcttgg caaaacagga gctttatctg caaattcatt tttttacaat gcttttgggt
     7621 taagtagttt cttatttgtt tttatattgt tcttatccgg atttaaattg gttttccgac
     7681 atgctttact accatttgga cgcaccataa gaatttctct atttacactg ctctttgtct
     7741 caattgcatg tggatatatt tttaaaaata ccagtctgtt tttatttacc ggtggcacat
     7801 tcggatttga aacttctgaa tggctacaat ccacaatagg gaatgccggt accggattat
     7861 tacttggatt cacactactt actttctcaa tcattacatt caagtttaaa ttgaaacttc
     7921 cagcaattgg tgaaaaaaat attcaacagg aagaggaaga tgctttatta actgaagaac
     7981 aatcagaaaa taagataaac accatttcag atgaaatttc taaagaagca gaagatttaa
     8041 aaaacaatgt tcccgttagt tttgaattag aacctgatat ggacaaagcc atttcggatt
     8101 taatgaattc tggtgatgaa caaaaaaaag tacctgattc caataatctt gaattagaat
     8161 taaacattga aacaccagag gttgaaaaag atgagcccac agttaaagaa agtgatattc
     8221 cacttgcaat tgaaaatctg aatgtcactg aagatgcaac tgaggcatca gaagaaaacg
     8281 acaacagcga acttcccgat caggttactc ttggagagta tgacccaaca cttgatttat
     8341 cttcatatca atatcctgta ttagatttac tggaaaatta tcagagtaaa tctaaactta
     8401 cacacgaaga gttaaaagct gagttggaaa ccaataaaaa caaaattgtt gaaacacttg
     8461 gtcactataa tattggtatt gaaaaaatca aagcaacagt aggacctaca gtaactctat
     8521 ttgaaattgt accacaagcc ggtgtacgta tttcaaaaat caagaatctt gaagatgata
     8581 ttgctttaag tctttcagct ttgggtattc gtatcattgc ccctattccg ggcaaaggaa
     8641 ctattggcat tgaagtacct aatcaatcgc ctgaagtagt accgatgaaa gcagtattag
     8701 gctctgagaa atttatcaat tccgatatgg acctacccat cggacttgga aaaaccatat
     8761 cgaacgaagt atttgttacc gatttagcaa aaatgccaca cttgctcatg gctggtgcca
     8821 caggtcaggg taaatctgtt ggcttaaatg caattctggt ttccttgctt tacaaaaagc
     8881 atccatcaca aattaaattt gttttggttg atcctaaaaa agttgaactt actttattca
     8941 aaactattga acgacatttt ttagcaaaac ttcccggtga agaagaagca atcattaccg
     9001 atacacgcaa agtagtgaac acccttaact ctttatgtgt tgaaatggat cagcgctacg
     9061 agttacttaa agatgctcag gttcgtaaca tcagagagta caatacaaaa tttgttgcaa
     9121 gaaaacttaa ccccaataaa ggccaccgct ttctgccatt cattgttttg gttattgatg
     9181 agtttgccga cctgataatg acagcaggta aggaagttga aacacccatt gcccgattag
     9241 cgcagttagc acgagccatt ggtattcacc tcatcatagc aactcagcgt ccatcagtaa
     9301 atatcatcac cggaacaata aaagccaact tccctgcacg cctggcattc agggtttcat
     9361 caaaaattga tagtcgcaca attttagatt gtggtggtgc cgatcaactt attggtaagg
     9421 gagacatgtt attgtcgctt ggcgctgatg tgattcgttt gcagtgtgcc tatgttgaaa
     9481 cttttgaagt tgaaaaaatt acagatttta ttggctcaca aagaggctac cccgatgcat
     9541 ttcttttacc agaatttgtt ggtgacaacg aaagcaacac aggcgaagtt gatttagctg
     9601 accgtgatcc tttgtttgaa gatgctgcac gtatggttgt gcaacatcag caaggttctg
     9661 catctttatt gcaaagaaga ctcaaacttg gttataaccg tgccggcagg ttgattgatc
     9721 aacttgaagc tgctggaatt attggtcctt ttgaaggtag caaagccaga gaagtaaagt
     9781 ttaaagatcc tatagaactt gaaacattac tttcaaacct gaaataattc tgtttttaat
     9841 cctcctacat tattatattt gcaattgttc atgtgcattt cacctcgttt tgccttgtgg
     9901 cattgttttt attaatcaca tttaaaattt aagaaatgac aaaaaaattg atgatattcc
     9961 ttgtgctgtt gactaaagtt tcttttgcgc aaaaagacag ccgttcacag gaaattctta
    10021 aatcagtttc tgcaaaatat aaaacattca agtccgtttc tgcaactttc agactcaata
    10081 ttgaggacaa aaaagctaaa accaatcaga cacaaaatgg ttcgattctg ataaatggcg
    10141 acaaatacaa tctgagcatt acaggacaac agattatttc tgacggtaaa acatcatgga
    10201 cctacttaaa agatgccaac gaagtacagg ttaacgatgc acaaaataaa actgatggca
    10261 tctcccctac caatattttt tctatttatg aaaaaggatt cgactcacgt tttgcagaag
    10321 agaaaaccga aggcggtaaa acctatcagt atattgacct aaaacctttg gataaaggaa
    10381 aaccatattt taaaattcag ctgaaaatta ataaagctga aaagacaatt gcaggagcaa
    10441 ccgtacataa caacaacggt tctacaataa cttatactat cttaaaattc acaccaaacc
    10501 ctgctgtaaa agacagcgat tttaattttg atgcaaaaaa atatccgggt gtagaggtag
    10561 ttgacctcag ataaagtgtc ctcttttaag gtaattataa ggctttgaac gaaggataaa
    10621 aatccttact tttgcagcct tttaaatctc cttatttttc tacatgattc atcacgacac
    10681 agttgcagtt ggtgccggcc tggcaggatt aagttttgcc tatcattttg ataagaattt
    10741 accggttttt gaagcccttg acactgtagg tggtttggtg cggacaattg aatataaaaa
    10801 ctgcaaattt gatttagccc cacacttatt acatttacgc aacccttatg taagagagtt
    10861 agtatttgac aaattgggtc ttgaagttga aaatcatcgt agaaaagctc acatctatta
    10921 cgataaaacc atcatcccct acccttttga gctcaatcta ttaaatctga gtgagcaaac
    10981 caaaaaagat tgtattgaag gacttgacga agtaaaatca tataataagg aagaggaagc
    11041 agaaatcaga aaaggctctt acagagatta tgcattgaaa tgcttcggaa aaggtattgc
    11101 caatcattac cttctacctt acaacagaaa aatttgggac accgaacctt ctgaaatgac
    11161 atgtgagttt atgtcgcata ttattactgc agacaaagag caaatcagaa aaaatgcatt
    11221 cgaagacaac acagacagtt ttggctataa tgtagaattc tattatccta tacataaagg
    11281 tattcaggat ttagcagaca tttttgctag acaactcact aatattaaat tcaatacttc
    11341 tgcaatattt tttaatacac ggaataaaac aattgaattt tctgatggaa gtattgtaaa
    11401 atataacaaa gctgtttcta ctgttcccct acaaactatc atattaaaca gtgacagaga
    11461 tgacttgaaa gcattagcca aagaattggt tttcacctct gtttatacag taaatttagt
    11521 tatcagagga acactgcctg acacacattg gatgtatttc cccgataaag atctttcgtt
    11581 ctacagaata agcttcccta aaaactattt caaaaaatca acacctgctg acgaacaaat
    11641 tattgctgtt gaagttggca gtcgcaatca taacctgagt attgaagaaa tagaacgcaa
    11701 agttgttgaa gagattaaac aaatgccaat attcaaaatt gacgaattgg tatttgttca
    11761 ctcgattaaa atacctgtgg cctattgtat atacgaacca aaacgccctg ccataatcaa
    11821 caaactgatg agtgaactgg aagaagcagg aatttatcag tcaggtcgct atggaaaatg
    11881 ggaatatgca ggcatgcagg atgcaattat ggacggcaaa gtattgtcgg aaaaattaaa
    11941 atcattatga cagaaaattg ttcaatagta ataccggtat ttaacgaaga agaaattctt
    12001 gaaaaaagaa tacacctgct cgttgaggat ttaaaaaagc gttttaaaga ttttgaaatt
    12061 gtactaacag aaaacggaag cgttgacaac accaaatcta tcatcagaac actatctgaa
    12121 aagtatgata tggttgttgc agaaattgat gacgaaaaag cagactatgg tcaggctttg
    12181 attaacggca ttaatcgggc acgttttgag tacatcacca tatttgagct tgactattta
    12241 gatatggtct ttttacaaaa atcaaatgag ttgcttagct catacgatct tgttattggc
    12301 tcaaaaaaac tttctggcga tcttgacaga agaccattta aaagaaaact ttttacctgg
    12361 ctctacaata cagggcttaa agttgcattt agctacaagt taacagaaac tcatggactg
    12421 aaaagtttta agaaaagtaa attagctcca atcactaatg gctgtgtaac acgacatgct
    12481 gtttggccaa gtgaattttg cataagagct tcacgtgata aaaatttaaa agtaacagag
    12541 attggcttat caatgccgat tgaagaaatc agaactaccc gtattaaagc aataaaaaga
    12601 ttaaagaaaa ctattgacga tattttgctg ttacgcagat ctattaacta ataaaatacg
    12661 ctatggcaga aaattatcat tatcacagtt acactccatc agatcttgat gaaatacttg
    12721 ccttattttc tctttcattt aacggactaa aactctcatc agaatattat aaatggcgtg
    12781 ttctaaatac tcctgtaaat aaaaacctga ttgatttatt taaaaatgac aatggtaaaa
    12841 taataagtca ttatgcagtg tgtccggtaa aatcttttta caaaggcgaa acttcactaa
    12901 gtgaactttc gctcatcaca atgacacatc ctgaatttaa atcattagga ctgtttccaa
    12961 aacttgcagc aaaactttac gagcgctcct tcaaggaaga gaatattcaa ttggtttatg
    13021 ggtttccaaa caccaactcg cattacagct ttgtaaaaaa attagcttgg aatgatgtat
    13081 atatgattcc aacaatgaaa aaaacattta caactgctgt tcaaggtgat aaaacttata
    13141 cagagatttt taatgccgac caacgatttg atcagctatg ggagcaacac acacagaagg
    13201 cagatcgttt ttatgcgaac gtcagaaaca gcacatttat aaactggcgt ttttttaatc
    13261 gccctgataa aaaatatcgc ttatttgcat ttgaaaacaa cagtaatata caaggttatt
    13321 ttgtaattaa ggaatatgca aatgcatcaa caaaagaaat tgatgttgtt gattttcttt
    13381 ttacacatga ttttgacaca tcagtattct ttaattccat agcacaatta gcaacagaat
    13441 tagatgctaa aacagcaaac atgtggattc cctacaatac tccattgtat aatcttgctg
    13501 aaaaatcagg ctatttgccg aaagaacata taacttactt aggatataaa ccattcaacc
    13561 caggaaaaaa tttagatgag ttaagaatac caacaaactg gtatttgaca atgtgtgatt
    13621 ctgatgttta ctgataggtt actattgaat ttaaaatcaa tgacaacatg aaaaatgttt
    13681 tagtagtagg tgcacatcct gatgatttgg aatggatggc cggtggtact gttgccaaaa
    13741 tcatttcaga gggaggtaaa gtccatttaa taactttaac caatggtaca tggaaagatg
    13801 cttccggaaa ttactaccgt gataaagata ttgctatcag tgaagctaaa aaagctgctt
    13861 ctgttttagg ctactcaata gaacatttag atgaaccttg tcttgatgta aatttcagag
    13921 atgaattagt agttcaggta ttaaaccgca ttgacaaaat aaatgcagat acgataatat
    13981 gtccatggat tgacgattta catcacgatc atgaaatgac tgcacgtatt gcagtgagtg
    14041 caagcaggcg tgtgccacgt gtactcatgg gcatgtgcaa ctggtacgtg tttcgtacac
    14101 catttagtcc taactttttt gttgacatca gcgacacata ttctaaaaag atggcagcat
    14161 tagaatgcta tgaatcagaa atgaaacgca ttgggagcac ttggagaaaa tatcacgaca
    14221 acatcacttc aaattatggt ctgatggtga atgtagagcg cgcagaaggt tttatcagct
    14281 acaaatacaa attgtagttc tgtttataat caaataataa ttttccagta atgccgtttt
    14341 gggaatagat aaagttaaca tctttaatcc caaaatattt caatgtttaa aatcaggcat
    14401 atatccatta ttttgtttgc aatgttcatt gcgttcaatg cttcagcaca acgtaaagta
    14461 cgtaaaccta taggctacgg tgccagtgca atttataatt ttcaggccga aggtattggt
    14521 gcagatttgc gtgtcagaat tccaacaccg gtaaataacc tttatgtagt tcctgaaatt
    14581 tcatattacc ctccttttaa tcgttatcat gaattgtatg ccggtggggc tttgcaatat
    14641 gatatattaa gaatcagaag ttataatttt tatttacttg gtgctgccta ttacaatttc
    14701 tggataaatg cagatgacta tgctcctgaa ggaaaaaaga aacacaattt tgctcccgaa
    14761 gccggtggtg gcttggtgcg aaacagagga tgttggcgtc catttattga aaaccgctat
    14821 gactttaaat ggaaagaaga taatttgcga ataggtattt attattatcc cggacagtgt
    14881 agaaaaaata caagaagaga aaaatgtcca acagttaaag gaatttaatc aaactttgta
    14941 atatgaaatc gaacaagcta ttcatttatc ttactgcaat aatgcttatt acagtagttg
    15001 catgcaaaaa agaccgtgtg ccacaggatg attatcaaaa catggattca ttctacagcg
    15061 accatgaaga agaagaacag gaatatacca ttgacaccaa tggaacttgt ccattagttt
    15121 gtaagaaagg aactaaaatt tgtgtcaacg gagaaatgtt tgagttaaat ggtggaggcc
    15181 aggttactta tccttttcag ctaaaagtaa tagagttgta ttctattaaa gatatgattt
    15241 taagaagaat tccttctgtg gcttcaggaa atccacttga aacaagtgca gaaataagag
    15301 tgagagcatt taaaaacgga aatgaaattt ttctcaaacc cggcaaaaaa tattttcttg
    15361 aaacagctgc attgccatct ataaattcag ccatgcaatc ctattacggt tatagcagcg
    15421 gtaatagtgc taataactgg accaatacta tttctaattt tattcctggt tttgttgata
    15481 cactatcgag tatttctcca acttctaaca gttatgtaac aacacctgcc acaacaggtt
    15541 ggttttctcc tgcattatta catcaggcaa catcaacaac aactattaca tgtagtgtca
    15601 ccggaacaaa tacgcaaaac ttacaggtat atgtttcatt caaaggtttt aaaggtgtta
    15661 tgcaaattca aaacctgagt ggcacaaact ttcctgttgg cgaaaacgta acattgatag
    15721 tattcggtaa aaaacaaaac aatcaatacg ttttagatca gcagagtttt acaattgctg
    15781 ccaatcagca aattattttt aatcttcagg atgtttcgga aaatgacatt ctgaatgctc
    15841 ttgcagcatt gtaagtaaat tacgccttat ctcttatagt attttatcaa ttcctgaatt
    15901 gaactattgc aggaatctct aaacacccga ttaatttcag gctgcaaagc atggtagtca
    15961 gaataagata gaaaccaggt gttgtcgggc agttttttat tttgcagatt ccactcaaac
    16021 ctttgatcat ttttcaatgg taatttcggt atatgcaaaa caattgatgc tattttttga
    16081 tatttcagac gtagtttctt cggataggtt aatgatataa aagctttatc actatacaat
    16141 gagtccaatt tagatactga agcaattaca tattctgaaa atattctgtt gtcattgcga
    16201 tgattgatgt aatttgtcat aacaatgctg tcatctttaa atttatgagt cagaaatttt
    16261 tcagcaccaa tttcaccaat aaaagtcgca aggttttcat tgaattcaac atttccttta
    16321 atgtaaagcg ttgtatgagt catctcatgg ataatcaatt ctgcaagcga gccaatattc
    16381 cttcgcatca ttgaagacat tacaggatca ccaaaccagc caagggtact ccatgctgat
    16441 actgttgaca aatctgcatc aaagccgttt ttcctgaaca acatcaattc atcaactcct
    16501 ttttgtttct gaaaaaatcc tttatatgat aaagacccaa gaaatgggaa cttccattta
    16561 tatggtacca ttttaaattc aggagcagcg gtaagcaccc acaatgcagg tttcccttgt
    16621 tgatcataat aagtttcgta gttgttagtt tctgataatc ctaaagaatc aacagcatat
    16681 ttttttattg cacggattaa tatcagtttt gttttaactg aatcgggagt taatggattc
    16741 tctattactt cgccaatagg ctgtgcgttg taaataatat ttagctgacc ctttagttga
    16801 ttaaacccat aaaccaacca atggcggtaa taaaatcctg aaactaataa aattaaaatg
    16861 agtgccggaa caaaaacctt cctgatcatc agcttagaac tgctctacaa atcagaagca
    16921 gtattcgttc ttttcaatta agcgtgctgc aattcttctt cttgcttctt taacattgaa
    16981 tgacggaacc ttggtaaagc gttttaatcc catgagcaac attctctgtt cgtcaccttc
    17041 tgcaaaagaa ttaattgctt cttttcctgc caatgcaatt ctatctgctg catcatttac
    17101 ataaacccgc atcatatcaa gctgaacact gcaattttct tcacctttta aattaacaag
    17161 tttctctgtc ctcaataaaa ttgactctga aatataaatt gcaattgcca tatctgcaat
    17221 gttcatcaga atctcctgct ctttagcaag tgtcatcatc aatttttgta cagcagaacc
    17281 tgcaactgca agtaacgctt ttttatagtt agaaataaac tttttctctt gtgcgaatag
    17341 cgacatatca ggctcactaa aatcaggaat tgccataagt tctttctgaa tattcattgc
    17401 cggtgtcatt aagtcaatct tacctttcat ggcacgtttt agaatcatat caacagtaag
    17461 gagtctgtta atttcatttg ttccttcaaa aattctgttg atgcgtgaat ctctatatga
    17521 tctgtccatc ggaaaatctg cactatagcc ataaccacca taaatctgca caccttcatc
    17581 tgcaacataa tcaagtgctt cagaaccatc aactttaaga atggcacatt caacagcata
    17641 ttcttcggca gcctgaagta atgcctgaga atattcaatg ccttctgcaa gtttttgttt
    17701 ttcgagctcg tctatcataa atgctgtacg atacaaagca ctttcacaag cataaattct
    17761 gattgcctgt tgcgcaagtt tatatctgat ggcaccaaat tttgcaatag gcaatttaaa
    17821 ctgctcacgt gtgtttgcat aggaaacaga gtgtgtactt acatgttttg cgccacctaa
    17881 tgctgctgca cacaatttca aacgtccaat atttaaaata ttgaaagcaa taagatgtcc
    17941 tttgccaatt tcgcccaaaa cattttctat aggcacttta caatcactga aaaaaacctg
    18001 tcttgtactt gagcctttta ttcccatctt atgttcttct tcgccaagtg atagtcccgg
    18061 ataagttttc tctacaataa aaccggtgaa cttttcgcca tcaacctgtg caaatactac
    18121 aaacaaatct gcaaagcctg cattggtaat ccacattttt tgtccgttaa ggatataatg
    18181 ctttccatca gaagaaagaa tagctttagt tttcgctgca agcgcatcag aaccggaacc
    18241 gggttcagtt aaacagtaag atgctttcca ctgacctgat gctaatagcg gtaaatattt
    18301 ttctttctgc ttttcatttc cgaaatacat tattggcaat gttccgatac ctgtatgtgc
    18361 tgccattgca acagaaaatg aatgacctgc tcctaaagtt tctgtcagta atgtgcctgt
    18421 aataaaatct tttccaaatc caccatattg ttccggaacc gaaacaccaa gaagtccaag
    18481 ttctcctgcc ttatctaaca atgatggcat aagtcctgct tccattgcat caatacgatc
    18541 gagtactgta tgtacttcct gttttaaaaa atcatttgct gtttgctcaa tcatcaaatg
    18601 ctcttcgcta aactcttcgg gtataaaagt gttctctgca cttgattctt taataataaa
    18661 ttctcccccc ttaaccgatt ggttcttttc ctgaattgct gacataataa aagtggtttt
    18721 tttaacgcaa taaaaataaa aatattaaac ataaatacca gccttaaggt ttcaatattt
    18781 ctaaaatacc ggaaattaaa tctactgaaa aacaaacaga taatattaaa gtgtaagaat
    18841 tataactttt aatgcacttt accgtaaaac ttgctaaaat caacttttta gtgggaggaa
    18901 atagtgcttt aaataaaatg tcaggaatgt ggcttactgc cttagtgatt attttttcgc
    18961 tacatcaaag tattgcacaa ctaccggttg catcatttgg catcagtcag tcatcaggtt
    19021 gtgcacctct aactgtttca tttaacaata cgagtaccaa tgcaacaagc tatcattggg
    19081 attttggcaa tggtgcagtt tctgttttga atagtcctgt aattactttt actactgcag
    19141 gaaattatta tgtaaaactc atcgcaacaa attcttcagg cagtgattct gtaatttctt
    19201 cgcaacctat acatatagaa aacaaacctg ttgtacaatt tacactcaat aaatcttcag
    19261 catgtcaggg tgaaattata aactttacca atcaaagtgc aatttttgac agttgtcaat
    19321 gggattttgg tgatggtgtt acaagcaatc aaacgaatcc tacacatatt tatgcaacag
    19381 ccggaactta taatgttact ttagtcttgt tcaggctcaa ctcaacatgc acacaaacac
    19441 tgacgaaaaa taacatcatt attattaagc ctattccaat tgtaacagca acggttgaca
    19501 cattcagcac atgtaatatc aataaaatat ttcattttaa tgctggtgca aataccacat
    19561 catcttatca atggaatttt ggcgatggag gaactgcaac aggcgcaatt gttcaacatc
    19621 aatattcaca atccggaaac tatcaggttg cagttactgc atcagcctcc ggttgctcat
    19681 ctaccgtaaa tctatcttca ccgataacag tacttaacaa tcctataccc ataattacat
    19741 ccaatatcac atcgggttgt aatccgttta atgttgtttt atcaactaat actacaggaa
    19801 catcatccct tctatggaac ttagacaatg gccagacatc tacactatca acagttggag
    19861 tattatataa caatgcagga aactacaatc cttatgtaac cgcaaactat cctaacggct
    19921 gttcaagcca gtctgcacca ttaacaataa atgtattaca gtctccagtc tcaacattca
    19981 ctacatcgaa tacttcagga tgtaaaccat tgagtatttc atttcaaact acatcatgca
    20041 caggatgtag ctatcaatgg agttttggtg atggcaatag ctcaacacaa ttaaatggca
    20101 ttcataatta cacacaatca gggacattct atcctgcgct aaccgttact gctgcaaacg
    20161 gatgtacagc tacatcaaat cttccctatg gaattacggt caacggacct gaagccgatt
    20221 ttcttgcaga taaagtaact ggatgtaatc cattaactgt aaactttgca aatacaagta
    20281 caggttctac ttcatggctt tggcagtttg gcacaggaga tacttcaaca gtaaacagtc
    20341 caacctatct ctatcaaaat caaggcagtt ataatgtgac tctaattgct tctgatggca
    20401 atggttgtaa agacacatta cgtaaacaag gttacatcaa ccccggacca agtgtaaaca
    20461 acttcagcaa tccgcaaccc gtatcaggat gcgcaccact tactgtttca ttaagcgact
    20521 catcaggtgc aaactcatgg ctttgggatt atggtgatgg cagtactgcc acagcacaaa
    20581 atgtttctca tacatatact acaccgggaa catatacagt aagtctgcaa acgcaaagca
    20641 atggctcaac atgttctcaa accatcagca attttgccac gtatattgtt ggtggtggta
    20701 tagctaattt tacatataac caaacacttt gtccgccatt tatcggaaca tttacagaca
    20761 gcagtttaaa tgctgtttca tggttatgga attttggtga cggacaaaca tcaacacaac
    20821 aaaatcctgt tcacacctac aaccaaaccg gtagttacaa tgtttcttta accatcactt
    20881 ctgcaaatgg atgtacatca acaaaggttg aacaatatgc aatgaacttt ttaaccttag
    20941 gtgctgcacc ttctgcccat acattagata ccattcctcc tcttgatgtt cagttttcgg
    21001 ccaactcagt aggtgcaaca tcatggctat ggacttttgg cgatggagat tcttcaacac
    21061 ttgaaaatcc tatgcacacc tatcaatcaa caggcccttt cacaataagt ctgacgattg
    21121 ccaacgacag ttgtagttat acctacagtt tccctcctac taactttgga agtggaggaa
    21181 cattttatga tgaaaaccat gatacgacac aattcaccat gcattatgat ggttgcgcac
    21241 cattgaccat aagtttcaat aacccatttt taaatgttgt tggctcgtat tgggattttg
    21301 gtgatggcaa tacttcgtct caacaaaatc cggtacacac atacaacgta cccggcattt
    21361 acgatgtaat gctcattgca aatttatcta atgcaagtgt tgatactatt tatcattcac
    21421 aggctgtaaa agtggcagga acaccggtaa catttattgt taatacagtt aatacatgta
    21481 atggctcaaa cattcaggct ttaccatcaa atctgaatct gcaaagctat aattgggatt
    21541 ttggtgataa tcaaacaagc tcacaggttt caccaataca tagctatgcc aatacaagca
    21601 attacattat ttctttacag gcacaagata ctagtggatg tactgtaatt cattccaaga
    21661 gtttatatgc caatgctgat gccaacgcta ttctttccaa taaaaacaga gcatgtgcat
    21721 cagacactat taccttttca gttcaaaaca caaactatgt aaactatact tgggactttg
    21781 gtgatggcac ttcggcaaca ggaaatccta tagctcacac atactctgat tcaggatctt
    21841 attctgttca ggttgttata acggatatta acggttgtac gcaaacagta aatgcttcac
    21901 aacaaataac cattgtaaaa tcagtggctg acttcaacat caatatgctt tcctcatcat
    21961 gcaccatggt gaatgtacag ttaacaaata cctctacagg cagcacttct tatacatggg
    22021 atttgggaga tggaactatg tcatcattaa ccgatgtaaa taaatattac tactatggac
    22081 caaatccacc ctacacttat gatattaagc taactgcata caacaatgga tgtgcaagca
    22141 ctaaaacatt agctcaggct gttaccatac cagattttga tgttaaattc agttattcaa
    22201 gaagtcaagg ttgcttacct atcacaatta atgtaactga ttcaagccta aatgctgtag
    22261 catggaaatg gtcttggggc gatggtgaca ccacttttac tcaggctaca caacatacct
    22321 ttacctcttt accaacagat aaaataaagc tttttgctaa agatattaat ggatgcgaaa
    22381 aaaattatga gcttgataac atccaacttc cggtagcgga atttacgcag tcagatacaa
    22441 taggatgtaa tccaaaaacc atacaattta cagattcaag taaaaatgca catcaatggt
    22501 tatggaaatt tggtaacggg tcaacatcaa taactccaaa tccaagtatt aattacagta
    22561 gtaccggcaa ttttatacca tctttaataa ttacagattc ttttggatgt aaagatactg
    22621 ttggtggaaa aataatctcc atcacagagc cacaggctga ttttagttat acaaaatctt
    22681 caggttgtgt tccggtgatt gttcaatttc aaaatacatc ttcgggtgct gcaaactatc
    22741 tttgggattt tggagatggc agtacatcaa cactcttgaa tccaaatcat atctacacaa
    22801 caggcggaaa ctaccaagtt acacttacag taacagatgc atcgggttgc agtaatacaa
    22861 aaacattgtc caatgcttta cacataccag gacccttagc ttcattttct ctttcaaaca
    22921 atcatggttg tcaaccttta actatcagca ctaacgactc ctctcaaaat gcacttagtc
    22981 acttttggag ttttggagat ggagatactt ctgtggcaac ttcacccatt cacacgttca
    23041 atactgccgg tatgtactta ataacattga ttgtaaagga tgccaatgga tgtcaatctg
    23101 tttatacaca tcctgataca attaaagttt acaacaaacc tgttgcagca tttgcaatca
    23161 gcgactctac aatttgtgtt ggaacttcaa tttctttact caaccaatct caatcatcaa
    23221 gtagttacaa atggcaattt ggagatggta cagtatcgaa tcagtcaaat acaagtcacc
    23281 aatatcagat tccagggcaa tttagcatta cattagttgc ttctaatggt cattgttcgg
    23341 atacagctaa cagcatcaat gccgttaccg ttataccttt ccccattgcc gattttggag
    23401 gttcgccatt gattggttgt gcaccgcttt cttcaaacat ggtcaacatc agctcatcac
    23461 tacagaaccc tgcatttata tgggatggcg gcaatggact cacttcatca gcattcaatc
    23521 cgcagtttaa ttatcaaaat gcaggatatt atacagtgac attaaaagtc actaatgaag
    23581 gcctttgcag tgatagtatt actaaactca acttcatagc tgcacaaaac aatcaaccaa
    23641 taacaccttc agaaataaat tacctcaccg tactcgatag caatgcaatc agaatatcat
    23701 ggcaaccatc atcagctacc aatctaaaca gatttgaatt atacagatca actgacgata
    23761 aaaattacaa tctgatttac acagatacaa atacacacaa cagcacattg aatctgcaat
    23821 tagcatttat tgacaataat gtaacaacaa atcagaatcg ctattattac aaacttcaaa
    23881 caatcaataa ttgcaacaat tcatatccat tggatagttc aatatcccat gcatcaattc
    23941 tattaacaac agagcccgac acaatgtcaa acatgctctc atggttacct tatattggta
    24001 agacaatttc acaatactcg atttaccgaa gtactgatgg attgaattat gacctgatag
    24061 atgaaattgc aggaacagaa gatttttata ttgattctac agcatattgt cctcaggaat
    24121 acagttataa agttgaagca caattagaca gcagcatata cacttctgaa agcaataatg
    24181 atgcatcggt accaacaagt tttgtaaatc agataaccac atcaatcatt cgttctactg
    24241 tggttgacaa caacagtatt ttaacagaat ggcatgaagg caacccattt cctgagttgg
    24301 tttactatta tttaatttat aaatcatcag acaaagaccc gggaaatttt gttctgatag
    24361 attcggtaaa caacagcaca aaatactatc ttgacagaga tgtggatgtc aatgctacaa
    24421 attatcaata caaaattgaa gtagaaaata tatgtcagca aagaacaggt ctgacagatt
    24481 tatcaggaaa cattcttcta caaggcaaat tagatgacat gaacaacagc agattagaat
    24541 ggactccata caaaggctgg aaagaaggtg ttgaacgcta tgaaatacaa aaacaagatg
    24601 aaaacggtaa ttggaaaacc ataaaatcag tagatggcaa aacccatcac atggaagaag
    24661 aataattttc agaagttcaa acaaaatact gcaacctttt tctcttttct gcatataaac
    24721 actataaaca atcggcatgc agaataaaaa aatactcata acaggatata gccaatgcgg
    24781 taaaagttct attgtgaacc gattggtgaa taacagattt catcattcct attcagaaga
    24841 tagtcaatta tgcattgcaa gaaaagagtt ggctataaat aagaaaaaga tcaattttgt
    24901 tatctggaca gctccaggaa gtgtttcgaa tctaaaatcg ttcaagtcat attatcttgg
    24961 aacagatgca ttaatacatg tagttgatgt aaacaaccca tcaacatttt cagatcttga
    25021 tgcatggatt agtcattatc agaaatttct tccaggcacg cctgtttact taacttgcaa
    25081 taaaattgaa tcgaatatca atcttgactg cagtgatttc tttacacagt ttgattgcta
    25141 cagaactttt tttacatcag caaaaaacaa tttcatgatt aatgaaatgt ttcaaacaat
    25201 agcggctgat ttactgaatg agccattctt tcaaaaaatc aaaatttact gatatgaaaa
    25261 acgttcacag caaaagccag attattattt ttgataacta tggcaacatt cttaaaagct
    25321 gtgatacaat ttttcaggta agcgatttga taggaaaaaa catcatgaag caatttccgc
    25381 tgttgaattg ctatgaacct gaaattagaa atctttcaaa aagtaaagag ccattttttg
    25441 ttccttgcat agcattctct tttgctaact ttaaaagtat ttgcgacttt gtttttataa
    25501 gacatgaaga attgaatgag atcaccatat cctggatgat ttctgacaat tatgttcact
    25561 ttaaagaacg gttgaacaaa ccgattaatc ttcctcaaac taaatctaaa ccaatggtta
    25621 cagaatttgt caaccgttgg catggaaaat agaaagcatt taagcctcaa ctaattcttt
    25681 cccttccaga taatataaaa aagtcttcac atccttgaag cccgcatcca gcaaaacttc
    25741 cttgtaatta gctatttgtt gcacatgaga agtttcaatg gctcctgtct tataatcaat
    25801 aaccacagca ttatcccctt caatcatcaa tctgtcaggt cttaaaatat taccttctgc
    25861 atcggccatt tcatgttcat tataaatttt aaaatgcgga ctaacccatt ttttctcaga
    25921 gagaaaattg caagtagttt gcacgccatt cagcaactca tttttttcgt catccggtaa
    25981 ttgaagatta cttaaatgct tctctattgc ttttaaatca gattcactgc gaaaatcagc
    26041 cagcaactca tgaactttaa tccctattaa gcggctttta ctttgttcag catacttttt
    26101 aattttgaat ccggtgctta tcctgtttac acttgtaaaa taatttacca gctccttatt
    26161 agtaaaacta tcaggtgtgt cggatagttt ctttctcttt tgcatatatt ctgaatcaac
    26221 tcccaaagtg taaacagtat cacttgcctt tgtcaaagtt gaactaacag tttcagcatt
    26281 cattactgcc gacttaatta atgtattaac tttattaata ccatcctcac cttcttctcc
    26341 actacctgaa atcaaaataa acaatttatc ttctgctctg gtacatgcaa cataaagtgc
    26401 atttacttct tccaatactt tttcacggtt ttcaagaata taatcctgac caaaatatgt
    26461 tttcaataat ttctttgaag gtctgacagg aaatgcacct aatttatcga aaggtgaaat
    26521 atcacttctg atccaaatca aacctttatt actatttaaa tcccaatcag tataaggaat
    26581 gattacaata ggaaattgca atcctttaga cttatgaatg gtgatgatgg taatagcgtt
    26641 ttcatcagca ggaacctgca ctgaaatttt actttgattg gattcaacat cccaccagtc
    26701 aagaaactct gcagtagtac taattccttt acttgatttt tcaaaaatca agtcttcaaa
    26761 gcattgaaga tatgcatcag gttgcctatt caacccaaac ttacttatta cctgtgcaca
    26821 aaattcagtc agcgaataca gtttaacatg cttaacatac ttttcgatat ttgtttcaat
    26881 tagtttagtt tccattcgca gatgtttaga cacaaaatat aaaatgtgct tttctgctac
    26941 ctgattttta ttatcagcat tgagtctgag acaatttatc aaaaatctga tttgaggcgc
    27001 atggcaaatc aacaatgaat cacgagaatt aatattatta taaccgttca aaaccaagaa
    27061 gtctgccaac atgtttccct ttacattggt tctgacgagc aatgcaatat caccgggctt
    27121 atatcccttt tcaatctgac tttgaatgta acttagcgta tgtgatgcaa ccaccttatc
    27181 agcttcacta aaatattccg gataatttag ctcaggatac tcttctttat ccaaataaag
    27241 ttcaattgac acaaaacctt tttcctcact gcgttttgca acttgatgca ctacatctgc
    27301 ctgatatgct ttcttcagca attgtgattg ttgtgaatca gcagatacaa tttcattgat
    27361 tgcactaaaa aaaatattat taaactcaac aacagaaaca gacgaacgga aattagaatc
    27421 caaccggaat atatcagtgc tatttgcatg attctttagt gtactttcaa cttcatttaa
    27481 aagcagattc atatctcctc cccgccagcg gtaaattgat tgcttaccat cgccaacagt
    27541 aagaacctga tgctgctccg acaaactatt tatcagcagt ggcaacaaat ttttccaatg
    27601 tacttgtgat gtatcctgaa actcatctat caaaaaattt ctgtatttgt ttccggcctt
    27661 ttcataaatg aaaggtgtat cattatttcc gataatggag tttaaaattt tacccacatc
    27721 actcataagc aaaatctgat gatctttgcg ataaattttc aactgcctaa acagatattt
    27781 aagaattacg gcttgataaa tatttgcagt aacaacttca gcagataaca atcgctcaat
    27841 gttggttata taataatcaa caatttgccg tactaaagac aataattcac cattcagact
    27901 tacatcttgt ttaagaaaat cctgggcatt atctgcaaaa acatcttcac gatcaaggat
    27961 ctgccatgta cgttgaccta tagtactatc tttaggatga acctttgcag caatttttac
    28021 tataaaagaa tgaaatccac ctctaccata gtaaaaatca ataggctcta ctcctgctga
    28081 atctgctatc tccaaaaatt tctttgccat tcctgacaat ttgttccaga tagcagcgta
    28141 caaactcttt acttcattaa tcccttctgt gtcggcaaga acgtcagttt caaaattaac
    28201 tttaaggtga tctttaaaaa tttcttttgc aatgctcctc agttcacctt gcatattcca
    28261 actcttatta ctatccactc tgtacattgc aaaatcaata agccaattgg taatgccaac
    28321 atctgttccg gcagctagca aaatattttg aaccataaaa tctatggctg gttctgtatt
    28381 taaatcaatc tcatatctac ccggcaacct tgattcataa gctatggaac gggtgatgtt
    28441 cagaaaaaaa ctatctattg tactgatgga aatgtcagca taatgatgaa tagcataatt
    28501 caacaccctt gctgctgctt gttcaatatt aaatgatgtt cctaatgaag aggctaaatc
    28561 tgattttaat ttttcatttc gaccggttgc caattgccat aaagcaagga ttagtctttc
    28621 tttcatttca ccggttgcag cattggtaaa tgtaactgca agagttcctt ttaatgctgc
    28681 tggcgatttt aataccagca acaaatattc tttaacaagt gtgtatgttt tacctgaccc
    28741 tgcggacgat tgatatattt tcagatttcc caaaatgcct tttttgaatt ttcaaattta
    28801 agtatttggg atgattttct atgacgaatt cgaaatagat tgaataacat
//
LOCUS       DAOOOI010000019        28161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_300_length_28161_cov_32.680780, whole genome shotgun sequence.
ACCESSION   DAOOOI010000019 DAOOOI010000000
VERSION     DAOOOI010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 28161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28161
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_300_length_28161_cov_32.680780"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PKX59_04290"
     CDS             <1..634
                     /locus_tag="PKX59_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ90118.1"
                     /translation="EARPEKMVLFKGQLKNDDNEVPDSARIEIRNISSKQVEQIDYDA
                     YSGKYAGVLQANSEYLLTIQKDDYAFTSKLIDAAKVDSPAVTVELDLRKIKEGEAYSL
                     NDILFETDSYQITDKSKAVIQDFAGFLKSNPKLKVAIYGHTDNSGKAEDNLLLSKNRA
                     KAVYDYLIALGIEATRLSHNGFGQTKPVAANTTEKGKSLNRRTEFVVLKK"
     gene            639..1133
                     /gene="idi"
                     /locus_tag="PKX59_04295"
     CDS             639..1133
                     /gene="idi"
                     /locus_tag="PKX59_04295"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017213152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOZ90119.1"
                     /translation="MEFVILVDEQDSEVGEAEKMQTHKLGLLHRAFSVLIYNSNGEML
                     LQQRAETKYHSGGLWSNACCGHPRPGENIEDAAKRRLKEELGFNCDLKFSFKFLYKVS
                     FENSLKEHELDHVFTGSYESEVLPDKSEVQAFRWITMNNLKNEITENPDKFSVWFLKI
                     ISEL"
     gene            1218..1877
                     /locus_tag="PKX59_04300"
     CDS             1218..1877
                     /locus_tag="PKX59_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOZ90120.1"
                     /translation="MNTFNFRCRNFILSFLFFSMPVLAFAQSGQEKLVQLSGVIVTGD
                     SLSPVPYTGIMIKSSSRGTVSDYYGFFSIVAHVGDEIEFSSVGFAKTIFKVPDTLTEN
                     RYSLIQILNRDTVLLNQAVIYPWPTREQFKQAFLDLKVPDTDMDHARRNLALETMREE
                     SIRIDMDGSMNSKFVYQKQQSRLYNTGQLPVNNLLNPIAWAKFIQAWKSGEFKSKSKD
                     D"
     gene            1877..4402
                     /locus_tag="PKX59_04305"
     CDS             1877..4402
                     /locus_tag="PKX59_04305"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ90121.1"
                     /translation="MRQLIVFLSLLFCCYSLNGQDATARIHGKITGTDRKPLPGVSVG
                     IAGTSLGVISDEHGEYSITVPSNRELSIVFSSIGFEQATVKLILAEGEKKQLNRTMLP
                     SIVPLPNITIIDPRERSGTLIKIDPKAVEEIPNPSGNFETILKTIGSGVVSNNELSSE
                     YSVRGGNYDENLVYVNDVEIYRPQLVRSGLQEGLSFINPDLVSGIKFSAGGFDAFYGD
                     KMSSALDIKYKRPTEFAGKITYGLLGGGLSLEGTSKNNRFTYLIGARQKSTKYLLGTL
                     DTKGNYKPVFYDVQALLTYDVSEKTTISLLGNFSKSRYNIVPQTRTTDFGTVSQALRF
                     TVYFDGKEVDDFTTTTGAVIFSHSPKKNINLKLIGSVFNTKEEETFDVLGQYFLDELE
                     KDPASQNFGEVKLNLGVGSFLNHARNFLDGKVYSAEHKGTYNYRHNELLWGIRFQHEQ
                     FSDQIKEWNYIDSSGYSLPHPQDSVGGSLPSQPQLLLQDVISRDNSLSSNRLSGYLQH
                     NFYFSNTSNFILTPGIRFTWWSLNHVLNISPRIALAYKPRWKRDFNFRLSGGFYYQPP
                     FYKELHNPDGSLNTSLKSQQSIHVVGGTDYYFKAWGRDFKFVTEAYYKWLNDLIPYKV
                     DNVRIRYLGKNNSKGYATGIDFRINGQFVNEAESWASLSLLKTEEDIQDDVYYIRLNA
                     RGDTIRGYTIDKKAVDSIQVIPGYLPRLTDQRLTFSIFFSDYLPKFPTFKMHMTLVFG
                     TGFPVGPPGNNRYTDINRYPFYRRVDIGFSKIFIDENRPAKSRFAAFNKLKALSLSIE
                     VFNLFQVNNVVSYTWVSDVYGTKYGVPNYLTGRQLSLRLSAKF"
     gene            4545..5264
                     /locus_tag="PKX59_04310"
     CDS             4545..5264
                     /locus_tag="PKX59_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOZ90122.1"
                     /translation="MRNHSESRFLTERQSRIGEFLFTLKVVREFIKGFRTLHFVGPCV
                     AVFGSARFKEEHHYYQLGREVGANVSRLGFTVMTGGGPGVMEAANRGAKEAGGFSVGC
                     NIILPREQQPNKYLDKMVEFHHFFVRKVLMFKYSYAFVVLPGGVGTIDEMFEALTLIQ
                     TKKVSDFPVVLMGKNYWQPVIDQLKVFDEHKTALHSELDSLLITDSLDEMTEYLRVKA
                     IERFKLSKAMKFNRSKWFLEE"
     gene            5360..6364
                     /locus_tag="PKX59_04315"
     CDS             5360..6364
                     /locus_tag="PKX59_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOZ90123.1"
                     /translation="MKVFRLCFFIFISSMLQAQDIHFSQFYNSPLTLNAANTGLYEGD
                     YRFANTYRSQWSKLDPGYRTNAFSFDKTLYTGADQISGGFLFIYDRSGINRLQSIRLG
                     LSAAYHRTFGVHSLSAGFQWNYIFKSIDRSKITMPEQFNMNTGYFDPSMPTGEMLLDE
                     KRSFGDINAGLVYQVELNKHFIQAGASFFHLNKPGDTFFNSSNKLPVRTCLTVSDKIG
                     IGNDYQLHTRLLMMQQVKANEYVAGVTLVKQLNNGESKFTEVNGGLYVRTSPGNGTDA
                     VIPVIGIKYDLFDVALSYDINISTLHTATASRGGFEISLIYTALSSRLFKIKIPCERY
                     "
     gene            6351..8438
                     /locus_tag="PKX59_04320"
     CDS             6351..8438
                     /locus_tag="PKX59_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ90124.1"
                     /translation="MNAIKTRAILLLILLISSVHLYAQQNDELKKYGTAQLKRMGSYA
                     AKINDFSSSAMYYEEYCKRKPTNEKVWFLYAESLRKSKDYRKAEDAYMKAYNLNPKLN
                     ALALYHYANVQKSLGNYKKAEEYFTKFKKEYKGSDKSVYTKLIKNSGDAARFADSLMH
                     SPSKVAIEHLPEDINSSHVESSPFYINDSTIIYGSLKTDQVTFDFNPRDSSADEPFRK
                     IYVAVRDSEIWKDDGELPGFYNSNSAHTLNGVYSQDKSRFYFTRCKRNRQNKVICAIY
                     VSFLNNGIWTEPVSLGTEVNDPKYTSTQPAIGLNTQKKGEEIIYFVSDREGGKGGYDI
                     WYTSFDNRSKTYKTPVNLGTKINTSGDEVTPYVDAQTQTFYFSSDGWQGLGGLDIFKS
                     IGGMKKWTHPVNLGYPVNSNYDDLYYVLSPSNKDIGMIVSNRNVTDNGMNKGPCCDDI
                     FEFNILDAVRLNVKGFLYETDSTQKGKKLAMMPVRRARITLEMKNAEDSTFIPVSTSN
                     TDETGHFNLIVSPRQEYRITARKEGYLYESVALSTMNKTRSGDISIDIHIRSIPETPL
                     LLTNIYYEFASPLLTAKAMAALDTTLVIFMQRNVDLGVEIRSHTDAIGSDDANMTLSQ
                     KRAESVVNYLVAKGINANRLRAKGFGETMPVAPNTKPDGRDNPKGRQLNRRTEFKILG
                     KMRDGVIREETDF"
     gene            complement(8441..9025)
                     /locus_tag="PKX59_04325"
     CDS             complement(8441..9025)
                     /locus_tag="PKX59_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
                     /protein_id="HOZ90125.1"
                     /translation="MIYEFNGFRPVVDSSSFVHEQATVTGDVIIGKQVYIAPGAAIRG
                     DWGTIIIEDGCNVQENCTIHMFPGVTVILKQGAHIGHGAIVHGATIGKNSLIGMNAVI
                     MDNAVIGDGCIVGALCFVPDGMIVPDRKIVVGNPAKIIKDVSDEMLHWKTKGTELYQQ
                     LPHELTATLKPCAPLRSIEPNRKKHDAVYTTWKK"
     gene            9160..13593
                     /locus_tag="PKX59_04330"
     CDS             9160..13593
                     /locus_tag="PKX59_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90126.1"
                     /translation="MSKYFASIILVFIFLTSTLRAQTLKSFNTDPSSYLLDLKSFFEE
                     TNKKEAEKIMEEFKPVFLGKFNAQQQQTIITTSNLMLKKRMKAFPDFVTYTSVLNAFA
                     SSGQEAATFTSWNSSFEKAIAKLSVRKLGDFLEISQLLFRNNTLYESSAVAWSASNNK
                     FSFGFDSLPKVTFSGMTLRCLGKGDSSVIEGTKGVYYPNNLLFFGDGGTVNFTRAGIS
                     VSEANAVVKRYAINLKGSEYSMDSVAFTYKKYFDQELKGRYIDKLLANVNDSNATYPR
                     FYSYAANLDIKNMVKDADYKGGFSLQGSKMVGSGNRRQDASITFNLNGKPQLKLLSQG
                     FIFRTDRIVSVNAAAVIYWEKDSIYHPGVEFKYIYGDKTVTLTKNGQTAINSPYFDSY
                     HKMDLDFDQLVWKITDPLMDLKMISGGGESKLKFESVNFFSRQRFDKIQGLSEVHPLF
                     KIKQYAEEHNVKVISVPEFAEYLKLTENTVRNQILQLSSLGFISYDADADKFIVKDKV
                     MYYLNARTGKVDYDELKIESMISGLSNATINLLNFDINMRGVSHVLISDSQEVYIQPL
                     DQEMTLKKNRDIVFNGRVHAGRLDFYGKEFTFNYADFNIDLKNVDSLRFKVPADSVDE
                     RGKKRLIPIKSILQQLNGKLSIDNPTNKSGFVPNEEYPVFVCTNKSYIYYDYPEIFGG
                     VYKRDKFYYHLDPFSIDSLNTLTKEGLVFEGSMVSADIFPEWRQQLTVQRDYSLGFIT
                     NTPDAGFAAYSGKGKYRDQISLSHEGFLGKGEVDYLSSTSKSNNIIFFPDSMHAVTQS
                     FAIRKEQVGATRFPDVKNEDVFVNWRPKSDKMFVFKRSKDFALFNGEAVLDGNLVLMP
                     SGIKANGIIRYQQSQLISNNHFIKEHSYGSDSAEFKLNTDAGDLLALHTQNVKANIDL
                     DKRIGDFSSQGEKAKVEFPVNEYISYIRDFKWYIDKSELEFGGSDATADKANEFVSVN
                     RQQDSLRWDAPLATYNLKSYLLKGFKVKEILVADASIIPDLGNVVVERNAVMRPLVNS
                     KVIANTTSHYHTMLNTNITIQSRMNYSGDGNYEYMDQTKVKHKIKLTQIGVDTSKQTF
                     AIGEIPDSSRFFISPTIQYKGKLRINAPKENLYFNGFAIVNHHCNTFERNWFGFAGDI
                     DPKGVNIPIQSPANESLQKLYSGIYFGSDSTMMYPAFLSMKKHSSDAEVISAEGYLTF
                     DNNLQQYKISGTIPGTDSTKSASVITNTVILDDKRCQLIGEGRINLTQDFGQLSITNF
                     GMVNYSTVNDSIGFDAVMATDFMFNDDALKQMAEMFNSIPALQPFMDDRPAYRNALTN
                     MIGKNKAEKLLNETGLYGSARRIPSELQHTLMLSELKFYYNKERASYISVGNIGLGVI
                     GKTYVGRMVKGKVEIMRRRSGDVLNIYLEALPDMWYYFNYQRGVLQAISSDEKFNKPI
                     DEMKPEKRIASEKGGLEPYQFMLATERKKNEFLKRIKDADL"
     gene            13649..14122
                     /locus_tag="PKX59_04335"
     CDS             13649..14122
                     /locus_tag="PKX59_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOZ90127.1"
                     /translation="MQREHHYQANIIWTGNKGSGTDSYLGYARSYTVNIDNKPEIQGS
                     ADAAFHGDKTKYNPEEMLLIALASCHMLWYLHLCSEAGVVVVEYTDRATGLMTETPSG
                     NGKFESVTLNPIVVIRDQTKNEIAIELHKKANEFCFIANSVNFKVNHKPVFKLMK"
     gene            14274..15242
                     /locus_tag="PKX59_04340"
     CDS             14274..15242
                     /locus_tag="PKX59_04340"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HOZ90128.1"
                     /translation="MKNTFFTVAFILCAFFVKAQSSNVILFTENGEKFFAILNGVRQN
                     TSPETNVKVTGLNANFYKLKVIFEDTKLGEKDFNLAFNPGMETTFSIKFIAKKNSYVL
                     RPLSEVPLAQALPPAANQTVVAYSEVPAPPPAATTVTHTTTTTTTDGTPMPGDNVNVN
                     IGVNMNEAGGGINMNVSGGTTQTTTHTTTTTVTHTTTSEPTAPPIAPPPPAPVVYLPG
                     YNGPYGCPVPMAPQDFSELKQSISSKSFEDSKMTIAKQVLRNNCLFTSQVKDLMGLFT
                     FEESKLDFAKYAYDYTYDIGNYFKVNDMFTFESSIDDLNNYINSKH"
     gene            complement(15308..15754)
                     /locus_tag="PKX59_04345"
     CDS             complement(15308..15754)
                     /locus_tag="PKX59_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ90129.1"
                     /translation="MVIREAKIDDIKQIQRVRNSVKENTLSNPDLVTDKDCAEYISER
                     GKGWVCEIENQIVGFAIADLKENNIWALFLDPKFEKMGIGRHLHKTMLDWYFSQTKQT
                     VWLGTAFNTRAEQFYRKAGWTEVGTHRSKEIKFEMTYSDWIKIPNH"
     gene            complement(15839..17674)
                     /locus_tag="PKX59_04350"
     CDS             complement(15839..17674)
                     /locus_tag="PKX59_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOZ90130.1"
                     /translation="MRLIRNFLVLSIFWMLFFGIFRIIFFLFLQYNAANIQLNELPST
                     LFYGIRLDISATAYISALPLLLMLIHALTENTFIKKLFLVYNGIILFVLILLHVSNLF
                     LYRSWGVLINYRALSFLADWRQMFLSVNNFELIVGIICIILLFILTWFVFRKFTKHFF
                     DNKLAIIQSLLQFIVSAAMLVIMFRGGVQMLPVNESAAAFSENVTLNHIATNPAWHLG
                     HNLNQAKSENASYYKFYSDAVAEEHVKKLFQTPIDLMPVLNNKKPNIVIIMLESFTVD
                     VVGSMGGETGVSPTIDSLIHDGLSFKNIYSSGARTDQAFVAVLSGFPAQPNQSIMRFP
                     DKTAKLPSLPAELKKQGYSTSFYYGGDLDFSNLNTYLVNCGFEQITGKKKFKQQQLSA
                     KWGAHDEFVFEKNLNDLNQAKEPFFSVVMTLSSHEPYDIPIQSPFGKDNPNEQFKGSA
                     WYADHSLGNYLAAAKKEPWFKNTLFVIVADHGNKIPRDRSYYSHYIRHIPMVMAGDVV
                     KNIYKNKIVYSTGSHHDIPSTLLHQLGFNSDMFNWSNDLLNRKRNGFAYLCMDDAEGW
                     VTDSCTFVYDLSNQKFIYKRPYYAIADTVTAKSYLQQLYSTFSNL"
     gene            complement(17676..18416)
                     /locus_tag="PKX59_04355"
     CDS             complement(17676..18416)
                     /locus_tag="PKX59_04355"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOZ90131.1"
                     /translation="MAKIINWFCFLLLLVFCSCQKDHMFDCFKGTGNDKSEIRNITGF
                     NRIKASNNVDVKIYPGYDYQVEVMAGSKLIEGITTEVKDSMLYIRNENACNWVRSFKN
                     KFTVKVWMPDIAELTVNGSGNIDLMDTIRHNEFTFNNWGASGKVTFLFNTGAVRTNIH
                     TGPGDFVFHGYVGVHYFYNNGNGIADASNLNTEVTFTENKGANKEYVRAKQWLTAKIS
                     YVGDIYYFGNPDSVVHEGTGNGKLIKAD"
     gene            complement(18400..19353)
                     /locus_tag="PKX59_04360"
     CDS             complement(18400..19353)
                     /locus_tag="PKX59_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOZ90132.1"
                     /translation="MSELTIPVDLENPVAFFGINDSNLDLLRKKFPAFKLIVRGNELK
                     ILGDEKQIHWVAKKIIGAIEYFHHYGKFDEQVLEKLMADGQNSDTLVNPPSGNGSDVL
                     VFGPNGVVVKARTANQQKMVDSCNKNDIVFAIGPAGTGKTYTAVALAVRALKNKEIKR
                     IVLTRPAVEAGENLGFLPGDLKEKIDPYLRPLYDALFDMIPGEKLKTFIENGVIEVAP
                     LAFMRGRTLDHAFVILDEAQNTTESQLKMFLTRMGPAAKFIVTGDVTQIDLPNKQPSG
                     LIQAMKILKNIKGIDFITLDAGDVVRHRLVKDIILAYGKNH"
     gene            19527..20312
                     /locus_tag="PKX59_04365"
     CDS             19527..20312
                     /locus_tag="PKX59_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOZ90133.1"
                     /translation="MSLITLTTDWGLTDHYVAALKGELLSMLPGVNIVDISHQIPAYD
                     FTKAAFVFKNAFYFFPPQTIHLICVDKQSKKTEDTHSGWMVIQYGESIIVCRNNGFFT
                     LVSKQEPLKSVYIEGSEEVSVAHEKSFLVTLLHDLVHHKPLEEIGQPIKSFYQSSMFQ
                     PSHDANTIQGTVIHIDTYGNAITNIEKEYFENVVGAKRFEIDMPRHGDAITKIYNRYD
                     DVTPGQLVALFNASGYLEIALNQGDISLLYGLEYGDKIRIFLQ"
     gene            20309..20617
                     /locus_tag="PKX59_04370"
     CDS             20309..20617
                     /locus_tag="PKX59_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOZ90134.1"
                     /translation="MITRIVRLPVKPDEGKNFEQLFDTIKEKIAAFDGCLSVELHKDV
                     ASSDYFTYSLWQSESHLQAYLDSSFFTDVWPNAKKLLHQRANAWSLEKVLNVWKVAKF
                     "
     gene            complement(20965..22173)
                     /locus_tag="PKX59_04375"
     CDS             complement(20965..22173)
                     /locus_tag="PKX59_04375"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ90135.1"
                     /translation="MTKKYPFHYEVRVKLLAFDKKNNVRNESFIKKFDDKTPLENRAN
                     AFEEFNEYLSFLEQIGRLKKSNQGNFVITQPSFINEILDKKENSEDYFEFKKVYNQYK
                     EEISVYLVVNDLDIAKNVIDAFEFEGKVETEFEIHRVSSSMYDLEPQNLIDSLEMQEV
                     PLYEYYQINVGELKTTVYHYGLDYAESGEDEEGGAKRTILKTPYVWDTIERYNEIYDS
                     NQDTEETDINEGIDLLSIIKRGESNQVEFKPCLLYNFKTQAAGIGVKYLIAKAICGFL
                     NSNGGVLFIGVNDNGNIQGLENYDYSLFHGENEKDKLLLEFDSLLAYFFDLSIKPLVD
                     LSIKKVEGKDILVIVITESYKPIFLKNKKNEFIEKELYIRMNASTRQIVNIEEMVEYV
                     FNKQWKKPSR"
     gene            complement(22175..22723)
                     /locus_tag="PKX59_04380"
     CDS             complement(22175..22723)
                     /locus_tag="PKX59_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90136.1"
                     /translation="MQESTLIPFLRKNLDILFVGLNPAKGSSENGHYLSVNQAFGNQL
                     YESILLTKLVDKSNADELIFGANKYKYQNWNFGITDLITEIAESNSAKIKPKKADLIR
                     LKNVINNYKPKTTVLLHGKVLKKFISHLGYTVPESNTGKLGRLIENCNTTFYNIAFPH
                     GNSITSESKIEKYTELKEFLNL"
     gene            22841..22978
                     /locus_tag="PKX59_04385"
     CDS             22841..22978
                     /locus_tag="PKX59_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90137.1"
                     /translation="MPTIPGIDRHQIIFTDLERQISKDNVIRFIDAFVDKMDLKQLDI
                     L"
     gene            23109..23246
                     /locus_tag="PKX59_04390"
     CDS             23109..23246
                     /locus_tag="PKX59_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90138.1"
                     /translation="MAFRVEYRIVFYRKSLLSQPDGSGLNEVVDFEAQNFQFTTNVDV
                     R"
     gene            complement(23306..23740)
                     /locus_tag="PKX59_04395"
     CDS             complement(23306..23740)
                     /locus_tag="PKX59_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOZ90139.1"
                     /translation="MDSLFEIHRHLFQDIYAWAGKKRIVEISKDGKQFFPTSHFDNAF
                     RYIDLLIADFKKIPKDNKKMLSEKLTEILDNVNYLHPFRDGNGRTQREFLRLLALEKD
                     LTLNLNPPDNLSVYERYMKGTIDSDVKTLTKLIFELIDTKDK"
     gene            complement(23765..23914)
                     /locus_tag="PKX59_04400"
     CDS             complement(23765..23914)
                     /locus_tag="PKX59_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90140.1"
                     /translation="MSNSYKYIDPDFTYTDPKTGLLRNLQDITDADVLLFVESGAVTK
                     RLQEL"
     gene            complement(23907..24128)
                     /locus_tag="PKX59_04405"
     CDS             complement(23907..24128)
                     /locus_tag="PKX59_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010607961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="HOZ90141.1"
                     /translation="MFNTIEIDRNNLTIMGVKFSDLKTLESTANALGNSMFEGFKPTP
                     KGIEIIRDYVTGKISLTELVAFAKQKAYV"
     gene            complement(24477..25829)
                     /locus_tag="PKX59_04410"
     CDS             complement(24477..25829)
                     /locus_tag="PKX59_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOZ90142.1"
                     /translation="MKRTLFEYDKWVKVENSTALFHSLKSIWRQRNFTDLTDELEENE
                     TDKNYQPFLRFSNNEIRANNYVGFIQTANDLVEIYPKVFRNCPDKDKNDMLRHIFFWF
                     NYCSKWKFPFSQAQLDRFSIDEFPELIINLIANQFLETVSNQPLTMYQPVEEAMQTPR
                     GSINFKRYIANSLVSGNFQNIECDYEPFLFDNKVNRVIKYCSRLLMNQTRFAENIQML
                     QEVVFILDEVEDIPCNSYEVENITLNSFFEDYNKVLDSCKLILNQQLYSSNTYDLSQW
                     CLLFPMEYIFEDFAAGFLADKFSNDWKVEYQKSNEYLSSNPKAFNMQHDIFLTSKSAD
                     RRKVIIDTKYKLRSFDFKNDIKKGIAQADLYQMTSYAFRRGCTDIFLLYPNITDDINE
                     PDIFEINSGFSIDDKITITAMEIPFWTTENLDTMTLEIKLFNTLKTQLYKLKTESPNR
                     "
     gene            complement(25830..27827)
                     /locus_tag="PKX59_04415"
     CDS             complement(25830..27827)
                     /locus_tag="PKX59_04415"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ90143.1"
                     /translation="MGYEIKAKQESELTGILWKLGCNWGKGKPSFYNLIKDKGITIGV
                     DDKKYKIGDLIIVCEGHTVKAIAKIEETPISVTTNRELEKPFNDFQIEYEDWINFAKS
                     EWYELAEDEIFQYQLQQGIVRVQSREVYQKALQLWNNRHNKRMNYKEQYTTWLNSTNK
                     EGSKKAGSYIRAIEILSEITHKEIFEEDKLTVLNALYQDLLNEQEKEDGKYYYEQAPS
                     YGASRFYSAAIKSYSDFIAQLPKTQIAKTLLITNMPLNSILYGPPGTGKTYNSIDKAV
                     AIATGSSTDHVSNKTQFDELRKQGQIEFVTFHQNYSYEDFMVGIRPNVSETTSELSFK
                     KHYGIFYEIAKRARENYENSLKDEQTISKENWVNEKFEEFKEYVEAEIEENGKFAIKN
                     NVSIYSVEDEVFIYTGEKENGELWKNQRIGMHYDALINIYLADVQNRQDIKKLENVPS
                     LEKQHATYSFELIEKFRKFLKEKGYKFQSTTVQKEPLKNFVLIIDEINRANISKVFGE
                     LITLLEDDKRLGKPNELKISLPNGEKEFGVPPNLYLIGTMNTADKSIALIDIALRRRF
                     EFIGYYPDYENEKLKSEAKTLLQKINEEIYKLKNSADYLIGHAYFMKDEAIETTLRNK
                     VIPLLMEYFSGKTKIVSDIFNETTWKVEYDTTQFNWTISLR"
BASE COUNT         8838 a         4747 c         5124 g         9452 t
ORIGIN      
        1 ggaagcaagg cccgagaaga tggttttatt caaaggccaa cttaaaaatg atgacaatga
       61 agtgcctgac agcgcccgta tagaaattcg gaatatttca tccaaacagg ttgagcagat
      121 agattacgat gcatacagtg gaaaatatgc aggtgtattg caagctaatt ctgagtattt
      181 gttaactatt caaaaagatg attatgcatt tacttcaaaa cttattgatg ctgcaaaagt
      241 tgattcgcct gcagttactg ttgagttaga tctgagaaaa ataaaagaag gcgaagcata
      301 tagtttaaat gatatacttt ttgaaacaga ttcttatcag attacagata aatcaaaagc
      361 agtaattcag gattttgcag gctttcttaa aagtaatcca aagttaaagg ttgcaattta
      421 tggtcatact gataattcag gaaaagcaga agataatttg ttgttgtcaa aaaacagggc
      481 gaaggctgtt tatgattatt tgattgcttt aggcattgaa gcaacacgac tttcgcataa
      541 tggattcgga caaacaaaac cggttgctgc aaatacgaca gaaaaaggta agtcactgaa
      601 cagacgcaca gagtttgtag tgttaaaaaa atagttttat ggagtttgtt attttggttg
      661 atgaacagga tagcgaagtt ggtgaggcag aaaaaatgca gactcataaa cttggattat
      721 tacacagagc tttttcagta ttaatttaca attccaatgg agaaatgctt ttgcaacaaa
      781 gagcagaaac aaaataccat agtggaggac tttggtcaaa tgcttgttgt ggtcatccac
      841 gtcccggtga aaatatagag gatgccgcaa agagaagact taaagaggag ttgggcttta
      901 attgtgatct gaagtttagc tttaaatttc tttataaagt ttcttttgaa aatagtctta
      961 aagagcatga actggatcat gttttcacag ggagttatga aagtgaagta cttcctgata
     1021 aaagtgaagt tcaagccttc cggtggataa caatgaataa tctgaagaat gaaattacag
     1081 aaaatcctga taaattttct gtttggtttc ttaaaattat ttcggagttg taattccttg
     1141 ttatttagct gataatattg aaataaatta ccgaatcata cgttttgctt gaatgcaaat
     1201 ccttattttt ggttcctatg aatacattca atttcagatg caggaatttt atcctgtctt
     1261 ttttattttt cagtatgcct gttctggcat ttgcgcagtc tggtcaggaa aaacttgttc
     1321 aattaagtgg tgtaatagtt acaggggata gtttaagtcc ggtaccctac acaggcatta
     1381 tgattaagtc gagcagtaga ggaacagtgt ctgattatta tggttttttt tcaattgtag
     1441 cacacgttgg tgatgagatt gagttttctt ctgtcggatt tgcaaaaact atttttaaag
     1501 tacccgatac gctcacagaa aaccgttatt ctttgattca gattcttaac cgtgatactg
     1561 ttttacttaa tcaggctgta atttatccat ggcctactcg cgaacaattt aaacaggctt
     1621 ttctcgattt aaaagttcct gatacagaca tggatcatgc acgaagaaat cttgctcttg
     1681 aaaccatgcg cgaagagagc ataagaattg atatggatgg aagcatgaac agtaaatttg
     1741 tatatcagaa acaacaatca aggttataca atacaggcca attgccggta aacaatttgc
     1801 taaatcctat tgcatgggca aagtttattc aggcctggaa aagtggtgag tttaagtcaa
     1861 agtctaaaga cgattaatga ggcaacttat cgtttttctt tctttgttat tttgttgtta
     1921 ttctcttaac ggccaagatg caactgctcg tattcatggc aaaataacag gcacagaccg
     1981 taaacctttg cctggtgtta gtgttggcat tgcaggtact tcattaggtg taatttctga
     2041 tgaacatggt gaatattcaa ttactgttcc atctaatcgt gagctgagta ttgttttttc
     2101 atcaatcggt tttgagcagg caacggtaaa attaattctt gcagaaggtg aaaagaagca
     2161 attaaacaga accatgttac catcaatagt gcctctgccc aatatcacta tcatagaccc
     2221 tcgtgaaaga agtggcacat taataaaaat tgatccaaaa gctgttgagg aaatcccaaa
     2281 tccatcaggt aatttcgaga ccattttaaa aacaattgga agtggcgttg tttcaaacaa
     2341 cgaacttagt tccgaatatt ctgtgcgtgg cggaaactac gatgaaaatc tggtttatgt
     2401 aaatgatgtt gaaatttaca gaccacaatt agttcgctca ggactacagg aaggtttaag
     2461 ttttatcaat cctgatcttg tttcaggaat taaattttca gccggtggtt ttgatgcttt
     2521 ttatggagat aaaatgtctt cagcattgga tataaaatac aaacgaccaa cagaatttgc
     2581 gggaaaaatt acttatggtt tgcttggtgg aggtctgagc cttgaaggta caagtaaaaa
     2641 caaccgtttt acatatttga taggagcacg acaaaaatca acgaaatatt tattgggtac
     2701 acttgataca aaaggaaact acaaacctgt tttttacgat gtgcaggcat tgttgaccta
     2761 tgatgtgagt gaaaaaacta caatttcttt attaggtaat ttttcaaaaa gcagatacaa
     2821 tattgttcct caaacacgaa caacagattt cggtacagtg agtcaggcat tgcgattcac
     2881 ggtttatttt gacggaaaag aagttgatga ttttactact accacaggag ccgtaatttt
     2941 cagtcatagt ccgaagaaaa atataaatct caagctcata ggctctgtgt ttaacacaaa
     3001 agaagaagaa acttttgatg ttcttggtca gtattttctg gatgaacttg aaaaagatcc
     3061 tgcctcacaa aattttggtg aggtaaaact caacctcggt gtaggttctt ttttgaatca
     3121 tgcacgcaat tttcttgacg ggaaagttta ttcagcagaa cataaaggta catacaatta
     3181 tcgccataat gaattgttgt ggggtattcg gtttcagcac gaacagttca gcgatcagat
     3241 taaagaatgg aattatattg actcatcagg ttattcatta ccacatccgc aggatagtgt
     3301 aggagggagt ctcccatcgc agccgcaatt attattgcaa gatgtcatca gtcgtgataa
     3361 cagcctttca agtaacagat taagcggcta tctgcagcat aatttctatt tcagtaacac
     3421 atcaaatttt attctcacac ccggtattcg ttttacgtgg tggagtttga atcatgtttt
     3481 aaacatcagt ccacgtatag cacttgccta taaaccaaga tggaaacgcg attttaactt
     3541 ccgtctttca ggagggtttt attatcaacc gccattctac aaggagttgc acaatcctga
     3601 cggaagttta aatacatctt tgaaatcaca acaatctatt catgttgttg gcggaacaga
     3661 ctattatttc aaagcgtggg gtcgcgattt taaatttgtt acagaagctt attacaaatg
     3721 gttgaatgac cttattcctt acaaagttga taatgttcga atacggtatt tgggtaaaaa
     3781 taattccaaa ggttatgcaa caggaattga ttttcgtatc aatggccagt ttgttaatga
     3841 agcagagtca tgggcaagtt tatcactttt aaaaacagaa gaagatattc aggatgatgt
     3901 ttattatatc agattgaatg cacgtggcga taccatcaga ggatacacca ttgacaaaaa
     3961 ggctgttgac agtattcagg ttatacccgg atatttacca cgattaaccg atcagcgact
     4021 tacattcagc atattttttt ctgactatct tccaaaattt ccaacattta aaatgcacat
     4081 gacccttgtt ttcggaacag gatttcctgt aggcccgccc ggaaataatc gttatacaga
     4141 cattaaccgt tatccttttt acagacgtgt tgatattggg ttcagtaaaa tatttattga
     4201 cgaaaacaga ccggcaaaaa gtcgttttgc tgcgttcaat aaattgaaag cgctttcact
     4261 ttcaattgaa gtgtttaatc tttttcaggt taacaatgtt gtttcctata cttgggtgag
     4321 tgatgtttat ggtactaaat atggcgtacc taattatctt accggaagac aattaagttt
     4381 acgactcagc gctaagttct gattacatac gtcacaagat tattagcttt tcacagtaaa
     4441 aataattttt tgattcctca tctttaaatc ataagctgat ttgtttatat actcattgcg
     4501 ttttgaagaa tcttttaaat ttgaattttt aaatttttca gcttatgcgt aatcattccg
     4561 aaagcaggtt ccttaccgaa cgacagtcgc gaattggcga atttctattt actctcaaag
     4621 tagtaagaga atttataaaa ggattcagaa cattgcattt tgttggacct tgtgttgcag
     4681 tgtttggctc agcacgattt aaagaagagc atcactatta tcagttaggc cgtgaagtag
     4741 gggcaaatgt cagcagattg ggatttacag tgatgaccgg tggcggtccc ggtgttatgg
     4801 aagcagctaa cagaggggca aaagaagcag gaggtttttc tgtgggttgc aacatcattc
     4861 ttcctcgcga acagcaaccc aataagtatc ttgacaaaat ggttgaattt catcattttt
     4921 ttgtcagaaa agttttgatg ttcaaatact cctatgcttt tgtggttttg cctggaggtg
     4981 ttggcacaat tgacgaaatg tttgaggctt taaccttgat tcaaacaaaa aaagtaagtg
     5041 atttccctgt agtacttatg ggtaaaaatt actggcagcc tgtaattgat cagctcaaag
     5101 tatttgatga gcataagaca gccttgcact ctgagttaga ttctttgctg attacagata
     5161 gtttagatga aatgacagaa tacctgcgcg tgaaggctat tgagagattt aagttgtcta
     5221 aagccatgaa attcaaccgc tcgaaatggt ttcttgaaga atgagcctga ctgttggcaa
     5281 ttattcaata aaaactttcg caattcattt ctatcttata tttttatcct gttatttttc
     5341 agagtattta caggactaaa tgaaagtatt ccgcctttgc ttttttattt ttatttcttc
     5401 aatgctgcaa gcgcaggata ttcatttttc acaattctat aattctcctc tcacacttaa
     5461 cgctgcaaat acaggattgt atgaaggcga ttatcgtttt gcaaatactt atagaagtca
     5521 gtggtcgaaa cttgacccgg gttacagaac caatgctttt tcatttgaca aaacacttta
     5581 taccggagca gatcagatta gcggaggctt tttgttcatt tacgaccgtt cgggaattaa
     5641 tcgtttgcaa agtattcgct taggactatc tgctgcttat catcgcacgt ttggagtaca
     5701 cagtttaagt gcagggtttc agtggaatta tatttttaaa tctattgaca gaagtaaaat
     5761 taccatgcct gagcagttta atatgaacac cggttatttt gatccatcaa tgcctaccgg
     5821 tgaaatgttg cttgacgaga aacgttcttt cggtgatatt aatgcagggt tagtttatca
     5881 ggttgaatta aataaacatt ttattcaggc aggtgcttca ttttttcatc ttaacaaacc
     5941 gggtgatact ttctttaaca gcagtaataa attacctgtt cggacatgtt taaccgtttc
     6001 tgataagatt ggaattggta atgattatca gttacacact cgattattga tgatgcaaca
     6061 ggtaaaagcc aatgagtatg tagccggagt tacacttgta aaacaactta ataacggaga
     6121 atcaaagttt acagaagtta atggtggttt atatgtacgt acatcacccg gtaacggcac
     6181 tgacgcagta atacctgtaa ttggcattaa atacgatttg tttgatgtgg cactgagtta
     6241 tgacatcaat atttcaactc tccatacagc aactgcaagc cggggtggat ttgaaatatc
     6301 tcttatttac actgcattaa gttcacgttt gtttaaaata aaaataccct gtgaacgcta
     6361 ttaaaacaag agctatcctg ctgctgatat tgttaatcag ttctgttcat ttatatgcac
     6421 agcagaatga tgaattaaaa aaatatggca cagcacaatt aaaacgaatg ggtagttatg
     6481 ctgcaaagat taacgatttc agcagttctg caatgtacta tgaagagtat tgtaagcgca
     6541 aacctactaa tgagaaggtt tggtttcttt atgctgaaag tcttcgtaaa tccaaagatt
     6601 accgaaaagc tgaagatgct tacatgaaag catacaatct gaatccaaaa ctaaatgctt
     6661 tagcgttgta tcattatgcc aatgttcaaa aatctcttgg taattataaa aaggctgaag
     6721 agtattttac aaaatttaaa aaagaatata aaggctctga taaaagcgtt tataccaaat
     6781 taattaaaaa tagtggtgat gctgccaggt ttgcagatag tcttatgcat agcccttcaa
     6841 aagttgctat tgaacatttg ccagaagata tcaattcatc acatgtagaa tcatcgccat
     6901 tttatattaa cgattcaaca attatttatg gttctctgaa aacagatcag gtaacttttg
     6961 attttaatcc aagagattcg agtgctgatg agcctttccg aaaaatttat gttgccgtgc
     7021 gtgatagtga gatatggaag gatgatggtg aactgcccgg attttataat tcaaacagtg
     7081 ctcatacact caatggtgtt tattcgcagg ataagagtag attttatttt acgcgatgca
     7141 aaagaaatcg tcagaacaaa gttatatgtg ccatctatgt aagcttcctg aacaatggaa
     7201 tatggacaga acctgtttct ttaggtactg aagtaaacga tccaaaatat acttcaacac
     7261 aacctgccat tgggcttaac acgcagaaaa aaggagagga gataatttat tttgtcagtg
     7321 accgcgaagg tggaaaagga ggctatgata tttggtacac ttcttttgat aacagaagta
     7381 aaacttacaa aacgccagtc aatcttggaa ctaaaattaa tacttcaggt gatgaagtaa
     7441 caccctatgt tgatgcacaa acacaaacat tttatttcag ctctgatgga tggcagggat
     7501 taggaggatt agatattttt aagtcaattg gtggaatgaa aaagtggact catcctgtca
     7561 atttgggtta tcctgttaat tccaattatg atgatttgta ttatgtgcta tcaccaagta
     7621 ataaggacat tggcatgata gtatcgaaca gaaatgtaac tgataatggc atgaataaag
     7681 gcccttgttg tgatgatatt tttgagttta atattttaga tgctgtaaga ttaaatgtta
     7741 aaggcttttt atacgaaaca gattcaacac aaaaaggaaa gaagttggca atgatgcctg
     7801 ttcgaagagc acgtattact ttggaaatga aaaatgctga agattctact tttattcctg
     7861 tcagcaccag taatacagat gaaacaggtc acttcaacct tattgtttct cctagacagg
     7921 aataccgtat cacagcacgc aaggaaggtt atctttatga atcagtagcg ttgagcacta
     7981 tgaataaaac acgttccggt gatatttcaa ttgatataca cattcgttca attcctgaaa
     8041 caccattact gcttacaaac atttattacg aatttgcaag ccctttgcta actgcaaaag
     8101 ctatggctgc attagataca accttggtga tttttatgca aagaaatgtt gaccttggtg
     8161 ttgaaataag gtcgcataca gatgccattg gctctgatga tgccaacatg actttgtcgc
     8221 aaaagcgggc agaaagtgtg gtgaattatt tagtagcaaa aggaattaat gccaacaggt
     8281 tgcgtgcaaa aggatttggt gaaacaatgc ctgtagcacc aaacacaaaa cctgacggaa
     8341 gagataatcc caaaggcagg cagttgaaca ggagaacaga atttaaaatt ctgggtaaaa
     8401 tgcgtgatgg tgtaatccgc gaagaaaccg atttctgatt ttattttttc caggtagtat
     8461 aaaccgcatc gtgcttttta cgattgggtt ctatacttcg caaaggcgca cagggtttta
     8521 atgtagctgt taattcatgc ggtaattgtt ggtaaagttc agtccctttt gttttccaat
     8581 gaagcatttc atcgcttaca tcttttataa tttttgccgg attgccaaca acgattttcc
     8641 tgtcaggaac aatcatgcca tcaggcacaa aacataaagc gccaactata caaccatcgc
     8701 caattacagc attgtccatt attacagcat tcattcctat caaagaattt ttgccaatgg
     8761 tggcaccatg aactatggca ccatgaccaa tgtgtgcacc ttgttttaaa ataactgtaa
     8821 caccgggaaa catgtgaatg gtgcagttct cctgaacatt gcagccatct tcaataatga
     8881 ttgtgcccca atcgccacga atggcagcac ccggagcaat ataaacttgt ttaccaataa
     8941 ttacatcacc tgtaactgtt gcctgttcat gaacaaaact actgctgtcc actacaggtc
     9001 tgaatccgtt aaattcgtaa atcatttgaa taactgatgt ttcttgtaac gatgcaaatg
     9061 taaagctaat tgatgtgcca aaactttaac aattgttggt aactatctgt aagtattaat
     9121 ctgtcagaag tttatttttg tgctaaaacc atcacattca tgtcaaaata ttttgcttca
     9181 atcattcttg tatttatttt tctgacttca acattaaggg ctcaaacact gaagagtttt
     9241 aataccgacc cttcaagtta tctgcttgat ttgaaatcat tttttgaaga gacaaataag
     9301 aaggaagcag agaaaattat ggaggaattc aagcctgttt ttcttggtaa gtttaatgca
     9361 caacaacaac agactattat taccacaagt aatcttatgt tgaagaagcg catgaaggct
     9421 tttcctgatt ttgttactta tacatcggtg ttgaatgctt ttgcttccag cggacaagag
     9481 gcagctacat ttacatcatg gaattcttct tttgaaaaag caatagcaaa acttagtgta
     9541 aggaaattag gtgactttct tgaaatatct caattgcttt ttcgtaacaa caccttgtat
     9601 gaatcaagcg ctgttgcatg gagtgcaagc aataacaaat tttcatttgg tttcgattct
     9661 ttaccgaaag ttactttttc cggaatgact ttgcgatgtt tgggcaaggg tgatagctct
     9721 gtaatagaag gtacaaaagg agtttattat cccaacaacc ttttattttt tggcgatggg
     9781 gggacagtaa attttactcg tgcaggaata agtgtttcag aagcaaatgc agttgtaaaa
     9841 cgctatgcta tcaatttaaa aggttcagaa tactcaatgg attctgttgc ttttacatat
     9901 aagaaatatt ttgatcagga gctgaaaggc cgttacattg ataaattgtt ggcaaacgta
     9961 aatgattcca atgctactta tccgaggttt tattcttatg ctgctaatct cgacataaag
    10021 aatatggtta aagatgccga ttacaaaggt ggattttctt tgcaaggaag taaaatggta
    10081 ggctcaggca acagacgaca ggatgcatca ataactttta atctgaatgg taaacctcag
    10141 ttgaaattgc tttcgcaagg atttattttt cgtacagaca ggattgtatc tgtaaatgca
    10201 gctgctgtaa tttattggga gaaagattca atttatcatc ccggagttga gtttaaatat
    10261 atttatggtg ataaaactgt aacgctgacc aaaaacggtc aaactgccat caatagcccg
    10321 tattttgaca gctatcataa aatggattta gattttgatc agttggtatg gaagattaca
    10381 gacccattga tggacctaaa aatgatttca ggtggaggag agagtaagct gaaatttgag
    10441 tctgtaaatt tcttttcacg acagcgtttt gataaaattc agggactgtc tgaagtacat
    10501 ccgcttttca aaataaagca atatgcagag gagcataatg taaaagttat cagtgtgccg
    10561 gagtttgcgg aatatttaaa actcactgaa aacactgtgc gcaatcaaat tctgcagttg
    10621 tcatcattag gatttatttc ttatgatgcc gatgccgata agtttattgt taaagacaag
    10681 gtgatgtact acctcaatgc acgcaccggt aaggttgatt atgatgagtt gaaaattgaa
    10741 tctatgattt caggattatc taatgcaacc atcaacttgt tgaattttga tattaacatg
    10801 cgtggtgtaa gccatgtatt aatcagcgat tcgcaggagg tatatattca acctttggat
    10861 caggaaatga ctctgaaaaa aaatcgtgac attgttttca atggacgtgt tcatgcagga
    10921 agacttgatt tttatggtaa agagtttact tttaattatg ccgattttaa tattgattta
    10981 aagaatgtgg actcactgcg gtttaaagtg cctgctgact ctgttgatga aagaggaaaa
    11041 aaacgtttga ttccaataaa aagcattttg cagcagttga atggaaaact ttctattgat
    11101 aacccaacga ataagtcagg gtttgtacct aatgaggaat atcccgtttt tgtatgtaca
    11161 aacaagtctt atatttacta tgattatcct gaaatattcg gtggtgttta taagcgtgat
    11221 aagttttatt atcatctcga cccattcagt attgacagcc ttaacacact taccaaagaa
    11281 ggacttgtat ttgaaggaag tatggtgtct gcagatattt ttcctgaatg gcgacagcaa
    11341 cttacagtgc agcgagatta ttctttagga tttattacca acacacctga tgccggtttt
    11401 gcagcttata gcggaaaagg aaaatatcgg gatcaaatca gcttgagtca tgaaggtttc
    11461 cttggaaaag gagaagttga ttacctgagc agcacttcaa aatcaaacaa tattatattc
    11521 tttcctgaca gtatgcatgc agttacacaa agttttgcaa taagaaaaga gcaagttggt
    11581 gctacccgtt ttcctgatgt taagaatgaa gatgtgtttg ttaactggcg acctaagagt
    11641 gataaaatgt ttgtatttaa gagaagcaaa gattttgcat tgtttaatgg tgaggcagtt
    11701 ctcgatggaa acttagtgct gatgccttcc ggaattaagg ctaatggtat aattcgttac
    11761 caacaatctc agttaatcag caacaatcat tttattaaag aacatagcta tggttccgat
    11821 tctgcagaat ttaaactcaa tacagatgca ggagatttgc tggcattgca tacacagaat
    11881 gtaaaagcca atattgatct tgataaacgt attggggatt tcagcagtca gggtgaaaaa
    11941 gctaaggttg agtttcctgt taatgaatat atttcttaca tcagagattt caaatggtat
    12001 atagataaat cagaacttga atttggcggc tctgatgcaa ctgccgacaa agcaaatgag
    12061 tttgtgtctg taaacagaca gcaggattct ttacgatggg atgcaccttt agcaacttat
    12121 aacttaaaat cgtacttgct aaaaggattt aaggtgaaag aaattttagt agcagatgct
    12181 tcaataattc cggatttagg gaatgttgtt gtagagcgta atgcagtaat gcgaccattg
    12241 gttaacagta aggttattgc taatactact tcgcactatc acactatgct aaatacaaat
    12301 attactattc agagtagaat gaattattca ggagatggca attatgaata tatggatcag
    12361 acaaaggtta aacataaaat taaacttaca cagattggag tggacacttc aaaacagact
    12421 tttgccattg gagaaattcc agattcatca cgttttttta ttagtcctac aattcagtat
    12481 aaaggaaagt taaggattaa tgcaccaaaa gaaaatctct acttcaatgg atttgcaatc
    12541 gttaatcatc actgtaatac ttttgaacgc aattggtttg gctttgcagg cgacattgac
    12601 ccaaaagggg taaacattcc tattcaaagt ccggctaacg aaagtttgca gaaactttat
    12661 tcaggaattt attttggcag cgacagcacc atgatgtatc ctgcattctt atcaatgaaa
    12721 aaacatagtt cagatgcaga agttatttct gcagaaggct atctgacttt tgataataat
    12781 ttgcagcagt ataaaatttc cggtaccatt cccggtaccg attcaaccaa gtcagcaagt
    12841 gttattacta atacagtaat tcttgatgac aagagatgtc agttgattgg tgagggccga
    12901 ataaacctga ctcaggattt tggtcagctt tcaatcacaa attttggtat ggtcaactat
    12961 tcaacagtta atgactctat aggctttgat gcagttatgg caactgactt tatgttcaat
    13021 gatgatgcat tgaaacaaat ggcagagatg tttaattcta ttcctgcatt gcaacctttt
    13081 atggacgata gaccggctta tagaaatgca ttgactaaca tgattggaaa aaataaagcg
    13141 gaaaagttat tgaatgaaac agggctgtac ggttcggcac gaagaattcc ttctgaattg
    13201 cagcatacgc ttatgttgag tgagcttaaa ttttattata ataaagaacg tgcatcttat
    13261 atttctgtag gaaatatcgg tcttggtgtc ataggaaaga cctatgtagg aagaatggta
    13321 aaaggcaaag ttgagattat gcgcagacgt agtggagatg tattgaatat ttatctggaa
    13381 gcattgcctg atatgtggta ctattttaat tatcagcgtg gtgtattaca agcgatttct
    13441 tctgatgaaa aattcaataa gccaattgat gaaatgaaac cagaaaagcg catagcatcg
    13501 gagaaaggag gtcttgaacc ctatcagttt atgcttgcca ccgagaggaa aaagaatgag
    13561 ttcctgaaac gaattaagga tgcagatttg taagatgtaa attttagatg ggttgaattt
    13621 attaaaacca ataattagaa attatttaat gcaaagagaa catcattatc aggcaaatat
    13681 tatttggacg ggcaacaaag gttcaggtac agatagttat ttaggttacg caagaagtta
    13741 tactgttaac attgataata aacccgaaat tcaaggttca gcagacgctg catttcatgg
    13801 agataaaaca aaatataatc ctgaggaaat gttgttgata gcattggctt cgtgtcacat
    13861 gttatggtat ttgcatttat gttctgaagc aggagtggta gttgtagaat atacggatag
    13921 agcaacaggt ctaatgacag aaacaccatc aggcaatgga aaatttgaat ctgtaacatt
    13981 aaacccaatc gtagtgatta gagatcagac aaaaaatgaa attgccattg aattacataa
    14041 gaaagcaaat gagttttgtt tcatagcaaa ttcagtaaac tttaaagtta atcacaagcc
    14101 tgttttcaaa ttgatgaaat gagcgtattt taacaggaaa agcttttttt gaaaaagcaa
    14161 aattcctctt gcggcaacat gaaaatggaa acggctttat gattttttca tactttagct
    14221 ccgttttggt atcattatag ttagcaaaca atttaaataa aaatcttatt caaatgaaaa
    14281 acacattctt tacagtggca tttattcttt gtgctttttt tgtgaaagct caatcctcaa
    14341 atgttattct attcaccgag aatggtgaaa agttttttgc tatcctaaat ggagttcggc
    14401 agaatacaag tcccgaaacc aatgttaaag taacagggct taatgcaaat ttctacaaac
    14461 taaaagtgat ctttgaagac actaagttgg gagagaagga ttttaatctt gccttcaatc
    14521 cgggaatgga aaccactttc agtattaagt ttattgcaaa aaagaacagt tatgttttgc
    14581 gcccattgag cgaagtgcct ttagcacaag cattgcctcc tgcagctaac caaaccgttg
    14641 ttgcttatag tgaagtacct gcgccaccac cggcagctac aacggttact catactacaa
    14701 caactacaac aaccgatgga acacccatgc ccggtgacaa tgtgaatgta aacattggtg
    14761 taaatatgaa tgaagcaggt ggcggaataa atatgaatgt ttccggagga acaacacaaa
    14821 ctactacaca tactaccaca acaacagtta cgcataccac tacatcagaa ccaactgcac
    14881 cacctatagc acctccaccc cctgcaccgg ttgtttattt gccgggatat aatggccctt
    14941 atggatgtcc tgttccgatg gctcctcagg atttttctga gcttaaacaa tccatatctt
    15001 caaaatcttt tgaagacagc aagatgacta ttgcaaagca agtgcttcgt aataactgcc
    15061 tttttacctc tcaggtgaaa gatttgatgg gattgttcac ttttgaagag tccaaactgg
    15121 actttgcaaa gtatgcttat gattacacct atgatattgg aaattatttt aaagttaacg
    15181 atatgtttac attcgagtcg tcaattgatg atttgaataa ttacatcaat agtaaacatt
    15241 agaaaatatt ttttttaaag atagccggga atatttgtta cccggctttt tttattaaga
    15301 aaacaattta atggttgggg atttttatcc aatcactata tgtcatttca aacttaattt
    15361 cttttgacct atgagttccg acctctgtcc agcctgcttt tcggtaaaat tgttctgctc
    15421 ttgtattaaa ggcagttcct aaccagacgg tttgctttgt ttgggaaaaa taccagtcta
    15481 acattgtctt atgcaaatgt cgcccgatac ccattttctc aaattttgga tctaaaaaca
    15541 gcgcccaaat gttattctct ttcaagtcag cgattgcaaa acctactatt tgattttcaa
    15601 tttcgcaaac ccaaccttta cctctttcgg aaatatattc cgcgcaatct ttgtccgtca
    15661 ctaaatcggg attagataaa gtgttttctt ttacagaatt tctgactctt tgaatctgtt
    15721 ttatgtcgtc aattttagct tccctgatta ccatttattt attatcaggt tgaaagtttt
    15781 tttcatatta attagtaaat gttgtttctg tttgaattgt tgtggtttga gaactgactt
    15841 aaagattact gaatgtacta tacaactgtt gcaaataact ttttgcagta acggtgtcgg
    15901 caattgcgta atatggtctt ttgtaaataa atttttgatt gctcaaatca tagacaaatg
    15961 tacaactgtc tgttacccat ccttcggcat catccatgca taaataagca aagccatttc
    16021 gttttcggtt aagtaaatcg ttgctccaat taaacatatc actattaaac cctaactgat
    16081 gtaataaagt ggaagggatg tcgtgatgcg agcctgtgct gtaaacaatt ttatttttat
    16141 aaatattttt tacaacatca cctgccatta ccattggaat gtgtctgatg taatgactgt
    16201 agtatgacct gtcacgaggt attttgttgc catggtcagc aactataaca aataatgtat
    16261 ttttaaacca aggttctttt tttgcagcgg caagataatt gcccaaactg tgatctgcat
    16321 accatgctga acccttaaac tgctcgtttg ggttatcttt cccaaaaggc gactgaatag
    16381 gaatgtcgta tggctcgtga gaacttaatg tcatcacaac agaaaagaaa ggctcttttg
    16441 cttgatttaa atcgttcagg tttttttcaa aaacaaactc atcatgtgct ccccatttgg
    16501 cgctcaattg ctgttgttta aatttctttt tacctgtaat ttgctcaaag ccacagttga
    16561 ctaaataggt gttcagattt gaaaagtcaa gatcgcctcc ataataaaat gaagtgctgt
    16621 aaccttgctt tttcaactca gcaggcaagg agggaagctt agcagtctta tccggaaaac
    16681 gcatgatgga ttgattaggc tgtgccggaa atccactcaa tactgccaca aatgcctgat
    16741 cggtgcgtgc accactactg taaatatttt taaatgataa tccatcgtga attaatgaat
    16801 ctattgtggg agaaacaccg gtttcaccac ccatagaacc tacaacatca actgtaaaac
    16861 tctccaacat gatgatgaca atgttgggtt ttttattgtt cagtacaggc attaaatcaa
    16921 ttggtgtttg aaataatttc tttacatgtt cttcagcaac tgcatcacta taaaatttgt
    16981 aatatgaagc attttcactt ttggcctgat tgagattgtg ccccaaatgc caagcaggat
    17041 ttgtagcgat gtgattaagt gttacattct cactgaaagc agcagcactc tcatttacag
    17101 gcagcatttg cacgccacca cgaaacatta ttacaagcat tgcagcggaa acaataaatt
    17161 gaagtaatga ttgaataatt gcaagtttgt tatcaaaaaa atgttttgta aattttctga
    17221 atacaaacca tgtaagtata aataaaagta taatacatat tattcccaca atcaattcaa
    17281 aattgttcac tgacaaaaac atctgccgcc agtctgctaa aaaagataat gctctatagt
    17341 tgattagtac accccatgaa cgataaagga aaagattact tacatgcaat aaaattaaaa
    17401 caaacagaat tattccatta taaacaagaa acaacttttt tataaaagta ttttctgtta
    17461 atgcatggat taacattaaa agtaaaggca gtgccgaaat gtatgcagtt gctgaaatgt
    17521 ctagtctgat accataaaac aatgtggaag gtaattcatt tagttgaatg tttgcggcat
    17581 tatactgaag aaacaagaaa aagatgattc tgaaaatgcc gaagaacagc atccagaaaa
    17641 ttgacagcac aagaaaattt ctaatcaaac gcatgttaat cagccttaat taattttcca
    17701 ttacctgttc cttcatgcac tacgctgtcg ggattgccga agtaataaat gtcgcctaca
    17761 taagaaattt tagcagtgag ccattgctta gctctgacat attctttgtt agcacctttg
    17821 ttttctgtaa aagtaacctc tgtattcagg ttgcttgcat cggcaatgcc attgccgtta
    17881 ttataaaaat aatgtacacc cacataacca tgaaagacaa aatcacccgg tcctgtatga
    17941 atgttagttc tcacagctcc ggtgttaaaa agaaaagtca ccttgcccga tgcaccccaa
    18001 ttgttgaacg taaactcatt atgtcgaatg gtgtccatca ggtcaatgtt tcctgaacca
    18061 tttactgtta gttctgcaat atcaggcatc caaactttga cagtaaattt attcttaaaa
    18121 cttctaaccc aattacatgc attttcgttt ctgatgtaca gcatgctgtc ttttacttct
    18181 gttgtaatgc cttcaatcaa ttttgaacct gccattactt caacttgata atcataaccc
    18241 ggataaattt ttacatctac attgttgctt gctttaattc ggttaaatcc ggttatattt
    18301 cttatttcag atttgtcatt tcctgtccct ttaaagcaat caaacatgtg gtctttctga
    18361 caggaacaga agacaagcaa cagaagaaaa caaaaccagt taatgatttt tgccataggc
    18421 cagaataatg tcttttacca aacgatgtcg gacaacatca cctgcatcaa gagtgataaa
    18481 gtcaatgcct ttaatgtttt tcagaatttt cattgcctga atcaaccctg aaggttgttt
    18541 gttaggtaag tcaatttgtg ttacatcgcc tgtgacaata aattttgcag caggccccat
    18601 tcttgtaaga aacattttta gctgactctc tgtagtattt tgtgcttcgt caagaattac
    18661 aaatgcatgg tctaatgttc ggccacgcat aaatgccaat ggtgctacct caataactcc
    18721 gttttctata aaggtcttta acttctcacc cggaatcata tcaaacaatg catcgtaaag
    18781 cggacgcaga taaggatcaa ttttttcttt taagtcacct ggtaaaaaac caagattttc
    18841 tcctgcttca acagcaggac gtgtaagaac tattctcttg atttctttat tctttaatgc
    18901 gcgtactgcc agtgctacgg cagtataggt ttttccggtg cctgcaggac ctattgcaaa
    18961 cacaatgtcg tttttattgc aactgtcaac cattttttgc tgattggcag tacgtgcttt
    19021 tacaacaaca ccatttggtc caaagaccaa aacatcactg ccatttccgg atggtggatt
    19081 tactaaagta tcagagtttt gtccgtcagc cattaacttt tccaatacct gttcatcaaa
    19141 ttttccgtag tgatgaaaat attcaatggc accaatgatt ttttttgcca cccaatgaat
    19201 ttgcttttca tcacccaaaa ttttaagctc attacctctg acaatcagct taaaagcagg
    19261 gaattttttt cttaataaat ctaaattact atcgttgatg ccaaaaaatg ctacaggatt
    19321 ttctaaatca acgggtatgg ttaattcact caattttaca atagtttaat gtatgcaaac
    19381 ctaatttttt ttggacacat cagcgattgt ttttcttaaa attccggtaa tcttcactaa
    19441 catcagaaag tttataattt gtcaatacag taagatgtgc ttacagtagt acggatattt
    19501 ttgtgaaatt aattccggca gtattcatgt cattaattac actcacaaca gattggggac
    19561 taaccgatca ctatgtagca gcactaaagg gcgaattgct gagcatgttg ccgggtgtga
    19621 atattgttga cataagccat cagattccgg cttacgattt tactaaggct gcctttgttt
    19681 ttaaaaatgc attttacttt tttccgcctc aaacaattca tttaatctgt gttgacaaac
    19741 aatctaagaa aacagaagac acacattcag ggtggatggt tattcaatat ggagaaagca
    19801 ttattgtttg ccgcaataac ggttttttta ccttggtgag taagcaagag ccattaaagt
    19861 cggtttacat tgaaggttca gaagaggtga gtgtggcaca tgagaaaagt tttttagtta
    19921 ccctgctaca tgatttggtt caccataaac ctttggaaga aataggacag ccgataaaat
    19981 ctttttatca atcatcaatg tttcagccat cgcacgatgc caacaccatt cagggaacgg
    20041 ttattcatat tgatacttac ggcaatgcta ttaccaatat tgaaaaggaa tattttgaga
    20101 atgtagtagg agcaaaacga tttgaaattg atatgcctcg ccacggtgat gcaattacta
    20161 aaatatataa tcgctatgat gatgttactc ccggacagtt ggtagcgctg tttaatgcat
    20221 cgggctatct tgaaattgcc ttaaatcagg gtgatatttc attgttgtac ggacttgagt
    20281 atggagataa aatcagaatt tttctgcaat gataacacgt attgtacgat tgcccgttaa
    20341 acctgatgag ggcaaaaatt ttgaacaact atttgataca ataaaagaaa aaattgcagc
    20401 ctttgatgga tgtttgtcag ttgaattgca taaagatgta gcttcgtctg attattttac
    20461 ctacagcctt tggcagagtg aaagccattt gcaagcctat ctcgactcat ctttttttac
    20521 agacgtttgg cctaatgcta agaaactgtt gcatcaacgt gccaatgcat ggtcgcttga
    20581 gaaagttttg aatgtttgga aagttgctaa gttttgaaag ttagcaagtt tgaaagttta
    20641 gaatgtttga aagttttgaa tgtttgaaag ttttgaatgt tgagatttaa attacactga
    20701 caagggcggt gcgacatgac aggtgaaatt aactgttgct tgaatgatag atgggcacaa
    20761 gtccgaagac ttgtgccaga ggggtttaga atgttgaaat ttaaattaca ctgacaaggg
    20821 tggtacgatc tgagaggtga aactgaattt gttctatttt cataaaaaaa gtattaaccc
    20881 ttcaatgtgt ttatattatt tcaaagtcct ctgaaatgcg agagactttg aaagtacgga
    20941 aacccacatg cactatacca tgtgttacct gctgggcttt ttccattgtt tgttaaatac
    21001 atattcaacc atttcttcaa tattaactat ttgtcttgtt gatgcattca tccgaatata
    21061 tagttctttc tcaatgaatt catttttttt gtttttaaga aatattggtt tgtaactttc
    21121 ggtaataact attactaaaa tatctttacc ctctactttc ttaattgata aatctactaa
    21181 tggctttatg gataagtcaa aaaaataggc caaaagtgag tcaaattcta ataatagctt
    21241 atccttttca ttttctccat gaaacaatga ataatcataa ttttctaatc cttgaatatt
    21301 accgttatca tttactccaa taaataaaac tccgccattt gaatttagaa atccgcaaat
    21361 agccttggct attaaatatt taacacctat tcctgcggct tgagttttaa aattataaag
    21421 taaacatggt ttaaattcga cttgattact ttcgcctctt ttaataatac ttagtaaatc
    21481 tatgccctca ttaatatcag tttcttctgt atcttgattt gaatcataaa tctcattata
    21541 cctttcaata gtatcccaaa cataaggtgt ttttagaatt gttctttttg ctccgccttc
    21601 ttcatcttct cctgattctg catagtctaa tccataatga taaactgtcg tttttaattc
    21661 accaacattt atttgataat attcataaag aggaacttct tgcatttcaa ggctatcaat
    21721 taaattttga ggttctaaat catacatgga agaagatacc ctgtgtattt caaactcagt
    21781 ttcaacttta ccctcaaatt caaatgcatc aattacgttt ttagctatgt cgaggtcatt
    21841 aacaactaaa taaacagaga tttcttcttt atattggtta tatacctttt taaactcaaa
    21901 ataatcctca gagttttctt ttttatcaag gatttcgttt atgaaagaag gttgagtaat
    21961 tacgaaattt ccttgattac tttttttaag tctcccaatt tgttccaaaa aagataaata
    22021 ttcattaaac tcttcaaaag catttgctct gttttctaat ggtgttttat catcaaattt
    22081 ttttataaat gattcatttc gaacattatt ttttttatca aacgctaaaa gtttgacacg
    22141 aacttcgtaa tgaaaagggt atttttttgt cattttataa atttaagaat tcttttaatt
    22201 cagtgtattt ttcaatcttc gattcgcttg taattgaatt cccatgtgga aaagctatat
    22261 tgtaaaaagt tgtattacaa ttttcaatta atctcccaag ttttcccgta tttgattcag
    22321 gaacagtgta ccctaaatgg ctaataaatt tctttaaaac cttaccatga agcagtactg
    22381 ttgtctttgg tttatagtta ttaataacat ttttcaatct tatcaggtca gcttttttag
    22441 gtttaatttt agcactatta ctttctgcaa tttcagttat taaatcagtg attccaaaat
    22501 tccaattttg atacttatat ttattcgcgc caaaaatcaa ttcatctgca tttgatttgt
    22561 caactagctt agttaacaat atagattcgt aaagttgatt cccaaaagct tggtttacag
    22621 acaagtagtg tccgttttcg cttgaacctt ttgctggatt taatccaaca aataaaatgt
    22681 caagattttt tcttagaaac ggtatcaatg tcgattcttg catagtcgtt ttttttctta
    22741 gccttgcagg taacgtcaat ctgtgaaaaa aatatatcca caaacttatt taaaaataag
    22801 gaagtaatgt aattgtaatc agattaatta tttacaataa atgcctacga ttcccggaat
    22861 tgaccgccac caaatcatct ttaccgactt agaaagacaa atctcaaaag acaatgtaat
    22921 ccgttttata gatgcttttg tagataagat ggatttaaaa caattggaca tcctgtgaga
    22981 gtggacacca aactaccctg tggtagattt tcttcaaaaa acgaacttta tataaaccta
    23041 tttaagtctt tctatttttc agttcaaaaa atggttgcat aaaatcagtt tgtcttaaaa
    23101 ggcaaacaat ggcttttaga gtagagtata ggattgtttt ttacagaaaa agccttcttt
    23161 cacagcctga cggttcgggg cttaacgaag tggtggattt cgaagcacaa aactttcaat
    23221 tcaccacaaa tgttgatgtg aggtagaata ttcggttaac cacgttaccc gccaatttct
    23281 tgtaggtgat gttataagtt gttttctatt tgtcctttgt gtcaattagt tcaaaaatca
    23341 actttgtcaa tgtcttcacg tcactatcaa tagttccttt catatatctc tcgtaaacac
    23401 ttaagttgtc aggtgggttg agattgagtg tcaagtcttt ttctaatgcc aacagtctta
    23461 aaaactctct ttgtgttcgt ccgtttccat ctctgaatgg gtgtaaataa ttaacgttat
    23521 ctaaaatttc tgtcaatttc tctgataaca tttttttgtt gtctttcggg attttcttga
    23581 aatcggcaat taacaggtca atgtatctga aagcattgtc aaagtgtgaa gtcgggaaaa
    23641 attgtttccc atctttactt atttcaacaa ttcgtttctt ccctgcccaa gcataaatat
    23701 cttgaaacaa gtgtctatga atttcaaaaa ggctatcaat acctttgatt tttgtagggt
    23761 tttcttaaag ttcttgaagt cgttttgtta ccgcaccact ctccacaaaa agcaaaacat
    23821 cagcgtctgt aatatcttgt aaattcctta aaagtcctgt cttagggtcg gtataggtaa
    23881 agtcagggtc tatgtatttg taggaattag acataggctt tttgtttggc gaatgccaca
    23941 agttctgtta gagatatttt tccagtaaca tagtcgcgta taatttctat tccttttggt
    24001 gtaggtttaa atccttcaaa catactgttt cccaaagcat ttgcagtgct ttcaagcgtt
    24061 ttcaagtctg aaaattttac ccccattatg gtcaggttat ttctgtctat ttctattgtg
    24121 ttaaacatat ctttcagtat taaaatactt ttcaaattta caaaattttt aggtcaaatt
    24181 gtttgttttc tggctcttta aaatggctta caacgtcagt tttccgatag ctatcggaat
    24241 taaaactaat cattagctca aaagtaaaaa gaaaaatgat tcaagaaagc attagcagtt
    24301 aattaggctg tattaaaatt aattgtttgt gttaaatcac attgtcaggg gcggtgcgac
    24361 ctgaggtgaa attaaaaact cttgcgaagc aggaaggcat aattcaaaaa tgctttcctc
    24421 ttcaaaagaa aatttatcgc tgtacctgag tagggggatt tacataggaa ctgctgttaa
    24481 cggttcgggc tttctgtttt taatttgtat agttgtgttt tcaatgtgtt gaatagtttg
    24541 atttctaatg tcatcgtgtc taaattttct gtagtccaaa atggaatttc cattgcggtt
    24601 attgtgattt tgtcatctat gctgaaacct gaattaattt caaagatgtc tggctcgttt
    24661 atatcgtctg ttatgtttgg atataaaagg aaaatgtctg tgcaacctct acgaaaagca
    24721 tagcttgtca tttgataaag gtcggcttgt gctattcctt tttttatgtc gtttttgaag
    24781 tcgaacgaac gaagtttgta tttcgtgtca ataattactt ttcgtctgtc tgctgatttg
    24841 gaggtcaaaa atatatcatg ttgcatatta aatgctttcg gattacttga caaatattcg
    24901 tttgactttt gatattctac tttccaatca ttagaaaatt tgtcggctaa aaaacccgca
    24961 gcaaaatctt caaaaatgta ttccattgga aacaacaagc accattgaga caagtcataa
    25021 gtattacttg aataaagttg ttgattcaaa attaatttac aactgtcaag aactttgttg
    25081 taatcttcaa aaaaagaatt gagggtaata ttttcaactt cgtaactatt gcaaggaatg
    25141 tcttcaactt cgtctaaaat gaaaacaact tcttgaagca tttgaatgtt ttccgcaaac
    25201 cttgtttgat tcattaaaag tcgtgaacaa tacttgatta cacgatttac tttgttgtca
    25261 aacaaaaatg gttcgtagtc gcattctatg ttttgaaaat ttccacttac caaactgttt
    25321 gcaatgtatc ttttaaaatt tattgaacct cttggcgttt gcattgcttc ttcaacaggt
    25381 tggtacattg ttaaaggttg atttgaaact gtttccaaaa attgatttgc aattaagttt
    25441 ataatcaatt caggaaattc gtcaattgaa aatctgtcaa gttgtgcttg actgaaagga
    25501 aatttccatt tgctacaata gttaaaccaa aaaaagatat gacgaagcat atcgtttttg
    25561 tctttgtctg gacaattgcg aaatactttt gggtaaatct caactaaatc gtttgctgtt
    25621 tgaataaagc caacatagtt gtttgctcta atttcattat ttgaaaagcg taaaaatggc
    25681 tgatagtttt tatcggtttc gttttcttct aattcatctg ttaaatccgt gaaattacgt
    25741 tgtcgccaaa ttgattttag cgaatgaaat agggctgtag aattttcaac ttttacccat
    25801 ttatcatatt caaacaacgt ccgtttcatt tatcgtagcg atattgtcca attaaattgt
    25861 gtagtgtcgt attcaacttt ccaagttgtt tcattgaata tgtctgaaac gattttcgtt
    25921 tttcctgaaa aatattccat taaaagtgga ataactttgt ttcttaaagt tgtttcaatc
    25981 gcttcgtctt tcataaaata ggcgtgacca atcaaataat cggcagaatt tttcagtttg
    26041 taaatttctt cgtttatttt ttgaagtaac gttttcgctt cagattttaa tttttcgttt
    26101 tcgtaatctg gataataacc aataaactca aatcttctac gcaaagcaat atcaatgaga
    26161 gcaatagact tgtcagccgt attcattgtg ccgataagat aaagatttgg cggaacgcca
    26221 aattcttttt ctccatttgg caatgaaatt ttcaattcat tgggtttccc gagccttttg
    26281 tcatcttcaa gcaatgtgat tagttcgcca aaaacttttg aaatgtttgc acgatttatt
    26341 tcgtcaatga ttaaaacgaa gttttttaat ggttcttttt gaacagttgt tgattggaat
    26401 ttatatccct tttctttaag aaattttctg aatttctcga ttagttcaaa actgtatgtg
    26461 gcgtgttgtt tctctaaact tggtacgttc tctaattttt ttatgtcttg tcggttttgt
    26521 acgtcagcaa gataaatgtt gattaatgca tcatagtgca ttccaattcg ctgatttttc
    26581 caaagctcac cattttcttt ttcgcctgtg taaatgaaaa cttcgtcctc aactgaataa
    26641 attgaaacat tgttttttat ggcaaatttt ccgttttctt caatttcggc ttcaacatac
    26701 tctttgaact cttcaaattt ttcatttacc caattctctt ttgaaatcgt ttgttcgtct
    26761 ttcaaagagt tctcgtagtt ttctcttgct cgtttagcta tttcgtaaaa tataccataa
    26821 tgctttttga aacttaattc acttgtcgtt tcactaacat ttggtcgaat accaaccata
    26881 aaatcttcat aggaataatt ttgatgaaat gttacaaatt caatttgtcc ttgtttacgc
    26941 aattcatcaa attgtgtctt gttagaaacg tggtcagttg acgaacctgt tgcgatagca
    27001 acggctttgt caatactgtt atatgtcttg cctgtgccgg gcggtccata caaaatggaa
    27061 tttaacggca tatttgtaat taaaagagtt tttgcaattt gagtttttgg taattgagct
    27121 ataaagtctg agtaactttt gattgcagca gaataaaatc tagaagcacc ataagaaggt
    27181 gcttgttcgt aataatattt accatcttct ttttcttgtt cgtttagtaa gtcttgataa
    27241 agagcattta aaacagtgag tttgtcttct tcaaaaattt ctttgtgtgt aatttcagaa
    27301 agaatttcaa tagctctgat gtaagaacct gcttttttac ttccttcctt atttgtgcta
    27361 ttcagccaag ttgtgtattg ctctttgtaa ttcattcttt tattgtgtct gttattccac
    27421 agttgaagtg ccttttgata aacttcacga ctttgaacac gaactatacc ttgttgaagt
    27481 tgatattgga aaatttcatc ttccgctaat tcataccatt cggatttagc aaagtttatc
    27541 caatcttcat attctatttg gaaatcatta aacggttttt caagttcacg atttgttgtt
    27601 accgaaattg gtgtttcttc aatttttgcg attgctttta cagtgtgtcc ttcacaaact
    27661 ataattaaat cgccaatttt gtattttttg tcatcaacac caattgtaat tcctttgtct
    27721 ttgataagat tataaaaact tggctttccc tttccccaat tacaaccaag cttccaaaga
    27781 attcctgtca attcggattc ttgttttgcc tttatttcgt aacccaatga attaaagtaa
    27841 ttgtttgttg gttcgccacc actgcgctcc caaatatgtt ctccgtttaa caattcgtgt
    27901 gcatatctca taatctcttt gggaggatat gctttgccgt taattactac atcaaacttt
    27961 gtagatggct caagttgaat attttctctt tcgattcttt caactgcttt ttgaattaag
    28021 tctcttgtta ttttgtctgg ttcaaatgcc attttacttt gtcgttgtta tttgaatgtc
    28081 tattgttgtt ttgtcgtcat agcctgaccg ctaacggttc agcgtgtttg cgaagtgcct
    28141 ttcaaacttg tttgaaaaag g
//
LOCUS       DAOOOI010000020        27525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_307_length_27525_cov_20.849618, whole genome shotgun sequence.
ACCESSION   DAOOOI010000020 DAOOOI010000000
VERSION     DAOOOI010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 27525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27525
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_307_length_27525_cov_20.849618"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..341
                     /locus_tag="PKX59_04420"
     CDS             69..341
                     /locus_tag="PKX59_04420"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOZ90144.1"
                     /translation="MSLTTHFVFKIIRNVKNVPKKFLEHLTGTEGLYEIRIEFESNIY
                     RIFCCFDKGNIVVLFNGFQKKSQRTPAKEIALALKLKDEYFNLKIR"
     gene            338..646
                     /locus_tag="PKX59_04425"
     CDS             338..646
                     /locus_tag="PKX59_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019967666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOZ90145.1"
                     /translation="MRKKKKIREAKSFDELLDIKYGKIGSDNRDDFEEKAQYFVISEV
                     LKEARKEANMTQEQLAEKVGTKKSYISRLENGKCDIQLSTLYRIFEFGLGKRVNLIFG
                     "
     gene            714..1121
                     /locus_tag="PKX59_04430"
     CDS             714..1121
                     /locus_tag="PKX59_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90146.1"
                     /translation="MSEIKIKEAPVVKPQIKIDTETKQRLQSEVSEESQVIVHCSCEG
                     GWDKKIRIWRSTFLYAKGSTHRSKLLHAENITFYPTWMSVECGETKYFTLIFSGLPKG
                     CKVFDLVEEIPERGGFEVRNMVRNDSDVYAVEV"
     gene            1297..2181
                     /locus_tag="PKX59_04435"
     CDS             1297..2181
                     /locus_tag="PKX59_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4007 family protein"
                     /protein_id="HOZ90147.1"
                     /translation="MSTASVDTIKYTFSGHDSFQCRQLWLKKGYDYILSNKSFNDEDA
                     VVKLGVGKNMVSAIRYWMKAFNIIDGKDKPTEFGTKLFDDKEGYDPFLEDDASLWLLH
                     YQLIKTGLASTYNIIFNEFRKEKLFFNKDTFVNYLKRIREAEKNLQFNDNTVADDFIV
                     FVKMYQRSETSKDVEDSFSGILSEIELLKTIGKGKDEQYQIENNERDKLPEAVLLFSI
                     LDNPTYGNSISLNTLEFDNNSPGLIFCLNRSGLTNKISEIVHDNKQITFTDHAGIKEL
                     QFKNKPNAYSILDTYYGK"
     gene            2171..5389
                     /locus_tag="PKX59_04440"
     CDS             2171..5389
                     /locus_tag="PKX59_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90148.1"
                     /translation="MANNFTTSANILRDTERDFNYIPTPNAKQVVSQVVNDFKKGIRS
                     FNIIGTYGTGKSSFLVALEQSIRGTKRYFDPNFIAHPKVDFVKIVGSYTSIVEQFADI
                     FEVSINKNKHENILSEIFNRYHSLGKNNGVLFLFLDEFGKFLEYASKHNPENELYFIQ
                     QLAEFCNNPKYNIVLITTVHQSFESYAYSLTSTQKQEWTKVKGRFREITFNEPVEQLL
                     YLAAEHVAENFESKPSKTAVEKCLKLATTTKAFNFNKDFLNEIATKLFPLDVLSANIL
                     TLSLQRYGQNERSLFSFLESTDHTGLSKFNKRENPFYNLSCVYDYLNFNFYSFLTSKY
                     NPDFSAWSSIRSSLEEVERAFDSSINDYAKAIKAIGLLNIFSASGAILDENFLSNYLE
                     TACGVGNASSIIDNLEKKRIIRFRSHSKRYILFEGTDLDIQTALIEAGNKISEVSDVT
                     TLLNKYFQFTPVLAKLYSYEKGTPRFFEFVISEHPITRVPDGEIDGFVNLVFNSKMKV
                     AEVEEASRTQKEAIVYCFFKNSTEIKSLLFEIEKIQKVIEENKEDKVAKRELENIVES
                     QIRLLNHYITDSIYTGSKEVKWFFKGDEKKIADKKDFNKLLSQVCSSVYDATPVFKNE
                     LVNKHRISASIHTAKRNYFKALANNWDKENLGFEEAKFPPEKTIYLSLLKENGISPIR
                     ENSFKVPTIDKHSSFNKLWKASEKFIENAKLEQKNISDFVSLLSKRPLKLKQGLIDFW
                     IPTFLFIRRDDFAIFNADGYIPTLSDENLELIAKNPENYSIKTFDIEGVKLDIFNSYR
                     TFLNQSTELKFDNNSFIETIKPFIVFYKQLPEYSKQTKRLSNAALKIRTAIATSKDPE
                     ETFFEAFPNALEISLGTLQKDKSKLQSYTTSLQNAVKELRTSYDGLIKRFEEFICNEF
                     VGKKVDFEEYKEHLQQRFAKLKKHLLLANQKTFVQRIDSALDDKKAWLNSIAQAITGR
                     TLEMSTDEDEILLYEKFKSMILELDSLTNISKADIDENNEEVIGVKIDTFFSTINPKV
                     VRVPKKKAKEIEMIKIALKKKLSSDKTSNIAAVLNLLKELLQ"
     gene            5386..6489
                     /locus_tag="PKX59_04445"
     CDS             5386..6489
                     /locus_tag="PKX59_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018919963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HOZ90149.1"
                     /translation="MNKVRHVLGISGGKDSAALAIYMKTKYPSLDIEFYSCDTGKELD
                     ETYQLIKNLEVYLGIKINLLQAAQNSSEDPFDHFLKMYGGFLPSANSRWCTKKLKLEP
                     FEKYVGADLVISYVGIRGDEEREGYISTKKNIQSIFPFRKNIWSEDVVAKLFSNQNFE
                     KLQTLASNVNFGRFQDKATQIIARKLEPSFTQAQKLNLLLDTDVKGFNHLVFEFLKTT
                     DYPLSNESSFVLLDNEDVLLRADIFKILEDSGVGVPAYYNKLDFDVNGKTGQYSRSRS
                     GCYFCFFQQKIEWVWLYEQHPDLYKKAMQYEKDGYTWGQTESLEELIQPARMAQIKTE
                     YMKRMEKKSQSKSPYLIDILSEEEGVGCAACFI"
     gene            6517..6732
                     /locus_tag="PKX59_04450"
     CDS             6517..6732
                     /locus_tag="PKX59_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90150.1"
                     /translation="MANKFQNWIEQYPDYLVNQKTKWVVRDNSVVDDKQWVILDDRGI
                     MISCEWKDNKEDCMEEADRICVEELFV"
     gene            6732..7883
                     /locus_tag="PKX59_04455"
     CDS             6732..7883
                     /locus_tag="PKX59_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOZ90151.1"
                     /translation="MNSLENIIYFDNNATTPIDKRVLDAMLPFLTTNFANPSSTHHFG
                     ITANEAVKKARTQVADLIGSDKNEIIFTSGSTEAINIALKGIAENYSAKGKHIITVST
                     EHYAVLDTCKHLGSRGYEVTYLPVSKDGLIDLQELKNSLRGDTILVCVMYVNNETGVI
                     QPIKEIASLAHEAGALFMTDATQAAGKIEIDVDTLGIDLMSMSGHKMYAPKGVGALYV
                     RQCMNRVKIPAYIHGGGHEQGVRSGTLNVAGIVALAEACEIAQQEMKQDAERISALRN
                     ELENELLKIEGTFVNGKTESKIFNTTNICFRGNDANVLIGRMKNVAVSNGSACTSAVV
                     EPSHVLTAMGLNGDDAFASIRFSLGRMNTLQEVKEVQTIISRTIIPTLI"
     gene            7895..9610
                     /locus_tag="PKX59_04460"
     CDS             7895..9610
                     /locus_tag="PKX59_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ90152.1"
                     /translation="MAEEQIRFVSDATALLSMRDSDFDAYSAYGEVIDNSIQADATNI
                     KIMVKSYHEARQRVGDYQVVDWIAFGDDGNGMDMNTLHRCLQLGYSSRYNDRSGIGRF
                     GVGMTLGAINQCKKVEVYSKTLKSKWLYTYIDIEDITSSPAKMNAIPTPTEKEIPFHL
                     AELVTDKQGTLVIWTKYDKQPVSADALEDEMAVWIGRTFRKFIWGEVEVNGKKNKVNI
                     EINNKKVLAVDPLHLKNDRFPTDVPSYKTELIEFPWHVPFEDRKTDEEETSEIKIQMT
                     LIDKSLRDYQGVGNAKETKDRYIDRNEGISILRNGREVFYGEIPHWKPAFEEIDRWWG
                     CEISFDAILDKSFAVKNIKRGAVPVATLKQTINDKIVPTIKVFREEVSEHWKRRRLER
                     TQEKLAQQTEDATATGHEEAESVAKKSETQKTKATAETQANALELASQIKANETAERQ
                     AAWATKFASQPYTIIDESWKSPEFIEIHPLGGTDVIKYNLKHPFIEELYSLVEQIQNS
                     EIDQELAMKLKSLIDLLIISYAKSESMFDKQKEFKAEKFFEDLKINWGQYLKSYLETW
                     KSENI"
     gene            9612..11252
                     /locus_tag="PKX59_04465"
     CDS             9612..11252
                     /locus_tag="PKX59_04465"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOZ90153.1"
                     /translation="MIFNNIPKTQLQELIGQSVLDRLEKIIPVLKGDDTDPNEIYRKE
                     TLVKIFSSFSNSNLFRDKDFFKGLLETTPVEIIDEICSATGISIEGKVFEQKVDSILK
                     KGWKDFEFCSKFLKVAELPDTFLPVQQEPFIAEEDCFPPVTPFKSLKDYQIGVYQQSI
                     ELVKIPNSRFIVQMPTGSGKTRTSMEIITKVLCKNPEGSVVFWLAHSEELCEQAVQCF
                     KEVWEHVGNKKVKLFRVWAKGNLSFTFNESAFMVGGFQKLHSLLRKNSTPFDEIKKRI
                     VLLVIDEAHKVLAPTYTEATRALMGEKTKVIGLTATPGRHIDDLEQNKLLADFFLNKI
                     ITIKTADNNSVIQFLRDKKVLSKAAFNPLITDIRYVLDEKQKKHLQTFYDFPEGLLKK
                     IGNDDVRNLEIVKRIDREFTHSKPQMIFFACSIEQSKFICSLLVYLGYKAAHVDGGTE
                     KPMRQKLISDFKNGEIQVICNYGVLSTGFDAPKTDVVFISRPTRSIVLYSQMIGRGLR
                     GLAIGGTEKCKVITVRDNIMGLPDEDDIFDYFDEYFEN"
     gene            11255..12205
                     /locus_tag="PKX59_04470"
     CDS             11255..12205
                     /locus_tag="PKX59_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOZ90154.1"
                     /translation="MLYKNGVDLQPKLSEYISNSQALFIFSPYIKLETLKALIDGNEN
                     VKVVFVRWETKDLILGASDLEIYPYLKAKGITLYRNSRLHLKAYLDEYKNCFLTSANI
                     SSRALNLPLYSNYNYEIGTVVENLEIADRLYFQIIESDSILITDNIYKQLSEQLPEKK
                     KEFPDEVEFHFKFEAPDKDFLISALPMTYSIETLYRIYEDKEFVNDLELNCVLHDLAI
                     YKIPLSLPSTEFREKLTEAFFSHRFVKGFLQNLESTGEIYFGTAKDWIHKNCTDVPTP
                     RKWEITENIQILYKWIVKLGNGRYKVDVPGSHSERLSIVI"
     gene            complement(12267..12521)
                     /locus_tag="PKX59_04475"
     CDS             complement(12267..12521)
                     /locus_tag="PKX59_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90155.1"
                     /translation="MWKCKTTDNHNYFIVERSANGTDFSEAGLVKANRNNQINYYSFV
                     DRTDAGTTNYYRIKLKSFNGDETQYGEIVKAGKEDVANSR"
     gene            complement(12532..13323)
                     /locus_tag="PKX59_04480"
     CDS             complement(12532..13323)
                     /locus_tag="PKX59_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HOZ90156.1"
                     /translation="MEISLYHLNQDDFISLTDLARHKDSERTDYIIQNWLRNRNTIEL
                     LGFWEQLNNPNFNPIEFEGFRKQAGLNSFVLTSKQWIEKTGAIGIISKSGRYGGTFAH
                     KDIAFEFASWISIEFKLYLIKEFQRLKEDENNRLKLEWNLQRTLAKVNYHIHTDAIKE
                     KLIPGSISSKQASFTYATEADLLNVALFGITSKEWRDSNPTLEGNIRDHATIEQLVVL
                     SNLESINAILIHQGLAQKDRLLQLNQIAITQMKSLLNAKGLKKLK"
     gene            complement(13539..14273)
                     /locus_tag="PKX59_04485"
     CDS             complement(13539..14273)
                     /locus_tag="PKX59_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90157.1"
                     /translation="MFVKAGDKTIYSVDFKKAETFKVSGGVINSEYWEVRNDSAIFTS
                     NNRLCNEKGNYRYQVEVTLTADGKLNADDQCIIYIMQNEAIAKSFVVKGSDMTESKKF
                     KYEVEGEKSPVLKVNIVLKSLGVDRAWRLTDNAVSIIAISKDDDPELKVIQHANTSGV
                     VWKCKTTDNHNYFIVERSANGTDFSEAGLVKANRNNQINYYSFVDRTDAGTTNYYRIK
                     LKSFNGDEMQYGEIVKAGKEDVANSR"
     gene            14394..15521
                     /gene="hemW"
                     /locus_tag="PKX59_04490"
     CDS             14394..15521
                     /gene="hemW"
                     /locus_tag="PKX59_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOZ90158.1"
                     /translation="MSGVYIHIPFCSKACTYCDFHFSTQLRNKNAFIEALLHEIEVQA
                     RYIDEAVNTIYFGGGTPSLLSAAEINSIIGKLDKVFKLDVKEITLEANPDNLSEKYLK
                     ELKSTAVNRLSIGVQSFEEKRLLWMNRSHTTKQAIFAIESALQMGFNVSADLIFALPE
                     MSIDEWQRQLQKMISLKPHHISAYSLTLEERTVYHHQVAKKKVKELDDVNSEEQFLQA
                     HQLLSNAGYHHYEISNYASAGHVALHNSSYWNGKPYLGLGPSAHSFNGKMRQWNVSNN
                     TAYIKALSAGKLSFESEVLTEVDRYNEYVMTGLRTDKGIEEKQWNELMPSLYKESLQK
                     EMNELVNQQFLVRDASRIFIPLQHWYKADQLISRLFATENS"
     gene            15527..16279
                     /locus_tag="PKX59_04495"
     CDS             15527..16279
                     /locus_tag="PKX59_04495"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOZ90159.1"
                     /translation="MIAHLKIGDEEYSAKLNEPIDISIPLHSGNDNVNAWYAPPPEIV
                     PVRMDSWVGEVAQGASVNFRNVFFNPHAHGTHTECVGHITKEWVSLNKVLKQFFFSAK
                     LITVLPMIVDGDMIITKEDVVQAWKNCGAEALVIRTLPNDNSKQSRHYSNTNPPYLHH
                     EAAAWMAEKNIKHLLLDLPSVDREQDGGKLLAHNAFWSTGKNIRYDCTISEMIFVPDH
                     IADGLYLLNIQITALENDASPSKPLLFQLTKK"
     gene            16291..16875
                     /locus_tag="PKX59_04500"
     CDS             16291..16875
                     /locus_tag="PKX59_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOZ90160.1"
                     /translation="MKKLLNYFFQGLLVVVPIAVTILVIYKIITWIDNLLPFQIPVKL
                     PGIGEVSIPGLGILTIVVAVTAFGYLAKFFVANPFFVLLERMLERTPLLKIIYTSVKD
                     LIEAFVGEKKRFNQPVLVTVNKVSGVQRIGFITQKDLSVLGISSNKMAVYLPFSYGFN
                     GQLVIVPAENVEKIDASGTEMMKFVISGGVTEVD"
     gene            16886..18484
                     /locus_tag="PKX59_04505"
     CDS             16886..18484
                     /locus_tag="PKX59_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90161.1"
                     /translation="MNIFRFALFAAMLILMGSGVCAQDYFIPLNREWNLRYEPFLQTT
                     KSNNVHTSIKPYRHDEIIAVANIDSVDTANFPISRFSKTLVGRKIFKEHLVQIKDDDF
                     LIHADFIFEGRIGKDLPETETLFTNSRGFWLGGTAGKRFSFSTTFFENQARFPSYIDT
                     FVTRYNIIPGEGRIKKQYGSYDYSNVTGTISYTINRHFNVQFGHDKNFIGDGYRSLLL
                     SDNANSYPFLKISTTAWKVKYVNLFTVLTDLQFPAPNDIAFKKKYASFHYLDLNIGKH
                     ASFGILESVVWSGDSARGQGFDINYMNPFVFIRPVEFSIGSPDNMMLGFNLKVKLFSK
                     LVFYGQLMLDEFKLSEIRDGNGWYANKQAFQLGLKSYDLPIKNLDVLTEFNYVRPFTY
                     SHRTTFTNYAHFNQSLAHPLGSNFYESMSLINYKYKNFIFRFQFNYAETASDTGNANL
                     GNDIFKSYETYTFAYGNRMLQGQRHNLIYSDIRAGYLINPRTNLCFEIGYSYRRMTTN
                     SEAATTNWVYAGIRTALFNRYHDF"
     gene            18593..19663
                     /locus_tag="PKX59_04510"
     CDS             18593..19663
                     /locus_tag="PKX59_04510"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOZ90162.1"
                     /translation="MINIAFSCITAFIITFLAIPSIIKVAVIKNLFDEPGGRKAHKAS
                     VPTLGGLAIFAGVVFAFTFWSAGFNLQDTQYIIAALIVMFFIGIKDDIVELPAMKKLY
                     GQIVAAVIIVLFANIRITGLYGIFGIDQVPYWFSLLFSMFTILLIINAFNLIDGVDGL
                     AAGIGAIAAFTYGIYFYNYNEIALCILSFSLFSSLLAFLVYNFAPANIFMGDTGSLII
                     GLILSILTIKFIEMSFAPRPENFPFQSSPAMAVAILVLPLFDLLRVFIVRILNKRSPF
                     QADRLHIHHLLLDMGLNHREVSITLYLVNIGFILVALALRSFGALALLFVISSLALIL
                     SLVPYYLKYQAGRSGAVSRSTK"
     gene            19811..21370
                     /locus_tag="PKX59_04515"
     CDS             19811..21370
                     /locus_tag="PKX59_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOZ90163.1"
                     /translation="MRLKKLVYSIALLLPFIGNAQVVENAQPQSGDPIAAMLDSLVSI
                     RNVVRLSETNLQQPPVFSENDAVLSDAFIQERISKISTPIPLTYNYQVKNYINLYASQ
                     RAQLTSRVMGLSRLYFPYFEQMLDKYNLPVELKYLAIVESALNPTAVSSAGATGIWQF
                     MFNTGKMYGLNINSYIDERRDVRLATDAACRYFQDMYAIYGDWLLVIASYNCGPGNVN
                     RAIARAGGSKNFWEISRFLPAETRGYVPAFIGATYVMYYAKDYNITPTKPSFTYFDVD
                     TVGVKNKISFRQLSQTLNIPYDVIAFLNPVYKRNMIPGEGNRLYLPTEKISMYLANEE
                     GLYAASGTELQPSSFAIADDVGYVQVKQHVKVRKGENLFTFAKRNDCSAKQVKKWNGL
                     RSNNIKAGQKLVVYATIKQKPAPVENTTATAANDKTDSANVNTTVKADSSSDSETGAV
                     TTESKTTQVSQAQSAASKYIIHLVQPGDTLWNIAKRYEGVTVELLKEANNFTPKSTLK
                     VGSKIKVPTES"
     gene            complement(21389..21910)
                     /locus_tag="PKX59_04520"
     CDS             complement(21389..21910)
                     /locus_tag="PKX59_04520"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOZ90164.1"
                     /translation="MNILDLFLAVPLLWGAYKGFTRGIIYEIAMILGLIAGIYVAFKF
                     SGVVQGWLFQLVNKDSALPAWVGFFIISGIIMAVFILYARLLEGILKAGDLNLFNKII
                     GGVFGLLKYALVLSVFLWLLKSLEPHLNFINSQTKKESKLYDPVLKTSTFLTPALQDI
                     KNEFRQHIEDNNH"
     gene            complement(22007..22930)
                     /locus_tag="PKX59_04525"
     CDS             complement(22007..22930)
                     /locus_tag="PKX59_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOZ90165.1"
                     /translation="MSLLVVGTVAFDAIETPFGKTDKIIGGAATYASLAASYFAPKIN
                     LVGVVGDDFPQSTIQDFNNHNIRTEGLQIIEGEKSFFWSGRYHTDMNSRDTLDTQLNV
                     LANFDPIIPESYQDCEFLMLGNLMPKIQLQVIERLRKRPKLIVMDTMNFWMDMAMDDL
                     LKNISMIDVLTINDAEARQLTNEYSLVKAAKKILNMGPKYLIIKKGEHGALLFHNNEV
                     FFAPALPLEDVFDPTGAGDTFAGGFIGYLAKTRDISFSSMKRAIIYGSAMASFCVEKF
                     GTERIMNLDSDELNERIQEFIDLVQFDITLV"
     gene            23101..23646
                     /locus_tag="PKX59_04530"
     CDS             23101..23646
                     /locus_tag="PKX59_04530"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HOZ90166.1"
                     /translation="MLEQLLNLVKENAGEAIINNQAIPNEQNDAAISQATEGIIGGLK
                     SQLASGNISEVAQFLGGQNNAATTGVTNAISSQVSQGLMEKFGLSGNSAGSIVSSLIP
                     VVLSKLVSKTNDPNDKSFDLNSIFNHLSGGKTQGMDLSNILGSVTGGNASGGGIGNVL
                     GSVMGGDKNSALGGLMDIFKK"
     gene            complement(23787..24221)
                     /locus_tag="PKX59_04535"
     CDS             complement(23787..24221)
                     /locus_tag="PKX59_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HOZ90167.1"
                     /translation="MDSIFGSYTLEEVKLMGKNTMLNHIGIEITSIKNNTIKGKMPVD
                     NRTRQPKGIMHGGASCVLAESLASLGAFLTLKDKSATCVGMEINANHVRPVKEGFVYG
                     TAKPFHLGKKTQVWTIEILNEKQQPVCISRMTIAIGRLHLKI"
     gene            complement(24231..24887)
                     /locus_tag="PKX59_04540"
     CDS             complement(24231..24887)
                     /locus_tag="PKX59_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90168.1"
                     /translation="MSSKKIKYTLLTIFVLLSCSGNVHAQLRDTLAYLFKQRPKPFFQ
                     LDGYNSFVRSRGANAIGFKTGLDFGKRVKFSMGYMKLFTDVVDSVQVKPNKYYRGEVR
                     SNYFTTGIEYVIYRNGPWQVNVPMHFGFGTSYYEYPDGKKANNKALQGNIILFEPAIT
                     GHYKLIRWVGLGFGVGYRVMLKNNHQLKDNLSSPIYILRLKIFVDEIYRTVFPPKQKK
                     "
     gene            25089..26321
                     /gene="odhB"
                     /locus_tag="PKX59_04545"
     CDS             25089..26321
                     /gene="odhB"
                     /locus_tag="PKX59_04545"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HOZ90169.1"
                     /translation="MKVEIKVPSPGESITEVQIARWIKKDGDYVERDEEIAEIDSDKA
                     TLSLVAEADGVLKTLVGEGENIAVGSVVCTIDTDAKPVAKAEPKKEEKPKEEKPKEVK
                     PTVTVASAKAEGYAAGTPSVAAAKMMKENNVKSESVQATGKGGRITKGDVLQSLQTPA
                     ATQAAPQAREQRREKMTMLRKKLSQRLVAVKQETAMLTTFNEVDMSAIMDIRKKYKDV
                     FKEKHGVGLGFMSFFTKAVCEALKSFPAVNAQIDGEEILYHNYCDVGIAVSAPKGLMV
                     PIIRSAETLSLAQIEQQVIDLATKARDSKLTIDEMTGGTFTITNGGVFGSMLSTPIIN
                     PPQSAILGMHNIVERPVAVNGEVVIRPVMYVALSYDHRIIDGRESVGFLVKVKQMLEE
                     PAKMLFEGNDPIKVLLHL"
     gene            complement(26398..>27525)
                     /locus_tag="PKX59_04550"
     CDS             complement(26398..>27525)
                     /locus_tag="PKX59_04550"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOZ90170.1"
                     /translation="MKVVEQKRNLENQFTTWVNADKKRRLHYGFVMGNLQQSYDNARK
                     YAKLRVYTSEALMGTEILRYAFSFSALDKALNEKEPNADDIKAKAAMLKSRVEDYFEE
                     YDAGVDQKVFAVLVKSFADDVAKDQQSPIFAEMAKKYKGDYNKMAAAVFAKSIFSDKN
                     KVLAFLENPLAKKLKNDPAFKLINTQLSFYDDNFRAPIRTIDLALAANNRLYIEGLRE
                     MMPDKKLYPDANSTMRLSYGSVRAYDPADAVKYSYFTTIEGVMQKMDNSNTEFKVPEK
                     LVELYNKKDYGRYGSNGTLNTCFLTNNDITGGNSGSPVINGNGEIIGLAFDGNWEAMS
                     GDIKYDEEYKRTICVDIRYVLFCIDKFGGAKHIVDEMKVVE"
BASE COUNT         8956 a         4737 c         5562 g         8270 t
ORIGIN      
        1 ccactcacat gaaatcatta tccctctatc gtcaagaata acccactgct tatcgtcaaa
       61 caacactgtt gtctcttaca acccattttg tttttaagat aataagaaat gtaaagaatg
      121 ttccgaaaaa atttctcgag catttaacag gcacagaagg gctttatgaa atcagaatag
      181 agtttgaaag taacatatac agaatatttt gttgttttga caaaggaaac atagtggtat
      241 tgtttaatgg ttttcagaaa aagtcacaga gaacaccagc aaaagaaatt gcattagcat
      301 taaaactaaa agatgaatat tttaatttaa aaataagatg aggaagaaga aaaaaatcag
      361 agaagcaaaa agctttgatg aattgttaga cattaagtac ggaaaaatag gctcagacaa
      421 tcgtgatgat tttgaagaaa aggcgcagta ttttgttatt agcgaagtat tgaaagaagc
      481 acgcaaagaa gcaaatatga cacaagaaca gcttgccgaa aaggttggca ccaagaagag
      541 ttatatttca agattagaga atgggaaatg tgatattcag ctttctacac tctatcggat
      601 ttttgaattt ggacttggca aacgtgttaa tttaattttt ggatagcaca acggttgata
      661 gccttgttac tcaggctgat tcaggcgatt aaaaaattca gtaactttga aacatgtcag
      721 aaattaaaat taaagaagcg cctgttgtta agccgcagat aaaaattgac acagaaacaa
      781 agcaacgatt gcaaagcgaa gtgtcggaag aatcgcaggt gatagttcat tgttcttgtg
      841 aaggtgggtg ggataaaaaa atcagaatct ggaggtcaac atttttatat gcaaaaggct
      901 caacgcacag aagtaagtta ttgcatgccg aaaacattac cttctatcca acatggatga
      961 gtgttgaatg tggagagaca aaatatttca ccctaatttt tagcggactt cctaaaggtt
     1021 gtaaagtttt cgaccttgtt gaagaaattc ccgaacgtgg aggctttgaa gtgagaaata
     1081 tggtaagaaa cgattctgat gtttatgcag tggaggtgtg agggggtatt tcgtaaatag
     1141 taatttttat ggtgaagaag aataaaaaag taaatcaatg acaattaatt gactatcata
     1201 tttgttaata acatatgtat atataaatag gacataccca aaatttattt tattttacga
     1261 gaaattaagt tgacagcatt acaatcaaat aattagatga gcacagcaag tgtggacacc
     1321 ataaaatata ctttctcagg acacgattcc tttcagtgcc gacaactatg gctcaaaaaa
     1381 ggttacgact acattctctc aaataaatct ttcaatgacg aagatgccgt tgtgaagttg
     1441 ggagttggga aaaatatggt ttcagcaatc cgctactgga tgaaagcgtt caacatcatt
     1501 gacggcaaag ataaaccgac agaatttgga acaaaacttt ttgacgacaa ggagggttac
     1561 gacccgtttc ttgaagacga tgcgagtttg tggttgctgc attatcagtt aatcaaaaca
     1621 ggacttgctt caacttacaa catcatcttc aacgagttca gaaaggaaaa acttttcttc
     1681 aacaaagaca catttgtgaa ctacttgaaa cgaattcgtg aagcagaaaa aaatcttcaa
     1741 tttaacgaca acacggtcgc agacgatttc attgtgtttg tgaaaatgta tcagcgtagt
     1801 gaaaccagta aagatgttga ggacagtttc tcaggaatcc tttctgagat tgaattgctg
     1861 aaaacaattg gcaagggcaa agacgaacaa tatcaaattg aaaacaacga aagagacaaa
     1921 ctgccagagg cagttttact cttttcaatt ttggataatc cgacttatgg aaattctatt
     1981 tcactaaaca cattggagtt tgacaacaac agcccaggtt taattttctg tttgaaccgt
     2041 tcaggactga caaacaaaat ttcggagata gtgcatgaca acaagcaaat cactttcacc
     2101 gaccatgcag gaataaaaga gttgcaattc aaaaataaac cgaatgctta ttcaatttta
     2161 gatacatact atggcaaata atttcacaac atccgctaac attctcagag acactgagag
     2221 ggattttaat tacataccga caccaaatgc caagcaagtt gtaagccaag ttgtgaacga
     2281 cttcaagaaa ggaattcgtt cattcaacat aattggaact tatggcacag gaaaatcttc
     2341 attccttgtt gcacttgaac aaagtatcag aggaacgaaa agatattttg atccgaactt
     2401 cattgcccat cctaaagttg acttcgtaaa aattgttggc tcttatactt caatcgtaga
     2461 acaatttgca gacatctttg aagtatcgat aaacaaaaac aagcatgaaa atattctttc
     2521 agaaattttc aatcgttatc actcacttgg aaaaaacaat ggagttctat ttttattcct
     2581 tgacgagttt ggaaaatttt tggagtatgc aagcaagcat aatcctgaaa acgaattgta
     2641 tttcattcag cagttagcgg agttttgcaa caatccgaaa tacaatattg ttctcatcac
     2701 aacggttcac caaagttttg aatcgtatgc atactcactc acaagcacac aaaaacaaga
     2761 gtggacaaag gttaaaggaa gattcagaga aatcactttc aacgaacctg ttgaacaact
     2821 tttgtattta gcagcagagc atgttgcaga aaattttgaa agcaaaccct caaagacagc
     2881 agttgaaaaa tgtttgaagt tggcaacaac aacaaaagct ttcaacttta acaaagattt
     2941 tctaaatgag attgcaacaa aactttttcc gcttgatgtt ctttctgcaa acattctcac
     3001 actttcatta caacgctacg gacagaacga aaggagtttg ttttcattct tagaaagcac
     3061 cgaccacacg ggactttcca aattcaacaa acgggaaaac ccgttttaca atttgagttg
     3121 cgtttatgat tatctcaact tcaatttcta ttcgttcctc acttcaaaat ataatcctga
     3181 cttttctgca tggagttcaa tccgttcatc attagaagaa gttgagagag catttgacag
     3241 cagcataaac gactatgcaa aagcaatcaa ggcaattggt ttactgaata ttttttctgc
     3301 aagcggagca atccttgacg aaaacttttt gagcaactac cttgaaaccg catgtggagt
     3361 tggaaatgca agcagcataa ttgacaatct tgaaaagaaa agaatcattc gtttccgttc
     3421 tcacagcaag cgttacatct tgtttgaagg aactgatttg gatattcaaa ctgcattgat
     3481 tgaagcagga aataaaattt cagaagtcag tgatgttaca accctactta acaaatattt
     3541 tcaattcaca cctgtattgg caaaactcta ttcatacgag aaaggaactc caagattttt
     3601 tgagtttgta atttctgagc atccaattac cagagtaccc gatggagaaa ttgacggctt
     3661 tgtaaatctt gtgttcaaca gcaagatgaa agttgcagaa gttgaagaag caagcagaac
     3721 acagaaagaa gcaatcgtgt attgtttttt caaaaactca acagagataa aatctttgct
     3781 ttttgagatt gagaaaatcc aaaaggtgat tgaagaaaac aaagaagata aagttgccaa
     3841 gagagaattg gaaaacattg ttgaatcaca aattcgtttg ctcaatcact acatcaccga
     3901 tagcatttac acaggaagta aagaagtaaa atggtttttc aaaggggatg aaaagaaaat
     3961 tgccgacaaa aaagatttca ataaacttct ttcgcaagtg tgcagcagcg tgtatgatgc
     4021 aacgcctgta ttcaagaatg agctggtgaa caaacaccgc atatctgcaa gcattcacac
     4081 agcgaaaaga aattatttca aagcacttgc gaataattgg gacaaggaga atttaggttt
     4141 tgaagaagca aagttcccac ccgaaaaaac aatttactta tcgcttctga aggaaaacgg
     4201 catctcacct attcgtgaaa attcgtttaa agttcctact attgacaaac actccagttt
     4261 caataaactt tggaaagcat ccgaaaaatt tattgagaat gctaagttgg agcaaaaaaa
     4321 tatttctgat ttcgtttcac tgttgagcaa gcgacctctc aaactcaaac aaggcttgat
     4381 tgacttttgg attccaacat ttcttttcat caggcgtgat gactttgcca tcttcaatgc
     4441 agacggctac attccaactt tgtcagacga gaatttagaa ttgattgcaa aaaatcctga
     4501 gaactattca atcaaaactt ttgacattga aggtgtgaaa ttggacatct tcaacagtta
     4561 cagaactttc ttgaatcaat ctacagagtt gaagtttgac aacaactctt tcattgaaac
     4621 cataaaaccg tttattgttt tctacaaaca actaccagaa tattccaagc aaacaaagcg
     4681 attgagcaac gctgcattga aaatcagaac agccattgca acaagcaaag accccgaaga
     4741 aactttcttt gaagcgtttc caaatgcctt ggaaatttca cttggcactt tgcaaaaaga
     4801 caaatcaaaa ttgcagagtt acacaacgag tttgcaaaat gcagtaaaag aattacgaac
     4861 ctcttatgat ggtttgataa aacgctttga agaatttatc tgcaatgaat ttgttggaaa
     4921 gaaagttgac tttgaagaat acaaagagca tttgcaacaa cgctttgcca aactgaaaaa
     4981 acatttgctg ttggcaaacc aaaaaacttt tgttcagaga attgattcag cacttgacga
     5041 taaaaaagca tggctcaatt ccattgcaca agcaattaca ggcagaacgc ttgaaatgtc
     5101 aaccgatgaa gatgagattt tactttacga aaaattcaag tcaatgattt tggaactgga
     5161 tagtttgacc aacatttcaa aagccgacat tgacgaaaac aacgaagaag taattggagt
     5221 gaagattgac acattcttta gcaccatcaa tccaaaagtt gtgagagtgc caaagaagaa
     5281 agccaaagaa atagaaatga ttaaaatcgc attgaagaag aaactttcct ctgataaaac
     5341 ttctaacatt gcggctgtat taaacctact aaaagaacta ttgcaatgaa taaagtaagg
     5401 cacgtattag gaatttcggg tggcaaagac agtgctgctt tagccattta tatgaaaaca
     5461 aaatatcctt cattggacat tgagttttat tcatgcgaca cagggaagga actagatgaa
     5521 acctatcagc taattaagaa tcttgaagtt tatcttggaa ttaaaattaa tttgttgcaa
     5581 gctgcacaaa acagttcaga agacccgttt gaccacttct taaaaatgta tggtggcttt
     5641 ttgccttctg caaattcccg ttggtgtacc aagaaattaa aacttgaacc gtttgaaaaa
     5701 tatgtcggtg cagaccttgt gatttcttat gtaggaatca gaggtgacga agaaagagaa
     5761 ggatacattt ctacgaagaa aaacattcaa tcaatttttc ctttcaggaa aaacatttgg
     5821 agtgaagatg ttgttgcaaa acttttctca aaccaaaatt ttgaaaaact tcaaacgctt
     5881 gcaagcaatg ttaacttcgg aagatttcaa gacaaggcaa cacaaatcat tgcaagaaaa
     5941 ttagaaccgt cattcacaca agcacagaaa ttaaatttat tgcttgacac tgatgtgaaa
     6001 ggtttcaatc atttagtatt tgaatttctg aaaacaactg actacccact ttcaaatgaa
     6061 tcatcatttg tattacttga caatgaagat gttttgttga gagccgacat ttttaaaata
     6121 cttgaagata gcggtgttgg agttcctgcc tattacaaca aacttgattt tgatgtgaat
     6181 ggaaaaacag gacaatattc aagaagtcgt tcaggttgct acttttgttt tttccaacag
     6241 aaaattgaat gggtgtggtt gtatgagcag catcccgacc tctacaagaa agcaatgcag
     6301 tatgagaaag acggctacac ttggggacaa acagaaagtt tggaagaact gattcagcca
     6361 gcgagaatgg cacaaatcaa aactgaatac atgaaacgca tggaaaagaa atcacaaagc
     6421 aaatcgccat acctcattga tatattgagt gaagaagaag gtgtaggttg tgcagcttgt
     6481 tttatttaaa aaattaattc taaatcaaaa aatatcatgg caaataaatt tcaaaattgg
     6541 atagaacaat atcctgatta tttggttaat caaaaaacaa aatgggttgt aagagacaac
     6601 agtgttgttg acgataagca gtgggttatt cttgacgata gaggaataat gatttcatgt
     6661 gagtggaaag ataataaaga ggattgcatg gaagaagcag accgtatttg tgttgaggaa
     6721 ttatttgttt aatgaattca ttggaaaaca taatttactt tgacaacaac gccaccactc
     6781 caattgacaa gagagtgttg gatgcaatgc ttccattcct gactacaaat tttgccaacc
     6841 caagcagcac acatcatttt ggaattactg caaacgaagc agtcaaaaaa gcaagaacac
     6901 aagttgctga tttgattggt tcagataaaa acgaaatcat tttcacaagc ggttcaacag
     6961 aagcaatcaa cattgcttta aaaggtattg cggaaaatta ttctgcaaaa ggaaaacaca
     7021 tcataaccgt atcaacagaa cattatgcag tgcttgacac ttgcaagcat cttggaagcc
     7081 gaggatatga agtaacctat ttgccggtga gcaaagacgg cttgattgat ttgcaagaac
     7141 tgaaaaattc tttgcgtgga gacacaattc ttgtatgtgt gatgtatgta aacaacgaaa
     7201 caggtgtaat tcaaccgata aaagaaattg cttcgcttgc acatgaggcg ggtgcattgt
     7261 tcatgacaga tgcaacgcaa gcagcaggta aaattgaaat tgatgttgac accttgggaa
     7321 ttgatttgat gagtatgagt gggcataaaa tgtatgcacc caaaggcgta ggtgcattgt
     7381 atgtaaggca atgcatgaat cgtgtgaaga tacctgcata catacatgga ggcggacacg
     7441 aacaaggtgt acgtagcgga acactgaatg tagcgggcat agtcgcactt gcagaggcgt
     7501 gtgaaattgc acagcaagag atgaaacaag atgctgaaag aatttccgct ttgagaaatg
     7561 aattggaaaa tgagttgctg aaaattgaag gaacatttgt aaacggaaaa acagagagca
     7621 aaattttcaa cacaaccaat atttgttttc gtggcaacga tgcaaatgtt ctaattggaa
     7681 gaatgaaaaa tgttgccgtg tcaaatggtt cggcttgcac atcagcagta gttgaacctt
     7741 ctcatgtttt gacagcaatg ggattgaatg gggatgatgc ttttgcttca attagatttt
     7801 cgttggggag aatgaataca ttgcaagaag tcaaagaagt tcaaactata atttcaagaa
     7861 caataattcc aactttaatc taaataaaat tatcatggca gaagaacaaa ttagattcgt
     7921 aagcgatgca actgctttgc tttcaatgag agattccgat tttgatgctt acagtgcata
     7981 cggagaagtt attgacaatt caattcaagc ggatgcaaca aacattaaaa tcatggtgaa
     8041 gtcatatcat gaagcacgac aaagagttgg cgactatcaa gttgttgact ggattgcttt
     8101 cggtgatgat ggtaatggaa tggatatgaa tactcttcat agatgtttgc aattaggtta
     8161 ttcaagcaga tataatgata ggtcgggtat cggaagattt ggagtaggaa tgacattggg
     8221 tgcaataaac caatgtaaga aagttgaagt ttattccaag accttaaaaa gtaaatggct
     8281 ttacacttac attgatattg aagacatcac aagcagtcct gctaagatga atgcaattcc
     8341 aacaccaaca gagaaagaaa ttccatttca cttggcagaa ttagttacag acaaacaagg
     8401 caccttagtc atttggacaa agtatgataa gcaaccagtt tctgctgatg ccttagaaga
     8461 tgaaatggct gtttggattg gaagaacttt cagaaaattc atttggggag aagtggaagt
     8521 caacggaaaa aagaataaag tcaacattga aatcaacaac aaaaaggttt tggctgttga
     8581 ccccttgcat ttaaagaatg atagatttcc aacggatgtc ccatcttata aaactgaatt
     8641 gatagaattt ccttggcatg ttccatttga agatagaaag accgatgagg aagaaacaag
     8701 tgaaataaaa attcaaatga ctcttattga taaaagtttg agagattatc aaggagtagg
     8761 taatgcaaaa gaaacaaaag atagatacat tgatagaaat gaaggtattt caattttgcg
     8821 aaatggcaga gaagtttttt atggagaaat tcctcattgg aaacctgcct ttgaagaaat
     8881 tgacagatgg tgggggtgcg aaatttcatt tgatgcaata cttgataagt catttgctgt
     8941 taagaatatt aagcgaggtg cagtgccagt tgcaactttg aaacaaacta ttaacgataa
     9001 aatagttccg acaataaaag ttttcagaga agaagtttcg gaacattgga aaagaagaag
     9061 attagagaga acacaggaaa aacttgctca acaaacagaa gacgcaacag caacggggca
     9121 tgaagaagct gaaagtgttg caaagaaatc tgaaacacaa aaaacaaaag ctactgctga
     9181 gacacaggca aatgctcttg agttagcttc tcagattaaa gcaaatgaaa cagcagaaag
     9241 acaagcagct tgggcaacta aatttgccag ccaaccatac acaattattg atgaaagttg
     9301 gaagtcgcct gaatttatag agattcatcc gttaggtgga actgatgtta tcaaatacaa
     9361 tttgaagcat ccattcattg aagagcttta tagtttggtt gagcaaatcc aaaattctga
     9421 aattgaccaa gagttagcaa tgaaacttaa aagcttgatt gaccttttga taatcagtta
     9481 tgcaaaatca gaatcaatgt ttgataagca aaaagaattt aaagcagaaa agttctttga
     9541 agatttaaaa attaactggg gacagtattt aaaaagctac ttagaaactt ggaagtcaga
     9601 gaatatttaa gatgatattt aacaacatac caaagaccca actacaagaa ctcataggtc
     9661 aatctgtttt agacagattg gaaaaaatta ttccagttct taaaggagac gatacagacc
     9721 ctaatgagat ttacaggaag gaaacattgg tgaaaatctt ttcttctttt tcaaattcca
     9781 atttatttcg tgacaaagat tttttcaaag ggttgcttga aacaactcca gtagaaataa
     9841 ttgacgaaat ctgttcggca acaggaattt caattgaggg aaaagttttt gagcaaaagg
     9901 ttgacagcat tttaaaaaag ggctggaagg actttgagtt ctgttcaaaa tttttgaaag
     9961 ttgccgaact ccctgatact tttttaccag ttcaacaaga gccattcatt gcagaagaag
    10021 attgctttcc gcctgtaaca ccttttaaat cattgaagga ttatcaaatt ggagtatatc
    10081 aacaaagtat tgagttggta aaaatcccca attcaagatt cattgttcaa atgcctacag
    10141 gttcgggaaa gacaagaacc tcaatggaga taataacgaa agtgttatgt aaaaatccag
    10201 aaggaagtgt tgttttttgg cttgcacatt cagaagaatt atgtgaacag gcagtgcaat
    10261 gtttcaaaga agtttgggag catgttggaa ataaaaaggt aaagctattt cgtgtttggg
    10321 ccaaaggaaa tttatcattc acatttaacg aatctgcttt catggttggt ggttttcaga
    10381 aactgcatag tctgttgcga aaaaactcaa ctccatttga cgaaattaaa aagcgaattg
    10441 tcttacttgt tattgatgaa gcacataaag ttttagcacc tacatatacg gaagcaaccc
    10501 gtgcattgat gggagagaaa acaaaagtca tcggactgac tgctacacca ggaagacaca
    10561 ttgatgactt agagcagaac aaattattgg ctgatttttt tctcaataaa attatcacga
    10621 taaaaactgc tgacaacaat tctgtgattc agtttttaag ggacaagaaa gttttatcca
    10681 aagctgcatt taaccctctt ataacagaca tcagatatgt tttggacgaa aaacaaaaga
    10741 aacatcttca aaccttttat gatttccctg aagggctttt gaaaaaaatc ggaaatgatg
    10801 atgttagaaa tcttgaaatt gtaaaacgta tcgacagaga gtttacccat agcaaaccac
    10861 aaatgatatt ctttgcgtgt agcattgagc aatcaaagtt tatttgttcg ctgctggttt
    10921 atcttggtta caaagcagca catgttgacg gaggaactga aaaaccaatg cgacaaaaac
    10981 taataagtga ttttaagaat ggtgagatcc aagttatttg taattacggt gtcctctcta
    11041 ctggttttga tgctccaaaa actgatgttg tttttatttc ccgacctaca cggtcaattg
    11101 ttttatacag ccagatgatt ggcagaggac ttagaggtct tgctatcgga ggaacagaaa
    11161 aatgtaaagt aattactgta agagataaca ttatgggact gcctgacgaa gatgacatct
    11221 ttgattactt tgatgaatat tttgagaact gattatgctt tataaaaatg gagttgacct
    11281 gcaacccaaa ctcagtgaat acataagtaa ttcgcaagca ctttttattt tttctccata
    11341 catcaaactt gaaacactca aagcactgat tgacggaaat gaaaatgtaa aagtagtttt
    11401 tgtaagatgg gaaaccaaag atttgattct tggtgcatct gacttggaaa tttatcctta
    11461 cctgaaagca aaaggaatta cgctttatag aaattcacga ctacatttga aagcatattt
    11521 ggatgaatac aaaaattgtt ttcttacaag tgcgaatatt tcttcacggg ctttgaatct
    11581 accactctat tcaaactaca attacgaaat cggaactgtg gttgagaatt tagaaatagc
    11641 agaccgctta tattttcaaa tcattgaaag cgacagcatc ttaatcacag acaacattta
    11701 caaacaactc agcgaacagt tgcctgaaaa gaaaaaagaa tttcctgacg aagtagaatt
    11761 tcatttcaag tttgaagcac ccgacaaaga ttttttgatt tccgctttgc caatgactta
    11821 cagcattgaa acgctttaca gaatttacga ggataaagaa tttgtaaatg acttagaatt
    11881 gaattgcgtg ctacatgact tagctattta caaaattcct ttgagcttac cttcaacaga
    11941 atttagagag aaacttacag aagcattttt ctctcacagg ttcgtgaagg ggtttttaca
    12001 gaacttagaa agcacaggtg aaatttattt tggaacagca aaagattgga ttcataaaaa
    12061 ctgcacagat gttccaactc cacggaaatg ggaaatcaca gagaacattc aaatccttta
    12121 caaatggatt gtaaaacttg gaaacggaag atataaagtt gatgttcccg gaagtcattc
    12181 ggaaagatta agtattgtga tatgaaagca gagaaacctt tgaccaatat agaagccaca
    12241 taaggttcta tattgtttca ccccccctac ctactattcg caacatcttc ttttccggct
    12301 tttacaattt caccatattg cgtttcgtca ccgttaaaag attttagttt tatgcggtag
    12361 tagtttgtgg tgcctgcatc ggttctgtca acaaaggagt agtagtttat ctgattgttg
    12421 cggttggctt ttaccaatcc ggcttcgcta aagtcggttc cgttggcaga gcgctctaca
    12481 ataaaatagt tatggttgtc tgtagtcttg cacttccaca catcacaaat ttcatttaag
    12541 cttttttaac ccttttgcat ttaataaact tttcatttgg gtaatggcaa tttgattgag
    12601 ttggagtagt ctgtcttttt gagccaaacc ctgatgaatt aaaatagcat taatactttc
    12661 gagattcgat aacactacta attgctcaat tgtggcatgg tcacggatat ttccttcaag
    12721 cgtaggattt gaatctctcc attctttcga agtgatacca aacaatgcta cattaagcaa
    12781 atcagcttct gtggcgtagg taaagcttgc ttgtttgctg ctgatgctac caggaataag
    12841 tttttctttg atggcatctg tatgtatatg atagttaacc ttggctaaag tgcgttgcaa
    12901 gttccattct aatttgagac ggttgttttc atcttctttg aggcgttgga attctttaat
    12961 taggtaaagt ttgaattcaa tagatatcca tgatgcaaat tcaaaggcta tatctttgtg
    13021 agcaaaagtc cctccatatc taccggattt ggaaataata cctattgcac ctgttttttc
    13081 aatccactgc tttgaagtta aaacaaaact gtttaaccct gcttgttttc taaacccctc
    13141 gaattcgatg ggattaaaat ttgggttatt tagctgttcc caaaatccta atagctcaat
    13201 tgtatttcta ttccttaacc aattttggat gatataatct gttctttcac tgtctttgtg
    13261 tcttgccaaa tcagtcagtg agataaagtc atcttgattt aaatggtaaa gagaaatttc
    13321 aattcctttt acttctagtt tttattggtt gacatacctt gcagttataa aacatagttc
    13381 agttcttaaa acaactcaat atattcttca ttgaataatt cattagaaca atttaatcca
    13441 tggtattaaa cataattaaa aaatgaaata aaccacaggt gcacttaata aaatttggaa
    13501 agactttatt taatttccct gcaaaaacct acccccccct acctactatt cgcaacatct
    13561 tcttttccgg cttttactat ttcgccatat tgcatttcgt caccgttaaa agattttagt
    13621 tttatgcggt agtagtttgt tgtgcctgca tcggttctgt caacaaaaga atagtagttt
    13681 atctgattgt tgcggttggc ttttaccaat ccggcttcgc taaagtctgt tccgttggca
    13741 gagcgttcta caataaaata gttatggttg tctgtagtct tgcacttcca cacaacacct
    13801 gatgtgtttg catgttgaat aactttcaat tcagggtcat catctttaga gatggcaatg
    13861 atagaaacgg cattgtctgt aagcctccag gcgcggtcaa ctcccaacga tttgagtaca
    13921 atatttactt tcagaacggg cgatttttct ccttcaactt cgtatttaaa ttttttgctt
    13981 tctgtcatat cgctaccctt aaccacaaac gattttgcta tggcctcgtt ttgcataatg
    14041 taaataatac attggtcatc ggcattgagt tttccatctg ccgttaaggt tacttctacc
    14101 tgatagcgat agttgccttt ttcattacac agtctgttgt ttgatgtaaa tatggcagag
    14161 tcgttgcgca cttcccaata ttctgagttg ataacaccac cggaaacttt aaacgtttct
    14221 gcttttttaa aatctactga atagattgtt ttgtcgcctg cctttacaaa catgggcgcg
    14281 aatatgataa acagaaaaat gtatctgata cttttcatcc ggttgattat ttaatcatac
    14341 aaatattgaa gaaatgagca gtctctttat gctacttttg ccttactttt caaatgtcag
    14401 gtgtttatat ccatattcct ttttgcagca aggcttgcac ctattgcgac tttcattttt
    14461 cgacacagct acgaaacaaa aacgcattta ttgaagccct gttgcatgag atagaagtgc
    14521 aggcacgcta tattgatgag gcagttaata caatctattt tggtggagga acaccatcat
    14581 tactttcagc agcagagatt aacagtatta tcggaaagct agataaagtg tttaaacttg
    14641 atgttaaaga aataacttta gaagccaacc ccgataatct ttctgaaaaa tatctgaaag
    14701 aattgaagtc aacagcagtg aacagactga gcattggtgt gcagtcgttt gaagaaaaga
    14761 ggctgctgtg gatgaacaga agtcatacta caaaacaggc aatatttgct attgagtctg
    14821 cactgcaaat gggatttaat gttagtgccg atttaatttt tgccttgcct gaaatgtcaa
    14881 tagatgaatg gcaaagacag ttgcagaaga tgataagttt aaaaccacat catatttctg
    14941 cctatagttt aacacttgaa gaaagaacgg tttatcatca tcaggttgcg aagaaaaaag
    15001 tcaaggagtt agatgatgtt aattcggaag agcagttttt gcaggcacat caactgttga
    15061 gcaatgcagg gtatcatcac tatgaaatat cgaactacgc aagtgccgga cacgttgcct
    15121 tacacaacag cagctattgg aacggtaaac cctatttagg tctaggacca tcggctcata
    15181 gttttaacgg aaaaatgcgg caatggaatg tcagcaacaa cactgcgtat attaaagcat
    15241 tgagtgcagg aaaactttct tttgaaagtg aagtgctgac agaggttgac cgatacaatg
    15301 aatatgtgat gacaggcctg agaacagata aaggcattga agaaaaacaa tggaatgaat
    15361 taatgccgtc attgtataaa gagagtttgc agaaagaaat gaatgagttg gttaatcagc
    15421 aatttttagt tcgtgatgct tctcgtatat ttataccctt gcagcattgg tataaagcag
    15481 atcagttaat cagcaggttg tttgcaacag aaaatagtta aaagaaatga ttgcacattt
    15541 aaaaatagga gacgaagagt attcagctaa gctcaatgag ccaatagata tttctattcc
    15601 attgcactcc ggcaatgata atgttaatgc atggtatgca cctccaccgg aaattgttcc
    15661 tgtgcgcatg gatagttggg taggcgaagt ggcgcaaggt gcatcagtaa atttcaggaa
    15721 tgtgtttttt aaccctcatg cacatggtac acatacagag tgtgtaggac atatcaccaa
    15781 ggagtgggtt tcgttaaata aagtattgaa acaatttttc ttttctgcaa agctgataac
    15841 ggtgttgcca atgattgttg atggcgatat gattattacc aaggaagatg tagttcaggc
    15901 atggaaaaac tgtggtgccg aagcattagt cattcgtaca ttacctaatg acaacagcaa
    15961 acaaagcaga cattattcaa ataccaatcc gccctatctg catcatgaag cagcagcgtg
    16021 gatggcagaa aagaatatca aacatctgct actcgacctt ccctctgttg accgcgaaca
    16081 ggatggagga aagctgcttg cacacaatgc attttggagt acaggaaaaa atattcgtta
    16141 tgattgcact atttcggaaa tgatttttgt gcccgaccat attgccgatg gactatattt
    16201 gctcaatatt cagattactg cattggagaa tgatgcaagc ccttccaaac cactattgtt
    16261 tcagttgact aaaaaataaa atgtgtagag atgaaaaaac tgcttaatta tttctttcag
    16321 ggattgttgg ttgttgtacc tattgctgtt accattttag taatctataa aatcataacc
    16381 tggattgaca acctgttgcc ttttcagatt ccggttaagt tgcccggcat tggcgaagtg
    16441 agcattcccg gacttggcat tcttaccatt gtagttgctg taactgcttt tggttactta
    16501 gctaaatttt ttgttgctaa tccttttttt gttttgctcg aacgcatgtt ggagcgaaca
    16561 ccgttgttaa aaattattta cacttcagta aaagatctga tagaagcatt tgttggagag
    16621 aaaaaacgat ttaatcagcc tgtgttggta acggtaaata aagtgtcggg agtgcagcgc
    16681 attggtttca tcacacaaaa agatttatcg gtgcttggca tcagcagtaa taaaatggct
    16741 gtttatcttc ctttctccta tggttttaac gggcagttgg tgatagtgcc tgcagagaat
    16801 gtagagaaga ttgatgcatc aggaacagag atgatgaagt ttgtaatttc aggaggtgtt
    16861 acagaagtag attaaacaaa gaactatgaa tatttttcgg tttgcattat ttgcagcaat
    16921 gttaatttta atggggtcag gtgtttgtgc acaggattat tttattccat taaatagaga
    16981 gtggaacctg cgctatgagc cttttcttca gacaacaaaa agcaataatg ttcacacttc
    17041 aataaagcca tacagacatg atgaaattat tgctgttgca aacattgatt ctgttgacac
    17101 agcaaatttc ccgataagtc gtttttcaaa aaccttagtc ggacgaaaaa tttttaaaga
    17161 gcatttagta caaatcaagg atgatgactt tctgatacat gccgatttta tttttgaagg
    17221 acgcattggc aaagaccttc cggaaacaga aacattattc actaattcaa gaggattctg
    17281 gcttggcgga acggcaggaa aaagattttc tttctctact acatttttcg aaaatcaggc
    17341 aaggtttccg tcatacattg atacgtttgt tacacgctac aacattattc ccggtgaggg
    17401 gcgcataaaa aaacaatatg gcagctatga ttattccaac gttacaggaa ctatatcata
    17461 caccatcaac agacatttta atgtgcagtt tggacacgat aaaaatttta ttggcgatgg
    17521 ttatcgctct ttgcttttgt ctgataatgc caacagctat ccttttctga aaatttcaac
    17581 tacggcatgg aaggttaagt atgtaaattt gtttaccgta ctcaccgacc ttcagtttcc
    17641 tgcacctaat gacattgctt ttaaaaagaa atatgcatca ttccattatt tagaccttaa
    17701 catcggcaaa catgcatcgt tcggaatttt agaaagtgtt gtctggtcgg gcgactcggc
    17761 acgtgggcag ggttttgaca ttaactacat gaatcctttt gtatttattc gtcctgtaga
    17821 gttttcaata ggttcgccag ataatatgat gctgggtttt aatctgaaag taaagttgtt
    17881 ttcgaaattg gtattttacg gacagttgat gttggatgaa tttaaactat cggaaataag
    17941 agacggcaat ggatggtatg ctaacaagca agcatttcag ttaggattaa agagttatga
    18001 tttgccgatt aaaaatcttg atgtgctgac agagtttaac tatgtaaggc cgtttactta
    18061 ttcgcaccgt acaaccttta caaactatgc acattttaat cagtcgctgg cacatccttt
    18121 aggatcaaac ttttatgagt cgatgtcttt gattaactac aagtataaaa attttatttt
    18181 ccgttttcag tttaactatg ctgagactgc aagcgataca ggaaatgcaa atttgggcaa
    18241 cgacattttc aagagctacg aaacctacac ttttgcttat ggtaacagaa tgcttcaggg
    18301 gcaacgtcac aacctgattt atagcgacat cagagcagga tacttaatta acccacgtac
    18361 caacttatgt tttgagattg gttatagcta taggagaatg acaaccaata gtgaagcagc
    18421 caccacaaac tgggtttatg caggtatcag gacagcactt tttaaccgtt atcatgattt
    18481 ttaattacac ctatatttac agttgtttta cctgaaaaaa actgctattg gcaaaatagt
    18541 acctttgtag taaatattga gagatacatt tgagccaaac agattacgca ccatgattaa
    18601 tattgctttt agttgtatta ccgcttttat tattaccttt cttgccatac cgtcaattat
    18661 taaggttgcc gtaataaaaa atctttttga tgaaccgggc ggacgtaagg cacataaggc
    18721 aagtgttcca acccttggtg gcttagccat ttttgccggt gttgtttttg catttacttt
    18781 ctggtctgcg gggtttaacc ttcaggacac acagtacatt attgcagctt tgatagtgat
    18841 gttttttatc ggaataaaag atgacattgt tgaacttcct gcaatgaaaa aactttacgg
    18901 acagatagtt gcagctgtta ttatagtctt gtttgccaac atccgcatta caggtcttta
    18961 tggaattttt ggaatagatc aggtgccata ttggttcagc cttttgtttt caatgtttac
    19021 cattctgctt atcatcaatg cttttaactt gattgatggt gttgacggac tggcagcagg
    19081 cattggtgct attgcagctt ttacttatgg aatttatttc tacaactata atgagatagc
    19141 tctatgtatt ctttcgtttt cattattcag ttcgttatta gctttcttgg tttataattt
    19201 tgcacctgcc aacattttta tgggcgatac gggttcttta atcattgggt tgattttatc
    19261 tatcctcact atcaagttta ttgaaatgag ttttgcacca cgtccggaaa actttccatt
    19321 tcagagtagt ccggctatgg cagttgccat tttggtgttg cctttgtttg atttgctcag
    19381 ggtatttatt gtcagaattt taaacaagcg ttcacctttt caggcagaca gacttcatat
    19441 ccatcacctg ttgctcgata tgggtttaaa tcaccgcgaa gtttccatta cactttactt
    19501 ggtgaacata ggctttatac ttgtggcatt ggcattacgc agctttggcg cattggcatt
    19561 gttgtttgtt attagctctt tagccttgat tttgagcttg gtgccctact acttaaagta
    19621 tcaggcaggc cgttcagggg cagtttcaag gtcaaccaag tagggcatta ataccctatt
    19681 tgcctcactt acatgaaaaa ttgatataat tatgtaagca tttccccaaa aagtaaacat
    19741 tttttttatt ttcgtccgaa cttgaataac aggcactttt ccggactctc tgcctgctaa
    19801 attaattatc atgcgactaa aaaaactggt ttactccatt gctcttttat tgccgttcat
    19861 aggtaatgct caggtagttg aaaatgcaca acctcaatcc ggtgacccta tagctgccat
    19921 gttggacagt ttggtgtcta ttcgtaatgt tgtgcgactg agcgaaacaa accttcagca
    19981 gcctcctgtt tttagtgaga acgatgctgt gctttctgat gcttttattc aggaaaggat
    20041 ttcaaaaatt tcgacaccta taccgttaac ctataattat caggttaaaa actacataaa
    20101 tctttatgcc tcacaacggg cacaactgac ttcacgtgta atgggtttat cgcgtttgta
    20161 ctttccgtat tttgaacaaa tgctggacaa gtacaatctt cctgttgaat taaaatatct
    20221 ggcaattgtt gaatcggcac ttaacccgac agcagtatcg tcagcaggtg caacaggtat
    20281 ttggcagttt atgttcaaca ccggaaaaat gtatggcctg aacattaact cctacattga
    20341 tgagcgcaga gatgtgcgtt tggcaacaga tgcagcatgt cgctattttc aggatatgta
    20401 tgctatttat ggcgattggt tgctggtgat tgcatcgtac aactgtgggc cgggaaatgt
    20461 gaatcgtgct attgcgcgtg caggcggcag caaaaacttt tgggagataa gtcgttttct
    20521 tcctgcagaa acaagaggtt atgtaccggc ttttattggt gctacttatg tgatgtatta
    20581 tgctaaagat tataacatca cgcccacaaa accttcattc acttattttg atgtggacac
    20641 tgttggtgta aaaaataaaa tttcgtttcg tcagttgagt cagacgctaa acatacctta
    20701 tgatgtgatt gcatttttga atcctgttta caaacgcaac atgattcccg gtgagggcaa
    20761 cagactttat ctgccaacag aaaagataag tatgtacctg gctaatgaag aaggtcttta
    20821 tgctgcaagt ggcacagagt tgcaaccatc atcgtttgct attgctgatg atgttggtta
    20881 tgtgcaggtg aaacaacatg taaaagttcg taagggcgaa aatctcttca cctttgcaaa
    20941 aagaaatgat tgctctgcca aacaagtaaa gaagtggaat ggtttaaggt cgaacaatat
    21001 taaagcagga cagaaattag ttgtctatgc aacaataaag caaaaacctg caccggttga
    21061 gaataccact gcaactgctg caaatgataa aacagactcg gctaatgtaa atacaacagt
    21121 taaagcagat tcatcatctg actctgaaac aggtgctgtt acaactgaga gcaaaacaac
    21181 tcaggtaagc caggcacaaa gtgctgcatc aaaatacatt attcatttag tgcagccggg
    21241 tgatacactt tggaatattg caaaacgcta tgaaggtgtt actgtagagc tattgaaaga
    21301 ggcaaataat tttacaccta aatcaacact taaagtagga agtaaaataa aagtccctac
    21361 agagagttaa ttttcaattt cggttttttt aatggttgtt gtcttcaata tgctgacgga
    21421 attcgttttt aatatcctgc agtgcaggtg tgaggaatgt tgaggttttt aggacaggat
    21481 catagagttt agattctttt tttgtctgac tattgataaa gttgagatgt ggttcgagag
    21541 atttgagcag ccacaggaac acactcagca ccaaagcata tttaagcaaa ccaaatacac
    21601 caccaataat tttattgaaa agattgaggt cgcctgcttt gagaatacct tcgagcagac
    21661 gggcataaag aataaacact gccataatga ttccgctaat aataaagaag cctacccatg
    21721 caggcagggc actgtctttg tttactaatt gaaagagcca gccttgcaca acgcctgaaa
    21781 atttgaatgc aacatagatt ccggcaatga gaccgagtat catggcaatc tcataaataa
    21841 tgccgcgagt gaaacctttg tatgctcccc ataataaggg cacggctaaa aacaaatcga
    21901 gaatattcat gctatggaat agtatgctca aagtaagcga tgctgttgta atctacaaac
    21961 agcatcagca gaaaatacca aacaataaat tgttgaaagg tgttggttaa accaatgtaa
    22021 tatcaaactg aaccagatca ataaactcct gaatgcgttc gttgagttcg tcactatcga
    22081 ggttcatgat tctttctgtg ccaaattttt ctacacagaa agatgccatt gcagaaccgt
    22141 agatgatggc acgtttcatg ctgctgaaag agatgtcgcg ggtttttgca agatagccaa
    22201 tgaagccacc ggcaaaagtg tcgccagcac ctgtggggtc gaacacatct tctaatggca
    22261 gtgcaggagc aaaaaatact tcgttgttgt ggaagaggag tgcgccatgt tcgccttttt
    22321 tgatgatgag atattttggt cccatgttta gaattttctt ggcagctttt accaaagaat
    22381 attcgttggt gagttgtcgt gcttcggcat cgttgatggt gagcacatca atcattgaaa
    22441 tgtttttgag cagatcgtcc atggccatat ccatccagaa gttcatggtg tccatgacga
    22501 tgagttttgg acgtttgcgc aaacgctcta ttacctgcaa ctgaattttg ggcatgaggt
    22561 taccgagcat gaggaattcg cagtcctgat aactttcggg aataatgggg tcgaagttgg
    22621 caaggacgtt gagttgtgta tctaaggtgt cgcggctgtt catgtctgta tgatagcggc
    22681 cactccagaa gaaagatttt tcgccttcga taatctgcag accttctgtg cgtatgttgt
    22741 ggttgttaaa gtcctgaatg gtgctttgcg gaaagtcatc gccaacaacg ccaacaagat
    22801 ttattttagg agcaaagtag gaagctgcaa gtgaggcata ggtggcagca ccaccaatta
    22861 ttttatcggt ttttccgaag ggtgtttcta ttgcatcgaa ggcaacggtt ccaacaacga
    22921 gtaatgacat gatgtgagtt tgaaaatttt gcgcaaagct aatactatta tcggtttttg
    22981 tgattgtgtt ttgtaaatga gttgttgcgg caatgcaatt acacgagcta atatttcttc
    23041 attaaaaaaa taaggagata gatgtatctt taaaccttaa tcttttaata cataaaaatc
    23101 atgttagaac aattattaaa tcttgtaaaa gaaaatgccg gagaggctat cattaacaat
    23161 caggctattc cgaatgaaca gaatgatgca gctattagtc aggctacgga aggcattata
    23221 ggcggattga aatcgcaatt ggcatcgggc aacattagtg aagtagcaca atttttaggc
    23281 ggacagaata atgcagcaac aacgggtgtt acgaatgcca tttcatcgca ggtgtcgcag
    23341 gggttgatgg aaaaatttgg tttgtcggga aacagtgcag gttctatagt gtcgtcactg
    23401 atacctgttg tgttgagtaa gttggtttct aaaacgaatg atcctaacga taagagtttt
    23461 gatttgaata gtatttttaa tcatttgagt ggtggcaaaa cacagggaat ggatttgtcg
    23521 aacatattgg gttctgtgac tggtggcaat gcaagcggag gcggaatagg taatgtgctt
    23581 ggctctgtga tgggtggaga taaaaattct gcactaggtg gactgatgga tatttttaag
    23641 aagtaaggaa aaacacctta gtatcaacat taattattaa tcccttcggt tttatcaaat
    23701 tgattttact gaagggattt tttaattcta aatttttaaa tctacaccca aatatttcaa
    23761 ctttcaaaca ttccaacttt caaaactcaa atctttaaat gcaaacgtcc gatggcaatg
    23821 gtcattctgc tgatgcaaac gggttgctgt ttttcgttta gaatttctat tgtccagact
    23881 tgtgtttttt ttccaaggtg gaagggtttt gcagtaccat agacgaagcc ttctttaaca
    23941 ggtcgcacgt ggttagcatt tatttccata cccacacagg ttgcgctttt gtctttgagt
    24001 gtgagaaatg cacccaagct tgccagcgac tctgcaagaa cacatgatgc accaccatgc
    24061 atgatacctt ttggttgccg tgtgcggttg tcaacaggca ttttgccttt tatagtgtta
    24121 ttttttatgg aggtgatttc gatgcctatg tggttgagca ttgtgttttt tcccatcagt
    24181 tttacttcct ccagtgtata actgccaaaa attgaatcca tattttattt ttattttttt
    24241 tgtttaggtg gaaaaaccgt gcggtagatt tcgtccacaa aaattttaag tcgcagaatg
    24301 tatattggtg atgatagatt gtcttttagc tgatggttgt ttttgagcat gacacgatag
    24361 cccacgccaa agcccaggcc cacccaacgg atgagtttat agtgtccggt gatggcaggc
    24421 tcgaagagaa tgatgttgcc ctgcaaagct ttgttgtttg ctttttttcc gtcaggatat
    24481 tcgtagtagc ttgtgccgaa gccaaagtgc atgggtacgt tgacctgcca ggggccgttg
    24541 cggtagatta catattcaat gcctgttgta aaataattac ttcgcacttc gccacgatag
    24601 tatttatttg gttttacttg aacgctgtca accacatcag taaacagttt catgtagccc
    24661 atgctgaatt ttacacgttt gccgaaatct aaacctgttt tgaaaccgat ggcattggca
    24721 ccgcggctgc gaacgaagga gttgtagccg tctaactgaa aaaaaggttt tggtctttgt
    24781 ttaaataaat aggccaacgt gtcgcgtagt tgtgcatgca cgttgccact gcatgataac
    24841 agaacgaata tggtgagcag cgtgtatttt atttttttgg aagacatggt tgagccgaca
    24901 gaacaaaaat ataaaggtgt gttgttttat ttctgaaata ttaatttgct tactaacaat
    24961 tgcaattgaa ttacggaact gatgcaggtc agtgcgtatg aaggcagaag tgtgtttatc
    25021 ttttataact ttgcacaaag agtgtttgtc aggatttata aaaatgctga gttttaaaaa
    25081 agtaaataat gaaagtagag attaaagttc caagtccggg tgagagcatt acggaagtac
    25141 agattgcacg ctggataaaa aaagatggcg attatgttga gcgcgatgaa gagattgcag
    25201 aaatagattc ggacaaggca acgctgtcgt tagttgccga agctgatggt gttttgaaaa
    25261 cccttgttgg cgaaggagag aatattgcag ttggctctgt tgtgtgtacg atagatacag
    25321 atgcaaagcc ggttgcaaaa gcagaaccta agaaagaaga aaagccaaag gaagaaaaac
    25381 caaaagaggt aaaacctact gtgacagttg catcagcaaa agcagaaggc tatgctgcag
    25441 gcacaccgtc tgttgctgct gctaagatga tgaaagaaaa taatgtgaaa agtgaaagtg
    25501 tgcaggcaac aggtaagggc ggacgtatta caaaaggaga tgtgttgcag tcgttacaaa
    25561 cacctgctgc cacacaagcg gcaccacagg cacgtgaaca acgcagagaa aaaatgacaa
    25621 tgctgcgtaa aaaactttcg cagcgtttgg ttgcagtaaa acaggaaacg gcaatgctta
    25681 caacttttaa cgaggttgat atgagtgcca ttatggacat cagaaaaaaa tataaagatg
    25741 tttttaaaga aaagcatgga gtaggtttag gctttatgtc gttttttacg aaagctgttt
    25801 gcgaggcgtt aaaatctttt cctgcagtga atgcacaaat agacggagaa gaaattttgt
    25861 atcacaacta ttgcgatgtg ggtattgcag tgagtgcgcc aaaaggattg atggttccga
    25921 ttatccgcag tgcagaaact ttgtcgcttg cacagataga acaacaggtg atagaccttg
    25981 ctaccaaagc ccgcgacagt aagctgacga tagatgaaat gacgggagga acttttacga
    26041 taactaatgg tggtgtgttt ggttcaatgc tttcaacacc aattattaat ccgccacaga
    26101 gtgccatttt aggaatgcat aatattgttg agcgtcctgt tgcagtgaat ggagaggtag
    26161 ttattcgtcc tgtaatgtat gttgcattat cttatgatca tagaataatt gatggcagag
    26221 agtctgttgg atttttagtt aaagtgaagc agatgcttga agagcctgca aaaatgttgt
    26281 ttgaaggcaa cgacccaatt aaagtattac tacatttgta accacttttc ttttaccaat
    26341 aaacaaaaag aggctgcctg aatcaggcag cctctgctat tttattttgt gatgtagtta
    26401 ctccacaact ttcatttcgt caacaatgtg tttggctcca ccaaatttgt caatgcaaaa
    26461 caaaacatat ctgatgtcaa cacaaatagt gcgtttgtat tcttcgtcat atttaatatc
    26521 gccactcata gcttcccagt tgccatcgaa tgcaagtccg ataatttcgc cattaccatt
    26581 gataacagga cttccgctgt tgccacctgt aatgtcgttg tttgtgagga agcaggtgtt
    26641 taatgttccg ttgctgccat agcgaccgta gtcttttttg ttatacagct caactaactt
    26701 ttctggaact ttaaattctg tattgctgtt atccattttc tgcatcacac cttcaatggt
    26761 tgtaaagtag ctgtacttaa cagcatcggc aggatcgtaa gcacgcacag agccatagct
    26821 taaacgcatg gtgctgttgg catcgggata gagtttttta tcaggcatca tttcgcgaag
    26881 gccttcaata tacaatctgt tgttggcagc taaggctaaa tcaatggtgc ggataggagc
    26941 acggaagttg tcatcataaa aactcaactg tgtgtttatg agtttaaatg ctggatcgtt
    27001 tttaagtttc tttgctaaag gattttctaa gaatgcaagc actttgtttt tgtcggagaa
    27061 aatacttttt gcaaaaactg cagcagccat tttgttgtag tcgcctttat atttcttagc
    27121 catttctgca aagatgggtg actgctgatc ttttgcaacg tcatcggcaa acgattttac
    27181 aagaacggca aaaacttttt ggtcaacacc ggcatcgtac tcttcaaaat aatcttctac
    27241 tcttgattta agcattgctg cttttgcttt tatatcatcg gcatttggtt ccttctcgtt
    27301 aagcgctttg tctaaagcag aaaacgaaaa tgcataacgc aaaatttctg ttcccattaa
    27361 tgcctctgaa gtataaacac gcagcttggc atattttctg gcattgtcat aagattgctg
    27421 aaggttgccc atcacaaatc catagtgcag tcttcttttt ttatcggcat taacccatgt
    27481 agtgaattga ttttcgaggt tgcgtttttg ttcaacaact ttcat
//
LOCUS       DAOOOI010000021        27429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_309_length_27429_cov_24.020384, whole genome shotgun sequence.
ACCESSION   DAOOOI010000021 DAOOOI010000000
VERSION     DAOOOI010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 27429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27429
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_309_length_27429_cov_24.020384"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..1819
                     /locus_tag="PKX59_04555"
     CDS             266..1819
                     /locus_tag="PKX59_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOZ90171.1"
                     /translation="MSNDLVIDSSPSEVRIALLEEKKLVELNSEKKDRSFAVGDIYLG
                     KVKKLMPSLNAAFVDIGYEKDAFLHYLDLGPQFQSLLKFVKDTQSGRQTNATLNNFEL
                     EKDIIKTGKITQVMSSGLWLPVQIAKEPISTKGPRITTEISIAGRYFVVSPYYNHVSV
                     SSKIKSPEERNRLKRLALSIKPKNFGIIVRTVAEGKKVAELDKDIQDVLMRWNLFYDT
                     LKVAQPGQKVLGEIDRTSSILRDMLNPNFNHIHVNDNLLYDEIKSYLRSIAPEQEEIV
                     KLFKNKQPIFEHFGIDKQIKSDFGKTVAMQNSAYLVIEHTEAMHVIDVNSGGRNRGDS
                     DSQEKNAFETNCMAAKEIARQLRLRDMGGIIVVDFIDMINLEHKRQLHEVLKEEMKKD
                     PARHTVLPPSKFGLIQLTRERVRPEMKISTTEKCPSCNGTGEIKASILLIDEIENNVR
                     YFAHEQNEKKIILHVHPYIEAYLNKGLISIKWKWYLRHKIKVSIEAVSSFAVTQFAFY
                     NTKGEEIKI"
     gene            1920..>2209
                     /locus_tag="PKX59_04560"
     CDS             1920..>2209
                     /locus_tag="PKX59_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011622930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90172.1"
                     /translation="MRKYFILLVVVFCMDSTNLFAGKESSVTDKRFDSFKERFIKDLW
                     KHNPMWATEIGFHAYDSILEINNEQYRNGQVAFSKSYLDSLKTFDKVALSSL"
     assembly_gap    2210..2296
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2297..3684
                     /locus_tag="PKX59_04565"
     CDS             <2297..3684
                     /locus_tag="PKX59_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HOZ90173.1"
                     /translation="WQWNPSSYNATGNVAFMLSENYEPLNKRLVNIGKKLSQFKSYYE
                     VAKKNIANPTDEHLKLAMEQNAGGIAALETDLLDSLKQSTLKADVKLNITQSLTDAKK
                     AVEDYIAFLKDFKNENPRSFRLGKDLYAKKFEYEIQSQYTYQQIYDSAMQRKSYLHNE
                     MFKISDKLWSKYFGTKAKPVDSLAMIKMMIDTISVRHVKPDEFQSAIEKQLPELVKFI
                     NEKNLIYIDPSKPLKVRREPAYMAGVAGASISSPGPYDKGGNTYYNVGSLSGWSAERA
                     ESYLREYNHYILQILNIHEAIPGHYTQLVYSNNAPSIIKAVFGNGAMIEGWAVYGELM
                     MLENGYGDNEPEMWLMYYKWNLRTVCNTILDISVHTKDMTEAQALNLLENEAFQQKAE
                     AQGKWRRVSVTNVQLTSYYTGFKEIYDLREELKKKMGSKFNLKAFHEKFLSYGSAPVK
                     MIREEMLSEIK"
     gene            3686..5716
                     /locus_tag="PKX59_04570"
     CDS             3686..5716
                     /locus_tag="PKX59_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOZ90174.1"
                     /translation="MIRFERFLTLIKRQFYIYVGGLFLLLSIRLFFLFRLFSIAEIKL
                     NVVDILHALAVGFRFDTMVLMYGMALPVLLSILSLIVGTAKSKDMVMRFNRVYAIVML
                     MLFVIVGIVDVYYYNYFQSHINVLAFGLFDDDTAAVLKSVWTDYPIMSVIAAWIIVFF
                     LIRLFVNYVFSDKVKPVVINKVWQGAVCSLLALAMFFLGMRGSIGTFPLQIDDSTVCN
                     NSTINLIPINGVFAIKEAFLMRSKQMSIEGNITFLKKLGYGSEVKAIEDYYGHAPEKD
                     LNKSYFTVTPNKPSGFKHPNVVFFLMESMSNNNLFYHSATLNLYGALEKHLSTDYFLR
                     NFLPSGNGTINSLEGIMVNTPVTSLAQSEHNSVSYSSSVALPFRNSGYETTFITGGKF
                     GWRNINEFIPHQYFQHVESKDNVLKEIAGSTECEWGVYDEYLFDYVFKKLEKATKPQM
                     IFVLTTTNHTPFHLPETYRPYPIKLTNEINSKLLTDENLAVKNLTNLQYSNDCLGKFM
                     DKVKHSNLSQNTIVAATGDHNNLMLFDFNESQMYYQRSVPLYMYVPETIHPHVAINKW
                     GSHKDIFPTLINLALPQSTYFNAGDNLFDTLQSRNNIFATDWMSMEAMNEFGAVKYGE
                     SVKYFDWDKNKMLKETATPSADLQKLLQRARAHYAAMAYFIRSETLKTKQGK"
     gene            complement(5720..5962)
                     /locus_tag="PKX59_04575"
     CDS             complement(5720..5962)
                     /locus_tag="PKX59_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90175.1"
                     /translation="MEDFIGFIGVFILLVAYFLHLFRIIKPDKLFYLILNFIGASLAC
                     LASVLIHYMPFIILEAAWALVSLISIIKLFLLKPTN"
     gene            6041..7180
                     /locus_tag="PKX59_04580"
     CDS             6041..7180
                     /locus_tag="PKX59_04580"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HOZ90176.1"
                     /translation="MKFATKALHAGVEPDPTTGAIMTPIYQTSTYVQASPGQHKGYAY
                     ARGKNPTRSQLEKNIAALENAKYGLCFSSGMGATDAVMKLLKPGDEVISGDDLYGGTY
                     RMFTKVFANYGIKFHFINLTDANNISKYINSNTKLIWVETPTNPTMQIVDIKACSAIA
                     KKNNCILAVDNTFASPYLQNPMDLGADIVMHSVTKYLGGHSDVVMGALCMSDDSLYER
                     LAFIHNSCGATPGPMDSFLVLRGIKTLHLRMERHCFNGKQIAAFLRAHPKIEKVYWPG
                     FEDHPNHHIAKMQMRDFGGMISFDLKSHSQEEAFKLASSVKVFSLAESLGGVESLVNH
                     PASMTHASVPVAERVRVGVTDSLLRLSVGVEDIDDLIADLKQAIG"
     gene            7265..9358
                     /locus_tag="PKX59_04585"
     CDS             7265..9358
                     /locus_tag="PKX59_04585"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HOZ90177.1"
                     /translation="MLKKSTNFLLLMMAMIATITVNAQNTHWCATDEVTNAALQNSAA
                     ARAAQNQLEAFTQQYVQNFQPSYFRSTGTAVYVIPVVFHVIHNWGPENISDAQVYDAI
                     RVLNRDYNGQRTDTANIIPDFKALNADVQIEFRLAHKDPNGNCTNGIDRQQSLLTYDA
                     GDQSKLNPWPYNKYLNIWTISNFGSSHAQAAAYAYKPGTAPPGKDGIIALHNYVGSIG
                     TGSSTGQYTLTHEIGHWLNLNHTWGGTNQPGVACGDDNVTDTPVTKGYTTCPSASNAD
                     VCNPGVIENYQNYMDYSYCYYMFTLGQKARMWAALNSGAGLRNQVHTATNLTNTGTDG
                     GTYVCAPTASFSPVQKVICEGGTVSFSDASLNTDTVGTTYLWTFNGGTPSTSTDKNPT
                     GIIYNTAGIYDVTLTVTTSAGFDTYTGIESVVVRPQQVSNFVPYSEGFENVLFPSVDW
                     IVDNENGNAWELNSTAAHSGFNSVRINNFSGNVAGTKDVFITPGFNLTNTTQTQFTFW
                     LAYALKNSSGTDQLKVYASNNCGQLWNLRTTKTGTQLATVSSTVTSDFVPNAADWRQE
                     TVSLTSGQYSTKPNVSLKFEYTHDKGNNIYIDDINLTGVVGISDYAVSASDVTIVPNP
                     ASEKSSVNFELLQPEKVLITVTDMLGKEVHRISENRLDAGEYQYEVNTNWAKGVYTVR
                     IAAGSQIISKKLVVE"
     gene            9422..9832
                     /locus_tag="PKX59_04590"
     CDS             9422..9832
                     /locus_tag="PKX59_04590"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOZ90178.1"
                     /translation="MSESKFSIEMNYVVYAKPEAVFNALTNPDVMKKWIEGSFVFELK
                     KGGNVELFDGWVKGEVITFINNESFSYTWKPNTWDKKTKPSLVEIELKPDAAGTRVYI
                     HHSLFPSNDDTDKHRHGWINFVLDPLNDYFIGLM"
     gene            complement(9839..11320)
                     /locus_tag="PKX59_04595"
     CDS             complement(9839..11320)
                     /locus_tag="PKX59_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HOZ90179.1"
                     /translation="MKIKNITTLGDLKKNGYKSRSIKEEMRENLIRKLKSGEKLFEEI
                     QGYEDTVIPQLQTAVLSKHNIILLGLRGQAKTKIARLMTQLLDEYMPIVSGSELNDDP
                     LNPLSRYAINIIEDEGDTTPITWIHRNDRYTEKLATPDVSVADLIGDIDPIKAAAQKL
                     NYSDERVIHYGLVARSNRNIFVINELPDLQPRIQVALFNILQEGDIQIRGFKLRLPLD
                     IQFVFTANPEDYTNRGSIITPLKDRIGSQILTHYPKNIEIAGAITLQESKIEKSQSEN
                     IFIAPLLHQLIEQTAMEARQSELVDQKSGVSARLTISALENLYSTCEQRMLLNHENKS
                     NGRISDLQGIIPSITGRVELVYEGEAEGIQSVALTLMDKAIRKVFAETFTDPEKLKKS
                     KNTNPYSEIINWFEQGNTLSLLHLDTTAQYIKKLDEVHGLKKTVKQFLKNINENEVHL
                     WMEFLLYGLAAFSQISKTALERNTEFKDLMRTVFNFKSMDDEV"
     gene            complement(11324..12418)
                     /locus_tag="PKX59_04600"
     CDS             complement(11324..12418)
                     /locus_tag="PKX59_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOZ90180.1"
                     /translation="MHGTRFTAFIPEKENTPFDKLLSIFMELLPYTAGDVSEALSWMT
                     ELDKQYKLTDKSYGMGDFIKDLKEKGYIKDNPDNDKFSLSSKSERTIRKRALEEIFGK
                     LKKSNTGNHKTSFTGQGDEWTSDLRPFQFGDNIDSINFTQSLQNAQINHGPDEFNLTE
                     NDLEIQEKEFKTQHSTVLMIDISHSMILYGEDRITPARKVAMALAELISIKYPKDTLD
                     IIAFGDDAFPIAIKDLPYLQVGPYHTNTTAGLQLAMELLRRKKTQNKQIFMITDGKPT
                     CIKSGASYYKNSFGLDQKIINSVFSLASQCRKQKIPITTFMIATDPYLQQFVRQMTAI
                     NKGRAFYSSLNGLGEFVFEDYVKNRKKRVL"
     gene            complement(12613..12984)
                     /locus_tag="PKX59_04605"
     CDS             complement(12613..12984)
                     /locus_tag="PKX59_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HOZ90181.1"
                     /translation="MATKKAKKAPAKKAAPKKKAAPKKKAAAPKKKAAAKAKTKRTPN
                     AAFMKAMTIGEALHPVVGSKPLPRTEVVKKLWEYIKKNKLQDPKEKRNINADDNLKKV
                     FGGKKTVSMFEMTKLVSKHLS"
     gene            13425..14855
                     /locus_tag="PKX59_04610"
     CDS             13425..14855
                     /locus_tag="PKX59_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90182.1"
                     /translation="MKKAIFITLTCVVALSTVRGQDMWGIANSNYAGNFAIDINPSSM
                     AVSPIKYELNILSGNMTFYNNYLYLPKGKMSVGKFFKADYFNYDWQDQYTGKDKNAFF
                     QTGIKGPSFFLRSGQTAFGLHTGLKAMGSVRNVDFEMAKLFYENSTYTPLQHTELEAG
                     NNKLAALLYGEIGASFAQQIEAGPGNRIAIGATFNYLMGFDGFYAQNDHMQYRFDDES
                     MLHVTDLQAEYGYAVPQDGKSDYLKVRGKGFSTTWGVQYIHGLNPEAYNVYASRLKFK
                     KYKYKIGMSLVDAGSILFAKDTRTVKFDNNNIDWSGFDTTGVESISQLGELIRSKVFD
                     DSTSGYVATKYRAKLPAAISIQFDYCLTHFLYLNTSIIQPVTSKSTAIRRPAQLSFTP
                     RLETWRYEISFPLTWYEYDQVRLGTCLRLGQVVIGSDYLTSMLGLSKFNGFDFFVGIK
                     HSSYAMDKLSKRKKEKVKDTYTGGDI"
     gene            14935..15933
                     /locus_tag="PKX59_04615"
     CDS             14935..15933
                     /locus_tag="PKX59_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HOZ90183.1"
                     /translation="MNIKQTEQFLKKVKGMHTLVIGDVMTDAYLWGRVDRISPEAPVP
                     VVAVQKRESRPGGAANVAVNLHSLGAIPFICSVVGKDNAGKEFREMITKSGMPADGIL
                     ASSKRTTTIKTRVIGNNYQLLRVDAEVAEPLSQADGEMLIKKAIEIIRKKKPAVIVFE
                     DYDKGVLNKTVIEAIVAEAQKQNIPTTVDPKKNNFFHYRNVTLFKPNLKELREGLKTD
                     LEKPTATALTKLASDFATKQKIKSLLITLSEHGVYYYSKNEKGIIPAHFRNISDVSGA
                     GDTVISVASLAIAAGFSCHEMAAISNLAGGLVCEKVGVVPIDGNQLLNEVKRLSGK"
     gene            15935..16654
                     /gene="ubiE"
                     /locus_tag="PKX59_04620"
     CDS             15935..16654
                     /gene="ubiE"
                     /locus_tag="PKX59_04620"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOZ90184.1"
                     /translation="MQVTPYNTGESKKEQVQHMFDAVAHRYDFLNHFLSLGIDKIWRK
                     KTIKEIGKLKPRTILDIATGTADLAIAATSLNPERIIGVDISEKMLAAGRVKIEAKNL
                     NNIISLQQGDSEQLQFENQTFDAVMVAFGVRNFQNLEKGLMEMKRVLNSNGKVFILEF
                     SKPTMFPIKQFYGIYSRYLLPLFGKLISGDASAYTYLPESVKHFPSGNDFALVLKQCG
                     FTNIEIKPMTFGIVTLYIAAA"
     gene            16651..17361
                     /locus_tag="PKX59_04625"
     CDS             16651..17361
                     /locus_tag="PKX59_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOZ90185.1"
                     /translation="MKQRVIVFSFLVLLFNADSLFAQKQKILNLPKYDRQRFHFGFIL
                     GINKTDFVIDRVASFSLIDSLYTVESKGTSGFNLQIVTNMRMGEHFDLRFLPGLAFAS
                     RNLIYTFEKGRPLSNVVTKKVESTFVELPLDVKFKSARLNNFRAYVLGGVKYSIDMVS
                     QAKVKAKDKEYVKLQRNDYGYEIGVGCDFYLPMFKFSPEIKMYHGLRNLLVKDDLVYS
                     KSLDGLFSKMFTLSLTFE"
     gene            17377..18933
                     /locus_tag="PKX59_04630"
     CDS             17377..18933
                     /locus_tag="PKX59_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOZ90186.1"
                     /translation="MQTFELVVLPEEAASESVIQHIVAKKFGGNRLFRIIKRSIDARS
                     KTVKIRLQLQWTDEQNQETLVENRKWRQVANSEPVIIAGCGPAGLFAAIRLIECGFKP
                     IIIERGKDVRNRRRDLAAINKQRQINPESNYCFGEGGAGTYSDGKLYTRSNKRGNIAS
                     VLNTFIEHGADGDIAVDAHPHIGTNKLPAIISAMRNRIVECGGEVLFDTKLVNIDFDT
                     SGVNGVEVQQGLAQFHIKSKALILATGHSARDVFYMLNNQKILIEAKPIAIGVRAEHQ
                     QQVIDSVQYHCSKRSQFLPPASYALKHQHDGRGIYSFCMCPGGIIAPAATDIGEVVVN
                     GWSPSRRNNVFANSGIVVSITEEDVLPFKNAKELRFLELQKSIEEKAFVSGGSNLAAP
                     AQRIVDFVNQRVSSNLPTCSYIPGINSVNLKEIFPASVTNSLAAAFVAFGKKMKGYYT
                     NEAVLVGTESRTSSPVRIPRDVETAMHVQVKGLFPCGEGAGYAGGIVSAAMDGEFCAE
                     KCAAYLKNNN"
     gene            18966..20051
                     /locus_tag="PKX59_04635"
     CDS             18966..20051
                     /locus_tag="PKX59_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOZ90187.1"
                     /translation="MEINQPPYYIRITFKILLAILIFYVLHAAQDVLIPLTVAVLFTF
                     LLMPVSNKLIKWKFPPAIAILCSILLAFVAVGGLLYFFYSQIVSFAEDLPLLQAKLLE
                     KVEFLQRFVNETFRITKEQQNVLVKSKLQENIADSGLLLLNLFSSTGTFIANFALVTV
                     YVFFLTLYKKKFKQFIEAIVSDGKHNSYFQIVKKVAGVSQNYLKGIFLDVLILSILNS
                     TGFLLLGIEHAVLFGVLASVLNIIPYIGVLIGSVLPVLMALLTKDEIGYAIGAAGVCV
                     TVQFIDNNFITPYVVGSSVSINPLTATIALIISASVWGLAGMVLCLPLTGMLKVVCDH
                     VEGLKPIGFLIGEEVDYRKNKFKLGRK"
     gene            20131..20784
                     /gene="scpB"
                     /locus_tag="PKX59_04640"
     CDS             20131..20784
                     /gene="scpB"
                     /locus_tag="PKX59_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOZ90188.1"
                     /translation="MENLKQHIEALIFTSEQSITANEIAAALKASFGWELSNENIRQS
                     ISELINKYSADDYSFELVEIAGGFRFLSKKEYYGVVNTMLQINSKKRLTTAALETLAI
                     VAYKQPVSKSEIENIRGVNCDYSIQKLLEKDLITIQGKGDGPGKPILYGISRTFMDHF
                     GLKSAKDLPKLKDVILDENEIGTPADMQYAENDNAEGVSITLGTQEGNTDKTDDVAE"
     gene            20815..21648
                     /locus_tag="PKX59_04645"
     CDS             20815..21648
                     /locus_tag="PKX59_04645"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOZ90189.1"
                     /translation="MKISVLGLQFDSALKNVFSMLSIALICLMQPSISEGQEKLRKVE
                     NQAFTYGEVLEYRVHYGIIDAGEAKLAVMPDKKTIGPREVYHVVGTGQTKGAFDWFFK
                     VRDRYESFIDAQAIIPWLFVRRVYEGGYKINQDVTFNQVKNLAISKKATIATPQNVQD
                     LISAFYYARTIDFSNAQPGALYDVDAYLDDAIIPVKFKYVGKENISTKFGTFRCLKFH
                     PQLLEGRVFKNKEDMTLWVSDDENKIPIRAEAKILVGSVKMDIKSFSGLANETLSLLK
                     K"
     gene            complement(22019..22837)
                     /locus_tag="PKX59_04650"
     CDS             complement(22019..22837)
                     /locus_tag="PKX59_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90190.1"
                     /translation="MFAVATAFNGSAQFLKTDSVGNALWNVGFTDSTFYNLFFDQITP
                     DSGNTFVTAGNFDIFNPSINAAARIDSAGNLLSMHQYNNYSNCHSYSHYFNPQTGLAM
                     FGYGGGFNNFGFYFCKTDSVLTTPCMSTPSTFTTYSYSVTDSATSILSQPVSLVSIDV
                     LAQMSVNSISFTDSSLCPLVSSVPSFQNQNTLQVLNADVSLYPNPFSSQLTFSLASNE
                     KTTASLYNFVGQRVLQQTFSNSTTINTEQLPEGIYYYALHNYKGVFKTGKVIKQ"
     gene            complement(22861..23592)
                     /locus_tag="PKX59_04655"
     CDS             complement(22861..23592)
                     /locus_tag="PKX59_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90191.1"
                     /translation="MKKLLQFSFILATYLSSGQTAFEKMFKPSGVSDIFLTYYDVIKS
                     PDDSGYYTLSTFGGPDSGAILSLVGNDGILKWSKSYQRVTAIFKTFGLSVLPLSDSTL
                     MLNLYDDGRTIQLKTSYSGTMLGAKKLNYISSTVYFQEEKNNAIYGVGRVSANGVNGG
                     LFCKMDLDGNIIWSRMMNGGTSRRIELRALTLTSDGGFLLAGTRQIYGTILPASTILI
                     KTDSIGNITWQRQLNYVNKILFLLP"
     gene            complement(24145..26184)
                     /locus_tag="PKX59_04660"
     CDS             complement(24145..26184)
                     /locus_tag="PKX59_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90192.1"
                     /translation="MKNLIAIIFCLLIFETVNAQWTQTNGPEGGDISSIVTDGTNLYA
                     GTYYGGVYRSSNNGQMWTAINNGLTHIRVGTLWLNGADLFAVTDGGLYVTSNNGNSWS
                     LINIVLPPFTSVTTFAANATNLFIGTSDNGVYTSTNNGVSWSVANSGLPLIQINSIAI
                     SGNNIFAVTQFQGVYLSTNNGVSWVAVNTGLTDLHISHLAIDAGNIFAGSIYGGVFLS
                     TDTGNTWNALNNGLTNLHVSAIAASGANVFAGTAGGGVFFSSDTGNTWTSANNGLTSS
                     TIYSFLINNSDIFVGTSADGLFLSTNNGASWNAQNTGLASSFVHSLAVSGSNLFAGSN
                     YKGMYRSTNNGNSWSQINNGLTSTFINSILATTNDLFVGTVNGLFMSADTGNTWVQSD
                     SGITNKFIIAVAKLGSHLIAASYLNEVYLSVNNGSTWNLINNGLPTGGSSSLVISGSN
                     IFLGTYQNGVFLSTDTGNTWNAVNNGLTNLHVSSLAISGTNIFAGTDSNGVFFSSNNG
                     NSWNAVNNGLPTTSIITCLAINGTNILAATMQDGVYFSNNNGTTWSAVNNGLTNKIVT
                     DLTISGINFFAATQGNGVFLNSNLLTDITENTNYYSSINIYPNPALSQVVIEFNHVID
                     NGKIQMFNTFGNKIIEENIISESKKVINLNNISNGIYFMKVFDGKNSNCKKLIVE"
     gene            complement(26447..>27429)
                     /gene="pdxA"
                     /locus_tag="PKX59_04665"
     CDS             complement(26447..>27429)
                     /gene="pdxA"
                     /locus_tag="PKX59_04665"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOZ90193.1"
                     /translation="TFADSRMLSVCTPVIYGSSKVISYHRKSLHQQEFNFNTIRTAEE
                     ASHKKTNVINCWEEDVKVELGKPSTIAGTYAFKSLEAAGKDVLDKKIDAIVTAPIDKS
                     TIHSDAFPFKGHTEYFAKLCGNAEHVMLMVSDKLRVGLITGHVALKDVPAQLTKEKII
                     AKTKLVHQSLRSDFGLQKPRIAILALNPHAGDNGLMGKEEQEIITPAISEMYNAGIGA
                     FGPYPADGFFGSSRFKSFDAIMAIYHDQGLVPFKALAFSTGVNYTAGLPIIRTSPDHG
                     TAYDLAGKNQASEDSFREAVYLACDIAKQRQITSEITANPLKFSRMSADR"
BASE COUNT         8845 a         4516 c         5357 g         8624 t
ORIGIN      
        1 aaaaacacac aaaacacaca ccaacaacaa caccaaaacc aacaaaaaaa acaataattt
       61 aaataaaaaa cattaatata acatgccaag cggtaagaaa agaaaacgcc ataaaatggc
      121 tactcacaag cgcaagaaga gattgagaaa aaacagacac aagaaaaaat aatctagtgt
      181 ttgcggattt tgcaggggta tattatctca gtacaaatct gttttttggg gttattttac
      241 cctctaatca attaaaattt gcgtcttgag caacgattta gttatagact caagtccatc
      301 ggaagtacgt attgccctcc ttgaagaaaa aaagctggtt gaattaaaca gcgaaaagaa
      361 ggacagaagt tttgccgttg gcgatattta cctgggcaag gtaaaaaagc tcatgccttc
      421 actcaacgct gcatttgttg atattggcta tgagaaagat gcttttttgc attatctgga
      481 tttaggcccg cagtttcagt cgctattaaa atttgtaaaa gacacacaaa gtggaagaca
      541 gacaaatgcc acactgaata attttgaact tgaaaaggat attattaaga ccggcaaaat
      601 aactcaggtg atgagttccg gtttgtggct gcctgttcag attgcaaaag aacctatatc
      661 aactaaagga ccaagaatta ccacagaaat atccattgca ggacgctact ttgtggtttc
      721 accttactac aatcatgttt cagtttcatc aaaaataaag agtccggaag agcgtaatcg
      781 tttgaaacgt cttgccctta gtattaagcc aaaaaatttt ggtatcattg tcaggacagt
      841 tgcagaagga aaaaaggttg cagaacttga taaggatatt caggacgtgt tgatgcgctg
      901 gaatttattt tatgacacat taaaagtagc acagccggga caaaaggtgt tgggagagat
      961 agatcgcact tcatctatcc tgcgcgatat gcttaatcct aattttaacc atattcatgt
     1021 taatgacaac cttctttatg acgagattaa gtcttaccta agaagcatag cacctgagca
     1081 agaagagata gtaaagttgt ttaagaataa gcaaccgata tttgaacatt tcgggattga
     1141 caagcagata aaatctgact ttggaaaaac cgtagccatg caaaacagtg cttacttggt
     1201 gattgagcat acagaagcta tgcatgttat tgatgttaac agtggaggac gaaaccgggg
     1261 cgatagcgat agtcaggaaa aaaatgcttt tgaaaccaac tgtatggcag ctaaagaaat
     1321 tgccagacaa ctcaggttgc gcgatatggg cggtattatt gtagttgact ttattgacat
     1381 gattaatctt gaacacaaac gtcagttaca tgaagtgctc aaggaagaga tgaaaaaaga
     1441 tcctgctcgt catactgttt taccaccaag taaattcgga ttaattcagt tgacccgaga
     1501 gcgtgtgcga ccggaaatga aaatttcaac aactgaaaaa tgtccgtcat gtaatggcac
     1561 cggtgaaata aaagccagca ttttacttat tgacgaaata gagaacaacg ttcgctattt
     1621 tgcacatgaa caaaacgaaa agaaaattat tttacatgta catccatata ttgaagcgta
     1681 tttgaataaa ggattgattt cgataaaatg gaaatggtat ttacggcata aaataaaagt
     1741 gagtattgag gcagtaagtt cgtttgctgt aacacagttt gctttttata ataccaaagg
     1801 cgaagagata aagatataga gagattagaa tgtttcacat tcataatatc ttgcaaatta
     1861 tattcaagga tagattttaa atacactacc ttagcattcc ttaaaaacca ttcaaaataa
     1921 tgagaaaata tttcattctg ttagttgttg ttttctgtat ggacagtact aatttatttg
     1981 caggaaaaga atcatctgtt acagacaaac gtttcgattc ttttaaagaa agatttataa
     2041 aagacctgtg gaaacataat ccaatgtggg caactgaaat aggttttcat gcatacgatt
     2101 ctattcttga aattaataat gaacaatatc gtaatgggca ggtagctttt tcaaaatcat
     2161 atctcgattc tttaaagaca tttgataaag ttgctctaag tagtttaaan nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnngctg gcagtggaat ccatcatcat acaatgcaac aggcaatgta
     2341 gcatttatgt tatctgaaaa ctatgagccg cttaataagc gattagtaaa tattggcaaa
     2401 aaactctcgc agtttaaatc ttattacgag gtagcaaaaa agaacatagc aaacccaact
     2461 gatgaacatc ttaagttggc aatggagcaa aatgcaggtg gcattgccgc acttgagaca
     2521 gatttattag actcactgaa acaatctaca ctaaaggccg atgttaagct aaacattact
     2581 caatcactga ctgatgcaaa aaaagctgtt gaagattata ttgcattttt aaaagacttt
     2641 aagaatgaaa acccaagaag ttttaggttg ggaaaagatt tgtatgcaaa aaagtttgaa
     2701 tacgaaatac agagtcaata tacgtatcag cagatttatg attctgcaat gcaacgaaaa
     2761 tcttatttgc ataatgagat gtttaaaatc tcagacaagt tatggtcaaa atattttgga
     2821 acaaaggcta aacctgtaga tagtttagca atgattaaaa tgatgataga taccatttct
     2881 gtacgtcatg taaagcctga tgaatttcaa tcagcaatag aaaaacaact tcctgaattg
     2941 gttaaattta tcaatgaaaa aaatctgatt tatattgatc cttcaaaacc attaaaagta
     3001 aggagagagc ctgcttatat ggcaggagta gcaggtgcat ctatcagttc acctgggcct
     3061 tatgataaag gaggtaatac atactataat gtgggttcac tctcaggttg gtctgctgaa
     3121 cgtgcagaaa gttatttacg agagtataat cattacattt tgcagatttt gaatattcac
     3181 gaagcaatac cgggacacta tacgcaattg gtctatagca ataatgcacc aagtattatt
     3241 aaggcagtat ttggaaatgg cgccatgatt gaaggatggg cagtttatgg ggagttgatg
     3301 atgcttgaaa atggctatgg tgacaatgaa cctgaaatgt ggctgatgta ttacaaatgg
     3361 aatctgagaa cagtttgtaa taccatttta gatatttctg ttcacactaa ggatatgaca
     3421 gaggcacagg cattaaattt attggagaat gaagcatttc agcaaaaggc agaagcacaa
     3481 ggtaagtgga ggagagtaag tgtaactaat gttcagctta caagttatta taccggtttc
     3541 aaggaaattt atgacttacg cgaagagtta aaaaagaaaa tgggaagcaa gtttaatctg
     3601 aaagcttttc atgagaaatt tttgagttac ggtagtgcac ctgttaaaat gattcgtgag
     3661 gaaatgctct ctgagataaa ataacatgat tcgtttcgag cgctttttaa ctttaattaa
     3721 acgacagttt tatatttatg taggcggact ctttctgctt ctgtcaattc gtttattttt
     3781 tctttttcga ttattcagca ttgctgaaat taagttaaat gttgttgata tattacatgc
     3841 attggctgta ggtttcagat ttgacacaat ggtgctgatg tatggaatgg cattacctgt
     3901 tttgttaagt attttatctc tgattgttgg aactgcaaag agcaaagata tggtgatgcg
     3961 ttttaacaga gtttatgcaa tagtcatgtt gatgcttttt gtaattgttg gaatcgtgga
     4021 tgtttattat tacaattatt ttcagtcaca tatcaatgtg ttagcttttg gtttgttcga
     4081 tgacgatact gctgccgtgt taaaatctgt ttggacagac tacccaatta tgagtgtaat
     4141 agctgcatgg ataattgttt tttttctgat cagattattt gtgaactatg tttttagtga
     4201 taaagtaaag cctgttgtaa ttaataaagt ttggcaaggt gctgtatgta gtttgttagc
     4261 gttagcaatg ttttttcttg gaatgcgtgg ttcgataggc acatttccat tgcagattga
     4321 tgactctaca gtttgtaata acagcactat aaatctgatt ccgataaatg gtgtgtttgc
     4381 aataaaggaa gcatttctga tgcgtagcaa gcagatgagt attgaaggca acataacctt
     4441 cttgaaaaaa ttgggttatg gtagtgaagt aaaagctatt gaagattatt atggtcatgc
     4501 accggaaaaa gatttaaaca aatcatactt tacagtaaca cctaataagc cttctggatt
     4561 taagcatcct aatgttgttt tttttctgat ggaaagcatg agtaataata atttgtttta
     4621 tcattcagct acacttaatc tatatggtgc attggaaaaa catctttcaa cagattattt
     4681 tttaagaaat tttttaccat caggtaatgg tactatcaac agccttgaag gcataatggt
     4741 caatacaccg gtgacatcat tggcacagtc agaacataat tcggtttctt attccagttc
     4801 tgttgcattg ccatttagaa attcaggtta tgaaacaaca tttatcacag gcggtaagtt
     4861 tggctggcga aacatcaatg agtttattcc tcatcagtat tttcagcatg tggaaagtaa
     4921 agataatgtg ctgaaagaaa ttgcaggctc aacagagtgc gaatggggcg tttatgatga
     4981 gtacttattt gactatgtgt tcaaaaagtt ggaaaaggcc actaagccac aaatgatttt
     5041 tgtactcacc acaacaaatc atacaccttt tcatttgccg gaaacatata gaccatatcc
     5101 tataaaactg actaatgaaa taaactcaaa attacttacc gatgagaacc ttgctgttaa
     5161 gaatcttact aatctgcaat attccaatga ttgcttaggt aaatttatgg ataaggttaa
     5221 gcactccaat ctcagtcaaa atacaatcgt tgcagctaca ggcgaccata ataatttaat
     5281 gctatttgat tttaatgaaa gtcagatgta ctaccagcga agtgttccac tgtatatgta
     5341 tgtgccggaa acaattcatc cacatgtggc aatcaacaaa tggggttcac acaaggatat
     5401 ttttccgact ttaataaatc ttgcactgcc acaatcaact tattttaatg ccggagataa
     5461 tttgtttgac acattgcaaa gtcgcaataa tatttttgca acagactgga tgagcatgga
     5521 agccatgaat gaatttggtg ctgtcaagta tggtgagtct gtaaaatatt ttgattggga
     5581 taagaataaa atgctgaaag aaacagcaac accatctgcc gatttgcaaa aattacttca
     5641 acgcgcaaga gcacactatg ctgcaatggc ttattttatc agaagtgaaa ctttaaaaac
     5701 taaacaagga aagtagtgtt taatttgttg gtttcaatag aaaaagtttg ataatagaaa
     5761 taagtgaaac caatgcccat gcagcttcaa gaataataaa gggcatataa tgaatcagaa
     5821 cagaagccaa gcatgcaaga gaagcgccaa taaaattcag aataagataa aagagcttat
     5881 ccggtttaat aattcggaaa agatgaagga aatatgccac taaaaggata aaaacaccaa
     5941 taaatccaat aaaatcttcc ataaaatgtt taatttaacc agaaccaaag gtatggcaat
     6001 ggttgtataa tttatcttcg tacgtttaaa ttttgtaaca atgaaattcg caactaaagc
     6061 acttcatgca ggcgttgagc cagatccaac taccggtgct atcatgacac ctatttacca
     6121 gacttcaacc tatgtacagg catcacccgg acaacataaa ggttatgcct atgctagagg
     6181 aaaaaaccct acccgttcgc agttagagaa aaatattgct gcattagaaa atgcaaaata
     6241 cggattgtgt ttcagcagcg gaatgggagc taccgatgca gtgatgaagt tgttaaagcc
     6301 cggtgatgaa gtaatttccg gtgatgattt gtatggtgga acatatcgta tgtttaccaa
     6361 ggtttttgca aactatggta ttaagtttca ttttataaat ctaactgatg ctaacaatat
     6421 cagtaagtat atcaattcaa acacaaaact catttgggtt gaaacaccaa ccaatccaac
     6481 catgcagatt gttgacataa aagcttgcag cgcaattgca aaaaagaaca attgtatttt
     6541 ggctgttgac aacacatttg catcacccta tttgcagaat ccaatggatt taggtgcaga
     6601 cattgtaatg cattcagtta ctaaatatct gggcggacac agtgatgttg ttatgggtgc
     6661 attgtgtatg agtgatgatt cattatatga aagacttgcc tttattcata actcatgtgg
     6721 tgccactccg ggacctatgg acagttttct tgtattaaga ggaataaaaa ctttacatct
     6781 tcgcatggag cgtcattgtt ttaatggaaa acaaatagca gcatttcttc gtgctcatcc
     6841 aaaaatcgaa aaagtttatt ggccgggctt tgaagatcat ccaaaccatc acattgcaaa
     6901 aatgcagatg cgcgattttg gtggtatgat ttcttttgac ttaaaaagtc actctcagga
     6961 agaggctttt aagttagcgt catcggtaaa agtgttttca ttggctgaat cattgggtgg
     7021 agtagagtct ttggtaaacc atcctgcatc aatgacacat gccagtgttc cggttgcgga
     7081 aagagtaaga gttggtgtaa ccgactcatt attgagatta agtgtgggtg ttgaagatat
     7141 agacgattta attgcagatt tgaaacaagc aatagggtag ttccttcaca aaaaatttac
     7201 attgaacata tgctcaaaac acacgatttt attacttttg attcattata aataaattaa
     7261 aacaatgcta aaaaaatcta ctaatttttt attactgatg atggccatga ttgcaaccat
     7321 tacagtcaat gcacagaaca cacattggtg tgctactgat gaagttacta atgctgcact
     7381 gcagaatagt gctgctgccc gtgctgcaca aaatcagtta gaagcgttta cgcaacagta
     7441 cgtacaaaac tttcaaccaa gctatttcag aagtactggc actgctgtgt atgttatccc
     7501 tgtggtattt catgtcattc ataattgggg tcctgagaat atttcagacg cgcaggttta
     7561 tgatgcaatc agagttttga accgcgatta taacggacag cgaacagata cagcaaacat
     7621 catccctgat tttaaagcat tgaatgccga tgttcagatt gaattccgtc ttgcacataa
     7681 agatcctaat ggaaattgta ctaatggtat tgacagacaa caatcgttgt taacttatga
     7741 tgccggtgat caatcaaagt tgaatccatg gccttacaat aaatatttaa atatctggac
     7801 tatttctaac ttcggttcat cgcatgcaca agcggcagct tatgcataca aacccggtac
     7861 agcgcctccc ggtaaagatg gaatcattgc attgcataac tacgttggta gcatagggac
     7921 tggtagttca acaggtcaat atacacttac gcatgaaatt ggtcattggt taaatctgaa
     7981 tcatacatgg ggtggaacca atcaacccgg tgtagcttgc ggtgatgata acgtaacaga
     8041 tactccggta actaaaggtt atactacatg tccatccgct tcaaatgcag atgtgtgtaa
     8101 cccgggtgtt atagaaaact atcaaaacta catggactat agctattgct attacatgtt
     8161 tactttagga cagaaagcgc gtatgtgggc tgcattaaat tcaggtgcag gcttgcgtaa
     8221 tcaggttcat actgcaacca atttaactaa tacgggaacc gatggaggta cgtatgtttg
     8281 tgctcctaca gcaagttttt cacccgtaca aaaagtaatt tgcgaaggcg gaactgttag
     8341 ctttagtgat gcaagtttaa atacagatac agtgggcaca acttatctgt ggacttttaa
     8401 cggtggtact ccatcaacct caacagataa aaatccaaca ggtatcattt ataatactgc
     8461 tggtatttac gatgtaacat taactgttac tacttctgca ggttttgata cttatacagg
     8521 tattgaaagc gttgttgtaa gaccgcagca ggtttctaac tttgttccat attcagaggg
     8581 ttttgaaaat gtgttattcc cttctgtaga ttggattgtt gataatgaaa atggcaatgc
     8641 atgggaactg aattctaccg ctgcacattc aggatttaat tctgttagaa taaataattt
     8701 cagtggcaat gttgcgggta ccaaggatgt atttattaca ccaggtttta atttaacaaa
     8761 cactacacaa acacaattta ctttttggct agcttatgca ctaaagaaca gctccggtac
     8821 agatcagtta aaagtttatg cttctaacaa ctgcggtcag ttgtggaacc tgcgtacaac
     8881 aaaaacagga acgcaattgg caacagtaag ttcaacagta acttcagatt ttgttcctaa
     8941 tgcagcagac tggcgtcagg aaacagtaag tcttacttca ggacagtatt caactaaacc
     9001 aaacgtttct ttaaagtttg aatatactca cgataagggt aataatattt atattgatga
     9061 cattaacctt acaggtgttg ttggtatcag cgactatgct gtttcggcaa gtgatgtaac
     9121 tattgttcct aatcctgctt cagagaagtc aagtgtaaac tttgaattgc tgcaacctga
     9181 aaaagtactg attacagtaa ctgatatgtt gggtaaagaa gttcatcgaa tttcagaaaa
     9241 ccgtttagat gctggagaat atcaatatga agtaaacaca aattgggcta agggagtata
     9301 tacagttcgt attgctgccg gaagtcagat tatcagtaaa aaattagtag tagaataaat
     9361 taagtaaata ttaattaagc ctgaagctga atttggtttc aggcttttct ttttgatggt
     9421 tatgagtgaa agtaaatttt caattgaaat gaattatgtg gtttatgcaa aaccggaagc
     9481 ggtgtttaat gcattgacaa atcctgatgt gatgaaaaaa tggatagaag gaagttttgt
     9541 ttttgaattg aaaaaaggag gtaatgttga attgtttgac ggttgggtta agggcgaagt
     9601 tattacgttt attaacaatg aatcattcag ctatacctgg aaacccaata catgggataa
     9661 aaagaccaaa ccaagcttag tggaaattga gttgaagccc gatgctgccg gtacaagagt
     9721 gtatattcat cacagtttgt ttccaagtaa tgacgataca gataaacaca gacatggatg
     9781 gatcaatttt gtacttgacc cgttgaatga ttatttcatc ggattgatgt aataaagttt
     9841 aaacttcatc atccatagat ttaaaattga atactgttcg cattaaatct ttaaactctg
     9901 tgtttctttc caaagcagtt ttgctgatct gagaaaaggc agcaagtcca tacaatagaa
     9961 attccatcca tagatgaact tcgttttcat ttatgttttt cagaaattgt ttcactgttt
    10021 ttttaagtcc atgaacttca tcgagctttt taatgtattg tgctgttgta tcgagatgta
    10081 aaagactcaa tgtatttcct tgttcaaacc aatttataat ttctgaatat ggattggtat
    10141 tttttgattt ttttaatttt tcgggatcag taaatgtttc tgcaaaaact tttctgatag
    10201 ccttatccat caaagttaat gctactgatt gaatgccttc ggcttcacct tcatagacca
    10261 attccacacg cccggtgata gatggtatta tgccttgcaa atcgcttatg cgtccattgc
    10321 ttttgttctc atgatttaat aacatccttt gttcgcatgt gctgtacaaa ttttcaagtg
    10381 cagaaatggt cagtcgtgca ctcacaccac ttttctgatc aaccaactcg ctttggcggg
    10441 cttccatggc agtttgctca atgagttgat gtagtaaagg agcaataaaa atattttcag
    10501 attgagattt ttcaattttt gattcctgca aggtaatagc tcctgctatt tcaatattct
    10561 ttggatagtg tgtaagtatc tggctgccta tacggtcttt taagggtgtg atgattgaac
    10621 ctctgttggt ataatcttcg ggattggcag taaacacaaa ttgaatgtct aaaggcagac
    10681 gcagtttgaa accgcgaatc tgtatatcgc cttcctgcag aatgttgaac aatgcaactt
    10741 gtattcgggg ttgtaaatcc ggaagttcat tgataacaaa aatgtttctg ttgcttcggg
    10801 caactaaacc ataatgaata acgcgctcgt cagaatagtt taacttttgt gcagcagctt
    10861 taataggatc aatgtcacca atcaggtcag ctacagaaac atctggagta gctaattttt
    10921 cagtgtaacg gtcgtttcta tgtatccatg ttatgggtgt ggtgtcgccc tcatcttcaa
    10981 taatattaat agcatatcgc gaaagcggat tcagaggatc gtcatttaat tcagaaccac
    11041 ttactatagg catgtattca tccaacagtt gtgtcatcag tcgtgcaatt tttgtctttg
    11101 cctgaccgcg tagtcctaaa agaatgatgt tatgtttaga taagacagca gtttgcagtt
    11161 gtggaattac tgtatcttca tagccttgta tttcttcaaa taatttttcc cctgatttga
    11221 gtttacgaat caggttttct cgcatttctt ctttaataga cctggacttg tatccgtttt
    11281 ttttcaaatc gccaagtgtt gttatatttt taatcttcat tggttataga actctttttt
    11341 tacggttttt tacataatct tcaaatacaa attcgcccaa tccattcagg ctgctgtaga
    11401 atgcacggcc tttatttatt gcagtcattt gtcttacaaa ttgctgcaaa taagggtcgg
    11461 tagctatcat aaaagtagtg ataggaattt tttgtttgcg gcattgtgat gccaacgaga
    11521 aaacactgtt aattatcttt tggtcaaggc caaaactatt tttatagtaa gatgcacccg
    11581 attttataca agtcggtttg ccgtctgtaa tcataaaaat ctgtttgttc tgtgtttttt
    11641 ttctgcgtaa taattccatg gcaagttgta gtcctgctgt agtattggta tgataaggac
    11701 caacctgcaa ataaggcaaa tcttttatgg caataggaaa agcatcatca ccaaatgcaa
    11761 taatgtctaa agtatcttta ggatatttta tactgataag ttctgccaat gccatagcta
    11821 cctttcgtgc gggtgttatc ctatcttctc cataaagtat catggagtgc gatatgtcaa
    11881 tcatcaaaac tgttgaatgc tgggttttaa attctttttc ctgaatttca agatcatttt
    11941 ctgttaagtt gaattcatct ggtccatgat taatttgtgc attctgcagt gattgagtaa
    12001 agttgatgct gtcaatatta tcgccaaatt gaaagggacg taaatcagaa gtccattcat
    12061 ctccctgacc ggtaaacgat gtcttatgat ttcctgtatt tgatttttta agctttccga
    12121 aaatttcttc caatgcacgc ttacgaatag tcctttcaga ttttgaactc agactgaatt
    12181 tatcgttgtc gggattatcc ttgatataac ctttttcttt cagatcttta ataaaatcac
    12241 ccataccata acttttgtca gttagtttat attgtttatc aagttctgtc atccatgata
    12301 aggcttctga aacatcaccg gcagtatagg gtaacaactc catgaaaata ctgagtagct
    12361 tatcaaatgg agtgttttct ttttcaggaa taaaggctgt aaaacgtgtg ccgtgcatga
    12421 tgtgtgttat gggagtttaa aaatacaaca aaccaattaa caactggata ctcggaatgt
    12481 ttacggtata aatttatgag aattggacaa gacattgcca ttgtgtgatt agtttttcat
    12541 ggtacgacat ggctaaatga aaaaagggga ggctttttaa agcctcccct ttttgttctt
    12601 tcttacagaa tattaagaaa ggtgtttgct aactagttta gtcatttcaa acatagaaac
    12661 tgtttttttg ccaccaaaaa cttttttcaa gttgtcatca gcattgatgt tacgtttctc
    12721 ttttggatcc tgtaatttgt ttttcttaat atattcccaa agttttttaa caacttcagt
    12781 acgtggaagt ggcttgcttc ctacaactgg atgaagagct tcgccaattg tcatagcttt
    12841 cataaatgca gcatttggag tacgctttgt tttagctttt gctgctgctt tctttttagg
    12901 agctgctgct tttttctttg gagcagcttt cttttttggg gctgcttttt tagcaggtgc
    12961 ctttttagct tttttagttg ccattttgaa atttgttttt tttaatttgt tgagcaaatg
    13021 aaatacataa tttttgcttt gaagcaaccc tgtacattaa tttattaaca gctgttttca
    13081 gagggtaaac tgacaaattc ataacatcag tcactctgtg gtgttagaat aatattttag
    13141 tataatacat gttttctcct ctgaaaaata tgtttttacc aatgaaaaat caatgtaatt
    13201 tattgttttt acctctgaaa ataccatgtt gacagaagct gttatattgt taataaccga
    13261 agtcattaaa gaagtaataa agtttttaca agggaaaagt tgttgatttt ctctgaatct
    13321 tacagccaaa cttacatcaa taatagcagt aaggttatca ctttatagac tcaaccaaca
    13381 cttggatttt agcgtttagc agcacataac aattgtttta actaatgaaa aaagcaatat
    13441 ttattacact cacatgtgtt gttgccctgt caacggtcag aggtcaggat atgtggggaa
    13501 tagccaatag taactatgca gggaattttg ccatagatat taatccatct tcaatggctg
    13561 tttctcctat aaagtatgag ctgaatatac tttcggggaa tatgactttt tataacaatt
    13621 atctttattt accgaaaggt aaaatgtctg tagggaaatt ttttaaggca gactatttta
    13681 actacgattg gcaagatcaa tatacaggca aggataagaa tgcatttttt cagacaggta
    13741 ttaaaggtcc ttcgtttttt ctgcgctcag gacagacagc ttttggatta catacaggat
    13801 taaaggcaat gggaagtgta aggaatgttg actttgaaat ggcaaagttg ttttatgaga
    13861 attctaccta cacacctttg cagcatacag aacttgaagc cggcaataat aagttggctg
    13921 ctttattata tggtgaaata ggtgccagtt ttgcacagca gattgaagca gggcccggaa
    13981 acagaatagc aataggtgct acctttaatt acctcatggg ctttgatgga ttttatgcac
    14041 agaacgatca catgcaatat cgctttgatg atgaatctat gttgcacgta accgaccttc
    14101 aggctgaata tggttatgcc gttccgcaag acggcaaaag cgattaccta aaagttcgtg
    14161 gtaagggatt tagtactaca tggggtgttc aatatataca tggactgaac cctgaggctt
    14221 ataacgtata tgctagccgc ctgaaattta agaagtataa atataagata ggtatgtcat
    14281 tagttgatgc cggaagtata ttatttgcaa aagatacccg tacagtaaag ttcgacaata
    14341 ataatataga ctggagcgga tttgatacaa caggagttga aagcatcagt caacttggag
    14401 aacttattcg ctcaaaagtt tttgatgact ccacatcagg atatgtggcc acaaagtacc
    14461 gtgctaaact acctgcagct atcagtattc agtttgatta ctgcctgact cattttcttt
    14521 atctgaacac ctcaataatt caacctgtca ccagtaagtc aacagctatc agacgaccgg
    14581 cacaattgtc ttttacacct cgattagaaa catggcgtta tgaaatttct tttcctctaa
    14641 catggtatga gtatgatcag gtcagacttg gaacatgtct tcgtttaggt caggtagtta
    14701 taggttccga ttatcttact tccatgctgg gtttgtctaa atttaatggg ttcgactttt
    14761 ttgttggaat aaaacacagc agttatgcca tggataagtt gagtaagcgt aaaaaagaaa
    14821 aagttaaaga cacttatacc ggaggcgaca tttaaaaact acccattgct gccattttaa
    14881 tcgtttaaga aaagtagttc acattactta cttttgccct tcaattcaat aggcgtgaac
    14941 attaaacaga cagaacagtt tctgaaaaag gttaagggaa tgcatacctt agtaataggt
    15001 gatgtaatga ctgatgctta tctgtggggt cgtgttgacc gtatatcgcc agaagcacct
    15061 gtgcctgttg tggctgttca aaagcgcgaa agccggcccg gaggagctgc caacgttgca
    15121 gtaaaccttc attctttggg tgcaatacct tttatatgtt ctgttgttgg gaaggataat
    15181 gcaggaaaag aattccgcga aatgattacc aaaagtggta tgcctgcaga tggaatatta
    15241 gcatcttcaa aaaggactac aaccataaag accagagtta taggcaacaa ctatcagcta
    15301 ttgcgtgtag atgcagaagt agccgagcca ctgtcgcaag cagacggtga aatgctcata
    15361 aaaaaagcaa tagagataat cagaaaaaaa aagccggccg ttatagtttt tgaagattat
    15421 gataaaggtg ttcttaataa aactgtaata gaagccattg ttgctgaagc acagaagcaa
    15481 aatattccca caacggttga ccccaaaaaa aataattttt tccattatcg taatgtaact
    15541 cttttcaagc ctaatctaaa agagctgcgc gaaggactaa agactgattt ggagaaacca
    15601 acggcaacag cgcttacgaa gttggcatct gattttgcaa ctaagcaaaa aattaagtca
    15661 ttgctgatta cgttatcaga acatggagta tattattatt caaagaatga aaaaggaatt
    15721 atccctgcac acttccgaaa tatcagtgat gttagtggtg caggcgatac agtaatcagt
    15781 gttgcatctt tagcaattgc agcagggttt tcatgtcatg aaatggcggc aataagcaat
    15841 ctggcaggtg gcttggtttg cgaaaaagtt ggcgttgttc ctattgatgg aaatcagttg
    15901 ttgaacgagg ttaaaagact ttccggaaaa taaaatgcaa gttacgccct ataatacagg
    15961 agaatcaaag aaagagcagg tgcaacacat gtttgatgcc gtagcacacc gttatgattt
    16021 cttaaatcac tttctgtcat taggcattga taaaatctgg agaaaaaaaa ctattaaaga
    16081 gattggtaaa ctaaaaccaa ggactattct tgacattgcc accggtacag ctgatttagc
    16141 aatagcagca acatctctta atccagaaag aataatagga gtggacattt cagaaaaaat
    16201 gcttgcagca ggaagagtaa aaatagaagc aaagaacttg aacaatatta tttcattgca
    16261 acaaggcgat agtgagcaac ttcaatttga aaatcaaact tttgatgcag tgatggtagc
    16321 ttttggtgtt cgtaattttc aaaatctgga aaaaggattg atggaaatga aacgggtact
    16381 taatagtaat ggaaaggttt ttattctgga attcagtaaa ccaacaatgt ttcctattaa
    16441 acaattttat ggcatctatt cacgttatct gttaccactt ttcggcaagt tgatttccgg
    16501 tgatgcatcc gcctatacct atttgcctga atcggtaaaa cattttcctt caggaaatga
    16561 ttttgcactt gttttgaaac agtgtggttt tactaatatt gaaattaagc cgatgacttt
    16621 cggaatagta acattataca ttgcagcagc atgaaacaga gggttatagt tttttctttc
    16681 ttagttctgc tgttcaatgc tgatagcttg tttgcacaaa agcaaaaaat tctaaacctg
    16741 ccaaagtatg acaggcagcg ctttcatttt ggatttatac ttggcatcaa taaaacagat
    16801 tttgtaattg acagagttgc ttcttttagt ttgattgatt cactttatac tgttgaatca
    16861 aaaggtacat ccggttttaa tcttcagata gtaaccaaca tgcgaatggg agagcatttt
    16921 gacttacgat ttttgcccgg actggcattt gcatcacgca atttgattta tacatttgaa
    16981 aaaggaagac cactcagcaa cgttgtgacc aaaaaggtag agtcaacttt tgtagagttg
    17041 cctctggatg taaagttcaa gtcggcacga ctgaataatt tccgtgccta tgtattagga
    17101 ggagttaagt acagcataga tatggtatcg caggcgaaag taaaagcaaa ggataaagaa
    17161 tatgtaaagt tgcaacgaaa cgattatgga tatgaaattg gagtaggatg tgatttttat
    17221 ttgcctatgt ttaagttttc accggagata aagatgtatc atggtttacg aaatcttttg
    17281 gttaaagacg atttggttta ctcaaaatct cttgatggat tgttttcaaa aatgttcacc
    17341 ttgtcattga cattcgaata atagaagata ctttttatgc aaacatttga acttgttgtt
    17401 ctgcccgaag aggcagccag tgagtcggta attcaacaca tagttgctaa gaaatttggt
    17461 ggcaacagat tgttccgtat tataaaacgc agtattgatg cccgctctaa gactgtaaaa
    17521 atcagattgc agttacaatg gacagatgaa cagaatcaag aaactttagt tgaaaatcga
    17581 aagtggcggc aagttgccaa tagtgaacct gtaataattg caggatgtgg gcctgcagga
    17641 ttgtttgcag ccatccgact tattgaatgt ggatttaaac caataataat tgaacgaggc
    17701 aaagacgtac gtaatcgcag acgtgacctt gctgcaataa ataaacaaag gcagattaat
    17761 ccggagtcaa attattgttt tggcgaaggt ggtgcaggca cctacagcga tggaaaactt
    17821 tatacacgtt ccaataagag aggcaatatt gcttctgtat taaatacatt tattgagcat
    17881 ggtgccgatg gtgatattgc tgttgatgca catccacata ttggaaccaa taaacttcct
    17941 gcaataattt ctgcaatgcg aaacagaatt gttgaatgtg gaggcgaagt attattcgat
    18001 acaaaacttg taaatattga ttttgacaca agtggtgtaa atggagtaga ggtgcaacaa
    18061 gggttggcac aatttcatat taaatctaaa gctctgattt tagcaaccgg tcattctgca
    18121 cgcgatgttt tttacatgtt aaacaatcaa aaaatattga ttgaagcaaa accaattgct
    18181 attggtgttc gtgctgaaca tcagcagcag gttattgact ctgtgcagta tcattgcagt
    18241 aaacgcagtc aatttttgcc accggcatct tatgcattaa aacatcagca tgatggtcga
    18301 ggaatttatt ctttctgcat gtgtccggga ggcatcattg cacctgctgc aaccgacatt
    18361 ggcgaagtag ttgttaatgg ttggagtcct tcaagaagaa ataatgtttt tgcaaattca
    18421 ggaatcgttg ttagtattac agaagaagat gtattaccat ttaagaatgc aaaagaatta
    18481 cgttttttag aattgcagaa aagtatagaa gaaaaagcat ttgttagtgg tggaagtaat
    18541 cttgctgcac ctgcacaacg tatcgttgat tttgtgaatc aaagagtgtc ttcaaatctg
    18601 cctacttgct cctatatacc gggaatcaat tcagtaaatc tgaaagaaat ttttcctgct
    18661 tcggttacaa actcgcttgc tgcagcattt gttgcgtttg gtaaaaaaat gaaaggctac
    18721 tatacaaacg aagcagtatt ggtgggcaca gaatcacgga cttcatcacc ggttcgtatt
    18781 cctcgtgatg ttgaaacagc aatgcatgtt caggttaagg gactttttcc ttgtggtgaa
    18841 ggtgcaggct atgccggagg cattgtgtct gccgctatgg atggcgaatt ttgtgcagaa
    18901 aaatgtgctg catatctaaa aaataataat tgaattagtt acttttaaat ccacattctt
    18961 taacaatgga aatcaatcaa ccaccttact atatcagaat aacttttaaa atcctgttag
    19021 cgattttaat tttttatgtc ctgcatgctg cacaagatgt actcattcca cttactgttg
    19081 ctgtattgtt tacctttttg ttaatgcctg tttcaaacaa acttataaaa tggaaatttc
    19141 cacctgccat agcaatttta tgcagcattt tacttgcatt cgtagccgtt ggtggacttt
    19201 tgtatttctt ctattcgcaa attgtttcat ttgctgaaga tttgccttta cttcaggcaa
    19261 aactccttga aaaagtagag tttttgcagc gcttcgtaaa cgaaacattc agaattacca
    19321 aagagcaaca aaatgttttg gtaaaatcta aattgcagga aaatattgca gatagtggat
    19381 tactgttgct caatcttttt tcttcaacag gaacttttat tgccaatttt gccttagtaa
    19441 ctgtttatgt tttttttctg acactttata agaaaaagtt taagcagttt attgaagcaa
    19501 tcgtttctga tggaaaacac aattcctatt ttcagattgt aaaaaaagta gctggtgttt
    19561 cacaaaacta tcttaaaggt atttttttag atgttttgat tttatccatt cttaattcaa
    19621 caggattttt gttattgggc attgaacatg ctgttttatt tggagtactg gcttctgttc
    19681 tcaacatcat accctacatt ggtgttttaa taggaagtgt tttaccggta ctaatggcat
    19741 tgctcacaaa agatgaaata ggctatgcaa ttggagctgc cggagtttgt gtcactgtgc
    19801 agtttattga caataatttt attactcctt atgtagttgg cagtagtgta agtataaatc
    19861 cactcactgc aacaatagcg ttaattatca gtgcttcagt gtggggcttg gcaggaatgg
    19921 ttttatgcct gcctctgaca gggatgttaa aggtagtttg cgaccatgta gaaggtttaa
    19981 aacctattgg atttttgata ggtgaggagg tggattatag gaagaataaa tttaaacttg
    20041 ggcgaaaata ggtctgttga aatgaaatgc taattttgca atttatagcg aattggcatg
    20101 agccgattca gattaaaatc aagagtaaaa atggagaatc ttaaacagca tattgaagca
    20161 ttaattttca cttcagaaca aagtatcact gcaaatgaaa ttgctgctgc tttgaaagca
    20221 agtttcggat gggagctttc taatgaaaat ataagacaaa gtatttctga attaatcaac
    20281 aaatattctg ctgatgatta ttcttttgag ttggtagaaa ttgccggtgg atttcgtttt
    20341 ctaagtaaga aggaatatta cggtgtagtg aacaccatgt tgcagataaa ctctaaaaag
    20401 cgacttacaa cagcagcatt ggaaacgctt gctatagttg catacaagca acctgtttca
    20461 aaatcagaaa ttgaaaacat cagaggcgtt aactgcgatt attccattca gaaattatta
    20521 gagaaagatt taattaccat tcagggaaaa ggtgatggcc ccggaaaacc aattttgtat
    20581 ggtatcagcc gaacattcat ggatcatttc ggtttaaaat cagcaaaaga tttaccaaag
    20641 ttaaaagatg taattcttga tgagaacgag attggaacac ctgccgatat gcagtatgca
    20701 gagaacgaca atgccgaagg tgtaagtatt accttaggaa cacaggaagg caatacagac
    20761 aagaccgatg acgttgcaga ataatcagtc tttaattgaa attcattttt tgaggtgaaa
    20821 atttctgtac ttggcttgca atttgattcg gcattaaaaa atgttttcag catgttatca
    20881 attgcactca tttgtttaat gcaaccaagt atcagtgaag gtcaggagaa attacgtaag
    20941 gtagaaaatc aggcatttac ttatggcgaa gttttggaat atcgagtaca ttacggaata
    21001 atagatgcag gcgaagcaaa gcttgctgta atgcctgata aaaaaactat aggtcctcgg
    21061 gaagtgtatc atgtagttgg aacaggacaa acaaaaggag cattcgactg gttttttaaa
    21121 gtgcgagacc gttatgaaag ttttatagat gcacaggcaa ttatcccttg gttgtttgtc
    21181 agaagggttt atgagggtgg atataaaatc aatcaggatg ttacttttaa tcaggtaaaa
    21241 aatttagcca taagcaagaa ggcaaccatt gccacaccac agaatgttca ggatttgatt
    21301 tctgcatttt attatgcacg cacaattgac ttcagcaatg cccaacccgg tgccttatac
    21361 gatgttgatg cgtatttaga tgacgccatt attcctgtaa aattcaaata tgtaggaaaa
    21421 gaaaatatca gtactaagtt tggcactttc cgttgtttga agtttcaccc gcaattgttg
    21481 gaaggccgtg tttttaaaaa caaggaagac atgactctct gggtaagtga tgatgagaat
    21541 aaaattccta tacgagcaga agcaaaaatt ttagttggat ctgttaaaat ggacattaaa
    21601 agcttttcag gacttgccaa cgaaacacta tcgttgttga agaaatagtt taataacatt
    21661 tcgaactaac tttaataaat atctaagtgt gatttgttga gagaagttaa tttatttgat
    21721 aatcagccac ggctgacagg tttgatgatt agataatttg ataatgtttt tgaatttttg
    21781 aatgatgttg gtttccacag agtattcaga tgtaaaattg tagagacaga gatgcggaaa
    21841 aaaagtgttt gttaaattac acaatctggg gttttgcaac ttgacagtta taaagttaac
    21901 tacttgacaa aaggaagaaa ttgttagtcg gtgtttatgt acaaaggtga attcccactc
    21961 agtttactaa gttgctaact tttgtaaact aaaacatatt agctgaaatt actagctttc
    22021 attgctttat cactttacct gttttaaaca cacccttgta gttgtgcaac gcataataat
    22081 aaataccttc tggaagctgc tctgtattta tagtcgtgga gtttgaaaat gtctgttgca
    22141 aaactcgttg tccaacaaag ttataaagag acgctgttgt tttttcgttg ctggcaagag
    22201 aaaaagtaag ttgactggaa aagggatttg gatataaact tacatcagca tttaaaacct
    22261 gaagagtatt ttgattttga aaagatggaa cagacgacac taaagggcat aaactgctgt
    22321 cagtaaatga aattgagttt actgacattt gtgccagcac atcaatactt acaaggctta
    22381 ccggttgaga taaaatgctt gtggcactat cggttaccga atacgaataa gttgtaaagg
    22441 tggatggtgt gctcatacaa ggtgttgtta gaacgctatc agttttgcaa aagtaaaatc
    22501 caaaattatt gaagccacct ccataaccaa acatcgctaa accggtttgt gggttgaagt
    22561 aatggctgta tgaatgacaa tttgagtaat tattatactg gtgcatggat aacaggtttc
    22621 ctgcactatc aattcttgct gctgcattta tcgaaggatt aaaaatgtca aaattacctg
    22681 ctgtaacaaa agtgttgcct gaatccggtg ttatttggtc gaagaaaaga ttgtaaaaag
    22741 tggaatctgt gaaaccaaca ttccatagcg catttccgac actatccgtt tttaaaaact
    22801 gtgcagagcc gttaaaggcg gttgctacgg caaacataaa gccatggtca tgggtttcta
    22861 ctacggaaga aggaataata ttttattgac ataattaagc tggcgttgcc atgtaatatt
    22921 gccaatactg tcggttttga taagtatggt tgaagcgggt aatattgtgc cataaatttg
    22981 ccgggttcct gcaaggagaa aaccaccatc gctggttaga gttaatgccc taagctcaat
    23041 tctgcgtgat gtacctccgt tcatcattct cgaccaaata atgttgccat ctaaatccat
    23101 tttacagaat agccctccat taactccgtt tgctgaaaca cgtcctactc cataaatagc
    23161 attatttttt tcttcctgaa aataaacagt tgatgaaatg tagttaagtt ttttggcacc
    23221 taacatggta cctgaataac ttgttttaag ttgaatagtg cggccatcgt catagaggtt
    23281 aagcattaag gtgctatctg aaagaggtag aacagatagc ccgaatgttt taaaaattgc
    23341 ggtaactctt tgataagatt tactccattt cagtatgccg tcattaccca caagagaaag
    23401 tattgcaccc gaatctggac ctccaaaagt actgagagta taataccccg aatcatcggg
    23461 cgattttata acatcataat aagtgagaaa aatgtcagaa acaccactgg gtttgaacat
    23521 tttttcgaaa gccgtctgtc cgctggacaa gtatgttgca agaataaaac tgaattgtaa
    23581 aagttttttc attgtctaaa ttttcgataa acgattcact gtagtggtgt gttttaacac
    23641 tgtcagtgta actcacaaat ttacgaagtt tgatttttaa aaccaagcaa acgatactac
    23701 ttttcaatct tatagcatct caaagtttta taaaaatagt gcataattat atttttaatt
    23761 tttgaggata taaagaaagt ttgaaggtta taatgttttt aaagtttgtg ttatttctac
    23821 agagtttcgc gaagtaatat cgaagagaat ttgaggggat aaaaattaag tatatgtttc
    23881 tgttaattca ctctgtcagg ggctgtgcta cttgacaggt gaaatcagaa ggttgcaatg
    23941 ttggaaggtt tagaatgttg tttgataagt aaaaaaaaca gtctttgagc gacaaacttt
    24001 tggtggaaaa atagatatca aaaactttta aagtgccata ggtacaacct tattattggt
    24061 aattaaataa cacaatagta atataagttt tttaatgata attaagcaca agtcgaaaga
    24121 cttgagccag atgggagaaa taaattactc aacaattaat ttcttgcagt tgctattttt
    24181 tccatcaaac actttcatga aataaattcc atttgatata ttattcaaat tgataacttt
    24241 ttttgattca cttataatat tttcttcaat aatcttattg ccgaaagtat tgaacatctg
    24301 aattttccca ttgtcaatta catgattgaa ttcaattaca acctgactta atgcagggtt
    24361 ggggtaaatg ttgatggaag aataataatt tgtgttttct gtaatatccg tcaataagtt
    24421 tgaattgaga aaaactccat tcccttgtgt agcagcaaaa aaatttattc cgcttattgt
    24481 taaatccgta acaattttat ttgttaggcc attattcaca gctgaccatg tagtgccgtt
    24541 gttgttagaa aaatacacac catcttgcat tgtggctgct aaaatatttg ttccattaat
    24601 cgcaagacaa gtaataattg atgtggttgg taaaccatta tttactgcat tccacgagtt
    24661 gccattattg gatgaaaaga aaacaccatt actatcagta cctgcaaaaa tatttgtccc
    24721 gctaatagct aaagaagaaa catgcagatt tgttaaacca ttattaaccg cattccatgt
    24781 attgcctgta tcagttgaaa gaaaaacacc gttttgataa gttcctaaaa aaatgtttga
    24841 gccacttatt actaaacttg aagagccgcc tgttggtaaa ccattgttta tcagattcca
    24901 tgttgaacca ttattaacag ataaatagac ttcatttaaa taagatgcag caataagatg
    24961 agaacctaat ttagcaacag ctattataaa tttatttgta ataccactat cggattgaac
    25021 ccaagtattt ccagtgtcag cagacataaa aagtccgtta actgttccaa caaaaaggtc
    25081 attagtagta gctaggatag aattaataaa agtgctggtt aaaccattat taatctggct
    25141 ccaagaattt ccattattag tagagcgata cattccctta taattacttc ctgcaaataa
    25201 atttgaccct gacacagcta atgaatgtac gaaagaactt gccaagcctg tattttgtgc
    25261 attccaggaa gcaccattat tagttgatag aaataaacca tctgcagaag tgccaacaaa
    25321 gatgtctgaa ttatttataa gaaatgaata aattgttgaa cttgttaagc cattatttgc
    25381 cgaagtccat gtatttcctg tgtcagatga aaaaaataca cctcctccgg cagttcctgc
    25441 aaaaacattt gcaccacttg ctgcgattgc agaaacgtgc agatttgtta aaccattatt
    25501 taaggcattc catgtatttc cagtatcagt cgatagaaaa acaccgccat agattgaacc
    25561 tgcaaaaata tttccagcat ctattgctaa atgactaata tgtaaatccg tcaaacctgt
    25621 attaacagct acccatgaaa caccattgtt ggttgataaa taaacacctt gaaattgtgt
    25681 tacagcaaaa atattatttc cacttattgc aattgagttt atttgaatta atggtagccc
    25741 tgaattagcc accgaccaag aaactccatt attcgttgac gtatacacgc cattatctga
    25801 agtgccaata aaaaggtttg ttgcatttgc ggcaaatgtt gtaacagaag tgaatggtgg
    25861 taaaacaata tttattaatg accacgaatt tccattgttg gatgtcacat atagtcctcc
    25921 atctgtaacc gcaaataaat ctgcaccatt taaccaaagt gttccaacac gaatatgtgt
    25981 caagccatta tttatagctg tccacatctg accattgttt gaagacctat atacaccacc
    26041 ataataagta cctgcataaa gatttgttcc atcagttact attgatgata tatccccacc
    26101 ttcaggtcca tttgtctgtg tccactgtgc atttacagtt tcaaaaataa gtaagcaaaa
    26161 aataattgca atcagatttt tcattatatt ttgttttaca cagtttacaa aaattaatta
    26221 cttgcaatca acatgaaatt acactgccgc taaacaatat cagataacat cattcgggcc
    26281 gatagttatc aatccaatga ctatcagaat taaaactatt cattagttca aaagtagtaa
    26341 aaaaactatt taaaaaaaca atagcgaggt gttaggcaat ttaataagta tgaagtgcca
    26401 atgtgatagt atatacacac aggtttgcta cttaactttt tctatactac ctatcagcag
    26461 acatgcgcga aaattttaaa gggtttgcag ttatttcgct tgtgatttgt cgttgttttg
    26521 caatgtcgca ggcaagatag acagcctcac ggaatgaatc ttctgatgcc tgattttttc
    26581 ctgccagatc gtaggcagtg ccatggtcgg gcgaggtgcg gatgattgga agacctgctg
    26641 tgtagttgac ccctgttgag aatgccaatg ctttgaatgg taccaatccc tgatcgtgat
    26701 agatggccat gatggcatcg aaacttttaa agcgcgaaga tccaaaaaaa ccatcggcag
    26761 ggtagggtcc aaaggcacca atgcctgcat tgtacatttc actgatggca ggggtgatga
    26821 tttcctgttc ttctttaccc attaaaccat tatcgccagc atgtgggttg agtgcaagaa
    26881 tggcaatacg tggcttttgt aaaccaaagt cgctgcgaag tgactgatgt accaatttgg
    26941 tctttgcaat aattttttct ttggtaagct gtgcaggcac atcctttaaa gctacgtgac
    27001 ctgtgatgag tccaacacga agtttgtcgc tcaccataag cataacgtgt tcggcattgc
    27061 cgcagagctt tgcaaaatat tctgtatgcc ctttgaaagg aaatgcatcg ctgtgaatgg
    27121 tgcttttgtc aatgggcgct gtaactatgg catcaatttt tttatcgagc acatctttgc
    27181 ctgcagcttc gagtgatttg aatgcatagg ttcccgcaat ggttgatggt tttcccaatt
    27241 caactttaac atcttcttcc cagcagttga tgacattggt ctttttgtga cttgcttctt
    27301 cggcagtacg gatggtgttg aaattaaatt cctgctgatg cagtgatttt ctatgatagg
    27361 aaataacttt tgaagaacca tagatgacag gtgtacatac agagagcatt ctgctgtcgg
    27421 caaaagttt
//
LOCUS       DAOOOI010000022        26245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_333_length_26245_cov_29.005346, whole genome shotgun sequence.
ACCESSION   DAOOOI010000022 DAOOOI010000000
VERSION     DAOOOI010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 26245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26245
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_333_length_26245_cov_29.005346"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..721
                     /locus_tag="PKX59_04670"
     CDS             197..721
                     /locus_tag="PKX59_04670"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ90194.1"
                     /translation="MMNDRIKILKANLNDTTALSVIAATTFYDTYGAYNKQETIQRYI
                     DKWFSTSQMCKELSDDRIIYYLVYDQQQLSGYIKLQFVNPHQNSDLNGVELSRLYLLK
                     QWHGSGVSEVMLNHCYKIAEELKQDAIWLSVWEKNNRAIRFYEKHGFVKKGNLIFKMD
                     DEDQNDFLMVKHLI"
     gene            complement(846..1736)
                     /locus_tag="PKX59_04675"
     CDS             complement(846..1736)
                     /locus_tag="PKX59_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOZ90195.1"
                     /translation="MTENQKPNNQELHAMISLLDDEDETVYESLLNHFLEIGKPAIPL
                     LEEKWSQSLDALLQSRIENIVHKIQFESVKSELKNWKEHNQKNLLAGLITVARYQYPD
                     LDEDAIRAFFSEVRRRVWDDLQPNDTPLEQVRILNQVLFVEFGFNGNTANFHSPQNSF
                     INTVIESKRGNPILLSVIYSIVAQDLDIPIYGVNLPEHFVLCYQTLNKDKFAEYAFPE
                     ENILFFINAFSRGSVFGKNEIDLYLKKLRLQPDKKFITPCSNTDIVRRILHNLNFSFN
                     KSGLTEKSNEIETLLSLLEI"
     gene            1884..3017
                     /locus_tag="PKX59_04680"
     CDS             1884..3017
                     /locus_tag="PKX59_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOZ90196.1"
                     /translation="MKGQETFFISCAPHVVPWLKQELDDLGYMPFEENYTGLFIKAPF
                     EKCMELNFQLTTASRVLWLVDKFTATDQKQLYNKLSRIRWSDYFSEKKYITITSQTNH
                     PSINNSMFLNQLCKDAVVDFFQSHYNIRPDSGPLRSEVVIHLHWQENKAWVYLDTSGE
                     QLNKHGYRQNPFKAPVQENLAAAIIKATAWDKKCTFINPMCGSGTFAIEAAMMAGNKL
                     PLLSRSNFGYMHLFDFNKKKFELLRSELALKSNFENMPAIIASDNNGYAIKATRENAE
                     RAGVDAFIEMTKCDFTETSIPDGNGVIVINPPYGERLGENIDLEALYKSIGDFFKQQC
                     KGKTGFIFTGDLNLAKKVGLRTSARIPFYNGTIECRLLKYDLY"
     gene            complement(3020..3637)
                     /locus_tag="PKX59_04685"
     CDS             complement(3020..3637)
                     /locus_tag="PKX59_04685"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOZ90197.1"
                     /translation="MFETSYKTIVGYSEGVFRDKGSRFLVFAYYVEKESECKSVLEQI
                     KKEHPKANHHCYAYRLGPYKNIFRWNDDREPSNTAGRPIYAVIQSNDLTNILIVVVRY
                     FGGTLLGVPGLIQAYRSAAEATIEQNTIIEKDIEEEFELQMSFEEMAEVMKIVKQHKA
                     TFTQTSTANTLTLVVKAKKTFSDQFLKAINGNYLLKDKVKIRAIS"
     gene            complement(3638..4498)
                     /locus_tag="PKX59_04690"
     CDS             complement(3638..4498)
                     /locus_tag="PKX59_04690"
                     /inference="COORDINATES: protein motif:HMM:NF018506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HOZ90198.1"
                     /translation="MTVTVFMAVLFASLLFVILKLFDRFKVSNIHGLTFNYFTAGICY
                     TLYSKQTYSSISGFAASYFPYYLLSGSLFIGVFYLTALSSQKNGVASTTIASKMSLVI
                     PALLAVFLYNETFSFLKTIGLLMALSAVYLSARGSNNESSESGWTLPLLVFAGAGLVD
                     SSIKYTQHVFTDNAGSSGFFTLVFFSAAIIGSCVLFFSKNMPKEGSLFRNIIAGIILG
                     IFNFISLYYMVAALQEFHEDSGKAFILINSGVVLLNFLLSIILFQEKKNTQSWVGVAL
                     AVVAIIILSQ"
     gene            complement(4495..5526)
                     /gene="ribD"
                     /locus_tag="PKX59_04695"
     CDS             complement(4495..5526)
                     /gene="ribD"
                     /locus_tag="PKX59_04695"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOZ90199.1"
                     /translation="MTFDETIMNRCLELAKKALGQTMPNPMVGSVLVFENKIIGEGYH
                     HRAGEAHAEVNAINSVENKNLIPLSTLYVSLEPCSHYGKTSPCCERIVQEGIKKVVVG
                     TLDTTAKVNGKGISYLQQNNVEVATPMLNDACRFLNRRFFTFHEKQRPYVILKWAESA
                     DGFIAPENAQQQYAISNTYSKTLSHKWRTEEQAILVGTKTAILDNPQLNPRLWKGSTP
                     IRVVIDKNLDVPATHHLLDGSVKTILINQKKENRANNIDYIKINFQENPATQILKVLY
                     QQNIQSVIIEGGAKTLQYFIQSNLWDEARVFTATKQLISGIKAPVFLISPSEITNIED
                     DTLKIFYNV"
     gene            5556..6419
                     /gene="prmC"
                     /locus_tag="PKX59_04700"
     CDS             5556..6419
                     /gene="prmC"
                     /locus_tag="PKX59_04700"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOZ90200.1"
                     /translation="MMRLFSFATMTRKEIINVFQADVELIYDKGESSAITKLVMEYIF
                     GLNYKSNTHENVNVSQETLLRQYLERLRNYEPVQYILGQAWFCNHIYKVNSSVLIPRP
                     ETEELILLIKQNLKSSHIGSIIEIGTGSGCIAIELSLLFAQATVIATDISENALEMAR
                     KNAESLRASVSFINESLFVPSSKTIQTKVDLIVSNPPYITQQEATQMHPNVLQWEPHT
                     ALFVKENPLEFYIAIASLATNILNDKGYVFLELNPDYAFQVAQCFTEKGFTQVEIVKD
                     ISGKERFLKVQ"
     gene            complement(6437..9274)
                     /gene="uvrA"
                     /locus_tag="PKX59_04705"
     CDS             complement(6437..9274)
                     /gene="uvrA"
                     /locus_tag="PKX59_04705"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOZ90201.1"
                     /translation="MQTEHESIEIYGAREHNLKNIDLVLPREKLIVFTGLSGSGKSSL
                     AFDTIYAEGQRRYMESFSAYARQFLGSLERPDVDKINGLSPVISIEQKTTNQNPRSTV
                     GTVTEIYDFMRLLFARAGEAFSYMTGEKMVRLSDDQIVEIILRDFDKKNIVVLAPVVK
                     ARKGHYRELFEQIRVSGFIRVRIDGVITEIKKGMQTDRYKTHDIEIVVDKIEINETAS
                     KRLYDTVKTAMRFGKNTLMIIADDQKRVRFFSRNLMCPTSGISYDEPAPNTFSFNSPY
                     GACPRCNGLGTIPEADLASIIPDNKKSIRKGGILPLGEYKNNWIFGELEYIGRRFGFN
                     LNTPIHDIPHEALNIILYGTDDVSLNATPGSSHGITFEGIVNFIVNQMEENASYSIKK
                     WALNFMQTIICPECEGTRLKKESLAFKLNNKNISELSLMDLGELQQWFQQLPASLSEK
                     QNIIATEVLKEIVKRLGFLLDVGLDYITLNRSTRSLSGGESQRIRLATQIGSQLVGVL
                     YILDEPSIGLHQRDNERLIKALKDLRDIGNTVIVVEHDRETIEAADYVVDLGPGAGIN
                     GGDIVAIGKPEELKYQQSLTANYMSGGIKMPSSKNRKGNGKKLTIAGAKGNNLKNVTA
                     LFPLGTLICVTGVSGSGKSTLVNETIYPALNKHFYNSTQKPLPYEKINGLSHIDKVIE
                     IDQSPIGRTPRSNPATYTGVFTDIRNLFSQLPEAKIRGYAPGVFSFNVSGGRCDTCLG
                     AGLKIIQMNFLPDVHVHCETCNGKRYKREILEVRYRGKSISDVLNLSINEAVDFFSNI
                     PAILHKIKTLQDVGLGYITLGQSSTTLSGGEAQRVKLASELSKKDTGNTFYILDEPTT
                     GLHFEDVRILLLVLQQLVDKGNTVVVIEHNMDIIKAADYIIDLGPEGGMRGGNIVAEG
                     TPLQISKNANSITGTFLKKEMEWHNKVSAEV"
     gene            complement(9400..9915)
                     /locus_tag="PKX59_04710"
     CDS             complement(9400..9915)
                     /locus_tag="PKX59_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90202.1"
                     /translation="MRIWGLKLLVLIWVVFTGYWLLPLKKPKAYRQDSEPNTILATQQ
                     FCEPECADIIIKKGELIIPDSLKKMYPELRKDVALLVGSSPFKKSKSALMFSYDFVLS
                     GHVERVGFTQQEGFVPVFHVEEWFPTQYIARFWKLTHNSELLYLINLNIGLPLLLFYF
                     FKQGSSLNKFF"
     gene            complement(9929..11998)
                     /locus_tag="PKX59_04715"
     CDS             complement(9929..11998)
                     /locus_tag="PKX59_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOZ90203.1"
                     /translation="MKTIQLFFISVLFCNITLAQVVPPQDDSKIEEKVENIANTADES
                     ADLTELTEQLQQLAANPINLNKATREDLLQTGLFSEVQADAIIEHREISGKFISLYEL
                     QAIDFLSLEDIEEILPYVTVGNTEDIKATLKKIFTDGNSRLIVRGQRYLEKQKGFRDS
                     TATDKVHPAYPGSPWKLYTQYQYRYKQRFSFNLTAEKDQGEEFFKGSQRNGFDYYSAH
                     LAIRDFGIIKRAVIGDYDLFYGQGLTLCTGLSFGKSPDVAAVRKFSTGIRPYTSLNES
                     SFKRGVAVSVGKRNWNADAFYSNRKLDANLNSILDTTLEQQDIFTSFIESGYHRTKSE
                     LENKNTIREQMYGVNAGYNTRNFTAGLTAVHAGYDRPLQRSFQLYNRFDFSGKQFTKV
                     GIDYSWLYRNINFFGEFSRSDNGAIAYVNGAIISLGTTVSVSVVNRNYPRDFNFLYAS
                     GFGESSTTRNEKGTYAGIVIRPNRYWGFNGYLDRFTFPWLRFGADAPSKGYEYLWQVS
                     WNPSRQFEIYFRNRRTQKEENSSAAAVADYLVFKRQDNYRFNLTYKISKAVAIRNRIE
                     WVRINYEEKPNEKGFMIYQDILFKPLSSPISGSVRYGLFDTDSYDSRIYAFENDILYS
                     YSIPSFYNRGFRYYVTLRYKVTKGVDVWFRFAQTVYTNQKTFGSGLDEINNNKRSEIK
                     LQVRYQF"
     gene            complement(12001..12813)
                     /locus_tag="PKX59_04720"
     CDS             complement(12001..12813)
                     /locus_tag="PKX59_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90204.1"
                     /translation="MKPTILAILLFCFSSVSAQTYLTSGGARASAMSDAAVTFNDVFA
                     AFNNQACLSMLEKPEAGLAIQNRFGMKELNTLSAAFALPLASNKGVFALSINRYGYKL
                     FNQNKIGLAFAKKLSPVFSAGVQVDYLNTHIDEGYGNKSSFTIEGGVLAEFNKLKAGV
                     HIFNPLHVKLTDYDDERIPVIIKAGISYSFSEKVLAAAEIFKSIDRKETYKIALEYHP
                     VKALYVRGGVSTEPVQLTFGFGLKFSQFYADFTSGYFEPLGYSPGLALRYAF"
     gene            13222..13854
                     /locus_tag="PKX59_04725"
     CDS             13222..13854
                     /locus_tag="PKX59_04725"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90205.1"
                     /translation="MEFWDSGFNGVPSFSGNQFLEINANEVASIYQDVYLTAGDVITL
                     HFAHRARVGIDTLGVSVSFQNNTPVILGYYADDDLVWGYYTEQYTVPATGQYRISFIS
                     VSNFYNVPTLGNFIDAVEVCLSPVGFDEVNLQETFLISSQKSTLKIDFTSQLKGEGIL
                     TVFDLQGKEVFRISVDNSRKQIATAPLNSGIYTVQLIQGRSITAKKVLIN"
     gene            complement(13851..14582)
                     /locus_tag="PKX59_04730"
     CDS             complement(13851..14582)
                     /locus_tag="PKX59_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOZ90206.1"
                     /translation="MSDRISNELEHGKKLLEGGAEAIWNWDSPAGKVRADRRADLIAK
                     YSLADKSSTVLEIGCGTGLFSGKFYQRTGAKIIATDLSPELLNVAKEKYPAVEFVLAD
                     AMKLQFDANTFDVVFGSSVLHHLEFERSLNEILRVLKPGGRMVFAEPNMINPQIFVQK
                     NIPFIKKWLGDSPDETAINRWNFSKLLEQKGFNQINIFPYDFLHPVVPSFLISLVSGI
                     GQVVEKIPIAKEIAGSVIIVAEKPR"
     gene            complement(14582..16249)
                     /locus_tag="PKX59_04735"
     CDS             complement(14582..16249)
                     /locus_tag="PKX59_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90207.1"
                     /translation="MKKNKEVVAKKSSTKTVNKPSRETTLIKLENFLQKNETIIFFSL
                     LLVSLIFSVLLFDIKMSEGNDDSDYIEGAFKLTKDFSSYYSTKAPLYPIFLSLPVGIW
                     GINLPILKSLSILFSLLNLTAMYYAFRKLIPYTILFAVLIFTGINSYFLYFASQTYSE
                     AFFCFLQALLFIVIRPILSASKEDNIPIAKYLVFGIILTLLSLTKNITVGAIAAVALY
                     FVLNRDFKKLAYAFGSFVVVRGIFEVIKSFIWGSQNQFSNQYQILIQKDPYNASKGVD
                     DFGGFVDRLFSNTSIYIGKRLYQILGFVSPDNTKTNAGLVLITFVFVGIGLYFIFKNK
                     NKIMQFTFLYVLLMAGLTFFVLQTSWDQPRMVLVYVPFILMIIFYGLYYAFQNTPALR
                     SSIFLIVSIIIIMSSLMKTVDKSVTNLPILKKNMGGDIYYGYTEDWTNFLKLSRYCGD
                     SLPESAYVVSRKAPMSFIYSKGKSFYPIYTVPSTDPDSVLALLKENKVTHVLIASLRR
                     NPKKNDGYVINTLHRMMGPVGQKYPDKLKLVKQVGTSEPSYLYEIKY"
     gene            complement(16264..16917)
                     /gene="fsa"
                     /locus_tag="PKX59_04740"
     CDS             complement(16264..16917)
                     /gene="fsa"
                     /locus_tag="PKX59_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOZ90208.1"
                     /translation="MKFFIDTANLAQIKEANDLGVLDGVTTNPSLMAKEGIKGKAAVM
                     KHYVDICNIVKGDISAEVIATNYDGIVAEGEELAKLHEQIIVKVPMIKDGIKAIHYFT
                     SKGIKTNCTLVFSAGQALLAAKAGATYVSPFIGRLDDVSTDGLQLISEIRQIYDNYGF
                     QTQILAASIRHAMHIINCAKLGADVATCPLNAITDLLKHPLTDIGLEKFLADAKKFV"
     gene            complement(16942..17511)
                     /locus_tag="PKX59_04745"
     CDS             complement(16942..17511)
                     /locus_tag="PKX59_04745"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOZ90209.1"
                     /translation="MLNIVLFGPPGAGKGTQSEKLIQQYGLIHLSTGDLLRNEIAGQT
                     KLGLEAKKLMDQGVLVPDEVVIGMIESKLDANPTAKGFIFDGFPRTTAQAAALDNLLK
                     QKNTSIKMMLALEVTEAELSKRLLLRGKDSGRPDDQNPEIIKKRINEYNNKTAPVAEY
                     YRMQKKYYGINGIGSIDEIFSLLCKAIDK"
     gene            complement(17571..18107)
                     /gene="hpt"
                     /locus_tag="PKX59_04750"
     CDS             complement(17571..18107)
                     /gene="hpt"
                     /locus_tag="PKX59_04750"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOZ90210.1"
                     /translation="MSEVTILDKTFTIMIPRKQIETRILEIARDLNAEYKDKNPVFIG
                     VLNGAFLFMADLFRNIETPCEISFIRVSSYSGTESTGQMKNVVGLSGNINNRHVIIVE
                     DIVDTGETVSYLMQEIAKHQPASVKLATLLFKPQALKKKVTVDYAGFEIPPAFVVGYG
                     LDYDGYGRNLQDIYVLKS"
     gene            18234..19364
                     /locus_tag="PKX59_04755"
     CDS             18234..19364
                     /locus_tag="PKX59_04755"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HOZ90211.1"
                     /translation="MNAFNPNIVIGVLGGGQLGRMLLQKAADYNLSIHILDPDEAAPC
                     RYMCKTFEHGDFRDYQAVMAFGNDCDIITIEIEHVNIEALYELEKLGKKVYPQPHILE
                     MVQDKGLQKEFYQKNNLPTAPFYLVEKPEQIKPEFPFVQKLRKGGYDGKGVLVVKDAD
                     GMSQLFQAPSVIENKVVIEAEIAVITARNNSGEIKSFPVVEMQFNAEANLVELLFSPS
                     KTSAVIQQKAVSIAEDLISKLNMVGLLAVEMFVTKDGQVLINEIAPRPHNSGHHTIEA
                     NHTSQFEQHLRAILNLPLGNSSTILPAAMINLLGDKDSNGRAIYSGVQDALNAGDVHI
                     HLYGKKITKPYRKMGHVTVTAATVDEAILNAQKIQSQLKVIS"
     gene            complement(19357..20175)
                     /locus_tag="PKX59_04760"
     CDS             complement(19357..20175)
                     /locus_tag="PKX59_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90212.1"
                     /translation="MENNKMNKLFDAARNADPVVSLDESLEQFNKEVEGKETTLGYMK
                     WLKPVIAVATIAVVGGGIYFLVQNQGQQKTQPTQENTEQTTQPALQATETQPDAAIEQ
                     PQATEENLTETPSVKTEITNPAPLNNKVTHAASANDAYIVETQTVNINNDNGAFKISI
                     SGDDVERIVLNGKEVEKSDWSKYQDEVAEAMAAKSSMGINKENAANKKFVDYMFTTLK
                     ENGLIKNNLAVIKLSNDMLLIEGQEADSAIHQQLLKEYKKITGKDIGLRTLYFN"
     gene            complement(20197..20715)
                     /locus_tag="PKX59_04765"
     CDS             complement(20197..20715)
                     /locus_tag="PKX59_04765"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOZ90213.1"
                     /translation="MSGQEKISGKQKAFTDAYQQLHEKLVRFVQSMVYNREDVKDIVA
                     ETMLVAYEKFETIRHKDALLSYLFTVSSRLVYKLQDKKKQIYTAFDPEQAEQVSDDHA
                     GADSKVQLKELYNALDQLPLKQKEAIVLFEISGLSLAEIQQIQGDSLSAVKSRIARAR
                     ESLKKILEEEIV"
     gene            20825..21772
                     /locus_tag="PKX59_04770"
     CDS             20825..21772
                     /locus_tag="PKX59_04770"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOZ90214.1"
                     /translation="MQALSSTNFKFQGQTAFYKGKVRDVYSIDNKWLVMVVTDRVSAF
                     DVVLPRPIPYKGAVLNQIAAHFMQATADLVPNWIVDTPDVQVSIGIPCQPLRVEMVIR
                     GYLSGHAWREYKQGKRMLCGVKLPDGLNESDALPEPIITPTTKAAHGHDQDISKEEII
                     KQGIVDAAIYEQAESYTKQLFRRGSEMAAQRGLILVDTKYEFGVHQGKVYLIDEIHTP
                     DSSRYFYADGYAERQKRKQPQKQLSKEFLRQWLIENNFQGLAGQTVPEMSDEIVQMVS
                     DRYIELFENITGKTFVKPSNENIEQRIYGNIVKSLEKLT"
     gene            21919..22515
                     /locus_tag="PKX59_04775"
     CDS             21919..22515
                     /locus_tag="PKX59_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90215.1"
                     /translation="MKKQIASLLFIAAVVSTASAQKIKILDGSLAGLKGETKLNVVYD
                     YSNMGVGSFAKEEEYVAKKTREYNAKEPGRGDSWAKSWVADRKNRFEPSFVELFNKKS
                     PMLIGDFPDAKYTMKVSTTFTEPGFNVYVTRKNASINLEITVYETANPDKPICKMQGL
                     RFPGRTFGGYDYDTGTRISESYAKAGKDLAAFIGKNLK"
     gene            complement(22590..23705)
                     /locus_tag="PKX59_04780"
     CDS             complement(22590..23705)
                     /locus_tag="PKX59_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOZ90216.1"
                     /translation="MLIDVLHQRILGNTIEDYLWFAGLILAGLVFKNLISRILTFLLF
                     NVLKKYFKTIGVNKFVELTQKPFSATLFLIIIYIACRYVHFPDEWKLASESEFGVRMT
                     IKLIFQIVTVFFITWTILRVIDYIGLVMRERANKTESKMDDLFVPFFASSMKAITVII
                     SLFFILGAVFKLNVASIIAGLGIGGLAIALASKDTLENLLGSFLIFMDKPFVVGDLVK
                     TDSLRGHIEEVGFRSTKVRTIEKTLVIVPNKKMMDAELENQTERTFYRHRFVIGLVYS
                     TTADQIQNIIQQILNMLNEHPLVNENPNVFFTNFGASALEIEVIYFVKTQELSDFENT
                     RQEVNFGVMKIVAENKSDFAFPSTSVYIEKEVKKFLQ"
     gene            complement(23705..24931)
                     /locus_tag="PKX59_04785"
     CDS             complement(23705..24931)
                     /locus_tag="PKX59_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HOZ90217.1"
                     /translation="MLDLLKKFEEKRPEIVFEWSDAETGAEGWLVINSLRNGSAGGGT
                     RMRKGCDKREVESLAKTMEVKFTVSGPPIGGAKSGINFDPADPRKNQVLERWFKAIKP
                     LLKTYYGTGGDLNVDEIHDVIPICNKLGILHPQEGTAVGHFNDGETNIKNRISNLQQG
                     VSLVLKDASLSPDLSHKYRVADMITGYGVASAVKYYYELWGGKISEKRAAIQGWGNVA
                     AAAGFYLAQMGVKIVGIIDRDGGLINENGYTFEEIKTLFNNRQGNKLVADNMLSPQQM
                     AEKVWNLDAEIFIPGAASRLVTQEQMQSLINKKLEVISCGANVPFADKEIFFGPIGLY
                     CDKHVSVIPDFIANCGMARVFAYLHNANAVIDDRLIFDDAATTIKNALQEVIATHKDK
                     KGIASAAFEIALKKLL"
     gene            25065..25247
                     /locus_tag="PKX59_04790"
     CDS             25065..25247
                     /locus_tag="PKX59_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90218.1"
                     /translation="MRNQRFEQKKSIKDHWETDGHHAKKQKMANHREEKNWKNKILKE
                     EDDDSDEDFFRYDEDE"
     gene            complement(25284..>26245)
                     /locus_tag="PKX59_04795"
     CDS             complement(25284..>26245)
                     /locus_tag="PKX59_04795"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOZ90219.1"
                     /translation="AAETIEYDQKRRDELANANLLSGEKLFELHHKFGVFLGESSLNF
                     LQKHQLSCDLIASHGHTVFHQPANGFTTQIGHGADIAAIAGITTVCDFRSLDVASGGQ
                     GAPLVPIGDELFFGMYDYCLNLGGIANISCKVKNSRIAFDICPVNMALNYLANSGGLK
                     YDAEGKLAAAGKLNTTLFDKLNALEFYRKPYPRSLGAEWFNEYFLPIINSSTGTLNDK
                     LHTITQHIAYQISQALTLAGNNPNTTALITGGGAKNKFLIQKIQEKTTVKIIVPDVKI
                     IDFKEALIFALLGYLRINNNKNTLASVTGAKKDSVGGAVYLMT"
BASE COUNT         8271 a         4752 c         4677 g         8545 t
ORIGIN      
        1 agacacatgc caatgcagca ggacaaaaca cattacaact agacctgagc aaggtagcta
       61 aaggagcgta catgttgaat gtacaaacca atggcagcaa caatcaggtg agagtggtag
      121 tggagtagtt tttggttaaa taaattgcta aggggcagtg cgaaagcact gccccttatt
      181 tttgccttat actttaatga tgaatgatag aataaaaata ttaaaggcaa atttgaatga
      241 tacaacagca ttatcagtga tagctgcaac tactttttat gatacttatg gtgcttacaa
      301 taagcaggaa acgatacaac gttatataga taaatggttc agcacttcgc aaatgtgtaa
      361 ggagttatca gatgatagaa taatttatta tcttgtttac gatcaacaac aactttccgg
      421 ctatataaaa ttacaatttg taaacccaca tcaaaatagt gatttaaatg gcgtggagtt
      481 gagcagactt tatttgttga aacaatggca tggcagtggc gtttccgaag tcatgttgaa
      541 tcattgctat aagattgctg aagagttgaa gcaggatgca atttggttgt ctgtgtggga
      601 aaagaataat agagcaatac gtttttatga aaaacatgga tttgttaaga aaggcaatct
      661 gatatttaag atggatgatg aagaccaaaa tgatttttta atggtaaaac atctgattta
      721 aaatgtaata ttccttcgta ataaatagtt tctgcgcaaa ctgaagatat atattgtaat
      781 tgaataggtt tattgtaaat acatattatt tgaattaacc gcttcctact ggcaggaagc
      841 ggtttttata tttctaataa agagagaaga gtttctattt catttgattt ctctgtcaga
      901 cccgatttgt tgaatgaaaa atttaaattg tgtaatattc gtctgacgat atctgtatta
      961 ctgcaaggtg taataaattt tttatcaggc tgaagtctta attttttcag gtatagatca
     1021 atttcatttt taccgaatac agagcctctg ctgaacgcat tgataaaaaa caaaatattt
     1081 tcttccggaa aagcatactc agcaaattta tctttattta atgtttgata acacaataca
     1141 aaatgctccg gtaggtttac accataaatg ggaatgtcaa gatcctgagc aacgatagaa
     1201 taaattacgc tcagtaaaat aggattgcct cttttgcttt caataactgt attgataaat
     1261 gagttctgag gtgagtgaaa attggctgtg ttgccattaa aaccaaattc tacgaataga
     1321 acctgattca gaattctgac ttgttctaaa ggcgtgtcgt taggttgaag atcatcccaa
     1381 acacgtcttc ttacttcgct aaaaaatgct ctgatggcgt cctcgtctaa gtcgggatat
     1441 tgataacgtg caacagttat taaaccggca agtaagtttt tttggttatg ttctttccag
     1501 ttttttaatt ctgattttac agattcaaac tgaattttat gtacaatgtt ttcaattcgc
     1561 gattgtaaca gggcatcaag tgactgactc catttttctt ctaacaatgg aatagcaggt
     1621 ttgcctatct ccaaaaaatg attcagaagc gattcataaa ccgtttcatc ttcatcgtca
     1681 agaagactaa tcatggcatg caactcctga ttgtttggtt tttgattttc tgtcattagt
     1741 gcaaagttaa gccagagcga atacaatatt acaacgcaga aatgaaatgt ttaatatttt
     1801 aagcaacgtt tttaacatca catgttaaac tttacctatg ctgatgatac taaaacctac
     1861 ctttgcggca aaataaattt tcaatgaaag gtcaggagac gttttttatc agttgtgcgc
     1921 cacatgttgt accctggtta aagcaggaat tggatgactt aggttacatg ccttttgagg
     1981 aaaactatac agggttattt attaaagcac cctttgaaaa gtgtatggag ctaaactttc
     2041 aactcaccac agccagcagg gtactttggc tagttgataa atttactgca acagatcaga
     2101 agcaacttta taataaactt agccgcatca gatggtctga ttatttttca gaaaaaaagt
     2161 acatcaccat tacttcgcaa actaatcatc cgtcaataaa caacagtatg tttctgaatc
     2221 agctttgtaa agatgcagtg gttgattttt ttcaaagtca ttataatata cgccccgact
     2281 caggaccatt acgtagtgaa gtggttattc atctgcattg gcaggaaaat aaggcatggg
     2341 tatatctcga cacctccggt gagcaattaa ataaacatgg ttaccgtcag aatccattta
     2401 aagcaccggt gcaagaaaat ctggcagcag caattatcaa agcaacagcg tgggataaaa
     2461 aatgtacatt tattaacccc atgtgtggta gtggcacctt tgcaattgaa gcagcaatga
     2521 tggccggaaa taaattgcca ctattgtcaa gatcaaattt tggatacatg cacttattcg
     2581 attttaataa gaaaaaattt gagttgcttc gtagcgaact tgcattgaaa agtaattttg
     2641 aaaacatgcc tgcaatcatt gcatcggata ataatggata tgcaataaaa gccacacgcg
     2701 aaaatgcaga acgtgccggt gttgatgctt ttattgaaat gactaaatgt gattttacag
     2761 aaacctcaat tcctgatggc aacggtgtaa tagtaattaa tccgccttat ggtgaacggt
     2821 tgggtgagaa tatagatttg gaagctcttt ataaatcaat tggagatttt ttcaagcagc
     2881 aatgtaaagg aaagacaggt tttatcttta cgggcgatct taatcttgct aaaaaggtgg
     2941 gactgcgaac ttcagcacgc attccattct acaatggaac tattgagtgc cgcttgctga
     3001 aatatgattt gtattaaaat caagagattg ctcttatctt aactttatct ttcaaaagat
     3061 aatttccgtt tattgctttt agaaactgat cagaaaaagt tttttttgct tttactacta
     3121 aagttaaagt gtttgcagta gaggtttgtg taaacgttgc tttatgttgt tttactattt
     3181 tcatcacctc tgccatttct tcaaacgaca tttgaagttc gaactcctct tcaatatctt
     3241 tttcaataat tgtattttgt tctattgttg cttctgcagc acttctgtaa gcttgtatca
     3301 agccgggcac accaagtaaa gtaccaccaa aatagcggac aacaacaata agaatatttg
     3361 ttaagtcatt ggattggatt actgcataaa taggacgacc ggcagtgttg gaaggctctc
     3421 tgtcatcatt ccagcgaaaa atatttttat aaggtcctaa acgataagca taacaatgat
     3481 gattggcttt tggatgttct ttttttattt gttcaagaac agacttacat tcactttcct
     3541 tttcaacata gtaggcaaaa actagaaacc tgctgccttt atctctgaaa acgccttcag
     3601 aataaccaac gattgtttta tagctcgttt caaacattta ttgcgacaat ataataattg
     3661 ctacgactgc aagtgcaaca ccaacccaac tttgtgtatt tttcttttct tgaaataaaa
     3721 taattgaaag taaaaagttt aacaatacaa cacctgagtt aattaaaata aatgctttac
     3781 cactgtcctc atgaaattcc tgaagtgcag caaccatata ataaagagaa atgaaattaa
     3841 agatacctaa aataattcct gctatgatat ttcggaaaag gcttccttct ttgggcatat
     3901 ttttactgaa gaaaagaaca caactgccaa ttattgcagc agaaaaaaat actaatgtga
     3961 agaagccact actgcctgca ttgtctgtaa aaacatgttg tgtgtatttt attgaagaat
     4021 caacaagacc ggcacctgca aataccaata atggtaatgt ccatccggat tctgatgatt
     4081 cattgttact gcctcttgct gataaatata ctgctgatag cgccattaat aggccgatag
     4141 ttttcagaaa agaaaaagtt tcattgtaca ggaaaacagc cagcagtgca ggtataacta
     4201 aagacatttt gctggcaata gttgttgaag caacaccatt cttttgtgaa gataaagcag
     4261 taaggtaaaa tacaccaata aacaaactcc cactcagaag gtaatagggg aaatagcttg
     4321 ctgcaaaccc tgaaattgaa ctgtaggttt gttttgaata taaggtataa caaataccgg
     4381 ccgtaaaata attaaatgtt agtccgtgaa tgttgctgac tttaaaacga tcgaatagtt
     4441 taagtatgac aaagaggagt gaggcaaaca gtacagccat gaaaacagta actgtcatac
     4501 gttgtaaaat atttttaatg tatcgtcttc aatgtttgtg atttcggaag gtgaaattaa
     4561 aaatacaggt gcttttattc cgctgattaa ttgtttagtt gcagtaaaca ctcttgcttc
     4621 atcccataaa ttgctttgaa taaagtattg cagtgttttt gctccgcctt caattattac
     4681 tgattggatg ttttgctgat ataatacttt cagaatttga gttgctggat tttcctgaaa
     4741 gtttattttt atatagtcaa tgttattcgc tctgttttct tttttctgat ttattaaaat
     4801 tgtctttaca gaaccatcga gcaagtgatg tgtcgcgggc acatctaagt ttttatcaat
     4861 gacaactcta attggagtac ttcctttcca aagtctgggg ttcaattgtg ggttgtctaa
     4921 gatagctgtt tttgttccta ccaatatagc ctgctcttct gttcgccatt tatgagataa
     4981 tgttttgcta taggtgttac ttatagcgta ttgttgctgc gcattttcag gtgcaataaa
     5041 gccatcagca ctttctgccc attttaaaat gacataaggt ctttgttttt catgaaaagt
     5101 aaaaaacctt ctgtttaaaa aacgacatgc atcattgagc attggtgtag cgacttcaac
     5161 attattttgc tgaagataac ttatgccttt gccatttact tttgctgtag tatcgagggt
     5221 gccaacaaca actttcttaa ttccttcctg aacaatacgc tcacagcaag gtgacgtttt
     5281 gccgtaatgc gaacatggct ctaatgaaac ataaagcgtt gaaagaggaa taaggttttt
     5341 attttctaca ctgtttattg cattgacttc tgcatgtgct tctcctgcgc gatgatgata
     5401 accttcacca ataattttat tttcaaaaac aagaacactg cccaccatag gattaggcat
     5461 agtttgtcct aaggccttct tggctagttc aagacaacga ttcattattg tttcgtcaaa
     5521 ggtcataacc atttgttgct gcaaaattaa aataaatgat gcgtttattt agctttgcaa
     5581 caatgacaag aaaggaaatt attaatgttt ttcaggcaga tgttgaatta atctatgaca
     5641 aaggtgaaag tagcgccatt accaagttag ttatggaata tattttcggc ttaaactaca
     5701 aatcaaatac tcatgaaaat gttaatgtgt cacaggaaac cttacttcgt cagtacctgg
     5761 agcgtttacg aaattacgag ccagtacaat atattttagg tcaggcatgg ttctgtaatc
     5821 atatctataa agttaactca agcgtactta ttccacgacc ggaaactgaa gagttaattc
     5881 tgcttatcaa gcaaaatctg aagtcatcac atattgggtc aataattgaa ataggtacag
     5941 gctccggttg catagctatt gagttgtcat tattatttgc acaggcaaca gtcattgcaa
     6001 cagatatttc agaaaatgca cttgaaatgg caagaaagaa tgctgagagt ttaagagctt
     6061 ctgtttcatt catcaacgaa tctctatttg tgccaagcag caaaacgatt caaactaaag
     6121 tagatttaat tgtttctaat ccgccttaca ttacgcaaca agaagctact caaatgcatc
     6181 ccaatgtatt gcagtgggag cctcatacag ctttatttgt aaaggaaaat ccgcttgaat
     6241 tttatattgc aatagcatct cttgcaacga atatattaaa tgacaaaggc tatgtttttt
     6301 tagagcttaa tcctgattat gcatttcagg tagcacagtg ttttacagag aaagggttta
     6361 cccaagttga aatagtaaaa gatatttcag gtaaggaacg ttttttaaaa gtacagtgat
     6421 taagtaaaaa ataaaatcag acttccgcac tgactttatt gtgccattcc atttcttttt
     6481 tcaaaaaagt tcctgtaatg ctgttggcgt ttttactgat ttgtaatggt gtgccctctg
     6541 caacaatatt tccacctctc attcctcctt caggaccaag atcaataata taatcagcag
     6601 ctttaataat atccatgtta tgttcaataa ctacaacggt gttgcctttg tcaaccaatt
     6661 gctgtaaaac caaaagtaaa attcttacat cttcaaaatg caggcctgtt gttggctcat
     6721 caagaatata aaaagtattg cctgtatctt tttttgaaag ttccgatgct aacttcacgc
     6781 gttgtgcttc accacctgat agggtagttg atgattgccc taatgttatg taacctaaac
     6841 caacgtcttg caacgtttta attttatgca agatagcggg aatgttgctg aaaaaatcaa
     6901 cagcttcatt gatactcaga ttcaatacat cgctgataga tttaccacga taacgaactt
     6961 caagtatttc gcgtttgtag cgtttgccgt tgcaagtctc acaatgtacg tgcacatcag
     7021 gcaaaaagtt catctgtata attttcaagc cagcacccag acaagtatca caccggccac
     7081 cactaacgtt gaatgaaaaa actcccgggg cataacctct tatttttgct tcgggcaatt
     7141 gtgaaaacaa atttcttatg tcagtaaaaa ctcctgtgta tgtagcagga ttactacgtg
     7201 gtgtacggcc aattggcgat tggtcaatct caataacttt atcaatatga ctcaatccat
     7261 ttattttttc ataaggcaat ggtttttgcg tgctgttata aaaatgtttg ttcaatgcag
     7321 gataaatagt ttcattaacc aaggttgact tgccgcttcc tgaaacacct gtaacgcaaa
     7381 ttaatgttcc taaaggaaaa agtgcagtca cattttttag attgtttcct ttagcacctg
     7441 caatggttaa tttttttcca ttgccctttc tgttttttga agaaggcatc ttaatgccac
     7501 cactcatata atttgctgtg agtgattgct gatactttag ctcttcaggc ttacctattg
     7561 ctacaatgtc accaccgtta atacctgcac cggggccaag gtcaacaaca taatcggcag
     7621 cttcaatagt ttctctgtca tgctcaacaa cgatgacggt attgccgata tcgcgaaggt
     7681 ctttcaatgc ttttatcagt cgttcattat cgcgctgatg cagtcctata gaaggttcgt
     7741 caagaatata taacacacct actaattgcg aaccaatttg tgtagctaaa cgaatacgct
     7801 gcgattcacc accggacaaa cttcttgtgc tacggtttaa ggtaatgtaa tcaagtccta
     7861 catcaagcag aaaacctaaa cgctttacaa tttctttaag tacttctgta gctatgatat
     7921 tttgtttttc actcagtgaa gcaggtagct gttgaaacca ttgctgcaac tcaccaaggt
     7981 ccatcaaaga aagttcagat atatttttat tgttgagttt aaatgcaagt gattcttttt
     8041 tcaatcttgt gccttcacat tctgggcaaa taatagtttg cataaaattc aacgcccact
     8101 ttttgatgga gtaagatgca ttttcttcca tctgattgac gataaagttt acgatacctt
     8161 caaaagtaat tccatgactg ctgcccggag ttgcattgag tgaaacatca tctgtgccgt
     8221 acaatattat gttcagtgct tcatgcggaa tatcatgaat tggtgtgttc aggttgaagc
     8281 caaaccttct gccaatgtat tccaactcac caaatatcca gttgttttta tattcaccta
     8341 aaggcaaaat tccacctttg cggattgatt ttttattgtc gggaatgatg gaagctaaat
     8401 ctgcttctgg tattgtgcca agtccgttgc aacgtggaca ggcaccgtaa ggagagttaa
     8461 aagaaaatgt gtttggtgca ggttcatcat aagagattcc tgaagtagga cacatcagat
     8521 tacgactgaa aaagcgaact cttttttgat catcagcaat aatcatcaat gtattcttgc
     8581 caaaacgcat ggcagttttt acggtgtcat aaagtctttt tgatgccgtt tcgttaattt
     8641 caattttgtc cactacaatt tcaatgtcat gagttttgta gcggtcggtt tgcatacctt
     8701 tttttatctc agtaatcaca ccatcaattc gcacacggat aaaacctgat actcttattt
     8761 gttcaaacag ttcgcggtaa tgtcctttac gtgctttgac aacaggagca agcacaacaa
     8821 tatttttctt gtcaaaatca cgaagaatta tttctacaat ctgatcatca ctcagtctca
     8881 ccattttttc acccgtcatg taggagaaag cttcaccggc acgtgcaaaa agcaaacgca
     8941 tgaaatcata aatttcagta accgttccta ctgttgagcg cggattctga ttggtagttt
     9001 tttgttcaat gctgataaca ggagagaggc cgtttatttt atctacatca gggcgttcca
     9061 ggctgccaag aaattgtctt gcataggcac tgaaactttc catatagcgt ctttgtcctt
     9121 cagcgtaaat agtgtcgaat gccaatgacg acttaccact gccgctgagt cctgtaaaga
     9181 caatcagttt ttcgcgcggt aaaacgaggt ctatattttt gagattatgt tcccttgctc
     9241 cgtaaatttc aatgctctcg tgctctgttt gcatatagta ataacctgca aaattaactt
     9301 attgcagcct cttatttgga tttgttaata aaaatgaagg cgctgttttt cttgatgcta
     9361 aatcgtcagc ataaattcat tgtaagaaaa tctgacagtt tagaaaaact tgttgagtga
     9421 tgaaccttgc ttgaaaaaat aaaataacaa caacggcagt ccgatattaa gatttatcag
     9481 atacagcaac tcactgttat gcgtaagctt ccagaacctt gcaatatatt gtgtaggaaa
     9541 ccattcttca acatgaaaaa ccggaacaaa accttcctgc tgtgtaaaac caacacgctc
     9601 tacatggccg gacaagacaa agtcgtatga aaacatgagt gcactctttg acttcttaaa
     9661 aggtgaactt cctacgagaa gagcaacatc ttttcgtaat tcgggataca ttttttttaa
     9721 gctatcaggg ataattaact cacctttttt aatgataata tcggcacatt ccggttcgca
     9781 aaattgctgt gttgctaaaa tagtattagg ctcgctatct tggcgatatg ctttaggctt
     9841 tttcaatggc agcaaccaat atccggtaaa tactacccat attaaaacca ataatttcaa
     9901 accccaaatc ctcatcactg caaaaatact aaaactgata ccttacctgt aactttattt
     9961 cacttctttt attattattt atttcatcaa gccctgagcc aaatgttttt tgattggtgt
    10021 aaacagtctg tgcaaaacgg aaccaaacat ctacaccttt ggtaacttta tagcgaagtg
    10081 ttacataata ccgaaaccca cggttataga atgatggaat agaataagaa tagagaatgt
    10141 cgttttcaaa agcataaatc ctcgaatcgt agctgtctgt atcaaataat ccatagcgca
    10201 cagagcctga aataggcgag ctaagcggtt taaataaaat atcctgataa atcataaatc
    10261 ctttttcatt gggcttttct tcataattaa ttctgaccca ttcaatacga tttctgatag
    10321 caacagcttt agaaattttg taagtaaggt tgaaacgata gttatcctgc cttttaaaca
    10381 caagataatc tgcaactgct gctgcactgc tgttttcttc tttttgtgtg cgcctgtttc
    10441 ggaaataaat ttcaaattgc cttgacggat tccatgacac ctgccataaa tattcataac
    10501 ctttagaagg tgcatcggca ccaaaacgga gccatggaaa tgtaaaacga tctaaatacc
    10561 cattaaaacc ccagtagcga tttggtctga ttacaatacc tgcataagtt cctttttcat
    10621 tccttgtagt acttgattca ccaaatccgc ttgcatagag aaaattaaag tcacgaggat
    10681 agtttctgtt cactacagaa acactaacgg tggttcctaa actgatgatg gcaccattaa
    10741 cataagcaat tgcaccatta tcacttcttg aaaattctcc gaagaaattg atgttgcgat
    10801 acaaccaact gtagtcaatg cccactttgg taaattgttt tccactgaag tcaaaacgat
    10861 tgtaaagttg aaacgagcgt tgcaaaggtc tgtcgtaacc tgcatgaact gctgttaatc
    10921 ctgctgtgaa attacgtgta ttgtaacctg cattgacacc atacatttgt tcgcgaatgg
    10981 tatttttgtt ttcaagttcg cttttggtgc gatgatagcc tgattcgata aacgaagtaa
    11041 aaatatcttg ttgctcaagc gtggtatcta aaatgctatt caggtttgca tctaattttc
    11101 tgttactata aaaagcatcg gcattccaat ttcgtttacc aacagaaact gctacacctc
    11161 ttttaaaact ggattcgttt agagaagtat aaggcctaat acctgtagaa aatttacgta
    11221 cagcagcaac atcgggcgac ttaccgaatg acaaacctgt acataatgtt aatccttgcc
    11281 cataaaataa atcataatca ccaataacag cacgttttat aattccgaaa tcacgaatag
    11341 ctaaatgtgc agagtaataa tcaaaaccat ttctttgtga gcctttaaaa aattcttctc
    11401 cctgatcttt ttctgctgtt aaattaaaac tgaatctttg tttgtagcga tactgatatt
    11461 gtgtataaag tttccatggg ctgccgggat aggcaggatg aactttgtct gttgctgtgc
    11521 tgtcgcgaaa gcctttttgt ttttcgagat aacgttggcc acgaacaata agtctgctgt
    11581 tgccatcagt aaatattttt tttaaggttg cttttatgtc ttctgtattt ccaactgtca
    11641 cataaggtaa aatttcttca atgtcttcta acgaaagaaa gtctattgcc tgaagttcgt
    11701 aaagcgaaat aaattttcct gaaatttcac ggtgttcaat aatagcatct gcctgcactt
    11761 cgctaaacaa tcccgtctgc aacaaatctt cacgtgtagc tttattcaga tttattgggt
    11821 tggcagctaa ttgctgaagt tgctctgtca gttcagtcag gtcagcagac tcatctgctg
    11881 tattggcaat gttttcaact ttctcttcaa tcttactgtc atcttgtggt ggcaccacct
    11941 gtgcaagtgt aatgttgcag aataatactg aaataaaaaa cagttgaatt gttttcataa
    12001 ttaaaaagca taacgcaaag ccaaccccgg actgtaacct aaaggttcaa aataacctga
    12061 agtaaagtca gcataaaatt gtgaaaattt taatccgaaa ccaaatgtaa gctgaactgg
    12121 ttctgtactc acgccaccac gaacatacaa tgccttaaca ggatgatatt ccagtgcaat
    12181 tttataagtt tcttttctgt caattgattt aaaaatttct gctgctgcca gaactttttc
    12241 ggaaaaggaa tagctgatac ctgccttgat gattacagga attcgctcat catcataatc
    12301 ggtaagtttt acatgcagtg gattaaaaat gtgtacacct gcttttagtt tgttaaactc
    12361 tgcaagcaca ccaccttcaa tggtgaagga tgatttgttt ccataacctt cgtcaatgtg
    12421 cgtgttaaga taatcaacct gaacaccggc actgaaaaca ggacttagtt tttttgcaaa
    12481 agcaaggccg attttattct gattaaaaag tttgtatcca taacggttta tactcaatgc
    12541 aaacacacct ttgttagatg ctagtggcaa agcaaaagcc gcagataatg tgttgagttc
    12601 tttcatgcca aagcggtttt gaatagccaa gcctgcttca ggtttttcta acattgacag
    12661 acaagcctga ttgttgaatg cagcgaatac atcattaaaa gtaacagcag catcgctcat
    12721 tgcagaggcg cgtgcacctc ctgatgtaag ataggtttga gcagatacac tgctgaaaca
    12781 gaaaagaaga atggccagta ttgttggctt cataagcatg ttttgacgaa ctcaaacata
    12841 gggcaaataa aattataatg caactaaaaa tatttcaatg agtaaatttt tgtcacattt
    12901 gactttatta ctatttgtat taagtttatt cagttatcgg aaacaggaaa ggaaccacct
    12961 tcataaggga gtatgaaaaa tttgttgtaa tacatattgt ttttgtacat ataatggtgt
    13021 aattttgaaa atcagttatc ctttatgaag actattactt attgttttgc catcattgtt
    13081 ttttcctgta acatgacatt tgcacaaaat tgtgatttgt tatgtgatgg tgattttgaa
    13141 aatccaactg ttatttcatc accacacctt gtcaattata agacaattga atgttggaaa
    13201 actactgcaa gtgacagtat cttggagttt tgggattccg gatttaatgg tgtgccttca
    13261 ttttccggta atcaattttt agaaataaat gcaaatgagg ttgccagtat ttatcaggat
    13321 gtttatttaa cagcaggaga tgtaataaca ttgcattttg cacatcgtgc tcgtgtcggc
    13381 attgatacac ttggggtttc agtttctttt caaaataata cacctgtaat tttaggatat
    13441 tatgcagacg atgatttagt atggggctat tatacagaac aatatactgt acctgctaca
    13501 ggacaatata gaatcagttt tatatcagtc tctaattttt ataatgtacc aactttagga
    13561 aattttatag atgcagttga ggtttgttta tcacctgttg gatttgatga agttaattta
    13621 caagaaacat ttttaatttc ttcccaaaag agcacattga aaatagactt tacatctcaa
    13681 ttaaaaggag aaggcatttt aacagtattt gatttgcagg ggaaagaagt atttagaatt
    13741 tcagtagata attcaagaaa gcaaattgcc acagctccat tgaatagtgg aatttatact
    13801 gttcaattga ttcaggggag atcaataaca gctaaaaaag ttttgattaa ttaacgaggt
    13861 ttttctgcaa ctataattac agaacctgca atttctttag caataggtat tttttcaact
    13921 acctgaccaa taccactcac caatgaaatt aaaaatgatg gcactacagg atgtaaaaaa
    13981 tcataaggaa aaatattaat ctgattaaat cctttttgtt caagcaactt actaaagttc
    14041 catcggttga tagcagtttc atccggtgaa tcaccaagcc attttttaat gaatggaata
    14101 tttttctgaa caaaaatctg cggattaatc atgttaggct cagcaaaaac catacggccg
    14161 cccggtttta atactcttaa aatttcattc agcgatcgtt caaattctaa gtgatgtaat
    14221 actgaacttc cgaatactac atcaaatgta tttgcatcaa attgtagttt catggcatct
    14281 gccaacacaa actctactgc gggatatttc tcttttgcaa catttagtaa ttcaggtgaa
    14341 aggtcagttg caataatttt agcacctgta cgctgataaa atttgcctga aaaaagtcct
    14401 gttccacaac ctatttctaa tactgtgctg cttttatctg cgagtgaata tttggctatt
    14461 aaatctgcac gtctgtcagc tctaaccttt cccgcaggac tgtcccagtt ccatattgct
    14521 tctgcaccac cctcaagtag ttttttgccg tgttctaatt cgtttgaaat tctatcactc
    14581 atcaatattt tatttcatat aggtaagaag gctcactagt tccaacctgc ttcactaatt
    14641 ttaatttatc aggatatttt tgtccaacag gccccatcat tctatgcaat gtgttgatga
    14701 catagccgtc attctttttt ggatttcgcc ttaaacttgc aattaacaca tgcgttactt
    14761 tattttcttt taacaatgca agaacagagt cagggtcagt gcttggtact gtatatatcg
    14821 gataaaatga tttgcctttg gaatagataa atgacatagg tgcttttcgt gaaacaacat
    14881 aagcagattc agggagactg tcaccgcaat aacgacttaa ttttaaaaag tttgtccaat
    14941 cttcggtata tccgtaataa atatcaccac ccatattctt ttttaatatc ggtaaattgg
    15001 ttacagactt atcaacagtt ttcatcagac tactcattat tataatgata ctaacaataa
    15061 gaaaaattga actgcgtagt gcgggagtgt tttgaaaagc atagtatagt ccataaaaaa
    15121 taatcatcag aataaatggc acgtaaacta aaaccattct tggttgatcc catgaggttt
    15181 gtaaaacaaa gaaggtcaat cctgccatca acaatacata cagaaatgta aattgcatga
    15241 ttttgttttt attcttgaaa atgaaataca aaccaatgcc aacaaaaaca aatgtaatca
    15301 acactagacc tgcattggtt ttggtattgt ccggtgaaac aaatcccaaa atctgataaa
    15361 gccttttacc aatataaata cttgtattag aaaaaagtct gtcaacaaat ccgccaaaat
    15421 catctacccc ttttgatgca ttgtaagggt ctttctgaat taaaatctga tactgattac
    15481 tgaattgatt ttgtgaaccc cagataaatg atttaataac ttcaaaaatg ccacgcacta
    15541 caacaaaaga accaaaggca taagctaatt ttttaaaatc gcgattgagg acaaaatata
    15601 atgctactgc agcaattgca cctacagtta tattttttgt tagtgacaac aaagtaagaa
    15661 taattccaaa aaccaaatat ttagcaatgg gtatgttatc ttctttagag gcactaagaa
    15721 taggccgtat aacaataaat agcaatgctt gtaaaaagca aaagaaagcc tcggagtagg
    15781 tttggctggc aaagtataag aagtagctat taattccggt aaaaattaaa actgcaaaca
    15841 atatagtgta aggtattagt ttgcgaaatg cataatacat tgcagttaaa ttcaacaaac
    15901 taaataaaat agaaagtgat ttgagaatag gtaagttaat accccaaatg ccaaccggca
    15961 agcttaaaaa aatcgggtag agtggcgctt tagtactgta ataactacta aaatctttgg
    16021 taagcttaaa tgcaccttca atataatcag agtcgtcatt tccttcactc atctttatgt
    16081 cgaagagtaa aactgaaaaa atcaatgata caagcaataa tgaaaaaaat attatagttt
    16141 catttttctg taaaaaattc tcaagtttta ttaaagtagt ttctcttgat ggtttattga
    16201 cagttttggt gctacttttt ttagcaacaa cttctttgtt ttttttcatt tcaatgaaag
    16261 ttattacaca aacttctttg catcagcaag aaacttttca agtccgatgt ctgtcaatgg
    16321 atgtttcaat aaatcggtaa tagcatttaa tgggcatgtg gctacatcag cgcctaactt
    16381 tgcacagttg atgatgtgca tggcatggcg tatgctggca gcaagaatct gtgtctgaaa
    16441 accatagttg tcgtaaatct gacggatttc actaatcagt tgtaaaccat cggtactcac
    16501 atcatccaag cggccaataa atggcgaaac ataggtggca cctgccttgg ctgcaagcaa
    16561 tgcctgtccc gctgaaaaaa ctaaagtgca attggtcttt attccttttg aagtaaaata
    16621 gtgaatggct tttattccat cttttatcat cggtacttta acaattatct gctcgtgcaa
    16681 cttagctaat tcttcgcctt cggcaacaat gccgtcatag ttggtggcaa taacttctgc
    16741 actgatgtcg ccttttacaa tattacagat gtcaacataa tgtttcatca ctgcagcttt
    16801 accttttatt ccttccttag ccatcagtga tgggttggtt gttacaccat caagtacgcc
    16861 taagtcattt gcttctttta tttgtgccag attggctgtg tctataaaaa atttcatttt
    16921 gtattttgtt tgatataagt gttacttatc tattgcttta cacaacagac taaaaatttc
    16981 gtcaatactt ccaatgccgt ttatgccgta atattttttt tgcatacggt agtattctgc
    17041 tacaggtgca gtcttattgt tgtattcgtt aattcgcttt ttaattattt caggattctg
    17101 atcatcaggg cgaccggaat ctttgccacg tagcaatagt cgcttcgata attcagcttc
    17161 cgttacttct aatgcaagca tcattttaat tgaagtgttt ttttgcttta ataaattatc
    17221 gagtgctgct gcctgtgccg ttgtgcgagg gaagccatca aaaataaatc ctttggcagt
    17281 tgggttggca tcaagttttg attctatcat tcctataact acttcatcgg gaactaatac
    17341 accctggtcc atcagttttt ttgcttcaag acccaattta gtttgtccgg caatttcgtt
    17401 tcgcaacaaa tcgcctgttg ataaatgtat gaggccatat tgctgaataa gtttttctga
    17461 ctgtgttcct ttgcctgcac ccggaggacc aaataataca atgttgagca tgataaaaaa
    17521 taaattaaag agggtttggt gatgaatttc tcatttcgtt ttattcgata ttatgatttt
    17581 agaacataaa tatcctgaag atttcgtccg tagccatcat agtctaagcc atagcctaca
    17641 acaaatgcag gtggaatttc aaaccctgca tagtcaactg taactttttt ctttaatgcc
    17701 tgtggtttaa aaagtaatgt tgccaattta accgatgccg gttgatgttt tgctatttcc
    17761 tgcatcagat atgaaactgt ctcgcctgta tcaactatat cttcaacaat aataacatgc
    17821 ctgttgttta tattccctga aagaccaact acatttttca tctgcccggt agattctgtg
    17881 ccgctatatg atgatactct gataaaagaa atttcgcatg gagtctcaat gttacggaat
    17941 agatcggcca taaaaagaaa ggcaccgtta agcacaccaa taaatacagg gtttttgtct
    18001 ttgtattcag cattcaggtc gcgtgcaatt tccagaattc tggtttcgat ttgctttcgc
    18061 ggaatcatga ttgtgaatgt tttatcaagt atggtaactt cgctcattgc cacttttttg
    18121 ggtgcggcta aagtaaaaat aaattcccta tgactgacgg ggtttctatt cttagcttaa
    18181 tttgttttca ttgccacagg ttctttattt ttgtttaata aatagcgtta attatgaatg
    18241 cgttcaatcc caatatagtt ataggtgttt taggtggtgg ccagcttggc agaatgttat
    18301 tgcaaaaagc ggcagattac aatctttcta ttcatatact tgatcctgat gaggctgcac
    18361 cttgccgcta tatgtgtaaa acatttgagc atggcgattt tcgcgattat caggctgtca
    18421 tggcctttgg taatgattgt gatattatta ccattgaaat tgaacatgta aacatagaag
    18481 cattatacga actggaaaag ttgggaaaaa aggtgtatcc tcaaccacat attcttgaaa
    18541 tggttcagga taaaggactt cagaaagaat tttatcagaa gaataattta cctacagcgc
    18601 cattttattt agtagaaaaa ccggaacaga taaagccgga gtttccattt gttcaaaaac
    18661 tgcgcaaagg tggttatgat ggtaaaggtg ttttggttgt taaagatgca gatggcatga
    18721 gtcagttgtt tcaggcacca agtgtaattg agaataaagt agttattgaa gcagaaattg
    18781 ctgtaattac agcaagaaat aattcgggag aaataaaatc atttccagta gttgaaatgc
    18841 agtttaatgc agaagccaat ttggttgaat tacttttttc tccatcaaaa acttcagccg
    18901 tcatacaaca gaaagcagtt tcgattgcag aagatttaat cagtaaactg aacatggttg
    18961 ggctgcttgc tgtagaaatg tttgttacaa aagatggaca agtgctgatt aatgaaattg
    19021 caccacgacc acacaatagc ggacatcata ccatagaagc aaatcatact tcgcaattcg
    19081 agcaacattt aagagcaatt cttaatttac cacttggtaa ctcatcaact attttgcctg
    19141 cagcaatgat taacctgtta ggtgataaag attctaatgg tagggcgatt tattcaggtg
    19201 tgcaagatgc attaaatgca ggtgatgtgc acattcattt atatggtaag aaaatcacta
    19261 aaccttatcg taaaatggga catgtaactg taacggcagc tactgttgac gaggcaattt
    19321 taaatgccca aaaaattcaa tcgcagttaa aagtaatcag ttgaagtaaa gtgtacgtaa
    19381 gcctatgtcc ttgccggtta tttttttgta ctctttcaat aattgttgat gaatggcaga
    19441 gtctgcctct tgcccttcta tcagcagcat gtcgttactg agtttaatta cagcaagatt
    19501 attttttatg aggccatttt cttttaaggt cgtaaacata taatcaacaa actttttgtt
    19561 tgcagcattt tctttgttta ttcccattga gctttttgcc gccatagctt ccgctacttc
    19621 atcctgatat tttgaccagt cggatttctc tacctcttta ccatttaaga caattcgttc
    19681 aacatcatca ccgctaattg aaattttaaa tgcaccatta tcattattaa tattcacagt
    19741 ttgtgtttca acaatgtatg catcatttgc actggcagca tgtgtaactt tgttatttaa
    19801 tggggcagga ttggtgattt ctgttttaac tgaaggtgtt tctgttaagt tttcttcagt
    19861 tgcctgaggt tgttcaatag cagcatcagg ctgtgtttct gttgcctgta atgcaggttg
    19921 agttgtttgc tctgtatttt cctgagttgg ctgtgttttt tgttgacctt gattctgaac
    19981 taaaaaataa attcctcctc caacaacagc aattgttgca acagcaatga caggctttag
    20041 ccatttcatg taacccaaag tggtttcttt tccttcaact tctttgttaa attgttctaa
    20101 actttcatcc agcgagacta caggatctgc atttcttgct gcatcaaaca atttattcat
    20161 cttattattt tccatggtga aaaaagtaat ttacaattat acaatttctt cttctaatat
    20221 ttttttaagt gactcgcgtg cccgggcaat tctgcttttc acagcagaaa gactatctcc
    20281 ctgaatctgc tgtatttctg ctaacgatag ccctgagatt tcaaacaaca ctatggcttc
    20341 tttctgtttt agcggcaatt gatctaaggc attatacaat tcttttaact gaactttgct
    20401 atcggcaccg gcatgatcat cactcacttg ttcagcttgt tccgggtcaa aggctgtata
    20461 gatttgcttt tttttatcct gaagcttata aaccaacctg cttgaaacgg taaacaaata
    20521 actcaataat gcatctttat ggcgaatggt ttcaaacttt tcataagcta ccaacattgt
    20581 ctctgcaaca atatctttta catcctcccg gttataaacc atagactgca caaagcgaac
    20641 cagcttttca tgtaattgct gataggcatc tgtgaaggct ttctgttttc ccgaaatttt
    20701 ctcctgaccg ctcatccatt taatgtaatg taagtgcgtt ttttcttcaa aaagtcgcac
    20761 tcctgataaa ataattttaa tggtctaaaa ataagaataa ttttacggcc acaaattgtg
    20821 ctgtatgcag gctttatcat caacaaattt taaatttcaa ggacaaacgg ctttttataa
    20881 gggtaaggtc agagatgtct attctattga taacaaatgg cttgtgatgg tcgttacaga
    20941 ccgtgtttcg gcatttgatg tggttttgcc aaggccaatt ccatataaag gagcagtttt
    21001 aaatcagatt gcagcacatt ttatgcaggc aactgccgat ttagtgccca attggattgt
    21061 tgatacaccc gatgttcagg tgtctatagg tattccatgt cagcctttaa gagtagaaat
    21121 ggttatcaga ggatatcttt ccggtcatgc ctggcgcgaa tacaaacagg gaaaaagaat
    21181 gttatgtggc gttaaattac ccgatggttt gaatgaaagt gatgccttgc ctgagccgat
    21241 aattacgcct actactaaag ctgcgcatgg tcacgatcag gatataagca aggaagaaat
    21301 aattaaacag ggaattgtgg atgctgcaat ttatgaacag gcagaatcct atacaaaaca
    21361 gttgtttaga agagggtcgg agatggctgc acagcgtggg ttgattttag tagataccaa
    21421 atatgagttt ggtgttcatc aaggcaaggt ttatcttatt gatgaaattc acacgcccga
    21481 ttcgtcacga tatttttatg cagatggtta tgcagaaagg caaaaaagaa agcaacctca
    21541 aaagcaattg tcaaaagaat ttttgagaca atggcttatt gaaaataatt ttcagggatt
    21601 agcgggacaa acagttccag aaatgtctga tgaaattgta cagatggtat cggatcgata
    21661 tattgaattg tttgaaaata ttacagggaa aacttttgta aaaccgtcaa acgaaaatat
    21721 agaacaacgc atttacggta atatagtaaa atcattagag aaacttacat gaggtttaat
    21781 tgacttaatt tgaaagagta aaataaactg agcaatatga acacagatga atgaataaga
    21841 agactgatgc ttaaattaag aatatttaac attcaaattt catcaagaac gttttacctt
    21901 tgtttaaaat aataaattat gaaaaaacaa attgcatcgt tactttttat tgcagccgtt
    21961 gtaagtacag cttctgcaca gaaaattaaa atccttgacg gtagtttagc aggattaaaa
    22021 ggagaaacta aactgaatgt tgtctatgat tatagtaata tgggcgtagg ctcttttgca
    22081 aaggaagaag agtacgttgc taaaaaaacc agagagtata atgcaaaaga acccggacgt
    22141 ggagatagtt gggcaaaaag ctgggtggca gatcgtaaaa acagatttga accaagtttt
    22201 gtagagctat tcaataaaaa atctccaatg ttaataggtg attttcctga tgcaaaatat
    22261 acaatgaagg ttagcacaac ttttacagag cccggtttta atgtttatgt taccagaaaa
    22321 aatgcatcca ttaatcttga aattactgtg tatgaaacag ccaatcctga taaaccaatt
    22381 tgtaaaatgc aaggtttgag atttcccggt cgtactttcg gtggctatga ttatgatacc
    22441 ggcacacgta tatctgagtc ttatgctaaa gcaggaaaag atttagctgc atttattgga
    22501 aaaaatttga agtagtaaat ttttagactt ttaaaaaaga gaagcggttc ttttagaacc
    22561 gcttctcttt tttaacatca ttcactgttt cattgcaaaa actttttcac ttccttttca
    22621 atataaactg aagtacttgg aaaagcaaaa tcacttttat tttcagctac tattttcatt
    22681 accccaaaat tgacttcctg cctagtgttt tcaaagtcgc tcaactcttg tgttttcaca
    22741 aaataaatga cttcaatttc taatgcactt gccccaaagt tggtaaagaa tacatttgga
    22801 ttttcgttaa ctaaagggtg ttcattgagc atattcaata tttgctgaat aatattctga
    22861 atctgatcag ctgttgtaga atataccaag cctattacaa aacgatgccg gtaaaatgtt
    22921 ctttcggttt gattttctaa ttctgcatcc atcatttttt tattcggaac aattactaaa
    22981 gttttttcaa tagtgcgtac tttagtggaa cggaaaccaa cttcttcaat atggccacga
    23041 agactgtcag tctttaccaa atcaccaacc acaaaaggtt tgtccataaa aatcaaaaat
    23101 gaccccaata ggttttctaa tgtgtcttta gaagctaaag ctatggctaa tcctccaatt
    23161 cctaaaccgg caatgatgct ggcaacattg agtttgaaaa ctgcaccaag aataaaaaat
    23221 aaactaatga ttacagttat tgctttcatt gatgatgcaa aaaacggaac aaataaatcg
    23281 tccatttttg attcggtttt gttggcacgc tcgcgcatta ccaatccaat gtaatctatc
    23341 actctgagta ttgtccaggt gataaaaaat acagtgacaa tctgaaaaat taattttatt
    23401 gtcattctta caccaaactc actttctgaa gctagtttcc attcatcagg aaaatgcaca
    23461 tacctgcatg caatgtatat gatgattaga aaaagtgttg ctgaaaatgg tttctgtgtc
    23521 agctcaacaa acttatttac accaatggtt ttgaaatatt ttttcagaac attgaagagt
    23581 aaaaaagtaa gaattcttga aataagattc ttgaagacaa gtcctgccag aataagtcct
    23641 gcaaaccata ggtagtcttc tatggtgttt cctaatattc tttgatgcag aacatctata
    23701 agcattacaa cagtttttta agagctattt cgaaagcagc agaggcaata ccttttttat
    23761 ctttatgggt agcaatcact tcctgcaatg catttttaat ggtggtggct gcatcatcaa
    23821 aaatcagtct gtcatcaata acggcatttg cattatgcag ataagcaaac actcttgcca
    23881 ttccacagtt agcaataaaa tcgggaatta cacttacatg tttgtcacaa tacaatccaa
    23941 tggggccgaa gaaaatctct ttatcagcaa aaggtacatt ggcaccacag gaaattactt
    24001 caagtttttt gttgatgagc gattgcattt gctcctgagt aaccaatcga gatgcagcac
    24061 ccggaataaa tatttctgca tcaaggttcc acactttttc tgccatttgt tgaggagaaa
    24121 gcatgttatc tgccaccagc ttgtttccct gacggttgtt gaataatgtt tttatttctt
    24181 caaacgtata gccgttttcg tttatcagtc cgccatctct gtcaatgatt cctacaattt
    24241 ttacgcccat ttgtgcaaga taaaatcctg cagcggcagc aacattgccc catccctgaa
    24301 tggcagctct tttctcagaa atttttccac cccataactc atagtaatat tttacggccg
    24361 atgcaacacc ataaccggta atcatatcgg caactctgta tttatggctc aaatcaggcg
    24421 atagcgaagc gtctttcaat accaatgaaa cgccttgctg caaattgctg atacggtttt
    24481 taatatttgt ttcgccatcg ttaaagtgac caactgcagt accttcctgc ggatgaagta
    24541 ttccaagttt attacaaatt ggaataacat catgtatttc gtcaacattc aaatcgccac
    24601 cggttccgta atatgttttc agcaatggct ttatggcctt aaaccaacgt tcaagaacct
    24661 gattttttct ggggtcagca gggtcgaagt taataccgga tttagctcct cctattggtg
    24721 gaccggatac agtaaacttc acttccattg ttttggcaag cgattcaact tctcttttgt
    24781 cgcatccttt gcgcattctt gttccgccac cggcagagcc attgcgcaag ctatttatta
    24841 caagccaccc ttcagcgcct gtttcagcat cactccattc aaatacaatt tcaggacgct
    24901 tctcttcaaa tttctttagt aaatccagca ttatgtttta aaaatttgca cgaaaatatt
    24961 aaaattttcc gcttaaaatt ctaataaaat tcttctgtta ttgaaacctc accaacactt
    25021 ttgaagtaaa atagtttatt attctaattt ttattttcaa tgacatgaga aatcaaagat
    25081 ttgaacaaaa aaagtccata aaagaccatt gggagacgga cggccatcat gcaaaaaagc
    25141 aaaagatggc taaccatcgt gaagaaaaaa actggaagaa caaaatcctc aaagaagagg
    25201 atgatgattc tgatgaagat tttttccggt atgatgaaga tgaatagcct taaggcataa
    25261 gatcctaaac taaagtttgt gatttaagtc attaggtaaa cggcaccacc aacagaatct
    25321 ttttttgctc ctgttacaga ggcaagtgtg tttttattgt tgtttattct caaatagcct
    25381 aaaagagcaa atatcaatgc ttctttaaag tcaattattt taacatcagg aacaataatt
    25441 tttacagttg ttttttcctg aattttttga attagaaatt tgttcttggc tccaccgcct
    25501 gtgattaatg cagtagtatt tgggttattt cctgcaaggg ttaacgcttg gctgatttga
    25561 taggcaatgt gctgtgtgat ggtgtgcagc ttatcattca gtgtaccggt tgaagagttt
    25621 attatcggaa gaaaatattc attaaaccac tcagcaccta aagaacgtgg ataaggtttg
    25681 cgataaaatt ctaatgcatt aagtttatca aaaagtgttg tatttaattt ccctgcagca
    25741 gcaagttttc cttctgcatc atactttagt ccaccgctat tggcaaggta gtttaacgcc
    25801 atatttacag gacaaatgtc aaatgcaatc cggctgtttt tcaccttgca tgaaatgttt
    25861 gcaatacctc caaggttaag acaataatca tacatgccaa aaaacaattc atcacctata
    25921 ggtactaaag gcgcaccttg tccgcctgat gcaacatcta aacttctgaa atcacaaacg
    25981 gtggttattc cagcaattgc agcaatatca gcaccgtgac caatttgtgt agtaaaacca
    26041 tttgcaggtt gatgaaaaac tgtgtgacca tgtgatgcaa ttaagtcgca agaaagttga
    26101 tgtttttgta aaaaatttaa agaactttct cctaaaaaaa caccaaattt gtgatgtaac
    26161 tcaaaaagtt tttcgccaga aagtaaatta gcattagcta attcatctct tcgtttttgg
    26221 tcatattcaa tagtctctgc agcaa
//
LOCUS       DAOOOI010000023        25942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_342_length_25942_cov_50.784139, whole genome shotgun sequence.
ACCESSION   DAOOOI010000023 DAOOOI010000000
VERSION     DAOOOI010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 25942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25942
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_342_length_25942_cov_50.784139"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="PKX59_04800"
     CDS             complement(<1..1437)
                     /locus_tag="PKX59_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOZ90220.1"
                     /translation="MTKYFFTLIFSSAILFAQAQKTPSKIELVHANTLEGDDALGKDV
                     RRLLGDVIFKQENTFMYCDSAYLYATTNSMDAFGNVRIEQGDTISLTGDILKYNGNSR
                     QARLLNNIVFSDRKMMLVSQNLNYNLDTGIADYVDGGQIVDGTDTLTSRFGTYFSNQK
                     KLAFKKDVVLRHPGHRMETDTLVYGTVSKVAEFYGPCYIRSNDSSVIYCENGWYNTTT
                     DKAFFSKNAYVQNKNQILKGDSMFFDNKTSAGEAFKNVSITDTTEKVVVNADYGNYNG
                     QKGKSFMTGNLLMTRMFEKDSLFMHGDSLISLFDSTGKAISYAVYHNVRMYKKDLQAT
                     CDSLIYTAADSLLFLYHNPVLWNEANQLTADTISMLISSDGIQKLYLNQSAFITSKED
                     SIRFNQLRGKTMVGYFADNMLSKIDVFGNGQSIYFARNKKNELTGVNRADCSNMIIII
                     KDSKISKLKLIEKFFQLKSEHEKMISEKD"
     gene            1722..2057
                     /locus_tag="PKX59_04805"
     CDS             1722..2057
                     /locus_tag="PKX59_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90221.1"
                     /translation="MDQQFEKIKAIESKIQKLIELNKNLAEEKAMYEDENRQLTEQVK
                     SLTEKMRQHEQKIDMQGQQLTQLEETNKSLNLATNETTETKEENKFLKSRIDEYVQEI
                     DKCLALLDK"
     gene            2065..2376
                     /locus_tag="PKX59_04810"
     CDS             2065..2376
                     /locus_tag="PKX59_04810"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOZ90222.1"
                     /translation="METVSMLIHVGNRQLNLKVPAADQENILKAEKLLNDKIAALRKS
                     YAVNDNQDILAMCAFSIAGELISQQASGKKSENTRMEKLESLDALLNGFLAQPELFNK
                     Y"
     gene            2539..4098
                     /gene="rny"
                     /locus_tag="PKX59_04815"
     CDS             2539..4098
                     /gene="rny"
                     /locus_tag="PKX59_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOZ90223.1"
                     /translation="MDPIIIGVGIGAALIAAIITFLVTKKNNAVIVGAAQQQADTLLK
                     EAEAKGEVIKKDKLLEAKEKFFQLKSEHEKMISEKDKKISDSENRVKQKERELNQKLE
                     QNSRKHQEYEALQTNLKGQMQIVEARKQEVDKSLQKHVQALEKIAGISQDEAKTQLME
                     TLKAEARTQAQSYIKEIMDEAKLTANKEAKRIIIQSIQRVATEHAVENSVTVFNLEND
                     DVKGRVIGREGRNIRALEAATGIEIIVDDTPEAIILSGFDPVRRELARLALHQLVSDG
                     RIHPARIEEVVNKVRKQLEEEIIETGKRTCIDLGIHGLAPELIRMVGRMKYRSSYGQN
                     LLQHSREVANLCAIMASELGLNAKLAKRAGLLHDIGKVPDDEPELPHAILGMKLAEKN
                     KENAEVCNAIGAHHDEIEMTSLISPIIQVCDAISGARPGARREIVEQYIKRLKDLEAL
                     ALSYPGVEKSFAIQAGRELRVIVESEKVSDQQADTLSFDLAQRIQTEMTYPGQVKVTV
                     IREKRAVNYAK"
     gene            4118..5392
                     /locus_tag="PKX59_04820"
     CDS             4118..5392
                     /locus_tag="PKX59_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOZ90224.1"
                     /translation="MAATEHKEYYRNLITLVTGSAFAQLISLALSPVFSRIYTPHEFG
                     LLAVFMSILSALSVIVCLRYEFAIIPARTGKDAFHLLELCFYSTLLITALTLVGVTIF
                     NLFFSTNAVLSKWIWFLPFMVLTNGSYQAMLSWANRHKSYKVISRYRIINSVVTNTII
                     LGAGLAGFISMGLLAGFVTGGVVASIMLYFNIQKQSEPFTLGFDLTALKNVAKKYEAF
                     PKVNLWQSIADMLLINGVIYFLTAFFSPAVVGLYGLTMRVLQAPFNLIGYSMGQVFIQ
                     DAAHQYNEGKNIQPIIKKSIQRTAFIAIPIVLPLILAGPQLFAFVFGENWRQSGEYAQ
                     ILAPWFFLDFIRTPLGQIPIIMGKQKRQFTITIAGNILMVISLLLGGFYFNNPLLVVT
                     LFTALQSLYLIYVIYWIYSISGVTSEIRDDSR"
     gene            5426..6745
                     /locus_tag="PKX59_04825"
     CDS             5426..6745
                     /locus_tag="PKX59_04825"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOZ90225.1"
                     /translation="MQFKTMTVTPNSIPANNTYQLLQKILCFCLAITSFMAVPYSKSI
                     AFDWFIQLPGSYKISLFNLVEIISFSTAFFIIRLRVLAGIERRILIALLLVMATRVIS
                     LLFAKQYESIQYFSILRFAEVLLGLIILTNLLQSKANRNIFIAGLACGLLIECFLGLN
                     MVIQTGGHLRGIFFGIPSYQMMVLFILFLFLLTKSQNLIILIFAINILLTGIMATQTR
                     AALIQLAVGMIVVIIIAYKQGFFKRFAITLVVVTIVHSITLYSMQSVISAAKERFEDA
                     IKEGKIHLEEDEHSVGGGTIQYRLYLWDKSIGAFLSHPVTGIGSGSFPRQLDSLPQKF
                     GVQLERNDKNLPLSTHNTFLGVLAETGIVGITAYFFWLSCIIILIIKYYRSSSHRSFE
                     TAVAIMLGIFIFSDFWSQQSFLPNMTYLTAYLLASLRSENYNPLPSS"
     gene            6749..7828
                     /locus_tag="PKX59_04830"
     CDS             6749..7828
                     /locus_tag="PKX59_04830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOZ90226.1"
                     /translation="MKILLLANADSIHTKKWCIALAERGFEIAVFSMSPTDTLWINEK
                     NIKLFVNNRSKKGEHHLSKITYLAALPALSKVIRQFKPDIVHAHYATSYGLLGYLSKF
                     KPFYISVWGSDIIDFPRKSFFHKLLLQKILKSSDKLMCSSQYLKRQLNDLVSKEVKVI
                     PFGVDTKLFRPTTSDKRFSGITIGIIKSLEPVYNIPLVIEALNILKNKYKIDNCKLLI
                     VGGGSLESKLKQQVNNLGLGNDVIFTGKVEQNKVVEYHNAIDIFINVSVYESLGVSVL
                     EASACEKPVIATNVGGLCEVVEDNKTGFLIDEVTPEKLAEKILFLINHPEKRNEMGVN
                     GRIYVIKYFEWEQSINQMVNEYLNN"
     gene            7840..9081
                     /locus_tag="PKX59_04835"
     CDS             7840..9081
                     /locus_tag="PKX59_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOZ90227.1"
                     /translation="MKFTILTQYYPPETGAPQNRLSDLALQLKNAGHDVTILTAMPNY
                     PQNEIHANYKGRYFISESLNGISILRSWIYVGKSRSVIPRLFNYFSFVISSFIWGIIK
                     LKKQDVIICESPPLFLGISGLLLSVFKKSKLVFNISDLWPESAEKLGVITNKTLLKLA
                     ERLELFLYRKSFLITGQTQGIVKNIGDRTNLSNLHWLPNGISDDVVNLNLNKDDCRKK
                     LNLADDSFIFSYTGVIGHAQGLEIIIHAAEKFVSKNVTIVFCGDGPELESLKALADKS
                     GFNHFVFTGAVDKKVALQVVAASDAAIIPLKRLDLFKGAIPSKIFENCALGKPILLGV
                     DGEAKELFVNQAQAGIYFEPENINELSTAMEKLFENNILCTSLGQNGKQYVLSKFSRK
                     QIAINFLNVLEQKLKDSTSTQ"
     gene            complement(9446..9790)
                     /locus_tag="PKX59_04840"
     CDS             complement(9446..9790)
                     /locus_tag="PKX59_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOZ90228.1"
                     /translation="MSKKKNESSGIVYSTNPDFKYNNDNNTESETPAPAQQDLRVLRD
                     SKQRGGKTVTLITGFVGKEEDIEKLTKMLKTKCGTGGTFKDGEIIIQGDQREKVFDLL
                     LAAGYKVKKAGG"
     gene            complement(9792..10664)
                     /locus_tag="PKX59_04845"
     CDS             complement(9792..10664)
                     /locus_tag="PKX59_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOZ90229.1"
                     /translation="MNSSKRVMFILNPKAGHGLNEDIIVVAGKILSKNFGECIFKKTE
                     FVGHATTLAAEAVKDKIDLVVCVGGDGTVNETAQALKGSETSLAIVPTGSGNGLARHL
                     RIPMNYKRAIGLINDGKKFAIDTFLVNDRFAVNLAGVGFDALVAHRFAALKVRGLQSY
                     IKTVIRSISDFEEMKFTDVSGQQQKAWLMCIANSSQFGNNARIAPAAQVNDGMLDVVM
                     IKRPSVLKMIPFAVKMFNGKLETSNSYRCIRVKKYSVDFEKEQPMHIDGEPAGMASSV
                     EVQLIPQSLNIIIP"
     gene            complement(10657..12498)
                     /locus_tag="PKX59_04850"
     CDS             complement(10657..12498)
                     /locus_tag="PKX59_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOZ90230.1"
                     /translation="MSNRKIVLSLLAFLIVIYSLLRLSFIIINLSSFSLSSPAEFLMV
                     LFAGLRFDLSAIFITNVLIAVILLLPFKFHRKSVKIVITTLFLLGNLPFLLFNVVDVG
                     YYHFTQKRTTADFFGVATMGDDFFNMLPSLLRDYWGLLVTFLILSVGLYKAFQWFLNR
                     RTGKSKWWFAEIVVTIGLTVLLFRGGLQYKPISVLTAARYTSNQNVAAILNTTFTILK
                     TLGKDELKNYNFDKKIPSALTYFNPMHNYANSGVFRRMNVVLIIMESFGKEYTGYFNQ
                     GKGYTPFLDSLMQHSLTCTDAYANGKKSIEGIPGIVSGIPALMNTPYISGQYNSNNLS
                     SLPFLLKKYGYTSSFYHGGNNGTMGFENFTKMAGYDHYVGRNEYGDKDYDGTWGVYDE
                     PFFQFFCHELNNQKQPFVATIFSLSSHHPYAIPPQYNSRFKEGEQPIHRAVRYADYAL
                     QQYFECAKKQPWYHNTLFVITADHTGPSIVSHYQTRTGIYEVPIVYFAPDDSLLQGQY
                     QNVTQHTDIVPGVLNYLHYPDKFKFFGNSIFDTTVSSCFAVSFLDGTYQLITNNSVTT
                     TDFDTEFHFVLRDAKTVNDSIKASNTLKVFVSLYNRSMIHNKLTEDE"
     gene            12666..13085
                     /locus_tag="PKX59_04855"
     CDS             12666..13085
                     /locus_tag="PKX59_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90231.1"
                     /translation="MKKSLLALSVAGMFLLSACGPSADEKAAEAKRVQDSIDAVNAAA
                     AEKAYQDSMAMVAEAQAAAEKAAQDSIAAAEEAAKNKKGGKPATKTVTKEEPKKVEPQ
                     KQSGSLKDKIGAETKNATDAGKKEGENAPKKLKDKLK"
     gene            13265..13797
                     /locus_tag="PKX59_04860"
                     /pseudo
     CDS             13265..13797
                     /locus_tag="PKX59_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655221.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            14075..15397
                     /locus_tag="PKX59_04865"
     CDS             14075..15397
                     /locus_tag="PKX59_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90232.1"
                     /translation="MKLRIIILLNVVFFNATAQIWQPFKNRFYNPAINDVIEFNSQIV
                     IGGSFLADSLYLNNIARWDGSDWQPFAQGMMGGINTMTIFNNQLYAGGGYLTIPNLTN
                     PGKIVRWDGVAWNKLNNGVTSGAEIYTMCEFGGSLYIGGRFTEVDGIPANNLARWDGT
                     SWYSVANISGTFPRVSAMVVYNNELYVGGYFEFVNGISMVNIAKYNGTIWSDVDMGVN
                     SNVIAMVVDSVNNRLYAAGNFTGCIGSGMLFPTNVAYWDGMTWHGVGTNPSINPRALC
                     FYKNSLYAGFSFEAIKANNDTLKYIAKWDGNEWQSVNKGVNDNVIELKVLNGMFIVAG
                     SFTQAGDSSVNYVAAYTDTTTSVHELNTVKQEITITPNPTKNSISVTSPVAVSAYSIT
                     SPTGQIIKKQNQKPLKEFSVDTSTLPNGLYIFTASMGKQLVSIKFAKE"
     gene            15584..16912
                     /locus_tag="PKX59_04870"
     CDS             15584..16912
                     /locus_tag="PKX59_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90233.1"
                     /translation="MKKLFCFLLLYQIGYGQVWKPLGHRFLYPGVFDFRNFNNQLLVA
                     GDYTADSSTLNNIARWDGSDWQPLDSGMLGGVYAMEEFNGELHIGGYFYNINNILNTR
                     KIARWDGVTFHPLAKGITSGGQVEVMLSYNGDLYVGGSFQTVDSLPIHTIAKWDGSAW
                     SPVCNITGTFAEVESMAIYNGDLYIGGYFTGINGQPMRNIARFDGISWSAVGDGLNSD
                     VYALVVDSATNRLYAAGNFTSTGTGAIIFPSNVAYWDGAVWTPVGSGPSLSPTSLTIY
                     RNQIYVGGYFSWINNIINSSGDTLNFICRWDGNDWQPLGKGLNCSVQALSVFNNDLIV
                     GGCFTMAGDSVVNLVAAYTDTTTSVQELNTVKQEITITPNPTKNSIAVTSPVAVTAYR
                     ITSPTGQIIKKQNQKPLKEFSVDTSALPNGLYIFTATMGKQLVSIKFTKE"
     gene            17032..17670
                     /locus_tag="PKX59_04875"
     CDS             17032..17670
                     /locus_tag="PKX59_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOZ90234.1"
                     /translation="MAEKLIILEPDKYYHIYNHAVGKENFFESDSDFNVFLEKFIKYA
                     SPICDVLTYCLMPNHLHFLVRIKNELRVMTIVKKPFLKNYSHNIVSISKNDILTDIIS
                     KQFSHLFNSYAKYYNYTHHRKGTLFSRAFRRKLIDSDDYLKQLICYIHQNPVNAGLCR
                     KPDEWKYSSYNALVNLKDTLVCRNEVIALFDDVDNFKFCNQVKVDIKLDFQE"
     gene            17749..18324
                     /locus_tag="PKX59_04880"
     CDS             17749..18324
                     /locus_tag="PKX59_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90235.1"
                     /translation="MRIKLLILFVFISLLGFAQNLVINPSFEDTISCISWPGWPQMQC
                     SYWIWGNGGSPDYFNLNYCGGANVPNNQFGYQFAKTGAAYCGLALYLTPMVQPNGREY
                     LGGQLNDTLKQGHTYCVSFYVAKADSSWYYTSSIGMYLSQNSSVDYSTALNLPYTPQI
                     VNTNGIIYDTLNWTQISGTYVAGGGKIFYHW"
     gene            18287..18697
                     /locus_tag="PKX59_04885"
     CDS             18287..18697
                     /locus_tag="PKX59_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90236.1"
                     /translation="MLRGGEKYFIIGNFNNDANTTLDSNNQANLFYAGSYYYIDDVSV
                     VDCTVGINEIESYKNKIKLMPNPAKDELRIMSDESGIKKIEVCDVLNNVMLAESAEAK
                     EKSITINVSNFASGTYFIKVFSNKGIAVKKFVKE"
     gene            complement(18830..19255)
                     /locus_tag="PKX59_04890"
     CDS             complement(18830..19255)
                     /locus_tag="PKX59_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90237.1"
                     /translation="MKKLSLSKTLLTGAVVALMAAGFTACKKDSDPDRKKFLGSFSVT
                     TGTISCGVSGNGTIAAGTAVVITENSGGDEKVNVSVGGQLSVVATANGNTITLDNQTV
                     SGFTYTGTGTLSSNTLTLSINEYDATVPENCVYSMTANK"
     gene            19813..21030
                     /locus_tag="PKX59_04895"
     CDS             19813..21030
                     /locus_tag="PKX59_04895"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOZ90238.1"
                     /translation="MTPDNTMKLNSIEEAIADLKNGKAVIVVDDADRENEGDFITAAH
                     NATPEMINFMASHGRGLICATLTEQRCEELALSMMVATNTSSHETPFTVSVDLLGHGC
                     TTGISASDRSKTIKALIDPATKPSDLGRPGHIFPLKSKNGGVLRRAGHTEAGVDLARL
                     AGFEPAGVLVEIMNEDGTMARLPDLVKIAKKFDLKIISIEDLISYRLKTESLIDREVD
                     VKLPTAYGSFELFAYKQNTTGQEHLALVKGEWKKDEPILVRVHSSCLTGDIFGSCRCD
                     CGPQLHKAMQMIEKEGKGVIVYMNQEGRGIGLLNKLKAYKLQEAGKDTVEANQELGFK
                     PDERDYGVGAQILRDLGVSKLKLMTNNPTKRAGLIGYGLEIVEIIPLEIESNEHNRHY
                     LQTKRDKMGHTIK"
     gene            21064..22722
                     /gene="recN"
                     /locus_tag="PKX59_04900"
     CDS             21064..22722
                     /gene="recN"
                     /locus_tag="PKX59_04900"
                     /inference="COORDINATES: protein motif:HMM:NF008121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOZ90239.1"
                     /translation="MLTRLAIKNYVLIRELDINFSDGLSIITGETGAGKSILLGAMGL
                     VLGERADAKTLFDKKGKCIIEAEFSISHYNLNDFFSQHDIDFESNTIIRREINSEGKS
                     RAFVNDTPVNLSVLKELSSYLVDVHSQHQTLTLNTSAFQLNVLDITAGNQKQLNDYSN
                     QFNEYKKLLKQLVELKTLESKSIAESEFLQFQFDELESAGLKQGEFEKIESELQLLNN
                     TENIVLTLDSTSDILSDGETNVLSNITQAVTRLNGIARLHPSLEDILKRIQSCNIELK
                     DISSELTALKSQFSLNPSQLEILNDRLSVYNKLQQKHRVKNADDLIVLQQEIANRLQT
                     FQSLTDDIEKCDKQTQDLYNKLKQIAAELHSSRQKAAQSFKKDILKLLKEVSLNDAQF
                     EVDLTATEELTATGTTAVRFLFSANKGVTVMELAKVASGGELSRLMLCIKALIANKTS
                     MPTIIFDEIDTGISGEVAHKVGNLIEQLSESRQVLAITHLPQMAGKGKTHYFVFKHIE
                     KGIAQSRIKTLSAEERVVEIAKMLSGEEVTDASLQNARHLLIKS"
     gene            22725..23129
                     /locus_tag="PKX59_04905"
     CDS             22725..23129
                     /locus_tag="PKX59_04905"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOZ90240.1"
                     /translation="MPTSEVTYIGELRVKSVHVFSGNEIITDAPLDNQGKAQAFSPTD
                     LLATSLANCMLTIMGIAARTHQFSIDGTRAEVTKTMAADPRRVSAIEVKLFFPKNNFS
                     DKEKKMIENAAHTCPVAYSLHPDIKQDISFNF"
     gene            23133..23606
                     /locus_tag="PKX59_04910"
     CDS             23133..23606
                     /locus_tag="PKX59_04910"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOZ90241.1"
                     /translation="MSNHNHFMEEAVALSKKAITDNEGGPFGCVVVKDGKIIAKGNNK
                     VTSTNDPTAHAEVVAIREACKALNSFQLDGCEIYTSCEPCPMCLGAIYWARPDVVYFA
                     NTRQEAAAIGFDDEFIYDEINVHPTERKIKMVHLPNLQAAGVFNSWQNKSDKTEY"
     gene            23626..25068
                     /locus_tag="PKX59_04915"
     CDS             23626..25068
                     /locus_tag="PKX59_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOZ90242.1"
                     /translation="MKLPFKKLTTLVLGSFIGPFIMTFFIALFVLLMQFLWKYIDDLV
                     GKGLDWWTITKLLGYTSTTLVPLALPLAILLSSIMTFGNLAEHYEIVATKSAGISLQR
                     LMRPLVVVAIIISLGAFYFSNNILPYSNLKMGSLLYDVRQQKPALFIKEGVFYNGIDG
                     YSIKIGKKADDGKSIKNIMIYDHTHNMGNKKVVIADSGTMVMTNDKRYLILTLINGHS
                     YEEQQNRKKNYDTNPLLRTQFQSETIRFDLSSFQLNRTNEDLFKDNYQMMNVKQLNAM
                     IDTFQLQAAQRKTRFARDIKPYYQFLKDSTLFIHVSNAIAKPFLTDSIKLNNTKFETA
                     LSQCRVIKSYIESADDDLESRNRAYVKYQIEWHRKFTLSIACLLLFLIGAPLGAIIRK
                     GGLGLPIVISIAFFLCYHIISITGEKMAKEGIISVERGMWQSSLVLLPIGIFMVYKAT
                     HDSALFDMDSYKSFFKKLFRKKRLDDFLPK"
     gene            25263..25514
                     /locus_tag="PKX59_04920"
     CDS             25263..25514
                     /locus_tag="PKX59_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015191801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOZ90243.1"
                     /translation="MSKYEKLLFKLLSGKNDTNFAFSDLCKLLDELGFNKRIKGSHHI
                     YYKNNVDEIINIQPDKAKAKPYQVRQVRNLIIKYKLIDI"
     gene            25511..25720
                     /locus_tag="PKX59_04925"
     CDS             25511..25720
                     /locus_tag="PKX59_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOZ90244.1"
                     /translation="MSKYEIIIYWSKDDKSYIAEVPELAGCMADGKSYQQALSNVEVI
                     INEWLETAKQLNRTIPKPKGKLMYA"
BASE COUNT         8501 a         4687 c         4902 g         7852 t
ORIGIN      
        1 gtctttttcg gaaatcatct tttcatgctc tgactttagc tgaaagaatt tttctataag
       61 tttaagtttg cttattttgc tgtctttaat aattataatc atgttgctgc aatcggcacg
      121 gttgacaccc gtaagttcgt tctttttatt tcttgcaaaa taaatgctct gtccattgcc
      181 aaaaacatca attttactca acatgttgtc tgcaaaatat ccaaccatag tttttcctct
      241 aagctgatta aacctaatgc tgtcttcttt tgaagtgata aatgccgact gattaagata
      301 taatttttga ataccgtcag atgaaataag cattgaaata gtatcggcag ttagctgatt
      361 tgcttcattc cacaacaccg gattgtgata taaaaacaac aaactgtcgg cagcagtgta
      421 aatcaacgaa tcgcaggttg cctgcaaatc ctttttatac attcttacgt tgtgataaac
      481 agcatacgaa attgctttac ctgttgaatc aaacaatgag ataagactgt cgccatgcat
      541 aaacaaactg tctttttcaa acattcttgt catcagcagg tttcctgtca taaaagactt
      601 acctttttgt ccattatagt ttccatagtc agcattaaca acaacttttt ctgttgtatc
      661 tgtaatgctt acatttttaa aagcttctcc tgctgaggtc ttattgtcaa aaaacatgct
      721 gtcgcctttc agaatctgat ttttattctg cacataagca tttttactga agaaagcttt
      781 atcggtagtt gtgttgtacc atccgttttc gcaataaata acagaactgt cgtttgagcg
      841 gatgtagcaa gggccataaa actctgcaac cttactaaca gttccataaa ccaaggtatc
      901 ggtttccatt ctgtgtccgg gatgcctgag cacaacatct ttcttaaagg ctaacttctt
      961 ctgattacta aagtatgtgc caaagcgact ggttaaagta tctgtgccat caacaatctg
     1021 cccgccatca acataatctg caatgcctgt atcaagatta taattgaggt tttgcgaaac
     1081 cagcatcatt tttctgtcgc taaaaacaat attgttgagt aaacgagcct gcctgctgtt
     1141 gccgttatac ttcagtatgt cacccgtaag actgatggta tctccctgtt caatccgcac
     1201 gtttccaaaa gcatccatac tgtttgttgt agcatagaga taggcgctgt cgcagtacat
     1261 aaatgtattt tcctgtttaa aaataacatc gcccaaaagc cttcgcacat cttttcctaa
     1321 agcatcatca ccctctaatg tattggcatg taccaattct atttttgaag gtgttttttg
     1381 agcctgagca aaaagaatgg cagaagaaaa aataagtgta aaaaaatact tggtcatgaa
     1441 gttggcaaaa ataagaggtt tcaatcaatc tcagtggaag caatgtttaa tgcaacaact
     1501 tcgctttggc cacacaaaca actgttttag aaccaaataa tcaaagctgc tttaatagaa
     1561 ttttaacata gaatttctga tttaattctt gtatttaagg tcaagttgct tttctttcga
     1621 ctaattaaca aagtattgtt gctaattttt agttaggcgc ggttgtaata cagtcatgaa
     1681 taatattttt actgaatcaa aaaccaatta atcaaataat tatggatcag caattcgaaa
     1741 aaatcaaagc aattgaatca aaaattcaaa aacttattga actcaataaa aacctggccg
     1801 aagaaaaagc catgtatgaa gatgaaaacc gtcagttgac agagcaggtt aaaagcctta
     1861 cagaaaagat gcgtcagcat gagcaaaaga ttgatatgca ggggcaacag ttgactcagt
     1921 tggaggaaac aaataaatcg cttaatttag ccaccaacga aacaacggaa acgaaagaag
     1981 agaataaatt tttgaaatca agaattgacg aatacgtgca ggagattgac aagtgtcttg
     2041 cattattgga taagtaagaa caaaatggaa acagtatcca tgcttataca tgtcggcaac
     2101 agacaactga acctgaaggt tcctgctgcc gatcaagaga atatcttaaa agccgaaaag
     2161 cttttgaatg ataagattgc tgcattgcgc aagtcgtatg ctgtcaatga caatcaggat
     2221 attctggcaa tgtgtgcatt tagtattgcc ggtgaactta taagccaaca ggcttccggc
     2281 aagaaatcag aaaatactcg aatggaaaaa ttagagtcgc tggatgcatt attaaacggt
     2341 tttctggctc agccggagtt gtttaataaa tactaataaa ccataattat agctgccttt
     2401 ctgtgcagcc tgttcattct gctttttcta aaacgttaaa ctgaatttaa atctttagac
     2461 ttaagattgg gttcgtgttt tgcagaatga aagtttaatc acatcctgtt aaagggtata
     2521 aaattatttt attaaaaaat ggatccaata atcataggcg ttggaatagg tgcagcattg
     2581 atagcggcaa ttattacctt ccttgtaaca aaaaagaaca atgctgtaat agtaggtgca
     2641 gcacaacaac aggccgatac gctgttaaag gaagctgaag ccaaaggcga agtcattaaa
     2701 aaagacaaac tgcttgaagc aaaagaaaaa ttctttcagc taaagtcaga gcatgaaaag
     2761 atgatttccg aaaaagacaa gaaaatttct gactcagaaa accgtgtcaa acaaaaagaa
     2821 cgcgaactga atcagaaact tgaacaaaat tcgcgcaaac atcaggagta cgaagcattg
     2881 cagaccaact taaaaggtca gatgcagatt gtagaagccc gcaaacaaga ggttgacaaa
     2941 tcgcttcaaa aacatgttca ggcacttgag aaaattgcag gcatcagtca ggatgaagct
     3001 aaaacgcagt taatggaaac gcttaaagcc gaagcacgca ctcaggcaca atcctatatt
     3061 aaagagatta tggatgaagc taaactcact gcaaacaaag aagcaaaacg catcatcatt
     3121 caaagtattc agcgtgtggc tactgagcat gccgttgaaa attctgttac cgtttttaat
     3181 cttgagaacg atgacgttaa aggccgtgta attggtcgcg aaggacgaaa catcagagca
     3241 ctggaagctg ccaccggaat tgaaattatt gttgacgaca caccggaagc aattattcta
     3301 agcggctttg atcctgtaag acgcgagtta gcacgtcttg cactgcatca gttagtgagt
     3361 gacggacgta ttcaccccgc ccgtattgag gaggtggtga ataaagttcg taagcaactt
     3421 gaagaagaaa ttatagaaac cggtaaacgc acctgtattg acctaggtat tcacggtctt
     3481 gcaccggaac tgattcgtat ggtaggcaga atgaaatacc gctcttctta tggacagaat
     3541 ttgttacaac actcgcgcga ggtagccaac ctatgtgcta ttatggccag cgaactagga
     3601 ttaaatgcca agcttgctaa acgtgcagga ttattacacg atattggaaa agtacccgat
     3661 gatgagcctg aattaccaca tgctattctg ggaatgaaac ttgccgaaaa aaataaagaa
     3721 aatgccgaag tatgcaatgc catcggtgcc catcacgatg aaattgaaat gacaagcctc
     3781 atcagtccta tcattcaggt ttgtgatgcc atttcaggcg cacgcccggg tgcaagacgc
     3841 gaaatcgttg agcaatacat caagagattg aaagatttgg aagcacttgc cctctcctat
     3901 cccggtgtag aaaaatcttt tgccattcag gcaggacgcg aattgcgtgt tattgttgaa
     3961 agcgaaaaag taagcgacca gcaagctgat acattgagtt ttgatcttgc acaaagaatt
     4021 caaacagaga tgacctatcc cggacaagta aaagttacgg ttatccgtga gaaacgtgcg
     4081 gtaaactacg ccaagtaaca gcctcgaggc agttttcatg gcggcaacag aacataaaga
     4141 gtactatcgc aacctgatta ctttagttac cggtagcgca tttgcgcagt taattagcct
     4201 ggcattgtca ccggtatttt caagaatata cacacctcat gagtttggcc tgctagctgt
     4261 tttcatgagc attttaagtg cattgtctgt aattgtttgt ctgcgttatg agtttgccat
     4321 catacctgca cgcacaggaa aagatgcatt tcacctgctt gaactttgtt tttattcaac
     4381 attgctgatt acggcattaa cgcttgtagg tgtaaccata ttcaatctgt ttttttcaac
     4441 caatgctgtc ttgtcaaaat ggatttggtt tctgcctttt atggtgctga caaacggttc
     4501 ctatcaggca atgctcagtt gggcaaacag acataaaagc tacaaagtaa tatcacgcta
     4561 cagaataatt aattcagttg ttaccaatac cattattctg ggtgcagggc ttgcaggttt
     4621 tatttcaatg ggattgcttg caggctttgt tacaggcggt gttgttgcct ccatcatgct
     4681 atactttaat attcaaaagc agtctgaacc atttacgctt ggctttgatt tgactgcatt
     4741 aaaaaatgtt gcaaaaaaat acgaagcttt tcctaaagta aatttatggc aatctattgc
     4801 cgatatgctg ctaattaacg gagtcatcta ttttctgact gcatttttca gccctgctgt
     4861 tgtaggtctg tacggactta ccatgagggt tttacaagct ccatttaacc tgataggata
     4921 cagcatggga caggttttta tacaggatgc agcacatcag tataatgaag gaaaaaatat
     4981 tcagcccatt attaaaaaat ctattcaacg cactgctttc atagctatac caattgtgtt
     5041 accattaatt ttagcagggc cacaactttt tgcttttgtc tttggcgaaa attggcggca
     5101 gtcaggcgag tatgctcaaa tacttgctcc ctggtttttt cttgacttta tcagaacacc
     5161 attggggcag attcctatca tcatgggcaa gcaaaaacga caattcacaa taacaattgc
     5221 cggaaatatt ttgatggtaa tttctttgtt gttaggtggc ttttatttta ataatccatt
     5281 gcttgtggtc accttattta cagcacttca atcgctatat ctcatttatg tcatttattg
     5341 gatctatagc atttctggtg taacctcaga aatcagggat gattcaaggt aattctctac
     5401 ccctgctata accttatttt tatttttgca gttcaagaca atgactgtta ctcccaatag
     5461 cataccggcc aacaacacgt atcaactgct tcaaaagatt ttatgcttct gcctggcaat
     5521 tacatctttt atggcagtgc cttactctaa aagtattgca ttcgactggt ttattcaact
     5581 gcccggaagt tataagatta gtctttttaa cttagtagaa ataataagtt tctcaactgc
     5641 ttttttcata atcaggcttc gggttttagc aggtattgaa agaagaattc tgattgccct
     5701 cttactagta atggcaaccc gagtaatttc tctgctcttt gcaaaacaat atgaatccat
     5761 tcaatatttc agcattctga ggtttgccga agttttatta ggattaatta tcctgaccaa
     5821 cctgttgcaa tctaaagcca acagaaatat ttttattgct ggattggcat gtgggttatt
     5881 gattgaatgt tttcttggct taaacatggt cattcaaaca ggaggacatc taagaggcat
     5941 tttcttcgga ataccatcat atcagatgat ggtgcttttc attctgtttt tattcttact
     6001 tacaaaatca cagaatttaa ttattttaat atttgccatt aatattttac ttaccggaat
     6061 aatggctaca caaacacgtg cagcattaat ccaattagca gtaggcatga ttgtagttat
     6121 cataattgcc tataaacaag gtttctttaa aagatttgct ataactttag tagttgttac
     6181 tattgttcat agcattacac tttattccat gcaatcagtt atcagtgcag caaaggaacg
     6241 atttgaagat gctatcaaag aaggaaaaat tcacttagaa gaagacgaac attcagttgg
     6301 cggtggcacc attcaatata gattgtattt atgggataaa agtattggag cctttcttag
     6361 ccatcctgta accggtattg gaagcggcag cttccctcga cagttagatt cactacctca
     6421 aaaattcgga gtgcaattag aacgtaatga taaaaatcta ccactgagta cacataatac
     6481 atttttagga gtgttggcag aaacaggaat agttggcatt acagcatatt ttttctggct
     6541 tagttgtata ataattttga ttataaaata ttaccgttct tcaagccata ggagttttga
     6601 aactgcagta gcaatcatgt taggaatatt tattttttca gatttttggt cacaacagtc
     6661 ttttcttccc aacatgacat atctgaccgc ctatttactt gcttcattac gcagcgaaaa
     6721 ttataatcca ctaccatcct cataagctat gaagattttg ctgcttgcta atgcagactc
     6781 tatccacacc aaaaaatggt gcatagcact tgctgaaaga ggctttgaga ttgcagtctt
     6841 ttctatgagc ccgactgata ctctttggat aaacgaaaag aatataaaac tgtttgtaaa
     6901 taatcgctcc aaaaaaggtg aacatcattt atctaaaata acctatctcg cagcattacc
     6961 tgctttaagt aaagtaatca gacaatttaa acccgatata gtacatgcac attatgcaac
     7021 aagttatgga ttgcttggct atctttcaaa atttaaaccg ttctatattt cagtttgggg
     7081 aagtgacatt attgattttc cacgtaaaag tttttttcat aaactgctct tgcaaaaaat
     7141 ccttaaaagc tctgataagc taatgtgttc aagccaatac ctaaaacgtc aattaaacga
     7201 cctagtatct aaagaagtta aagttatacc ctttggagtt gacacaaaat tattcagacc
     7261 aactacttct gataaaagat tttcaggtat tacaattgga atcattaagt cgcttgaacc
     7321 ggtttacaat attcctcttg taattgaagc attaaatatc ctgaaaaaca aatataaaat
     7381 tgacaattgc aaattactga ttgttggtgg tggctcttta gaaagtaagc tcaaacaaca
     7441 agtaaacaat ttaggtttag gtaatgatgt catatttacc ggaaaggtag aacaaaataa
     7501 agttgtagaa tatcataatg ccattgatat ctttataaat gtctctgtgt atgaaagcct
     7561 gggcgtttct gttctcgaag cttcagcttg tgaaaagcct gttattgcaa caaacgtggg
     7621 tgggctttgc gaagttgttg aagataataa aaccggtttc ttaattgatg aggtaactcc
     7681 agaaaaactt gctgaaaaaa tattattcct tatcaaccat ccggaaaaga gaaacgagat
     7741 gggcgtgaat ggaagaatct atgttatcaa atattttgag tgggaacaaa gcattaacca
     7801 aatggtaaat gaatacctca ataattaact ttgccatcca tgaaattcac catccttact
     7861 caatattacc ctcctgaaac aggcgctccg caaaatcgcc tttcagacct tgcattgcag
     7921 ttaaaaaacg caggacatga tgtaactatt cttactgcaa tgcctaatta tccgcagaat
     7981 gaaattcatg ccaactataa aggtcgctat tttatttcag aaagtttaaa tgggatttca
     8041 attctcagaa gttggatata tgtcggcaaa agccgctctg ttattccgcg actgttcaat
     8101 tatttttcat ttgttatttc gtctttcatc tggggaataa ttaaacttaa aaaacaagat
     8161 gtcattattt gtgaaagtcc cccactcttt cttggaattt caggattgct gctttccgtt
     8221 ttcaagaaat caaaacttgt gtttaatatc tccgacctat ggcctgagtc ggcagagaaa
     8281 ttaggtgtca tcacaaacaa aacacttttg aaacttgctg agcgactcga actttttctt
     8341 tacagaaaat cttttctcat taccggtcaa acgcagggta tagttaaaaa tattggtgac
     8401 agaactaatc tttctaacct acattggttg cctaatggca tttctgatga tgtggttaat
     8461 ttaaatctca acaaagacga ttgccgtaag aaattgaatc tggctgatga tagttttatc
     8521 tttagctata ccggtgtgat agggcatgca caaggattgg aaatcatcat tcacgcagca
     8581 gaaaaatttg tttctaaaaa tgtgacaatt gttttttgtg gtgatggccc cgaattggaa
     8641 agtttgaaag cattagcaga taagtcaggt tttaatcatt ttgtttttac cggtgcagtt
     8701 gacaaaaaag ttgcacttca agtagtagcc gcttctgatg cagcaattat ccctctcaaa
     8761 cgtcttgatc tttttaaagg agctatccca tctaaaattt ttgaaaactg cgcacttggc
     8821 aaacctattt tattaggtgt tgatggcgaa gcaaaagaat tatttgtcaa tcaggcacag
     8881 gcaggaattt attttgaacc ggaaaatata aatgagctat ccactgccat ggaaaaactg
     8941 tttgaaaata atatactctg cacctcatta ggacaaaacg gaaaacaata tgtgttgagc
     9001 aaattttcga gaaaacaaat tgcaatcaat tttctgaatg tattggaaca aaaactcaaa
     9061 gattccactt caactcaata agattttatt tctctcttca acagaacata cacaacatta
     9121 caagcctcct tccataaaat aatttttctc tgaaacaata cttaattttg agtagaatcg
     9181 ttgtaaaaat cagactaaga aattaacaat tttcagaggt gaaatcacgg tatattccgt
     9241 ttaccaaata aaaagggcta ttcacccaaa agtattttgt ataattgatg aaactttatt
     9301 tctttgtttt cgttgaaatt aattcaacat ctataaggtc gagtggccga gagtataggc
     9361 aaaagtccgc aagacttttt acagtagttc aaatctactc tcgacctcaa aaaaagccgt
     9421 cagcctgctg acggcttttt ttatgctatc ctcctgcctt cttaaccttg tatcctgctg
     9481 caagcaataa atcaaaaact ttttctcttt gatcaccctg aataattatt tcaccatcct
     9541 taaaagttcc tcctgtgcca cacttagtct ttaacatctt tgtgagtttc tcaatatctt
     9601 cttctttccc cacaaatccc gttatcagcg ttaccgtttt accaccacgt tgtttgctgt
     9661 ctcgcagcac tcttaaatcc tgctgtgccg gtgccggtgt ttcagactct gtattgttat
     9721 cgttgttgta tttaaaatca ggattggtac tataaacaat tcctgagctt tcgtttttct
     9781 tcttcgacat tttatggaat tataatattt aaagattgtg gaattaattg cacttcaacc
     9841 gatgaagcca ttccggcagg ctcgccatca atatgcatgg gttgttcctt ttcaaaatca
     9901 acagaatatt tttttactct gatacatcta taactatttg atgtttccaa tttaccatta
     9961 aacattttta cagcaaatgg aatcatcttc aaaacagatg gacgctttat catcacaacg
    10021 tccaacatac catcgtttac ctgtgctgcc ggtgcaatgc gtgcattatt tccaaattgc
    10081 gaactgttgg caatacacat caaccatgct ttttgttgct gccctgatac atcagtaaat
    10141 ttcatctctt caaaatcaga aatactgcgt attacagttt taatatacga ttgtaaccct
    10201 ctaactttta acgctgcaaa acgatgtgca acaagtgcat caaaacctac tcctgcaagg
    10261 tttacggcaa acctgtcatt aactaaaaaa gtatcaattg caaacttctt tccatcatta
    10321 attaacccaa tagctctttt gtaattcatc ggtatgcgca aatgacgtgc aagtccattt
    10381 cccgaacctg ttggtactat agctaatgat gtttcactac ctttcaaagc ctgtgctgtt
    10441 tcattaactg taccatcgcc accaacacaa acaactaaat caattttatc ttttactgct
    10501 tctgccgcaa gtgtagttgc atgtcctaca aattcagtct tcttaaaaat acattcgcca
    10561 aaatttttac tgagaatttt acccgcaaca acaataatat cctcgtttag tccatgcccg
    10621 gctttaggat tcagaataaa cattacgcgc ttgctgctat tcatcctccg taagtttatt
    10681 gtgaatcata cttctgttat acaaactaac aaaaaccttt aatgtatttg aagctttaat
    10741 tgaatcgttc actgtttttg catcacgcag tacaaaatga aactccgtat caaagtctgt
    10801 agttgtaacg ctgttgtttg taatcaactg ataagtacca tcaagaaaac taacagcaaa
    10861 gcaactgctg actgttgtat caaaaatact gttgccgaaa aatttaaatt tatcaggata
    10921 atgcagataa tttaatactc cgggaactat atctgtatgc tgtgtaacgt tttgatattg
    10981 tccttgcaac aggctatcat caggagcaaa atacacaatt ggcacttcgt aaatgcctgt
    11041 tcgtgtttga taatgactaa ctattgaagg acctgtatgg tcggcagtaa ttacaaaaag
    11101 tgtgttgtga taccacggtt gttttttagc acattcaaaa tattgctgaa gtgcataatc
    11161 ggcataacgc acagcacgat gtataggctg ttcaccttct ttaaatcttg aattgtactg
    11221 aggtggtata gcataaggat gatgcgatga aagcgaaaaa atggtggcaa caaatggttg
    11281 cttctgatta tttagttcat gacaaaaaaa ctgaaagaaa ggctcatcat aaacgcccca
    11341 cgtaccatca taatctttgt cgccatattc atttcttcct acataatgat catatccggc
    11401 catcttggta aagttctcaa aacccattgt accattattt cctccatgat aaaacgaaga
    11461 agtataacca tattttttca gtaagaatgg caaactactc aggttattgc tgttgtactg
    11521 tccgctgatg taaggcgtat tcatcaatgc cggaattccc gaaacaatac ccggtatgcc
    11581 ttcaatagat tttttaccat tggcataagc atcagtacag gttaaactgt gctgcatcaa
    11641 agaatctaaa aatggtgtgt aacccttgcc ctgattaaaa tagcctgtat attccttccc
    11701 aaaactctcc atgatgatta atacaacatt cattcgtcta aatactcctg aatttgcata
    11761 gttatgcatg ggattaaaat atgtcaatgc agaaggaatt tttttgtcaa agttgtagtt
    11821 cttgagttca tcttttccaa gtgtttttag gatagtaaat gtggtgttga gaatggcagc
    11881 tacattctga ttgctcgtat aacgtgcagc agtaagcaca gaaattggtt tgtattgaag
    11941 tccgcctctg aaaagcagaa cggttaaacc tatagtcaca acaatttctg caaaccacca
    12001 tttgcttttt cctgttctac ggtttaaaaa ccattgaaat gccttgtaaa gtccaacaga
    12061 aagaatcaga aatgtgacta acaaccccca atagtcacgt aacaatgacg gcaacatatt
    12121 gaagaaatcg tcacccatgg tagctacacc aaaaaaatcg gctgtggtgc gtttttgagt
    12181 aaaatgataa taaccgacat ctactacgtt aaaaagcaga aatggcaggt tgccaagcaa
    12241 gaataaggtt gttattacaa ttttcacaga tttccgatga aatttgaatg gcaacagtaa
    12301 tatgacggca ataagtacgt ttgtaataaa aatagcgctg aggtcaaagc gaagaccggc
    12361 aaaaagcacc attaaaaatt ctgccgggct actgagactg aaagaactaa ggtttattat
    12421 aataaaagaa aggcgtaata acgaatatat gacaatgagg aatgccaata acgacaatac
    12481 aatttttctg ttcgacatgc agagggcaaa tataacttat aggctttgta acctatagaa
    12541 gcaggtggtt ttagtgtcaa taaccattaa ctttaagctg ttaatatcta atcttgaaaa
    12601 aatattgaaa acctgacaat taaagtatta attttgcgac aaatttcaat caattcaaaa
    12661 taaaaatgaa aaaatcacta ctagcgctat cagtagcagg tatgtttctg ctgagcgcct
    12721 gcggaccaag cgcagatgaa aaagccgctg aggcaaaaag agtacaagat tctattgatg
    12781 cagttaatgc tgctgctgct gagaaagcct atcaggattc tatggcaatg gttgcagaag
    12841 cacaagctgc tgccgaaaaa gctgcacaag attctattgc tgctgctgaa gaagctgcta
    12901 aaaacaaaaa aggtggaaaa ccggctacta aaacagtaac taaggaagag cctaaaaaag
    12961 ttgagccaca gaaacaaagc ggttctttga aagataagat tggtgctgaa acaaaaaatg
    13021 ctactgacgc aggaaagaaa gaaggcgaaa atgcacctaa aaagcttaaa gataaattga
    13081 aataattttt cttaagtaat attaaagagg ctgtcccaaa agggcagcct cttttgtttg
    13141 ggtatgtctt gtacaaagtc ttttattatc actaattatg ctcccgtagg tgcgagcgtc
    13201 ctttccgctc gtgccattta ttttgtttgc atcttgcaaa ctataagact tactttgccg
    13261 gtgcatgagc gaaaaataca aaatatttcc tgatgggcta tactttgtta cattaaccac
    13321 agttggctgg attgatgttt ttacaagagc catttactgc gatgagatag ttaagaattt
    13381 aaattactgc attgacaata aaaagctaaa agtgtttgct ttttgcatta tgccatcaca
    13441 cattcattta attgcaaaaa cggaagacga tgtgagttta agcaatgtgt tacgcaactt
    13501 taagagttac acatcaaagc aaattattaa aatgattgaa gaaagccaca cggaaagcag
    13561 gcgcgaatgg ttgctttata tgtttgagtt ttacggcaaa aaaatgcgca caatgttaaa
    13621 tatcagtttt ggcaacagaa taatcatgcc ttcgatttat tcagtaataa atttattgat
    13681 caaaaagtaa attacataca caacaatcct gtagaagcac gaatagttac agagccacat
    13741 ttttatgtat atagcagtgc taatcaattt accgatttaa agctggctga actataaagt
    13801 ccgatagcta tcggacggcc ggaggccgca aggataaaag gcacgagcgg aaatgacgct
    13861 cgcgcctaca acagaaaaca caacttgcat cggtcatacc tttcggaacc aattatttta
    13921 ttttaccttt gggaaaacta accggagggg tttacaattg ccgtatttac agtggtagcg
    13981 atgtgctgag ccaaaagctg gtagtcgtta agtaaattta tttaattttg tatgtcgcaa
    14041 tgggttgatc ccattgcggc attattctta caggatgaaa ctaaggatta ttattttact
    14101 caacgttgtt ttttttaatg cgacagctca gatatggcaa ccatttaaaa acaggtttta
    14161 taatcctgca attaatgatg tcatagaatt taatagtcaa atagtaattg gcggcagttt
    14221 tttagcagat tcattatatc tgaataatat tgctcggtgg gatggatcgg actggcagcc
    14281 atttgcacag ggaatgatgg ggggaataaa tacaatgacg atattcaaca atcagttata
    14341 tgccggagga ggttatttaa caatacctaa ccttactaat cccggcaaaa tagtacgttg
    14401 ggatggtgtg gcatggaata aattaaataa tggagtaact tccggtgccg aaatttatac
    14461 catgtgcgaa ttcggaggaa gtttatacat aggaggaaga ttcactgaag ttgatggcat
    14521 tccagctaat aatttagcac ggtgggatgg aacttcatgg tatagtgtcg ctaatataag
    14581 tggtactttc cctcgtgtat ctgctatggt agtatataat aatgaactgt atgtgggagg
    14641 atattttgaa tttgtaaatg gaatttcaat ggtaaacatt gcaaaatata atggcacaat
    14701 atggagtgat gtagacatgg gggtaaatag taatgtaatt gcaatggtag ttgacagtgt
    14761 aaataacagg ttatacgcag caggaaattt tactggatgc attggaagtg ggatgttatt
    14821 ccctaccaat gtggcatatt gggatggtat gacatggcat ggtgtgggta caaatccctc
    14881 tattaatcct cgcgctttat gcttttataa aaacagcttg tatgctggtt tttcatttga
    14941 agcaataaag gcaaataatg atacgcttaa atacatagcc aaatgggatg gtaatgaatg
    15001 gcaatcggta aacaaagggg taaatgataa tgtaattgag ttaaaagtac taaatggtat
    15061 gtttattgtc gcaggtagtt ttactcaggc aggagattca tcggttaact atgtggctgc
    15121 ctatacggat accaccacat cggtacatga gttaaatacc gtaaagcagg aaataacaat
    15181 tactcccaat ccaacaaaaa acagtataag tgtaacatca ccagttgctg tttcggcata
    15241 tagcattact tcgccaactg gtcagataat aaaaaagcaa aatcaaaaac cgctaaaaga
    15301 gtttagtgtt gacacttcta ccttgcctaa tggtttgtac atttttactg catcaatggg
    15361 caaacagttg gtgagcataa aatttgcaaa agagtaagtc aggcttgttg taactatttt
    15421 gtgtttcctc tcaaaaatct tgatgaaggc ttatatgttt gcaagatttt tggcaagatt
    15481 tttggcaaga atataagcct gactaaaaag ctggtagtaa tcaaataaac gtttaattta
    15541 gcatcccgtc ctgtaatatt acgggacggg atgttttatg attatgaaaa agctattctg
    15601 ttttttgttg ctttatcaaa ttggctacgg tcaggtatgg aagccattag gccaccgttt
    15661 tttatatcct ggtgttttcg atttccgtaa ttttaataat caattacttg tagccggtga
    15721 ttacactgcg gattcttcca ccctaaataa tattgcacga tgggatggat cggactggca
    15781 gccgttagat agtggtatgt tagggggagt ttatgcaatg gaagaattta acggagaatt
    15841 acatattggc ggatactttt ataatataaa taacatattg aacacccgaa aaattgccag
    15901 atgggatgga gttacatttc atccgttagc aaaaggaatt acatcagggg gtcaggtaga
    15961 ggtaatgcta agctacaatg gtgatttata cgttggagga tcatttcaaa cggttgacag
    16021 cttaccgatt cacaccatag ccaaatggga tggcagcgca tggagcccgg tatgtaatat
    16081 aacaggcaca tttgcagaag tagaatctat ggctatttac aatggggatt tgtacattgg
    16141 gggctatttt acaggcataa acgggcaacc catgcgcaat attgcacggt ttgacggtat
    16201 atcgtggtcg gcagtgggcg atggattgaa cagcgatgtg tatgcattgg ttgtagatag
    16261 cgcaaccaac aggttatatg ctgcgggaaa ttttacaagc acgggcacag gtgctattat
    16321 ttttccatct aatgttgctt actgggatgg ggcagtgtgg acaccggtgg gtagcggacc
    16381 ttcattatcc cctacctcat taacgatata tagaaatcaa atttatgttg ggggatattt
    16441 ttcatggata aacaacataa tcaattcctc cggagatact ttaaacttta tatgcagatg
    16501 ggatggaaat gactggcagc cattaggcaa aggacttaat tgtagtgttc aggcattaag
    16561 tgtttttaat aacgatttaa tagtgggtgg gtgttttact atggcaggtg acagtgttgt
    16621 aaatttagtg gctgcctata cggataccac cacatcggta caggagttaa ataccgtaaa
    16681 gcaggaaata acaattactc ccaatccgac aaaaaacagt attgccgtta catcgcctgt
    16741 tgcagttact gcatacagaa ttacttcgcc aacagggcaa ataataaaaa agcaaaatca
    16801 aaagccgcta aaggagttta gtgttgacac ttctgcattg cctaatggtt tgtacatttt
    16861 tactgcaaca atgggcaaac agttggtgag cataaaattt acaaaagagt aagtcatggc
    16921 ttgtgtttta acctaagggg caggtcgcgt agctcctgtc agtgtgaaaa ctaattcaat
    16981 caaattgcaa tctaagagca taaaatcttt aattttcggt tttgaaaaac aatggctgaa
    17041 aaattaatta ttctagaacc ggataagtat tatcacatct acaatcatgc agttggtaaa
    17101 gaaaatttct ttgagtcgga ctcagacttt aatgtatttc ttgaaaagtt tattaagtat
    17161 gctagtccta tttgtgatgt attaacatat tgccttatgc caaatcattt acattttttg
    17221 gtgaggataa aaaatgaatt aagggtaatg actattgtca agaaaccttt cttaaaaaac
    17281 tatagtcata atattgtttc catttcaaaa aatgatattt taactgatat tatcagtaaa
    17341 caattttcac atttatttaa ttcctacgca aaatactata actatacaca tcacagaaaa
    17401 ggcacattgt tttctcgggc atttcgtaga aaattgatag atagtgatga ttatttaaag
    17461 caattaattt gttatataca tcaaaatcct gtaaatgccg gtttatgtcg taaacctgat
    17521 gaatggaagt attcttctta taatgcattg gtgaatctga aagacacatt ggtttgcaga
    17581 aatgaagtta tcgctttgtt tgatgatgtg gataatttta aattttgtaa tcaagtgaaa
    17641 gttgatataa aattggattt tcaggaatga atatagtata ttttttatac ttttgaaatc
    17701 acactgacag gggctgctcg acttgtcagg tgcaatcaaa attttgaaat gagaataaaa
    17761 ttactgatat tatttgtttt tatttctttg ctaggctttg cacaaaactt ggttatcaat
    17821 ccaagttttg aagatacaat ttcttgtata agctggccgg gctggccaca aatgcaatgc
    17881 agttactgga tttggggaaa tgggggaagt cctgattatt ttaatctcaa ttactgcggg
    17941 ggggctaatg ttccgaataa tcaatttggt tatcaatttg ccaaaacggg agctgcatat
    18001 tgcggattag cattatatct aaccccaatg gttcaaccta atgggagaga atatttggga
    18061 gggcaattaa atgatacctt aaagcaggga catacctatt gtgtgagttt ttatgtggca
    18121 aaagcagata gcagttggta ttacacaagc agtataggaa tgtatttgtc gcaaaacagt
    18181 tcggttgatt acagcacggc tttaaattta ccatatactc ctcaaatagt aaacactaac
    18241 ggcattattt atgatacctt aaactggaca caaataagcg gcacttatgt tgcggggggg
    18301 ggaaaaatat tttatcattg gtaattttaa taatgatgcc aatactactc ttgattccaa
    18361 taatcaagca aatttatttt atgccggctc ttattattac atagatgatg taagcgtagt
    18421 agattgtacg gttgggataa atgaaataga gagctataag aataaaataa agctgatgcc
    18481 aaacccagcg aaagatgaat taagaattat gagtgatgaa tcaggaatta aaaagattga
    18541 agtttgcgat gtgcttaaca atgtaatgtt agctgaaagt gcagaggcaa aagaaaaaag
    18601 tattacgata aatgtgagta attttgcaag tggaacttat tttataaaag tatttagtaa
    18661 taaaggtata gcagttaaaa agtttgtaaa ggaataatgt actcattaat tcgtatatca
    18721 cctgcctgtg attaattgaa ctaaatcacc ctgtttttca aaaacacaat ctgtcttaaa
    18781 acaaaaaaag agggcatagc cctctttttc tatactttat gctaaacaat tatttgttag
    18841 cagtcatgct ataaacgcaa ttctcaggaa ctgtagcatc atactcattg atagacaatg
    18901 taagtgtgtt gctgcttaat gtacctgtac cggtgtaagt aaatccactt acagtttggt
    18961 tgtcaagggt aatagtgtta ccgtttgcag tagcaactac tgataattgg ccaccaacgc
    19021 taacgtttac tttttcatca ccaccggaat tttcagtgat aactacagca gtacctgctg
    19081 caattgttcc gtttccagaa acaccacaag aaattgttcc tgttgttaca gagaaagaac
    19141 ctaagaattt cttacgatca ggatcgctgt cttttttaca tgcggtaaat cctgctgcca
    19201 ttagggctac tacagcacct gtcaaaagtg tttttgataa gcttaatttt ttcattttta
    19261 aataaagttt ataatgttta tgcgggcaaa tatagcatga attatcttac caagccacta
    19321 ttttttaact gaaagttatt cacgtttaga tgtgaaaacc ataaaaccct ttaaaagcaa
    19381 aaaatcaccg ccacgttggc ggtgattttt aaaagtataa ccctaaaacc aaatccgaat
    19441 ttaaaatgca gatgtcgggc tgtcttacgg ggcagcccac acctgaaacc aacctaaaac
    19501 tattttttgc ttaggtgatt caacttttag ccttacaggg ctaaggatga atcattttaa
    19561 tattaaccta aaaccaaact atcttagtaa taatctttat caagaacgtt atgtgcaatt
    19621 taacgcaggc tttattcctt ttgttacact actataaacg tacattacgt atgtaagatt
    19681 atatacctat tacaatcttc acttcatctt aacatacaga aaaatgacaa aaaaccagcc
    19741 tattctactg taagaatttt atctaaacat tgaaaagctt gttgtaaatt tgcaaaaaca
    19801 gatttagaga caatgactcc tgacaataca atgaaattga atagtataga agaagctata
    19861 gccgatctta aaaatggcaa ggcggtgata gtagttgatg atgccgaccg cgaaaatgag
    19921 ggcgacttta ttactgccgc ccacaatgcc actcctgaaa tgataaattt tatggccagc
    19981 catggcagag gccttatatg tgctactctt acagaacaac gctgcgaaga gttggctttg
    20041 agcatgatgg tggcaacaaa tacttcctct cacgaaacac catttaccgt ttctgttgat
    20101 ttgcttggac atggatgcac cacaggaatt tcggcctccg accgctctaa aaccattaaa
    20161 gcattaattg acccggccac aaaaccttca gaccttggcc gccccggaca tattttccct
    20221 cttaaaagta aaaatggtgg cgttttaaga agagcaggac atacagaagc aggtgttgac
    20281 cttgcccgtc ttgccggatt cgagcctgca ggtgtgcttg tagaaattat gaatgaagat
    20341 ggaacaatgg cacgacttcc agatttggta aaaattgcaa aaaagtttga cctgaaaata
    20401 atttctattg aagaccttat ctcctaccgg ctgaaaacag aatcgctcat tgatcgggaa
    20461 gtggatgtta aactgcctac tgcctatggt agctttgagc ttttcgccta taaacaaaac
    20521 acaacaggac aagagcatct tgctttagta aaaggcgaat ggaaaaaaga cgagcctatt
    20581 ttagtgaggg ttcactcctc ctgccttaca ggtgatattt ttggttcttg ccgctgcgac
    20641 tgcgggccgc aactacataa agccatgcag atgatagaaa aagaaggaaa gggtgtaatt
    20701 gtttatatga atcaggaagg acgtggcatc ggattgctga ataaacttaa agcatataaa
    20761 ctgcaggaag caggaaaaga tactgttgaa gccaatcagg aactgggttt taaacctgat
    20821 gaaagagatt atggtgttgg cgcacaaatt ctacgcgatt taggtgtttc taaattaaaa
    20881 ctcatgacca acaaccctac aaagcgtgcc ggattgattg gctatggctt agagattgtt
    20941 gaaattattc ctttagaaat tgagtccaac gaacacaacc gacattatct gcaaacaaag
    21001 cgcgataaaa tgggacacac aattaaataa tcaacaggat attattttaa gaaacaacat
    21061 tgtatgttaa cccgtcttgc cataaaaaat tatgtgctta tcagagaatt ggatattaat
    21121 ttttctgatg ggttaagtat tatcacaggc gagacaggtg caggaaaatc tattctgctg
    21181 ggtgcaatgg ggcttgtttt aggagaacgt gctgatgcaa aaacactttt cgataaaaaa
    21241 ggtaaatgta ttattgaagc agaattttca atttctcatt acaacctgaa tgattttttt
    21301 tcgcagcatg atatagattt tgaatccaac accatcatca gaagagaaat taattcagaa
    21361 ggaaaatcgc gtgcatttgt taacgacact cccgtaaacc tttctgttct gaaagaactc
    21421 agtagctact tggttgatgt tcattcgcaa catcagacat tgacattaaa tacttcggca
    21481 tttcagttaa atgtattaga cattacagca ggcaatcaga agcagttaaa tgattacagc
    21541 aaccaattca atgaatataa aaaattattg aaacaacttg tagaactgaa aacattggag
    21601 agtaagagta ttgcagaatc tgagttttta cagtttcagt ttgacgaact tgaatcggca
    21661 ggattaaagc aaggtgagtt tgaaaaaatt gaatcagaac tgcaactact taacaatact
    21721 gaaaatattg tattaacact tgacagcaca agtgatattt tgtctgatgg cgagacaaat
    21781 gtgctttcaa acattacaca agctgtaacc cgtttaaatg gaattgcacg attacatcca
    21841 tcgcttgaag acattctgaa aagaattcag tcgtgcaaca ttgaactgaa agatatttca
    21901 tcggaattaa ctgcacttaa aagccagttt agtttaaacc catcacagct tgaaatactc
    21961 aatgacagac tttctgttta taacaagcta caacaaaaac acagagtaaa aaatgctgat
    22021 gacctgatag tcttgcagca ggaaattgcc aacagacttc aaacttttca atcgcttact
    22081 gacgatattg agaaatgcga taaacaaact caggatttat ataacaaact aaaacaaatt
    22141 gcggcagaac tccatagcag cagacagaaa gctgcacaaa gtttcaaaaa agatattttg
    22201 aaattactga aagaagtttc gttaaacgat gctcagtttg aagttgacct gacagccaca
    22261 gaagagttaa cagcaacggg cacaactgca gtgcgctttt tattttctgc aaacaaagga
    22321 gtaacggtaa tggaattggc gaaggttgct tccggtggtg aactttcacg actgatgtta
    22381 tgtattaaag cacttatagc caacaaaaca tcaatgccaa ccatcatctt tgatgagatt
    22441 gacacaggca tctccggtga ggtagcccat aaagttggta accttattga acaactttca
    22501 gaaagcagac aagtacttgc cataacacac ctaccgcaaa tggcgggcaa aggaaaaacg
    22561 cattactttg tttttaaaca tattgaaaaa ggaattgcac aatcaagaat aaaaaccctt
    22621 tctgctgaag aaagagttgt tgaaattgca aaaatgttaa gcggtgaaga ggttacagat
    22681 gcaagcctcc aaaatgcacg acatctactt ataaaatcat aattatgccc acatcagaag
    22741 ttacctatat aggcgaatta agagtaaaat cggttcatgt tttttcagga aatgaaatca
    22801 tcaccgatgc gcctctcgac aatcagggaa aagcacaggc cttctccccc accgaccttt
    22861 tagcaacatc actagccaac tgcatgctca ctattatggg catcgctgca agaacacatc
    22921 agttttcaat tgatggcaca cgagcagaag taacaaaaac tatggccgct gaccctagaa
    22981 gagtgagcgc tattgaagtg aagttgtttt ttccgaaaaa taatttttct gataaggaaa
    23041 agaaaatgat agaaaatgca gcccacacct gtcctgttgc atacagtctc catcccgata
    23101 tcaaacaaga tatcagtttt aatttttaat aaatgagcaa tcacaatcat tttatggaag
    23161 aggctgttgc cctttccaag aaagcaatca ctgacaatga aggcggccct tttggctgtg
    23221 ttgttgttaa agacggaaaa attattgcta aaggaaataa taaagttacc tctaccaacg
    23281 accctaccgc acatgcagaa gtagttgcca tccgcgaggc ttgtaaagca ctcaacagtt
    23341 ttcagttaga tggctgcgaa atttatactt cctgcgagcc ttgccctatg tgccttggag
    23401 ctatttattg ggcacgaccg gatgttgttt acttcgccaa taccagacag gaagctgctg
    23461 caataggttt tgatgatgaa tttatttatg atgaaataaa tgttcacccc accgagagaa
    23521 aaataaaaat ggtgcattta cccaacctgc aggctgccgg tgtttttaat tcatggcaaa
    23581 ataaatctga caaaaccgag tattgatatc agcaacagat aattgatgaa attaccattt
    23641 aagaaactca ccacacttgt attaggttct tttatcggtc cttttatcat gacatttttt
    23701 atagcactct ttgtgctgct gatgcaattc ttgtggaagt atattgatga tttagttggc
    23761 aaaggactcg actggtggac catcactaaa cttcttggct acacatcaac caccttagtt
    23821 cctttggcat tgccattagc tatattgttg tcatcaataa tgacttttgg caaccttgcg
    23881 gaacactatg agattgttgc cacaaaatcg gcaggtattt ctttacaaag gttaatgcgc
    23941 ccattagtag tggttgcaat aataatttct ttaggcgcct tttatttttc aaacaacatt
    24001 ttaccttaca gtaatcttaa aatgggctcg ctgctttatg atgtgcgtca acaaaagccg
    24061 gcattgttta ttaaagaagg tgttttttac aatggcattg atggctacag tattaaaata
    24121 ggtaaaaaag ctgatgatgg taaatcaatc aaaaacatca tgatttatga tcatacacac
    24181 aacatgggta ataaaaaagt tgtgattgcc gatagtggca ctatggtgat gacaaatgat
    24241 aagcgttatc ttattctaac tttaataaat gggcattcat acgaagaaca acaaaaccgt
    24301 aaaaaaaatt acgacaccaa tccactactc agaacgcaat tccaatccga aactattcgt
    24361 tttgacttat cgtcttttca gctaaacaga accaatgaag acctgtttaa agacaactat
    24421 cagatgatga atgtcaaaca gcttaatgcc atgattgata cttttcaatt acaggcagca
    24481 cagcgaaaaa cacgttttgc cagagacata aaaccatatt atcagttttt aaaggactca
    24541 acattgttta tccatgttag caatgccatt gcaaaacctt ttctaactga tagcatcaaa
    24601 ctaaacaaca ccaaatttga aacagctttg tcgcaatgtc gtgtcatcaa atcgtatata
    24661 gaaagtgctg atgatgattt agagagcaga aatcgtgcct atgtaaaata tcaaattgag
    24721 tggcatcgca aattcacttt atcaattgcc tgtttattgc tgttcttaat tggagcacct
    24781 ttaggtgcca tcatccgcaa aggcggtctt ggactaccta ttgtaatttc cattgcattc
    24841 ttcctctgct atcacattat ttccatcaca ggagaaaaga tggcaaaaga aggaatcatc
    24901 agtgtagaaa gaggcatgtg gcaatcgtca ttggtgttgc tgcctatcgg aatttttatg
    24961 gtttacaaag ccacacatga ctcagccttg tttgatatgg actcttataa gtcatttttc
    25021 aaaaaactgt tccggaaaaa acggcttgat gactttttac ctaagtaggt gcgagcgtgt
    25081 cgctcgtgtc atttaactat tttcgctttt agcgaaagag agattaattt tatggctgca
    25141 agccattaaa ataataggta acgaggcaca gcctcgcacc tactgagaac agaggctacg
    25201 ctgatttgtc tagtgaaatc aataattgta tatttgctct ttctattttg tcttgattgc
    25261 ctatgagtaa atatgaaaag ttgttattta aattgttgag tggaaaaaat gacactaatt
    25321 ttgcttttag cgatttatgt aaactgttgg atgaattagg atttaataaa agaattaaag
    25381 gcagccatca tatttattat aaaaataatg ttgatgaaat aataaatatt caacctgata
    25441 aagcgaaagc aaaaccttat caggttagac aagtaagaaa tctgattata aaatataaac
    25501 tgattgatat atgagtaaat atgaaatcat tatttattgg agtaaggatg ataaaagcta
    25561 cattgccgaa gtacctgaac tggcaggatg catggctgac ggaaaatcat atcaacaggc
    25621 attgtcaaat gtggaagtga ttattaacga gtggttggaa acagccaagc agttaaaccg
    25681 caccattcca aaacccaaag gaaaactgat gtatgcctga cctttcctgc cgcccatctt
    25741 tgtaagcgcg agcgtgtcgc tcgtgtcatt ttattatttt cgcttttagc gaaagagaga
    25801 ttaattttat ggctgcaagc cattaaaata ataggcaacg aggcacagcc tcgcacctac
    25861 agatattccc ctctggtgca agtcttcaga cttgcgccca atgtcattaa gttaaggaat
    25921 aagaaattgg agttcaaatt ac
//
LOCUS       DAOOOI010000024        25762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_344_length_25762_cov_26.088264, whole genome shotgun sequence.
ACCESSION   DAOOOI010000024 DAOOOI010000000
VERSION     DAOOOI010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 25762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25762
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_344_length_25762_cov_26.088264"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PKX59_04930"
     CDS             complement(<1..604)
                     /locus_tag="PKX59_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90245.1"
                     /translation="MEAVLSIGYIALFVFVIHKSAFFRIEGIPVWWLKAAFLVKIIFA
                     ILLGVIYSKYYTDRLTADTFKFFDDSKILYDLAFEHPLWFLQAVFGINDQASYLMPYY
                     DTMHNWFNKVVLFNDYRFQIRLNALMRFISLGYYHVHAVFYCFLSFVGLTALLKLFVK
                     ALPELRRVLFAGVFFIPSVLFWGSGLLKDSLILFATGMALY"
     gene            725..2956
                     /gene="purL"
                     /locus_tag="PKX59_04935"
     CDS             725..2956
                     /gene="purL"
                     /locus_tag="PKX59_04935"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOZ90246.1"
                     /translation="MESLKETTVETAKKLGLLPEEFDKICSLMGRKPNFNELSIYSVM
                     WSEHCSYKNSITWLKTLPREGPHMLAKAGEENAGLVDIGDNLACAFKIESHNHPSAIE
                     PYQGAATGVGGINRDIFTMGARPIAQLNSLRFGNLNNERTKMLMRGVVKGIGDYGNAF
                     GVPTVGGEVYFEDCYNTNILVNAMSVGIVEHGKTVKAISEGEGNSVYIVGSATGKDGI
                     HGATFASGDLHEDSHEDLPSVQVGDPFQEKLLLEATLEVIATGAVVGMQDMGAAGIIC
                     STSEMSAKGNHGMIIHLDKVPTRQKNMEPWEILLSESQERMLIVAEKGKEHLINAVFE
                     KWDLNCTCIGEVVKGEQLKFYMNETLVADVNAESLVLGGGAPVYKRDYKEPAYFKQNK
                     TFNIDNVKVPIDLTAVAKQLASHPNICSRRWVYTQYDSMVGTINMSTNKPSDAAIVNI
                     KNSKKALAVSVDCNARYVFADPEQGCAIAVAEAARNIVCTGGEPSAVTNCLNFGNPYN
                     PEVYWQFVHSIKGMSKACEKFQTPVTGGNVSFYNQSKIGNDDVPVYPTPTIGMIGIIS
                     DKNKAATLAFSNAGDQIFLIGTSKNCIASSQYLVSTHGINNSPAPEFNLDEEYDVQQL
                     IKQLIKTGYVQSVHDVSDGGLFITLLESAMIKGLGFEINSDEDIRKDAFLFGESQSRV
                     VVSVSEEQMDEFISLVADSGVDFTNLGVVTHGEIIVDEVKFGNISEYSKLYQEKLESE
                     MAY"
     gene            2974..3600
                     /locus_tag="PKX59_04940"
     CDS             2974..3600
                     /locus_tag="PKX59_04940"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HOZ90247.1"
                     /translation="MYIPEYFRNTNFNQQVDFIKENSFGILITHSATEMFATHIPMTP
                     LVTNSNLVLHGHISKENPQCSTLINAGETLTIFSGPHCYISSSWYSHENVSTWNYQAI
                     HCYGKLKIQTELELLQSLIQLTDHYESKEEKPKFFEKIDPKVIRDNLIGIIGFEITVS
                     RIDAKDKLSQNRNKKDYENIVSHLCKRNDSMSDAIAEAMIKNADNANK"
     gene            3664..4539
                     /gene="era"
                     /locus_tag="PKX59_04945"
     CDS             3664..4539
                     /gene="era"
                     /locus_tag="PKX59_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOZ90248.1"
                     /translation="MHKAGFIAIVGKPNAGKSTLLNTLLGQDLSIVTHKAQTTRHRIK
                     GVITQPDYQLVFSDTPGIITPGYQLHQRMMTFVQSAFTDADVILLVYDATGNDMDAEK
                     FRTLLPAGKKLVVALNKIDLSNQEELEKIVQKIEQQLSPDAIVPISAKEKFGGEVLIK
                     TLLQFIPEAPPYFDKDTLTDRNERFFAAEIIREKIFLQYDKEIPYSVEVSIEEFKDTP
                     KLAKIKSYIFVERESQKAILLGKNGLAIKKLGTDSRKKIEEMTGKKVFLELTVKIRED
                     WRNNEQQLNKFGYNE"
     gene            4549..5868
                     /gene="der"
                     /locus_tag="PKX59_04950"
     CDS             4549..5868
                     /gene="der"
                     /locus_tag="PKX59_04950"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOZ90249.1"
                     /translation="MSNIIAIVGRPNVGKSTLFNRLTESMDAIVHDESGVTRDRHYGT
                     GEWTGRSFSVIDTGGYVSGSEDIFESEIRKQVKLAIEEADIILFVTDAREGLNDFDED
                     IAQLLRNSNKKTFLVVNKVDSPKQLNDAVEFYALGLGDYYCISSSNGSGTGELLDAVV
                     KVFKPDDEEEDELPRITIIGQPNVGKSSLMNMLTGVERSIVTPIAGTTRNSINIRYSN
                     FGFDFILVDTAGLRRKAKVKEDLEFYSVMRSIRAIEQSDVCLFMIDAEKGFTAQDANI
                     FHLIQTNHKGVVFLVNKWDLIDKENHSTKKFTEEIKAVIAPFTDVPIIFTSVLNKQRI
                     FKALETAIDVFKNRTQHIPTSQLNKKILPFIENYPPPSVKGKLINIKYITQVPGPYPR
                     FVFFSSNPQYIQTSYRRFLENKLRENFNLSGVPVAISFRSKDSGKEV"
     gene            5881..6645
                     /locus_tag="PKX59_04955"
     CDS             5881..6645
                     /locus_tag="PKX59_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOZ90250.1"
                     /translation="MNHHELKLYLDDLFLRYNRSDFIADDPISIPHKFSDSGDIEISA
                     FLTSLISWGQRKTILKNAQTLMEMMDHTPKSFIINASNNDYIPFKKFVHRTYNGEDCI
                     YIIKAIKNIYAEYGSLGNCLTSFVSNNPMPVAMSLFRQKLFSQPCQPRTTKHLGDPLK
                     NSSCKRFNMFLRWMIRKDRAGVDFGIWEISPSLLYCPLDVHTGRTARLLGLLKRQQDD
                     WKAVEELTINLRQFDKSDPAKYDFALFGAGVNGLLK"
     gene            6832..9525
                     /locus_tag="PKX59_04960"
     CDS             6832..9525
                     /locus_tag="PKX59_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOZ90251.1"
                     /translation="MNKLKIGILFGGSSREREVSFAGGRTVYDNLDKTLFEPIPLFVD
                     SFGNLLILDWQFIYKGSIRDFYPPSEFLPETTRGFQIYAENLGTLTHQEQIRLTMKLG
                     TRIEFAQLHDVIDFAFLCLHGSDGEDGRIQGMLEYYRIPYSGSGILSSAIGMNKVIQK
                     ELMKKSGFNVPEVAVINRIEWLASPNRKSFIKNLKSVGFPCVVKAANQGSSIGISVLK
                     NNDVEEFIQAVNKSFFIREITRTEWNSMTFDTKTEWIKKVSDIREGIGLPAKINDRTI
                     YHPEELLGVLIQLFGEMEDVVSIAAIEAETEVIIESFISGKEFSCIVITNENEKPVAL
                     PPTEIRKGNELFDYRAKYLPGLSRKVTPIHLPEVQIETIRRECERLFTSLNFSVYARI
                     DGFITENGTIFLNDPNTTSGMMPSSFFFHQAAEIGLNPSQFISYIISTSLQARLLEMK
                     SFRTVPQLIKNLHGLLSKKAKSQSEKIKVGVIMGGYSSERHISVESGRNVYEKLSSTE
                     KYEPIPYFLAGNEQSYSLYRIPINLMLKDNADDIREKILNYNQHPVIKKIIDQCGQVT
                     QTFANAANINPPEKTSFEELAQQVKIVFIALHGRPGEDGELQLQLNKVGLPFNGSRAE
                     SSKITIDKFQTNELLAQNGFKVAQHYKLTEEEWRNNPSAIEEKIQKEFGFPIIAKPVD
                     DGCSSAVKIIRNENQLHAYAELIFREDETLNTAAAELLKIKDKEEFPRKRYFLIETLI
                     SGENTKHFLEITGGMLTSYDEAGNIHYEIFEPSETLHEGDVLSLEEKFLAGEGQNITP
                     ARYSKHAMENRLISDIIRKELLRAARLLNIEGYARIDAFVRITIDNKVEVVFIEVNSL
                     PGMTPATCIFHQAAINQYKPYEFIEKILEFGFKRNQLSLTK"
     gene            9530..10291
                     /locus_tag="PKX59_04965"
     CDS             9530..10291
                     /locus_tag="PKX59_04965"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOZ90252.1"
                     /translation="MKELIRFLKSRIFWINALVIIIVIIMSIGLVYKWLDSFTDHGNS
                     VSVPDLKGMNIKKVDDFLKTKNLSFKIADSSVYLLDQKPGTIVEQDPQPNEKVKQGRT
                     IYLTITRSSAPMVKVPALKDVSLRQAEAILAASGLRMGEQIFKPDLAKNAVLSMMING
                     KDLKAGTDVPKGSAIDLIVGDGLGNTVVTVPSLIGLTYDEALFVLKGSSLNIGSLFFD
                     GVIKDTLNAKIYDQNPAPDNNTINQGEAIDLYLRP"
     gene            10299..11387
                     /locus_tag="PKX59_04970"
     CDS             10299..11387
                     /locus_tag="PKX59_04970"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOZ90253.1"
                     /translation="MIQLKSQEVINAPGRNSSVDIFRSFAILSVVIYHYNRQLPFGYL
                     GVDLFFVISGLLIGNILIGYLKKGEKINFFKFIIQRGFKIWPSYFSYLFIGSIIANVL
                     YHTLAPDMIIPADKYLRYILFYVNYTGNEVWSFGHLWSLCVEEHFYIMLPLLLILIQR
                     FFPDKKMLVTAILLVITAGVIFKFLVMAFTNSKDTYSATHNRIDALGWGVMLSIILNF
                     YPKLIVKQSTQITMLIAGIILFSLLLYTYSAYEWITFRKCVFHSAIPFCFFLMLAATY
                     HWNMSGLNWLRFIAYYSYNWYLWHPLWVTWFNKKIGTGLMAHLVFLIFTFLTAMAFTI
                     FIEEFFMGKRNKFISKWFGERKIQVKTL"
     gene            complement(11395..13335)
                     /locus_tag="PKX59_04975"
     CDS             complement(11395..13335)
                     /locus_tag="PKX59_04975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ90254.1"
                     /translation="MKLSVVIVNYNVKYFLEQCLISVGKAISNIDAEVFVVDNNSVDG
                     SCQMVRDKFPFVNVIENKINTGFSKANNQAIVQSKGEYLLLLNPDTVVEEDTFKKVVD
                     FMDNHPQAGGLGVHMIDGNGKFLPESKRGLPTPAVAFYKIFGLSALFPKSKLFGQYHL
                     GFLDKNKTNEVDVLSGAFMLLRKSVLDKIGLLDETFFMYGEDIDLSYRITKAGYKNYY
                     FPETKIIHYKGESTKKSSVNYVVVFYKAMSIFAQKHFSQSNAAFFSFLINIAIYLRAG
                     IALMNRLFSILLLPIIDAAFIYGGYTIIKTYWELYVKQLHYPEHYMLLVVPGYVLVWI
                     FAAYLNGGYDKPLRISRVVRGILSGTIFILVVYALLNETYRFSRALILMNTAWACLIT
                     ALIRYTLNAAGIKGFALAADVKKKLIVVADAVDAKRILSLLQLSGSNTTFVGYVDSGV
                     TQDGELKNFRLGTLTQLNEIITIYQVDEVIFSSHSLSSQQIIENMGALRNRQVEYKIA
                     PPESLFIIGSNSINDNGDLYLVDVNTLHSPSNRRKKRFFDIFSAFGILFCYPFLWLLF
                     KKNQNVFVNSIAVLFGKKTWVATNIKSLTSTHAKVGVYTPSDGLNTPSDNEYFRDRLN
                     VLYTRDYNMINDWNIVRRNIFK"
     gene            complement(13354..14319)
                     /locus_tag="PKX59_04980"
     CDS             complement(13354..14319)
                     /locus_tag="PKX59_04980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ90255.1"
                     /translation="MDISVVIPLFNEDESLPELAAWIDRVMKQHKFSYEVIMVDDGSK
                     DHSWQVIEDLNKQIPEIKGVKFKRNYGKSAALHKGFEMAQGHVVITMDADLQDSPDEI
                     PGLYKMIVEDEYDLVSGWKKKRHDPISKTIPSKFFNGVTRMVSGIDNLHDFNCGLKAY
                     HHNVIKNIEIYGEMHRYIPVIAKWVGFTKIGEKVVEHRARKFGTTKFGLERFVNGFLD
                     LLSITFVSRFGKRPMHLFGTLGSLMFILGLIGACYLGVSKLYCVYNDIPATRLTQRPS
                     FYLLLTSMILGTQLFLAGFLAEMIARNDKDRNRYIVEKEISNTTT"
     gene            complement(14339..14857)
                     /locus_tag="PKX59_04985"
     CDS             complement(14339..14857)
                     /locus_tag="PKX59_04985"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOZ90256.1"
                     /translation="MFRYGLHYGGILGASSFAMFLIIYLTGNNPLGNASWLGAWIPAV
                     VIAVAVKSYRTHEGEGFISYGEGFRTGLITALFGGILSALLIYIFCTLFDSTLVERFK
                     TDSLAQLELMEGQFKSMLSDKMYDQAVDAYNNISLQSIAMNDFYNKLLSGIILSLIIA
                     AVYKRNKPQILE"
     gene            14934..15032
                     /gene="ffs"
                     /locus_tag="PKX59_04990"
     ncRNA           14934..15032
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKX59_04990"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(15044..16339)
                     /locus_tag="PKX59_04995"
     CDS             complement(15044..16339)
                     /locus_tag="PKX59_04995"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOZ90257.1"
                     /translation="MKHFIFYLILFFLWSNSNVFGQEKQEYFKDNYFRYDNFTYRSNI
                     KTIQLENAQFPMSEPVIELNSGAQLLLQFDDLEGDYKDYWYTIIHCDAQWKPSDITRS
                     TYLFSFYEDRIADYAFSFNTLQPYTHYSLKFPNEQIKPLISGNYLLKVYLNNDPDSVA
                     FTRRFLVYENLIDVDATVHAATLAEYRQQKQEVDFSLSTTRYTIANPFGDLKVKILQN
                     FRWDNAVENLKPIFIKDNLLEYNYDEENTFNGSNEFRRFDIRSLKYETESVERIDHFD
                     TGTNVLLFPDKVRYFSRYTTDYDIDGKFEIKNYSGSHPERDADYATVRFRLEYKDEVK
                     DGNIYIFGALTDWGFTSQNMLRYDEDLKAYTATLFLKQGYYNYMYVFLKDGTSFGDES
                     WLEGNHYETENNYWILVYHRPQTERYDKLVACRKFASNR"
     gene            complement(16347..18251)
                     /locus_tag="PKX59_05000"
     CDS             complement(16347..18251)
                     /locus_tag="PKX59_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOZ90258.1"
                     /translation="MLVLNNISFDFGGRYLYHDAFWHIKPNQKIGLIGANGTGKSTLL
                     RIINGEYTLTGGEITGRKDLTVGFLNQDLLSYESDKSILDVALEVFERENILHQKIEK
                     ILKQLETEHGEELLNELFFLQSEFESLDGYNITHRAESILEGLGFSTIDLQRPLKEFS
                     GGWRMRVMLARMLLKKPSLLMLDEPTNHLDLPSIQWLEKYLQDYDGTFILVSHDRYFL
                     DRTINMIAEVANEKINLYPGNFTNYLEQKTMRDELQQRKFENQEKYIKEQQKLIDRFK
                     AKASKAKMAQSRMKMLDRMEKVEAVSGPASTMRLSLQVETQPGKVVFEGHHLNKNYGD
                     LDIFKNSHFTIERGDKIALIGANGKGKSTLLRMIAGTEKADGEIKEGYNVKTSFYAQH
                     QLESLNIENDLMRELQIFAPLETDQRIRSILGAFLFNRDDVFKKIRVLSGGEKARVAL
                     AKLILSKANFLLLDEPTNHLDMQSVEILTDVLLDYEGSFVVVSHDRFFVEQIANKIWF
                     IENNQLKEYPGTYEEYEHWAERKKSEKRTEEKQVVAKPVAPKVQAEPDQKKQLLNLEK
                     EVKKLEAELAAAKKQRNDLMEQLGNESNYNDTAKIKLLQKEFDESEKQYNKIHSLWED
                     SYMQWMEMSE"
     gene            complement(18439..20316)
                     /locus_tag="PKX59_05005"
     CDS             complement(18439..20316)
                     /locus_tag="PKX59_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOZ90259.1"
                     /translation="MFLFERTAPRWVVLVIDLILCTLSFYLAYDLRFNFTIPDVEIQL
                     LPTAFATVLSVRLVSFLISRTHTGIIRYTSYKDSQRIFLTLLSGTVLLCIFNLISFRI
                     YGRYFTPYSILMIDFFLSMLALASMRVIVKSAHVEWTRKKGNKTDVLIMGAGEEGMVV
                     KHALEQDAGINFKVLGFIDDKPKLRKRKLEGIPVFYTKTDLEDLLRANKISSLIIADK
                     DVDTKRINELIEICVKYDTKVQRAPRAGNWINGKLSFSQIKEVNIEDLLERDPINLDV
                     KEIRNQLNGKTILITGAAGSIGSEIARQVILYNPAKVVLLDIAESPLYELELSIQEIK
                     HAGNCESVIADIRNIDRMRRVFDYFKPEIVFHAAAYKHVPVMEVNPSEAILTNVKGTK
                     ILADLSVEFGVQKFVMISTDKAVNPTNVMGASKRVAEIYIQSLSEISETQFITTRFGN
                     VLGSAGSVIPRFKKQIENGMPVTVTDENITRFFMTIPEACQLVLEACKMGKGGEIFIF
                     DMGKPVKIAELAKKMIRLSGLEPEKDIKIQYTGLRPGEKLFEELLNKQENTIATHHPK
                     ILIAKVRKYKFDQIASDIENLTNLFKTQNNMALVSKMKEIVPEYISNNSEFEKLDRRE
                     I"
     gene            20600..20881
                     /locus_tag="PKX59_05010"
     CDS             20600..20881
                     /locus_tag="PKX59_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOZ90260.1"
                     /translation="MKSEIHPKNYRMVVFKDISCDYAFLTRSCVDTKDTIKWEDGNEY
                     PLVKLEISNMSHPYYTGKMKLIDTAGRVDKFRNRYAKSTAKKAAKTTEK"
     gene            20983..21867
                     /locus_tag="PKX59_05015"
     CDS             20983..21867
                     /locus_tag="PKX59_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOZ90261.1"
                     /translation="MITDKKSDAWISVVDESINTDVSRIMNLFIQCSSNFFSAVLIDT
                     DRNKFVALFDYRFPYPLSNDDLTEKILQIFDQNKPLLDNHYDSVVLSFCGFNNTLVPD
                     VFFDADRLDEFYYANHQHESTETTNKLLFDNLRRISARNIYSVPESLFDFFNSWYSNL
                     SVFHGATPLIEGVLTLHKNTSEKTVTVNIQNTYFEMVVNQGGNLLYYNKFNFQSTEDF
                     VYFLVFVYEQLEMNPESQVLNIAGTAEKNSALIVLASKYIRHIQFVQRPSFAFYSYGF
                     DEVAAHYHFSLYNQFLCV"
     gene            21879..22397
                     /locus_tag="PKX59_05020"
     CDS             21879..22397
                     /locus_tag="PKX59_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOZ90262.1"
                     /translation="MRGKKITAPDNLPVRPTTDFAKTGLFNILSNHLNFSTVTALDLF
                     TGTGNISYELISRGVKNVTAVDADKRCIAFVQKIFNDIHAENAEAIQSDALEFLEQCS
                     RTFDLIFADPPFNSNPIQDIVEIVFRKKLLTSQGLLIIEHSSEMQAPKSDNLLETRTY
                     GAVAFSIFVAEK"
     gene            22406..22867
                     /gene="coaD"
                     /locus_tag="PKX59_05025"
     CDS             22406..22867
                     /gene="coaD"
                     /locus_tag="PKX59_05025"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOZ90263.1"
                     /translation="MKTAVFPGSFDPIHNGHLEIVQRMLPLFDKIIIAIGINPDKKYL
                     FSLEQRLNWLEQVFKNESKINITSYHGLTAEFCKQQQAQYMIRGLRNSSDFEFEKAIA
                     QMNKKLGGIETVFVISSPENAPLSSTILRDIIINGGSVEGLVPVKIENQKK"
     gene            complement(23079..23819)
                     /locus_tag="PKX59_05030"
     CDS             complement(23079..23819)
                     /locus_tag="PKX59_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90264.1"
                     /translation="MEEKYIKWATITIFVCGGLILIANYAKDYFTKEKARKEKEAAQR
                     ENAMQGEIVAPESKPAPHSQAAQLPEEMVELKIGGMTCAFKKSQFEAGVETPKCLQVG
                     NTVPVIIKLKGGKLFISTKIFDKAGKMVCEIDDNEWTVNKNNFYKRNYDSTAVEVIDQ
                     YDNIVLSAEVKNKNSIHVYGIFNAIGQAFIFAYGDVTETLEYFPQAIKIIEQMEDRPW
                     DELYREKAKKIPRVFLYNGKDYLGKRVS"
     gene            24298..24915
                     /locus_tag="PKX59_05035"
     CDS             24298..24915
                     /locus_tag="PKX59_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90265.1"
                     /translation="MKNVTTLICTFLLLSSSAFAQTSYPKGGYKSFEEFQNKQPSIPF
                     VFEITRRTKGDIKMNGGVDYKVTSDSISKSTIKRDIYAISSGDTLYMNCFTHKVQFWY
                     AKVLVEGKYIAFEGGIPMDKNSGVVAAGVAFGPLGGGIAGAHAAMLRYLYIMEAAVGK
                     IKQFDSKYLITLLESFPDLKNQYLNEPKQADEETLLKYLRLINSN"
BASE COUNT         8407 a         4726 c         4562 g         8067 t
ORIGIN      
        1 agtaaagagc cattcctgtt gcaaacaata tcagtgaatc tttcaacaat ccagaccccc
       61 aaaataaaac agaaggaata aaaaatactc cggcaaacaa aactctcctg agttccggca
      121 gtgcttttac aaaaagtttg agcaatgcag tgagtccgac aaatgacaag aaacaataaa
      181 acactgcatg tacatgataa tagcccaaac tgatgaaacg catcaacgca ttcaatctaa
      241 tctgaaaacg atagtcatta aacagaacta ctttattgaa ccaattgtgc atggtatcat
      301 aatatggcat caggtaactt gcctgatcat taataccaaa aactgcctgc agaaaccaaa
      361 gtgggtgctc aaaagcaagg tcatataata tcttgctatc atcaaaaaac ttaaaggtat
      421 ctgccgtcag tctgtctgta taatattttg aatagattac ccctaataaa atggcaaaaa
      481 ttattttaac aagaaacgct gctttaagcc accataccgg aattccttcg atcctgaaaa
      541 atgccgattt atgaatgaca aaaacaaata acgctatgta tccaatgcta agaaccgctt
      601 ccaaagtaag gttgtttgaa aattttcata caatacaata acctgtttat tacatcaata
      661 ggttaccttt gcgcaaagta acataaaagt attctgaaac gaatttatac tttaaaaata
      721 aagaatggaa agtttgaaag aaacaacggt tgaaacagca aaaaaattag gtcttttacc
      781 ggaagagttt gataaaatct gctcactaat gggtcgtaag cctaatttta atgaattgag
      841 tatttactct gtgatgtgga gtgaacattg ctcttataaa aattctatta cctggctgaa
      901 aactttgcca cgcgaaggac ctcacatgtt agccaaagca ggagaagaaa atgcaggtct
      961 tgttgatatt ggcgacaatc ttgcctgtgc atttaaaatt gaatcgcaca accacccttc
     1021 tgccatagaa ccctatcagg gagctgcaac aggagtgggt ggaattaacc gtgatatttt
     1081 tactatgggt gcaagaccta ttgcacaact taactctttg cgttttggca atctgaataa
     1141 tgagcgcaca aaaatgctta tgcgcggtgt tgttaaagga attggtgact atggtaatgc
     1201 ctttggtgtt ccaacagttg gtggcgaagt ttattttgaa gattgctaca atacaaacat
     1261 tttagtcaat gccatgagtg taggaattgt tgagcatggc aaaactgtaa aagcaatatc
     1321 agaaggcgaa ggcaacagtg tttatattgt aggctcagca acaggaaaag acggtattca
     1381 tggtgccact tttgcatcgg gcgacctgca tgaagattcg catgaggatt taccatctgt
     1441 gcaggtaggc gatccatttc aggaaaaact tttattagag gcaacacttg aagtcattgc
     1501 aaccggtgca gttgtaggaa tgcaggatat gggtgctgca ggaattattt gttctacctc
     1561 agaaatgtct gccaaaggaa atcatggcat gattatacac cttgacaaag ttcctacgcg
     1621 ccaaaaaaac atggaacctt gggaaatttt actttccgag tcgcaggaaa gaatgctcat
     1681 tgttgcagaa aaaggtaaag aacatttaat taatgcggtt tttgaaaaat gggatttaaa
     1741 ctgcacatgc attggcgaag tagttaaagg cgagcaatta aaattctata tgaatgagac
     1801 gttggttgca gatgttaatg ctgaatcact tgtattaggt ggcggtgctc ctgtctataa
     1861 aagagattat aaagagcctg catacttcaa acaaaacaaa acatttaaca ttgataatgt
     1921 aaaagttcct attgatctta ctgccgttgc caaacaatta gcatcacatc cgaatatttg
     1981 tagcagacga tgggtttaca cacaatacga cagtatggtg ggcacaatta atatgagtac
     2041 aaacaaacca tctgatgctg caattgtaaa tattaaaaat tctaaaaaag cacttgcagt
     2101 ttctgttgat tgtaatgcaa gatatgtttt tgcagatcct gaacaaggct gcgctattgc
     2161 agttgcagaa gcagcacgca atattgtctg tacaggagga gagccttctg ctgttaccaa
     2221 ttgccttaac tttggcaacc catataatcc tgaagtttac tggcagtttg tgcatagcat
     2281 aaaaggtatg tcaaaagcat gcgaaaaatt tcaaactccg gtgacaggtg gtaatgttag
     2341 tttttataat cagtcgaaaa tcggaaatga cgatgtcccc gtttacccta ctccaactat
     2401 tggcatgatt ggaattatca gtgataaaaa caaagctgca acattggcat tttcaaatgc
     2461 aggtgatcag atatttctga ttggcacttc taaaaactgt attgcttcat cacaatatct
     2521 tgtcagcaca catggtatca acaacagccc tgcgcctgaa tttaatctcg atgaagaata
     2581 tgatgttcaa caactcatca agcagttaat caaaacagga tatgtacaat ctgtacatga
     2641 tgtttctgat ggtggattat ttattacttt acttgaatct gcaatgataa agggtctggg
     2701 ttttgaaatt aactctgatg aggatataag aaaagatgca tttctttttg gagaatcaca
     2761 gagtcgtgtt gtagttagtg tttctgaaga acaaatggac gaatttatca gcttagtagc
     2821 tgattcaggc gtggacttca ccaacctggg tgttgtgaca catggtgaaa taattgttga
     2881 cgaagttaaa tttggcaaca tcagcgaata ttcaaaactc tatcaggaaa aacttgaaag
     2941 tgagatggct tactaaaagt attcttttca attatgtaca ttcctgaata ttttcgaaat
     3001 acgaatttta accaacaagt tgactttatt aaagaaaaca gttttggaat tttaataact
     3061 cattcagcaa ctgaaatgtt tgctacccac attccaatga cacctttagt aacaaacagc
     3121 aatcttgtat tacatgggca tatatctaaa gaaaatccac aatgcagtac tttaattaat
     3181 gctggtgaaa cactaacaat ttttagtggt ccgcattgct acatttcatc ctcatggtat
     3241 agtcacgaaa atgtttctac atggaattat caggctatac attgttatgg gaaattaaaa
     3301 attcaaacgg aattagaact gttgcaaagt ctgatacaac ttaccgacca ttacgaatct
     3361 aaagaagaga aacctaaatt ttttgaaaag attgacccga aagtaatcag agataatctg
     3421 atcggaataa ttggatttga gattacagtt tcaagaattg acgcaaagga taaattgtcg
     3481 caaaacagaa acaaaaagga ttacgaaaat attgtttcac atctctgcaa acgcaatgat
     3541 tccatgtctg atgccattgc cgaagccatg attaaaaatg ctgacaatgc aaacaaataa
     3601 caccgaattc ggctaactga aaaaattgcc cccttttctt taccttcgca ggaaattttt
     3661 aaagtgcata aagcaggatt tatagctata gtaggaaaac ctaatgcagg aaaatcaacc
     3721 ctattgaaca cacttttagg gcaggatttg agtattgtta cccataaagc tcagaccaca
     3781 cgtcaccgca taaaaggtgt gataacacag cctgattatc aattggtgtt ttctgataca
     3841 ccgggcatta tcacccctgg atatcaatta catcaacgaa tgatgacctt tgttcagagt
     3901 gcatttacag atgccgatgt aattttattg gtgtatgatg ctaccggaaa tgatatggat
     3961 gccgaaaagt tcagaacact gctgcctgcc ggaaaaaagt tagttgtagc actgaataaa
     4021 attgatttaa gtaatcagga agaacttgaa aaaatagttc aaaaaatcga gcaacaactt
     4081 agccctgatg ccatagtacc tatttcagct aaagaaaaat ttggtggaga agttttgatt
     4141 aaaaccttat tgcaatttat tcctgaggca cctccttatt ttgacaaaga cacactgact
     4201 gacaggaatg aaagattttt tgctgccgaa ataattcgtg agaaaatttt ccttcaatat
     4261 gataaagaaa taccctatag tgttgaagta agtattgaag aatttaaaga cacccctaag
     4321 ctggcaaaaa tcaagagtta tatttttgtt gaaagagaat cgcagaaagc aattttatta
     4381 ggaaaaaatg gcttggctat aaaaaagttg gggactgaca gcagaaagaa aatagaagag
     4441 atgactggca aaaaagtatt tctcgaactg acggtaaaga ttagagaaga ctggagaaat
     4501 aacgagcaac agctgaacaa attcggctac aacgaataac aaagaaaaat gagcaacatt
     4561 attgctattg taggcagacc caatgtaggg aaatcaacac tgttcaacag gttgacagaa
     4621 agtatggacg ccattgtaca tgatgaatcg ggtgtaacac gcgacagaca ctacggtaca
     4681 ggtgaatgga cagggagaag tttttctgtg attgatacag gaggctatgt atcgggctca
     4741 gaagatattt ttgaatctga aataagaaaa caagtcaagc tggcaatcga agaagcagac
     4801 atcatccttt ttgttacaga tgcgcgcgaa ggcctgaatg attttgatga ggatattgca
     4861 caattacttc gcaatagtaa taagaaaaca tttttagtag ttaacaaagt tgattcccct
     4921 aaacaactga atgatgcagt tgagttttat gcattaggtc ttggtgatta ttattgcata
     4981 tcatcatcaa acggtagtgg cactggcgaa ctactagatg ccgtagttaa agtttttaag
     5041 cctgatgatg aggaagaaga tgagttgcca agaatcacaa ttattggtca gcctaatgtc
     5101 ggcaaatctt ctttaatgaa tatgcttacc ggtgtggaac gcagtatcgt tacacctatt
     5161 gcaggaacta ccagaaatag tataaatatt cgttattcaa attttggttt tgattttatt
     5221 ttagttgaca ctgcaggatt gagaaggaaa gccaaagtga aagaagattt ggaattctac
     5281 tctgtaatgc gcagcataag agccattgag caaagtgatg tgtgtctgtt tatgatagat
     5341 gcagaaaaag gattcacagc acaggatgca aatatttttc acctcattca aaccaatcac
     5401 aagggtgttg tgttcttagt caacaaatgg gatttaattg acaaagaaaa ccattcaaca
     5461 aagaaattta cagaagaaat aaaagctgtc attgctccat ttactgatgt accaataatc
     5521 tttacttcgg tactaaacaa gcaacgtatt tttaaagcac ttgaaactgc tattgatgtt
     5581 ttcaaaaaca gaacgcaaca tattccgaca tcgcagctta acaaaaaaat actacctttc
     5641 atagaaaatt atcctccacc gtccgtcaaa ggtaaactca tcaatattaa gtatattact
     5701 caggtgcccg gaccatatcc acgttttgtc ttttttagta gcaatccgca atacatacaa
     5761 acttcgtaca gaaggtttct tgaaaataaa cttcgcgaaa atttcaatct gagtggtgtt
     5821 cctgttgcaa tttcttttag aagtaaggac agtggcaagg aagtctgaaa acagaaaatc
     5881 atgaatcatc atgagctcaa actgtattta gacgatttat ttctgcgata caacagaagt
     5941 gattttattg ctgatgatcc aatttctatc cctcataagt tctctgacag tggtgatatt
     6001 gaaatcagcg cctttctgac ctctcttatc tcatgggggc agcgaaaaac cattctgaaa
     6061 aatgcgcaaa cacttatgga aatgatggat catactccaa aatcatttat cataaatgca
     6121 tcaaacaacg attatatccc ttttaaaaaa tttgtgcacc gcacttataa tggagaagat
     6181 tgtatctaca ttattaaagc tattaaaaat atttatgctg aatacggatc gttaggaaat
     6241 tgcttaacat cttttgtaag caacaatccc atgcctgtag ctatgagttt attcaggcag
     6301 aaactattct cccaaccctg ccaaccacgc accaccaagc atttaggcga cccattaaaa
     6361 aattcaagtt gcaaaagatt caatatgttt ctacgttgga tgataaggaa agaccgtgcc
     6421 ggtgttgact ttggaatttg ggaaatatca ccctcattac tttattgtcc tttagatgta
     6481 cataccggtc gaacagcacg actacttggg ctgctgaaaa gacagcaaga tgattggaaa
     6541 gccgttgaag aactgactat aaatcttcga caatttgata aaagcgaccc tgcaaaatac
     6601 gactttgcac tttttggtgc aggtgtcaat gggttgttaa aataagctct ggatatcagt
     6661 cgtttttatt gagcaaaaac caatttaaaa aacaatcttt ctaacgtgta tttaataaaa
     6721 actgctttca aaataaatgt taaaaaaata gtgaatactt actcttatag catgtatgct
     6781 gctctattaa tctgttaact ttgttcaatc atttcatgac ttaaaattta tatgaacaaa
     6841 ctaaaaatag gaattctttt tggcggctca tcacgcgaac gcgaggtgtc ctttgccgga
     6901 ggcagaaccg tatatgacaa cctcgacaaa acactatttg aacctattcc tctttttgtt
     6961 gacagttttg gcaacctact gattctcgac tggcaattta tatacaaggg tagcattcgt
     7021 gatttttatc ctccatcaga atttttaccc gaaacaacac gtggttttca gatttatgca
     7081 gaaaatcttg gaacgctcac tcatcaggaa caaataagac taacgatgaa gttaggaacg
     7141 cgcatagagt ttgcgcagtt acatgatgta attgattttg cattcttatg tctgcatggc
     7201 tctgatggcg aagatggacg catacaagga atgttggaat attaccgaat cccttactca
     7261 ggttcaggta ttctttcttc tgcaatagga atgaataaag ttattcagaa agaactgatg
     7321 aaaaaatcag gttttaatgt tcctgaagtt gctgtcataa accgaataga atggcttgcg
     7381 tcaccaaacc gaaaatcttt tattaaaaat ttaaaatctg ttggatttcc ttgtgtagtt
     7441 aaagctgcta atcagggttc gagtatcgga atatcagtat tgaagaataa tgatgtagaa
     7501 gaatttatac aagctgtcaa caaatcattt tttatccgcg aaatcactcg gacagaatgg
     7561 aacagcatga cttttgatac aaaaacagaa tggataaaga aagtatctga tattcgcgag
     7621 ggtattggtt taccagcaaa aattaatgat agaacaatct atcacccgga agaattattg
     7681 ggagttctga tacaactttt tggcgaaatg gaagacgttg tttctattgc tgctattgaa
     7741 gctgaaacag aagttattat tgaatcattc atttcaggaa aagagttttc atgcatcgta
     7801 attacaaatg aaaacgaaaa gcctgttgca ttaccgccaa cagaaatacg caaaggcaat
     7861 gagttgtttg actaccgagc taaatacctg cccggtttat caagaaaagt aacacctatt
     7921 cacctgcccg aagtacaaat tgaaacaata agaagagagt gtgaacgtct gtttacgtca
     7981 ttaaacttta gtgtttatgc ccgaatagat ggttttatta cagagaatgg tacaatattt
     8041 ttgaatgatc cgaatacaac atcaggaatg atgcccagta gctttttctt tcatcaggct
     8101 gcagaaatcg gtctcaaccc ttctcagttt atcagctaca ttatttcaac atcacttcag
     8161 gcgcgtctct tagaaatgaa atcatttcgt acggtaccac aactgataaa aaacctgcat
     8221 gggcttctca gcaaaaaagc caaatcgcaa tctgaaaaga taaaagttgg tgtcataatg
     8281 ggtggctata gcagtgaaag acacattagt gttgagagcg gacgaaatgt ttatgaaaaa
     8341 ttatcttcaa cagaaaagta cgaacccatt ccttattttc ttgccggtaa cgaacagtct
     8401 tattcacttt acaggattcc aataaacctg atgctaaaag ataatgctga cgatatcaga
     8461 gaaaaaattc tcaattacaa tcagcatcct gttattaaaa aaataattga ccaatgtggt
     8521 caggtaacac aaacgtttgc aaatgcggcc aatataaatc ctcctgaaaa aacatcgttt
     8581 gaagagctgg cgcaacaagt aaaaattgtt ttcattgctt tacatggtcg tccgggtgaa
     8641 gatggtgaat tacaattaca acttaataaa gttggtttac cattcaatgg ctcaagggct
     8701 gaatcttcta aaatcactat tgataaattt caaacaaatg agttgctggc acaaaatgga
     8761 tttaaagttg cacaacatta taaactcaca gaggaagaat ggcgtaataa tccatctgcg
     8821 atagaagaaa aaattcaaaa agaatttgga ttccctatta ttgctaaacc ggttgatgat
     8881 ggatgcagta gtgcagtaaa aattatccga aatgaaaatc agttacatgc ctatgccgaa
     8941 ttaatttttc gcgaagatga aacattaaac actgctgcag ctgaactact gaagataaaa
     9001 gacaaagaag agtttccacg aaaaagatat ttccttattg aaacacttat cagtggtgaa
     9061 aacactaaac attttttaga aatcacaggt ggcatgctca catcctatga tgaggccgga
     9121 aatatacatt acgaaatttt tgaacccagc gaaacattac atgaaggtga tgtactttcg
     9181 cttgaagaaa aatttttagc cggtgaagga caaaatatca ctccagcacg ttacagcaaa
     9241 catgcaatgg aaaacagact gatttctgat ataatcagaa aagaactttt gcgtgcagca
     9301 cgactgttaa atattgaagg ctatgcacga attgatgcat ttgttcgtat tacaatcgac
     9361 aataaagttg aagttgtttt tatagaagtg aattcattgc ccggcatgac accggcaact
     9421 tgcattttcc atcaggctgc aataaatcag tataagcctt atgagtttat tgaaaaaata
     9481 cttgaattcg gatttaaaag aaatcagtta tccctgacga aataaaataa tgaaagaact
     9541 aattcgattt cttaagtcgc gcattttctg gattaatgct ttggtgatta ttatcgtaat
     9601 catcatgtca ataggtttgg tttacaaatg gcttgacagt tttacagatc atggcaactc
     9661 agtttctgtg cctgatttaa aaggcatgaa cattaaaaag gttgatgatt ttttaaagac
     9721 taaaaacttg tcatttaaaa ttgctgattc atcggtttac cttttagatc aaaaaccggg
     9781 aactatagtt gagcaagatc cgcaaccaaa tgaaaaagta aaacaaggtc gtactatcta
     9841 tcttacaata actcgcagta gtgcaccaat ggtgaaagtt ccggcactga aagatgtttc
     9901 cttacgacag gcggaagcca ttcttgctgc atcaggactt agaatgggtg agcagatttt
     9961 caaacctgat cttgcaaaaa atgcagtgct ctccatgatg ataaatggaa aagatttaaa
    10021 agcaggaaca gatgtaccta aaggttctgc cattgatttg attgttggtg atggattagg
    10081 aaatacagta gtcactgttc catctcttat tggcctaact tatgacgaag cattattcgt
    10141 gctaaaagga tcatcattaa acattggttc attatttttt gatggtgtga ttaaagatac
    10201 gcttaatgca aaaatctatg atcagaaccc tgcacctgat aacaacacca tcaatcaggg
    10261 tgaagccata gatttatatt tgagaccttg attcttttat gattcaacta aaatcacaag
    10321 aggtaattaa tgcccctggc agaaattcaa gtgtagatat tttcagatca tttgcgatac
    10381 taagtgttgt tatttatcat tacaacaggc agcttccttt tggctatctt ggcgttgatt
    10441 tattttttgt gatctccgga ttactgatag gtaatattct tattggctat ttaaaaaaag
    10501 gggagaaaat aaattttttc aaatttatta ttcaacgagg gtttaaaatt tggccatctt
    10561 atttttcgta tctgtttata ggatcaatca tagcaaacgt tttatatcat acccttgccc
    10621 ccgacatgat aattccggca gataaatatt tacgttatat tctcttctat gttaattata
    10681 ccggtaatga agtatggagt tttgggcatt tgtggtcgct ctgtgtagaa gaacattttt
    10741 atatcatgct accactgcta ttgattctaa ttcaaagatt cttccccgac aaaaaaatgt
    10801 tggtgactgc aatcttattg gtaattacag caggagttat atttaagttt cttgtcatgg
    10861 cttttaccaa tagtaaagat acctattctg ccacacataa ccgcatagat gctttaggat
    10921 ggggtgtgat gctgagtatt attctcaatt tttatcctaa gttaatagta aaacaaagca
    10981 cacaaatcac aatgctgatt gcaggaatca ttctttttag tttacttctg tacacatata
    11041 gtgcttatga atggattacc ttcaggaaat gcgtgtttca ttctgctata cccttttgtt
    11101 tttttctgat gcttgcagct acttatcact ggaatatgtc aggattaaat tggttgagat
    11161 ttattgcata ctacagttac aactggtatt tgtggcatcc actttgggta acctggttta
    11221 acaaaaaaat cggaacaggt ttgatggcac atctggtgtt tcttattttt acttttctga
    11281 cagcaatggc atttactatt tttatagagg aatttttcat gggtaaaaga aataagttta
    11341 tcagtaaatg gtttggcgaa agaaaaattc aggttaaaac cttgtaatat tttctcactt
    11401 aaaaatattt ctccgtacta tattccagtc gttaatcata ttgtaatcac gggtataaag
    11461 cacatttaat ctgtcgcgga aatattcatt gtctgatggt gtgtttaatc catctgatgg
    11521 cgtataaaca ccaactttag catgtgttga cgttaaactc ttaatattgg ttgctaccca
    11581 tgttttcttt ccaaaaagta cagcaatgct atttacaaaa acattttgat ttttcttgaa
    11641 taatagccaa agaaaaggat agcagaataa tattccgaaa gcactgaaaa tatcaaaaaa
    11701 tctttttttt cttctattgg aaggactgtg taatgtattt acatcaacaa gataaagatc
    11761 accattatca tttattgaat tactaccaat gataaacaaa ctttctggag gagcaatctt
    11821 atactcaacc tgcctgttac gcagcgcccc catattctcg attatctgct gcgagcttaa
    11881 tgagtgagaa ctgaaaatta cttcatcaac ctgataaata gtaatgattt catttaattg
    11941 tgtcaatgtt ccaagtctga aattcttcaa ttctccgtcc tgagtgactc ctgaatcaac
    12001 atatcctaca aaagttgtat ttgaccctga taattgtagc aatgataaaa ttctttttgc
    12061 atcaacagca tctgcaacaa caatcaattt ctttttgaca tctgctgcca aagcaaatcc
    12121 tttaatacct gctgcattta acgtatatcg gattaacgca gttatcagac atgcccaggc
    12181 tgtattcatt aaaatgagcg ctcttgaaaa acggtaggtc tcgttcaaca gagcatacac
    12241 aactaaaata aaaattgtgc ctgacagaat tcctcttaca actctggaaa tacgcagtgg
    12301 tttatcataa ccaccattca gataagcagc aaaaatccag actaaaacat atccgggcac
    12361 aactaaaagc atgtaatgct ccggataatg caattgtttg acataaagtt cccaatatgt
    12421 tttaataatg gtataacctc catatataaa tgctgcatca ataattggta acaacaaaat
    12481 actaaaaagg cgattcatca atgcaatacc tgctcttagg tagatggcaa tatttatcag
    12541 aaatgaaaaa aatgcagcat tgctctgcga aaaatgtttc tgagcaaaaa tgctcattgc
    12601 cttataaaac actactacat aatttacact gctcttcttg gtactctctc ctttgtagtg
    12661 tatgattttt gtttcaggga aataataatt tttatagccg gcttttgtta ttctgtatga
    12721 caagtcaata tcttcaccat acataaaaaa tgtctcgtca agtaatccta ttttatccaa
    12781 cacagatttt ctcaacaaca taaatgcacc actgagcaca tcaacttcat tggtcttatt
    12841 cttgtcgagg aaacctaaat gatactgacc aaataatttt gattttggaa acaatgcaga
    12901 cagcccgaag attttataga atgccactgc aggtgtcgga agtcctcttt tggattcagg
    12961 caaaaatttt ccgtttccgt caatcatgtg cactccaagt ccgcctgcct gtggatgatt
    13021 gtccataaag tcaaccacct ttttaaatgt atcctcctct acaacagtgt ccggattaag
    13081 aagtagcaaa tactcacctt tgctttgtac aatagcctga ttgtttgctt ttgaaaaacc
    13141 ggtgtttatt ttattttcaa ttacgtttac aaatggaaat ttatcccgaa ccatctgaca
    13201 tgatccgtca acagaattgt tgtcaaccac aaaaacttct gcatcaatat tactaatagc
    13261 cttaccaaca gaaatgaggc attgctcaag aaagtacttt acattgtagt tgacgatgac
    13321 aacggatagc ttcaatgcaa cagaattttt gggttacgtt gttgtgttag atatttcttt
    13381 ttccacaata taacgattgc ggtctttatc attgcgagca atcatctctg caagaaatcc
    13441 tgcaagaaaa agttgtgttc ccagaatcat acttgtcagc aataaataaa atgatgggcg
    13501 ctgtgttaat cgtgttgcag ggatatcatt ataaacgcaa taaagttttg aaacaccaag
    13561 ataacaagca cctataagtc ccagaataaa cataagagaa cctaaagtac cgaataaatg
    13621 catggggcgt ttaccaaaac gtgaaacaaa tgtgatggaa agcaaatcaa gaaatccatt
    13681 aacaaatctt tctaatccaa attttgtagt accaaatttt ctggctctgt gttcaacaac
    13741 cttctcaccg atttttgtaa agccaaccca ctttgcaatt acaggaatat aacgatgcat
    13801 ctcaccgtat atttcaatat tcttaatcac attatgatga tatgctttaa gcccacaatt
    13861 aaaatcgtgc agattatcaa tcccggaaac catacgggtg acaccattaa aaaatttgct
    13921 gggaatagtt tttgaaatcg gatcgtgacg tttctttttc catccactca ccaaatcgta
    13981 ttcatcttca acaatcattt tataaagtcc gggaatttcg tcaggactgt cttgtaggtc
    14041 ggcatccatt gtaattacta catgaccttg tgccatttca aaacctttat gtagtgcagc
    14101 agacttgcca tagtttcttt taaacttcac accttttatt tcaggtattt gtttatttag
    14161 gtcttcaatc acctgccatg agtgatcttt gctaccatca tcaaccataa tcacttcata
    14221 gctaaacttg tgttgcttca taacacggtc aatccatgct gccaattcag gtagagactc
    14281 atcctcatta aacaacggaa taactactga aatatctatt ctgtatgatg actgcatttt
    14341 attcgagaat ttgaggcttg ttacgtttat acactgcggc aatgataagt gaaagtataa
    14401 ttccgctgag tagcttatta taaaaatcgt tcattgcaat tgactgaaga cttatattat
    14461 tgtaggcatc aacagcctga tcatacattt tatcactgag cattgattta aactgtcctt
    14521 ccataagttc aagttgtgca agagaatcag ttttaaaacg ctctaccaat gtactgtcga
    14581 ataaggtgca gaaaatataa atcaacaatg ctgaaagaat tccaccaaaa agagcagtaa
    14641 tcaatcctgt acggaaacct tcaccataag agataaaacc ctcaccctca tgtgtccggt
    14701 aactttttac tgcaacagca attacaactg caggtatcca tgcaccgagc cacgatgcat
    14761 tgccaagcgg attatttccc gtaagataaa taatcaaaaa catagcaaaa cttgatgccc
    14821 ctaatattcc tccataatgt aatccgtatc gaaacataat ttatgattgc aaaggtataa
    14881 aacctgctgt gtttatttgt ttattgaaag gtatgattta ctatctttgc accgggtaag
    14941 tcttatacga ccagctccct cgaacctccc cagggcagga atgcagcaag gataagtggt
    15001 cgtagcggtg cgatataagt agcttaccca tttttatttt aaactatctg ttggaagcaa
    15061 attttctgca ggccaccaat ttatcgtacc tttctgtttg cgggcgatga tataccaaaa
    15121 tccaatagtt gttttctgtt tcatagtgat tgccttcaag ccagctttca tctccaaaag
    15181 aagttccgtc ttttaaaaac acatacatat aattgtagta gccttgtttt aaaaacaatg
    15241 ttgctgtgta agctttcagg tcttcatcat aacgaagcat gttctgactc gtaaaacccc
    15301 agtcggtgag agccccaaaa atataaatgt tgccatcttt aacttcgtct ttatattcaa
    15361 ggcggaaacg aacggttgca taatcggcat cacgctcagg atggctgcca ctgtagtttt
    15421 ttatttcaaa ctttccgtca atatcatagt cggttgtata acgactgaaa taacgaacct
    15481 tatcagggaa aagcaacaca ttggttccgg tatcaaaatg atctattcgc tctacacttt
    15541 ctgtttcata ctttaatgag cgaatatcaa agcgtctgaa ctcattactg ccattaaaag
    15601 tattttcttc atcataatta tattccagca agttatcttt aataaaaatc ggcttcagat
    15661 tctcaacagc attatcccat ctgaaattct gaagaatttt aacttttaaa tcaccaaagg
    15721 gattggcgat ggtatatctt gtagttgaca atgaaaaatc aacttcctgt ttctgttgtc
    15781 tgtattcagc caatgttgct gcatgcacgg tagcgtcaac atcaatcagg ttttcataca
    15841 ctaaaaatct tcttgtaaat gcaacactgt cagggtcgtt attcaaataa actttcagca
    15901 aataatttcc tgatatcagt ggtttaatct gttcgtttgg aaattttaat gagtaatgag
    15961 tgtaaggttg caaggtgtta aacgaaaaag cataatcagc aatacggtct tcgtagaagc
    16021 taaacaaata tgttgagcgc gtaatgtctg aaggcttcca ttgtgcatcg caatgaatga
    16081 tggtgtacca gtaatcttta taatcgcctt caaggtcgtc aaactggagt aaaagctgcg
    16141 ctccactgtt gagttcaatc acaggttcac tcatcgggaa ctgtgcattt tccagttgta
    16201 tcgttttaat attacttctg tacgtaaagt tatcgtacct gaaataattg tctttaaaat
    16261 attcttgctt ttcctgacca aacacattag agttagacca caaaaagaaa agtatcagat
    16321 aaaaaataaa atgtttcata agcacatcat tcactcattt ccatccattg catgtagctg
    16381 tcttcccaca atgaatgtat cttattatac tgtttttcag attcatcaaa ctccttctgt
    16441 aacagtttga ttttagctgt gtcattataa ttactttcat tgcccaactg ctccatcaaa
    16501 tcattgcgtt gcttctttgc tgctgccaac tctgcttcca attttttaac ctccttctct
    16561 aaattcaaca actgtttttt ctgatcgggc tctgcctgca cctttggtgc aacaggtttg
    16621 gctacaactt gtttttcttc tgttcgtttt tcagattttt ttcgttctgc ccaatgctca
    16681 tactcttcat aagttccggg atattcttta agctgattgt tctcgataaa ccatatttta
    16741 ttggcaatct gttcaacaaa aaaacgatcg tgtgaaacca ctacaaaaga gccttcgtaa
    16801 tccaacaaca catcagtaag aatttctaca ctttgcatgt cgaggtggtt ggtaggctca
    16861 tctaataaca gaaaatttgc tttgctgaga attaacttcg ctaatgccac acgtgctttc
    16921 tcacctcctg aaagtactcg aattttctta aaaacatcgt cacgattaaa aagaaatgca
    16981 cccagaatac ttctgatacg ttgatcggtt tctaacggag caaaaatctg cagttcgcgc
    17041 atcaaatcgt tctcaatatt caaactttct aactgatgct gtgcatagaa agatgtcttt
    17101 acattgtagc cttccttgat ttcgccatct gctttttctg tgccggcaat catgcgcaat
    17161 aaggttgact taccttttcc gttagcacct ataagtgcaa ttttatcacc tctttcaatt
    17221 gtaaaatgac tgtttttaaa aatatccaga tcaccataat ttttattcag atgatgtcct
    17281 tcaaaaacta ccttgccggg ctgtgtctct acttgtaaac ttaatctcat ggttgatgca
    17341 gggccggaaa ctgcttcaac tttttccata cggtcgagca ttttcattct ggactgagcc
    17401 atctttgcct tgctggcttt tgctttaaag cggtcaatta atttctgttg ctctttaata
    17461 tacttttcct gattctcgaa tttacgctgc tgcaattcat cacgcatagt tttctgttcg
    17521 agatagttgg tgaaattacc cggataaaga ttaatttttt cattagcaac ttccgcaatc
    17581 atattgatag tgcggtcaag aaaatatctg tcgtgcgaaa caagaataaa ggtaccatca
    17641 taatcctgca gatatttttc aagccattgt atggaaggta aatcgaggtg attggttggc
    17701 tcatcaagca ttaacagact tggtttcttc aacagcattc gtgcaagcat tacgcgcata
    17761 cgccatcctc cggaaaattc tttcaacgga cgttgcaaat caatagtact gaaacctaaa
    17821 ccttctaaaa ttgattccgc ccgatgtgtg atgttgtaac catcaagaga ttcgaattca
    17881 ctctgaagaa aaaacagttc gttcaatagc tcttcgccat gttctgtttc aagttgtttt
    17941 aaaatttttt caatcttttg atgcagaata ttttctcttt caaaaacttc aagtgccaca
    18001 tcaagaatac ttttgtctga ttcgtatgaa agtaaatcct gatttaaaaa acctacagta
    18061 agatcttttc ttcctgtaat ctcacccccg gttaaagtat attctccatt aataatccgc
    18121 agtaatgttg atttacctgt gccattagcg cctatgagtc ctattttctg attgggttta
    18181 atgtgccaga atgcatcatg ataaagatat cttcctccaa aatcaaaact tatattattt
    18241 aaaactaaca tgctgcaaaa atataaaacc gttttactat tctatctaaa tatctattca
    18301 taattactct taaaaactat tccttaattg ttaaatgttt gatgcaatct tatctgaaaa
    18361 aaatctgata atttaatttg ctgaaaaatt ttaaatcaga cgattatgtc acagtgaaaa
    18421 tgaacctcag caacagtttc agatttctct gcgatcgagt ttttcgaact cactgttatt
    18481 gctgatatat tcaggcacta tttctttcat cttcgaaacc aatgccatat tgttttgggt
    18541 cttaaacagg ttggtaaggt tttcaatatc gcttgcaatc tgatcgaatt tatatttccg
    18601 cactttggca attaaaattt taggatgatg tgttgctatg gtgttctctt gtttattcaa
    18661 caactcttca aacaactttt ccccgggtct taaacctgta tattgaattt taatatcctt
    18721 ctcaggttct aatccactca ggcgaatcat cttctttgca agctcagcaa tcttaacggg
    18781 tttacccata tcgaatataa aaatttcgcc accctttccc attttgcaag cttcaagcac
    18841 aagctgacaa gcctcaggaa tggtcataaa aaaacgggta atattttcat ctgtcactgt
    18901 tacaggcatg ccgttttcta tctgcttctt aaatcgtgga atcactgatc ctgctgaacc
    18961 aagaacattg ccgaatcgtg ttgtgataaa ttgtgtttcg gaaatctcgc tgagcgattg
    19021 aatataaatt tctgcaacac gttttgatgc acccataacg tttgtgggat taacagcctt
    19081 atctgtcgaa atcatcacaa acttctgcac tccaaattca acagacaggt ctgcaagaat
    19141 tttagtacct ttaacgttgg tcagaattgc ttctgaagga ttcacttcca taacaggcac
    19201 atgcttatag gctgctgcat gaaatacaat ttcaggtttg aaataatcaa acaccctgcg
    19261 catacggtca atgttgcgga tgtcagcaat cacactttcg caattgcctg catgtttaat
    19321 ttcctgtatg gaaagttcaa gctcataaag tggcgactct gcaatgtcga gaagaaccac
    19381 ttttgcgggg ttgtataaaa ttacctgtcg cgcaatttca gagccaatag atcctgctgc
    19441 tccggtgatg agaatagttt ttccgtttag ctgattacgg atttccttta catcaagatt
    19501 tataggatca cgctcaagca agtcttcaat gtttacttcc ttgatttgag aaaaactcag
    19561 tttgccgttt atccagtttc cggcacgtgg agcacgctga acttttgtat cgtactttac
    19621 acaaatttca attaattcat tgatgcgctt agtgtcaaca tccttatctg caataatcag
    19681 gctcgatatt ttattggctc gaagcaagtc ttccaaatcg gtctttgtat aaaaaaccgg
    19741 tatgccttca agctttcttt tacggagttt tggcttatca tcaataaatc caagaacctt
    19801 aaaatttatt cctgcatctt gttccaaagc atgtttcaca accattcctt cttcacctgc
    19861 acccataata agcacatcgg ttttattacc cttctttctt gtccactcca cgtgtgcact
    19921 ttttacaatc acacgcattg atgccaatgc aagcatactc agaaaaaagt ctatcatcag
    19981 aatagagtaa ggcgtaaaat aacgtccata aattctgaat gaaataaggt taaagatgca
    20041 taacaaaact gttcccgaca gcaaggttaa aaatattcgt tgcgaatctt tgtagcttgt
    20101 atagcggatt ataccggtat gcgttcttga tattaaaaaa ctaaccaatc ttacacttaa
    20161 aactgttgca aacgctgtgg gcaagagttg aatttctaca tcagggatag taaaattaaa
    20221 acgcaaatca taagccagat agaatgacaa agtgcataat atcaaatcta ttactaagac
    20281 tacccaacgt ggtgctgtac gctcaaaaag aaacattcct caggctttac aaaatttaaa
    20341 attgaacgca aaaatagtac ttttattagc gctttatcac tgtaaatgct ttgaatatca
    20401 atatttcata aatattaaca agtttaacgg tctgtagcct ttgatgtcta tattttcaat
    20461 acagaagtat taaccacctt gcattagtaa tccactgata attaaagcag taacttaaaa
    20521 taatttttac tgcggaattg ttatagaata agaaaaagtg ctatatttgc agcccgtttg
    20581 ataaaaactt tattataaaa tgaaaagcga aatacatcca aagaactacc gcatggtagt
    20641 atttaaagat atcagttgcg attatgcgtt cctaacgcgt tcatgtgttg atacaaaaga
    20701 caccattaag tgggaagatg gaaatgaata tccattggta aaacttgaga tttcgaatat
    20761 gtcgcatcct tactacaccg gtaagatgaa actgattgac actgcaggac gtgtggataa
    20821 attccgtaac cgttatgcta aatcaactgc aaaaaaagca gctaaaacaa ctgagaaata
    20881 attttagaaa ttttcagaaa agatcctctg cagcttgcag gggatttttt ttgacataac
    20941 cccggaaatt taccgcattt tataattacg taattttgta aagtgattac cgacaaaaaa
    21001 tcagatgcct ggataagtgt agttgacgaa tccatcaata ccgatgtttc gcgcataatg
    21061 aatctattta ttcaatgcag cagcaacttt ttttctgcag tgttgataga tacagaccgg
    21121 aacaagtttg tagcactctt cgactatcgt tttccatatc ctctgagcaa tgatgatctg
    21181 acagaaaaaa ttcttcagat ttttgatcag aacaaaccac ttttagataa tcactatgat
    21241 agtgttgtgc tttcattttg cggatttaac aatacccttg ttcctgatgt gttttttgat
    21301 gccgaccgtt tagatgagtt ttactatgcc aaccatcagc acgaatctac agagacaaca
    21361 aataaattac tgttcgataa cctaagacgt atttctgcaa ggaatatcta ttctgtgcct
    21421 gaatcgctgt ttgacttttt taattcatgg tattcaaacc taagcgtttt tcatggtgca
    21481 actccattga ttgaaggtgt gcttacctta cataaaaata cctcagaaaa gacagtaacc
    21541 gtcaatattc aaaacaccta ttttgagatg gttgtcaatc agggtggaaa tttgctttac
    21601 tacaacaagt ttaactttca gtcaacagaa gactttgttt attttcttgt tttcgtttat
    21661 gagcagcttg aaatgaatcc cgagtcgcag gttttgaata ttgcaggaac agcagaaaaa
    21721 aattcagcat taatagtgtt ggcgtcaaaa tatatccgcc acattcagtt tgtgcaacga
    21781 ccttcgtttg ctttttacag ctatggattt gatgaagtag cagcacatta tcatttcagc
    21841 ctttataatc agtttttgtg cgtataatca gcggacggtt gcgggggaaa aaaattacag
    21901 ctcctgataa tttaccggtt cgtcctacta ctgattttgc caaaacagga ttgtttaata
    21961 ttctttctaa ccatttgaat ttttccacag taactgcact cgacttgttt acaggaacag
    22021 gaaatatttc ttacgaactg atttcgcgtg gtgtaaaaaa tgtaacagca gtagacgcag
    22081 acaaacgatg cattgcattt gttcaaaaaa tatttaatga tatacatgct gagaatgcag
    22141 aggctataca atctgatgca ttggaatttt tagagcaatg tagcagaact tttgacttaa
    22201 tttttgcaga cccgccattt aattcaaatc cgatacagga tattgtagaa attgtattca
    22261 gaaaaaaatt attaacctca caaggacttt taattataga acacagcagc gaaatgcagg
    22321 ctcccaaatc cgacaattta cttgaaacac gaacttatgg tgctgttgca ttcagtatat
    22381 tcgtagctga aaaataacat caatgatgaa aactgcagta ttccccggat cgtttgaccc
    22441 cattcacaac gggcacttgg agattgtaca acgtatgttg cccttattcg ataaaataat
    22501 tattgccata ggaataaatc ccgacaaaaa atatttgttc agcttagaac aacgtttaaa
    22561 ctggctggag caggttttta aaaacgaatc aaaaatcaac attacaagct atcatgggct
    22621 tacagcagaa ttctgcaaac agcagcaggc acaatacatg attcgcggac tccgcaacag
    22681 cagcgatttt gaatttgaaa aagccattgc acagatgaat aaaaaacttg gtggcataga
    22741 aacagtattt gttatatcaa gtcctgaaaa tgcaccactt tcatcaacca tattgcgcga
    22801 cattattatc aatggcggca gtgtggaagg tttggttcct gtgaagattg aaaatcagaa
    22861 aaaataaatt atttttttag taggcactat tgcctctgca agtcaaaaca aggttttcta
    22921 tattatttta gatgcataag cggtttgaca aatacactta gcgaactttg aatgcgactg
    22981 gaaattgtaa gagttaaagg gcgcgagcgg aaatgatgct tgcggctata aagggggaat
    23041 tcatgtcttt tgagtttagt aaatctgttt aattgatgtt acgaaacacg tttgcctaaa
    23101 taatctttac cattgtataa gaacacacgt ggaattttct ttgctttctc acgatatagc
    23161 tcatcccaag gtctatcttc catttgttca ataattttaa ttgcttgagg aaaatactca
    23221 agagtttcgg ttacatcacc ataagcgaaa atgaacgctt gtccgattgc attaaaaata
    23281 ccatacacat gaatgctgtt tttgtttttt acttctgccg ataaaacaat gttatcatat
    23341 tggtcaatca cttcaactgc tgttgaatca taatttcttt tgtagaaatt attcttgtta
    23401 accgtccatt cattgtcatc aatttcacaa accattttgc cggccttatc aaatatttta
    23461 gttgaaataa aaagtttacc acctttcaat ttgataataa ctggaacagt atttccaact
    23521 tgcaaacatt ttggagtttc aacacccgct tcaaattgac ttttcttaaa tgcacatgtc
    23581 atacccccaa ttttcaattc aaccatttct tcaggtagtt gtgctgcttg tgaatgcggt
    23641 gctggtttgc tttctggtgc tacaatttca ccttgcattg cgttttcacg ctgcgctgct
    23701 tctttctcct ttcgggcttt ctctttcgta aaataatctt tggcataatt tgcaattaaa
    23761 attaacccac cacaaacaaa aattgtaatt gtcgcccact taatatattt ttcttccatt
    23821 gtcgttatga attagcacta aagtaaagtt tattagaatt ccaaaagttg aaagttagca
    23881 ggatgccatt tgcgcataac acagcctttc cgataactat cggaattaaa actaatcagt
    23941 agctcaaaag taaaaagaaa aatgattcaa ggaggcaata gcaaagaatc aggttgtatt
    24001 aaaattaatt gtttgtgtta aatcacgctg tcaggggcgg tgcgacctga cccttaggtt
    24061 tgtgaaatca aaaaaatgta aatgcacatt atatcattaa taattatttg gtatttttat
    24121 ccgtgattta ataacaatag acatagcttt attgaaaatg gttttttcac cgtctgacgt
    24181 tatcggtact aaatgacggc agtgagaact ttcagctttc ggaattctaa tgagtatttc
    24241 tttcgagtga actcaaacaa ttgtatcact aatgaacaaa ataatataaa acagactttg
    24301 aaaaacgtaa ccactttaat ctgtactttc ttgctattgt caagcagtgc ttttgctcaa
    24361 acaagctatc ctaaaggcgg atacaaatcc tttgaagaat ttcaaaataa gcaaccttct
    24421 attccatttg tatttgaaat aaccagaaga acaaaaggtg acattaaaat gaatggtggt
    24481 gttgattata aagttacaag tgattcgatt agtaaatcca ccatcaagag ggatatttat
    24541 gcaatttcaa gtggtgacac attatacatg aattgtttta cgcacaaagt tcaattttgg
    24601 tatgcaaaag ttttagtaga aggaaaatat attgcatttg aaggcggcat tccaatggac
    24661 aaaaatagtg gtgttgttgc agcaggtgtt gcatttgggc ctttgggagg agggattgcc
    24721 ggtgcgcatg cagcaatgct gcgatattta tatataatgg aagctgctgt tggaaaaatt
    24781 aaacagtttg acagtaaata tttaattact ctgcttgagt cttttcctga tttaaaaaat
    24841 caatacttaa atgagccaaa acaggcagat gaagaaactt tgttgaaata tcttagactg
    24901 attaattcta attgacaact ttactgccgt taacagtaat aatttaacct gacaaggcgc
    24961 atagcccctg taagtcaaat caaggcttta ttctatttta gatgcataac cggtttgaca
    25021 aatacactta gcgaactttg aatgcgactg gaagtcgtaa gagttaaagg gcacgagcgg
    25081 aaaggacgct cgcggctaca aaggggtaaa ttttactttt attgatttta cgagtgtaaa
    25141 aataataaaa aattactctt gttttgtatt cgagtgtaaa aattactctt aattgaaatt
    25201 tcagtctata ttttcggaat tgctctgatg gtcttatccc ttattatagc cagtggttct
    25261 tctcgtcttt ctttgcaaac cattgtccgt attgagtttc tgtgtcattg agtgtcgggt
    25321 tttccagttg agtattttat tttacgcttt cactacttct ttccctgcct tgccggcagg
    25381 cgattcaaaa aatactttgg caaattattt tatagtcttt caataatgtc ttaaaagtta
    25441 tgagcggaaa tgacgcttgc gcctataagg tttaatggca cgaacgtgaa tgacgctcgc
    25501 gcctacaagg cttgctttct ctattaaaag ctttgggttt ggggtggggc tgcgcggctt
    25561 ttaattgtga aagcaaatat gatggcgatt ggaatatagt cataagaaac atttactgct
    25621 ttacaagttt tcgcgtacat gaattgttgc aattagtaat tcttaaaaaa taaataccat
    25681 ttggttgagc tgataaatca attttgcatg aattattttt tgtagtagta caggaaatat
    25741 ttttagacaa aatattgtac ac
//
LOCUS       DAOOOI010000025        25062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_361_length_25062_cov_15.060943, whole genome shotgun sequence.
ACCESSION   DAOOOI010000025 DAOOOI010000000
VERSION     DAOOOI010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 25062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25062
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_361_length_25062_cov_15.060943"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1821)
                     /locus_tag="PKX59_05040"
     CDS             complement(112..1821)
                     /locus_tag="PKX59_05040"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOZ90266.1"
                     /translation="MKAFHFLKYNESTSIDKLIAQSAPETVLCFDLEDSIQDCLDSGK
                     TTVLKKIFREYFRSMLVACKTDDGKLNIGVRINSTEGMEYNLDLLSLSGTGQISTVFL
                     PKVESAQQILKLQNDLQENNISFDEIIPVIETKVGLKNLSSILKSDLKKVKRFAFGHC
                     DYNNDCGNFPFFHQDSREYWTWITKIFKELKLYGYTIVNSPCLQLDNEVYFLNMLNIL
                     VSICGSDCAQVTLTKTQTEVCVSYSVIKPDAELPLMGNRLDFKVPAVYIESFIKEFEN
                     KYHEKGFVISNGRVLISPQEYVAALNYRKKANLPEINFTFLGGCFPVQGNMPFENRFH
                     QLLKDKVENCRNVKFNINIIRYERFKNCFNKIETYNKSHPIDVLVVSIRPEPVLRLIK
                     LYYKFKKNPGGRIERSLNIPLLNKIDAESYDLLSASTRFNPVENTANSKMHSALVDLN
                     YFFGMLIGNYSYALEKYKELLKDIIKFTNNRNIQLIVLGPPERTNALFEKLITLRLEK
                     DIKKYLEYIPECFVSGRSQNYKGEILFDSLGVYATKAYHLMIAQALYEKILPNIDDIN
                     IRK"
     gene            complement(2262..3641)
                     /locus_tag="PKX59_05045"
     CDS             complement(2262..3641)
                     /locus_tag="PKX59_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HOZ90267.1"
                     /translation="MKRNIVVVIIFLLLFGAQSCRKAEVLSESEWNEWLAGGKQTLFD
                     ESAGAFSSVFPDISAHNAMIHEMGDKAFEATFVSAPAPLNPGLGPIFNNVSCASCHIA
                     DGRGKPPLNSSEPLSAMLIRVSIPGVASNGGPLAVPGFGVQLQQRSINGVAKEADVII
                     NYSEQTFSFPDGETYSLRTPTYQLANSYIPLPAGVMLSPRMAPPVFGLGLLEAVDESE
                     ILKFADENDANGDGISGKPNYVWNVLAGTRTLGRFGWKANNPTVLQQTAGAYNEDMGI
                     TSYVFPAESSKGQTQYDNRNDDPEIPDSVLRAVSFYVQTLAVPVRRSADDATVLQGKK
                     LFNDAKCGSCHRPAMQTGVRVDFPEVSNQRIFPYTDLLLHDMGEGLADHRPDFDANGN
                     EWRTAPLWGIGLTQKVNGHNFYLHDGRARSLQEAILWHGGEAESAKNAFKNMSKSDRQ
                     ALIKFLQSL"
     gene            complement(3644..4693)
                     /locus_tag="PKX59_05050"
     CDS             complement(3644..4693)
                     /locus_tag="PKX59_05050"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HOZ90268.1"
                     /translation="MQFRFILFSLASVLVLSSCKKDKSNSDDYSSTQILNDFSVNVAQ
                     ATYNDLASKTEQLHQAITNFTQNQTQANLDNCKSLWKQSRQAWEQSEGFLFGPVATDD
                     IDPRIDTWPVDFNSLDSVLNNQSVYDEAYVNSLEDALKGFHPVEYLLWGVDGNKTIAQ
                     FTSRQLDYLEALAANLKTLTQQVASSWTTGNYHNEFANAGNGSQVFTTQHAAFMEMVS
                     AMAGICEEVADGKMEDPLVSQNAMLEESPFAKNSIIDFTNNIKSVENVYLGKYTSQGQ
                     SLESFVRANNLSLDGSIKTKISAAITALGNITVPFGQAIFTQQTQVINAQNAIRDLKS
                     VLENDLANLVSQKIQ"
     gene            complement(4701..5123)
                     /locus_tag="PKX59_05055"
     CDS             complement(4701..5123)
                     /locus_tag="PKX59_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HOZ90269.1"
                     /translation="MKRRNFIKNTCMSCGMMAAGGSLLSLLSSCKSTSIFKAEAVNHQ
                     LTVPLSAMNDKKILIVRNASSEYDILLVKKPDNHFTALEMKCTHRANPLNASDGGLYC
                     TLHGSRFDLDGNVVAEPATQPLKKYPVVVNNSLIQISI"
     gene            complement(5126..6313)
                     /locus_tag="PKX59_05060"
     CDS             complement(5126..6313)
                     /locus_tag="PKX59_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90270.1"
                     /translation="MKNYITVILFLFPFLSQAQTGEDSLHTNAANEKQTYISGYGEAM
                     VSYDSRYRTAQANFTRAVVFVGHKFSKNISFLSETELADARVEGGKAGGEIALEQAYL
                     RFQLNRKMHLQAGLFIPRIGIINENHLPTTYHGNQRPFTETFLIPSTWRELGVALYGS
                     SDAITGLNYSVALVNGLDASGFSNGTGIRGGRFEGGNATASNIALTGSVQYVMKNFTF
                     QLSAYYGGSAGLTKREADSLQLNSGMFGTPVGLGEVNVQYRKDRLTAKVLFAAITIPD
                     ADKINRAYANNTAELSVGGYAEVAYRILKKLKTDAFVRYENFDLNSSIPKNGIKNDIL
                     KQQYIIAGFSCFPTPGVVVKLDYVYKQTGDLNPALNLNPFPNAPAYYTTNSFINLGIG
                     YSF"
     gene            complement(6368..7939)
                     /locus_tag="PKX59_05065"
     CDS             complement(6368..7939)
                     /locus_tag="PKX59_05065"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOZ90271.1"
                     /translation="MMTGRLQSFSKPLFFAGVLLFAVGLPLSMFLMSVSQALIIVAWL
                     LNGDLKEKLKTALSNKIVLLLVVFFLWHLLGLLYTTDFHYGFKDVRVKLPLLLIPIIF
                     SSMPVFSDKQFKTVALVFIASVLVSTLISMCIYVGIIPVEFHNVRDISIFISHIRLSL
                     LICIAVYLSFWLLRKIKFSYGLLLVIIWFVFFLSLIESVTGLGILAIVLLIYAIKTFL
                     KNKSVVSRGIIAGSVVMVVTILTFTIKSAWHDVTFFNDAELRNLAKHTANGAPYTHDT
                     LNRESENGHPVWINICEPELRTAWNKRSKINYDDKNLNGDEIKLTIFRFLSSKGLTKD
                     AHAVEQLSNTEINAIEHGVANAHDLIDSNIHNRLRATLWELNRYRNGGNASGHSLTMR
                     FEFWKTALQIIKQHPLTGVGTGDVPDAFVKKYDETNSLLDAKFRLRSHNQFLAVAVAL
                     GIPALLLFIVMLFYPVFQFQYSGSFLYLTFWITAFLSMLTEDTLETQAGVSFFIFFNC
                     LFLFLKDKELDNNFI"
     gene            complement(7952..9628)
                     /locus_tag="PKX59_05070"
     CDS             complement(7952..9628)
                     /locus_tag="PKX59_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOZ90272.1"
                     /translation="MRKRIRLYVAVILVGISAFSVYSFSDDYFEISRNLDIFATLFRE
                     LNIYYVDETNPGDLMKKGIDDMLASLDPYTNYIPESEIEDYRFMTTGQYGGIGSLIGS
                     RNNEVIITDPYEGFPAQKADLRAGDVIVELDGKPIKGKKNDEVSKLLKGQPKTSVKIT
                     IRREGEPNLIDKNVVREEIKINSVSYSGIIDNDLGYIRLTGFTENAGQEVKNALQALE
                     AKTKLKGLIFDLRGNPGGLLNEAVNIVNIFVNKGVEVVSTKGKAKEWDKTYHALNNPV
                     DTEIPVAVLVNSSSASASEIVSGSLQDLDRGIIIGQRTFGKGLVQTTRPLSYGAQLKV
                     TTAKYYIPSGRCIQALDYSHRNDDGSVGKIPDSLITQFKTKSGRTVYDGGGVMPDFVT
                     DVRQLSPISQSLLLKYLLFDYATMYRNTHPSIAPAKEFHLSDAEYAEFVKWISAKDYD
                     YETKSEKILDDFKTTAEKEKYFSAAAAEFEVLKKKISHDKNADLQKFKDEITELLENE
                     IVSRYYYQSGQIEASFKNDLEIKKATEALKNKDVYSSIMKSSMASVREKH"
     gene            complement(9680..9901)
                     /gene="yidD"
                     /locus_tag="PKX59_05075"
     CDS             complement(9680..9901)
                     /gene="yidD"
                     /locus_tag="PKX59_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOZ90273.1"
                     /translation="MIKLLNRILIAIVKFYKMVISPLLPMSCRYYPTCSEFCMEALKK
                     YGPLKGGFLSLKRILSCNPFGGHGHHPVP"
     gene            complement(9852..10292)
                     /gene="rnpA"
                     /locus_tag="PKX59_05080"
     CDS             complement(9852..10292)
                     /gene="rnpA"
                     /locus_tag="PKX59_05080"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOZ90274.1"
                     /translation="MNEGTLRNTFSKAERLCKRKDFELIFSQGAAFNRFPFRVHWLKL
                     KENTSTVPVKIAISVPKRKIRSAVSRNRIKRLIRESYRLNKQILSNELKKNEQVIHIM
                     FVYNGNINATFTEIQSKIILILQRLKELNDQTAEQDSDSHSKVL"
     gene            10391..11008
                     /locus_tag="PKX59_05085"
     CDS             10391..11008
                     /locus_tag="PKX59_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90275.1"
                     /translation="MLKSGLALCLLFILSLGNINAKETIFKEQRIGFGYYPSFHGSQN
                     FWLNYSVNKRSRKTIYSIRGMYNFRSEKAQEVSNYAIGPCVSVKLLSFFKERLEIRPT
                     LYIPYVNFKYEKSTYPGSLYATQYNGAALLPGLNIQTELTDRIGVRYGANAGIYYAKT
                     REPIFEQKNGVTTYDVYTDKNMYWIMLIDFSITIKITKNYLPHQR"
     gene            complement(11019..11774)
                     /locus_tag="PKX59_05090"
     CDS             complement(11019..11774)
                     /locus_tag="PKX59_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ90276.1"
                     /translation="MNPIITVNNLVKHYDSFHAVKGISFEVYEGEIFGLLGPNGAGKT
                     TTLEIMETLRAKTSGDITIKGNKVGTDDDAIKKIIGVQLQAAGYYPSLSLLELIDLFA
                     GLYSQDVNAMELLQKVGLTDKAKSKYKELSGGQKQRFSIATTLINKPAVIFLDEPTTG
                     LDPQARRNLWDLIRQIKNEGTTVVITTHYMDEAEELCSRVAIIENGKIIALDTPDQLI
                     DDLVAKGFERKKEVKKANLEDVFLNLTGQEWRE"
     gene            11849..12433
                     /locus_tag="PKX59_05095"
     CDS             11849..12433
                     /locus_tag="PKX59_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOZ90277.1"
                     /translation="MLKVDDILVSDQVITEKFICDLSKCKGACCVEGESGAPLEEDEL
                     KALEKVYPKVKPYLTDEGIKAIEAQGFHLKDDDDEWVTPLMWPGGPCAYTVFENGIAL
                     CGIEKAYRDKKIKFYKPISCHLYPIRVLKTKSGDALNYHEWEVCSPACKLGKKEKVRV
                     YEFLKTALTRKYGEAWYNKLIKAAQEYLQTLTKK"
     gene            12488..>13239
                     /locus_tag="PKX59_05100"
     CDS             12488..>13239
                     /locus_tag="PKX59_05100"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ90278.1"
                     /translation="MYLPKHVISWLFFLIIIQSATAQTTISGTVTDQKNNPVAGANII
                     IRDSYDGASSDASGKYQFNANDSGDVIIVCSLIGFSPSDKKIKLQGNTLEINFKLKET
                     SNELNTVTITAGAFEASDEKKMVILRPLDIVTTAGSQGDIYGALQTLPGTQPTQDQEG
                     LFVRGGDAAEAKTLIDGIEVANPYFSSVPDVPQRSRFSPFMFKGTNFSTGGYSAQYGQ
                     AMSSVLALESEDLPPRSVGNIGIMSVGGGGGY"
     assembly_gap    13240..13249
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13250..13439)
                     /locus_tag="PKX59_05105"
     CDS             complement(<13250..13439)
                     /locus_tag="PKX59_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90279.1"
                     /translation="MWRRIPVAGLFDDSKKGCKGKKICKFPGGSFFPNYVQALHHTPK
                     QHDYDFRLLDMVMKVNKTQ"
     gene            13458..14738
                     /locus_tag="PKX59_05110"
     CDS             13458..14738
                     /locus_tag="PKX59_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ90280.1"
                     /translation="MFRHKTSETGIIKAFINYQYSDLSLNFDNLEDVTGKSKNHFGLN
                     NYNIFSTLSYKEVLNKWTVQAAGSYSKDINRIYARPQHLLFSNQLWQGRAVVSRSISE
                     LSSIRGGVEVKYNDFSNLYNNFRVDSTEMFTAVFLEGDAYITRKLVARAGLRVENSYL
                     LRASNLAPRISVAYKLGKFDQLNIAYGDYYQTPDKNYLIFTNLDHFEKATHYILNYQY
                     VNEKRTFRVEAYYKKYNHLVQTTNMASDVYATAFATGDDGKGYAQGIDFFWRDKQTFK
                     YVDYWISYSFLDTKRKYLYYPFEVQPTFASKHVVSVVMKRWFPKLRSSIGFTYVYAAG
                     RPYYNPNKTNENNFLSDRTNDYNNLSLTWSYLTSIGRHFTVISAAVNNVLGIESAYSY
                     RYSTDGLRRSAVGPTSKRSFFIGVFISFGEDRTE"
     gene            14762..15400
                     /locus_tag="PKX59_05115"
     CDS             14762..15400
                     /locus_tag="PKX59_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90281.1"
                     /translation="MKKLTFLLLFIAGNMFAQSNSKFEKTMVKQIDLLDKAKAGTDFV
                     NAANAFERIANAETKEWLPLYYQAYALVLSSNDEKDNVKKDAVLDKALEIANKADKLS
                     PDNSEIVTLQSWITSMKIGIDPATRGQKLGMEAGMLTAHAIELNPENPRPYFLQGTGL
                     LFTPEQFGGGKAKALPVLEKAIEKFNAFKPANAMMPNWGKEFAQKMLDECKK"
     gene            complement(15532..16167)
                     /locus_tag="PKX59_05120"
     CDS             complement(15532..16167)
                     /locus_tag="PKX59_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ90282.1"
                     /translation="MQIKKFTFNPFEENTYIVYDETAQCAIIDPGCYMPEEKEELLSF
                     IAEQKLNPVLLLNTHCHVDHIFGNKLIYDTFGLKPQFHQLELRLLEAAGEYSKQWGIF
                     LEPSPTAEKYLEEGDIISFGNISLEILLTPGHSPGSLCFYDKQEKIIIGGDVLFQMSI
                     GRYDLPGGDEQTLLNSIRTKLFTLPADVKVLPGHGPETTIGFEKMYNPFLV"
     gene            16762..17358
                     /locus_tag="PKX59_05125"
     CDS             16762..17358
                     /locus_tag="PKX59_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOZ90283.1"
                     /translation="MEYIKHLSKDKKLASVIKQHGTVELKKRKDLFLSLCGSIISQQL
                     SVKVADVIKQRFYDLFDGKPTPEKILQATTETLRSIGLSNSKASYIKNIAAFALENSL
                     QLNKVNKMSNDEFIEYITQIKGVGRWTAEMIMMFTLGREDVFSVDDYGIQQSVIGLYK
                     IKISDKKKLRLRILAIAEQWAPYRTYACMYLWRWRDNG"
     gene            17447..18175
                     /locus_tag="PKX59_05130"
     CDS             17447..18175
                     /locus_tag="PKX59_05130"
                     /inference="COORDINATES: protein motif:HMM:NF018473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HOZ90284.1"
                     /translation="MKRYLFILITLFSSVALAQQSGNGFAVVELFTSQGCGSCPAAET
                     VLKKEMDDAIKNNKPVYFLSFHVDYWNKYGWKDPYSSIKYSRRQNNYVSATGSNEVFT
                     PQVFVNGNTGFVGSDSKRLNSEIEKGLKSVAKQKLTVTVLQTLNDTLHLNYTASKADA
                     NANIIVAVVQKSVTTKITKGENIGRTLVQDNVVRLYEVYPVATKGTLLLKTNKLALNE
                     NFQLIAFIQNKQSKKISAATALTL"
     gene            complement(18190..19368)
                     /locus_tag="PKX59_05135"
     CDS             complement(18190..19368)
                     /locus_tag="PKX59_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ90285.1"
                     /translation="MSKDRFQAPDYFLMDELLTDEHKLIRDSVREWVKKEVSPIIEDA
                     CQKCEFPKHLIKGLAEVGAFGPYIPAEYGGGGLDQISYGLIMQELERGDSGIRSTASV
                     QSSLVMYPIYTYGSEAQRKKYLPKLASGEWMGCFGLTEPDHGSNPGGMVTNIKDKGDH
                     YLLNGAKMWISNAPFAQVAVVWAKDEEGVIRGLIVERGMKGFTTPETHGKWSLRASAT
                     GELVFDNVEVPKENLLPNVKGLKGPLGCLNSARYGIAWGALGAAMDCYDSALRYAKER
                     KQFGRPIGGFQLQQKKLAEMLTEITKGQLLVWRLGKLKNENRANPAQISMAKRNNVHT
                     ALWCAREARQIHGGMGITGEYPIMRHMMNLESVITYEGTHDIHLLITGMDITGENAFT
                     "
     gene            complement(19460..23122)
                     /gene="metH"
                     /locus_tag="PKX59_05140"
     CDS             complement(19460..23122)
                     /gene="metH"
                     /locus_tag="PKX59_05140"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOZ90286.1"
                     /translation="MKNIRQELEKRILIIDGAMGTMIQRYKLSEEDYRGERFADWKSD
                     LKGNNDLLSLTKPEIIEAIHLEYLEAGADIIETNTFNAQKISLADYHMESLAYELNFE
                     SAKIAKKAVESFKKKHQSDKKFYVAGAVGPTNRTLSLSPDVNRPGFRAVSWDQMVDAY
                     TEQINGLVDGGADLLLVETIFDTLNAKAALVAIQNVMEQKKVDLPVMISGTITDASGR
                     TLSGQTVEAFLNSVSHVNLLSIGLNCALGAKEMRPYLEELSAKSPFYISCYPNAGLPN
                     QFGEYDETPHQMCVQIDDFLESGFVNIIGGCCGTTPEHIKHIAEHAKNHKPRAIPTIE
                     PYLRLSGLEAVTLRPDSNFMNVGERTNVTGSKKFLRLIKDNKYDEALSVAREQVEGGA
                     QAIDINMDEGMLDAVEAMTTFLNLIAAEPDISKVPVMIDSSKFEVLEAGLKCIQGKGI
                     VNSISLKGGEDQFIYEAKTVKKYGAAVIVMLFDENGQADSTPRRIEIAKRAYDILVNK
                     VNFPAQDVIFDPNIFPVATGLEEHRQNAISYFEASKWIKENLPLAKISGGVSNVSFSF
                     RGNDPIREAIHAVFLYYGIKNGMDMGIVNPSQLIVYDEIEKDLLALVEDVILDRREDA
                     TEKLLAKAEELKLSQTTTDKNVKKGNQSANAWRSEDLQSRITHSLIKGIDEFVDQDME
                     EARQQVSKALEVIEGHLMIGMGVVGDLFGQGKMFLPQVVRSARVMKKAVAYLTPFIEA
                     EKVKGQSKGKIVLATVKGDVHDIGKNIVGVVLACNNYEIVDLGVMVAADKILQAARDH
                     QADIIGVSGLITPSLDEMVHVAKEMEREKFTMPLLIGGATTSKVHTAVKIEPNYSGVV
                     VHVNDASRSVPVVSNLLSAEQKEKFIEDIKAENERTRSYHKAERSRGKFLSLKEAQQN
                     KYELNGAVVTEPKFKGVKVFSNYDLSLLTEYIDWTPFFIAWEMKGSYPKILSDPQRGQ
                     EAQKLFDDAQTMLQRIIKEKWLTANAVFGIFPAANKGDDIEVFDERRENKIATLHTIR
                     QQSERATGQKNIALADFIRPSDYIGAFAVSTGFGIEKWLKKFADDHDDYSSIMLKVLA
                     DRLAEAFAEHLHERVRKEFWAYTTDENLNRQDLIKEKYQGIRPAAGYPAQPDHTEKYT
                     LWKLLDVEKNTGITLTEHLAMFPTAAVSGLYFAHPESHYFAVGKIQRDQVEDYAKRKS
                     MTVEEAEQWLGSILGY"
     gene            complement(23161..23823)
                     /gene="mnmD"
                     /locus_tag="PKX59_05145"
     CDS             complement(23161..23823)
                     /gene="mnmD"
                     /locus_tag="PKX59_05145"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HOZ90287.1"
                     /translation="MDSIEIVKTKEGIDTLINTRINEHYHSLHGAMQESMHVFIRNGL
                     LALNKSLPDIKILEMGFGTGLNAILTYRECYVLQKPIHYTTIESSPLPMAIINKLNYF
                     EYFGKALLPMFQKMHDCNWFENVTFGDFVLHKINADMLELKLDGGYDLVYYDAFSPTH
                     QPELWSFEVLHKVYESCNRGAMLVTYCANGEVKRTLKKVGFAVEALPGPKGKREMIRA
                     CK"
     assembly_gap    23939..23980
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            24338..>25062
                     /locus_tag="PKX59_05150"
     CDS             24338..>25062
                     /locus_tag="PKX59_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90288.1"
                     /translation="MKNRTILFAFFLLLVSIGYSQTCTVGYPPPSDNCATAPAIQLNG
                     VYCGNTCNATANDAAWFKNSNEFCNSVSKQTIENNGFYIFQATASSVNFTICTKTGCT
                     RKNNIIGASGIQALIFNFPTANGLGTCGSGSIDGYYCLKQLSGTDCGSCSNPKGCLSA
                     SVNGLSAGKFYYFMVDGYEGDCCDFSIQFTSNITLPVELISFKGKGENAGNHLFWSTA
                     SEKNNDYFNIESSTDATNWRTIN"
BASE COUNT         7774 a         4896 c         4315 g         8025 t
ORIGIN      
        1 actaatgctt gaatgataga tgggcacaag tccgaagact tgcgccagag aggtttttat
       61 gttgttaata aattacatta agctggcagg tatcaaatgg tgcgcagttg gttatttgcg
      121 aatattgatg tcatcaatat taggcaatat tttttcatat agtgcttgag caatcatcaa
      181 atgatatgct tttgttgcat aaacaccaag gctatcaaac aaaatttcgc ctttataatt
      241 ttgacttctt ccacttacaa aacactcagg aatatattcc agatattttt ttatatcctt
      301 ttccaatctt aatgtaatca gtttttcgaa tagtgcattt gttctttcgg gcggacctaa
      361 tacaatgagt tgtatatttc tattattggt gaattttata atatctttca aaagctcttt
      421 atacttttct aaagcataac tataatttcc gataagcata ccaaagaagt aatttaaatc
      481 aaccaatgcg ctatgcatct tagagtttgc tgtattctcc accggattaa atcttgtaga
      541 tgcagaaagt aaatcataac tttctgcatc aattttattc aataaaggaa tattcaatga
      601 tctttcaatt cttccacctg gatttttctt aaacttgtaa tataacttaa tcaaacgcag
      661 caccggttca ggccttatag aaacaacaag tacatcaatt ggatgcgact tattataagt
      721 ttctatctta ttaaaacaat ttttaaatcg ttcatacctt attatattga tattgaactt
      781 tacatttctg caattctcaa ccttatcttt tagcaattga tggaaacgat tttcaaatgg
      841 catatttccc tgcacaggaa aacaaccgcc aagaaatgtg aaatttattt caggtaagtt
      901 agcctttttg cgatagttta atgcagcaac atactcttgt ggagaaatta atactctgcc
      961 attacttata acaaagcctt tttcatgata cttattttca aattccttaa taaaactttc
     1021 aatataaact gctggcactt tgaaatctaa tctatttccc attaaaggta gctcagcatc
     1081 aggttttatt acggaatatg aaacacaaac ttcggtctgc gtttttgtta aagtaacctg
     1141 tgcacagtct gaaccacata ttgaaactaa aatattaagc atattcaaaa aatacacttc
     1201 attatcaagt tgtaaacatg gagaattcac aatggtataa ccgtacaatt ttaattcttt
     1261 aaaaattttg gttatccatg tccagtactc tcttgagtct tgatgaaaaa aaggaaaatt
     1321 accacaatca ttattataat cacaatgacc aaaagcaaaa cgctttactt ttttaaggtc
     1381 actctttaaa attgatgaaa gatttttcaa accaactttg gtttctataa caggaattat
     1441 ttcatcaaag cttatgttgt tttcctgtaa atcattttgc aatttcaaaa tttgttgcgc
     1501 actctcaact tttggaagaa acactgttga aatttgtcca gttcctgaaa gtgacaaaag
     1561 gtctaaatta tattccatgc cttctgttga atttatgcgt acaccaatat tcaacttacc
     1621 atcatctgtt ttgcatgcaa caagcatact tctgaaatat tctctaaata ttttttttaa
     1681 gactgttgtc tttccggagt ctaaacaatc ctgaatactg tcttcaaggt caaaacataa
     1741 aacagtttcc ggtgccgact gcgcaatcaa tttgtctatt gatgtacttt cattatactt
     1801 taaaaaatga aatgctttca tgatcagtcg ttgctggaat aatattagaa attattcctt
     1861 ccacaaactt aataaaaagg tcataaagga aattaaacta tgacattaaa gtatattcct
     1921 tcttgttgga taaattaaac taacactaaa ctacgcttaa cctttcttgt ttttttgcaa
     1981 taaccggaaa tgataaataa tagaaataat acctatgata attgatgcaa tgcctgcgtt
     2041 tttggaagca tcagtaaaaa ctctaaaata agatagaatg cctactatta aaaaaccgaa
     2101 acctgcaatc aaagaaagat acttattgta aaatatcata actcaatctt ataaatacag
     2161 catcaaaagt aaatcgaaac aaaacacttt tgatttattt taaaaattcc tgagcatcca
     2221 tcatcatttc atcacagtca ggaaaattct gaaagaatga attataatga ctgcaaaaat
     2281 tttatcaatg cttgtctgtc gcttttgctc atatttttaa aagcattctt tgccgattct
     2341 gcttcaccgc catgccataa aattgcttcc tgcagcgaac gtgctctgcc atcgtgcaaa
     2401 taaaaattat gaccgtttac cttctgtgtt aaacctatac cccataacgg tgcagtacgc
     2461 cattcattac cattggcatc aaaatcaggt cggtggtctg caagaccttc gcccatgtca
     2521 tgcaacaaca aatctgtgta aggaaaaatt ctttgattac ttacttccgg aaaatcaaca
     2581 cgaacaccgg tttgcatagc aggtcggtgg caagagccac acttggcatc attaaatagt
     2641 tttttgcctt gcaacactgt ggcatcatca gcacttcttc ttacaggcac agctaatgtt
     2701 tgcacataaa atgaaactgc acgcaaaacg ctatcaggaa tttcagggtc atcgtttctg
     2761 ttatcgtact gtgtttgccc tttgcttgac tctgccggaa aaacataact tgtaataccc
     2821 atgtcttcat tataggcacc tgctgtttgt tgcaacactg ttggattgtt tgccttccat
     2881 ccaaagcgac ctaaagtgcg tgttcctgca agtacattcc aaacataatt cggcttgccg
     2941 gaaatgccat caccgttggc atcattttca tcagcaaatt ttaatatttc agattcgtca
     3001 actgcttcga gcaaacccaa accaaataca ggaggtgcca ttcgtggtga aagcattact
     3061 cccgcaggta atggtatgta gctgttggct aactgatatg ttggtgtacg caatgaatat
     3121 gtttcaccgt caggaaaaga aaatgtttgt tcagaataat taatgataac atctgcttct
     3181 ttcgcaacac cattaataga tcgttgttgc aactgcacac caaaacccgg cacagctaat
     3241 ggtccaccgt ttgatgccac accgggtatg cttactctga ttaacattgc agagagtggc
     3301 tcactgctat tcaacggagg cttacctctg ccatctgcaa tatgacatga tgcacaggaa
     3361 acattgttga agataggtcc aagtccggga ttcaatggtg caggtgcact gacaaatgtt
     3421 gcttcaaaag ctttgtcacc catttcatga atcattgcat tgtgtgctga aatatcagga
     3481 aacacactgg agaatgctcc cgcagactcg tcaaaaagtg tttgtttacc tcctgcaagc
     3541 cattcattcc actctgattc tgacagcact tcggcctttc tgcaagactg tgctccgaat
     3601 aaaagcagaa aaataattac aacaacaatg ttccgtttca agattattga attttctgac
     3661 taactaaatt tgctaaatca ttttctaaaa cacttttcaa atcacgaata gcattctgtg
     3721 cattgataac ctgtgtttgt tgcgtaaata tggcctgacc aaaaggaact gtaatatttc
     3781 ctaatgcagt tatagcagca ctgattttcg ttttaataga accatctaaa gaaaggttgt
     3841 tggctctgac aaagctctct aaagattgcc cctgagaagt atattttccc agataaacat
     3901 tctctacact ttttatgttg ttcgtaaaat caataattga attttttgca aaaggcgatt
     3961 cttcaagcat agcattttgt gaaactaatg gatcttccat ctttccatct gctacttctt
     4021 cgcaaatacc tgccatagca ctaaccattt ccataaatgc cgcatgctgt gtagtaaaaa
     4081 cctgactgcc atttcctgca tttgcaaact cattatgata gtttcctgta gtccaacttg
     4141 aagctacttg ctgcgttagt gttttcagat ttgctgcaag tgcttctaag taatctaact
     4201 gacgtgaagt aaactgtgca atagttttat ttccatcaac accccacaac aaatactcaa
     4261 caggatgaaa tccttttaaa gcatcttcaa gtgaattgac ataggcttca tcataaacag
     4321 attgattatt caaaactgaa tcgagtgaat taaaatctac aggccaggtg tcaatacgtg
     4381 ggtctatatc gtctgttgca acagggccga ataaaaatcc ttcggactgc tcccatgctt
     4441 ggcggctttg tttccataac gatttacagt tatcaagatt tgcctgtgtc tgattttggg
     4501 taaagtttgt aattgcctga tgcaactgct ctgttttaga ggctaagtca ttataagtag
     4561 cctgtgcaac attaactgaa aagtcattaa gaatttgtgt tgaagaatag tcatcactgt
     4621 tgcttttatc ttttttgcag gaacttaaaa ccaacactga tgctaatgag aataaaataa
     4681 atctgaattg catgattaaa ttaaattgat atttgaatta gtgaattgtt tactactaca
     4741 ggatattttt tgagtggttg tgttgcaggt tcggctacaa catttccgtc aaggtcgaag
     4801 cggctgccat gaagtgtgca gtacaagccg ccatcacttg cattgagtgg gttggcgcga
     4861 tgtgtgcatt tcatctctaa ggcagtaaaa tgattgtcag gtttttttac caataagata
     4921 tcatactctg atgaagcatt acgcacaatt aaaatctttt tatcgttcat tgctgacagt
     4981 ggaactgtca gttgatgatt cacggcttct gctttgaata ttgacgtgct cttacaagaa
     5041 gaaagcagcg acagtaaact tcctcctgct gccatcatgc cacaactcat acatgtgttc
     5101 tttataaaat tcctgcgttt catttttaaa aactatagcc tatgccaaga tttataaaac
     5161 tgtttgttgt atagtatgca ggtgcattcg gaaatggatt caggtttaat gcaggattaa
     5221 gatcaccggt ttgcttatat acgtaatcta attttaccac tacaccgggt gtaggaaaac
     5281 aagaaaatcc tgcaatgata tattgctgtt tcagaatatc gttctttatt ccattttttg
     5341 gaatactgct gttaagatca aaattttcat aacggacaaa agcatctgtt tttagttttt
     5401 tgagaatgcg ataagcaact tctgcataac caccaacaga taattcggct gtgttgttgg
     5461 cataggcacg attaatttta tcagcatcag gaatagtgat tgcagcaaaa agcacttttg
     5521 ctgtaagtct gtctttgcga tactgaacat ttacttcgcc aagccccaca ggggtgccaa
     5581 acatgcctga gtttaactga aggctatcag cttctctctt tgtcaggcct gcgcttccac
     5641 cataataagc tgaaagctga aaagtgaaat ttttcattac atattgcact gatcctgtca
     5701 aagcaatatt agaagcagtt gcattgccac cctcaaacct acctccgcga ataccagttc
     5761 cattgctgaa gcccgatgca tcaagaccgt tgacaagtgc aacagaataa tttaatcctg
     5821 ttatagcatc tgaactacca tacaaggcaa cacccaactc gcgccatgtt gatggtatta
     5881 gaaaagtttc tgtaaagggg cgttgattac catgataagt tgttggtaaa tggttctcat
     5941 taataattcc aatgcgcgga ataaataatc cggcctgcaa atgcattttt ctgtttagct
     6001 gaaaacgcag ataagcttgc tccaatgcta tttctccacc tgccttgcca ccttcaacac
     6061 gtgcatcggc caattctgtt tctgaaagaa aagaaatatt cttgctgaat ttatggccta
     6121 caaaaactac agctctggta aaatttgcct gagcagttct gtagcgtgaa tcataactta
     6181 ccatcgcttc gccatagcct gatatgtagg tctgcttttc attagctgca ttggtatgta
     6241 atgaatcctc acctgtctgt gcctgagaaa ggaatggaaa tagaaacaga atgactgtta
     6301 tgtagttttt cattgaatga aaattatcgg ctgcgaaagt aatgagaaaa tactatttag
     6361 aatgtttcta aataaaatta ttatctaact ctttgtcttt cagaaacaaa aacaggcaat
     6421 taaaaaaaat gaaaaaacta actccagctt gtgtttcaag tgtatcttct gttaacattg
     6481 acaaaaaagc cgtaatccag aaagtcagat ataagaaaga ccctgaatac tgaaattgaa
     6541 aaacaggata aaaaagcatg acgataaaaa gtaaaagagc gggaataccg agtgcaacag
     6601 caacagcaag aaactgatta tgcgaacgca aacggaattt agcatccaac aatgaattgg
     6661 tttcatcata cttttttaca aaagcatcag gcacatcacc cgtgccaaca cctgttaacg
     6721 gatgttgttt gatgatttgc aaggctgtct tccaaaattc aaaacgcatg gttaatgaat
     6781 gtccgctggc attgcctcca tttctatatc ggttcaactc ccaaagtgtt gcccttaatc
     6841 tgttgtggat attagaatcg attaaatcat gtgcattggc tacaccatgt tctattgcat
     6901 taatctcagt attactgagc tgttcaactg catgtgcatc ttttgttaat cctttggaag
     6961 ataaaaaccg gaatattgtt aatttaatct catcaccatt cagattttta tcatcataat
     7021 ttatcttgct tctcttgttc catgctgtcc gcaattccgg ctcacaaatg ttaatccaca
     7081 ccggatgtcc gttttccgat tcacgattca gagtgtcatg tgtatatgga gcaccatttg
     7141 ctgtatgttt tgctaaattt ctcaactcag catcattaaa aaatgtcaca tcatgccatg
     7201 cgcttttgat agtgaatgta agtattgtaa caaccattac aactgaacct gctataattc
     7261 ctctgctaac tacagattta ttctttagaa atgttttgat agcataaatc aataacacta
     7321 ttgccagtat gcccaaaccg gtgacagact caatcaatga aagaaaaaac acaaaccaga
     7381 taattacaag cagcaatcca taactaaact tgatttttct taacagccaa aatgacaaat
     7441 aaacagcaat acaaataagg agtgacaatc tgatgtgtga aataaaaatt gaaatatctc
     7501 tgacattgtg aaactcaaca ggtattattc ctacataaat acacatgctt attaatgtgg
     7561 acactaaaac ggatgcaata aaaactaatg ctactgtttt aaattgttta tcagaaaaaa
     7621 caggcattga tgaaaaaata attgggatta aaagcaaagg aagtttgaca cgcacatcct
     7681 taaagccata atgaaaatcg gtagtataaa gtaatcccaa cagatgccat aaaaagaaaa
     7741 caaccagcag taaaacaatt ttattagata atgctgtctt taatttctct ttcaaatctc
     7801 cattcaaaag ccatgcaaca atgatgaggg cttgagaaac actcatcaaa aacatggaga
     7861 gtggcaatcc aacagcaaac agtaaaacac cggcaaaaaa aagaggttta ctaaaacttt
     7921 gtagccggcc tgtcatcatt aatgcagtat tctagtgctt ctctctaacc gaagccatag
     7981 agcttttcat gattgaacta tagacatctt tattcttcaa tgcttctgta gccttcttaa
     8041 tttccaaatc gttcttaaaa gatgcttcaa tctgtcctga ctgataataa tagcgtgaca
     8101 caatttcatt ctccaacaac tctgttatct catctttaaa cttttgtaga tctgcattct
     8161 tatcatgcga tatttttttc ttcagcactt caaactctgc tgctgctgca ctaaaatatt
     8221 tttctttttc tgcagttgtt ttaaaatcat caagaatttt ttcgctcttg gtttcataat
     8281 cataatcctt tgcagaaatc cattttacaa actcagcata ttcagcatcg gagagatgaa
     8341 attcttttgc aggtgcaatt gatggatgtg tattacgata cattgttgca taatcaaaca
     8401 ataaatattt cagtaataaa ctttgcgaaa taggactcag ttgtctgacg tcagtaacaa
     8461 aatcaggcat aacaccaccg ccatcataaa cagtacggcc agacttcgtt ttaaattgtg
     8521 tgatgagtga atcaggaatt tttcctacgc tgccatcgtc attgcgatgt gaatagtcga
     8581 gtgcctgaat gcaacgacca ctgggaatat agtattttgc tgtggtaact ttaagttgtg
     8641 caccataact taacggtctg gtagtttgca caagtccttt accgaaagta cgctggccaa
     8701 taatgatgcc tctgtctaaa tcctgcaaac ttcctgaaac aatttctgat gcagatgcgc
     8761 tgctgctgtt tactaaaact gctacaggaa tttctgtgtc aacaggattg ttcaatgcat
     8821 gataggtttt atcccattct tttgctttgc cttttgtgct aacgacttca acacccttat
     8881 tgacaaaaat atttacaatg ttaaccgctt cgttaagtaa tccacccggg tttccgcgta
     8941 aatcaaaaat caacccttta agttttgtct ttgcttccaa agcttgcaat gcatttttta
     9001 cttcctgtcc tgcattttct gtaaatccgg taaggcggat ataacctaaa tcgttatcaa
     9061 taattccact gtaagaaaca ctgtttattt taatctcttc acgtactaca tttttatcaa
     9121 tcaggtttgg ttcaccttca cgtctgatag ttattttaac tgaagttttt ggctgacctt
     9181 taagcagctt actcacttca tcattctttt ttcctttaat gggcttaccg tctagttcaa
     9241 caattacatc acctgcacgc aggtcggctt tttgagcagg aaatccttca taaggatcgg
     9301 taatgattac ttcgttattg cgcgaaccga taagtgagcc aatgccacca tactgtcctg
     9361 tagtcatgaa acgatagtct tcaatttcac tttcgggaat ataattggtg tatggatcaa
     9421 gtgaggcaag catatcatca atgccttttt tcatcaggtc accgggattt gtttcatcaa
     9481 cataataaat gttcaactca cgaaacaagg tagcgaaaat gtcgaggttg cgcgatattt
     9541 caaaataatc gtcactgaaa ctataaacag aaaaagcaga tatgccaacc agaataacgg
     9601 caacatacaa tctgattctt ttacgcattt tttgtagcat cttataaact atttaatcaa
     9661 ttaatatttt taaaaatcat taaggcaccg gatgatgtcc atggccacca aatggattgc
     9721 aggataatat tctttttagt gataaaaaac cacctttcaa cggtccatac tttttcagcg
     9781 cttccataca aaactcgctg catgtaggat aataacgaca tgacataggc aacaatggcg
     9841 atatgaccat tttataaaac tttactatgg ctatcagaat cctgttcagc agtttgatca
     9901 ttcaattctt ttaaacgttg taaaattagg attattttag attgtatctc tgtgaaggta
     9961 gcgttaatat ttccattata aacaaacata atatgtatga cctgctcatt ttttttcagt
    10021 tcgttgctca atatctgctt attaagtctg taactttcac gaataagcct tttaatacgg
    10081 ttgcgggaaa cggcacttct tatttttcgt ttcggtacac ttatggcaat tttaacagga
    10141 actgttgatg tgttttcttt taacttcagc caatgcactc tgaacggaaa acggttaaac
    10201 gctgcaccct gactaaaaat cagttcaaaa tctttccttt tacaaagcct ttctgcctta
    10261 gaaaatgtgt ttcgcaaagt gccctcattc attgttttgc aaaagtacaa acattgtatc
    10321 ggacagataa ttatttttca ggattatatt gtttttggtt ctaactttat aatttggcga
    10381 aaatttattt atgcttaaat ccgggctggc actatgttta ttgttcatac tatcgttggg
    10441 caacatcaat gctaaggaaa ctatttttaa agaacaacgc ataggttttg gatattatcc
    10501 gtcttttcat ggatctcaaa atttttggtt gaactacagt gtaaataagc gcagccgcaa
    10561 aaccatttat tcaatccgag gcatgtataa tttccgctcc gaaaaagcac aagaagtaag
    10621 taattatgct attggcccct gtgtttctgt aaagctacta tcgtttttta aggaaagatt
    10681 agaaattcgt cccacactgt atatccctta tgtaaatttt aaatacgaaa aatcaaccta
    10741 ccctggaagt ttatatgcaa cacagtataa tggtgcagcc ttattgcccg gtttaaatat
    10801 tcagaccgaa cttaccgaca gaattggtgt gcgctatggt gctaatgcag gaatctatta
    10861 tgctaaaaca cgtgaaccca tttttgaaca gaaaaacgga gtaacaactt atgatgttta
    10921 caccgataaa aatatgtatt ggataatgct gattgatttt agtattacaa ttaaaatcac
    10981 aaaaaactat ttaccgcatc agcgataaaa aacaatcatt attctcgcca ttcctgacct
    11041 gtaagattca gaaaaacgtc ttcaaggttt gcttttttaa cttctttttt tcgctcaaaa
    11101 ccttttgcta ctaaatcgtc tatcaactgg tcaggagtat cgagtgcaat aatttttcca
    11161 ttttcgataa tagctactct gctgcacaac tcttctgcct cgtccatata gtgtgtagta
    11221 ataactacag tagtgccttc attttttatc tgtcgaatca agtcccaaag atttcttctt
    11281 gcctgtgggt ctaatcctgt agtaggttca tccagaaaaa tcactgcagg tttgttgatt
    11341 aatgttgttg caatagaaaa tcgctgtttc tgcccacctg acaattcttt gtattttgat
    11401 tttgccttat ccgttaagcc tactttctga agtaattcca ttgcattaac atcctgactg
    11461 tacaatccgg caaataggtc aatcaactca agtaatgaaa gacttggata atagcctgca
    11521 gcctgcaact gtacgccaat tatttttttt atcgcatcat catcagtacc aactttattt
    11581 cctttaatag ttatgtcacc tgatgttttg gcacgaaggg tttccattat ttcaagcgtt
    11641 gtcgttttac cggcaccatt aggtccgagt aaaccaaaaa tctcaccttc atacacttca
    11701 aaagatattc ctttaacagc gtgaaagcta tcgtaatgtt tgactaaatt atttaccgtt
    11761 ataattggat tcatgatgtg caaagttata acatttgact tccttcctca aaatgccatg
    11821 aagttttacc tttgccaaaa ataaaccggt gttaaaagtt gacgacatac tggtatcgga
    11881 ccaggttatt acagagaaat ttatttgcga tctttctaaa tgtaaaggag catgttgtgt
    11941 agaaggtgaa tcgggcgcgc cattagaaga agatgagttg aaagcattgg aaaaagttta
    12001 tcccaaagtt aaaccttatt taaccgatga gggaataaaa gctattgaag cgcagggatt
    12061 tcatctgaaa gatgatgatg atgaatgggt gacacctttg atgtggcccg gtggcccatg
    12121 cgcttacacc gtttttgaaa atggtattgc actatgcgga attgaaaaag catatcgaga
    12181 taaaaaaata aaattttata agccaatcag ttgtcatctt tatcctatac gtgttttaaa
    12241 aacaaaatcg ggtgatgcat taaactatca tgaatgggaa gtttgcagtc ctgcctgtaa
    12301 gttgggaaag aaagaaaaag ttcgggttta tgaatttctg aaaactgcac tgaccagaaa
    12361 atatggcgaa gcatggtaca acaaattaat aaaggctgca caggagtatt tacagacact
    12421 gacaaaaaaa taatatcttg acaaaacaat taaatctttt acctttgttt aaccataacc
    12481 gaatcaaatg tatttaccta agcatgtaat cagttggttg ttttttttaa ttatcataca
    12541 atcagcaacc gcacaaacta caatttcagg aacagttacc gatcagaaaa ataatcctgt
    12601 tgcaggagcc aacatcatta tccgcgacag ttatgatggg gccagcagcg atgcttcggg
    12661 gaaatatcaa ttcaatgcaa atgattccgg tgatgtgata atagtttgca gcctgattgg
    12721 ttttagtcct tcagataaaa aaataaaact acaaggtaac acacttgaaa taaatttcaa
    12781 actcaaagaa acaagcaatg agctgaatac agtaactata acagcaggag cctttgaggc
    12841 aagtgatgaa aagaaaatgg tcattttacg accattggat attgttacaa cagcaggttc
    12901 gcagggtgac atttacggag cgctgcaaac acttcccggc acacagccta cacaagatca
    12961 ggaaggactt tttgtgcgtg gtggcgatgc agcagaagcc aaaacactta ttgatggaat
    13021 agaggttgca aacccatatt tctcaagtgt tcctgatgtt ccgcaacgca gcaggttttc
    13081 tccttttatg tttaaaggca caaacttcag caccggaggt tattctgcgc aatatggtca
    13141 ggccatgtca tcggtgcttg cattagaaag cgaagacctt cctccacgct ctgtcggaaa
    13201 cattggcatc atgtctgttg gtggtggtgg tggttacatn nnnnnnnnnt ttgagttttg
    13261 ttcaccttca tcaccatatc cagcaaacgg aaatcatagt cgtgttgttt tggtgtatgg
    13321 tgcagtgcct gcacatagtt tggaaaaaaa ctgccgccgg ggaatttgca aattttttta
    13381 cccttgcacc cttttttgct atcatcaaac aaacccgcga ctgggattct cctccacaaa
    13441 ctttcagcag ttctgcagtg ttcagacata agacatctga aacaggaatt attaaagcat
    13501 ttatcaatta tcagtacagc gacctctcgc tcaacttcga caatctggaa gacgttaccg
    13561 gaaaaagtaa aaatcatttt ggtctcaaca actacaacat cttttctacg ctttcatata
    13621 aagaagtgct caataaatgg acagtgcagg cagcaggatc ttacagcaag gatattaatc
    13681 gtatttatgc aaggccacaa catctgcttt tcagtaatca actgtggcaa gggcgtgcag
    13741 tagtgtcccg ttcaataagt gaattgagct ccatacgtgg tggcgtagaa gtaaaatata
    13801 atgacttcag taacctctat aataatttca gagtggatag tacagaaatg tttacggcag
    13861 tttttcttga aggcgatgcc tacatcacaa gaaaattagt agctcgtgcc ggactaagag
    13921 ttgaaaattc atacctgctt cgtgcaagta atcttgcacc acgcatcagc gtggcttata
    13981 aattaggaaa gtttgatcag ttgaatattg cctatggaga ttattatcaa acacccgata
    14041 aaaattatct catctttacc aacctcgatc attttgaaaa agcaacacat tacattttga
    14101 actatcagta tgtaaatgaa aaaagaacct ttcgtgttga agcatattat aaaaaataca
    14161 accacttagt gcagactaca aatatggcat cggatgtata tgctacagca tttgctaccg
    14221 gtgatgatgg aaaaggctat gcacaaggaa tagatttttt ctggcgcgat aaacaaactt
    14281 ttaaatatgt ggattattgg atttcctatt cattcctcga caccaaaaga aaatatttat
    14341 actatccgtt tgaagtgcag cccacctttg cttcaaagca tgtggtgagt gtagtgatga
    14401 aacgctggtt tccaaaattg cgaagcagca ttggtttcac ctatgtgtat gcagcaggaa
    14461 gaccttatta taatcctaat aaaaccaatg aaaataattt tctttccgat cgcactaacg
    14521 attataacaa cctgagttta acctggagct atttaacatc cattggcaga cactttacag
    14581 taatttctgc tgccgtaaat aatgtgttgg gtattgaaag tgcatacagc tatcgttaca
    14641 gcactgacgg gctaagaaga agtgctgttg gcccgacttc caaacgctcc ttttttattg
    14701 gtgtatttat cagtttcgga gaagacagaa cagaataaaa aaattcaaat caaattataa
    14761 aatgaaaaaa ctcacattcc ttttactctt tattgcaggc aacatgtttg cacaaagcaa
    14821 cagcaagttt gaaaaaacta tggttaaaca aattgatttg cttgataaag ccaaagcagg
    14881 tactgacttt gtgaatgccg ccaatgcatt tgagcgcatt gcaaatgcag aaaccaagga
    14941 gtggctgcct ttatactatc aggcttatgc tttagtactt tcttcaaatg atgaaaaaga
    15001 caatgtaaag aaagatgccg ttttagacaa agcgcttgaa attgccaata aagcagataa
    15061 actctcacct gataattcag agattgttac actgcaatcg tggataacct caatgaaaat
    15121 tggtattgac cctgcaacac gcggacaaaa attagggatg gaagcaggca tgcttacagc
    15181 acatgccatt gaactgaatc cggaaaatcc acgtccctat tttctgcaag gtacaggatt
    15241 gttgtttact cctgaacaat ttggtggtgg caaagccaaa gcattacctg ttcttgaaaa
    15301 ggcaatagag aaatttaatg catttaaacc tgccaacgct atgatgccaa actggggaaa
    15361 agagtttgct cagaaaatgc ttgatgagtg taagaaatag gctttagttt ggaagggttg
    15421 gaaagtttgg aaggttggaa agtttggaag gttggagggt tggaaaagtt gtaatgttta
    15481 aagcagagaa tgcgcgagcg aaacgctcgg gcattccttg cttaatgcac cttaaactaa
    15541 aaaaggattg tacatttttt caaaacctat tgttgtttcg gggccatgac ccggaagcac
    15601 ttttacatct gcaggcagtg taaacagttt tgttctgatg ctattcaaca atgtttgctc
    15661 atcaccaccg ggtaaatcat aacggccgat gctcatctga aacaatacat cacctccaat
    15721 aataattttc tcttgtttat cataaaaaca taaacttccg gggctgtgac cgggagttaa
    15781 taatatttct aatgaaatat taccaaatga aatgatgtct ccttcttcca aatacttttc
    15841 tgccgttggt gatggctcca gaaatattcc ccattgctta ctgtattctc ctgctgcttc
    15901 aagcaatctt aactctaact gatgaaactg tggttttaat ccgaaagtat catagataag
    15961 tttgtttccg aaaatatgat ctacatggca atgtgtattt agcaacaaaa cagggtttag
    16021 tttttgttct gcaataaaac tgagtaattc ttccttctct tcaggcatat agcatcccgg
    16081 atctataatg gcacactgtg ctgtttcatc ataaacaata tatgtgtttt cctcaaaagg
    16141 attaaatgta aatttcttta tctgcatgtt attagccaat gaaagtgcaa attaaagaat
    16201 attaacgtga gtttagaagg tttagactgt ttggaaagtt ttgaatgttt agaatgttat
    16261 taatgcagct aatttttaag tttctgattt gtgacttctt gaaaagtttg aaggtttaga
    16321 atgttttgaa agttacaatg ttttgaaagt tacaatgttt tgaaagttat tgatttcgct
    16381 aatttttaca tttctgattt gtgacttctt gaaagtttga aggtttggaa agtttagaat
    16441 gttacaatgt tttgaaagtt attgatttcg ttatttttta agtttctgat ttgtgacttc
    16501 ttgaaagctt gaaagtttga aggtttggaa agtttagaat gttacaatgt tttgaaagtt
    16561 actgatgcag ctaattttta agtttctgat ttgtgacttc ttgaaagttt gaaggtttaa
    16621 aatgtttgga aagttggaat gcaggatttt aattggaatt ttacttagat gatttgttga
    16681 ttagatactt agataatgtt aaatgtttgt tgtaataaag attattttat caagtgtaat
    16741 acattagcct aaattttaaa tatggaatac atcaaacatc tttctaaaga taaaaaatta
    16801 gcatcggtaa ttaagcaaca tggaactgta gaacttaaaa aaagaaaaga cttgtttctg
    16861 agtttatgcg gttctattat atcacaacag ttgtctgtaa aagttgctga cgtaattaaa
    16921 cagcgttttt atgatttatt tgatggaaag cctactcccg aaaaaatact tcaggcaaca
    16981 acagaaactt taagaagtat tggattgagt aattctaaag caagctatat taaaaacatt
    17041 gcagcttttg cactggaaaa cagtttgcag ttgaacaagg taaataaaat gagtaatgac
    17101 gaatttattg aatacattac tcaaataaaa ggtgttggcc gttggactgc agaaatgatt
    17161 atgatgttta ccctaggtcg cgaagatgtt ttttctgttg acgattatgg tattcagcaa
    17221 tccgttatcg gactttataa gattaaaata tccgataaga aaaagttgcg cctgcgaatt
    17281 ttagctatcg ctgagcaatg ggcaccttac agaacttatg catgcatgta tctgtggcga
    17341 tggcgagata atggttgaaa atcaaatctt atttgctcat aacttcatgg ataccacata
    17401 taaactaata taattttcta catttttgcg ttattgcttt gtattcatga aaagatattt
    17461 attcattctt ataacattgt tttcatcagt tgctttagca caacaatccg gcaacggttt
    17521 tgctgtggta gagcttttta cctcacaggg ttgtggcagt tgtccggcag cagaaacagt
    17581 tctgaaaaaa gaaatggatg atgccattaa gaataataag cctgtttatt ttttaagctt
    17641 tcatgttgac tactggaata aatatggatg gaaagatcca tacagcagca ttaagtattc
    17701 aagacgacag aataattatg ttagtgcaac aggcagcaat gaagtgttta cgccacaagt
    17761 gtttgttaac ggcaacacag gctttgtagg ctctgacagc aaacgtctga attcagaaat
    17821 tgaaaaaggt cttaaatcag ttgctaaaca aaaactgacg gttacagttt tacaaaccct
    17881 aaatgacact ttacatctta actatactgc aagtaaagcc gatgccaatg ctaatattat
    17941 tgtagccgta gtacaaaaaa gtgttacaac aaaaattaca aaaggtgaaa acataggaag
    18001 aacacttgtt caagacaacg tagtgagact ttacgaagta tatcctgttg ccacaaaagg
    18061 aacactgctt ttaaaaacca ataaacttgc actcaatgag aattttcaac tcattgcatt
    18121 catacaaaac aaacaaagca aaaaaatttc tgcagctact gcgctaacgc tttagttact
    18181 ttttcctgat caggtaaaag cattctctcc tgttatatcc attcctgtaa tgagcaaatg
    18241 aatgtcatgc gtaccttcat aagtaattac cgactcaagg ttcatcatgt ggcgcatgat
    18301 aggatattct cctgtgatgc ccatgccgcc atgtatctgt cttgcttcgc gggcacacca
    18361 caatgcagtg tgaacgttgt tacgtttagc catacttatt tgtgcaggat tggctctgtt
    18421 ttcattcttt aattttccaa gccgccatac caacaactga cctttggtaa tttctgtcag
    18481 catttcggca agttttttct gctgaagctg aaagcctcca attggtcttc cgaattgttt
    18541 acgctctttc gcatagcgta aagcgctgtc ataacaatcc atggctgcac ctaaagctcc
    18601 ccaagcaatg ccataacgtg cactgttcaa acatcctaat ggtcctttta aaccttttac
    18661 gttaggcaat aaattttctt taggcacttc tacattatca aaaaccaact cacctgttgc
    18721 actagcacgt aagctccatt tgccatgtgt ttcaggagtt gtaaaacctt tcatgccacg
    18781 ttcaactatc aagcctctga tgacaccttc ttcgtctttt gcccaaacta ctgcaacctg
    18841 tgcaaacggt gcattgctta tccacatctt tgcaccattc aataaataat gatcgccttt
    18901 atctttaata ttggtaacca taccaccggg atttgaacca tggtcaggct ctgttaaacc
    18961 aaaacatccc atccattctc ctgatgcaag tttgggtaaa tattttttac gctgtgcttc
    19021 gcttccgtag gtataaatag gatacatcac taacgaactc tgtaccgatg cggttgaacg
    19081 gataccactg tcgccacgct caagttcctg cataattaaa ccatagctga tctgatccaa
    19141 tccaccacca ccatactctg ccggaatata agggccgaat gcacctacct ctgccaaacc
    19201 ttttattaaa tgctttggga actcacactt ctgacaggca tcttcaataa tcggagaaac
    19261 ctctttcttt acccactcgc gcacactgtc acgaatcagc ttatgttcgt ccgttaataa
    19321 ttcatccatt aaaaaataat ccggtgcctg aaatctgtct tttgacatac tttaaatttt
    19381 agtctgcaaa aataggattt ttggttgaag gaaaatcagc ttttgaagga attaaatttg
    19441 taagaataat ctcctcaaat taataaccta aaatacttcc cagccattgt tcggcttctt
    19501 caacagtcat tgatttacgt tttgcataat cctcaacttg gtcgcgctga atttttccta
    19561 ctgcaaaata atgcgattcg ggatgtgcaa aatacaatcc actgacggca gcagttggaa
    19621 acatagccaa atgttctgta agcgtaatgc ctgtattttt ttctacatca agtagtttcc
    19681 aaagtgtgta tttctctgta tggtcgggct gtgccggata gcctgcagca ggacgaatgc
    19741 cctgatactt ttctttgatt aaatcctgac gattcagatt ttcatctgtt gtataagccc
    19801 aaaactcttt gcgtacccgc tcatgcaggt gttcggcaaa tgcttcggct aatcggtcgg
    19861 ccaacacttt taacatgatg gatgaataat catcatgatc gtctgcaaat ttctttaacc
    19921 atttttctat tccaaaacct gttgacacag caaaggctcc gatataatct gaaggacgaa
    19981 taaaatctgc caatgcaata ttcttctgtc ctgttgctct ctctgactgc tgacggatag
    20041 tatgaagtgt tgcaatttta ttttctcttc tttcatcaaa cacttcaatg tcatcaccct
    20101 tgttcgctgc aggaaaaatt ccgaatacag catttgctgt aagccatttt tctttaataa
    20161 tacgttgcag cattgtttgt gcatcatcaa aaagtttttg cgcttcctga cctcgttgag
    20221 ggtcgctcaa tattttcgga tagctgcctt tcatttccca tgcaataaaa aatggtgtcc
    20281 agtcaatata ttctgtaagc aatgacaaat catagttgct gaaaactttt actcctttaa
    20341 attttggttc agtaacaacg gctccattca attcatattt attttgttgc gcctctttca
    20401 aagataaaaa ttttcctctt gatcgctctg ccttatgata ggagcgtgtt ctttcattct
    20461 ccgcttttat atcttcaata aatttttctt tttgttctgc ggaaagtaaa ttactcacaa
    20521 caggcacact tcgtgaagca tcattgacat gcacaacaac gcctgaatag tttggctcaa
    20581 tctttacggc agtatgtacc ttacttgtgg tagctccacc aatcagcaat ggcattgtaa
    20641 atttttctcg ctccatttct ttggcaacat gtaccatttc atcaagagaa ggagtaatca
    20701 atccactaac accaataata tctgcttgat gatcgcgtgc agcctgcaga attttatctg
    20761 ctgcaaccat tacacccaaa tcaacaattt cgtaattgtt gcaggcaagt acaacaccaa
    20821 caatattttt tccaatatca tgtacatcgc ctttaactgt tgccagaaca atctttcctt
    20881 ttgactgacc ttttactttt tctgcttcaa taaaaggtgt cagataagcc actgcctttt
    20941 tcatcacacg tgcacttctt actacctgag gcagaaacat ttttccttgt ccgaataagt
    21001 cacctaccac acccatacca atcatcagat gaccttcaat aacctcaagt gctttagata
    21061 cctgctgccg cgcctcttcc atatcctgat ctacaaattc gtcaatacct tttatcaatg
    21121 aatgtgtaat acgtgattgc aggtcttcac tgcgccaggc gttggccgat tgatttcctt
    21181 tctttacatt tttatcagtt gtggtctgcg aaagttttaa ctcttcggct tttgccaaga
    21241 gtttttcagt tgcatcttct ctcctgtcta agataacatc ttcaaccaat gcaagtaaat
    21301 ctttttctat ttcatcataa acgataagtt gcgatgggtt tactatgccc atatccattc
    21361 cattttttat tccataataa agaaacactg catgaatggc ttcgcgaatg gggtcattac
    21421 cacgaaatga aaaagaaaca ttacttacac cacctgaaat ttttgcaaga ggaagatttt
    21481 ctttaatcca tttacttgct tcaaaataac ttattgcatt ctgacgatgt tcttcaagtc
    21541 ctgtggcaac aggaaaaatg ttcgggtcga aaattacatc ctgagcaggg aagttaactt
    21601 tgttaactaa aatatcataa gcacgttttg ctatttcaat tctacgtggt gtactatcgg
    21661 cttgtccgtt ttcatcaaag agcataacga taactgcagc accatatttt tttactgtct
    21721 ttgcttcata gataaattga tcttcgccac cttttaatga gatggagttt acaattcctt
    21781 ttccctgaat acattttaat ccggcttcga gcacttcaaa ctttgaagag tcaatcatca
    21841 ccggcacttt tgaaatatca ggctctgcag caatcaggtt cagaaatgtt gtcatggctt
    21901 caacggcatc aagcataccc tcgtccatgt taatgtcaat tgcctgcgca cccccttcaa
    21961 cctgctcgcg tgctacactc aatgcttcat cgtacttatt atcttttatc aggcgaagaa
    22021 attttttaga gcctgtaaca ttggtgcgtt cacctacatt cataaaattt gaatcaggcc
    22081 tgagtgttac tgcttctaat ccgctcaatc gcagataagg ttctatggtt ggtatagctc
    22141 ttggcttatg attctttgca tgctctgcaa tgtgtttgat gtgttcgggc gtggtgccgc
    22201 aacaaccacc aataatattt acaaatccac tttctaaaaa atcatctatc tgcacacaca
    22261 tctgatgcgg tgtttcatcg tactcaccaa actgattggg cagccccgca ttgggatagc
    22321 atgaaatata aaatggtgat ttcgcactaa gctcttccag ataagggcgc atttcttttg
    22381 cacctaatgc acaattgagg cctatgctta atagattaac atgcgagaca gagttcagaa
    22441 atgcctccac cgtttgtccg cttaatgtac gtccgctggc atctgtaatt gttccggaaa
    22501 tcatgacagg caaatcaacc tttttttgtt ccattacatt ctgaatggct actaatgctg
    22561 ctttggcatt gagtgtatca aaaattgttt caactaaaag taaatctgca ccaccatcaa
    22621 ccaagccatt tatctgctcc gtataagcat ctaccatctg atcccagcta accgctcgga
    22681 aacccggcct gtttacatcg ggcgagagtg acaaagttct gtttgttgga ccaacagcac
    22741 cagcaacata aaacttttta tctgactgat gtttcttttt gaatgattca accgccttct
    22801 tagcaatttt agccgactca aaatttaact cataagccaa tgactccatg tggtaatcgg
    22861 ctaatgaaat tttctgcgca ttaaaagtgt tagtttcaat aatatcagct cctgcttcga
    22921 gatattccaa gtgaatggct tcaataattt cgggcttggt gagtgagagc aaatcgttat
    22981 tcccctttaa gtcacttttc caatctgcaa acctttcacc tcgataatct tcctcactta
    23041 atttatatcg ctgaatcata gtgcccatgg caccatctat aattaatatt cttttttcta
    23101 actcctgtct aatatttttc atttcactaa agtgttgcaa caattctaat taatgttttt
    23161 ctatttacaa gctctgatca tttcacgttt cccttttggt ccgggcaatg cttcaacagc
    23221 aaacccaact ttttttaatg ttcgctttac ttcaccatta gcacaataag ttaccagcat
    23281 agctcctcta ttgcatgact cataaacctt atgaagcact tcaaaagacc ataactccgg
    23341 ctggtgtgtt ggtgaaaatg catcgtaata gacaaggtca taaccaccat ctaatttcaa
    23401 ctccagcata tcagcattaa ttttatgcaa aacaaaatcg ccaaaagtta cattctcgaa
    23461 ccagttgcag tcatgcattt tttgaaacat gggtagcaat gcttttccaa agtattcaaa
    23521 ataatttaac ttgtttatta ttgccattgg caagggcgat gactcaatag tggtataatg
    23581 aattggcttc tgcaacacat aacattctct gtatgtgagt atggcattga gtcctgtgcc
    23641 aaatcccatt tcaagaattt taatgtcagg aaggctttta ttcaaggcaa gcaaaccatt
    23701 tctaataaac acatgcatac tttcctgcat ggctccatgc aatgaatgat agtgttcatt
    23761 aatccgggtg ttgattagcg tatcaatacc ttcttttgtt tttacaatct caatggagtc
    23821 catctcctta atttaaatca gatttaaaat aaaaaaaatc ttttccgaaa agccgaaaaa
    23881 gattgaacgt ttttaatctg atttcgactt atctgcttcc cttagggaat gtgaattgnn
    23941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcagatggtt gtcaaggcat
    24001 cgcagggcac ttccctccgc ctttctcgat aagcattttg aaaagaacaa ggtgcaaata
    24061 tacagcatac caatcatttg aacaaaacaa ttgcattgaa tttttacact gtacttgaat
    24121 agttgtacta tattaaacaa caattttcct atgataaaac agcactaatg ttctatggca
    24181 tcaagtgctt tttatcttac acccaccctt tttaaattga taaaaaagct taagttttta
    24241 ggggaattac aaattttcat tttttgacct acatcaaatc aaatcacttt ttaaaattct
    24301 acttttatac agtcagattt accataaaat gtagattatg aaaaaccgaa ccattctatt
    24361 tgcatttttc ctattactgg tatcaatagg ttacagtcaa acctgtactg taggctaccc
    24421 tcctccatct gacaattgcg caacagcacc tgcaattcag cttaatggtg tttattgcgg
    24481 aaatacatgc aatgccacag caaatgatgc agcatggttt aaaaacagta atgaattttg
    24541 taattcagtt agcaaacaaa ccatagaaaa taatggtttc tacatttttc aggcaactgc
    24601 atcatcagta aatttcacaa tatgtactaa gacgggatgt acaagaaaga acaacataat
    24661 tggtgcatca ggaattcagg cattgatttt taatttccct acagcaaatg gattggggac
    24721 atgcggttca ggttctattg atggctatta ttgtttaaag caacttagcg gcacagactg
    24781 tggttcatgt agtaatccaa aaggttgtct cagtgcaagt gtcaatggat tgtctgcagg
    24841 taaattttat tattttatgg ttgatggtta tgaaggtgat tgttgtgatt ttagtattca
    24901 gtttacgtca aacattacat tacctgttga gttaatcagt ttcaagggca agggagaaaa
    24961 tgcaggcaat catttgttct ggagtacagc atcagaaaaa aataatgatt attttaatat
    25021 agaaagcagc accgatgcta caaattggcg cactataaac ag
//
LOCUS       DAOOOI010000026        24935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_364_length_24935_cov_34.389228, whole genome shotgun sequence.
ACCESSION   DAOOOI010000026 DAOOOI010000000
VERSION     DAOOOI010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 24935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24935
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_364_length_24935_cov_34.389228"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..4080
                     /locus_tag="PKX59_05155"
     CDS             280..4080
                     /locus_tag="PKX59_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ90289.1"
                     /translation="MMASTGNKQNTDTAGIDSEVLYNRILFAVIAMCYLLFGIFFSEK
                     VIDVLLPLTWMALVPLALLAVSFFLPVAKKYSGEQLSGMFLLLTIHLMFFLFRNKFSG
                     HPEFLLVTVILFSNLHIRNVIYLLLYNVIVLGSLEYILISGTSGSSDPVRLFFIIVIA
                     MVLSFLYQWFSLRRQGMSKDGEKSLENNLEKLPFAAMIFGRDNLGLLYCNREAQNLLG
                     IDSQAEMDIRTVFKLDDFSDTELFAKSKAGNFNGIMAETFQQLKVLLTIRQNEKEELL
                     VFAEREKKTEKESISLPGQYFVTTTDGKIIDLSKEAEDFITVDIGVLQHLFQNFILEN
                     KPLHNFSVLKINEQDPFALQMQTGTLDDIPVYYWTPIKNLQPVESVSSEVILPDDGWS
                     QLVIDSGNELISFSPSFISTVGYDEMELGRIPVVQLIHPADTLRWQQLITECRKKERA
                     NATVRFIHKDGKVHFLKSFFSSNGKDEVRIYAEDITESILLQQELTNARANVSAVIEN
                     TKDLILSTDMNHLITVINHSCQHYLNAVMGKMLNVGDDFRAAMKEEDQMQWSHRHSDV
                     MRGKRVTYNDEISHNGTESYFEFSLQPVVDENGIVTGVSLFGRDVTQRVHFENELVKA
                     KETAETATAAKSQFLATMSHEIRTPLNGIVGMLELLRMTTLNDKQREYVSTLQLSSEN
                     MLSIINDVLDFSKIESDKMELEYEPFELKKVIEETFDLLYYRALGKRLELFYNIENTI
                     PAYVMGDSMRLKQVLVNLVGNAIKFTEKGHILITAQLIPSVSEKLNIKFSVKDTGAGI
                     SEEQRQRLFQSFQQADTSTYRKFGGTGLGLTISAKLVALMGGTIELMSEPGAGSEFYF
                     TVITQPAPIAAARNVRSNLRILRGKKILIFSTNSDFDLHLSDLFNDWHILHQSVTLLE
                     QAKKELSPDANYDALILDAQMQDYLLFAEELKEQIRSSNIPVFAFNARFSGGDIIYGN
                     KLFEAVLPSGVDAIKISSVLTKSFIDSATSFARGDDAVPVFDSQVSLKYPMQILIAED
                     NPINQTLAVTVLEKLGYKPDVVENGKLVIEKVATKQYDLIFMDVQMPEMDGMEATTAL
                     RKLNNNSIIIAMTAFALEDDKKQCLDAGMNDYTTKPIRIEVVQSLLEKWGDVILKSKA
                     ARNDEPLIDKQTIERLKAMAVGDDTSFVKNIFELFLKQMDNLHADMSKFFNEHDADGM
                     YKASHKLKGSALNIGAKRLSEVCRIIEEKGKLGDLTGMNELIQQLAEVISLTKNEIRS
                     LSLA"
     gene            4168..5802
                     /gene="pruA"
                     /locus_tag="PKX59_05160"
     CDS             4168..5802
                     /gene="pruA"
                     /locus_tag="PKX59_05160"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOZ90290.1"
                     /translation="MTNAVYNIPPAYNEPVLNYAPGSPEKMALKAALAEARSKQMDIP
                     MYIDGQEVRTGNTSALRPPHDHQHILGMFHKGEKKHVQLAIDAALRAKKQWAEMPWEQ
                     RAAIFLKAADLLAGPYRYKMNAATMLGQSKNAYQAEIDSACEMIDFLRFNVQYMREIY
                     TQQPPVVGKGVWNRTEQRPLEGFVFALTPFNFTAISGNLPTAPAMMGNTVVWKPANTQ
                     IYAANVVMQLLIEAGLPAGVINLVYVSGPDAGDVIFKHADFGGIHFTGSTEVFKGIWK
                     TIGENMPLYKSYPRIVGETGGKDFIIAHKSADIDVLVTALARGAFEYQGQKCSAASRC
                     YIPKSIFEEVKSKLVKEVKSMKLGPVEDFRNFVNAVIDEKSFDKISAYIENAKKSKSA
                     EIIAGGNFDKSKGYFIEPTIILTSDAKYTTMCEEIFGPVLTMYVYEDDKFEETLHLVD
                     NTSEYALTGAIISQDRYAVDVATRALVNSAGNFYINDKCTGAVVGQQPFGGARGSGTN
                     DKAGAMVNLLRWVSPRTIKETFVPAKDYRYPFMEAE"
     gene            complement(5807..6346)
                     /locus_tag="PKX59_05165"
     CDS             complement(5807..6346)
                     /locus_tag="PKX59_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOZ90291.1"
                     /translation="MKFRTIGLMIFTLLYFGQSAKAQQSENIVEDNLRNYYSQILGYQ
                     VKEVFNTKVFDLIDTWLNTPYKYAGKNENGIDCSGFVNVIIKAVYGSSFGCSSVDMYN
                     NLPHVSLKNLTPGDLVFFKTRGRRISHVGMYLGQDKFVHSSTSSGVIVSDLNEPYYKK
                     RFAKGGRISLDESTTSVSK"
     gene            complement(6461..11689)
                     /locus_tag="PKX59_05170"
     CDS             complement(6461..11689)
                     /locus_tag="PKX59_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90292.1"
                     /translation="MIKSNLISITRSIVAVFLLAVPTCLVAQNYQPKSYYTFEGSSPM
                     KDSMGNFNLDPNYYQSSYQILTNTSGGVGKYMKLDSSSTIIRGGVLAIDSAITFEFIF
                     KPGMNFGTTVFAKRMDGALELRMTYPAINFMTEVKAANGSPIYDDFEIDLNGIGRASY
                     SYYVDDNWHHIVFKYNAKAGIKEVWVDGQLPAGFSKTIAGGSFSQSGNRDYMLNSQSD
                     YYKLQGAIDEIAVYHNSLGGNSIYAHYLNFMAGKHYSFANPTVVAPSPSPVTAGININ
                     DFAPGHPSPSLTPIEQLKNFPTPRYKPGHTLFPNNPFVGYYYLSGYQTYETVYSKAVA
                     NSVTLQTDFAKNFNYTIMAAENTGAYSDYSNSTTFSHAWINLANSYPQYKVSALSYWP
                     QLRPTDAGYKSNSPYATCGCLPDKYYLKNSSGQYLNPNGGTGSTKYLSPAGAPDSLVK
                     DGLTQKFYMQQLLAKLSRPIDYILDNGEVVPFLYYTNVMQSDPSVVSDKNSSGLDWFT
                     YEGRRAATKINAYRDQFMGLSGLSNTKFAYYQLDGQPTWNLKWSEVRTVNTAMNGSHY
                     ATGDIYMRWPSNWRYWSGAWKGWQWVVESRREQLLAGDKYFSPAVSPGWDNDETQNVR
                     PAQWLGFLKAVSMTGAEFFTTGYFVTSQPYQNPKNYIWQLVIPPYAQAITSRYEDLFR
                     NGYLMSGDVPNSTTSPTYMAYSFNTGDPGKLVVVHKHNSQNKYAITGSSQPMSNMAGA
                     APDEAVAQINLDGSRIKFKIRRQGSTYIYDNTNTSAPIFYQLDGWHENTYPYYWSKDF
                     KFEAELNDNSNSNVYLKTTRPAGAAAGDFTDFTTAVGFNSVSDLTYNFQPRSTTDYTY
                     YVWVRARSKNSQTTGMTISLDGSNSKQIGCITDANWQWYKYDVNSGAVISFTSVTSLA
                     DHILKITPTNTNLEIDQIHLSTSSNAIYTSPAPCSGVAVPTISASGPVAFCAGGNVNL
                     IASNGLSYLWSNGSTDPYINVTSAGSYVVTVTTAAGTATSAPVTVTVYAQPNATITAN
                     GSTSFCVGGSVTLTASSGSTYLWSSGATTQSITAIGGGNYNVIVTNSNGCSAMSASTA
                     VTVKPVPTATISPSGPTTFAAGGSVTLTSSSAGTGGSYKWSPGGMTTQAVTVSTTGNY
                     VVTVTNNSGCSATSSPLSVTVTSTAPTPKITANGPTTFCQGGSVVLTCSAANSYLWSN
                     GATTQSITVTTAGSYTCTATTAGGSATAAAVNVIVTTPTQATITASGSTSFCSGGSVT
                     LTASTGTSYMWSTGATSNAITTSSPGTYTVTITNNGCQSTTNKVVTVYALPTATITAN
                     GSTNLQQGGSVQLTASSAGSGGSYLWSTGATTQSITVSTAGNYTVKVTNSNGCSNTSA
                     PMTVTVNSSFQPTITVSGSLSFCSGGNVKLTATKGSSYLWSTGATTRKITVTTAGSYA
                     VTVTQFTTGYVGTSSVVNVNVMPAPSATITASGSTTICQGSSVTLTSSNGANYLWSNG
                     STSQSISASTAGNYIVTVTGSNGCTKSSSATAVVVNNCGSSCPTPGNLHVTNLRPTSA
                     EINWNLTSANNYYIKVENLNTGYVYYSNALSSGVNSITIGASPSTPYRWSVRGLCGST
                     YSSWSAYNQFTTPAARMGEGNSNEGLNITPLTTQMPSELEEQPLRIESDDASVTFFPN
                     PATSFVTFNVTASEKNTATLTLLDLSGRTLNKESYQFNEGLNNGKVDVSNYARGVYMI
                     QLVFDGKTVMKKVILE"
     gene            complement(11957..12694)
                     /locus_tag="PKX59_05175"
     CDS             complement(11957..12694)
                     /locus_tag="PKX59_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90293.1"
                     /translation="MKHIAKLLWLTFFIPLLSSTASAQDTEEETSRWQIGLTAGINIP
                     LSDFADKLDENFKSGYAMLGATSRLRTSYHPNSKLYIAADYVWFYNPSDAKTLLNSLV
                     ETNPSGPRYKTTTYPWKVNSLLLGLGTTLNFNEQTDATLRGMLGFANGKYSKRIYQSF
                     DGVKTLQVTENASSVIDIALNLGFEVKYSFHKNVGVMFAGDYFRTEFNYDDVYLIYSE
                     NGEKRSTGAYSQPVDVFQFSAGIFTKF"
     gene            complement(12891..13337)
                     /locus_tag="PKX59_05180"
     CDS             complement(12891..13337)
                     /locus_tag="PKX59_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOZ90294.1"
                     /translation="MKRIAEIALMSMCVLGVCKSTFVNAQSNIAERKYRVIAYKQGAN
                     EITSMSNETTVTPNMAIYVPNSFTPNGDGINETFGAYSEGVKDFTMQVYDRWGAMIFE
                     SNDISKRWDGMYKGQIAQQGSYVYKIQAKAINGRILTKKGTVNLIM"
     gene            13874..14251
                     /locus_tag="PKX59_05185"
     CDS             13874..14251
                     /locus_tag="PKX59_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90295.1"
                     /translation="MHTIEQLEEMLAEHKEWQGKINFYRAEIDRMKTDLASAVMADRN
                     QYNMPHVEHYQNQFILQRDILDIMRHDFKQHENKIEAHSSNPVTNLAAAHQSEREKLG
                     SFERYFKELRNEFQAFIHKEAFS"
     gene            14405..16207
                     /locus_tag="PKX59_05190"
     CDS             14405..16207
                     /locus_tag="PKX59_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90296.1"
                     /translation="MKKSIICLVLLFHLTVLLFVACRKNDSDDVIPNVQQTSEQIRTT
                     ISGYVLNEMGQALSGVTVSTNGYIATTNDKGIFLLSKVIVDKNRCVISFSKSGFTKRN
                     YVFRPTDSSGQYVKLMLLNNVPNKTVSGVNGGTVSLPNGATIEFQPNSFTTANGTAYT
                     GTVNIYLKHLSPDDTDFSMMMPGGDMLAKDTTGKIVSLYSFGMMMAELTSTNNLPLQL
                     AAGKPATISMPIAPSQILKAPATIPLWYFDETTSLWIEEGGAAKVGSAYTGQVKHFSF
                     WNCDFPGERATIKGRIIDCNGNPMPNVVVTLDNWFDMLTDNNGVYQSWVPANISYVLQ
                     VLLSHNSGVFSSAPVNITPIAPGQTYTVPDISLPCPSSITGTLQNCAGVNVAGYVAVL
                     NLNANLLNLIYTGSGQFSINVPSQIPIVIYVSSLNNFEHYDTLNAISNMGTIINLGSI
                     QLCTYANDTTNLITINGAGFNNQTFIFTNALFMTSDLPSDTSIYSHELELGGILQPYG
                     SCYYYGISLKDTLAGTQPIINSTQVDFQLQTPQVDYAINLLPGGIIELHSNALIPGDY
                     FEGTYSGQADIINLNTVTSTQGTVIGKFRVRKQF"
     gene            complement(16448..16624)
                     /locus_tag="PKX59_05195"
     CDS             complement(16448..16624)
                     /locus_tag="PKX59_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90297.1"
                     /translation="MMNNDPIITYDHTKEENSFRSHYFKKSFFGRKNTMLLFFAEELL
                     ILGFMAYLVYLVFV"
     gene            complement(16846..17472)
                     /locus_tag="PKX59_05200"
     CDS             complement(16846..17472)
                     /locus_tag="PKX59_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ90298.1"
                     /translation="MFTVNLTEAGKKFKQHWIFRKCTAAFSSGDVIGITGKNGSGKST
                     LLQVISGYVSPNEGKIEWHEHDKKIEADSVYRYFSIASPYLMLIEDFTLEEMIRYYFR
                     FKNLRPAFNISSIITESGLEAHRNKLLSEFSSGMKQRTKLLLAIASDTPLLLLDEPCT
                     NLDKQNIAWYQQFLRTHSDGRLIFICSNHIDDELFLCNKWLSVEDFKI"
     gene            complement(17495..18292)
                     /gene="lpxA"
                     /locus_tag="PKX59_05205"
     CDS             complement(17495..18292)
                     /gene="lpxA"
                     /locus_tag="PKX59_05205"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOZ90299.1"
                     /translation="MISPLAVVSPKAKIGNNVTIEPFTMVYDDVVIGDNTWIASNVTI
                     YDGARIGSNCRIFPGAVISAIPQDLKYKGEQTTAEIGDNTTIREFVTMNKGTTDKMKT
                     TLGSNCLVMAYAHIAHDCQLGNHIIMANNVTLAGHVLIDDWAILEGLVAVQQFVHIGA
                     HSFIAGGSLVRKNVPPFTKAAREPLSYIGINNIGLRRRGFSNEDVNHIQDIYRILFVQ
                     NSNVKKALAEIEKEIPESALRSQIVEFIHNSPKGLMRGFTPSDSDDE"
     gene            complement(18289..19683)
                     /locus_tag="PKX59_05210"
     CDS             complement(18289..19683)
                     /locus_tag="PKX59_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOZ90300.1"
                     /translation="MSVKQVTLKEEITVEGPGLHTGVNARLTLKPAPENHGYKFKRTD
                     VNGQPIIDADVDNVVDTSRGTTLSANGVKVSTTEHVLAALVGMEVDNVLIEIDGPEVP
                     ILDGSAFPFVNAIQKAGLREQEATRSYFVITENLTYEEPARNVEMLAVPSPDYRITVM
                     VDYNSPVLGTQHAQMHKITEFKDEISKCRTFVFLHELEMLLKHDLIKGGDLNNAIVIV
                     DREIKQDKLDELATLFKKPKVEIKHKGILNNIELHFQNEPARHKLLDIVGDFALLGVH
                     LKGHILAARPGHAANVAFAKQFKEIIRKDKNRLKAPIIDANKPALFDISRIESFLPHR
                     FPFLMIDKITELTETGVVGVKNVTANEWFFQGHFPGNPVMPGVLLIEAMAQTGGILVL
                     NTVPDPGNYWTYFLKIESARFKNMVKPGDTVVFKLDLISPIRRGLCHMGGQAFVGDKV
                     VMEAEMMAQIVKKS"
     gene            complement(19680..20729)
                     /gene="lpxD"
                     /locus_tag="PKX59_05215"
     CDS             complement(19680..20729)
                     /gene="lpxD"
                     /locus_tag="PKX59_05215"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOZ90301.1"
                     /translation="MKFTAKQIADLLKGTVEGNHDAEVNALSKIEEGKPGTLSFLSNP
                     KYTPYIYDTKASVVIVNKDFVAENAISATLIRVEDAYKSFASLLEIYNQIVNDKKGVE
                     SPSFIDPSATVGDQGYIGAFAYIGKNAVIGKNVKIYPHCYIGDHVIIGDNTTLFPGVR
                     VYSFCQIGMHCNIHAGVIIGADGFGFAPNENNNYKKVPQIGNVVIHDHVEIGAGTTID
                     RATLGSTVIHKGVKLDNLIQIAHNVEIGENTVIAAQTGVAGSTKIGKNCMIGGQVGII
                     GHLSIADGVKIAAQSGIGTSITTPNEIVQGSPAFNISDYKRTYVVFKKLPQLERKINE
                     LEKKTGSLNPAENKS"
     gene            complement(20909..22081)
                     /locus_tag="PKX59_05220"
     CDS             complement(20909..22081)
                     /locus_tag="PKX59_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-aminolevulinic acid dehydratase"
                     /protein_id="HOZ90302.1"
                     /translation="MDASKTLEAATSRLRKYIESHHYKGYDPYDALKSPFFKLPLLNK
                     NKLIRFGAQQLVKRSPLNLRPLLFVPKGLNPVTLGLCIQAYAQLINVNQQEKNILNQN
                     IAKLIDELEKLIPKGFHGACWGYDFDWEARYANIPAYQPTVVATGIITNALFIAWQQT
                     GNQKAFQLCKSAADFVMKDLNRTVDANGHFCFSYSPFDKQVVFNASMKGSRLLAQVYS
                     VTKDESLKKAAADSVAFVMKHQRADGAWIYSTSDAGTWIDNYHTGYVLDCLHEYVLHC
                     DDKTYDENLKAGKKFYINHFFLEGRIPKFYNNNVFPVDCTAAAQSLLTLTRFNEQTLA
                     FKVAEYMLEKMQHQKGYFYFRKYKNYTEKTSFMRWSNAWMFAGLTAVLQSLSANRQ"
     gene            complement(22086..24212)
                     /locus_tag="PKX59_05225"
     CDS             complement(22086..24212)
                     /locus_tag="PKX59_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HOZ90303.1"
                     /translation="MEQLTQNLKDGKMQLLEVPFPVLSSGQVLVRNHYSLISAGTEGK
                     TVKDARLSYIGKARARKEEVKKVIDAAKTFGILNTYKMVMNKLDAPSSLGYSTAGEII
                     AVAPDVTEFRVGDLVACGGNSAVHAEVIAVPVNLCVKLNDKIALQHACFTTLGSIALQ
                     GIRQADLRLGENCVVIGLGLVGQLTVQMLKAGGIKTIGIDIDQRMVDLALHTDIDAAF
                     TRNSEDLESSINNITNGYGTDAVIITAGTDSLDPVDLAGALCRRKGKVVIVGAVPTGF
                     KRPNYFKKELDLRMSCSYGPGRYDTEYEEGGIDYPYDYVRWTENRNMAAFVDLIATGK
                     IHLSNLLTHTFDFKEAPAAYDLIMNKTEPFVGMVLKYDTNKPLQQKVNLSNPFYTKSD
                     VVAGMIGAGSFGQNFLLPALKNTPAKLSGIVTARPNNARNIADKNQFSFASGNAQDIF
                     SNGDINTVFIATRHETHAGYVMQALQAGKNVFVEKPLCLTEEELVQIQHEFTKQNCRV
                     MVGFNRRFAPMIQTIKKTFSSGNPIAINYRINAGVIPADHWVHDLKVGGGRIVGEACH
                     FIDLCMFLAGSKLTTISAVALNDAQKLNDTVCFNLQFENGSTASVSYFSNGNKNINKE
                     YLEVFGSGIVAVLDDFKTLTVFGKSEKKYTGNQDKGHQNEVKAFVDSIVQGKPAPIPF
                     EETVLSTLATFRALQSIASNGERIIL"
     gene            complement(24232..>24935)
                     /locus_tag="PKX59_05230"
     CDS             complement(24232..>24935)
                     /locus_tag="PKX59_05230"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOZ90304.1"
                     /translation="RAGVRPSIEKPLLYEDVNIKGTVNLLEKAKEKNIKQFVFAGSSS
                     VYGINPNTPWSENDFVLKPISPYASTKVSCELTGHTYAHLYGIRFISLRFFTVFGPRQ
                     RPDLAIHKFFKLIRQGTPIPFFGNGETSRDYTFVGDIVNGIIAAMHYDKSNYEIINLG
                     NNRTINLNMLIAAIEKEAGKKALLEKLPDQPGDVPHTFADISKAKELLNYSPATTLEE
                     GLIAFRKWFDQNHST"
BASE COUNT         7588 a         4542 c         4752 g         8053 t
ORIGIN      
        1 gcattagcta attcatctct tcgtttttgg tcatattcaa tagtctctgc agcaacgatt
       61 tcataatgta agccatcatt tgcttctgta atattgacca atgcaaggtc aagcccgtct
      121 aacgatgtgc ccgacatcaa tcccaatact ttcaagcttg cagccatgac ggcaaatgta
      181 agattatggc aaaataatat taagtaattg taacaattca atcaacatta aaaaaatcaa
      241 tttcaaggtt aaccaaatac ctatttttgc gtataagaaa tgatggcttc taccggaaat
      301 aaacagaata cagacaccgc tggcattgat tcggaagtat tgtataaccg catattgttt
      361 gcagttatag caatgtgtta tttgctgttc ggtatttttt ttagtgaaaa agttattgat
      421 gtgctgctac ccctgacatg gatggcactt gttcctttag cattgcttgc agtcagtttt
      481 tttcttcctg ttgcgaaaaa gtattcggga gaacaacttt ccggaatgtt tttattactt
      541 actattcacc tgatgttttt tcttttcagg aataagtttt caggtcatcc tgaattttta
      601 ctagtaactg tcattttatt ttctaacctg catatccgca acgtcattta tcttttgctg
      661 tacaatgtaa ttgtgcttgg ttcattagaa tatattctca tttcgggcac ttcaggaagt
      721 tctgatccgg taagactatt ttttattatt gttattgcaa tggtgttgag ttttctttat
      781 caatggttta gccttcgcag acagggaatg agtaaggacg gtgaaaaatc cttagaaaat
      841 aatcttgaga aacttccttt tgcagcaatg attttcggta gagataattt gggattattg
      901 tattgcaatc gcgaagcaca gaatttactc ggtatagatt cacaagccga aatggatatt
      961 cgtactgttt ttaaattaga tgacttttct gacacagaac tttttgcaaa gtcaaaagca
     1021 ggaaacttta atgggataat ggcagaaact tttcagcaat tgaaagtttt gttaaccatc
     1081 agacaaaatg aaaaggaaga gttgttggta tttgctgaac gcgaaaagaa aacagaaaaa
     1141 gaatccattt cacttcccgg acaatatttt gttacaacaa ccgatgggaa aattattgac
     1201 ctgagtaaag aagcagaaga ttttattacc gttgacattg gtgtacttca acatttgttt
     1261 cagaatttta ttcttgagaa taagccgcta cataattttt cggtgctgaa aattaatgag
     1321 caggatcctt ttgcattgca aatgcagaca ggaacgttag atgatattcc ggtttattat
     1381 tggacaccaa ttaaaaactt gcagcctgtt gagtcagtta gctctgaagt aatattgccc
     1441 gatgacggat ggagtcaatt agtaattgat tcaggaaatg agttaatttc tttcagccct
     1501 tcatttatca gtacagttgg ttatgatgaa atggaacttg ggcgtattcc tgtagttcaa
     1561 ctcattcatc ctgccgatac tttgcgatgg caacaactga ttacagaatg ccgcaaaaaa
     1621 gagagagcaa atgctactgt tagatttata cataaggatg gtaaagttca ttttcttaaa
     1681 tctttcttca gtagtaatgg taaagatgag gtgagaattt atgcagaaga tattactgaa
     1741 tcaatcctgt tgcaacaaga gttaacaaat gcacgtgcca atgtgagcgc tgttattgag
     1801 aataccaagg atttgattct ttcaacagac atgaatcatc taatcacagt aatcaatcat
     1861 tcttgtcagc attatttaaa tgctgtaatg ggaaagatgt tgaatgttgg cgatgatttt
     1921 cgtgctgcaa tgaaagagga agatcaaatg caatggtcgc atcgtcatag tgatgttatg
     1981 aggggaaaaa gggtaactta caatgacgaa attagccata acggaactga gagttatttt
     2041 gaattttcgt tacaaccggt tgttgacgaa aatggcattg ttacaggagt ttctttgttt
     2101 ggtcgtgatg ttacacaaag agttcatttt gaaaatgaat tggtgaaagc taaagaaact
     2161 gctgaaacag ccactgctgc caaatctcag tttcttgcca caatgagtca tgaaatccga
     2221 acaccgctta atggtattgt tgggatgctg gagttattgc gcatgacaac acttaatgat
     2281 aaacaaagag agtatgtgag taccttgcaa ctgagcagtg agaatatgct tagtattatc
     2341 aatgatgttc tcgatttttc taagattgag tcagataaga tggaactcga atatgagcca
     2401 tttgaactga agaaagttat tgaagagacg tttgatttac tctattatcg cgcactgggt
     2461 aagagacttg aattattcta taatattgaa aacaccatac ctgcttatgt catgggtgac
     2521 agtatgcgtt taaaacaagt gctggttaat ttggtaggta atgccataaa gtttacagag
     2581 aaaggacata ttctcattac ggcacaactt attccttctg tatcagaaaa acttaacatc
     2641 aagttttctg taaaagacac cggtgccggc attagcgaag aacaaagaca aagattattt
     2701 cagtcatttc aacaggcaga tacttcaact tacagaaagt ttggtggtac cggacttggt
     2761 ctcactatca gtgccaagtt agttgcttta atgggcggca ctattgagtt gatgagtgaa
     2821 cccggtgccg gttcagaatt ttattttaca gttattacgc agcccgcacc aatagctgct
     2881 gcacgtaatg tccgttctaa cctcaggatt ttaagaggta aaaagatttt gattttttcg
     2941 accaactccg attttgattt gcatctgagc gatttgttta atgactggca tatattacat
     3001 cagtctgtta ccctgctgga acaagcaaag aaagagcttt ctcctgatgc aaactacgat
     3061 gccttaattc ttgatgcaca aatgcaggat tacctacttt ttgcagaaga attaaaagaa
     3121 caaatacgat cttcaaacat tcctgtattt gcattcaatg caagattttc aggaggtgat
     3181 attatttatg gtaataaact ttttgaagct gtactgcctt caggagttga tgcaataaaa
     3241 atttcttcag tattaacaaa gtcgtttatt gacagtgcaa catcatttgc caggggtgat
     3301 gatgctgttc ctgttttcga tagtcaggtg tcattaaaat atccgatgca aattctgata
     3361 gcagaagata atccaatcaa tcaaacgctt gcagttacag tgttggaaaa attgggttac
     3421 aaacctgatg ttgtagagaa tggtaaatta gtgattgaaa aagttgctac caagcaatat
     3481 gatttaattt ttatggatgt gcagatgcct gaaatggatg gtatggaagc aacgacagca
     3541 ttgcgtaagt taaataacaa cagcattatt attgccatga cagcttttgc attggaagat
     3601 gataaaaagc aatgtcttga tgcaggcatg aatgattaca cgactaagcc aatcagaatt
     3661 gaagttgtac aatcattatt ggaaaagtgg ggtgacgtga ttttaaaaag taaggctgca
     3721 cgaaatgatg agcctttaat tgacaaacaa accattgaaa gacttaaggc aatggccgta
     3781 ggagatgata cttcttttgt aaaaaatata tttgaacttt ttcttaaaca gatggataac
     3841 ttacatgctg atatgagtaa atttttcaat gagcatgatg ccgatggtat gtataaagct
     3901 tcgcataaat taaaaggaag cgctctcaat attggtgcaa aaaggttatc tgaagtttgc
     3961 cgaattattg aagaaaaagg aaaacttggc gaccttaccg gaatgaacga attgattcag
     4021 cagttggcag aggttatttc attaacaaag aatgaaatca ggagtttaag tttggcttga
     4081 tttttaaact ttttgttgat gtaaccaagg atacaaaagc ccttctttca ttttcgtacc
     4141 tttgcacatt attaaattaa ttaaggtatg acaaacgcag tttataatat tcctccggcc
     4201 tataatgaac cggtgttaaa ttatgcaccc ggaagtccgg agaaaatggc attaaaggca
     4261 gcacttgcag aagcccgttc caaacaaatg gatataccca tgtatattga tgggcaagag
     4321 gtacgaacag gaaatacttc agcgcttcgc cctccgcacg atcatcaaca tattctgggc
     4381 atgtttcata aaggagaaaa gaagcatgtg cagttagcga ttgatgcagc acttagggct
     4441 aaaaagcaat gggcagaaat gccttgggaa caacgtgcag caatttttct aaaagcagct
     4501 gacttacttg caggccctta tcgctataag atgaatgctg ctaccatgct tggacaatca
     4561 aaaaatgctt atcaggcaga aattgattca gcatgtgaga tgattgactt tttgcgtttc
     4621 aacgtacaat atatgcgtga aatttataca caacaaccac ctgtagtagg taagggtgta
     4681 tggaaccgta ctgaacagcg tccactcgaa ggatttgttt ttgctttaac gccattcaat
     4741 ttcacagcca tttcaggtaa tttgcctaca gcaccggcaa tgatgggtaa cactgttgtt
     4801 tggaaacctg ccaatacaca aatttatgca gcgaatgtgg taatgcaatt gttgattgaa
     4861 gccggtttac ctgcaggagt aatcaatctt gtttatgttt caggccccga tgcaggtgat
     4921 gttattttta aacatgctga ttttggtgga attcatttta caggatcaac agaagttttt
     4981 aagggaatct ggaaaactat cggagaaaat atgcctttgt acaagtcata tccccgaatt
     5041 gttggcgaaa caggagggaa agattttatt atagcgcaca agagtgcaga tattgatgta
     5101 ttagtgactg cattggcacg tggagctttt gaatatcaag gtcagaaatg cagtgctgca
     5161 tcacgttgtt atattccgaa gtcaattttt gaagaggtca aaagcaagct ggttaaagaa
     5221 gtaaagtcaa tgaaacttgg accggttgaa gatttccgca acttcgtaaa tgccgttata
     5281 gacgaaaaat cattcgataa aatttctgct tacattgaaa atgcaaagaa gagtaagagt
     5341 gcagagatta ttgcaggtgg aaactttgat aagagcaaag gatattttat cgagccaacc
     5401 attatcctta catccgatgc aaaatataca accatgtgtg aagaaatttt cggccccgtg
     5461 ttgactatgt atgtttatga ggatgataag tttgaagaga ctttgcattt agttgacaac
     5521 accagcgagt atgctctaac aggagcaatt atttctcaag accgatatgc agttgatgtt
     5581 gcaacacgag cattggtaaa ttctgcaggt aacttttata ttaatgataa atgtacaggt
     5641 gctgttgttg gccaacagcc atttggtggt gccagaggtt caggaactaa tgacaaggca
     5701 ggtgctatgg taaatttgtt gcggtgggta tcaccacgaa cgataaaaga aacttttgtt
     5761 ccggcaaaag attaccgata tccttttatg gaagcggaat aaaaatttat ttagacacgg
     5821 aagtagtaga ttcgtcaagg gaaattcttc ctcctttggc gaatcttttt ttatagtaag
     5881 gttcattaag gtcacttaca ataacaccac ttgatgtgct ggagtgtaca aatttgtcct
     5941 gacccaaata catacctaca tgtgaaattc ttcgtccacg ggttttaaaa aataccaaat
     6001 cacccggagt taaattttta agcgaaacat gtggcagatt gttatacatg tcaacagagc
     6061 tgcaaccgaa tgaactgcca taaacagctt tgatgataac gttaacaaat ccggaacagt
     6121 caattccatt ttcgtttttg ccggcatatt tatatggagt attcagccaa gtgtctataa
     6181 gatcaaaaac ctttgtgtta aatacttcct taacctgata gcccaaaatc tgactgtagt
     6241 aatttcgcag gttgtcttca acaatatttt cagattgctg cgctttggca ctttgtccaa
     6301 aatacaacaa tgtaaaaatc attaaaccta ttgtgcgaaa cttcattggg caaagataac
     6361 attttaatga acgcattctt ttagcaggta ttgtctaaga tgaaaaagag ccgtcatcaa
     6421 tctgataacg gctcttaaca cttatgataa taaaaaccaa ttattcaaga ataacttttt
     6481 tcatcacagt ttttccatca aaaactaatt gaatcatata aacacctctt gcataattgc
     6541 tcacatcaac tttaccgttg tttagtcctt cgttaaattg atagctttct ttatttaatg
     6601 tgcgtcctga caaatctaac agcgttaatg ttgctgtgtt cttttcagat gcagtcacat
     6661 taaatgttac aaatgaagtt gcaggattag ggaagaaagt aacgcttgca tcatccgatt
     6721 caatacgaag tggttgttct tctaactctg acggcatttg tgtagttaat ggagtaatat
     6781 ttaaaccttc attactatta ccttcgccca ttcttgctgc gggagttgta aactgattgt
     6841 aagcactcca tgaagagtat gtgctgccac acaatccacg tacggaccaa cgataaggag
     6901 ttgatggtga tgctccaatg gtaattgagt ttacaccact tgataatgca ttagaataat
     6961 aaacatatcc ggtattcaga ttttcaactt taatgtaata gttatttgca gaagtcagat
     7021 tccagtttat ctctgcagac gttggtctga ggtttgttac atgtaaattt ccgggtgttg
     7081 ggcaagaaga accacaattg ttaactacaa cagcagtagc tgatgatgat ttagtacaac
     7141 cattacttcc tgttacggta acaatataat tgccggcagt agatgcagaa atagattgtg
     7201 atgtagagcc attactccaa aggtaattag caccattgct tgaggttaac gtaacacttg
     7261 atccctgaca gattgtggtg ctgccacttg cggtaatagt tgctgaaggt gcaggcatta
     7321 cattgacatt tacaactgat gatgttccta cataacctgt tgtaaattgt gttaccgtta
     7381 cagcataact tccggctgtg gtaactgtaa ttttacgcgt tgttgcccct gtactccata
     7441 aataagagga acctttagtt gctgttaatt ttacgttacc accggaacaa aagctcaatg
     7501 aaccgctgac agttattgta ggttgaaacg atgaatttac tgttacagtc atgggtgctg
     7561 atgtattact acaaccattg ctgtttgtta ctttaactgt ataatttcct gctgttgaaa
     7621 cagtgattga ttgtgttgtt gcacctgtac tccatagata acttccacca ctaccggcac
     7681 ttgaggcggt aagttgaaca cttccacctt gctgtaagtt tgttgaacca tttgctgtaa
     7741 ttgttgcagt tggcaaggca taaacagtta ctactttatt tgttgttgac tgacaaccat
     7801 tgtttgtaat cgttactgta tatgtacccg gagatgacgt agtaattgca ttagaagttg
     7861 cacctgtact ccacatatat gaagttcctg tgcttgctgt taacgttaca ctacctccac
     7921 tacagaacga agtacttcct gatgctgtaa tagttgcctg agtaggtgtt gttactatta
     7981 cattaactgc tgctgctgtt gcggaacctc ctgctgttgt tgctgtacat gtataacttc
     8041 ctgctgtggt aacagtaata gattgtgttg ttgcaccatt actccacaga tatgaatttg
     8101 ctgcactgca agttaatact acgcttccac cctgacagaa agttgttggt ccgttagctg
     8161 taatttttgg tgttggtgct gtagaagtta cagtaactga taatggtgat gatgttgcag
     8221 aacaaccact gttgttagta acagttacaa catagtttcc ggttgtgcta actgtaactg
     8281 cttgtgttgt cataccaccc ggtgaccatt tgtaacttcc accggtacct gctgaagaag
     8341 aagttaatgt aacacttcca ccggcagcaa aagtagttgg accacttggt gaaattgtgg
     8401 cagtaggaac tggcttaact gttactgctg ttgatgcaga cattgcagaa caaccattag
     8461 agtttgttac tataacattg tagtttccgc ctccaatagc tgtaattgac tgtgtagttg
     8521 caccactgct ccacaaatat gttgagccgc tgcttgcagt gagagtaaca cttccgccaa
     8581 cacaaaaaga agttgagcca tttgcagtga ttgttgcatt tggttgtgca taaactgtca
     8641 ctgtaacagg tgctgatgtt gctgttcctg cagcagttgt aacagtaaca acatacgaac
     8701 ctgcactggt tacattaata tatggatctg ttgaaccatt gctccataga taagagagac
     8761 cattactggc aataagattt acatttcctc ctgcacagaa cgcaacaggt ccgcttgcac
     8821 tgattgttgg cactgcaaca cctgaacagg gtgcagggct tgtataaata gcattgctgc
     8881 tggttgacaa atgaatctgg tcaatctcaa gattagtatt ggttggtgta atttttaaaa
     8941 tgtgatctgc taatgatgtt acagaggtga atgaaattac tgcgcctgag tttacatcat
     9001 atttatacca ttgccaatta gcatctgtaa tacaaccaat ttgtttgctg ttgctaccat
     9061 ctaaggaaat tgtcattcct gtagtttgtg agtttttaga acgtgctcta acccaaacat
     9121 aatacgtata gtctgttgta gatcttggct ggaagttgta agttaaatca cttacagaat
     9181 taaaacctac agcagtggtg aaatctgtaa agtcgcctgc tgctgctcct gcaggacggg
     9241 ttgttttcag atatacatta ctgttgctgt tgtcatttaa ctctgcttca aatttaaaat
     9301 cttttgacca ataatatgga taagtatttt catgccatcc atctaactga tagaatattg
     9361 gtgcagaagt gttggtgtta tcataaatat atgtactacc ttgtcttctg attttaaatt
     9421 taattctact gccatctaaa ttaatttgtg caacagcttc atcaggtgca gcacctgcca
     9481 tattagacat tggttgtgat gaacctgtga tggcatattt attttgtgaa ttgtgtttat
     9541 gtacaacaac taattttccg gggtcgccag tgttgaaaga gtaagccatg taagttggac
     9601 ttgttgtact gttaggcaca tcgcccgaca tcagatatcc gtttctgaat aagtcctcgt
     9661 aacggcttgt gattgcttgt gcataaggag gaataaccaa ttgccagata taattttttg
     9721 gattctgata aggttgagac gttacgaaat aacctgttgt gaaaaattct gcacctgtca
     9781 ttgaaacagc tttaagaaag cctaaccatt gtgcaggacg aacgttttgt gtttcgtcat
     9841 tgtcccatcc cggtgaaaca gcaggtgaaa aatatttgtc tcccgctaat aattgctctc
     9901 ttcttgattc aacaacccat tgccatcctt tccatgcacc actccaatag cgccagttag
     9961 atggccaacg catataaata tcaccggttg catagtgtga accattcatg gctgtattca
    10021 ctgtgcgtac ttcagaccat ttcagattcc atgtcggctg accgtctaac tgataataag
    10081 caaattttgt attcgataaa ccgcttaagc ccatgaactg atcgcgataa gcatttattt
    10141 ttgttgctgc tctgcgacct tcataagtga accaatcaag ccctgatgaa tttttatctg
    10201 aaacaacact tggatcagat tgcataacgt tggtgtaata taaaaaagga acgacttcac
    10261 cattatctaa tatataatct ataggtctgc tcaattttgc aagtaattgt tgcatgtaaa
    10321 atttctgagt aagtccatct tttaccaatg agtccggtgc acctgcagga ctcagatatt
    10381 ttgtagagcc tgttcctcca ttaggattca gatattgtcc ggaactattt tttaaataat
    10441 acttatcggg taaacatcca caagttgcat aaggactatt gcttttataa cctgcatctg
    10501 tcgggcgcaa ttgtggccaa tatgataatg cagatacttt atattgtgga tagctgtttg
    10561 ctagattaat ccatgcatgt gaaaaagtag ttgagtttga gtaatcacta taggcaccgg
    10621 tattttcggc agccataatg gtgtagttaa agttttttgc aaaatctgtc tgcagtgtaa
    10681 cactgtttgc tacagctttt gaataaacag tttcataagt ctgatatcct gaaagataat
    10741 agtagcctac aaacggattg tttggaaata atgtatgtcc tggcttatat cttggtgttg
    10801 gaaagttttt caattgctca attggagtaa gagatggcga tggatgtccg ggtgcaaaat
    10861 cattgatgtt gattcctgca gttaccggac ttggcgaagg tgcaacaacc gttgggtttg
    10921 caaatgaata gtgtttgccc gccataaaat tcaaatagtg agcataaata gaattgccac
    10981 caagtgagtt gtggtaaact gcaatctcat caattgcacc ctgcaactta tagtaatccg
    11041 attgtgagtt caacatgtaa tctctgttgc cactctgtga gaaactacca cctgctatgg
    11101 tttttgaaaa tcctgcaggt aattgtccgt caacccaaac ctctttaata ccggctttag
    11161 cattatactt aaatacaata tgatgccagt tgtcatcaac ataataactg tatgatgcac
    11221 ggccaatgcc gttaagatca atttcaaaat catcataaat tggtgagcca ttagcagctt
    11281 ttacttctgt catgaagtta attgccggat aggtcatgcg caattcaagt gcgccatcca
    11341 ttctcttggc aaatactgtt gtgccaaagt tcatgcccgg tttaaaaata aactcaaatg
    11401 ttatggcaga atcaatggca agtacaccac ctctgatgat ggtactgctt gagtccaatt
    11461 tcatatactt gccaacacca cctgaagtat tggtaagtat ttgataactt gactgataat
    11521 agttcggatc aagattaaaa ttgcccatcg aatctttcat tggcgaggag ccttcgaaag
    11581 tataatacga ctttggctga taattttgag caaccaaaca agttggtact gctaacaaga
    11641 acactgcaac aatgctcctt gtaattgaga ttaaatttga tttaatcatg tgaaatggta
    11701 attagaaatt aaacataata agccccctgc gattaaccga agttttttcg cattaataaa
    11761 ttttgttgtt aaacggaata gaagaattgg ttaacgcgtc caaaaataaa tcgaattttc
    11821 acccaaaaaa gaccttttcg acttcagtac gttaaaatga tgataatcag cgcatagtgt
    11881 tattaacacc tgttaataac tcaaccttcg ccatcaaaaa aatgactttc acccaaaaaa
    11941 agcgaacttt cgcccattaa aatttggtaa aaattccggc tgaaaattga aaaacatcaa
    12001 caggttgtga gtaagcaccg gttgaccttt tttctccgtt ttcggaataa attaaataca
    12061 catcatcgta gttaaattct gtacggaaat agtcgccagc aaacataact ccaacgtttt
    12121 tatgaaaaga gtattttact tcaaagccta agttgagtgc tatatctata acagaagaag
    12181 cattttctgt aacctgtaat gtttttacac catcaaaaga ttgatatata cgcttagaat
    12241 atttgccatt agcaaaacca agcatccctc ttaatgtggc atctgtttgt tcgttaaaat
    12301 ttaatgtcgt gccaagtcct aaaagcaaac tgttgacttt ccatggataa gtagttgtct
    12361 tgtatcgtgg tcccgaaggg tttgtttcaa caagactgtt cagcaatgtt tttgcatcag
    12421 aagggttgta gaaccacaca taatcggcag caatatatag tttgctgttt gggtggtaag
    12481 aagttcgcag tcgcgaggta gcgccaagca tggcatagcc ggatttaaag ttctcatcta
    12541 acttgtcggc aaaatcactc aatggaatgt ttatgcctgc tgttagacct atctgccagc
    12601 gtgatgtttc ttcttcagta tcctgtgccg aggctgttga cgataaaaga ggaataaaaa
    12661 aagtaagcca taataatttt gcgatatgct tcatgtagcc cgaaagtttg ccctaaagta
    12721 gcaactttac aggaatcaca aaccaagcaa ggcaatttta gttttcagct tatgtatttg
    12781 cgaaactact attccctttt tgtcgaagtg cctgatgaga tattttagta gtatgattga
    12841 caatgttctt tgtttttata gtgaaacatt atgatctgcc ttctttgata ttacataata
    12901 aggtttacgg ttcccttctt agtcaatatt ctgccgttta tagcttttgc ctgtatttta
    12961 tatacatagc ttccctgttg ggctatctgg cctttataca taccatccca gcgcttgctg
    13021 atgtcattag attcaaaaat catggctccc cagcggtcat aaacctgcat ggtaaagtct
    13081 ttaacacctt cgctataagc accaaatgtt tcgtttatac catcgccatt aggagtaaag
    13141 gagttaggca cataaatagc catattaggt gtcaccgtag tttcgttaga catactggtg
    13201 atttcattgg ctccctgctt gtaggcaatg accctgtact tgcgctctgc aatgttgctt
    13261 tgtgcattta caaaggtgga tttacacaca cccaacacac acatagacat taaggcaatt
    13321 tctgcaattc ttttcatatt atttattatt tgtacagtga attgcaaatt gctttcccga
    13381 atgtggttta attgtttgaa aagcgcagct aaatcacata tgtaagattt tatcatcctg
    13441 agttaaagat ttaagcacgt ttttgtgaat tctcctctga gaaaaatata tctgaatcgg
    13501 aaagacttgt gctttttatg tgaaagaaaa acgatttcag cagccattat aaatacaatt
    13561 agaccatgta tagcatatta aaaacaagtc cttccgatat tgaagattct tattctgttg
    13621 ataagcctaa aaaacacatg aatctgccaa cgttttgaga agtttttgaa caatatgctt
    13681 gtttaatgat aaaaagtatt acttttagga aaaatttagt ctgctttaga atgaaaaatt
    13741 atagttacag tatttatttt tcatcattat tcttttcagg caagatattt ttctttcttg
    13801 tggcttctca ttccaaagaa gtgtgttgta tagataaacc gccattatat cgttcactct
    13861 aaatcttaat aacatgcaca ccattgaaca acttgaggaa atgcttgccg aacacaaaga
    13921 gtggcaaggt aaaattaact tctatcgtgc agagatagac aggatgaaaa ccgatcttgc
    13981 ctcagcagtg atggccgata gaaatcagta caacatgcca catgttgagc attaccagaa
    14041 tcaatttatt ctgcagcgcg acatattaga tattatgcgt catgatttta aacagcatga
    14101 aaataaaatt gaagcacatt cttcaaatcc ggtgacaaat cttgcagctg cacatcagag
    14161 tgagcgcgaa aagcttggtt ctttcgaaag atattttaaa gagttgcgca acgagtttca
    14221 agcttttatt cataaagaag cttttagctg agaggcaacg aagtatagat ttgtgtagtc
    14281 aagtaattat tcctgctgat tttgtttaaa agaatcagga aatagtgaaa gtgttatatt
    14341 tattgaatgg agttgttcat caataaaagc tatgttacat tttacttaga ttaaaatgat
    14401 agtcatgaaa aaatcaatta tctgtttagt gttattgttt catttaacgg ttttactatt
    14461 tgtagcctgt cgcaaaaatg attcggatga tgttattcct aatgttcaac aaacgtcaga
    14521 acaaattcgc accactatta gtggatatgt actaaatgaa atggggcaag cacttagtgg
    14581 ggtaactgtt tctacaaatg gttatatagc aactacaaat gataaaggaa tttttttact
    14641 aagtaaagtt attgttgaca aaaaccgttg tgttattagt ttttctaaat caggttttac
    14701 taaacgaaac tatgtttttc gtccaacaga ttcaagcgga cagtacgtta aattgatgtt
    14761 gttaaataac gtgcctaaca aaacagtttc aggagttaat ggaggaactg tttcactacc
    14821 taatggtgca acaatagaat ttcaaccaaa ttcttttacc actgctaatg gaactgctta
    14881 tacaggaaca gttaatatat atctgaaaca tctttcacct gatgatacgg actttagtat
    14941 gatgatgcct ggtggagata tgcttgcaaa agatacaact ggtaagattg taagtttgta
    15001 ttcatttggt atgatgatgg cagaacttac atcaaccaat aatttgcctt tacaacttgc
    15061 tgcaggtaaa ccggcaacaa tctcaatgcc tattgctcct tcacaaatat taaaggcccc
    15121 tgctactatt ccattatggt attttgatga aacaacatcc ttgtggattg aagaaggagg
    15181 tgcagcaaag gtagggagtg cctataccgg gcaagttaaa catttcagtt tttggaactg
    15241 cgattttcca ggtgaaagag ctactattaa aggaaggata attgattgta acggtaatcc
    15301 catgccaaat gttgtggtaa cattagataa ttggtttgat atgttaacag ataataatgg
    15361 tgtctatcaa agttgggtac ctgcaaatat aagttacgtg ttgcaagttt tattaagtca
    15421 taactcagga gtcttttcaa gtgcacctgt caacatcaca ccaattgctc ctggccagac
    15481 ttatacagta cctgatattt cattgccttg tccttcttca attacaggaa cattgcagaa
    15541 ttgcgcagga gtaaatgttg cgggatatgt tgccgtatta aatttaaatg ctaatttatt
    15601 aaatttaatt tatacaggca gtggacaatt ttcaattaat gtgccatcac aaataccaat
    15661 agttatatat gtctcttcac tgaacaactt tgaacattac gatacattaa atgcaatcag
    15721 taatatgggg acaataataa atttaggttc aattcaactt tgtacttatg ctaatgacac
    15781 tacaaattta attacaataa atggtgcagg atttaataat cagactttta tttttactaa
    15841 tgctttattt atgacttctg atttaccttc agatacctca atatattctc atgaacttga
    15901 gttgggggga attctccaac cttatggcag ttgttattat tatggtatca gtttaaaaga
    15961 cactcttgca ggtacacaac ctataatcaa ttcaactcaa gttgattttc aattacaaac
    16021 tcctcaagtt gattatgcaa taaatttatt acctggcgga ataatagaat tacattcgaa
    16081 cgcattaatt ccaggtgatt acttcgaagg aacctattcc ggtcaagccg atataataaa
    16141 tttgaataca gtcacaagta cacagggaac ggttatagga aagtttagag tacgaaaaca
    16201 attttgattc gtataagaaa taaaaattcg agataaattg ttataatgaa agaaataaaa
    16261 caatgtagcc gttacacttt tttgagtaac ggctactatt tatatattta tattctaaaa
    16321 ctttttactt ttttttctaa aaagctttgc ctgattcgtt gttatttttt atcagtaaaa
    16381 acaaatttac tacatagcat aacagatttt caattgttcc agaagtctta atttccaaaa
    16441 caaattttta cacaaacacc aaatagacta agtaggccat aaagccaaga atcagcaatt
    16501 cttcagcaaa aaacagcagc attgtatttt ttcgtccaaa aaaagatttc ttaaagtaat
    16561 gcgagcggaa tgagttttct tccttggtgt gatcataggt gataattgga tcgttgttca
    16621 tcatagtcat aagaatttgc aggcaattta tatctgcact ttgccggaat gggataaaaa
    16681 atgaatttct gtttaagatg taagaaattc agaaaagtaa ttggtttttt tgataccctt
    16741 tttttgtgac gaacagattt ttcagagaga aagatgtttt aaagaaaaaa aattcaatct
    16801 ttagaacgca tgaaagaata aacgatacaa gctatgtttt gcagttcaga ttttgaaatc
    16861 ttcaacggaa agccacttat tgcacaaaaa caattcgtca tcaatgtggt ttgaacagat
    16921 aaaaatcaat ctgccatctg aatgtgttct taaaaattgc tgataccatg caatgttttg
    16981 tttgtcgagg ttggtgcaag gttcatctaa aagtaaaagt ggcgtgtctg atgcaatggc
    17041 aagcagtaac ttggttcgct gtttcatgcc agaagaaaat tcactcaaca atttatttct
    17101 atgtgcttcc aggccggatt cagtaatgat agaagaaata ttaaatgctg gccgtaggtt
    17161 tttaaaacgg aaataatagc ggatcatttc ctctaaagta aaatcttcaa taagcattaa
    17221 gtagggtgag gctattgaaa aataacgata gacagagtcg gcctctattt ttttatcatg
    17281 ttcatgccac tcaatttttc cttcattagg tgatacatag cccgaaataa cctgaagtaa
    17341 agttgatttg ccagagccgt ttttgcctgt tatgccaata acatcaccag aagaaaaagc
    17401 agcagtacat tttctgaaaa tccagtgttg tttgaatttt tttccggctt cagttaagtt
    17461 aacagtaaac acgatgtgat tttttgttgt tcaattattc atcatcgctg tctgatggag
    17521 taaatccacg catcaaacct ttaggcgaat tatgaataaa ttcaacaatc tggcttcgca
    17581 atgcactttc aggaatttct ttttcaattt cggcaagtgc ttttttaacg ttactgttct
    17641 gaacaaacaa tatgcgatag atatcctgaa tgtgatttac atcttcatta gaaaaacctc
    17701 tgcgtctgag tccgatattg tttatgccaa tatatgaaag tggttcgcgt gccgccttag
    17761 taaatggcgg aacatttttc cgcactaacg atcctcctgc aataaaacta tgtgcaccaa
    17821 tatgtacaaa ctgttgcact gcaaccaagc cttctaatat tgcccagtca tcaattaata
    17881 catgtcctgc aagcgtaacg ttgtttgcca tgataatatg atttcccaac tgacaatcgt
    17941 gtgcaatgtg tgcataagcc atcactaagc agttactgcc caatgtggtt ttcattttgt
    18001 ctgttgttcc tttattcatg gtaacaaact cacgaatggt agtgttgtca ccaatttctg
    18061 ctgttgtctg ttcgccttta tattttaaat cctgaggaat ggcagaaatt actgcaccag
    18121 gaaaaatgcg acagttgctg ccgattcttg caccatcata aatcgttacg tttgaagcta
    18181 tccatgtgtt gtcaccgata acaacatcat cgtaaaccat ggtaaaaggt tcaatggtta
    18241 cattgttacc gatttttgct ttagggctta ctactgcaag aggtgaaatc atgatttttt
    18301 tacaatttga gccatcattt ctgcttccat aacaactttg tcgcctacaa atgcctgtcc
    18361 gcccatgtgg caaaggccac gtctgatagg tgaaattaaa tcaagcttaa agacaacagt
    18421 atcgcccggc ttaaccatat tcttaaaacg tgctgattct atttttaaaa agtatgtcca
    18481 gtaatttcca ggatcaggaa cagtattgag tacaagaata ccacctgttt gtgccatagc
    18541 ttcaattagc aacacaccgg gcattacagg attacccgga aagtggccct ggaaaaacca
    18601 ttcgttggca gttacatttt ttacacctac aacacctgtt tcagtcagtt cagtgatttt
    18661 gtcaatcatc aaaaacggaa aacgatgtgg caggaaagat tcaattctgc taatgtcgaa
    18721 tagtgcaggt ttatttgcat caatgatggg tgcctttaaa cggtttttat cttttctgat
    18781 aatttcttta aactgttttg cgaaagcaac gttggcagca tgccccggac gcgcagcaag
    18841 aatatgtcct ttcagatgca caccaagtaa tgcaaagtca ccaacaatat ctaacagttt
    18901 atgccgtgca ggctcattct gaaaatgcag ttcaatatta tttagaatgc ctttatgttt
    18961 tatctcaact tttggtttct tgaataaggt tgccaactcg tctaacttat cctgttttat
    19021 ttcgcggtca acaattacaa ttgcattatt caggtcgcca ccttttatta aatcatgttt
    19081 aagcagcatt tcaagttcat gcaggaatac aaatgttctg cattttgaaa tctcatcttt
    19141 aaactcagta attttatgca tttgtgcatg ttgtgttccc aacacaggtg agttgtagtc
    19201 aaccattacc gtaatacgat agtcggggct tggcacagca agcatttcaa cattgcgggc
    19261 aggctcttca taggtgaggt tttctgtaat aacaaaataa ctacgtgttg cttcttgctc
    19321 acgcaagccg gccttttgaa tggcatttac aaatggaaac gcactgccgt caagaatagg
    19381 tacttcaggg ccatcaattt caataagcac attatctact tccatgccta ccaatgcagc
    19441 taaaacatgt tctgttgtac tcacctttac accgtttgca cttaaagttg ttccgcgtga
    19501 cgtatccaca acattgtcca catcggcatc aataattggt tggccgttta catcagttct
    19561 tttaaacttg tatccatgat tttcaggtgc aggcttcaat gtaagtctgg catttacacc
    19621 ggtgtgcaac cccggacctt ctacagttat ttcttctttt aaagtaactt gttttacact
    19681 catgatttat tttcagcagg gtttagacta cctgtctttt tttctaactc gttaattttt
    19741 ctttctaatt gtggtaactt tttaaacaca acgtaggtgc gtttgtagtc gcttatgtta
    19801 aatgcaggag aaccttgaac aatttcgtta ggtgttgtga tgctggttcc gatgccggat
    19861 tgtgcagcaa tttttacgcc atcagcaata gataagtgtc ctatgatacc tacctgtccg
    19921 ccaatcatac agttctttcc tattttggtt gaacctgcca ctcctgtttg tgcagcaata
    19981 actgtgtttt cgccaatttc tacattatgt gcaatttgta tcaggttgtc taacttaaca
    20041 cctttgtgta tgacagttga gcctaatgta gcacgatcaa tagttgttcc tgcaccaatt
    20101 tctacatggt cgtgaataac gacattacct atctgtggaa cttttttata gttgttgttt
    20161 tcgttaggag caaatccaaa accatctgca ccaataatta cacctgcatg gatattgcaa
    20221 tgcatgccta tctgacaaaa agaataaact ctaacaccgg gaaataaagt tgtgttgtca
    20281 ccgataatta catgatcacc aatatagcaa tgcggataaa tttttacgtt tttaccaata
    20341 actgcattct tgccaatata tgcaaaagca ccaatgtaac cttgatctcc gactgttgca
    20401 gaagggtcaa taaaagaagg cgactcaaca ccttttttat cgttaacaat ctgattgtat
    20461 atttcaagca gcgaggcgaa gcttttatag gcatcttcaa ctctgatgag tgttgcacta
    20521 attgcatttt cagccacaaa atctttattt actatgacaa cagatgcctt ggtgtcgtaa
    20581 atgtatggtg tgtattttgg gtttgataaa aaggataaag ttccgggctt gccttcttcg
    20641 attttagaca aagcatttac ttcggcatcg tgatttccct ctacagtgcc tttgaggagg
    20701 tctgctattt gtttggcggt aaacttcatt tgttactcaa taaattccgg acttggtgca
    20761 atgtgtgcaa atgtacaaat gagactgtga tatacaaact caaccaataa atactattca
    20821 actaaaaaat aatgctaaaa cagtggttgg caagggtata accaagccat ggatttgtta
    20881 atgctgaaaa ctgctatgga caaatgcctt attgcctatt ggcgctcagg ctttgtaaaa
    20941 cagcagtcag tccggcaaac atccaggcat tactccaacg cataaaagat gtcttttctg
    21001 tataattttt atattttctg aaataaaaat aacctttttg atgctgcatt ttttccagca
    21061 tgtattcagc aactttaaat gcaagggtct gttcgttaaa tcgtgttaat gtcagaagtg
    21121 attgtgctgc cgctgtacag tcaaccggaa aaacattgtt gttgtagaat ttcgggatac
    21181 gaccttcaag aaaaaagtgg ttgatgtaaa atttttttcc tgcttttagg ttttcatcat
    21241 aagtcttgtc gtcacagtgt aaaacatatt catgcaaaca atctaaaaca taaccggtgt
    21301 gatagttgtc aatccatgtt cctgcatcgc tggttgagta aatccatgca ccatctgcac
    21361 gttgatgttt catgacaaag gcaacagaat ctgctgctgc tttttttagg gattcatctt
    21421 ttgttactga ataaacctga gccaacaaac gagagccttt catgcttgca ttaaaaacta
    21481 cctgtttatc gaatggtgaa taagaaaaac aaaaatgacc attggcatcg acagtacgat
    21541 ttaaatcttt catcacaaag tctgctgcac ttttgcaaag ttgaaatgct ttttgatttc
    21601 ctgtttgttg ccaggctata aaaagtgcat tggtaattat tccggtcgca acaactgttg
    21661 gctgataggc aggtatgttg gcataacgtg cttcccagtc aaaatcatat ccccaacatg
    21721 caccatgaaa tccttttgga ataagttttt caagctcatc aataagttta gcaatatttt
    21781 ggttgagtat atttttttct tgctgattaa cattaatcag ttgtgcgtaa gcctgaatgc
    21841 aaaggccaag agtaacagga ttcagccctt tcggaacaaa aagtagtggt cgtaaattga
    21901 gtggcgatct tttaacaagt tgttgtgcgc caaagcgaat taacttattt ttattcagca
    21961 agggtaattt aaaaaatgga gattttaatg catcgtaagg gtcgtatccc ttgtagtgat
    22021 gtgactcgat atattttcgt aatcgtgatg ttgccgcctc aagggttttg gatgcatcca
    22081 ttgaattaaa gaattattct ttctccattg gaggcaatgc tttgtaatgc tcggaatgtt
    22141 gccagtgtgc tcaaaacagt ttcttcaaaa ggtattggtg caggttttcc ctgaacaata
    22201 ctatctacaa atgctttaac ctcattctga tgacctttat cctgattgcc tgtatatttt
    22261 ttttctgatt taccgaatac agtcagtgtt ttaaaatcat caagaactgc gacaatgcca
    22321 gaaccgaata cttcaagata ttctttgttg atgtttttat tgccattgct gaaataactt
    22381 accgaagcgg tactgccatt ttcaaattgc aggttaaagc aaacggtatc gtttagtttt
    22441 tgtgcatcat tcaatgcaac tgcacttata gtggtaagtt ttgatccggc aagaaacata
    22501 cataaatcaa taaagtgaca ggcttcacca acaattcgtc cacctccaac ttttaaatca
    22561 tgcacccaat gatcggcagg gattacacct gcattgattc tgtaattaat tgcaattgga
    22621 tttccggaag agaaagtttt tttaatggtt tgaatcattg gagcaaacct tctgttgaag
    22681 ccaaccatta ccctgcaatt ttgcttggta aattcatgtt gtatctgaac caattcttcc
    22741 tctgttaagc aaagaggttt ttcaacaaat acatttttcc ctgcctgtaa tgcctgcata
    22801 acataacctg catgtgtttc gtgacgtgtt gcaataaaaa ctgtattgat atcaccattg
    22861 ctgaaaatgt cctgagcatt acctgatgca aaagaaaatt gatttttgtc tgcaatgttt
    22921 cgtgcattat tgggacgtgc tgtaactata ccactgagct ttgcaggagt attctttaaa
    22981 gcaggtaata aaaaattttg tccgaaagaa cctgcaccaa tcatacctgc aaccacatca
    23041 ctcttagtat aaaaagggtt ggaaagattg actttttgtt gcagcggctt attcgtatca
    23101 tatttcaaaa ccattccaac aaaaggctcg gttttgttca taataagatc gtatgctgca
    23161 ggtgcttctt taaaatcaaa tgtatgagta agcaaatttg aaagatggat tttaccggtt
    23221 gcaattaaat caacaaaagc agccatgttt ctgttctctg tccagcgtac atagtcgtaa
    23281 ggatagtcaa ttcctccttc ttcgtattca gtatcgtaac gccccggacc ataagagcaa
    23341 ctcatgcgca ggtctaattc ttttttaaaa tagtttggtc gtttaaaccc tgttggtact
    23401 gctcccacaa taactacttt gccttttctg cgacataagg ctcctgccag atcaacagga
    23461 tcgagtgagt cagtgccggc agtgatgatt actgcgtcag tgccgtagcc gttagtaata
    23521 ttgttaattg aactttcaag atcttcactg ttacgggtaa atgcagcatc aatatcagta
    23581 tgcaaagcaa ggtcaaccat acgttggtca atatctattc cgatagtttt tatgccaccg
    23641 gctttaagca tctgaacggt aagttgtcca accagaccaa gcccgatgac tacacaattt
    23701 tcacctaaac gaaggtcggc ttgtcttatc ccctgaagtg ctattgaacc taaagttgta
    23761 aagcatgcat gttgaagggc aattttatcg tttaacttaa cacaaagatt tacagggaca
    23821 gcaataactt ctgcatgaac tgcactgttg ccaccacaag ctaccaaatc accaactctg
    23881 aattcagtaa catcaggtgc aacagcaata atttcgcctg ctgtgctgta acctaatgat
    23941 gatggtgcat ctaatttatt catcaccatt ttataagtgt ttaaaatgcc gaatgttttt
    24001 gcagcatcaa tcactttttt tacttcttct tttcttgctc ttgccttacc gatataactc
    24061 agtcgtgcat cttttacggt tttgccttca gtacctgcac taatcagcga ataatgattg
    24121 cgtactaaaa cctgccctga tgaaagaacc ggaaacggca cttcaagcaa ctgcattttt
    24181 ccatctttca gattttgtgt taactgttcc attttttgca gtttagattg tttatgtgga
    24241 atgattttga tcgaaccact ttctgaatgc tattaatcct tcttctaatg ttgtagcagg
    24301 actatagtta agtaattctt ttgctttact tatgtcggca aatgtatgag gtacatcgcc
    24361 tggttgatca ggtaattttt caagtaatgc ttttttgcct gcttcttttt ctattgctgc
    24421 aatcagcata ttcagattga tagttctgtt attaccaaga tttatgattt cgtaattgct
    24481 tttatcataa tgcatggctg caattatacc attcacgatg tcgcctacaa aagtatagtc
    24541 gcggctagtt tcaccattgc caaagaatgg aattggtgta ccctgacgta tcagcttaaa
    24601 aaatttatgt atagctaagt caggccgttg acgaggacca aacactgtaa agaagcgcag
    24661 agatataaac ctgataccat acaaatgagc ataagtatgc ccggtaagtt cgcatgagac
    24721 ttttgttgat gcataaggag aaataggttt caatacaaaa tcattttcgc tccatggtgt
    24781 gtttgggttg atgccataga cactactact gcctgcaaaa acaaactgtt tgatattttt
    24841 ttcttttgcc ttctccaata agtttacagt tcctttgata tttacatctt cataaagcaa
    24901 aggtttttca atactcggtc taactccggc acgtg
//
LOCUS       DAOOOI010000027        24397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_379_length_24397_cov_14.033358, whole genome shotgun sequence.
ACCESSION   DAOOOI010000027 DAOOOI010000000
VERSION     DAOOOI010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 24397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24397
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_379_length_24397_cov_14.033358"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PKX59_05235"
     CDS             complement(<1..730)
                     /locus_tag="PKX59_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOZ90305.1"
                     /translation="MTLKVRTMADFLAANESPDILFWVGCAGSFDQRAQKITKAVAKI
                     LNSCNISFAVLGTEENCNGDPAKRAGNEFLFQMQALMNIQIMNGYNVTKIVTACPHCF
                     NTLKNEYPQLGGHYEVVHHSMLLKQLINDGKLIINGGKFLGKKIVFHDPCYLGRGNDV
                     YEAPRDVIKKLDAELFEMKRSRANGFCCGAGGAQMFKEPENGTKDVNIERTEEILEAK
                     PDYVAVGCPFCMTMLTDGVKNFNKE"
     gene            complement(748..2052)
                     /locus_tag="PKX59_05240"
     CDS             complement(748..2052)
                     /locus_tag="PKX59_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOZ90306.1"
                     /translation="MIQGIIFTIVLATGIFFFGKSIRRIRRNIFLGKPLDRSDNASQR
                     WMTMIRVALGQSKMTVRPIAAFFHLIVYAGFIIINIEVLEIMIDGIFGTHRILSFMGS
                     FYDFLIGSFEILAVLVIIACIVFLARRNILKLKRFWSKEMTSWPRSDANIILFTEIIL
                     MSLFLTMNACDYLLQQSGAEHYIKAGSFPISSSISNLFIGMSASSLIFIERFCWWAHI
                     IGILAFMNYLPFSKHFHILMAFPNTYYSNLNPKGRLTNLESVTNEVKLMMDPSLPPPP
                     PSDTPMRFGARDVRDLSWKQLMDAYTCTECGRCTSVCPANITGKLLSPRKIMMDTRDR
                     LDEVGKNIDKYGKEHDDDKALLNDYISPEELWACTTCNTCTEACPVNIDPLSIIIDMR
                     RYLVMEQSQAPAELNGMFTKIENNQAPWQFSPADRLNWKNED"
     gene            2130..4382
                     /locus_tag="PKX59_05245"
     CDS             2130..4382
                     /locus_tag="PKX59_05245"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HOZ90307.1"
                     /translation="MKNGLLFLMIYFLPSFCFLSEKIRFEGNAMAMPTEGTDSLNIKL
                     AKTKDDKTKVIVYCELMVEYIDNNLDTAFMFLNPALQLAEKIKWDEGIARVKIEGGRL
                     FWRKGSFETALKYHNDALSYFINQEDKNSIAELITFIGQDYADWGKYQEALEYFVKAD
                     SIYRLNGNENKLAGIYLLYSFIYQSQGDYTEALKVNYKALKIYEANGDKYGAAVSLMN
                     IADSYKSLGNYQDALKSYFNGVKVFENNNDKFNLASTYSSIAEVYKLKGNFEEALKYN
                     SKTLAIAKGVNNGYLIATATQSIGEVYFQQQNYKEALGYFITSAESYRSVSDYLDLSL
                     ILSKVGLCQVKLGNFEDAKKHLDEAYSIAQTLDSRLTYTAYYQGMELLDSATGNWQGA
                     YKNHKEYISNRDSSINLENTKKILQQQIQYASDKKDALLKAEQDKKDAVQKVFRYSML
                     AGALTLLVLLIVVYNRYQIKTKTNAKLQDAYDNLQKTQLQLVQQEKMASLGALTAGIA
                     HEIKNPLNFVNNYSEVSHELIDEFLESKDEKEKVEIAQILKQNLEKIAEQGKRADSIV
                     KNMLKHSRTGSSEKQLTDINLICDEYLDLAFHGMRASTLGFRCELVKNLDPNLPKVNC
                     IGQDISRVILNLINNAFDEVYECDHLGLKGGESYQPTISIKTAVSSPHTNKAGVKYVT
                     IAVGDNGRGVPDYVRQRIFEPFFTTKPTGKGTGLGLSLSYEIIESHGGKIDIDSKEGE
                     GTNFIISLPC"
     gene            4574..5788
                     /locus_tag="PKX59_05250"
     CDS             4574..5788
                     /locus_tag="PKX59_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ90308.1"
                     /translation="MKNIKIGILREEKIPHDKRVPFSPEQCKVIKTEYPEVDVVIQPC
                     NYRAFRDDEYRREGIVLQEDLSDCDILIGIKEVPKDKLIAGKKYLYFSHTIKKQPHNR
                     EMLKKIIRDKITLVDYECLVDDHENRIIGFGRYAGIVGAYNGIMAYGLKYGLFNLKAA
                     HQSFGIEELHKELDSIKLPNIKIVITGGGRVANGAIETMGMLNIRKVTPFEFLNYSFR
                     EPVYVQLHSENYYERKDGSDFLKADFYHYPEKYNCTFCDPGSYSSFTDLLVHCAYWNP
                     NAAVLFTKEDMRSPAFRISVIADVTCDIDGSIPSTHRATTIDDKFYGYNPMNEKEEEA
                     FSTDTITVMSIDNLPCELPRDASEGFGKHLIERVIPLLIGEDKGNVIYRATIARNGEL
                     TPRFAYLTDYIS"
     gene            complement(5792..6439)
                     /gene="rpe"
                     /locus_tag="PKX59_05255"
     CDS             complement(5792..6439)
                     /gene="rpe"
                     /locus_tag="PKX59_05255"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOZ90309.1"
                     /translation="MHLIAPSILSADFSQLSKDVEMINNSVADWFHVDVMDGVFVPNI
                     SFGFPVIKAIKKHAKKPLDVHLMIIKPERYVEQFREAGADVLTVHIEASVHLHRTIQQ
                     IKKEGMKAGIAINPHTPVASLSDIIFEADLICMMSVNPGFGGQKFIENTYRKIQELKE
                     LIAIHNSKTLIEIDGGVDLNNYKKLIAAGADVLVAGNTVFSSSDPKATIEVLKKG"
     gene            complement(6530..6916)
                     /gene="apaG"
                     /locus_tag="PKX59_05260"
     CDS             complement(6530..6916)
                     /gene="apaG"
                     /locus_tag="PKX59_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HOZ90310.1"
                     /translation="MSTEVTAGIKVTVETFFQPTHSNPVQHHYVFAYRITISNESEES
                     IKLKRRHWYIIDSDNARREVEGEGVVGEQPVIEPGQLYRYVSGCNLNTEIGKMYGTYL
                     MERLSDNKMFYVKIPEFRMVVPSKMN"
     gene            complement(7195..7656)
                     /locus_tag="PKX59_05265"
     CDS             complement(7195..7656)
                     /locus_tag="PKX59_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90311.1"
                     /translation="MMKSAGIAVFILLFAGMAMAQPGQKKMDRRDKAEAMKIGFITNR
                     LSLTQDEAKKFWPVYNQFQDELSSLRQKRRGDRREAKQDFDQLSDKEVEKVVDDEIVF
                     RQSELDIIKKYHAQFKQVLPIKKVARLYQAEEDFKKELLRQLKDRRDARGN"
     gene            complement(7658..8092)
                     /locus_tag="PKX59_05270"
     CDS             complement(7658..8092)
                     /locus_tag="PKX59_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90312.1"
                     /translation="MKDEIYNELKDKAPLLSSIEKKHLFDTPENYFDQLPSAIQQKIK
                     QPQTSTIKTLFSRLLKPQFAIGLSVILLVGGFYLFNQHKADNQYAFVAVNVDSLNVNE
                     LSDLLDTDQLESIAPADNNADAETINYLIENYNDLSYDINDI"
     gene            complement(8123..8668)
                     /locus_tag="PKX59_05275"
     CDS             complement(8123..8668)
                     /locus_tag="PKX59_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOZ90313.1"
                     /translation="MNHYSDKELLEQFSNGNADYAFNLIVRKYQQKIYTHVRRLVADH
                     DDADDLTQTTFIKAWKALGNFRADAQLYTWLYRIATNECYTFLKNKRKRFFIPIHDVE
                     AELADKLEHEPSLKANELELKLQKAILSLPEKQRAVFNLRYYDEMKYEDMSEVLGTSV
                     GALKASYHHAVQKIEKFLQSF"
     gene            complement(8733..9701)
                     /locus_tag="PKX59_05280"
     CDS             complement(8733..9701)
                     /locus_tag="PKX59_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOZ90314.1"
                     /translation="MKKFTYTEKKDTRSGFGAGLLEAGKANPNVVALCADLTGSLKMD
                     AFEKAFPERFFQCGIAEANMMGIAAGMTIGGKIPFTGTFANFSTGRVYDQIRQSIAYS
                     GKNVKICASHAGLTLGEDGATHQILEDIGLMKMLPGMTVINPCDYNQTKAATMAIAKH
                     FGPVYLRFGRPSWPIFIPENTPFEIGKALLLSEGSDVSIFATGHLVWKAIEAGMALAD
                     MGISAEIINIHTIKPLDEKAVIQSVSKTGCAITAEEHQINGGLGDSICQLLSRHLPSP
                     VEMVGVKDTFGESGTPDALMTKYGLDSKNIVFAVEQVLQRKKELAG"
     gene            complement(9709..11109)
                     /locus_tag="PKX59_05285"
     CDS             complement(9709..11109)
                     /locus_tag="PKX59_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOZ90315.1"
                     /translation="MSVIKSFMQHFRKLFFLAAFFIAFTSTVRAQKNNSLPLTRIEFL
                     FDASQSMYAQWQTNTRFEIAKKLLSEMVDSLDKITNVEMALRVYGHTKKFPPQDCDDT
                     RLEVPFGKNTAYLIKKRLSEISPSGTTPIAQSLDQCGNDFPTDPARNIIILITDGIEE
                     CNGDPCAVSLMLQRKGIILKPFVIGLGLSKEYKDVFNCVGNYYDAVDETGFRTVFNVV
                     ISQALNNTTIQVNLLDEKHRPTETNVNMTFYDTYSGALRYNFVHTLNAKGLPDTLLID
                     PLGTYDIVVNTIPPVRKDSNKIVAGKHNTIGIDCPQGDLFLKFDGTSDYKNLQCIVRK
                     KGDSKTLHVQAFNTTTRYLTGDYDLEVLCLPRMIINDVNIAQSKTTTVQIPNPGMATF
                     QTVSAGYGSVYQEKDNKLNWVCNLSENNMHESILLQPGNYRVVFRPKISKEAIYTIEK
                     TFRITSGSGITVNINK"
     gene            complement(11114..11959)
                     /locus_tag="PKX59_05290"
     CDS             complement(11114..11959)
                     /locus_tag="PKX59_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOZ90316.1"
                     /translation="MPTTEQLIKISTQVRRDIVRMVHAHQSGHPGGSLGCADFLTALY
                     FEIMKHNPAGFSMDGKNEDIFFLSNGHISPVWYSVLARSGYFDIKELSTFRSINSRLQ
                     GHPTTHEGLPGVRMSSGSLGQGLSVAIGAAQSKKLNNDSSVVYSLHGDGELQEGQIWE
                     AMMYAAAKKIDNLIATVDYNGQQIDGAVNDVLSLGNLKAKWEAFGWDVLQMNGNDMKQ
                     VVETLQNARQHTGKGKPVVILMTTVMGYGVDFMMGSHKWHGIAPNDEQLKQALAQLPE
                     TIGDY"
     gene            complement(12182..12307)
                     /locus_tag="PKX59_05295"
     CDS             complement(12182..12307)
                     /locus_tag="PKX59_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90317.1"
                     /translation="MGHHHVEEKGQTFLYEGGTGAFTAVWMILVFAFIFFLIYLG"
     gene            12515..13303
                     /locus_tag="PKX59_05300"
     CDS             12515..13303
                     /locus_tag="PKX59_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOZ90318.1"
                     /translation="MSSNSVLSLFKSSIGKKVIMALTGLFLSIFLVVHVSGNFQLFKT
                     DSGLSFNQYSVLMTTNPFIKTISYLLYITIVLHAIRGIMLVLENKKARPVSYAKYDGK
                     ANSKWASRNMGLLGTVILFFIVIHLNDFWYQYKFGHIPFTMYKTELTTGKLVEVKEME
                     AGFKMGSKMEEMIVGDHKYVIVKDLYKEVKDEFKEWWMVLIYVVSMAAISFHLYHGFK
                     SSFQSLGLNHRKYNGLIQSIGTWVFAIIIPASFAAMPLYFFINR"
     gene            13319..15328
                     /locus_tag="PKX59_05305"
     CDS             13319..15328
                     /locus_tag="PKX59_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOZ90319.1"
                     /translation="MTNNILDAHIPAGEIEKKWSKYRSTVPLINPANKRNLEIIVIGS
                     GLAGASAAASLAELGYKVKVFCFQDSARRAHSIAAQGGINAAKNYQNDGDSIYRLFYD
                     TVKGGDYRSREANVYRLAEVSGNIIDQCVAQGVPFAREYGGLLSNRSFGGVQVQRTFY
                     AAGQTGQQLLLGAYSALMRQVGMGAITMYSRHEMLDIVKIDGKCRGIIARNLITGELE
                     RHFGHAVLMCSGGYGNVFYLSTNAMGSNVTAAWKAHKNGAYFANPCFTQIHPTCIPVS
                     GEHQSKLTLMSESLRNDGRIWVPKKKEDAEAIRAGKLNPNDLKEEDRDYYLERRYPAY
                     GNLVPRDVASRAAKERCDAGFGVNATGEAVYLDFAYNMKYKYGKMEATMNSIENPTEE
                     QLFELGKAVVKEKYGNLFDMYKQITGVDPYHSPMMIYPAVHYTMGGLWVDYNLMTTVP
                     GLYALGEANFSDHGANRLGASALMQGLADGYFVIPYTIGAYLSSEIRTKAISTDHEEF
                     VAAENRVRETLQKLMQIKGTKSVDHFHRQLGKIMWEKCGMARNAVALKEAIAEIQELR
                     KQFWADVRIPGEMNEFNPELEKAGRVADFLELGELMCMDALQRNESCGGHFREEHQTE
                     EGEAMRDDDNFAFAAAWELTGDSQWKMNKEELKFENIKIAQRSYK"
     gene            15362..16102
                     /locus_tag="PKX59_05310"
     CDS             15362..16102
                     /locus_tag="PKX59_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOZ90320.1"
                     /translation="MNITLKVWKQRNKDERGRFETYQVQDVSEDMSFLEMFDVLNERL
                     INEGKDPVAFDHDCREGICGACSMYINGRPHGPLQGVTTCQLHMRSFKDGDTIVVEPW
                     RSAAFPVIKDLAVDRSSFDRIMAAGGFVSVNTGNAQDANSILIPKDDADKAFEAAACI
                     GCGACVAACKNSSAMLFVSAKVSQLSLLPQGKAERHERVMKMVAQMDDEGFGNCTNTG
                     ACEAECPKEISIENIARMNREYLSAKIS"
     gene            16211..16921
                     /locus_tag="PKX59_05315"
     CDS             16211..16921
                     /locus_tag="PKX59_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOZ90321.1"
                     /translation="MTASEEERIRKAFSDKDWQEIKAQDTWQIFKIMSEFVEGFEKMS
                     RIGPCVSIFGSARTKPDTRYYLLAEEIAYQLTQSGYGVITGGGPGIMEAGNKGAQRAG
                     GKSVGLNIKLPFEQTSNPFVDHDKNLMFDYFFVRKVMFTKYAQGFVVLPGGFGTLDEF
                     FEALTLIQTKKIGRFPIVLVGKEYWGKLFEWLQTVMVDEGNILPEDLSLFKLVDEAHE
                     AKSHIDAFYSKYLLKPNF"
     gene            complement(16918..17682)
                     /locus_tag="PKX59_05320"
     CDS             complement(16918..17682)
                     /locus_tag="PKX59_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOZ90322.1"
                     /translation="MLTSSEIKQIKSLKDKASRHEQQKFVVEGEKMVTELAHSAFNII
                     AVYATDEWIGQANNEILSLLNEKLIQVKTKELERISSLKQPNGVLALVAMRQQQETEI
                     KFNDIILAFDDISDPGNLGSIIRIADWFGIKQIVCSSTSVDLYNSKTIQATMGSIFRV
                     NVFYTDVEKWISANKQNATFYATMLNGKSVYSVAKKKPAVLVFGNESRGLSHSIQQLI
                     DLDISIPKFPDEKSSSVESLNIAMSAAIFCSEWRRD"
     gene            17754..20045
                     /locus_tag="PKX59_05325"
     CDS             17754..20045
                     /locus_tag="PKX59_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOZ90323.1"
                     /translation="MRKRQYGILITLILLVASCSPFKQLDKGEYLLNRNQVIIDKPEL
                     REGVKAIIKQKANRRILGLFRFHLAVYSMGNRGKPTKFKNWLKNTVGEEPEVLDTLFT
                     KKSTQQIKQFMSKNGYFDAEVKDSTIYRKKKAIVRYTIVAHKPSFIKYVARECADDNL
                     DSLVIKDTSTSKLQAGIIYNEGILQGDRERLTKKLRNEGYFQFSPQFVSYRVYTSDTT
                     KNIAVFQDVAAFSNPDSTISSAALPHTKFKLNRIIFSPDYDPLSLNTSLIQYDTVAYK
                     GYLFVKRAGKRMPVKPEVIVNHTFLKPEAVFRQSNADYTYKGLNLLGMYRFVNINYNV
                     VDSNRLDCFINLGTTPKQEYKTELEGTNNGGNLGVAGDFTYRNKNVFRGAEMFEFKIR
                     GALESQKKFTDDKENKVLLLFNTYELGVENSIYLPKAIKPFNRLFKNIAANPVTVFST
                     NYNIQNRPEFNRSILDFSASLEWRKGRFIKQIVTPFQVNFVNVKLEPEFKARLESLND
                     PVLFSSYDNHLISNGRYTLIYNNQQLNEPGNFKFFRTNIEFAGNTLYWFRQIEGTDKD
                     ANNSYTILNKRFAQYFRPDADFRYYQNINAHNSIVYRIAAGIGIAYGNSKIIPFEKSF
                     YAGGSNDLRAFRARNVGPGNFTSVNNFERTGDIKINGNIEYRFDILKILKGAFFLDAG
                     NIWVRKKESSRPNAEFEIDKFYKQLAIGSGLGFRFDFTFFIFRLDIGVPIVDPQYKGD
                     DKMVADEVKPRSLIYNFGIGYPF"
     gene            complement(20052..20438)
                     /locus_tag="PKX59_05330"
     CDS             complement(20052..20438)
                     /locus_tag="PKX59_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOZ90324.1"
                     /translation="MNIQPEKVRIDKWMWAVRIFKTRSQAAEACAKGKVFIDETAVKA
                     SRMVKTGETISIRRGSFTLVFQVIQPIENRVAAAVKDQFCKDLTSADEYEKIKMHSLA
                     VRTYRQHNEGRPTKKERRALDDFLDW"
     gene            complement(20465..22576)
                     /locus_tag="PKX59_05335"
     CDS             complement(20465..22576)
                     /locus_tag="PKX59_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HOZ90325.1"
                     /translation="MLKGFRGSTGISVHQLEQTIKLFEEGATIPFIARYRKEKTGGLS
                     DENLLELKKLLKEVEDFNNRKQFIINTLQAKGVLDSLKNKIEAATTLNELEDIYLPFK
                     EKRKNRAQVARDAGLEPLAQTLIHDDRKAKQLAEKNISSTFSDTEKVLQGARDIIAEL
                     ISENHQVRKTFRQCFEQKSFIVSKVIKSKVEEAVKYKDYFDYKEKISSCAAHRLLAIL
                     RGEKEKLLRVSVMPDSTFLLNLLFDTIKTEGFYLNTQVKTAAEDACERLLVPSMENET
                     IGHYRQKAETESARLFAVVLKQLLLAPPLGQKNILAIDPGFKSGCKIVCLDKNGRLLH
                     NETIFPHPPQSQQKIASHKISTLVSQYKIDAIAIGDGTAGRETATFIQRLRFDRKIQV
                     FVVNEDGASVYSASAEGRKEFPEYDVTVRGAVSIGRRLMDPLAELIKIDPRSLGIGQY
                     QHEIDEMMLNTALDQTIEHCVNTVGVNLETASEKLLTYVSGIGEVMAKKIIDARANSG
                     IHSRNELKNIKGMGDKTYEMCAGFIRVNGDNPLDKSAVHPENYTLVERMAKDIDTTIA
                     GLIGNDSLIQKIDISKYVSAEVGLPTLKDIIAELSKPGRDVRGIIKVFEFNTEIKSIN
                     DLQPDLLLPGIITNITNFGAFVDIGIKKDGLVHISEISDEFISDISSAVRLNQQVMVK
                     VLSADSKTGRIQFSMKGIQQP"
     gene            complement(22623..23009)
                     /locus_tag="PKX59_05340"
     CDS             complement(22623..23009)
                     /locus_tag="PKX59_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90326.1"
                     /translation="MKRTFTFLSVATAFCVVTATVITPSCKSSKSTTSTTTAVSVPDK
                     PSYAVHVKPIVDAACGTKCHSPAKKADGLDLSFYEGVKDACEKHKLLASIKHESGVKA
                     MPRMAPQLSKEAIATIEKWINDGYAQ"
     gene            23106..23699
                     /locus_tag="PKX59_05345"
     CDS             23106..23699
                     /locus_tag="PKX59_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HOZ90327.1"
                     /translation="MSYKPNDYYARKAKQENFAARSIYKLEEIDLKHKLFRQRDNVLD
                     LGASPGSWLQYASKKAGEKGKVFGIDLKPVEVKLPNVFTVQDNIETFDLQTLFDRYGF
                     NDKFDVVMSDMAPNTTGSRFTDQARSFDLSMLALQTAKRFLRPGGHFVCKIFDGEDAM
                     TFRDEVKSCFGECFVLRPKSTRNSSKEIFVTGKYFKT"
     gene            23789..>24397
                     /locus_tag="PKX59_05350"
     CDS             23789..>24397
                     /locus_tag="PKX59_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90328.1"
                     /translation="MKRILLSLAFTITGFLAFAQCSIDSTQTDPGIYPDTLLPATVNQ
                     PYTQDITFVMITDTMGITITNFNIANITGLPVGMTWQCNNFANGCNYDPSQSIYGCVN
                     LSGTPLIAGVYNATVTVIATLQVLGDQTISYTLPFTVLPDTVSNPGFSMTNSSGCLPL
                     SVTFVTNNPGQASYLWDFGNGIQSTLENPPIQTYNAPGDYVVT"
BASE COUNT         7530 a         4380 c         4663 g         7824 t
ORIGIN      
        1 cttctttgtt gaaatttttc acaccgtcag taagcatagt catacaaaag gggcagccaa
       61 cagctacata gtctggttta gcttcaagaa tttcttctgt acgttctata ttcacatctt
      121 tagttccatt ttcaggttct ttaaacattt gtgcaccacc tgcaccacaa caaaatccgt
      181 tggcacggct tcgtttcatc tcaaataact ctgcatctaa ttttttaatc acatcacgtg
      241 gagcttcata tacatcgttg ccacgaccta aataacatgg gtcgtgaaat acaattttct
      301 tacctaaaaa ttttccgcca ttgataatca gttttccatc atttatcaat tgtttcaaca
      361 acatgctgtg atgcacaact tcatagtggc cacctaactg tggatattca tttttaagag
      421 tattaaaaca atgcggacaa gcagttacaa tttttgtaac attatagcca ttcatgattt
      481 gtatattcat caacgcctgc atctgaaata gaaattcatt acctgcacgt tttgcaggat
      541 caccattgca attttcttct gtgccaagaa cagcaaagga aatattgcaa gagtttaaaa
      601 tcttagcaac agccttggtg attttttgtg cacgctgatc aaaacttcct gcacagccta
      661 cccaaaataa aatatcgggc gattcgttgg cagctaaaaa atctgccatg gttcttactt
      721 ttaatgtcat cgtgttttgt ttaattgtca atcttcgttt ttccagttca ggcggtcagc
      781 aggtgaaaac tgccatggtg cctgattatt ttctatttta gtaaacatgc cgtttaattc
      841 tgcaggtgcc tgtgattgtt ccattaccaa ataccttctc atatcaataa ttattgaaag
      901 cggatcaata tttacaggac aagcctctgt gcaggtatta caggttgtgc aagcccaaag
      961 ctcttcaggt gaaatataat catttaacaa agctttatca tcatcatgtt cttttccgta
     1021 tttatcaata ttttttccta cttcgtcaag gcggtcacgg gtgtccatca taatttttct
     1081 tggagacaaa agtttgcctg tgatgtttgc aggacaaacg gaagtgcatc ttccacattc
     1141 cgtacacgta taggcgtcca tcagttgttt ccagctaagg tcacgcacat ctcttgcacc
     1201 aaagcgcata ggagtatcac ttggtggggg aggaggtaat gatggatcca tcatcaactt
     1261 tacttcattg gtaacactct ctaaatttgt aagcctgcct tttggattca aattgctgta
     1321 ataggtattt ggaaatgcca tcagaatatg aaaatgttta gagaatggca ggtagttcat
     1381 aaatgcaagt atgccaatga tatgtgccca ccagcaaaac ctttcaataa aaatcaatga
     1441 tgaggcagac ataccgataa ataaattact gattgaacta ctaattggaa atgaaccagc
     1501 ttttatatag tgttctgcac ctgattgctg caacagatag tcacatgcat tcatggtcag
     1561 aaataatgac attaaaataa tttcagtaaa tagaataatg ttggcatcac tacgtggcca
     1621 tgaagtcatt tccttgctcc aaaaacgttt tagtttcaga atatttcttc ttgccagaaa
     1681 cacgatgcat gctattatta ccaacactgc cagtatctcg aacgagccaa tgaggaaatc
     1741 ataaaagcta cccataaacg ataaaattct gtgagtgcca aaaatgccat caatcataat
     1801 ttccagtact tcaatattga tgatgataaa tccggcataa actatcaggt ggaaaaaggc
     1861 agctatcggc cgaactgtca ttttactctg acctaaggca acacgaatca tagtcatcca
     1921 tcgttgtgat gcattgtctg acctgtcaag cggtttgcct aaaaatatat ttcgtctgat
     1981 gcgtctgatg ctcttgccga aaaagaatat tccggtagcc agcactatgg taaatataat
     2041 tccctgaatc atagatgagc ttaaatcaat tgatgcaaat gtaaaagaaa ccaaacaatt
     2101 tatttgtgtt taagtattat ctttcgtacg tgaaaaacgg cctgcttttt ttaatgattt
     2161 atttcttacc ctctttttgt tttctctctg agaaaatacg ttttgaaggt aatgctatgg
     2221 caatgcctac tgaaggaaca gattcactta acattaagtt agcaaagact aaggatgata
     2281 aaaccaaggt tattgtttat tgtgaattaa tggttgagta catagacaac aaccttgata
     2341 cagcttttat gtttttgaat ccggcattgc aactggcgga aaaaattaaa tgggatgaag
     2401 gtattgcaag agtaaagatt gaaggtggcc gtcttttttg gcgaaagggg agttttgaaa
     2461 cagcattgaa gtatcataat gatgcattaa gttatttcat taatcaggag gataaaaata
     2521 gtattgcaga gctaattact ttcattggac aggattatgc cgattgggga aagtatcagg
     2581 aagcattgga gtattttgtc aaggcagatt ctatttatcg tctaaacggg aatgagaaca
     2641 aattggctgg aatctatttg ttgtatagtt ttatttatca aagtcaggga gactatacag
     2701 aagctttaaa agttaattac aaagctttga aaatttatga agcaaatggc gacaagtatg
     2761 gtgctgcagt atccttaatg aatattgccg attcttataa gagcttaggg aattatcagg
     2821 atgccctaaa aagttatttt aatggagtaa aggtttttga aaataataat gataaattta
     2881 accttgcttc aacatattcc agtattgccg aagtttataa actaaaagga aattttgaag
     2941 aagcattaaa atataatagt aagactttag caattgccaa aggagttaat aatggttatc
     3001 tgattgccac tgccacacaa agtataggtg aagtttattt tcaacaacag aattataagg
     3061 aagcacttgg ctattttata acttcagcag aatcatatag gagcgtttct gattatttag
     3121 acctttcatt aatactaagt aaggtaggtc tttgtcaagt taaacttggg aatttcgaag
     3181 atgcaaagaa gcatttggat gaagcttatt caattgcaca gactcttgat tcaagactta
     3241 cctatactgc atattatcag ggcatggaac ttttagatag tgccacagga aattggcaag
     3301 gtgcatataa aaatcacaaa gagtatatta gtaaccgcga cagctcaatt aatttggaga
     3361 acactaaaaa aatattgcaa caacaaattc aatatgcaag cgataagaaa gatgcattgc
     3421 tcaaagcaga acaagacaag aaagatgcag tgcagaaggt gtttcgatat tcaatgttgg
     3481 caggtgcttt aacattattg gttttattga tagtagttta caaccgttat cagattaaga
     3541 ctaaaaccaa tgcaaagttg caggatgctt atgacaactt acagaaaacg caactgcaat
     3601 tggttcagca agagaaaatg gcctcattgg gtgcattaac tgccggtatt gcacatgaaa
     3661 taaaaaatcc gctcaatttt gtaaataatt attccgaagt gagtcatgaa ctgattgatg
     3721 aatttcttga atctaaggat gaaaaagaga aagttgagat tgcacaaatt ttgaaacaaa
     3781 acttagagaa aatagcagaa caaggaaagc gtgcagacag cattgtaaag aatatgctaa
     3841 aacattctcg caccggaagt tcagaaaaac aacttaccga tattaacctt atttgtgatg
     3901 agtatcttga tcttgcattt catggaatgc gcgccagtac tttaggattc agatgtgagc
     3961 tggtgaaaaa ccttgatcca aatttgccaa aggtaaattg catagggcag gatatttcac
     4021 gagttattct caatctgatt aataatgctt ttgatgaagt ttatgaatgt gatcatcttg
     4081 gattgaaagg tggagagtct tatcagccaa ctatttcaat aaaaacagca gtatcaagtc
     4141 cacatacaaa taaagcaggg gtaaagtatg ttacaattgc tgttggagac aatggcagag
     4201 gtgttcctga ttatgtaaga caaagaatat ttgagccatt ttttactacc aaacctactg
     4261 gtaaaggcac gggtttagga ttgagtttaa gctatgaaat tattgaatct catggtggta
     4321 aaatagacat agattctaaa gaaggcgagg gaaccaattt tataatttca cttccttgtt
     4381 aagaatttct aaaaattatt ttattataaa atcttacttg ttttttagac gcaatgcttt
     4441 caaatcatca gtaaactgaa agcgtatttt actccttatg cgaaagggtt acttgattga
     4501 ttttcgaata catgataaat gttgcttttc gttttaattt aaagcggtat ttttgagcag
     4561 atttttattg aggatgaaaa atattaaaat aggaatacta agagaagaga aaattccaca
     4621 cgataaacgt gtaccattta gccccgaaca atgcaaggta attaagacag aatatcccga
     4681 agtagatgtt gtgattcaac catgcaacta tcgcgctttc agggatgatg aatataggag
     4741 agagggaatt gttttgcagg aagatttatc agattgcgat attcttattg gcataaaaga
     4801 agtgcctaag gataagctca ttgcaggaaa gaaatatctt tacttttcac ataccataaa
     4861 aaagcagcct cataaccgtg aaatgctgaa gaaaataatt cgtgataaaa ttacactcgt
     4921 tgactatgag tgtttggttg atgatcatga aaaccgcatt attggttttg gacgctatgc
     4981 cggtatagta ggagcatata atggtatcat ggcttatggt ttaaagtatg gattgtttaa
     5041 cttgaaagct gcgcatcaga gttttggtat tgaagagttg cataaggaat tagacagcat
     5101 taaacttccg aatattaaaa tagtgattac aggaggtggt cgtgttgcta atggtgctat
     5161 tgaaacaatg ggaatgttaa atatcaggaa agttactcca tttgaatttc tgaattattc
     5221 attccgtgag cctgtttatg tgcagctaca ttccgaaaat tattacgaac gcaaagacgg
     5281 gtcagatttt ttgaaggccg acttttatca ctaccctgaa aaatataatt gcactttctg
     5341 tgacccggga tcatattcat cgttcaccga cttgttggtg cattgtgcct attggaatcc
     5401 taatgctgct gtgctgttta cgaaagaaga tatgcgaagc cctgcattcc gtatcagtgt
     5461 cattgcagat gtcacttgcg atattgatgg tagtattcct tcaacgcatc gcgcaacaac
     5521 tattgacgat aaattttatg gttataatcc aatgaatgaa aaagaggagg aagcattcag
     5581 tacggatacc attactgtca tgtctattga taaccttcct tgcgaattac cgcgtgatgc
     5641 ttctgaaggt tttggtaagc atttaattga acgtgttatt cctcttttga taggtgagga
     5701 caaaggcaat gtgatatacc gtgctaccat agcccgcaat ggtgaattga ctccacgatt
     5761 tgcatatctt acggattaca tcagttgatg tttatccttt tttaagtact tcaattgtgg
     5821 cctttgggtc agaagagcta aaaacggtat ttcctgcaac aagaacatcg gcaccggcag
     5881 caataagttt tttgtagtta ttcaggtcaa caccaccatc aatttctata agtgtttttg
     5941 agttgtgaat agcaattagc tctttcaact cctgaatttt tctgtacgtg ttttctataa
     6001 atttctgacc gccaaaaccc ggattcacgc tcatcataca aattaagtcg gcttcgaaaa
     6061 taatatcgct caatgatgcc acaggcgtgt gtggatttat ggcaatacct gctttcatac
     6121 cttctttttt tatctgctgt attgttcggt gcagatgaac agatgcctct atatgaacgg
     6181 taagaacatc agcacctgct tcacgaaact gttcaacata tctctcgggt tttataatca
     6241 taagatgaac atccaaaggc ttttttgcat gtttctttat ggcttttatt accggaaatc
     6301 caaaagaaat gtttggcacg aaaacaccat ccataacatc aacatgaaac caatctgcaa
     6361 cactgttgtt gatcatttcc acatctttac tcagttgtga aaaatcggca gaaagaatgg
     6421 aaggtgctat aaggtgcatg gtgaaacttt atgcaaaact aacttttatc cataaaaaaa
     6481 gagcctggcg gctctctttt taaaaattca aatcaaataa ctattgggac taattcattt
     6541 ttgaaggcac aaccattcta aactcaggta tcttaacata aaacatttta ttatcgctaa
     6601 ggcgttccat tagataggtg ccatacattt ttcctatttc agtattcagg ttgcatcccg
     6661 aaacatatcg gtaaagctga cccggttcaa taacaggttg ttctccaaca acgccttcgc
     6721 cttcaacttc gcgtctggca ttgtcgctgt caatgatata ccaatgtctg cgttttagtt
     6781 taatgctttc ttcgctttcg ttagaaatgg taatccgata tgcgaataca taatgatgct
     6841 gaacagggtt ggaatgggta ggttgaaaga atgtttctac cgttaccttt attcccgctg
     6901 ttacctctgt tgacatatac atgatgtgtt gttgtgtata acggatgtaa aattaaacat
     6961 gcatgtatgc aatgttaggt tgttttggta aatgtgcttt tttacagggc gaaggttgat
     7021 tttatctgta taggcattaa aaatgtagat ttgtttttgg tgaaaagcag agaatttgag
     7081 ttaaaaatga aggataataa ttaatcatag ttagtttttt gggtaaaatg attctttata
     7141 caaataaaaa agcgagcatt gcccgcttaa tctattacta aacattataa atgattaatt
     7201 acctcgtgca tctcttctgt ctttaagttg acgaagtaat tctttcttaa aatcttcttc
     7261 agcttgatac aatctggcaa cttttttaat aggaagaacc tgtttaaatt gggcatgata
     7321 ttttttaatg atatccaatt cgctctgacg aaaaacaatc tcatcatcaa ctactttttc
     7381 aacttccttg tcagacaatt gatcaaaatc ctgtttggct tcacgcctgt cacctctgcg
     7441 tttttgtcgt agtgaactta actcatcctg aaattgatta taaacaggcc agaatttctt
     7501 tgcttcatct tgtgttaggc tcagtctgtt ggtgatgaat ccaattttca tagcttcagc
     7561 cttatccctt ctgtccatct tcttttgacc gggttgtgcc attgccatac ctgcaaaaag
     7621 caaaataaac acagcaattc ctgctgattt catcatttta aatgtcgttg atatcataac
     7681 ttaagtcatt atagttttca ataagataat ttatagtttc agcatcagca ttgttatctg
     7741 ccggtgcaat agattctaac tgatcggtat ctaacaagtc actcagttca ttgacattta
     7801 aagaatcaac atttactgca acaaatgcat attgattatc tgctttgtgt tgattgaata
     7861 aataaaaacc tccaaccaaa agtataacag acaatccaat tgcaaattgc ggtttcagca
     7921 accttgagaa taaagtttta attgtgctgg tttgcggctg tttaattttt tgttgaatgg
     7981 ctgagggcag ttgatcaaaa taattttcag gagtatcaaa aagatgcttc ttttctattg
     8041 acgacagcaa cggtgcttta tccttcaatt cattgtaaat ttcatctttc atactacttt
     8101 gatgtttttg atttaaaaag gtttaaaatg attgtaagaa tttttcaatt ttttgcactg
     8161 catgatggta agatgcttta agagcgccaa cagatgtacc taaaacctcg ctcatgtctt
     8221 cgtatttcat ttcatcataa tacctgaggt taaatactgc cctttgtttt tccggtaaag
     8281 ataatattgc tttttgaagt ttaagctcca actcattggc ttttaaagac ggttcatgtt
     8341 ctaatttatc tgccagttca gcttcaacat catggatggg aataaaaaac cgtttccttt
     8401 tgtttttcag aaaagtgtaa cattcattgg tagcaattct atacagccat gtataaagct
     8461 gtgcatcggc tctgaaattt cctaaggctt tccatgcttt aataaaagtt gtttgcgtta
     8521 aatcatcggc atcatcatgg tcagcaacca aacgccttac gtgagtgtaa atcttctgct
     8581 gatattttct gacaatgaga ttaaatgcgt aatcggcatt gccgttacta aattgctcaa
     8641 gtagttcttt atccgagtag tgattcaatg aaaaaccgat ttatcctatt atttcaattt
     8701 aaaaagattc ccggtgtttc acagggaaaa aattagcctg ccaattcctt tttgcgctgc
     8761 aaaacctgtt caactgcaaa gacaatattc ttactgtcga gtccgtattt agtcatcagc
     8821 gcatcaggtg ttccactttc accgaaggtg tcttttactc caaccatttc aacaggtgag
     8881 ggcaaatgac gcgacaataa ctgacaaata ctatctccaa gccctccgtt aatctgatgt
     8941 tcttctgcag ttattgcaca tccggttttt gaaactgatt gtatgactgc tttttcatca
     9001 agaggtttaa tggtgtgaat atttattatt tctgcactaa tgcccatgtc ggcaagtgcc
     9061 attcctgctt caatagcctt ccagaccaaa tgccctgttg caaaaatact gacgtctgaa
     9121 ccttcgctta ggagcaatgc ttttcctatt tcaaaaggtg tattctcagg aataaatatc
     9181 ggccaggatg gtcgtccgaa acgtaaataa acaggtccga aatgttttgc aatagccatg
     9241 gtggctgctt ttgtttgatt atagtcgcat gggttaatta cagtcatgcc cggcaacatt
     9301 ttcatcaggc caatgtcttc aagaatctga tgtgttgcac catcttcgcc taatgttaaa
     9361 cctgcatgag atgcacatat ctttacattc ttgccagaat aggcaattga ttgccttatc
     9421 tgatcataaa cccttccagt agaaaaatta gcaaacgttc ctgtaaaagg aatttttcca
     9481 ccaatagtca tacctgctgc aatacccatc atgttagctt ctgcaatgcc acattgaaaa
     9541 aaacgctcgg gaaatgcttt ctcaaatgca tccattttta atgaacctgt aaggtcagca
     9601 cataatgcta ctacattagg gttggccttg cctgcttcta acaatcctgc gccaaagccg
     9661 gaacgggtat cttttttttc ggtgtaggta aattttttca ttactcaatt atttgttgat
     9721 gtttactgta attcctgatc cggaagttat acggaatgtt ttttctatag tataaattgc
     9781 ttctttagat atttttggtc tgaatactac tctgtaattt ccgggttgta ataatatact
     9841 ttcatgcatg ttgttttcgc tgagattaca aacccaattc agtttattgt ctttttcctg
     9901 atatacactt ccataaccgg cagaaacagt ttgaaaagtt gccatgcccg gatttggaat
     9961 ctgaacggtt gtggttttac tctgtgcaat atttacatca ttaataatca tccttggcag
    10021 acataatact tcaaggtcat aatctccggt gaggtagcgc gtggtagtgt taaatgcttg
    10081 aacatgcagt gttttactgt cacctttttt tctgacaatg cattgaagat ttttatagtc
    10141 gcttgtaccg tcaaatttta aaaataaatc accctgcgga caatcaatac caattgtatt
    10201 gtgtttgcct gcaacaattt tattactgtc ttttcttacc ggtggtatgg tgtttacaac
    10261 aatgtcgtag gtgcctaaag gatcaattaa taaggtgtcc ggtagtcctt ttgcattcaa
    10321 tgtgtggaca aaattatagc gtaaggcacc cgaataggta tcataaaagg tcatgtttac
    10381 attggtttct gtaggtctgt gtttttcatc cagcaggtta acctgtatgg tagtgttgtt
    10441 taacgcttga gaaatcacaa cattgaatac agttcggaaa cctgtttcat caactgcatc
    10501 ataatagtta cctacacaat taaaaacgtc tttatattct ttggaaagcc caaggccgat
    10561 aacaaatggt ttcagaataa ttccttttcg ttgcaacatg agtgaaactg cgcatgggtc
    10621 tccattacat tcttctattc catctgtaat gagaataata atgtttcgtg caggatctgt
    10681 tgggaagtca ttgccacatt gatcgagcga ttgtgcaata ggagtggtgc cactcggact
    10741 tatttcactc aatctttttt taatcagata ggctgtgttt ttaccaaaag gaacttcaag
    10801 tcttgtatcg tcacaatcct gaggaggaaa ttttttagtg tgaccataaa cacgcaaagc
    10861 catttcaaca ttggtgattt tatctaagct gtcaaccatt tcagacaata gctttttggc
    10921 aatttcaaaa cgggtatttg tttgccattg tgcatacata ctctgcgatg catcaaataa
    10981 aaactcaatg cgtgtaagtg gcaaagaatt atttttttgt gccctgacag ttgatgtaaa
    11041 ggctatgaaa aatgctgcga ggaaaaataa tttcctgaaa tgctgcataa aactttttat
    11101 taccgacata caattaataa tcgcctatgg tttccggcag ttgtgcaagt gcttgtttca
    11161 actgttcatc atttggtgct atgccatgcc atttatgtga tcccatcata aagtcaacac
    11221 cataacccat aacagttgtc atcaatataa cgacaggttt cccttttcct gtatgctgac
    11281 gtgcattttg aagcgtttca actacttgct tcatgtcatt tccattcatt tgcagcacat
    11341 cccatccaaa tgcttcccat tttgctttca ggttgcctaa tgaaagcaca tcattcacag
    11401 caccatcaat ctgctgaccg ttataatcaa ctgttgcaat aaggttatca atttttttag
    11461 cagcagcata catcattgct tcccaaatct gcccctcctg caattcacca tcaccatgca
    11521 gtgaataaac aacagaactg tcgttgttta atttttttga ctgagctgcg ccaatggcaa
    11581 cgctaagtcc ctgtccaaga gaacctgacg acatacgaac tccaggcagg ccttcatgtg
    11641 ttgttgggtg cccttgtaaa cgactgttga tgcttctgaa tgttgataat tcttttatgt
    11701 cgaagtaccc acttctggct aaaacactgt accacacagg agaaatgtga ccgtttgaaa
    11761 ggaaaaagat gtcctcattt ttaccatcca tgctaaaacc tgcaggattg tgtttcatga
    11821 tttcgaaata aagtgcagtt agaaaatcgg cacaacctaa agacccaccg gggtgtccgc
    11881 tttggtgggc atgtaccatg cgaacaatat cgcgtctgac ttgtgtgctg attttaataa
    11941 gctgttctgt tgtgggcatg agctaaaaat tatgctccaa aactcggtaa aatgcttgtt
    12001 gaacaaaaag gaagttttta acaaaaaagc attgttatta tggaatggac gcagagaaat
    12061 atatcctgga atctgtgaat atagtggagt aataaaaact aatgaaagaa tatgtccaca
    12121 cagccacaat tttgtggaat ggttaaaaaa agtcagcctg ttttggctga taaatttaaa
    12181 attatcctaa gtaaatcaga aagaatataa aggcaaaaac caaaatcatc catactgcag
    12241 taaatgcgcc agtgccgcct tcatataaaa aagtctgtcc tttttcttct acgtggtgat
    12301 gtcccattgt ttcaaataat ttttgcaaag ataattctcc gaaacttaat tcgcaatttt
    12361 ttgatgcagc aaattgttta tcctattatt tatgatgagt gttgataaaa atcatacagt
    12421 tcaattgttg aaatatactg agtcatgaat tgcaattggt tttcgtaatc tctacatttg
    12481 cgcatcaatt aaaaactaat ttaaattaat agctatgtcg tcaaatagtg tacttagcct
    12541 gtttaaatca tccataggta aaaaagttat catggcactg acagggcttt ttttaagtat
    12601 attcctggtg gttcacgtta gtggaaattt ccagttgttt aaaacagatt ccggtttgtc
    12661 atttaaccaa tactctgtat taatgactac caacccattc attaaaacca tttcatatct
    12721 gttgtacatc acaatagttt tacatgccat aagaggaatt atgctggtgc ttgaaaacaa
    12781 aaaagcccgc cctgtatctt atgccaagta tgatggaaag gctaatagca aatgggcatc
    12841 acgaaatatg ggtttactgg gtaccgtgat tttatttttt atcgttatcc atttgaatga
    12901 tttttggtat cagtataaat tcggacatat tccatttacc atgtataaaa cagaacttac
    12961 taccggcaaa ttagttgaag taaaagaaat ggaagccggt tttaaaatgg gttcaaaaat
    13021 ggaagagatg attgttggtg atcataagta tgtaattgta aaagatttat ataaagaggt
    13081 taaagacgag tttaaggaat ggtggatggt gcttatctat gttgtgagta tggcagcaat
    13141 ttcattccat ctttatcatg gctttaaaag ttcatttcaa tctttaggac tcaatcacag
    13201 aaaatacaac ggactaatac agtcaatagg aacatgggtt tttgccatta ttattcctgc
    13261 aagttttgca gctatgcctc tatatttttt tattaaccga taatacttac tgaaaattat
    13321 gacaaacaac atacttgacg cacatattcc tgccggtgag atagaaaaaa aatggagtaa
    13381 ataccgttca acggtgccat tgattaatcc tgccaataaa agaaacctcg aaatcattgt
    13441 tataggctct ggtcttgcag gagcttctgc agcagcctcg cttgctgaat taggatataa
    13501 agtaaaagtt ttttgctttc aggattcggc acgcagagca cacagtatag ccgcgcaggg
    13561 tggtatcaat gcagctaaaa actatcagaa tgatggcgac tcaatttatc gcttatttta
    13621 tgacactgtt aagggcggtg attaccgttc gcgcgaagcc aacgtatatc gccttgccga
    13681 agtaagtgga aatattattg accaatgtgt ggcacaaggc gttccatttg cccgtgagta
    13741 tggaggattg ttaagtaaca gatcttttgg cggtgtacag gtacagcgca cattttatgc
    13801 tgccggccaa accggtcagc agttattgtt gggtgcatat agtgcattaa tgcgtcaggt
    13861 tggtatgggt gctattacaa tgtattctcg tcatgaaatg cttgatattg tgaagataga
    13921 cggaaaatgt cgcggcatca ttgcacgtaa tttaattacc ggagagttgg aaagacattt
    13981 cggacatgca gtactgatgt gcagcggtgg ttatggaaat gtgttttatc tctcgactaa
    14041 tgcaatgggc agcaacgtaa ctgcagcatg gaaagctcat aaaaatggag cttattttgc
    14101 caacccttgt ttcacgcaaa tacacccgac ctgtattcct gtctcaggcg aacatcagtc
    14161 gaaacttaca ctgatgagcg agtcgctgag aaatgacgga cgtatttggg ttccaaagaa
    14221 aaaggaagat gcagaagcta ttcgtgccgg caagctaaac cctaatgatt taaaagaaga
    14281 agacagagat tattatttgg aaagaagata tcctgcttat ggtaatttag tgccaagaga
    14341 cgttgcctca cgtgcggcaa aagaacgttg cgatgcaggc tttggtgtta atgctacagg
    14401 cgaagctgtt taccttgact ttgcttataa catgaagtat aagtatggta aaatggaggc
    14461 tactatgaat agtatagaga accctacaga agaacaacta tttgaattag gtaaagcagt
    14521 agtaaaagaa aaatacggta acctgtttga catgtataaa cagattactg gtgtagatcc
    14581 ttaccacagc ccgatgatga tttatcctgc tgtacactat acaatgggtg gattgtgggt
    14641 tgactataat ctgatgacca ccgtaccggg cctttacgca cttggtgaag ccaacttcag
    14701 cgatcatggc gccaaccgtt tgggtgcttc tgcactaatg caaggtcttg ctgatggata
    14761 ttttgtgatt ccttatacca ttggagcata tctcagcagc gaaatcagaa ccaaagccat
    14821 cagcaccgac catgaagagt ttgttgctgc agaaaaccgt gtgagagaaa cattgcaaaa
    14881 gctcatgcag attaaaggga caaaatctgt tgatcatttt catcgtcagc ttggaaaaat
    14941 aatgtgggaa aaatgtggaa tggctcgtaa tgcagttgca ctaaaagaag ctattgcaga
    15001 aatacaagag ctcagaaaac agttttgggc agatgtgcgt attccgggtg agatgaatga
    15061 gtttaaccct gaattggaga aagccggcag agtagcagat tttcttgagt tgggtgagtt
    15121 gatgtgtatg gatgcattgc aacgtaatga gtcatgtgga ggtcacttca gagaagaaca
    15181 tcagacagaa gaaggagagg ctatgcgtga cgatgataac tttgcatttg cagctgcatg
    15241 ggagttgaca ggcgattcgc aatggaaaat gaataaagaa gagttgaaat ttgaaaacat
    15301 taaaattgca caacgcagtt ataaataata ttttatcaga aaaaattaaa ataatacagg
    15361 tatgaatatc accctgaaag tttggaagca aagaaataaa gatgagagag gtcgttttga
    15421 aacatatcag gtacaggatg tgtcggaaga catgtctttt cttgaaatgt ttgatgtgtt
    15481 gaatgaacga ctaattaacg aaggaaaaga tcctgttgca tttgaccatg actgccgtga
    15541 aggaatttgt ggtgcttgca gcatgtatat taacggtcgt ccacatgggc cattgcaagg
    15601 ggtcactact tgtcagttgc acatgaggtc ttttaaagat ggtgacacta ttgttgttga
    15661 gccatggcgc tctgcagcat ttcctgtaat taaggacttg gcagttgatc gttcatcatt
    15721 cgacagaata atggctgccg gtggttttgt gtcggtgaat accggaaatg cacaggatgc
    15781 aaatagcatt ttaataccaa aagatgatgc tgacaaagct tttgaagctg cagcatgtat
    15841 aggctgtgga gcttgtgttg cagcatgtaa aaattcttct gctatgctat tcgtttcagc
    15901 aaaagtatcg cagctttcat tgttgcctca gggaaaggcc gaaaggcatg agcgtgtaat
    15961 gaaaatggtt gcacaaatgg acgatgaagg ttttggcaat tgtaccaaca caggcgcctg
    16021 cgaagcagag tgtccaaaag aaatttctat tgaaaatatt gcccgtatga acagggaata
    16081 tctttcagca aaaatttctt aaacagcatc tactgaactt tttttagcat tattcgtaaa
    16141 aacaaacgaa cgggtaattt attaccggat ggttcttttt ctaagaatca ttttttaaaa
    16201 ataaaatcgc atgacagcat cagaagaaga aagaatcaga aaagcatttt cagataaaga
    16261 ctggcaggaa attaaagctc aggatacctg gcagattttt aaaataatga gtgagtttgt
    16321 tgaagggttt gaaaaaatga gccgcatagg cccttgtgtt tcaatatttg gttcggcaag
    16381 aacaaaacca gatacaagat attatttact ggcagaagaa atagcttatc aactcacaca
    16441 atccggttat ggtgtcataa cgggaggagg cccgggtatt atggaagcag gtaacaaagg
    16501 tgcacaacgt gccggtggaa aatctgttgg tttaaatatt aaacttccgt ttgaacaaac
    16561 atcaaatccc tttgtagatc atgataagaa tctgatgttt gattacttct ttgtacgcaa
    16621 ggtaatgttt accaaatatg cccagggttt tgttgtactt cccggtgggt ttggtacgtt
    16681 agacgaattt tttgaagcgt tgacacttat tcagacaaaa aagattggca gatttccgat
    16741 tgtacttgtt ggcaaagagt attggggtaa attgtttgaa tggctgcaaa cagttatggt
    16801 tgatgaaggc aatattttac ctgaagacct ttcactcttt aaattagttg atgaagcgca
    16861 tgaagcaaag tcgcatattg atgcattcta cagtaaatat ttactgaaac ctaactttta
    16921 atccctgcgc cattcactgc aaaaaattgc agcagacatg gcaatattta aagactctac
    16981 agagctgctt ttttcatcag gaaactttgg aatggaaata tccagatcaa tcaattgctg
    17041 aattgaatgc gataaacctc ttgattcatt tccgaaaacc aaaacagcag gttttttctt
    17101 agcgacagaa taaacagact tgccattaag catggtagca taaaaagtgg cattttgttt
    17161 attggcagaa atccattttt caacatctgt ataaaaaaca ttcactctga aaatagagcc
    17221 cattgtagcc tgaattgttt tggaattata aagatcaaca gaagttgatg aacatactat
    17281 ttgcttaata ccaaaccaat ctgcaattct gataatacta cccaaattac cggggtcgct
    17341 gatgtcatca aatgccagaa tgatatcatt aaattttatt tcggtttcct gttgttgccg
    17401 catggctact aaagccaaaa ctccattagg ttgttttaat gatgatatcc gctctaattc
    17461 ttttgtttta acctgtatga gtttttcatt taatagactt aatatttcat tatttgcttg
    17521 tccaatccac tcatcagtag cataaacagc tataatatta aaagctgaat gagccagttc
    17581 tgtaaccatt ttttcgccct caacaacaaa tttttgttgc tcatgacggc ttgccttgtc
    17641 ctttaatgac tttatctgtt ttatttctga acttgtcagc attagaagaa tactgttaaa
    17701 gggtataaag atagatgatc aataagtttt atttttaccg catctgaaaa aatatgcgca
    17761 aaaggcagta cggcatttta attactctga ttttactggt agcatcttgc agccccttta
    17821 agcaattgga caaaggcgag tatttgctga atcgcaatca ggtaataata gacaagcccg
    17881 aactgcgcga aggtgttaaa gcaattatca aacaaaaagc caataggaga attttagggt
    17941 tgtttcgttt tcatcttgct gtatatagta tgggtaatcg gggtaagcca acaaaattta
    18001 aaaattggtt aaagaatacg gtaggagagg agcctgaagt tttggatacg ctgtttacca
    18061 aaaagtcaac acaacagatc aaacagttta tgtctaagaa tggctatttt gatgcagaag
    18121 ttaaagacag taccatttac agaaagaaaa aagctattgt tcgttacacc attgttgcac
    18181 ataaaccttc ttttataaaa tatgtggcaa gagagtgtgc agacgataac ctcgattctc
    18241 ttgtaataaa agatacttca acctcaaagc tgcaagcagg catcatttat aatgaaggca
    18301 tattgcaagg cgaccgcgaa cgactgacaa aaaaacttcg caacgaaggt tattttcagt
    18361 tttcaccgca gtttgtttct tatcgtgttt atacatcaga tactacaaaa aatatagccg
    18421 tatttcagga tgtagcagca ttttcaaacc ccgattcaac aataagttcc gctgcattac
    18481 cacatacaaa gtttaaattg aatcggataa ttttcagccc agactatgat ccattaagtc
    18541 ttaatacatc attaattcaa tatgatacag tagcctataa aggttatttg tttgtgaaac
    18601 gagcaggaaa gagaatgccc gtaaaaccgg aggtaatcgt taatcataca tttcttaaac
    18661 ccgaagctgt tttcaggcag agtaatgcag actataccta taaaggactt aacctgttag
    18721 gtatgtatcg ttttgtaaac atcaactata acgttgttga cagcaataga cttgattgtt
    18781 ttattaatct tggtacaacg ccaaaacaag agtataaaac agagcttgaa ggtaccaata
    18841 acggtggcaa cctaggtgtt gcaggtgatt ttacttatag aaacaaaaat gtttttagag
    18901 gtgcagagat gtttgagttt aagatcagag gtgcattaga atcgcaaaag aagtttactg
    18961 atgataaaga gaacaaagtc ttgttgttat tcaatactta tgagttaggt gttgagaatt
    19021 ccatttattt gcctaaagcc ataaaaccat tcaaccgttt atttaaaaat atagctgcca
    19081 atcctgtcac tgttttttct accaattata atatccagaa tcgtccggag tttaatcgtt
    19141 ctatcctcga tttttctgca tcattagaat ggcggaaggg taggtttatc aaacaaatag
    19201 taacaccatt tcaggtaaat tttgttaatg taaagctcga gcctgaattc aaagcacgat
    19261 tggaaagcct gaatgaccct gtgctgttca gcagttatga caaccacctt atttcaaatg
    19321 gacgttatac cctcatctat aacaatcagc aattgaatga gcctggcaat tttaaatttt
    19381 tcagaacaaa tattgaattt gccggaaata cactttactg gttcagacaa atagaaggta
    19441 cagataagga tgcgaacaac agttatacta ttcttaacaa aaggtttgct caatacttca
    19501 ggcctgatgc cgatttcaga tattatcaga atataaatgc acacaactct attgtttaca
    19561 gaattgctgc tggtattggt attgcctatg gcaactcgaa aataattcct ttcgaaaaaa
    19621 gtttttatgc cggtggatca aatgatttac gtgcatttcg tgctcgtaat gttggtccgg
    19681 gaaattttac cagtgtgaac aattttgaac gtacaggtga tataaaaatc aatggtaata
    19741 ttgaataccg tttcgatatt ttgaaaattc tgaaaggtgc attcttcctt gatgccggaa
    19801 atatttgggt tcgtaaaaaa gaatcatcac gtcccaatgc tgagtttgaa attgataagt
    19861 tttacaaaca attagctata ggtagtggct tgggtttccg ttttgatttt actttcttta
    19921 tttttcgttt ggatattggt gttcctattg ttgaccctca atacaaaggt gatgataaaa
    19981 tggttgcaga tgaagtgaaa ccacgtagtc tgatttataa ttttggaatt ggatatcctt
    20041 tttaaaggta tttaccagtc gagaaaatca tctaatgcac gtctttcttt tttagtaggt
    20101 cttccttcat tgtgttgcct gtaggtacgc acggcaagac tatgcatttt aattttttcg
    20161 tactcatcgg ctgacgtcag gtctttgcag aattgatctt taactgctgc tgctactctg
    20221 ttttctattg gttgaataac ctgaaatact aaagtaaaag aaccacgtct gatagaaatt
    20281 gtttctcctg tttttaccat tcgtgatgct ttaacagcag tttcgtcaat aaaaactttc
    20341 ccttttgcac aggcctccgc agcttgcgaa cgtgttttga atatacgaac tgcccacatc
    20401 catttatcaa tgcgcacttt ttccggttga atattcatgt tcaaaattat catcattaaa
    20461 gcacttaggg ttgctgaata cctttcatcg aaaactgaat tcttcctgtt ttactatctg
    20521 cacttaatac ttttaccatc acctgttgat ttagccgcac tgccgatgaa atatcggaaa
    20581 taaattcatc actgatttct gatatgtgaa ccagtccgtc ttttttaatt cctatatcta
    20641 caaatgctcc aaagttcgta atgttagtaa taattccggg cagcaacaag tcaggctgca
    20701 agtcattgat gctttttatt tcagtattaa actcaaaaac ttttataata cctcttacat
    20761 cccttccggg ttttgataat tcagcaatga tatctttcaa agtaggtaac ccaacttcag
    20821 cagatacata ttttgaaata tcaatcttct gaatcagact atcattgcca attaaaccgg
    20881 ctattgttgt gtcaatgtct tttgccatac gttcaacaag agtgtagttt tctgggtgaa
    20941 cagcactttt gtctaatgga ttatcaccgt taacccggat aaatcctgca cacatttcat
    21001 aggttttatc acccatgcct ttaatatttt tcaattcatt tctggaatga atacctgagt
    21061 tggctcttgc atctataatt ttcttagcca ttacttcgcc aatgcctgaa acgtaggtca
    21121 gcagtttttc acttgctgtt tccagattaa cgcctacagt gtttacgcag tgttcaatgg
    21181 tttgatctaa tgcagtgttg agcatcatct catcaatttc atgctgatat tgtccaatgc
    21241 cgagtgagcg tggatctatt tttatgagtt cggcaagcgg atccatcaac ctgcgaccaa
    21301 tagaaacagc accacggacg gttacatcat attcagggaa ctctttgcgc ccttctgctg
    21361 aagcagagta aacagaagcc ccgtcttcat tcacaacaaa tacctgaatt tttctgtcaa
    21421 atcgcaaacg ctgaatgaat gttgccgtct cacgaccggc agtgccgtca ccaatagcaa
    21481 tggcatcaat tttatattgg cttaccaacg ttgagatttt atgcgatgca attttctgtt
    21541 ggctttgcgg tgggtgagga aaaatggttt cattatgtaa aagcctgccg tttttgtcga
    21601 ggcaaacaat cttacatcca cttttaaatc cggggtcaat ggctaaaata tttttttgac
    21661 ctaatggagg tgcaagtagc aattgtttta aaacaacagc aaacaatctt gctgattctg
    21721 tttcagcttt ctgcctgtaa tgcccaatgg tttcattttc cattgaaggt acaagaagtc
    21781 tttcacatgc atcttcggca gctgttttaa cctgagtgtt taaataaaaa ccttcggttt
    21841 ttatggtatc aaatagcaga ttcagtaaaa atgttgaatc cggcattaca gaaactctta
    21901 gtaacttttc tttttcgccc cttaaaatgg ccaataagcg atgtgcagca caactactta
    21961 ttttttcctt gtaatcgaaa tagtctttgt atttaacagc ttcttcaacc ttagatttga
    22021 tgacctttga tacaataaaa gatttctgct caaagcattg acgaaacgtt ttccttacct
    22081 gatgattttc tgaaattaat tctgcaataa tatcacgagc cccttgcaat actttctccg
    22141 tgtcagaaaa ggttgatgat atatttttct ctgcaagttg ctttgctttt ctgtcatcat
    22201 gaatgagcgt ttgtgctaat ggttcaagtc cagcatctct ggcaacttgc gcacgatttt
    22261 ttcttttttc tttaaaggga agataaatat cttcaagctc atttaatgtt gtggcggctt
    22321 caattttatt ttttaatgaa tctaaaactc ctttagcctg aagtgtatta atgatgaatt
    22381 gctttctgtt gttaaagtcc tcaacttctt tgagtaactt ttttaactcc agcagatttt
    22441 catcactcag ccctccggtt ttttcttttc tatagcgagc tataaaagga attgtagcac
    22501 cctcttcgaa tagctttata gtttgttcaa gctgatgaac tgatataccg gtgctgcctc
    22561 taaacccttt tagcaataaa gaatcaacca agataacaga tgaaaaatct taaaacaaaa
    22621 agttactgag catagccatc attgatccat ttttcaatgg ttgcaatagc ttctttgctg
    22681 agttgtggtg ccatgcgagg cattgcttta acaccacttt catgctttat agacgctaaa
    22741 agtttatgtt tttcacaagc atctttcaca ccttcataga aactaaggtc aagtccatct
    22801 gctttttttg ctgggctgtg gcatttggtt ccgcaggctg catcaactat tggtttaaca
    22861 tgcactgcat agcttggttt gtcaggtaca gaaacagcag tagtggtgct tgttgtagat
    22921 tttgatgact tacaggaagg cgtaattaca gtagctgtaa caacacaaaa tgctgtggca
    22981 acggataaaa aagtgaatgt tcttttcata attattaaat ttgttttgca agtttaatag
    23041 attttcttga attgaaaaac agttagatta actcatctca tgtaatttta ccgaaaattc
    23101 agattgtgtc ctataaacca aatgattatt atgcacgtaa agcaaagcaa gaaaactttg
    23161 cagcccgaag tatttataaa ctagaagaga tagatttaaa gcataaatta ttcagacaaa
    23221 gggataatgt acttgactta ggtgcctcac ccggatcgtg gttgcaatat gcttcaaaaa
    23281 aagcggggga gaagggtaaa gtttttggta tagacctgaa gccggtagaa gtgaaattgc
    23341 ccaatgtgtt tactgttcag gataacatag aaacttttga tctgcaaacc ttgtttgacc
    23401 gctatggctt taatgataag tttgatgtag taatgagtga tatggctcca aatactacag
    23461 gaagtcgttt taccgatcag gctcgttctt ttgaccttag catgcttgcc cttcaaacag
    23521 caaaacgttt tctgcggccg ggaggtcatt ttgtttgtaa aatatttgat ggcgaagatg
    23581 caatgacctt tcgcgatgaa gtaaaatcat gttttggtga atgttttgtt ttacgaccta
    23641 aaagcacacg caacagctca aaagaaatat ttgtgactgg aaagtatttt aaaacctgat
    23701 attttagtaa gtgtattaat aagtttctgc aaatttgttt ttgcacttca aatcattagc
    23761 tatttttact caactaaaag aaatggatat gaagagaatt ttacttagtt tagcttttac
    23821 cattacaggt ttccttgcat ttgctcaatg cagcattgac agtacacaga ctgatcccgg
    23881 aatttatccc gacactttat tgcctgccac tgtgaatcaa ccttacactc aggacatcac
    23941 ctttgtgatg attactgata ccatgggcat aaccataaca aattttaaca tcgcaaatat
    24001 taccggacta ccggtaggta tgacatggca atgcaacaac tttgctaatg gatgtaatta
    24061 tgatccttct cagtctattt atggttgcgt taatttaagc ggaacaccat taattgccgg
    24121 tgtttacaat gctactgtca ccgtcattgc cacattacaa gttttaggtg atcaaactat
    24181 tagctacaca ttaccattta cagttttacc tgacactgtt agcaatccgg gcttttcaat
    24241 gaccaatagc agtgggtgtt tgccattgag tgtaacgttt gtaaccaata atcccggtca
    24301 ggcgtcatat ttgtgggatt ttggaaatgg tattcagagt acactggaaa atccaccgat
    24361 acagacatat aacgcacctg gcgattacgt agtaaca
//
LOCUS       DAOOOI010000028        24362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_382_length_24362_cov_24.980499, whole genome shotgun sequence.
ACCESSION   DAOOOI010000028 DAOOOI010000000
VERSION     DAOOOI010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 24362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24362
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_382_length_24362_cov_24.980499"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..7271
                     /locus_tag="PKX59_05355"
     CDS             <1..7271
                     /locus_tag="PKX59_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ90329.1"
                     /translation="TTCSLSNGSITLNANGGTSPYSYSINNGANFQTQNIFNLIAAGN
                     YAVVITDDNGCSVSQPIVVNDAPSPVISNVQQTNISCNGSSDGAVQMLTNGGTAPLQY
                     SIDNGLNYQALNSFSNLPAGNYNVIVQDANGCTVTTTVQLSQPSAIVLNPSATPSTCG
                     NDDGTVTVTASGGTGAFNYSIDNGLTYQPSNIFGQLYAGNYSVTVQDANGCTNTIAIA
                     VNNLSAPSIQTLTTNITCNGANNGTITVSANGGTGALSYSIDNGMSYQSGSVFQSLTP
                     GNYNVVVQDGNGCTVTSTTTITEPLAIQINSNATNTTCGNNNGSINIQGNGGTGILTY
                     SNDGGTNFQVSGQFGNVLAGTYQIVVTDGNGCTATSQVVVTDAPSPVIANMPLVQISC
                     NGLSDGVITINTLGGLAPLQYSIDNGTTTQSSGVFNNLSPGNYQIMVTDANGCTATSS
                     ATLSQPSGITANASTVNANCGTADGTITIAASGGSGTLQYSIDNGVSFQSASSFGFLV
                     AGNYSIIIRDANGCTNTVTTTVSNNSAPAISAVSTTDITCFGAGNGSISVNANGGSGT
                     LQYSINNGATFQASQIFNNVVAGTYTVIVQDAGGCTVTDVVSVSEPLMLSINNTTSGS
                     TCGLLNGQINISANGGTSPFQFSIDSGITFQSTATFSGLASGNYQLQVQDANGCTINT
                     TTLVANAPSPQISQVAHTDLTCFGSQNGTISIASSGGTSPVMYSVDGGLQFQAGAVFN
                     NLNSGSYQIMIQDANGCTATSSVVVVEPSAITVTSVTTDATCGNSNGTLTINTIGGSG
                     VLTFSIDSGLNFQSGSLFQNLLAGTYNLVVQDVNGCTSLASALVSNASAPVINATPAT
                     NVSCNGYNDGSISIQASGGLGTLTYSINGGITSFPSGIFNNLLAGSYNILVTDSTGCS
                     VTAVVNITQPAAIISSTSVVNAICGSTNGEITITASGGVGTLQYSINNGGSFQSGNIF
                     NSLGAGNYQIVVSDANGCTIGAVASVNNTNAPVIQSTNVTNVSCFGGNNGSIIINANG
                     GTGVLQYSINNVVTYSLNNTYTSLQPGNYSIQVVDAAGCIATSNVVITEPDELLFNTT
                     STPSTCGNANGQITFNVIGGTAPLQYSVNNGSSFQSGVSFTGLTATNYSVVIQDANGC
                     SASAVATITNQAGPTIATVVTNDVKCNGAANGIISINANGGTGILQYSINNGSLFQQG
                     NIFSGLSTGTYQIIIQDANGCSITAQSIINEPDAILMSVVENGSTCSQPNGGINVSAN
                     GGTGSLQYSANNGISFQTTSSFLNLPSGNYTVVVQDSNGCTAQMPATVTDAPAPVLQN
                     INVTNVLCYGNSNGSVQVQVNGGTGAINYLLSNGDVSSSGIFDSLTWGNYSVIITDAN
                     GCSVTGNAIVAQPQPLNFFVNTTASTCGNSNGVITVNSITGGTGVCVFSLDSGLTNQS
                     QNQFVNLNAGNYSVLATDSNGCSLQSNVLLANIAGPLFNALNITDALCSGAMNGSITV
                     SPQGGTGPFLYSADSGFTYQAGNIFSALSAGNYSLIIQDANGCLTDSIATIGEPLPLN
                     YQVASAVATCGSSNGTITLNATGGNGSYQYSINNGITFQSTGTFGGLIPAGYPVVVTD
                     LNGCLDSGLVSVADAPSPVLTLVSVNDATCNGFTDGVVSVTSQSGTLPIQYSINGGTW
                     QQSGSFANLSAGNYSVIVQDSYGCTDTVTALIAEPLVIALQTSTIPVICNGQSNGQTA
                     VTVTGGTMPYSYQWNAGATTASVNNLSAGTYSVVITDANNCTSSATAVVTEPVVLQMT
                     LATTPVTCFGYSNGIINTTASGGTGNLQFSIDTGATYQSSTIFSSLTFGNYNIQVTDA
                     NGCTLSVPVSMTQPSAVALTSNTVDVKCFGGNDGSLTANANGGTAPYQFVINNGNPQA
                     GTTFNNLTAGNYVISVTDAMGCLKTISDSVGQPTALSAVASGQNVLCNGQSNGAVNVI
                     ITGGVQPYMYQWTGGYTTSLVNNLPQGNYQVTVTDFNGCTIHSSASITQPTAINLGLT
                     PDPTICIGQSASVTANANGGTPPYVFKWNTGHVGATLQASPVITTTYNVFLTDSNGCN
                     SPLRYANINVNPALALVMTDDDTLCEGQSTTLTATPSGSNGGPYIINWNGQTQPATSI
                     TVAPTQTTNYVATLTDGCTLMPATANAVVVVNPLPQVVFVPLITEGCVPLIVDFQNMS
                     SNGASWQWTFGDGDKDTLINPTHTYTQPGTYSVGLYAVSNAGCENQLVLDDAIQVYEN
                     PQAAFTSNRVEASILDPWFEFRNQSIGASTYLWDFGDQRTQSTEINPMYVYGDTGTYQ
                     IIMTATSEHQCSDTAYGEVHVSGAVTVYIPNAFTPNSDGRNELFSVSGIGYTELQMII
                     FDRWGNMLFNQTSSNPGWNGMNMFNNSMCQQGVYVYKVIVKNIFNKQQEFKGTVSLLR
                     "
     gene            complement(7512..8360)
                     /locus_tag="PKX59_05360"
     CDS             complement(7512..8360)
                     /locus_tag="PKX59_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ90330.1"
                     /translation="MKKGFLFFVLGGMMLTSCVSQKKYKTQVSKYDTLKTDYDKVNYQ
                     LSACLDEQQKKSAKMSEMEKELTYLKANSTTMLKQLSDLSVLTTTQSESIRKSLENIN
                     AKDIFIQDLQREMSRKDSLNMALVMNLKGALKDVNDKDVEIKVEGSAVFISISDKMLF
                     RSGSYEITPKAKEVLGKVAEVIKAQPEVQFMVEGHTDNKSIHTAFIKDNWDLSVLRAT
                     SVVRVLQKNYGVDPSRIIAAGRSEYVSLQPNTTAEGRAMNRRTRIVILPQLDQFFKLM
                     EPKTKP"
     gene            8564..11404
                     /locus_tag="PKX59_05365"
     CDS             8564..11404
                     /locus_tag="PKX59_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90331.1"
                     /translation="MPIEKIPYFRKLKPIYTNIMNRILRLTMFFALIFGAEKATACSV
                     AGTVTSNRDSVCYQDTITLTTTGYTGTVFQWQSDNGGGWVNEAGAGATTDTYTFVPGE
                     TRQYRIIVTATGCPADTSNILTVTVGSIPVPTGTGASRCGYGQLTLTGTGSANGSLRW
                     FTAPSGGIPIGTGNSVNVTVGATTTFYLEDNTSGGGGGASPLIITEFDINDEASGSSG
                     DDLEIQNVSSLPLDVTGWRVAINNSYSDINLVNANIQTLSGIIPPGGILSWGDATTAT
                     SYWGSNILWNSGAFPTFTGWAMILDNNNVVRDFVPLNWPAANIAAMNTNVGGTPVTIP
                     PTLWTGDGVNITTVGAGMGVARTGNIDNNSNADFVIQTLDIGTTNAGMIIPFQGFGCS
                     SPRVPVVATINASTPVTINATSTALCMGQSSTLTVSSSNSNYSYTWTPAAGLNTTSGA
                     TVTATPTVPTTYYAIAVDGSCGAIDSVFIDVGPTSIAGTASVTSSTLCAGTNTTLNLN
                     GSLGSIQWQSNDGSGWVNMSGATTAPYQFAPTVTADYQAIVTSGGCPSATSNMLSVTV
                     IPVTTPTTVNDTICAGGTANLQSSGTGIVNWFTSQTGDTLVNTGNTYNVSLAATTTFY
                     VQTVDGGNVYHVGPADRSIGSQASNAPNNYGMRFDVLQEVTIDSVYIYPVTAGTVTIN
                     LVDATGANILNTKTITVPNTFNIYPVYLGFSVTGGTSYRLIVASGSVNLYYNTSGAIY
                     PYAVAGSPLSITGYYNPNPNTGAAYYYLYNWVITSGCKSGYIPVTGVVQGPPMPTITP
                     SGSLLTSSTAANYQWLLNGNIIPGANSQTYQTTQPGSYTVQVTDATTGCIVTSLPFMI
                     VSISDPNAIAAGVSIYPNPSTDKFYLNFNEAVLGNASVKLFNGVGQLIYVTEVNNVTG
                     KTVELEKDLSKGIYFMQLTTEKGTFQTKIIRE"
     gene            complement(11443..11751)
                     /locus_tag="PKX59_05370"
     CDS             complement(11443..11751)
                     /locus_tag="PKX59_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOZ90332.1"
                     /translation="MDNKNENQLNIELSEEIADGIYSNLAIITHSNSEFVVDFIKVMP
                     GVPKAKVKSRIVLTPQHAKRLMTALAENVAKYEQAHGTIKQTDGIAIPMNFGGPTAQA
                     "
     gene            11904..11977
                     /locus_tag="PKX59_05375"
     tRNA            11904..11977
                     /locus_tag="PKX59_05375"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11938..11940,aa:Asp,seq:gtc)
     gene            12316..13239
                     /locus_tag="PKX59_05380"
     CDS             12316..13239
                     /locus_tag="PKX59_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ90333.1"
                     /translation="MIDVINLKKSYGEREAVKSLNFLIKDGEFYGLLGPNGAGKTTTI
                     SIMSTILDADAGDVVIDNYNIKKNPKKVKQVIGVVPQEIALYNELSAGENLKFWGGLY
                     NVPESELKARMDELLKWFGLYDRRNDKVGSYSGGMKRRINIAAALLHRPKVLFMDEPT
                     VGIDPQSRNCIYEVIEKLHRDGMTIVYTTHYMEEAERFCDRIGIIDEGRIIAEGTLDD
                     LKKSKAIKEVISITFSKLSDTLVNELTSFWRDLKHQDDKILFYSNDIKTDLPKLVSKC
                     NQLSADIANIEIQKTNLETIFLSLTGKQLRD"
     gene            13241..14512
                     /locus_tag="PKX59_05385"
     CDS             13241..14512
                     /locus_tag="PKX59_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ90334.1"
                     /translation="MIKLAIKDLMLFIKDKRSMVLTFAIPIALITLFSFAFGGAGKNK
                     EDQKITLLLSDLDKTKLSEAAIEKLDSLKSIQIVQIQLDSAQNAIKKGNESCVLIIHK
                     GFADSLLAGNVLPLELQYDEARDIEVGLLQQSLIPTVAMLPFDLGNTQQVMANKFQKM
                     VGNSSQNSKANIQMKSDSLFSAIYQGMANNNTGGGANASDAFFDSGIKMTKLIASKND
                     NALGLIHAVAGTSVMMLLFSVVGIGVGLLDEKQEGTLKRLLYSPIDPIHILFGKLITA
                     NIISIFQLVVMFAFSGLVFGLDVSAHLPGLIIAIVATAFACSAFGVFLASFAKSRQQV
                     QGLSTLIILVMSAIGGSMMPLFFMPAFMQKLAVVSVNYWSIQSFYDIFWRNLSITSVT
                     FLSRIGVLLLIGTILNIIAVYLFRKNILKLT"
     gene            complement(14545..15648)
                     /locus_tag="PKX59_05390"
     CDS             complement(14545..15648)
                     /locus_tag="PKX59_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOZ90335.1"
                     /translation="MYLSNLSLVNFKNYTEAQLNFSSGVNCFTGSNGSGKTNIIDAVY
                     YLSFTKSYFNISDTQNIHHGESLFVIQGKFVVNEKEEDVFCGVKTGFKKQVKRGNEEY
                     ERLSDHIGLFPIVMVAPVDHILIAEGSDERRKFIDSIISQVNKPYLENLISYNKALMH
                     RNSYLKQLHGRMPDPSMMEVWDEQLVNYGSAIEKERRKFIAEFIPLFNETYNYLADHA
                     EEVSINYQTQLEQDDFRTLLKLSLQKDIALQHTSAGIHKDELVFKLGKYPLKRIASQG
                     QQKTFLMAVKIAQFEYLFRHKKTKPILLLDDIFDKLDDFRVKRLMELVSRHTFGQIFI
                     TDTHPERLKKIFEDISVNIKLFEVSQGMINEVS"
     gene            complement(15676..16800)
                     /locus_tag="PKX59_05395"
     CDS             complement(15676..16800)
                     /locus_tag="PKX59_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOZ90336.1"
                     /translation="MKYIVTLCRILVGVLFIISGFIKANDTLGFSYKLDEYFLVFNMS
                     FMSAFSVALAQFICVAEIVLGVATLVGWRMNLVTWLLMLMIVFFTFLTFYSAYFNVVK
                     DCGCFGDALKLTPWQSFGKDVVLLVLILVIFAWRNKVKPLFELKKTRWVVIFALFASS
                     LFTYYTYAHLPVIDFRPYAIGNNLKIGMLPPPNARPDSVVIVFKYKAKDGTIKEFGMN
                     NLPEDLENYEFVDRTDKVIREGDKPKIHDFTIVDADGHDHTQEFLNNPQYSFMLVAYD
                     LNKSNTKIQSKVNQLADACSKNKVQFFGLTSTVAAETDLFRHENQNMFDYYFCDGTTL
                     KTIIRSNPGLVLLQNGVVVAMWHYNDFPSWDDVNKQYLKK"
     gene            16932..17774
                     /gene="folP"
                     /locus_tag="PKX59_05400"
     CDS             16932..17774
                     /gene="folP"
                     /locus_tag="PKX59_05400"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOZ90337.1"
                     /translation="MQTFTSINCCGKLINLHKPHVMGVLNLTPDSFYDGGKYNSENAI
                     LTQVALMLNEGATFIDIGGSSSRPGSKMPTEEEEWSRIGNVIALIKQHFGDVLISVDT
                     VYSVIAKRAVDAGACMINDISAGLMDSKMIETVAALKVPYLMMHMKGRPENMQDNPVY
                     DNVLKEVIDFFSKQLLLCRKVGINDILIDPGFGFGKTVEHNFELLAKLNLLKIVGLPI
                     VAGLSRKGMIWKTLDITPSEALNGTTALNMVALMNGARILRVHDVKEAMQCIKLFNKL
                     NEVR"
     gene            complement(17790..18449)
                     /locus_tag="PKX59_05405"
     CDS             complement(17790..18449)
                     /locus_tag="PKX59_05405"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOZ90338.1"
                     /translation="MTIHREGKNWVWGTLITVLLINFLVLNFRAEHDWITVLVLIVTI
                     VFFLLILQFFRYPKRNITRNENYVIAPADGKVVVIERTEESEYYKDKRIQVSIFMSPI
                     NVHANWYPMSGKIKFLRYHKGKYLVAWHPKASTENERSTIVVEKDSNKTILLRQIAGA
                     LAKRIVYYPRENDLVKQGAEMGFIKFGSRVDIYLPLTAKINVELNQKTIGGVTVIAEL
                     A"
     gene            complement(18480..19757)
                     /locus_tag="PKX59_05410"
     CDS             complement(18480..19757)
                     /locus_tag="PKX59_05410"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOZ90339.1"
                     /translation="MADSKKAKQNHEEENPFEEMIKRLDVAAKIMKLDSEVYEIMKKP
                     SKLVYCSMPVKMDNDKTKVFEGFRVVHSTALGPSKGGVRYSTYVNENEVMALAAWMTF
                     KCAVADIPYGGAKGGITCDPSTMSKGELERLTRAYTNAMVDVFGVDKDIPAPDMNTGP
                     QEMAWIVDEYSKLKGGFTPGVVTGKPIHLGGSLGRSEATGRGVMTATMEAMAKMGLNP
                     AKCRAAVQGFGNVGSITAKHYEAKGLKIVAISDHTAAFYNPDGIDIEKAIKYRNSNKG
                     VIKGFKGGKLISNEELLTLNVDVLAPCAMENQITDENAGKIKAKLIVEGANGPTTDEA
                     DHILSKKGTVVIPDILANGGGVTVSYFEWGQNRSGLYMTEEEVNTKADHWMKQAFHNV
                     WNTSVKHKTTMRIAAYIYALGKIELGIKSRGHY"
     gene            complement(19989..21008)
                     /gene="murB"
                     /locus_tag="PKX59_05415"
     CDS             complement(19989..21008)
                     /gene="murB"
                     /locus_tag="PKX59_05415"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOZ90340.1"
                     /translation="MPMRENVSLKNLNSFGIEANARYYIDIHSEDDLILFLLQHKKTT
                     LPLLVMGGGSNMLFFKDFDGIVLKNNLHGIDVVEENEASVKIRIGAGEVWHNVVMWSV
                     EKNLGGIENLSLIPGTAGAAPIQNIGAYGVEIKNSLVAVEALAIDSAEKKVFNNHECE
                     FGYRNSVFKNAAKGKYIITAIILELKKQPQFKTEYGAIHSELEKQGIKNLSVKAISDA
                     VIAIRRSKLPDPAVIGNAGSFFKNPEITEQEFEKIKQQHPNIPHYPGAANKIKLAAGW
                     LIEQCGWKGFREGDAGCHKNQALVLVNYGNATGAEIYSLALKIKESVYNHFGVHIEPE
                     VNIIQ"
     gene            complement(21008..>24362)
                     /locus_tag="PKX59_05420"
     CDS             complement(21008..>24362)
                     /locus_tag="PKX59_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90341.1"
                     /translation="HSNNTLYAAGSFLFSGINPITRIAKWDGTQWLPLGTGCNDEIYA
                     LTGDANGNIYAGGIFTTAGGVSTERIAKWNGSTWSALGSGIGSGSNYVEALTFYNGDL
                     IAGGQFGVAGGVSVSGIARWNGTNWFDLNGGVSGLSMRVSAFKEVNGELICGGTFTAA
                     GGVSSNSVAKWNGAAWLAIPGGITGGQAKVKSLGYLFNTLYVGGNFSLAGGNAVSNIT
                     AYDGTTWADLSGGTNNQVDALLNYGNEMYVGGAFTMAGGNPATYIAKYRTTCLTNASI
                     TTTQTSCFGQCNGTATVTATGNAPFTYQWSSTPVQTDSTAADLCAGNYTVTITDNIGC
                     SITQPVTITEPAQFAVTFSSNNPTCFGLCDGNALASNNGQGTVTYNWNTIPAQTTQTA
                     TGLCEGTYSVTLIDSAGCMITDSVTIANPAPNTLTLTSSNPSCYNNCDGIAAVASTGN
                     APFQYLWNTIPAQQTDTATGLCAGVYYITVTDSTGCMATDSIEIVNPAAATLQFSTTT
                     ATCFGSCNGSASVVSSGTSPFTYLWNTTPVQTTDTATNLCAGTYFVTVTDSNLCAVTD
                     SVVITEPVANLISFTAQLPACVSSCNGLLTATSTGTAPFIYQWSNGDSISTIDSLCTG
                     LYTIIISDSIGCTVTDSISLNPAPPLPITFTTQFAGCNGVCTGAVSAAITGINPISYI
                     WSTGDTIASIDSLCQGIYTVSITDSLGCVVTDSAQIISQPVLLNPIATAPTCAGQCNG
                     LASINPSGVAPFQWQWSTGDTTSLSIDSLCAGVYYITITDSVGCVGSDSVTIVDPPAI
                     TYSASHTDATCANLCNGISTITATGNGTLTYLWNTVPPQTDSTNTNLCFGYTTYIITD
                     TNNCSVTDSVLIFEPSPIFIGNNFLGITCNGNCDGFVRALPSGGTPGYTYLWSNGVTF
                     DSIFNTCAGTYTVTVTDANLCTAVDSFTFVEPDPIVINFTVIDASCPGCTDGSIIATA
                     TGGTPPYDYLYPALGIADSAATNLGMGYYLFCAQDFNNCMQCDSVFVDEATAINSLSP
                     EIKEIKIFPNPFADKAYLSITTAESKDFKLYFFDVAGRIVNIPYNHAGNTENKQVFII
                     QNNGLKAGLYYVKIMSANEIIAIGKFIVN"
BASE COUNT         7510 a         4460 c         4670 g         7722 t
ORIGIN      
        1 atacaacatg ttctttatcc aatggtagta taactttaaa tgctaatggt ggtacttcac
       61 catattctta cagcattaac aatggtgcta attttcaaac gcaaaatatc ttcaatttaa
      121 ttgcagcagg taattatgca gtagttatta ctgatgacaa tggctgttct gtttcgcaac
      181 ctatagttgt taatgatgca ccttcacctg tgataagtaa tgttcaacag actaatattt
      241 catgtaatgg ttcaagtgat ggtgcagttc agatgctgac taatggcggc acagcacctt
      301 tgcaatattc tattgacaat ggtttaaatt atcaggcatt aaatagtttc agcaatttac
      361 ctgcaggaaa ctataacgtt attgtacaag atgcaaatgg ctgtactgtg acaacgactg
      421 ttcaactgag tcaaccttct gcaattgttt taaatccatc tgcaacacca agtacttgtg
      481 gtaatgatga cggtactgtt acagtaacag caagtggtgg aacaggagcc tttaattata
      541 gtattgacaa tggacttacc tatcaaccat caaatatttt tggtcagtta tatgccggaa
      601 attattctgt aacagtacaa gatgccaatg gttgtaccaa cacaattgca attgcagtca
      661 ataacctttc cgcaccttct attcaaactt taacaacaaa tattacctgt aatggagcta
      721 ataacggtac tatcacagta tctgcgaatg gtggaacagg tgctttgagc tactcaatag
      781 ataatggaat gagttatcag tccggttcag tttttcagtc attaactcct ggaaattaca
      841 atgttgttgt tcaggatggc aacggttgta cagttacatc aacgacaaca attaccgaac
      901 cattagccat tcaaataaat agtaatgcaa ctaatacaac ctgtggaaac aataatggct
      961 ctataaatat tcagggaaat ggtggtacag gaattttaac ttattcaaat gatggtggaa
     1021 caaactttca ggtatcagga caatttggta atgttttagc aggaacatat cagattgtag
     1081 ttacagatgg caatggttgt acggcaacat cacaagttgt tgttaccgat gcaccatcac
     1141 ctgtaattgc caacatgcct ttagtgcaga tttcctgtaa tggactaagt gatggagtaa
     1201 ttactattaa tacacttgga ggattagccc cattgcaata ttcaattgac aatggaacta
     1261 ctacacaatc atcaggtgtg tttaataatc tatcgcccgg caattatcag ataatggtta
     1321 ctgatgctaa tggttgtaca gcaacttcgt ctgctacatt aagtcaacct tcaggaatta
     1381 cagcaaacgc ttcaacagtt aatgccaatt gtggcacagc tgatggtaca ataacaattg
     1441 cagctagcgg tggcagtgga acattgcaat attcaattga taatggcgtt tcgtttcaga
     1501 gtgcatcttc ctttggattt cttgttgcgg gaaattatag cataataatt cgtgatgcaa
     1561 acggatgtac caataccgtg acaaccacag taagtaataa ttctgctcca gcaatttctg
     1621 cagtgagtac tactgatata acatgttttg gtgcaggcaa tggcagcatt tcagtaaatg
     1681 caaacggtgg ttccggcaca ttgcaatatt cgataaataa tggtgcaaca tttcaggctt
     1741 ctcaaatatt caacaacgta gttgccggaa cttacactgt gatagttcag gatgcaggtg
     1801 gttgtacagt aacagatgta gtctctgttt cagaaccatt aatgttgagt attaataata
     1861 caacaagtgg ttcgacatgc ggattattga atggtcagat aaatatttca gcaaatggcg
     1921 gcacatcacc atttcagttt tctattgata gtggaataac atttcaaagt acagcaacat
     1981 tctccggatt agcttcagga aattaccaac tgcaggttca ggatgcaaac ggttgtacaa
     2041 ttaatacaac aactcttgta gctaatgcac catcaccaca aatcagtcag gttgcacata
     2101 cagacttaac ttgttttgga agtcagaatg gtacaatatc aattgcttcc tccggtggaa
     2161 cttcacctgt catgtattca gttgatggtg gtttacagtt tcaagccggt gctgtattta
     2221 ataatttgaa ttcaggcagc tatcagataa tgatacaaga tgcgaatgga tgcacagcta
     2281 catcttcagt agttgttgtt gagccatctg caataactgt aacctcagtt actactgatg
     2341 ccacttgcgg aaacagtaat ggtaccttaa caattaatac cattggaggt tccggagtac
     2401 ttaccttcag tattgatagc ggattgaatt ttcagagtgg aagtttgttt cagaacttat
     2461 tagcaggaac atataatctt gttgtacagg atgtcaatgg ctgtacatct ttagcaagtg
     2521 cattagttag caatgcatct gcaccggtaa ttaatgcaac acctgcaaca aatgtttcat
     2581 gcaacggtta taatgatggt agcatttcaa ttcaggcaag tggtggtttg ggaacattaa
     2641 catatagtat taatggtggt attacttcat ttccaagtgg catatttaat aatctgcttg
     2701 caggaagtta taatatttta gttacagatt ctacaggttg tagtgtaact gctgttgtaa
     2761 atatcactca gccggcagct ataatttcaa gtacatctgt tgtaaatgct atttgtggat
     2821 caacaaatgg cgaaataacc attacagcaa gtggcggtgt gggtaccttg caatattcaa
     2881 ttaataatgg cggaagtttc caatcaggaa atatttttaa tagcctcggt gcggggaatt
     2941 atcagatagt agtgagtgat gcaaacggtt gtaccattgg cgcagtggcc tcagttaata
     3001 ataccaatgc acccgtaata caatcaacca atgttactaa tgttagttgc tttggaggaa
     3061 acaatggttc tatcattatt aatgctaatg gtggaacagg tgtactgcag tattcaataa
     3121 ataatgttgt tacatattcg ctaaacaata cttatacgag tttacaaccg ggtaactatt
     3181 ccattcaggt ggttgatgct gccggatgta ttgctacttc aaatgttgta attacagaac
     3241 ctgatgaatt gttgtttaat acaacttcca ctccttctac ttgcggaaat gccaacggac
     3301 aaattacatt caatgtcatt ggaggaacag caccacttca atattctgtt aataatggtt
     3361 caagttttca atcaggtgta agttttaccg gtttgacagc aacaaattat tccgttgtga
     3421 tacaagatgc caatggatgt tccgcaagtg cagttgcaac aataaccaat caggcaggtc
     3481 caacaattgc taccgttgta accaatgatg taaaatgtaa tggtgcagca aatggtatta
     3541 tttctataaa tgctaatgga ggcacaggaa ttctgcaata ttctatcaac aatggttcat
     3601 tatttcaaca aggaaatatt ttttccggat tgtcaactgg tacttatcag ataattattc
     3661 aggatgccaa tggatgcagc ataactgcac aatctataat aaatgaacct gatgctattt
     3721 taatgtctgt tgtggaaaac ggttcaactt gcagccaacc caatggtggc atcaatgttt
     3781 ctgcaaatgg aggcacagga tcgttacagt attctgctaa caatggtata tcatttcaga
     3841 caacttcttc tttcttaaat ctgccatcag gaaattatac agtagtagtt caggatagca
     3901 atggatgcac tgcgcaaatg ccggcaaccg ttactgatgc acctgcacct gtgctgcaga
     3961 atattaatgt gacaaatgtt ttatgttatg gcaatagtaa tggatcagtt caggttcagg
     4021 taaatggtgg cacaggagca ataaactatt tattaagcaa tggtgatgtt agttcgtcag
     4081 gaattttcga ttcattaacg tggggtaatt attcagtaat tattaccgat gcaaacggtt
     4141 gtagtgttac aggaaatgca attgttgcac aaccacagcc tttgaatttc tttgttaaca
     4201 caacggcatc aacctgtggt aatagcaatg gtgtaatcac agtaaatagt ataacaggag
     4261 gaacaggagt ttgtgttttc tctttagata gcggattgac caatcagtca caaaatcaat
     4321 ttgtgaattt gaatgccgga aattattctg tgctagctac agattcaaat ggttgttcat
     4381 tgcaatcgaa tgttttattg gcaaatattg caggtccttt atttaatgca ttgaatatta
     4441 cagatgcact gtgttccggt gccatgaatg gctcaataac cgttagccca caaggtggca
     4501 caggaccgtt tttgtatagt gcagatagtg gttttactta tcaggcaggt aatattttta
     4561 gcgcattatc agcaggaaat tatagtttaa ttattcagga tgccaacgga tgtttaacag
     4621 acagtattgc aacaataggt gaacctttgc ctctgaacta tcaggttgct tcggcagtgg
     4681 ctacatgtgg tagcagtaat ggaacaatca cattaaatgc cacaggtggt aatggaagtt
     4741 atcagtatag tatcaataat ggaataacat ttcagtcaac aggaactttt ggaggtttga
     4801 ttcctgcagg ttatcctgta gtggtaacag atttgaatgg atgtttggac agtggattgg
     4861 taagtgttgc agatgcgcca tcaccagtat taactttagt atctgttaat gatgcaacct
     4921 gtaacggatt tacagacggt gttgtttctg ttacatcaca gagtggtaca ttgccaattc
     4981 agtacagtat aaacggagga acttggcagc agtcaggtag ctttgctaat ttatctgcag
     5041 gaaattattc agtaatagtt caggatagtt atggctgtac agacacagtt actgcgttaa
     5101 tagcagaacc gttagtaatt gcattgcaga cttcaacaat acctgttatt tgtaatggac
     5161 aaagtaacgg acagactgca gtgacagtta ccggtggtac aatgccatat tcttatcaat
     5221 ggaatgcggg agcgaccaca gcttctgtga ataatctttc tgccggaaca tattccgttg
     5281 ttattactga tgccaacaat tgtacatcat cagcaactgc tgtagtaaca gagcctgtgg
     5341 tattacaaat gacattggct acaacaccgg taacatgttt tggctatagc aatggtatca
     5401 ttaatacaac agcatcaggg gggacaggta atctgcaatt ctctatagat acaggagcaa
     5461 cctatcagag ttcaactata ttttcatcat tgacttttgg aaattataat atacaagtta
     5521 ccgatgccaa tggctgtaca ttatctgttc cggttagtat gacacagcct tctgctgttg
     5581 ccctgacttc aaatacagtt gatgtgaaat gttttggtgg caatgatgga agtttaactg
     5641 caaatgcaaa tggaggaact gcaccttatc agtttgttat taataacggt aatccgcaag
     5701 caggtacaac atttaataat ctgacagcag gtaattatgt tatttcagtt acagacgcta
     5761 tgggttgttt gaaaacaatt tcagactctg tagggcagcc aacggcttta agtgctgttg
     5821 cttccgggca gaatgtttta tgtaacggtc agagcaatgg cgcagttaat gttatcatta
     5881 ccggaggtgt tcaaccttat atgtatcaat ggacaggagg ttacaccaca tcacttgtaa
     5941 acaatttgcc tcagggaaat tatcaggtaa cggttacaga ttttaatgga tgcactattc
     6001 attcatcggc aagtataaca caaccaactg cgataaattt aggattaacc cctgacccga
     6061 ctatttgcat tggacaatct gcatcggtga cagcaaatgc taatggtggt acgcctcctt
     6121 atgtgtttaa atggaataca ggacatgttg gtgcaacttt gcaggcttca cccgtaatta
     6181 caacaacata taatgtgttt ttaactgata gcaatggttg taattcgcct ttgcgttatg
     6241 caaatattaa tgtcaatcct gccttagctt tggtaatgac tgacgatgac accttatgcg
     6301 aaggacaaag cactacctta actgcaacac cttccggtag caatggcgga ccctatatca
     6361 tcaactggaa tgggcaaaca caacctgcaa catcaattac agttgcacca actcaaacca
     6421 ccaattatgt tgctactctg actgatggat gtactttaat gccggctaca gcaaatgcag
     6481 tagttgttgt taatccgttg ccacaagtag tttttgttcc attgataaca gagggttgcg
     6541 tgccgctgat tgttgatttt cagaatatgt cttctaatgg tgcttcatgg caatggactt
     6601 ttggcgatgg tgataaagac actttaataa atcctacgca cacctataca cagcccggaa
     6661 cttactcagt gggcctttac gcagttagta atgcaggatg cgaaaatcaa cttgttcttg
     6721 atgatgccat tcaggtttat gaaaacccgc aagctgcatt tacaagcaac agggtagaag
     6781 catcaattct tgacccatgg tttgagttca gaaatcaaag tattggggct tccacatatc
     6841 tgtgggattt tggtgatcag cgaacacaat caacagaaat caatcctatg tatgtttatg
     6901 gtgacaccgg aacctatcag atcataatga ctgccacctc tgaacatcag tgtagcgata
     6961 ctgcctatgg cgaagtgcat gttagtggtg ctgttactgt gtatattcca aatgcattta
     7021 cgccaaatag tgacgggcgt aatgaattgt tttctgtttc aggtattggc tatacagaat
     7081 tgcagatgat aatttttgac cgatggggaa atatgttgtt taatcagact tcttcaaatc
     7141 cgggatggaa tggcatgaat atgtttaata attcaatgtg tcagcaagga gtttatgttt
     7201 ataaagtgat tgtaaaaaac atattcaaca aacaacagga gtttaaagga actgtaagtt
     7261 tattgaggta aattgtaagt gaaaaaaaag ctgctctatt gagctgtctt ttttgttatt
     7321 gtctgtattg tacaatgtct ttcattatta caaatcatgc atttttgttt tgacctgacc
     7381 cttaggtttg tgcaatcata aactctcccg catttcaggg gacgttgaat ggacaaaagg
     7441 taaaaatata tgataaaaat ttagctcttc acaaattttt gcgaagggct tttgctttaa
     7501 tttaagtatt gttatggctt agtttttggc tccatcaatt taaagaactg atcgagttgt
     7561 ggcagaataa caattctggt tctgcggttc atggcacgtc cttctgcagt tgtgttaggc
     7621 tgaagactta catactcact gcgtccggct gcaatgatgc gtgatggatc aacaccataa
     7681 tttttttgta aaacacgaac tacagacgta gcacgcagta cacttaaatc ccaattgtct
     7741 tttataaatg ctgtgtgaat agatttattg tcagtatgac cttcaaccat aaactgcact
     7801 tcaggttggg ctttgattac ttctgctact tttcctaaaa cttcttttgc ctttggtgta
     7861 atttcataac ttccacttct gaaaagcatt ttatcggaaa tagaaataaa cacagcactg
     7921 ccttcaactt ttatctctac atctttatcg ttaacatctt tcaatgcgcc tttaaggttc
     7981 attactaatg ccatattcaa agaatctttt cttgacattt ctcgctgcaa gtcctgaata
     8041 aaaatatctt ttgcattaat attttcgagc gatttgcgga tactttcact ttgtgtggtt
     8101 gtcaatacag aaaggtctga tagctgtttc agcattgtag tactgttggc tttaagatac
     8161 gtcagttcct tttccatttc cgacattttt gcagattttt tttgttgctc atccaaacag
     8221 gcactaagct gataatttac cttatcataa tcagttttca aagtgtcgta tttactcacc
     8281 tgtgttttgt attttttctg actcacgcat gaagtcaaca tcattccccc caaaacaaaa
     8341 aataaaaatc cttttttcat tatttttaaa attaaaagtt aaacataaaa ttgagaatgc
     8401 aaaggtatcc aaccatatcg ctataaatgt ttaagcaagc attaatgttt ccacaaaagg
     8461 tatgttaagt aagattatca ttatttgttc acatataaaa ccatcaataa atgatttcta
     8521 aaaaagttct gcagtaaaaa tcagattaga taattttcta ctgatgccga tagaaaaaat
     8581 tccttatttt cgtaaactga aaccaattta taccaatatc atgaatagaa ttttacgatt
     8641 aacgatgttt tttgccctga tttttggagc agaaaaggca acagcatgtt ctgttgcagg
     8701 aacggtaacc tcaaaccgcg attcagtatg ttatcaggac acaattacgc taactaccac
     8761 aggctatacc ggaacggttt ttcaatggca gagtgataac ggtggtggtt gggtaaatga
     8821 agcaggtgca ggtgccacaa cagacaccta tacttttgtg cctggtgaaa ccagacaata
     8881 cagaattatt gttacagcaa ccggttgccc tgcagacact tcaaatattt taacagtaac
     8941 agtgggttca attcctgttc caacaggaac tggcgcatca cgttgcggtt atgggcaact
     9001 tactttaaca ggaacaggat ctgcaaatgg ttctttacga tggtttactg caccaagcgg
     9061 tggtattcct atcggcacag gaaattctgt gaacgttaca gtaggtgcca caactacatt
     9121 ctacttagag gacaacactt ccggtggagg tggtggtgca agtccactga taataacaga
     9181 gtttgatatt aatgatgagg cttcaggaag tagtggtgat gaccttgaga ttcaaaatgt
     9241 atcgtccctg ccacttgatg ttaccggctg gcgtgtagca attaataatt cttattcaga
     9301 cataaatctg gtaaatgcta atattcaaac attgagtgga ataattcctc cgggtggcat
     9361 tttaagttgg ggtgatgcta caactgcaac aagctactgg ggctctaata ttctttggaa
     9421 ctccggggca tttccgacat ttacaggctg ggcaatgatt cttgataaca acaatgttgt
     9481 cagagatttt gttccattaa actggcctgc agccaacata gctgcaatga atacaaacgt
     9541 tggcggaact cccgttacca ttccacccac attgtggaca ggtgatggtg taaatatcac
     9601 tacagttggt gccggaatgg gcgttgcacg taccggtaat attgataaca attccaatgc
     9661 agactttgtg attcaaacat tagatatagg aacaacaaat gcaggtatga taattccatt
     9721 ccagggcttc ggctgttcaa gcccaagagt tcctgttgta gcaacaatca atgcttctac
     9781 acctgtaacc atcaatgcaa cttcaacagc attatgtatg ggacaatctt caacattaac
     9841 ggtgagcagc agcaacagca attatagtta tacatggaca cctgctgcag gattaaacac
     9901 aacttcaggc gcaacagtaa ctgcaacacc aacagttcct actacatatt atgcaatagc
     9961 cgttgatgga agttgcggag caatagacag tgtgtttatt gatgtaggac caacatctat
    10021 tgcaggaaca gcttctgtta catcatcaac cttatgtgca gggactaaca caacattaaa
    10081 tcttaacggt tcactaggaa gtattcaatg gcaatcaaat gatggtagtg gttgggtaaa
    10141 tatgagtggt gctacaacag caccttatca gtttgcacct acagttactg ccgattatca
    10201 ggccatagtt accagcggtg gctgcccttc ggcaacttct aatatgcttt ctgttactgt
    10261 gattcctgta acaacaccaa ctactgtgaa cgatacaatt tgtgccggtg gaacagctaa
    10321 cctgcaatca agcggtacag gcattgtaaa ctggtttact tcgcaaacag gtgatacgtt
    10381 ggttaataca ggtaatacat ataatgtttc attagccgca acaactacat tttatgtgca
    10441 gactgttgac ggtggaaatg tatatcatgt aggtcctgcc gatcgtagta tcggtagcca
    10501 ggcaagcaat gctccaaata actatggaat gcgttttgat gttttgcagg aagtaacaat
    10561 tgactcagtt tatatttacc cggttacagc aggaacagtt accattaatc ttgtagatgc
    10621 taccggagca aatattctaa acaccaaaac tattacagtt cctaatactt ttaatattta
    10681 tccggtatat cttggattca gtgttaccgg tggaacatca tatagattga tagttgcctc
    10741 aggaagcgta aatctttatt acaacacatc tggtgcaatt tatccttatg cagttgcagg
    10801 tagcccatta tcaattacag gatattataa ccctaatcca aataccggtg cagcatatta
    10861 ttatctctac aattgggtta tcaccagtgg atgtaagagt ggttatatcc ctgtaacagg
    10921 agtggtgcag ggtccgccaa tgccaacaat tactccaagt ggttcacttt taacttcatc
    10981 aacagcagca aattatcagt ggttattgaa tggaaatatt attccaggag caaacagtca
    11041 gacttatcag acaacacaac cgggttctta tactgttcag gtgactgatg ctacaacagg
    11101 atgcatagta acttcattac cttttatgat tgtttctata agcgatccaa atgcaattgc
    11161 tgcaggagta agcatttatc caaacccatc aacagataaa ttttacttga attttaatga
    11221 agctgtttta ggaaatgctt cggtgaaatt atttaatgga gtaggacagt tgatttatgt
    11281 aactgaagta aataatgtaa cgggtaaaac agtagaatta gagaaagatt tatcaaaagg
    11341 aatctacttt atgcaactga ccacagaaaa agggacattt cagacaaaaa ttattcgcga
    11401 ataaaaacca aaaaagaaac cgcccttgag gcggtttctt ttttaagctt gtgctgttgg
    11461 cccaccgaaa ttcatcggta tggctatgcc atcagtttgt ttaattgtgc catgtgcctg
    11521 ctcatatttt gcaacatttt cggctaaagc tgtcatcagc cttttagcat gttgaggcgt
    11581 taaaacaatt cttgatttaa cttttgcctt aggaacacca ggcataactt taataaaatc
    11641 aaccacaaac tcggagtttg aatgggttat gatggccaga ttggaataaa taccatctgc
    11701 aatttcctca ctcagttcaa tatttaactg attctcgttt ttattgtcca ttgtctgaat
    11761 tttcttcaaa gatacggtta attcacaagc cgtcttattt aaaattcaca ggcgaagggc
    11821 tgacttgaac agaaatagca ataaaaaagt aaagattatt tgtagaaaga ataaaaaatc
    11881 tatctttgcg ccctctaaaa gagggtccgg tagttcagtt ggttagaata catgcctgtc
    11941 acgcatgggg tcgcgggttc gagtcccgtc cggaccgcct cttaaaagcc acttcaaaat
    12001 aggagtggct ttgttttttt ggataagttt cctctcagat tttggttaat gtatttcata
    12061 catttttctt aaagagaatt tcaataaatt gatattcaat gtattgtatc aggttcttct
    12121 gaccatattt aaaatcaatt tttgttgccc aaacaaagct tctgtaaagt ttaaattgct
    12181 gatagcttat ttttaatcag attattttct ttgcttaatg taaatttcag ttattgaaat
    12241 atacttttaa aaattaattg ctgatttgat actcaataaa atcagttttg ttttttctaa
    12301 tatacaatcc ctaatatgat tgatgtaatt aatttaaaga aaagttatgg cgagcgagaa
    12361 gcagtaaagt cattaaactt tttgattaaa gatggtgagt tttacggtct tttaggccct
    12421 aatggtgcag gtaaaaccac aaccatttct ataatgagta caattttaga tgctgatgcc
    12481 ggagatgttg taattgataa ctataacatc aagaaaaatc caaaaaaagt taagcaagtc
    12541 ataggtgttg tacctcagga aatagcgctc tacaatgaac tttcagccgg tgaaaattta
    12601 aaattctggg gaggtttgta taacgttcct gaaagtgagc tgaaagcaag aatggatgaa
    12661 ctgctaaaat ggtttggttt atatgatcgg agaaatgata aagtgggtag ttattcaggt
    12721 gggatgaaac gcagaataaa tattgcagca gctttgttgc atcgtcctaa agtactattt
    12781 atggatgagc caacggttgg tattgacccg caaagcagaa attgtattta cgaagtgatt
    12841 gaaaaactcc atcgtgatgg aatgactatt gtttacacaa cgcattatat ggaagaagca
    12901 gaacgtttct gcgatagaat tggaattatt gatgagggaa gaattattgc cgaaggcacg
    12961 ttagatgact taaagaagtc gaaggcaatt aaggaggtta tctcaattac tttttcaaag
    13021 ttaagtgata cacttgttaa cgaacttact tcattttgga gagacctgaa acatcaggat
    13081 gataaaattc ttttttattc aaatgatata aaaacagatt tgcccaagtt ggtttcaaag
    13141 tgtaatcaat tgagtgctga tattgccaac attgaaattc aaaaaacaaa tctcgaaacc
    13201 atctttttaa gtctaaccgg taaacaatta agagattaat atgataaagc ttgctataaa
    13261 agatttaatg ttatttatta aagacaaaag gtcaatggtg ctgacttttg ctataccaat
    13321 tgctttgata acacttttct catttgcctt tggtggtgca ggaaagaaca aagaagatca
    13381 gaaaataaca cttttactca gtgatttgga taaaacaaaa ttgtcggaag ctgcaattga
    13441 aaagttagac tcgttaaaat ctattcaaat agtacaaata caattagatt ccgcgcagaa
    13501 tgcaattaaa aaaggaaacg agagttgcgt gctaattatt cataagggtt ttgcagattc
    13561 tcttttggcc ggcaatgtat tgccattaga gttgcaatat gatgaggcca gagatattga
    13621 agtagggttg cttcagcaat cgcttatacc tactgttgca atgcttccat tcgatttggg
    13681 aaatacgcag caagttatgg ccaataagtt tcagaaaatg gttggtaatt caagtcagaa
    13741 ttcaaaagca aatattcaga tgaagtctga tagtttgttt agtgcaattt atcagggaat
    13801 ggcaaacaat aataccggtg gaggagctaa tgcaagtgat gctttttttg atagcggaat
    13861 aaagatgaca aaactcattg catcaaaaaa tgataatgca ttaggattga ttcatgcagt
    13921 tgcaggaact tctgtgatga tgcttttgtt ttcagtggtt ggaatagggg tagggctttt
    13981 agacgaaaaa caagaaggaa ctttaaagag attactttat tcacccattg accctattca
    14041 tattttattt ggaaaattaa ttactgccaa tataatttca atatttcaat tagtagtgat
    14101 gtttgcattt tcaggcttgg tatttggttt ggatgtcagt gctcacttgc cgggtttaat
    14161 aatagcaatt gttgccacgg catttgcctg ttctgcattt ggtgtttttc ttgcatcatt
    14221 cgctaaatca agacaacaag tacaaggact ttctacttta attattttag tgatgagtgc
    14281 cattggtgga agtatgatgc cgctgttttt tatgcctgca tttatgcaaa aattagccgt
    14341 tgtttcagta aattactgga gtattcagag tttttatgat attttctggc gtaacctatc
    14401 aattacaagt gttacatttt tgtcgagaat aggagtgttg ttattaatag gaactatact
    14461 caatataatt gctgtctatt tgttcaggaa aaatattctt aaactgactt gatataatga
    14521 agccataaaa tgtttacctg taaattaact tacttcatta atcattccct ggctcacttc
    14581 aaagagttta atgtttactg aaatgtcttc gaaaattttt tttaaccttt ccggatgcgt
    14641 gtcagtaata aaaatctgac caaatgtgtg acggctgact agttccatca gtctttttac
    14701 tctaaagtcg tctaacttat cgaaaatgtc atcaagcaat aaaatgggtt ttgttttttt
    14761 gtggcgaaac aaatattcaa actgcgcaat tttaactgcc atcaaaaaag ttttctgctg
    14821 tccttgcgaa gcaattcttt ttagtggata ttttccaagt ttaaaaacca attcatcttt
    14881 atgtatgccg gcagaagtgt gttgaagtgc aatatctttt tgtagagata atttcaataa
    14941 tgtgcgaaaa tcgtcttgct caagttgtgt ttgataattt attgaaactt cttcggcatg
    15001 atcggccaga taattatacg tctcattaaa caaaggaata aattcagcaa taaattttct
    15061 tctttctttt tcaatcgcac ttccgtaatt tactaactgt tcatcccaca cttccatcat
    15121 agatgggtcg ggcatgcgcc catgcaattg tttcagatag ctgtttctgt gcattagtgc
    15181 tttgttgtag ctaatcaggt tttcgagata tggtttattg acttgtgaaa tgatactgtc
    15241 aataaacttt cgcctttcat cactgccttc tgcaatcaga atatgatcaa cgggtgctac
    15301 catcacaata ggaaaaaggc cgatatgatc acttagcctt tcatattctt cattgcctct
    15361 tttaacttgt tttttaaatc cggtttttac tccgcaaaaa acatcttctt ctttttcgtt
    15421 aacaacaaat tttccctgaa tgacaaacag cgattcacca tgatggatat tttgtgtatc
    15481 agaaatatta aaatagcttt tcgtgaaaga gagataataa acagcgtcaa taatattggt
    15541 ctttccacta ccattactgc cggtaaaaca gtttacaccg gaagaaaaat ttaattgcgc
    15601 ttcagtgtag tttttaaaat ttacaagaga taggttagaa agatacactt attgcagaca
    15661 aattgattga tggaattatt ttttcagata ttgtttattt acatcatccc atgatggaaa
    15721 atcattgtaa tgccacatgg caactactac accattttgc agcagtacca atcccggatt
    15781 tgaacggatg atggtcttca atgttgttcc atcacagaaa taataatcaa acatgttttg
    15841 gttttcatga cggaataaat ctgtttctgc tgcaacggta gaggtcaatc caaaaaactg
    15901 aactttattc ttgctacagg catcagcaag ctgatttact ttactttgaa tttttgtatt
    15961 agacttattc agatcataag caacaagcat aaaggaatat tgtggattat ttaaaaattc
    16021 ctgagtatga tcatggccgt ctgcatcaac aatagtaaaa tcatgtattt taggcttatc
    16081 accttcacga attactttgt ctgtacgatc aacaaactca tagttttcca aatcttccgg
    16141 caggttgttc atgccaaatt cttttattgt accatctttc gctttgtatt tgaaaacaat
    16201 aaccacactg tccggacgtg cattgggtgg cggcaacata cctattttaa ggttatttcc
    16261 aattgcataa ggtctgaaat caattacagg taagtgtgca taggtgtagt atgtaaaaag
    16321 cgaactggca aataatgcaa agatgacaac ccaacgtgtc tttttaagtt caaacaacgg
    16381 tttaacttta ttgcgccatg cgaagattac cagaataagt actaaaagca ctacatcttt
    16441 tccaaacgat tgccatggag ttagttttaa tgcatcacca aagcaaccgc agtccttaac
    16501 aacattgaaa taagcagaat aaaaagtcag aaaggtgaaa aatacaatca tcagcatcaa
    16561 taaccaggtc actaaattca tgcgccagcc tactaaggta gcaacaccaa gaacaatttc
    16621 tgcaacacag ataaactgtg ccaatgccac agaaaatgca ctcataaacg acatgttgaa
    16681 tactaaaaag tattcatcaa gtttgtagct gaagccaaga gtgtcgttgg ccttaataaa
    16741 acctgaaatg atgaataaaa caccaactaa aattctacaa agtgtaacaa tatatttcat
    16801 actttctgtt tgttattaat gtcttttaaa tcaatacaaa taatgttgtt ttatgccaat
    16861 tgttttcaat aattgacaaa aactttttca cacctgatgt caattgatat ttttgctctg
    16921 taaatcagta tatgcaaaca tttacttcta taaactgctg tggcaaatta atcaatctgc
    16981 ataaacctca tgttatgggt gtattaaatc ttacaccgga ttctttttat gatggaggta
    17041 aatacaacag cgaaaatgca attttaacac aagtagcatt aatgctgaat gaaggcgcaa
    17101 catttattga cattggtggt tcttcttcgc gtccgggctc caaaatgccg actgaagagg
    17161 aggagtggag cagaatagga aatgtaattg cattaattaa acaacatttt ggggatgtat
    17221 tgatttctgt agatactgtt tattcagtaa ttgcaaaacg tgcagttgat gcaggtgcat
    17281 gcatgattaa tgatatttct gccggtttaa tggacagtaa aatgattgaa acagttgctg
    17341 cacttaaagt tccctatttg atgatgcaca tgaaaggccg acctgaaaat atgcaggaca
    17401 accccgttta tgataatgtg cttaaagagg tcattgattt tttttcaaag cagttattgt
    17461 tgtgcaggaa agttggcata aatgacattt taatagaccc gggttttggt tttggcaaaa
    17521 ctgtcgaaca taattttgaa ctgttagcca aacttaattt gctgaagatt gtaggacttc
    17581 caattgtagc cggtttatca agaaagggga tgatatggaa aacacttgat ataactcctt
    17641 cagaggcatt aaatggtact actgcactaa acatggttgc attaatgaat ggagccagaa
    17701 ttttaagagt acatgatgtt aaagaagcca tgcagtgcat aaagctgttt aataaattaa
    17761 atgaggtaag ataaatattg tcggaacaac taagccaatt ctgcaattac agtaacacca
    17821 cctatagttt tctgattgag ttctacatta atttttgctg tcagtggcag ataaatatcc
    17881 acacgcgagc caaatttaat gaaacccatt tcggcacctt gttttaccaa atcgttttct
    17941 cgaggataat aaacaattct ttttgcaagg gctcctgcaa tctgacgcaa taaaatagtt
    18001 ttgttggaat ctttttcaac aacaatagtg gagcgctcat tttctgttga cgctttcgga
    18061 tgccaggcaa ctaaatattt ccctttatga tagcgcaaaa attttatttt accactcatc
    18121 ggataccagt tggcatgtac atttatgggt gacataaaaa tagaaacctg aatacgttta
    18181 tccttatagt attcactttc ttcagtgcgt tcaattacta caactttacc gtcagcaggg
    18241 gcaatgacat aattttcatt acgtgtgata tttcttttcg gataacggaa aaactgaagt
    18301 atcagtaaaa agaaaactat ggttactatt aaaactaaaa ctgttatcca atcgtgttct
    18361 gcacgaaaat taagtacaag aaaattgata aggaggacag tgatgagtgt cccccatacc
    18421 caattttttc cttcacggtg aatagtcatt tttagtggat tagtaggcgg tatgttttat
    18481 taataatgac cgcgagattt aattcctaat tcgattttgc caagtgcata aatatatgca
    18541 gcaatacgca tggtagtctt atgtttcaca gaagtattcc aaacattgtg gaaagcttgt
    18601 ttcatccaat gatcagcttt tgtgtttact tcttcttctg tcatatataa tcctgaacgg
    18661 ttttgacccc attcaaaata tgatacagtt acaccaccac cgtttgcaag aatatcagga
    18721 attacaactg tacctttttt gctcagaatg tgatcggcct catcagtagt tgggccattg
    18781 gcaccttcaa caattaattt tgctttaatc tttccagcgt tctcatcagt aatctgattt
    18841 tccattgcac atggtgccag cacatctaca ttaagcgtca gtaactcttc gttagagata
    18901 agtttacctc ccttaaatcc tttaattaca cctttattag agtttctgta tttaatggct
    18961 ttttcaatat caattccatc aggattgtag aaagctgctg tatgatcaga aattgctaca
    19021 atcttcaatc cttttgcttc gtaatgtttt gccgtaattg agcccacatt tccaaagcct
    19081 tgaacggcag cacggcattt agcaggattt aatcccattt ttgccatagc ttccattgtg
    19141 gcagtcataa ctccgcgacc tgttgcttcc gaacgtccta atgagccacc aagatgtata
    19201 ggttttcctg taactacacc cggagtaaaa cctcctttta acttactgta ttcatcaaca
    19261 atccatgcca tttcctgagg gcctgtattc atatcaggtg caggaatatc cttgtccaca
    19321 ccaaacacat caaccatagc attggtatat gctcttgtca aacgctctaa ttcacctttc
    19381 gacatcgtag atgggtcgca agtaattcca ccttttgcac caccataagg aatatcagca
    19441 acagcacatt taaaggtcat ccatgcagca agtgccatca cttcgttttc attgacataa
    19501 gtgctgtatc tcacaccacc tttcgagggt cctaaagctg tgctgtgaac aactcgaaaa
    19561 ccttcaaata cttttgtttt atcattatcc attttaaccg gcatgctgca atacacaagc
    19621 ttgctgggtt ttttcattat ttcataaact tcagagtcta atttcatgat ttttgctgct
    19681 acatctaagc gcttaatcat ttcttcgaat ggattttcct cttcgtgatt ttgttttgct
    19741 tttttactat cagccatgtc ttatataata ttgattggga acgcaaaagt aggaaagatt
    19801 tatgtgtttg caaaccatgc aggaaggctg tactttttta taggttttgc atgagatata
    19861 tcagtttatc gtatgtaaat gcaagtttaa atattacttt acttactttt tagaatagta
    19921 gccaatgtca attttgacat tcagttgaca atgcactttt tatcaatgaa aaatctatct
    19981 gaaaaacact attgaatgat gtttacttca ggctcaatat gaactccgaa atggttgtaa
    20041 acagattctt taatttttaa tgcgaggcta tagatttcag cacctgtagc attaccatag
    20101 ttcaccagta ctaaagcttg atttttatga catcctgcat caccctctct aaatcccttc
    20161 cacccacatt gctcaataag ccatcctgct gccagtttta ttttgtttgc agctcccgga
    20221 taatgcggaa tatttggatg ttgttgcttg attttttcaa attcctgctc agtaatttca
    20281 ggattcttaa aaaaactacc tgcattgcca ataacagcag gatcgggtaa tttacttcgt
    20341 cttattgcta ttaccgcatc actgatggct ttaacactaa gatttttaat tccttgtttt
    20401 tcaagttccg agtgaatggc accatattca gttttaaatt gaggctgctt tttcaattcc
    20461 agaataatag ctgtgataat gtattttcct tttgctgcat ttttgaaaac actatttctg
    20521 tagccaaatt cacattcatg gttattaaac actttttttt ctgcactatc tattgctaat
    20581 gcttcaacag ctacgagtga gttttttatt tctacaccat aagcgccaat attctgaatt
    20641 ggtgcagcac ctgcagtacc cggaatcagc gaaaggtttt ctatacctcc aaggtttttt
    20701 tctaccgacc acatgacaac attgtgccat acttcacctg caccaattct tattttaacg
    20761 gaagcttcgt tctcttcaac tacgtcaatg ccgtgcaggt tgttttttaa aactatacca
    20821 tcaaaatctt taaaaaaaag catgttgctg ccaccaccca ttaccaataa tggaagtgtt
    20881 gtttttttat gctgaagaag aaaaagtatt aagtcgtcct cactgtgtat atcaatataa
    20941 tagcgtgcgt ttgcttcaat gccgaaggag tttaaattct ttaatgaaac attttcacgc
    21001 ataggcatca gttgacgatg aatttaccaa tagcaataat ttcatttgcc gacattattt
    21061 ttacataata cagtccagct tttaatccat tgttttgtat aatgaagact tgtttatttt
    21121 cagtattacc tgcatggtta tagggaatat ttactattct tcctgctaca tcaaaaaagt
    21181 ataatttaaa atcttttgat tctgcagttg taatacttaa gtaagctttg tcagcaaatg
    21241 gattcgggaa aattttaatt tcttttattt ccggtgaaag gctgtttatt gcagtagctt
    21301 cgtcaacaaa aacactatca cattgcatgc aattgttgaa gtcctgtgca caaaataaat
    21361 agtaacccat accaagattg gttgctgctg aatctgcaat tcctaatgct ggatacaaat
    21421 aatcatacgg tggtgtgcca cctgttgcag ttgctataat gctgccatct gtacaacctg
    21481 ggcaagaggc atcaattact gtaaaattga taactattgg atcaggttca acaaaagtaa
    21541 aagagtcaac agctgtacat agattggcat ctgtaacggt aactgtgtaa gtgcctgcac
    21601 aggtattaaa aatagaatcg aaagttacac cattcgacca taaataagta tatccgggag
    21661 ttccacctga tggaagagca cgaacaaaac catcacaatt tccattacag gttattccta
    21721 aaaaattatt cccgataaat attggtgaag gttcaaaaat caaaacagaa tcggtgacag
    21781 aacagttgtt agtgtctgta attatatagg tagtgtagcc aaagcacaaa ttagtgtttg
    21841 tagaatctgt ttgtggaggc acagtattcc ataaataagt cagagtgcca ttgccggttg
    21901 cagtgatggt tgaaatacca ttgcatagat tggcacaggt tgcatctgta tgcgatgcag
    21961 aataagttat tgcaggtggg tcaacaatgg taacagaatc agagcctaca caaccaacag
    22021 aatctgttat ggtgatgtaa tatacacctg cacataaact gtcaatagaa agtgatgttg
    22081 tatcgcccgt tgaccattgc cattgaaatg gtgctacgcc tgatggattt atcgaagcca
    22141 atccattgca ttgtcctgca caggttggtg ctgttgcaat tggatttagt aaaacaggct
    22201 gactgataat ttgtgcagag tctgttacaa cacatccaag actatcggta atgcttacag
    22261 tataaatacc ctgacaaagt gaatcaattg aagcaatggt atcacccgtt gaccaaatat
    22321 agcttattgg attgatgcct gtaattgctg cgcttactgc gccagtgcac acaccgttgc
    22381 atcctgcaaa ttgtgttgtg aatgtaatcg gaagaggagg agcagggttt agtgaaatag
    22441 aatctgttac tgtgcagcca atactgtcac ttatgataat ggtataaaga cctgtgcaga
    22501 gtgaatcaat agtgcttatt gaatcaccgt ttgaccattg ataaataaat ggggctgttc
    22561 cggttgatgt tgcagttaac aagccgttac aagaactgac acatgctgga agttgagctg
    22621 taaaagaaat cagattggcc acaggctctg taatcactac ggagtcggtt acagcacaca
    22681 agttactgtc ggtaacagta acaaaatagg ttcctgcgca taaatttgtt gccgtatctg
    22741 tggtttgaac aggagttgtg ttccacaaat aagtaaatgg tgaagttccg ctcgaaacaa
    22801 ctgatgcaga accgttgcaa cttccaaaac aagttgctgt tgtagtgctg aactgtaatg
    22861 ttgctgctgc aggattgaca atctcaattg aatctgtagc catacaacct gtactgtcag
    22921 taacggtaat gtagtaaaca cctgcacaaa gccctgttgc tgtatcggtt tgttgtgccg
    22981 ggattgtatt ccacaaatat tgaaaaggag catttcctgt tgatgctact gcggcaatgc
    23041 catcacagtt attataacat gaagggtttg atgatgttaa tgtaagtgtg ttaggagcag
    23101 ggttggctat agtaactgaa tctgttatca tacaacctgc tgagtcaata agcgtaactg
    23161 aataagtgcc ttcacaaaga cctgtagcag tttgtgtagt ttgtgcagga atggtattcc
    23221 agttgtatgt tacagtacct tgtccgttgt ttgatgctaa tgcattgcca tcgcacaatc
    23281 caaagcaggt agggttgttt gaagaaaagg taactgcaaa ctgtgcaggc tctgtaatcg
    23341 ttacaggttg tgtaatgctg caacctatat tatcagtaat tgtaactgta taatttcctg
    23401 cacaaagatc agctgctgtt gaatcagtct gaacaggagt agatgaccat tgataggtaa
    23461 agggtgcatt tcctgttgct gtaactgttg ctgttccatt gcactgacca aaacaagaag
    23521 tttgtgttgt agtgatggat gcattggtta aacatgttgt tctgtatttg gcaatgtagg
    23581 ttgccggatt tcctccagcc atagtgaaag cacctccaac atacatttca ttgccataat
    23641 ttaataatgc atcaacctga ttgtttgttc caccgctaag gtcagcccat gtagtaccat
    23701 cataagctgt aatatttgaa acagcatttc cgcctgcaag cgaaaaatta ccacctacat
    23761 aaagtgtatt aaataaatat ccaagtgatt ttactttagc ttgtcctccg gttatacctc
    23821 cgggaattgc taaccaagca gcaccattcc atttagcaac gctattggac gaaacgccac
    23881 ctgcagcagt aaaagttcca ccacatatca actcaccgtt tacttctttg aaagcagaca
    23941 ctcgcataga caatccacta acgccaccat tcaggtcaaa ccagttggtg ccattccatc
    24001 gtgcaatgcc gcttactgat acaccacctg ctacaccaaa ctgtccacct gcaattaaat
    24061 caccattgta aaaagtcaat gcttcaacat agttagagcc actaccaatg ccagaaccta
    24121 atgcactcca tgtactgcca ttccatttag caatacgctc agtgcttacg ccaccggctg
    24181 ttgtaaatat tccaccggca taaatgtttc cattagcatc tccggtaagt gcataaatct
    24241 catcattgca gcctgtgcct aatggtagcc attgtgtgcc atcccatttt gcaatacgtg
    24301 ttatcggatt tattcctgaa aaaagaaacg agcctgccgc ataaagtgta ttgttgctat
    24361 ga
//
LOCUS       DAOOOI010000029        24059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_388_length_24059_cov_30.878687, whole genome shotgun sequence.
ACCESSION   DAOOOI010000029 DAOOOI010000000
VERSION     DAOOOI010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 24059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24059
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_388_length_24059_cov_30.878687"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..350
                     /locus_tag="PKX59_05425"
     CDS             45..350
                     /locus_tag="PKX59_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylpyrophosphate synthetase"
                     /protein_id="HOZ90342.1"
                     /translation="MLSTESTLSERMQQLRRMGYTEDFNLKSRFAEGDKDFFKILPDD
                     FKIDLYYRFEGDSDPADEAIIYAISSERHHLKGILVNGYGVYSDPVANEILKKLKVN"
     gene            358..975
                     /locus_tag="PKX59_05430"
     CDS             358..975
                     /locus_tag="PKX59_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HOZ90343.1"
                     /translation="MELLKLIDIAGIAVFSISGVFAAMEKKLDVFGVFIIAFITALGG
                     GTLRDVLIGQLPVSWMYNLNYGLIVLLSTLASMFFSNIIGNYQKTLLTFDSLGIGLFT
                     VVGIQKGIMLDFHPAVCIALGTITACFGGVIRDILLNNIPLIFQKEVYATACILGGVV
                     YFVLMRLQMNEMITEMISITFIVVFRLVAVRFNWQLPSIYKIQKK"
     gene            1069..1464
                     /locus_tag="PKX59_05435"
     CDS             1069..1464
                     /locus_tag="PKX59_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOZ90344.1"
                     /translation="MEKVKSHIGKENYKVIIESQSGNILIADEPVENGGKNLGLSPFE
                     LIAAALSACTSITVRMYADRKDWSLTDVNTDISVNWDKANNKTIIQRKIKFSGNLSDE
                     QKNRLLQIANVCPVHKVLTNSILINTEIF"
     gene            1652..2083
                     /locus_tag="PKX59_05440"
     CDS             1652..2083
                     /locus_tag="PKX59_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOZ90345.1"
                     /translation="MSQTHFYEVNVQWKEGRIGELSSPILDKTIECATPPEFPNGVPN
                     IWSPEHLFVAAINSCYMATYLAIASNFKVELEDFSCRTVAKLEMVDGKYLITQAELFP
                     TVKLKDINDAEKAQRIADKSKAGCLVTNSMKTEIIMTTKIS"
     gene            2191..3606
                     /locus_tag="PKX59_05445"
     CDS             2191..3606
                     /locus_tag="PKX59_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ90346.1"
                     /translation="MFFQHVYDKSLAQGSYFIGCQAKGEAIVIDAQRDIDIYLEIAKQ
                     NNLKITHITETHIHADFLCGSRELAAVTGAKMYLSDEGGKDWQYEFNHEGVSDGDVIT
                     VGNLTLKVMHTPGHTPESISFLLTDHPASDKPVMVFTGDFVFVGDIGRPDLLEKAAGI
                     KGTKEIGAKQMFQSLKKFEALPEYVQVWSAHGAGSACGKALGAVHSSTVGYEKIRNWA
                     FQYGNDEQGFVDFLLADQPEPPKYFAMMKHLNKVNRPLLVEVPKHAKMSKEEFLSAYN
                     KGITVIDTRNKFEFAEGFLPGSLNIQGNNSFSTWMGWLLNYQQQFILVAGDKTIVDLT
                     RKLMRIGMDNMLGYVTDVASLGIELEKSEVINLAKFKSTINNDNYQIIDVRGLTEFEA
                     GHVEGAEHIFVGTLVQNLKMINKKKQVVIHCQAGDRSAIAFSLLHKYGFKNVWNYAGG
                     MKEWVASNEATVSERQMACAI"
     gene            3644..3961
                     /locus_tag="PKX59_05450"
     CDS             3644..3961
                     /locus_tag="PKX59_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOZ90347.1"
                     /translation="MIGLIKNMLGMENTQLTDAIKKGAFLIDVRTPAEFAGGSASGAV
                     NIPLDKIGSQLSKFKNKSNVVVFCKSGMRSMQAKNILEQNGIVNVINGGTVNDIIKIQ
                     NAK"
     gene            3951..4577
                     /locus_tag="PKX59_05455"
     CDS             3951..4577
                     /locus_tag="PKX59_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOZ90348.1"
                     /translation="MQNSNSAPVNKDSQVNDPSKQSFWNERWETKQTGWDIGYPSPAI
                     AEYFSKFSDKEVAILIPGCGNAYEAEFLVSEGFKNITLVDIAPKAVEILRKKFAGNSN
                     IRTLCADFFTIEEKFDILIEQTFFCAIPPDKRKEYVQKASEVLNPEGKIVGLLFDIMF
                     EKEGPPFGGSRVEYEQLFNPLFDLKMIPCLNSITPRAGTELFIELQKK"
     gene            4673..4900
                     /locus_tag="PKX59_05460"
     CDS             4673..4900
                     /locus_tag="PKX59_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90349.1"
                     /translation="MNKVFSGWNWMRVIRLALGVFIVIQGVHDKSLVFATLGVLFAMM
                     AVFNAGCCYAGSCQMPDDNADKSQIENSEKI"
     gene            4904..5470
                     /locus_tag="PKX59_05465"
     CDS             4904..5470
                     /locus_tag="PKX59_05465"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HOZ90350.1"
                     /translation="MQKGIIVLLLLTVVFGSCQIGQKQKLTEQQEKEYIIKGDSISDF
                     MQGILLKHVANAINHGGTEFAIGFCNMNAIPITDSVSALYHVQIQRLSDKNRNPDNAI
                     KTLEDSLAWQKIKSSSSSFIEQQSSGDIVYYKPIKTGMPTCLKCHGSSAEIESKTLAL
                     INEKYPDDKAVGYSQGDLRGMWKIKFSE"
     gene            5478..5669
                     /locus_tag="PKX59_05470"
     CDS             5478..5669
                     /locus_tag="PKX59_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOZ90351.1"
                     /translation="MKEFLRANWLIIAGTLAGAVGGYLYWKLVGCSSGTCAITSNPLN
                     STFYGALMGGLFLSLFQKS"
     gene            5836..6069
                     /locus_tag="PKX59_05475"
     CDS             5836..6069
                     /locus_tag="PKX59_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOZ90352.1"
                     /translation="MSKSVFYHAGCPVCVSAEHDIVNLIGANNVEVVHIGENRGRIAE
                     AEGKGVKSVPALVTPAGHVLHINFGASMADVKG"
     gene            6210..6473
                     /locus_tag="PKX59_05480"
     CDS             6210..6473
                     /locus_tag="PKX59_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HOZ90353.1"
                     /translation="MKVAVWDTYVTKKDGSVMHFDIIAPSEMKDTAVIYNYGKTYLKS
                     RGQEGQPLSAKECRFCHIESVRPDWEASIHKDGYFIYEMENCK"
     gene            6507..7118
                     /locus_tag="PKX59_05485"
     CDS             6507..7118
                     /locus_tag="PKX59_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOZ90354.1"
                     /translation="MSKSDFDLNFQNKSVDGRIVAALERISQAFRVLLWQESKDFSLS
                     PIQIQVIIFLLHHSDDKCKISYLADEFNMTKATISETVKTLEHKKLIQKVYEEFDNRS
                     YTIQLTKSGNAIATQTALFSKEFHKPIAKLSNDGKENMLLSLLNIINHLNKTGVITIQ
                     RMCLSCTHHALTTNGENHFCKLLNRELLSSELRIDCPEHVLST"
     gene            7240..7650
                     /gene="rnk"
                     /locus_tag="PKX59_05490"
     CDS             7240..7650
                     /gene="rnk"
                     /locus_tag="PKX59_05490"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HOZ90355.1"
                     /translation="MSKLIINRLDYARIKKSISDAKQFKSISNSEAEKLQQELDAAKI
                     VEPEAIPSNVVTMNSIVKLSFLNNDKKIQFQIVYPEQANIKENKISIFSPIATALIGY
                     KVNDEVEWIVPAGLTRIRIDEIVYQPEAAGDFDL"
     gene            7665..8933
                     /locus_tag="PKX59_05495"
     CDS             7665..8933
                     /locus_tag="PKX59_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ90356.1"
                     /translation="MKVFSYLILSFIFSIHSVECKTVRALFIGNSYTDVNNLPEIVKQ
                     IALSTGDTLIYSKNAPGGYTFLMHTTNATTISLIQAGNWDYVILQEQSQLPSFPDNDV
                     ATQVYPYAAALDSMIKASSPCATTLFYMTWGRQNGDAANCPFFPPLCTYLGMDSLLQL
                     RYTIMAQDNHAAISPVAKVWRKIRSDFPTLNLYASDGSHPNNNGSFAAACSFYSVMFG
                     KNPQLASYNFTVTASDAVTIKTVAKEVAFDSLNYWFQYDALIADFNFSTVTNTVSFFN
                     TSQNATTFNWTFGDGSSSTLMNPSHTYTASGTFNITLIASDSVCGFADTVSKTITIST
                     SGLAAPESSNSINILPNPVLDVMTFTSVKTVSSFQIFTTDGRLILTSNRPIQSNEEHS
                     LNVSMLSSGIYFLKVYSDELNASCIKFTKF"
     gene            9030..9899
                     /locus_tag="PKX59_05500"
     CDS             9030..9899
                     /locus_tag="PKX59_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HOZ90357.1"
                     /translation="MMQFIKSVFAGIGQIMLCNNIITGILFTVGIFIGNLWCGIAALL
                     AASVGTATAYLLKFSKTEIEAGLYGFSAALTGVVLIFLFENTLLIWGLVFAGAILAAL
                     LQHYFIQKCFPAYTFPFIVITWVIYFVISKFDLAQLNLQETNSFQSSTILLAGTNGFG
                     QVIFQGYFVSGILFFIAVLISSPMASCFGLAASFAGVLVALMLGYSQNDITAGIFGFN
                     AVLSAIAFAGSKKSDIAWALIVVVFTLLIHFVLISTQILSEVGGVFTFPFVAGCWITL
                     LLKYSIRNSKSIN"
     gene            complement(9969..10787)
                     /locus_tag="PKX59_05505"
     CDS             complement(9969..10787)
                     /locus_tag="PKX59_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOZ90358.1"
                     /translation="MNYIYYNLFLLFITLSNYTVSSQPNDPIPQHETFTIQSHQVGEL
                     RTINVWTPDNYKTNNDSLPVLYMPDGGIKEDFPHIANTIAKLITENKIKPLILVGIEN
                     TQRRRDLSGTTEIAKDKEIAPVVGGSEKFRAFIKDELFPEINKRYRTTSEKSIIGESL
                     AGLFIMETLFLTPEMFDNYIAFDPSLWWNNHYLVRTAKEHLSKLPPPEKRLWFAGSKA
                     KDISTYTKELAYILKKENFANIKWTYSDEPKEKHVTIFRATKEKAIIWTLNKSE"
     gene            complement(10904..11473)
                     /locus_tag="PKX59_05510"
     CDS             complement(10904..11473)
                     /locus_tag="PKX59_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOZ90359.1"
                     /translation="MAETKIKAFKNQKLWRSWLEKNQDLQEGIWLQVFKVDSGIESIK
                     ITEALDEALCFGWIDGQRKSYNEQSYLQKYTPRRAQSIWSKRNIGIVESLIKSGKMTS
                     RGFAEIEKAKADGRWARAYDSHTTMQEAEDFLKELKKHKTANAFYASLNKTNKFAINF
                     RLQTAKKPETRVKRMQEILAMLKEGKKFH"
     gene            complement(11484..11945)
                     /locus_tag="PKX59_05515"
     CDS             complement(11484..11945)
                     /locus_tag="PKX59_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007430571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOZ90360.1"
                     /translation="MSHLVDFKNVSEVGLETSPVATALAGLRANEARYFHNKYKAEFV
                     TTPVTKSKEVLKWVNTILKEERNIVIASKPLETASLMVDGIKWVFVFYESGLSINVLY
                     TLNNPKKRAVGFKLSEGMEVPAELSKFKFARQKSKLAGIIRGSFFVIKKEY"
     gene            complement(11957..12853)
                     /locus_tag="PKX59_05520"
                     /pseudo
     CDS             complement(11957..12853)
                     /locus_tag="PKX59_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
     gene            complement(13150..13485)
                     /locus_tag="PKX59_05525"
     CDS             complement(13150..13485)
                     /locus_tag="PKX59_05525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOZ90361.1"
                     /translation="MKIKQFDIWLADLNPSIGTEPGKKRPVVIVQTDLLNESHLSTLI
                     CTITTNVQKEILLLRVHLRKGQLEKLSDILVDQVRTIDNKRLIKKLGHLNKDQILKLK
                     TNLRIVLDL"
     gene            complement(13475..13762)
                     /locus_tag="PKX59_05530"
     CDS             complement(13475..13762)
                     /locus_tag="PKX59_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90362.1"
                     /translation="MSRKLAVYTFNIYLCKKRDMKTLSLKLDDKIFNETEEITSKLKL
                     ARNRYINEAVSIYNTLNKRRLLKKQLAKESKLTSKDSMDILREFEKLMDEN"
     gene            complement(13947..16301)
                     /gene="recQ"
                     /locus_tag="PKX59_05535"
     CDS             complement(13947..16301)
                     /gene="recQ"
                     /locus_tag="PKX59_05535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOZ90363.1"
                     /translation="MLAETKKKKSVKDKNEKTDTKAKVKVKEKVVEKKTKDTEKAKPV
                     AKTIVAASNKQKPTDARLKDALFKYFGFDTFKGEQEEIIQTVLRGENCFVIMPTGGGK
                     SMCYQLPALMMGGTAIVVSPLIALMKNQVDALRGFNTDEAVAHFLNSSLNKTEIAQVK
                     KDIKAGKTKLLYVAPESLTKEENVAFFKEIKVSFLAIDEAHCISEWGHDFRPEYRRLK
                     PIIEALGNIPVIALTATATEKVSLDIQKNLGMMESRLFKASFNRPNLYYEVRPKVEVL
                     KEIIKFVKNHSGKSGIIYCLSRKKVEEVASTLNVNGIKALPYHAGLDAPVRAETQDKF
                     LMEKIDVIVATIAFGMGIDKPDVRFVIHHDIPKSLEGYYQETGRAGRDGREGRCVTFY
                     SFKDIEKLEKFMKGKPVAEQEIGKQMLLETVSFAETSVCRRKYLLHYFGERYEEENCG
                     NCDNCLHPKTQIEAQDEVVLLLETVQAVKEKFAAEHIINVLIGKDDKAIKDYQHNKLE
                     VFGEGMDQDAKFWMAVVRQALLAGLLFKDIDNYGLLKLSDEGKKFMDKPHSFKITQDH
                     SYESADSDEDDEPDGGGGGAAADETLFAMLKDLCKQIAKQKNLPPFVIFQEPSLEEMA
                     IQYPINMEEMKNITGVGAGKAGKYGKPFVDLIKKYVEENEIERPQDFIVKSVVNKSGL
                     KVYLIQNIDRKISLDDMAEAKGIPLGDIITELESIVASGTKIDLNYYLNEVIDADRQQ
                     DVFDYFRTAESDSAEDALLELGENDYTIDEIRMMRVKFMSELGN"
     gene            16458..17375
                     /locus_tag="PKX59_05540"
     CDS             16458..17375
                     /locus_tag="PKX59_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010134496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOZ90364.1"
                     /translation="MESSAISGLQQYIDHDFFETVKLIFLSKGRVVCTGIGKSAIIAQ
                     KITATLNSTGTPAVFMHAADAVHGDLGVIQKDDVVICISKSGNTPEIKVLAPLLRLAG
                     NKLIAMVGDTSSYLAQQAHYILNTTVAREACPHNLAPTTSTTAQLAMGDALAVCLLEN
                     RGFSSKDFARYHPGGALGKRLYLKVGDVYKNNASPQVNPTDDLKKVIVEISGSRLGAT
                     AVVDKGKLKGIITDGDLRRMLHRSDDMLATKAKDIMTSKPKSVSPDTMAADAFILMKS
                     NNITQLVVTDKGKYLGFIHLHDLLKEGIY"
     gene            17391..18212
                     /gene="tatC"
                     /locus_tag="PKX59_05545"
     CDS             17391..18212
                     /gene="tatC"
                     /locus_tag="PKX59_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010520098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOZ90365.1"
                     /translation="MFFRRKPDPNSEMNFLDHLEALRWHLVRATLAIFVLAVVAFIYK
                     DLLFDKIIFGPKNPHFITYRLLCMLSEKWSIDLCITDIPFHLISTTMAGQFTTHMVAA
                     GIAGLILGFPYLLWEIWRFVKPALSAKEKKYTRGIVFSASFLFFCGVAFGYFILAPLS
                     INFLGSYKVSEEVQNAITLDSYISIVSIMTLACGAVFELPILVFFLTKMGIISPQFMR
                     RYRKHAFIVNLVVAALITPSPDVTSQMLVAIPLFLLYEFSIFVSVVVARQESKNE"
     gene            18224..18559
                     /locus_tag="PKX59_05550"
     CDS             18224..18559
                     /locus_tag="PKX59_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOZ90366.1"
                     /translation="MSKSVDEFNDYRAKMNKRILESDNLVLKRLYNLDANTYMDGALP
                     VQTKEMLGLVASMVLRCDDCIKYHLMQCKQNGITTEQVYEVFAVANIVGGTIVIPHTR
                     RAAEFWDEI"
     gene            complement(18663..19214)
                     /locus_tag="PKX59_05555"
     CDS             complement(18663..19214)
                     /locus_tag="PKX59_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOZ90367.1"
                     /translation="MSTKYKFQEPDAIYFVTFSVVNWIDVFTRNTYKDILLDSIRHCQ
                     KEKGLVVHAWVIMTNHVHMIISKNSLRVLPDIMRDLKKFTAYKNIGAIMESPQESRRE
                     WMFKLFKQAGENNSNNTNWQFWQQDNHPIELTDATMQEQRLNYIHENPVRAGFVYKAE
                     DYVYSSACDYGGTKGLLDLLLLE"
     gene            complement(19443..20954)
                     /locus_tag="PKX59_05560"
     CDS             complement(19443..20954)
                     /locus_tag="PKX59_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90368.1"
                     /translation="MDKKRPLLIILFLLMYAISVAQQGINNNWLMGYSSWGGQPFGQT
                     KIDFYNGTPVLSYDSLEMDFRHTHGNISDATGNMMFYTNGYYLADATNDTMQNGSGIN
                     PGAYANFASDGFAIAQAALIIQKPSSNSIYYMFHGSCDNYMMTTGCRMHQLYLTTIDM
                     NGNGGLGNVITKNVPVIIDTLNTGKIAATRHANGRDWWVMVHRVNSNQFIKLLITPTV
                     ILGPYTQNIGVTRQWDAGQAWFSQDGKKYAYYYVNGGLDIFDFDRCTATLSNAVHVTM
                     PFENGYNVGMAISPGSNALYVSNTQHVYQFDLTAANIAASQLTVATYDSFVSVFPGFP
                     SGFATLFGLSALAPDGKIYITTGNGTIHMHTIDNPDVIGLGCNVNQHSVQLQSVYFNT
                     LPNHPNYFLGCDSTLGCLCAPTVGLPTPQPPKGGVVAMTNPSNGNFTLQFNVQSTAGE
                     LEVYDVMGNLVMREYVAPWSQYKRVNIEALAKGIYLCKLKWGSVERSVKVIKE"
     gene            complement(21012..22520)
                     /locus_tag="PKX59_05565"
     CDS             complement(21012..22520)
                     /locus_tag="PKX59_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90369.1"
                     /translation="MIGKSPIQDQKNLFQPLLKEFIDMNHQLVLLSEEINWKELEADF
                     SALYSNTGLFVVAAVGGTHTELKGNDFYDHGNAFRYSVSATTADQDLQGNVWNVPTPP
                     AGFRLALNENPFYNPVFTWNDVGPPGTNTLLPHNNFQDPDDWFQPAAGTNYECATDQF
                     ACASRSFRNVATDFDYLVATDQIDNDPYTDETRWMFKKDLFKRIEENPVLQNDVVLNN
                     FYIAMQGTTLAELVPVEADKNELFVPDNFSEALLQQYRADAAQQLTLIEQQMVLLDTA
                     FVNDDTATVRILSDDIMDLQETVTATNTLAEDVIADETADRITTATAIGISNNILAAT
                     NTPEENEQIVNEIYLRTIGSDVFEFTQDDIETLYSIAIQCPMQGGNAVFMARSLYALV
                     DKNKQYDDFKICNLVGIALRKPEKAKGDTVATVYPNPANEMATLDYSLPKDSKAEFII
                     RTTTNQQILNKPLRAGDSKYSFSTADLKPSVYFYELRSNGELIANGKLIIIR"
     gene            complement(22762..23436)
                     /gene="trmB"
                     /locus_tag="PKX59_05570"
     CDS             complement(22762..23436)
                     /gene="trmB"
                     /locus_tag="PKX59_05570"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002677913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOZ90370.1"
                     /translation="MGKDKLKRFAEMQTFERVIQPEINYHSPDFELKGKWNETIFQKQ
                     QPIVLEIGCGKGEYTVALAQRYRQKNFIGIDIKGARIWRGAKTINEEKIMNAAFLRIR
                     METIEKFFAANEVSEVWITFPDPQPKESKENKRLTSPLFLKRYSTFLQPDGIVHLKTD
                     NKDLYEFTLQTVNDAGHIILHQTTDLYKELLMPVDMDIQTTYEKKYLAAKQPIFYLSF
                     RLKKEI"
     gene            complement(23484..>24059)
                     /locus_tag="PKX59_05575"
     CDS             complement(23484..>24059)
                     /locus_tag="PKX59_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmbE family protein"
                     /protein_id="HOZ90371.1"
                     /translation="GYNISVLKEENLSNDNLSNYSVIIAGVRAYNTNDWLMGYKNKLM
                     DFVSSGGTYIVQYNTNNNLGKLDNNIGPYPFKISRKRVTDEYAEMRFSEANSIVLNKP
                     NKINADDFKGWVQERGIYFAEEIDPKYQTPLSCNDKGESPLNGSLIIAQSGKGHFVYT
                     GLAFFRQLPAGVSGAYRLFSNLIALPSGQVK"
BASE COUNT         7208 a         4252 c         4469 g         8130 t
ORIGIN      
        1 tagtttgttt tagttatttt tataatcttt aaaattatgt tatcatgtta agtacggagt
       61 caactttatc agaacgtatg cagcaactaa gacgtatggg ctacaccgaa gattttaact
      121 taaaatcgcg ttttgcggaa ggggataaag atttctttaa gatacttcct gatgatttta
      181 agattgattt gtactatcgt tttgaaggcg attcagaccc ggctgatgag gcaattatat
      241 atgcgatttc atcagaaaga catcatctga aagggatttt ggtaaatggt tatggcgtgt
      301 attccgatcc tgttgcgaac gagattctta aaaaattaaa agtaaactaa atcacttttg
      361 gaactgctga aactaattga tattgccggt atagctgtgt ttagtatatc gggtgtattt
      421 gctgctatgg aaaagaaact cgatgtcttc ggtgttttta taattgcttt tataacagca
      481 ttaggtggtg gtactttgcg cgatgtatta attggacaac tacctgtatc atggatgtat
      541 aatttgaatt atggtttaat tgtgttatta agtacgcttg catcaatgtt cttttctaac
      601 atcatcggta attatcaaaa aacactgctg acattcgact cacttggaat aggtttgttt
      661 actgttgtgg gaattcaaaa gggaataatg cttgattttc atcctgctgt ctgcattgca
      721 ttgggaacaa tcactgcatg tttcggtggt gttattcgcg atattttact caacaatatt
      781 ccattaattt ttcaaaagga agtgtatgct actgcatgta ttttaggggg agttgtttat
      841 tttgttttaa tgcgattgca gatgaatgag atgattacag aaatgattag tattactttt
      901 attgttgttt tcagactggt ggctgtaagg tttaattggc aacttccatc tatttataag
      961 attcaaaaga agtaaacctg aatggctgaa ttttgaaatt gatttttttt agagttcatt
     1021 tatgttgaat cttgtttgaa taaaaagtaa tttttgaaaa cttagtttat ggaaaaagta
     1081 aaatcacata ttggaaaaga aaattataaa gttataattg aatcgcaatc agggaatatt
     1141 ctaattgctg atgagccggt agaaaacggt ggcaagaatc ttgggctttc accatttgag
     1201 ttaattgctg cagcattgag cgcatgtacc tcaataactg tgcggatgta tgccgataga
     1261 aaagattggt ctcttactga tgtaaatact gatatttctg ttaattggga taaagccaat
     1321 aacaaaacaa ttattcaacg taagattaaa ttttcgggta atctttcaga tgagcagaag
     1381 aaccgattgc ttcagattgc caatgtctgt ccagttcata aagtacttac taattccatt
     1441 ctgattaata cagagatatt ttagattaat ttgtcttctg tttatctgtt ttgaaataaa
     1501 acatgatata tctcatttaa attttagcga aaaacaaact acaatgtaac ttatgtcact
     1561 gtaaaaacag tcgggcatag gcacttttgt gctgtcattt tgaacgatta gctacacttt
     1621 aaattattca ctaaaattaa aaataacaga tatgtctcaa acacattttt acgaagtaaa
     1681 tgtacaatgg aaagagggta gaattggaga gttgtcatca cccattctcg acaaaacaat
     1741 tgaatgtgcc acaccaccgg agtttcctaa tggtgttcca aatatttggt cacccgagca
     1801 tttatttgtt gctgccatca acagttgcta tatggcaact tatttggcta ttgcttcaaa
     1861 ctttaaagta gaactggaag atttcagttg tcgcacagtt gccaaactcg aaatggtaga
     1921 tggcaagtat ttgataactc aggcagaatt atttccaacg gtaaaactga aagatataaa
     1981 tgatgctgaa aaagcacaac gcattgccga taagtcaaag gcaggttgtt tggttaccaa
     2041 ctcaatgaag acagaaatta taatgacaac aaaaatcagt taatgtaaca agagtaacaa
     2101 agtatctgaa aaatcattac ttactttgca ttatagaatt cagatacttt cagaatacaa
     2161 gcacaactat aacatttaaa ttataactat atgttttttc aacacgttta cgacaaaagt
     2221 ttagcacaag ggagctattt cataggctgc caggcaaaag gtgaagcaat tgtaattgac
     2281 gcacagcggg acattgatat ttatcttgaa attgccaaac aaaataactt aaagattact
     2341 cacattacag aaacacacat tcatgcagac tttttatgtg gctctcgtga acttgctgct
     2401 gttacaggag caaagatgta tttatctgac gaaggtggca aggactggca atatgaattc
     2461 aatcatgaag gagtaagtga tggagatgta attactgttg gcaaccttac tttaaaagtg
     2521 atgcacactc cgggccatac tccggaaagt attagctttt tgctgacaga tcatccggca
     2581 tcagacaaac ctgttatggt ttttacaggt gactttgttt ttgttgggga tattggccgt
     2641 cccgatttat tggagaaagc agcaggaata aaaggaacta aagagattgg tgccaaacaa
     2701 atgtttcaat ctttgaaaaa atttgaagca ttacccgagt atgttcaggt atggtcagca
     2761 catggtgcag gctcggcttg tggtaaggct ttaggtgccg ttcacagttc aactgtagga
     2821 tatgaaaaaa ttcgcaattg ggcatttcaa tatggtaacg atgagcaagg ttttgttgat
     2881 tttcttttag ctgaccagcc tgagccacca aagtattttg caatgatgaa acatcttaat
     2941 aaagtaaatc gtccactttt agttgaagtg cctaagcatg ctaagatgtc taaagaagaa
     3001 tttctttctg cttacaacaa aggtataacc gtaattgata ccagaaataa atttgaattc
     3061 gcagaaggct ttttacccgg aagtttaaat attcaaggga ataattcttt ctcaacctgg
     3121 atgggatggt tgcttaatta tcagcagcaa tttattttag ttgctggtga taaaactatt
     3181 gttgacttaa ccagaaaact catgcgtata ggaatggata acatgcttgg ttatgtaact
     3241 gatgttgctt cattaggtat tgaattggaa aaatcagagg taattaattt agcaaaattc
     3301 aaaagcacaa taaataacga caactatcaa atcattgatg tgcgtggttt aacagaattt
     3361 gaagcaggac atgttgaagg agctgaacac atttttgttg gaaccttagt gcaaaatctt
     3421 aagatgatta ataagaagaa gcaggtggtt attcattgtc aggcaggaga tcgttcagca
     3481 attgcttttt ctctgcttca taaatatggc tttaagaatg tgtggaatta tgccggtggt
     3541 atgaaggagt gggtagcgag caatgaagct acagtcagtg aacgacaaat ggcttgtgct
     3601 atttaatgac tttgtaaatt tctaacagtt aaattaaaat aaaatgattg gactaattaa
     3661 aaacatgttg ggtatggaaa atacacaact aacagatgca attaagaaag gagcatttct
     3721 tattgatgtg cgtactcctg cagagtttgc cggtggtagt gcatcgggcg cagtaaatat
     3781 tccgttggat aaaataggaa gtcagttgtc taagtttaaa aataagtcaa atgttgtggt
     3841 gttttgcaaa agtggaatgc gcagcatgca ggcaaagaac attcttgaac aaaatggaat
     3901 agtaaatgtt atcaatggtg gtaccgttaa tgacattatt aaaatccaga atgcaaaata
     3961 gcaactcagc gcctgtaaat aaagattctc aggttaatga tccatcaaaa cagtcctttt
     4021 ggaatgaaag atgggagact aaacaaaccg gctgggatat aggttaccct tctcctgcaa
     4081 tagcagaata ttttagtaaa ttttctgata aagaggttgc tattcttatt ccgggatgtg
     4141 gtaatgctta tgaagcagaa tttttagtga gtgaaggttt taaaaacatt accttggttg
     4201 atattgctcc aaaggctgta gaaatattaa ggaaaaagtt tgccggtaat tcaaatatca
     4261 gaactctttg tgctgatttt tttacaatag aagagaagtt cgatatttta attgaacaaa
     4321 catttttttg tgcaattcct ccggacaaac gtaaagaata tgttcaaaaa gcgtcagaag
     4381 ttttaaatcc tgaaggaaaa attgttggat tattgtttga tataatgttt gaaaaagaag
     4441 gtccgccatt tggaggaagc agagtggaat atgagcaatt gtttaatccg ttatttgatt
     4501 taaagatgat tccttgcttg aatagcatta ctccccgtgc agggacagag ctgtttatag
     4561 aattacaaaa gaagtagtta gtttatattt ttaaaattct tatgtcatgt aagtttagtg
     4621 taagaaagca ctgaagtaat atgcaattaa aaattattta atttagaatc gtatgaataa
     4681 ggttttttca ggttggaatt ggatgcgtgt aatacgttta gctttgggag ttttcattgt
     4741 cattcagggt gtgcatgaca aaagtttggt gtttgcaaca ttgggtgtat tatttgcgat
     4801 gatggcagtt tttaatgcag gctgttgtta tgcaggaagt tgtcagatgc ctgatgataa
     4861 cgctgataaa tcacaaattg aaaattcaga aaaaatataa gtaatgcaaa agggaattat
     4921 tgttttatta ctactaactg ttgtattcgg ctcctgtcaa atcggacaaa aacaaaaact
     4981 tacagagcaa caagagaagg agtatataat caaaggagac agtatatctg attttatgca
     5041 aggtatcttg ttgaagcatg ttgccaatgc gataaatcat ggtggtacgg aatttgcaat
     5101 cggcttctgc aatatgaatg ccataccaat tacagactct gtttctgcat tatatcatgt
     5161 tcaaattcag cgattgagcg ataagaacag aaatcccgat aatgcaatta aaacgttgga
     5221 agatagtctg gcatggcaaa aaataaaaag ctcttcatct agctttatcg aacaacaatc
     5281 ttccggagat atagtgtatt acaagcctat taaaaccgga atgcccactt gcttaaaatg
     5341 tcatggtagt agtgcagaaa ttgaatcaaa aactttagcg ctgataaatg aaaaataccc
     5401 ggatgacaag gcagtaggat atagccaagg tgatttaaga ggaatgtgga aaattaaatt
     5461 tagcgaataa tataagcatg aaagaatttt tacgtgcaaa ttggttaatt attgccggta
     5521 cacttgcagg agccgtagga ggatatttat attggaaact tgtaggctgc agcagcggaa
     5581 cttgtgctat tacctctaat cctttaaaca gcacatttta cggagctctt atgggagggc
     5641 tttttttatc tctatttcaa aagagctgat aatcagatct gttttaattg tcccgcacac
     5701 catagttgga attggatgaa acctgtcttt taaattaata tttccccaaa atagtaattt
     5761 tatttgtaaa agttaggaat cctaactaaa attgcagcgg taataatgcc aaataatatc
     5821 ataacatcaa acaaaatgag taaatcagta ttttatcatg caggatgccc tgtatgcgtt
     5881 agtgcagagc acgacattgt aaatttaatc ggagccaaca atgttgaagt agttcacatt
     5941 ggtgaaaatc gtggccgtat tgctgaggca gaaggtaaag gtgtaaaatc agtacctgcg
     6001 ttggtaacac ctgcaggtca tgtattgcac attaactttg gtgcatcaat ggctgatgtg
     6061 aaaggttaaa aatttgtccc acatagttag gataactacc tttgtgggac agttttatct
     6121 gaactatgaa ttttaaacaa acaatcattt taatttttgc cataagttgg gcaactttgg
     6181 caaatgcaca atcaaaaaat aaaaaattta tgaaagtagc agtatgggat acctatgtaa
     6241 caaagaagga cggaagcgta atgcattttg atatcattgc accatcagaa atgaaagaca
     6301 cagcagtgat ttataattat ggtaaaacat atctgaaatc aagagggcag gaggggcaac
     6361 cattatctgc aaaggaatgt cgcttttgcc atatagagtc tgttagaccg gattgggaag
     6421 catccataca taaagatgga tattttattt acgaaatgga aaattgtaaa taatagttta
     6481 atatttttag cttacaaatt ctaaagatga gcaagtctga ctttgatctg aattttcaaa
     6541 ataaaagcgt tgatgggcgt attgttgcag cccttgaaag aatttcacag gcatttcgag
     6601 tattactatg gcaagagagt aaagattttt ctttaagtcc aattcagatt caggttatta
     6661 tttttctgct tcatcacagt gatgataaat gcaaaataag ttatctggct gatgagttta
     6721 atatgaccaa agctacaatc agtgagactg taaaaacatt ggaacataag aaacttattc
     6781 agaaagttta tgaagagttt gataacagaa gttatactat tcagttaaca aagtcaggaa
     6841 atgccattgc cacacaaaca gctttattta gtaaagaatt tcataagcca attgcaaaac
     6901 tcagtaatga tggtaaagaa aacatgttgt tgagtcttct aaatattatc aatcatctca
     6961 acaaaacggg tgtcatcact atacaacgaa tgtgtttgtc ctgcacacat catgcattaa
     7021 caacaaatgg agaaaatcat ttctgtaaat tactaaatcg tgaactgctt tcctcagaac
     7081 tgcgtattga ctgcccggag catgttttga gcacttagtt gtattaacca tgtaaaatct
     7141 tttaagagca ttgttttttc atacacatct gctttttaaa tatttcaata gcttggaata
     7201 ccttatcttt gcaggctaat tttattataa ttatttgtta tgagtaagtt aattatcaat
     7261 cgtttggact atgcgcgtat taaaaagtct atcagcgatg ccaaacaatt taagtcaatc
     7321 agcaattccg aagcagaaaa gttacagcaa gaattagatg ctgctaaaat tgttgagcct
     7381 gaagcaatac cttctaatgt ggttaccatg aactcaattg taaaacttag ttttttaaac
     7441 aatgataaaa aaattcagtt tcagatagta tatcccgagc aagctaatat taaggagaat
     7501 aaaatttcta ttttttctcc catagcaact gcactcattg gttataaagt taatgatgaa
     7561 gttgaatgga tagttcctgc cggattaaca agaatacgca tagatgaaat cgtgtatcag
     7621 ccggaagctg ccggtgattt tgatctttaa aattacctga atccatgaaa gtattttcat
     7681 atttaatttt atcttttata ttttctatac attctgttga atgtaaaact gtaagagcat
     7741 tatttattgg caacagttat actgatgtaa ataacttacc tgaaattgta aaacagatag
     7801 cactttctac cggtgacaca ctgatatata gtaagaatgc acccggagga tatacttttc
     7861 taatgcatac taccaatgca accacaatta gcttaattca ggcaggtaat tgggactatg
     7921 ttattcttca ggaacaaagt cagctgccct catttcctga taatgatgta gcaacgcagg
     7981 tttatcccta tgcagcagca ttagacagta tgataaaagc aagtagccct tgtgcaacta
     8041 cactttttta tatgacctgg ggaagacaaa atggtgacgc tgccaattgc ccgttctttc
     8101 caccactatg tacttatttg ggtatggata gtttgttaca gttgagatat accatcatgg
     8161 cgcaagataa ccatgcagcc atttcaccgg tagcaaaagt atggcgcaaa atacgatcag
     8221 attttccaac gctaaaccta tatgcaagtg atggcagtca tccaaacaat aatggatctt
     8281 ttgctgcagc ttgcagtttc tactcagtta tgttcggaaa gaatcctcaa ttagcgtcat
     8341 ataattttac agttacagca tcagatgcgg tcacaattaa aacagtggca aaagaagtag
     8401 catttgatag cttaaattat tggtttcaat acgatgcatt gattgcagac tttaatttct
     8461 caactgttac caatacagtt agttttttca atacttcgca aaatgctact acctttaatt
     8521 ggacatttgg tgatggcagt agcagcacat tgatgaaccc atcacatact tacactgcaa
     8581 gtggcacttt taatattaca ttgattgctt ctgattctgt ttgtggtttt gcagacactg
     8641 tttcaaaaac aattacaatt tcaacttccg gtttagcagc acctgaatct tcaaatagca
     8701 ttaatatttt gcctaaccct gttttggatg tcatgacttt tacatcagtt aaaacagttt
     8761 catcattcca aatttttaca actgatggaa ggcttatatt aacttctaat agaccaattc
     8821 aatctaatga ggagcattca ctaaatgttt ctatgctatc gtcaggtatt tattttttga
     8881 aggtttacag tgatgaatta aatgcatcat gtatcaagtt tacaaaattt tagacaagaa
     8941 aattactata gaggaatttg caagttattt taataatgaa ttttctggta ttggatttaa
     9001 tttgtaaaat ctgaatttct aaccaagcaa tgatgcaatt tattaaatcg gtttttgcag
     9061 gcattggtca gattatgctt tgcaataaca tcattaccgg catactcttt acagttggaa
     9121 tttttattgg taacttgtgg tgtggcattg ctgctttgtt agctgcaagt gttggcacag
     9181 ccactgcata tttattaaaa tttagcaaga ctgaaatcga agcggggttg tatggattta
     9241 gtgcagcttt aacaggtgtt gttttgatct ttttgtttga gaacacattg cttatttggg
     9301 gattggtatt tgctggtgca attttagctg cattattaca gcattatttt attcaaaaat
     9361 gttttcctgc ttacacgttt ccatttattg ttattacatg ggtaatttat tttgtgatta
     9421 gcaaatttga tttagctcag ttaaatttac aagagacaaa ttcttttcaa tcatcaacaa
     9481 ttttacttgc gggcaccaat ggctttgggc aggtgatttt tcagggatat tttgtttcag
     9541 gaatattatt ttttatagct gtattaattt cttctccgat ggcatcatgt tttggacttg
     9601 ccgcatcatt tgctggtgta ttagtagctc tcatgttagg ctattcacaa aacgatatta
     9661 ctgcaggaat ttttggattc aatgctgtgc tttctgcaat agcttttgca ggcagtaaga
     9721 agtctgatat tgcatgggca ttaattgtag ttgtttttac cctgctgatt cattttgttt
     9781 taatcagtac acaaatacta agcgaagtag gaggggtgtt tacttttcct tttgttgcgg
     9841 gctgctggat tacattgcta ttaaagtata gcatcagaaa ttctaagagt ataaattaat
     9901 tcaacaaggt actaacaaag tatttttaca cgtaatgttt tgtgtgagca atgatgttac
     9961 atttctaatt attccgattt gtttaatgtc cagattattg ccttttcctt agttgctctg
    10021 aaaattgtta cgtgcttttc ttttggctca tcagaataag tccatttaat attggcaaag
    10081 ttttcttttt tcagaatgta tgccaactct ttagtataag tcgaaatatc ttttgctttt
    10141 gaaccggcaa accacagtcg tttttccggt ggtggtagtt ttgataaatg ttcttttgct
    10201 gttcttacca aatagtgatt gttccaccat aaagatgggt caaatgcaat gtagttatca
    10261 aacatttctg gtgtcaaaaa aagagtttcc ataataaaaa gccctgctaa agattcgcct
    10321 attatgcttt tttcagatgt agttctatag cgcttgttta tttcagggaa tagttcatct
    10381 tttataaaag ccctgaattt ttctgaaccg ccaacaaccg gtgcaatttc tttgtctttt
    10441 gcaatttctg tagtgcctga taagtctctt cttcgttgtg tgttttcgat gcctacaagt
    10501 atcaatggtt taattttatt ttctgttatt agcttagcta tcgtatttgc aatgtgtggg
    10561 aagtcttcct ttattcctcc atcgggcata tacaatacag gcaatgagtc attgtttgtt
    10621 ttgtagttat ccggtgtcca gacattgata gttcttaatt ctcctacctg atgagattga
    10681 atagtgaaag tttcgtgttg tggtatcggg tcgtttggct gtgaagatac tgtgtagttt
    10741 gaaagtgtga taaagagtag aaataaattg taatatatgt aattcatatc tttctgttgt
    10801 tactgttttg ttttaatgta ttgataaata ggtaggaatt ttaatcaatg gttaaatcac
    10861 actcagtgag ctatgcaact taaagtaaag aggtttaaaa aatttaatga aacttctttc
    10921 cttccttcaa cattgccaaa atttcttgca ttcgtttaac tcttgtttcg ggctttttgg
    10981 ctgtttgtaa cctaaagttg atagcaaatt tattagtctt gttcaacgat gcataaaagg
    11041 catttgccgt tttatgcttt ttcaattctt tcaaaaaatc ttctgcctct tgcatggtag
    11101 tatgcgaatc gtatgctctt gcccaacgcc catctgcctt agctttttct atttcggcaa
    11161 aacctcttga ggtcattttt cctgatttaa tcaaactttc aactatacca atgttgcgtt
    11221 tagaccaaat actttgtgca cgccttggcg tatatttctg caggtatgat tgctcattat
    11281 aactttttct ttgtccatca atccaaccaa agcaaagtgc ttcgtctaaa gcctctgtaa
    11341 ttttaattga ttcaattcct gaatcaactt taaatacctg aagccatatt ccttcttgta
    11401 agtcttggtt tttttctaac cagcttcgcc aaagtttttg gtttttaaag gcttttattt
    11461 ttgtttctgc catcaaattt aaattaatat tcttttttaa taacaaagaa agagcctcta
    11521 ataataccag ccaatttaga tttttgtctt gcgaatttga atttactcaa ctctgccggc
    11581 acttccattc cttcggaaag tttgaaacct acagcacgtt tctttgggtt atttagagtg
    11641 taaagaacgt ttattgacaa accactttcg taaaaaacaa atacccattt aatgccatca
    11701 accattagcg atgcagtttc taaaggtttt gaagcaatca caatatttct ttcttctttt
    11761 aaaatagtgt ttacccattt taaaacttct ttgcttttag taacaggtgt tgttacaaat
    11821 tctgctttgt atttgttatg aaaataacgg gcctcattgg cacgcaaacc tgcaagggca
    11881 gttgcaacag gcgaagtttc taatcctact tcgcttacat ttttaaaatc tactaaatga
    11941 ctcattgcaa atttatttat aaatgttttg tttcaggtag tgaaacaaat tctttatcat
    12001 cactcggtat ttttggaaaa acttcagtgt tttggttttt ccagttttct ttagcctttt
    12061 ccaaaattga tttatctgag tttacaaaat tccaaaacat ttatcgttcc tcagcaaaag
    12121 gctcaccccc gaaaagataa agtgtggttt ctccattggt ttgaaattcg cataacgaag
    12181 cattttttgc aataagtaat tgtttgcttc cataaatgtt attttcaata gttactgttc
    12241 cgtctaagac atacattcca acttcgccaa ataatttact tccaacatta attttctctg
    12301 ctgtttttgt ttttatctca atgaaatata acggactgtg aacaagaaca ggtgatttta
    12361 tcccgtttat ttcacctgca atcaatttat atgaaacatt attttcaatc catttaggaa
    12421 ttgcttcttt gctgaaatga aaaaaggtag gattagtttg ctctaaatgt tttggcaacc
    12481 caatccaaat ttggaaacca tgcaagtatt tatcgttctt tcttaaatat tccggagttc
    12541 tttcagaatg aaccacacct tttcctgcag tcatccaatt tacttccccg ggttttattt
    12601 ctatagcatt tcctatactg tctctatgga atattgctcc atcaaaaaga taagtaagtg
    12661 tagaaagccc gatatgtgga tgtgggggta catccagatt ttgatgttct tgtaaacaag
    12721 ccggacccat gtgatctata aaaacaaaag ggccaacctt tcttttttga cggaatggta
    12781 atagtcggcc aaccataaaa ttgccaatat ctgccgctct ttcttctatt actaaaccta
    12841 cgtttgattg catattacac gaataatagt tttgataata ttttgttcat gatactatca
    12901 aaattaaaac aattcatttg ctcaaaaata aaaagaaaag tgattaagaa agataatttg
    12961 aagatgggta aagttttgaa tgttagaaag tttgcgagtt tagaaaattt gaaagttgtt
    13021 ttttccttag agtattccga tgttgaatcg ttgagacagt atttaggata gcaacacgat
    13081 aaggaaagtt aataaaaatg acctgttgct cttcacacaa tgcagtgact tcgcacttac
    13141 aggttgcaac tataagtcaa gaacaattct taagttagtt ttaagtttta gtatttggtc
    13201 tttgttcaga tgaccaagtt ttttaattaa ccttttattg tctattgttc taacctgatc
    13261 taccaatatg tcacttaatt tttcgagttg tccttttctt aaatgaactc ttaataacag
    13321 aatttctttt tgtacattcg ttgtaattgt acaaattaat gttgatagat gtgactcatt
    13381 taaaaggtca gtttgaacaa taaccacagg tctttttttg ccgggctcgg ttccgatact
    13441 tggattcagg tcagctaacc aaatatcaaa ttgtttaatt ttcatccatt aacttttcaa
    13501 attcacgtaa tatgtccatg gagtctttac tcgtcaattt tgattctttg gcaagttgtt
    13561 tttttaataa acgccttttg tttaatgtat tgtaaatact aacagcctca ttgatataac
    13621 gatttcgggc caattttaat tttgaagtaa tttcttcagt ttcattaaat attttatcgt
    13681 caagctttaa tgataaagtt ttcatatctc tttttttaca aaggtatata ttaaaagtat
    13741 atactgcaag ttttcttgac atcacctctg atagtagttt gtttggaagc tatagctccg
    13801 atcactatta ttcatttacc tcaaaaataa atgattcgga aaaatataat tgataattgt
    13861 ggcttgaaat gctcactttt ataaaactca caaaaaaagg ctgcccttgc gaggcagcct
    13921 tttgtgaacg atagatttgg ttttaatcaa tttcctaatt cactcataaa ttttacgcgc
    13981 atcattctta tttcgtcaat ggtgtagtcg ttttcgccaa gttcgagcaa ggcatcttct
    14041 gcactgtcac tttctgccgt gcggaaatag tcgaacacat cttgctgacg gtcggcatca
    14101 ataacttcgt tgaggtaata attcaaatca atttttgttc ctgatgccac gatgctttct
    14161 aactctgtga tgatatcgcc taaagggatg cctttagctt cggccatatc atcgagagaa
    14221 attttgcggt cgatgttttg aataagataa accttgagac ctgatttgtt gacaacagat
    14281 tttacaatga aatcttgcgg acgttcaatt tcgttttctt ccacatattt ttttatgagg
    14341 tcaacgaatg gttttccata cttgcctgcc ttacctgcac ccacaccggt aatgtttttc
    14401 atttcctcca tgttgatggg gtactgtatg gccatttctt cgagcgaagg ctcttggaat
    14461 attacaaaag gaggcagatt tttttgtttg gctatttgtt tgcacaagtc tttgagcata
    14521 gcaaacagtg tttcgtctgc cgctgcacct cctccgccac catcgggctc atcgtcctca
    14581 tcgctgtcgg cactttcgta gctgtggtct tgtgttattt tgaaactgtg gggcttgtcc
    14641 ataaattttt taccctcatc actcaactta agcaatccat agttatcaat gtctttgaag
    14701 agcagtcctg caagcaatgc ctgacgcaca acggccatcc agaatttcgc atcctgatcc
    14761 attccttcgc caaagacttc taacttatta tgctgatagt cttttatggc tttgtcatct
    14821 tttccgatga gaacattgat aatgtgctcg gcagcaaatt tttcttttac agcctgaaca
    14881 gtttcgagca gcaatactac ttcgtcttgt gcttcgattt gtgtttttgg atgcaggcag
    14941 ttgtcgcagt tgccacagtt ttcttcttca tagcgctctc caaaataatg gagcagatat
    15001 tttctgcggc agactgatgt ttcggcaaag gaaacggttt caagcagcat ttgttttcct
    15061 atttcctgct cggcaacggg cttgcctttc ataaattttt cgagcttctc tatgtcttta
    15121 aaagaataga aagttacgca gcgtccttcg cgtccatcgc gtccggcacg gcctgtttcc
    15181 tgataatatc cttcaagaga ttttggtatg tcgtggtgaa tgacaaaacg cacatcgggc
    15241 ttgtcaattc ccattccgaa tgcaatggtg gcaacaatga catcaatttt ttccatcaga
    15301 aatttgtcct gcgtttcggc acgcacaggt gcatctaatc ctgcatggta gggcagggct
    15361 ttgatgccat ttacattgag tgtgctggca acttcttcta ctttttttct gctgaggcaa
    15421 tagatgattc ctgattttcc tgaatggttt tttacaaatt tgatgatctc tttaagtact
    15481 tcaacttttg gtcgtacttc ataataaaga ttaggcctgt tgaaggatgc tttgaacaac
    15541 ctcgactcca tcatgcctaa gttcttttgt atatcaagag aaactttttc tgttgctgtt
    15601 gcagtaagtg caataacagg aatgttgccg agtgcttcaa tgataggttt taatctacgg
    15661 tattcaggac ggaaatcgtg tccccattct gaaatacagt gtgcttcatc aatggctaaa
    15721 aaagaaactt ttatctcttt aaaaaatgca acattctctt ctttggttaa tgattcgggc
    15781 gcaacatata gcaactttgt tttgccggcc tttatatctt tctttacctg agcaatttct
    15841 gttttgttga gggatgagtt taagaagtgt gccacagcct cgtctgtgtt aaatcctcta
    15901 agtgcatcta cctgattttt catcaatgca attaatggcg aaactactat ggcagtgccg
    15961 cccatcatca aagcaggcag ttgatagcac atagatttgc caccacctgt aggcataatt
    16021 acaaagcagt tttcgccacg caatactgtt tgaataattt cttcttgctc gcccttaaag
    16081 gtgtcgaacc caaaatattt gaaaagtgca tccttcagtc gagcatctgt aggcttttgc
    16141 ttgttagagg cagcaactat agtttttgca accggtttgg ctttttcggt gtccttagtt
    16201 ttcttttcaa ctactttttc tttcaccttc actttagcct tggtgtcggt tttttcattc
    16261 ttgtctttca cagatttttt cttctttgtt tctgctaaca ttttatttat tataccaatt
    16321 aaaaatttga gttaactaaa atagatattt ttgttcaata atattgcaac aaataatttt
    16381 tttcaaaatt aaatcagaaa tacttcagat tgaaatcagt tcaacaaata attaaaattg
    16441 ccaaaaagac gcttcagatg gagtcttcgg ctattagtgg cttgcaacaa tatattgacc
    16501 atgatttttt tgagaccgta aaattaatat ttttatcgaa aggtagggta gtttgtacag
    16561 gaattggtaa aagtgccata atagctcaaa aaataacagc cacactcaac agtaccggaa
    16621 caccagccgt gtttatgcac gctgccgatg ctgtgcatgg cgaccttggg gtaatacaga
    16681 aggacgatgt ggtgatttgt atttccaaaa gtggcaacac tccggaaata aaggtcctgg
    16741 cacctttgct aaggttggct ggcaataaac taatcgccat ggttggcgac acttcttcat
    16801 atctggcgca acaggcacat tatatactta acactaccgt tgcccgcgaa gcttgtccgc
    16861 ataacttagc tcccacaaca agcactacag cacaattggc catgggtgat gccttggctg
    16921 tgtgtctgct cgaaaacagg gggttctcaa gtaaagattt tgcacgttat catccgggtg
    16981 gggcattagg aaaaagattg tatctgaagg ttggcgatgt ttataaaaat aatgccagtc
    17041 cgcaggtaaa tcccaccgat gaccttaaaa aggtgattgt agaaatatcg ggaagccgcc
    17101 ttggggcaac tgccgttgta gataaaggca agctaaaagg aataattacc gatggtgacc
    17161 ttaggaggat gttgcacaga agtgacgata tgttggccac aaaggctaaa gacattatga
    17221 cttcaaagcc caaatccgtt agccccgaca ctatggctgc cgatgctttt attctgatga
    17281 agtcaaataa tattacgcag ttggttgtga ctgataaagg taagtatctt ggttttattc
    17341 accttcacga tttgcttaaa gaaggcatat attaatcttc aaataaagaa atgtttttca
    17401 gaaggaagcc cgaccccaac tcagaaatga attttctgga tcacctcgaa gccttgcgct
    17461 ggcatttggt gcgtgctaca ttggcaatat ttgtgttagc cgttgtagca tttatatata
    17521 aggacttatt gttcgataaa attattttcg gacccaagaa ccctcatttt attacctaca
    17581 gattgctgtg tatgctttcg gagaaatggt caattgactt atgcattact gatattcctt
    17641 ttcacttgat aagtactacc atggcaggac agtttaccac acacatggta gctgcgggaa
    17701 ttgcaggact tattttagga tttccatatt tgctttggga gatatggcgt tttgtgaaac
    17761 ctgcattgtc tgccaaagag aagaaatata caagaggtat agttttttct gcttcgtttt
    17821 tgtttttctg cggagtggct tttggttatt ttattctggc accattatca attaactttt
    17881 tgggaagtta taaagtgagt gaagaggtgc agaatgctat cacgctcgat tcatatatca
    17941 gcattgtaag catcatgaca ttggcttgcg gagcggtttt tgaactgcct atactcgtgt
    18001 ttttccttac aaaaatgggt atcataagcc cacagtttat gagacgctac cgtaaacatg
    18061 cttttattgt taaccttgtt gttgctgcat taattacgcc atcaccagat gtaaccagcc
    18121 agatgttggt ggctattcct ttgtttctgt tatacgagtt cagcatattt gtttctgttg
    18181 ttgtggcaag gcaggagagt aaaaacgaat aaaaaataat cacatgagta aaagtgtaga
    18241 tgagttcaat gactatcgtg ccaaaatgaa taagcgcatt cttgagtcag ataatctggt
    18301 tttaaaacgc ctctataatc tcgatgccaa cacctatatg gatggtgcat tgccggttca
    18361 gactaaagaa atgcttgggc ttgttgccag catggtgctg cgctgtgatg attgtattaa
    18421 ataccatcta atgcaatgca aacaaaatgg tattactaca gagcaggttt atgaagtgtt
    18481 tgcagtagcc aatatagtgg gtggaaccat tgtgattcca cataccagaa gagcagcaga
    18541 attttgggat gagatttaaa tttctttttt tagcctgaac gaaagatagg ctgcttagcg
    18601 gctaaatagt taatccttaa caacccacct ctggcgcaag tcttcggact tgtgcccacc
    18661 tatcattcaa gcagcagtaa gtccagtaaa cctttggtgc cgccataatc acatgcactg
    18721 ctgtaaacat agtcttctgc tttataaaca aatcccgccc gcacaggatt ttcgtgaata
    18781 taatttaatc tttgttcctg catagttgca tccgttaatt caattgggtg attatcctgt
    18841 tgccaaaact gccagttagt attgttggaa ttattttctc ctgcctgctt aaagagttta
    18901 aacatccatt cgcgcctgct ttcttgcgga ctttccatta tggcgccaat atttttataa
    18961 gcagtaaact ttttaaggtc gcgcatgatg tcaggtaata ccctcaagct gtttttagaa
    19021 atgatcatat gtacatgatt ggtcatgata acccatgcat gaaccactaa gcctttttct
    19081 ttttgacagt gtctaatgct gtccaacaaa atatctttat aagtattgcg ggtaaaaaca
    19141 tcaatccagt ttacgaccga aaaagtaaca aagtaaatgg catcaggctc ttgaaatttg
    19201 tacttggtac tcatgtatgc aaaggtgcaa cttctattga ttgcaaatat tatttgagca
    19261 cggtaaaagc cagtttagcc tgcttgctgc tttgttacac taaaattact gcactgcatt
    19321 ctttattgat agatcagcac aagtccgaag acttgcgcca gagggtgttt tatttaaaat
    19381 tcgttgactc gttgtagtac gcaaagtaaa ctcaggtctg ctgtcttttt ctacactaac
    19441 cattattctt taataacctt tacacttctc tcaacacttc cccattttaa cttgcacagg
    19501 taaatccctt ttgccaatgc ttctatattc actctcttat actggctcca tggtgccaca
    19561 tattccctca tcaccaaatt tcccatcaca tcataaactt ccaattctcc ggcagtactc
    19621 tgcacattaa attgcaaggt aaaattccca ttgctcggat tagtcatggc aacaactccc
    19681 cctttagggg gctggggggt aggcagccct accgttggcg cacacaagca ccctaatgtg
    19741 ctgtcgcatc ccaaaaaata attaggatga tttggcagcg tattaaaata aacagattgc
    19801 agttgaacac tgtgttggtt cacattgcat cccaaaccaa ttacatcagg gttatctatc
    19861 gtgtgcatat gtatagtgcc attgcctgtg gtaatgtaaa tttttccatc cggtgcaagg
    19921 gcagataaac cgaacaatgt ggcaaaacca ctgggaaacc ccgggaaaac cgatacaaac
    19981 gaatcataag ttgcaactgt taattgcgat gcagcaatat ttgctgctgt taaatcaaat
    20041 tgatacacat gttgtgtatt agaaacatat aaggcattag accccggaga aattgccata
    20101 ccgacattat agccgttctc aaatggcatg gtaacatgca ctgcattact tagtgttgcg
    20161 gtgcatctgt caaaatcaaa aatatccaag cctccgttca cataataata agcatatttt
    20221 ttcccgtcct gtgaaaacca tgcttgtcct gcatcccatt gtcttgtaac accaatattt
    20281 tgcgtgtagg ggcctaaaat aacagtaggt gttatcaata gctttataaa ttgattagag
    20341 tttacccggt gaaccatcac ccaccagtct ctgccattag catgtctggt agcagctatc
    20401 ttccctgtat tgagtgtgtc aattattaca ggaacatttt ttgttattac atttcctaat
    20461 ccgccattgc cattcatatc aatggttgtc aagtaaagtt ggtgcatcct gcatcctgtt
    20521 gtcatcatat aattatcgca ggaaccatga aacatgtaat aaatggaatt actacttggt
    20581 ttttgaataa tcaatgcagc ctgcgctatt gcaaaaccat ctgatgcaaa gtttgcatat
    20641 gctccgggat tgattccgct accgttttgc attgtatcat tagtcgcatc tgctaaataa
    20701 tatccattgg tgtaaaacat catgttacct gttgcatctg aaatatttcc atgagtatgc
    20761 ctgaaatcca tctccaaact atcataagac aaaacgggag taccattgta gaaatcaatt
    20821 ttcgtttgcc caaagggttg gccaccccaa cttgaatacc ccatcagcca gttattattt
    20881 attccttgtt gggcaacaga aattgcatac attaatagga aaagaattat aagtagtggt
    20941 ctttttttgt ccataatcaa aggagttaaa taaagggagg aaactgtttt caatttcccc
    21001 cctgaataat tttaacgtat aattattaat ttaccatttg ctattaactc accattgctt
    21061 ctcaactcat aaaaataaac tgatggtttt aaatcagccg tgctaaaaga gtattttgaa
    21121 tcgcccgctc gtaaaggctt atttaaaatt tgctggtttg tagttgttcg tataataaat
    21181 tcagccttgc tgtcttttgg taagctgtaa tccaaagtag ccatttcatt tgccggattc
    21241 ggataaacag ttgcaacagt atctcccttt gctttttctg gttttctcaa agcaatacca
    21301 acaaggttgc aaattttaaa atcatcgtac tgcttatttt tatcaaccaa agcatatagc
    21361 gaccgtgcca taaaaacagc attgccgcct tgcatggggc attgtatggc tatgctgtac
    21421 aatgtttcaa tatcatcctg cgtaaattca aatacatcgc tgccaatggt gcgcaggtaa
    21481 atttcattta ctatctgctc attttcctcc ggtgtattgg tggctgccag tatgttatta
    21541 cttattccaa tggctgttgc tgtagtaatt ctgtcggcag tttcatcggc tatcacatct
    21601 tcagccaagg tgttggtggc agttactgtt tcctgcaaat ccataatatc atctgatagt
    21661 attcgcaccg ttgccgtgtc atcatttaca aatgccgtat ccaataaaac catttgctgc
    21721 tctataagcg ttaactgctg cgctgcatcc gccctgtatt gctgcagcaa agcttccgaa
    21781 aaattatcgg gcacaaacaa ttcattctta tcagcttcca caggcaccaa ttctgccaaa
    21841 gttgtgccct gcatggcaat ataaaaattg ttaagaacga catcattttg cagcaccgga
    21901 ttttcttcaa tccttttaaa caaatccttt ttaaacatcc accgtgtttc atccgtgtaa
    21961 gggtcattgt caatttggtc ggttgctacc aaataatcaa aatcggttgc cacattccta
    22021 aacgaccttg atgcacatgc aaattggtca gtggcgcact cataatttgt tccagcagca
    22081 ggttgaaacc aatcatcagg gtcttgaaaa ttattatgag gtaataaggt atttgtaccg
    22141 ggtggcccaa catcattcca agtaaaaact ggattataaa acggattctc attcaatgct
    22201 aacctgaaac ccgcaggtgg ggttggcaca ttccatacat taccttgtaa atcctggtca
    22261 gcagtagttg ccgatacact atacctgaat gcattcccgt ggtcgtaaaa atcattccct
    22321 ttcagttctg tatgggttcc gccaacagcc gccaccacaa acagtccggt gttggaataa
    22381 agtgctgaaa aatctgcttc cagctctttc cagtttattt cttctgatag taaaaccaac
    22441 tggtggttca tatctataaa ctctttgagc agtggctgaa ataaattttt ctgatcttgt
    22501 atgggtgatt tgcctatcat ttttctgcaa ccttttttta caaaaataac ccactttgtt
    22561 gcagttttta tcacctcttt ttctaaagtt tttaactttt tatagagaca cggaaggggt
    22621 tgttaaggat taactaatta acttctatta ttttgtacat gccacaccag taggtcagca
    22681 aaatgcaaaa aattctttat aaatacacta ttatcaagga tttacaaaaa gttgtctttg
    22741 aaggtggaca agtcagggga tttaaatttc tttttttagc ctgaacgaaa gataaaaaat
    22801 aggctgctta gcggctaaat attttttttc ataggtggtt tgaatgtcca tgtcaacggg
    22861 catcagtaat tctttatata aatctgttgt ttgatgtaat attatatgac cggcatcatt
    22921 tacagtttgc aaggtaaatt catacaaatc tttattatct gtttttaaat gtacaattcc
    22981 atcaggctgc aaaaaagtgc tgtatctttt tagaaaaagg ggagaggtta atcttttgtt
    23041 ttctttcgac tcctttggct gtgggtcggg aaaggttatc caaacttcac ttacctcatt
    23101 agctgcaaaa aacttttcaa ttgtttccat tcttattcgc agaaaagcag cattcattat
    23161 cttttcttcg ttgatggttt ttgcgccacg ccatattctt gcacctttta tgtcaatacc
    23221 aataaagttt ttttgccgat agcgttgcgc caaagcaaca gtgtattcac ctttgccaca
    23281 cccaatttct aagactatgg gttgttgttt ttgaaaaatt gtttcgttcc acttgccttt
    23341 taactcaaaa tccggtgaat ggtagtttat ttccggttga attactctct caaaagtctg
    23401 catttcagca aaacgtttta atttgtcttt ccccataatc ttaatgcccg actaacggta
    23461 atggtattta cgaagttgtt aaattatttt acctgaccgg atggcaatgc aattaaattt
    23521 gaaaataaac gatatgcacc ggacacacct gccggcaatt gcctgaaaaa agcaagccct
    23581 gtatagacaa aatgtccttt gcctgattgt gcaattatca gacttccatt caatgggctt
    23641 tctcctttgt cattgcaact taaaggtgtc tgatactttg ggtcaatctc ttctgcaaaa
    23701 tatattccac gctcctgaac ccagccttta aaatcgtctg catttatttt attgggtttg
    23761 ttcaatacaa tggaatttgc ttctgaaaat cgcatttctg catattcatc cgttaccctt
    23821 tttcgtgaaa ttttaaatgg gtagggacca atgttgttat caagtttacc taaattattg
    23881 ttagtgttgt attgaacaat atatgtacct cctgaggaaa caaagtccat gagtttgttt
    23941 ttataaccca taagccagtc gttggtgtta taagctctta cacctgcaat aatcacactg
    24001 taatttgaca gattgtcgtt actaagattt tcttctttta aaacagaaat attatatcc
//
LOCUS       DAOOOI010000030        23758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_397_length_23758_cov_58.888495, whole genome shotgun sequence.
ACCESSION   DAOOOI010000030 DAOOOI010000000
VERSION     DAOOOI010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 23758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23758
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_397_length_23758_cov_58.888495"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PKX59_05580"
     CDS             <1..491
                     /locus_tag="PKX59_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOZ90372.1"
                     /translation="AAKVSGQCLGGGMELALMCNFIFADKTAKFGQPEIILGVFPPPA
                     SILLAEKIGLARAEDLLITGKTITADEAKSIGLLNDVFDDKETLNAGLNNWIETNILP
                     KSASSLRFAVKAVRAKMNHILSNFLPQLESMYVNQLMQTEDANEGINSFLEKRKPVWK
                     NS"
     gene            501..2504
                     /locus_tag="PKX59_05585"
     CDS             501..2504
                     /locus_tag="PKX59_05585"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HOZ90373.1"
                     /translation="MKNIRTVGVVGAGTMGAALAQKFAQEGFEVILADRAKNFVEKGL
                     NNIRITLEDGVTKNVFSKQQVDTFLSHLKGTDSLDDLKVCDLVIEAIFEDLQAKKDLF
                     SSLSSIVSSDGIVATNTSSFSVTELAASMVNPERFIGLHFFYHAAKNRLVEIIPGEKT
                     TSETLQAARLFSVLAGKDAIDCTDTYGFAVNRFFVPWLNESVKLLQEGIATKSEIDAV
                     CMKVFGIGMGPFALMNATGVPIAYHAEKTLEVFGKLYEVASLLKSQAEAAKPWEIEPA
                     TADTIESKKVKLISDRMLGVVFYVCSQILEEKVSSAIHLNRGAKIGLRWKKGPVDLMK
                     SAGETEVIRLVTLMSELYQMRIPSSVGKKFWQMESVTIKKNKSVAVITMDEPENMNAL
                     SEETMQGLSEKYAEADNDPTIETIFITGSGKAFVAGADIKFFVRNIKTGKISDIETFT
                     AFGQDVFNRIDRSNKKVVAIVNGMALGGGLELALCADRILAFPKAQFAFPETGIGIYP
                     GLGGTQRSAAKIGQGLSKYLIHTGKMLNAKDAEEIGLVDKIITVEDFMEMVEGKKQVP
                     ASSEKALPGKWSMIKDFFEDNTLSEIVEKKYSGNALPAEEVEKIVKTIGHKAPVALRL
                     ADKLIEEKKGCASELEHLSEIFSTSDALLGLTSIGKKIQYEGK"
     gene            2501..3112
                     /locus_tag="PKX59_05590"
     CDS             2501..3112
                     /locus_tag="PKX59_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOZ90374.1"
                     /translation="MKSSVSAIILAAGQSKRMNRPKPFLAFNEMENFIEKIGSTYLAA
                     GIEKAIVIVNPDIEREVSVLLKNKFSDSKLEVVVNSYPEKGRFYSIQLGLEKTDTDSC
                     FIQNTDNPFVTKDLLNNMCRMIKQGAYVVPVFKNEKGHPVIISAEVMNHCRKLNGNDF
                     NLRDELQMFEEIRLVWDEKNILANINTEDEYQKYFPSCEAFTK"
     gene            3091..3927
                     /locus_tag="PKX59_05595"
     CDS             3091..3927
                     /locus_tag="PKX59_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOZ90375.1"
                     /translation="MRSIYEIIAGLEQCGEEAALCIITETRGSTPLKAGAKMIVHRNK
                     NISGTIGGGNLEKQVIEDAVSVMESGKAATFEHQLVRDHQMCCGGSVFVFIEPVKKRN
                     KLIVFGAGHVGKEVAKLACSLNFKISLVDERKEMLTGLALDDAKIYNMHHLQFLSDVV
                     LDENTFVVICTHLHHYDREILARCIKQPYAYLGMIGSMRKVVITRKKFISEGLASEEE
                     LSKVDMPMGFDIGGNSPAEIAISIVAKLVAVMNGHTMQNNNEEKSNYTYAGQDSDNNR
                     CW"
     gene            3890..4627
                     /gene="fabG"
                     /locus_tag="PKX59_05600"
     CDS             3890..4627
                     /gene="fabG"
                     /locus_tag="PKX59_05600"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOZ90376.1"
                     /translation="MPDRIAIITGAGKGIGKAIAERLSADGFYVALVDVDQAAMEEAV
                     AIIGADKATGYLVDITNENQVKALFENIENAKNKIDLLVNNAGIIRDNLIWNLSANDF
                     DAVINVNLKGTWLMCREAAIRMKQQKYGCIVNIASRAWLGNAGQTNYSASKAGIIGLT
                     RSLALEMGRHNVSVNAIAPGLIDTPLTQKLDKDVLDKLIQAQPSRSMGKPEDIANVVS
                     FLASEKTSFMTGQTIYVDGGKSIGAGL"
     gene            4655..7450
                     /locus_tag="PKX59_05605"
     CDS             4655..7450
                     /locus_tag="PKX59_05605"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOZ90377.1"
                     /translation="MRSIKIKVNGRQHEVLIDEHETLAHVLREKIELTGTKIGCEQGS
                     CGACTVLLNDEPVLSCITPAMRCDELSVTTIEGVSENGELHLLQQKFVEKGAIQCGFC
                     TPGMVMTSLDFVRKNPHATVDEIKEALSGNLCRCTGYKKIIEAVAETIRPQQHPAEAN
                     GSKPHNGRIVGTGMPYIEAAKKVQGKAEYADDIRLKNVLHCSFLRSPYPHAKIEQIDT
                     SAAAKLEGVKYILTGVELPEKFGVLPISQDETALAIDKVRYIGEVVAAVAAETEAIAK
                     AACNLIQVKYKPLKPFFEIDESLNDIGTEEKIHEHVKFNNNVHKKAELRFGNQEEGFQ
                     SAAFKSKMNFEFEGITHGFTEPHAATAYWDENGLTIITATQVPHYLHRALSKVLKLPL
                     SRVRVIKPYVGGGFGGKSDPFPHEIIVSYLAIKTGRPVKVRFSREEVFISNHGRHPTK
                     MTMEIASDETGNFRALDADIAIDGGAYGSFGVVTTYYNGVLLQAPYKVENFGFKTFRV
                     YTNKPQCGAMRGHGAVNSRFAIETLIDDLAHQLKMDPCELRLKNFMPSNTLTAGQYRI
                     TSNGSRESLLKVMEQSQWKERYGKLPFGHGLGVGCGFFISGSALPIIRNELPQSVVHL
                     KVDFDGRVLVTSGASDIGQGSDTMLAMIVAEVLGISMDKIFVLAADTLLTPVDLGSYS
                     SRVTFMAGNAAKMAAENLKKEIINSVVARKEISADELNLADDCVFSNDRKIELTWNEA
                     IDFTIAHRGAINVSGKYISPKLGGDFKGSGAGLSPSYSFGAVVTEVKVDPETGAVKVL
                     NIWGAHDCGKAINPLAVEGQLEGSWHMGLGQAMSEQVKYFNGLLINSNFLDYKIPTSL
                     DTPPIHTNIIETADPEGPFGAKECGEGAIHPIIPAVANAVFDAVGARITKLPISPEDV
                     LQKIKNLSLPNAAQVNV"
     gene            7459..8391
                     /locus_tag="PKX59_05610"
     CDS             7459..8391
                     /locus_tag="PKX59_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOZ90378.1"
                     /translation="MSDNKKYLKPVSVHEAITMSKVCGDDFRYIAGATDVMVNRRQGN
                     EESFCLIDLCGIDELKQVTVKENYLSIGSLVRLDDLKKHAAIVKEFPVLIEAAETVGS
                     PLIRKSATLGGNVLCENRCIYYNQSEWWRDAVGYCLKCEGNICIATGSKKLCYSEFVS
                     DTAPALISMDAEIEIADHEGERTLKLEDIYTGDGVCPRNLKKSAVIKSILLPLQREFH
                     SSFHKLRQRQSLDFTSLTVAVSIDKNGKLKSVLGGVDPKPVVVEGNSDSNLEEIIKLA
                     LKGSRAIDNEMFSRNYRRSMISVFLKRSFEKLIG"
     gene            8399..8986
                     /locus_tag="PKX59_05615"
     CDS             8399..8986
                     /locus_tag="PKX59_05615"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOZ90379.1"
                     /translation="MISDKSIEVFVLAGGKSSRMGEEKGLMLLHNKPMIQYVLEQLSI
                     LKLPVQIIANADDYTKFGYDTLKDIIAEKGPMGGLLTAFHYAKSDCVLLISCDMPFIT
                     SEIVSKLIFEVKDDEITAAYVQNEPNPLFAVYKKSLKNEVEKRIAAGNLQMKHLIKSF
                     RHKRVEMDVYLNRNPNLFINLNSKQDVNNITDVWN"
     gene            8977..9219
                     /locus_tag="PKX59_05620"
     CDS             8977..9219
                     /locus_tag="PKX59_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOZ90380.1"
                     /translation="MELKGIKILAFGMIAEKINASEFYLKEVSNTNELLSSLNSLYPQ
                     LKEMKFTISVNRRIVNNNEPLNADSEVALLPPFSGG"
     gene            9230..10327
                     /locus_tag="PKX59_05625"
     CDS             9230..10327
                     /locus_tag="PKX59_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOZ90381.1"
                     /translation="MSRFERQINLSGFGPKAQQQLQKAKVLVIGAGGLGCPVLLYLDA
                     AGVGTLAICDGDVVAASNLNRQVIFSESDITKSKAEVAGIYLKQKYSDINIEIINKFL
                     TVENTLQIFNAYDLIIDGSDNFQTRYMVNDACVLLNKPLVFGAVYQNEGQVAVFNVSD
                     DNAVSSNYRDVFPNPPSENEILNCNETGVLGVLPGIIGVMMASEAIKLITGFGKALIN
                     KMLYYNLLTNTTYETEIAPNAVAKSIIPKTPEDFYKTDYTLLCGNLPSIEWSEALLLK
                     NNTPGKVILLDVRETHEQPKLDGIETMNIPLQSLSENIEPLNTAENILVFCQSGIRSR
                     KAVAVLRSKFLQKNIYSVAGGISKLNSTINA"
     gene            10333..10773
                     /locus_tag="PKX59_05630"
     CDS             10333..10773
                     /locus_tag="PKX59_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HOZ90382.1"
                     /translation="MERKPKQIFIQGAISSSKIADSIQKHSSKTDIGAHSIFLGQVRT
                     DEKPEGIIQAIEYTTYEEMALQKAFEIREDIFAKYPLTCLHIYHSLGVVKTGEICLFV
                     FTSSKNRKAATEACNEMVERIKNELPVWGKEIIDEKKSLWKENS"
     gene            10780..11691
                     /gene="moaCB"
                     /locus_tag="PKX59_05635"
     CDS             10780..11691
                     /gene="moaCB"
                     /locus_tag="PKX59_05635"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009392204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HOZ90383.1"
                     /translation="MINITHKNSTHRTAIAQAIVKVSKEETITAVKENKVPKGNVFEM
                     SKTAGLFAAKRTSDMIPDCHPLPIEYAHITHEINGLEILIRVEIQTIYKTGVEVEAMH
                     AASVVALTIYDMLKPIDKNISIEQIKLIEKKGGKSDFIKKEISGIKAAVIVCSDSISK
                     GTKEDKAGKAIIEKLKSCKVDIADYIIIPDEKNIIQEKVRAYCSQQIQLVILTGGTGL
                     SPRDITPEALDSVIEKRIPGIEEAIRTYGQERTPYSMLSRSIAGLVGNTLVLALPGST
                     RGAAESMEAVFPHILHIFRVMKDARHD"
     gene            complement(11722..13362)
                     /locus_tag="PKX59_05640"
     CDS             complement(11722..13362)
                     /locus_tag="PKX59_05640"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOZ90384.1"
                     /translation="MAEKVNLEFNINEDAYKSHLIRLAERLNKIYEGGGKKNAAKQKE
                     KNKMLARERIAYLLDTDKPQFEVGAFAADGMYAEQGGCPSAGVVIVIGYISGKQCIVV
                     ANDATVKAGAWFPMTAKKNLRAQEISMENKLPIVYLVDSAGVFLPMQDEIFPDKEHFG
                     RIFRNNAVMSSMGILQIAAIMGSCVAGGAYLPIMSDEALIVDKTGSVFLAGSYLVKSA
                     IGEDIDNETLGGATTHCEISGVTDNKFPDDASCLDYIKRLFSKIGTFDKAGFERAESK
                     EPAANPKEIYGIIPAENSKQYDMHEVINRLVDNSEFDEYKEKYGQSILCGTARIDGWA
                     VGIVANQRKVVKSKKGEMQFGGVIYSDSADKAARFIMNCNQRRIPLVFLQDVTGFMVG
                     SRSEHGGIIKDGAKLVNAMANSVVPKFTIIIGNSYGAGNYAMCGKAYDPRLIAAWPTG
                     QIAVMGGSQAAKTLLQIQVAALKAQGKEISEEDEKAQLKTITDRYNSQTSPYYAASRL
                     WVDAIIDPLETRNMISMGIEMANHNPITKPYNVGVIQT"
     gene            complement(13432..13608)
                     /locus_tag="PKX59_05645"
     CDS             complement(13432..13608)
                     /locus_tag="PKX59_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HOZ90385.1"
                     /translation="MAFTDKFEKDEYYLTPEGYIVFTEKYHLKRGYCCQSGCRHCPWN
                     YNTKKADKNNKQKS"
     gene            13736..14641
                     /locus_tag="PKX59_05650"
     CDS             13736..14641
                     /locus_tag="PKX59_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOZ90386.1"
                     /translation="MNSPIIDEVNLNDFQLAHKGVSLHVLRLDLDDVITGGNKSFKLK
                     YNLQAAKEKGRSIIVTFGGAFSNHIAATARTCKLQNLKAVGIIRGEIVTPMNNTLSRA
                     VNDGMTLIPVSRQWYRQIHDKEFYKSNLVKCLLIANNINPDACYVVPEGGNNAEGFKG
                     CLEIMNNINADYDTVVCAVGTGTTLAGIAASLPQNKNVHGIAVVLAADTLTKNIQLHL
                     AESNSNAKYIVHQQYSFGGFGKSNAELKTFCELFTQKTNILIEPVYTGKLFYAILDML
                     AKDKFNPGTKILAIHTGGCQYLIDK"
     gene            complement(14694..16274)
                     /locus_tag="PKX59_05655"
     CDS             complement(14694..16274)
                     /locus_tag="PKX59_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90387.1"
                     /translation="MRKHLQKLAVLGMFSMFAYTAQAQRFLTEVFPSVNVTPNVVYGQ
                     NYEVLTGTPVLKDLKMDIYEPAGAADPMTERPLIIYLHTGSYIPIIVNGNATGSRNDS
                     AAVEICKQFARRGYVVANMDYRLGWDPTNTNPNNGLDIRRGTLLNAVYRSIQDAKSCV
                     RYFRKDAATNNNVYKIDVNNFYLVGQGTGGYIALNYAVLNSPSEITLPKFISSTDAPT
                     YGIYAGQPYVNQTALGDFDGIGGVAPYNNENNPGYSNAVQFVVNLGGAMGDSSWIAAG
                     DAPMIGIHCVDDPFAPYGVGIVYVPTTPPQAVVDVIGSGEIIKWANQLGNNACFANAG
                     FTDPYTLRANSINNGNEGLFPLQQGINPVNPQLGHAGPWEWYDLQSTRNLAMAMGYQS
                     TRGDTCYGNSMLTNPTMSKTYALAYIDTIMNYVNPRIVFCRNLPTSVKEVSTNSSDVT
                     IYNSPSKSYFTVVSTSKEPMRSIEVYDIKGSLLASEKNPNAFNQRIEKASLSLNKGVV
                     LVKITYNSDVITKKITLD"
     gene            complement(16340..16984)
                     /locus_tag="PKX59_05660"
     CDS             complement(16340..16984)
                     /locus_tag="PKX59_05660"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOZ90388.1"
                     /translation="MNAKKEKELSPLDNITDFEVFISGRLKDLPGRNSHLKLASVNRP
                     VYEPEGNYRIGSVMLLVNNKNNIPHLVMIQRAEDGKVHSGQIAFPGGKAEPDESLTLT
                     ATRELEEEIGIADKSINIIGQLSRIYIPPSNYMVYPFIATQIQTSVFLANQNEVKEIF
                     EIPLHSLIKNKTIIQGLQHQTTRGLITAPAFRIGNLVIWGATAMILSEFIDLID"
     gene            complement(16947..18719)
                     /locus_tag="PKX59_05665"
     CDS             complement(16947..18719)
                     /locus_tag="PKX59_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ90389.1"
                     /translation="MKILVTYLKRHWKLVLLALLLAAVNQCFSLLDPLIAGKMLDRFA
                     MHVSNYSNDFPKFFQDIIWFLLASMGVALVSRIAKNFQDYFVNVIVQRTGAEMYSDGI
                     KHSLELPYQVFEDQRSGETLGKLQKVRADSEKFINAFISILFQSVVGIIFVMAYAINV
                     HWSIAPVFLLTVPVLAVVSNMLSKKIKVISKKILGETTALAGATTESLRNIELVKSLG
                     LANQEINRLNTVTDKILNLELKKVKFIRSLSFIQGTTVNFLRTLFVFLLYYLIFKDEI
                     SPGKMMTLMFYSFFIFGPLQELGNVIAVYREAQASLNNFSEIMEMPLEYKPQSPLQLN
                     AIETFEFSSVSFKHISAHGNAVNNISFKARRGETIAFVGPSGSGKSTLVKLLVGLYHP
                     AEGKILYNGIDANNINLNELREQIGFVTQDTQLFAGTIRENLLFVNPHAHDDQVLNAL
                     NMASCHSLLARAENGLNTVIGEGGVKVSGGEKQRLSIARALLRNPNLLVLDEATSALD
                     SITEEEITETIKSINKNKNLITILIAHRLSTIMHADHIFVLEKGHKIEEGRHETLLEE
                     KGLYYAMWRQQIGERKKGERVVTA"
     gene            19072..20151
                     /locus_tag="PKX59_05670"
     CDS             19072..20151
                     /locus_tag="PKX59_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HOZ90390.1"
                     /translation="MQKIVKLYEWTTSLPIITCLLYFSGLLDNNIVLQILAGILLLFC
                     VMSAVHHSEIVAHRVGEPYGTIILAVSITIIEVAIIISLMMTGGKEYASFARDTVYAA
                     VMLILNGIIGLSLFLGGRKYHTQTFSPHSVKIALVALVSIVTFTMILPTFTSSIIGPY
                     YTERQLLFEIIACLIIYIAFIAAQTIRYREYFITEANPDVEVTHNNISNSSFIISLIF
                     LMICLVIVVLLAKSLSNPIENLLTSYGLPRSLVGIVIAAVILLPEGIAAVSSARKNKL
                     QNSLNLALGSALASIGLTIPAVSLASYIFDLPLILGLELLPIILLAISIFTVMLSLIG
                     GRSNIVYGVVLLVNLFAYVFLTINP"
     gene            20543..21319
                     /locus_tag="PKX59_05675"
     CDS             20543..21319
                     /locus_tag="PKX59_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90391.1"
                     /translation="MFFSDAIGNKDTITLGYDLTATDSIDTAFGETNIISVPLDTNLD
                     VRITNAWFTYADAVYFHLKKQIVFYDCSLHNLNIQPINIVTQHWPVTMTWNNSIFNDS
                     CNNGSVFTGINPAGWWDTGCPSGLLQQVFLGDDSITFNSNFDLGWFGYINSNGDSIPV
                     FWQAFGDSTILTTGINELPQQCISVKVFPNPGSTYITVQANNSFGQLSLISILNSYGQ
                     IVLKTQQISRIDISELSDGIYFIKTENTKGEFTTSKFLKL"
     gene            complement(21622..22464)
                     /locus_tag="PKX59_05680"
     CDS             complement(21622..22464)
                     /locus_tag="PKX59_05680"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HOZ90392.1"
                     /translation="MKKQFIFLTAILLFLLFNACRKNDNTDEVSPNVPDSTSITNSLG
                     TMPSVFEQRVLIEELTGEWCPGCVNGAHILQSIKDSAINGGKVDVVSIHDVDWLEIVK
                     YDTVRFHYWQAMFGFPCATVGRVVQPSTGTYYVDYTKWQSNVTSRLAETHDLGIALIS
                     KLENDSGRVEVHIGHNSHINDNLRLTIFVTEDNVLAQNQLGSVGNFFHQQVVRLMPTN
                     ALGDDITFTSTETVMSYSFPVVGYKLSDLKIVAMVSSWNYNPIQSKIWNSRTVKFGQI
                     AKWK"
     gene            complement(22864..>23758)
                     /locus_tag="PKX59_05685"
     CDS             complement(22864..>23758)
                     /locus_tag="PKX59_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOZ90393.1"
                     /translation="TNMVFDKFPELIKDIKGLSEETTTGVHRLYERMQKGTLPIPAIN
                     INDSVTKSKFDNKYGCRESLQDAIRRATDLMLAGKVAVVAGYGDVGKGSAESLSSAGV
                     RVIVTEIDPICALQAAMEGYEVKTMDNAIPEADIIVTATGNHNIIVDKHFKAMKHNAV
                     VCNIGHFDNEIDMAWLNKNYGNSKDTIKPQVDKYTLDGKDIIVLAEGRLVNLGCATGH
                     PSFVMSNSFTNQTLAQLELWNHTDKYENKVYTLPKHLDEKVARLHLSKIGVELETLTQ
                     SQADYIGVPVAGPYKPDYYRY"
BASE COUNT         7590 a         4434 c         4595 g         7139 t
ORIGIN      
        1 ttgcagcaaa agtatccggg caatgtttgg gtggaggaat ggaacttgcg ttgatgtgca
       61 attttatttt tgctgataaa actgcaaagt tcggtcagcc cgaaattata cttggtgttt
      121 tcccgccacc ggcttctatt ctgctcgctg aaaaaattgg cttggctcgg gccgaagact
      181 tattgattac aggcaaaacc attactgccg atgaagcaaa atcaatcggt ttactgaatg
      241 atgtatttga tgataaagaa acattgaatg cagggttaaa caactggatt gaaacaaaca
      301 ttttacccaa aagcgcatct tctttacgtt ttgcggtgaa ggcggtaaga gcaaagatga
      361 atcatatact gagcaatttt cttccgcagt tggaatccat gtatgtaaac caactgatgc
      421 aaacggaaga tgccaatgag ggcattaact catttttaga aaaaagaaaa ccggtttgga
      481 agaattcata ataaaaagca atgaaaaata tcagaacagt aggagtagtt ggcgcaggaa
      541 caatgggcgc agcattggct caaaaatttg cacaggaagg ctttgaggtt attcttgccg
      601 accgtgcaaa aaatttcgtt gagaaaggat tgaacaatat ccgcatcaca ttggaagatg
      661 gagtaacaaa aaatgttttt tcaaaacaac aggtagatac atttctatca catttaaagg
      721 gaaccgattc actcgatgac ctgaaggttt gcgacctggt gatagaagca atttttgaag
      781 acctgcaggc aaagaaagat ttgttcagct cgctgagtag tattgtttca agcgatggta
      841 ttgttgccac aaacacttct tcattttctg taaccgaact ggcagcaagt atggttaacc
      901 cagaacgatt tatcggactt cacttttttt atcatgctgc caaaaatcgt ctggtagaaa
      961 ttattcccgg tgaaaaaact acttccgaaa ctttacaggc agcccggtta ttttctgtgc
     1021 tggcaggcaa agatgctatt gattgcaccg atacatacgg atttgctgtg aaccgttttt
     1081 tcgttccatg gcttaatgag tcggttaaac tgttacagga aggcatagca acaaaatcag
     1141 aaattgatgc agtatgcatg aaagtatttg gtattggtat gggtcccttt gcattgatga
     1201 atgcaacagg agttcctatt gcttatcacg ctgaaaaaac tttggaggtt tttggtaaac
     1261 tttatgaagt agcctccctg ttaaagtcgc aggcagaagc tgccaaacct tgggaaatag
     1321 aacctgcaac ggctgataca attgaatcaa aaaaagtaaa attaatcagc gaccgtatgt
     1381 tgggtgttgt gttctatgtg tgttcgcaga ttcttgaaga aaaagtttct tcagctattc
     1441 accttaaccg tggtgcaaaa attggcttgc gttggaaaaa aggaccggtt gatttaatga
     1501 agtcggctgg agaaacagaa gtaatccgcc ttgtaacatt gatgtctgaa ttataccaga
     1561 tgcggattcc atcatctgtc ggaaaaaaat tctggcaaat ggaatcggtt acgattaaga
     1621 agaacaagtc agtggctgtg attaccatgg atgagccgga aaacatgaat gcgctgagcg
     1681 aagaaaccat gcagggcctt tcagaaaaat atgctgaagc tgataatgac ccaaccattg
     1741 aaacaatttt catcaccggt tcagggaaag catttgttgc cggtgctgat attaaatttt
     1801 ttgttagaaa tatcaagaca ggaaaaatca gcgacattga aacattcact gcctttggtc
     1861 aggatgtatt caaccgtatt gaccgttcaa ataaaaaagt agtggcaatt gttaacggca
     1921 tggcgcttgg tggcggtctt gaactggcat tgtgtgccga caggattctt gcatttccaa
     1981 aagcacagtt tgcatttcct gaaactggca ttggcattta tccgggattg ggtggtacgc
     2041 aacgcagtgc tgctaaaatt gggcaaggac tgagtaaata tttaattcat acgggcaaaa
     2101 tgctcaatgc aaaagatgcg gaagaaattg gtttggttga taaaattatt acagttgagg
     2161 atttcatgga aatggttgaa ggcaaaaagc aggttcctgc ttcaagcgaa aaggcattac
     2221 cgggaaaatg gagcatgata aaagactttt tcgaagacaa tacactttcg gagattgttg
     2281 agaaaaaata ttcaggcaat gcacttccgg cagaagaagt ggaaaaaatt gtaaagacca
     2341 tcggtcataa agcccctgtt gctttgcgcc ttgctgataa attaatagaa gagaaaaagg
     2401 gatgtgcttc tgaactggaa catctgtccg aaattttttc aaccagcgat gctttgctgg
     2461 gattgacttc tatcggtaaa aaaattcaat acgaaggaaa atgaaaagct ccgtttcagc
     2521 aataatactg gctgcaggtc aatcgaagcg gatgaacaga ccaaaacctt ttcttgcttt
     2581 taatgaaatg gaaaatttca ttgaaaaaat tggaagcaca taccttgcag caggtattga
     2641 aaaagcaatt gtaattgtaa accctgatat tgaaagagaa gtcagtgtat tattgaaaaa
     2701 taaattttca gacagcaagc ttgaagttgt tgtaaattca tatccggaga aaggaagatt
     2761 ttattccata caactcggat tggaaaaaac ggatacggat tcttgtttta tacaaaacac
     2821 tgataatcct tttgtaacca aagatttgct gaataatatg tgccgaatga ttaagcaggg
     2881 tgcttatgta gttcccgtgt ttaagaatga aaaaggtcat cctgttatca ttagtgcgga
     2941 ggtaatgaac cattgcagga aattgaacgg aaatgatttt aacctgcgtg atgaactgca
     3001 aatgtttgag gaaataaggt tagtttggga cgaaaaaaat attttagcta atattaatac
     3061 ggaagacgag tatcaaaaat attttccttc atgcgaagca tttacgaaat aattgccggt
     3121 cttgaacagt gtggcgaaga agcggctctc tgtatcatca cagaaacccg tggatctact
     3181 ccgcttaaag ctggcgccaa aatgattgtg caccgaaata aaaatatttc cggcaccatc
     3241 ggtggcggca acctcgaaaa gcaggtgata gaagatgctg tgtccgtaat ggaaagtggc
     3301 aaggctgcta cttttgaaca tcaattagtc cgtgaccatc agatgtgttg tggcggttcg
     3361 gtgtttgttt tcattgagcc ggttaagaag agaaacaaac tgattgtatt cggtgctggt
     3421 catgtaggta aagaagttgc gaaacttgcc tgctcactga attttaaaat cagccttgtt
     3481 gatgaaagaa aagaaatgct caccggactg gcattggatg atgcaaaaat ttacaatatg
     3541 catcatctgc agtttttgtc tgatgtagtt ttggatgaaa atacattcgt agtcatctgt
     3601 actcacttgc atcattacga cagggaaatt ctggctcgct gcatcaaaca accttatgct
     3661 tacttaggca tgattggcag catgagaaaa gtagttatca caagaaagaa atttataagc
     3721 gaaggattgg caagtgaaga agaactttca aaagtagata tgccaatggg ttttgacatt
     3781 ggcggaaatt cgcctgctga aattgccatt agcattgttg caaaattggt tgccgtaatg
     3841 aacgggcaca ctatgcaaaa taacaacgaa gaaaaatcaa attatacgta tgccggacag
     3901 gatagcgata ataacaggtg ctggtaaggg aattggcaaa gccattgccg aacggttgtc
     3961 agcagacggc ttttatgtgg cattggttga tgttgaccag gcagccatgg aagaagcggt
     4021 tgcaatcatt ggcgctgata aagctaccgg ttaccttgtt gatatcacca atgaaaacca
     4081 ggtaaaagca ttatttgaaa atattgaaaa cgcaaaaaat aaaattgatt tgttggtgaa
     4141 caatgccgga attatccgtg ataatttaat ctggaattta tcggcaaatg attttgatgc
     4201 agtgatcaat gtcaacttaa aaggaacatg gctgatgtgc cgtgaagctg cgatacgaat
     4261 gaagcaacag aagtatggct gtattgtgaa cattgcttcc cgtgcatggc ttggtaatgc
     4321 gggacaaacc aactactccg cttcaaaagc cggtatcatc gggcttacac gttccttggc
     4381 attggaaatg ggaagacata atgtttcagt taatgccatt gctcccggtt taattgacac
     4441 accacttacc caaaagttag ataaagatgt tttggataaa ctgattcagg ctcaaccctc
     4501 acgaagcatg ggcaagcctg aagatattgc taatgtggtt tcatttcttg cttcagaaaa
     4561 aacaagtttc atgaccgggc aaaccattta tgttgatggc ggaaaaagta taggggctgg
     4621 gttataaaat aattgcagta ataaatattg aataatgagg tcaattaaaa taaaggtaaa
     4681 cggaaggcag catgaagtgt tgattgatga acatgaaacc cttgctcatg tgctgcggga
     4741 aaaaattgaa ttgacaggca caaagattgg ctgcgaacag ggaagttgcg gagcctgcac
     4801 tgttctgctg aatgatgaac cggtgctcag ttgtattaca ccagccatgc gttgtgatga
     4861 gttgtctgtt accaccattg aaggcgtttc tgaaaatggt gaattgcatt tgcttcaaca
     4921 aaagtttgta gagaaaggcg ccattcaatg cggcttctgc actccgggaa tggtgatgac
     4981 ctcattggac tttgtaagga aaaatcccca tgcgaccgtt gatgaaatca aagaagcatt
     5041 atcgggaaat ctttgccgct gcacgggtta caaaaaaata attgaagccg ttgctgaaac
     5101 catccgtccg caacagcatc cggctgaagc aaatggtagc aagccgcaca acggcagaat
     5161 cgttggaacg ggaatgcctt acattgaagc agctaaaaaa gtgcagggca aagcggaata
     5221 tgccgatgac attcgtttga aaaatgtatt gcattgcagt tttttaagaa gtccttatcc
     5281 acatgcaaag attgagcaga ttgacacaag tgctgcagca aaactggaag gtgtaaagta
     5341 tattctcacc ggagttgaac tgccggaaaa attcggagtg ctgcccattt cgcaggatga
     5401 aacagcgttg gcaattgata aggtaagata cattggcgaa gtggtggctg cagtggctgc
     5461 cgaaacagaa gccattgcaa aagctgcctg caacctcatt caggtaaaat acaaaccact
     5521 gaaaccgttt tttgaaattg atgaatcact aaacgacatc ggcacggaag aaaaaatcca
     5581 tgagcatgtg aagttcaata acaatgttca caaaaaagca gagttgcgtt ttggaaacca
     5641 ggaagaagga tttcaaagtg cagcatttaa aagcaaaatg aattttgaat ttgaaggtat
     5701 cactcatggt ttcactgagc cacatgccgc cactgcatat tgggacgaaa acggtttgac
     5761 catcattact gccacacagg tgcctcacta tctccacagg gctttgagca aagtattgaa
     5821 gttacctttg agcagggttc gtgttattaa accttatgtt ggcggtggat ttggcggcaa
     5881 gagcgatcct tttccgcatg aaataattgt gtcttacctc gccataaaaa ccgggcgacc
     5941 ggttaaagtg cgtttcagcc gtgaagaagt ttttatcagc aatcacggac ggcatcccac
     6001 taaaatgacg atggaaattg cttcggatga aacaggaaat ttccgtgctc tcgatgctga
     6061 tattgctatt gatggcggtg cttatggaag ttttggtgtg gttactactt attacaatgg
     6121 tgtgttgttg caggcgcctt ataaagttga aaatttcggc ttcaaaactt ttcgtgttta
     6181 caccaataag ccgcaatgcg gtgccatgcg tggacatggc gctgtgaatt cacgctttgc
     6241 cattgaaacg ctgattgatg atttagcgca tcaattaaag atggatccgt gtgagttgcg
     6301 attaaaaaat ttcatgccat ccaatacact cacggcaggt caatatcgca tcacttcaaa
     6361 cggcagtcgt gaatcgttgc ttaaagtgat ggagcaatcg cagtggaaag agagatatgg
     6421 taaactacct ttcggtcatg gacttggagt gggctgtgga ttttttatca gcggcagcgc
     6481 attacccatt atcaggaatg aattgccgca atcggtggtg catttaaaag ttgatttcga
     6541 tggacgggtg ttggttactt ccggtgcaag cgacatcggc cagggaagcg ataccatgct
     6601 tgccatgatt gttgccgaag tgctgggcat ttcgatggat aaaatttttg tgcttgcggc
     6661 agataccttg cttacacctg ttgacctcgg cagttattca agccgtgtaa cttttatggc
     6721 gggcaatgca gcgaagatgg cagcggaaaa tcttaaaaaa gaaattatca actcagtggt
     6781 tgcacgaaaa gaaatttcag cagatgaatt gaaccttgct gatgattgtg tgttttcaaa
     6841 cgatagaaaa attgaactga catggaacga agcaattgat tttaccattg cacatcgtgg
     6901 cgctatcaac gttagcggaa aatatatttc tccaaaactc ggtggcgact ttaaaggctc
     6961 gggtgcagga ttgagtcctt cgtatagctt cggagcggta gttacagaag ttaaagttga
     7021 tccggaaacc ggtgccgtga aagtgttgaa tatttggggc gcacacgatt gcggaaaagc
     7081 aattaatcca ttggcagtgg aaggacaatt ggaaggctcg tggcacatgg gattgggaca
     7141 ggcaatgagc gagcaggtaa aatatttcaa cggcttactg atcaacagta atttcttaga
     7201 ttataaaatt cccacttcgc tcgatacacc gcctatccac accaacatca ttgaaacagc
     7261 agatcccgaa gggccatttg gcgcaaaaga atgtggcgaa ggagccattc acccgataat
     7321 tcctgctgtt gcaaatgcag tgttcgatgc agtgggtgca aggattacaa agttgcccat
     7381 cagccctgaa gatgttttac agaaaattaa aaatctttca cttccaaatg cagcacaggt
     7441 aaatgtgtaa gaataaaaat gagtgacaac aaaaaatatt taaagccggt ttctgttcac
     7501 gaagctataa ccatgagcaa ggtatgtgga gatgatttcc gatacattgc aggcgcaacg
     7561 gatgtgatgg ttaaccgcag gcagggaaat gaagaatctt tttgcctgat tgatttgtgc
     7621 ggcattgatg aactgaagca ggtaacggta aaagaaaatt atctgtcaat cggttcgctg
     7681 gtgaggttag acgatttaaa gaaacatgca gccatcgtaa aagaatttcc tgtgttgata
     7741 gaagcagccg aaactgtggg ttcgcctctc atcaggaaat cagccacatt aggcggaaat
     7801 gttttatgtg agaatcgttg tatttattac aatcaatcag agtggtggcg tgatgctgta
     7861 ggttattgcc tcaaatgcga aggcaatatc tgtattgcaa caggcagcaa aaaactttgc
     7921 tactccgaat ttgtttccga tactgcgcct gcattaatca gcatggatgc agaaattgaa
     7981 atagccgacc atgaaggaga acgcaccctg aaacttgaag acatttatac tggtgatggc
     8041 gtttgcccac gcaaccttaa aaaatcggct gtcatcaaat ctatattgtt gcccttgcaa
     8101 cgtgagtttc attcttcatt tcacaaactg aggcaaaggc aaagcctgga ttttacttcg
     8161 cttacggttg ccgtttccat agataaaaac ggaaaactaa aatccgttct cggtggagta
     8221 gatcccaaac cggttgtggt ggaaggcaac agtgatagta atcttgaaga aataataaaa
     8281 ctggcattaa aaggctcccg agccattgac aacgaaatgt tctcacgcaa ttaccgcaga
     8341 tccatgatca gtgtttttct gaaacgcagt tttgagaagt tgataggatg acgcgattat
     8401 gatttctgat aaaagcattg aagtatttgt tttagccgga ggcaagagca gccgcatggg
     8461 tgaagaaaaa ggactgatgt tgctccataa caagcctatg atacaatatg tgctggaaca
     8521 actatccatt ctcaaattac cagtgcaaat aatcgccaat gctgatgatt acaccaaatt
     8581 tggatatgat acgctgaaag atattattgc tgaaaaagga ccgatgggcg gcttgcttac
     8641 agcttttcat tatgccaaat cagattgtgt tttgcttatc agttgcgata tgcctttcat
     8701 cacatcagaa atagtcagca aactaatttt tgaggtgaag gatgatgaaa tcactgcggc
     8761 ttatgttcaa aacgaaccca atccattgtt tgcagtctat aaaaaatcac tgaaaaacga
     8821 agtggagaaa cgaatagcag caggaaactt acaaatgaag catctcataa aatcgttccg
     8881 ccacaaaagg gtggaaatgg atgtatattt gaatcggaat cctaacttgt ttatcaatct
     8941 gaatagtaag caggatgtaa ataatattac agatgtatgg aactgaaagg aattaagatt
     9001 ttagcgtttg gaatgattgc tgaaaaaata aacgcgagcg aattttattt gaaagaagtg
     9061 agcaatacca atgaactgct gagttcgctg aacagtttat accctcaact taaagaaatg
     9121 aagtttacaa tttcagtaaa cagaagaatt gtaaataaca acgaaccttt aaatgcggac
     9181 agcgaagtgg cattgcttcc gccattttca ggaggataat taaaccataa tgagcaggtt
     9241 tgagaggcaa ataaatttat ccggtttcgg gccaaaggca cagcaacaac tgcaaaaagc
     9301 aaaagtgctg gtgataggag caggcggatt gggatgtcct gtgctgttgt atcttgacgc
     9361 agcaggagtt ggcacattag ccatttgtga cggtgatgtg gttgccgctt ctaaccttaa
     9421 ccgtcaggta attttttcgg aatcggatat cactaaatca aaagcagaag ttgcggggat
     9481 ttacctgaag caaaaataca gcgatattaa tatcgagatt atcaataaat ttttaactgt
     9541 tgaaaatacg cttcaaattt tcaacgctta tgatttgata attgatggta gcgataattt
     9601 tcaaacgcgc tatatggtta acgatgcctg tgtgctgctg aataaaccac ttgtgtttgg
     9661 ggcagtgtat caaaacgaag gtcaggtggc agtgtttaat gtaagtgatg ataatgccgt
     9721 ctcttcaaat taccgtgatg tatttccgaa tccgccttct gaaaacgaaa ttctaaactg
     9781 caatgaaact ggtgttttgg gtgtattgcc cggcattatt ggtgtaatga tggcttcgga
     9841 agcgataaaa ttaataacgg gatttggcaa agctttgata aataaaatgc tttactacaa
     9901 cctgcttacc aacaccactt acgaaacaga aattgcaccc aacgctgttg caaaaagtat
     9961 tattccaaaa accccagaag acttttacaa aactgattac accctactat gcggcaattt
    10021 gccatccatt gagtggagtg aagcgttgtt attgaaaaat aatactcccg gcaaagttat
    10081 tttgcttgat gtgagggaaa cacacgaaca gccgaagttg gatggaatag aaacaatgaa
    10141 tattccgctg cagtcacttt ctgaaaatat tgagccatta aatacagcag aaaatatttt
    10201 agttttttgt cagtcgggaa taagaagcag aaaagctgtt gcagtgttac gcagcaaatt
    10261 cctacagaaa aatatttatt ctgttgccgg aggaatttca aaacttaatt caaccatcaa
    10321 cgcttagcaa acatggaacg taaaccaaaa cagattttta tacagggggc catttcttcc
    10381 tcaaaaattg cagactccat tcaaaagcac agcagtaaaa cagatattgg cgcacacagt
    10441 atttttttgg ggcaggtaag aaccgatgaa aagccggaag gaatcattca ggcgattgaa
    10501 tacacaactt acgaagagat ggctttacag aaagcatttg agatacgcga agatattttt
    10561 gcaaaatacc cgctcacctg tttacacatc tatcatagtt tgggtgttgt aaaaaccgga
    10621 gaaatctgtt tgtttgtttt tacttcttca aaaaaccgga aagcagcaac agaagcttgc
    10681 aatgagatgg tggaacgaat caaaaacgaa ttgcccgttt gggggaaaga aattattgat
    10741 gagaaaaaat ctttgtggaa agaaaacagt taaagaagta tgataaacat tacgcataaa
    10801 aacagcacgc accgcactgc catagcacag gcaattgtga aagtgagtaa agaggaaacg
    10861 ataactgccg ttaaagaaaa taaagtgccc aaaggcaatg tgtttgaaat gtcgaaaact
    10921 gcgggattgt ttgccgccaa acgcacatcc gatatgattc ccgattgcca tcctcttccc
    10981 attgaatatg cacacatcac acatgagata aacgggttgg aaattttaat ccgcgtggaa
    11041 attcaaacca tttacaaaac cggagtagaa gtagaagcca tgcatgctgc ttctgttgtt
    11101 gcacttacca tttatgatat gcttaaacct attgataaaa atatttccat tgaacaaata
    11161 aaactgattg aaaagaaagg cgggaaaagc gatttcatta aaaaggaaat aagcggaata
    11221 aaggcagccg ttatagtttg ctccgacagt atttcaaaag gcacaaaaga agacaaggcg
    11281 ggtaaagcaa ttattgaaaa actaaaatct tgtaaagttg atattgccga ttacattatc
    11341 attcctgacg aaaaaaatat tattcaggaa aaagtaagag catattgttc tcagcagatt
    11401 caactggtaa ttcttacggg cggcacagga ttatcgccac gcgatattac tccggaagca
    11461 ttagacagcg ttatcgaaaa acggataccc ggcattgaag aagccatccg cacgtatggt
    11521 caggaacgaa caccatactc tatgctatcg cgcagtattg caggattagt aggcaacacc
    11581 cttgtattgg ctttgcccgg ctccacacgc ggtgctgccg aaagtatgga agccgttttc
    11641 cctcacatac tccatatttt cagagtgatg aaagatgcga ggcatgattg agaaaatata
    11701 ccttaaagaa aactcttgta atcaagtctg aattacccca acattatatg gtttagtaat
    11761 aggattgtga tttgccattt caatgcccat gctaatcata ttacgagtct ctaatgggtc
    11821 aataattgca tcaacccaaa gtcttgatgc tgcataatat ggtgatgtct gtgagttgta
    11881 tctgtcagta attgttttta gttgtgcttt ttcgtcttct tcagaaattt ctttaccctg
    11941 tgctttaaga gcagccacct gaatttgcaa taatgttttt gctgcttgtg aaccacccat
    12001 cactgctatt tgtcctgtcg gccatgcagc aattaatctt ggatcgtagg ctttgccaca
    12061 cattgcatag ttgcctgcac cataactgtt accaatgatg atggtgaatt taggcacgac
    12121 actgttagcc atagcattta ccaatttagc tccatctttg attatgccac catgttcact
    12181 acgactacca accataaagc ctgttacatc ttgcagaaac actaagggta ttcttcgctg
    12241 attgcagttc atgataaacc ttgctgcctt atctgcactg tcgctgtaga taacaccacc
    12301 aaactgcatt tcgccttttt tacttttcac aaccttgcgc tgattggcaa caattcctac
    12361 agcccatccg tcaatacgtg ctgttccgca taaaatactt tgtccgtact tttctttgta
    12421 ttcatcaaac tcactgttat caactaatcg gttgataact tcatgcatat cgtattgctt
    12481 ggaattttct gccggaatta ttccataaat ttcttttgga tttgctgcag gttcctttga
    12541 ctctgctctc tcaaaacccg ctttgtcaaa tgttccaatt ttagaaaaca aacgtttaat
    12601 atagtcaaga caagatgcat catcaggaaa tttattatct gtaacacctg aaatttcaca
    12661 atgagtagta gctccaccta aagtttcatt atctatatct tcaccaatag cagactttac
    12721 caaatagctt cctgcaagaa acacagaacc tgttttgtca actattaaag cttcatcact
    12781 cataataggt aaataggctc ctcctgcaac acaacttccc ataattgctg caatctgtaa
    12841 tattcccatt gatgacataa ctgcattgtt tctgaatatg cgaccgaaat gctctttgtc
    12901 cggaaatatt tcatcttgca taggaagaaa tacacctgca ctgtcaacaa gataaacaat
    12961 aggtaactta ttttccatgc tgatttcctg tgcacgtaaa ttcttttttg ctgtcatcgg
    13021 aaaccatgca cctgccttta cagtagcatc attagctaca acgatacatt gttttcctga
    13081 aatatatcca atcacaataa caacacctgc agaaggacaa ccaccttgtt cggcatacat
    13141 gccatctgct gcaaaagcac ctacttcaaa ttgtggttta tcggtatcca acaaatacgc
    13201 aattcgttca cgtgccagca ttttgttttt ttctttttgc ttagctgcat ttttctttcc
    13261 accaccttca taaattttat ttaatcgctc tgctaaacga atcaaatgtg atttataagc
    13321 atcttcattg atattaaatt caagattaac tttctctgcc ataattagta ttcaatagcc
    13381 tgcaaaaata acttaaaaaa gtcaacttta ctgaatgtac tttaggcaag tctatgactt
    13441 ttgtttattg ttcttatctg ccttcttagt attgtaattc catggacagt ggcggcaacc
    13501 gctttgacaa caatatcctc tttttagatg atatttctca gtaaatacaa tatacccttc
    13561 aggagtcagg tagtattcat ctttttcaaa cttgtctgta aaagccattt cgtttgcaaa
    13621 attcaacaat atacagattg ttttcaaata atattttatc gtatcgtatt gagtgatgaa
    13681 attaaggatg tatatactca aagcaattca tgtataactc tttagatttg caaaaatgaa
    13741 tagtccaatt attgatgaag taaatctcaa cgactttcag ttggcacata aaggagtgag
    13801 tttacatgta ctcagactcg atttggatga tgtcataaca ggaggcaata agtcttttaa
    13861 gttaaaatac aatcttcaag cagcaaaaga aaaggggagg agcataatag ttacattcgg
    13921 tggtgctttc agcaatcata ttgcggcaac tgcacgtacc tgcaaattac aaaacttaaa
    13981 agctgtgggt attatcagag gtgaaattgt tacgccaatg aataacactt taagtcgtgc
    14041 tgttaatgat ggaatgacgt taatacctgt cagtcgtcaa tggtatagac aaattcatga
    14101 taaagaattt tataaaagta atttagtaaa gtgtttacta attgcaaaca atataaatcc
    14161 tgatgcttgt tatgtagttc ctgagggagg aaataatgcc gaaggcttta aaggttgttt
    14221 ggaaataatg aataatataa atgctgatta tgatacagtt gtatgtgctg ttggaacagg
    14281 aactactctt gcaggaattg ctgcatcact accccaaaat aaaaacgtac atggtattgc
    14341 cgtagttctg gctgccgata ctctgactaa aaacatacaa ttacacttag ctgaaagtaa
    14401 ttcaaatgca aagtatattg ttcaccaaca atactccttt ggtggatttg gtaaaagcaa
    14461 tgcagaactt aaaacatttt gtgagttatt tacacaaaaa actaatattc ttattgagcc
    14521 tgtttacacc ggtaaattgt tttatgcaat acttgatatg cttgcaaaag ataagtttaa
    14581 tccgggaaca aaaattcttg ctattcacac cggtggttgt cagtatttga ttgataaata
    14641 aaaaaaggca atccgaagac tgccttttca aaatatatta gactttataa aacttaatcc
    14701 aaagttattt ttttggtaat cacgtcagag ttgtaagtta tttttactaa tacaacacct
    14761 ttgttcagtg atagtgatgc cttttctatt cgctgattaa atgcatttgg atttttctca
    14821 gatgctaaaa gactgccttt aatatcataa acttcaatag aacgcattgg ctctttgctg
    14881 gtagaaacca cagtaaaata gctttttgaa ggactattat agattgttac atctgaactg
    14941 tttgtagaaa cttctttaac agatgttggc aggttacggc agaaaacgat tctgggattt
    15001 acgtagttca taatggtatc aatgtaagcc aaagcataag tcttgctcat agttgggttt
    15061 gttaacatag aattaccata acaagtatct ccgcgtgttg actgataacc cattgccata
    15121 gcaagattac gtgttgattg taaatcgtac cattcccaag gaccggcatg tccaagttgt
    15181 gggtttaccg gattaatacc ttgctgaagt gggaacaatc cttcattgcc attattaata
    15241 ctgttagcac gaagtgtata aggatccgta aaccctgcat tagcaaaaca tgcattattt
    15301 cctaattgat tagcccattt aataatttca cctgaaccta ttacatcaac aacagcttgt
    15361 ggtggagtag tgggtacata aacaattcca acaccgtaag gagcaaatgg atcgtcaaca
    15421 caatgaattc caatcatagg agcatcaccg gcagcaatcc atgaactatc acccatggca
    15481 ccacctaaat tcactacaaa ctgaacagca tttgaatatc cgggattatt ttcattgtta
    15541 tagggtgcca caccaccaat gccgtcaaaa tcacccaatg cagtttgatt tacataaggc
    15601 tgtccggcat aaatgccata ggttggtgca tcagtgctgc tgataaactt aggcagcgta
    15661 atttcagacg gactgtttag cactgcatag ttcaatgcaa tatatccgcc tgttccctga
    15721 ccaacgagat aaaaattatt cacatcaatt ttataaacat tattgtttgt tgcagcatct
    15781 tttctgaaat atcgcacaca tgactttgca tcttgtattg aacgataaac tgcattcagc
    15841 aaggtacctc tacgaatgtc aagtccattg tttggattcg tatttgtagg atcccatcct
    15901 aagcggtaat ccatatttgc aacaacatag cctctgcgag caaactgctt gcaaatttct
    15961 actgctgcac tgtcatttct tgatccggtt gcatttccat tcacaataat aggaatataa
    16021 cttcctgtat gtaaataaat aatcaacggt ctttcagtca ttgggtcagc agcacctgca
    16081 ggctcataaa tatccatttt taaatctttt aaaactggtg ttcctgtaag gacttcgtag
    16141 ttctgaccat aaactacatt gggggtaaca ttaacagaag ggaatacttc agttaaaaat
    16201 cgttgtgcct gtgcagtata tgcaaacatg ctgaacatgc ccaatactgc taatttttgt
    16261 aaatgttttc tcatcttaat atggttttat ggacgtaaaa ataataacaa tcttcaaata
    16321 aaaagcaatt actgaaaact taatctatca ggtcaataaa ttctgataaa atcattgctg
    16381 ttgcacccca aataaccagg ttgccaattc tgaaagcagg agctgttatt aagcctcttg
    16441 tagtctgatg ttgcagtcct tgtataattg ttttattttt aatcaaacta tgcaatggga
    16501 tttcaaaaat ttctttcact tcattttgat ttgctaagaa aactgatgtt tgaatctgag
    16561 tggcaataaa aggatatacc atgtaattgc tcggtggaat atatattctt gaaagctgac
    16621 ctatgatgtt gatgctctta tcagcaattc caatttcttc ttccagttcg cgggttgcag
    16681 ttagagtcaa actctcatca ggctctgcct ttccaccagg gaaagcaatt tgacccgaat
    16741 gcactttgcc atcttctgca cgctgaatca ttacaagatg cggaatgtta tttttattat
    16801 tgacaagcaa cataacactg cctatgcgat aattcccttc aggctcataa acaggacggt
    16861 taacagatgc taattttaaa tgagaatttc tgccgggcaa atctttcagt cttccggaaa
    16921 taaaaacttc aaagtctgtt atgttatcaa gcggtgacaa ctctttctcc ttttttgcgt
    16981 tcaccaatct gctgacgcca catggcataa taaagacctt tctcttcgag aagcgtttca
    17041 tgtctgcctt cttcaatctt atgtcctttt tccaatacaa aaatatggtc agcatgcatg
    17101 atggttgaaa gcctgtgtgc aatcaatatg gtaatgagat ttttattttt gttgatagat
    17161 tttatagttt ctgtaatctc ttcttcagta atggaatcaa gtgcagaagt agcctcatcc
    17221 aaaaccaaca gattaggatt gcgcagcaaa gctcttgcaa tagaaagtct ttgcttttca
    17281 cctcctgaca ctttcacacc gccttcgcca ataactgtgt tcaggccatt ttctgctctt
    17341 gccaaaagag agtggcacga tgccatattc aatgcattca atacttgatc atcatgggca
    17401 tgtggattta cgaataaaag attttctctg atagttcctg caaacaattg agtatcctgt
    17461 gtcacaaaac caatctgctc tctcaactca ttcagattaa tgttgttggc atcaatccca
    17521 ttgtaaagaa tttttccctc tgccggatga tacaagccca ctagtaattt tactaaagta
    17581 cttttaccac tgcccgaagg accaacaaag gcaattgtct cgccacgtct ggctttaaaa
    17641 ctgatattgt taacagcatt gccatgtgcg gatatatgtt taaaagaaac actactaaat
    17701 tcaaatgttt caattgcatt caactgaaga ggtgattgtg gtttgtattc aagtggcatt
    17761 tccattatct cactaaaatt attcagtgaa gcctgtgctt cgcggtaaac agcaataacg
    17821 ttacccaact cctgtagcgg gccgaaaata aaaaacgaat aaaacattaa tgtcatcatc
    17881 ttaccaggtg aaatttcatc cttaaaaatc agatagtaca acaaaaaaac aaacaatgtt
    17941 ctcagaaaat tcaccgttgt gccctgtata aaactaaggc tgcggataaa cttcactttc
    18001 ttcaattcca gattcagaat tttatctgtc actgtgttca gtctgtttat ttcctgattt
    18061 gccagcccca gactcttcac taactcaata tttctgagcg actctgttgt tgcacctgct
    18121 aatgcagtag tctcacccaa tattttttta gaaataacct taatcttttt gctgagcata
    18181 ttgctcacca cagccaaaac cggcacagta agcagaaaaa caggtgcaat gctccaatgt
    18241 acatttatag cataagccat cacaaaaatt attccaacca cactctgaaa aagaatactg
    18301 ataaatgcat tgataaactt ctcgctgtcg gcacgcacct tttgtaactt acctagggtt
    18361 tcaccgctgc gctgatcctc aaaaacctga tagggcaatt ccagcgaatg tttaatgcca
    18421 tcgctataca tctctgcacc ggtgcgttgc acaatcacat ttacaaaata atcctgaaaa
    18481 ttttttgcaa tacgcgacac caaagctact cccattgaag ctaaaaggaa ccaaataata
    18541 tcctgaaaaa atttaggaaa atcattactg tagttcgata catgcatagc aaatcgatcc
    18601 agcattttac ccgcaatcaa cggatccaat aaagaaaaac attgattaac agccgccagc
    18661 aacaaagcta ataataccaa cttccagtgg cgttttaaat aagttactaa tattttcaaa
    18721 cttcttgatt tgttaggagt ttcaaaaata gtggtttagg aagtaggaaa tgaatcagta
    18781 gtttaatttg tattaatatt gagtgggagg gttaatattt aggtaaggtt ttattataaa
    18841 aatgtgtcaa ttttaaaaag tcaacttata attcttttaa aaataaaaat tcattactgg
    18901 cttattttat ctttttattt ttatttttga attgaaaaat agttttaatt ctgatatgta
    18961 tcagaacaaa acaatatgct cgatagcgat tggacttact atctcattga caattctatg
    19021 caacagctaa ttgatacata gaaaataatt aaaagacaca gtttatttta tatgcaaaaa
    19081 atcgttaaac tatatgaatg gacaacctcc ttacctatta ttacatgttt actatatttt
    19141 tcaggtttat tagataacaa tattgttttg caaatattgg caggaatatt actgctgttt
    19201 tgtgtaatgt cagctgtaca tcattcagaa atcgttgctc atcgtgtggg cgaaccttac
    19261 ggaactatta tcctggcagt atccattaca attattgaag ttgctataat aatttcttta
    19321 atgatgaccg gtggtaaaga atatgcttca ttcgccagag acactgttta tgctgctgtt
    19381 atgttgattc ttaatggaat tataggactt tcattgtttc ttggcggaag aaaatatcat
    19441 acacaaacat tttctcctca ttcagtaaag attgcactgg tagcacttgt gtctattgta
    19501 acattcacaa tgatattacc cacctttaca agtagtatta ttggcccata ttacacagaa
    19561 cgacaattgt tatttgaaat aattgcgtgc ttaattattt atatagcttt tatagcagct
    19621 caaacaatta gatataggga atattttatc actgaagcta atcccgatgt agaagtcact
    19681 cacaataata tctctaattc ctcatttatt attagtttaa tttttctaat gatttgtctt
    19741 gttatagttg tgttacttgc aaaatcactt tccaatccca tagaaaattt attaacttct
    19801 tatggattgc cacgtagttt agtcggaatt gttattgcag ctgtaatttt attgcctgaa
    19861 ggaattgcag ccgtttcatc ggccagaaaa aataaattac aaaacagttt gaacttagct
    19921 ttaggttcgg cacttgcaag tataggattg acaattcctg ctgtttctct tgcaagctac
    19981 atttttgacc tgccgcttat tttaggtctc gaattattgc ctataatatt attagccatt
    20041 tctattttta ctgtaatgct ttcactcatt ggaggacgta gtaatattgt ttatggtgtc
    20101 gttttgttag tgaatttatt tgcgtacgta tttttaacaa ttaatccata actgtaattt
    20161 taacacaata ttttactata gtaagttggt tttaatatct gaatcagatt tttttatcct
    20221 atcaaagtat caatgatttt acattggtgg gactctgtgg aatagcaagc aatataactt
    20281 acaaaacatt aaaaataatc taatcatttc attgccttat cattttttat tactattgta
    20341 aatggatagt tttaattcca ttcgacataa taaagttagt tatctttggt aactgtcaat
    20401 aatagagatt tagctaaatg gagaccttaa agaaaagaat aaacaataat gttaaccaaa
    20461 catacacatg aaaaagcttc tacttcttct atttgtaact tcacagatta cattttgcaa
    20521 agcacaacaa ttttcttttc aaatgttttt ttcagatgcc attggtaata aagacaccat
    20581 tactttggga tatgatttaa ctgcaactga tagtattgat actgcatttg gtgaaacaaa
    20641 tatcatttca gttcctttag acacaaatct ggatgttcgc attacaaatg catggtttac
    20701 ttatgcagac gctgtttact tccatttaaa aaagcaaatt gttttctatg attgttctct
    20761 acataattta aatattcaac caattaatat tgttactcaa cactggccgg ttacaatgac
    20821 atggaataat agtatattca atgactcatg taataatgga agtgttttta caggtattaa
    20881 tcctgctggt tggtgggaca caggctgccc aagcggctta ttacaacagg tttttctagg
    20941 tgatgactca atcaccttca attctaactt tgatttaggt tggttcggtt atatcaattc
    21001 aaatggtgat tcaattccgg ttttttggca agcatttggc gattcaacaa ttcttactac
    21061 cggtattaat gaacttccgc aacaatgtat ttcagtaaaa gtatttccga atcccggttc
    21121 tacttacatc actgttcaag caaacaattc attcggacag ttaagcctga tttcaatctt
    21181 aaattcttat ggacaaattg tactcaaaac acaacagata agtaggattg atataagtga
    21241 gctctccgat ggaatttatt ttattaaaac agaaaatact aagggagaat ttacaacaag
    21301 taaattttta aaactctgag gtaataaata tattcaactt caacagatca tatttccccc
    21361 aaaattcata ttatcttttc tactttgaaa gattgaatta ctaaattcaa tttcgagtat
    21421 tcagcaagta ttcagaatat tacttatcct atcaataaat agaaaaagta ttattaggca
    21481 attcaaaaca cttactgaaa aaggatgtac caagattgcg caagaataca tttccgaaca
    21541 aaacattcta ctttctttta ctattcaatc ctttcaagtc ctgtttgata aacaagagta
    21601 taagaaacta cactatatgt attatttcca ttttgcaatt tgcccaaact taactgtacg
    21661 tgaattccaa atcttactct gtattgggtt gtaattccac gaagatacca tagctacaat
    21721 ttttaaatct gataatttat agccgacaac cgggaacgaa tagctcatta cggtttccgt
    21781 tgaagtaaac gtgatatcat caccaagtgc gttagttggc attaatcgaa caacttgctg
    21841 gtgaaaaaaa tttcccacac tgcctaattg gttttgtgca agcacattat cttctgttac
    21901 aaaaattgtt aaacgcaaat tatcgttaat gtgagaatta tgacctatat gtacctccac
    21961 tctgccggaa tcattttcaa gctttgaaat taaagcaata cccaaatcat gtgtttctgc
    22021 cagacgcgat gttacattgc tttgccactt tgtgtaatct acataatatg taccagtact
    22081 aggttgaaca accctaccca cagttgcaca aggaaaaccg aacatcgctt gccaataatg
    22141 gaatcttaca gtgtcatatt taactatttc aagccaatca acatcgtgta tggaaaccac
    22201 atccacttta ccgccattaa tggctgaatc tttaatagat tgaagaatat gagccccatt
    22261 aacacaacct ggacaccatt cgcccgtcag ttcttcaatc aaaactcttt gttcaaaaac
    22321 agaaggcata gtaccgagtg agtttgtaat actagtcgaa tctggcacat tcggactaac
    22381 ttcatcagta ttatcatttt tacggcatgc gttaaataat agaaaaagta gtatagcagt
    22441 taaaaaaata aattgtttct tcatgacatg atatttattc agtaagtgtt aaaaatgcat
    22501 taaaagtttc acattttcaa aaataaattt tcccattatc ctcatattcg cttcagatcc
    22561 ccacgacaaa acagagtttg aagctttggg gaggtaacta acattcaaca actttctaaa
    22621 ctccaaccct tactttcaca atcttatagc tattagcatt tgacttgtgc tttttcaacc
    22681 caaaattctc aacccatttt tctcttgcgc taaaagccgt aaaaatcaaa accgccatta
    22741 tgcttagcac aacagcggtt tatattttat taagtgtatt taatttaact aatcaacaaa
    22801 tcaccaatta tcaaatcaaa caactttcta aactttcaaa ccttctaaac tttccaactt
    22861 agtttagtag cgataataat caggcttata aggtcctgca actggaacgc caatataatc
    22921 tgcctggctt tgcgttaatg tttccaattc aacaccaatt ttacttaggt gcagacgtgc
    22981 aactttctca tcaagatgct ttggaagtgt gtaaactttg ttctcatatt tatcggtatg
    23041 attccacaac tcaagctgtg ccagcgtctg attggtgaac gaattactca tcacaaatga
    23101 tgggtgaccg gtagcacaac caaggtttac caagcgacct tctgcaagta caataatgtc
    23161 tttaccatca agcgtatatt tatcaacttg tggcttgata gtatctttag aattaccata
    23221 atttttattc agccatgcca tatcaatctc attatcgaag tgaccgatat tacaaacaac
    23281 tgcattatgc ttcattgctt taaaatgttt gtcaacaata atgttatgat ttcctgttgc
    23341 agtaacaata atgtctgctt caggaatagc attgtccatc gtttttactt cataaccttc
    23401 cattgcagcc tgaagtgcgc aaattggatc aatctcagtt acaattacac gaacaccggc
    23461 actgcttaac gattctgcag aaccttttcc aacatcacca taacctgcaa ctactgcaac
    23521 ttttccggca agcatcaggt cagtagctct gcgaattgca tcttgcaacg attcgcgaca
    23581 gccatattta ttatcaaact tcgacttggt aactgagtcg ttaatgttaa tggcaggaat
    23641 aggtaatgtg cctttttgca tacgctcata caagcggtga acacctgtag tagtttcttc
    23701 gcttaatcct ttaatatctt ttatcagttc aggaaatttg tcaaacacca tgttggta
//
LOCUS       DAOOOI010000031        23285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_411_length_23285_cov_21.060181, whole genome shotgun sequence.
ACCESSION   DAOOOI010000031 DAOOOI010000000
VERSION     DAOOOI010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 23285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23285
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_411_length_23285_cov_21.060181"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1445)
                     /locus_tag="PKX59_05690"
     CDS             complement(81..1445)
                     /locus_tag="PKX59_05690"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOZ90394.1"
                     /translation="MLSLNKVKNAINSGLFVLFLSFSFSTNSQAQDTLKSENNFDIRG
                     QLRTRFELRDGAFRPLTEKEQPAALVSERIRLNFDYSYRDLLTVKISPQAVGIWGQAN
                     MVQGAEYSGNHFALFEAWSKINLSPSVNIKFGRQIISLDDERFFGELDWAQGGRAHDA
                     LAIDIHKKSFDLRGYFAFNQNYKVMYANNLNNASGNLYSTTDAFPYKWMQTIWANIPI
                     GKYYRFTLLANNLGYQQATYNTIDTNTIFTQTLGLNNYYKKNKIDIHVAAYYQYCDNS
                     QTINTNAFLITANAALQINNKLNLGLGSDYVSGNDVGKTNNINHAFNPYFHTGHKFYG
                     NMDYYYAGNAHKNAGISDSYLKVNYKSTGKLALNATLHQFISPNKISDGYSKNYNSNL
                     GQELDLSFSLKVNTFATFTGGYSFYLATSTLKYLKNTPGANDYQQWLWLSLNVTPHFL
                     KTNF"
     gene            1797..2249
                     /locus_tag="PKX59_05695"
     CDS             1797..2249
                     /locus_tag="PKX59_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOZ90395.1"
                     /translation="MNLEQKIMAELKTAMLAKNEAALRALRAVKSAIILAKTSGSNVE
                     LSEADEMKLLQKLVKQRKESLDIFTQQNRADLAKNEADEIAVIEKFLPAMMSADEVKE
                     VLKGIIATMGATQPSDMGKVMGAASKQLAGKADNKMISELVKNLLSGG"
     gene            complement(2250..6887)
                     /locus_tag="PKX59_05700"
     CDS             complement(2250..6887)
                     /locus_tag="PKX59_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOZ90396.1"
                     /translation="MKTFSKLINTVFIFAVTVFVAQAQSPTNQDCPNAIPICNTTYST
                     VISYSGTGNIPNEINNGSSCLLSGERNDVWYTFTTPFFGNLNFTITPNNPADDYDWAV
                     YNLTTATCADIFTNPSIEVSCNYAPNLGCNGTTGPNNQTAGPCGGQNNPIIPVTPGQT
                     YVINVSNFSSTQSGYTIDFTASTGSIFDVFPPFLLTTTPLTCGSNSLTMTFNENVTCN
                     SVQATDFVLTGPGGPYMITAVNSAGCTSGADYSKVYTVTFTPAISVAGIYNFAVVSPV
                     QDLCGNLISVPTNKSININGATVSAAHTDVSCFGDNDGSIILTPGGMGPFTYQWSPNV
                     SSSNTATNVTAGTYTVIVSPSNGACSALATVTVTEPPDLVLQNQNIVSAVCGAANGSI
                     ATTIAGGAAPYQYQWSPSGGAGANATALLPGNYTLTVIDNNNCDFTQTFNVPDNNNIN
                     ASVNTIQNVSCKNGTDGEITLSVTGVSASASYQWSNGATTKDLLNIPAGSYTVTVTDG
                     NCQTTLSNLTIIEPLTSVMSSPLVTSTSCGLNNGAITVNASGGTGALSYQWTPSGTGA
                     LSQNLASGTYTVTITDANGCTAKRTVAVAASTSPVATVNYIKQMVSCYNGSNGSASIS
                     VNSGTAPYSYLWSAGLGTTNSNNALSTGNYNVTVTDQAGCTTNATVTILNAPQLTLTS
                     GTVQHVKCFGQNTGSATFNGSGGTGTLTYLWSPAGGNSNSANNLYAGTYTVLLTDANG
                     CTKNSSVTINQPTAVLNSSITPTATNCGLNNGALVTSATGGTTPYTYLWSNGSVNANI
                     SNLASGTYTLTVTDANLCVKTKTASVAASTAPIINVTNLQNLNCANANNGSITINVTS
                     GIQPYSYQWTNNVSNSNSASNLAAGSYVVTVTDNAGCIVTSSNNITSPTAITIQTVSQ
                     QNVKCFGNNTGNINVASSGGTGTLSYLWSNGNTTSSITTLTMGNYTVTVTDQNNCTKQ
                     QSYTITQPASALSSATTPVGTSCGLNNGSALAVPTGGTSPYKFLWSNGLNTAQINNLA
                     SGNYTVTVSDFYNCSLTQSTIIQPSTAVTIQSTQQANVDCNGGSNGTASITATGGIAP
                     YNYLWTGGQTTSSVQNLVAGNYQVTVTDNAGCTVLNSFTITQPTAIVVSMPAAQTVCS
                     LQSSTAIASPSGGTAPYQFLWSNGQTNDTLYATPSANTTFTVTVTDNNGCTSVASAII
                     NVYPQLALNTFNPYSVCQNTSLSFTANVSGGNGQYQYNWNNGASAQQTFTQTYSSSTS
                     VNITATDGCNYSTSAIIPINVVPTPIVTFTAENTTGCIPLTVNVTDNSQSIAGSVYNW
                     NTSNGTLSQQGNNYQITYATSGLYYPILTISTPAPENCTATYTYTDGINALDRPVADF
                     SFAPEEPTMLNPEVTFKNQSQFATVYSWSFGDGIISSEINPINRYTTIGTYEVTLVSS
                     NTYCEDTITKTIEVTDQFSYYVPNSFSPNADGRNDVFTSQGTNIVSERMVVYNRWGMK
                     VFESSVFPFNWNGRMQNTGDELQQGIYVYTISITDKFGKGHSYNGTVTLLK"
     gene            7023..7964
                     /gene="mdh"
                     /locus_tag="PKX59_05705"
     CDS             7023..7964
                     /gene="mdh"
                     /locus_tag="PKX59_05705"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOZ90397.1"
                     /translation="MKVTVVGAGNVGATCANVIAHRELVNEVVLLDIKEGTAEGKSLD
                     MWQTAPINLYDTRIKGSTNDYAATAGSDVVVITSGLPRKPGMSRDDLIATNAGIVRSV
                     TENIVKHSPNTIIIVVSNPLDVMTYCAFLTSGFDSKRVFGMAGILDTARYRAFLAEAL
                     NCSPKDIHAVLMGGHGDTMVPLPRYTTVSGIPVTEMIAADKLESIIHRTKVGGGELVN
                     LMGTSAWYAPGAAAAQMVEAIVRDQKRIFPCCAWLQGEYGQKDIYLGVPVKLGKKGIE
                     QIIELKLNTEEKQLLDGSAKAVKEVMNVLDGMQQPVK"
     gene            8118..9296
                     /locus_tag="PKX59_05710"
     CDS             8118..9296
                     /locus_tag="PKX59_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008636177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HOZ90398.1"
                     /translation="MNIILADSNRNDLLPFTFVRPVAEIRCGILTITEKWNRLSGAQC
                     SWLTEKYLSAKYSLAKEKVNCVANASVLIDKALFNEIKTLKTGQALVKGNTVIAVCTE
                     QQQFETLEDVSYNEAFETGLEVNVLKYPWDIFRFNDKAINSDFQLLTEGRKSAGYDSS
                     NTVINEQQVFIEDGAKVMCSIINASTGPVYIGRDAEIMEGCMIRGPFALGEHATLKMG
                     AKIYGATTIGPHCKVGGEVNNSVIFGYSNKAHDGFLGNAVIAEWCNLGADTNNSNLKN
                     NYGMVKAYNYSKKDYINTGLQFCGLMMGDHSKAAINTMFNTGTVTGICANIFGSGFPP
                     KYIPSFAWGGFDDESVFQLDKALELAAEVYKRRELIFDKKEQDIINHVNMLELILPKN
                     "
     gene            9368..11848
                     /gene="lon"
                     /locus_tag="PKX59_05715"
     CDS             9368..11848
                     /gene="lon"
                     /locus_tag="PKX59_05715"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOZ90399.1"
                     /translation="MFNPEDIKHTLIGGINDDEPELIPLLSSEDEEQLNKESIPDELS
                     ILPLRNTVLFPGVVIPITVGRDKSIKLIKDAYKSNKIIGVVAQKDTNIEDPGFNDLNK
                     IGTVASVIKMLRLPDGNTTIIIQGKRRFSLLEMTQSEPYLRAKVEPLNDIVAALKEKE
                     FKATVDSLKDISLQIIQHSPQIPSEAGFAVRNIESPAFLINFISSNMQLEVSKKQDLL
                     EMTDIKQRALKVLEYLTQELQMLELKSQIQNKVKVDLDKQQREYFLNQQLKTIQEELG
                     GNTPDQELQQLKSRAKDKKWNKHVAEIFNKEIERLGRINPAAAEYSVTLNYLDLLLEL
                     PWGEFTEDNFNLKHAQKVLDKDHYGLEKVKTRILEYLAVLKLKKDMKSPIMCLYGPPG
                     VGKTSLGKSVAKALGRKYVRVSLGGLRDEAEIRGHRKTYIGAMPGRIIQNIKKAKSSN
                     PVFVLDEIDKLTNDFHGDPSSALLEVLDPEQNNAFYDNYIELDYDLSNVLFIATANSL
                     SSIQPALRDRMEIIEINGYTVEEKIQIAVKHLIPKQLEAHGLKKHHATFNNSVIEYII
                     EFYTSESGVRSLERNLASVIRSIAKRVALNEKYDKKIDKDTVEKALGAPIFLRDRFSD
                     NSVAGVVTGLAWTSVGGDILFIETSLSRGKGRLLLTGNLGEVMKESAQIALAYLKAHA
                     ADFGIDYRVFDRWDIHIHVPEGATPKDGPSAGVTMLTSLASAFTQRKVKLKLAMTGEI
                     TLRGKVLPVGGIKEKILAAKRAGITDIILCADNEKDIKDIKSEYLKGLKFHYVSKMGE
                     VIDIALLREKVKNPLNFTISQLERKETV"
     gene            complement(11852..12772)
                     /locus_tag="PKX59_05720"
     CDS             complement(11852..12772)
                     /locus_tag="PKX59_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOZ90400.1"
                     /translation="MKRRLLEYLSLLLNYTRRITLPGFQKIPIYDVGEFFLRELFKGD
                     LQIRARAIAFSFFLAIFPAIIFLFTLIPYVPIAGFQERLLSLLQSMLPYNTYDTAYKT
                     IEEIIVRQNGGLLSFGFILALIVSTNGVFALIEGFNKTIMHEKRKGWKQRLVALYLTV
                     LLATLLIIAILLIIITEVILHYFNNHILNLDQSSVYLLLAGKYLILILLCFTAISSLY
                     FFGPSGGSKKWHFFSPGSILATILIAVTSIAFNFFILNFGQYNKIYGSIGTLIVILIY
                     LNFNCIQLLIGFELNNAIEKAKFKPTVVEK"
     gene            complement(12769..13605)
                     /gene="nadC"
                     /locus_tag="PKX59_05725"
     CDS             complement(12769..13605)
                     /gene="nadC"
                     /locus_tag="PKX59_05725"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOZ90401.1"
                     /translation="MDLDQYISLSLQEDVGPGDYSALSCIPADAQSKANLIIKDKGIL
                     AGIELAEKIFKTVDASLQINIFKKDGQHVDKSEIAFTVEGSVLSILKTERLVLNCMQR
                     MSGIASTTYLYAEQVKHTKAKILDTRKTTPLNRYIEKWAVRIGGGHNHRFGLYDMIMI
                     KDNHVDYAGGIKQAIESANTFRKNRNLNIPIEIETRNLNEVEQVLNTGCVERIMLDNF
                     QPALIKEAVSMISGRFETEASGGIILQNVKDYAETGVDYISVGALTHSFKSMDMSLKA
                     SQ"
     gene            complement(13742..14131)
                     /locus_tag="PKX59_05730"
     CDS             complement(13742..14131)
                     /locus_tag="PKX59_05730"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOZ90402.1"
                     /translation="MKKTIKLLLSVMLIVTVVKAADVFDDIGTAIRSGDARQIARFFN
                     NNVDLTIFNQEEVYSKAQAEMVLKDFFSKNAPKSFTIIHKGVSKEGARYAIGTLTTTQ
                     GQNIRTYFFVKESGGNSSIQELRFEKE"
     gene            14336..14803
                     /locus_tag="PKX59_05735"
     CDS             14336..14803
                     /locus_tag="PKX59_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOZ90403.1"
                     /translation="MQIKLLMIGKNKDSFADAAIAEYASRVERFNSFEVKYLKEAKNN
                     KNLTAAMDAEAENIFKELSPNDFVILLDEKGKEYTSVQFATKIEQLRIKNFKQLVFIA
                     GSAYGFSEKVYARSNELFALSKMTFTHTHARLIFAEQIYRAFTILNNHPYHHI"
     gene            14878..16584
                     /gene="asnB"
                     /locus_tag="PKX59_05740"
     CDS             14878..16584
                     /gene="asnB"
                     /locus_tag="PKX59_05740"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOZ90404.1"
                     /translation="MCGIAGILNFQHKPVDLSVLKQMSDTLSHRGPDGEGIWHNEQKN
                     IGLAHRRLAIIDLSDEARQPMHYLGRYTIVYNGEIYNYLELKADLEKKGYQFVSHSDT
                     EVILAAFNHKREKCLEDFDGMFAFAIWDSKLQQLFLARDRFGEKPLRYYMDSERIVFA
                     SEVKALFKAEVPKKVSKEKMDNYLQNNISIYNTETFFDGIKKLPHGHYAFVQNGNIDI
                     REYYRLQYKNLVSMDDHEYAEEFKRLFFLSINRRLRSDVPVATSFSGGLDSSGIVCVM
                     HQLKEKVNYKLFSARFGDDVKDEGKWMKHITDLLHFPQINKQIEPQEILEDIEKIVYH
                     NEIPIGSTSVTAQYLLYKEVKNHHVKVILDGQGADEMLAGYGHYRFHYLNDLLLRFRV
                     AEYINERRAYNDAYQTSLPIGPWQLTKMFLTKTVKPFEDNTRGYQSFKATLYNDMMMS
                     LQLLLNFSDSNSMAHGVEVRLPFLYHKLIDFAFSLPANQIYRNATTKYVYRNAMNGII
                     PDAILNRKDKVGFAPPQERWLPQFKLPENARLRDMGLTYSDNHWRNYISEVFLKVADS
                     RY"
     gene            complement(16585..17343)
                     /locus_tag="PKX59_05745"
     CDS             complement(16585..17343)
                     /locus_tag="PKX59_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ90405.1"
                     /translation="MPSIFITGATAGFGRAIALKFAQHHWSIIINGRRTDRLRTLEDE
                     IKNLGADVISLPFDVRNEQQVNEAIENLPIKWKQIDALVNNAGLAAGVNLIQDGKTED
                     WNTMIDTNVKGLLYVTKNIIPLMIKQKSGHIINIGSIAGKDAYMKGNVYCATKYAVDA
                     LTKSMRIDLLEHGIKVSAVNPGAAETEFSLVRLKGDVEKANAVYKGFEPLVAEDIAEI
                     VYFVATRPAHVCINDVVVTPTAQANTAHLLRKTN"
     gene            complement(17350..19263)
                     /locus_tag="PKX59_05750"
     CDS             complement(17350..19263)
                     /locus_tag="PKX59_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOZ90406.1"
                     /translation="MNLFRKKTVDEIMKRAGDDASQHGSLAKHLGVRDLTALGIAAII
                     GAGIFSTIGNASAQGGPGVILLFLFTAIACGFTAFAYAEFASLVPVSGSAYTYSYVAF
                     GEFIAWIIGWALIMEYGIGNITVAISWSDYLTGLLDSMGIHLPLWMTMDYLTAAKGFS
                     KAGALMLSGRTLESLDAGLRDAYLAWQGAPTIGPLHIVADLPALLIILIITWIVYRGI
                     KESKNAGNAMVIIKLSVVLLVIAVGLFYINTDNWYPFLPNGIPGVLKGVSAVFFAYIG
                     FDAISTTAEECKNPQRDLPRGMMWAIIICTILYIAIALVLTGMVNYSTLAVGDPLAYV
                     FSVLNLKWMSAIIAVSAIVAMASVLLVFQLGQPRIWMSMSRDGLLPKKFATIHPKYKT
                     PSFASIVTGFVVAVPALFMNLTMVTDLCSIGTLFAFVLVCAGVLKMQITPNAPRGSFN
                     TPYVNAKYIFPLMLILTAALSWSYNKDGVSNFLKNTPQPYSAENLIPSLGADRIKDLV
                     NTVSLKDSAAFAKADNDLANYFSQITTDNYLQKLTDYGLSHKEIYESGLGLFKHKIPT
                     WIFIISCLYLCVLTWKHNLSLIPLLGLVCCLYMMSEMGISNWIGFSIWLAVGLIIYFG
                     YSYKNSKLNIIKK"
     gene            19442..22093
                     /locus_tag="PKX59_05755"
     CDS             19442..22093
                     /locus_tag="PKX59_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOZ90407.1"
                     /translation="MKKFFRISIIVIAVLFAAALVAPFLFKDKIITAIKNAANENLNA
                     TFDFKDLDLSFIRSFPSVYLALNDVSIVGKDAFKDDTLISASSIQVKAEFWKLITSGN
                     TQVKYFGLLQPRIHLIALKDGKVNWDITKPEKESPTKSEESSPFHFSLQKYEITNGYL
                     TYNDEALAMNTKIDNLNHTGKGDFTQDNFLLNTLTSIDKLTYTYAGVSYLYQAKVNLK
                     ADLNIESKTSKYTFADNELSINEFPINFSGFVAMPTDDISMDLKFNSPQTDFKYLMSL
                     VPGAYKENFKDVKSSGKLAFNGFVKGVYSEHTMPGFGINLTVDNGNFKYPSLPASINN
                     VNLKLAVSNPDGEPDHTIINLSQAHAELGAEPIDARLYVTTPVSNATFDAMLKGTINL
                     ANIKNYIPMDEPSLSGIFKSDLSISGNMNAVEQKQFEKIKAAGWMSLTAMNYKPKDGH
                     ITKINEMKLSFNPRTVQLENLDAVVGKSDFRAKGVMDNLFGYYFKKELLKGEFTLNSY
                     LVDLNQFMSDDGNAAATDTTASGVAEVPENVDFTLTTSIGKLLYENKVIEQLKGNVAM
                     RDRTLGLNDVNFKMSGGTVVINGVYQTKERNSPYFNFDLALQNFDIQNTVKTFSTVKK
                     VAAIAERCNGKFSSAFNVSGKMDEKMDPVLSTLNGGGKLTTQTVSLNNFEPINKLADA
                     LKMSQYKQMNLSNVNLSFKFKNGSVFVEPFETSLAGSKAVISGQSGFDQSINYNIDLS
                     IPKALMGSAATGVVNGMFASINKATGASFSMPDPVKVKVNMGGTVQKPEIKTSLADAG
                     KGLVEGVKDKAMEQLDAKKQELEDKAKAEADRLKKEAEEKVKSEADRLKKEAEAKAKA
                     EADRLKKEAEERAKKEAKDKLNNIFGK"
     gene            22163..23263
                     /gene="ychF"
                     /locus_tag="PKX59_05760"
     CDS             22163..23263
                     /gene="ychF"
                     /locus_tag="PKX59_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOZ90408.1"
                     /translation="MGLKCGIVGLPNVGKSTLFNCLSNAKAQAANFPFCTIEPNIGVI
                     TVPDDRLNKLETLVKPQRVVPTTVEIVDIAGLVKGASKGEGLGNQFLGNIRETDAIIH
                     VLRCFDDPNVVHVDGSVNPVRDKETIDFELQIKDLEAIDKKIQRIEKIARVGNDKDAK
                     KAYDVLQVFKKHLESGSAARSAPVQSEDFQYVADIFLLTAKPVLYVCNVDEKSVLTGN
                     KHVDAVREKVKDEKAEVLVIAAAIEAEITQLETFEERQMFLSDLGLTESGVAKLIKAA
                     YRLLQLQTYFTAGEKEVRAWTITQGMTAPQAAGVIHTDFEKGFIRAEVIRYNDFVHFG
                     SEQACKENGKLSVEGKEYVVNDGDVMHFRFNV"
BASE COUNT         7474 a         4052 c         4628 g         7131 t
ORIGIN      
        1 gaattacaac taagcagtgc agtaatcaac agtgtagttg cgcaaattgt gaaatgtttg
       61 tgtttcattt tattattagt ttaaaaattt gtttttaaaa agtgtggtgt tacattcagc
      121 gaaagccata accattgctg atagtcgttt gctcccggag tattttttaa atacttcagt
      181 gttgatgtcg ctaaataaaa agagtatcca ccggtaaagg ttgcaaatgt atttactttt
      241 agtgagaatg ataaatctaa ctcctggcca agattgctgt tgtagttttt gctgtagcca
      301 tcactgattt tatttgggga aataaactga tgtaaagtag cattcaatgc aagtttgccg
      361 gtagatttat aattaacttt taaatagctg tctgatattc ctgcgttttt atgtgcgttg
      421 ccggcatagt agtagtccat gttcccataa aatttatgtc ctgtatgaaa gtatggattg
      481 aatgcatggt tgatattgtt tgtttttcca acatcattgc cactgacata atcacttcct
      541 aaaccaagat tcaacttgtt gttaatttgt aatgcagcat tagctgtgat taaaaaagca
      601 tttgtgttta ttgtttgcga attatcacag tattgataat aggctgcaac atgtatatca
      661 attttatttt tcttgtaata gttgttcaag ccaagcgttt gagtgaatat agtgttagta
      721 tctatagtat tgtaagtcgc ttgttgatat ccaagattgt ttgcaagcaa tgtaaatctg
      781 tagtattttc caattggaat attggcccat attgtctgca tccacttata aggaaatgca
      841 tcggttgtgg aatataaatt acctgatgcg ttattcagat tgtttgcata cattactttg
      901 taattctgat tgaaggcaaa atatccacgt aggtcaaaag actttttgtg tatgtcaatt
      961 gctagagcat catgtgcgcg acctccttgc gcccaatcta attcgccaaa gaaacgttca
     1021 tcatctaaag aaataatctg tcgtccgaat tttatattta cacttgggga tagattgatt
     1081 ttagaccaag cttcaaataa tgcaaaatga ttaccgctgt attctgcacc ttgtaccata
     1141 tttgcctgac cccagattcc aactgcctgt ggtgaaattt ttactgtaag taaatctcta
     1201 taagagtagt caaaattcag acgaattctc tcgctgacca atgctgccgg ctgttctttt
     1261 tctgtgagcg gcctgaaggc gccatcgcgt aattcaaaac gtgttcttag ctgacctcgt
     1321 atgtcaaaat tgttttctga ttttagagta tcctgtgctt gagaatttgt agaaaaagaa
     1381 aagcttaaaa acaggacaaa gaggccacta tttattgcgt tttttacttt attaagtgaa
     1441 agcataaata gtattttaat tccggtgcaa aggaacaatc agactatgga ttgtgtaatg
     1501 attaaggtca tagaatgtat gtgataatta taatgagcac aaatgactta agtagggtta
     1561 tgtccagaag tattgaaagg attattgcta tatattccgc caatttcatc aaaattattt
     1621 ttttgtcttt agctttttca agggcttttg tcaggttgat agtggttgtc tatattaatt
     1681 ataggattga tttctctttt gatttcgtat tttactatct ctacagaaga aaatgtattt
     1741 actcttgaag tgacagattt ttattgaatc attacctttg ttcaaaattt gcaatcatga
     1801 atcttgaaca aaaaataatg gcagagttaa aaactgcaat gcttgctaaa aatgaggctg
     1861 ctttgcgtgc tctacgtgct gttaaatctg ccataatatt agcaaaaacc agcggctcaa
     1921 atgttgaatt atcagaagcc gatgaaatga agttattaca aaagttggtt aagcaacgta
     1981 aagagtcatt agatattttc acacaacaaa accgtgctga ccttgcaaaa aatgaagcag
     2041 acgaaattgc tgtgattgaa aagtttttgc ctgcaatgat gagtgccgat gaagtaaagg
     2101 aagttttgaa aggaataatt gctacaatgg gagcaacgca accatcagac atgggaaagg
     2161 taatgggcgc tgcctctaaa cagcttgcag gcaaagcaga taataaaatg attagtgagc
     2221 ttgtaaaaaa tttgctgagc ggaggctgat tattttaaca gcgtgacagt tccattataa
     2281 gaatgtcctt taccgaactt gtcagtaatg gaaatggtat aaacataaat tccctgctgc
     2341 aattcatcgc ctgtattttg catacggcca ttccagttaa atggaaatac agaagattca
     2401 aatactttca taccccagcg gttgtaaact accattcgct cagaaacaat gttggttccc
     2461 tgcgatgtaa aaacatcgtt tctaccatca gcatttgggc tgaaagaatt aggcacgtaa
     2521 taagagaact gatcagttac ttcaattgtt tttgtaattg tatcttcaca gtatgtgttt
     2581 gaagaaacta atgttacctc ataagttcca attgtagtat atctgtttat tggatttatt
     2641 tctgatgaaa ttattccatc tccaaaactc cagctatata ctgttgcaaa ttgagattga
     2701 tttttaaaag taacttcagg attcagcatt gtaggctctt ccggtgcaaa agaaaaatct
     2761 gcaacaggac gatcaagtgc attaatgccg tcagtgtatg tataagtagc tgtacaattt
     2821 tcaggagcag gtgtgctgat ggtaagtatc ggataatata agcccgatgt tgcataagtt
     2881 atctgatagt tgttgccttg ctgcgaaagt gttccgtttg aagtattcca gttataaacc
     2941 gagccggcta ttgattgcga attgtcagtt acattaactg taagtggaat acatcctgtt
     3001 gtattctcag cagtaaaagt aactatcggt gttggaacta cattaattgg aatgattgca
     3061 gatgttgaat aattacatcc atcagtagca gtaatgttta ccgatgttga tgaagaataa
     3121 gtttgcgtaa aagtttgttg tgcagatgca ccattgttcc agttgtattg atactgtcca
     3181 ttaccgcctg aaacatttgc agtaaaactt aatgaagtat tttgacagac agagtagggg
     3241 ttaaatgtat ttaaagctaa ctgtggataa acattgatga ttgcagaggc cacagaagta
     3301 cagccattat tatcggtaac agttacagta aatgttgtat ttgcagatgg tgttgcatac
     3361 aatgtatcat tagtctgacc attgctccat aaaaactgat atggcgcagt accaccggat
     3421 ggtgatgcaa tagcagttga tgattgtaat gagcaaactg tttgtgcagc aggcatcgaa
     3481 actacaatgg cagtgggttg tgtaatggta aaagaattta atactgtaca gccggcattg
     3541 tcggtaacag ttacctgata atttcctgca actaaatttt gaacagaaga tgttgtctga
     3601 cctcctgtcc ataaataatt atagggtgca ataccaccgg ttgctgtaat cgaagcagtg
     3661 ccattacttc ctccattaca atcaacattg gcttgttgtg tactttgaat tgtaactgcc
     3721 gttgatggtt gtataatggt agattgtgtc agactgcaat tgtaaaaatc agaaacagta
     3781 actgtataat ttccggaagc aagattgttg atttgtgctg tatttaatcc gtttgaccag
     3841 agaaatttat atggagatgt gccaccggtt ggaacagcaa gtgcagaacc gttgttaagc
     3901 ccgcatgaag tgccgacagg tgttgttgca gaagataaag cagaagcagg ttgcgtaatg
     3961 gtgtaagatt gttgttttgt acaattattc tgatcggtta cagtaaccgt ataattaccc
     4021 atagtcaagg ttgttataga cgaagtagta ttaccattcg accataaata agatagtgtt
     4081 cctgttccac cacttgacgc aacgttaata ttccctgtat tgtttccaaa acacttcacg
     4141 ttttgttgac tgacagtttg aatggttatt gcagttggtg aggtgatatt gtttgaagaa
     4201 gtaactatac atcctgcatt gtcagtaact gtaacaacat aacttcctgc agcaagatta
     4261 gaagcactgt tactgtttga aacattattt gtccattgat agctgtaggg ttgaattccg
     4321 ctagtaacat taatggttat gctaccgttg ttggcatttg cgcagtttaa gttttgtaag
     4381 ttagttacat tgataatagg tgctgttgaa gcagcaactg atgcagtttt ggtttttaca
     4441 cacaggtttg catctgtaac tgtaagtgta taagttcctg atgcaagatt tgaaatattt
     4501 gcatttacag atccattcga ccacagatag gtataaggtg ttgtgcctcc cgttgcagaa
     4561 gtaactaatg ctccattatt aagaccacag tttgttgcag ttggtgtaat actgctattt
     4621 agcactgcag taggttgatt aatggtaact gagctattct tagtacatcc atttgcatcg
     4681 gtcaataata cagtataggt gcctgcatat aagttgttgg cagaattgct attcccacct
     4741 gccggtgacc aaaggtaggt gagcgttcca gttccaccag agccattaaa agttgcactg
     4801 cctgtgtttt gtccaaaaca ttttacatgt tgaacggtgc ctgaagttaa tgtcaattgt
     4861 ggagcattca aaatagttac ggttgcattt gttgtacaac ctgcctgatc agttacggtt
     4921 acattataat ttcctgttga aagtgcattg ttagaattag ttgtacccaa tcctgccgac
     4981 cacagataag aatatggagc agttccgcta ttgacagaaa tacttgcact gccattgcta
     5041 ccattatagc aagaaaccat ttgcttaatg taatttactg tggcaacagg tgatgttgat
     5101 gctgctacag caaccgttct tttagcagta catccattgg catcagtaat agtaactgta
     5161 taagttcctg aagctaaatt ttgtgacaat gcacctgtgc ctgaaggtgt ccattgataa
     5221 cttaatgcac cggttccgcc tgaagcatta acggtaattg ctccattatt tagtccacat
     5281 gaagttgatg taaccaacgg gcttgacatt accgaagtaa gtggttcaat aatagtaaga
     5341 ttggaaagcg tagtctgaca atttccgtca gtaacagtta cggtataact gcctgcaggt
     5401 atattcagca aatcctttgt tgttgcacca ttcgaccatt gataagaagc acttgcagaa
     5461 acaccggtaa cagacaatgt tatctcacca tcagtaccgt ttttacaact cacattttga
     5521 atggtgttta ctgatgcatt gatgttgtta ttgtcaggga cattaaaagt ctgagtaaaa
     5581 tcacaattgt tgttatctat aacagttaaa gtatagttgc ccggaagcag tgctgttgca
     5641 ttagcgcctg caccacccga tggtgaccat tgatattgat aaggtgcagc acctccggct
     5701 attgttgttg caatggaacc attggcagcg ccacatacgg ctgaaacaat attttgattt
     5761 tgtaaaacca aatcaggtgg ttccgttacc gttactgtgg ctaatgcact acatgcacca
     5821 ttagaaggag atactattac agtgtaggtt ccagcagtta cgtttgtagc agtattactg
     5881 ctgcttacat tgggcgacca ttgataagta aaagggccca taccacctgg cgtcagtata
     5941 atgcttccgt cattatcgcc aaaacaagaa acatcagtat gtgctgcaga aactgttgca
     6001 ccattaatgt tgattgattt attggttgga acagaaatca ggttgccaca caaatcctga
     6061 accggactta ctactgcaaa gttgtaaata cctgcaactg aaatagcagg ggtgaaagtc
     6121 accgtgtaaa ccttactgta atctgcacca ctggtacatc ctgcagaatt cactgctgta
     6181 atcatgtagg gtcctccggg gcctgttaat acaaaatctg ttgcctgtac agagttgcat
     6241 gttacgtttt cattaaatgt catcgttaaa gaattcgaac cgcaggtaag gggagttgtc
     6301 gttaacaaaa agggtggaaa aacatcaaaa atacttcctg tagaagcagt aaaatcaatg
     6361 gtatagccag attgtgtgga tgaaaagtta cttacattaa ttacataagt ctgacctggt
     6421 gtaacaggaa ttattggatt gttttgtcca ccacatggcc ctgctgtttg attattgggt
     6481 cctgtagttc cattgcaacc aagattaggt gcataattgc aactgacttc tattgatggg
     6541 ttggtgaaaa tatcggcaca agttgcagta gtaaggttat aaactgccca gtcataatcg
     6601 tcagcaggat tgtttggtgt aatggtaaaa ttaagatttc cgaaaaatgg tgttgtaaat
     6661 gtgtaccaaa catcattgcg ctcaccactt aaaagacagg aacttccgtt attaatttca
     6721 ttcggaatat ttcctgttcc actgtatgaa atgactgtgg agtaagtagt gttacagatt
     6781 ggaattgcat tcggacaatc ctgatttgta ggagattgtg cctgtgcaac aaaaacggtt
     6841 acagcaaaaa tgaatactgt attaattaat ttggaaaagg ttttcacacc acaaatatac
     6901 aatttcactt aataatcaaa cacattcacc aaaaaataca ccgttaaatt ttttaacatt
     6961 tagcgtcatg caacattttt ttaaataatt tcgccctctg aaaaatttta atttagtata
     7021 aaatgaaagt aacagttgta ggtgcaggaa atgtaggagc tacctgtgca aatgtgattg
     7081 ctcaccgcga attggttaac gaagttgtat tgctcgatat taaagaaggt actgctgaag
     7141 gaaaatcatt ggatatgtgg caaaccgctc caatcaatct ttatgataca agaataaaag
     7201 gttctacaaa cgattatgct gcaacagcag gctctgatgt tgttgtaata acatcaggtc
     7261 ttccacgcaa gccgggcatg agtcgtgacg atttaatcgc taccaatgca ggtatcgttc
     7321 gttctgtgac tgaaaatatt gttaaacatt ctccaaatac catcatcatt gtagtttcca
     7381 atcctttgga tgtaatgacc tattgtgcat tcttgacatc aggatttgac tctaagagag
     7441 ttttcggtat ggcaggaatt ttagatactg cacgttacag agcattttta gctgaggcat
     7501 taaattgttc tccaaaagat attcatgctg tattgatggg tggtcatggt gatactatgg
     7561 tgcctttgcc aagatataca actgtttcag gtattcctgt aacagaaatg atagctgctg
     7621 ataaattaga aagtattata catagaacta aagttggtgg cggtgagttg gtgaacctta
     7681 tgggtacatc tgcttggtat gcaccgggtg ctgctgctgc tcaaatggta gaagcaattg
     7741 ttcgcgatca aaaacgaatt ttcccatgtt gtgcatggct gcaaggtgag tacggacaga
     7801 aagatattta tcttggtgtt ccagtaaaat tgggtaaaaa aggtattgaa cagattattg
     7861 aacttaaact caatactgaa gaaaagcaat tacttgatgg cagtgcaaaa gctgtaaaag
     7921 aagtgatgaa tgtacttgat ggtatgcaac aaccggtaaa gtaaatttct ttctgtaaaa
     7981 taataaaatg ccgcttgttg tcaggcggca tttgtttttt aataattact taatctgctg
     8041 aactgatttt tttgctacat tcgcagaagt acagttctaa ccattgtaat attaaatttt
     8101 taacccgtta aatgtcaatg aacattatac ttgctgatag caaccgtaac gatttacttc
     8161 catttacttt tgtcagacct gtagcagaaa ttcgttgtgg tattttaacc attacagaaa
     8221 aatggaatcg cctgagtgga gcacaatgct catggctgac tgaaaaatat ctgtctgcaa
     8281 agtattcgtt agctaaagaa aaagttaact gtgttgccaa tgcatctgta cttattgaca
     8341 aagcgctttt caatgaaatc aaaactctga aaacaggtca ggcacttgtt aagggaaata
     8401 ctgtcattgc tgtttgtaca gaacaacaac aattcgaaac attggaagat gtaagctaca
     8461 atgaagcatt tgaaacagga cttgaagtga atgtgctgaa atatccttgg gatatttttc
     8521 gattcaatga taaagctatc aacagcgatt ttcaattact tacagaagga agaaaaagcg
     8581 caggatatga ttcttcaaat accgtaatta atgagcagca ggtttttatt gaagatggtg
     8641 ccaaggtcat gtgcagtatc atcaacgctt caacaggccc tgtttatatt ggtcgtgatg
     8701 ctgaaataat ggaagggtgc atgataagag gcccttttgc acttggtgaa catgctacac
     8761 ttaaaatggg tgctaaaatt tatggtgcca ctacaattgg gccacactgt aaagtgggag
     8821 gcgaagtaaa caattctgtt atttttggtt attccaataa ggcacatgat ggttttcttg
     8881 gtaatgcagt aattgctgag tggtgtaatc ttggtgctga taccaataac tcaaacctga
     8941 aaaataatta tggtatggta aaagcatata attattcaaa gaaggattat atcaatacag
     9001 gtttacaatt ctgcggactt atgatgggcg atcatagcaa ggcggcaatc aataccatgt
     9061 ttaatacagg cacagtaaca ggaatttgtg ctaatatttt tggaagtggg tttcctccta
     9121 aatatattcc ctcttttgcc tggggaggat ttgatgatga atcggtattc caacttgaca
     9181 aagcattgga gttagctgcc gaggtatata aaagaagaga attgattttt gacaaaaaag
     9241 aacaagatat aattaatcat gtcaacatgc ttgagctaat tctgccaaaa aattaaatgg
     9301 cacaagcatt gacagattac tattttaaac taataacagc ctgttggctg acaacttgac
     9361 gtaaactatg ttcaatccgg aagatataaa acacacactc attggcggca taaatgacga
     9421 tgagcccgag ttaataccat tactttcttc agaagatgaa gaacaactca ataaggaaag
     9481 catacctgac gaattgtcaa ttttaccatt gcggaataca gtactttttc caggtgtggt
     9541 tattcccatt acggtagggc gcgataagtc aattaagctg ataaaagatg cttataaaag
     9601 caataaaatt attggtgtgg ttgcacagaa ggacacaaac attgaagatc ccggattcaa
     9661 tgacctgaat aaaataggaa ctgttgccag tgtcataaag atgttgcgat tacctgatgg
     9721 taacactaca attatcattc agggaaaaag aagattttct ttacttgaaa tgacacagag
     9781 cgagccttat ctgagagcta aagttgaacc actgaacgat attgttgctg cattgaaaga
     9841 aaaagagttt aaagcaacag ttgattcgct caaagacatt tccttacaaa tcattcaaca
     9901 ttctccacaa attccaagtg aggccggatt tgcagttcgc aatattgaga gccctgcttt
     9961 tttaataaat tttatttcga gcaacatgca attggaagtt tccaagaagc aagacttgct
    10021 cgaaatgaca gatataaaac aacgtgcttt aaaagtactt gagtatctca cacaagagtt
    10081 gcagatgctt gagctaaaat cgcagattca gaataaagtg aaagttgatt tggataagca
    10141 gcaacgcgaa tattttttga atcaacaatt aaaaactatt caggaagaat tgggcggcaa
    10201 cacaccggat caggaattgc agcagttaaa gtctcgtgcc aaggataaaa aatggaataa
    10261 gcatgtagca gaaattttca ataaagaaat tgaaagattg ggacgcatta acccggcagc
    10321 agcagaatac agtgtgactt taaattatct cgatttatta ctcgaattgc cctggggcga
    10381 attcacagaa gataatttca atctaaaaca tgcacaaaaa gtacttgaca aagaccatta
    10441 cggattagag aaagtaaaaa cgcgcattct cgaatatctt gcagtgttga agctgaaaaa
    10501 agacatgaag tcgccaataa tgtgtcttta tggtcctccg ggagttggaa aaacctctct
    10561 tggtaaatct gtagccaagg ctttgggcag aaagtatgta cgcgtttctc tcggtggatt
    10621 gcgtgatgaa gcagaaataa gaggacatcg taagacctat atcggtgcaa tgcccggcag
    10681 aattattcag aatatcaaaa aggcaaaatc atcaaaccct gtttttgttc ttgatgaaat
    10741 tgataaactc accaacgatt ttcatggtga cccaagttct gcattactgg aagttttaga
    10801 tcctgaacag aacaatgcat tttatgataa ttacattgag cttgattatg acttatcgaa
    10861 tgtgttgttt attgctactg ccaacagttt gagctccatt cagcctgcac tgcgcgacag
    10921 gatggagata attgaaatca acggctacac agttgaagaa aaaattcaga ttgcagtaaa
    10981 gcacctcatc ccaaaacagt tggaagcaca tggattaaag aaacaccatg ctactttcaa
    11041 taacagtgta attgaataca taattgaatt ttataccagc gaaagcggtg ttcgtagtct
    11101 tgaacgcaat ctggcatcag tcatcagaag tattgcaaag agagttgcct taaatgaaaa
    11161 gtatgataag aagatagata aagatactgt agagaaagca cttggtgccc ctatattctt
    11221 gcgcgacaga ttttcagata acagtgttgc cggagttgtc acaggtttgg catggacatc
    11281 agtaggcggt gatattttat ttattgaaac cagtttaagc agaggcaaag gacgcttatt
    11341 acttacagga aatttgggag aggtcatgaa agaatctgca cagattgctt tagcttattt
    11401 gaaggcacat gcagcagatt ttggcattga ttatcgcgtg tttgatagat gggatattca
    11461 cattcacgtt cctgaaggag caactcctaa agatggaccc tctgcaggtg tcacaatgct
    11521 cacttctctt gcatcagcat ttacccaacg taaagttaag ttgaaattag ctatgaccgg
    11581 tgaaattact ttgcgcggaa aagtactgcc tgttggtgga attaaagaaa aaattcttgc
    11641 agcaaagcgc gcaggaataa cagacataat tctttgtgcc gacaatgaga aagatattaa
    11701 agacatcaag agcgaatatc ttaaaggact gaaatttcat tacgtaagta aaatgggtga
    11761 ggtaattgac attgcattgc ttcgcgaaaa agtgaaaaac ccattgaact ttactatcag
    11821 tcagctcgaa agaaaagaaa ccgtttaaga tttatttttc tactacagtg ggcttgaatt
    11881 ttgctttttc aatggcatta ttaagttcaa atccaataag caactgtatg cagttaaagt
    11941 tcagataaat tagaattact atcagcgttc ctatagaacc ataaatttta ttgtactgtc
    12001 cgaaattcag aataaaaaag ttgaatgcaa tggatgtaac agctataagg atagttgcca
    12061 gaatacttcc gggtgaaaag aaatgccatt ttttactgcc accggaagga ccaaaaaagt
    12121 agagtgatga tatggctgtg aaacaaagca aaatcagaat aagatatttg cctgcgagca
    12181 acaaataaac tgagctctga tctaagttta gaatgtgatt attgaaataa tgcagaataa
    12241 cttctgtgat gataattagt aatattgcta tgattaataa tgtcgctaac agaacagtta
    12301 gatataatgc aactaatctt tgtttccatc ctttacgttt ttcgtgcatg atggttttgt
    12361 tgaaaccttc tatcaatgca aacacaccat tggtagaaac tattaatgcc agaataaaac
    12421 caaatgaaag caggcctccg ttttgtctga caataatttc ttctattgtc ttatatgcag
    12481 tgtcataagt gttgtaggga agcatagatt gtaacagact taacagacgt tcctgaaagc
    12541 ctgcaatcgg tacgtaagga atcagtgtaa aaagaaaaat gattgcagga aagatggcta
    12601 aaaaaaatga aaaggcaatt gctctggctc ttatttgtaa atcgccttta aataattcac
    12661 gaagaaaaaa ttcacccacg tcataaattg gaattttttg aaagccgggt agcgtaattc
    12721 tacgtgtgta atttaacagt agtgataagt attcgagcaa cctgcgtttc attggcttgc
    12781 ctttaaactc atgtccatac ttttaaaaga atgtgttaat gcgccaacag aaatatagtc
    12841 aactcctgtt tcagcataat cttttacgtt ctgaagtata atgccaccgg aagcttctgt
    12901 ttcaaaacga cctgaaatca tggacacggc ttctttgatt aatgcaggtt gaaagttgtc
    12961 gagcattatt ctttcaacgc aacctgtgtt tagcacctgt tcaacttcgt ttaagttgcg
    13021 tgtttctatt tcaataggaa tattcaggtt tctgtttttt ctgaaagtat ttgctgattc
    13081 aatagcttgt tttattccac ctgcataatc aacatgatta tctttaatca taatcatgtc
    13141 atacaatcca aagcgatgat tgtgcccacc gccaatacga acagcccatt tttctatgta
    13201 acgattcagt ggtgttgttt ttctggtatc taaaattttt gcttttgtat gtttcacctg
    13261 ttctgcatat aggtatgtag tggaggcaat gccgctcata cgctgcatac agttaagtac
    13321 caaacgttct gtcttcagta tagaaagaac gcttccttca actgtaaaag caatttcact
    13381 tttatcaacg tgttgcccat cttttttaaa gatgttgatt tgcaacgaag cgtcaacagt
    13441 tttaaatatt ttttctgcaa gttcaatgcc tgcaagtatt cctttatctt taataataag
    13501 attggccttg ctttgagcat ctgccggaat gcaacttaaa gcactgtaat caccgggtcc
    13561 gacatcttcc tgtagtgata atgaaatgta ttgatctaaa tccatgataa ttatttctgc
    13621 tgcaaaattc agctttttga ttcaaagaat catgtaggat acgatttttt taaaatacta
    13681 aaaaggaaaa agccgtaaac aagagtgaat acggctttca aaaactatgg ggaagatatt
    13741 cttattcttt ttcgaagcgc aattcctgaa tagaagaatt gccaccactt tcttttacaa
    13801 agaaataagt tctgatattc tgaccttgcg ttgtagtgag tgttcctatt gcataacggg
    13861 caccttcctt cgaaacgcct ttatggatta tggtgaaact ttttggtgcg tttttactga
    13921 aaaaatcttt aagcaccatt tctgcctgcg ctttactgta aacttcttcc tgattaaata
    13981 ttgtcaaatc aacattgtta ttaaagaaac gagcaatttg tctggcatcg ccagaacgga
    14041 tggcagtgcc gatatcatcg aaaacatcag ctgcctttac aacggtaact atcagcatga
    14101 cagaaagaag aagcttgatg gtttttttca tccttgataa attatgtttg tgaactgaca
    14161 aaaatcaagc caagagcgca taggaatgaa aaacttatga atgcatttta gcataaatct
    14221 tacagtaata attaaacact taaatgtaaa tccgaaacaa tattaaggca taaaaattta
    14281 ctttcaaaac ttaatttcag atttttgtaa ataatcatta ttggataacg taattatgca
    14341 gatcaaattg ttaatgatag gtaagaataa ggatagtttt gccgatgccg caatagctga
    14401 gtatgcgagt agggttgaga ggtttaacag ttttgaagtc aagtatctga aagaagccaa
    14461 aaacaacaaa aaccttacag cagcaatgga tgctgaggct gaaaatattt tcaaggagtt
    14521 atcgccaaat gactttgtta ttctgctgga cgaaaaaggc aaagaataca catcggtgca
    14581 gtttgcaaca aaaatcgagc agttaaggat taaaaatttc aaacaattgg tttttattgc
    14641 tggtagtgct tatggttttt cggaaaaagt ttatgcacga agtaatgaac tgtttgccct
    14701 gtcaaaaatg acattcaccc atactcatgc aagattgatt tttgccgaac agatttacag
    14761 agcatttact atcctgaata atcatcctta tcatcatatt taatttcatc ggcatactat
    14821 ttggtaagat ggaaaatcta attttgcact ttcaaaaatt tttgatcaga aatctatatg
    14881 tgcggtatag ccggcattct caattttcag cataaacctg ttgatttatc agtattaaag
    14941 caaatgtcag acaccctttc tcacaggggg cctgatggtg aaggtatttg gcataacgaa
    15001 cagaaaaata taggacttgc acacagacga ttggcgatta ttgacctttc tgatgaagcc
    15061 aggcagccaa tgcattattt aggtcgttat acaatagtct ataatggaga aatttataat
    15121 taccttgaac tgaaggctga ccttgaaaag aaaggctatc agtttgtgtc gcactccgac
    15181 actgaggtga ttcttgcagc atttaaccat aagcgggaaa agtgccttga agattttgac
    15241 ggtatgtttg cctttgcaat atgggacagt aaattgcagc agttattttt agcccgtgac
    15301 cgttttggag aaaaaccttt acgttactat atggatagtg aacggatagt ttttgcttcg
    15361 gaagtaaaag cattgtttaa agccgaagtt ccgaagaagg tttctaaaga gaagatggat
    15421 aactaccttc agaataatat ttcaatttat aacactgaaa cattttttga tggaataaag
    15481 aaactgccac atggtcatta tgcttttgtg cagaatggca atattgatat ccgtgagtat
    15541 tacagattgc aatacaagaa tcttgtatcc atggatgacc atgaatatgc agaggagttc
    15601 aaaaggttgt tttttttatc aataaacaga cgattgcgaa gtgacgttcc tgtggcaaca
    15661 tcattttccg gagggcttga ttcctccggt attgtttgtg taatgcatca actcaaggaa
    15721 aaagttaatt ataaattatt ttcagcacga tttggagatg atgtaaaaga tgaaggtaaa
    15781 tggatgaagc atataacgga tttacttcac tttccacaga taaataaaca gattgagccc
    15841 caagaaattt tagaagatat tgaaaaaatt gtctatcata atgagattcc cattggatcc
    15901 acatcagtaa cagcacaata tctgctttat aaggaagtta aaaatcatca tgttaaagtt
    15961 attcttgatg gacaaggtgc cgatgaaatg ctggcagggt atgggcatta ccgtttccat
    16021 tatctaaacg atttgttgct tcgcttcagg gtagcggaat atattaatga aaggcgagca
    16081 tataatgatg catatcaaac ttcactccca attggccctt ggcaactgac aaagatgttt
    16141 ctcacaaaaa ctgtaaagcc atttgaagat aacactcggg gatatcagtc atttaaagct
    16201 acactttata atgatatgat gatgagtctt caattgttgt taaacttctc tgatagtaat
    16261 tctatggctc acggtgtgga agtaaggctt ccatttttgt atcataagtt gattgacttt
    16321 gccttttcac tacctgccaa tcagatttac agaaatgcaa caacaaagta tgtttaccgc
    16381 aatgctatga atggaattat tcctgatgct attctcaatc gtaaagataa ggtagggttt
    16441 gcaccacctc aggaacgatg gcttcctcaa tttaaactcc cggaaaatgc aagacttcgt
    16501 gacatgggtt taacttattc tgataaccac tggcgcaatt acatctcaga agtattttta
    16561 aaagtagccg acagccgata ctgattaatt ggtttttcgc aataaatgtg ctgtatttgc
    16621 ttgcgcagtt ggtgtaacta caacatcatt tatacaaaca tgtgcaggac gggttgcaac
    16681 aaaatataca atctctgcaa tatcttccgc cactaatggt tcaaatcctt tataaacagc
    16741 attggctttt tcaacatcac ctttcaatct aaccaaagaa aattctgttt ctgcagcacc
    16801 tggatttact gcactgactt ttatgccatg ttctaataaa tcaatacgca tactttttgt
    16861 caaagcatct actgcatact ttgtggcgca ataaacattg cctttcatat aagcatcttt
    16921 tccggcaata gaacctatgt tgatgatatg tccgcttttt tgttttatca tcagtggaat
    16981 gatattttta gtgacataaa gcagtccttt cacattagta tcaatcattg tgttccagtc
    17041 ttcagtcttg ccatcctgaa tcagatttac tcctgctgca agtcctgcat tattaactaa
    17101 tgcatcaatt tgtttccatt tgatggggag attttcaata gcttcattaa cctgttgttc
    17161 gtttcgaaca tcaaaaggaa gactgatgac atctgctcca agattcttta tttcatcttc
    17221 taaagtgcga agtctgtcag ttcttctacc attaataata atgctccaat ggtgttgtgc
    17281 aaattttaat gctatggctc ttccaaatcc tgctgttgca ccggtaataa aaattgaagg
    17341 cataataatt tattttttta taatatttaa cttactgttt ttatagctgt aaccgaaata
    17401 aataatcagc cctacagcca gccaaatcga aaacccaatc cagtttgata tgcccatttc
    17461 actcatcatg tataggcagc aaaccaaacc taataatgga atgagcgata gattgtgttt
    17521 ccaggttaaa acacaaagat acagacatga aatgataaat atccatgtag gtattttatg
    17581 tttaaaaagt ccaagacccg attcataaat ttctttatgc gacaatccgt agtccgttag
    17641 tttctggaga taattatcgg tagttatttg tgaaaaataa tttgcaaggt cgttgtcggc
    17701 tttagcaaat gctgctgagt ctttcaaaga aacagtgttt accagatctt taattctgtc
    17761 agctcctaaa gaaggaatca gattttcggc agagtagggt tgtggagtgt tcttcaaaaa
    17821 attggaaacg ccatctttat tatatgacca cgacaatgct gcagttaaaa tgagcattaa
    17881 cgggaaaata tatttagcat ttacataagg tgtattaaaa ctacctcggg gcgcattagg
    17941 cgtgatttgc attttcaaaa ctccggcaca aactaacaca aaggcaaata aggttccaat
    18001 gctacaaagg tctgtaacca ttgtaaggtt catgaaaagt gctggcactg caactacaaa
    18061 gccggttaca attgatgcaa acgatggtgt tttatatttt gggtgaatgg ttgcaaattt
    18121 cttaggtagc aaaccatctc tgctcatact catccagatg cgtggttgac ccaattgaaa
    18181 aaccaacaat acacttgcca ttgcaacaat agcactcact gcaataattg ccgacatcca
    18241 ttttaaattc aatacactga aaacataagc caatgggtca ccaacagcca aagtgctgta
    18301 gtttaccatt cctgtaagca ctaatgctat ggcaatataa agtattgtac agattattat
    18361 tgcccacatc attcctcgag gtaaatcacg ttgtggattt ttacattctt ctgctgttgt
    18421 gctgatagca tcaaaaccta tgtatgcaaa aaatactgct gacacacctt ttaaaacacc
    18481 gggaatgcca ttgggtaaaa aaggatacca gttatcggta ttaatataaa acaaacccac
    18541 agcaatgact aaaagcacaa cagaaagttt tatgattacc atggcattac ctgcattttt
    18601 cgactcctta attcctctgt aaacaatcca tgtaatgatg agaataatta aaagagcagg
    18661 caaatcagca acaatatgca atgggcctat tgttggagca ccctgccatg ctaaataggc
    18721 atcacgcaaa ccggcatcaa gactttcaag tgttcttccg ctaagcatca gtgcgcctgc
    18781 tttgctgaac ccttttgctg ctgtaagata atccatcgtc atccacagtg gcaaatgtat
    18841 gcccatactg tcgagtaacc ctgttaaata atcgctccat gaaatagcaa cggtaatgtt
    18901 tccaatgcca tactccataa tcaatgccca tccgattatc catgcaataa attcaccaaa
    18961 ggctacatag ctgtaagtat atgcactccc actaacagga actaatgatg caaactctgc
    19021 ataagcaaaa gcggtgaaac cacaggcaat ggcagtgaaa agaaacaata aaatcacacc
    19081 gggtccacct tgtgcacttg cattaccaat agtggaaaaa attcctgcac caattatggc
    19141 agcaatgccc aaagcagtga ggtcgcgcac acctaaatgc tttgctaacg agccatgctg
    19201 tgaggcatca tcaccggcac gtttcataat ttcatcaact gttttctttc tgaataaatt
    19261 catgaacaga cattattatc cggtcaaaaa taaaataatt tcatccgcaa aggtcttttt
    19321 cttgtttaat gttgattaat tcattatata ttctcatgtc aattggctgc tattaaatag
    19381 ttttgcagtc atcaataatt caatcattta tccgttaaca caaaggtaaa atcacttatt
    19441 tatgaaaaag ttttttcgca tttctataat tgttattgca gttctttttg cagcagctct
    19501 tgttgcacct tttttgttta aagataaaat tattacagcc attaaaaatg cagctaacga
    19561 aaacctgaat gcaacattcg attttaaaga cctggattta agctttatcc gtagtttccc
    19621 ctctgtgtat ttagcattaa acgatgtttc cattgttggg aaagatgcat tcaaagacga
    19681 tacacttatt tcagcttcgt ccatacaggt taaagctgag ttttggaaac tcattacttc
    19741 aggaaacaca caggttaaat attttggatt attgcagcca cgcattcatc ttattgctct
    19801 taaagacgga aaggtaaact gggacatcac caagcctgaa aaggaatcac caacaaagtc
    19861 ggaagagtct tcaccatttc atttttcatt acagaaatat gaaatcacga acggttatct
    19921 tacctacaac gatgaggctt tggcaatgaa taccaaaata gataatctga atcatacagg
    19981 caagggtgac tttacacagg ataatttttt actgaatacc ttaacttcaa ttgataaact
    20041 tacttatact tatgcaggtg taagttattt gtatcaagct aaagttaacc tcaaagcaga
    20101 tttaaacatt gagtctaaga catcgaaata cacttttgct gacaatgagt taagcataaa
    20161 cgaatttcca ataaattttt cgggctttgt ggctatgccc acagacgata ttagtatgga
    20221 tttgaaattt aattcaccac aaacagattt taaatatctg atgtcattgg ttccgggtgc
    20281 atataaagaa aattttaaag atgtaaagtc ttctggaaag ctggccttca atggttttgt
    20341 aaaaggtgtt tattcagaac ataccatgcc cggatttggt attaatttaa ctgttgacaa
    20401 tggaaacttt aaatatcctt cattacctgc atcaatcaac aatgtaaatc ttaaattagc
    20461 tgtttccaat cccgatggcg aacctgacca tacaattatc aacctttcac aggcacatgc
    20521 agagttaggt gccgaaccaa ttgatgcacg attgtacgta actactccgg tgagcaatgc
    20581 cacatttgat gccatgctga aaggaaccat aaatttagcc aacattaaaa attatattcc
    20641 tatggatgag ccatcattat cagggatttt caagagcgac ctaagcatca gtgggaatat
    20701 gaatgctgtg gagcaaaaac aatttgaaaa aattaaggcc gcaggatgga tgtcgcttac
    20761 tgctatgaac tacaaaccca aagatggtca cattacaaag attaatgaaa tgaaactcag
    20821 ttttaatcca cgcactgttc agttagagaa tttggatgca gttgtaggaa aaagcgattt
    20881 cagagctaaa ggtgtaatgg ataatttgtt tggctattat tttaagaaag agttgctgaa
    20941 aggagaattt actttaaaca gctatttggt tgacctcaat cagtttatgt ctgatgatgg
    21001 caatgctgca gcaacagaca ccaccgcatc tggcgttgcc gaagttcctg agaatgttga
    21061 tttcacattg acaacttcaa tcgggaaact tctttacgaa aacaaagtca ttgaacaatt
    21121 gaagggtaat gttgccatgc gcgaccgcac attgggactt aatgatgtta attttaaaat
    21181 gtcaggtggc acagttgtca tcaatggtgt ttatcagacc aaggagcgca actcgcccta
    21241 ttttaatttt gatctggcat tgcagaactt tgatattcag aatacagtta aaactttcag
    21301 tacagtaaag aaagtagctg ctattgcaga aagatgcaat ggaaaattct cttctgcatt
    21361 taatgtcagc ggaaagatgg atgagaaaat ggatcctgtt ttatctacac ttaatggtgg
    21421 aggaaagcta actacacaaa ctgtttctct gaacaatttt gaacccataa ataaattagc
    21481 agatgcgttg aagatgtcgc aatacaaaca gatgaatctt tcaaacgtga acctgtcgtt
    21541 taaatttaaa aacggaagtg tttttgttga accttttgaa acttcacttg caggcagcaa
    21601 ggctgtcatc agtgggcaat cgggttttga ccaaagcatt aactataata ttgacctgag
    21661 cattcccaag gcacttatgg gaagtgcagc tacaggtgtg gtgaatggca tgtttgctag
    21721 tatcaacaaa gctacaggtg catcgttcag tatgcccgac cctgtaaaag ttaaagtaaa
    21781 tatgggtggc acggttcaaa aacctgaaat taaaacatcg cttgccgatg caggtaaagg
    21841 tttggttgaa ggggttaaag ataaagcaat ggagcagttg gatgctaaaa agcaggaact
    21901 ggaagacaag gcaaaagcag aggccgaccg tttgaagaag gaggcagaag agaaagtgaa
    21961 gtccgaagca gacagactaa aaaaagaagc tgaagcaaaa gctaaggcag aagcagaccg
    22021 tttgaagaaa gaagcagagg agcgagctaa aaaagaagcc aaagacaaac tgaataatat
    22081 cttcggtaag taaatttctg tagtacttaa atcatttttc agagggtaaa agtgttatct
    22141 ttgcaccctc aaaaatttta ctatgggatt gaaatgtggt attgttgggc tgccgaatgt
    22201 aggtaaatca acactgttta attgtttaag taatgccaaa gcacaggctg caaattttcc
    22261 attctgcacc atagagccaa acattggtgt cattacagtt cctgacgaca gactgaataa
    22321 gttggaaact ttggtaaagc cacaacgtgt tgttcctact actgtcgaaa ttgttgacat
    22381 tgcaggattg gtgaaaggtg caagcaaggg agagggttta ggtaatcagt ttcttggaaa
    22441 catccgcgag acagatgcta tcattcatgt tttacgttgt tttgatgatc ccaatgtggt
    22501 gcacgttgac ggctctgtga atcctgtgcg cgataaagaa accattgact ttgaactgca
    22561 gataaaagac cttgaagcta ttgataaaaa aattcaacgc attgaaaaaa ttgcacgtgt
    22621 gggcaatgat aaagatgcaa agaaggccta tgatgtgttg caggttttta aaaaacattt
    22681 agaaagcggc agtgcagcac gcagtgcacc tgtgcagtcc gaagattttc aatatgtggc
    22741 tgatatattt ttactcacag caaagcctgt actgtatgtt tgtaatgtgg acgaaaaatc
    22801 tgtcctaacc ggaaacaaac atgtagatgc cgtgcgcgaa aaggtaaaag atgaaaaagc
    22861 cgaagtgctt gttattgcag cagctattga agcagagatt acacaattag aaacttttga
    22921 agagcgtcag atgtttttgt ctgatttagg acttacagag tcaggtgttg ctaaattaat
    22981 taaagcagca tatcgcttgc tgcaactaca aacgtatttt acggcaggtg aaaaagaagt
    23041 tcgtgcatgg accatcacac agggtatgac agcgccacag gctgccggag ttattcacac
    23101 cgattttgaa aaaggattta ttcgtgcaga ggttatccgt tataatgact ttgtccactt
    23161 tggctcggaa caggcttgca aagaaaatgg aaaactgtct gtagaaggaa aagagtatgt
    23221 ggtgaatgat ggcgatgtaa tgcacttccg ctttaacgtc tgattatttt tttcttttcc
    23281 agggc
//
LOCUS       DAOOOI010000032        22756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_422_length_22756_cov_10.633893, whole genome shotgun sequence.
ACCESSION   DAOOOI010000032 DAOOOI010000000
VERSION     DAOOOI010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 22756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22756
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_422_length_22756_cov_10.633893"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="PKX59_05765"
     CDS             <1..1007
                     /locus_tag="PKX59_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOZ90409.1"
                     /translation="KDKDNTNASPETEYGFISGRVHTPNNTNVGSALIIAGEYNTKSN
                     AQGEFILRVPTGFYKLKIQTGSGHVFKSMVGVEVKANDTLRLSDSESLLQQIKQLAFI
                     PGAYDRIETIIIDSLGYAATAISVNDLDNLSYMQSFGGIFFDCGKSGSLDSLKYTNIS
                     NYLVNGGSIYASDWAIEYLTGDGSWRPALGSNTLNVSASKNQSHEAGYSTMATCLSSE
                     VGGFIPDSALCASKSGAIGLITNALINDPDIVTLLGKDSMDIEYNLGSWEKISHVDIP
                     FTTMIEKNNPGPGPLAVSCDLNGTAFGGRIIYTTFHNHPQGNISTDVKLVLQHFILSL
                     "
     gene            1146..2369
                     /gene="lhgO"
                     /locus_tag="PKX59_05770"
     CDS             1146..2369
                     /gene="lhgO"
                     /locus_tag="PKX59_05770"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HOZ90410.1"
                     /translation="MSSQAEYDIVVIGGGCVGMASAYKLNLRFPHLKIAVLEKEDRLA
                     AHQTGRNSGVIHSGLYYKPGSYKAKNCVEGRRELVAFCKEHQVAHDVCGKIIVATDEK
                     ELPHMNRVFNNGLQNGVEGIEKINAQQIKDIEPFCTGIEGIWVPCTGIVDYVGLTNKL
                     AELIEQKFPGSKVYTGHEVTAFEKEDGNTTVVTNKQKFKTKHLISCTGLFSDRMAKAD
                     GVNPNMQIVGFRGDYYDLTEKGMHKVRNLIYPVPNPEFPFLGVHFTRMIQHGVECGPN
                     AVFTFKREGYGKTDFDLKDTLQALAYIGTWKLFFKHWRFGLDEYKRAFSKKLFLERLQ
                     RLIPSLEMDDITPGRAGVRAMALDANGNMIDDFKIEYSGNAIHVLNAPSPAATASLAI
                     GTAVMEMAEKHFQIH"
     gene            2525..2899
                     /locus_tag="PKX59_05775"
     CDS             2525..2899
                     /locus_tag="PKX59_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOZ90411.1"
                     /translation="MRTTILRFTFLVSSILLFSFSSHAQESKTDTIKVYGNCNMCKEK
                     IEGSLKEKDGITAKNWDKKTKLLAVTYNPSKITKEQIGQKIADVGYDNQYATATEKVY
                     KKLHSCCQYDRPKQPAGDEKNK"
     gene            2921..3697
                     /locus_tag="PKX59_05780"
     CDS             2921..3697
                     /locus_tag="PKX59_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90412.1"
                     /translation="MKKQLLTLILFAITSLGMAQTATDFTANDCNGNSHNLFTELNSG
                     KVIVLCWVMPCASCTGPSLTTYNVVQSFQASNPNKVFMYTCDDFANTNCTSLNSWANG
                     IGVMQQSWSLRFSNAAINMTHYGSPGMPKIVVLGPNHAVFYNSNNTVNATNLQNAINN
                     ALATTGLNESNLPASSLSVLPNPAQNEAKIKLNLLNKADVILECYNLEGKKVQEIFSG
                     NLTKGEHTIPVNLEQFTDGMYLIKFSDGTQTQFINLIKSN"
     gene            3746..4888
                     /locus_tag="PKX59_05785"
     CDS             3746..4888
                     /locus_tag="PKX59_05785"
                     /inference="COORDINATES: protein motif:HMM:NF039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HOZ90413.1"
                     /translation="MHNKVQLKFSLALLLTCFSVSDTFSQCCAGGSGSPIAGGTSQGV
                     LQEKQVEISTNFQFISTDKFYKKNKVAPDSVRTFDGFNSTYQYFRLAYGITKSLTFSV
                     ESGYYYQKKETGLHKDAATTYNAKGFGDLILFPRYDIINWTEEKHRTEVTVGLGFKIP
                     LGSYNDSTGNVEPFSGYTYYVTNPTSVQLSSGAHDIIFYTFLYRGFTEKNFRVFANAL
                     YIKKGYNPNGEKLGDFASVGIFAGKTFFNNLGVTLQARYEWMDRMKVNESISLFGKPS
                     NYFPKATGYKKIFISPQLSYTKGKFTVYGSYDIPVYQYLNTSEHYTQMGSQHQATLGV
                     TFRFMALNSYLKEKSGVTYVCPMHPNVISDKPGKCPDCGMDLVKKK"
     gene            4997..5626
                     /locus_tag="PKX59_05790"
     CDS             4997..5626
                     /locus_tag="PKX59_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOZ90414.1"
                     /translation="MLFVQPSYLIRLFYSGLLWRMPTRQPIVYLTFDDGPEPEATPFV
                     LSQLKKWNALATFFCIGNNIEKHQDLFSRVVSAGHQIGNHTQHHLNGWKNNLNTYLAD
                     VEQCSTHISSGLFRPPYGKITQKQIRSLRSQWQIVMWDVLSYDFETNVSPEKCIQNVM
                     QHVRPGSIIVFHDSKKAFKNLSETLPVILEKLSAEGYSFKAIPPYRPLQ"
     gene            complement(5598..6269)
                     /gene="rsmI"
                     /locus_tag="PKX59_05795"
     CDS             complement(5598..6269)
                     /gene="rsmI"
                     /locus_tag="PKX59_05795"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOZ90415.1"
                     /translation="MLYIVPTPVGNLEDITLRALNILKSCDIILVEDTRNASVLLNHF
                     GISKKVFTHHQHNEHAAVNELIRLLKDEKKTIALISDAGTPGISDPGFLLTRACIEHE
                     IELTCLPGATAFVPALVMSGIPCNSFVFEGFLPAKKGRQTRLKLLQEETRTMVFYESP
                     HKLIKSLHDFITNFGADRKVCVVREISKMFEEKKRGTLAEVCEYYTNHPPKGEIVIVV
                     AGKEE"
     gene            complement(6299..6997)
                     /locus_tag="PKX59_05800"
     CDS             complement(6299..6997)
                     /locus_tag="PKX59_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90416.1"
                     /translation="MKRVQIIVLFFSLLFCGTASAQIDSLGVIAISPQYPQCPDSAFM
                     QQPYFNIQVSIKNYNANSTFTGGLMLKLQSDTLPEDSIVLNAGTVYTIQPNGSINITI
                     QNPYFFNVASYKLGSNVVVVWPVSTQGGTVVKYNPYLACVLFLPFVGINEYDRDKLSV
                     MPNPSNKFIDLNIPSNIGLKKVRIFNPTGEEVRNYSVDLSNRITVEQLASGIYFLEVI
                     TTDDHVYNTKFIKN"
     gene            7097..8566
                     /locus_tag="PKX59_05805"
     CDS             7097..8566
                     /locus_tag="PKX59_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90417.1"
                     /translation="MDILNQLITGLNKEEVRFFKMYINRVEKTDNRKDVQLFDYIRKS
                     GDKFSDAIIFKKLYQPQEKNSYYRLKNRLIREVNKSLTLQHFDDDMVVYIFRLLALVK
                     FYLNKNALKPAYFFLRKAEQKANDTESLELLDIIYGEYIRLSREMTSINPEIYIHRRK
                     QNSEKLNQLRVIDDILAVVTYRLKITQNLSSKDDTVISLLQKTTDDFIGDPKIIKSPL
                     FRFRIYNAISQLLLQSHQYVLLEDYLLKTLKIFNQEKLFTKNNHETKLQLLVYLVNSL
                     YKNNKLKKSLEYINMLHQEIKAYNHLFYDKFLFYYYNALVINYSKTDKQKEIVTLQEM
                     EQNEKINANLFYRQYILLNFALAYFDQKEFSMSAKSFTRLQMIPDYKETDESLRMKVA
                     VAELMTRYELNDYNTFEYKLKQFNKDFRSGLNKPENEPEKIMSEILKQMMSVSNRKNR
                     VIELRIAQLKTLSKDEDKSELINYSDWLQEKKLLKHKIR"
     gene            complement(8559..10949)
                     /locus_tag="PKX59_05810"
     CDS             complement(8559..10949)
                     /locus_tag="PKX59_05810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ90418.1"
                     /translation="MQKRLYIVILMLLALAVAFFLAWFNYQNTTALSRTITLLQKPDT
                     RLSDLKELILNVNLAESHVRSFAITRNEKNLESYYKQVEQTDSQLVKLKNLFPANSQA
                     FDSLTASIHYKFDIYDKLIELRYRQLINTAMSKISDKVKDVDVYQNTDSVETSKPGFF
                     KKLFSSGKKIKELEEKAMLLDSLNNLQAQKLSTIKQTISEAKENEIKMVAQLSDGELL
                     LLEQDRMLKEKIQSQIALLEENIRHNNENRSLEVISQSQKQLKTVVIATITASVLILI
                     LSIIILSDISRANRYKIQLEEARARSEQLARFKEEFLATMSHEIRTPLSALTGFINRL
                     QQTNTTSEQRQYVNTINMAGQHLLNIVNDVLDLSRIETGKLNFSQEPFVIYKEVNDVV
                     TLLRQKADEKNIALILEASTIRNITVSGDALRLRQVLINVIGNAIKFTEKGTVFVKVK
                     KQADDFFNFEITDTGIGIAEEKIAKLFDAYQQADNIERNYGGTGLGLAITKKLVELQK
                     GTISMKSVPEKGTTVSIMLHFPLTKQTETNIAETEFVSLHGLRLLVAEDDVLNRQLIS
                     AMLKDSGAEVITAESGKQAIEKILLYNPDGVLMDIQMPELDGRNATSFIREHISAMLP
                     LIGITANMMNDAKNECIAAGMNAVILKPFKLHELSEILLPLIDKNREVQHKPLLSYSL
                     DSLFRTSNGKSDFVIRMLKVFISSNQSLIEKSIHFAKESDFQQSAAAVHRMIPGFRQL
                     DLDTFASILKNIEWLMLEGTEKNAATELLQQCAEQFEKLISAMQADIDRLEKEATT"
     gene            11176..11712
                     /locus_tag="PKX59_05815"
     CDS             11176..11712
                     /locus_tag="PKX59_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90419.1"
                     /translation="MKKLILIALPIVGMTLFGCENYKEQAAQLTRERDSLVSLNVAGT
                     QTIDEFITTLTDVENSLGKITEKQNSIAMTADRNPEAARNSRERLNAQIEEINRMMQE
                     NKDKVEQLSKKLKGSNASAAKFQKMVKSLQEQLTQKEQELALLNEKLAGLNIEVGYFK
                     NKLRFSYNNKSKSGYCNC"
     gene            11642..12043
                     /locus_tag="PKX59_05820"
     CDS             11642..12043
                     /locus_tag="PKX59_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90420.1"
                     /translation="MATLKTNFDSLTTTNQNQATVIADQTTKIHTAYYTVGNYKSLRD
                     KQVLSKKGGVLGLGKKATVVPAIKNDNFTKIDYTQISTIPVNSKKVELVTTHPDGSYK
                     IEKNNDLVSDILITDPEKFWSASKYLVVLTK"
     gene            12205..13239
                     /locus_tag="PKX59_05825"
     CDS             12205..13239
                     /locus_tag="PKX59_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HOZ90421.1"
                     /translation="MQKKFFFLILFAFGFIKLYAQTEPAKTTEKESLVNWISLSEAEK
                     HNKEVPKPIIIDFYTSWCGWCKVMMNTTYSDPAIASYINANFYPVKFDAETKDTIEYD
                     GVKYKPTGTEKRDPNEFAVKMLNGTMMYPSTIFMNKAANFTMSAQGYLETKKIEPMLV
                     FTLENGFRNSSYEDFNAQFQKAFYDSLQTKSYESVKWQTPLQFFIKDKKPDQKKKIIF
                     INTEWCNTCRVMYITTFIDTAVSSLLSKHFELVNFNPETNDKLYFQDKEFDNIHSKEL
                     PFHQLVYALSRNGLLFPQVIFMDEKNTVVDAIPFYLNPNVFKNIVRFYGEDIYKTKNW
                     ETFIKEQETK"
     gene            13297..14520
                     /locus_tag="PKX59_05830"
     CDS             13297..14520
                     /locus_tag="PKX59_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HOZ90422.1"
                     /translation="MKRLKNPVTFIFITVLVDCIGIGIIFPVIASLITEVSHVPVNKA
                     TTYSGWMMTLYALMQFIFSPVLGGISDRYGRRPVLLLSLLGLGIDYLFLSVAHTLTLL
                     FIGRIIAGICGASFTTSFAYIADVSPSEKRAQYFGMMGAAFGVGFIIGPVLGALASHW
                     GVRAPFVVAACLSLLNFLYGYFILPESLKPENRRAFHWKRANPLGAFIQIKKSKNIRT
                     LIFTMFLLYLAGQVMPSIWAFYTKFTYQWSDAEIGYSLAFVGVMVAIVQGGLIKWSQT
                     KLGSHTSIYTGLAFYLVGLTLFAFANQSWMLYAFTFIYALGGIGPPSIQGIISGLVPP
                     NEQGELQGMMTALMSVSTILSPLIMTNLFYNFTKPDTIYYFPGAPFAAAAILVLISFF
                     VVKRGINNLTRHQKI"
     gene            complement(14618..15577)
                     /locus_tag="PKX59_05835"
     CDS             complement(14618..15577)
                     /locus_tag="PKX59_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006518740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOZ90423.1"
                     /translation="MPVNFSEILVVGVSSRALFNLEKENEIFLKEGISGFRKYQLAHE
                     HEPLQPGTAFHLVQSLLHLNESAKRRIVEVIVMSRNSPETGVRVMNSVGKYKLDITRM
                     AFSGGEPLAPYIDAFDVDLFLSKDLKDVQNVIDSKKSAAAYIYEPPTEFKPTDNRVKI
                     AFDADAVLFSDESEHRYKTEGIEAFHKYEQEHQDVPLAEGPFAKLLIKLSKIQDEMPP
                     PIELSPLRLAIVTARNAPSHIRVIKTLRKWGVYVDEAYFLGGLSKDNVLKAFGAHIFF
                     DDQEVHLSESSKVVPSGKVPYTSDSPLLKLDVHPKANKAASKK"
     gene            complement(15646..16656)
                     /locus_tag="PKX59_05840"
     CDS             complement(15646..16656)
                     /locus_tag="PKX59_05840"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOZ90424.1"
                     /translation="MAALGKKCNFLNLIAVAVLLVVGSCKQPAQESTETKQETPATVN
                     IPVPAFNADSALHYLQTQVAFGPRVPGTDAHAQCADYLFQKLKSFGLQTQIQSETITT
                     FDSKKFRLKNIIAEYKPEAKQRVLLLAHWDTRPWADHDSVRKDQPFDGANDGASGVAV
                     LLEIARLLPASGLETGIDILLVDLEDYGQEEDDTRFPRQENTWCLGSQFWAKNMHRSD
                     YFAQYGILLDMVGGVNPVFPKEGTSLYYAPDVLSKVWNIAQNMGYGQFFINEPAPQTI
                     DDHLYINQLANIRTIDIVHYDNVKKEYPPYHHTHGDNLSIIDKNTLNMVGRVVMQTIF
                     SK"
     gene            complement(16631..18103)
                     /gene="cysS"
                     /locus_tag="PKX59_05845"
     CDS             complement(16631..18103)
                     /gene="cysS"
                     /locus_tag="PKX59_05845"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOZ90425.1"
                     /translation="MEFQPLNIYNTLSRQKERFEPLNPPFVGLYVCGPTVYSDVHLGN
                     CRTFISFDLIYRYLLHLGYKVRYVRNITDAGHLEGDRDEGDDKFAKKARLEQLEPMEI
                     VQRYTLGFHDVMRAFNTLPPSIEPTATGHIIEQIEMIKQIISNGYAYEVNGTVYFDVE
                     KYSKEFNYGQLTNRLLEDMIANTRELGGQDEKKGRLDFALWINAKPEHIMRWPSPWGV
                     GFPGWHIECSAMSTKYLGATFDIHGGGMDLAATHHTNEIAQSQACSNVQPCRFWMHTN
                     MLTVNGTRMSKSAGNGFLPGELFTGNHPLLEKGYSPMAVRFFMMQTHYRSTLDFSNEA
                     LQAAEKGYQRLMSAVKKLNSVKASDVTSVSLSSIRKNAYDAMNDDFNSPVMVAQLFEL
                     VRIINSANDGKEKMTKEDIAEAKSIIQHFVFDIAGLMDDTVSSGDQSVLDNVMQLVLE
                     MRNQAKAKKEFSVSDHIRNSLNAMGINVKDSKDGSTWEKM"
     gene            complement(18106..19065)
                     /locus_tag="PKX59_05850"
     CDS             complement(18106..19065)
                     /locus_tag="PKX59_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOZ90426.1"
                     /translation="MSQTERTLVLGSAGQIGTDLVEELRRIYGSDNVIATDIKDQPAD
                     FKAKGPFYMADVLDAQQVYELIKKNNVKQVYLLAALLSAVAEQKPKAAWKLNMDGLFV
                     MLDYCKENTGIKLFWPSSIAVFGPTTPRNLTPQQTIMEPSTVYGISKQAGERWCEYYN
                     QKHHVDVRSIRYPGLISYKADPGGGTTDYAVNIFHDAVLKNTYDCFLKENTALPMMYM
                     PDAIRATLELMNAPAEKIKTRSGYNLAGISFSPIELVEEIKKHIPSFTCTYTPDSRQQ
                     IADSWPASIDDSVAQKEWGWKHEYDLKEMTSEMLRHIRIKYNK"
     gene            19338..21002
                     /locus_tag="PKX59_05855"
     CDS             19338..21002
                     /locus_tag="PKX59_05855"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOZ90427.1"
                     /translation="MKQRVIYFSIYCLMALNCQAQLFDSAKPVFTHADSLRGYLSPIR
                     SCYDVKYYDLSVALNIPDESIKGSNSILFEVVSPTKMIQVDLTEKLSVASITDVHNNK
                     LNFSRDGNAIMISFPTTLNKGMYYKINIDYFGKPVVGKMLPWDGGFKWTKDEDGLPWV
                     AVACQGTGASVWWPCKDHQSDEPDSMMINITVPKTLMDISNGRLRKKTPVGNDMMQYS
                     WFVSYPINTYNVTINVGAFTHFNDWHINNNDTLTLDYYVKSYNLEKAKKQFEQVKPML
                     NCFEKYFGKYPFYRDGYKLVETPYLGMEHQSCIAYGNKYKTGYMGTDFSGIGLDFDYI
                     IIHETAHEWWGNNVTTKDIADMWVHEGFGSYAEVLYVECMYGKDKALNYVNAQRKKVG
                     NKTPVIGPYDVNTEGSGDMYPKGSLMLHTIRHYIDNDSLFFSILMNIQKTFAYQTITS
                     ADVEALMTKQSGKNLKPIFDQYLRHAAIPVFEYEVVEEKPFQLRYRWKADVAAFDLPV
                     RIDCNNENKRLNVTTQWQETTLAGFDSTCLIIRDDLGYFKVTKLNK"
     gene            complement(21051..22289)
                     /locus_tag="PKX59_05860"
     CDS             complement(21051..22289)
                     /locus_tag="PKX59_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90428.1"
                     /translation="MIRISTSIKLLIAFILPISTTAQVSETVTINPGYTNQTFYSMAN
                     ETISTIDNTDWDLAFQISGFEAAILINSKNNVKLFKANKDASEWSTMTAADTTGMYYS
                     ELLNNEAHIFNGAFNTTADTSDMFDLGWGEYDLGSHAVLGDSVYFIKLNNNTVKKIWI
                     ESLINATYNFRIADLDGSNEIQRSLQKLSYPAKNFGYYSIANDQFLDREPTKDSWDIC
                     FQQYVDHFINYKVTGVLTNDSVQSVKAYPVNDVTTATDAGYGYSYDNNLIGYDWKSFN
                     GSTFVIEDSVAYFVIDKNNHKWKMVFTGFGGSANGNFYFDKYDMGPLSVLENNSIETI
                     ALYPNPTTGSSQLIINSDNATTVTVDVTDLNGRLISSFAKKVNGLSNITIPSENFAKG
                     IYIVKINNGKQLQQLKLVVQ"
     gene            complement(22309..>22756)
                     /locus_tag="PKX59_05865"
     CDS             complement(22309..>22756)
                     /locus_tag="PKX59_05865"
                     /inference="COORDINATES: protein motif:HMM:NF025430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HOZ90429.1"
                     /translation="VYFSFDNGGKMVNIAPLKNQWDMMFTRYIHTYWDEPLQFRYYSV
                     NGVITNRWNNTTCAILKKDSLPNYMPFENFNHNNISAYPFHNTGNIIGFEWKFFNFND
                     NKYYITPDMYYIVKDKDEQYYKLKFYDFYNSTGVRGYPAFCYQRIL"
BASE COUNT         7291 a         4117 c         4108 g         7240 t
ORIGIN      
        1 aaaaagacaa agacaacacg aatgcttcac ctgaaacaga atatggcttt atcagtggtc
       61 gtgttcatac tcccaacaat accaacgttg gtagtgcatt aataattgcc ggtgaataca
      121 atactaaaag taatgcacaa ggtgaattta tacttcgtgt tccaacagga ttttataaac
      181 tcaaaataca aacaggcagt gggcatgtat ttaagtcaat ggttggtgtg gaagttaaag
      241 ctaatgacac cttgaggttg agtgattcag aatcgttact tcaacaaatt aaacaattag
      301 ctttcattcc gggagcttat gacagaatag aaaccattat cattgattcg cttggttatg
      361 ctgccacagc aatttctgtt aacgatttag acaatctttc ttacatgcaa agttttggtg
      421 gaatattttt tgactgtgga aaaagtggtt cattagacag tttaaaatac accaatataa
      481 gcaactattt agttaatggg ggtagtattt atgcttctga ttgggctata gaatacctaa
      541 ccggtgatgg aagctggaga cctgcattag gttcaaacac attaaatgta agtgcatcaa
      601 aaaatcaatc tcatgaagcc gggtacagta caatggcaac atgtttatca tcagaagtgg
      661 gtggctttat tcctgatagt gcattgtgtg catcaaagtc aggagcaata ggtttaataa
      721 caaatgcact gattaatgat ccggatattg tgacattatt aggcaaagat tctatggaca
      781 tagaatataa tttaggctca tgggagaaaa ttagtcatgt agatattcca tttacaacca
      841 tgattgaaaa aaacaatccc ggacctggac cgctcgctgt ttcatgtgac ctaaacggta
      901 cagcattcgg aggtcgtatt atatatacaa cgtttcataa tcatcctcaa ggaaacatta
      961 gtacagatgt taagttagtc ttacaacatt ttattctcag tctgtagcag agtaattcaa
     1021 cttttttaca aattattgtt tgcaagtcgg agtcatttcg aatattattg ctctgatttt
     1081 atttaaagga ttggcatcaa tcaactctct gcttttctac ctttgcggca gtttaaacaa
     1141 caagcatgag tagtcaagca gaatatgata ttgtagtaat tggtggagga tgtgtaggta
     1201 tggcatcagc ctataaactt aacctgcgtt tcccacatct gaaaattgca gtgctcgaaa
     1261 aagaagaccg ccttgctgca catcagacag gaagaaattc aggcgtaata cattcaggac
     1321 tttactataa gccgggatcc tataaagcaa aaaactgtgt tgaaggcagg cgtgagcttg
     1381 ttgctttttg taaagaacat caggtggcac acgatgtatg cggaaaaatt attgttgcta
     1441 ctgatgaaaa agaactgcca cacatgaacc gcgtttttaa taatggtttg caaaatggtg
     1501 ttgaaggaat tgaaaaaatt aatgcacaac agataaaaga cattgaacca ttttgtaccg
     1561 gcattgaagg catttgggtg ccctgcacag gtattgttga ttatgttggg ttgactaata
     1621 aactggcaga acttattgaa caaaaatttc cgggtagtaa agtttatacc ggccatgaag
     1681 ttactgcctt cgaaaaagaa gatggaaaca ccacagttgt taccaataaa caaaaattca
     1741 aaaccaagca tcttatcagt tgcacagggt tgttcagcga cagaatggca aaagcagatg
     1801 gcgtaaatcc taatatgcag attgtaggtt tcagaggtga ttactacgat ctgacagaaa
     1861 agggaatgca taaagtgcgc aatcttattt atcctgttcc taatcctgag tttccttttt
     1921 taggtgtgca ttttacaaga atgattcagc atggtgtaga gtgcggaccg aatgccgttt
     1981 ttacttttaa acgcgaaggt tatggtaaga ctgactttga tttaaaagac acccttcagg
     2041 cattagcata tatcggaaca tggaaactct ttttcaaaca ctggcgtttt ggtcttgatg
     2101 aatataaaag agctttctcc aaaaaattat ttcttgaaag actgcaaaga ttgattcctt
     2161 cattggagat ggatgacatc actcccggac gtgcaggtgt tagagcaatg gcacttgatg
     2221 caaatggaaa tatgattgat gattttaaaa ttgaatacag cggcaatgcc atacacgttt
     2281 taaatgcccc ttcaccggca gcaactgcat cgttagctat tggaacagca gttatggaaa
     2341 tggcagaaaa gcattttcag attcattaaa gcggatttta aaagttactt ttactgacat
     2401 aaacctatct gttttcagga aaattactca ttaccgcaaa tgacaattat cattttagtc
     2461 ccaacgcatt gattgacctt attctttaat tttgcagaga attattaact aattaataag
     2521 cattatgcgc actacaattc tccggtttac atttttagtt tcaagcatct tgttgtttag
     2581 ctttagcagc catgcacagg aaagtaaaac agacactatt aaagtttatg gtaactgtaa
     2641 tatgtgtaaa gaaaaaattg aaggctcctt aaaagagaaa gacggcatta ctgcaaaaaa
     2701 ctgggataag aaaaccaaac tactggcagt aacttataat ccttcaaaaa tcacaaaaga
     2761 acaaatagga caaaaaattg cagatgttgg ctacgataat cagtatgcaa cagctactga
     2821 gaaagtatat aaaaaactgc attcatgttg tcagtacgac aggcctaaac aacctgccgg
     2881 tgatgaaaaa aataaataga cactaaacaa acatatcaaa atgaaaaagc aactccttac
     2941 acttattctt tttgccataa catctttagg tatggcacaa acagcaacag actttacagc
     3001 aaacgactgt aacggcaatt cgcacaatct ctttactgaa ttaaattccg gaaaagtaat
     3061 tgttttatgt tgggtaatgc cttgtgcatc ttgtaccgga ccttctttaa caacctataa
     3121 tgttgttcag agttttcagg cttccaatcc taataaagtt tttatgtaca cctgtgatga
     3181 ttttgcaaac accaattgta cttctttaaa cagttgggca aacggaattg gtgttatgca
     3241 acaaagctgg tcgttacgat tttcgaatgc agcaattaac atgacgcatt atggttcacc
     3301 gggaatgcca aagattgtag ttttaggacc taatcatgct gtattttata attcgaacaa
     3361 tactgttaat gcaactaacc tgcaaaatgc tattaacaat gctttggcaa caacaggttt
     3421 aaatgaaagc aatttacctg catcatcatt atctgtactg ccaaacccag cacaaaacga
     3481 agcaaaaatt aaacttaacc ttctgaataa agctgatgta attctggagt gctataatct
     3541 tgaagggaaa aaagtacagg aaattttttc gggaaacctg actaaaggag aacacactat
     3601 tcctgtaaat cttgaacaat ttactgatgg catgtatctt atcaaatttt ctgacggtac
     3661 acaaacacag tttattaatc tgataaaatc caactaacag aataatagtt ttacagttgg
     3721 agtaaatttt gtaaattaaa tatctatgca taataaagtt caactgaaat tttctttggc
     3781 tctgttatta acttgcttct ccgtttctga taccttttcg caatgttgtg caggtggaag
     3841 tggcagccca attgccggag gaacttcaca aggtgttctt caggaaaaac aagttgaaat
     3901 aagtactaat tttcaattta tcagcaccga taaattttat aaaaaaaata aagttgcacc
     3961 tgattcagta cgaacatttg atggttttaa tagtacctat caatatttca gattggctta
     4021 tggtattaca aaaagcctaa cattttctgt tgagagcggc tattattatc aaaagaaaga
     4081 aacaggtctg cataaagatg cagccacaac atacaatgca aaaggttttg gtgatttaat
     4141 tctttttccg cgttatgata ttattaactg gacggaagaa aagcatcgta ctgaagttac
     4201 cgttggttta ggatttaaaa ttcccctcgg aagctataat gattcaacag gcaatgtaga
     4261 gccattttcg ggatacacct attatgttac caaccccaca tcggtacaac tttcatcagg
     4321 cgcacacgat attatttttt acacttttct ttacagagga tttacagaaa aaaatttcag
     4381 agtttttgct aatgccttgt acattaaaaa aggatataac cctaatggag aaaaattggg
     4441 tgactttgcc agtgtaggaa tttttgcagg taaaacattc ttcaacaact taggtgtaac
     4501 cttgcaggca cgatatgaat ggatggacag aatgaaagtt aatgaatcta tttctctgtt
     4561 tggaaaacca tcgaattatt ttcccaaagc tacgggttat aaaaagattt ttatatcacc
     4621 acaactcagc tataccaaag gtaagttcac ggtttatggt tcttatgaca ttcccgttta
     4681 tcagtactta aatacatcag aacactacac acaaatgggt tcacaacatc aggcaacact
     4741 tggtgttact ttccgcttta tggctttaaa tagttatcta aaagaaaaat caggagttac
     4801 ctatgtatgc cccatgcatc cgaatgttat ttcagataaa cccggtaagt gtcccgattg
     4861 cggaatggat ttggttaaga aaaaatagag ttacttttta ttattctgat tttatacttt
     4921 cacatataac tattcatcca acaaagacat gatcaggttg tttatttttg gcaaaaagaa
     4981 ggaatagtat tcataaatgt tatttgttca accatcatat ttaatcagac ttttttattc
     5041 aggattgtta tggcgtatgc caacgcgaca accaatagtt tacctgacat ttgatgatgg
     5101 tcctgagcct gaggcaacac ctttcgtatt gtcgcagtta aagaaatgga atgctcttgc
     5161 aacattcttt tgtattggaa acaatatcga gaaacatcag gatctgttta gtcgggttgt
     5221 ttctgcaggg catcagatag ggaatcatac acagcatcat ttgaatggat ggaagaataa
     5281 cctcaatact tatttggcag atgttgaaca atgcagcaca catatttcat ccggtttatt
     5341 cagaccccca tatggtaaaa taacacaaaa acaaattcgt tcactgcgtt cacaatggca
     5401 aattgttatg tgggatgttt tgtcttacga ctttgaaaca aatgtatcgc ctgaaaaatg
     5461 tattcagaat gtgatgcaac atgtgcgtcc agggtctatc atagtttttc acgatagtaa
     5521 aaaagcattt aaaaatttaa gtgaaacact acctgttatt ttggaaaagc tttcagcaga
     5581 aggttattct tttaaagcta ttcctcctta ccggccacta caataacaat ttcgccttta
     5641 ggtggatggt tggtataata ttcacagacc tctgctaaag tacctcgttt tttttcttca
     5701 aacatttttg aaatttcacg caccacacaa acctttctgt cagcaccaaa gttggttata
     5761 aaatcatgta gggatttaat aagcttatgt ggcgattcgt aaaaaaccat tgtccgcgtt
     5821 tcttcctgaa ggagtttaag gcgagtttgt cgtcctttct tagctggtaa aaagccttca
     5881 aaaacaaaac tgttgcaagg aattcctgac attaccaagg ccggaacaaa agctgtggca
     5941 cccggtagac aagtaagttc tatttcatgt tcaatacagg cacgtgtaag taaaaaccca
     6001 gggtctgata taccaggtgt cccggcatca ctgattaagg caattgtctt tttttcgtct
     6061 ttaagtaaac gtattaattc attgactgct gcatgttcat tgtgctgatg atgtgtaaaa
     6121 actttttttg aaattccgaa atgattgaga agtactgatg cattgcgtgt gtcttcaaca
     6181 agaataatgt cgcatgactt tagaatattt aatgccctaa gtgtaatatc ttccaggtta
     6241 cctacaggcg tgggaacgat gtataacatt catcaaaagt aaccaataga actttatttc
     6301 agtttttaat aaactttgtg ttataaacat gatcatcggt agtgatgacc tctaaaaaat
     6361 aaatcccgct tgccagttgt tccactgtta tccgatttga taagtcaaca gaataatttc
     6421 ttacttcttc accggttgga ttaaaaattc ttaccttttt aagaccaata tttgatggta
     6481 tattcaggtc aataaacttg tttgaagggt taggcataac acttagttta tctctgtcat
     6541 attcatttat accaacaaat ggcagaaata atacacaagc taaataagga ttatacttga
     6601 caaccgtgcc gccttgtgta cttacaggcc aaaccactac aacattactg cctaatttgt
     6661 aactagccac attgaaaaaa taaggatttt gtatagtaat gtttatactg ccattaggtt
     6721 gtatggtata taccgttccg gcattaagaa ctatagaatc ttcaggaagt gtgtcgctct
     6781 gaagtttcaa catcaatcca cctgtaaaag tggaattggc attataattc ttaatggaaa
     6841 cctgaatatt aaaataaggc tgttgcataa aggccgaatc aggacattga gggtactgcg
     6901 gagaaatagc aataacgcct aatgaatcta tttgtgctga agcagtgcca caaaacaaca
     6961 aactgaaaaa caacactatt atctgaaccc ttttcatgat gaagtatttt aaactaaaga
     7021 cttaataaaa gtataaattg ttttttaatc caagatactt atttttgtat tatcttacct
     7081 gcccaaaaag gctataatgg atattttaaa tcaactgata accggactta ataaagaaga
     7141 agtacgtttt tttaagatgt acatcaatcg tgttgagaaa actgacaacc gtaaagatgt
     7201 acaattgttt gattacatcc gtaaatcagg agataaattt tcagatgcaa ttatctttaa
     7261 aaaattatat caaccacaag aaaaaaattc ttactaccgc ttaaaaaaca gactcatcag
     7321 ggaggtaaat aaaagtctga ccctacagca ttttgatgac gacatggttg tatatatttt
     7381 caggttgctg gcattggtaa aattttatct aaataaaaat gctttaaaac cggcctattt
     7441 ttttctccgt aaagcagaac agaaggccaa tgatacagaa agtttagaac tgcttgatat
     7501 catttatggt gaatacatca ggctttcacg cgagatgaca agtattaatc ctgagattta
     7561 cattcatcgc agaaagcaaa attcagagaa gctcaatcaa ctccgtgtca ttgatgatat
     7621 actggcagta gttacctatc gtttaaaaat tactcaaaac ttatcatcaa aagacgatac
     7681 ggtaatttca ctgcttcaga aaactactga tgactttatc ggtgatccga aaattataaa
     7741 aagtccgctc ttccgtttca ggatttataa tgcaattagt cagttgcttt tacaatcgca
     7801 tcaatatgtt ttgttagaag attacctgtt aaaaacacta aaaatattta atcaggaaaa
     7861 acttttcact aaaaacaatc atgaaaccaa acttcaactg ttggtctatt tggttaatag
     7921 tctctataaa aacaacaagc taaaaaagtc gcttgaatac ataaacatgt tacatcagga
     7981 gattaaagca tataatcatc ttttctatga taaattttta ttttattact acaatgcgct
     8041 tgtgattaac tattctaaaa ctgataaaca aaaagaaata gtgaccttgc aagagatgga
     8101 acaaaatgaa aagattaatg ctaacttatt ttatcgtcaa tatattctgt tgaattttgc
     8161 tttggcatat tttgatcaga aagaatttag tatgtcagca aaaagtttta cccgattaca
     8221 aatgatacct gactataaag aaactgatga atcgctgcga atgaaagttg ccgttgcaga
     8281 acttatgaca cgctatgagt taaacgacta taacacgttt gaatacaaac tcaaacaatt
     8341 caataaagac tttagaagtg gcttaaataa acctgagaac gaacctgaaa aaataatgag
     8401 tgagatatta aaacaaatga tgtctgtaag taacagaaaa aacagagtaa ttgaattgcg
     8461 gatagcacaa ctcaaaacgc tatcgaaaga tgaagacaaa agtgaattga taaattattc
     8521 cgattggctg caggaaaaaa aattattgaa acataaaatc aggtagttgc ttccttttcg
     8581 aggcggtcaa tatctgcctg catggcagag ataagctttt caaactgttc tgcacattgt
     8641 tgtaacaatt cagttgcagc atttttttca gtcccctcaa gcatcagcca ttctatattt
     8701 tttaagattg atgcaaaggt atctaaatcg agttgtctga agcccggaat cattctgtga
     8761 acggccgctg ccgattgctg aaaatctgat tccttagcaa aatgaatact cttttcgatc
     8821 aaactttgat tacttgaaat aaaaaccttc aacattctga taacaaaatc gcttttacca
     8881 tttgatgtcc taaataagct atcgagagaa taacttaaca atggtttatg ctgcacctca
     8941 cgattcttat ctatcagagg taaaagaatt tcagatagtt catggagttt aaacggcttt
     9001 aaaataacag cattcattcc tgcggcaata cattcattct ttgcatcatt catcatattg
     9061 gcggtgatgc caattagcgg aagcatggca ctaatatgct cacgaataaa agaagtagca
     9121 ttgcgcccat caagttcagg catttgaata tccattaaca caccatcagg attatacaac
     9181 aaaatttttt caattgcctg tttaccactt tcagcagtta taacttctgc gccactatct
     9241 ttcagcatgg cagaaatcaa ttgtctgtta agcacatcgt cttctgctac aagcaaacgc
     9301 aatccatgca gtgatacaaa ctctgtttct gcaatattcg tttcagtctg ttttgtcaaa
     9361 ggaaaatgca gcatgatact tacagtagtt cccttttcag gaacactttt catggatatt
     9421 gttcctttct gcagctcaac taattttttt gtaattgcca gtccaagacc tgtgcctccg
     9481 taatttcttt caatattatc ggcttgttga taggcatcaa acaattttgc aatcttttct
     9541 tcggcaatac caataccggt gtctgttatt tcgaaattaa aaaaatcatc agcttgcttc
     9601 tttactttaa caaataccgt tcctttttca gtaaacttta ttgcattgcc aattacatta
     9661 atgagcactt gccttaatcg taatgcatca ccggaaacag taatattccg gatagtactt
     9721 gcttctaaaa ttaatgcaat atttttttcg tcagccttct gtcttaataa agttaccaca
     9781 tcatttactt ccttataaat aacaaaaggc tcttgtgaaa aatttaattt accggtttca
     9841 attcttgaaa gatccaatac atcatttaca atattcaaca ggtgctgccc tgccatgttg
     9901 atagtattca catattgtcg ttgctctgat gttgtgttgg tctgttgtag cctgttgata
     9961 aagcccgtca atgcacttaa tggtgttctg atttcatgac tcattgttgc cagaaactct
    10021 tctttgaatc gtgcaagttg ttctgatcgt gcccgtgctt cttcaagctg aattttatat
    10081 ctgtttgcac gactgatatc tgaaagaatt attatggaaa gtattaatat aagaactgaa
    10141 gctgtaattg ttgctattac aacagttttt aattgcttct gcgactgact gataacttcc
    10201 agcgacctgt tctcattgtt gtgtctgata ttttcctcga gcaatgcaat ctgtgattga
    10261 atcttttctt tcaacattct gtcttgctca agcaacaaca attcaccatc actgagttga
    10321 gctaccattt taatctcatt ctccttagct tcagagattg tttgtttgat ggtgcttaac
    10381 ttttgtgctt gtaaattatt taatgaatcg agcaacatag ctttttcttc aagctcttta
    10441 atctttttcc ctgaggaaaa cagtttctta aaaaatcccg gctttgaagt ttctacacta
    10501 tctgtattct gataaacatc tacatcctta accttatcag aaattttact cattgcagta
    10561 ttgataagct gtctgtaacg caactcaatc aacttatcgt aaatgtcaaa tttataatga
    10621 atagatgcag tgagtgagtc gaatgcctga ctgtttgccg gaaaaagatt ttttagttta
    10681 accaattgcg aatcggtttg ctcaacctgt ttataatagc tttctaaatt tttttcattg
    10741 cgtgtaattg caaaagagcg aacatgactt tctgcaaggt taacatttaa tataagctct
    10801 ttcaggtcag aaagtcttgt gtcgggcttc tgcagtagcg taattgttct gctgagtgct
    10861 gttgtattct gataattaaa ccaagccaga aaaaatgcta ctgccagagc aagcaacatg
    10921 aggataacta tatacaatcg tttctgcata atgagtcaaa aatacaacgc taaatctccc
    10981 aatggtggaa tatttttccc aaaatgaaac aatttatcag aaatcaaact tacatagttt
    11041 gttcgttaat aactttataa catattgatt ggtaaagtct ttaaatgctg aaaaacctct
    11101 ttattaaact caaacggaag cagtggcaca ttatttaata gtgcattgat gttaaacaat
    11161 ataaaatcat ttacaatgaa aaagctaatt ttaattgcat taccaatagt aggaatgacc
    11221 ttatttggtt gcgaaaacta taaagaacaa gctgcacagt taactcgcga gcgcgattct
    11281 ttagtatcac tgaatgttgc cggaacacaa actattgatg agtttatcac aactctgact
    11341 gatgtagaaa acagtcttgg caagattaca gaaaaacaaa attctattgc aatgactgca
    11401 gaccgtaatc cggaggcagc acgcaactca cgcgagagac tcaatgcaca gattgaagaa
    11461 atcaatcgta tgatgcagga aaacaaagat aaagttgagc agttgtcaaa aaaattaaaa
    11521 ggctcaaatg cctctgcagc aaaatttcag aaaatggtta agtcgcttca ggagcaactt
    11581 actcaaaaag aacaagaact tgctttgttg aatgaaaaat tagctggact gaatattgaa
    11641 gttggctact ttaaaaacaa acttcgattc tcttacaaca acaaatcaaa atcaggctac
    11701 tgtaattgct gatcaaacaa ctaaaatcca cactgcatat tacactgtag ggaactacaa
    11761 aagccttcgc gacaaacaag tgctttctaa aaaaggtggt gtattgggct taggtaaaaa
    11821 agcaactgta gtgcctgcaa ttaaaaacga taatttcacc aaaatcgatt atacccaaat
    11881 cagcactatt cctgtgaata gtaaaaaagt tgagttggta actacacacc ctgacggatc
    11941 atacaaaatt gaaaaaaata atgatcttgt tagcgacatt ttgattacag atcctgagaa
    12001 attctggagt gcatcaaaat atcttgtagt actgaccaaa taataaaaac agaaaataaa
    12061 atattctttg gttttaggtt gggtagcggg gcagcaatgc tccgcttttt ctttttaata
    12121 gatgaatgtt taataaaatt ctttacccct gtatgcaatg ttgcaaagtc attatacatt
    12181 tgcccatcct gacattaaga agttatgcaa aagaaatttt tcttcctgat attatttgca
    12241 tttggtttca taaaactcta tgcacaaaca gagcctgcta aaaccacaga aaaagaatcg
    12301 ttagttaact ggattagttt aagcgaagct gaaaaacata ataaggaagt tccaaagcct
    12361 ataattattg atttttatac cagttggtgt ggctggtgta aggtgatgat gaatacaaca
    12421 tattctgacc ctgcaatagc atcgtacatc aacgccaatt tctatcccgt taaatttgat
    12481 gcggaaacaa aagatacaat tgagtatgat ggtgtaaaat acaagcccac aggaactgaa
    12541 aaacgcgatc ccaatgagtt tgcagtaaaa atgctgaatg gcacaatgat gtatccttcg
    12601 actattttta tgaataaagc tgcaaacttt accatgtcgg cacagggata tctggaaacc
    12661 aagaagattg aacctatgtt ggtgtttaca ttagaaaatg gctttcgcaa cagtagctat
    12721 gaagatttta acgcacaatt tcaaaaagct ttttatgatt cattgcaaac caagtcttac
    12781 gaatctgtta aatggcaaac accattacag ttttttataa aagacaaaaa acctgatcag
    12841 aaaaagaaaa ttatatttat aaatacagaa tggtgtaata cctgccgtgt gatgtatata
    12901 actacattta tcgatactgc tgtgagtagt ttattatcaa aacattttga attggtaaac
    12961 ttcaatcctg aaactaacga caaactttat tttcaggaca aagaatttga taacatacat
    13021 tcaaaagaat tgccttttca tcagttggtg tatgcactaa gtcgtaacgg attattattt
    13081 ccgcaagtga tatttatgga tgaaaagaat actgttgttg acgctattcc gttttatctg
    13141 aatccaaatg tttttaaaaa tattgttcgt ttttacggag aagatattta taaaacaaaa
    13201 aactgggaga cttttattaa agagcaggaa acaaaataat tcctcgtaat tatcctacac
    13261 aatatttaaa aacctaataa cacaaatcgt caacgtatga aacgtttaaa aaatcctgtt
    13321 acatttattt tcattaccgt tttagttgat tgcatcggta ttggaattat ttttcccgta
    13381 attgcatcac tgataacaga ggttagtcat gttccagtaa ataaagcaac aacctacagc
    13441 ggctggatga tgacccttta tgcattgatg cagtttattt tttctcctgt gcttggtggc
    13501 atcagtgatc gctatggtcg caggccggtg ctgcttttgt cgttgttagg tttaggtatt
    13561 gattacttat ttttatctgt agcacataca ctaacactat tatttatcgg tagaattatt
    13621 gcaggaatat gtggtgcaag ttttacaaca agttttgctt acattgccga tgtcagccct
    13681 tctgaaaaaa gagcgcagta ttttggtatg atgggtgcag cattcggtgt aggttttatt
    13741 attggccctg ttcttggtgc attggcaagt cactggggcg tgcgtgcacc atttgttgta
    13801 gctgcttgtt tatcattgct gaattttcta tatggatatt tcattttgcc tgaatcttta
    13861 aaacccgaaa acaggcgagc ctttcattgg aaacgtgcca acccacttgg tgctttcatt
    13921 cagattaaga aaagtaagaa tatcagaacg ctcatcttta ccatgtttct gctttatctt
    13981 gccggacaag taatgccatc tatctgggcg ttctacacca agtttaccta tcaatggagt
    14041 gatgcagaga ttggatactc attagctttt gttggtgtga tggttgctat cgtacaaggt
    14101 ggactaatta aatggtcgca gacaaaattg ggcagtcaca cttctattta tacaggactt
    14161 gctttttatc tggtaggatt aaccttgttt gcctttgcca accaatcgtg gatgctttat
    14221 gcatttactt ttatttatgc attaggaggc attggtccgc catcaataca gggaatcata
    14281 tctggcttag taccacctaa tgagcaaggc gaacttcagg gaatgatgac tgcactgatg
    14341 agtgtgtcaa caattttatc gccattgata atgaccaatt tgttttacaa tttcaccaaa
    14401 ccggatacga tatattattt tcccggagca ccatttgcag cagcagcaat tttagtgttg
    14461 atttcgtttt ttgttgtgaa gaggggaata aataatctga cccgccatca gaaaatataa
    14521 atataatatt taatgataca gatttttaat ctgtaaaata ttgcaacacg aattgttcaa
    14581 taaatcttct cttcactata aaacgactta tatcatttta ctttttggag gctgctttat
    14641 tggctttagg atgcacgtct aatttcaaca gaggcgagtc tgatgtgtaa ggtactttcc
    14701 ccgaaggaac cacttttgac gattccgata aatgcacttc ctgatcatca aaaaatatat
    14761 gtgcaccaaa tgcttttaaa acattatctt ttgaaagccc tcctaaaaaa tatgcctcat
    14821 caacatatac accccactta cgaagtgttt taatgacacg aatgtgtgag ggtgcatttc
    14881 gtgctgttac aattgctaaa cgcaatggcg aaagctcaat tggaggaggc atctcatcct
    14941 gaattttaga caatttgatg agcaacttcg caaatggacc ttctgccaat ggcacatcct
    15001 gatgttcttg ctcgtactta tgaaatgcct ctattccttc tgttttatat ctgtgttctg
    15061 attcatcaga aaacaaaaca gcatcggcat caaaagcaat ttttactctg ttatctgttg
    15121 gcttaaattc tgttggtggc tcgtaaatat atgcagctgc tgattttttt gaatcaataa
    15181 cattttgtac atctttcaaa tctttgctca gaaataagtc aacatcaaag gcatcaatat
    15241 agggtgctaa aggttctcca ccactgaaag ccattcgcgt aatatccaat ttatatttcc
    15301 ctacagaatt cattaccctg actcctgttt ccggactgtt tctagacata acaatcactt
    15361 caacgattct cctttttgca ctctcattca ggtgaagcaa actctgaaca agatgaaaag
    15421 cagtgccagg ttgcagaggc tcatgttcat gagcaagctg atattttcta aaccctgata
    15481 ttccttcttt aagaaatatt tcattctctt tttcgaggtt aaaaagcgct cggctggaaa
    15541 caccaactac taatatttca gaaaaattta ccggcattta cttttgattt atctatcaat
    15601 tcaatttgaa tgaaacaact ttataagtat ttaaatatat aaaccttact tactgaaaat
    15661 tgtttgcatc actacccttc caaccatgtt taatgtgttt ttatcaatga tggaaagatt
    15721 gtcgccatgc gtatgatgat aaggtggata ttctttcttt acattatcgt aatgcactat
    15781 gtcaatggtt ctgatattgg caagttgatt gatgtataaa tggtcatcta ttgtttgtgg
    15841 ggcaggttca ttaataaaaa attgtccata acccatgttc tgagcaatgt tccaaacttt
    15901 actcaacaca tcaggtgcat aatacaatga tgttccttcc tttggaaaaa caggattaac
    15961 accaccaacc atatcgagaa gtattccgta ctgagcaaaa taatctgagc gatgcatatt
    16021 ctttgcccag aattgtgaac ctaagcacca agtattttct tgccgaggaa aacgtgtgtc
    16081 gtcttcttcc tgtccgtaat cttcaaggtc aaccaataaa atatctattc ctgtttcaag
    16141 tcctgatgca ggcagtagtc ttgcaatttc aagcaatact gccacaccac ttgcaccatc
    16201 gtttgcacca tcaaaaggtt gatcctttcg cactgaatca tgatcggccc aggggcgtgt
    16261 gtcccaatgt gccagcaaca aaactctttg ttttgcctct ggtttatatt cagcaataat
    16321 atttttcagt ctgaattttt tactgtcgaa tgtagttatt gtttcagact gaatctgtgt
    16381 ctgcaaacca aatgacttta gtttttgaaa aaggtaatca gcgcattgtg catgtgcatc
    16441 ggttccgggc acacgcggac caaatgcaac ttgtgtctgt aaataatgta aagcagagtc
    16501 agcattaaat gccggcacag gaatatttac agtagcagga gtttcctgtt ttgtctctgt
    16561 gctttcttgt gcaggctgtt tacacgaacc caccacaagt aatacagcaa cagcaattaa
    16621 atttaaaaaa ttacattttt tcccaagtgc tgccatcttt cgagtctttt acatttattc
    16681 ccattgcatt taaactgttg cgaatatggt ccgaaacaga aaattctttt tttgcttttg
    16741 cctgattgcg catttcaaga accaattgca ttacattatc aagcacagac tgatcgcctg
    16801 aagaaaccgt atcatccatc agtcctgcaa tgtcaaaaac aaaatgctga ataattgatt
    16861 ttgcctctgc aatatcttct ttcgtcatct tttcttttcc atcattagcg gagttgatta
    16921 tccgaactaa ctcaaacaac tgcgctacca taaccggact gttgaagtca tcgttcatgg
    16981 catcgtaggc attttttctt attgaagata aacttacaga ggtaacgtct gaggccttta
    17041 cgctgttgag ttttttaacg gcagacatca atcgctgata tcctttttct gctgcctgca
    17101 gtgcttcatt actgaaatcg agcgtacttc tgtaatgcgt ttgcatcata aaaaagcgaa
    17161 ctgccattgg tgaataaccc ttttcgagta aaggatgatt gcctgtaaaa agttcgcccg
    17221 gaagaaaacc attacctgca gattttgaca tgcgtgtacc gttgaccgta agcatattgg
    17281 tgtgcatcca aaagcggcat ggctgcacgt tgctgcatgc ctgcgactgt gcaatctcat
    17341 tggtgtgatg tgtagctgca agatccattc ctccaccatg aatatcaaat gtggcaccga
    17401 gatattttgt actcattgcc gaacattcaa tatgccatcc cggaaaacct acaccccatg
    17461 gcgatggcca tcgcatgatg tgttcgggtt ttgcatttat ccacagtgca aaatctaatc
    17521 gtcctttctt ctcatcctgt ccgccaagtt cacgagtgtt ggcaatcata tcttccaaca
    17581 gtcggttggt gagctgaccg taattaaatt ctttactgta tttctctacg tcaaaataga
    17641 ctgtgccatt cacttcataa gcataaccat tgctgatgat ttgcttaatc atttcaatct
    17701 gctcaatgat atgtcctgtt gcagtaggct caatgcttgg tggcaaggtg ttgaatgcac
    17761 gcatcacatc atgaaaacct aaagtatagc gctgcacaat ctccattggc tctaattgtt
    17821 ccaagcgtgc ttttttagca aacttatcat caccttcatc tctgtcgcct tcgaggtggc
    17881 ctgcatcggt aatgtttctt acataacgta ctttatatcc caaatgcaaa agataacgat
    17941 aaatgagatc gaaagaaata aaagtacggc aattacctaa gtgcacatca ctataaacag
    18001 tagggccgca aacatacagt cctacgaatg gtgggtttag tggttcaaat ctttctttct
    18061 gtcgcgaaag tgtattgtaa atattcagcg gttgaaattc catgtttatt tattgtattt
    18121 tattctgatg tgcctgagca tttctgaagt catttctttg aggtcgtact catgtttcca
    18181 tccccactct ttttgtgcaa cactgtcatc aatgcttgca ggccatgagt cggcaatctg
    18241 ctgacgtgaa tcgggtgtgt aggtgcaggt aaaagaaggg atatgctttt ttatttcttc
    18301 aaccaattca atcggagaaa aagaaatacc tgcaagatta taaccactgc gcgttttaat
    18361 tttttctgcc ggagcattca tcaattctaa tgtagcacga atagcatcgg gcatgtacat
    18421 cataggcagg gcagtatttt ctttcagaaa acagtcgtaa gtgtttttta aaactgcatc
    18481 atgaaatata tttactgcat agtctgttgt tcctcctccg gggtcagctt tgtaacttat
    18541 cagaccggga tagcgaatac ttcttacatc aacatggtgt ttttgattgt aatattcgca
    18601 ccagcgttca ccggcttgct tactgatgcc atagactgtt gacggctcca tgatagtttg
    18661 ctgtggtgtt aaattacggg gtgtagtagg gccgaaaaca gcaatggagc ttggccagaa
    18721 taattttatg ccggtatttt ctttacaata gtcgagcata acaaaaagtc cgtccatgtt
    18781 tagtttccat gctgccttgg gtttttgttc tgccactgcc gacagcaatg ctgcaagcaa
    18841 ataaacctgc tttacgttat ttttctttat cagctcatac acctgttgtg catccaacac
    18901 atcggccata tagaatgggc cctttgcttt aaagtcggca ggctgatctt ttatgtcagt
    18961 tgcaataaca ttgtccgacc catatattct tctgagttct tctaccaggt ctgttccaat
    19021 ctgacctgcg gaacccagta caagcgttcg ctctgtttgt gacatagttt acaaatatct
    19081 gtgaaaggac aaaagtaaaa tttctaagcg tttgtttaag tggttattta attaatagct
    19141 tacggctgaa accttgggct attatttata gaatcaaaga taaatattca cctactattt
    19201 tacacccaaa ccatttaaag cgtaagaata ttgtttcaat aagttttatg cttaatctgc
    19261 aagaattaaa aatgcttttg caatttctta actattaatt acgatttgct ttctactttt
    19321 gaaaaaaatc ccgaacaatg aaacagcgag ttatttattt ttcaatatac tgcctgatgg
    19381 ctttaaattg ccaggcacaa cttttcgata gtgctaagcc tgtatttacc catgccgatt
    19441 ctttgcgtgg ttatttgtcg cctataagaa gttgttatga tgttaaatat tatgatttat
    19501 ctgttgcatt gaatatccct gatgagtcta ttaagggcag taacagcatt ttgtttgaag
    19561 tagtaagccc gacaaaaatg attcaggttg atttaactga aaagttatct gttgcctcta
    19621 ttactgatgt acataataat aagttaaact tcagtcgtga tggcaatgct attatgattt
    19681 cttttccaac aacattaaat aaaggcatgt actacaaaat aaatattgat tatttcggga
    19741 agcctgttgt tggcaaaatg ctgccttggg atggaggctt caaatggact aaggatgaag
    19801 atggtttgcc ttgggtggct gttgcctgtc aggggacggg tgccagtgtg tggtggccgt
    19861 gcaaagatca tcagagtgat gaacccgata gtatgatgat aaatattact gttccaaaaa
    19921 ctttgatgga tatttcaaac ggaaggcttc gtaaaaaaac tcctgtgggt aatgatatga
    19981 tgcagtacag ttggtttgtg agctatccga taaacactta caatgtgaca ataaatgtag
    20041 gcgcctttac acattttaat gattggcaca taaacaacaa cgacacttta actcttgact
    20101 actacgtaaa atcatacaac ttagagaagg ccaaaaaaca atttgagcaa gtgaaaccca
    20161 tgttgaattg ctttgaaaaa tattttggga aatatccttt ttaccgcgat ggctataagt
    20221 tagttgagac accttacttg ggtatggagc atcagagttg cattgcctac ggaaacaaat
    20281 acaaaaccgg atatatggga acagattttt cgggcatagg attagatttt gattacataa
    20341 ttattcacga aactgcgcat gagtggtggg gaaataatgt gactacaaaa gatattgctg
    20401 atatgtgggt gcatgaaggt tttggcagtt atgcagaggt gctctatgta gaatgtatgt
    20461 atggaaagga caaagcctta aactacgtca atgcgcaacg aaaaaaggta gggaataaaa
    20521 ctcccgtcat tggtccttat gacgtaaaca cagaaggcag tggcgatatg tacccgaaag
    20581 gaagtttgat gttacacacc atcagacact atattgataa tgactctttg tttttttcta
    20641 ttctgatgaa cattcagaaa acttttgcct atcaaaccat cacttctgca gatgttgaag
    20701 cactgatgac taaacagtca ggcaaaaatc ttaaaccaat atttgatcaa tacctgcgcc
    20761 atgcagcaat tcctgttttt gagtatgaag tagtggaaga aaaacctttt caactacgtt
    20821 accgctggaa ggcagatgtt gctgcatttg atttgcctgt aagaatagat tgtaataacg
    20881 aaaacaaaag attgaatgta actactcaat ggcaggaaac aacacttgca ggttttgaca
    20941 gcacctgctt aatcattaga gatgaccttg gctattttaa agttacaaaa ctgaataaat
    21001 aaaaaaggct gccaataaca gacagcctga ttattaaaat caactcaaaa ttattgtaca
    21061 accaatttaa gttgttgcag ttgtttgccg ttgttaattt ttacaatata gattcctttt
    21121 gcaaaatttt ctgaaggaat agttatgttg cttagtccgt ttaccttttt agcgaatgat
    21181 gaaatcaaac ggccattcaa atcagtaaca tcaacagtaa ctgttgtggc attgtcgctg
    21241 tttataataa gttgcgagct tcctgttgtg ggattaggat agagagctat ggtttcaatg
    21301 ctattgtttt ctaaaacaga caaaggaccc atatcatact tatcgaaata gaaattacca
    21361 ttggcagagc caccaaatcc ggtgaatacc attttccact tatgattgtt tttgtcaatg
    21421 acaaagtaag caacagaatc ttcaataacg aatgttgacc cgttgaaaga tttccagtca
    21481 tagccaataa ggttgttatc gtatgaataa ccataacctg catcggtagc agtagttaca
    21541 tcattcacag gataggcttt aacagattgt acactgtcgt tggtgagcac gccagtaact
    21601 ttgtagttga taaaatgatc aacatattgc tgaaaacaaa tatcccaact gtcttttgtt
    21661 ggttcgcggt ccaaaaactg atcgtttgca attgagtagt aaccgaaatt tttagcagga
    21721 tagcttaact tttgaagtga gcgttgaatt tcattgctgc catctaaatc ggcaatacga
    21781 aaattataag ttgcattgat aagagattca atccagattt ttttaactgt gttgttattt
    21841 agttttataa aataaaccga gtcgcccaat acagcatgtg agcctaaatc atattcgccc
    21901 catccgagat caaacatatc cgatgtgtcg gcagtagtat tgaatgcacc gttaaaaata
    21961 tgtgcttcat tattcagcaa ttcggaataa tacatgcctg ttgtatctgc ggcagtcata
    22021 gtgctccatt ctgaagcatc tttattggct ttaaatagtt ttacattgtt ttttgaattg
    22081 atgagaatgg ctgcttcaaa tccagaaatc tgaaatgcaa ggtcccaatc ggtattgtca
    22141 atggttgata ttgtttcatt agccatactg taaaaagtct gattggtgta gccgggatta
    22201 atagttactg tttcactgac ctgtgctgtt gttgaaatag gcagaataaa tgctattaat
    22261 aatttaattg atgtagaaat gcgaatcatt tgattgtata tttaaaattt ataaaatacg
    22321 ttgataacaa aatgcaggat aacctctgac acctgtacta ttgtagaagt catagaactt
    22381 tagtttgtag tactgttcgt ccttgtcttt aactatataa tacatgtcgg gagtgatata
    22441 atatttattg tcgttaaaat tgaaaaactt ccattcaaaa ccaatgatgt ttcctgtgtt
    22501 atgaaatgga tatgctgaaa tattattgtg gttaaaattt tcgaatggca tgtaattagg
    22561 taacgaatct ttctttaaaa tagcacaagt tgtattgttc catctgttgg taattacacc
    22621 atttacagag taatatctga attgcaatgg ttcgtcccaa taggtatgga tataacgggt
    22681 aaacatcata tcccactgat tttttagcgg agcaatgttt accattttac caccattatc
    22741 aaacgaaaaa taaacc
//
LOCUS       DAOOOI010000033        22350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_434_length_22350_cov_23.034851, whole genome shotgun sequence.
ACCESSION   DAOOOI010000033 DAOOOI010000000
VERSION     DAOOOI010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 22350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22350
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_434_length_22350_cov_23.034851"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /gene="murI"
                     /locus_tag="PKX59_05870"
     CDS             complement(<1..706)
                     /gene="murI"
                     /locus_tag="PKX59_05870"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOZ90430.1"
                     /translation="MTVNQPIGIFDSGIGGLTVAAAIGRLLPQEQLIYFGDTAHVPYG
                     DKSVELIGSYAERITEFLLHEQKCKAIVIACNTASAAAYSLLRDRYKGSVPVINVIDP
                     MIEAVIADDDIISVGIIATKTTIASGTYQEKFSRRKPQLLFHPLATPLLASMIEEGFY
                     NDNISSAVLEEYLSYEPFKSIDGLVLACTHYPLIKKEINHFFQSKVKLFDSAEVVASK
                     LKKILEAESLISLERKK"
     gene            complement(703..1689)
                     /gene="dusB"
                     /locus_tag="PKX59_05875"
     CDS             complement(703..1689)
                     /gene="dusB"
                     /locus_tag="PKX59_05875"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOZ90431.1"
                     /translation="MVNIGSVKLGEFPLLLAPMEDVSDPPFRFVCKQHGADLMYTEFI
                     SSEGLIRHAAKSTHKLDIFEYERPIGIQIFGSDVESMRQAAIIAEQAHPDLIDINYGC
                     PVKQVACKGAGAALLQDIPKMVSMTKAIVDAVKLPVTVKTRLGWDDKTKNILEVAERL
                     QDIGIKALTVHARTRSQMYKGSADWGLVGELKNNPRLTIPVFGNGDVDSPQKALEMKN
                     RYGVDGIMIGRASIGYPWIFNQIKEYLAGNCAIAVPGIAERVEVCRTHLIKSVEWKGT
                     IVGILEMRRHYTNYFKGMDHFRDFRVKLVTSNSLNELLDLLEQIKIKYKEAA"
     gene            1744..2658
                     /locus_tag="PKX59_05880"
     CDS             1744..2658
                     /locus_tag="PKX59_05880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOZ90432.1"
                     /translation="MIKGKWQHSDPLLKMLLFCGIVMTCFGLISVLNIPLVKIFFGKS
                     LPEVMSGFSSGDTTYINAMKFIQIFSQLGLMLVPAWLCGWLFSDNVIQFFQFKKINFI
                     QIVVIVVVTICLSPLINLLLDWNSHLQLPASLAAIEEMMRNMETEAEKLTESFLVMHT
                     NFDLIINLLMIALLPAIGEELLFRGVLQKLIQQATKNKHWAVIITGIIFSAIHMQFFG
                     FLPRFMLGIFLGYLLVWTGSIWAPILAHFINNGSAVLLSYYEQKNAINIDEEKLGIRE
                     GEYWMVFASVIITVSACYILYRSKQKSS"
     gene            2780..11503
                     /locus_tag="PKX59_05885"
     CDS             2780..11503
                     /locus_tag="PKX59_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ90433.1"
                     /translation="MPRIIAFCVLYFVANVTLAQDIFMSNGVVSGCSGTFYDSGGDST
                     NYSNNENFVYTICSDQPGKCVQLSFQSFAIEKDFDFLFIYNGPSTSSPLLGTFTGTNS
                     PGTITATSGCLTIRFSSDYTVNRGGWKAYVNCINCPVNGCPTCNGGAPPPNDACSGAQ
                     NLGLLPQPAACPNGIGAVNLTNTSNLCATAEIPYQSLQGCKPIGSMSNPATDVWYKFS
                     LTAPVLDITINGLITPQVGLYSGTGCGNLVPRGCAIGGGGYLHTTFGGLAAGTYYLQV
                     SGGNINDQCNFSLSLQNNFDCSGCVMQSGLTVSPPPSNGFYLSGQQVTFCLNVTDFNP
                     TSANWLHGIVPKFGPGWDTTTIIALPPASCSNNGFWSWYSNPIISSANNKPYGPGFFY
                     ETSAGNSSNTQDNNPGNNFGDNLGQNCNLNFCWKINTLPQSLCNFGTNLNVFIETYTD
                     GESGSWTSAACANDPVHDLYATLACCIPPVVAITQPVCFGTQGSAIGTAQGSGPWTFI
                     WKNSSGVIIQQNGPVNGIDLISNLNPGTYQLITEDVNGCQSTVSFAVTQPQPLTATLA
                     VSATKCGLVNGSINITASGGTAPYSYSINNGVSFQGSPQFSNLNSGNFIITIKDAHNC
                     TVSVPAAVNGSTVPVINSLTSQNVTCYNGADGEVTVTASSGIAPYVYELKKSGLTLSQ
                     SSSIIQNVPAGTYQLTVTDVNGCKSDTTVTLTQPPQINMVSVITPSGCNLNDGTIELT
                     ITSSFSGTLLYSIDNGTNYQTGNLFSNLSPGVYNVVVEDGNGCMIKDTAIVNTINAPN
                     IDSVKSQSLTCYRTQDGAINIFGSGGIGTLAYSINNGSTYSATNSFNTLIAKTYTVVI
                     KDNNNCRVYQLAAVKEPPPLIIRGDIIATSCGLNNGSVNLHTDNGILPLEYSTDGGLT
                     WSTNYYYGQLPTGTITFTVRDANGCTSTRPYQIAASTAPSFDNVSVQSLKCFNTNTGS
                     ITLNSINGIPPITYSIDSGATYQPTNQFQNLISGNFYLSIKDNKGCRADTLVQLSQPI
                     PLSFTSAVTNTICSYPNGTIDITGNGGTIPYTYSIDGGATFNGNSNNLNLNPGSYKIV
                     VRDINNCEEASTVSIIDEPGPRITGHNFTPQICDNAANATVTINAISGTGTLFYSADS
                     GLTYSIANNINNLIGGSCTLIVRDANLCTDTVAFNIPILHSPQIDNNATTNPICFGSN
                     DGAIQIVASGGNGNLNYSIDNGVNFFTNSTFNQLNAGTYYLTVEDSNHCQVKDTVLLT
                     QPPLLTAQTIVSNETCSNQNGVVSIIAGGGTPTYYFELSNNTTATDTFFTQLGAGNYS
                     ITTTDYNGCTTQAVFQINNLDKPTISQTALTNLSCYQSEDGVIQLAALGNGTMQYSLD
                     GIIFQNSNIFNQLSAGSYTITVRDTNGCTADTTILLTEPNAITANIQTTSSNCTLSNG
                     SLTVNASGGSGNLSYSLNGGTFNSQTIFNNLSSGNQSITIQDANNCQAAFVASINTIN
                     GAVINNISTVDIKCHGQQTGEITITASGGTGPLSYSIDNGISSVSSNHFTMLAAGNYN
                     IILTDSVGCITSSNIILNEPDSIIIQQAVTAATCNQNNGSFQIVVNGGTGILQLSLDS
                     INFSPGTIFNNLIAGIYQLHVTDANQCYATKLISVPNQAAPVLTNLSKGDVTCFGDST
                     GFISLSASGGTGLLNYSIDNGVTYQALPNFNNLPTGSYHIKISDQNLCSTDTIIQLTS
                     PDDITATLTITNANCQQSNGSITVNAQGGNGTLLFSLNGGPFSQQTYFGSLPAGTQQL
                     QVKDGMMCDKNIPVQINTINGPTLDTVITQNLNCFGINNGTISILASGGTGALGYSLN
                     NQSFLNTPNFNQLSAGNYHVSVVDSAGCLVSGLVQLTTPSAIQVTPTVTNPACGQNNG
                     SLNLQLAGGTGTLLTSLNGSSYNNITNYSNLYAGSYTLKIKDANNCEVQRAVSLSNIL
                     APKLIGSVLKQVKCFNANDGEIKILASGGTGVLTYSIDNGITTQISNTFSNLTAGTYS
                     ASVIDQNNCRSDTTITLLQPDSLQLLLNITPETCSAGNGSITVSSTGGTSPYLFSSDS
                     GQTFYNVAGFAQLDSNIYYIIVKDSKNCRTLNQVNITNLTGPQIVSLTTTNVSCYGLQ
                     DGAVQIATAGGNGTLSFSTNNFITQQTDTFFNALLPGNYTVYVSDTNNCKSNHFFSIS
                     QPTVVQSNIVSTQPSCFGYNNGSLIVTANGGTPPYNILWSSGSTDFNLSGIVSGQYSF
                     IITDAHNCNKSDSIYLSSPTVLAANHTTQNGTCSGNANGSALVSVTGGTPPYQYNWSP
                     IAGNTNYANALPAGIYTVDVTDKNNCTLTEQIQISTPAPIANTFAKNDVSCFGGSNGM
                     ITANALGGTAPYSYLWSYQQQTNDTILNLPVGSYSVTITDANGCDNFFTEQISSPQQI
                     KPNSIINNVSCNGFNDASVTLNVQGGVAPYSYQWNNGVTQSLNTNLAAGIYVVTVTDA
                     NNCNRVKAFTITEPLPILLSIYPVDTICIGQQVLLNAIASGGTGTYNWLWNNQAAAST
                     ISVTPSVTTNYFVSVTDSNNCPSKTDSILVIVFPPLGITATGYDTICAGQSAVLSSIA
                     NGGNGGPYSYLWSNGSDSATVNVSPSVTTTYTVSITDNCTQMPATAMVPVVVNETPSL
                     DVSPMTEQGCMPLEVQFLNSSTYPQGTIFTWNFGDGQTTDSITPLHIYNADGIYTVSV
                     QAITPDNCVSNIIISNPIEVYPLPVASFNISPESISILHPEINISDSSLLGSIWFYDF
                     GDGQTSTLQNPNHIYQDTGRFTVIQIVESEFGCRDTSKREAIVEGVFTVYIPNAFTPN
                     NDGSNDYFFANGYGITELTMQIFNRWGNKVFETPSVTGRWNGIDTSGKICPQGVYVYT
                     AKIKDQFGIYHTYNGQVTLIR"
     gene            complement(11496..12782)
                     /locus_tag="PKX59_05890"
     CDS             complement(11496..12782)
                     /locus_tag="PKX59_05890"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HOZ90434.1"
                     /translation="MAEIVRMPKMSDTMTEGVVAKWHKKIGDQIKSGELVAEIETDKA
                     TMEFESYQNGTLLYIGVEEGKAAPVDSILAILGKPGEDFKPLLEAESKAKTATAAVPE
                     KTEAKPAAKVVAETPVKTVAAPKVEAKPVTQPVVASESDSRVKASPLARSLAGQKGLD
                     LTMIKGSGDNGRIVKRDVEWFKQGNVVAGSYTSAIRTQESFEETPVSQMRKTIARRLS
                     ESKFTAPHFYLTLDIEMSRCMDAREAINAATGSKVSFNDFVIKACAAALQQHPKVNSS
                     WLGDTIRTNHHIHIGVAVAVDEGLLVPVVRFADSKTLSQINAEVKTYAQKAKDKKLQP
                     ADWEGNTFTISNLGMFDIEEFTAIINPPDACILAVGAIKAVPVVKDGSVIAGNVMRVT
                     LSCDHRVVDGVTGAKFLQTVKGFLENPVLLLGQASI"
     gene            complement(12811..13809)
                     /gene="pdhA"
                     /locus_tag="PKX59_05895"
     CDS             complement(12811..13809)
                     /gene="pdhA"
                     /locus_tag="PKX59_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HOZ90435.1"
                     /translation="MAKQKFTKETYLFWYEQMLLMRRFEERSGQLYGMQKIKGFCHLY
                     IGQEAVVAGAMSVIKPTDNMITAYRDHAHALGKGTSARAVMAELFAKATGCSKGKGGS
                     MHLFDAANRFFGGHGIVGGQIPLGAGIAFAEKYKGTDSVTLCYMGDGAVRQGAFHEAL
                     NMAMTWKLPVVFIIENNNYAMGTSVERTSNVTDLYELGTAFNMPSEPVDGMMVETVHE
                     AMDKAVSHCRKGNGPYLLEMNTYRYKGHSMSDPAKYRSKEKLEKYKAQDPIEEVLKVI
                     QKNKLATQEEIDAIDKKVHDIVEDSVTFAEESPFPDVDELYKDVYEQSDYPYIIED"
     gene            complement(13850..14344)
                     /gene="cdd"
                     /locus_tag="PKX59_05900"
     CDS             complement(13850..14344)
                     /gene="cdd"
                     /locus_tag="PKX59_05900"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOZ90436.1"
                     /translation="MMQKIQLTSSYEEYNSLNELPADYKSLMLEAHEAAETAYAPYSD
                     FHVGAAVLLANGEIIKGSNQENAAYPSGLCAERVAVFASAVQYPGVKIIAIAVTAMPG
                     SQEEPVMASPCGACRQVMAEYEHLYHQDIKFIMYGSENKIIVLDSIKALLPFSFNSDQ
                     LSGK"
     gene            complement(14459..15805)
                     /locus_tag="PKX59_05905"
     CDS             complement(14459..15805)
                     /locus_tag="PKX59_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90437.1"
                     /translation="MKALAGYFSLWLKKNTFLLWALAVIHLIFFLLALRNGNIAFSVD
                     STEYLNQAENIREHASWYAGSSSTPHDDFLESRRPPLYGFFIFLVHIVSSNNFFLLAL
                     QNLMSIAVLILTAFMFWLLTGKRIANQWLVILPLLLFPTQFIYANMIMADVLFQFLII
                     SGFLFFIQSVRRPDSFLYFCIFISLAILTKPVFYAFCLIVPVIALLFYTKGIYKLSNV
                     YISLIPLLTVATISTINYSKTGYFHYSSVNEKFISEYGAYLAVGDKGDISAQQKIDSL
                     LSEANKQPDFKSYCKYINKESLFLIKENKARFVMMQLKGMANFFIDHGRFDLMAFFVN
                     PDYVQTEGWKQKYEEQGWQGVFSYLKTFNPFLLLFLILASVFNLIMVVVLVRFLFAHK
                     IAILFRLLLLLFVIYMVVFTGVVGCSRYRMAIFPIIWMAFMWFLTERKSVHHKTVV"
     gene            complement(15825..20888)
                     /locus_tag="PKX59_05910"
     CDS             complement(15825..20888)
                     /locus_tag="PKX59_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOZ90438.1"
                     /translation="MRKLYVIIVLFLLPVLSFAQFSNSWINFNGASPYSAQQYFKIKV
                     WKQGIYRIDYSLLQQAGFPVGMNPNQFQIFNNGHEQYIYVEGAQDNSFDPTDFIEFYG
                     KPNNGAADQELYDDPSDQLNPDYSLFCDTAVYFLTAAILPGTPVKRMIMETDVNFSLY
                     MPENYVTKTFRRNYTSKYLKGDSQGDIDPSYNGPEGFSGNTFSAAAPADNFSLPNVST
                     FATAPIPSADVRMVNANYDGHFHTFNVEFNGNTIFTDNTIFGYGRHDYNFNIPAASLP
                     LSNSLVFSGVANSGGTNLMSVTYFKLQYPHANSFNGELEPFQFFSVSNGGSKARVDFT
                     DFLNSDNSTRFIYIIAGDTVSKVTTVRTGNLLQALIPVNGGEKNCLLAPASAYYFSTS
                     DFYLSPENSDPDPQKFARFTNYSYNQPQYDYLIVSHKKLWSEAVNYRDFRAQTGFNPL
                     LVDIDELYDQFAYGIYKHPLAIKNFCRFSITAFNNKPDYLLLLGKAVYPEEVRVSPTA
                     DQLCLVPTYGNPPSDYMFSNKIVDTTFVPRLATGRIAAQNITDVTNYLEKAQAHEAAR
                     QNCPDDWLKHVLHFIGGNNGFEQQSIKAFMDQNEAAIRDTSMGCTVTNYYKISTDPIE
                     YIQAIELQARIDSGVSIMTFFGHAAGSTFDIATDIPQNWHNAGRYPVVFAQSCNVGDI
                     YSPSRLLNEDFVLLPQKGSVGFLAKPRLGNIQELGEYSVQLYRNVAYRDYDKPVGMAI
                     KHALDSMIQTYYGNNGMVYKSTALGMLWHGDPGLRLAPSLQPDYAVSQPSITFQPTEV
                     TSELADFKMNIAISNFGKATKDSVSVHVVRKFPDNSTSVMDTLIPYIPYRDTLQLTMN
                     VDVNKGTGLNYFDVFVDYAGAITECNEFNNRVLNVPLQIISTDITPVYPFKYAIVPDP
                     SVILKATTDNIYAAQKTYRFELDTTDAFNSPFLRFKTFTQTGGIVKWPLPFTCDSGRV
                     YYWRVSLDPSLYPTTFKWKESSFIYIPQKTGWSQAHFFQFKNDSYTNIVYDKPQRKWE
                     YENTQAELRVSAWDVPGQGITSDNVQLNNQLIATGGCNYRAIYAVVLDSISLQNWSTL
                     DFAGKFGNINQPPYYDSCQATVNGSQGFFAFATDSAYSVGGNTVYRNLDSLANFINRI
                     PTGNYVVLYSVTEHFMSKSWSGQFRFALSQQGINNVDTIPDTKHFIFFFKKGFPGTAQ
                     TVVAATDSTKNITFTTMLGGNWYKGYMMSEIIGPAIQWNSLHWANNSVEAINTDTLAL
                     DIVGVDTLNSEHVLYSAIQPSQIDFILNINPFIYPRIYLRAYMEDSVYRTPPQLNRWQ
                     IYYQEVPEAVLNPVGSSFQSNSVSQGDTIHWKMPVENVSLSDMSDLLVDYYLFDKDNI
                     RRNITSTRFHPLLHGDTLNAAVKFSTAAYPGLNSLWMEVNPRNDQPEQYHFNNYARID
                     FNVNRDVTNPILDVTFDGQHILNGDIVSAKPQILIKLKDENRYLALNDTSKFKVYVKS
                     PDGVESQIYFETSANSALSEKLLRWTPAQLPDNTFKIEYNPIFNVDGIYELRAQARDE
                     SGNLSGSNDFRISFEVINKSSITNVFNYPNPFTTRTQFVFTLTGSEVPTYFKIQIYNI
                     SGKVVKEITQQQLGNIHIGRNITEYAWDGKDEFGDQLAKGVYLYRVITQLDGDDIEHR
                     NTTADKYFRKGWGKMYLLK"
     gene            complement(20885..21958)
                     /locus_tag="PKX59_05915"
     CDS             complement(20885..21958)
                     /locus_tag="PKX59_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOZ90439.1"
                     /translation="MKIVTLTWCCLILSIASVAQTAPKYSNEFLNLGVGGRALGMGGA
                     QVSQVNDVTAGYWNPAGLVHSRGDIQVSFMHNEYFAGIAKYDYLALTAPIDTMRRIGF
                     SVLRFGIDDIANTIDLKDNDGNIDYNRVTGFSAADYAFLVSYASKSAISGLDYGANFK
                     IIHRKVGDFAKAWGFGLDAGIQYQAGNWRTGAMLKDVTSTFNAWSYTLNDRTKEVFIQ
                     TGNEIPENGLELTLPRLILGVSRQFNIKKFTITPALDLETTFDGKRNTLVKTNFASID
                     PRVGIEGGYNNLVYVRAGVSNFQQVTDIDGKKTTTVQPNIGVGIKLRNLSIDYALTNI
                     GNSNYLYSNVFSLRLTVNKLKKT"
     gene            22244..>22350
                     /locus_tag="PKX59_05920"
     CDS             22244..>22350
                     /locus_tag="PKX59_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOZ90440.1"
                     /translation="MILLAAVFDFSDGFAARMLKAQSAIGKDLDSLADV"
BASE COUNT         6982 a         4518 c         3923 g         6927 t
ORIGIN      
        1 tctttttccg ttctaaacta attaaagatt ctgcctccaa tatttttttc aattttgaag
       61 caactacttc agcagagtca aacaacttaa cttttgattg aaaaaaatga tttatctcct
      121 tcttgattag tggataatgt gtacatgcaa gtaccaatcc atcaatggac ttaaatggct
      181 cgtatgagag atattcttcc aatacagcag aagaaatatt atcattataa aatccctctt
      241 caatcattga agcaagcaat ggtgtggcta aaggatgaaa aagtaattgt ggctttcgtc
      301 ttgaaaattt ttcctgataa gtacctgatg caatagttgt tttggttgca atgataccta
      361 cacttattat atcatcatca gcaataactg cctcaatcat cgggtcaatg acattgataa
      421 ccggaacact tcctttatag cgatcgcgca ataaagaata tgctgcggct gatgctgtgt
      481 tgcaggcaat gactatggct ttacattttt gttcgtgcaa caaaaattct gtaattcttt
      541 ctgcataact gccaattaac tcaaccgatt tatccccata aggaacgtga gcagtatcgc
      601 caaaataaat aagttgttct tgtggcaaca gtctgcctat tgctgcagca acagtaagtc
      661 cgccaatgcc ggaatcaaaa atacctatag gttgattaac tgtcatgccg cttccttgta
      721 ttttatttta atctgctcaa gcaaatcaag cagttcgttt aagctgtttg aagtcaccaa
      781 ttttactctg aaatcgcgaa aatgatccat ccctttaaaa tagtttgtat agtgccttct
      841 catttcaaga ataccgacaa tggtaccttt ccattccaca gactttatca gatgtgttcg
      901 gcatacttct actctttctg caataccggg tacggcaatg gcgcagttac cggcaagata
      961 ttctttaatc tgattaaata tccaagggta accaatactg gctctgccaa tcattatgcc
     1021 atcaactcca tatcggtttt tcatttccaa tgctttctgt ggtgagtcaa catcaccatt
     1081 tccgaaaaca ggaattgtca gtcgtggatt attcttcaac tctccaacaa ggccccagtc
     1141 ggcactgcct ttatacattt gtgagcgtgt tcgtgcatga acagttaatg cttttatccc
     1201 tatatcctga agccgttctg caacttcaag aatatttttt gttttatcat cccagcctaa
     1261 tcttgttttt acagtaacag gaagtttaac tgcatcaaca attgcctttg tcatactcac
     1321 cattttagga atatcctgca acaatgctgc acctgcgcct ttgcatgcta cttgctttac
     1381 cgggcatccg taattaatat caatcaaatc gggatgtgcc tgctctgcaa tgatggctgc
     1441 ctgtctcatg gattcaacat cagaaccaaa aatctgaatt ccgattggtc tttcatattc
     1501 aaaaatatcg agcttatgtg tgctctttgc tgcatgacga ataagtcctt cggagctgat
     1561 aaactctgtg tacattaaat cagcgccatg ctgtttacat acaaagcgaa aaggcgggtc
     1621 gctcacatcc tccataggag caagtaacaa cggaaactca cctaatttta ctgaaccaat
     1681 attcaccata gtcttatctg caacaattgc aaggatgctt actttcgtgc aaaattaata
     1741 aagatgataa aaggcaaatg gcaacatagt gatcctttat tgaaaatgtt actcttttgc
     1801 ggcattgtga tgacatgttt tggcttgatt tcggtgctaa atataccctt ggttaagatt
     1861 tttttcggca aaagtttacc ggaggtgatg agcggcttca gtagtggtga tacaacctac
     1921 atcaatgcca tgaagtttat tcagatattt tcacaattgg gactcatgct tgttccagcc
     1981 tggttatgtg gctggctttt ttcagacaat gtgattcagt tttttcaatt taaaaaaatt
     2041 aattttattc aaattgtggt gatcgttgtt gttacaatat gtttatcgcc tttaattaat
     2101 ttattacttg actggaacag tcatcttcag ttacctgctt cattagctgc gattgaagag
     2161 atgatgcgca acatggaaac ggaagcagaa aaactgactg aatctttttt agtaatgcat
     2221 acaaattttg atttgatcat taaccttttg atgattgcat tgcttcctgc aattggagaa
     2281 gaattattat ttcgaggcgt tttacaaaag ctcatccaac aggcaacaaa aaacaaacat
     2341 tgggctgtaa ttataactgg aattattttc agcgctattc acatgcagtt ttttgggttc
     2401 ttgcccaggt ttatgttagg tatattttta ggctatcttt tggtttggac aggctctatt
     2461 tgggcaccca tactggctca ctttattaac aatggttcgg ctgtgttgct cagttattat
     2521 gaacaaaaaa atgccatcaa tattgatgag gaaaagttag gaatcagaga aggtgaatac
     2581 tggatggttt ttgcatctgt tattataaca gtatcagcat gttacatact atatcgaagc
     2641 aagcaaaaat cctcttaagt tgttgtagta attactctat attgtcactt attacatgag
     2701 tgctgttgaa gttcccacag gtgtaagtta ttaacaattt attgttagct ttacgcttca
     2761 tttaaaaggt atttccaaat tgccgagaat tattgctttt tgcgtattgt attttgttgc
     2821 aaacgttacg ctggcgcagg atatttttat gtccaatggc gtggtgagcg gttgcagcgg
     2881 aactttctat gacagcggtg gtgactcaac caactacagt aataatgaaa atttcgttta
     2941 taccatttgc agtgatcaac ccggcaaatg tgtacagtta agctttcagt catttgcaat
     3001 tgaaaaagat tttgattttc tttttatcta taatggccct tctacttcat ctcctctttt
     3061 aggtacattt acaggaacca actctcctgg tacaataaca gcaacatcag gttgtttaac
     3121 aattagattt tcttctgatt acactgttaa cagaggtgga tggaaagcat acgttaattg
     3181 tattaattgt cctgttaatg gttgtcccac atgtaacggt ggtgctcctc cgccaaacga
     3241 tgcttgttca ggtgcacaga atcttggatt attgccacaa cctgcggcat gccccaacgg
     3301 cattggcgca gttaatctta caaatacatc taacctttgt gcaactgcag aaattccata
     3361 tcagtcgctt cagggttgta aaccaattgg cagtatgtca aatccggcta ctgatgtttg
     3421 gtacaaattc agtctgacgg cacctgtact tgatataacc atcaatggtt taataacacc
     3481 acaggttgga ttatattccg gaacaggatg cggaaattta gttccacgtg gttgcgctat
     3541 tggcggtggt ggttatttgc ataccacttt tggtggtctt gctgcaggca cctattatct
     3601 tcaggtcagt ggtggcaaca tcaatgatca atgtaatttt tctttatctc ttcaaaataa
     3661 tttcgactgt agtggctgtg taatgcaaag tggactgacg gtttcacctc caccttctaa
     3721 tggattttat ctttcagggc aacaagtaac attttgtttg aatgttaccg acttcaatcc
     3781 tacttctgca aactggcttc atggcattgt acctaaattt ggtccgggat gggacaccac
     3841 gacaataatt gcattgccac cggcatcatg ttccaacaat gggttctggt catggtactc
     3901 aaatccaata atctcatcag ccaacaacaa accttacggt ccgggatttt tttatgaaac
     3961 ttctgcaggt aatagcagca atactcaaga taataatccc ggaaataatt tcggtgataa
     4021 ccttggacag aattgtaatc tcaatttttg ctggaagata aatacactac ctcaatcatt
     4081 atgcaacttt ggcaccaacc tgaatgtttt tattgaaacc tataccgatg gagaaagtgg
     4141 ctcgtggaca tctgctgcct gtgccaacga tcctgttcac gatttatatg caacccttgc
     4201 atgttgtatt ccaccggtgg tggcaattac gcaacctgtt tgctttggta cgcaaggttc
     4261 tgcaataggt actgcacaag gttcaggccc gtggacattt atatggaaaa attcttccgg
     4321 agtaatcatt cagcaaaatg gcccggtgaa tggaattgat ttaatcagta acctgaatcc
     4381 cggaacttat caactcataa cagaagatgt taatggttgt caaagtacgg taagctttgc
     4441 agtaacacag cctcaaccac taactgccac cttagctgtt tcagcaacaa aatgtggatt
     4501 ggtaaatggc tcaataaata taacagcatc aggaggaact gcaccatata gttacagcat
     4561 taacaatggc gtttcgtttc aaggtagtcc gcaattttct aatttgaatt caggaaattt
     4621 tataattaca attaaagatg cgcataattg tacagtatca gttcctgctg ctgttaacgg
     4681 cagcacagta ccggtgatta atagtcttac atcacaaaat gtaacatgct ataatggtgc
     4741 agatggtgaa gtaacagtta cagcttcaag tggaatagca ccttatgttt atgaactaaa
     4801 aaagagcggg cttacacttt cacaaagttc aagcatcatt caaaatgttc ctgccggaac
     4861 ttatcagcta actgtaacag atgtgaacgg ctgcaaatca gacacgacag taacattgac
     4921 acaaccacca cagataaata tggtgagtgt gattacacct tcaggatgta atctcaatga
     4981 cgggacaata gaattaacta ttacaagtag tttttcagga acattacttt atagtattga
     5041 taatggaact aactatcaga caggaaactt attcagcaac cttagtccgg gagtttataa
     5101 tgtagtagtt gaagatggta acggttgtat gataaaagat acagccattg taaacactat
     5161 aaatgcacct aatattgata gtgtaaaatc acaatcccta acttgttatc gtacgcaaga
     5221 tggtgcaata aatatttttg gttcaggtgg tataggtaca ttggcttata gcatcaataa
     5281 tggctcaaca tattcggcaa caaactcctt taatacactt atagcaaaaa catatacagt
     5341 agttattaaa gacaacaata attgcagagt atatcaatta gcagcagtaa aagaacctcc
     5401 accattaata atcagaggtg atataattgc aacaagctgc ggacttaata atggttcagt
     5461 caacctacat actgacaatg gcattttacc tttagagtat tcaacagatg gtggtttgac
     5521 atggtcaaca aattactatt atggacaact tccaacagga actatcacct ttacagtacg
     5581 tgatgcaaat gggtgcacat ctacaagacc ttatcaaatt gcagcatcaa ctgcaccttc
     5641 ttttgataat gtttctgttc aatctttaaa atgtttcaac accaatacag gaagtataac
     5701 tctgaattcg attaatggta tccctccaat tacatatagt attgacagtg gtgccaccta
     5761 tcaaccaaca aatcagtttc aaaatcttat ttcaggaaat ttttatttgt caattaaaga
     5821 taataaagga tgcagagccg atactttggt acaattatca caacctattc cattgtcatt
     5881 tacctctgca gtaaccaata caatatgcag ttatccaaat ggcactattg atatcactgg
     5941 caatggagga actataccat atacctacag tattgatggt ggtgctacat ttaatggaaa
     6001 ttcaaataat ctaaatctta atcccggaag ctataaaatt gtagtacgtg atataaacaa
     6061 ctgcgaagag gccagcaccg ttagtataat tgatgaaccg ggaccaagaa taacaggaca
     6121 taattttaca ccacaaattt gtgataatgc agctaatgct acagttacaa taaatgcaat
     6181 tagtggaaca ggcacattat tttatagtgc tgacagcgga ctaacttaca gcattgcaaa
     6241 caacattaac aatctcattg gaggaagttg cacgttgatt gttcgtgatg ccaacctttg
     6301 tactgataca gttgcattca atattcctat cttacattcg ccacagatag acaacaatgc
     6361 tactaccaac ccaatatgtt ttggaagtaa tgatggagct attcagatag ttgcatcagg
     6421 tggaaatgga aatctaaatt atagtattga taacggagtt aattttttta ctaattcaac
     6481 ttttaatcaa ctcaatgcag gaacatatta tttgactgtt gaagactcaa atcactgtca
     6541 ggtaaaagat actgtcctac ttacacaacc accattactt acagcacaaa ccatagtaag
     6601 taatgaaact tgcagtaacc aaaatggcgt ggtttcaatt attgcaggtg gtggtactcc
     6661 cacatattat tttgaactaa gcaataacac aacagcaact gatacctttt ttacacagtt
     6721 aggtgcaggt aattattcaa taacaacaac tgattataat ggctgtacta cacaagctgt
     6781 ctttcagata aacaatcttg ataaaccgac aatcagtcaa actgccttaa ccaatcttag
     6841 ttgctatcaa tcagaagatg gtgttataca actagcagct ttaggaaacg gaacgatgca
     6901 atatagtctt gatggaataa tttttcaaaa ctcaaatata tttaatcaac tatcagcagg
     6961 aagctacaca attactgttc gtgatactaa tggatgtact gccgacacaa ccatattatt
     7021 gaccgaaccc aatgcaatta ctgccaacat acaaacaacc tcatcaaatt gtacactaag
     7081 caatggctct ttaacagtta atgcttcggg tggctcaggt aatttatctt attcacttaa
     7141 tggtggtacg tttaactccc aaacaatatt caataatttg tcatcgggaa atcagagtat
     7201 aactattcag gatgcaaaca attgccaggc agcatttgta gcatcaatta atacaattaa
     7261 cggtgcagtg attaataata tttctactgt tgatataaaa tgccacggcc agcaaacggg
     7321 tgaaattaca attactgctt caggaggaac cggtccacta tcatatagta ttgacaatgg
     7381 tatttcatct gtttcgagta atcactttac aatgctagca gcaggaaatt acaacatcat
     7441 tcttactgat agtgttggct gtattacttc atcaaatatt atactaaacg aacctgattc
     7501 gataataatt cagcaagcag ttacagcagc tacctgcaat cagaataatg gttcttttca
     7561 aatagtcgtt aatggtggca caggaatact tcaactttca ttagatagca tcaactttag
     7621 tccaggcaca atttttaaca accttatagc aggaatttat cagctacatg ttactgatgc
     7681 caaccaatgt tatgcaacca aattaatttc tgttccaaat caggctgcac ctgtacttac
     7741 taatcttagt aaaggtgatg ttacatgctt tggtgacagc actggtttta tttcattatc
     7801 ggctagtggc ggaacaggat tgctgaatta tagtattgac aatggtgtaa cctatcaggc
     7861 attgccgaac ttcaacaatc ttcctaccgg ttcttatcat ataaaaatct cagaccagaa
     7921 tctttgttca accgatacaa taattcaatt gacatcacct gatgatatta cagcgacatt
     7981 aactatcacc aatgctaact gccaacaaag taatggcagt ataaccgtta atgcacaagg
     8041 cggaaacgga acattattat tcagtcttaa tggcggccca ttcagtcagc agacatattt
     8101 tggttcgcta ccggcaggga cacaacagtt gcaggtgaag gatggcatga tgtgcgataa
     8161 aaatattcct gtacagataa acactatcaa tggccctaca ttagatactg taataacaca
     8221 aaatctaaat tgctttggaa ttaataatgg cacaatttca attctggcat caggaggtac
     8281 cggtgcatta ggttatagtt taaacaatca atcattttta aacactccta atttcaatca
     8341 gctatctgca ggcaactatc atgtatcagt agttgatagt gcaggatgtt tagtatcggg
     8401 acttgtacaa ttaacaacac cgtctgcaat tcaggtcaca ccaactgtta ctaatcccgc
     8461 atgcggacaa aacaatggtt cacttaattt acaattagcc ggaggcaccg gaactttact
     8521 tacttcattg aatggatctt catataacaa cattacaaat tactcgaatc tttatgcagg
     8581 aagttatacc cttaaaatta aagatgctaa taattgcgaa gtgcagcgtg cagtttcact
     8641 aagcaacatt cttgcaccaa agcttattgg atctgtatta aagcaggtta aatgttttaa
     8701 tgccaatgat ggtgaaataa aaattctggc aagtggagga acaggtgttt taacttacag
     8761 tattgacaat gggataacca cacaaatatc aaacacattt agcaacttaa ctgcaggcac
     8821 gtactccgcc tctgtaatag atcagaacaa ttgcagaagc gatacaacca ttactttatt
     8881 gcagcctgac tctttacaac tgttattaaa cattactcct gaaacatgtt cagccggtaa
     8941 tggaagtatt actgtttcat caacaggagg cacttctcca tatttatttt catcagacag
     9001 cggacaaaca ttttataatg tagcaggatt tgcacagctc gacagcaata tttattacat
     9061 aatagttaaa gattcaaaga actgtcgcac tttaaatcag gttaatatta caaacctaac
     9121 cgggcctcaa atcgtttctt taacaacaac gaatgtttca tgctatggtt tgcaggatgg
     9181 tgcagtgcaa attgcaactg caggtggaaa tggcacttta agtttttcaa caaataattt
     9241 tattacacaa cagacagaca ctttctttaa tgctttactt ccgggtaact atactgttta
     9301 tgtaagtgat actaacaatt gcaaaagcaa tcatttcttt tctataagtc agcctacggt
     9361 tgtacaaagt aacattgtct caacacaacc ttcctgtttc ggatacaata atggctcact
     9421 cattgtaaca gcaaatggtg gaacaccacc ttataatatt ttgtggtcgt caggaagcac
     9481 tgacttcaat ctttcaggta ttgtatccgg gcaatactcg tttattataa cagatgcaca
     9541 taactgcaac aaatcggaca gcatatattt atcaagtcca acagttttag ctgcaaatca
     9601 cactacacaa aacggaacat gttcaggcaa tgcaaatggc tctgcattag tgtctgtaac
     9661 aggtggcaca ccaccctatc aatacaactg gtcgccaatt gcaggaaaca ccaactacgc
     9721 caatgcccta cccgctggca tatacactgt tgatgttact gataaaaata attgtactct
     9781 gactgaacaa attcaaattt ctacacctgc accaattgca aatacttttg caaaaaatga
     9841 tgtttcatgc tttggaggaa gcaatggaat gataacagca aatgccttag ggggaactgc
     9901 accttacagc tatttgtgga gctatcagca acaaacaaat gataccatcc taaacctgcc
     9961 tgtaggcagc tattcagtga ccatcacaga tgccaacggt tgcgataatt tttttacaga
    10021 acaaatttct tcaccacaac agattaaacc taattcaata attaacaatg tttcatgtaa
    10081 tggattcaat gatgcatctg ttacactaaa cgttcaggga ggtgttgctc cttatagtta
    10141 tcaatggaac aatggagtta cacaatcatt aaatacgaat cttgcagcag gaatttatgt
    10201 agttactgtt acagatgcaa ataactgcaa cagagttaaa gcgtttacaa taactgaacc
    10261 actgccaatt ttactttcaa tttatcctgt ggacactatc tgtattggtc agcaggtatt
    10321 gttaaatgca atagcatctg gtgggacagg aacctacaat tggctttgga ataaccaagc
    10381 cgctgcatca acaatttcag tcactccatc agtaacaaca aactattttg tttctgttac
    10441 agacagcaac aactgcccat ctaaaactga tagtatttta gtaattgtat ttcctccgtt
    10501 aggaattact gcaacagggt atgatacaat ttgcgcagga cagtctgctg ttctgtcatc
    10561 aattgctaat ggtggaaatg gtggaccgta tagttatctc tggtcaaatg gaagtgatag
    10621 cgcaactgta aatgtgtcac catcggttac aacaacatat actgtaagca ttacagacaa
    10681 ctgtacgcaa atgccggcaa cagcaatggt accggttgtg gtaaacgaaa caccctcact
    10741 tgatgtttct ccgatgactg agcagggttg tatgccattg gaagtgcaat tcctcaatag
    10801 ctccacctat ccacagggga caatatttac ctggaatttt ggcgatggtc aaacaactga
    10861 ctccattaca ccattacata tttacaacgc tgacggcatt tacacagtaa gcgttcaggc
    10921 aataactcct gataattgtg taagcaatat tatcatttcg aatccaattg aagtttatcc
    10981 gttgccggtt gcctcattca acataagccc tgaaagtatt tctattttac atcctgaaat
    11041 aaatatttct gacagtagtt tgcttggctc catctggttt tatgattttg gagacggaca
    11101 aacttcaact ttgcaaaatc ctaatcacat ctatcaggat accggaaggt ttaccgtcat
    11161 tcaaattgta gaaagtgagt ttggctgtcg cgacacttca aaaagagagg ctattgttga
    11221 aggtgttttt actgtttata tcccaaatgc atttacgcca aataatgatg gcagtaacga
    11281 ttatttcttt gccaacggtt acggcattac cgaactgaca atgcagatat ttaaccgttg
    11341 gggaaataaa gtttttgaaa cgccatcagt aaccggccga tggaatggca ttgacacttc
    11401 aggtaaaatt tgtccgcaag gtgtttatgt ttatacggcc aaaatcaaag atcagtttgg
    11461 catatatcac acctacaatg gtcaggttac gttaatcaga tagacgcttg ccctaaaagc
    11521 aatacaggat tttctaagaa tccttttaca gtctgcaaaa acttagctcc tgtaacacca
    11581 tcaactacac gatgatcgca gctaagcgta acacgcatca catttcctgc aataacacta
    11641 ccatctttca caactggaac agctttaatt gcacctactg caagtatgca ggcatctggt
    11701 ggattgataa ttgctgtgaa ctcttcaata tcaaacatgc ccagattaga aattgtaaaa
    11761 gtatttcctt cccagtctgc aggttgtagt tttttatcct ttgctttctg cgcataagtt
    11821 ttcacttcgg cattaatctg tgaaagtgtt tttgaatctg caaaacgtac aacaggcaca
    11881 agtaagcctt catcaactgc cactgcaaca ccaatatgga tgtgatgatt ggttctgata
    11941 gtatcgccca accaagatga gtttacttta ggatgctgtt gcaatgcagc ggcacatgct
    12001 ttaatcacaa aatcattgaa tgaaactttg cttcctgtgg ctgcattaat agcctcgcgc
    12061 gcatccatac atcttgacat ttcgatatct aatgtaagat aaaaatgtgg tgcagtaaat
    12121 ttactttcgc ttaatcttcg tgcaatggtt ttacgcattt gcgaaacagg tgtttcttca
    12181 aaagactcct gtgtgcgtat tgccgaagta taacttcctg caacaacatt gccttgttta
    12241 aaccattcca catcacgttt cacaatcctg ccattatctc cactaccttt tatcattgtt
    12301 agatcaagac ctttctgccc tgcaagtgat cgtgctaatg gtgatgcttt tactctacta
    12361 tcagattctg atgctactac tggttgagta acaggttttg cttctacttt tggtgccgca
    12421 acagttttta ccggagtttc agcaacaacc ttcgctgcag gttttgcctc agtcttttcc
    12481 ggcacagcag ctgttgccgt ttttgctttt gactcagctt caagcaatgg cttaaaatct
    12541 tcacccggct tacctaatat tgctaatata ctatcaacag gcgcagcttt tccttcttca
    12601 acaccaatat aaagtaatgt tccgttttga taactttcaa actccatagt ggctttatcg
    12661 gtttcaatct ctgcaaccaa ttcgccagac tttatctggt caccaatttt tttatgccat
    12721 ttagcaacaa cgccttctgt catggtgtcg ctcattttgg gcattctaac tatttctgcc
    12781 atgtttgatt ttacttgtaa tgcttgttaa ttaatcttca ataatgtaag gataatcgga
    12841 ttgctcatag acatctttat acagttcatc tacatcagga aatggtgatt cttctgcaaa
    12901 ggtcacactg tcttcaacaa tatcgtgaac ttttttgtca atcgcatcta tctcttcctg
    12961 tgttgcaagt ttatttttct gtattacttt cagtacttct tctatgggat cttgtgcttt
    13021 atatttttcc agcttttcct tacttctgta ttttgcagga tcactcatag agtgtccttt
    13081 ataacgatac gtattcatct ctaacaaata cggaccattt ccttttctgc aatgacttac
    13141 tgctttatcc atagcctcat gcacagtttc taccatcata ccatcaacag gttcgctggg
    13201 catattaaat gcagtaccta attcatataa atcggtaaca ttactggttc gttctacaga
    13261 agtacccata gcataattat tattctcgat aataaaaact accggtagtt tccatgtcat
    13321 tgccatattc aatgcttcat gaaatgcacc ttgacgcact gcaccatcac ccatataaca
    13381 taaagtcaca ctatctgtac ctttatattt ttcggcaaat gcaatgccag cacctaatgg
    13441 aatttgtccg ccaacaatac cgtgtccgcc aaaaaaacga tttgcagcat caaacaagtg
    13501 catacttcca cccttacctt tactgcatcc ggtagcttta gcaaacagct ctgccatgac
    13561 tgctcttgcc gaagttcctt ttcctaaagc atgtgcatga tcacgatatg ctgtaatcat
    13621 attgtcggta ggtttgataa ctgacattgc ccccgcaaca acagcttcct gtccaatata
    13681 caaatgacaa aatcctttta tcttttgcat accatataac tgtcctgaac gttcttcgaa
    13741 cctgcgcatg agcaacattt gttcatacca aaaaagatag gtttcctttg taaatttctg
    13801 tttagccata ttctttataa tgctacaaaa atagttaaat actgtttgcc tatttccctg
    13861 atagttgatc tgaattaaac gaaaaaggca atagggcttt tatactgtcg agtacaatta
    13921 ttttgttctc agaaccatac atgataaatt taatgtcctg atgataaagg tgttcatact
    13981 ctgccattac ctgacggcat gctccacaag ggcttgccat cacaggctct tcctgacttc
    14041 cgggcatagc tgttacagca attgctataa ttttaacacc gggatactga acagctgatg
    14101 caaaaacagc cactctctct gcacacaggc cggaaggata tgcagcattt tcctgatttg
    14161 agcctttaat tatttctcca tttgctaaca acaccgctgc tcctacgtga aaatcagagt
    14221 aaggcgcata agctgtctct gccgcttcat gtgcttccaa catcagactt ttataatctg
    14281 ccggcaattc gtttaacgaa ttgtattcct cgtaactgct tgttaactga attttctgca
    14341 tcataaatat tttctaatca gatggctaaa ttaagcaatc gctgaaatta aaagctgtca
    14401 ttttattggt ttgaaagtaa cctaaaaaat caggtctaaa aaaatcatca tcttggtttt
    14461 aaactactgt tttatgatgt acagatttcc tctctgtaag aaaccacata aatgccatcc
    14521 agattattgg aaatatagcc attcgatagc gactgcatcc aacaactccg gtgaacacta
    14581 ccatataaat tacaaacagc aatagaagta aacggaacag aatagctatt ttatgtgcaa
    14641 ataaaaatct gactaatact accaccataa tcaggttaaa gacactagcc aaaatcagaa
    14701 acagcaataa aaaaggatta aatgttttaa gataactgaa cacaccttgc catccttgct
    14761 cctcatactt ctgcttccac ccttctgttt gcacataatc cggattcaca aaaaatgcca
    14821 taaggtcaaa cctgccatga tcaataaaaa agttagccat accctttaat tgcatcatca
    14881 caaaacgtgc cttattctct ttaatcagaa acaaactttc tttattaata tacttacaat
    14941 aacttttaaa atcaggttgc ttattggctt cactaagcaa agaatcaatc ttttgctgtg
    15001 cacttatatc tcctttatct cccactgcca gataggcacc atattcagag ataaactttt
    15061 catttacgct gctgtaatga aaatacccgg tcttggaata gttaattgtt gaaattgtgg
    15121 ctacagtcaa taatggaatc agtgatatat aaacattgct caacttataa atgccctttg
    15181 tataaaacaa cagtgctatg actggaacta tcaaacaaaa ggcataaaaa accggctttg
    15241 ttaaaatagc caacgatata aaaatacaga aatacaaaaa tgaatcaggc cttcttaccg
    15301 attgaataaa gaacaaaaat ccgctgataa tcaaaaactg aaacaagaca tcagccatta
    15361 tcatgtttgc ataaataaat tgtgtcggaa acagcaataa gggtaatatt accaaccatt
    15421 gattggcaat ccgctttccg gtcagcagcc aaaacataaa ggctgtaagt ataagcactg
    15481 caatgctcat taaattctgc aatgccagta aaaagaaatt atttgatgaa actatatgca
    15541 ctaaaaaaat aaagaagcca tataacggag gccttcggct ttctaaaaag tcatcgtgtg
    15601 gtgttgatga actaccagca taccatgaag catgttcgcg tatgttttct gcctgattta
    15661 aatattctgt actgtcaaca gaaaaggcta tgttgccatt tcgcaaagcc agaagaaaaa
    15721 atatgagatg aatcacagcc aaagcccaaa gaagaaatgt gttcttcttc aaccataatg
    15781 aaaaataacc ggcaagggct ttcataggtc tttaaaaggt tgccttactt taagagatac
    15841 atttttcccc atccctttct aaagtatttg tcagcagtgg tattccgatg ctcaatatca
    15901 tcaccatcaa gttgtgttat tactctgtaa agataaacac cttttgccaa ctgatcacca
    15961 aattcgtctt ttccatccca tgcatattct gtaatgtttc gaccaatgtg aatgtttccc
    16021 aattgctgtt gtgttatttc tttcaccact ttgccactga tattatagat ctgaatttta
    16081 aaataggtag gcacttccga tccagtgagt gtaaacacaa actgtgttcg tgttgtaaat
    16141 ggatttggat agttgaacac attggtaatg ctactcttat taattacctc aaaagaaatt
    16201 cgaaaatcgt tgctgccgct aaggttacca ctttcatctc ttgcctgagc acgaagttcg
    16261 taaatgccgt caacgttgaa aatcggattg tactctatct taaatgtatt atcaggaagt
    16321 tgcgcaggtg tccaacgtag taatttctct gacaatgctg aattcgctga tgtctcaaaa
    16381 taaatctgac tctctacacc atcaggactt tttacataca ctttaaattt tgaagtgtca
    16441 tttaaagcca aatacctgtt ttcatctttt agtttaatga gtatttgcgg ctttgcagaa
    16501 acaatgtcgc cattaagaat atgttgtcca tcaaaggtaa catcgagaat tggatttgtc
    16561 acatcacggt ttacattaaa atctattctt gcataattat tgaaatgata ttgctccggt
    16621 tgatcatttc gcggatttac ctccatccac aatgagttta atcccggata agctgctgtg
    16681 ctgaacttta ccgcagcatt caatgtgtcg ccatgtaaca aaggatgaaa gcgtgtagaa
    16741 gttatatttc gtcttatatt atctttatca aacagatagt aatccacaag cagatcactc
    16801 atatcagaaa gacttacatt ctctacaggc attttccaat gaatggtatc gccttgtgaa
    16861 acactatttg attgaaaaga agaacctaca ggattcaaca cagcttccgg aacttcctga
    16921 taatagattt gccaacgatt gagttgcggt ggtgtacgat aaacagaatc ttccatgtat
    16981 gcacgcaaat aaattcgcgg ataaataaac ggattaatat tcagaataaa atctatctgc
    17041 gatggctgaa tagcgctgta caaaacatgt tctgaattta atgtatctac accaacaata
    17101 tcaagtgcca gcgtatctgt attgatagct tccacactgt tgtttgccca atgcaaactg
    17161 ttccattgaa tggcaggacc aataatctct gacatcatat agcctttata ccaatttcca
    17221 ccgagcattg ttgtgaatgt aatattcttt gtactgtctg tagctgcaac aactgtttgt
    17281 gccgtacccg gaaatccttt tttgaaaaag aaaataaaat gtttagtatc aggaattgta
    17341 tctacgttat taattccctg ttgcgataat gcaaagcgaa actgccctga ccatgacttg
    17401 ctcataaaat gctcagtcac gctgtataat accacatagt ttcctgttgg aatacgattg
    17461 ataaaattcg caaggctgtc gagattgcga tatacagtgt tgccaccaac gctatatgca
    17521 gagtctgttg caaaagcaaa aaatccctga ctgccattta ctgttgcctg acaagaatcg
    17581 taataaggag gttgattgat gttaccaaat tttcctgcaa aatcaagtgt actccagttt
    17641 tgtaaagaaa tagaatcaag taccactgcg taaatagcac gataattaca gccacctgtt
    17701 gcaatcaact gattattgag ttgcacattg tcagaggtaa ttccttgtcc gggcacatcc
    17761 catgcagata cacgcaactc tgcttgtgtg ttttcatatt cccacttacg ttgtggttta
    17821 tcataaacaa tgttggtgta gctgtcattt ttaaattgaa aaaaatgtgc ctgactccaa
    17881 cctgttttct gtggaatata aataaaagaa ctttctttcc atttaaatgt tgttggataa
    17941 agtgatgggt caaggcttac acgccaataa taaactctgc cactgtcgca tgtaaaaggt
    18001 aatggccatt taacaatgcc tcctgtttgt gtaaacgttt tgaatcttag aaaaggtgaa
    18061 ttaaatgcat cggttgtatc taattcaaaa cgataagttt tttgtgcagc ataaatatta
    18121 tctgttgttg ctttcaagat taccgaaggg tcaggaacaa tagcatactt aaatggatac
    18181 acaggagtga tatcagtaga aattatttgc aaaggcacat taagcactct gttattaaat
    18241 tcattacact cggtaatagc acctgcatag tcaacaaaca catcaaaata attcagccct
    18301 gttcctttat tcacgtcaac attcatagtc agttgcaaag tgtctctgta tggtatataa
    18361 ggaatcaatg tatccataac tgatgtgctg ttatcaggaa attttcgtac cacatgcact
    18421 gaaacagaat ctttcgtggc tttaccaaaa ttacttattg caatattcat tttgaaatcg
    18481 gcaagttcag atgttacttc tgtaggctga aatgttattg atggctgcga aactgcatag
    18541 tcgggttgca atgaaggtgc taatctcaat ccgggatcac catgccacag catacccaag
    18601 gcagtagatt tataaaccat tccattattt ccataataag tctgtatcat agaatccaat
    18661 gcatgtttta ttgccatgcc tacgggttta tcataatctc tgtatgccac attacggtat
    18721 agttgtacag aatattctcc caactcctga atatttccca gtcggggttt tgctaaaaat
    18781 cctactgatc ctttttgtgg aagcagaaca aaatcttcgt tcagcaatct tgaaggcgaa
    18841 taaatatcgc ccacattaca agattgtgca aaaactacag ggtagcgtcc ggcattatgc
    18901 cagttctgtg gaatatcggt tgcaatgtca aaagttgacc ctgcggcatg tccaaaaaat
    18961 gtcataatgc tgacaccact gtcaatacgt gcttgtaatt ctattgcctg aatatactct
    19021 attgggtcag tagatatttt ataataattt gtaaccgtac aacccattga tgtatctctg
    19081 atggctgcct cgttctgatc cataaaagct ttgatgctct gttgttcgaa gccattgttt
    19141 cctccaataa aatgcaaaac atgtttcaac caatcatcag gacagttttg tcttgcagct
    19201 tcatgtgcct gggctttctc aaggtagttg gtaacatcag ttatattctg tgcagctatt
    19261 cggcctgttg caagccgggg cacaaaggtt gtgtcaacaa ttttattgct gaacatatag
    19321 tctgaaggcg gattaccata ggtaggcacc aaacaaagtt gatcggctgt tggacttact
    19381 ctaacctctt caggatatac tgccttacct aacaacaata aataatcagg tttattatta
    19441 aatgcagtga tagaaaaacg gcagaagttt tttattgcta acggatgctt gtaaattcca
    19501 taagcaaact gatcgtacaa ttcatcaata tcaacaagca aaggattaaa tcctgtctgt
    19561 gctctgaagt cacgataatt cactgcttcg ctccatagtt ttttatgcga gacaattaaa
    19621 taatcgtact gcggctgatt gtaactgtag ttggtaaaac gtgcaaactt ctgtggatcg
    19681 ggatcactat tttcgggaga caaataaaaa tcggaagtag aaaaatagta tgctgatgcc
    19741 ggagcaagca ggcaattctt ttctccacca ttaacgggaa ttaaagcctg aagtaaattt
    19801 cctgttctaa ctgttgttac tttagaaaca gtatcacctg caattatata aatgaaacgt
    19861 gttgaattgt cgctgttcag aaaatctgtg aaatctactc ttgctttact accaccattg
    19921 ctgacggaga aaaactgaaa aggttccaat tctccgttaa aagaatttgc atgaggatac
    19981 tgaagtttaa aataggtaac agacataagg tttgttccac ctgaatttgc tacgccacta
    20041 aacaccaaac tgtttgaaag tggtaatgat gctgcaggta tgttaaaatt atagtcatgt
    20101 cgcccgtagc caaaaattgt attatcagta aaaatagtgt tgccattgaa ctcaacatta
    20161 aatgtatgaa aatggccatc atagttggca ttaaccattc taacatccgc actggggatt
    20221 ggcgcagtag caaatgtaga aacattggga agtgaaaaat tatctgctgg tgcagctgca
    20281 gaaaaggtgt tacccgaaaa tccttcagga ccattgtatg atggatcaat atcaccctgc
    20341 gaatcacctt tcagatattt tgaggtatag tttcttctga aagttttggt aacataattt
    20401 tccggcatgt aaagagagaa atttacatca gtctccataa tcattcgttt caccggtgtg
    20461 ccgggtaata tagctgctgt aagaaagtaa actgccgtat cacaaaacaa actatagtcg
    20521 ggattcaact ggtcagacgg atcatcatac aactcctgat cggctgcacc attatttggc
    20581 ttaccataaa actcaataaa atctgttggg tcgaatgaat tatcctgagc accttcaaca
    20641 taaatatatt gctcatgacc gttattgaaa atctgaaact ggttagggtt cattccaaca
    20701 ggaaaacctg cctgctgcaa caagctgtaa tctatccggt aaataccctg cttccaaact
    20761 ttaattttaa aatactgttg tgcagaataa ggtgatgctc cgttaaaatt aatccaactg
    20821 ttcgaaaact gagcgaaact taaaacagga agaagaaata aaacaattat tacgtaaagt
    20881 tttctcatgt ctttttcagt ttgttgaccg tcaatcgcaa tgaaaataca tttgaataaa
    20941 gataattact atttccaatg ttagttagtg cataatcaat agacagatta cgcaacttta
    21001 ttccaacacc aatattcggc tgaacagttg ttgtcttctt tccatcaatg tcagttacct
    21061 gctgaaagtt actcacgcct gcacgcacat aaacaagatt attataaccg ccttcaatac
    21121 caactcttgg gtctatgctt gcaaaatttg tcttcaccaa tgtatttctt ttaccatcga
    21181 aggttgtctc taagtctaat gcaggtgtga tggtaaactt cttaatatta aactgtcgtg
    21241 aaacaccaag aattaatcgt ggtaatgtca gttccaaacc attttccgga atttcattgc
    21301 ctgtttgaat aaacacttct ttggtacgat cgttcaaagt atagctccat gcattaaagg
    21361 ttgaagtaac atctttcaac attgcacctg tgcgccaatt gcctgcctga tattgaatgc
    21421 ctgcatcaag tccaaaaccc caggcctttg cgaagtcgcc aactttgcga tgtataattt
    21481 taaaattagc tccgtaatct aatccgctaa tggcactctt actggcataa gaaaccagaa
    21541 atgcatagtc ggcagcagaa aatcctgtaa cacgattgta gtcaatattt ccatcgttat
    21601 ctttcagatc aatggtgttg gcaatatcgt caatgccaaa acgaagtaca gagaatccaa
    21661 tgcgtctcat agtgtcaatt ggtgctgtca acgccaagta atcgtacttg gcaatgcctg
    21721 caaagtattc attatgcata aaagatacct gaatgtcgcc acgagaatgc accaatcctg
    21781 caggattcca atagcctgct gttacatcat ttacctgcga aacctgcgca ccgcccatgc
    21841 ccaatgccct gcctccaacg ccaagattta aaaattcatt actgtactta ggggcagttt
    21901 gcgctacgga agcaatactt aaaatcaaac aacaccatgt gagggttact attttcataa
    21961 actgatgatt tggataccta taccaaacga taaatgacta attttatttt tttctttgcc
    22021 aaacatgtct gtatttaaaa atccttttaa tatttacacc aaatttatag taatccatcc
    22081 aataaaaagc atgtcaggat gtaattttct aacatttaaa tcataacaaa aaaccgatgg
    22141 cattgaagaa tatgatcccc aacttttttc cctgcctgaa cgtactgtgt ggttgtattg
    22201 gtttgtttta tgttttacag gccatataac aggaggcagt tggatgattt tgcttgcagc
    22261 ggtttttgat ttttcagatg gttttgctgc acgcatgtta aaggcgcagt cagcaatcgg
    22321 aaaagacctt gactctttgg cagatgttat
//
LOCUS       DAOOOI010000034        22263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_439_length_22263_cov_41.240904, whole genome shotgun sequence.
ACCESSION   DAOOOI010000034 DAOOOI010000000
VERSION     DAOOOI010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 22263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22263
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_439_length_22263_cov_41.240904"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2382
                     /locus_tag="PKX59_05925"
     CDS             <1..2382
                     /locus_tag="PKX59_05925"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90441.1"
                     /translation="VRGATVILRQDAVVNPTTVGAGGLGVSAADGGNGGNSSFGGSVT
                     ANGGKGGKVGYFWAQYGAGGASTAGTHSGGAGAAGASGGSGGGGGGAGSTGNGGNASG
                     TTGGTGGTGGGGTGANGRTSNGDGSDATALSAGGGGGRSNASNTDRIGGDGFRGQIKV
                     TYTCVSYSLTATSGTTPICASAGTSVISLTGTAATLPVGDYVVTYDRSLPSASNLTVS
                     MAVVTAGTGSFTATGLTSGGNSTITVTKLTSSYCNNNITLNNTTVIAVAASIPPQPDV
                     IAGAPVDVCPPTASYTLSTNSTGADSYNWHLGVGVTGVTFLSANGNNTMDVQFGATAN
                     STYVIRVDATNACGTSAYRPVMIRRATSVPAAITGLKTACASTSDTYTATAVAGATSY
                     EWSISGDATVTGTTNTATVNFGPTWTGGTLCVASKVGCFTSATKCMTISTSATVLSTV
                     LGGTFKACPNSSLNFNITASPGVATYNWTVPANASINSGQGTNTITVDFGPSYNNTGN
                     ICMTATSICGVVAPTKCKSVSPGLPSVPASITGGTNGLCNQTMPFSTPTMAGVTSYNW
                     TAPGTVNNNGNNAVTIDFGALTTGQVCVSATNSCGTSNTRCTAVKGAPNTPASITATP
                     STWCPNTAGVEFNANIANVTGAYTLSWTYPAASANYISGGGNSSSLILDWTTGNGAVM
                     ITATNACGSGTKVFDVVLDNCREAGIETVKQMDVYPNPANDLVNISYQSAATELIKVS
                     LMDYSGKLVVNQNVQVNSGMNNYSLDVSKLAPGVYMLNVQSENGNMQRKLIKQ"
     gene            complement(2472..>3351)
                     /locus_tag="PKX59_05930"
     CDS             complement(2472..>3351)
                     /locus_tag="PKX59_05930"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOZ90442.1"
                     /translation="VTIDSNSGFCFGVVYAIQQAEEELEVNGHLYCLGDIVHNNMEVD
                     RLKGKGLEIINHEQLQQLHDCKVLIRAHGEPPSTYQTAIKNNIELIDASCPVVLKLQN
                     RVRGSYDEITDTGNGQVVIYGEKGHAEVNGLVGQTGGKAIVVKTEEDLDQIDFNKPIH
                     FFSQTTKSTLGFEQMQKTIQQRIVKQKGSLEETDFITNDTLCRQVSNREPQMRRFSSQ
                     HDVVVFVSGKKSSNGKALYSVCKATNDKSYFISSAEELDATWFQGINSVGICGATSTP
                     LWLMKDVKNAIEKMTG"
     assembly_gap    3352..3361
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3426..4190)
                     /locus_tag="PKX59_05935"
     CDS             complement(3426..4190)
                     /locus_tag="PKX59_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ90443.1"
                     /translation="MKITFLGTGTSQGVPVIACNCNVCQSTDLRDKRLRTSIHIQHKN
                     SSFVIDSGPDFRQQMLRAQIKNLTALIFTHEHKDHVAGMDDIRAFNYVLQKKIDIYAT
                     LRVQEALVREFPYVFHDFKYPGVPEVNMITIDEHPFNIEGLEFIPIEVLHYKLPVNAY
                     RVGDFTYITDANFISEKEKEKIKGSRIVVINALRREKHVSHFNLEEALELIKELKPEK
                     AYLTHISHQLGKHSDVEKELPPNVFLAYDGFEIEMS"
     gene            complement(4187..4516)
                     /locus_tag="PKX59_05940"
     CDS             complement(4187..4516)
                     /locus_tag="PKX59_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90444.1"
                     /translation="MALRTSKNDMAKFTPQYLKKLEEHLLENGYDVRYEKGNFKNGYC
                     LLESKKLVMVNKFSAMETRCSALLEIINLLESQQLLSNKRQELFAAMENPEPQVVTIP
                     HSSESEH"
     gene            4661..5743
                     /locus_tag="PKX59_05945"
     CDS             4661..5743
                     /locus_tag="PKX59_05945"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HOZ90445.1"
                     /translation="MILGVPKELKNRERRVALTPDVVKSLVASGYNVVVEEGAGEASF
                     YTNDQYKNAGATLGDKAQVYGSDVVLKVNAPQPAEIAQMKKGATLISFMWAATNADLV
                     EACKQAGINAFSMDAIPRISRAQKMDALSSQANLAGYKAVVLCADTLGKILPMMTTAA
                     GTIRPSKVVIFGAGVAGLQAIATAKRLGAVVEVSDIRPETKEQVQSLGGKFIEVKGDD
                     SIKIEGGYVKGVSEEFLKRQQELVAKHIKEADIVITTALIPGKKAPLLVTEEMVKSMK
                     NGSVILDMAVEQGGNVTGSELNQTVQKNGVTIIGESNIPSLLPMNASDLYAKNIQTFL
                     LHLTTKDGFKWEMEEEITKGSLIVKS"
     gene            5987..6292
                     /locus_tag="PKX59_05950"
     CDS             5987..6292
                     /locus_tag="PKX59_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOZ90446.1"
                     /translation="MTEIINFIGTHQEIIFFIILSVFVGIEVIGGVPTVLHTPLMSGA
                     NAIHGVVIVGAIIVMLNVDANDYVALALGFLAVVLGTLNVVGGFVVTDRMLEMFKKK"
     gene            6324..7760
                     /locus_tag="PKX59_05955"
     CDS             6324..7760
                     /locus_tag="PKX59_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOZ90447.1"
                     /translation="MTPEVLYNIRTITYIIASATFIIGLKRMGNAKTARNGNLIAAVG
                     MTLAIIASIFIHVNKTQTATGVTFEQVETPGFIYTLIFAAIIIGTIIGWLTAKKVQMT
                     KMPELVSMFNGMGGACAALIGLMEFHHNTGNTGALIAIVLGLIIGSVSFSGSVIAFLK
                     LNGTMNKPVRLPSYNIINTLVMIGVFAFGAYMIFSGTTSATLLYLLFAAALIYGILFT
                     IPIGGADMPVVISLLNSFTGLAAAFGGFLYDNKVMLTGGILVGSAGTLLTLVMCKAMN
                     RPLSNVIFGAFGGGGASAGDGPDVKGSIKDMSVADAAVLLNYSKKVVIVPGYGLAVAQ
                     AQHIIHELEMLLEERGVEVKYAIHPVAGRMPGHMNVLLAESNVDYGKLVEMEEINPEL
                     EQTDVVLVVGANDVVNPAAKTDPSSPIYGMPILEVDKAKHIIVNKRSMNAGYAGIDNL
                     LFYDPKTSMLFGDAKKALTELVAELKTM"
     gene            complement(7788..9935)
                     /locus_tag="PKX59_05960"
     CDS             complement(7788..9935)
                     /locus_tag="PKX59_05960"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOZ90448.1"
                     /translation="MLSKVKLIKVFLRLSAFICLFFSSDFAVANKVDTLSFFGSKSPK
                     RDTVFSINALSNFEWNVEQYNKNGLTKKYYTDQVDTIQIQNKIPEGALLTTVFYVDSS
                     MLNKVQALFYNTNGSVKISINNEVVALCGVFDGNKHYTDVKSEQKDYKYFILKKNKNV
                     LQVQYLPALKRHNLNIRLGDAKWAAGNLKDKKDGEIRSYAVATFYFSFAIVLILFYFF
                     IKNQEYLFFGLYCLATFLQYLSEVLTQTPTIKVLSAYSFLISLELLAIFMALAINKIH
                     KTKIPLFLMIAVLIFSLIFPDDFIVTIKFVKNIEIPVIRFLSYVIFLSYSLFSFAYHW
                     IQGFGHKNWDAKLLTYGTGIGILLFLLPIGYAMNKSFDQQNISDNILNTLFTISAGVY
                     PITVAIVLARRYGKNQTQLIEQVQTIKKLGEENLLQETEKKNILAQQNILLENKVTER
                     TFELEEKNKEILNKNKEITDSLIYAKRIQAAVLPNLNTVYKILDQSFILYLPKDIVSG
                     DFYEFTQKENKHIIAAADCTGHGVAGAFMSIIGSSLIKSIINEKNITAPSGILDALNE
                     GIINSLKQHESETHDGMDISICSFDTKNMSLEFAGANRPLWLIRNDELIVYAADKFPI
                     GGLQIKHNNQFTNHQIKIQPKDTIYLFSDGYADQFGGEHGKKLMTKKLKEILVSIQHL
                     SMNEQKIYLQNYFNEWKGNNEQVDDVLIIGIRI"
     gene            10154..11533
                     /locus_tag="PKX59_05965"
     CDS             10154..11533
                     /locus_tag="PKX59_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HOZ90449.1"
                     /translation="MITVIIPTLNEEENIASVIKFAKQNSLVSEVLVVDDKSIDNTVR
                     IAQDNDARVITSTKIGKGASMREGVMFSKNEIIVFLDADINPYPHFTIKLLTDPIIHS
                     GAGFVKSSFNRNAGRVTELVAKPMLSIFFPDLLRFNQPLSGMIASKKSLLQQLDFRDD
                     YGVDIGLLIDMHLIHVNISEVEIGYIENKSKPWQALGKMSKEVAQTIIIKASSSGNPH
                     YNFEEFGVLSEIRSQMEFALNKQQETFNKLLIFDMDNTLLRGRFIDTCAERYGFKKQL
                     MDIRSSETDSTIITKRIAKLLKGRTIDELIAIADGISIVDDTEKIITKLKKRGYVVGI
                     ISDSYDCITNHVRNKLRMDFSLSNELEFSKSVCTGEVKIPSLFVNNESSSCKHVLCKT
                     NALQKLLEKYDIQKENSIAIGDSMNDLCMIKEAGLGIAFCSKDELLNHHADVIIRKPD
                     FSALLNIAK"
     gene            complement(11622..12218)
                     /locus_tag="PKX59_05970"
     CDS             complement(11622..12218)
                     /locus_tag="PKX59_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90450.1"
                     /translation="MKTFLLASALFLSTSTFAQDKPELGNNIFSLTPMAIIGNESMDN
                     NTDVAVGLNYERILNEGLVSIKIPVNISLRNSGFFYIMPTIKLYPTRQGVVRYAVGPQ
                     FYIASGQINHHDYNTVWNGNQYITVNNKYSDNHTQLGFMINNSLNVSIAKTLYIGMEL
                     GLGIKYYDSNEENEYENSFDTTPVEPNVQFSFAMGYRF"
     gene            12357..14783
                     /locus_tag="PKX59_05975"
     CDS             12357..14783
                     /locus_tag="PKX59_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOZ90451.1"
                     /translation="MSDQTQIMQKKELSFEDFRQVVLNDYRLGRISRETSLLGRREVL
                     TGKAKFGIFGDGKEVAQIAMAKVFQNGDFRSGYYRDQTFMMAAGLLTVQEFFAQLYAH
                     PSVMAEPATAGRAMNGHYATRLLNDDGSWKNQTQSKNSSADISPTAGQMPRLLGLALA
                     SKFYRNNPELKSDKFKNFSVDGNEVAFGTIGDASTSEGLFFETINAAGVLQVPMVISI
                     WDDGYGISVPNKYQTTKQSISEALQGFARTQEKPGYEIIRAKGWDYAELCDAYEQAAN
                     IARTEHVPVMVHIQQMTQPQGHSTSGSHERYKSKERLLWEEEFDCLRKMREFMVQSAI
                     ATEQELDAIDAEAKKFVNDGKKQAWDSFTSEINTEKQQALSLAEAAISKSSNGVFLNK
                     AVEEIKNITNCERRDIAVFIQQVLFTLRADNSAEIEALKQWYRQWHQLNFDRYSSQLY
                     AEGNGSALGIAEVKAVYSAEANMVDGREVLLANHDKLFSQYPEYIAFGEDVGTIGGVN
                     QTFAGMQAKYGEHRVFDVGIREATIAGQGIGMAVRGLRPLAEIQYLDYLAYAIQILSD
                     DLACLRYRTKAGQKAPLIVSTRGHRLEGIWHSGSPMQFILGALRGMLVLVPRNMTQAA
                     GFYNLLLQCDDPALVIEPLNGYRLKEKLPENLGAFTVPVGVPEILEEGNDVTLVTYGS
                     CVRVAQDAMKQLQQCGISVELIDVQSLIPFDIHHIIAESIQKTNKVVFFDEDVPGGAT
                     AYMMQQVIENQKAFEFLETPPVTLAAKEHRPAYGSDGDYFSKPNAEDVFNTVYELMHS
                     VNPQAFPLFR"
     gene            complement(14804..15370)
                     /gene="ruvC"
                     /locus_tag="PKX59_05980"
     CDS             complement(14804..15370)
                     /gene="ruvC"
                     /locus_tag="PKX59_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOZ90452.1"
                     /translation="MSESEKIILGIDPGTNVMGYGVLLISGTEMKLETAGIIHLEKFD
                     NHPLKLKHIFEKTLRLIESYLPDEMAIEAPFFGKNVQSMLKLGRAQGVALAAALFKNI
                     PVFEYSPKKIKQSVTGNGSATKEQVAAMLQQLLHFEEMPKYLDATDGLAAAVCHHFQK
                     NTGQSQFAGGKSKGWQKFISDNPDKLIG"
     gene            15451..16395
                     /locus_tag="PKX59_05985"
     CDS             15451..16395
                     /locus_tag="PKX59_05985"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOZ90453.1"
                     /translation="MLQFFLKATIALFSLWFIVHKIIHRENEVSFFDFVNTRLHTEGA
                     YSLLISVIVLMVLNWSIEAIKWKVLINRLTKISFVRSLSAVFAGATVSFFTPNRVGDY
                     AGRMLFLPSSVRIASLLSTFAGNISQLIVTLCCGLAAMALSLQHYVQWNESLVVTTRV
                     ASFVFALILFVSFPYIRIVFKMPLLNFVRQRFPEYVMHLENYSYREMMHILFLSLLRY
                     SVFAFQYILLFLVSGIPVIPSMIVIIALIFFIQALVPTLALTEFAIRGSVALMVAAPF
                     IQNDQAVLMASFALWLINLAVPAAIGSVMIFYLNFRKE"
     gene            complement(16402..17274)
                     /locus_tag="PKX59_05990"
     CDS             complement(16402..17274)
                     /locus_tag="PKX59_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90454.1"
                     /translation="MRFIISFVILLTHYISGYSQERRGPFVKTDLFTLDRDRVIDGGI
                     FTYRIAVDADEIIAFNSCNQPYVFLINNQGTILEEIKLPYEGCLRNMEFDEFDNLMMM
                     DNEETRIFKYVRKTKKIEVIPYNKPEDWYKSLNHFYRFFEIASIPTYYNNPEYYQDFY
                     KTRFHYSYNLWLNYQDGYIYQFAYNFIKKVGNRKTYLALKRSDLWFSDRLTNKCKPLL
                     VDLEKETAVYFDRSLTLYFEDFKNHTLQTWPLAEGHDEAVQLDYATNIKQKKVWGVST
                     FDNKHLTIAVWEIK"
     gene            17364..18578
                     /locus_tag="PKX59_05995"
     CDS             17364..18578
                     /locus_tag="PKX59_05995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ90455.1"
                     /translation="MLTFAQLMNTVVAIIFWISLLGLLHSYVLFPLLMRLLLMLKHKS
                     KDDDDNNLFKPMVSVVMAAYNEEKVIIQKLESVIKSNYPSDKLEIIVGSDCSNDSTDM
                     LVKEFAHNHPQVKFIRFNSRTGKPAIINQLVKQCKGEFLIITDANVMFDAEAISSMMH
                     RFTVAEVGLVDSNMVNIGLMKSGISSQEKQYIQTEVLTKYAEGEVWGSMMGPFGGCYA
                     IRKSLFSDIPSNFLVDDFYVCMKVLEQGYHSVSSLKAIVYEEASHNLWIEFRRKTRIA
                     TGNFQNLAKFSKMIFKGRGIGFCFMSHKVLRWKGPVLMLLLYFSSIAAWWLSIDINPV
                     LYLGVFAAISLSLLLLLADRLLNLFQIHIGLLRLNTHFLVMNAAMLKGMFRYIKGVKS
                     GVWQPTSRTQNA"
     gene            18631..19020
                     /locus_tag="PKX59_06000"
     CDS             18631..19020
                     /locus_tag="PKX59_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ90456.1"
                     /translation="MITTPLKIFIVDDDNMMLEMLRDRIGEHTPHTVLTFGTGEDCLK
                     HVAENPDVIILDYYLNSKNPDASNGMEILKAIKKYNPNIHVIFLSGQERYGVAMQTLQ
                     KGAEYYVIKDNEAFGKIEKILSGITVS"
     gene            complement(19070..19525)
                     /locus_tag="PKX59_06005"
     CDS             complement(19070..19525)
                     /locus_tag="PKX59_06005"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOZ90457.1"
                     /translation="MMNETVRVTKIFVFDMAHALFGYDGPCKNIHGHTYKLSVTLKGK
                     PFNDNSNPKAGMVIDFSILKQIVQEKIIKQFDHALVLNNDSPHKLLDKVLTQQFEKII
                     YLDVQPTCENLLLHFKKQLASHFADKIKLVYLKLEETPTSYSEWCIDDN"
     gene            complement(19525..20034)
                     /gene="paaJ"
                     /locus_tag="PKX59_06010"
     CDS             complement(19525..20034)
                     /gene="paaJ"
                     /locus_tag="PKX59_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011537332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HOZ90458.1"
                     /translation="MSSVKNTESSKSTESLIWALLHTVPDPDIPVISIVDLGIVRNVE
                     WKNEKAIITITPSYSGCPAMSVFVDDIKQVLNGAGFQSEIKTILSPAWTTEWISQSGL
                     DKMKAHGIAPPEDETGMDSIFAMQKPVTCPRCNSKQTKMLNRFGSTPCKALWYCNECS
                     QPFEYFKCH"
     gene            complement(20046..20804)
                     /gene="paaC"
                     /locus_tag="PKX59_06015"
     CDS             complement(20046..20804)
                     /gene="paaC"
                     /locus_tag="PKX59_06015"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HOZ90459.1"
                     /translation="MKTKDALYNYCLRLADSNMIHGQRLAEWCSRGPFLEEDIALSNM
                     SLDMFGQAEMLYEYASSLSEDELSADDIAFRRSEREYFNCLLVEQENGDFAQTMMKVF
                     LYSAFAKQLYESLLNSNDETLSGFAAKAIKEVKYHLRHSSEWLIRFGNGTDESKMRVQ
                     QAVTNLWRFTQDMFEMNDADTALIDNGISFTTDTLFAKWQLNINEIFAAANINTPEVN
                     NTITGGHNGIHTERLGVMLCEMQYLQRAYPDAKW"
     gene            complement(20807..21097)
                     /gene="paaB"
                     /locus_tag="PKX59_06020"
     CDS             complement(20807..21097)
                     /gene="paaB"
                     /locus_tag="PKX59_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HOZ90460.1"
                     /translation="MKNDNQFPQWEVFIQTKNGGPFEHQGSLHAADKKMALQNARDLY
                     TRRNEGRSIWVVPTECIAATTADDAADFFDPANDKIYRTPNFYKMPQGTKNL"
     gene            complement(21109..22056)
                     /gene="paaA"
                     /locus_tag="PKX59_06025"
     CDS             complement(21109..22056)
                     /gene="paaA"
                     /locus_tag="PKX59_06025"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HOZ90461.1"
                     /translation="MTEEEKEKQFQQRIDAGEKIEPRDWMPEAYKQTLFRQISQHAHS
                     EIIGMQPEGNWLLRAPSLRAKKILLAKIQDEAGHGLYLYSAAETFGIDRTEMIAMLHS
                     GKAKYSSIFNYPTLTWADMGAIGWLVDGAAIVNQTMLAKCSYGPYSRAMIRICKEEGF
                     HQRQGYEIIAKMMNGTTAQRNMAQDSLNRWWEPSLMMFGPHDSESTHSKDSMKWKIKV
                     ESNDTLRQRFIDRTVEQAHHIGLTIPDKNLKYNKTTMHWETSEINWDEFWSVVKGNGP
                     CNYQRISHHIHYHNEGKWVRDCATAFEKSREKENSSISI"
     gene            complement(22068..>22263)
                     /locus_tag="PKX59_06030"
     CDS             complement(22068..>22263)
                     /locus_tag="PKX59_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HOZ90462.1"
                     /translation="KKWGIMVDRDEEGYLLQIFTKPVEDRPTLFYEIIQRKGAKSFGK
                     GNFKALFESIEAEQARRGTL"
BASE COUNT         6783 a         3886 c         4377 g         7207 t
ORIGIN      
        1 gttagaggtg caactgtaat tttaagacag gatgctgttg taaacccaac tacagtgggt
       61 gcagggggtt taggagtttc tgctgctgat ggaggtaatg ggggcaattc atcgtttgga
      121 ggttcagtga cagctaatgg tggtaaaggt ggtaaagtag gttatttttg ggcacaatac
      181 ggtgctggag gtgcctcaac agcaggaaca cacagtgggg gtgccggtgc agcaggtgcc
      241 agtggtggct caggaggcgg aggcggaggt gccggttcaa caggaaatgg tggcaatgca
      301 tcaggaacaa caggtggtac aggtggtaca ggtggtggag gtaccggtgc aaatggtaga
      361 acaagtaacg gtgatggaag tgatgcaact gcactttcag caggtggtgg cggtggccgc
      421 tctaatgcta gtaatactga tagaataggt ggtgatggat ttagggggca gataaaagta
      481 acctatacct gtgtatctta tagccttact gctacaagtg gtacaacacc aatttgtgct
      541 tctgcaggta cttcagtaat ttctttaaca ggaacagcag caacattgcc tgttggcgat
      601 tatgtcgtta cctatgacag aagccttcct tcggcaagta atttaacagt ttctatggca
      661 gtggttacag caggtacagg ttcgtttaca gcaacaggtt taacttcagg aggtaacagc
      721 acaattacag ttactaaact cacatcatct tattgtaata acaatatcac tttaaataat
      781 acaactgtta ttgcagttgc tgccagtatt cctcctcaac ctgatgttat tgcaggtgca
      841 cctgttgatg tttgtcctcc aacagcaagc tacacactta gcacaaacag cacaggtgcc
      901 gattcatata actggcattt aggtgttggt gtaaccggtg ttactttttt aagtgctaac
      961 ggaaataata ctatggatgt acagtttgga gcaacagcta actcaaccta tgttattcgt
     1021 gttgatgcaa ccaatgcatg tggtacttca gcttatcgtc ctgtaatgat aagaagagca
     1081 acctctgttc cagctgcaat taccggactt aaaactgcct gtgccagcac atctgatact
     1141 tatacagcaa ctgcagttgc aggtgcaaca tcgtatgagt ggtctattag cggagatgca
     1201 actgtgacgg gaactactaa cacagcaaca gtaaacttcg gtccgacctg gacaggtgga
     1261 acactttgtg tagcttcaaa agtaggttgc tttaccagtg ctactaaatg catgactatt
     1321 tcaacatctg caacagtttt aagtactgtg cttggtggaa catttaaagc atgtccaaac
     1381 tcatcgctta actttaatat tacagcatca cccggtgtgg ctacttacaa ctggactgtt
     1441 cctgccaatg catctataaa cagtgggcag ggaaccaata ctatcactgt tgattttggt
     1501 ccaagttata acaacacagg aaatatttgt atgacagcaa ccagcatctg cggtgttgtt
     1561 gcaccaacaa aatgtaagag tgtttctccg ggattgcctt ctgtacctgc aagcattaca
     1621 ggtggtacca atggtttgtg taatcaaact atgccatttt ctacaccaac aatggcaggt
     1681 gtaaccagct ataactggac agcacccgga actgtaaata acaatggaaa taatgcggta
     1741 acaattgatt ttggtgcttt aacaacagga caggtttgtg tgagtgcaac caatagctgc
     1801 ggaacaagca acacgcgttg tactgctgtt aaaggtgcac ctaatacacc tgcttcaatt
     1861 actgcaacac caagtacatg gtgtcctaac actgcaggag ttgagtttaa cgccaatatt
     1921 gctaatgtaa caggtgctta tactttaagc tggacatatc ctgcagcatc tgcaaattat
     1981 atttcaggtg gtggaaactc aagttcatta attcttgatt ggacaacagg caacggtgca
     2041 gtaatgatta cggcaactaa tgcatgtgga tcgggtacaa aagtgtttga tgtagtatta
     2101 gacaactgcc gcgaagcagg aattgaaaca gtaaaacaaa tggatgtgta tcctaatcct
     2161 gcaaatgatt tggtaaacat ttcatatcaa tcagcagcaa cagagttgat aaaagtttcg
     2221 ttgatggatt actccggtaa acttgtagta aatcaaaatg tccaagtaaa ctcaggcatg
     2281 aacaactatt cattagatgt ttctaaactt gctcctgggg tttatatgtt aaacgtacaa
     2341 tcagaaaacg gcaatatgca acgtaagctg ataaaacaat aattaattat tttaaaatta
     2401 acaaggctgc tttggttacg aagcagcctc gtttttttta gtttactttt caaaaaaact
     2461 aaaccaatct tttagcctgt cattttttca atggcatttt tcacatcctt catcaaccat
     2521 agtggtgttg aagtagctcc acaaatacca acagaattaa ttccttgaaa ccaagtggca
     2581 tccaactctt cagccgaaga aataaaataa cttttgtcgt tggtagcttt acagacactg
     2641 tataaagctt ttccgtttga tgattttttt ccgcttacaa aaactacaac atcatgttgt
     2701 gatgaaaatc tgcgcatctg tggctcgcgg ttgctaacct gacggcataa agtgtcgttg
     2761 gtaataaaat cggtttcttc aagcgagcct ttttgtttta ctattcgttg ctgaatggtt
     2821 ttttgcattt gttcaaaacc aagtgtgctt tttgttgtct gagaaaaaaa atgaatcggt
     2881 ttattgaaat ctatctgatc aagatcttct tctgttttaa ctacaatggc ttttcctccc
     2941 gtctggccca ccaagccatt cacttctgca tgacctttct cgccataaat caccacctga
     3001 ccatttcccg tatctgtaat ttcatcataa ctgcctctga cacgattttg taacttcagc
     3061 acaacaggac aagatgcatc aataagctca atattgtttt taattgccgt ttgatatgtt
     3121 gatggtggtt cgccatgtgc tctgattagt actttgcaat cgtgcaactg ttgcaactgt
     3181 tcatgattga taatttccaa acctttacct ttaagtctgt caacttccat gttgttgtgt
     3241 acaatgtcgc caagacaata taagtgaccg ttaacttcaa gctcttcttc agcctgctgt
     3301 atggcataaa ctacaccaaa gcaaaagcct gaattggaat caatagtaac cnnnnnnnnn
     3361 ngcatagtga acaaattatg tgcaaaagta agacaataac gttttaaacg ccctatggtt
     3421 taatcttatg acatttctat ttcaaaacca tcgtaagcta aaaacacatt tggcggtaat
     3481 tccttttcta catcactgtg ttttccaagc tgatggctga tgtgtgtgag gtaggctttt
     3541 tcgggcttca attctttaat caactcaagt gcttcttcaa gattaaaatg agaaacatgt
     3601 ttttcgcgtc ttagcgcatt gataactaca atacgactgc cttttatttt ttctttttct
     3661 ttttctgaaa taaagttagc atctgttatg taggtaaaat caccaaccct ataagcattg
     3721 acaggtaatt tgtaatgcag cacttcaatg ggaataaact ccagtccttc aatattaaaa
     3781 gggtgctcat caatggtaat catattcact tcaggcacac ccggatattt aaagtcatga
     3841 aaaacatagg ggaactctct aactaacgct tcctgaacac gcaaagtagc atagatgtca
     3901 atttttttct gcaacacata gttgaatgcc ctgatgtcgt ccatacctgc aacatgatct
     3961 ttatgctcgt gggtaaaaat taatgcagta aggtttttta tctgcgcccg caacatttgc
     4021 tgcctgaaat cggggccgga gtcaatgaca aaactgctgt tcttatgctg aatatgaatg
     4081 gaagtgcgca aacgtttgtc gcgcaaatca gtactctggc atacattgca attgcaggct
     4141 attaccggaa cgccttgcga agtgcctgta cccaaaaaag ttattttcaa tgttcagatt
     4201 ctgatgaatg aggtatggta actacctgtg gttcgggatt ttccattgct gcaaacaact
     4261 cctgcctttt gttgctgagt aattgttgcg attcaagaag gttgatgatt tcaagtagtg
     4321 cactgcatcg tgtttccatg gcagaaaatt tatttaccat cactaacttt ttggattcca
     4381 gcagacaata gccattttta aagttgcctt tttcgtagcg tacatcgtag ccgttttcca
     4441 ataaatgctc ttctaatttt tttaaatact gtggtgtaaa cttagccata tcgtttttag
     4501 aggttcttag tgccaaaatt attgagtatg atgttcacta ttttgctttg gcagggagtt
     4561 ttattaacaa cggacaacga tgataagaga ttgcatataa gccgaaaatt attttctttg
     4621 caccgctttc tgataaattg ttaatcaaaa aattaagggc atgattttag gcgtacccaa
     4681 ggagttaaaa aatcgcgaaa ggcgagtagc actaacccct gatgttgtta aaagtttagt
     4741 agcttccggc tacaatgttg ttgttgaaga aggtgccggt gaggcttcct tttatactaa
     4801 cgaccagtat aaaaatgcag gtgcaacact tggcgacaag gcacaggtat atggtagtga
     4861 tgtagtgttg aaggttaatg caccgcaacc tgcagaaatt gctcagatga aaaagggtgc
     4921 tacactcatt tcatttatgt gggctgctac caatgcagat ttagtggaag cgtgcaagca
     4981 agcaggcatc aatgcgtttt ctatggatgc tattccacgt atttcaagag cgcagaaaat
     5041 ggatgcgcta agttcgcagg caaaccttgc gggatataaa gctgttgttt tatgtgcaga
     5101 tacattaggt aaaattttac ccatgatgac tactgctgca ggaaccattc gaccctcaaa
     5161 ggttgtaata tttggtgcag gtgtagcagg cttgcaggca attgctactg caaaacgttt
     5221 gggtgccgtt gttgaagtta gcgacatcag acctgaaact aaggaacaag ttcagtcact
     5281 cggtggaaag tttattgaag taaaaggtga tgacagtata aaaattgaag gtggttatgt
     5341 gaaaggtgtt tcagaagagt ttttgaagag gcaacaggaa ttagtagcca aacatatcaa
     5401 agaagcagac attgtaatta ctactgcatt aataccgggt aagaaagctc cattgttagt
     5461 tacagaagag atggtgaaga gtatgaaaaa cggttcagta attttagata tggctgttga
     5521 gcagggagga aatgttaccg gaagtgagtt aaatcaaacc gttcagaaaa atggtgtaac
     5581 aattattgga gagagtaaca taccaagttt gttgccgatg aatgcaagtg atttatatgc
     5641 aaagaatatt cagacatttt tacttcacct aacaactaaa gacggattta agtgggaaat
     5701 ggaagaagag ataacaaaag gaagcttaat agttaaaagt tagagaattt ttgaataaaa
     5761 gaattgtgga gttgatgatt taggaaattg tatagttttt tgtgaatgga agtaacatta
     5821 aataaaaaaa caaaaaaaga ccaattttgc agaggaatta aaaaggcgaa ctgaaatatt
     5881 ttcaacatca attataaaat tcacaaaatc atttccccaa aagtagaatt caatagctca
     5941 aaagaaaaaa caactcaatt attcagaaaa ttaataatta aacaacatga cagagataat
     6001 taattttata ggaacacatc aggagattat attttttata attctttctg tatttgtggg
     6061 tatagaagtt attggcggag tacctacggt attacacaca cctctgatgt ccggtgcaaa
     6121 tgccatacat ggagtagtta ttgtaggagc cataattgta atgctcaatg ttgacgctaa
     6181 cgactatgtt gcacttgcat taggctttct tgcagtagtg ttaggtacat tgaatgtggt
     6241 aggtggattt gttgtaacag accgcatgtt ggaaatgttt aagaaaaaat aaaaccacaa
     6301 agaagaatta aagatatcag cgcatgacac ctgaagtatt atataacatt cgtacaatta
     6361 catacatcat agcatctgct acgttcataa taggtcttaa acgtatgggt aatgccaaaa
     6421 ctgcacgtaa cggtaatctt attgccgcag taggtatgac cttggcaata attgcatcaa
     6481 tatttattca tgtaaacaaa acacaaactg caaccggtgt aacatttgaa caagttgaaa
     6541 caccagggtt tatttacaca ttaatttttg ctgcaatcat tatcggaaca atcattggct
     6601 ggcttactgc taaaaaagtg cagatgacaa aaatgcctga actggtttct atgttcaatg
     6661 gtatgggtgg tgcctgtgca gcgcttattg gtctcatgga gtttcatcat aatacaggaa
     6721 acaccggtgc tttaattgct attgtgcttg ggttgataat cggtagcgtg tctttttcag
     6781 gaagtgttat tgcattttta aagttgaatg gtacaatgaa caaacctgtt cgcttaccat
     6841 catacaacat cattaacaca ttagtcatga taggtgtatt tgcttttggt gcatacatga
     6901 tttttagtgg aaccacaagt gcaacattgt tgtatttact ttttgcagca gcattgatat
     6961 atggaatttt gtttaccatt ccaatcggtg gtgccgatat gcctgttgtt atttcattgc
     7021 ttaactcatt cacagggttg gcagctgcat ttggcggttt cctttacgat aataaagtaa
     7081 tgcttaccgg tggtattctt gttggctcgg caggaacctt gttgacactt gtgatgtgta
     7141 aggctatgaa tcgtcctttg agtaatgtaa tttttggagc ctttggcggt ggtggtgctt
     7201 cagcaggtga tggacctgat gttaaaggaa gtattaaaga tatgtctgtt gctgatgcag
     7261 ccgtcttact taattactct aagaaagtag ttattgttcc cggttatgga ttggcagttg
     7321 cacaagctca acacatcatt cacgaactgg aaatgttgct tgaagaacgt ggtgttgaag
     7381 taaaatatgc tattcacccg gttgcaggtc gtatgcccgg acacatgaat gtgttacttg
     7441 ccgaaagtaa tgttgattat ggaaagttgg tggaaatgga agagataaat cctgaattgg
     7501 aacaaacaga tgtagtgttg gttgttggtg caaatgacgt tgtaaaccct gctgctaaaa
     7561 cagatccatc atcaccaatt tacggtatgc ctattctgga agttgataaa gccaaacata
     7621 ttatagttaa caagcgatca atgaatgccg gctatgcagg aattgataat ttactgtttt
     7681 acgatccgaa gacaagcatg ttgtttggtg atgctaaaaa agcattgact gaattggtgg
     7741 ctgaattaaa aacaatgtaa gaacagtttt tcttggtaaa ggaattttta tattctgata
     7801 ccgattatca aaacatcatc aacttgttcg ttgttgcctt tccactcatt aaagtaattc
     7861 tgtaaatata ttttctgttc attcatgcta aggtgctgaa tagaaactaa aatttctttt
     7921 agttttttag tcatcaattt tttaccatgt tcaccaccaa actgatcggc atagccatct
     7981 gaaaaaagat aaatggtatc ctttggctgt atttttattt ggtgatttgt aaactgattg
     8041 ttgtgtttta tctgtagtcc accaatcgga aatttgtctg ctgcataaac aattagttca
     8101 tcatttctga tcagccacaa tggtctgttg gcaccggcaa attctaagct catatttttt
     8161 gtatcaaaag aacagataga aatatccatt ccatcatgtg tttcgctttc atgttgttta
     8221 agcgaattga taataccttc gttcaaagca tctaaaatcc ccgatggcgc agtaatattt
     8281 ttttcgttga tgatagattt aattaatgag cttccgataa tactcataaa agcacctgcc
     8341 acaccatgac cggtacaatc tgctgcggca ataatatgtt tgttttcttt ttgtgtgaac
     8401 tcataaaaat cgccactgac aatatctttt ggcagataga gaataaatga ctggtcaagt
     8461 attttataaa cagtatttaa gtttggtagg actgcggcct gaattctttt tgcataaatc
     8521 agactgtcag taatttcttt gtttttgtta aggatttctt tgtttttctc ttcaagttca
     8581 aaggttcttt cagttacttt gttttcaagc aaaatgtttt gttgtgccaa aatatttttc
     8641 ttttcggttt cttgtaataa attttcttcg cctaattttt ttatggtttg aacctgttct
     8701 attaactgtg tttgattttt cccatagcgc cttgccaata caatagcaac agttatggga
     8761 taaacaccag cactgattgt aaaaagtgta tttaaaatat tatctgaaat gttttgttga
     8821 tcaaaacttt tattcattgc atacccaata gggagtagaa aaagtaaaat tcctatccct
     8881 gtgccataag ttaaaagttt tgcatcccag tttttgtgac cgaaaccttg tatccaatga
     8941 tatgcaaaag aaaataatga gtaagagaga aaaattacat aactcaaaaa cctgataacc
     9001 ggtatttcaa tattcttaac gaatttaatg gttacaataa aatcatcagg aaaaattaag
     9061 ctgaatatta aaactgcaat cattaaaaat aatggaattt ttgttttatg tattttgtta
     9121 atggccaaag ccataaaaat ggcaagcagt tcaagtgaaa ttaaaaacga gtaggcagaa
     9181 agaactttaa ttgttggtgt ttgtgtcagg acttcagata gatattgcaa gaatgtagct
     9241 aaacagtaaa gaccaaagaa taaatattcc tggtttttga taaaaaagta aaaaagtatc
     9301 aatactatgg caaaagaaaa atagaaagta gccacagcat aacttctgat ttcaccatcc
     9361 ttcttatctt ttagattacc ggcagcccat tttgcatctc caagtctaat gttgagatta
     9421 tgccttttca gagcgggcag atattgtact tgtaaaacat ttttattttt tttgagtata
     9481 aaatatttat aatctttttg ctctgatttc acatcagtat aatgtttatt accatcaaac
     9541 acgccacata atgccactac ttcattattg atagaaattt ttacagatcc atttgtgtta
     9601 taaaaaagtg cctgaacttt atttaacatt gaactatcaa cataaaaaac agtagtcagt
     9661 agagcacctt caggtatttt gttttgaatc tgaatagtat caacctgatc ggtataatat
     9721 tttttcgtta agccattttt attgtattgt tcaacattcc attcaaaatt acttaatgca
     9781 tttatgctga aaacagtatc gcgctttgga gatttagatc cgaaaaaaga aagcgtgtca
     9841 actttgtttg caacagcaaa gtcagagctg aaaaacaaac aaataaatgc cgataagcgt
     9901 agaaaaactt taatcagttt aacttttgaa agcatatatt aagattgact gggcgaaggt
     9961 aaagtataat tatttaatcg gatatataac ttattcagat agtttttaaa ttatacttat
    10021 ttttacatca gatttcctca gtgtattttt caaataccaa atcggcacat tcgtgttcag
    10081 atagatgaga ttagttttat tataaaacta aaataaaaat aaacataaaa actaaaaaat
    10141 ttgtttaata aagatgatta cagttattat accgacactt aatgaagagg agaatattgc
    10201 aagtgttata aaatttgcaa aacaaaattc attggtatca gaagtattgg ttgttgatga
    10261 caagtctatt gataatactg taaggattgc acaggataat gatgcaaggg taattaccag
    10321 tacaaaaata ggaaaaggag catctatgcg cgaaggtgtc atgttttcaa aaaatgaaat
    10381 tattgtattc ttagacgcag atatcaaccc ttatccgcat ttcactatta aacttttaac
    10441 agatccaatt attcatagtg gtgccggatt tgtaaaatcg tcatttaacc gaaatgcagg
    10501 aagagtaaca gaattggtag caaaaccaat gttaagcatc ttttttccag acttattaag
    10561 attcaatcag ccattaagcg gaatgattgc cagtaaaaaa tccttgcttc aacaattgga
    10621 ctttcgtgat gattatggtg tagatattgg attgctgatt gatatgcatc tgatacatgt
    10681 aaatatcagt gaagtagaaa taggttatat tgaaaacaaa agtaaaccct ggcaagcttt
    10741 aggaaaaatg agtaaagaag ttgcacaaac tataattatt aaagcatcat catcaggtaa
    10801 tccacattat aattttgaag agtttggtgt gctgagtgaa attcgttcac aaatggaatt
    10861 cgcattaaat aagcaacagg agactttcaa taagttattg atatttgaca tggataacac
    10921 acttctgcgt ggtagattta ttgatacctg tgcggaaagg tatgggttta aaaagcagct
    10981 aatggacatt cgttcttcag aaacggatag taccataatt accaaaagaa tagcgaagtt
    11041 gttaaaggga aggacaatag atgaactcat agctatagca gatggtattt ccattgttga
    11101 tgacacagaa aagattatca caaaattgaa aaaacgtggc tatgttgttg gaattatttc
    11161 tgacagttat gattgtataa ccaatcatgt tagaaataaa ttgcgaatgg atttttcact
    11221 ttctaatgaa ttagagtttt caaaaagtgt ttgcactgga gaggttaaaa taccctcact
    11281 gtttgtgaat aatgaaagca gtagttgtaa acatgttttg tgtaaaacca atgcattaca
    11341 aaaattactt gaaaaatacg atattcaaaa agaaaatagc attgccattg gagacagcat
    11401 gaatgatctt tgtatgataa aagaagccgg cttgggaata gctttttgct ctaaagatga
    11461 attgttaaat caccatgctg atgtaataat tcgtaaacct gattttagtg cattgctgaa
    11521 cattgcaaaa taattttgtc ttttttgatt tagataaata gaataattga aattctttaa
    11581 tctatcttgt tccgaaaccg acaaagtgtt taaagaaaag actaaaaccg atatcccatc
    11641 gcaaaactaa actgcacatt aggttctaca ggtgtagtgt caaacgagtt ctcatattca
    11701 ttttcttcgt tggaatcata atatttaatt cctaatccca gttccatacc aatatatagc
    11761 gttttagcga ttgatacatt caggctgtta ttaatcataa aacccaactg tgtatgattg
    11821 tctgaatatt tgttattaac tgtgatatat tgattgccat tccaaacagt attgtaatca
    11881 tgatgattaa tttgtccgct ggcaatataa aactgtggcc ctacagcata ccttaccaca
    11941 ccttgtcttg tagggtagag tttaatcgtg ggcatgatgt aaaaaaatcc cgaattacgt
    12001 aatgaaatgt taacggggat tttaatactc accaatcctt cgtttagtat tctctcatag
    12061 ttaagtccca cagctacatc ggtattatta tccatagact cattgccaat aatagccatt
    12121 ggtgtaagag aaaaaatgtt atttcccaat tcgggtttgt cctgggcaaa tgttgatgta
    12181 gataaaaata gtgcagatgc taagagaaag gttttcatat ttttatgtta ttttaaaagc
    12241 taaagtagtg tttttaagac ttcaaaaaaa cggcatttaa attcaaaaat tgtatggttg
    12301 gcactttcag aatttcagta cattcgttac ctttgtaact attaaaaagc ggaattatgt
    12361 cagaccaaac ccaaataatg cagaagaaag agctttcatt tgaagatttc agacaggtgg
    12421 tgctaaacga ctatcgttta gggcgtatca gccgagaaac cagtttgttg ggacgcagag
    12481 aagtgctgac aggcaaggca aagtttggca tttttggcga tggaaaagag gtggctcaga
    12541 tagctatggc caaggttttc caaaatggcg atttccgctc cggttactat cgcgatcaga
    12601 catttatgat ggcagcaggg ttgctcacag ttcaggaatt ttttgctcag ctttatgcgc
    12661 atccttctgt aatggcagag cctgcaacag caggtcgcgc catgaatgga cactatgcta
    12721 cacgattact gaatgatgat ggtagctgga aaaatcagac acaatcaaaa aattcttctg
    12781 ccgacatatc gccaacggca ggccaaatgc cacgattgct cggacttgct ttagcttcta
    12841 aattctatcg caataatccg gaacttaagt cggataagtt taaaaacttt tctgttgatg
    12901 gtaatgaagt tgcttttgga actattggtg atgccagcac ttctgaaggt ttgttttttg
    12961 aaaccatcaa tgcagcaggt gttttgcagg tgccaatggt tatcagcatt tgggatgatg
    13021 gttatggtat ttctgtgccc aataaatatc agacaacaaa acaaagcatc agtgaagcat
    13081 tacaaggttt tgctcgtact caagaaaaac ccggttacga aattattaga gcaaagggat
    13141 gggattatgc agaattgtgt gatgcttacg aacaggctgc taacattgca cgtacagagc
    13201 atgtgccggt tatggtgcat attcagcaaa tgacacagcc gcaaggtcac tctacatcag
    13261 gatcgcatga gcgttacaaa tctaaagagc gcttgttgtg ggaagaagaa tttgactgtc
    13321 tcagaaaaat gcgtgagttt atggtgcagt ctgccattgc aacagaacaa gaactcgatg
    13381 ctatagatgc tgaagccaaa aagtttgtta atgatggaaa gaagcaggca tgggatagtt
    13441 ttacttctga aattaatact gaaaaacaac aggcactttc actggcagaa gcagcaatca
    13501 gcaaaagttc aaatggtgtt tttctgaata aagcagttga agagattaag aatatcacca
    13561 attgcgaaag aagagatatt gctgttttta tacagcaagt tttgtttacg cttcgtgctg
    13621 ataatagtgc tgagatagaa gcactgaagc aatggtaccg ccaatggcat cagttgaatt
    13681 ttgatcgtta cagctcacaa ctttatgcag aaggtaacgg cagcgcattg ggcattgccg
    13741 aagttaaggc tgtatattct gcagaggcaa acatggttga tggtcgtgaa gtacttcttg
    13801 caaatcatga taaacttttt agtcaatatc ctgaatacat tgcctttgga gaagacgttg
    13861 gaacgattgg tggcgttaac caaacttttg caggcatgca ggccaagtat ggcgagcatc
    13921 gtgtttttga tgtaggtatc cgtgaggcaa caattgcagg acagggaata ggaatggctg
    13981 tgcgaggctt gcgaccactt gcagaaattc agtatctcga ttatttggct tatgcaatac
    14041 agattttatc agacgattta gcttgtttgc gttatcgcac caaggcagga cagaaagcac
    14101 cactcattgt tagtactcgt ggacatcggt tagagggcat ctggcattct ggttcaccta
    14161 tgcagtttat tcttggtgct ttgcgtggta tgcttgtgct tgttccccgc aacatgacac
    14221 aggcagcagg attttataat ttgctattgc aatgtgatga tcctgcattg gtgatagaac
    14281 cattgaatgg ctacagacta aaagaaaaac tccctgagaa cctaggtgct tttactgtgc
    14341 ctgtcggtgt gcctgaaatt cttgaagaag gtaatgatgt tactctggta acttacggtt
    14401 catgtgttag ggttgctcag gatgcaatga aacaactgca gcaatgtggc attagtgtag
    14461 agttgataga tgtacagtca ctcataccat ttgacattca tcatatcatc gctgaatcta
    14521 ttcaaaaaac gaataaggtt gttttctttg atgaagatgt tcccggtggt gcaactgcat
    14581 acatgatgca acaggttatt gaaaatcaaa aagcatttga gtttcttgaa acaccgcctg
    14641 taactcttgc tgcaaaagaa catcgtccgg catacggaag cgatggagat tatttttcga
    14701 agcctaatgc ggaagatgtg tttaatactg tgtatgaatt aatgcactct gtaaatccac
    14761 aggcatttcc attgttcaga tagtcaggat aaacttagtt tctctacccg attaatttgt
    14821 cgggattatc agaaataaat ttttgccatc ctttgctttt gcctcccgca aactgacttt
    14881 gcccggtatt tttctgaaag tgatgacata ctgcagctgc caggccatcg gtagcatcaa
    14941 gatattttgg catttcttca aagtgtaaca actgttgaag catagcagca acctgctcct
    15001 tggtggcact accattgccg gtgacagatt gttttatttt ttttgggctg tattcaaata
    15061 caggaatatt tttgaataat gcagcggcaa gtgcaactcc ttgtgcccta cctaacttta
    15121 acatagattg tacattcttg ccaaagaacg gtgcttcaat tgccatttca tcaggcaaat
    15181 agctttcaat aaggcgtaat gttttctcaa aaatatgctt taatttcagt ggatggttgt
    15241 caaatttttc gagatgaatt atacctgcgg tctctaattt catttctgtt cccgaaataa
    15301 ggagtactcc atatcccatt acattagtgc ccggatctat tcctaaaatt attttttcgc
    15361 tttctgacat gaacgctatt accgcttatt ttttaaactt gcactataat ctgcaattgc
    15421 ttggaaagtt caaaaataaa gattaaaagt gtgctacagt tttttctgaa agcaacaatt
    15481 gcattgtttt ctctttggtt cattgttcat aaaattatac accgcgagaa tgaagtgtcg
    15541 tttttcgatt ttgtgaatac tcgcctgcat acagaaggtg cttattcctt gctcatatct
    15601 gttattgtat tgatggtgtt gaattggagt atagaagcta taaagtggaa ggtcttaata
    15661 aatagactca ccaaaattag ttttgttcga agcctaagtg cggtttttgc cggggctact
    15721 gttagctttt ttacacctaa ccgtgttggc gactatgcag gtagaatgct ttttttgcct
    15781 tcatctgttc gcatagcatc actgctttca acttttgcag gaaatatcag tcagctcatt
    15841 gttacacttt gttgtggact tgctgcaatg gctttgtcat tacaacatta tgtacagtgg
    15901 aatgaaagtt tagtcgtcac aacacgtgtt gcatcatttg tttttgcatt gatacttttt
    15961 gtttcttttc cttatataag aattgttttt aaaatgcctt tgctgaattt tgttcgacag
    16021 cgatttcctg aatatgtcat gcatcttgaa aattattctt acagagaaat gatgcacata
    16081 ctttttcttt ctctgctgcg ctattctgtt tttgcttttc agtacatatt gctatttctg
    16141 gtttccggca ttccggttat tccatctatg attgtcatta tagcactcat cttttttatt
    16201 caggcattgg ttccaacact tgcactgaca gagtttgcaa taagaggttc tgttgctttg
    16261 atggttgcag caccctttat tcagaatgat caggcagttc taatggcatc atttgcactc
    16321 tggctgatta atctggcagt gcctgcagca attgggtcgg taatgatttt ttatttaaat
    16381 ttcagaaaag aataacgctc attatttgat ttcccaaaca gcgatagtaa gatgtttatt
    16441 atcaaaagta cttacacccc aaactttctt ttgtttgata ttggtagcat agtccaactg
    16501 cactgcctca tcatgtcctt ctgccaaagg ccatgtctgt aaagtgtggt ttttaaaatc
    16561 ttcaaaatat aaagtcagtg aacgatcaaa ataaactgcc gtttcttttt ccagatcaac
    16621 taaaagcggt ttgcatttat ttgtaagtct gtcgctgaac caaaggtcgc tacgctttaa
    16681 tgcaagatag gtttttctgt taccgacttt tttgataaaa ttataggcaa actgatagat
    16741 gtagccatcc tgatagttta accacaggtt gtatgagtaa tgaaagcgtg ttttataaaa
    16801 atcctgatag tattcgggat tgttatagta agtaggaatt gaagcaattt caaaaaaacg
    16861 ataaaaatga ttcagacttt tataccagtc ttcgggtttg ttataaggaa tgacttctat
    16921 tttttttgtc ttacgaacat atttgaagat tcgtgtttct tcattgtcca tcatcatcag
    16981 gttgtcaaac tcatcaaact ccatgttgcg caaacaacct tcgtaaggaa gtttaatttc
    17041 ttcaagaatg gttccctgat tgttaattaa aaaaacataa ggctggttac aagaattgaa
    17101 tgcaattatc tcatcggcat ctactgcaat tctgtatgtg aaaattccac cgtcaatcac
    17161 tctgtcgcgg tcaagtgtaa aaaggtctgt cttaacaaag gggccacgtc tttcctgact
    17221 ataacctgaa atgtaatgtg taagaagaat aacgaaagaa ataataaacc gcatgtaaac
    17281 aaaaattaag gctgcaaaag tcctcttttt attcatataa agccacaaaa agacaggtaa
    17341 aatcttctct atttgaagtt aaaatgttga cctttgcaca attgatgaat actgttgttg
    17401 ccattatttt ctggatttcg cttttgggac ttttacattc ttatgtactg tttccattac
    17461 tcatgcgttt acttttaatg ttaaagcaca agtctaaaga tgatgatgac aataatttat
    17521 ttaagccgat ggtcagcgtt gtgatggctg cttacaatga agagaaggtc atcattcaaa
    17581 agctcgaaag tgttattaaa agtaattacc catcagataa attagaaata attgtaggct
    17641 ctgattgttc caatgatagt actgatatgt tggtgaaaga gtttgcacat aatcatccac
    17701 aagtaaagtt tattcgattc aacagcagaa caggaaaacc tgcaatcatc aatcaattgg
    17761 taaaacagtg taagggtgag tttttaatta ttactgatgc caatgtaatg tttgatgctg
    17821 aagcaatttc atctatgatg catagattta ctgtagcaga agttggctta gttgatagca
    17881 acatggtgaa tattggattg atgaaaagcg gaatttcatc tcaggagaaa cagtacattc
    17941 aaacagaagt tttaaccaaa tatgccgaag gtgaagtatg gggaagtatg atgggccctt
    18001 tcggtgggtg ttatgcaata aggaaaagtt tattcagcga tattccttca aactttctgg
    18061 ttgatgattt ctatgtctgc atgaaagtac tggagcaggg ttatcattct gtaagttccc
    18121 taaaagctat tgtctatgag gaggcatcgc ataatctttg gatagagttc aggagaaaaa
    18181 cgcgtatagc cacaggaaat tttcagaatc tggcaaagtt tagcaagatg atttttaagg
    18241 gcagaggtat tggattttgt ttcatgtctc ataaagtttt gagatggaaa ggccctgtgt
    18301 tgatgttgct tttatatttc tcttctattg cggcctggtg gttgtctatt gatataaatc
    18361 ctgttttgta tctgggagtt tttgcagcca tatcattaag tttacttttg ttgctggcag
    18421 acaggctact taatttgttt caaatacata taggattgct cagattaaat acacatttct
    18481 tagtcatgaa tgctgctatg ttgaagggta tgttcagata cattaaagga gttaaaagcg
    18541 gtgtttggca acctacaagc cggactcaga atgcttaatt ggatattaca aaaaaattgt
    18601 aggtttgtat tccatttgac actaaacaaa atgataacaa cacctttaaa aatatttatt
    18661 gttgatgatg ataatatgat gctggaaatg cttcgtgaca gaattggtga gcatacacca
    18721 catacggttt taactttcgg cacaggcgaa gattgtttga agcatgtagc agagaatccc
    18781 gatgttatta ttcttgatta ttatctgaat tcaaaaaatc ctgatgcatc taatggtatg
    18841 gaaatattga aagctatcaa gaaatacaat ccaaacattc atgtaatttt tctttcggga
    18901 caggaacgct atggtgtggc tatgcaaacc ttacaaaaag gagcagagta ttatgtcatt
    18961 aaagacaatg aagcttttgg taaaattgaa aaaatattaa gcggcattac agtttcataa
    19021 atcaactcta aaaagttgat actgaaattt tttattcacc gcttagttat cagttgtcgt
    19081 caatacacca ctcagaataa gaagtaggag tttcttctaa ttttaaataa accaatttta
    19141 ttttatcagc aaagtgagat gcaagttgct tcttaaaatg caacaacaga ttttcgcagg
    19201 ttggctgcac atctaaataa ataatttttt caaactgttg tgtaagtact ttgtcaagca
    19261 gcttatgtgg cgaatcgttg ttgagtacta aggcatgatc aaattgtttg ataatttttt
    19321 cctgtactat ttgtttcaat attgaaaaat caatgaccat accagccttc ggattgctat
    19381 tgtcattaaa aggttttccc tttaaggtga cagaaagttt ataggtgtga ccatgaatat
    19441 ttttacatgg accatcgtag ccaaataagg catgagccat gtcaaacaca aaaattttag
    19501 ttacccgaac agtttcattc atcattaatg acatttaaaa tactcaaacg gctgactaca
    19561 ttcgttgcaa taccaaagtg ctttgcatgg tgttgagcca aagcgattca gcattttagt
    19621 ttgtttgctg ttgcaacggg ggcaggtaac cggtttttgc attgcaaata tactatccat
    19681 tccggtttca tcttcagggg gtgcaattcc atgggctttc atcttgtcaa gtcctgactg
    19741 acttatccat tctgtagtcc aggcgggtga tagtatagtt tttatttctg actgaaaccc
    19801 tgcaccattt aatacctgct tgatgtcgtc cacaaaaaca gacatggcag gacagcccga
    19861 ataacttggg gtaattgtga taattgcttt ttcgtttttc cattcaacat tgcggacaat
    19921 gcctaaatca acaatgctga taacaggaat atcaggatca ggaacagtat gcaataatgc
    19981 ccagattaat gattctgttg acttgctgct ttcagtattt tttactgatg acatgacttt
    20041 ttattctacc acttagcatc aggataggct ctctgcaaat attgcatttc gcaaagcatg
    20101 acacctaagc gttctgtatg aataccattg tggccacctg ttatggtgtt gtttacttcc
    20161 ggagtgttaa tatttgccgc agcgaatatt tcatttatgt ttagttgcca cttcgcaaat
    20221 agcgtatccg ttgtaaaaga gataccgtta tcaattaatg cagtgtctgc atcattcatt
    20281 tcaaacatat cttgtgtaaa ccgccacaaa tttgtgacag cttgctgcac acgcatttta
    20341 ctttcatcag ttccgtttcc gaatctgatg agccattcgc tgctgtgtcg caaatgatat
    20401 tttacttcct taatagcttt agcagcaaaa ccagataatg tttcatcatt gctgttcaac
    20461 aacgattcat aaagttgttt tgcaaatgca gaatacaaaa acactttcat catcgtttgt
    20521 gcaaagtcgc cattttcctg ttctaccaaa aggcagttga aatattctct ttcactgcgt
    20581 ctgaaagcaa tatcgtcagc actaagttca tcttccgata atgatgatgc atattcataa
    20641 agcatttcag cctgaccaaa catgtctaat gacatatttg ataaagcaat atcttcttct
    20701 aaaaaaggtc ctctgctgca ccattcagcc aaacgttggc catgaatcat attgctgtcg
    20761 gctaatcgca ggcaatagtt atataatgcg tcttttgttt tcatgattac agattttttg
    20821 ttccttgcgg cattttataa aagtttggtg ttcgatagat tttatcgttt gccggatcaa
    20881 aaaagtcagc agcatcatca gcagtagttg cagctataca ttcagtaggc actacccaaa
    20941 tgctacgtcc ttcatttctg cgtgtgtaaa gatctcttgc attctgcaga gccatttttt
    21001 tgtcagctgc atgcaggctt ccctgatgtt caaaaggtcc accgtttttt gtttgtataa
    21061 atacttccca ttgaggaaac tgattgtcgt ttttcattct ttaatttttt aaattgatat
    21121 ggaagaattt tctttctctc tgcttttttc aaatgcagtt gcacaatcgc gaacccattt
    21181 tccttcatta tgataatgga tatgatgact tatacgctga taattacatg ggccatttcc
    21241 ttttaccaca ctccagaatt catcccagtt aatttcactt gtttcccagt gcatggttgt
    21301 tttgttgtac tttaagtttt tatcaggtat tgtaagtccg atgtggtgtg cctgctcaac
    21361 tgttctgtca ataaaacgct gacgaagtgt gtcattactt tcaactttta ttttccactt
    21421 cattgaatct tttgaatgtg tggattcaga atcatgtggc ccaaacatca tcagtgatgg
    21481 ttcccaccaa cggttcaatg aatcttgtgc catgttgcgt tgtgctgttg ttccattcat
    21541 catctttgca ataatctcat agccttggcg ctgatgaaat ccttcttctt tacagatgcg
    21601 aatcattgca cgcgaataag gcccgtagct gcactttgcc agcatggttt gatttacaat
    21661 agctgctcca tcaaccaacc aaccaattgc acccatgtcg gcccaggtta atgttggata
    21721 attaaaaata cttgaatact ttgcctttcc tgagtgtagc atagcaatca tctctgttct
    21781 gtcaatacca aatgtttcgg ctgctgaata gagataaagc ccatgtccgg cttcgtcctg
    21841 tattttggca agcagaattt tttttgcacg taatgagggc gcacgcaata gccagtttcc
    21901 ctcaggttgc atacctataa tttccgagtg tgcatgttgc gatatctgtc tgaataaagt
    21961 ttgtttatat gcttcaggca tccaatcacg tggttctatt ttttcacctg catcaattct
    22021 ttgctgaaac tgtttttctt tttcttcttc tgtcataact ttaaaaatta taatgttcct
    22081 cgtcttgcct gttctgcttc tatactttca aacaaggctt taaaatttcc tttgccaaaa
    22141 gatttggcac cttttcgctg tataatttca tagaataatg ttggtcggtc ttcaacaggt
    22201 ttggtaaata tttgtaataa ataaccttcc tcatcacggt caaccataat tccccatttt
    22261 ttc
//
LOCUS       DAOOOI010000035        22082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_447_length_22082_cov_14.972942, whole genome shotgun sequence.
ACCESSION   DAOOOI010000035 DAOOOI010000000
VERSION     DAOOOI010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 22082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22082
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_447_length_22082_cov_14.972942"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PKX59_06035"
     CDS             <1..194
                     /locus_tag="PKX59_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90463.1"
                     /translation="CICNMKHSIKKKTINPFNKKELIALSKTWKLKLSQLKDLMGVTG
                     SARLDVLENHLRKTGLWQL"
     gene            complement(198..1508)
                     /locus_tag="PKX59_06040"
     CDS             complement(198..1508)
                     /locus_tag="PKX59_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOZ90464.1"
                     /translation="MNTCIKRGISIICLLLSKSLFAQNLYFPPLTGNNWDTLSPSALN
                     WCQPRIDSLYQFLEDRHTKSFIILKDGKIVLEKYFGTYTADSIHYWASAGKSLTAFLT
                     GIAQEKGFVNIQSPVSQYLGTGWTSAPPAKESLITLRHLLTMTSGLDDSPPPPCDVND
                     SSKACLNYLADAGTRWAYHSGAYKHLQDVISVTSNMSYNAFTTNYLSSKIGMGGLWYD
                     AIFYSKTRGMARFGLLTLSKGIWNNDTILKDTSYISDMSNTSQSLNNSYGYLWWLNGK
                     SSFMIPQLQVVFPGMLLPAAPPDLFAALGKNDQKIYVVPSENLVVVRMGDSAYSSQYA
                     VTVFDNELWTYIDSLSFCPTSLNEINTNQQAWIYPNPANNFLQLDAEASAYFIYNIQG
                     LCCLQGKPDAQRKIDVSMLKEGVYFLKIQTKNNQLRYFKFIRKE"
     gene            1589..5644
                     /locus_tag="PKX59_06045"
     CDS             1589..5644
                     /locus_tag="PKX59_06045"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOZ90465.1"
                     /translation="MNKRFYLLLLICLPMMLWAQNLKPVAQKIFTAKQQQAVFATENL
                     FTVSPDNEQLNRQHAATATKATFVELNKSAIASLMSQKPDFLSLRIPINNNTVQVDLY
                     KANIFTPDFSVTSAGSNGGAVNYTPGLHYQGIINGDNNSIAAISIFDDEVMALISTPA
                     AGNFVIGRLENDRSNLHVIYNDADLTSRPAMECATLADNGTYTPQELQLPANRGMMVN
                     CIRLYWEVNYNIFQGKGSITDAANYVTGIFNQSAIIYTNDNIPVELSEVYVWDVVSPY
                     TGTTSSNYLSQFQTYRTSFNGDLGHLLGYGGGGGIAAGFSGICASNINSSKCYSGINS
                     SYSNFPTYSWSVMVVTHEQGHLMGSRHTHACVWNGNNTAIDGCGPAAGYGYEGSCSGA
                     PIPSGGGTIMSYCHLNAVGINLSLGFGSQPQAVIINKYNSGACLTACIGMACMPPPGM
                     NTSSVNSTSAIFNWTAAASASGYVLRYRVVGTSTWTTATATASPYTATGLTPGSNYEW
                     QVQTVCTGGSSIFTISTTFTTPPITCSVPTGTTTTNINPTYATFSWTAVGGALSYNVR
                     YRLVGGSTWTTANTTSTSYTATSLTANSNYEWQVETVCAGGTTSGFSSSIIFSTPVQP
                     CNTPQNNYTAAITSTSAMLYSSGSAGGVLNIQYKKASNSTWTILNNVSTPYTLTGLQP
                     NTGYQWQVQLVCGLTVSPWSSTVPFTTLCDTPTAVLSPSGTINVCAGPVMLSANTGTG
                     LFYQWLFNGNIISGADLSTYNATVSGSYAVIVTNLNLCSATSASTIVNIGAMTGQITP
                     TGTVSFCAGSNATLNATSGAGYTYQWYRNNVLLSNSSAALSINSSGTYKCVITSGSCT
                     VTTNSVVGNQINNPTPSITTTTPTTFCAPGSVVLTANSFAGVQYQWQKNSVDIVGATA
                     QNYTATSNGSYRVIETANGCSKSKTINVNAASSVSASIYTNDPTTLCNQGSVTMHVNN
                     PIPGYSYQWKNNGNNISGATSTSYTTNVSGNYSCNVSASCGNTNSNAIIVSIGSFNAQ
                     ISPSGNQILCTGGSMLLSASSGIGFTHQWYKNGNAISGATSSTYSATSGGSYTVFISS
                     PCGSGTSPAAVLTENTVSASVSPAGTSTVCAGIPFTFTANSGAGYSYRWYRNNVLLAG
                     TGQSINATSAGQYKVEVSINGSCAVISNIAVLDVINNPAPVITPANATICNGATQVFT
                     ANNFTGVTYQWYRNSVLIPGATAQTYSATLAGQYKVTQTANGCYKSKSAQLTVTSCRE
                     EGEGFIAEAQSNISISPNPFTDQLNIYVPSEKDGNAEIQVLDMLGKVVYRSVITLNQT
                     SEINLHVPAGIYMLSVHTDGKYQVQKIVKSR"
     gene            5757..6194
                     /locus_tag="PKX59_06050"
     CDS             5757..6194
                     /locus_tag="PKX59_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOZ90466.1"
                     /translation="MSGVNKVILVGNLGKDPEMRHIDGNISVAKFPLATSEVYKNKDG
                     QRVTQTEWHNVVLWRQLAETAEKFLKKGSLVYIEGKLRTRNWDDKDGNKKYITEVIGD
                     SMTLLGRKPDENGNPSTGTTERTTSEHPASEAGHSEINDDLPF"
     gene            6277..7587
                     /gene="gldE"
                     /locus_tag="PKX59_06055"
     CDS             6277..7587
                     /gene="gldE"
                     /locus_tag="PKX59_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HOZ90467.1"
                     /translation="MPVVSSLLLSNFSSYSFTGELLVILVLLLLSALFSGSEVAFFSL
                     RPAQLQYLRDEKPTYINKIILHLLDQPKKLLATILISNTCVNIAIIIFSSLIFSNSQL
                     LGDNPILIFFVQVFSVTFLITMFGEVLPKVYAAHNNMRILSFMAYPIYIMGKFLTPLS
                     VPLVKSTSLFDKLLKRKRTNVSLDELTHAIEITSDIESPPEEKRILKGIVNFGNIDVK
                     QIMRPLPDMIAFGLQTPFKELLQRVIDAGYSRVPVYESSIDNIKGVLHIKDLLAHIKK
                     ENFDWPLLIRKTYFVPENKKINDLLQEFQQRKIHMAIIVDEFGATQGLITLEDVLEEI
                     VGEIKDEFDDEEPVYTKIDEQTYVFEAKTSLNEVCRILELDMELFDEIEGEKDTLAGL
                     LLEINHTIPRIGEVITFKNLRFTIESADRRKIKRVKLQITQKQE"
     gene            7584..8150
                     /gene="gldD"
                     /locus_tag="PKX59_06060"
     CDS             7584..8150
                     /gene="gldD"
                     /locus_tag="PKX59_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HOZ90468.1"
                     /translation="MRKLMYILLFGLLTSCSETPAPKPRGFFRIDMPEKKYTLFTDTS
                     CPYRFETPDYSYVLPDTNRFSEPCWKYILYPKFGAQLYLTYKPIRNNLAQMAEDSRGF
                     AYKHTIKANGIDETVINTQNHVYGVVYDIGGNAASNMQFYVTDSFQHFLRGSLYFNTI
                     PNSDSLQPSLEFIRKDVLHLINTLQWSN"
     gene            8525..9901
                     /locus_tag="PKX59_06065"
     CDS             8525..9901
                     /locus_tag="PKX59_06065"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HOZ90469.1"
                     /translation="MPKHSWAEPFKIKMVELLKMTTPQQRKKALQQAGYNTFLLKSED
                     VYIDLLTDSGTSAMSDQQWAGMMLGDEAYAGSKNFYRLEDVVKKYYGFKHLIPTHQGR
                     GAENILSQIMIKKGDVVPGNMYFTTTRLHQELAGATFYDVIIDEAHDSSNLHPFKGNI
                     DINKLDKLVKKFGADKIPYVCVATTVNMAGGQPISMANIKELRKYTAKLGIKVMLDMT
                     RIAENAYFIQQREEGYKRKSIATIVKEICSLTDGATFSGKKDALVNIGGFLALNDDKK
                     FEEASNLVVVYEGLHTYGGLAGRDMEAMATGIIESVSDDHIRARIGQVLYLGDKLNEM
                     NIPIVNPVGGHGIFIDAKKFLPHLKQTQFPAQALAAEIYEDSGVRTMERGIVSAGRNK
                     KTGDHYYPELELVRLTIPRRVYTQAHMDVISESIERVYKNREKIKGLEMIYEPTYLRF
                     FQARFKKR"
     gene            10176..11024
                     /locus_tag="PKX59_06070"
     CDS             10176..11024
                     /locus_tag="PKX59_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90470.1"
                     /translation="MYNKIVGIFTAALTLSLLVTSCSSPPEEKKEPTNVAAKIAPATA
                     PLQNNHLLSVNDAVSLIKNYLDTINNSENAEALRKLPVGGSISAEALKRLTEENPDAN
                     GYMFYGFSDAEPATLFKDVNMLAACKFNFNPDSMVSYETSNETNVYLINDTVKHPYQS
                     LTSEAITNYLSQYTKVIPAAASVHHFAAIKGNMNQYTAFFNENFGDGTVQWPFALFSS
                     DEFLTRLYAATGGENNFGGIRYYFGLDKAAKENRLRIVIFAVNKSGSNVVADNRILER
                     HWPPSE"
     gene            11050..11697
                     /locus_tag="PKX59_06075"
     CDS             11050..11697
                     /locus_tag="PKX59_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90471.1"
                     /translation="MSATCIEVLVALFGLMIFKKAGLLFRLLILLLLYGAATDIFGWT
                     FYKLNRSYVLIAVDIYTLAEALLMVFIIESTGLFNKLKHIFKLIYLLLFICYILAFIM
                     MPLIGDDGAYRGTYSFGYLSTVAVLAAWASLKWIEGAGNKLNSPVFVLLTGVFIYTFC
                     SVFIDSFIGTTTGDAVWWIHDAANITAYLIFAYCFYLIKKTTAIEKSVINLPEKV"
     gene            complement(11675..14014)
                     /locus_tag="PKX59_06080"
     CDS             complement(11675..14014)
                     /locus_tag="PKX59_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOZ90472.1"
                     /translation="MLSYIFRIPNRMYKQLSIAFFLLLSAAQSISQNLPLGSWREHLS
                     YTQAQFVANTPQRVYCATDQAVYFIDKSDNSVQRYSKINGLSDVGIRLMDYSHTENVV
                     FVGYVNSNIDLISSTGIINISDVERRTATGDKAINDVAFNGKFAYLATGFGVLALDLI
                     KAEIKETYVIGTTGSEVPVYSVTTDGTYIYAAIAGGILRGEISNPALNNFATWQMVYP
                     NTFTGITFNKMCWLNGNLAVNFHSSSTDSVVTVNTTNFATGTTLISGGFALRQLKTEN
                     DRLYAVNNYSVNEWDNNFQPLRQIYGNVNYQNTDMFGVATGSGNQLYIADKNNGLVNY
                     TDDNNITFTVPNSPASPNASCVFHNGTTLVTTHGTNRWSDSYGTDGYSTFDGVQWKTW
                     NVKTITNSNVDLNQMLDLQAVVSEPGNPGHFFLGSRINGVFEFDNNVPIKMYNKDNSS
                     LRGAFGNPGQTKIGGLAYDAAGNLWVVNSGVNTPLNMLSKDGVWQSINLQSVIPSGVN
                     IFFGDILCDSYGQVWTTLISNGLIVYDPSQQKSVRISQETGGLNVNDVRCIVEDKEGQ
                     IWLGTSEGIAVIYSPGNATGSTPAKAQKILITIDGITQYLLDKEIVTAIAVDGANRKW
                     IGTNSGGVFLMSADGTKQIQNFNTSNSPLFSNTITSIAIDPVTGEVFVGTDKGLISYV
                     GDATQGTESCSSLKVFPNPVKENYDGPIAVRGVTANGNIKITDIAGNVVYETTAKGGI
                     ATWDGNNFEGKRAQTGVYLVFATDEKGDNTCVTKLFLVN"
     gene            14091..15233
                     /locus_tag="PKX59_06085"
     CDS             14091..15233
                     /locus_tag="PKX59_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOZ90473.1"
                     /translation="MALIAVNTRMLLKDKLDGTGWFAFETLKRIVIQHPEHRFIFFFD
                     RKFNSEFIFSNNIMPVVIYPQARHPWLWYTWFEISLHHAFKKYKPDLFFSPESFICSK
                     ATVPQVAVIHDLNFEHFPQYMPTQVARYYSKWSRIAAQNAQRIGTVSEFSRKDIVSTY
                     EINIEKTDVLYNGIHDMYKPLSVAEQEQVRNQYTAGSRYIIYTGSLHQRKNTANMVRA
                     FAQYKKQYSGELKFVLVSDRSKMEADLQLALSETGIESDLIFTGRLSAKALAQLTASA
                     EAMMYVSFFEGFGIPMIEAMRCGVPVITSNVSCLPEIAGNAALCVDPHSVNAITVALE
                     QVLQHQEVAEQLKQKGKIRQQDFSWDKTAELTWQSLQLVLAEKSKG"
     gene            complement(15244..15702)
                     /locus_tag="PKX59_06090"
     CDS             complement(15244..15702)
                     /locus_tag="PKX59_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HOZ90474.1"
                     /translation="MEAFRLTRAKFANLLSGKGAAIKGARWNSIGVELIYTASNRSLA
                     MAEVAVHFTLATLPADYMMVTINIPDSVSVQEMKEKELPTDWRNFPHPISTQIIGDRF
                     VAENKFCVLRIPSVVTLGDYNLLINPKHRDFVKIKMKSVEKFPFDKRILR"
     gene            complement(15706..16203)
                     /locus_tag="PKX59_06095"
     CDS             complement(15706..16203)
                     /locus_tag="PKX59_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HOZ90475.1"
                     /translation="MSETKLKEKLNKEINTFFHKSRDWDIQFKTTKKITYEDFLSDKI
                     LMIAAIRAGIPYSLFQLIKGFTPFTEYDWADFLDISTKSLQRYHQSSQHYFKPIHSEK
                     IIEMAEVTKVGLEVFEDMDKLKLWLNTPNYALGKIKPIVLLKDSYGKELVMSELIRIN
                     HGILV"
     gene            complement(16328..18460)
                     /locus_tag="PKX59_06100"
     CDS             complement(16328..18460)
                     /locus_tag="PKX59_06100"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOZ90476.1"
                     /translation="MINFLKKYPFVLLVLIAVIGYYQVALFQNTLKWDVTDQYYPWRM
                     FVVQCLRNGILPFWNPYEQFGYPMHADPQSGVWYPVVWLMSLFGPYTLYSVQLEFMLH
                     VIGGGIGMLFLLQRFHVNPFIALINAIAYMFCGLFVGNAQHLTFTIAACWLPFVILAF
                     KNMAEHNHWRFALHFALFGYLLVSGGYPAFAIILFYILLTVTVFLFIRNRQSKHWMKR
                     FLLHSILAVVLLLLCSSGILYSVFQSSDYLARAAGLSFKVASFGPFSPQSLISLMFPL
                     ASARDSEFFGTDISMSSVFVGMLPLAGFILFIIRPKSRSAWLILAATIVMLFASFGSY
                     TPVFGWFYKTFPMLDLFRFPSVYRLFFITGILLLSGIALSAPDAFSRLKLPFATLGGI
                     AAVATIFFWLKNTNEWNFKPWHDAVNNISFTQAIAFQGFIQLLFIVMMLLALLIKDRT
                     KLLMAMITLSAVNVIVAAQLNIPVTVTYNLKTSEVAKEVKEKAVADFPVPEVRPVVLN
                     NDSGGWVSPFWRNLNLFKKQPAWDGYNSFQLKNYVRFVDDESLRQRQISNNWIYFADT
                     VIAYSEEPIIVAPASLHNVCYVPANEKDLLNIQPASGNSISVKTFIPGKIIVEAKTET
                     SSALTLMQQHYHGWKIYDNDKEVKPVVTDYLFMTIPLAQGTHKIEYRYENKPIHNALW
                     LSGVTLIVLIGFSLYAFFSKNKLQALPK"
     gene            18561..18929
                     /locus_tag="PKX59_06105"
     CDS             18561..18929
                     /locus_tag="PKX59_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HOZ90477.1"
                     /translation="MSIKLLTNYLQHHTLIGVKSGIHRKSFTEIWMVTVQGRFFSRSW
                     NKSNDGWYGEILKTGSGQLKLNDTILNVTVKKIKKDDAIQPKIDLAYLSKYTQPENVS
                     YAHGISQPDYHDFTVEFFIE"
     gene            complement(18942..20480)
                     /locus_tag="PKX59_06110"
     CDS             complement(18942..20480)
                     /locus_tag="PKX59_06110"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOZ90478.1"
                     /translation="MATTDDLFKNIISHAKEYGFIFPSSEIYDGLSAVYDYGQYGVEL
                     KKNLRDYWWKAMVQTNPDIVGIDAAIFMHPTTWKASGHVDAFNDPLIDNKDSKKRYRA
                     DVLIEDHVAKIEDKIRKEVEKAAKRFENFDEAMFLSTNARVLEYRQQADKINARFKEA
                     LEAGNLTEVRQIIIDSEIVCPVSGSRNWTEVRQFNLMFSTQLGSVTDDAATIYLRPET
                     AQGIFVNFLNVQKTGRMKIPFGIAQTGKAFRNEIVARQFIFRMREFEQMEMQYFVKPG
                     TEMEWYNHWKEKRFNWHLSLGLGAECYRFHDHLKLAHYANAACDIEFRFPFGFKELEG
                     IHSRTDFDLSNHEKYSGKKLRYFDTEQNTSYVPYVVETSIGLDRLFLAVLSTAYNEET
                     LADGSQRTVLNIPPFLAPIKCAVLPLLKKDGLPEVAQKIFDSLRLSYQCHYDEKDAIG
                     KRYRRHDAIGTPFCITVDHQTLTDNTVTLRHRDSMEQQRIGINDLKAILDKELALENI
                     LAKL"
     gene            complement(20567..20977)
                     /locus_tag="PKX59_06115"
     CDS             complement(20567..20977)
                     /locus_tag="PKX59_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ90479.1"
                     /translation="MKSSSPSRLQHVLLIDDNEIDNFINERIITSSGFSEVCTVKTSA
                     DEAIEYLNIISKDSKVPLPQVIFLDLNMPVKDGFAFLDEFNALAENIRQSCRIIVLSS
                     SISPDDINRASANPYVIRYLNKPLAEKYLEAIDL"
     gene            complement(21002..21074)
                     /locus_tag="PKX59_06120"
     tRNA            complement(21002..21074)
                     /locus_tag="PKX59_06120"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21039..21041),aa:Phe,seq:gaa)
     gene            21284..21802
                     /locus_tag="PKX59_06125"
     CDS             21284..21802
                     /locus_tag="PKX59_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90480.1"
                     /translation="MLIQNKKILIATLAVLFFIGISFHGYCITSEPEHSHQHHKAEIG
                     VGQSAVYFTKDKTLNYGLHLHLIRNIKHSGFGVGIEYERIFDRHGHNMLGIVGSYSPA
                     HQVELSITPGILFEDSEPGHADASIHIEAGYHFDIKNIHLGPIVECALDRHDVHFSLG
                     VHVGIGLGKIRE"
BASE COUNT         7044 a         4256 c         4178 g         6604 t
ORIGIN      
        1 aatgtatatg taatatgaaa cattccatta aaaagaaaac catcaatccc ttcaacaaaa
       61 aagagttgat tgctttatca aaaacgtgga agctaaaact ttcgcagcta aaagacctga
      121 tgggcgttac aggaagcgca cgcttagatg ttttggaaaa tcaccttcgc aaaacaggcc
      181 tgtggcaatt gtaatcttta ttcttttctt ataaatttaa aataccgcag ctgattattc
      241 tttgtctgta tttttaaaaa atagactcct tcttttagca tcgaaacatc aatttttctt
      301 tgtgcatcag gcttgccttg caaacaacac aggccttgta tgttgtaaat aaaatatgct
      361 gatgcttctg catcaagctg taaaaaatta tttgcaggat taggataaat ccatgcctgt
      421 tggtttgtat tgatttcgtt gagcgatgta gggcagaatg ataaactgtc aatataggtc
      481 cacagttcat tatcaaaaac agtaactgca tactggctgc tgtaagcaga gtcgcccatg
      541 cgaacaacaa ctaaattctc agaaggcaca acataaattt tctgatcgtt ttttccaaga
      601 gcagcaaaca aatcaggtgg agctgcgggc aacaacattc ccggaaaaac tacctgcaac
      661 tgcggtatca taaacgatga cttgccgttg agccaccaca gataaccata ggagttgttt
      721 aaactctgcg atgtgttgct catatcagaa atgtatgatg tgtcttttaa gatggtatcg
      781 ttattccata ttcctttgct caatgtaagt aaaccaaaac gcgccatacc tctggtctta
      841 ctataaaata ttgcatcgta ccaaagtccg cccataccaa ttttagagct taaataattt
      901 gtggtaaaag cattgtagga catattactt gttacgctga tgacatcctg aagatgttta
      961 tatgcaccgg aatgataggc ccatcgtgtg ccggcatcgg caaggtagtt gagacatgcc
     1021 tttgatgaat cattaacatc gcatggtgga ggtggcgaat cgtctaatcc gctggtcata
     1081 gtcagtagat gtcgtagtgt gataagactt tctttggcag gtggtgcact tgtccatcct
     1141 gtgccgagat attgcgaaac gggcgactga atgtttacaa aacctttttc ctgagctatg
     1201 cctgtaagaa aagcagtaag gctttttcct gcagaagccc aatagtgaat agagtcggca
     1261 gtataagtac caaaatattt ttcgagaaca atttttccgt ctttaagaat gataaaactt
     1321 ttagtatgtc tgtcttcaag aaactgatac agtgagtcaa tgcgcggctg acaccagttt
     1381 aaagctgatg gagaaagtgt gtcccaattg tttcctgtta atggtgggaa atagagattc
     1441 tgtgcaaaaa gtgattttga taacagcagg caaattatcg atatgcctct tttaatacaa
     1501 gtattcatct gactgcaaca tttattacaa agatattttg aatttaagaa aactataata
     1561 tatttggaat cttataatcc tcaaatttat gaacaaacga ttttacttat tattattgat
     1621 ttgcctacct atgatgttgt gggcgcagaa tcttaaacct gtagcacaaa aaatatttac
     1681 tgccaaacaa cagcaggcag tatttgcaac agaaaacctt tttactgtca gtccggataa
     1741 tgaacaactg aacagacaac atgcagctac tgctaccaaa gcaacttttg ttgagttgaa
     1801 caaatcggct attgcctctt taatgagtca gaaacctgat ttcttatcgt tgcgtattcc
     1861 tattaacaac aacaccgttc aggttgactt gtataaagca aacattttta cccctgattt
     1921 tagtgttaca tctgcaggca gcaatggtgg tgcagtgaac tatactcccg gtttacatta
     1981 tcagggaatt ataaatggtg ataacaattc catagcagcc ataagcattt ttgatgatga
     2041 agtgatggca ttgatttcta ctccggcagc agggaatttt gtgattggcc gtttggaaaa
     2101 cgatcgttcg aacttacatg taatttataa cgatgcagat ctgacttcac gacctgcaat
     2161 ggaatgtgca acacttgccg acaatggaac ctacacacca caggaattgc agttgcctgc
     2221 caacagaggt atgatggtaa actgtatcag attgtattgg gaagttaact acaatatttt
     2281 tcaaggtaaa ggaagcatca cagatgctgc aaactatgta acagggattt ttaatcagag
     2341 tgcaataatc tataccaacg acaacattcc tgttgagttg agcgaagttt atgtttggga
     2401 tgttgtgagt ccatatacag gaacaacatc ttcaaattac ctgagtcaat tccagacata
     2461 ccgtaccagc tttaatggcg acttaggtca cttgcttggc tatggtggtg gtggtggaat
     2521 tgctgcgggc tttagtggca tttgtgcatc aaacatcaac tcaagtaaat gttatagtgg
     2581 catcaactca tcatacagca atttcccaac ttactcatgg agtgtaatgg ttgttacaca
     2641 cgaacaaggt cacttaatgg gttcaagaca tacacatgcc tgcgtgtgga atggcaacaa
     2701 cactgccatt gatggttgcg gtcctgctgc aggttatggt tatgaaggca gttgcagtgg
     2761 cgcaccaata ccgtcaggtg gtggaactat tatgagctat tgtcatttga atgctgtggg
     2821 tattaatctt tctttaggat ttggttcgca gccacaagcg gtaatcatta acaaatacaa
     2881 cagtggtgca tgtttgacag cctgcatagg aatggcatgt atgcctcctc cgggaatgaa
     2941 cacatcttct gtgaacagca catctgcaat ttttaactgg acagcagcag caagtgcttc
     3001 aggctatgta ttgcgttatc gtgttgttgg tacatcaaca tggactaccg caacagcaac
     3061 tgcatcacca tacactgcaa caggactgac accgggaagc aactacgagt ggcaagtgca
     3121 gacagtttgt acaggaggct cttctatatt tactatttca acaacattta ctacgcctcc
     3181 gattacatgc agtgttccaa caggaacaac aacaaccaat ataaatccaa cttatgcaac
     3241 atttagctgg acagctgttg gtggtgcatt aagctataat gttcgttatc gtttggttgg
     3301 tggttcaaca tggacaactg caaatactac atcaacaagt tacactgcaa catctttgac
     3361 agcaaatagc aattacgaat ggcaggtaga aaccgtttgt gcaggtggaa caacgtcagg
     3421 attttcttct tcaataattt tttcaactcc ggttcaaccg tgcaatacac ctcaaaacaa
     3481 ttataccgct gccattacaa gtacatcagc tatgttgtat tcttcaggtt cagcaggtgg
     3541 cgtattaaac attcagtata aaaaagcaag taattcaacc tggacaattt taaataatgt
     3601 atcaactcca tatacattaa caggtttaca acccaacacc ggttatcaat ggcaggtaca
     3661 gcttgtttgc ggactaactg tttctccatg gagcagtacg gttccattta ctactttatg
     3721 cgatacacca acagcagtat tatctccttc aggaactatc aatgtttgtg caggaccggt
     3781 aatgctttct gcaaacacag gaacaggtct gttttaccaa tggttattta acggaaacat
     3841 tatttccggt gctgatcttt caacttacaa tgccaccgta tccggcagct acgcagtaat
     3901 tgtaaccaac ttaaatttat gtagcgctac atcagcatct accattgtaa acattggtgc
     3961 aatgacagga caaattactc caacaggaac agtaagtttt tgtgcaggct ctaatgccac
     4021 attaaatgcc acatcaggtg caggatatac ataccaatgg tacagaaaca atgtgctgct
     4081 ttcaaattca agtgcggctt taagcatcaa ctcatcaggt acttataagt gtgtcattac
     4141 atcagggtcg tgtacggtaa caacaaacag tgttgtaggt aatcagatta acaatcctac
     4201 accttcaatc acaactacta caccaacaac attctgtgca ccgggttctg tggtgcttac
     4261 agcaaacagt tttgccggtg tacagtatca gtggcagaaa aacagtgtgg acattgttgg
     4321 agcaacagct cagaattata ctgcaacatc taatggatcg tacagagtta ttgaaacagc
     4381 caatggctgc tctaaatcaa aaactattaa tgtaaatgct gcatcttctg tttctgcatc
     4441 tatctacacc aacgacccaa ctacactctg taatcaaggt tcggtaacaa tgcatgtaaa
     4501 caatccaatt ccgggatata gctatcagtg gaaaaataat ggtaacaaca tttcaggcgc
     4561 tacttcaaca agttatacta ccaacgtttc aggaaactat tcatgcaacg ttagtgcaag
     4621 ctgtggcaac actaacagca atgctattat tgtaagcatc ggaagtttca atgcacagat
     4681 aagtccttcg ggcaatcaga ttctttgtac cggtggttct atgttgttaa gtgcatcatc
     4741 aggaataggt tttacacatc agtggtataa aaacggtaat gcaatttctg gagcaacaag
     4801 ctcaacatat agtgcaactt caggaggaag ttatacagtg tttatttctt cgccttgcgg
     4861 cagtggaaca tcacctgctg ctgtgttaac tgagaatact gtctctgcct ctgtaagtcc
     4921 tgcaggtaca tcaacagttt gtgcaggtat tcctttcaca tttactgcca atagcggtgc
     4981 cggttattcc tatcgctggt atagaaacaa tgtgttgctg gcaggaacgg gacaaagtat
     5041 taatgctact tctgcaggac aatataaagt tgaagtttca atcaacggtt catgtgctgt
     5101 aatcagtaac attgctgttt tagatgtcat taacaatcct gctccggtta ttactccagc
     5161 aaatgcaact atttgtaacg gagcaacaca ggttttcaca gcaaataatt ttacaggggt
     5221 gacttaccaa tggtacagaa acagtgtatt aattccgggg gcaacagcac aaacctattc
     5281 tgcaacatta gcaggacaat acaaggttac acaaactgca aatggatgtt acaaatcaaa
     5341 atctgctcaa ctcacagtaa cttcttgtcg cgaagaaggt gaaggcttta ttgctgaagc
     5401 tcaaagcaac atcagcatat cgccaaatcc atttacagac caactcaata tttatgttcc
     5461 atcagaaaaa gatggcaatg ctgaaattca ggttttggat atgttaggta aggttgtata
     5521 tcgttctgta attaccttaa atcaaacatc agaaattaac ctgcatgttc cggcaggaat
     5581 ttacatgctg tctgttcaca ctgatggaaa atatcaggtg cagaaaattg tgaaatcgag
     5641 atagtaaagt cgttcttaaa aaaagacccc gctgctgcgg ggttttttta tgaccaaatt
     5701 gttatatttg tatgcacaaa caaacacatt cgttacgcag ttaaacaaca gcaatcatgt
     5761 caggagtaaa taaagtaatt ttagtaggca acttaggaaa agatccggaa atgcgtcata
     5821 tagatggtaa catcagtgta gctaaatttc cattggcaac atcagaagta tataagaata
     5881 aagacgggca aagagtaaca caaacagaat ggcataatgt ggttttgtgg cgacaacttg
     5941 cagagactgc agaaaaattc ctgaagaaag gtagcttggt atatattgaa ggaaaactac
     6001 gcacacgcaa ctgggatgat aaagacggca ataaaaaata cataacagaa gtcattggcg
     6061 acagcatgac acttcttggt cgcaagcccg atgaaaacgg aaatccttca acaggcacta
     6121 ctgagagaac aacatctgaa catcctgcat ctgaggccgg tcattcagaa ataaatgatg
     6181 atttaccatt ttaagcgcgt tgcatagttt ttaataccca actaagcaac atcatcctat
     6241 atttgcagga aattatattg tactcacccc ctcagcatgc ctgtcgtcag tagcttgtta
     6301 cttagcaatt tttcatccta ttcatttacc ggtgagctat tagttattct tgtgttgctt
     6361 ctactttctg cacttttttc aggcagcgaa gttgcctttt tctcactacg ccctgcacaa
     6421 cttcagtatc tgagagatga aaaaccaaca tacatcaata aaataattct gcatttactc
     6481 gatcagccta aaaagttgtt ggctacaatt ttaatttcca atacctgtgt aaacattgca
     6541 ataattattt tttcttctct catcttttca aactcgcagt tgttgggcga taatcctata
     6601 ctcatttttt ttgtacaggt ttttagtgtt acgtttctca tcaccatgtt tggcgaagtg
     6661 ctgccaaaag tttatgcagc gcataacaac atgcgtattc tttcctttat ggcttacccg
     6721 atttatatca tgggaaaatt tttaacgcca cttagtgttc cattggtaaa atcaacttct
     6781 cttttcgata aacttcttaa gcgcaaacgc accaacgtaa gcttggatga attgacacat
     6841 gccattgaaa ttaccagcga tattgaatcg ccaccggaag aaaaacgtat tctcaaaggc
     6901 attgtaaatt ttggaaatat tgatgtcaaa caaatcatgc gaccgttgcc ggacatgatt
     6961 gcttttggct tgcaaacacc atttaaagag ttgttgcaac gtgtaattga tgccggctat
     7021 tcaagagtgc cagtatatga atcttctatt gacaacatca aaggagtatt gcacattaaa
     7081 gacttattgg cacacataaa aaaagaaaat ttcgactggc cactacttat ccgtaaaaca
     7141 tattttgttc ctgaaaacaa aaagataaat gatttattgc aggagtttca acaacgcaaa
     7201 atacacatgg ctataattgt tgatgagttt ggtgcaacgc aagggcttat tacattagaa
     7261 gatgtacttg aagaaatagt aggtgaaata aaagacgagt ttgacgatga agagcctgtt
     7321 tacaccaaga ttgatgagca aacctatgtg ttcgaggcta aaacatcact gaatgaagtt
     7381 tgccgtattc ttgaattgga tatggaattg tttgatgaaa tagaaggtga aaaagacacc
     7441 cttgcaggac tgttgttgga aatcaatcac accataccac gtattggcga agtaataact
     7501 ttcaagaacc tgcgttttac tattgaatca gccgacagac gaaaaataaa acgtgttaaa
     7561 ctgcagatta cacaaaaaca ggaatgagaa agttgatgta tatcttactt tttggtttac
     7621 tgacatcgtg cagcgaaaca cctgcaccaa agccacgcgg attttttcgt atagatatgc
     7681 ctgagaagaa atatacttta tttacagaca cctcctgtcc atatcgtttt gaaacacctg
     7741 actatagtta tgttttgccc gataccaatc gcttttcaga accctgttgg aaatatattc
     7801 tctatccaaa atttggtgca cagctttatc ttacttataa gcctataaga aacaaccttg
     7861 cccaaatggc cgaagattcc agaggctttg cctacaagca taccatcaaa gccaatggca
     7921 ttgacgaaac cgtaatcaac acacaaaatc atgtttacgg tgtggtatat gatattggtg
     7981 gcaatgcagc aagcaatatg caattttatg ttaccgacag ctttcagcat tttttaagag
     8041 gatcattgta ttttaatacc ataccaaatt cagattcgtt gcagccatca ttggagttta
     8101 tacgcaaaga tgttttacac ctgataaaca ctttacaatg gagcaattaa aaaaatatcg
     8161 ctatacatta attttattgg cagtagcgtt aatgctgtgt caatcatctt gcaggctttt
     8221 caaaaaaaaa tgtgattgcc caaagtggag cagtcatacc aaaaaatcac agacggttta
     8281 attgcgaaat ttatttctgc ttttattcgg attttcattt tcagaacaaa gaattttagc
     8341 tgcattttta atcaaactga ttttaatcat attcatatca ccttaaattg aagtttttat
     8401 aggattttac caatgaaagt caatttttag gtcttagcta agttttacta ttgcaaacga
     8461 atttttaatt gtttcaaata aattcaaaag caattcataa acacatcaaa aaaaataaat
     8521 tattatgcca aaacattcat gggcagaacc ctttaaaata aaaatggttg agttgttgaa
     8581 aatgacaaca ccacaacaac gaaaaaaagc attacagcaa gcaggttaca acacgtttct
     8641 acttaaatct gaagatgtat atattgactt gttgacagac agtggaactt ctgccatgag
     8701 cgatcagcaa tgggcaggaa tgatgcttgg tgacgaagct tatgccggca gtaaaaattt
     8761 ttatcgctta gaagatgtcg taaaaaaata ttacggattt aaacatctta tccctacaca
     8821 tcagggccgt ggtgcagaaa atattctgtc gcagataatg attaaaaaag gtgatgtggt
     8881 tccgggcaac atgtatttta ctacaacacg ccttcaccag gaacttgcag gtgcaacatt
     8941 ctatgatgtc attattgacg aggcacatga cagctctaac ctgcatccat ttaaaggtaa
     9001 tattgacatc aacaaattag ataagttagt taaaaaattt ggtgccgata aaattcctta
     9061 tgtctgtgta gcaactacag ttaacatggc cggtggtcag ccaatttcta tggctaatat
     9121 taaagagctt agaaaatata ctgcaaagct tggcataaag gttatgttgg atatgacacg
     9181 cattgcagag aatgcctatt tcatacaaca gcgtgaagaa ggttataaaa gaaaaagtat
     9241 tgcaaccatc gtaaaagaaa tctgcagcct gactgatggt gcaacattta gcggaaagaa
     9301 agatgcattg gttaacatag gaggctttct ggctttgaac gatgataaaa agtttgaaga
     9361 agcaagtaat cttgttgttg tatatgaagg actgcacact tatggtggat tagccgggcg
     9421 tgatatggaa gcaatggcaa caggcattat tgaatctgtt agtgatgatc atatcagggc
     9481 gcgtatcgga caggtgctat atttaggtga taagctcaat gaaatgaata ttccaattgt
     9541 aaatcctgtg ggtggacatg gaatttttat tgatgctaaa aagtttttgc cgcatcttaa
     9601 acagacacag tttcccgcac aggcattagc tgctgaaatt tacgaagaca gcggtgtacg
     9661 taccatggaa agaggaattg tatctgcagg cagaaataaa aaaactggcg accactatta
     9721 tcccgaatta gagttggtgc gactgactat tccacgcagg gtttatacac aggcacacat
     9781 ggatgtgatt tcagaatcaa tagagcgtgt ttataaaaac cgagaaaaaa taaaaggatt
     9841 agaaatgatt tatgaaccta cctatctgcg attctttcag gcaaggttca aaaaaagata
     9901 agtgtttttt gtaattattg attactaaag gaggctaaga gcctcctttt ttgttctgtt
     9961 acaactgcag aaaacagtct gctttttgca tcttgtaccg tcataaagca ttttgtacaa
    10021 ttgttgttgg aataagtttt ttcagaagta tatttgaaaa atattaattt aaaacgattt
    10081 tttaataaac taaaataata accaacgacc gataatttaa ttagtcagac agaaaataaa
    10141 ccgctcttta ttgctgacta tctaaaacca aatatatgta taacaaaatt gtcggaattt
    10201 ttacagcagc cctaacactt tccctgcttg taacctcgtg tagtagccca cccgaagaaa
    10261 aaaaagaacc gactaatgtt gctgcaaaaa tagcaccagc aacagcgcca ttacaaaata
    10321 atcacttgct aagtgtgaat gatgctgtgt cgttaatcaa aaattattta gacacaatta
    10381 acaattccga aaatgccgaa gctttaagaa aactacctgt tggcggatcc ataagtgccg
    10441 aggccttaaa aagattgaca gaagaaaacc ctgatgccaa tggctacatg ttttatggtt
    10501 tttctgatgc tgaacctgca acgttattta aagatgtaaa tatgcttgca gcttgtaaat
    10561 ttaactttaa tcctgatagc atggtaagtt atgaaacaag taacgaaacc aatgtttatc
    10621 tgataaacga tactgtaaaa cacccttatc aatcgctgac atccgaagcc atcacaaact
    10681 atctgtcgca gtacaccaag gtaattcctg cagctgcatc tgtccatcat tttgcagcca
    10741 ttaaaggaaa catgaatcaa tacaccgctt tctttaatga aaactttgga gatggaactg
    10801 tgcaatggcc ttttgccttg ttttcatctg atgaattttt aacccggctt tatgcagcaa
    10861 caggtggtga aaataatttt ggtggcatac gttattattt tggattagat aaagcagcaa
    10921 aagagaatag actcagaatt gttatttttg cagttaacaa atcgggttcc aatgtagttg
    10981 cggataatag aattcttgaa cgccattggc ccccatcaga atgataaaat ggatcctttc
    11041 cttactcgaa tgtccgcaac ctgcattgaa gtgctggtgg cattgttcgg gttaatgatt
    11101 tttaaaaaag ccggactgct gttccggctt ttgattttat tgcttttgta tggcgcagca
    11161 accgatattt ttggctggac attttataaa ctaaaccgtt cctatgtatt gattgcagtg
    11221 gacatttata cgctggcaga agcactactc atggttttta taattgaatc aacaggatta
    11281 tttaacaaac taaagcatat ttttaagcta atttacctgt tgctttttat ttgctatata
    11341 ctggcattta tcatgatgcc attaataggt gatgatggtg cttatagagg aacttactcc
    11401 tttggctatt tgtcaaccgt tgctgtgttg gcagcatggg catcgctaaa atggattgaa
    11461 ggtgcaggaa ataaactcaa cagccctgtt tttgttttac ttacaggtgt ttttatttac
    11521 actttctgca gtgtgtttat tgattctttt attggcacaa caacaggtga tgctgtctgg
    11581 tggattcatg atgcagccaa cataactgct tatctgattt ttgcttattg cttttacctg
    11641 attaagaaaa ccacagcaat agaaaagtct gtaattaatt taccagaaaa agtttagtaa
    11701 cacaggtatt gtcgcctttt tcatcggtag caaaaaccaa ataaactccg gtttgcgcac
    11761 gcttgccttc aaagttgttg ccatcccatg ttgcaattcc accttttgct gttgtttcat
    11821 aaacaacgtt tcctgctata tcagtaattt taatattgcc atttgctgtt acaccacgca
    11881 ctgcaatagg gccatcgtaa ttctctttca ccggattggg aaatactttt aaactagaac
    11941 acgactctgt tccctgtgtg gcatcaccaa cataagaaat caatccctta tcagtgccaa
    12001 caaaaacttc ccccgtaaca ggatcaatag caattgatgt aatggtgttt gaaaacaaag
    12061 gactgtttga tgtattaaag ttctgaatct gcttggtccc atctgccgac attaaaaata
    12121 cacctccact attggtgcca atccattttc tgttggctcc atcaacagct attgctgtaa
    12181 caatttcttt gtctaaaaga tactgcgtaa taccgtcaat cgttatcaga attttttgtg
    12241 cttttgccgg agtgcttcct gttgcattgc cgggcgaata aataactgca ataccttctg
    12301 atgtcccaag ccatatctga ccttctttat cttcaacaat acaacgcaca tcattaacat
    12361 ttaatccacc tgtctcctgc gaaattctaa ctgatttttg ttgcgaagga tcataaacaa
    12421 tcaatccatt gctgattaac gttgtccaca cctgaccata agagtcgcat aaaatatctc
    12481 caaaaaatat atttactcct gatggaataa ctgactgaag attgatagac tgccaaacac
    12541 catctttact caacatattc aatggtgtgt ttactccgga atttactacc catagatttc
    12601 ctgctgcatc ataagctaaa cctccaattt ttgtttgccc cggatttcca aaagctcctc
    12661 taagactgct gttgtctttg ttgtacatct ttataggaac attattgtcg aactcaaaaa
    12721 caccattgat gcgcgagcca agaaaaaagt gtccgggatt tccgggctca ctgactacag
    12781 cttgtaaatc gagcatttga ttgaggtcaa catttgagtt ggttattgtt tttacattcc
    12841 atgttttcca ctgcacacca tcaaatgttg aataaccatc agtaccataa ctgtcgctcc
    12901 agcggttagt gccatgcgta gttaccaatg ttgtaccatt atgaaaaaca cagcttgcat
    12961 tgggcgaggc agggctgttt ggaactgtaa acgttatgtt gttgtcgtct gtatagttaa
    13021 ccaaaccatt atttttatca gcaatgtata gttgattgcc actacctgta gctacaccaa
    13081 acatgtctgt gttctgatag ttgacattac cgtaaatctg gcgcaatggt tgaaaattat
    13141 tatcccattc attaacagaa taattattca ctgcataaag cctgtcattt tcggttttta
    13201 actgtcgcag tgcaaagcca ccactaatta atgttgttcc tgttgcaaaa tttgttgtgt
    13261 taacggtgac tacactatct gtagaagaag aatgaaaatt aacagccaga ttaccattaa
    13321 gccagcacat cttattaaat gttattccgg taaaagtgtt tggataaacc atttgccatg
    13381 tagcaaaatt attgagtgcg ggattgctta tttctcctct aaggattccg cctgcaatgg
    13441 cagcataaat ataggtgcca tcagtggtaa ctgaatacac aggaacttca cttcctgtgg
    13501 ttccgataac gtaggtttct tttatttcag ctttaattaa atcgagtgct aaaactccaa
    13561 agcctgttgc cagatatgca aactttccgt taaatgctac atcattaata gccttgtcac
    13621 cggtagctgt tcttctttca acatcagaaa tattaataat gcctgtactt gatatgaggt
    13681 caatattgga atttacatag cctacaaaca ccacattttc tgtatggctg taatccatca
    13741 gccttatacc tacatctgaa agtccgttaa tttttgaata acgttgtacg ctgttgtccg
    13801 acttatcaat aaaatataca gcctgatcgg tggcgcaata aacgcgttgt ggtgtgttag
    13861 ccacaaactg tgcctgcgta taggaaaggt gctcgcgcca tgagcctaat ggtagatttt
    13921 gcgaaatgga ttgtgctgca gagagcaaca gaaaaaaggc aatggacaac tgtttgtaca
    13981 tacggttcgg gatacggaat atataactca acatgaggga atttatttta tggcctgcta
    14041 tgagtttcta tttaaaatac ttttgcacta caaaaatata aataatcgca atggcgctga
    14101 tagcggtaaa caccagaatg ttgcttaaag acaagttaga tggaacagga tggtttgcat
    14161 tcgagaccct gaagcgtatt gtcattcagc atcccgaaca tcgttttatc ttttttttcg
    14221 accgtaaatt taattctgag tttattttca gcaataatat tatgccggta gtcatttacc
    14281 cgcaggcacg tcatccgtgg ctttggtaca cctggtttga aataagcctt caccacgctt
    14341 ttaaaaaata taaaccggat ctattcttca gtcccgagag ttttatttgc agtaaggcta
    14401 ctgtaccaca ggtagcagta attcacgatt taaactttga acattttcca caatatatgc
    14461 caacacaagt tgctcgttac tatagtaagt ggtcgcgcat agctgcacaa aatgcacaaa
    14521 gaataggtac tgtgtctgag ttttcgcgaa aggatattgt gagcacctat gaaattaaca
    14581 ttgaaaaaac cgatgtgttg tataatggta ttcatgatat gtacaagcca ttatcagtag
    14641 cagaacaaga gcaagtacgc aatcaatata ctgcaggaag ccgctatata atttacaccg
    14701 gctcattgca tcagcgaaaa aacacagcta acatggtgcg tgcttttgca cagtataaaa
    14761 agcaatatag cggagaatta aaatttgtat tagtgtccga tcgaagtaaa atggaagctg
    14821 atttgcaact tgcactttct gaaacaggaa ttgagagcga cttgattttt acaggaaggc
    14881 tttctgcaaa agcattggca caactaactg catcggcaga ggcaatgatg tatgtttcgt
    14941 tttttgaagg atttggtatt ccgatgattg aagctatgcg ctgtggtgta cctgtaatca
    15001 cttccaatgt aagttgcctt cctgaaattg caggcaatgc agctctctgt gttgacccac
    15061 acagcgtaaa tgccattacc gttgctttgg aacaagtatt acaacatcag gaagttgccg
    15121 aacagctaaa acaaaaaggc aaaatcagac aacaggattt ttcttgggat aaaactgctg
    15181 aactaacgtg gcaaagcctg caacttgttc ttgctgaaaa aagcaaagga taagagttag
    15241 tctttatctg agtattctct tatcaaaagg gaatttttct acagatttca tttttatttt
    15301 aacaaagtca cgatgctttg ggttgatgag taaattatag tctcccagag taaccactga
    15361 gggaattcgt aaaacacaaa acttattttc agcaacaaat ctatcaccaa taatttgagt
    15421 tgatattgga tgcgggaagt ttcgccaatc ggttggcagt tctttttctt tcatttcctg
    15481 aacagaaacg ctatccggaa tatttattgt taccatcata taatctgcag gtaatgttgc
    15541 taaagtaaaa tgtactgcaa cttctgccat agctaaagag cggtttgaag ctgtatagat
    15601 caattctaca ccaatagaat tccaccttgc gcctttaata gcagccccct ttccggataa
    15661 taaatttgca aacttggctc tggtcagtct gaatgcctcc atagactaaa caagaatacc
    15721 atggttgatt cttatcaatt ctgacattac taattccttt ccataagagt ctttcaataa
    15781 tacaataggt tttatttttc ctaaggcgta atttggagta tttaaccaaa gtttcagttt
    15841 atccatatcc tcaaaaactt caagtccgac tttagttact tctgccattt ctattatttt
    15901 ttctgagtgt attggtttga agtaatgttg agatgattga tgatatcgct gtaatgactt
    15961 ggtagatata tcaagaaaat cagcccaatc atattctgta aaaggtgtaa aaccctttat
    16021 taattgaaac aacgaataag gtatgccggc tcttattgcc gcaatcatta aaatcttatc
    16081 tgaaagaaaa tcttcatacg ttattttctt cgttgtcttg aactgtatat cccaatctct
    16141 cgacttatga aagaaggtgt ttatctcctt attaagtttc tcttttaatt ttgtttctga
    16201 cataacaaga catttgtcag caaatataag acatttttct gtaactgtca attaattcta
    16261 tttgcggtaa tgagaaatta acaaacaggt ttgtgaaaca ataaatttta aataatattc
    16321 aataacatta ctttggcaaa gcctgcaact tgttcttgct gaaaaaagca taaagtgaaa
    16381 agcctatcag tacaattagc gtaactcccg aaagccacaa agcattatgt atcggtttgt
    16441 tttcataacg gtattctatc ttatgagtac cttgtgctaa aggaatcgtc ataaataaat
    16501 aatcggtaac gacaggttta acttctttgt cattatcata aattttccat ccatgatagt
    16561 gctgctgcat cagtgttaat gcagaagatg tttctgtttt tgcttcaaca ataatttttc
    16621 ccggaataaa tgtctttaca gaaatgctat tgcctgatgc tggttgaata ttcaacaagt
    16681 ctttttcgtt tgcaggcaca taacaaacat tatgcagcga tgcaggtgcc acaatgattg
    16741 gctcttcaga ataagcaata acagtgtcgg caaaataaat ccagttgttg cttatctgtc
    16801 tttgcctgag cgattcatca tcaacaaagc gtacataatt tttcagctgg aaagaattat
    16861 agccatccca tgccggttgt tttttaaaca ggttaagatt tctccaaaaa ggacttaccc
    16921 acccaccgga gtcgttgttg agtacaacag gccgcacttc aggaacagga aaatctgcaa
    16981 cagctttttc ttttacttct tttgctactt cagaagtttt aagattgtaa gtcaccgtta
    17041 caggaatgtt taattgtgct gcaacaatta cattaactgc agacaatgtt atcattgcca
    17101 tcagaagctt tgttctatct ttaattaata aagccagcaa catcatcaca ataaaaagca
    17161 actgaataaa accctgaaaa gctattgcct gagtaaacga aatgttgttt acagcatcat
    17221 gccagggttt aaaattccat tcattagtat tctttaacca gaaaaaaatt gtagctactg
    17281 cggcaatgcc acctaaggtg gcaaagggta acttaaggcg gctgaatgca tcgggagcag
    17341 atagtgcaat gcccgaaagc aacaatattc cggtaataaa aaacaatcta tatacagagg
    17401 ggaatcggaa taaatcaagc atgggaaaag ttttgtaaaa ccagccaaaa accggggtgt
    17461 agcttccaaa tgatgcaaag agcatgacga ttgtcgctgc aagaataagc catgcagagc
    17521 gcgatttggg tctgatgata aagagtataa accctgccaa cggcaacata ccaacaaaca
    17581 cagaggacat actgatatct gtaccaaaaa attcactgtc gcgagcagaa gccaatggaa
    17641 acattagcga tatgagcgac tgtggtgaaa agggaccaaa cgaggctact ttgaatgaaa
    17701 gacctgctgc ccgtgcaaga taatccgaac tttgaaaaac actgtacaaa atacctgatg
    17761 aacacagtaa taacaacaca acagcaagta tgctgtgtaa taaaaaacgt ttcatccaat
    17821 gtttgctctg acggttgcgg ataaaaagaa atactgttac tgtaagcaga atataaaata
    17881 aaataatggc aaatgcagga tatccaccag ataccaacaa gtaaccaaac aatgcaaagt
    17941 gcaaagcaaa acgccaatga ttatgttctg ccatgttttt aaaagcaagt atgacaaaag
    18001 gcagccaaca ggcagcaatg gtaaaggtta gatgctgtgc attgcccaca aacaaaccgc
    18061 aaaacatata agcaattgca ttaatgagtg caataaaagg gttgacatga aaacgctgta
    18121 ataaaaacaa cataccaatt cccccaccta tgacatgcaa cataaattcc aattgcacac
    18181 tgtaaagtgt atatggacca aacaacgaca tgagccagac aacaggatac cacacaccac
    18241 tttgcgggtc ggcatgcatg gggtagccaa attgctcgta tggattccag aaaggcaaaa
    18301 taccatttcg cagacactgc accacaaaca tgcgccacgg ataatactgg tctgtgacat
    18361 cccatttcaa tgtgttttga aaaagtgcaa cctgatagta accaataacg gctatcagca
    18421 ccaataacac aaaaggatat ttctttaaaa aatttatcac gggcgtaaaa atagaaggaa
    18481 ttagtgatgt tgaaaatata aattgataat agatgctttt ttccgattta aaaatttacg
    18541 tttgccatca aacaccgtaa atgtctatca aacttcttac aaattacctg caacatcaca
    18601 cattaatagg agttaaaagc ggtattcaca gaaaatcatt tacagaaatt tggatggtaa
    18661 ctgttcaagg acgtttcttt tctcgaagct ggaacaaaag taatgacgga tggtatggcg
    18721 aaattttaaa aaccggttca ggtcagttga aactcaacga caccatcctg aacgttactg
    18781 tcaaaaaaat taaaaaagat gatgccattc agcctaaaat tgatttggct tatctttcaa
    18841 aatacacaca gcccgaaaat gtttcttatg cgcatggcat ttcacaaccc gattatcatg
    18901 attttacagt agagtttttt atagaatgaa aaaaatccgg cttacagttt agccagaata
    18961 ttttctaatg caagttcttt atctaaaata gctttcaaat cattaattcc aatacgctgt
    19021 tgctccatac tgtcgcggtg acggagtgta accgtattat cagttaatgt ctgatggtca
    19081 actgtaatac agaaaggtgt tccaatggca tcgtgtctgc ggtaacgttt accaattgca
    19141 tctttttcat cgtaatgaca ctgatatgaa agtcttagtg aatcaaatat tttctgagcc
    19201 acttcaggca gtccgtcttt tttcaataga ggtaaaacag cgcatttaat gggtgctaaa
    19261 aatggaggaa tgtttaacac tgtacgttgc gaaccatcgg caagtgtctc ttcattgtag
    19321 gctgtgctga gtactgcaag aaaaagtctg tcgaggccta tacttgtttc caccacataa
    19381 ggaacatacg atgtgttttg ctctgtatcg aagtagcgta attttttacc tgaatatttt
    19441 tcgtgattag ataagtcaaa atctgtgcgc gagtgaatac cttcaagttc tttgaatcca
    19501 aaaggaaaac gaaactcaat atcacaggcg gcattggcat agtgtgccag cttcaggtgg
    19561 tcgtgaaagc ggtagcattc tgcaccaaga ccaagagaaa gatgccagtt aaaacgtttc
    19621 tctttccagt ggttatacca ttccatttcg gtgccgggct ttacgaagta ctgcatttcc
    19681 atctgctcaa attcacgcat acggaaaata aactgtcgtg ccacaatttc atttctgaaa
    19741 gcttttcctg tttgtgctat accaaaagga attttcatcc tacccgtttt ctggacgttc
    19801 aaaaagttta caaaaattcc ctgtgctgtt tcggggcgca gataaatggt tgcagcatca
    19861 tctgtaacac ttccaagctg tgtagaaaac atcagattaa actgccgcac ctctgtccag
    19921 ttgcgcgagc cgcttacagg acaaacaatt tctgagtcta taattatctg tctaacctct
    19981 gtaagattac ctgcttctaa ggcttcttta aaacgtgcat ttatcttgtc ggcctgctga
    20041 cggtattcaa gcacccttgc attggtagaa agaaacatgg cctcatcaaa attttcgaat
    20101 cgcttggctg ccttttcaac ttctttgcga attttatctt caatttttgc aacatgatct
    20161 tctatcagca catcggcacg atagcgtttt ttagaatctt tattgtcaat caaaggatca
    20221 ttaaatgcat ctacgtgtcc ggatgctttc catgttgtag gatgcataaa aatggctgca
    20281 tctattccaa caatatcggg attagtctgc accatagctt tccaccagta gtcgcgcaga
    20341 ttttttttca gctccacacc atattggcca taatcataaa cggcactcag cccgtcatag
    20401 atttcgctcg aaggaaaaat gaatccatac tccttggcat gtgatataat atttttaaat
    20461 aaatcgtctg ttgtagccat aggcagcaaa ggtacgtgga atagattatt gtaaaacttt
    20521 atcggataat tttaaagagt aatgcggaat acggaaacga aaaatcttaa aggtctatag
    20581 cttcaagata tttttctgct aatggcttgt tcagatatcg gataacataa ggattggcag
    20641 aggcgcggtt aatatcatcc ggactgatgg atgatgacaa cacaatgatg cggcatgact
    20701 gacggatatt ttctgcaaga gcattaaact catcaagaaa agcaaaaccg tcttttacgg
    20761 gcatgttcag gtcaagaaaa attacctgtg gcaatggtac cttactgtct ttggatatga
    20821 tgttcagata ttcaatggct tcatcggcag aggtctttac ggtacatacc tcgctgaacc
    20881 ctgaagaggt gataatacgc tcgttgatga aattatctat ttcattatca tcaatcaaca
    20941 gaacgtgttg caatcgagaa ggagaagatg atttcattga atcttttcct gaaaatgtta
    21001 gtggtgacag gtggaatcga accaccgaca caaggatttt cagtcctttg ctctaccaac
    21061 tgagctatgt caccgttaat gagtgcgcaa aagtaaaaaa ataatgaata gaaaaaactt
    21121 tttgttgggt ttattgcccc tgtctcagac catttttcaa aaacaggaca cttattttct
    21181 ttagctttga acaaactata attttatttc agacaaggtc aaaagcgctc agggctttaa
    21241 tgaaaaccat tcttctcaaa aatcattttt attacacatt aaaatgctaa ttcaaaacaa
    21301 aaaaatcctt attgccaccc ttgcagtttt attttttatt ggaatttctt ttcacggata
    21361 ttgcatcacc tctgagccag aacacagcca tcagcatcat aaagctgaaa taggtgttgg
    21421 ccaatcggca gtttatttta caaaggacaa aacattaaac tatggtctgc acctccacct
    21481 gatacgcaac attaaacatt ccggttttgg tgtaggtata gagtacgaaa gaatttttga
    21541 ccgccatgga cataacatgc tgggaattgt cggcagctat agtccggcac atcaggtgga
    21601 actaagtatt acaccgggca ttttatttga agactccgaa cccggacatg ccgatgcatc
    21661 catacacata gaagcaggct atcatttcga cataaaaaat attcaccttg ggccaatagt
    21721 tgaatgtgca ctcgacagac acgatgtaca ctttagcctt ggtgtgcatg taggtattgg
    21781 gttagggaaa ataagggagt gatggtttgg aggaaatttt atctgttgtt cttattaata
    21841 gtttttatat ttacataaaa aactattaat cctcttcgca ctctttgcgt gaaagaaaac
    21901 tacacttgct acaaatacgc ctttgcgctc tttgcgtgaa agaaaaagtc tcttgctaag
    21961 actcaaagaa aaaaaatacg cagaggccgc taagaaaatt cattctttgc gaactttgcg
    22021 tgaaatactt tttcagaata gcgggtatgg ctccatgaaa gttgatataa gtgattttaa
    22081 ac
//
LOCUS       DAOOOI010000036        21550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_467_length_21550_cov_29.154873, whole genome shotgun sequence.
ACCESSION   DAOOOI010000036 DAOOOI010000000
VERSION     DAOOOI010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 21550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21550
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_467_length_21550_cov_29.154873"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="PKX59_06130"
     CDS             <1..175
                     /locus_tag="PKX59_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOZ90481.1"
                     /translation="ALLIKVLSGRTPEEIMSAELKFIDAIGLRQHVAVTRANGLANMI
                     KQIKAYALAHKER"
     gene            complement(186..1070)
                     /locus_tag="PKX59_06135"
     CDS             complement(186..1070)
                     /locus_tag="PKX59_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOZ90482.1"
                     /translation="MNISIPTIFKGHGSAVYCLLPKNKNAFYSGSGDHHLIEWNIETP
                     DKGTVVAILPAGIYSLISENNILIAGCGNGEVHCINTQNASALSKAHLHSSYVFDLCF
                     YNQHLYTAGGDGQVVKTDLQLNKLESIHLGNFKIRKLLPHPDGQHILTACGDGTVRIL
                     NVNTLKEEQVIIAHTAGFSVNALCLTPDNKFLLTGSRDARLNVFDVQQNFNLIENIAA
                     HNYAIYDIAFNPSGKIMATASRDKKIKIWNNDLTFITRIEEYPVGHKNSVNTLCWLSD
                     TELITSGDDRNIMLWHLN"
     gene            complement(1073..2305)
                     /locus_tag="PKX59_06140"
     CDS             complement(1073..2305)
                     /locus_tag="PKX59_06140"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOZ90483.1"
                     /translation="MYSEQSHKFHGQLFKYATESVVVTNSKGIIVTVNPSALKLFGYT
                     ENEIIGQKIEKLMASRFEASHQLHREGFNKNPHNRAMGIGLDLHAMRKDGSEFPVEIS
                     LSPFSLNDESYVMAFIIDITGRRKVQAQMLEQKNQLEEITRELKQTNERLEAKVLDRT
                     KVLEEALNEIEKSRKELSEALAKEKELNELKSRFLSMASHEFRTPLTTILSSASLITA
                     YAEKDQQDKRIRHVERIKSAVNNLNDILSDFLSLSKIEEGKTKTDFEVFNLKNIVSEI
                     INDLATIKKPGQAINYVHTGKNEVLLDPRLIRNIVINILSNAIKFSDEGKLIEIKSNI
                     GENTTVLEFKDQGIGISKEDQQHLFERFFRGSNATNIQGTGLGLNIVARYVELMNGVI
                     ETESELNKGTAFKITIPC"
     gene            2350..2940
                     /locus_tag="PKX59_06145"
     CDS             2350..2940
                     /locus_tag="PKX59_06145"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOZ90484.1"
                     /translation="MIKSKWINIFVLMKRGDRVIFIFFMLWISTTVKGQSVYSCTNGE
                     VFFRSEAKLELIDAKSQKLEAIVDFQKKTFEFLIPINSFKGFNAELQREHFLDRYMES
                     DKFPVATFKGKIIEDVDLSKPTEITIRAKGKLTIHGIEQERIIKSKISVQGNVVSILS
                     VFSVPLKDHKIKVPRVVQEKIANEVLVQVKATLKRK"
     gene            2937..5810
                     /locus_tag="PKX59_06150"
     CDS             2937..5810
                     /locus_tag="PKX59_06150"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOZ90485.1"
                     /translation="MKRIVFILFCIVQTVNGQTPFFKQIALNRDSPNLTVNTLLFCKN
                     GFLWAGTSEGLYRSDGNDHHLLATSDTIKRNVTALFEDNTGVIWIGTKEGEIGFVKDD
                     TLRIIKAFSLKPSVAITSINADTDAIKWLTTAGNGVYYLLDSKFYHIGIEEGLSDDYC
                     YTSAPDKSGNMWIGTDQGLSLCTSKNGKKVLQKLQVADGLCDNIVKKIVIEGSKMFLA
                     TQENGLCIYDFEQNGFLKQKAETVWNYGSIDDIILNKTTNEIWVATESGGLLYFRNPE
                     EKTYSLKQQSAKLFSSVYSVAIDHQNNVWVATDRGLFRSHGQWLTFLEQYAGADINNV
                     HEVAFLDSNQMVVSIKNKIMLISFNTSTIKEWTILPSEKNIDVSGLYIDKEKYIWIGT
                     LGNGVFRINPLKSEIRKIEIPFDYSSILYINGNEKNIWLGTFGGAALIEVEQLNTDNL
                     KYKVRIFSHESPLGNYYVYCVYRDVKGRFWFGTDQNGIVLLDDNKFVAPFSGKFNGRT
                     VYSITGDNNDAIWFSVADEGIVCLNGDSFKLYNRSNGIRDLNITSIAVLDDNSIAIVN
                     KRGIDILNHKSGSVMYLGTENNLDNLNSDINSVAKDSIGRIWFGTVKGMVVLDPKLLK
                     QNTVPELSKLNMIQPIFKNRSGITPVFRHNENTVTFEYAATWFTNPGRILYQYKLDGL
                     NADWIATKDIKQVFPNLPPGKYVFRIRTSIDPQFTFNNESSFSFEIKKPFWQEDWFYF
                     SSLLLAILLVYLFIKVRDARMKKLQQLKNDSIRFQFETLRNQVNPHFLFNSFNTLIDI
                     IEHDKEHAVDYVENLSAFFRNIVTYKDKETIKLSEEIKIAKAFYFIQQKRFGSNLQLL
                     IDEHSLYDEKYVVPMVLQLLLENAIKHNEISKEKNLEVHVAFTNDRIIISNNTNPKVS
                     KDESTGTGLRNIIARYRLLTDKTVQIESSQDYFVVSLPLINHQ"
     gene            5810..6571
                     /locus_tag="PKX59_06155"
     CDS             5810..6571
                     /locus_tag="PKX59_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOZ90486.1"
                     /translation="MKIVIFEDEEPASRRLIKLLNEINANVEIVSVIDSVRSGKEFMS
                     QNKNFDLIISDIRLADGLSFEIFKSYPVKLPVIFSTAYDEYAIEAFRNNGIDYLLKPV
                     KREELKSAIQKYENWFSDSTSGVNYDALLQTLLQEKKGFQKRIVIRFADTIKTVEIED
                     AAYFFTENKINNLCTFGGESFIVDHNLDELELMLDPSVFFRINRQFIVNFKAIQKMNV
                     VSKSRVKLTLHPTTIQDTIVSTERSGVFKQWLAGK"
     gene            complement(6581..8437)
                     /locus_tag="PKX59_06160"
     CDS             complement(6581..8437)
                     /locus_tag="PKX59_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ90487.1"
                     /translation="MKNYFKILRYVKPYWGYALLNASCNILSVLFSFFSLTLIAPFLD
                     LLFLKDDNFYLEKMAAGKPEFHLSTKAIVDSFYYYLTEMITDPNRGKQYALMFICIAV
                     AVMFFLKNLFRYLGQFFISPIRNGVVKDLRADVHKKMMRLPLSWFSNERKGDIITRVT
                     SDVHEVEWSVMSSLEVVFRDPFNIFLFLGMMIFISAKLTVFVFILLPIVGFLIGRIGK
                     SLKRTSARSKEKLGLLISIIEETLSGLRIIKAFNAEKFMAEKFAKVNERYNTIAIRAL
                     RKTDLSSPLSEFLGTVTMMIVMYFGGMLVLGKDASLSASAFITYIALFSQIIPPAKSF
                     TQAFYNIQKGLASADRIFAVLEAEESIKDQQSAETIKSFNYKIEYQNISFSYRKGDEG
                     YVLKNVSFTIEKGKTVALVGQSGSGKTTLADLLPRYYDVDGGTLLIDGTDIRKLQLHS
                     LRELLGVVSQEPILFNDTIYNNIAFGMNHATEKMVEEAASVANAHEFITQMPEGYQTN
                     IGDRGSKLSGGQRQRISIARAVLKNPPILILDEATSALDTESEKLVQDALTSLMKNRT
                     VLVIAHRLSTIQHADEIIVLQKGEIVQRGTHVQLANVEGVYKKLYELQGLSS"
     gene            8530..9513
                     /locus_tag="PKX59_06165"
     CDS             8530..9513
                     /locus_tag="PKX59_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOZ90488.1"
                     /translation="MQHDPYVILGISPSATPEEIKRAYRKKAFELHPDRNSDQHAEQL
                     FRELNEAYALLTDADKRRNYDKQYQNYSRSNTDQFDHYFEMHVSSNNVKVCEEFELIF
                     TYTGEGRFMQKPDLRDFYITGKPFVSFRDVYRSGKTARETTIRYVLAAKRVGSFTINR
                     AAIKLFGKKFITATQYIQVISGECFFSPDKPADGRPLQVSLWYNAEKGGTNRKYLVNT
                     KHTIYIPRSNYAQVYHTIGRVLKLIFAFWGFFVAVKISRSGIIGFAIGSAYGAIMTYL
                     LYYFVKVKPVFMSERKYSLVISYLQNGYQPYPPHARNLISRALMFINKLFL"
     gene            complement(9542..10321)
                     /locus_tag="PKX59_06170"
     CDS             complement(9542..10321)
                     /locus_tag="PKX59_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOZ90489.1"
                     /translation="MSNKNYRIICWNVNGIRAIWKKGFPEWLSKEMPDVLCLQETKAQ
                     PEQLDDEIKDFNGYKSHFFSAQKKGYSGVAIYTKREPLKVRYGLDDDAFDIEGRCIEM
                     EFEDFVLYTAYFPNGGRGPERVKYKLDFYESLFQRAENMRKKNKNIVICGDYNTAHQE
                     IDLARPKENSKTTGFLPIEREWIDKIIGMGYVDIFREFNQESGQYTYWDQITRARDRN
                     VGWRIDYYMISTELRKYAKSAPIHMNVMGSDHCPIELNLSF"
     gene            complement(10339..11391)
                     /gene="thiL"
                     /locus_tag="PKX59_06175"
     CDS             complement(10339..11391)
                     /gene="thiL"
                     /locus_tag="PKX59_06175"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOZ90490.1"
                     /translation="MFENANNRTELSSLGEFGLIRHLENAVEIKNKETLKGIGDDAAV
                     ICLDSEENVQLITTDMLLEGVHFDLVYCPLKHLGYKSVVVNLSDIYAMNGIPKQIVVG
                     IGLSNRFSLEAIEELYSGMLLACKHYGVDLIGGDTTSSRSGLVISITAIGTAKKSDVV
                     YRNGAQENDLICVSGDLGAAYLGLQLLNREKKVFLDSPGVQPDFQGNDYLLERQLKPE
                     ARKEIIEILKKTGVKPTAMIDISDGLASEIMHICTQSDKGCTIYEAKIPIDEKTYNLA
                     REFHLDPTVCAMNGGEDYELLFTINQKDFEKINSEADISIIGHITAKEEGYYMLDKSD
                     RKVALKAQGWDALKSN"
     gene            11530..11856
                     /locus_tag="PKX59_06180"
     CDS             11530..11856
                     /locus_tag="PKX59_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HOZ90491.1"
                     /translation="MITVTEKAKSKALSLMQAENKPADAFIRVGVEGGGCSGLTYMLV
                     FDTELKPEDKVFEDKGIKIVCDRKSFLYLVGTELDYTDGLNGKGFQFNNPNASRTCGC
                     GESFAV"
     gene            11928..13373
                     /gene="sufB"
                     /locus_tag="PKX59_06185"
     CDS             11928..13373
                     /gene="sufB"
                     /locus_tag="PKX59_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOZ90492.1"
                     /translation="MSEDLKIIDEVTSGEYKWGFVSNIESENAPKGLSEDIVRFISAK
                     KKEPEWMLEFRLKAFRHWQKMQEPQWANVNYPKVDFQDIIYYSAPKPKKQLNSLDELD
                     PEIKATFDKLGISLLEQKRLAGVAVDAVIDSVSVKTTFRQELSKLGIIFSSFSEAVQE
                     HPELVKKYMGSVVPYTDNFYAALNSAVFSDGSFCYIPKGVRCPMELSTYFRINSAGTG
                     QFERTLIIADEGAYVSYLEGCTAPKRDENQLHAAVVEIYAHTDAQVKYSTVQNWYPGD
                     KDGRGGIFNFVTKRGLCAGERSKISWTQVETGSAVTWKYPSVILKGDYSTGEFYSVAL
                     TNNYQQADTGTKMIHIGKHTRSTIISKGISGGRSNNSYRGLVRIGKQAEKARNFSQCD
                     SLLLGDKCGAHTFPYLECANPTAKVEHEATTSKVGEDQIFYCNQRGISTEDAIGLIVN
                     GYCKEVFNQLPMEFAVEAQKLLAVSLEGSVG"
     gene            13419..14171
                     /gene="sufC"
                     /locus_tag="PKX59_06190"
     CDS             13419..14171
                     /gene="sufC"
                     /locus_tag="PKX59_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOZ90493.1"
                     /translation="MLNIKNLHASVEGREILNGLNLQVNPGEVHAIMGPNGSGKSTLA
                     SVLAGREAYEVNDGEILYHGENLIELSPEERARKGIFLAFQYPIEIPGVSNANFLKTA
                     VNEVRKSKGMDVLDSKDFLKLMKDKMQLVKMDKSMTQRSVNEGFSGGEKKRNEIFQMA
                     MLEPELCVLDETDSGLDIDALRIVSDGINALRDGKRSFIVITHYQRLLDYIVPDFVHV
                     LYKGKIVKSGPKELALELEEKGYDWIKEEVEI"
     gene            14183..15487
                     /gene="sufD"
                     /locus_tag="PKX59_06195"
     CDS             14183..15487
                     /gene="sufD"
                     /locus_tag="PKX59_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOZ90494.1"
                     /translation="MNSSVDTLSVTNETSIAEDNIPVDGLQKLRLAALNKFLEKGFPD
                     SKHEEWKYLNLKPISETKYNHRVVSSPLKASDLEQFYDAGNDTVNLVFINGRYCSEFS
                     GHQSHKHPFSIYSLESREAELYLETLRNSEDEPFHALNTAFHQGGIMIHVPDDCRMPM
                     TIHLLYVNDSQNEAVAAHPKCIVVAGKNSAFQVIATYHSLNNFPSLTNTVTEFLIGEN
                     AVVEFDIKQNENSNANHISRVYARVQRKAVFDIFTVTMGGALVRNNLEIILSEPNACA
                     HLNGLTVADGYQVVDNHTVVDHASPNCESNQLYKNILDDHSQGVFNGKIFVRKDAQKT
                     NAFQSSKNVLLSNTAVMNAKPQLEIFADDVKCSHGATTGQLDEDALFYFRSRGIGERD
                     ARALLNYAFASDVIEKINNDALKSNLLKLLSQKLNTAVDFEE"
     gene            15731..16996
                     /locus_tag="PKX59_06200"
     CDS             15731..16996
                     /locus_tag="PKX59_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011743985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOZ90495.1"
                     /translation="MSELPDNSVHLAITSPPYWQLKDYGTENQIGFHDSYENYINNLN
                     LVWKECYRVLHDGCRLCVNIGDQFARAVYYGRYKVIPIREEIIKFCENIGFDYMGAII
                     WQKVTTSNTTGGGVQMGSYPYPRNGILKLDYEFILVFKKLGDSPKPTKEQKELSKMSA
                     EEWNTFFAGHWNFAGARQNNHIAMYPEELPRRLIKMFSFVGETILDPFVGSGTTALAA
                     KNTERNSVGFEINPAFIQIIKEKLEVHQLDLNATTYEFVKQKKINLDFEKEIQKLPYI
                     FKDPHILDKKIDVKKLQFGSKIDKDSKTKREEMFTVKEVLSPEKIRLNNNMTVKLIGV
                     KEDPNINGKATSFLQEKIKGKRVFLKYDNVKHDSENNLLCYLYLENKTFINAHLIKSG
                     FVKVDIGVEFKYKEKFQSLFNEVHAQADK"
     gene            16977..17675
                     /locus_tag="PKX59_06205"
     CDS             16977..17675
                     /locus_tag="PKX59_06205"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MjaI family restriction endonuclease"
                     /protein_id="HOZ90496.1"
                     /translation="MPKPINKRFSKEFGKKEKVLNYTTQTYQLSRPNKVGAVMALIRE
                     CQPKTIKEWEKWYFENAKTNEKTPVKITKKSLRELGERLHIKIKEIVIPEWTEAFSQL
                     TLQDCIDYIYNLTINRTYDGFIREKSVIEDNLAKKFPKVKFEESDPELDHAGDIDYLG
                     WVEKRAFGIQIKPVTSKANFGNYSPSERMKASFSSFTKKYGGKVFIVYSIDDKIKNTE
                     VIKQITAEIKKLAK"
     gene            complement(17707..18978)
                     /locus_tag="PKX59_06210"
     CDS             complement(17707..18978)
                     /locus_tag="PKX59_06210"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOZ90497.1"
                     /translation="MNIPDLKIPRVVIVGCGFGGLQLAKSLRKARVQIVMFDTNNYHT
                     FQPLLYQVATSGLEPDSIAYPIRKIFKKQKNFLFRWGKVENIIPEKNIIQTTVGELAY
                     DYLVIATGTTTNYFGNADIAQHAMPMKSVIEAINLRSLILQNFEQALLTQSLKEQEAL
                     MTFTVVGGGPTGVELAGALCELKKHVLPNDYPELDFRKMRVLLIEGTNSVLQAMSKEN
                     QHRALKYLDELGCEVWLETQVSSYNGEEVVFKNSKVLPSKNLLWAAGIKGVTISGLNK
                     DAINNRGRYVVDEYCKVKGCENIYAIGDVASMSTADYVNGHPQVAPVAIQMAQLLAEN
                     IELELRQKSLKKFKYFDKGTMATVGRNRAVTESFNIRFGGFAGWLVWMALHLMLLVGF
                     RNKVVVFINWLWNYINYDRNIRLIIPPFKRK"
     gene            complement(19056..19520)
                     /locus_tag="PKX59_06215"
     CDS             complement(19056..19520)
                     /locus_tag="PKX59_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOZ90498.1"
                     /translation="MSEPIKRNEALKPISRDHHHALLLGWKIRQGIKNKIPAERIKKY
                     VDWFWSSYLENHFELEEKNLYPVLGAEHASIKKAMQHHEKLRNLFQSKEQTIQQLDEL
                     QNELDRHIRFEERELFNEIQNAASAKQLQDMAAVHNEAFHDNYSDEFWVIKK"
     gene            complement(19517..20242)
                     /gene="ric"
                     /locus_tag="PKX59_06220"
     CDS             complement(19517..20242)
                     /gene="ric"
                     /locus_tag="PKX59_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HOZ90499.1"
                     /translation="MQITKDTIVGDLVAKDYRTATVFKQNGIDFCCNGNRTLEEVCKQ
                     KEMSVTNIIGELTQASQQASAAQTDFNSWPLDLLADYIEKKHHRYVEEKIQEIKPYLD
                     KICKVHGDHHPELYEINQLFNDSAGELAMHMKKEELILFPAVRRMVKSQMQKTTPEKP
                     QFGPINNPIQMMMHEHNNEGERFRQIEALSNKYNPPQDACNTYRVTFAMLKEFEEDLH
                     LHIHLENNILFPKSLELEKAISS"
     gene            complement(20254..21024)
                     /locus_tag="PKX59_06225"
     CDS             complement(20254..21024)
                     /locus_tag="PKX59_06225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ90500.1"
                     /translation="MDTKDIQKAHGHWILAKMGKKVLRPGGKELTKKLIDALHFNDSD
                     NVVEFAPGMGFTASLTLAHKLHSYVGVDADEDIVRMLSKRLQNSQASFILGNASQTNL
                     ESDSKDKVYGEAMLTMHADHRKSEIIREAHRILKKGGWYAIHELGLTDVDDTLKSKIQ
                     RDLALSIKVNARPLTENEWKHLLEQEGFAVKKVMTNGMHLLEMNRIIDDEGLMGATKI
                     GWNILTHPKARKRILEMRKVFKQYQTHMNAIAIVAEKL"
     gene            complement(21194..>21550)
                     /locus_tag="PKX59_06230"
     CDS             complement(21194..>21550)
                     /locus_tag="PKX59_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HOZ90501.1"
                     /translation="LKLFEEKYLESFIALAKKVKGNTIEDKVMQMGNCSDALKTKLKE
                     FDHMYNVAWPIVHLETAQHYLDLKGENKAATGGSEWKKYLHPKYQQRKFFPSLWTKEE
                     IANWGSNEFQKTTNNK"
BASE COUNT         6979 a         3693 c         3941 g         6937 t
ORIGIN      
        1 tgccttgctg ataaaagtat tgagcggacg cacaccggaa gagataatgt ctgcagagtt
       61 gaaatttatt gatgccatag gtctgcgtca acatgttgct gtcacacgtg ccaatggttt
      121 ggccaatatg ataaagcaga ttaaagctta tgcattagca cataaagagc gttaataatt
      181 tacgattagt taagatgcca tagcattata tttctgtcat caccgctagt gattagctct
      241 gtgtccgaaa gccaacaaag cgtattcaca gaatttttat gaccaacagg atactcttca
      301 atacgagtaa taaaagttaa atcattattc caaattttta tttttttatc gcgactggct
      361 gttgccatta tttttcctga agggttaaat gcaatatcat aaatagcata attatgagca
      421 gcaatatttt cgattagatt aaaattttgt tgtacatcaa atacatttag tcttgcatca
      481 cgtgagcctg tcagaagaaa tttattgtca ggtgtgaggc aaagtgcatt tacagaaaaa
      541 cctgccgtgt gagcaataat aacttgttct tcttttaaag tatttacatt tagtattctg
      601 acagtaccat caccacaagc tgtaagaata tgttgcccat caggatgagg taagagtttt
      661 ctgattttaa aattacccaa gtgaatggat tctagtttat ttaactgtaa gtccgttttc
      721 acaacttgtc catcaccacc tgctgtatag agatgttggt tgtaaaaaca taaatcaaag
      781 acataactac tatgcaaatg tgcttttgat aaagcagaag cattttgtgt attaatacaa
      841 tgtacttcgc cattgccaca gccggcaatc agaatgttgt tttctgaaat taaactgtaa
      901 ataccggcag gaagaatagc aactacagtg cctttatcag gagtttcaat gttccattct
      961 atcagatgat ggtcgccact gccggagtaa aatgcatttt tattttttgg tagcaggcaa
     1021 tatacagcac tgccatgccc tttaaaaatt gtaggaatag aaatattcat tattagcagg
     1081 ggatagtaat cttaaaagca gttcctttat ttaattcact ttcggtctca attacaccat
     1141 tcatcagctc aacatatcgg gcaacaatat ttaaaccaag tcctgtacct tgtatgttag
     1201 tagcattcga ccctcggaag aagcgttcaa acaaatgctg ctgatcttcc ttgctgatac
     1261 ctattccctg atctttaaat tcaagtacag ttgtattttc accaatgttt gattttattt
     1321 caatgagttt accttcatca gaaaatttaa tggcattgga aagaatatta ataacaatat
     1381 tccgaatcag ccgtgggtcg agtaacactt cattttttcc tgtatgcaca taatttatgg
     1441 cttgcccggg ttttttgatg gtagccaaat cattgatgat ctcacttaca atatttttca
     1501 gattgaacac ttcaaaatca gtcttagttt ttccttcttc aattttactc agagaaagaa
     1561 agtcgcttaa aatatcattc agattgttga cagcagattt tattctttca acatgtctga
     1621 ttcgtttatc ctgttgatct ttttcggcat aggcagtaat gagtgatgca gatgaaagaa
     1681 tagttgtaag cggtgtacga aactcatgcg atgccataga caaaaacctt gacttgagtt
     1741 cattcaattc tttttctttt gcaagcgctt cgcttaattc ctttcttgac ttttcaattt
     1801 cattcaatgc ttcctccaac acttttgttc ggtccagaac tttagcctct agtctttcat
     1861 tggtttgttt taactcacga gtaatttctt caagttgatt tttttgttca agcatttgtg
     1921 cctgtacttt tcttcggcct gttatgtcaa tgataaatgc cataacatac gattcgtcat
     1981 tcaaagaaaa tggactcaga ctaatttcaa ccggaaattc actgccatct tttcgcatag
     2041 catgtaaatc gaggccgata cccatagcac gattatgcgg atttttatta aatccttctc
     2101 tgtgtaattg atgggatgct tcaaaacggg atgccatcaa tttttctatt ttctgaccaa
     2161 taatttcatt ctcagtgtat ccaaagagct ttaatgcaga tggatttact gtaacaataa
     2221 taccttttga attggtaaca acaacgcttt ctgttgcata tttaaaaagt tgaccgtgaa
     2281 atttgtgact ttgttcagaa tacataatca atcatcaaaa acacaaagtt agagcattaa
     2341 aagcaaacaa tgataaaatc caaatggatt aatatttttg ttttaatgaa acgaggtgat
     2401 agagtaatat ttatattttt catgctttgg atttctacta ctgtcaaagg gcaatcagtt
     2461 tattcctgta ccaatggcga agtgtttttc cgcagcgaag ccaaacttga gttgattgat
     2521 gccaaatctc aaaagttgga agccattgta gattttcaaa agaaaacatt tgaattttta
     2581 atccccatca actcttttaa aggatttaat gctgaattgc agagagaaca ctttcttgac
     2641 cgttatatgg agagtgataa atttcctgta gcaacattta aaggtaagat aattgaagat
     2701 gttgatctct ctaaaccaac tgaaataaca ataagagcaa agggcaaact aacaatacat
     2761 ggaatagagc aggaaaggat tatcaagtca aaaatttctg ttcagggaaa tgttgtaagt
     2821 attttatcgg tttttagtgt tccgttaaaa gatcataaga taaaagtacc gcgtgttgtt
     2881 caggaaaaga ttgccaatga agtgttagta caggtaaaag ccacattaaa acgcaaatga
     2941 aaagaatagt ttttattctg ttttgtattg tacaaactgt gaatgggcaa accccatttt
     3001 ttaaacagat tgcattaaac agggattcac ctaatttaac agtcaatact ttattatttt
     3061 gtaagaatgg atttctttgg gctggcactt cagaaggtct ttaccgtagc gatggaaacg
     3121 atcatcattt acttgcaaca agtgatacca ttaagagaaa tgtaactgca ttatttgaag
     3181 acaataccgg tgtaatttgg ataggcacta aggagggtga aattggcttt gttaaagatg
     3241 atacattaag aattatcaaa gcattttctt taaagccatc agtagccata acatctatta
     3301 atgcagatac ggatgctatt aagtggttaa caacagcagg taatggagtt tattatttgt
     3361 tggatagcaa gttttatcat atcggtattg aagaaggatt aagtgatgac tactgctaca
     3421 cttctgcccc ggataaatca ggtaatatgt ggattggaac agatcaggga ttgtcgcttt
     3481 gtacatcaaa aaatggaaaa aaagttcttc aaaagcttca ggttgcggat ggattatgtg
     3541 ataatatagt taagaaaata gttatagaag gttcaaaaat gtttcttgca acacaagaaa
     3601 acggattatg tatttatgat tttgagcaga atggttttct gaaacaaaag gcggagacgg
     3661 tttggaatta tggatcaata gatgatatta ttctaaataa gacaactaat gaaatttggg
     3721 ttgcaacaga atccggtgga cttttatatt ttcggaatcc tgaggagaag acctattctt
     3781 taaaacaaca gtcagcaaaa ttatttagct ctgtatattc agtagcaata gatcatcaga
     3841 ataatgtttg ggttgcaaca gaccgtggtt tatttcgatc acatggacaa tggctgacat
     3901 ttttagaaca atacgctggt gctgatatta ataatgtaca tgaggttgca ttcctagata
     3961 gcaatcaaat ggttgtgagt attaaaaata aaataatgct catatcattt aatacatcta
     4021 ctataaaaga atggacaatt ctaccttcag aaaaaaatat tgatgtttcc ggtttatata
     4081 ttgacaaaga gaaatatata tggataggta ctttaggtaa cggtgttttc aggataaatc
     4141 ctttaaagag tgaaatcagg aaaatagaaa ttccatttga ttatagcagt attttatata
     4201 taaacggtaa tgaaaaaaat atctggcttg gaacatttgg gggtgctgct ttaattgaag
     4261 tagaacaatt aaacactgat aatcttaaat ataaagtcag aattttttct catgaatcac
     4321 cactaggaaa ttattatgtt tattgtgttt atcgtgatgt aaaagggcgg ttttggtttg
     4381 gtaccgatca gaatggaata gtgctgttag atgacaataa atttgttgct cctttttcag
     4441 ggaaatttaa tggcagaaca gtatattcaa taacaggtga taataatgat gcaatatggt
     4501 ttagcgtagc tgatgaagga attgtttgcc ttaatggaga cagttttaaa ttatataacc
     4561 gctccaatgg aatccgcgat ttgaacataa ccagtattgc agtgcttgat gataacagca
     4621 ttgcaatagt taataaaaga ggcattgata tattgaatca taagtcaggt tcagttatgt
     4681 atcttggaac agaaaacaat ctcgataacc tgaattctga cattaattct gtagctaaag
     4741 atagtattgg gagaatctgg tttggcacag tcaagggtat ggtagtatta gatccaaagt
     4801 tgctgaaaca aaatactgtt ccggagttga gtaaattaaa catgatacaa ccgatattta
     4861 aaaatcgttc cggaattacg ccagtgttca ggcacaatga aaacacagta acctttgaat
     4921 atgcggcaac ttggtttacc aatcccggaa gaattcttta tcagtataaa ttagatggct
     4981 tgaacgcaga ctggattgca actaaagata ttaaacaagt atttcctaat ttgccaccgg
     5041 gtaaatatgt tttcagaata agaacttcaa tagatccaca gtttacattc aacaatgaaa
     5101 gtagtttttc gtttgagata aaaaaaccat tttggcaaga agattggttt tatttttcaa
     5161 gtctgctgct agcgatttta ttagtttacc tctttataaa agtcagggat gcaagaatga
     5221 aaaagctgca gcaactaaaa aacgattcta tcagatttca gtttgagaca ttgcgcaatc
     5281 aggtaaaccc acattttctg tttaacagtt ttaatacact catagatatc attgagcatg
     5341 ataaagaaca tgcagttgat tatgtagaaa atctttctgc atttttcaga aacattgtca
     5401 cctacaagga taaagagaca ataaaactat cagaagaaat taaaattgca aaggcatttt
     5461 attttattca acaaaaaaga tttggttcaa atctgcaact tttaattgac gaacatagct
     5521 tatatgatga aaaatatgtt gttcctatgg tattacaatt attactcgag aatgcaatta
     5581 aacataatga aatttctaaa gaaaaaaatc tggaggtgca tgttgcattt actaacgata
     5641 gaataataat tagcaataac acaaacccaa aagtgagtaa agatgaatca acaggaaccg
     5701 gacttcgaaa tattattgca cgctatcgtt tactcactga taaaacggta cagattgaat
     5761 catcacaaga ttattttgtt gtatcattgc cattaataaa tcatcagtaa tgaagatagt
     5821 aatttttgaa gacgaagaac cggcatcacg cagactgatt aaactcctga acgagattaa
     5881 tgctaatgtg gagattgttt cagtcattga ttctgtacgt tccggaaaag aatttatgag
     5941 tcagaataaa aatttcgatc ttattatttc agacataaga cttgcagatg gattaagttt
     6001 tgaaattttt aagagctatc ctgtaaagct tccggtaata ttcagtacgg cttatgatga
     6061 atatgcaata gaagcctttc gtaacaacgg aattgattat ttattgaagc cggtaaaaag
     6121 agaagaacta aaatctgcaa tacaaaaata tgagaactgg ttttctgact caacatctgg
     6181 agttaattat gatgctttgt tgcagacact gttgcaagaa aaaaaaggct ttcagaagcg
     6241 aattgtaatt cgttttgcag acactattaa gacagtagaa attgaagatg ctgcttattt
     6301 ttttactgag aataaaataa ataacctttg tacctttggt ggagaatcat ttattgttga
     6361 tcataactta gatgaattag agctaatgct tgacccctct gtctttttta gaatcaacag
     6421 gcagtttatc gttaatttta aagcaataca aaaaatgaat gttgtttcta aatcaagagt
     6481 taagcttaca cttcacccaa caaccattca ggatacaata gttagtacag agcgtagtgg
     6541 cgtttttaaa cagtggttag caggtaagta aaagttcaaa tcaggaactt aaaccttgta
     6601 gttcgtaaag ctttttgtaa acaccttcaa catttgcaag ctgaacatga gtaccgcgtt
     6661 gaacaatttc tcctttctgt aaaacaataa tttcatcggc atgttgaatg gtacttagtc
     6721 tgtgtgcaat gactaataca gtccggtttt tcatcagact tgtcagtgcg tcctgaacta
     6781 atttttcgct ttcagtatct aaagcagatg tagcttcgtc aagaatcaat atcggtggat
     6841 tttttagaac tgcccttgca atgctaatac gttgacgctg ccctccggag agtttactac
     6901 ctctgtcgcc aatgttggtt tgatagcctt ccggcatttg agtaataaac tcatgtgcgt
     6961 tggcaacact tgcagcctct tcaaccattt tttctgttgc atggttcatt ccgaaagcaa
     7021 tgttgttgta aatggtgtca ttaaaaagaa ttggttcctg tgagacaaca cccagcaact
     7081 ctctgaggct atgcaattgt aatttcctaa tatctgtgcc gtcaatcagc agtgtaccgc
     7141 catcaacatc ataatagcga ggcaataaat ctgctaatgt agttttgcct gatcctgatt
     7201 ggcctaccaa tgcaacagtt tttccttttt caatagtgaa acttacattt tttaaaacat
     7261 aaccttcatc accttttctg taagaaaaag aaatattttg atattcaatt ttatagttga
     7321 atgatttaat tgtttctgcc gactgttgat ccttaatact ttcttctgct tccaaaacag
     7381 caaatattct gtcggcagag gcaagcccct tttgaatatt gtaaaatgcc tgagtgaaag
     7441 attttgcagg tggtataatc tgcgaaaata gtgctatata agtgataaat gccgaagccg
     7501 aaagtgatgc atcttttccc aacacaagca taccaccaaa atacattaca atcatcatgg
     7561 tgacagtgcc taaaaattca ctcagaggtg atgataaatc agttttgcga agtgcacgta
     7621 tggcaatggt attataacgc tcattaactt ttgcaaactt ttctgccata aatttttctg
     7681 cattaaatgc tttaattata cgcaggcctg atagtgtttc ttctataatt gaaatcagca
     7741 gcccaagttt ttctttgctt ctggctgatg tacgtttaag acttttgcca atacgtccaa
     7801 tcagaaatcc tactatagga agcagaataa aaacaaaaac agtgagcttg gcacttataa
     7861 aaatcatcat gcctaaaaac aaaaatatat tgaaagggtc gcgaaaaaca acttccaaag
     7921 agctcatcac actccattcc acttcatgca catcagaagt cacccttgta ataatatcgc
     7981 ctttgcgttc attgctaaac catgataaag gtaaccgcat cattttttta tgtacgtcag
     8041 cacgtaaatc tttaactaca ccatttctga tgggagaaat aaaaaattgc cctaagtagc
     8101 gaaaaaggtt tttaagaaaa aacatcactg ccactgcaat acaaataaac attaatgcat
     8161 attgcttacc cctgtttggg tcagtgatca tttcagtaag ataataatag aaactatcaa
     8221 caatagcctt tgttgacaaa tgaaactccg gcttgccggc agccattttc tctaaataaa
     8281 agttatcatc tttcagaaac aataaatcaa gaaaaggagc aattaatgtt aatgaaaaaa
     8341 atgaaaacag aactgataag atattgcacg atgcattcaa taatgcatag ccccaataag
     8401 gttttacgta acgaagtatt ttaaagtaat ttttcaaaaa aaatagttct tgtaaataaa
     8461 atcctgaaaa cagttgcaaa ctaacacaaa aatgttcaat attgtttaat tgttacccac
     8521 agacgctaaa tgcaacatga tccgtatgta attttaggta tatcaccttc ggccactccc
     8581 gaagagatta agcgggctta tcgtaaaaaa gctttcgaac tacatccgga ccgtaacagc
     8641 gaccaacatg ctgaacaact attcagggag ttgaatgagg cttatgcctt actgactgat
     8701 gctgataaaa gaagaaatta tgataagcaa tatcaaaact attcacgcag caataccgat
     8761 cagtttgacc attatttcga aatgcatgtc agcagtaata atgtaaaagt gtgcgaagag
     8821 tttgagttga tttttaccta taccggtgaa ggacgattta tgcaaaaacc agatttgcgt
     8881 gatttttata taaccggtaa accatttgta tcatttcgcg atgtatatcg ttcgggtaaa
     8941 actgcaaggg aaacaaccat tcgctatgtg cttgcagcga aaagagtagg tagttttaca
     9001 ataaatcgtg ctgctattaa gttatttggt aaaaagttta ttactgcaac tcagtatatt
     9061 caggttattt ccggtgaatg ttttttttct cctgataagc ctgctgatgg aagacctttg
     9121 caggtttcac tatggtataa tgccgagaaa ggaggaacaa acagaaaata tttagtcaac
     9181 accaaacaca ccatttatat tcccagaagt aattatgcac aggtttacca tactattggc
     9241 agagtgctta aattaatttt tgcgttttgg ggattttttg ttgcagttaa gattagcaga
     9301 agtggtatta taggattcgc cattggctct gcttatggag caattatgac ctatctgctt
     9361 tactacttcg taaaggtgaa gcccgttttt atgtcagaaa gaaaatattc attggtaatc
     9421 tcctatcttc aaaatggcta ccagccttat cctcctcatg cgcgtaactt gattagccgt
     9481 gcattgatgt ttatcaacaa actgtttttg taaagtgaga taaaaaattt tgaacggaga
     9541 tttagaatga caaattcagt tctattggac aatgatcact gcccattaca ttcatgtgta
     9601 tgggagcact ctttgcgtat ttgcgcagtt cagttgaaat catataataa tctatgcgcc
     9661 agcctacatt tctgtcgcgg gcacgtgtaa tctgatccca gtaggtgtat tgcccggatt
     9721 cctgattaaa ctccctgaaa atgtcaacat accccatgcc gataatcttg tcaatccact
     9781 cgcgttcaat aggcaaaaat cctgttgtct ttgaattttc tttcggccgt gccaaatcta
     9841 tttcctgatg tgcagtatta tagtctccgc aaatgacaat gtttttattt ttctttctca
     9901 tgttttcagc tctctgaaaa agtgattcgt aaaaatccag tttatacttg acacgttcag
     9961 gaccacgacc accatttggg aaatatgcag tgtataacac aaaatcttca aactccattt
    10021 caatacaacg gccttctata tcaaaagcat catcatctaa tccatacctt actttcaatg
    10081 gctcacgttt ggtgtaaatg gcaacaccgc tgtatccttt tttctgtgca gaaaaaaaat
    10141 gacttttgta gccattgaaa tcttttattt catcatccaa ttgctcaggt tgtgccttgg
    10201 tttcctgaag acataataca tcaggcattt cttttgacaa ccattccgga aatccctttt
    10261 tccagatagc tcttatgccg tttacattcc aacaaataat gcgataattt ttgttactca
    10321 ttttttagtg ataaaggttt aatttgattt taatgcatcc cagccttgtg ctttcaatgc
    10381 cactttacgg tcagatttat cgagcatgta ataaccttct tccttagcag tgatgtgacc
    10441 gataatagat atgtcagctt cgctgttaat tttttcaaaa tctttttggt tgatagtaaa
    10501 taacagttcg tagtcttcac caccgttcat ggcacacact gttgggtcta aatgaaactc
    10561 acgtgccaga ttataagtct tttcatcaat aggaatttta gcttcataga ttgtgcaacc
    10621 tttgtctgac tgcgtgcaga tgtgcataat ttcagaagct aatccatcgc tgatgtcaat
    10681 catggctgta ggttttacac ctgttttttt caatatttca ataatttctt tccgtgcctc
    10741 gggctttaac tgtcgctcaa gcaagtagtc gttaccctga aaatcgggct gcactccggg
    10801 actgtctaaa aaaactttct tttctctgtt gagtagttgc aagcctaggt atgcagcacc
    10861 caaatcacca ctcacacaaa tcaaatcatt ttcctgagca ccatttctgt aaaccacatc
    10921 agattttttt gctgtaccaa tggcagtgat tgaaatcact aaaccactgc ggctgctggt
    10981 agtatcgcca ccaatcaaat caacaccata atgtttgcat gcaagcaaca ttccgctgta
    11041 aagttcttca atggcttcga gcgaaaagcg gttactcaaa cctattccca caacaatttg
    11101 tttgggtatt ccattcatgg cgtaaatgtc ggacaggttt accaccaccg atttataacc
    11161 caaatgtttt aacggacagt aaacaaggtc aaagtgtaca ccttcgagca gcatatcagt
    11221 agtaataagc tgaacatttt cttcgctatc taaacaaata acagcagcat catcaccaat
    11281 gccttttagt gtctctttat tcttgatttc aacagcattt tctagatgcc tgataagtcc
    11341 gaactcacct aaactactca actctgtgcg gttgtttgcg ttttcaaaca tgatttatta
    11401 ttctttattg aagatgacaa aagtaaaaat ctggtaatga atagcctttg ataaactttt
    11461 atacctcgta attgttgact ataacagcaa ttaaacctat ctttgcacca cttttataag
    11521 tacttgaaaa tgataacagt aactgaaaaa gccaaatcaa aagccttaag tttaatgcag
    11581 gcagaaaaca aacctgccga tgcatttatt cgcgttggtg tagaaggagg cggatgttct
    11641 ggattaacct atatgttggt ttttgataca gaactaaaac cggaagataa agtgtttgaa
    11701 gataaaggga taaaaatagt gtgcgacaga aaatcgtttt tgtatttagt aggtacagaa
    11761 cttgattata cagatggttt aaatggcaaa gggtttcagt ttaataaccc aaatgcatca
    11821 agaacctgtg gctgtggaga aagtttcgcg gtctaaaatg agtagataat tttttttgac
    11881 taataatttt ttttagaaaa agtctaaata accaaattta aactaaagtg agcgaagatt
    11941 taaaaataat agatgaagta acgagtggcg agtataagtg gggatttgta agtaacattg
    12001 aatcagagaa tgcccccaaa ggattgtctg aagatattgt aagatttatt tcagccaaga
    12061 aaaaagaacc tgagtggatg cttgaattcc gactgaaagc attccgtcac tggcaaaaaa
    12121 tgcaggaacc acagtgggct aatgtcaact atccgaaagt tgattttcag gatatcattt
    12181 attactcggc acccaagccc aagaagcaac tcaacagctt agacgagctt gatcctgaaa
    12241 tcaaagctac gtttgataaa cttggaattt cattactaga acaaaaacgc cttgcaggtg
    12301 tagctgttga tgcagtgata gacagtgtta gcgtaaaaac tactttccgt caggagctaa
    12361 gtaagctggg aataatattt tcatcattca gtgaagctgt tcaggaacat ccggaactgg
    12421 taaaaaaata tatgggcagt gtagtaccct ataccgacaa tttttatgct gcattaaaca
    12481 gtgccgtttt cagtgacggc tctttttgtt atatacccaa aggtgtacgt tgcccaatgg
    12541 agttgagtac ctatttccgt ataaactctg caggcacagg tcagtttgaa agaacactaa
    12601 tcattgccga tgaaggtgct tatgttagct atcttgaagg ttgcacagca ccaaagcgtg
    12661 acgagaatca attgcatgct gcagtagtag aaatttatgc acataccgat gcacaggtaa
    12721 agtatagcac tgtacaaaac tggtatcccg gtgacaaaga tggtcgtggg ggtattttta
    12781 attttgttac caagcgtgga ctttgtgcag gcgaacgttc taaaatcagt tggacacagg
    12841 ttgaaacggg tagtgctgtt acatggaaat atccaagtgt gatattaaaa ggtgactatt
    12901 caactggtga attctattct gttgcattaa ccaacaacta ccagcaggcc gacaccggta
    12961 caaagatgat tcatatcggt aaacatacac gcagcaccat catcagtaaa ggaataagcg
    13021 gaggccgcag taacaatagt tatcgtggat tggtacgtat tggtaaacag gctgagaaag
    13081 caagaaattt ttcacaatgc gattctttgt tgttgggtga taaatgtggt gcacatacat
    13141 ttccatattt ggagtgtgca aacccgacag ctaaagttga gcatgaggct acaacatcaa
    13201 aggttggaga agatcagata ttttattgca atcaacgagg tatctctaca gaagatgcaa
    13261 ttggacttat agtaaatggt tattgcaaag aagtgtttaa tcagttgccc atggagtttg
    13321 ctgtagaagc acaaaaacta ttagcggttt cattagaagg atcagtagga taaaaattaa
    13381 atatagttag ataagaagaa taaagttttt tagaaagtat gttaaacatt aaaaatctac
    13441 atgcctcagt cgaaggtaga gaaatattaa acggattaaa tcttcaggta aatccgggag
    13501 aagtgcatgc cattatggga cctaatggtt caggaaaaag tacgcttgca tcggtacttg
    13561 caggacgcga agcgtatgaa gttaatgatg gtgaaatatt gtatcatggt gagaacctaa
    13621 tagaactttc tcctgaagaa agagcccgta aaggaatatt tctggcattt caatatccga
    13681 ttgaaattcc cggtgtgagt aatgccaact ttctgaaaac agcagttaac gaagtgcgta
    13741 agtcgaaagg tatggatgta ctcgattcaa aagacttttt aaagttgatg aaagataaaa
    13801 tgcagctcgt gaagatggat aaaagcatga cacaacgcag tgttaatgaa ggattcagcg
    13861 gaggagagaa gaaacgcaat gaaatttttc agatggccat gcttgagcct gagttatgtg
    13921 tactcgatga aaccgattca ggtctagata tagatgcact taggattgta tccgatggta
    13981 tcaatgcatt gcgtgacggc aaacgttctt tcattgttat tacacactat cagcgattgc
    14041 tcgattacat agttcccgat tttgtacacg ttttgtataa aggaaaaatt gttaagagcg
    14101 gaccaaaaga acttgctctt gagctggaag aaaaaggata tgattggata aaagaagaag
    14161 ttgaaattta aacctgatta ttgtgaactc atctgttgat acactttctg tgactaacga
    14221 aacaagcatt gccgaagata atatccctgt tgacggattg caaaaattgc gactcgcagc
    14281 attaaataaa tttttagaga aaggatttcc tgattcaaag catgaagagt ggaaatatct
    14341 taatttgaaa cctataagtg aaactaaata taatcaccgt gtagtgtcat cgccactgaa
    14401 agcttcagac ttggagcaat tttacgatgc aggcaacgat actgtgaatt tggtttttat
    14461 aaacggacgt tattgttctg agttttcagg acatcaaagt cacaaacatc ctttcagtat
    14521 ttattcgctc gaaagcagag aagcagaact gtatttagag actttgcgta attcagaaga
    14581 cgaacctttt catgcattaa atacagcctt tcatcaaggt ggtattatga ttcatgttcc
    14641 tgatgattgc cgtatgccaa tgacaataca tctcctatat gttaacgact cgcagaatga
    14701 agccgttgca gcacatccaa aatgtattgt tgttgcagga aaaaattcag catttcaggt
    14761 aattgcaact tatcattcac tcaataattt tccatcactc accaatacag taacagagtt
    14821 ccttattggt gaaaatgcag ttgttgaatt cgatattaag cagaatgaaa acagtaatgc
    14881 caaccatatt agcagagttt atgcccgtgt acaacgtaaa gctgtgttcg atatttttac
    14941 tgttacgatg ggtggtgcac tagttcgtaa caacctcgaa attattttaa gtgaaccaaa
    15001 tgcctgtgct catcttaacg gactcactgt tgctgatggc tatcaggtgg tagataatca
    15061 cacagtggta gatcatgcaa gtccaaactg cgagagcaat caattgtaca aaaatatctt
    15121 agacgatcat tcgcagggag tttttaacgg aaagatattt gttcggaaag atgcacagaa
    15181 gaccaatgct tttcagagca gtaagaatgt gttgttgagt aacactgctg tgatgaatgc
    15241 aaagccacaa cttgaaattt ttgctgatga tgtaaaatgt tcacatggag caaccacagg
    15301 tcagttggac gaagatgcat tgttttattt ccgttcgcgt ggtattggcg aacgtgatgc
    15361 tcgtgctttg ctgaattatg catttgccag tgatgttatt gagaaaatca ataacgatgc
    15421 acttaaatca aacttgttaa aactgttatc gcaaaaacta aatacagcgg ttgattttga
    15481 ggagtagttt aagtttatta gtgtaattta aacgggacta ttggcatcaa ataagaactt
    15541 acatttagtg tagaaatgtt tgtataattt ccatttgatt gttatgaagt gaatctatta
    15601 cgattcaggc agaaaattta agaaaactta taagtttagc ttaacatttt gtttaaattt
    15661 gaccaagata aacttgtaag ttagaaactt gaagacgaat cataaaataa ttagcggaga
    15721 tagcagacac atgtctgagt tgcctgataa ttctgttcat ttggcaataa catcaccacc
    15781 ttattggcag ttaaaagact atggtacaga aaatcagata ggctttcatg atagttatga
    15841 aaattatatc aataacctga atttagtatg gaaagaatgt tatcgtgttt tgcatgacgg
    15901 ttgtaggtta tgtgtgaaca ttggtgacca atttgcaaga gctgtttatt atggaagata
    15961 taaagtcata ccaattcggg aggagattat taagttctgt gaaaatatag gatttgatta
    16021 catgggtgca attatctggc agaaagtcac tacttcgaac acaacaggtg gtggtgttca
    16081 aatgggttct tatccttatc cgagaaatgg aattttaaaa ctagattacg agtttatact
    16141 tgtttttaaa aaattgggcg atagcccaaa acctactaag gagcaaaaag aactttctaa
    16201 aatgtcagct gaagaatgga ataccttttt tgccgggcat tggaattttg caggagcgcg
    16261 acaaaacaat catattgcaa tgtaccccga agaattacca agaagattaa ttaaaatgtt
    16321 ttcgtttgtt ggcgaaacca ttcttgaccc ttttgtagga agtggaacaa ctgctttggc
    16381 agcaaagaat actgaaagaa attctgttgg ttttgaaatt aatcctgcat ttattcaaat
    16441 cataaaagaa aaattggaag tacatcagtt agatttaaat gctactactt atgagtttgt
    16501 taagcaaaaa aaaatcaact tagattttga aaaagaaatt caaaaactcc catatatttt
    16561 taaagatcct catattcttg acaaaaagat agatgtaaaa aaacttcaat ttggttctaa
    16621 gattgataag gacagtaaaa ctaaacgaga agaaatgttt acagttaaag aagttcttag
    16681 tcctgaaaaa attcgattaa acaataacat gactgtaaag cttattggag taaaggaaga
    16741 cccaaacatt aatggtaaag caacttcttt cttacaagag aaaataaaag ggaaacgtgt
    16801 gtttttgaaa tatgataatg taaagcacga tagtgaaaat aacttactgt gttatctata
    16861 tcttgaaaac aaaactttta tcaatgcgca tttaattaaa agtggttttg tgaaggtgga
    16921 tatcggggta gaatttaaat acaaagagaa atttcaaagc ttatttaacg aagttcatgc
    16981 ccaagccgat aaataaacgt ttctcgaagg aattcggtaa aaaggaaaag gttcttaatt
    17041 atacaacaca gacctatcaa ttatcaaggc cgaataaagt cggtgctgta atggcactca
    17101 ttcgtgagtg tcagcctaaa actataaaag aatgggagaa atggtacttt gaaaatgcaa
    17161 agactaacga gaaaacaccg gtaaaaatta ctaagaagag tttaagagaa cttggtgaaa
    17221 gactgcatat aaaaatcaag gagattgtta tcccggaatg gacagaagcg tttagtcaat
    17281 tgacattaca agattgtata gattacattt acaaccttac tataaacaga acttatgatg
    17341 gttttattcg tgagaagtcg gtgattgaag ataatcttgc aaagaaattt ccaaaagtta
    17401 aatttgaaga aagcgaccca gaacttgatc atgccggtga tattgactac ttgggctggg
    17461 ttgaaaaaag agcttttggt attcagataa aacctgttac ttcaaaagct aacttcggca
    17521 attattctcc gtcagaaaga atgaaagcaa gttttagtag ctttacaaaa aagtatggtg
    17581 gaaaagtttt tattgtttat agcatagatg acaagattaa aaatacagaa gtgataaaac
    17641 agattacagc ggaaataaag aaattagcaa agtgagtgtt tttacttaaa tgattaaagc
    17701 agtaatttac ttccttttaa aaggcggaat aatcaatctg atattcctgt cgtagttaat
    17761 atagttccaa agccaattta taaaaacaac tactttattt ctgaacccaa caagcaacat
    17821 taagtgtaaa gccatccaca ccaaccatcc tgcaaatcca ccaaatcgaa tattgaaaga
    17881 ttctgttact gcacgatttc ttccaacagt tgccatagta cctttgtcaa aatatttgaa
    17941 ttttttcagt gacttttgtc ttagttccaa ctcaatgttt tcagccaaca gttgtgccat
    18001 ctgaattgca acaggtgcta cttgcgggtg tccgttaaca tagtctgctg tgctcatgga
    18061 tgcaacatca ccaattgcat aaatattttc acatcctttt actttacaat attcgtcaac
    18121 aacatatcgt ccacgattat tgatggcatc tttatttaat cccgaaatag taacaccttt
    18181 aattcctgca gcccaaagca aatttttact tggcagcact ttactatttt taaaaactac
    18241 ttcttcacca ttataagacg atacctgcgt ttctaaccaa acttcacagc ctaactcatc
    18301 taaatatttt aatgcacgat gttggttttc tttactcatt gcctgtagca cagagtttgt
    18361 accttcaata agcaacacac gcatttttct gaaatcaagt tcgggataat cgttcggcag
    18421 aacatgtttt ttcagttcac ataatgctcc tgcaagctca acacctgtgg ggccaccacc
    18481 aacaacagta aatgtcataa gcgcctcttg ttcttttaaa ctttgtgtga gtaatgcctg
    18541 ttcaaaattt tggagtatta aactgcggag gttgatagct tcaatgacag atttcattgg
    18601 catggcatgt tgcgcaatgt cagcattacc aaaataattt gttgtagtac cggtagcaat
    18661 taccagataa tcataagcta attctccaac tgtggtttga ataatatttt tttcgggaat
    18721 aatattttca acttttcccc agcggaaaag aaaattcttt tgtttcttaa aaattttccg
    18781 gatgggataa gcaatagaat caggctccaa gcccgaagtg gcaacctgat aaagtaaagg
    18841 ttgaaaagtg tgatagttgt ttgtatcaaa catcactatt tgcacacggg ctttgcgcaa
    18901 cgattttgct aattgtaatc ccccaaagcc acagcctaca ataaccacac gaggaatttt
    18961 cagatcaggg atattcatag aaaagttatt ttaaaaacag atgttagttg tacatcatgc
    19021 tatgatttag tgccattggt ctgaataccg ggaaactatt ttttaattac ccaaaactca
    19081 tcactgtaat tgtcatgaaa tgcttcattg tgcactgcag ccatgtcctg caattgtttt
    19141 gctgatgctg cattttgaat ttcattaaac aactctcttt cttcaaaacg aatgtgcctg
    19201 tcaagttcat tttgtaattc atccaattgt tgaatagtct gctccttact ttgaaaaaga
    19261 ttcctaagct tttcatgatg ctgcatagct ttttttatgc ttgcatgttc agcccctaaa
    19321 acaggataca ggtttttttc ttctaactca aaatgatttt cgagatagct tgaccaaaac
    19381 caatcaacat attttttaat tctttcggca ggaattttgt tttttattcc ctgtctgatt
    19441 ttccagccca gtaagagggc atgatgatga tcgcgactta ttggtttaag tgcttcattt
    19501 cttttaatcg gctcactcat gatgaaattg ctttttcaag ttctaatgat tttgggaaaa
    19561 gaatattgtt ttctaaatgt atatgtagat gtaaatcttc ttcaaattct ttgagcatgg
    19621 caaacgttac tctgtaggtg ttgcatgcat cttgtggtgg attgtactta ttgcttaacg
    19681 cttcaatttg tctgaaacgc tcaccttcat tattgtgctc atgcatcatc atttgaatcg
    19741 gattatttat tggtccgaac tgtggttttt ccggtgttgt tttttgcatt tgcgatttta
    19801 ccatccttct cactgcagga aataaaatca actcttcctt tttcatgtgc attgctaact
    19861 cgcctgcaga atcattaaaa agctgattga tttcatacaa ctcaggatga tgatcaccat
    19921 gaactttaca gattttatct aagtaaggct ttatctcctg aattttctct tcaacataac
    19981 ggtgatgctt tttctcaata tagtcagcaa gtaaatctaa tggccatgaa ttaaaatctg
    20041 tttgtgctgc agatgcttgt tgcgaagctt gagtcaactc accaattatg tttgtaactg
    20101 acatctcttt ttgtttacac acctcttcaa gtgtacggtt accattacaa caaaaatcta
    20161 taccgttttg tttaaaaact gttgcagttc tatagtcttt agcaactaag tcgccaacaa
    20221 ttgtgtcttt tgtgatttgc ataattttta tttttaaagt ttttctgcaa ctattgcaat
    20281 tgcattcatg tgtgtttgat attgtttgaa gacttttcgc atttcaagaa ttctttttct
    20341 ggcttttgga tgggtcagga tattccatcc gatttttgtt gctcccatca aaccttcatc
    20401 atctataatc cgattcattt caagaagatg catgccgtta gtcattactt tctttactgc
    20461 aaaaccttct tgctccaaaa gatgtttcca ctcattttca gtaagtgggc gtgcattaac
    20521 ctttattgat aaagctaaat ctctctgaat tttacttttt aaggtgtcat ctacatcagt
    20581 tagtcctaac tcatgaatgg cataccaacc tccttttttg agaatacgat gggcttctct
    20641 gataatttca gattttctat gatcggcatg cattgttagc atggcttcgc catagacttt
    20701 atcctttgag tcgctttcga gatttgtttg tgatgcattt ccgagaataa aagatgcttg
    20761 agaattttgt agtcttttac ttaacattct cactatatct tcgtcagcat caacacctac
    20821 gtatgaatgt agcttgtgcg caagtgtcag cgatgctgta aagcccattc cgggtgcaaa
    20881 ctcaactaca ttatccgaat cattaaaatg taatgcatca attaatttct ttgtaagctc
    20941 tttgcctccg gggcgtagca cttttttgcc cattttggcc aatatccaat ggccatgtgc
    21001 tttctgtatg tctttagtat ccatgattat tttattatgt tgatatttct ttttaatatt
    21061 ctataaataa taatcacaag ttttgaatct atttgacagt gcaggtgttc agtgagaggt
    21121 tatctgaaag aagtaaggtt ttttcagata ttaaagcaaa caaattgctt tcagaatcaa
    21181 aacggtgata ttattatttg ttatttgttg ttttttgaaa ttcgttgctt ccccagttgg
    21241 ctatttcttc tttcgtccat aatgatggga agaattttct ttgctgatat tttggatgta
    21301 aatatttttt ccattcactt ccacctgttg cagctttatt ttctcctttt aaatcaagat
    21361 aatgttgtgc tgtttcaagg tgaactatag gccatgcaac attatacatg tgatcaaact
    21421 cctttaactt cgtttttagt gcatcagagc aatttcccat ttgcatcact ttatcttcta
    21481 ttgtattgcc ctttactttt tttgcaagtg caataaaact ttccaaatat ttttcttcaa
    21541 acagttttaa
//
LOCUS       DAOOOI010000037        21483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_470_length_21483_cov_20.889630, whole genome shotgun sequence.
ACCESSION   DAOOOI010000037 DAOOOI010000000
VERSION     DAOOOI010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 21483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21483
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_470_length_21483_cov_20.889630"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="PKX59_06235"
     CDS             <1..1198
                     /locus_tag="PKX59_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ90502.1"
                     /translation="KNTTGEAENPVDAHAHESATTTTLTSEQIKSIGVTTGGFEKKQL
                     TASLKANGILKVPNQNRANATSLLGGTVKEILVQTGASVQKGQTIAIISNNQFISMQE
                     EYLSISARAGLAEADFERQKTLKEGNAASLKAFQQAEAELKSLRAKKSGLQKQLSLIG
                     IDAQQLTPENIQSSVNIKSPINGSVSNVLVNIGSYLEASNPVAEIVDNSQLHLDLYVY
                     EKDLNKVKVGQTIHFTLTNNPGKEYDADIYAISNTFEPNTKAVSVHATVKGDKHGLID
                     GMSITALISLENATYLAVPTEAIVSNEGQDFIFIVSDKEVPPQEAAAHDHSAHDHAAH
                     DHSAEPVTESGTVFERIPIRKGTTDVGYSEITLLKDIPADSKVVTKGAFFILAKMTNQ
                     GEAHEH"
     gene            1280..1759
                     /locus_tag="PKX59_06240"
     CDS             1280..1759
                     /locus_tag="PKX59_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90503.1"
                     /translation="MRNIKSNIKNIFIATIIGFMLHGCSSKPSETVNSTKVSKAVNDE
                     PVNEPAEPIELDNGQKWKVNEEMKPFLTNGKNLLNEYIQNEDNDYTGLAAALSEQDNK
                     LIESCTMDGKSHEELHKWLHPHLDMVADLESTKDTAQAKKLVEQLKASYVDFEKYFQ"
     gene            1915..4071
                     /locus_tag="PKX59_06245"
     CDS             1915..4071
                     /locus_tag="PKX59_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOZ90504.1"
                     /translation="MTEFFNQLLHEIERPLTNPVLIFSLILFIILLSPILLRRLNIPG
                     IIGLIIAGVIIGPHGLYILEKNSAINLFSTIGLLYIMFIAGLELDLNEFKSHRNKSLV
                     FGFFTFIIPLGIGFPVCHYLLYYDFNASLLTASMFATHTLVAYPIVSKFGVSKNQAVA
                     ITVGGTILTDTAVLIILAVIMGNSQGNLNQEFWIRLGASLIIFSAIMFIVIPRIAKWF
                     FRKLESEKHSHYIFVLSVVFFAAFLAEVAGVEPIIGAFVAGLALNKLIPHSSALMNRI
                     EFIGNSLFIPFFLISVGMLVDVSVIMNGPTALIIAGTLTIVAITGKWLASFLTQVIFK
                     YTSSQRQLIFGLSSAHAAATLAVILVGYKAGILDENILNGTIILILITCIIASFATER
                     AAKKIIVETEDDSSDLLKSSSASNEHILLPVANVGKMEKLLDFAILIKDKKSVNPVSV
                     LSVVSDNSEAEKNILKAKNKLEDFVKQASGTETKVNVITTIDHNAASGITRCSREIMA
                     DIIVLGWPQRTGFLDKLIGEKIESILNNTDKTTLICFLEKPLELHKRIVVAVPPLAEH
                     ENGFGLWLTKLAKLAQELSIPIHTYCNEATEKSIRKFFSKEKLAASLSVSLFSDWDDF
                     LVLARNIHADDFVVLVSARRGATSYMSILDGLPLKIEKHFSDNSRVIIYPQQYDGNIN
                     LENYSEVSTETLNRGIEVVQKIGKEIGSMLKKKNQE"
     gene            complement(4088..4594)
                     /locus_tag="PKX59_06250"
     CDS             complement(4088..4594)
                     /locus_tag="PKX59_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HOZ90505.1"
                     /translation="MEQEIKNRVADSGILQIDPKDFIPATTITELDIKGWLFEEMILK
                     EKDFREHIRQHNWQQYDGVAVAVYCSVDAIIPTWAFMLVSEALQPHTTNIFFGNATQL
                     KEKLTIDTIEKINIQNYNNKRIVIKGCGDENFSPAVYMLLTQKLKPVVKSIMYGEPCS
                     TVPVYKSK"
     gene            complement(4597..4911)
                     /locus_tag="PKX59_06255"
     CDS             complement(4597..4911)
                     /locus_tag="PKX59_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006796533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HOZ90506.1"
                     /translation="MEEPKELYARVIETIKTCYDPEIPVDIYELGLIYEVKIIEGNDV
                     HVKMTLTSPACPVAESLPLEVEQKIKSIPDVNDAKVELTFDPPWDKDMMSEEARMELG
                     MW"
     gene            5074..5478
                     /locus_tag="PKX59_06260"
     CDS             5074..5478
                     /locus_tag="PKX59_06260"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOZ90507.1"
                     /translation="MKQILIVLLTIGLSFSSSAQQKTVLEANEKVAIISFEKIEHNFG
                     KIKEGDKAEHVFLFKNTGNTPLIVTDVSVTCGCTAAEKPDAPIMPGKDGKIKIVFNSA
                     GKVGSQNRTITVSTNAKNKITYLKMIGEVSEK"
     gene            complement(5494..6583)
                     /gene="prfB"
                     /locus_tag="PKX59_06265"
     CDS             complement(join(5494..6522,6524..6583))
                     /gene="prfB"
                     /locus_tag="PKX59_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_106623597.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOZ90508.1"
                     /translation="MTQEQLTECKERVDALRGHLDIDVKLLKIKEEEQLTHAPDFWNN
                     PTRAEQILKSIKLKKNWTDTFAAVQQAVDDLGVLFDFSKTGDASEQEVEEQYTSVQKS
                     LEELEFKNMLSGEEDALSAVLTINAGAGGTESCDWASMLMRMYIRWAERMNYRINEID
                     RQDGDVAGIKSVTLEIEGDFAYGYLKSENGVHRLVRISPFDSNAKRHTSFASVFAYPL
                     IDDTIEIEIKDADIEMQTSRSGGAGGQNVNKVETKVQLKHLPTGIVVVCQVERSQSGN
                     RERAMQMLRSQLYELELRKRNEAKAATEAGKKKIEWGSQIRSYVFHPYKMVKDLRTDC
                     ETSNVQAVMDGELHEFMKAFLMEFGERV"
     gene            complement(6628..7002)
                     /locus_tag="PKX59_06270"
     CDS             complement(6628..7002)
                     /locus_tag="PKX59_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90509.1"
                     /translation="MNKGFITYILQLAGITLLYILLYYLMKDFLAARFYPSWWKCGIF
                     FFVLFSVIHYLRLTKSKGNESIRFFMASTAFKLFLMLAIIVGYIFLNQTMAVPFVISF
                     CLAYFLFMIFDVAKAYTLMKTT"
     gene            complement(7189..7608)
                     /locus_tag="PKX59_06275"
     CDS             complement(7189..7608)
                     /locus_tag="PKX59_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOZ90510.1"
                     /translation="MKNKTTTVKPGETASGSINLIAAGTSIEGEIKSNGDLRIDGHIV
                     GSVFSKAKVVVGTTGVVDGDIVCQNADVSGTVKGTSTVTEMLFLKSTAKINGDINTGK
                     LVVEVGASFTGNCNMGPMVKDIKNVERSEAKLQEKSA"
     gene            complement(7686..10361)
                     /locus_tag="PKX59_06280"
     CDS             complement(7686..10361)
                     /locus_tag="PKX59_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90511.1"
                     /translation="MKSSNHVKYFIILTLFLSACSTQKNTVINRAYHNLTAHYNGYFN
                     ARERVKEGTKTLSALHVDHYDRVLDVFKYATDDKAKTIYPDMDEAIKKSSIVIQRHSM
                     FINGKERCRWIPDNYMIIGKAQFLKHDFWTALETFQYVASTYKDNPKRFDGLIWQMQT
                     QMQLGKMADAEYMLDYLKNEKQLPSKYKPLFAAVAADFYLKKENYPKAIEQLTKAADL
                     ATKRADKIRYSYILAQIYQKVDSNDKAFAIYRKVIKMNPEYEMAFNAKINRARSFDVN
                     SADAQQVKDELNKMLKDEKNKDYLDQIYYALSGISMREKDTTEAFQFLKKSVAASTNN
                     ANQKGISYLEMGEISFRKLDFRAAQLYYDSAATNITSDYPDYALIQNKKTNLSRLIKN
                     LNIISHEDSLLTLGALSETEQYSRIDDIIAAEEAESLRKKQEEEAAKAKEQEEQSNMQ
                     FSQFQNQPGKDGMPGNSTGSSWYFYNQSAISFGFSEFAKKWGNRKLEDNWRRSSKESI
                     ATFGSEEEAEAQARADSLEQAQKALNDSIRQLNSEARRKAYFNNIPKTAQQKEESNAK
                     ILEAYYNAGIIYKEALGQPKPAADDMEEMMKRYPGNKYELPAYYNLYRCYLALFDEPK
                     AEYYKKIILNDYGDSEYARIITNPNYFKDAQRKTAIMQVFYENTYKAFLNRQYTDVIE
                     RKSAADSLFPGGTLAPKFAYLQALSIGYTQPRPQFEASLKKIVAAYPRDSVSILAKAI
                     LDKMNNVTPIDTTISESATTQTAEGNKMLNSIYEFMPDKPHNVLIIFSAELPYATTLK
                     GKINQFNQRNYKEKNLSVTELRVDDKNYMMVRSFPDHTQSKEYIQGLDSDQTVFEGMD
                     KSAFTLFPITEKNFNKFITDKDLSKYLDFYRVNYP"
     gene            10485..11363
                     /locus_tag="PKX59_06285"
     CDS             10485..11363
                     /locus_tag="PKX59_06285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOZ90512.1"
                     /translation="MAKASGKEKVKLSTKLRNKFRLVIMNDETLEEKASLLLSPINVV
                     VVVGIAIITLITATTYIIAFTPLREYIPGYTDVKVKRAAIESLIRTDSLQKQVSAQDL
                     YIQNLQNLITGQSQKTVSLDTVSVPQLYDTIKQLLHSEEDSLLRSQIESPQDYNLTLN
                     AGTTKGSIANFYFFAPLKGAITTRFNPTEKHFGVDVVAGPDAVIKSAMDGTVVIAEWT
                     SQAGYVIAIQHSNNMFSVYKHNSALLKKVGNVVKAGEVIAIVGNSGELSNGPHLHFEL
                     WYNGAPVDPTDYIAFE"
     gene            complement(11364..12416)
                     /gene="mutY"
                     /locus_tag="PKX59_06290"
     CDS             complement(11364..12416)
                     /gene="mutY"
                     /locus_tag="PKX59_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOZ90513.1"
                     /translation="MSISNKLILWYERNKRELPFRSTNDAYRIWLSEVILQQTRVEQG
                     LPYYKRFLESFPTVKHLAKASETEVLKLWQGLGYYSRGRNLLATAQHITKIFHGKFPS
                     NYFELKKLKGVGDYTAAAIASMAFNEPIFAVDGNVARVLSRLFAEKTTPNSTEGKKVF
                     QELADSIGNKKFPGLHNQAMMELGALVCTPRNPKCEICPLQIHCLAYQQKQQLNFPVK
                     SKKIKIKERHFCFVIILYKDKFYIRQRNGNDIWKNLYEFPLFETRTKFSSATISNFAK
                     EYGIKKYESGRNHIHKLTHQHINASYIVAYLSKPLSSKKFPELKAVDAEDFKNNFPHP
                     RLIEKILSENNYFNLL"
     gene            12520..13362
                     /gene="panC"
                     /locus_tag="PKX59_06295"
     CDS             12520..13362
                     /gene="panC"
                     /locus_tag="PKX59_06295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOZ90514.1"
                     /translation="MKLIKEIHSLSHIVSRKKHENISIGFVPTMGALHEGHISLTKQS
                     LLENEFTIASIFVNPLQFNNPDDLKKYPRTLDRDMQMLEAAGCNALFAPEASEVYIED
                     EQPLQFDLGNLANVMEGKFRPGHFAGVITIVHKLFSWVKPDVAYFGEKDFQQLAVIRK
                     MAKALHPDIIVTGCPTLREPNGLAMSSRNVRLSNTVRHDASIIYKAMQHAAQLKNILP
                     VYEVCSQVRSMIEEIAPFKVEYVEIADAVNLQSLQQWSDSQSPRIFVAVFADDVRLID
                     NAAL"
     gene            complement(13367..14242)
                     /locus_tag="PKX59_06300"
     CDS             complement(13367..14242)
                     /locus_tag="PKX59_06300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010603248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ90515.1"
                     /translation="MELLTPQNFKDYELLDSGNFEKLERFGQYITIRPEPQAVWNRQL
                     KEADWLKKAHVKFIAKNSSSGTWKKLKEIPDRWHIAYALPDEKQIRLRLALTSFKHVG
                     VFPEQAVNWDFIYNSVKKLDVVQPKVLNLFAYTGASTLAAKAAGADIIHLDSIKQVVT
                     WAKENMTLSGLDNIRWLVEDAMTFVKREVKRGKKYQGIILDPPAYGHGPNGENWKLED
                     EINEMLANVKLLLEENHFLVLNTYSLGFSSIILDNLLDEHFSKNIKRQTGEIFLQSTA
                     GARLPLGVFARHCTV"
     gene            14332..15465
                     /locus_tag="PKX59_06305"
     CDS             14332..15465
                     /locus_tag="PKX59_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ90516.1"
                     /translation="MIINTSELSGRSIQATDKTPLRILLVDDIPANLLTLEGILEDDS
                     RILIKAFSGNEALKLLMEEPIDLILLDIQMPDMDGVEVAQLLRANPKTKHIPIIFVSA
                     IAKSERPSLEEFEIGSFDFIPKPLDIEETQKIVSIFEFGCRYKQKYREQEEKLKRLND
                     HLDKFLYILSHDLNAPLRAIENLVQWLEEDLQSQISDESKENIGLLRNRVTKMKLLLD
                     GLLRYARSGNTKHQHDIISPEKMIRQITSELQGDRKVSLNVNSSVLDMNTDRTALQLV
                     LKELIQNAISFNPSDKNTIDISVVEKNDSYEFVIADNGPGIKPQQQKTIFELFQTLPS
                     SVVPSSGVGLSIVKKVVEEYGGTVWFSPTVKCGTEVHFNWKKS"
     gene            complement(15469..17862)
                     /locus_tag="PKX59_06310"
     CDS             complement(15469..17862)
                     /locus_tag="PKX59_06310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HOZ90517.1"
                     /translation="MSNTDTVNKVKCYHCGDDCDEQVITSDEKIFCCTGCKLVYEILR
                     ENNLCNYYNLNNNPGTKQSDIVQKRFAFLDEPSIENQLISFRDKNETIITFYIPAMHC
                     SSCIWLLENFQKADEGVLSSRVDFVRKELRLVYDHHKTSLRKIAEKLSQTGYEPALQL
                     DAIEKKKIKKANRSRILRIGVAGFCSGNIMMLSFPEYFSSGNNIDASLQTLFSYLILL
                     LALPVFFYSANEFFIKSWQALKQKTTSIDLPIAIGISVIFLRSAFEIITQTGSGFFDS
                     GSGLVFFMLIGRWFQDFTFDSLAFDRDFKSYFPIAVTSIKNGNEKEVQINDLKVNDRI
                     LIRNNEIIPADCILLKGNASVDYHFVTGESLPVSHTAGEYIYAGGKQAGAMIELQVMK
                     EVSQSYLVQLWNRSWDKQDISKFEKLVNAISRWFIVVTFSISTIAAIYWWNTDIHKAI
                     NAFTAVLVIACPCALALSAPFTYGNILRILGRNKIYMRNYRVLEKIADADTIVFDKTG
                     TLTKNNVSEISYHGQSLDDEDKKILSSLFRQSSHPLSKLLQQHLQADKYYPCNNFEEV
                     TGKGICAVLNNKNIKAGTSDWAGVNNEVEKNATAVFININDIKKGKFVFSNIYRDGLN
                     TISQKLNNKHNLIVLTGDNESERKNLTTLLGNAELRFNQKPEDKRNYILELINSKKNV
                     LMIGDGLNDAGALLAANTGISVTDNTNNFTPASDTIMDAAALHQLPELIKLSKSAVTI
                     IIISFIISLLYNFVGLAFAVSGTLSPLFAAILMPISTISLVLFTVGASTIKARQSGF"
     gene            17998..19374
                     /locus_tag="PKX59_06315"
     CDS             17998..19374
                     /locus_tag="PKX59_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ90518.1"
                     /translation="MQIKQFYNQGHSQFSYIISSGKEAVIIDPVRDVEHYLQYLADKQ
                     LILKYIFETHIHADFVSGHLELADKTGATIVYGPQVRVAYPSYSARHNESFNLEGCSI
                     KALHTPGHTMESVCYLLNNNVGQPIAIFTGDTLFVGDVGRPDLMSGNKNAVELSEYLY
                     HSINDIIKTLPDELVVYPGHGAGSFCGKNPGKEKFSTIGEQKKRNYALKDISLEKFTN
                     EITTGLSVPPEYFFKVMSINIMGCRPMKDVFRNNLNFLSPVNFKAETQRALIVDTRNE
                     LEFSKAFIKGSVNIGLDGSFATWAGILLDPDHPVLLVCDSGKEKEAIGALAQIGIENV
                     RGILHGGIKSWKDAGQPVDNVENIDAEDFYFLFENEGYILVDLRSHQNFNAQHIKRSL
                     SIPLDELPHRLNEFSPDTKYLLVCKGGYHSMIAASILKRNGINQVVNISGGFDAVSAF
                     QPQYVESI"
     gene            19657..20478
                     /locus_tag="PKX59_06320"
     CDS             19657..20478
                     /locus_tag="PKX59_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOZ90519.1"
                     /translation="MKKIVVTTDFSECSENALIYSLSLCKEVNASLYLLHVFHIPVPV
                     TDMPYISVSSEEEEKNIELRLKELITRIRHKTGYEGMIHTITDFNFTVDGILNVVNDT
                     NADLLVMGQRGKTTLADRLFGSTATAIINLATCPLLLIPEGCSFSKIKKIAIACDKKT
                     SDNKNKVFHVVEMNAALKSEMLLFNVVQDEAEKDRLKGEDFTEMAGKEVKLFFPVHHD
                     IHEGILQFVNDNDADWLCMFPHRHNIFEQWFTRLNSGNISQQVKIPLLLINELVQ"
     gene            20557..20970
                     /locus_tag="PKX59_06325"
     CDS             20557..20970
                     /locus_tag="PKX59_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90520.1"
                     /translation="MKHRLTIQAEDTILDFQKKFNRIYPFLKIEFFEKAHIAKQGSHK
                     KEMIASNMLFRGLIGRGKSHIINFNDDFSISDFEMLLNDQCRLYAQVFRKSGKIWLET
                     SATDYWTLEQQNEEGKSLAKHFKTESENINDIDPY"
     gene            complement(21164..>21483)
                     /locus_tag="PKX59_06330"
     CDS             complement(21164..>21483)
                     /locus_tag="PKX59_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90521.1"
                     /translation="YFDFNDPVITNTATTHVVLPTGIASYQSAENIQLFPNPAQSELN
                     IQLTTAVEKIQVIDVLGKILFEKKTNNQQNLKINISQFQNGVYFVKAYSTQGVVVKKF
                     VKR"
BASE COUNT         6707 a         3799 c         4013 g         6964 t
ORIGIN      
        1 aaaaaacaca accggtgaag ccgaaaaccc tgttgatgca catgcacatg aaagtgcaac
       61 tacaaccaca ctcacttcag agcaaataaa atctattggt gtcacaacag gagggtttga
      121 aaagaaacaa ttgacagctt cactgaaggc aaacggaatt ttaaaagttc cgaatcaaaa
      181 tcgtgcaaat gcaacttcat tacttggcgg tacagtgaaa gagatattag ttcagacagg
      241 agcatcggta cagaaagggc aaaccattgc tattatcagt aacaatcagt tcatcagtat
      301 gcaggaagaa tatctcagta taagtgcacg tgcaggtctt gcagaagcag attttgaaag
      361 acaaaaaacg ttgaaagaag gtaatgcagc atcgttgaaa gcctttcagc aggcagaggc
      421 agaactgaag tcattacgtg caaaaaaatc aggcttacaa aagcaattgt ctttaatcgg
      481 tattgatgcc cagcagttga ctcctgaaaa tattcaatcg tcagtaaata ttaaaagccc
      541 cattaatgga tctgtaagca atgtactggt aaatatcgga agttatcttg aagcaagtaa
      601 tcctgttgca gagattgttg acaacagtca attgcatctt gacttgtatg tgtatgaaaa
      661 agacctcaac aaggttaaag taggacagac aatacatttc acactcacca ataatccggg
      721 taaagaatac gatgctgata tttatgctat cagtaatacg tttgagccaa acacaaaagc
      781 agtctctgta catgcaactg ttaaaggtga caaacatgga ctgattgatg gcatgagcat
      841 tactgctttg ataagcttag aaaacgcaac ttatttggca gtaccgacag aagcaatcgt
      901 ttcaaatgaa ggccaggatt ttatttttat tgtctctgat aaagaagttc caccacaaga
      961 ggccgctgca catgaccata gtgcacatga tcatgcagca catgaccata gtgctgaacc
     1021 cgttacagag tccggtacag tgtttgaaag aattcctatc agaaaaggaa caacagatgt
     1081 gggttattcg gagattactt tattaaaaga tattcctgcc gatagtaagg tggttactaa
     1141 aggtgcattt tttattctgg ctaagatgac caatcagggt gaggcacatg agcattgatt
     1201 attaaaaact taataaagta acatctgagt aacttatttg gatttgattg attaaatttg
     1261 acaactgaaa gaatatatta tgagaaatat aaaaagcaac atcaaaaata tttttattgc
     1321 aactattatc ggatttatgc tgcacggatg tagtagtaaa ccaagcgaaa cagtaaacag
     1381 cactaaggtg agtaaagcag ttaatgatga gcctgtaaac gaacctgcag agcctattga
     1441 gctggataat ggccaaaagt ggaaagtaaa tgaggagatg aaaccatttc ttacaaatgg
     1501 caaaaacttg ttgaatgaat acattcaaaa cgaagataac gactatacag gtttagcagc
     1561 agcacttagt gagcaggata ataagttgat tgaaagctgt acaatggatg gtaaaagcca
     1621 cgaagaatta cataaatggc tgcatccaca tttagatatg gttgccgatc ttgagagtac
     1681 aaaagataca gcacaagcta aaaagttggt ggaacaactg aaggcctctt atgttgattt
     1741 tgaaaagtac tttcaataag tactgaaata ttttttgttt acttagtaaa ctgcaggcag
     1801 aagtaggttc tactgagtgt gataataatg agaggcaaaa cactgctata tatgacattc
     1861 gttagtcaga ttaaataaac cacttaactc aaaaatgata ggacgatagc ataaatgaca
     1921 gaatttttta atcaactact tcatgagatc gaacgaccac ttaccaatcc ggtactgatt
     1981 ttttctttga tattatttat cattctgctt tctcccattt tgctgaggcg tttgaatatt
     2041 cccggaatca ttgggctcat cattgcagga gtaattatcg ggccacatgg tttatatatt
     2101 ctcgaaaaaa actcagcaat caatctgttt tcaaccattg gtttgcttta catcatgttt
     2161 attgccggac ttgaactcga tctgaatgaa tttaaatctc atcgcaataa aagtttggtc
     2221 tttggttttt ttactttcat cattccttta ggtattggct ttccggtttg tcattacttg
     2281 ttgtattatg actttaatgc aagtcttctg acagcaagca tgtttgctac acatacgctt
     2341 gttgcctatc ccattgtaag caagtttggt gtttcaaaaa atcaggccgt tgccattacg
     2401 gtaggaggaa ctattttaac agacactgcc gtactgatta tccttgcagt aatcatgggc
     2461 aacagccagg gaaacctgaa tcaggaattc tggattcgtt taggggcatc actgattatt
     2521 ttttctgcaa taatgtttat tgttattccg agaattgcaa aatggttttt cagaaaactc
     2581 gaaagtgaaa aacattcgca ttatattttt gtactctctg tggtgttttt tgcagccttt
     2641 cttgcagagg ttgccggagt agaaccaatc ataggagcct ttgttgcagg tttggcattg
     2701 aacaaactga ttccacattc atcagcattg atgaacagaa ttgagtttat tggaaactct
     2761 ttgtttattc catttttcct gatcagtgta gggatgttgg ttgatgttag tgttatcatg
     2821 aatggaccta ctgcactcat catagcaggt acattaacaa ttgttgcaat aacaggaaaa
     2881 tggcttgcat cttttctgac acaagttatt tttaaatata catcatcgca acggcaactt
     2941 attttcggtt taagcagtgc tcatgctgct gcaacacttg ccgttatttt agtaggttat
     3001 aaagcaggca ttcttgatga gaatatcctc aacggcacca ttattcttat tttaattaca
     3061 tgtatcattg cttcttttgc aacagagcgt gccgctaaaa aaataattgt agaaacagaa
     3121 gatgacagca gcgatctttt aaaaagtagc agtgcttcca atgaacatat tttacttcct
     3181 gtagccaatg ttggtaaaat ggaaaagctg cttgactttg caattttaat aaaggataaa
     3241 aaatctgtta acccagtgtc tgttctttct gttgtatctg acaatagcga ggcagagaaa
     3301 aatattttaa aggcaaaaaa taaattagaa gattttgtta aacaagcctc aggaacagaa
     3361 actaaagtta acgtaattac tacaattgat cataatgcag caagcggaat tacccgttgc
     3421 tcaagagaaa ttatggccga catcattgtt ttgggatggc cacagcgtac aggatttctt
     3481 gataaactta ttggtgaaaa aattgaaagt atattaaaca atacagataa gacaacttta
     3541 atttgctttc ttgaaaaacc acttgaactt cataagcgaa tagtggttgc tgtaccacct
     3601 cttgccgaac atgaaaatgg atttggttta tggcttacta aattggcaaa gctggcacag
     3661 gaactcagta ttccaattca tacatattgt aatgaagcaa ctgaaaaatc aataagaaaa
     3721 tttttcagta aagaaaaatt agcagcatca ctgagcgtta gtttgtttag cgactgggat
     3781 gattttctgg tactggcaag aaatattcat gctgatgatt ttgttgtttt ggtttctgcc
     3841 agacgtggtg caacttctta catgagtatt ttagatggac ttccgctgaa aatagaaaaa
     3901 cattttagcg acaacagccg agttattatt tatccgcaac aatatgacgg caatattaat
     3961 ctggaaaact atagcgaggt ttcaacagaa actttaaaca gaggtattga ggtggttcag
     4021 aaaatcggta aagaaattgg ctcaatgcta aaaaagaaaa atcaagagta gatttattct
     4081 tgattaatca ttttgattta taaacaggaa cagtagaaca tggttcgcca tacataatac
     4141 tcttaacaac aggttttagt ttttgtgtca acagcatata aacagcaggc gaaaagtttt
     4201 catcaccaca ccctttaatt actattcgtt tgttattata gttttgaatg ttgatttttt
     4261 caattgtatc aatggttagc ttttctttga gttgagtagc gttaccaaaa aaaatgttgg
     4321 tggtatgtgg ctgcaatgct tcactaacga gcataaatgc ccaagtcgga ataatggcat
     4381 caacagaaca ataaacagca actgcaacgc catcatactg ctgccagtta tgttgtctga
     4441 tatgctctct gaaatctttt tctttaagaa tcatttcctc aaacaaccat cctttaatgt
     4501 caagttcggt tattgttgtg gcaggtataa aatcttttgg atctatctgt agaataccac
     4561 tgtcggcaac gcgattttta atttcctgtt ccataattac cacattccaa gttccatgcg
     4621 agcctcttca ctcatcatat ctttatccca tggtggatca aaagttaatt caacttttgc
     4681 atcattcaca tcaggaatac tttttatttt ttgttcaact tccaatggca gcgattctgc
     4741 aaccggacaa gcaggtgaag tcagagtcat ctttacatga acatcatttc cttcaataat
     4801 ctttacttcg taaatcaatc ctaactcata aatgtcaacc ggaatttccg gatcgtaaca
     4861 agtctttatg gtttctataa ccctggcata taattctttt ggctcttcca taagtgcaaa
     4921 tttaatggtt ttattgttgt cttgatttta ttgctgtttt attctgattt tacaatcaat
     4981 agaagattca aaataaattc atgcaaagca tcaatattaa tcccatcttt gaaaactata
     5041 aagtcaagta aaagtttttt aaaaataaat attatgaaac aaattttaat cgttctcctt
     5101 acaattggtc tatcattttc atcatcagcg cagcaaaaaa ctgttttgga ggccaatgaa
     5161 aaagttgcta taatcagttt cgaaaaaata gaacataatt tcggtaagat taaagaaggt
     5221 gataaggccg aacatgtttt tctttttaaa aatacaggca acacaccgtt aattgttact
     5281 gacgtgagtg ttacctgcgg atgtacggca gcagaaaaac ctgatgcgcc aattatgccc
     5341 ggtaaagacg gaaaaataaa gatagtattt aactctgccg gtaaagttgg ttcacaaaac
     5401 cgcacaataa cggtaagtac caatgcaaaa aataaaatta cttatttgaa aatgataggt
     5461 gaagtgagtg aaaaataatt tctgagaaaa tggttaaact ctttctccaa attccatcag
     5521 aaaggctttc ataaactcat gtagttcgcc atccattacg gcttgaacgt ttgatgtttc
     5581 acaatctgtg cgtaagtctt taaccatctt atatggatga aaaacatagc ttcttatttg
     5641 tgagccccat tcaatttttt tcttacctgc ttctgtcgct gccttagcct cattgcgttt
     5701 gcgaagttca agttcataaa gttgcgaacg caacatctgc atggcacgct cgcggttgcc
     5761 actttgcgaa cgttctacct gacatacaac aacaatacct gtgggcaagt gtttgagttg
     5821 aacctttgtt tctactttat ttacattttg tccacctgca ccacctgaac gggaagtctg
     5881 catttctata tctgcatctt taatttcaat ctctatagta tcgtcaatca aaggataggc
     5941 aaacacactg gcaaatgatg tatgacgttt tgcattggaa tcaaatggag aaatgcgtac
     6001 taaacgatgt acaccatttt cgctctttaa atatccgtat gcaaaatcac cttctatttc
     6061 taaagtaaca gattttattc ctgcaacatc gccatcttgt ctgtcaattt cgtttatgcg
     6121 atagttcatc cgttcggccc agcggatata catacgcatg agcattgatg cccagtcgca
     6181 actttctgtt cctccggcac cggcatttat tgtgagcact gcactcaatg catcttcttc
     6241 accactgagc atgtttttaa attcaagctc ttctaatgat ttttgaacgg aggtatattg
     6301 ctcttctact tcttgttctg aagcatcgcc tgtttttgaa aaatcgaata atacacccaa
     6361 atcatcaaca gcttgctgta cggcagcaaa cgtgtctgtc cagttttttt taagcttgat
     6421 ggattttaaa atctgttctg cccttgtagg gttgttccag aaatcaggtg cgtgtgtaag
     6481 ttgttcttct tcttttattt taagcagttt gacatcaatg tcaaaggtgc cccctcaaag
     6541 cgtccacgcg ctccttacat tctgttaatt gttcttgtgt catacgggct gcgaatttac
     6601 gaattaatgt tttccggcat tttcagacta tgttgtcttc atcagcgtat aggcttttgc
     6661 tacatcaaaa atcataaaca aaaaataggc taagcaaaaa ctaattacaa aaggaactgc
     6721 cattgtttga ttcagaaaaa tgtagcctac aatgatagca agcatcagaa ataatttaaa
     6781 cgctgttgat gccataaaaa aacggatgga ctcattacct ttactcttgg taagtcgcaa
     6841 ataatgaatg acagaaaaaa gtacgaagaa aaatattccg catttccacc atgaaggata
     6901 gaaacgtgca gccagaaaat ctttcatcaa ataataaagc aaaatataca gcagggttat
     6961 gccggcaagt tgtaaaatat aggtgatgaa acctttgttc attttttctt atgtaaaaaa
     7021 tctttcaatg ttaagtaaag tgcagctgca acagcaatca aggcaaaagt aacagtgaat
     7081 agtggaattt tgttttgaca atatttgtct aagcggtaac ctccataggt accaataaac
     7141 atgatagcgc ccatttgaaa tcccatggac gcataccggg cataatcgtt aagctgattt
     7201 ttcctggagt ttggcttctg atctttcgac attttttatg tctttaacca taggccccat
     7261 attacagttg cctgtaaaac ttgccccaac ttcaacaact aatttgccgg tgttaatgtc
     7321 gccattgatt tttgcagttg actttagaaa caacatttca gtaacggtag atgttccttt
     7381 tactgttcct gaaacatctg cattctggca aacaatgtct ccatccacta ctccggttgt
     7441 gccaacaacc actttagctt ttgaaaatac tgagcctaca atgtgtccgt caattcgcaa
     7501 gtcgccattc gacttgattt caccttctat tgaagttccg gcagcaatta agttaattga
     7561 gcctgatgca gtttcaccgg gtttaacggt ggtggttttg ttcttcaaat tttcaaacat
     7621 ggtaaaaact tttaattatg tattgcggca aagataaaaa taaaagttat taacaaaacc
     7681 aaaaattatg gataattaac acggtagaaa tcaaggtatt tgctcaaatc cttatcggta
     7741 atgaacttgt tgaaattttt ttctgtaatg gggaaaaggg taaaagcact tttatccatg
     7801 ccttcaaaaa ccgtttgatc agagtccaac ccctgaatat attccttgct ttgcgtgtgg
     7861 tcgggaaatg aacgcaccat catatagttt ttgtcatcaa ctcttaactc tgtcacactc
     7921 agatttttct ctttatagtt gcgctggtta aactgattga tttttccttt taatgttgtg
     7981 gcataaggca gttcagcaga gaaaataatc agcacattat gtggtttgtc cggcataaac
     8041 tcatagatag aattcagcat tttattgccc tcggcagttt gtgttgttgc cgattcgctg
     8101 attgttgtat ctattggcgt tacgttattc attttatcaa gaatagcttt tgccaatatg
     8161 ctgacagaat ctcttgggta tgcagcaaca atttttttca gtgaagcttc gaactgtggg
     8221 cggggctgtg tatatccgat agaaagtgcc tgaaggtagg caaactttgg cgctaatgtg
     8281 ccaccgggaa atagtgagtc tgctgccgat ttacgctcta tgacatctgt atattgacga
     8341 ttgagaaaag ccttgtatgt attttcataa aacacctgca tgatggctgt cttgcgctgt
     8401 gcatctttaa aataatttgg gttggttatg atgcgtgcat attctgaatc gccatagtca
     8461 ttgagaataa tttttttata atactcggct ttaggttcat caaataaagc taagtaacaa
     8521 cgatacagat tgtaataagc aggtagttca tatttattac ccggatatcg tttcatcatt
     8581 tcttccatat cgtcagcagc aggctttggc tgacctaatg cttctttata aatgattcct
     8641 gcattgtaat atgcttctaa tattttagca ttactttctt ctttttgctg agcagttttt
     8701 ggaatgttgt taaagtatgc ttttctgcgt gcttcgctat tcaattgtct gatgctgtcg
     8761 tttaatgctt tttgtgcctg ctctaaactg tcggcacgtg cttgcgcttc ggcttcttct
     8821 tcactgccaa aagttgcaat ggattctttg ctgctcctac gccagttatc ttcaagtttt
     8881 ctgttgcccc atttcttggc aaactccgaa aagccaaaac taatggcact ctgattataa
     8941 aaataccatg agctacctgt tgaatttcca ggcataccat cttttcctgg ttgattttga
     9001 aactgactga actgcatgtt agactgctct tcctgctctt tagctttggc tgcctcctct
     9061 tcctgtttct tacgcagcga ctctgcttct tctgcagcaa taatgtcgtc aatgcgcgaa
     9121 tattgttctg tttctgaaag tgctcccaat gtgagcagac tgtcctcgtg tgaaatgatg
     9181 ttcaggtttt ttatcaatcg cgacagattg gtttttttat tctgaatcaa tgcataatca
     9241 ggatagtcag aagtgatatt ggtggcagca ctgtcataat ataattgcgc agcacggaag
     9301 tcaagtttgc ggaaacttat ttcacccatc tccagatatg aaataccttt ttgattggcg
     9361 ttgttggtgc ttgctgccac agacttcttt aaaaattgaa atgcctctgt tgtatctttt
     9421 tcacgcatag aaattccact cagtgcataa taaatctgat caagataatc cttgtttttt
     9481 tcatctttaa gcatcttgtt caactcatct tttacttgtt gtgcatcggc actgtttaca
     9541 tcaaatgaac gcgctctgtt gatttttgca ttaaatgcca tctcatactc gggattcatc
     9601 ttgattactt tgcggtagat tgcaaaagct ttgtcgttgg aatcaacctt ctgatatatc
     9661 tgtgcaagga tatagctata tcggattttg tcagctctct ttgtcgccag atcagcagct
     9721 ttggttaatt gctcaatagc tttaggataa ttttctttct tcagataaaa atctgcagcc
     9781 acagcagcaa aaagaggttt gtactttgat ggcaattgct tttcattttt taaatagtcg
     9841 agcatgtatt ctgcatcggc cattttgcct aactgcattt gtgtttgcat ctgccagatt
     9901 aagccatcaa atctttttgg gttatcttta taggtagatg caacgtattg aaaagtctct
     9961 aatgcagtcc agaaatcatg ctttagaaat tgcgctttac cgattatcat gtagttgtcc
    10021 ggtatccagc ggcaacgctc tttaccatta ataaacatgg agtgtcgttg aataactatt
    10081 gagcttttct taatggcctc atccatgtca gggtaaatgg tcttggcttt atcgtcagtt
    10141 gcatatttaa aaacatcaag cacacggtcg taatgatcaa catgcaatgc agacaaagtt
    10201 tttgtacctt ctttcactct ttcgcgggca ttaaaatagc cgttgtagtg tgcagtaagg
    10261 ttatggtatg ccctgtttat gacagtgttc ttttgtgttg aacatgctga tagaaacagc
    10321 gtaaggatga taaaatattt tacgtgattg gatgatttca aaacaatatg gttgttgcga
    10381 taaatattaa ctttttccag cgaaaattat tgctcgttta attattacga tatttgcccg
    10441 attaagaagg caaacataac aatacttttt tgtttaaaca acgcatggcc aaggcatcag
    10501 gcaaagaaaa agtcaaacta agcaccaaac tccgtaataa gttccggtta gtcattatga
    10561 atgacgaaac tctggaagaa aaggcttcgt tgctgctctc accaatcaat gtggtggtgg
    10621 tggtgggtat tgccatcatc accctcatca cagccacaac ctacattatt gcctttacac
    10681 cactgcgtga atatattccc ggatatacag acgttaaagt aaaacgggca gccattgaaa
    10741 gccttatccg taccgattct ttacaaaaac aggtgtctgc acaggatttg tacatacaaa
    10801 atctgcaaaa tcttattacc ggtcagtcac agaaaactgt ttcgctcgat acagtttctg
    10861 tgccacagct ttatgataca ataaaacaat tgcttcattc cgaagaggat tctttgctga
    10921 gaagtcagat agagtcgcca caagattaca acctaacatt aaacgcaggg accactaaag
    10981 gttctattgc taacttttac ttttttgcgc cattaaaagg agcaataaca acacggttta
    11041 atcctacaga aaagcacttt ggtgttgatg tagttgccgg ccctgatgct gttattaaaa
    11101 gtgccatgga cggaacggtg gtcattgcag aatggacttc gcaggcaggt tatgtcatag
    11161 ccattcaaca cagtaacaat atgttttctg tttataaaca taattctgca ttgcttaaaa
    11221 aagtaggtaa tgtggtgaaa gccggagaag ttattgctat tgtaggtaac tcgggcgaat
    11281 taagtaatgg tccgcatctg cattttgaac tttggtacaa tggtgcgcct gttgacccta
    11341 ctgattatat agcctttgaa taattacagc agattaaagt agttgttttc agaaagtatt
    11401 ttttcgatta accttggatg tgggaaattg tttttgaaat cttcggcatc aactgctttt
    11461 aactcaggaa atttttttga tgatagtggc ttgcttaaat atgccacaat gtatgatgca
    11521 ttaatgtgtt gatgtgtcag cttatgaata tgatttcttc ctgattcata ttttttaata
    11581 ccatattctt ttgcaaaatt gctaatagtg gctgatgaaa attttgttct ggtttcgaac
    11641 aaaggaaact catagaggtt tttccagatg tcgttaccat tacgttgtct gatataaaat
    11701 ttgtctttgt aaagtattat gacaaaacaa aagtgacgct ctttgatttt aatcttttta
    11761 ctttttaccg gaaaattaag ttgttgcttt tgttgatatg ccaaacaatg aatttgtaaa
    11821 ggacatattt cacacttagg atttcgtggt gtacaaacca atgcaccaag ctccatcatt
    11881 gcctgattgt gcaggccggg aaatttttta ttgcctatac tatcggctaa ttcctgaaac
    11941 actttttttc cttcagtgct atttggcgta gttttttctg caaacaatct tgataataca
    12001 cgagccacat taccatcaac agcaaagata ggttcattaa atgccattga agcaatagct
    12061 gctgcagtat aatcgcctac accttttagt tttttaagct caaaataatt ggaaggaaat
    12121 ttaccgtgaa atattttggt aatatgttgt gctgtggcta aaagatttcg ccctcttgaa
    12181 taatatccta atccctgcca caatttgagc acctcggttt ctgatgcttt ggcaagatgc
    12241 ttcactgtag ggaacgattc taaaaaccgt ttgtaataag gcaatccctg ttctactctt
    12301 gtctgctgaa ggataacttc gctcaaccat attctatagg catcgtttgt actgcgaaaa
    12361 ggcaactcgc gcttgttgcg ttcataccat aaaataagtt tgtttgaaat actcatttgc
    12421 tttggcaaag aaagtcatta taaaaaataa ttacagttta aatttaatag acaattcagt
    12481 aacaactaaa tttacttatc attgccatcg ctttttgtta tgaaattaat taaagaaata
    12541 cactctcttt ctcatattgt tagcagaaag aagcacgaga atatcagcat tggttttgtg
    12601 ccaacaatgg gcgcattaca cgaaggacat atttcattaa ctaaacagtc gctgcttgaa
    12661 aatgaattta ctattgccag catttttgta aatccattac agtttaataa tcctgatgat
    12721 ttaaaaaagt atcctcgtac cttagataga gatatgcaga tgcttgaagc ggcaggttgt
    12781 aatgccttgt ttgcaccgga agcatcagaa gtttatattg aagatgaaca accattgcag
    12841 tttgatttag gcaatcttgc aaacgttatg gaaggcaaat ttcgtccggg acattttgcc
    12901 ggagttatca ctattgttca caaacttttt tcatgggtta aacctgatgt tgcttatttc
    12961 ggagagaaag attttcagca gcttgccgtt atcagaaaaa tggctaaagc attacatcct
    13021 gatattattg tcacaggctg ccctacactt cgtgagccta atggtcttgc catgagttcg
    13081 cgcaatgtaa gattaagtaa taccgttcgt catgatgcat ccataattta taaggcaatg
    13141 caacatgctg cgcaactgaa aaacatactg cccgtttacg aagtttgttc gcaggtgaga
    13201 agtatgattg aagaaatagc tccttttaaa gttgagtatg ttgaaattgc tgatgcagta
    13261 aacctacagt ctttacaaca atggtcagac tcacagtcac cacgtatttt tgttgcagtt
    13321 tttgcagatg atgtaaggct gattgataat gcagcattgt aaataactac accgtacaat
    13381 gccgtgcaaa gacacctaat ggaagtctag ctccggcagt gctttgtaga aaaatttctc
    13441 ctgtctgtcg tttaatattt ttactaaaat gttcgtcaag cagattatct aaaattatag
    13501 atgaaaaacc aagtgaatag gtgttgagta caagaaagtg attttcttca agcagcaact
    13561 taacgttggc aagcatttcg tttatctcat cttctaattt ccagttttcg ccatttggtc
    13621 catggccata agcaggtgga tctaaaataa ttccctgata ttttttcccg cgttttactt
    13681 ctcgtttcac aaaggtcatg gcatcttcaa ccaaccagcg aatgttgtcc aagccactca
    13741 atgtcatatt ttcttttgcc catgttacaa cctgttttat agagtctaaa tgaatgatgt
    13801 cggcaccggc agctttggca gcaagtgttg atgcaccggt gtaggcaaag aggttgagga
    13861 cttttggttg tactacatct aattttttaa ctgaattata aataaaatcc cagtttacgg
    13921 cctgttccgg aaaaacacct acatgcttaa atgatgttaa tgccagccta agtcgaattt
    13981 gtttttcatc aggcaatgca taggcaatat gccaacggtc aggaatctct ttaagttttt
    14041 tccatgtacc tgacgagctg tttttagcta taaactttac atgtgctttt ttaagccaat
    14101 cggcctcttt tagttgcctg ttccacacag cttgtggttc gggcctgatg gtaatgtact
    14161 gaccaaaacg ctctaacttt tcaaagtttc cgctatcaag cagttcataa tctttaaaat
    14221 tttgcggtgt aagtaattcc atgcagaagt tcatttaaat acctaaaagt gttgtaatag
    14281 caaccttttt aatcgggctg cgtaaaaaca aggcaatatt aagtctaaaa aatgataatc
    14341 aacacatcag agctgtcggg tcgtagcata caagccacag acaaaacacc attaagaatt
    14401 ctattagttg atgatattcc ggctaattta ttgacacttg aaggtattct tgaagatgat
    14461 agccgaattc ttataaaagc tttttcgggt aatgaagctt taaaattact gatggaagaa
    14521 ccaattgatt tgatattatt agacattcaa atgcctgaca tggatggcgt tgaggtagca
    14581 caattacttc gtgccaatcc taaaacaaag catataccca tcatttttgt ttctgccatt
    14641 gccaaaagcg aacgcccttc actcgaggag tttgaaatcg gatcgtttga ttttattccg
    14701 aagccattag atattgaaga gacacagaaa atagtttcaa tctttgaatt tggatgtcgc
    14761 tataaacaaa agtacagaga acaggaagaa aaactgaaaa gactgaacga tcatcttgat
    14821 aagtttcttt acatcctttc gcatgatttg aatgcaccac tgcgtgccat tgagaatctt
    14881 gtgcagtggc ttgaagaaga tttacaatcg cagatttctg atgagagtaa ggaaaacata
    14941 gggctgctta gaaacagagt aactaagatg aaattacttc ttgatggctt attgcgttat
    15001 gcgcgttccg gtaatacaaa acatcagcac gatattattt cacctgaaaa aatgatcaga
    15061 caaattacat ctgagctgca gggtgataga aaggtgtcac tgaatgtcaa ctcttcggtt
    15121 ttagatatga atacagacag gacagcattg caacttgtgt tgaaagagtt aattcaaaat
    15181 gcaatatctt ttaatccttc cgataaaaat actattgata tttctgttgt agaaaaaaat
    15241 gattcttatg aatttgttat agcagataac ggacccggaa taaagccaca acaacaaaaa
    15301 acaattttcg aactttttca aacgcttcca tcatcggtag tgccttcatc aggtgtaggc
    15361 cttagcattg tgaaaaaggt ggttgaagaa tatggtggaa cggtgtggtt ttcaccaaca
    15421 gtaaaatgtg gtactgaagt tcactttaac tggaaaaaat cctgagcttt agaaccctga
    15481 ttgccgtgct tttattgtac ttgcaccaac tgtaaaaagt acaagactga ttgtgctaat
    15541 aggcattaat attgctgcaa acaatggcga aagtgttcca cttacggcaa aggctaatcc
    15601 aacaaaatta tagagtaaag aaataataaa gctgatgata attatcgtta cagcagattt
    15661 ggatagttta attaattcag gcagttgatg taatgctgca gcatccataa tagtatcact
    15721 tgcaggagta aaattattag tgttgtcggt aactgaaatt cccgtattgg ctgctagcag
    15781 cgcacctgca tcattcaggc catcaccaat cattaacaca tttttcttac tattgattaa
    15841 ttcaagaatg taatttcttt tgtcttcagg tttttgatta aatcgtaatt cggcattgcc
    15901 taaaagtgta gttagatttt ttctttcact ttcattatca cctgtaagaa caatcaaatt
    15961 atgtttgtta ttcagttttt gtgatatggt gttcaaccca tcacgataaa tattactgaa
    16021 aacaaatttg ccttttttaa tgtcattaat gttgataaaa actgctgttg cattcttttc
    16081 aacttcattg tttactcctg cccagtctga tgttccggct ttaatgtttt tattgtttaa
    16141 tactgcgcaa atgcctttac cggtaacttc ttcaaaatta ttgcatggat aatatttatc
    16201 tgcttgcaaa tgttgttgca gtaatttact taacggatgt gatgattgcc tgaatagcga
    16261 tgatagtatt tttttatcct catcatcaag cgattgacca tgataactta tttcagaaac
    16321 attattctta gttaatgttc ctgtcttatc aaatacaata gtgtcggcat cagcaatttt
    16381 ttcgagtact cgatagttac gcatgtagat tttatttctg ccaagaatgc ggaggatgtt
    16441 tccataagta aaaggtgcag ataaagccag tgcacaggga catgcaatta caagcactgc
    16501 tgtaaatgca ttaatagctt tatgaatatc tgtattccac caatagatag cagcgattgt
    16561 agaaatagaa aatgtaacta ctataaacca acgactgatg gcattcacta atttttcaaa
    16621 cttgcttata tcttgtttat cccagctacg attccacaat tgtactaaat agctctgcga
    16681 aacttctttc atcacctgaa gttcaatcat ggcaccggct tgtttgcctc ctgcataaat
    16741 atattcaccg gctgtatgtg aaacaggtag tgactcacct gttacaaaat gataatctac
    16801 tgaggcattg cctttaagta aaatacaatc tgcgggaata atttcattat ttcttataag
    16861 aattctgtca ttaactttca agtcattaat ctgcacttct ttttcatttc cgttttttat
    16921 agaagtaact gcaatgggaa agtatgattt aaaatctctg tcgaaagcta aactatcaaa
    16981 ggtaaaatcc tgaaaccaac gaccaatgag catgaagaaa acaagaccac taccgctgtc
    17041 aaaaaatcct gaacctgttt gtgttattat ttcaaatgca cttcgtaaaa aaattactga
    17101 tatacctatg gcaatcggca aatctatgga agtggttttt tgtttgagag cctgccaaga
    17161 tttaataaag aactcatttg cactgtaaaa aaatacgggt agtgcaagca acaaaatcag
    17221 atagctgaat agtgtttgta atgaagcatc aatattattt cctgatgaaa aatattcagg
    17281 aaaacttagc atcataatat ttccagaaca aaaaccggca acaccaattc tgagaatacg
    17341 gcttctattt gcttttttta tttttttctt ttcaatagcg tcaagttgca gtgcaggctc
    17401 atagcctgtt tgcgaaagtt tttctgcaat ttttcgaagc gaagttttat gatgatcata
    17461 aaccaaacgc aattcttttc gaacaaaatc aactcttgag gatagaacac cttcatcggc
    17521 tttctgaaaa ttttcaagta gccagataca actgctacaa tgcattgcag gaatgtaaaa
    17581 ggtgatgata gtttcatttt tatctctgaa acttatcagt tgattttcta tggaaggttc
    17641 atctaagaag gcaaatcgtt tttgaacaat gtcagactgt ttagtgccag gattgttgtt
    17701 caggttgtaa taattacaca aattattttc acgcagaatt tcataaacca acttacaacc
    17761 ggtacaacaa aaaattttct catcgcttgt gataacctgt tcatcacagt catcaccaca
    17821 atgatagcat ttcaccttat ttacagtatc tgtattgctc atagcctcca acctaatttg
    17881 cctgacaata ttacaattga tgaagaatgt agctgttgat attaattctt gatatgagtg
    17941 ataaaagtca tttcagttgt tgtctcgaaa taaaacattt gccacattca acttactatg
    18001 caaattaaac aattttataa tcagggtcat tcgcaattca gctatattat ttcaagcgga
    18061 aaagaagcgg taattattga tcctgtcaga gatgtggagc actatttaca ataccttgca
    18121 gataagcagc ttattttaaa atatattttt gaaacgcaca tacatgccga ttttgtttca
    18181 ggtcacctgg agcttgccga taaaaccgga gcaaccattg tttatggacc tcaggtgcgt
    18241 gtggcttatc cttcatatag tgcaagacat aatgaaagtt ttaatcttga aggatgtagt
    18301 attaaagctt tacatacacc gggacatact atggaatcgg tttgttattt attgaataat
    18361 aatgttgggc agcctattgc aattttcacc ggtgatactt tatttgtagg tgatgtaggt
    18421 cgtcctgatt taatgagcgg taataaaaat gcagtagaat tgtcagagta tttatatcac
    18481 tctattaacg acatcattaa aacattacct gatgaacttg ttgtttatcc gggacatggt
    18541 gccggttcgt tctgtggaaa aaatccgggt aaagaaaagt tttctaccat tggcgaacaa
    18601 aagaagagaa actatgcttt aaaggatatt tcactcgaga agtttaccaa tgaaataact
    18661 acaggcctta gtgtgccacc tgaatatttt tttaaggtta tgagcattaa tattatgggt
    18721 tgccgcccta tgaaagatgt gtttcgcaac aatctgaatt ttctgtcacc ggtaaatttt
    18781 aaagcagaaa cacaacgtgc attgatagtg gacacaagaa atgaattgga gttttcgaaa
    18841 gcatttatta aaggctctgt aaacattggt cttgatggtt cgtttgcaac atgggcaggg
    18901 attttgctcg atcccgacca tccggtttta ttggtctgcg acagtggtaa ggaaaaagaa
    18961 gccattggtg ctttagcaca gataggaata gaaaatgtga gaggtatttt acatggagga
    19021 ataaagtcat ggaaagatgc agggcaaccg gtggacaatg ttgaaaatat tgatgcagaa
    19081 gatttttatt ttttgtttga aaatgaaggt tacattctgg ttgatttaag aagccatcag
    19141 aattttaatg cacaacatat aaaaagaagt ctgagtattc cattagatga attgccacac
    19201 cgtttgaatg agtttagccc ggacactaaa tatttgttag tgtgtaaggg tggttaccat
    19261 agtatgatag cagcatcaat tcttaaacgc aatgggataa atcaggtggt aaatatttca
    19321 ggagggtttg atgcagtaag tgcatttcaa ccacagtatg tagaatcgat atagtgtgaa
    19381 cagaaggtta ctgacaattt aaacagtatt aggtataaaa caggcatgac cttaaattta
    19441 tgacaaaagt cgctttatgg gttgcttaaa gtcattttta ggttataata aaggaaatac
    19501 ttttacatcg ttaattaaaa ggaaagcaat aaaattccgg tttggaaata ccctccggtt
    19561 taaaataaaa agatttgatt tagtgttagg gttgcaaaac cggtatgctt cggctaccgg
    19621 tttttgctta ataggattat cgtaaaaagc ataagtatga aaaaaattgt tgttactact
    19681 gatttttcgg agtgttctga aaatgctttg atttattcct tgtcactgtg caaagaggtg
    19741 aatgcatcat tgtatttact tcacgttttt catataccag ttccagtgac cgacatgcca
    19801 tatatttctg tttcgtctga ggaggaggaa aagaatattg aattacgttt gaaagaattg
    19861 attacccgca taagacataa aacaggttat gagggtatga tacacaccat aaccgatttt
    19921 aattttacgg ttgatggtat tctgaatgtt gttaatgata caaatgcaga cttgctggtg
    19981 atgggacaac gaggtaaaac aacattggcg gacagactgt tcggaagtac tgcaacagca
    20041 attattaatc ttgcaacttg ccctttatta cttatacctg aaggatgcag tttcagtaaa
    20101 attaaaaaga ttgcaattgc ctgcgataaa aaaacgagtg acaataaaaa caaagtattt
    20161 catgttgttg aaatgaatgc tgcattgaag tcggaaatgc tactgtttaa tgttgttcag
    20221 gatgaagcgg aaaaggatag acttaaagga gaagatttta ctgaaatggc tggaaaagag
    20281 gtgaaattat ttttccctgt acatcacgac atacatgaag gaattttgca atttgtaaat
    20341 gataatgatg ctgattggtt gtgtatgttt ccacatcgtc ataacatttt cgagcagtgg
    20401 tttaccagac ttaattcagg taacatttcg cagcaagtaa aaattccttt attattaatt
    20461 aacgagttgg tacagtaaaa atattgctaa aaagtacttt tatgaatacg atgattttaa
    20521 atcttataaa gaagcggtta cgattcaatt taaattatga agcataggtt gactattcag
    20581 gcagaggata ctattttgga ttttcaaaaa aaattcaaca ggatttatcc gtttcttaaa
    20641 attgaatttt ttgaaaaagc acatatagcc aagcagggaa gtcataaaaa ggaaatgatt
    20701 gcttcaaaca tgttgtttcg cggattgata ggacgcggaa aatcacatat aataaacttc
    20761 aatgacgatt ttagtattag cgattttgaa atgttactga atgatcagtg tcggttatat
    20821 gcacaagtat ttcgtaaatc aggaaagatt tggcttgaaa cttcagcaac agattattgg
    20881 acattggagc aacaaaacga agaaggcaaa agcttagcca agcactttaa aacagagtca
    20941 gaaaacataa atgatattga cccttactaa ttacttcttc tttctacata ggtagaaatt
    21001 ttactaagta atagcttaat aacaaatgtg aatacaacaa tctcatatta tttgagaata
    21061 tgacttgact gaattattgg aaataaaatt caggagattc ttcaaatcct taaatctctt
    21121 cagccgcggc tgagtcgtgg ctgatcaaca atacaaaaat tttctacctc ttaacaaact
    21181 tcttgactac cacaccttgt gtgctgtatg ctttcacaaa ataaactccg ttttgaaatt
    21241 gactgatgtt tatctttaaa ttttgttggt tgttggtttt cttttcaaac aaaatttttc
    21301 caagtacgtc tattacttgt attttttcaa ctgctgttgt gagttgaata tttaattctg
    21361 attgagcagg atttgggaac aattgtatgt tttctgcact ttgatatgac gctattcctg
    21421 ttggtagcac aacatgcgtt gttgctgtgt tggttattac agggtcatta aaatcaaaat
    21481 aaa
//
LOCUS       DAOOOI010000038        21477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_471_length_21477_cov_41.511997, whole genome shotgun sequence.
ACCESSION   DAOOOI010000038 DAOOOI010000000
VERSION     DAOOOI010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 21477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21477
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_471_length_21477_cov_41.511997"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /gene="tuf"
                     /locus_tag="PKX59_06335"
     CDS             <1..968
                     /gene="tuf"
                     /locus_tag="PKX59_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOZ90522.1"
                     /translation="RHYAHVDCPGHADYVKNMVTGAAQMDGAILVVAATDGPMPQTRE
                     HILLARQVGVPKIVVFMNKVDMVDDPELLDLVEMEIRELLSFYEFDGNNTPIIRGSAL
                     GGLNADAKCVDKIMELMAAVDTYIPIPPRQVDLPFLMPVEDVFSITGRGTVATGRIER
                     GVINSNDTVEIIGMQDKKLTSTVTGVEMFRKILDRGEAGDNVGLLLRGIEKTDIRRGM
                     VVAKPGSITPHTEFKAEIYVLKKEEGGRHTPFHNKYRPQFYLRTTDVTGEIVLPEGRE
                     MVMPGDNVTITVKLIVPVAMDKGLRFAIREGGRTVGAGQVTEIIK"
     gene            1070..1140
                     /locus_tag="PKX59_06340"
     tRNA            1070..1140
                     /locus_tag="PKX59_06340"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1102..1104,aa:Trp,seq:cca)
     gene            1243..1434
                     /gene="secE"
                     /locus_tag="PKX59_06345"
     CDS             1243..1434
                     /gene="secE"
                     /locus_tag="PKX59_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOZ90523.1"
                     /translation="MNKIRAYVRESYLELTTKVTWPTWAELQNSAIIVLVASLIIGLI
                     IFFMDFLSQGIMNILYKMF"
     gene            1446..1991
                     /gene="nusG"
                     /locus_tag="PKX59_06350"
     CDS             1446..1991
                     /gene="nusG"
                     /locus_tag="PKX59_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOZ90524.1"
                     /translation="MTDTGKKWYVIRAISGKEKKVKQYIESEISRLGLSDYVAQVLIP
                     TEKVYQIKDGKKISKERSYLPGYVLIEANLVGEIPHIIENVTGVISFLGSKGEAPAPL
                     RQSEVNRILGKVDELADSEEVLNIPFVVGETVKVTDGPFNSFSGVIEEVHEDKKKLKV
                     MVKIFGRKTPLELSYMQVEKE"
     gene            2091..2528
                     /gene="rplK"
                     /locus_tag="PKX59_06355"
     CDS             2091..2528
                     /gene="rplK"
                     /locus_tag="PKX59_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOZ90525.1"
                     /translation="MAKEIGALIKLQVKGGAANPAPPIGPALGAKGVNIMEFCKQFNA
                     RTQDQAGKVLPVIITVYTDKSFEFIIKRPPVSIQLMEAAKIKTGSAESNRKKAGSVKW
                     EQVKAIAEDKMPDLNCFTIESAMSMVAGTARSLGITIEGQKPY"
     gene            2634..3332
                     /gene="rplA"
                     /locus_tag="PKX59_06360"
     CDS             2634..3332
                     /gene="rplA"
                     /locus_tag="PKX59_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOZ90526.1"
                     /translation="MAKLTKNQKLVNAKYDSEKSYTLQDASKIVKDITFTKFDASVDL
                     SIRLGVDPRKANQMVRGIVTLPHGTGKTVKVLVLCTPDKEAEAKAAGADYVGLDEYIT
                     KIEQGWVDMDVVITMPAVMAKVGKLGRVLGPRNLMPNPKTGTVTTDVGKAVTEVKGGK
                     IDFKVDKTGIIHASVGKVSFEPNKLFENANELLQSIIKLKPSSAKGTYLRSVSMSSTM
                     SPGVKVDTKSIAGQ"
     gene            3355..3879
                     /gene="rplJ"
                     /locus_tag="PKX59_06365"
     CDS             3355..3879
                     /gene="rplJ"
                     /locus_tag="PKX59_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOZ90527.1"
                     /translation="MTREEKNIEIDSLAQQFSEFPNFYITNVESLNSDKTSKLRRLCY
                     SRNVKLRVAKNNLIKKALQKLDPTAYDTLIPALKGTSAIMFSEVSNVPAKLIKEFRAK
                     DSVPAIKAAWIDSSIFVGDNQLEVLANLKSKNELIAEVIALLQSPAKNVVSQLTSGGS
                     KLAGILKTLEEKGS"
     gene            3950..4327
                     /gene="rplL"
                     /locus_tag="PKX59_06370"
     CDS             3950..4327
                     /gene="rplL"
                     /locus_tag="PKX59_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOZ90528.1"
                     /translation="MADLNAFAEQLVNLTVKEVQELSTILKDKYGIEPAAAAPVMMAG
                     GGGGGAAAAEEKTSFDVILKSGGNAKLAVVKLVKELSGLGLKEAKDLVDGAPKPVKEG
                     VSKEEANSIKQQLEEAGAEVEIK"
     gene            4453..8280
                     /gene="rpoB"
                     /locus_tag="PKX59_06375"
     CDS             4453..8280
                     /gene="rpoB"
                     /locus_tag="PKX59_06375"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOZ90529.1"
                     /translation="MAAITSTPDKGGSKRINFSKNKNLIDYPDFLDIQLKSFQDFFQI
                     ETTSDNRQNEGLYKVFAENFPITDSRNNFVLEFLDYFIDPPRYSIQECLERGLTHSVP
                     LKAKLKLYCTDPEHEDFETIVQDVYLGTIPYMTPKGTFVINGAERVIVSQLHRSPGVF
                     FGQSRHANGTKLYSARVIPFKGSWIEFATDINSVMYAYIDRKKKFPVTTLLRAIGFES
                     DKQILEIFGLADEIKVSKSGLKSHVGRKLAARVLKSWVEDFVDEDTGEVVSIERNEII
                     LERETFLEDHHIDLIIEAGVKSIILHKEDGNSADYAIIYNTLQKDTSNSEKEAVEHIY
                     RQLRNAEPPDEETARGIIEKLFFSDKRYDLGEVGRYRINKKLNLNTPEDVKVLTKEDI
                     ILIIKYLIQLINSKADVDDIDHLSNRRVRTVGEQLYSQFGVGLSRMARTIRERMNVRD
                     NEVFTPADLINAKTLSSVINSFFGTNQLSQFMDQTNPLAEITHKRRLSALGPGGLSRE
                     RAGFEVRDVHYTHYGRLCTIETPEGPNIGLISSLCVFAKINNLGFIETPYRVIENGKV
                     DLNRPPVFLTAEEEDNKVIAQANAHIDATGNFLDARVKARYEGDFPEVDPEHLHLMDV
                     APNQIASIAASLIPFLEHDDANRALMGSNMQRQAVPLMRPEAPVVGTGLEGQVVRDSR
                     VLINAQHDGVVEYVDANEIRVRSILSEDEKLLSFSDEIEVYNLIKFQKTNQSTCINLK
                     PIVRKGEKVRAGQVLCEGYATKDGELALGRNLKVAFMPWKGNNFEDAIVISERVVRED
                     IFTSLHVDEYTLEVRDTKRGLEELTADIPNVSEEATRDLDDNGLIRVGAEVKEGDIII
                     GKITPKGETDPSPEEKLLRAIFGDKAGDVKDASLKLPPSVKGVVIDKKLFSRAVKDKN
                     SKTDDKVVLTKLDDEQARDLSAIKAKLVDKLFILVNGKTSQGVMDIFKEFVIPKGSKF
                     TQKMLQELDYNNINPSKWTTDKDRNDQIKELLHNYNIKVNDVVGSYKRKKFAIQVGDE
                     LPAGIMQLAKVYIAKKRKLTVGDKMAGRHGNKGIVAKIVREEEMPFLEDGTPVDIVLN
                     PLGVPSRMNLGQIYETVLGWAGQKLGVKFATPIFDGATLDEIEHYVDKAGLSKYGATY
                     LTDGGTGERFDQPATVGIIYMLKLGHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQR
                     FGEMEVWALEAFGASNILQELLTVKSDDVVGRAKAYEAIVKGDNMPTPGVPESFNVLL
                     HELRGLGLKITLD"
     gene            8350..12645
                     /gene="rpoC"
                     /locus_tag="PKX59_06380"
     CDS             8350..12645
                     /gene="rpoC"
                     /locus_tag="PKX59_06380"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOZ90530.1"
                     /translation="MAAKRDNKIKSNFSRIIISLASPEHILEQSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDWECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIQ
                     LVVPVAHIWYFRSLPNKIGYLLGLPTKKLDLIIYYERYVVIQAGIKAQDGVNYLDFLT
                     EEEYLNILDNLPKENQHLDDTDPNKFIARMGADALYDLLSRLDLDELSYKLRHQASNE
                     TSQQRKNEALKRLNVVEAFRNANQNIENRPEWMIVKVVPVIPPELRPLVPLDGGRFAT
                     SDLNDLYRRVIIRNNRLKRLLEIKAPDVILRNEKRMLQEAVDSLFDNSRKVNAVKTES
                     NRALKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPEMKLHECGLPKDMAAELFK
                     PFIIRKLIERGIVKTVKSAKKIVDRKDAIVWDILENVLKGHPVLLNRAPTLHRLGIQA
                     FQPKLIEGKAIQLHPLVCTAFNADFDGDQMAVHVPLGNAAILEAQMLMLASHNILNPA
                     NGAPITVPSQDMVLGLYYMTKGKKSTDKEKVKGEGSVFYSPEEVTIAFNEGRVDLHAY
                     IRVKTFIKENDEIVKRVIETTVGRILFNEVVPRQAGYINDVLTKKTLRDIISQVLKTC
                     GIAQTARFLDDIKDLGFKMAFKGGLSFNLNDVLIPGAKAELVTKAQSEVEEVRNSYNM
                     GFITNNERYNQIIDIWTRTNTRITETLMKQLIADKQGFNAVYMMLDSGARGSKEQIRQ
                     LGGMRGLMAKPQKSGASGGEIIENPILSNFKEGLSILEYFISTHGARKGLADTALKTA
                     DAGYLTRRLVDVAQDVIITEPDCGTLRGLSETALKNQEEVVETLYDRILGRVSCHDIY
                     HPLTGELVVRAGDEIKEDVATQIEESPLESVEIRSVLTCESKQGVCAKCYGRNLATGR
                     MVQKGEAVGVIAAQSIGEPGTQLTLRTFHVGGVASKGASESRHEAKFGGVIELEEMRT
                     LPYKEGDNKYEVVVGRSGEFRIVDPTTKAILATNNIPYGSHLFVKNGDTLKKGDVICE
                     WDPYNAVIICEADGKIGYEHLEEGVTFREESDEQTGYKEKVVIDSRDKTKNPVIKILN
                     DKKEVVKAYNIPVGAHIVVNEGQKIKAGNVLVKIVRASGKTGDITGGLPRVTELFEAR
                     NPSNPAVVSEIDGVVAYGGIKRGNREIIITSKEGDVKKYLVPLSKHILVQDNDFIRAG
                     APLSDGAISPSDILAIKGPSQVQEYLVNEVQEVYRLQGVKINDKHFEVIVRQMMRKVV
                     IEDAGDTRFLERESVNKIEFMEENDWIMDKMVVTEPGDSEYRPGQIITLRKLRDDNSM
                     LKRKDQKEIEARNAIPATSSPLLQGITQASLHTKSWISAASFQETTKVLNEAAINGKV
                     DYLNGLKENVIVGHLIPAGTGLREYERLIVGSQEEYDLLMASKESSSEQPINM"
     gene            12851..14020
                     /locus_tag="PKX59_06385"
     CDS             12851..14020
                     /locus_tag="PKX59_06385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ90531.1"
                     /translation="MLKKLPVILVFIDWYKPAYKGGGIISCCDALVEHLKDRYCFKII
                     TRNADLGSTKQLEGIKSDCWVQRTENVEVFYLSKDKIGVFNMKSLIESTPHDLLYING
                     VFSFYFSLLPLIINKWRKLQHLTLVAPMGMLCLGALKFKNIKKTLYLKTAKLLNLYSD
                     ISWHLSSEYEKNDLEKVLGSEGEFYVAPYLFNPPQTEYKTAKKENNLLRMVFISRISP
                     KKNLLACFRYLSQVDLNVKVIFDVYGPVEDESYWNECLNQMNKLGKNIQCSYRGELKA
                     EEVIKTFQSYNVSILPTFSENFGQVIPESLLAACPVIISDQTPWNKLEEHSAGWVFSL
                     KEENRFVDIICTLAKMTNEQFKVYSEGAYQYAKGFLDSEQLVLESVRMFDAKLNQ"
     gene            14166..14792
                     /locus_tag="PKX59_06390"
     CDS             14166..14792
                     /locus_tag="PKX59_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOZ90532.1"
                     /translation="MELKKIFENNQKWIDRKLATDAQYFEKLCKGQSPEVLFVGCSDS
                     RVTAEELMGSQPGEVFVHRNVSNMIISIDLNMNAVVDYAVNFLKVKHIVICGHYFCGG
                     VKAAMQAQDLGILNPWLRNIRDVYRIHKIELNNIPDENDRYNRLVELNVQEQCINLIK
                     IASVQKAYKQRNLQVHGWVFDMQTGKLIDLKIDLPKELKDIMEIYDLG"
     gene            complement(14933..15006)
                     /locus_tag="PKX59_06395"
     tRNA            complement(14933..15006)
                     /locus_tag="PKX59_06395"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14970..14972),aa:Arg,seq:acg)
     gene            complement(15058..16029)
                     /gene="floA"
                     /locus_tag="PKX59_06400"
     CDS             complement(15058..16029)
                     /gene="floA"
                     /locus_tag="PKX59_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681040.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOZ90533.1"
                     /translation="MASIPLLILVVAGIILFFLFLYFVPVNLWITATFSGVKVSILNL
                     ILMRFRKVPPSVIVNAMITATKAGLSVTIPDLETHYLAGGNVNNVIKALISADKANIP
                     LSFKMAAAIDLAGRDVADAVQLSVNPRVIDTPPVSAVGKNGIQLVVRARVTVRTNINQ
                     LVGGAGEETILARVGEGIVTTIGSAHDHKSVLENPDQISKTVLNKGLDAGTAFEILSI
                     DIADIDVGENIGAKLQMEQASADLKVAQAMAEERRAAAVALEQEMIAKAQEARAKVIE
                     AEAQIPMAMAEAFRSGKLGIMDYYKMENIQADTSMRESIGKPPKGNS"
     gene            complement(16048..16512)
                     /locus_tag="PKX59_06405"
     CDS             complement(16048..16512)
                     /locus_tag="PKX59_06405"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOZ90534.1"
                     /translation="MGTLFTIILLILAGIVLLILEILILPGLIAGIIGALMILGGIWY
                     SFHELGNTAGTIVALSTLTVTLSTIYLAFRYNVWRRFSLQQSSDSKIERVDEMDIKSG
                     DHGKTLSALRPMGTAVFHNKKVEVQSLGEMIEANATVEVIEVLANKLIVKKI"
     gene            complement(16612..17403)
                     /locus_tag="PKX59_06410"
     CDS             complement(16612..17403)
                     /locus_tag="PKX59_06410"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOZ90535.1"
                     /translation="MEFIYSQRWCELVFENRNKNYGAYHLRQNESSRILHSWLIAITI
                     IATAIFGIAISGGPSVELIQQYSDKTQQSEIIYNFNKVTPPPLPSASTSIAVKSNGNG
                     IPVVTDRKIQTIEPLITTPVTTVPTLNSTGSGTSIVPFSSGSGTTDAGTTVTTTSDFT
                     LMPEVFPEFPGGEEALIRYVQKNIIFPHDLIRRDISGTVYVGFIIDKSGHVSNIKILK
                     GIDNAPELSVEAIRVLEKSPVWKPGLQGGKPVAVAYTLPVTFTTK"
     gene            complement(17562..18515)
                     /locus_tag="PKX59_06415"
     CDS             complement(17562..18515)
                     /locus_tag="PKX59_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOZ90536.1"
                     /translation="MSFVLKTNQAAYLLDPDNDSLVLKRKGKWSSVYLGYRVEDKLPV
                     VIKKLHNPNEMQTQLRFQREALMGFNFDGVQHTLDAWKDESGYYIIKEFIDGLSLRQL
                     MQHDIKNRTQFSIKCILATLDILEKIHSSGIIHADLRPDNILILVNKRGNPDLLNPSI
                     RIIDFGLSINMSEPVATQRIPFSLLYSPPEQLLNLPELINPSTDLFSLGLTLYESVAK
                     YPPFFNENPEMIMHMQLNTAVAEHTRIHPLLLAFIRKATFKKQLRLPPSQLSIDEIRQ
                     TVAEGQQDRFTDCNQMKTILQEVAPNISETENKSFLKRLFN"
     gene            18576..19430
                     /locus_tag="PKX59_06420"
     CDS             18576..19430
                     /locus_tag="PKX59_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HOZ90537.1"
                     /translation="MKDALKLAFSPCPNDTFMFHAWVEGLIDTQGHQFEIKMADVEEL
                     NQSAMKEDADITKLSFSAFAHVADRYELLTSGAALGHNCGPLLVSKHNFKWEELSQVK
                     IVIPGKYTTANLLLTMFAPDAKNKKELVFSDIESEVMNERADAGLIIHESRFTYHQHG
                     LKKIADLGELWENKYKCPLPLGCIAVKKSLPPEVKIQLNRLMHDSIAYAMKYPKASAD
                     FIREHAQEMDENVQQQHISLYVNQWSLELGEIGKKAIRRMMEEGTIAGLLPNTEVVFV
                     DELKKLVN"
     gene            19434..20399
                     /gene="rfaD"
                     /locus_tag="PKX59_06425"
     CDS             19434..20399
                     /gene="rfaD"
                     /locus_tag="PKX59_06425"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOZ90538.1"
                     /translation="MIVVTGAAGFVGSCLVKKLNEENFNDLILVDDFSNEHKNRNLEG
                     KKFTGKVDRRQFFKWLDENHQLTQFIFHIGARTDTTEFNKKIFDELNVEYSKKVWQKC
                     VEYGLPLVYASSAATYGGGEAGYDDDENRINQLKPLNPYGDSKNEFDKWALQQKEKPY
                     FWAGLKFFNVYGPNEFHKGRMASVIFHGFNQVQKDGVIRLFKSHRPEYKDGWQLRDFI
                     YVKDVTSVCFFLMNHRKNSGIYNLGTGKARSFYDLASSTFKAMDIECNIQFVDMPIDI
                     RDKYQYFTEAKMEKLSAIGYKEPFYSLEDGVSDYVCNYLLPAKYY"
     gene            20458..20916
                     /locus_tag="PKX59_06430"
     CDS             20458..20916
                     /locus_tag="PKX59_06430"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOZ90539.1"
                     /translation="MIKFTMRVLNLKVVRYFFSAASATIVDVGIYFLAFNYLFEKKDI
                     VFSFYTFSAPTVSLMLSYSCGLLTNFFLTKTLVFKESDLKGHHQFLRFVLVAIGVLFL
                     NYLLMKFLIRQMDWYPTIARAFSAVSIGLISFVVHKSFSFRVSATEEVED"
     gene            complement(20921..>21477)
                     /locus_tag="PKX59_06435"
     CDS             complement(20921..>21477)
                     /locus_tag="PKX59_06435"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90540.1"
                     /translation="DCHGDWGGTAFIDSCSTCVGGNTGLQPCTQDCHGDWGGTAFIDS
                     CNACVGGNTGLQPCTQDCHGDWGGTAYLDSCGICVGGYTGLWDCDTINAINIITWYNE
                     INIYPNPLSGNDINIKAGNYSGNISISITDLTGKLIFTSDCYMEKGKCLVNINPMPVD
                     GLYLFKIQFNGTMKVVKRLSIFGQ"
BASE COUNT         6978 a         3630 c         4514 g         6355 t
ORIGIN      
        1 accgtcacta tgcacacgtt gactgtcccg gtcacgctga ctatgtaaag aacatggtaa
       61 caggtgctgc acagatggac ggtgccattc ttgtagttgc tgcaactgat ggaccaatgc
      121 ctcaaacgcg tgagcacatc ctgcttgcac gtcaggtagg tgtacctaag attgttgttt
      181 ttatgaataa agttgacatg gttgacgatc ctgaattgtt agaccttgtt gaaatggaaa
      241 tccgcgaact tttgtcgttc tatgaatttg atggtaacaa cacaccaatc attcgtggtt
      301 ctgcattagg tggattgaat gctgatgcta aatgtgtaga taaaattatg gaattgatgg
      361 cagcagtaga tacctacatt ccaattcctc ctcgtcaggt tgaccttcca ttccttatgc
      421 cggttgaaga cgtgttctca attacaggtc gtggaactgt tgctaccggt cgtattgaaa
      481 gaggtgttat caactcaaac gatacggttg aaattatcgg tatgcaagat aaaaagctta
      541 cttcaactgt tacaggtgtg gaaatgttcc gcaagattct tgacagaggt gaagcaggtg
      601 ataacgttgg tttgttgtta cgcggtattg aaaaaactga catccgcaga ggtatggtag
      661 ttgcaaagcc aggctcaatt actccacaca ctgagtttaa agcagaaatc tacgttctta
      721 agaaagaaga aggtggacgt cacacaccat tccataacaa ataccgtcca cagttctact
      781 tacgtacaac tgatgtaacc ggtgaaatcg tacttcctga aggacgtgag atggttatgc
      841 ctggtgataa cgttactatc acagttaaat taattgttcc tgtggcaatg gacaaaggtc
      901 ttcgtttcgc tatccgcgaa ggtggacgta ctgtaggtgc cggtcaggtt actgagataa
      961 ttaaataatt gtattggaac agtcagcccc gttatgcggg gctggcgaaa accaatacac
     1021 ggaagcaacg gctttcgcag gagaacggtt taattatcta tcataaatta cgagtgtagt
     1081 tcaagggtag aatagcggtc tccaaaaccg ttgatgggag ttcgaatctc tccactcgtg
     1141 cagaggaaga ttgaaggtaa aatagaaatt gcaatacttg acaatatgta ttgttttaaa
     1201 aatagaacag aaactggtaa agcagtttaa acaataaaac tgatgaataa aatcagagca
     1261 tacgtacggg aatcttatct tgaattaaca accaaggtaa cctggccaac atgggccgaa
     1321 ttgcaaaata gtgcaatcat tgtacttgtt gcttcgctca ttatcggact gattatattt
     1381 ttcatggact tcctttcaca aggtatcatg aatatattat ataaaatgtt ttaatcgtga
     1441 atctgatgac cgatacagga aagaagtggt atgtgattcg tgctatcagc ggcaaggaaa
     1501 agaaagtgaa gcaatatata gaatcagaaa taagtcgttt aggcctttct gattatgttg
     1561 ctcaggtgct tatcccaact gaaaaagtgt atcagattaa agacggtaaa aaaataagta
     1621 aagaaagaag ttatctgccc ggttatgtgt tgattgaagc caatcttgta ggagaaatac
     1681 ctcacattat agaaaatgta acaggcgtta tcagcttttt aggttctaaa ggcgaggcac
     1741 cagcaccatt acgtcagtcg gaagtaaaca gaatattagg taaagttgat gaactggcag
     1801 attcggaaga agtgttgaat attccgtttg tagttggaga aacagtgaaa gtaactgatg
     1861 gtccgtttaa cagcttctca ggtgtgattg aagaggttca tgaagacaag aaaaaactta
     1921 aggttatggt gaaaattttc ggcagaaaaa caccacttga gttaagttat atgcaagtag
     1981 aaaaagaata aaaatcaata agccgtgctg tgctgaaaag agtaagtgat ttagcttcct
     2041 tatcaccaac tccgaacaaa acaaacggta attaaaatca aaaaaatacc atggcaaaag
     2101 aaataggagc cctcattaaa ttgcaagtaa aaggtggagc agcaaatcct gcccctccga
     2161 ttggtcctgc attaggtgct aagggtgtta acattatgga gttttgtaag cagttcaatg
     2221 ctcgcactca ggatcaggcc ggaaaagtgt tacctgtaat tattactgta tataccgata
     2281 agtcatttga atttataatc aaacgtccac cggtatcaat acaattgatg gaagcagcca
     2341 agattaaaac tgggtctgct gaatcgaatc gtaaaaaagc tggatcggtt aagtgggaac
     2401 aagtaaaagc tattgctgaa gataaaatgc ctgacttaaa ttgctttacc attgaatctg
     2461 ctatgagcat ggttgcaggt actgcacgta gtcttggtat tacaattgaa gggcaaaagc
     2521 cttattaatt actgccggac aacaaaggtt gttcagcatg atttgaaaaa gtaaattaac
     2581 agaaggagtt tcggtttata aacggaacgt tatcacttca caaattatat aaaatggcaa
     2641 aactgacaaa aaaccagaag ttggttaatg cgaagtacga cagtgaaaaa tcatatacac
     2701 tgcaggatgc ttctaagatt gttaaagaca tcacgtttac aaagtttgat gcatcagttg
     2761 atctgagcat tagattaggc gttgatccac gtaaagcaaa tcagatggtt cgtggcatcg
     2821 ttaccctgcc gcatggaaca ggaaagactg taaaagtatt ggtgctttgc acacccgaca
     2881 aagaagcaga agcaaaagct gccggtgccg attatgttgg attggatgag tatatcacca
     2941 aaatcgaaca aggttgggtt gatatggatg tggtaattac catgcctgca gtaatggcta
     3001 aggtaggtaa attaggtcgt gtattaggtc ctcgtaacct tatgccaaat ccaaagacag
     3061 gaactgttac tactgatgta ggtaaagctg tgactgaagt taagggcggt aagattgatt
     3121 ttaaagttga caaaacagga atcattcatg catcagttgg taaagtatca tttgagccga
     3181 acaaactttt tgaaaacgcc aatgagttgc ttcagagtat tatcaaatta aaaccatcat
     3241 cagcaaaagg tacttatctg agaagtgttt caatgagcag tacaatgagt cccggtgtta
     3301 aggtggatac aaaatcaatt gcaggtcaat aatcatatat aaattattca acaaatgaca
     3361 agagaagaaa aaaatataga aatagatagc ctggcacaac agttcagcga gtttccaaac
     3421 ttctacatta ctaatgtaga gagcctgaac agtgataaaa catctaagct gcgcagactt
     3481 tgttacagca gaaatgtaaa actgcgtgta gcaaaaaata acctcatcaa gaaggcatta
     3541 caaaagttgg atccaactgc ttatgatacg ttgattcctg cattaaaagg tacttcggca
     3601 attatgttca gtgaagtatc aaacgtgcct gcaaaactta taaaagagtt cagagcaaaa
     3661 gacagcgttc ctgcaataaa agctgcatgg attgattcat caatctttgt tggcgacaat
     3721 cagttagagg tattagcaaa tctgaaatct aagaatgaac ttattgcaga ggttattgca
     3781 ttacttcagt cacctgctaa aaatgtggtt tcacaactta catctggcgg cagcaaactg
     3841 gcaggtatat taaaaacatt agaagagaaa ggttcttaag cttccacctt tgaatcttaa
     3901 actatagtac aattcaaata aacatttaaa acaattaaaa ttaaataaaa tggcagattt
     3961 aaatgcattc gcagaacagt tggtaaacct gacagtaaaa gaagttcagg aactttcaac
     4021 cattctaaaa gacaaatacg gtattgagcc tgctgctgca gcaccagtta tgatggccgg
     4081 tggcggtggc ggtggcgcag ctgctgctga agaaaaaaca tcttttgatg taattcttaa
     4141 gtcaggtggt aatgcaaaac tggcagtagt aaagctggta aaagaattga gcggacttgg
     4201 cttgaaagaa gctaaagact tagttgatgg cgctccaaaa ccggttaaag aaggtgtttc
     4261 taaggaagaa gcaaattcta tcaagcaaca acttgaagaa gccggagctg aagttgagat
     4321 caagtaatac ctcggtttaa aaaatcatag gccttttcca aatggggaag gcctataccc
     4381 cttttacagc ctctgcaatt gcacaggctt ttagtacaac aaggattgaa atcaatataa
     4441 aaacaaatac acttggctgc aatcacatca acgccagata aaggcggttc aaaaagaata
     4501 aacttctcta agaataaaaa cttaattgat tatcctgact ttctcgacat tcagttgaaa
     4561 tcgtttcagg attttttcca gatcgaaaca acatctgaca accgtcagaa tgaaggtctt
     4621 tataaagtat tcgcagaaaa ctttccgata acggattcac gaaataattt tgtgcttgag
     4681 tttctcgatt attttattga cccgccaaga tattctattc aggaatgtct tgaaagagga
     4741 ctcacacaca gtgtacctct aaaagctaaa ttaaaattgt actgtaccga ccctgagcat
     4801 gaggatttcg aaacaattgt tcaggatgtt tacttaggta caattccgta catgacacca
     4861 aagggaacct ttgtgattaa tggtgcagaa cgcgttattg tttcgcagtt gcaccgttca
     4921 ccgggagtat ttttcggaca aagccgtcat gctaacggaa caaaacttta ttcagcacgt
     4981 gtaattcctt tcaaaggatc gtggattgaa tttgcaacag acattaacag tgtcatgtat
     5041 gcgtacattg accgtaaaaa gaagtttccg gttaccacat tgttgcgtgc aataggtttt
     5101 gaaagcgata aacagattct tgaaattttc ggacttgctg acgagattaa agtaagcaaa
     5161 tcaggattaa aatcacatgt tggccgtaag ttagctgcac gtgttcttaa atcgtgggtt
     5221 gaagattttg tggatgaaga taccggtgaa gtagtttcta tcgagcgtaa tgaaattatt
     5281 cttgagcgtg aaaccttcct tgaagatcat cacattgacc tgattattga agccggagta
     5341 aaatccatta tccttcataa agaagatggc aactctgctg actatgcaat tatttacaac
     5401 acgctgcaga aagatacttc caactctgaa aaagaagcgg ttgaacatat ctatcgtcag
     5461 ttgcgtaatg cagaaccacc ggatgaggaa acagcacgtg gtatcatcga aaaacttttc
     5521 ttctctgata agagatatga tttaggtgaa gtaggtcgtt accgtatcaa taaaaaactt
     5581 aacctcaata cacctgaaga tgtgaaggtg cttactaaag aggatattat tttaatcata
     5641 aagtacctca ttcagcttat caactctaaa gctgatgtgg atgatattga tcacttatca
     5701 aacagacgtg tacgtacagt tggcgaacaa ttatattctc agtttggtgt aggtttatcg
     5761 cgtatggctc gtaccatccg tgagcgtatg aacgttcgcg acaacgaagt gtttacgcct
     5821 gctgatttga ttaatgcaaa aacattgtct tcagtaatca attcattctt cggaaccaat
     5881 cagttgtcac agtttatgga tcaaacaaat ccgttggctg agattacgca taaaagaagg
     5941 ctctctgccc ttggacccgg aggtctttcg cgcgagcgtg caggtttcga ggtacgtgac
     6001 gttcactata cacattacgg acgtttgtgt accattgaaa cacctgaagg accaaacatc
     6061 ggtttgattt cttcactctg tgttttcgca aaaatcaaca atcttggatt cattgaaaca
     6121 ccttatcgtg taatcgaaaa cggaaaagtg gatttgaatc gtcctcctgt atttttaact
     6181 gcagaagaag aagataataa agtaattgca caggcaaatg cacatattga tgcaacagga
     6241 aacttccttg atgcacgtgt taaagcacgt tatgaaggtg acttccctga agtagatcct
     6301 gagcatctgc acctgatgga cgtagcacct aatcagattg catctattgc agcatcattg
     6361 attcctttcc ttgaacatga tgatgcaaac cgtgcactga tgggttcaaa catgcaacgg
     6421 caggctgtgc cgttgatgcg cccggaagcc cctgttgttg gaacaggact tgaaggtcag
     6481 gtggtgcgcg actcacgtgt gctcatcaat gcgcaacatg atggggtagt tgaatatgta
     6541 gatgccaatg aaattcgtgt tcgcagcatt ctttcagaag atgaaaaact gttgagcttt
     6601 agtgatgaga ttgaagttta taatctgata aaattccaga agacaaatca aagcacttgt
     6661 attaacctta agcctattgt tagaaaaggg gagaaggtta gagcaggaca ggtattgtgc
     6721 gaaggttatg caaccaaaga cggtgaattg gccttaggac gtaaccttaa agttgctttc
     6781 atgccctgga aaggaaataa ctttgaagat gctattgtga tttctgagcg tgttgtacgc
     6841 gaagatattt tcacttcgct gcacgttgac gaatatactc ttgaagttcg tgacaccaag
     6901 cgtggtcttg aagaacttac tgcagatatt ccaaacgtta gtgaagaagc aacccgcgac
     6961 cttgatgata acggattgat tcgtgttggt gcagaagtga aagaaggaga tatcattatc
     7021 ggaaaaatca caccaaaagg agaaacagat ccttcacccg aagagaaact gcttcgtgcc
     7081 atctttggtg ataaagccgg tgatgttaaa gatgcatcac tgaaactgcc accatctgta
     7141 aaaggtgttg taattgacaa gaagttgttt tcacgtgcag tgaaagataa aaactcaaag
     7201 actgatgata aagtagtgtt aaccaaactt gatgatgagc aggcaagaga tctatcagct
     7261 ataaaagcta aattggttga caaactgttt atcctcgtta atggaaaaac atcgcaaggt
     7321 gtgatggata tctttaaaga atttgttatt ccgaaaggca gcaagtttac tcagaagatg
     7381 ttgcaggagt tggattataa taacatcaac ccatcaaaat ggacaaccga taaagatcgt
     7441 aatgatcaga taaaagagtt gttgcacaac tacaacatta aagtaaatga tgtggtaggt
     7501 tcctataaac gtaagaagtt tgctattcag gttggtgatg aactgccagc aggtattatg
     7561 cagttagcaa aagtttacat tgctaaaaaa cgtaaactta cagtaggtga taagatggca
     7621 ggccgtcatg gtaataaagg tattgttgct aagattgtcc gtgaagagga aatgcctttc
     7681 cttgaagacg gaacacctgt tgacattgta cttaatccgc ttggtgtacc ttcccgtatg
     7741 aaccttggtc agatttatga aactgtactt ggatgggcag gacaaaagtt aggtgtaaag
     7801 tttgcaacac caatatttga tggagcaaca ttagatgaaa ttgaacatta tgtagataaa
     7861 gcaggattat cgaaatatgg tgctacatac cttacagatg gtggcacagg agaacgcttc
     7921 gatcagcctg caacagttgg tatcatctat atgcttaagt taggtcacat ggttgatgat
     7981 aagatgcatg cccgttcaat aggaccatac tcactcatca cgcaacagcc tttaggtggt
     8041 aaagcacagt ttggtggtca gcgtttcggt gagatggagg tatgggcact tgaagcattc
     8101 ggtgcatcaa acattcttca ggaattgttg acagttaagt ctgatgacgt agttggaaga
     8161 gctaaagctt atgaagcaat tgttaaagga gataacatgc ctacaccggg agtacctgag
     8221 tcgttcaatg tattactgca tgaattgcgt ggattaggtt taaaaataac attagattaa
     8281 aagaacatgt gcagggtttt tgctctgcac ataacttttc tatcataaaa attattcaac
     8341 aaaaacaaaa tggcagcaaa acgcgataat aaaataaaaa gtaatttttc acgcatcatc
     8401 atcagcttag cttcaccgga acacattctg gagcagtcaa gcggtgaagt gctgaaacca
     8461 gaaacaatta actaccgcac gtataaacca gagcgtgatg gtttgttttg cgaacgcatt
     8521 ttcggtcctg taaaagactg ggaatgtcat tgcggcaagt acaagcgtat ccgttataaa
     8581 ggaattgttt gtgaccgttg cggtgtagaa gtaacagaaa agaaagttag acgtgagcgc
     8641 atgggacaca tacagttagt tgttcctgtt gcacacatct ggtatttccg ttctttacct
     8701 aataaaatcg gttatctatt aggattacct acaaagaaat tagatctgat tatttactac
     8761 gaacgttatg tagtaattca ggcaggtata aaagcacagg atggtgtaaa ctatcttgac
     8821 ttcctcaccg aagaagagta cctgaatatt cttgacaacc ttccaaaaga aaatcagcat
     8881 cttgacgata ctgatccgaa taaatttatt gcaagaatgg gagcagatgc tttgtatgac
     8941 ctgctttcac gcctcgacct tgatgagtta tcctataaac tacgtcatca ggcaagcaat
     9001 gaaacttcgc agcaacgtaa gaacgaagca ttaaagagac tcaacgtagt tgaagctttc
     9061 cgtaatgcga atcagaatat tgaaaaccgt cctgagtgga tgatagtaaa agttgttcct
     9121 gttattccac cggaacttcg tccattggtt ccattggatg gcggacgttt tgcaacatcc
     9181 gatctgaatg acctttacag acgtgtaatt attcgtaaca accgtttgaa gcgattgctt
     9241 gaaatcaaag ctcctgatgt gattttgcgt aatgaaaagc gtatgttgca ggaagcagta
     9301 gattctcttt tcgataattc acgcaaagtg aatgccgtta aaactgaatc aaaccgtgca
     9361 ttaaaatcac tttcagattc attaaaaggt aaacaaggac gtttccgtca gaaccttctt
     9421 ggtaaacgtg ttgattattc tgcacgttcg gtaattgttg ttggtcctga aatgaaattg
     9481 catgagtgcg gattaccgaa agatatggca gcagaattat tcaagccatt tattatccgt
     9541 aaacttattg aaagaggaat tgtaaagaca gttaagtctg ctaagaaaat tgtagatcgt
     9601 aaagatgcta tagtttggga tattcttgaa aacgtattaa aaggacatcc tgtattactt
     9661 aaccgtgctc ctacacttca ccgtctcggt attcaggcat tccagccaaa acttatcgaa
     9721 ggaaaagcaa ttcagttaca tccattggtg tgtacagcat tcaacgctga ctttgacggt
     9781 gaccagatgg ctgttcacgt tccccttgga aacgctgcaa ttcttgaagc acaaatgctg
     9841 atgcttgcat cgcacaacat tcttaaccct gccaatggtg caccaattac agttccttca
     9901 caggacatgg ttcttggctt gtattacatg actaagggta agaaatcaac agataaagaa
     9961 aaagtaaaag gagaaggttc tgttttctat tctcctgaag aggttaccat tgcgttcaat
    10021 gaaggccgtg ttgatttaca tgcatacata agagtaaaaa cattcatcaa agaaaatgat
    10081 gaaattgtaa aacgagtgat agaaaccact gttggacgta tattgttcaa cgaagtggtt
    10141 ccacgtcagg caggatatat taacgatgta cttacaaaaa agacattgcg tgatatcatc
    10201 agccaggtat tgaagacctg tggaattgcg cagacagcac gtttccttga tgatataaaa
    10261 gatcttggat ttaagatggc atttaaaggt ggtctttctt tcaaccttaa cgatgtattg
    10321 attcctggtg caaaagctga attggttact aaagcacagt cagaagtaga agaggttcgt
    10381 aacagctaca acatgggatt cattaccaat aacgagcgtt acaatcagat tattgatatc
    10441 tggacacgta ccaatacacg tattacagaa acgttgatga agcaattaat tgctgataaa
    10501 caaggtttca acgcagtgta tatgatgctt gattccggtg cgcgtggttc taaagagcag
    10561 attcgtcagt taggcggaat gaggggattg atggcaaagc cgcaaaagtc aggtgcatca
    10621 ggtggtgaga ttatcgagaa tccgattctt tcaaacttta aagaaggatt atcaattctt
    10681 gaatacttta tctcaacaca cggtgcgcgt aaaggtcttg ccgatacagc gttaaagact
    10741 gccgatgcag gttatctgac cagaaggctt gttgacgtag cacaagatgt tatcatcaca
    10801 gaacctgatt gcggaacatt acgtggtctt tctgaaacag cattgaaaaa tcaggaagaa
    10861 gttgttgaga cattatatga cagaattctt ggacgtgtat cgtgtcacga tatctatcac
    10921 ccattaaccg gtgaattggt tgttcgtgca ggtgatgaaa taaaagagga cgttgcaaca
    10981 caaatagaag aatcaccatt agaatccgta gaaatcagat cagtattgac ctgcgaatcg
    11041 aaacaaggtg tatgtgctaa atgctatggc agaaacttag ctacaggacg catggttcag
    11101 aaaggtgagg ctgttggtgt tattgcagca cagtccattg gtgagccggg tacacagctt
    11161 acacttcgta ctttccacgt gggtggtgtt gcatcaaaag gtgcatcaga gtcacgacat
    11221 gaggcgaagt ttggtggtgt tatcgaactt gaagaaatgc gtacgctgcc ttataaagaa
    11281 ggcgataata aatatgaagt tgttgttggt cgttcgggtg aattccgaat tgttgaccca
    11341 acaactaaag caatattagc aacgaacaac attccttatg gatcacatct gtttgttaaa
    11401 aatggcgaca ctttgaaaaa aggcgatgtg atttgcgaat gggatccata caatgcggta
    11461 atcatttgcg aagctgatgg taaaattggc tatgaacatc ttgaagaagg tgttacattc
    11521 cgtgaagagt ctgatgagca aacaggttac aaagagaaag ttgtcattga cagccgcgat
    11581 aaaacaaaga accctgttat taagattctt aacgacaaga aagaagttgt aaaagcatat
    11641 aacattcctg ttggtgctca cattgtggtg aatgaaggtc agaaaattaa ggcaggaaat
    11701 gtattggtga aaattgttcg tgcaagtggt aagactggtg acatcaccgg tggtttgcca
    11761 cgtgtaacag aattgtttga agcaagaaat ccatcgaatc ctgctgtagt aagtgagatt
    11821 gacggagtag tagcttatgg cggaattaaa cgtggtaacc gtgagattat catcacatct
    11881 aaagaaggag atgtaaagaa atatcttgtt ccgttatcaa aacacattct tgtacaggac
    11941 aacgacttta tccgtgcagg tgctccatta tctgatggtg caatcagccc atccgacatt
    12001 cttgcaatca aaggaccatc gcaagtacag gaatacttag taaacgaggt gcaggaagtg
    12061 tatcgtttgc agggtgtgaa gattaacgat aagcactttg aagttatcgt tcgtcagatg
    12121 atgcgtaagg tagtaattga agatgcagga gatacacgct tcctcgagag agagtctgta
    12181 aacaagattg agtttatgga ggagaacgat tggattatgg ataagatggt ggtaacagaa
    12241 cccggtgatt cagaatatcg tccaggtcag attattacat tacgtaaatt acgtgacgac
    12301 aactcaatgc ttaagcgcaa agatcaaaaa gagattgaag cacgtaatgc tattcctgct
    12361 acatcatcac cattgttgca gggtatcact caggcatcat tgcatactaa gagttggata
    12421 tcggcagcat cattccagga aacaactaaa gttcttaatg aagcagcaat taatggaaaa
    12481 gttgattatc tgaacggctt gaaagaaaac gttatcgttg gtcatttaat tcctgcagga
    12541 acagggttac gtgaatatga gcgtttgatt gttggctcac aagaagagta tgacttgctt
    12601 atggcttcaa aagaatcaag cagcgagcag cctattaata tgtaaaattt agatagagca
    12661 tatttaaaca aacacctttc agacccaagt ctgaaaggtg ttttactttt aacaggtagt
    12721 tagtttttga aattgtatgc tctaatttga ttatacaatt ttaaaatgta atagataaaa
    12781 tttttatttt cgtattttgt taacccaaat gtaaataacc attggtaggt tagttttaga
    12841 gattagaaaa atgctaaaaa aattacctgt tatccttgtg tttattgact ggtataaacc
    12901 agcatacaaa ggtggaggta taatttcctg ttgtgatgca ttagtagagc atctgaaaga
    12961 cagatattgc tttaaaataa ttacacgaaa cgcagacttg ggttccacca aacagcttga
    13021 aggaattaaa agtgattgtt gggttcaacg tactgaaaat gtagaagtat tttatctttc
    13081 aaaagataag attggtgttt tcaatatgaa atcattgatt gaaagtacgc cacatgacct
    13141 attatacatt aatggggtat tttcgtttta ttttagttta ttgcctttaa taatcaataa
    13201 gtggcgtaaa cttcaacacc taacattggt tgctccaatg gggatgttat gtttaggagc
    13261 acttaaattc aagaatatta aaaagactct ttatcttaaa acagctaagt tgcttaattt
    13321 gtattcggat atttcgtggc atctgtcatc agaatatgaa aagaatgacc ttgagaaagt
    13381 gttgggaagc gaaggagaat tttatgttgc tccctattta tttaatccgc cacaaacaga
    13441 atataaaacg gcaaagaagg aaaataattt gctgcgaatg gtttttattt cacggatatc
    13501 acccaaaaaa aatcttttag cttgctttcg ttatttatca caagttgatt taaatgtgaa
    13561 agtgattttt gatgtttatg gcccggtaga agatgaatct tattggaatg aatgtttgaa
    13621 tcagatgaat aaacttggaa aaaacattca atgtagctac cgtggagaat tgaaagctga
    13681 agaggttata aaaacatttc aaagttataa tgtttctatt ttaccaactt tcagcgaaaa
    13741 ctttgggcag gttataccgg aatctttact tgctgcatgt cctgtaatta taagcgatca
    13801 aacaccatgg aataaattgg aagagcattc tgccgggtgg gtgttttctt taaaagaaga
    13861 aaataggttt gtggatataa tatgcactct tgcgaaaatg acaaatgagc aatttaaggt
    13921 ttattcggaa ggagcttatc aatatgcaaa aggcttttta gatagtgaac aattagtgtt
    13981 ggagtctgta agaatgtttg atgctaagct gaatcaataa ttgttttcta tattttattt
    14041 ttaatgtata atgaaatata tgatgacaaa ttcatgcgtt agtttatcca gaatgatagt
    14101 tggatttatt gctgttaaaa ttcaagcaag gaggtaacaa tattttttat tattaaattt
    14161 gtacaatgga acttaaaaaa atatttgaaa ataatcagaa atggattgat aggaaactgg
    14221 ccactgatgc gcaatatttt gaaaaattgt gcaaaggtca aagtcctgaa gttttatttg
    14281 taggttgttc tgacagcagg gtaacagcag aggagttaat gggatcgcag cccggtgaag
    14341 tatttgtaca tcgcaatgtt tcaaatatga ttatcagcat tgatttaaac atgaatgctg
    14401 ttgtagatta tgctgtgaat tttttaaaag tgaaacacat cgtaatctgt ggccattatt
    14461 tttgtggagg agtaaaggct gctatgcagg cacaggattt aggcatactc aatccatggc
    14521 taagaaatat tcgtgatgta tatcgtattc ataaaataga attgaataat attcctgatg
    14581 aaaacgatcg ttataatcgc ttagtggagt tgaatgttca ggaacaatgc attaatctga
    14641 ttaaaattgc atcggttcaa aaagcatata agcaacgcaa tttgcaagta catggatggg
    14701 tgttcgatat gcaaaccggc aaattaattg acctgaaaat tgatttacca aaagagttga
    14761 aagatattat ggagatctat gatttagggt aggtagttgc cttaagaata tcaaacattc
    14821 atggtaagat ttcctcatat tttaaatttc taattttctt aactgcagta cattcagtag
    14881 ccataaatag aaaacagaaa agcgaaccat tactgattcg ctttatatta agtggtcccg
    14941 gagggattca aacccccaac cttctgatcc gtagtcagat gctctattca gttaagctac
    15001 ggaaccattt ttttagtggc gcaaagataa ccaaattagc ttattacaaa agagaaatta
    15061 tgaatttcct tttggtggct taccgattga ctcacgcata gatgtgtcgg cctgaatgtt
    15121 ttccattttg taatagtcca ttatgcctaa tttaccacta cgaaaagctt ctgccatagc
    15181 cataggaatt tgtgcttcgg cttcaataac tttggctctt gcctcttgtg ctttggcaat
    15241 catttcctgt tctaaagcta cggctgcagc cctgcgctct tctgccattg cctgtgctac
    15301 ctttaagtct gcagaagcct gttccatctg aagttttgct ccaatgtttt cacctacatc
    15361 aatatcggca atgtctatag ataaaatttc aaatgccgtg cctgcatcaa gacctttgtt
    15421 taatacagtt tttgaaatct gatcgggatt ttctaataca cttttatggt catgtgccga
    15481 accaatagta gttacaatac cttcgccaac acgtgcaaga atggtttcct cacctgcacc
    15541 accaactaac tgattgatgt tggtgcgaac ggtaacacga gcacgtacta ccaattgaat
    15601 tccgtttttg cccacagcag aaacaggagg ggtgtcaata actcgtggat ttacagaaag
    15661 ctgaacagcg tctgcaacat cgcgaccggc cagatcaatt gctgctgcca ttttaaatga
    15721 taagggaatg tttgctttat cagcagaaat tagtgcttta ataacattat ttacatttcc
    15781 tcctgccaga tagtgtgttt ctaaatcagg aatggtaaca cttagacctg ctttagttgc
    15841 agtaatcata gcattaacaa ttacacttgg tggtactttt ctgaaacgca tcaggattaa
    15901 gttcaggatg ctgactttca caccggagaa tgttgcagtt atccaaaggt taacaggaac
    15961 aaaatacaag aacaaaaaga acaggataat acctgcaaca accagaatga gtaacggaat
    16021 acttgccatt ttttttaatt tttaagttta tatttttttt acaataagtt tgtttgccaa
    16081 tacttcaatc acttcaactg tagcgtttgc ttctatcatt tcgccaagtg attgcacctc
    16141 tactttttta ttatgaaata ctgctgtgcc cataggtctc aatgcagaaa gtgtttttcc
    16201 atgatctccg gattttatat ccatttcatc aacacgctca attttagaat cacttgattg
    16261 ttgcaggctg aacctgcgcc aaacattgta acgaaatgca agataaatgg tagatagtgt
    16321 tacagtaagt gtacttagtg caacgatggt gcctgcagta tttcccagtt catgaaagga
    16381 ataccagatg ccacccaaaa tcattaatgc accaatgata cctgcaatta atcccggcag
    16441 aatcaatatc tccaaaatca acagaactat acctgcaagt atcagtaaaa tgattgtaaa
    16501 taaggttccc attttcggtg acaaacatag taaaaaggcc gttgctaaaa aacaacagcc
    16561 cctattaaaa aacacgaata ttttttatgc tgatttttgc tgaattgtaa attatttggt
    16621 agtaaatgtc accggtaatg tatatgcaac tgccacaggt ttacctcctt gtagtcccgg
    16681 tttccaaact ggagattttt caagcactct gattgcttca actgacaatt caggtgcgtt
    16741 atcaatacct ttaagaattt tgatattgct gacatgacca cttttgtcaa ttataaatcc
    16801 aacataaaca gttccactga tatcacgcct gattaggtca tgtggaaaaa taatattttt
    16861 ttgcacgtaa cgaattaagg cttcttcacc tcccggaaat tcaggaaata cttcaggcat
    16921 taatgtgaaa tctgaagttg tggtgactgt agttcctgca tctgtagtgc cactgccaga
    16981 tgaaaaggga acaatagaag tgccgctacc agttgaatta agagtgggta cggtagtaac
    17041 aggtgtggtt attaaaggtt caatagtttg aatttttcta tcagtcacta cgggaatgcc
    17101 gttaccgttg gactttactg caattgatgt agatgcagaa ggtagtggag gaggtgttac
    17161 cttgttgaag ttataaatta tttcagattg ttgagtttta tctgagtatt gctgaataag
    17221 ttcaacagat ggaccacctg atatcgcaat tccaaatatt gctgttgcaa taattgtaat
    17281 ggctatcaac caagagtgta gaatgcggct tgattcattt tgtcttaaat gatatgcacc
    17341 ataatttttg ttgcggtttt cgaacaccag ttcgcaccac cgctgtgaat aaatgaattc
    17401 cataatgttg cttttatctt ttagatgtat gacgctgcaa cattccatat tatttttagc
    17461 tgaaaactat tttacattct aatgacggta aaatttgatt ccttcttcaa gttaaaaatg
    17521 tatatattct tacattaatt tcttttaaca gaatgaaaaa atcaattaaa tagcctttta
    17581 agaaaactct tgttttcagt ttcagaaata tttggtgcta cttcctgtaa aattgttttc
    17641 atttgattgc aatctgtaaa tcggtcttgc tgaccttcag caactgtttg ccgtatttca
    17701 tcaattgata attgtgatgg aggaagacgg agttgttttt tgaaagtagc tttacgtata
    17761 aaagctaaaa gcaaagggtg tattcttgta tgctctgcaa ctgcagtatt taactgcatg
    17821 tgcattatca tctccgggtt ctcattaaaa aacggaggat acttagccac agattcatat
    17881 aatgtaagtc caaggctgaa taaatctgta gatggattta tgagttccgg taaatttaat
    17941 aactgttcag gtggtgagta gagaagtgaa aaaggtatcc gctgtgttgc aacaggctct
    18001 gacatattga tggaaagtcc gaagtcaata attcgaatac ttgggttgag taaatcagga
    18061 ttacctcttt tgttaacaag aatcagaata ttatcaggtc gtaaatcagc atgaataatt
    18121 ccgctgctat gaattttttc gagaatatcg agtgtggcaa gaatacattt gatactgaat
    18181 tgtgttctgt tttttatgtc atgttgcatc agttgacgca atgaaaggcc atcaataaat
    18241 tctttaatta tataatagcc gctttcatct ttccatgcat cgagtgtatg ctgtacacca
    18301 tcaaagttaa agcccatcaa tgcttcacgc tgaaacctta attgtgtttg catttcattc
    18361 gggttgtgta attttttaat caccaccggt aatttatctt ctacacgata acctaaatat
    18421 acagaactcc attttccttt gcgtttcagc acaaggctat cattatcggg gtcgagaagg
    18481 taggctgcct gattggtttt cagaacgaat gacatgattc agagaatatt tatggtgggc
    18541 taaattaata aacaagcttt atgtaaattt gttgcatgaa agatgcactt aaattagcat
    18601 tttctccctg tcccaatgac acctttatgt ttcatgcatg ggtagaaggt ttaattgata
    18661 ctcaaggtca tcagtttgaa attaagatgg cagatgtcga agaattaaat cagtcagcaa
    18721 tgaaagagga tgcagatatt accaaattga gttttagcgc ctttgcacat gttgcagaca
    18781 ggtatgaatt gctgacttca ggagctgcac ttggacataa ttgcggaccg cttttagtaa
    18841 gtaagcataa ttttaaatgg gaagaattat cacaagtaaa aattgttatt cccgggaagt
    18901 atacaacagc aaatttattg ctgacaatgt ttgcaccgga tgcaaaaaat aaaaaagaat
    18961 tggttttttc agatattgaa agtgaagtga tgaatgaaag agccgatgcg ggattgatca
    19021 tacatgagag tcgttttact tatcatcagc atggattgaa aaaaattgct gacttgggag
    19081 aactttggga aaataaatat aaatgcccat taccgttggg ttgtattgca gtaaaaaaga
    19141 gcctgccacc ggaagtaaaa attcaattaa atagattaat gcatgatagc attgcttatg
    19201 caatgaaata tccgaaggct tctgctgatt ttatcagaga gcatgcacag gagatggatg
    19261 agaatgtgca acagcaacat atttcattgt atgttaatca atggtcgtta gagctgggag
    19321 agataggaaa aaaggcaatc agaagaatga tggaagaagg cactattgcc ggattattac
    19381 caaatacaga agttgtattt gtagatgagt taaaaaaatt agttaattag aaaatgatag
    19441 tagttacagg agcagcagga tttgttggaa gttgtctggt aaagaaactc aacgaagaga
    19501 attttaatga tttgattctt gtagatgatt tcagcaatga acataagaat agaaatcttg
    19561 aaggaaaaaa atttacaggc aaagtagata ggaggcaatt tttcaagtgg ttagatgaaa
    19621 atcatcaact tacacagttt atttttcata taggtgcgcg gaccgataca acagaattta
    19681 acaaaaagat ttttgatgag ttaaatgttg aatacagtaa aaaggtttgg cagaagtgtg
    19741 ttgaatatgg cttgccgctt gtttatgcat catcagctgc aacttatggt gggggagaag
    19801 caggctatga tgatgatgag aatagaatca atcagttaaa accattgaat ccttatggag
    19861 attcaaagaa cgaatttgat aagtgggcac tgcaacaaaa agaaaagcct tatttctggg
    19921 cggggttaaa attttttaat gtatatgggc caaatgaatt tcataaaggc aggatggcat
    19981 cagttatttt tcatggattt aatcaggtgc aaaaagatgg tgttattcga ttatttaaat
    20041 cgcatcgccc tgagtataaa gatggctggc aattgcgtga ttttatctat gtgaaggacg
    20101 ttacatcagt ctgtttcttt ctgatgaatc atcgtaaaaa ttccggtatt tacaatcttg
    20161 gtactggaaa ggcaaggagt ttttatgatt tagcatcaag tacattcaag gcaatggata
    20221 ttgagtgtaa tattcagttt gttgatatgc caattgacat tagagataaa tatcagtatt
    20281 ttacagaggc aaaaatggaa aaacttagtg caatcggata caaagaacct ttctattcgc
    20341 ttgaagatgg tgtgtcagat tatgtatgta attatctgtt gcccgcaaaa tattattaat
    20401 tcatagttta tcaacaatga ttaacagcaa atcaggaaaa cctattttag cgccctgatg
    20461 ataaaattca ctatgcgtgt tctgaacctg aaagtggttc ggtacttttt ctcagcagca
    20521 tctgcaacaa ttgtagatgt aggaatttat tttttggcat ttaattattt gttcgaaaag
    20581 aaggatattg tcttttcatt ttatactttt tctgcaccaa cagtttctct aatgttgtct
    20641 tattcatgcg gattgttaac gaactttttt cttacaaaaa ctttagtctt taaagaatca
    20701 gatttaaaag gacatcatca gtttttgcgt tttgtattgg tagctattgg tgtcttgttt
    20761 ttgaattatt tgctgatgaa attccttatt cgtcagatgg attggtatcc tacaatagca
    20821 cgtgcttttt ctgcagtgag tataggactg ataagttttg ttgttcacaa aagcttttcg
    20881 ttcagagtaa gtgcaacaga agaagtagaa gattaatctg ctactgtccg aatatagaga
    20941 gacgtttcac tactttcatt gttccattaa attggatttt gaaaagataa agcccatcca
    21001 caggcattgg attgatattt accaaacact tacctttttc catataacaa tctgatgtga
    21061 aaattaattt accggttaag tcagttattg aaatcgagat atttccagaa taattacctg
    21121 ctttaatatt gatatcattt ccactgagtg gattagggta aatgtttatt tcattatacc
    21181 atgtaatgat attaatagca ttgatagtgt cgcagtccca aagccctgtg tatccaccta
    21241 cacatattcc acaagaatcc aaataggcag tgccacccca atcaccatga caatcttgtg
    21301 tgcatggttg aagacctgta tttccaccaa cgcatgcgtt gcatgaatca atgaaagcag
    21361 taccacccca atcaccgtga caatcttgtg tgcatggttg caaacctgtg tttccaccaa
    21421 cgcaagtgct gcatgaatca atgaaagcag taccacccca atcaccatga caatctt
//
LOCUS       DAOOOI010000039        21165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_483_length_21165_cov_33.273141, whole genome shotgun sequence.
ACCESSION   DAOOOI010000039 DAOOOI010000000
VERSION     DAOOOI010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 21165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21165
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_483_length_21165_cov_33.273141"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3603)
                     /gene="porU"
                     /locus_tag="PKX59_06440"
     CDS             complement(<1..3603)
                     /gene="porU"
                     /locus_tag="PKX59_06440"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOZ90541.1"
                     /translation="MKRSFALLFLIGLTCVAVAQQDESLKRTYSVDWRSNVVLNGENR
                     LNFTNCFYKPDSKMPFFSTGLPLFDYTKVAEVSIENPVYEPLNINISDEVASEIVPET
                     KLSYFKGKPSLYVEFVPVRKNSVTGQTEKLVAFNLKTTAGQQSQKIGATSTQVYAANS
                     VLASGDWYKISVTEDGVYKITYSFLKDQLKIDLSSSSPQSFRLFGNGGGILPLLNSQF
                     RYDDLQENAVFVNDGGTAGKWDDQDYILFYGQSPVQWLFNSSQNRYYHQANYYSDSTF
                     YFITFNGGTGSPKRIQQSASLSVTPDFVVNSFDDYQYHELDKTNFVKSGREFYGESFD
                     INLNQTFSFTFPNILQQTVTIKTVFAGHSPGVTSSASARYQNITLFSGTYTTGSQYTD
                     DYAMERTTTSTFLPSAGDVVNIDFAFTPANTECIGFLNYIELLAKRALTFAGNGATDQ
                     MFFRSMEASGPGKTAQFNIASAPSSMTVWNVTDRINIRQQLHNNGSFITQVDSTQQFL
                     AFGGNNFLTPGVIGTVANQNLHSLPQADLIIVTHPLFEDQANRIADMHRTLDNMSVNV
                     VRTDLIYNEYSSGAQDIAGIRNFVKMFYDRGIGNGTEPKYLLLFGDGSYDNKYRRANN
                     TNFIPTFESENSYSFLSSFVADDFYGLMDDNEGLCSSNEKIDIGVGRIIAQNISEAKT
                     VTDKTIQYASLPQSSGCPGCDGSGGTLGDWRNVVTFIADDEDGPIHVPPAESFANYLN
                     TYHKTYNVDKIYLDAYKQISTPAGQRYPDVNDAIDKRVNKGSLIINYVGHGGETGWAE
                     ERVLTNDDINSWTNINKLPVFITATCEFSRWDDPARTSSGEKILLSPTGGGVALFSTT
                     RLVFASSNEVLNRALIKFMFDDPNYRLGDIMVASKTDPGNLNLNTRNFSLLGDPALRL
                     HYPKFNIEATQINAQPIIALNDTLSSLEKVTIAGRIVKGGQLQSDFNGFLYPTVYDKA
                     SSITMLRNDASSPNITFKLQKNILYKGKATVKNGEWSFTFIVPKDISYQFGLGKLSFY
                     AENGVEDGAGYFDSIVVGGSSANIISETDGPGIKLYLNDERFAFGGTTNEKPTIVCLL
                     SDSTGINTVGNGVGHDITAIIDGKVDPVYVLNDYYEADLDSYSSGRVAYPLSKLPEGR
                     HTLKVKAWDILNNSSEAYTEFIVASDASLALKHVLNYPNPFTTHTQFSFEYNKPC"
     gene            3810..4808
                     /locus_tag="PKX59_06445"
     CDS             3810..4808
                     /locus_tag="PKX59_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOZ90542.1"
                     /translation="MIKRIVLTMIISLELAICFGQDPQFSQFYANPIYLNPAFAGSVR
                     CPRLALNYRNQWPGLNKTFITLSASYDQHVEALSGGLGLIIMNDKAGNGTLNTTNFSG
                     IYSYNLNISRNFSIRAGLQATYVQEKLDWDKLTFGDMIDPRYGYVFETQEVRPAEKRG
                     FADFSAGVIGYSSKVYGGFAVHHITEPDQAFIVEGSSPLPRKYTAHVGALLPLKGAST
                     YRGKLEGTFISPNFLYQQQQGFNQLNFGLYVIKSPIVGGLWYRGYTGKKFLSSDSFIA
                     LIGLQKDMFKFGYSYDVTVSKLNNNVTAGSHEISLGLQFECRPKKKRFKAISCPTF"
     gene            4850..6298
                     /locus_tag="PKX59_06450"
     CDS             4850..6298
                     /locus_tag="PKX59_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOZ90543.1"
                     /translation="MNLRKNAKTLAFVALVVMATNACKQKEKSSTTGWKYNDEKNGGF
                     EVTPYTEQETGPGLILVEGGTFAMGRTEQEIPYDWDNIPRRVTVSSFYMDETEIANVH
                     YLEYLYWLDRVYGTDYPEVYKKALPDTLVWRSKLGYNEPMVELYLRHPAYQRYPVVGV
                     NWLQANDFASWRTDRVNEQILVREGILRVNPAQVNEDNFNTDAYLAGQYEGLVKSKLQ
                     DLNPNRDERNVRMEDGILLPRYELPTEAEWEYAALGLIGNTVYERVTDRKIYPWNGHV
                     VRNSSEKYKGEMLANFKRGRGDMMGTAGALNDNADRPAPVFSYLPNDYGLYNMAGNVS
                     EWVKDVYRPLSPEDMDDFNPYRGNVFVTPLLDEEGQYAEKDSLGRMVWREVDEKENEA
                     RRNYTKADYINDLDGDEPDNIEYKMGGTTMIDDNAHVIKGGSWKDRAYWMSPGTRRFL
                     DQQQSTDWIGFRCSMVRVGSPVGFNQKASKKG"
     gene            6373..7674
                     /gene="murF"
                     /locus_tag="PKX59_06455"
     CDS             6373..7674
                     /gene="murF"
                     /locus_tag="PKX59_06455"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010181611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOZ90544.1"
                     /translation="MIAHLHSLFLNSTGVCTDTRKLLKGNIYFALKGPSFDGNKFALQ
                     AIQQGAIAAIVDDSSLKGENIYLVENVLTTLQQLATYHRQVLKIPVIGLTGSNGKTTT
                     KELISRVLGTSYNVLFTEGNLNNHIGVPLTLLRLKSEHQIAVIEMGANHQGEIRELSM
                     IALPNFGLITNIGKAHLEGFGGIEGVIKGKTELYRHLMENDGVIFCRKNNELLTNNLN
                     HYQKVIFYSTQVDNCICGEISTPASVVKFNWFENQNRIDEIITHLTGDYNLENLVAAV
                     CIGRYFNITPDKIKTAIEGYIPENQRSQIEISGSNTIIIDAYNANPSSMEAALKNLSE
                     NYDGNKVIILGEMLELGQDANTEHQHIVDLLSSMEKDLLILVGENFLHTTLNDHALRF
                     KTSEDACNYLHNHAINESTILVKGSRGSKMEKVLDGIRNHN"
     gene            7747..8334
                     /locus_tag="PKX59_06460"
     CDS             7747..8334
                     /locus_tag="PKX59_06460"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013072519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOZ90545.1"
                     /translation="MKNTNTEIFNDDIKSCLEILRQGGLIVYPTDTIWGIGCDATNDA
                     AVEKIFKLKNRSESKSLITLVSDEAMLNRCVQNVPAVAWDIIEYSEKPVTIIYEKISR
                     ISEKAIAEDGTAAIRLVKDSFCKMLIHKFGKPIISTSANMSDRPSPAVFNDIEHSILS
                     GVDYVVKYRQQENTKHTASSIIRILNNGEVKVIRK"
     gene            8306..9748
                     /locus_tag="PKX59_06465"
     CDS             8306..9748
                     /locus_tag="PKX59_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOZ90546.1"
                     /translation="MAKLKLSENNLQTEMLEHSLFRQIASLCEKHNLKAFVIGGFVRD
                     SFLKRPSKDIDIVVNGDGIAVAKELAAVLEGEFAFFKNFGTAMVKSEGFEIEFVGARK
                     ESYSQHSRKPKVSPGTLEDDQLRRDFTINTLAFSLNSDDYGKLIDPFNGKQDLNDKII
                     RTPADPVTTYSDDPLRMMRAIRFATQLNFSIEEKSLAAIQSEKSRISIVSKERITDEL
                     NKIIASPLPSTGFKLLMDTGLLELIFPQFYNLKGVDYFEGKGHKDNFYHTLEVLDNIA
                     KVTDNLWLRWAAILHDIAKPATKRFEPGHGWTFHGHEDKGSRMVPPIFRELKLPLNEK
                     MKYVQKLVLLHLRPIVLAQELVTDSAVRRLLFDAGDDIDDLMLLCQADVTTKNEYKKA
                     KYRNNFELVIQKLKEVEEKDRIRNWQPPVTGQLIMDTFGLPPGPNVGIIKDFVREAIL
                     DGDCPNEQQAAFELMLKKGLELGLTPQKKS"
     gene            9826..11637
                     /gene="typA"
                     /locus_tag="PKX59_06470"
     CDS             9826..11637
                     /gene="typA"
                     /locus_tag="PKX59_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOZ90547.1"
                     /translation="MQNIRNVAIIAHVDHGKTTLVDKILHQTQVFRQNQQTGELILDN
                     NDLERERGITIVAKNVSVQYKDVKINIIDTPGHADFGGEVERVLTMADGVILLVDAFE
                     GPMPQTRFVLQKALALGLKPIVVINKVDKENCRPDEVHESVFDLFFHLEATEEQLDFP
                     TLYGSSKHGWMSTDWRKETSDITALLDAIIEKTPPAKSLEGTPQMLITSLDYSNYVGR
                     IAIGRIVRGTLKENMPVTLMKRDGSAKKSRIKELFVFEGLGRKKVDSVSSGDIAAIVG
                     VDDFEIGDTIADFENPEALKSIAVDEPTMSMLFTINNSPLFGKEGKFVTSRHLRDRLY
                     KETEKNLAMRVEETNSPDAYLVYGRGILHISILVETMRREGYELQLGQPQVLYKEIDG
                     IRCEPVEHLVVDTPSEVAGKVIELATQRKGELTSMLPKGDLQRLEFNIPARGLIGLRN
                     AVLTATAGEAIMAHRFNSYEPAKGTIPGRNMGVLLAKEAGSSTAYSIDKLQERGAFFI
                     DPGETVYGGQVIGEHIRPGDLVVNVCEGKKLTNIRASGSDDNARITPKIKFSLEEAME
                     YIEKDEYLEVTPQNIRMRKIYLDENERKRMSKKAEMA"
     gene            11702..12091
                     /locus_tag="PKX59_06475"
     CDS             11702..12091
                     /locus_tag="PKX59_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HOZ90548.1"
                     /translation="MKAVQKKQKVKMEFPMRSSPKILYGFLSTPSGLSEWFADDVTIR
                     EGVYTFHWDGDEQQAKMTHKRENQMVRFKWIDDSDDAFIEFEITTDELTSDVSLMVSD
                     FAVEDDIEETQRLWDTQIHNLRHLIGS"
     gene            complement(12144..12752)
                     /locus_tag="PKX59_06480"
     CDS             complement(12144..12752)
                     /locus_tag="PKX59_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOZ90549.1"
                     /translation="MLNLVMHAWEIQSVSIDSIHKEYDAAIMMGGAIRYVNGQMQRPV
                     FGSGADRYVQSLELFYRNKAKYIIISSGSGSLVYTDVKEADLLRNQMIRIGVPADKVI
                     AENQSRNTYENAKNTAEIIRKSDFRPPFLLVTSAFHMRRSMLCFQKQGIDVVPFAVDQ
                     HSGQIMFTPDKLFLPSPEVLLDWDLLFHEWFGIAVYKVMGYL"
     gene            complement(12985..13776)
                     /locus_tag="PKX59_06485"
     CDS             complement(12985..13776)
                     /locus_tag="PKX59_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOZ90550.1"
                     /translation="MKNDFKNEKWSLVKPPFRFTNNLKIEVSNFGRVRTSTKIAENRV
                     LKGTLINGYPIVRFKLFKKRADDAQKQLDQKRRKIEQLTGKLRDVKSELKSYKKNSPA
                     YNSNKKKVDNVAAELSTLKEVYRNVFRKDELERTINYAPLVHRLVADSFVKQPSKKHD
                     IVAHLDYNKSNNHFSNLKWMTQEDNIEHQKKSPVVIKEKKKRIGKRPENASGYKLNEK
                     KVAEIKKRIQKGETLSKISDRFNVSETQLLRIKRGINWGDVKVPH"
     gene            complement(13837..14202)
                     /locus_tag="PKX59_06490"
     CDS             complement(13837..14202)
                     /locus_tag="PKX59_06490"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HOZ90551.1"
                     /translation="MLQVTGILKQKFAEQVVTDKFRKREFTLTIDAKSAYPQIVIFQL
                     ANDKCRLIESFQPGDELSVSFNIKGREWKSPSGEIKYFTSLDAWKIERTSAGESPMES
                     PMPSMSDLPQEDYKDDLPF"
     gene            complement(14234..15358)
                     /locus_tag="PKX59_06495"
     CDS             complement(14234..15358)
                     /locus_tag="PKX59_06495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ90552.1"
                     /translation="MKNSGKHLHIISFDIPYPANYGGVIDVFYKLRALVSAGIKIHLH
                     AFQYGRQSSKILEELCESVHYYQRDVSKSQLFLKEPYIVVSRSSEQLIKNLLSDEHPI
                     LFEGLHSTHYLSDERLKKRKKIVRTHNIEHEYYKNLAKAESNIFKRYYFYNESSKLEI
                     YENILSNADVIATISPNDTQYFKSKYGNAHYIPAFHSFTKVNSRIGKGAYAFYHGNLA
                     IGENDMAALYLVQEVFKNSPVTLLIAGSKPGVNLRDAVQRKNNITLLSDLNTKQINDL
                     VLDAHINVLPTFQPTGIKLKLLSALFSGRFALVNTPMVQNTGLEQLCTVADTPEAMRE
                     ALAAMIKQEFTQIDISLREEILMENFNNKANALKLKNLIF"
     gene            complement(15382..16515)
                     /locus_tag="PKX59_06500"
     CDS             complement(15382..16515)
                     /locus_tag="PKX59_06500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ90553.1"
                     /translation="MAQKKVIISVINDIATDQRVRRTASVFSELNYQILIVGRKLPLS
                     LPVYDMTGLSVKRFNLPINKGPFFYALFQIRLFFFLIFKKADVLYANDLDTLLPNYVV
                     SRLKGCNLIYDSHEYFTGVPELQNNHIARKVWTRVEAFIFPKLKNVITVNNSIANLYH
                     QQYGNDVKVVRNVPDRTDSVNFDVAKWKRSVGFPDYKKVFIMQGAGINVDRGAEEAIE
                     AMKDVDAMLLIIGGGDVFHQLQELAIKHNVKHKVIIHPKISREELITVTSACFAGLTL
                     DKDTNINYKFSLPNKLFDYIHAGIPVIASDLPEVTAIVNGYKTGIVLPQYSPQAIAEA
                     MNRLVIEKELYVSYKENCSIASKDLCWQKEKKVLIDLILSSVN"
     gene            complement(16562..17425)
                     /locus_tag="PKX59_06505"
     CDS             complement(16562..17425)
                     /locus_tag="PKX59_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOZ90554.1"
                     /translation="MRQLRIQKSITNRESQSLEKYLQEISREGMITAEEEANLARRIR
                     EGDEEALSKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAASRFDETRGFKF
                     ISYAVWWIRQSILQAIAEQARIVRLPLNQVGSLRKINKVFSKLEQEFEREPSAEEIAE
                     MMEVPIEKISDTLKSPGKHVSMDAPFAGNEEATLLDVMQNDDAPDADFGLMNESLRKE
                     IDRSLKTLTERERDVVKLFYGIGVSHSFSLDEIGAKFGLTRERVRQIKEKAIRRLRSG
                     SRNKLLKDYLA"
     regulatory      17584..17684
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            17731..20184
                     /locus_tag="PKX59_06510"
     CDS             17731..20184
                     /locus_tag="PKX59_06510"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ90555.1"
                     /translation="MIKKFSLLLLALCLSLYSNAQLDLSGVVSTNDGKKVDHAVVTLK
                     ESLIRHFSDEKGKFTFFNLKPGKYTLQVNQLGYHEYSSEINLASNQHIEVILFKKAFV
                     TDEVIIQSLKMKNKIPIAQNSLNKNEIEEMNSGKDVPEIMDQLTSVVVSSDAGAGVGY
                     TGMRVRGSDATRINVTINGIPINDAESQSMYWVDMPDLLSSTNQIQLQRGVGTSVNGA
                     AAFGASINILTDNLSEKPFAETNLSYGAFNTKKVNLIAGTGLLNNQFAFETRLSSIQS
                     DGYVDRASSNLKSFYFSGGYFGKKSMLKAIIFSGIETTYQSWYGVPESRYKNNMQGMM
                     DYISRNGLDSEDATNLLESGRKYNYYLYPGQTDNYQQDNYQLHFSHQINNLFTLSVAA
                     HLTRGKGYYEELKKNAKFSDYGLPPIQIQDTIITKTNLVRRKWLDNYFYGLIYSLTYE
                     NKNLEIKTGGGINQYDGDHYGKIIKGDIIPLASENYTYYQNNGFKSNFNHWIKGTLHL
                     GEHFIAYADIQYRYVHYRFTGFDDFATTTNQVAEFHFFNPKAGITFLRNENESAWLSI
                     AVGNKEPSRDDFTESTAKSRPKSENLIDFEAGYSIAIKKLKLSLNAFYMQYKDQLILT
                     GKINDVGNYTRQNVKNSFRRGLEAEFNYDVGKFLAVSGNVALSENKILNFKEYIDQYD
                     ADFNYTGQQKIIYKKTDIAFSPSIITAVGIEIRPLKMLSIKLTEKYVGKQYLDNTSSE
                     EKSLKAFAYTNLSIHYTFKIFKTIETEAGVAVYNLFNEMYSSNGYTYGYLYDGMRVNE
                     NFYYPQAGTHTMLTAKFRF"
     gene            complement(20174..20968)
                     /gene="dapF"
                     /locus_tag="PKX59_06515"
     CDS             complement(20174..20968)
                     /gene="dapF"
                     /locus_tag="PKX59_06515"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOZ90556.1"
                     /translation="MHLKFSKYQGAGNDFVMIDNRSGKYSSLKVQQREMLCDRHFGIG
                     GDGLILIQHSLKSDFEMVYFNADGRLGSMCGNGARCAVLFSKQLGMIGRETLFMAYDG
                     LHSAKVSAAKALKSAIVSVSMSDVSEIKKHNGNFILNTGSPHYVKFVDDTDKIDVVKE
                     GKKIRNSNTFKAKGINVNFVSFKSKASHIRTYERGVEDETLACGTGITASAIAAHYSG
                     LTKKLSVNVIARGGSLNVTFQVEANKYKNVMLTGSAMNVFDGEIKI"
BASE COUNT         6877 a         3860 c         3820 g         6608 t
ORIGIN      
        1 acaaggcttg ttgtattcaa atgaaaactg cgtatgcgtt gtaaacgggt tgggataatt
       61 taaaacatgc tttagtgcta atgatgcatc agaagccaca ataaactctg tataagcttc
      121 acttgagttg ttgagaatat cccatgcctt tactttaagc gtatgccttc cttctggaag
      181 ttttgatagc ggataagcaa ctctcccact agaataactg tctaagtccg cttcataata
      241 atcattgagt acgtaaacgg gatcaacttt tccatcaatt attgctgtga tgtcatgccc
      301 aacaccatta ccaactgtat tgatacctgt tgagtcagag agcaaacata caattgtagg
      361 tttttcatta gtagtgccgc caaaagcaaa gcgctcatcg ttaaggtata gttttatacc
      421 gggtccatct gtctcagaaa taatatttgc agacgaacca cccacaacta tgctatcaaa
      481 atatcctgca ccatcttcta ctccattttc agcataaaag cttagcttgc ctaacccaaa
      541 ctgataagaa atatctttgg gtacaataaa tgtaaacgac cattcaccat tttttactgt
      601 agcttttcct ttatataaaa tatttttctg aagcttaaaa gttatgttag gacttgatgc
      661 atcattgcgc aacatggtta tgcttgatgc tttatcatat acggttggat ataaaaaccc
      721 attaaaatca gactgaagtt gtccaccttt aactattctt cctgcaatgg ttactttctc
      781 taatgagctt aaagtatcgt ttagcgctat aattggttga gcgttaattt gtgtagcctc
      841 aatattgaat ttaggataat gtaatcttaa tgccggatca ccgagtaaac taaaattacg
      901 tgtattcaga ttcaaatttc ccggatcggt tttactggca accatgatat cgcctaaacg
      961 ataatttgga tcatcaaaca taaactttat caaggctctg ttaagaactt cgttgctgct
     1021 tgcaaatacc agtcgtgtag ttgaaaacaa tgcaacacct ccaccggttg ggctcaataa
     1081 tattttttcg ccagatgatg ttcttgcagg atcgtcccat ctgctaaact cacaggttgc
     1141 tgtaataaat accggcagtt tattaatgtt tgtccatgag ttaatatcat cattggtaag
     1201 aactcgctct tctgcccatc ctgtttcgcc accatgacct acataattaa taatcaacga
     1261 acctttattg acacgtttat caatagcgtc attaacatca ggataacgct gacctgcagg
     1321 ggttgatatt tgtttgtagg catctaaata aattttatca acgttatatg ttttatgata
     1381 tgtatttaaa taattggcaa acgattctgc aggtggtacg tgtataggtc catcctcatc
     1441 atctgcaata aacgttacca cattgcgcca gtcacctaat gttccaccac taccatcaca
     1501 acccggacaa ccactactct gtggcaatga tgcatattga atggttttat ctgtaaccgt
     1561 tttagcttcg ctgatgtttt gtgcaataat tcgacctaca ccaatatcaa ttttttcatt
     1621 gcttgaacac aaaccttcat tgtcatccat caaaccataa aaatcgtcag caacgaatga
     1681 gcttaaaaat gaatatgagt tttcagactc aaaggttgga ataaaattgg tgttattggc
     1741 tctgcggtat ttgttgtcat aagaaccatc accaaataaa agtaaatatt ttggttctgt
     1801 tccattgcct atccctctgt cataaaacat ttttacaaaa ttgcgtatgc ctgcaatatc
     1861 ctgtgctcct gaagagtatt cgttgtatat taaatctgtt ctcacaacat tcacagacat
     1921 gttgtccaat gttctgtgca tatctgctat gcggtttgcc tgatcttcaa ataatggatg
     1981 tgttacaatg attaaatcgg cttgtggcaa tgaatgtaaa ttttgatttg ccactgtacc
     2041 aattactccg ggcgttaaaa aattattccc accaaaagct aaaaattgct gtgtactgtc
     2101 cacctgtgta ataaacgaac cattattatg taattgttgt ctgatattta ttctgtcagt
     2161 gacattccaa accgtcatag atgatggtgc cgatgcaata ttaaactgtg cagttttacc
     2221 ggggccggaa gcttccatac ttcggaaaaa catctgatcg gtagcaccat tacctgcaaa
     2281 tgttagtgct cgctttgcta ataattctat atagttcaaa aagccaatac actctgtatt
     2341 tgcaggagta aaagcaaaat caatattcac tacatcaccg gcagacggca agaacgttga
     2401 agttgttgta cgttccattg cataatcatc tgtatattgt gaaccggttg tgtatgtacc
     2461 cgaaaataat gttatatttt gatagcgtgc cgaagccgaa cttgtaacac caggtgaatg
     2521 acctgcaaat actgttttta tagtcacagt ttgttgcagt atatttggaa atgtgaatga
     2581 aaaagtctga ttaaggttta tgtcgaatga ctcgccataa aattcacgtc cagatttgac
     2641 aaaatttgtt ttatccaatt catgatattg gtaatcatca aaactgttta caacaaagtc
     2701 aggtgtcact gataatgatg ccgattgctg aattctttta ggacttccgg ttcctccatt
     2761 aaacgtaata aaataaaatg tggaatcgga ataataattt gcctgatggt aatatctgtt
     2821 ctgagaggaa ttaaaaagcc attgcacggg tgactgtcca taaaacaaaa tataatcctg
     2881 atcatcccat tttcctgctg tacctccatc atttacaaac acagcatttt cctgcaagtc
     2941 atcatatcta aactgagagt ttaatagagg taaaatgcct ccaccattgc caaagagcct
     3001 gaatgactga ggtgaggaag atgataaatc aatttttaac tggtctttca gaaaactgta
     3061 tgttatttta taaactccat cttctgtaac tgaaatttta taccaatcac ccgaagcaag
     3121 aactgaatta gcagcgtaaa cctgagttga agtggcacca attttttgac tctgttgccc
     3181 ggctgtcgtt ttaagattaa atgcaactaa tttttctgtt tgccccgtaa ctgaattttt
     3241 tcttaccggc acaaattcta catacaaaga tggttttccc ttaaagtaag aaagttttgt
     3301 ttccggaact atttccgatg caacttcatc agaaatatta atatttaatg gttcataaac
     3361 aggattttca atgctaactt cagccacttt agtataatca aatagtggaa gtccggtaga
     3421 gaaaaatggc atcttgctgt ccggcttata aaaacagttg gtaaaattaa gcctgttttc
     3481 gccatttagg acaacattac tgcgccaatc aacggaataa gtgcgtttta aagattcatc
     3541 ttgttgcgca acagcaacac aagtcaatcc tattaaaaaa agtagtgcaa aagacctttt
     3601 catgttttgg taattgggtt caaaaatatg cttctcgaac aatacaacat gtatgaaaca
     3661 tgcaatattg aattttattt tgtttgatat taaaataatt aatgcctaac tttgttccga
     3721 ttaaattttt aaccaccaat atattcgtgc agaattgtaa ctattttagt actatacgta
     3781 taaaattaag tttgaaaagt aaatgaacta tgattaagcg tattgtactt accatgatta
     3841 tcagcctgga gttagctatt tgttttggcc aggacccgca gttttcgcag ttttatgcca
     3901 atccgattta tctgaatcct gcctttgccg gttcggttcg ttgtccacga ctggcattga
     3961 actatcgtaa tcagtggcct ggtttaaata aaaccttcat cactttgagc gcctcttatg
     4021 accaacatgt tgaagcactc tccggtggat taggcttaat cattatgaat gataaagcag
     4081 gtaatggaac actgaataca accaacttct ccggcatcta ttcttacaac ttaaacattt
     4141 cacgaaattt ctctattcgt gcagggctgc aagctaccta tgttcaggaa aaattagact
     4201 gggataaact gacctttggc gatatgattg atccccgcta tggttatgtg ttcgaaacgc
     4261 aggaagtacg tcctgccgaa aagcgtggtt ttgctgattt ttcagcaggc gttataggtt
     4321 acagttcaaa agtatatggc ggttttgctg ttcatcatat tacagaacct gatcaggcat
     4381 ttattgttga aggctcaagt ccgttaccaa gaaaatatac tgcacacgta ggggcattat
     4441 taccattaaa aggcgcttca acttacagag ggaaattaga aggtacattt atctcaccaa
     4501 actttttgta tcagcaacag caaggattta accaactcaa ctttgggctt tatgttataa
     4561 agtcgccaat tgttggtggt ttgtggtaca ggggctatac aggtaaaaaa ttcctgtcaa
     4621 gcgacagctt cattgccctc atcgggttac aaaaagacat gtttaagttc ggttattcct
     4681 atgatgttac cgtttctaaa ctaaataata atgttactgc cggatctcat gaaatttctt
     4741 taggtttgca gtttgaatgc cgtccaaaga aaaaacggtt caaggcaata agttgcccca
     4801 cattttagtt taatgacata aaccatataa agtttccaaa tacgtaaaaa tgaatctccg
     4861 taaaaatgca aaaacccttg ccttcgttgc tttagtagtg atggcaacaa atgcttgcaa
     4921 gcaaaaagag aaatcttcaa ccacaggatg gaagtacaat gacgaaaaga atggtggatt
     4981 tgaagttact ccatataccg aacaggaaac aggtccggga ttaatccttg tagaaggtgg
     5041 aacatttgca atgggccgta cagagcaaga aattccttat gactgggata acattcctcg
     5101 cagagttact gtttcatctt tttacatgga cgaaacagaa attgccaatg tacattattt
     5161 agaatatctc tattggttag accgtgttta tggaactgac tatcctgaag tgtataaaaa
     5221 agcgcttcct gacacattag tttggagaag taaattaggt tataacgaac caatggttga
     5281 gttatattta cgtcaccctg cctatcagcg ctatcctgtt gttggtgtaa attggttaca
     5341 agccaatgat tttgcaagtt ggcgtaccga tcgtgtaaat gagcagatat tagtgcgcga
     5401 aggaatttta cgtgttaacc ctgcacaggt aaacgaagat aactttaata ctgatgcata
     5461 tcttgccgga cagtacgaag gtttggtgaa aagcaaactt caggatttaa accccaatcg
     5521 tgatgaacgt aatgtgcgta tggaagatgg aattttatta ccacgctacg aacttccaac
     5581 agaagcagaa tgggaatatg cagccttagg cttaattggc aatacggttt atgagcgtgt
     5641 tactgacaga aaaatatacc cttggaatgg tcacgtagta agaaattctt cagaaaaata
     5701 taaaggtgaa atgcttgcca acttcaagag aggtcgtggt gatatgatgg gaacagcagg
     5761 tgcattaaat gacaatgcag accgtccggc tccagtattt tcatatttac caaatgatta
     5821 tggtttatat aatatggcag gtaacgtttc ggaatgggta aaagatgttt atcgcccctt
     5881 atcacctgaa gacatggatg actttaatcc ttacagagga aacgtatttg taacaccatt
     5941 acttgacgaa gaaggacaat atgcagaaaa ggatagtctt ggtcgcatgg tatggcgcga
     6001 ggtagatgaa aaagaaaatg aagctcgcag aaattatact aaagccgatt acataaacga
     6061 tcttgatggt gatgaacctg ataacatcga atataaaatg ggtggtacta cgatgattga
     6121 tgataatgca cacgttatta aaggtggttc ctggaaagac cgtgcatatt ggatgtcacc
     6181 cggtacaaga agatttttag accaacaaca atcaactgac tggattggat tccgttgttc
     6241 aatggtacgt gtaggtagcc ctgttggttt taatcaaaaa gcgtctaaga aaggataata
     6301 tccttcaaca tttaaataaa atacataagc cccttttttt ggggctttct ttttgaaagt
     6361 tattgaatat aaatgatagc acatttacat agtttattcc tgaactcaac cggagtttgt
     6421 acagatacaa ggaaactgct caaaggaaat atttattttg ctttaaaggg accttctttt
     6481 gatggaaata aattcgcact acaagcaata caacaaggtg ccatagcagc aatagtggat
     6541 gattcctcac taaagggtga aaacatttat ttagtagaga atgttctaac gactttgcaa
     6601 cagttagcta cctatcacag gcaagtttta aaaataccgg ttattggatt aacaggtagc
     6661 aatggaaaaa ctactacaaa agaattaatc agcagggtac tcggcacgtc atataacgtt
     6721 ttatttactg agggaaatct gaataatcac atcggtgttc cacttacctt gttaagatta
     6781 aaaagcgaac atcagattgc agttattgaa atgggagcca atcatcaggg agaaattcgt
     6841 gaactgtcaa tgattgctct gcccaacttc gggctcatta caaatattgg caaagcacat
     6901 ctcgaaggtt ttggaggtat tgaaggtgta attaaaggaa aaacagaatt gtacagacac
     6961 ctgatggaaa atgatggagt aattttctgc cgtaaaaaca atgaattgct aacaaataat
     7021 ctcaatcatt atcaaaaggt aattttctac agtacgcaag tggacaattg tatctgtggt
     7081 gaaatttcta ctcctgcatc agttgttaaa tttaattggt tcgaaaatca aaataggatt
     7141 gatgaaataa taacgcacct taccggtgac tataatcttg aaaatcttgt agcggcagtt
     7201 tgcattggta gatattttaa tattacacct gataaaatta agacagcaat tgaaggttac
     7261 atccccgaaa atcagcgttc gcaaatagaa atctcaggaa gtaatacaat tattattgat
     7321 gcatacaatg ccaatccttc aagtatggaa gctgcattaa aaaatctttc tgaaaattat
     7381 gatggcaata aagttatcat tcttggagaa atgcttgaac ttgggcagga tgcaaataca
     7441 gagcaccaac acattgttga cttattgagt agtatggaaa aagacttact tattttagta
     7501 ggtgaaaatt ttctgcatac aacactcaat gatcatgcat tgcgctttaa aacctccgaa
     7561 gatgcttgca actatttaca taatcatgct attaatgaaa gcactatttt agttaaaggc
     7621 agtcgcggtt ctaaaatgga aaaagtgctt gatggcatcc gcaatcacaa ctgatttttt
     7681 taaaaaatca ttagctcaag aattaaaaag taaaaattcg tttcaatctg ttttctttgc
     7741 attgtgatga aaaatacaaa taccgaaatt tttaatgatg acattaaatc ttgtcttgaa
     7801 attttaagac aaggaggact tattgtttat cctactgaca caatttgggg aattggatgt
     7861 gatgctacaa acgatgctgc agtagaaaaa atttttaaac ttaaaaaccg gagcgagtca
     7921 aaaagtttaa tcacattagt gagcgatgag gcaatgctga atcgttgtgt gcaaaatgtt
     7981 ccggctgttg catgggacat cattgaatat tccgaaaagc ctgtaactat aatttatgaa
     8041 aagatcagtc gtatctctga aaaagcaatt gcagaagacg gcaccgcagc aatcagatta
     8101 gtgaaagatt cattttgcaa aatgctgatt cataaatttg ggaaaccaat catatctact
     8161 tctgcaaaca tgagcgacag gccctcccct gctgttttta atgacattga acattccata
     8221 ctttcaggag ttgactatgt tgtaaagtac agacaacaag aaaacacaaa acatacagca
     8281 tcatccatca tcaggatttt gaacaatggc gaagttaaag ttatcagaaa ataatctgca
     8341 aacagaaatg cttgagcata gtttgttccg gcagattgct tcactttgcg aaaagcataa
     8401 tttaaaagca tttgtcattg gaggatttgt gcgtgatagt tttttaaaaa gaccttcaaa
     8461 agatattgat attgtagtta acggtgacgg cattgccgta gctaaagaac ttgctgcagt
     8521 tcttgaagga gaatttgcat tctttaaaaa ctttggtact gcaatggtaa aatccgaagg
     8581 atttgaaatt gaatttgtag gtgcaagaaa agagtcgtat agtcaacatt cgcgaaagcc
     8641 gaaggtttca cctggcacat tagaagacga tcagcttaga cgcgatttta ctatcaacac
     8701 acttgctttt agtcttaaca gtgatgatta tggaaagctg attgacccat ttaacggcaa
     8761 gcaggatctg aacgataaaa ttattcgcac acctgccgac cctgtaacaa cttacagtga
     8821 tgatccactg cgcatgatgc gtgcaatacg ctttgcaaca caacttaatt tcagcattga
     8881 agaaaaatcg ctggctgcaa ttcaaagtga aaagagtaga atcagcatag tttccaaaga
     8941 acgaataacc gatgaactga ataaaattat tgcttcgcca ttgccatcaa caggattcaa
     9001 attgttgatg gatacaggat tgttggagtt aatatttccg cagttttaca acctgaaagg
     9061 agttgattat tttgaaggca aaggtcataa agataacttc tatcacacct tggaagtatt
     9121 ggacaacatt gcaaaagtca ctgataattt atggttacga tgggctgcta ttctgcatga
     9181 cattgcaaaa cctgcaacaa aacgttttga acccgggcat ggatggactt ttcatgggca
     9241 tgaagacaaa ggttcacgta tggttcctcc tatcttcaga gagttaaaat tacctctcaa
     9301 tgaaaagatg aagtatgtac aaaaattggt tttgctccat ttaagaccca ttgttttggc
     9361 acaggaactt gttaccgatt cagcagtacg cagactgtta tttgatgccg gtgatgatat
     9421 tgacgacctg atgttgttgt gccaggctga tgtgacaact aaaaatgaat ataagaaagc
     9481 caaataccgt aacaactttg agttggtcat tcaaaaactt aaagaagttg aagagaaaga
     9541 cagaatcaga aactggcaac cacctgttac cggacaattg attatggata catttggttt
     9601 gccgcctgga cctaatgttg gtattataaa agattttgtt cgcgaagcaa ttttagatgg
     9661 cgattgtcct aatgaacaac aggcagcatt tgagttaatg cttaaaaaag gcctcgaact
     9721 tgggctaaca ccacaaaaaa agagctgatt ttttctactt ttattattga caatcaatat
     9781 ttgggcggta aaaagtctta tctttgcacc ccaaataaaa ttttcatgca aaatatcaga
     9841 aatgtggcca ttattgccca cgttgaccat ggtaaaacaa ccttagttga taaaatactt
     9901 catcagacac aggttttcag acaaaatcag caaacaggag aattgattct cgacaataat
     9961 gatctggaac gtgagagagg aattaccatt gttgcaaaaa atgtatctgt tcagtacaaa
    10021 gacgtaaaaa ttaatattat tgacacaccg ggtcacgctg actttggagg agaagtagaa
    10081 cgtgttctta caatggctga cggtgtaatt cttttagtag atgcttttga aggaccaatg
    10141 ccacagacac gctttgtttt gcagaaagca cttgcattag gacttaagcc aatcgttgtc
    10201 atcaacaaag ttgataaaga aaactgtcgc ccagatgaag tacatgaatc ggtatttgat
    10261 ttgttttttc atcttgaagc aacagaagag caacttgact ttccaacatt atatggtagt
    10321 agtaaacatg gttggatgag cacagactgg cgcaaagaaa catcagacat aacagcttta
    10381 cttgatgcca ttatagagaa aacgcctcct gcaaaatcgc ttgaaggcac acctcagatg
    10441 ctcatcacat cgttagatta cagcaactat gttggccgta ttgccattgg cagaattgtg
    10501 agaggaacat tgaaagaaaa tatgcctgtg acgttgatga aacgtgatgg tagtgcaaaa
    10561 aaatcaagaa taaaagaact gtttgttttt gaaggattag gcagaaaaaa agttgacagc
    10621 gtaagcagtg gcgacattgc agctattgta ggtgtagatg attttgaaat cggtgatacc
    10681 attgcagatt ttgaaaaccc tgaagcattg aaatcaattg cagttgatga acccacaatg
    10741 agtatgcttt ttaccatcaa caacagtccg ctgtttggta aagaaggaaa gtttgttacc
    10801 tctcgccacc ttcgtgatcg tctttataaa gaaacagaaa agaatctggc catgcgtgtt
    10861 gaagaaacca attcaccgga tgcatatctt gtttatggac gtggtattct gcacatttct
    10921 attcttgttg aaaccatgcg cagagaaggc tatgaacttc agttaggaca gccacaggtt
    10981 ttgtacaaag aaatagatgg tattcgttgc gagccggtag agcatttagt tgttgataca
    11041 ccatcagaag tagcaggtaa agtgattgaa cttgccacac agcgtaaagg agaacttaca
    11101 agcatgttgc ctaaaggtga tttacaaaga cttgaattta atattcctgc aagaggatta
    11161 attggtttaa gaaatgcagt acttactgca actgcaggag aagcaattat ggcacatcgt
    11221 ttcaattctt atgagcctgc taagggtact attcccggaa gaaatatggg tgtgctgctt
    11281 gctaaagaag caggaagttc aacagcctac tctattgata aattacaaga gcgtggagca
    11341 ttttttattg atccgggtga aacagtttat ggtggacaag ttatcggtga acatatccgt
    11401 ccgggcgact tggtggtgaa tgtatgtgaa ggaaaaaaat taaccaacat cagagcatca
    11461 ggcagtgatg acaatgcgcg tattactcca aaaataaaat tctcgttaga ggaagcaatg
    11521 gagtatattg agaaagatga gtatcttgaa gttactcctc agaatattcg catgcgtaaa
    11581 atttatcttg atgaaaatga gcgtaagcgc atgagtaaga aagcagaaat ggcatagtga
    11641 aataagttag gattgtataa gtttttttat tattattgta aaaattaaac aataacatac
    11701 aatgaaggca gtgcagaaaa agcaaaaagt aaaaatggag tttccaatgc ggtcttctcc
    11761 caaaatatta tacggattcc tatccactcc atcagggcta tctgaatggt ttgccgatga
    11821 tgtcactatt cgtgaaggtg tttacacttt ccattgggat ggcgatgagc agcaagccaa
    11881 aatgactcac aaaagagaaa atcaaatggt gcgtttcaaa tggattgatg acagcgatga
    11941 tgcttttatc gaatttgaaa ttacaaccga tgaattgaca tctgatgtat cactaatggt
    12001 atctgacttt gcagtagaag atgatattga agaaacacaa cgtttatggg acacacaaat
    12061 ccataatttg cgtcatctga ttggatcata gtgttacact aatatctcta tacattgttt
    12121 tgatattaga cttaatattt atcttacaaa taccccatta ctttataaac tgctattcca
    12181 aaccattcat gaaatagcaa gtcccaatca agtaacacct ccggtgatgg caaaaaaagt
    12241 ttatcaggtg taaacattat ctgacctgaa tgttggtcaa ctgcaaaagg caccacatca
    12301 attccttgtt tctgaaaaca aagcattgac ctccgcatat gaaatgccga tgtcactaat
    12361 aaaaatggtg gtctaaaatc tgattttcga ataatttcag cagtattttt cgcattttca
    12421 taagtgtttc ttgactgatt ttctgcaatc actttatctg caggaacacc tatgcgtatc
    12481 atctgattac gcagtaaatc tgcttcttta acatcagtat aaaccaaact gcccgaacca
    12541 ctggaaataa ttatatactt tgctttgttt ctataaaaaa gctccagcga ctgaacatat
    12601 ctatcggcac ctgaaccaaa aaccggacgt tgcatctgtc cgtttacata acgtatagca
    12661 ccacccatca ttattgccgc atcatattcc ttatgaattg aatctataga taccgactga
    12721 atttcccatg catgcattac taaattcagc acaaacggat tgctgaaaaa tagtattgct
    12781 cctattgccc aatataaaca cttccggctt cgtttcttat tctttgtaat aacagaaata
    12841 agcaacaaga ttacgatcca acttagtggc gataataaaa aatctaaaat ctttgaaagg
    12901 aaaaaaaata tgtgttgcat taaatcaagt tacaacaaaa aaatcagaca gatgagtaaa
    12961 caaaaatcag acagatgagt aaacttaatg gggaactttt acatcgcccc agtttattcc
    13021 tctcttaatt cgaagtagtt gcgtttccga aacattaaat cggtcagata ttttagataa
    13081 tgtctctcct ttttgaattc gtttctttat ttcagctact ttcttctcat tgagtttata
    13141 gcccgatgca ttttccggac gcttaccaat tcgcttcttc ttctctttta taactacggg
    13201 acttttcttc tgatgttcga tattatcttc ctgagtcatc cacttcaggt ttgaaaaatg
    13261 attattgctt ttgttataat caagatgtgc aacaatatca tgttttttag aaggttgctt
    13321 aacgaaacta tctgcaacaa gacgatgcac caatggagca tagttaattg tgcgctctaa
    13381 ttcatctttt cggaatacat ttctatacac ttcctttaag gtgctcaact ctgcagcaac
    13441 attatcaact ttctttttat tgctgttata ggcaggtgaa ttctttttgt aagattttaa
    13501 ttctgatttt acatcgcgca gtttacctgt tagttgttca atttttcttc gtttttggtc
    13561 caattgcttt tgtgcatcat cagctctttt cttaaaaagc ttaaacctca caatcggata
    13621 tccatttatc aaagttcctt tcagcactct gttttctgca attttggtgg aagttctgac
    13681 cctcccaaaa tttgatactt ctattttcag attattggta aacctaaacg gaggctttac
    13741 aagcgaccac ttttcattct taaaatcatt tttcattgtt tgaaaattta caattagaat
    13801 tcaattgtga tttaatgatt caataaattt aactgattaa aatggcaaat cgtctttata
    13861 atcttcctgt ggtaaatctg acatagatgg cattggcgat tccattgggg actctcctgc
    13921 agaagttctt tcaattttcc atgcatcgag cgatgtaaaa tacttaatct caccggatgg
    13981 tgatttccac tctctgcctt ttatattgaa ggaaaccgaa agctcgtcac ccggctgaaa
    14041 actctctatt aacctgcact tatcatttgc cagttgaaaa ataactattt gaggataggc
    14101 tgacttagca tctattgtaa gtgtaaattc tcttttgcga aatttgtctg ttactacctg
    14161 ttctgcaaat ttctgtttta atattcctgt tacctgaagc attttgaatt ttttttcaaa
    14221 agtaaaaata aatttaaaaa atcaaatttt ttagttttaa tgcattggcc ttattattga
    14281 agttttccat aagaatttct tcccgtagcg aaatatctat ctgagtaaat tcctgtttaa
    14341 tcattgctgc aagtgcctca cgcatagctt caggtgtatc ggctacagtg cacaactgtt
    14401 ccaatcctgt attttgaacc attggtgtat ttacgagggc aaaacgacca ctaaaaagtg
    14461 ctgaaagcag tttaagctta attcctgttg gctgaaaagt gggcagcaca tttatatgag
    14521 catccaaaac taaatcgtta atctgcttag tgtttaaatc acttagcaaa gtaatattat
    14581 tctttctttg tacagcatct cttaaattaa caccgggttt gctaccggca atcagcaagg
    14641 taaccggtga gtttttaaaa acttcttgta ctaaatagag agcagccata tcattctcac
    14701 caatagccag attgccgtga taaaaggcat aagctccttt gccaattctc gaatttactt
    14761 ttgtaaagga atgaaaagca ggtatgtaat gtgcattgcc atacttactt ttaaaatatt
    14821 gtgtgtcgtt aggcgaaatg gtggcaatca catctgcatt tgataaaatg ttttcgtaga
    14881 tttctaactt actcgattca ttataaaaat agtatctctt aaagatgttt gattctgcct
    14941 ttgctaagtt tttataatat tcatgttcta tattatgtgt cctgactatt ttctttcgct
    15001 tttttagtcg ctcatcagac aaataatgag tactatgtaa tccttcaaat agtattggat
    15061 gctcatcgga aagcaggttt ttaatcagtt gctctgacga gcgtgacaca acaatatatg
    15121 gttcttttaa aaacaactgc gatttggaaa catcacgttg atagtagtga acagattcac
    15181 ataactcttc aagaattttt gatgactgac gaccatattg aaatgcatga aggtgaattt
    15241 ttattccggc agaaacaaga gcacggagct tataaaacac atcaataact ccaccatagt
    15301 ttgcaggata cggtatgtca aaactgataa tatgtaaatg cttgccgcta tttttcacaa
    15361 tgtgttttaa agtctgtttt actaattcac agaagataaa attaaatcaa ttaatacttt
    15421 tttctctttt tgccagcata aatccttaga tgcaatacta caattttctt tatagctaac
    15481 ataaagttct ttttcgataa ctaaacgatt cattgcctct gctattgctt gtggagagta
    15541 ttgtggcaac acaatccccg ttttatatcc gttcacaatt gctgtaacct caggtaaatc
    15601 tgaagctata acaggaatgc ctgcatgaat gtaatcgaat agtttgtttg gcaaactgaa
    15661 tttataattg atgttggtgt ctttatccaa tgtaagtcct gcaaagcatg ctgacgttac
    15721 agtaattaat tcctcgcgtg aaattttagg gtgaataatc actttgtgtt ttacattatg
    15781 tttgatagcc aattcttgta actgatgaaa aacatctccc ccacctataa taagtaacat
    15841 ggcatctaca tctttcattg cttcaattgc ctcctctgcg cctctgtcaa cattaattcc
    15901 tgcaccttgc attataaaga ccttcttgta atcaggaaat ccgacagaac gcttccattt
    15961 cgctacatcg aaattaacgc tatctgttct atcaggaaca ttacgaacaa ctttcacatc
    16021 attaccatac tgttggtgat agagatttgc aatcgaatta ttgaccgtga ttacattttt
    16081 aagtttcggg aaaataaaag cctctaccct tgtccatact ttgcgtgcaa tatgattgtt
    16141 ctgcaattcc ggaactcctg taaagtactc gtgactgtca taaatcaaat tacatccttt
    16201 cagtcttgac actacataat tcggcaagag ggtgtctaag tcattcgcat aaagaacatc
    16261 agctttttta aaaattaaaa aaaagaaaag tctaatctga aacaatgcat aaaaaaaagg
    16321 tcctttattt attggcaagt taaatcgctt taccgataaa ccggtcatat cataaacagg
    16381 taaactaagt ggtaactttc gaccaacaat aagtatttga tagttaagct cagaaaatac
    16441 tgatgctgtt cttctaaccc gctgatcggt tgctatatca ttaattacag aaattataac
    16501 cttcttttgc gccattctta gttgatgtgg cgaaggtaaa actttgcttg ttaaatttaa
    16561 atcaggctaa ataatcttta agcaacttgt ttcgagagcc tgagcgcaat cttcttatag
    16621 ctttctcctt tatttgcctg acccgttctc ttgttaaacc aaacttagcc cctatttcat
    16681 ccaatgaaaa actatgcgaa acaccaatgc cataaaacaa tttaaccaca tcacgctcac
    16741 gctctgtcaa tgtttttaaa gagcgatcta tctctttacg aagtgattca ttcatgaggc
    16801 caaaatctgc atcgggtgca tcatcatttt gcataacatc aagtaaggtt gcctcttcat
    16861 taccggcaaa tggcgcatcc atgctgacat gtttaccagg tgatttcaat gtatctgaaa
    16921 ttttttcaat cggcacttcc atcatctctg ctatttcctc agctgagggc tcacgctcaa
    16981 actcttgttc taattttgag aatactttat ttatttttct taacgaacca acctgattta
    17041 atggtaatct gacaattcga gcttgctctg caatagcttg taagatactt tgacgtatcc
    17101 accaaacagc ataagaaata aatttaaatc ctctggtttc atcaaaacgt gatgcagctt
    17161 taataagtcc gagattgcct tcatttatca aatcgggaag acttaatccc tgattctgat
    17221 attgcttggc aactgacaca acaaatctca aattagctct tgttaattta ctcaatgctt
    17281 cctcatcacc ctcacgaatt cttcgagcta aattagcttc ttcctctgca gtaatcatgc
    17341 cttcgcggct aatttcctga aggtactttt ccaacgattg gctttctctg ttggttatgg
    17401 acttttgaat gcgcagctgt ctcataaatt gtttcggttt atcttacaaa gttaatagta
    17461 aaaaaatcat taatccaaaa aaacattcaa ccaaaatgca tcatttgttt aaaaagattt
    17521 caacaaactg aaaaaagtca ttacaggatt ttttgtgatt tcggaaaatg aatgattact
    17581 ttgtatcatc aaaaggggtg cttcctataa tgaatggaat gctgagatca tacccataac
    17641 ctgatcagga taatgcctgc gtagggatag gtaacattct tttattgcac ctgcaacttc
    17701 ttttgtgatg ttacttaaaa atgaaaaatg atgattaaga aattttcttt attgctttta
    17761 gcgttatgct taagcctcta ttctaatgca cagctcgatc tatcaggagt tgtgagtact
    17821 aatgatggta aaaaagtgga tcatgcagtt gttaccctta aagaatcgct gatacgtcat
    17881 ttttcagatg aaaaaggcaa gtttactttt tttaatctta aaccgggtaa atacactttg
    17941 caggtaaatc agcttggcta tcatgaatac agcagtgaaa ttaacctggc aagtaatcaa
    18001 catatagaag taatactttt caaaaaagca tttgtaactg acgaggtaat tattcagtca
    18061 ttaaaaatga aaaacaaaat acctattgca caaaacagtc tgaataaaaa tgaaattgaa
    18121 gaaatgaatt cagggaaaga tgtgccggaa attatggatc aactcacaag tgtcgtggtt
    18181 agcagtgatg caggtgcagg tgttggctac acaggaatgc gcgtgcgtgg tagtgatgcc
    18241 acaagaatta atgttactat aaatggtatt ccaattaatg atgcggagtc gcaaagtatg
    18301 tattgggttg atatgcctga cctgcttagc tcaaccaatc agatacaact tcagagaggt
    18361 gtaggcactt cagtaaatgg cgcagcagca tttggtgcaa gcatcaatat tcttacagac
    18421 aatctttcag aaaaaccatt tgcagaaaca aacttgagtt acggagcttt caatacaaaa
    18481 aaggtgaatc ttattgcagg caccggatta ctgaataatc aatttgcttt tgaaaccaga
    18541 ctatcttcca tacagtctga cggatatgtt gacagagcat catcaaattt aaaatctttt
    18601 tatttttcgg gaggatattt tggtaaaaag tcaatgttga aggcaatcat tttttcagga
    18661 atagaaacga cttatcagtc atggtatggt gtgccggagt caaggtataa aaataacatg
    18721 cagggaatga tggattatat ttcccgtaat ggcttagaca gtgaagacgc tacaaacctt
    18781 cttgaatcag gaaggaaata caattactat ttatatcccg gacaaacaga caattaccaa
    18841 caggacaatt atcagttgca cttctcacat cagattaaca acctgtttac tttgagtgtt
    18901 gctgctcacc tgacacgtgg taaaggctat tatgaggagc ttaaaaaaaa tgcaaagttt
    18961 agtgactatg gattacctcc tattcaaatt caggatacca taattactaa aacaaatctt
    19021 gtgagaagaa agtggctcga caattatttt tatggcttga tttactcttt gacttatgaa
    19081 aataaaaacc ttgaaataaa aaccggaggt ggcatcaatc aatatgatgg tgatcactac
    19141 ggtaaaataa ttaaaggtga cattattcca ttagcttcag aaaattacac ttactatcaa
    19201 aataatggat ttaaaagcaa tttcaatcac tggataaaag gaaccttaca cttgggggag
    19261 catttcattg catatgcaga cattcaatat cgctacgtac attatcggtt tacaggattt
    19321 gatgattttg ccaccaccac taatcaggtt gcagagtttc attttttcaa tccaaaagcc
    19381 ggcattactt ttttacgtaa tgaaaacgaa agtgcctggc tgtctatcgc tgttggaaac
    19441 aaagaaccat caagagatga tttcactgag tcaactgcaa aatcacgacc taaatcagaa
    19501 aatctcatag attttgaagc aggttattca atcgcaataa aaaaattaaa actgagtctt
    19561 aatgcctttt atatgcaata taaagaccaa ctgattttaa caggaaaaat caatgacgtt
    19621 ggcaactata ccagacaaaa cgtaaaaaat agtttcagaa gaggtttaga agcagaattt
    19681 aattatgatg tgggtaagtt cttagctgtt tcaggcaatg tagcattaag cgaaaataaa
    19741 attctgaact ttaaggaata tattgatcag tacgatgcag attttaacta tacaggtcag
    19801 cagaaaatta tctataaaaa aacagacatt gctttttctc cttcaataat tactgctgtc
    19861 ggtattgaaa taaggccgtt aaaaatgtta agcatcaaac taactgagaa gtatgttgga
    19921 aaacaatatt tagataatac ctcctcggag gaaaaatcat tgaaagcatt tgcctacacc
    19981 aatctttcaa tacattatac gtttaagatt tttaaaacca ttgaaactga agcaggagtt
    20041 gcagtatata acctgttcaa tgaaatgtat tcaagtaatg gttatactta tgggtactta
    20101 tatgatggta tgagggttaa cgaaaatttc tactatcctc aagcaggaac acataccatg
    20161 ctaactgcta aattcagatt ttaatttcac catcaaaaac attcatagca gaacctgtta
    20221 gcataacatt tttgtatttg ttagcttcaa cctggaaagt aacattcaga cttccaccac
    20281 gtgcaatcac attcaccgat aatttctttg ttaaaccgga ataatgtgct gctattgcag
    20341 atgcagttat gccggtgcca caggcaagag tttcatcctc taccccgcgt tcataagttc
    20401 tgatgtgtga tgctttgctt ttaaatgaaa caaaattcac attaatgccc tttgccttaa
    20461 aagtattgct gtttcttatt ttttttcctt ctttaacgac atcaatttta tctgtatcat
    20521 caacaaactt tacgtaatgc ggtgagccgg tattcagtat aaaatttcca ttgtgttttt
    20581 taatttccga aacatcagac atagatacac ttacgatagc actctttagt gcttttgctg
    20641 cactgacctt agcagaatgc aacccatcat aagccataaa caatgtttcc ctgccaatca
    20701 ttcctaactg tttactgaat aaaacagcgc agcgtgctcc attgccacac atactaccca
    20761 gccggccatc agcattaaaa tataccattt caaaatctga cttcagacta tgctgaatca
    20821 atattagtcc atctccacca ataccaaaat gcctgtcgca taacatttcc ctttgttgta
    20881 cttttaaaga agaatactta ccagatctgt tatctatcat aacaaaatca ttgcctgcgc
    20941 cctgatactt gctgaatttt agatgcattt ttattaattg ataaactata tcttaagatt
    21001 aaacgttgta aaactacgtg aatattctga aacattgtta acgattgtta acatgcgctt
    21061 catagtcatt tcactgatag tgatataact tcgttgtcga aataaaacgt aaaacataca
    21121 agattataag taatgataag acagattaaa aaaacagccg gaata
//
LOCUS       DAOOOI010000040        21148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_485_length_21148_cov_27.584175, whole genome shotgun sequence.
ACCESSION   DAOOOI010000040 DAOOOI010000000
VERSION     DAOOOI010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 21148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21148
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_485_length_21148_cov_27.584175"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1928
                     /locus_tag="PKX59_06520"
     CDS             <1..1928
                     /locus_tag="PKX59_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ90557.1"
                     /translation="RGKGRAATGYNSIASNFNQWWQRQLDIKELKDYKNPDGSQRAWN
                     MNAEDDPSPLYWDNPYWVSYENYETDQRNRLYGGLTFDYDIGKGFSIAVSGKTDYYND
                     NRQERVAVGSAVATAIPAYSENSITFNENNYDMILSYKHRLGDDLDFNGFVGVNRVDQ
                     KFSNKFVSTQGGLNVPNFYNINNSTSSELIYSSQVRQLRKNSLFASASFGYKSFLYLD
                     VTGRNDWSSTLPEGKNSYFYPSITGSFVFSEKLDFNWLSYGKLRAGWAQTAIDPVPYR
                     STLTSPSMSDNFNGLPTSVIPGTFNNTDLRPEMTSGFETGLELHFLQKRISLDVAYSS
                     TRSKDVIFNVQQSGASGIDFKIYNAAELTNKTVELMINVIPVKLKNGLEWGVGMNWAK
                     TNNKVVKLFTDELGNETENVQIGTAPFSATLQMRKGMEASQIVGYDFVYDDKGNKVVG
                     ADGFYLRSETVKPLGSVLPDYTGGVNTYVSFKGIRLSGLIDFQKGGKVFSLTNVWGKY
                     SGIFAETAEGDIRENGLVNEGVTETGEQNTTVIAAVDHYFQDGGYVITAADVYDASFV
                     KLREMTLSYELPQSLFKKAIRGVSVGVYGRNLAILHKNVPNIDPEAGLSSGNVQGFEG
                     GQLPTARIFGVNLNVRF"
     gene            1947..3473
                     /locus_tag="PKX59_06525"
     CDS             1947..3473
                     /locus_tag="PKX59_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOZ90558.1"
                     /translation="MIKFKINQKAIATVMVFSLLLGSCKKNFESINEDPNNPKEVPNA
                     YLLTGAMRGLMDNTIDVWWGGNVGNQLGQYWSSNQYSSESRYLYRSNVTNSYWRYFYG
                     GVQNDQLVAVGGLYELSQIVKKCKEDSIEASRDGYVPNQIAVATIIRVWTFQNMTDIW
                     GDIPYSEALQADGNKTPKYDKQEDIYNGLLSEIDDAIGMINDAESGPNGDVVYNGDMT
                     AWKKFANSLKMRLALRIVDRNAGKAQSEFEAAAAGAFTSNSDNALLQYGDFPSGNPIY
                     YNRYISGRNDYASSNTFLDSTLTPLNDPRRECFFVPATATGLWTGEVYGLSEANAAQT
                     PNSRVAQRSPLVLSASLPGIFMDYAQVEFMLAEAAERGWAVTGSAESHYDAGITASIS
                     FWTALNGAAASAGDIAAYIAQPTVNYQTAGGTWQQRIGVQKWIALFDQGIQGWVEWRR
                     LDFNKLMMPADGVIDGTGIPLRMKYPVLEQTLNSASYSAAVGAQGADEQDTRMWWDVN
                     "
     gene            3584..4969
                     /gene="asnS"
                     /locus_tag="PKX59_06530"
     CDS             3584..4969
                     /gene="asnS"
                     /locus_tag="PKX59_06530"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOZ90559.1"
                     /translation="MSHSYIKALLNTEPTGQIVKAKGWVRTKRGNKAISFIALNDGST
                     IHNIQVVAENNNFDESLLKNITTGACIAVEGKLVASQGQGQKVEMQATQIEIYGTADP
                     ETFPLQKKGHTMEFLRDIAHLRPRTNTFGAVFRIRHTMAYAINHYFHTNGFFYIHTPI
                     ITGSDAEGAGEMFRVTTLDMNKPPRNEDGSIDYSQDFFAKETNLTVSGQLEGELAAMA
                     LGKIYTFGPTFRAENSNTPRHLAEFWMIEPEMAFFDLNDNMDLAEDMLKYLVRYALDN
                     CMDDLEFLNNMYDKELIGRLRSVIENPFKRLTYTEAINVLEPNNSKFEYKVSWGADLQ
                     KEHENFLVEHFKTPVILTDYPASIKAFYMKQNDDGKTVRAMDILFPWIGEIIGGSQRE
                     ENYDKLVSRMKAMHIPEKEMWWYLDTRKFGACPHAGFGLGFERLMLFVTGMNNIRDVI
                     PFPRTPRNAEF"
     gene            complement(4980..6203)
                     /locus_tag="PKX59_06535"
     CDS             complement(4980..6203)
                     /locus_tag="PKX59_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ90560.1"
                     /translation="MTKTRNAFVAWKHKDYRLFLIAKVCLTIAIQMQSIILSWLIYEM
                     TKDPLSLGIIGLIEAIPALSVALFGGHLADRLSRRKLLIWSCAITLSASAFLAIYVYD
                     FGKISTWPIYLTIFLIGLARGFHAPTQAAFWGQLVPKEHYVNASTWNSSLWQVAAVIG
                     PAMGGFFYGWIGVFYSSLVVCAFIIVTMICYLLIKDRHVDYHNKTESILQSLKTGVNF
                     VFKNQFIISAISLDLFAVLFGGAVALLPVFADQVLHVGAQGLGLLRAAPAVGSVVMAI
                     FLAFHPPRKDAGYKMLASVVGFGLCMIGFALSGSFLLSLFLLFLSGVFDNVSVVIRST
                     IIQSFTPDEMKGRVSAVNSIFVGSSNELGAFESGVMAKLMGLIPSVLFGGGMTLLVVL
                     ITFIKAKKLKELNLP"
     gene            complement(6262..6483)
                     /locus_tag="PKX59_06540"
     CDS             complement(6262..6483)
                     /locus_tag="PKX59_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOZ90561.1"
                     /translation="MYWTLELASHLEDAPWPATKDELIDYAIRSGAPVEVVENLQELE
                     DEGESYESIEEIWPDYPTKDDFFFNEDEY"
     gene            complement(6597..7148)
                     /locus_tag="PKX59_06545"
     CDS             complement(6597..7148)
                     /locus_tag="PKX59_06545"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOZ90562.1"
                     /translation="MKIYTKTGDSGKTSLIGGTRVSKHHLRIDAYGTVDELNSTIGLL
                     KDLAEEKDLCKEILAEIQDRLFTIGSSLAADPEKSKMKIPDLNTEDITLLEKEIDRMV
                     AALPPLKSFVLPGGHLLNSHTHVARCICRRAERLATGLSESEWVEPMVLIYLNRLSDY
                     LFMLARHVCFVRNITETPWRPRV"
     gene            7373..8953
                     /locus_tag="PKX59_06550"
     CDS             7373..8953
                     /locus_tag="PKX59_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOZ90563.1"
                     /translation="MFKKVGLVLLLLAYCRSTSAQNIEIQKLGALLQMIDYYYVDTVN
                     KPKLVDDGIRAILKDCDPHSQYIPASELKEMNEPLVGKFEGVGIQFNILDDTIMVTQT
                     IAGGPSEKLGIRPGDRIVNIDGKKVAGVKITNKDVISKLRGDKGTKVKVDMYRRGEAE
                     LLTFDITRDKIPLFSVDASYKIEPDIGYIKISRFADNTVEEFKEALAKLKSQGVKSLI
                     LDLTSNGGGYLNRAHQLADEFLSDGKRIVYTEGRSQSREDYFSTAAGGWEKGKLVVMI
                     NEYSASASEIVSGAIQDWDRGLIVGRRSFGKGLVQKPYSLPDGSAVRLTVARYYTPSG
                     RCIQKSYQEGDEEYSDDISNRFKHGELYHADSIKFNDSLKYTTNAKRVVYGGGGIMPD
                     VFVPLDTMFSSKYYSDLIRKAALSEFALTYADNNRSKLKSAYTTVENFKKNFNVDDKM
                     MNDFIAFAEKKEVKKDEEGLKRSGTMIKAQIKALIARDIFNFGAYFYIINDSDDTFNK
                     AVESIKDNTFSKMKIANN"
     gene            complement(8959..11703)
                     /locus_tag="PKX59_06555"
     CDS             complement(8959..11703)
                     /locus_tag="PKX59_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90564.1"
                     /translation="MKKLILSFFFLTIIQQAKAENNQMPISAFVIQWTSAWCGSNSGA
                     LIAQVNGGVAPYSFFWNGIPGTDTLLGVPAGIYDLLVIDAQGDSAQSSGMVNAQVEVP
                     FSVISIAMSNPGSNNGAFQVVIYPGPSANDIPQGTYNYNLYDSTALNLLATHTFSYYV
                     GGAIDPYYIFDSLPAGKYYVHGVAPFGCYTQVEFRIKELPTINPTFSVSDACNGSPTG
                     NLSINLHPYPAINNNTFNSNLISSYTGAGNAVTSKYKTIITDSVNNTVGYRAVQDSLL
                     DYKNLLPGNYTLKVFTGDTIYSQGIPNIQDSILIYSTPFTIVNDPNCSLVKGRVFGDY
                     NSNCSYGFGDILFSGTLVELNPGGYSSVTNAAGEFFIPVVNGTYTLKQYAPYQFAQVC
                     PDTTTFTINITTPGTLVDVQVADSVNVNPDLQVGISGNQPRPGFNHHYKIDVRNLTPK
                     IIPAQTLTVSYDTTLNFVNAVPAPATNSNGVLMFTGISLPPFGTASFQINTVVPSTTP
                     LGTVLNASALITTVVGETIISNNIDSISQTVAGSFDPNDKAVMPHGFDAAGYITAAQQ
                     LKYTIRFQNTGTDTAFAVMIADTLPLSLNKYSLSVLNTSHPFTYEFASGNVVKFHFDN
                     ILLPDSNSNEPASHGYIKFKIDQIPGNAAGTVIQNNAAIYFDFNSPVITNTVTNTVFD
                     CNQMATVTLSNSTICENDSIQANSALLFDMNSEWLLDGTSYSNDSSFYLTGLSAGNYS
                     LTFVASNLVCSQSIINNIIVNPIPPQPFITNIANTLQSSSVSGNQWLLSGLPIGGATA
                     TSYIPTATGFYSVMVTDVNGCTKISDSIYILITSINNIGSANICIYPNPVKDELIISG
                     LTNGENVLTVFDAVGKVVLSVITSNTKEKIKTTFTKGIYFISIKNALHQIQMLRFTKE
                     "
     gene            complement(12022..12441)
                     /locus_tag="PKX59_06560"
     CDS             complement(12022..12441)
                     /locus_tag="PKX59_06560"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ90565.1"
                     /translation="MTTKNIQTIQIESKPESIHIIERLVDDLKGEHNIHEDAYGNILV
                     AVTEAVNNAIQHGNKYNPNLKVKISYEVESDTISFAITDQGKGFDYYNLPDPTAPENL
                     EKPTGRGVFLMKHLADQIIFSNNGSSVELFFKTGHVA"
     gene            complement(12447..15785)
                     /locus_tag="PKX59_06565"
     CDS             complement(12447..15785)
                     /locus_tag="PKX59_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90566.1"
                     /translation="MTGNYRLLIEKLDEFIRKYYRNQVIRGSIYATGITIGFFLFISL
                     AEYFAWMGSSLRTALFYIYVFTVLAVIIRLIVIPLFKIWKLGKTLSYEQAATIIGNHF
                     TDVKDKLLNTLQLNELSENNHNELVQAGIDQKIQNLKPIPFTGAIDFKKNRKYLPLAL
                     IPAGTLIILLFAAPNVIKESTSRLVNYNVAYKKPAPFNFILLNKNFNVIEQNDFTLNF
                     KVEGQELPDQVYVTYEGRQIKADKEDKQHFTFTFHRIIKSTSFQLMADGFYSENYELK
                     SVPNPLISAFKIDLNYPAYLQKKNETLNNTGDLLIPAGTIVKWTFNTVNTSVFKLWFN
                     DSLTPLVSDNNQYTYSKRMLSGANYSIATANNFITNKDTLEYSINVIPDQYPIIQVEQ
                     QNDSSSLQRIFFKGTVKDDYGFSKLAFHYRFVKSKNDSSAHSDKLNTEILTINKNNNQ
                     TDFYHFWDGALHGVVPGDEVEYYFEVWDNDGVNGPKSAKSQSLIFKAPTLRELAENTE
                     KSNDEIKKDLQESLKQASKIQKQITDAARKLTEKKKTDFEDRKRIEDLLKQQKELDEK
                     LDKIKNEQQQNLAKQNEFKNNESQLAEKQQQIQELFDRLQNPELKELMKQLEQMLSQL
                     DKQSVQEALDKMKLNNKDLEKQMDRTLELFKQLEVEQKLKNTLENLDKLAEDQKKLSE
                     QSMDKNSDTEKLKEQQKDLNKQFEDIKKDIAALEKKNQELEFPQELANTEQEQKDIDQ
                     EQQNSEQQLNENKKSNAGKSQKNAAEKMQNMSQKMKNQMAQSESEQSEEDMASLRYLL
                     ENLLRFSFEQEALMQALKTTDVNNPQYVKMGQRQKKLQDDLKIIEDSLFALSKRVIQI
                     QQTVNTEISNIQSNIQKSIAFLQDRQVPVARSSQQYIMTSANNLALLLSEALQQMQMQ
                     QQQQKQGNGSCKKPGKGKPGPSAEKLKMMQQQLSEQMKKMKGKLEGMQKNGGKGDKGM
                     SEELARMAAQQEAIRNELNQLNQQENKDGKNSMGNLGNLANQMEQNERDIVNKKITQE
                     TLKRQQEILTRLLEAENAQREREQDQTRKAEQAKEQFLRNPAAFEEYKKLKEKQTELL
                     NTVPPGLNTFYKNLVNNYFQGLQF"
     gene            complement(15957..17294)
                     /gene="accC"
                     /locus_tag="PKX59_06570"
     CDS             complement(15957..17294)
                     /gene="accC"
                     /locus_tag="PKX59_06570"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOZ90567.1"
                     /translation="MFKKILIANRGEIALRIIRTCKEMDIRTVAVYSTADRESLHVRF
                     ADEAVCIGPPPSKDSYLNIPNIIAAAEITNSDAIHPGYGFLSENSKFSAICAKNKIKF
                     IGATPEQIDGMGDKSNAKDTMKKAGVPTIPGSEGLLTSVEEGLKIAAEIKYPVILKAT
                     AGGGGRGMRIVWKDEEFAAAWDSARQEAGAAFGNDGMYLEKYIEEPRHIEIQIAGDQY
                     GKACHLSERDCSVQRRHQKLVEETPSPFMTKELREKMGEAAIKAALAVKYEGVGTVEF
                     LVDKHRNFYFMEMNTRIQVEHPVTEEVINYDLIKEQIKIASGEPISGKNYYPTMHAIE
                     CRINAEDPYNNFRPSPGRITNLHVPGGHGVRVDSHVYAGYTIPSNYDSMIAKLITIAQ
                     TREEAINTMERALSEYVIEGVKTTIPFHIQLMKNEDFRKGNYTTKFLESFKLL"
     gene            complement(17369..17854)
                     /gene="accB"
                     /locus_tag="PKX59_06575"
     CDS             complement(17369..17854)
                     /gene="accB"
                     /locus_tag="PKX59_06575"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOZ90568.1"
                     /translation="MNKKEIEELIRFVAKAGVSEVHLEQKDFKITIKNPTKEQIVHVA
                     PAVQPVMQMPAPAQPQQVIAAPAVAAAPAAKTAGDDSKYKTIKSPMIGTFYRTPSPDK
                     PNFVNVGDEVKEGQVICIIEAMKLFNEIESDIKGKIVKVLVEHSTPVEYDQPLFLIDP
                     S"
     gene            complement(18041..>21148)
                     /locus_tag="PKX59_06580"
     CDS             complement(18041..>21148)
                     /locus_tag="PKX59_06580"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90569.1"
                     /translation="LKIGINLTHTYSGDVIAKVTSPGGVITVFDRLGVPASSFGNGQN
                     FGGYYEFDVSAGSVLPESNSGPTTIPAGTYAPSNTNGSINTNWSGISFPMNAGGNWVL
                     NMSDRASGDVGTIVSWTVTIMTSSGITYTWTSTPNGFNSTDENPSATVTGTTTYNLAA
                     TANGCTANTTVGVSATPGPLAIASSNAPVCDGNDINFIADNLAGGQGSGNSYQWYDPN
                     NLPFSSQQNPNIFSATSAYNGTYTVVVTNQYLCTATTTVQVDVNPNPVINVSTQINVN
                     CFGESNGSITTNTSNGTGPYSYTYDFVNFNTTGEFDLLPAGSYTIYVSDANACQNTVT
                     ATITEPALLQFTKTYTDQQCPSTFGSITMNATGGTTPYMYSYDNGSTYVNNNANLNLT
                     AGSYNVMVQDAHMCATSTQQQDLILVNTPSTAALSVSSQNGTELCIGGSTTLSAVGGS
                     LGSTADWKWYKTNCGTTPVGSGPSITVNPSSSTSYYVRAEDVCGATPCADLSLAITVG
                     VPSAAVTVPMTGMPLNACVGTTANLSINAVNNTTQYVWDAPIGSYFDGNPLNVSPYTT
                     SSPNVQVTFGSPNGSFYNVGVQAANACGSTLRKIQKVRGIVSVPATVLGPQTVCENTS
                     NVAYNIAPITGASQYQWSITGDATVTGNGTNVNIDFGPTWNGGTLCVAAQTSCYTSAS
                     KCISLSKSAGIPNNLSGNFTACPNTSQTYSVTPLQGAAVYNWTLPTGASGSSNNNSIN
                     VTFGNGYNNIGNVCVSVTSICGVTSALKCKTVAPGIPMVPPAITGITNGLCNQSVAYS
                     TPIQTGTSYNWTAPAGANVFGQGNNSVNIQFSTMTTGQVCVVASNTCGTSAPRCVAVK
                     GAPNPPTDIIALPSSWCANTQGIELTANISNTTGSYTLDWGYPSAPVATYVAGGGNSN
                     SLTLDWGTGNGVIAITASNACGSATKTYNATTNCRESEELTAANFNMYPNPASDVVNI
                     EFWSDKDGMQKDLKVLDLSGRTVMVQKIQTISGRQSVQISLAQFAKGVYMIELNYQRN
                     KLVIE"
BASE COUNT         6349 a         3871 c         4171 g         6757 t
ORIGIN      
        1 caagaggtaa aggtcgtgct gcaacaggat ataacagtat tgcctctaac ttcaatcagt
       61 ggtggcaacg tcagctcgat attaaagagt tgaaagatta taaaaaccct gatggctcac
      121 agcgtgcatg gaatatgaat gcagaagatg atccatctcc actttattgg gataatccat
      181 attgggtttc ttacgagaat tatgaaaccg atcaacgtaa ccgtttatat ggaggtttga
      241 catttgatta tgatatcgga aaaggttttt ctatagcagt atccggcaaa acagactact
      301 ataacgacaa ccgtcaggag cgtgttgcag taggaagtgc agttgcaact gcaatacctg
      361 cttattcaga aaacagtatc acattcaatg aaaataacta tgacatgata ttgtcataca
      421 aacatcgttt aggtgatgat ttagatttta atggttttgt tggtgttaac agagtagatc
      481 agaaattcag caataaattt gtttctactc aaggtggttt aaatgttccg aatttctaca
      541 acatcaataa ctcaaccagc agcgaattaa tctattcttc tcaggttaga caacttcgta
      601 aaaattcgct ttttgcaagt gcatctttcg gatataagag tttcttatat cttgatgtaa
      661 cagggcgtaa tgactggagt tctacattac cagaaggcaa gaattcttac ttctatccat
      721 caattacagg aagttttgtt ttctccgaaa aacttgattt caattggttg agctatggta
      781 aattgcgtgc aggatgggca cagacagcta ttgacccggt tccttaccgt tcaactttaa
      841 cttcaccaag tatgtctgac aactttaatg gtttgccaac aagtgttatc ccaggtacat
      901 ttaacaatac cgatttaaga ccggaaatga cttcaggttt tgaaactgga ttggaactgc
      961 atttcttaca gaaacgaatc tctctcgatg ttgcttatag ctcaacacgt tcaaaagatg
     1021 taatcttcaa tgtacaacag tcaggtgcaa gtggtattga ttttaaaatc tacaatgcag
     1081 ccgaattaac caacaaaaca gttgaattga tgattaacgt tattcctgtt aaattgaaaa
     1141 acggattgga gtggggcgtt ggtatgaact gggcaaaaac caacaacaaa gttgttaaac
     1201 tgtttacaga tgagttaggt aatgaaaccg aaaacgttca gattggaaca gcacctttct
     1261 ctgcaacact tcaaatgcgc aaaggcatgg aagcatcgca gattgtaggt tatgattttg
     1321 tttatgatga caaaggaaac aaagtggttg gtgcagatgg tttttattta cgttcagaaa
     1381 cagttaagcc tttaggctca gtattgcctg attataccgg tggtgttaat acctatgttt
     1441 cattcaaagg tatccgcctt tcaggtttaa ttgacttcca aaaaggcggt aaagttttct
     1501 ctcttaccaa tgtatgggga aaatattcag gaatttttgc tgaaacagca gaaggtgaca
     1561 tccgcgaaaa cggattggtt aacgaaggtg taactgaaac aggtgagcaa aacactacag
     1621 ttattgctgc ggttgaccac tattttcagg atggtggtta tgtaattaca gcagctgatg
     1681 tatatgatgc atcttttgta aaattacgcg agatgacttt atcttatgaa ctacctcaat
     1741 cactgtttaa gaaagccatt cgaggtgtat ctgttggtgt atatggtcgt aatcttgcta
     1801 tccttcataa aaacgtacca aacattgacc ctgaagcagg tttgtcaagc ggaaacgtac
     1861 aaggatttga aggtggtcag ttaccaactg caagaatttt tggtgttaac ctgaacgtaa
     1921 gattttaact ttaaaaaaga tagatcatga taaaatttaa gataaatcaa aaagcaattg
     1981 caacggtgat ggtattttcg ctgttgctgg gttcatgtaa aaagaatttt gaatccataa
     2041 acgaagatcc gaataacccc aaagaagtac cgaatgctta tctgttaaca ggtgcaatgc
     2101 gcggcttaat ggataataca attgacgtat ggtggggtgg caacgtagga aatcagcttg
     2161 gtcagtattg gtcatctaat caatattcct cagagagtcg ctatctgtac cgttctaatg
     2221 ttaccaactc ctattggaga tatttttatg gtggcgttca aaacgatcag ttagtagctg
     2281 ttggcggatt gtatgaattg agtcagatag ttaaaaagtg taaagaagat tcaatcgaag
     2341 catcgcgtga tggctatgtt cccaatcaga ttgctgtagc aacaattatc cgtgtttgga
     2401 ctttccagaa catgactgat atttggggtg atattccata ttcggaagcg ctacaagccg
     2461 atggtaataa aacaccaaag tacgataagc aggaagatat ctacaatggc cttctaagcg
     2521 agattgatga tgctatcgga atgattaacg atgctgaatc aggaccaaat ggcgatgttg
     2581 tttacaatgg cgatatgact gcatggaaaa agtttgctaa ttccttaaag atgcgtttag
     2641 ctttaaggat tgtagatcgt aatgctggaa aagcacagtc agagtttgaa gctgctgctg
     2701 ctggagcatt tacctctaac agcgacaatg cattgttgca atatggagat ttcccaagcg
     2761 gtaacccgat ttattacaac cgttacatct ccggacgtaa tgactatgca tcaagcaaca
     2821 ccttccttga ttctacttta acaccattga acgacccacg cagagaatgt ttctttgttc
     2881 ctgcaactgc taccggacta tggacaggtg aagtttatgg attgtcagaa gcaaatgctg
     2941 cacaaacacc aaactcacgt gtagcacaac gctctccttt ggttttatct gctagcttac
     3001 ccggtatttt tatggattat gcacaggtag aatttatgct tgcagaagct gctgaaagag
     3061 gatgggctgt tacaggctct gcagaaagtc attatgatgc aggtattact gcaagcattt
     3121 ctttctggac tgcacttaat ggtgctgctg cttctgccgg tgatattgct gcatatatag
     3181 cacaaccaac tgtaaattat cagactgccg gaggtacttg gcaacaacgt attggtgtgc
     3241 agaaatggat agctttattt gatcagggta ttcagggatg ggttgagtgg agaagacttg
     3301 actttaataa actgatgatg cctgccgatg gtgttattga tggtactggt attccattaa
     3361 gaatgaaata tcctgttctt gaacaaacac ttaacagtgc aagctacagt gctgcagttg
     3421 gtgctcaggg cgctgatgaa caagacacac gcatgtggtg ggatgtaaat taatataatg
     3481 atttgaacta cttataaggg cggaggttta ctccgccttt tttgtttatg ctttcttagc
     3541 ataccgttgt ttctgataac tttgcggcaa atattatcgc ataatgagtc acagttatat
     3601 taaagcatta ttaaatactg agccaacagg tcagattgta aaagcgaaag gatgggtgcg
     3661 tacaaaaagg gggaataaag ccatttcatt tattgcctta aatgatggca gcactattca
     3721 taatattcag gttgttgcag agaataataa ctttgatgaa tcgttactga aaaacataac
     3781 tacaggtgca tgtattgctg ttgaaggtaa gcttgttgcc tcacaaggac aggggcagaa
     3841 ggtagaaatg caagccactc aaattgaaat ttatggcact gccgaccctg agacatttcc
     3901 attgcagaag aaaggtcata ctatggaatt tcttcgtgat attgcacacc ttcgaccacg
     3961 aacaaacact tttggtgctg ttttcagaat aagacataca atggcctatg ccatcaatca
     4021 ttattttcat accaacggat ttttttacat ccatacacca atcattacag gcagcgatgc
     4081 tgaaggtgca ggagaaatgt tcagagtaac aacattggac atgaacaagc caccacgcaa
     4141 tgaagacggt agcattgact atagtcagga tttttttgca aaagaaacaa acctgacagt
     4201 tagcggacaa cttgaaggtg aactcgctgc aatggcattg ggaaaaattt acactttcgg
     4261 gcctaccttt cgtgccgaga acagtaatac gccacgccac ttagcagaat tctggatgat
     4321 agagcctgaa atggctttct tcgacctgaa tgataatatg gatctggcag aagatatgct
     4381 caaatattta gttcgttatg cactcgataa ctgtatggat gatttggagt ttctcaataa
     4441 tatgtacgat aaggaactga ttgggcgttt acgcagtgtc atcgaaaatc cttttaaacg
     4501 tttaacctat accgaagcaa taaatgtttt ggaaccaaac aacagtaagt tcgaatataa
     4561 agttagttgg ggtgccgacc tgcagaaaga acatgaaaac tttcttgtag aacattttaa
     4621 aacacctgtt attttaactg attatcctgc ttccatcaaa gcattttata tgaagcagaa
     4681 cgatgatgga aaaacggtca gagcaatgga tattttattt ccatggatag gtgaaattat
     4741 tggtggctca cagcgcgaag aaaattacga taagttagtc agccgtatga aagccatgca
     4801 cattcccgaa aaagaaatgt ggtggtatct cgacaccaga aaatttggtg catgtccaca
     4861 tgctggtttt ggtttaggct ttgaacgttt gatgcttttt gtaaccggta tgaataacat
     4921 ccgcgatgtc attccatttc cacgcacacc acgcaatgca gagttttaat tttttctgat
     4981 taaggtaaat ttaattcttt aagttttttt gcttttataa aggtaattaa taccacaagc
     5041 aaggtcattc ctccgccaaa gagtacagaa ggtattaagc ccatgagctt tgccataaca
     5101 ccagattcaa atgcccccaa ttcatttgat gagcctacaa aaatactgtt cactgccgat
     5161 actcttcctt tcatttcgtc aggtgtaaaa ctttgaataa tggttgagcg aatcaccaca
     5221 cttacattat caaaaacacc gctcaaaaac aacagaaaaa gagagagtaa aaaactgccc
     5281 gaaagtgcaa accctatcat gcataatcca aatccaacaa cgcttgccag cattttatag
     5341 cctgcatctt ttcggggtgg atgaaatgct aagaatatgg ccattactac tgagccaaca
     5401 gcaggtgcag cacgcaacaa accaagtcct tgtgcgccta catgcaaaac ctgatcggca
     5461 aaaacaggaa gcagcgccac agcgccacca aaaagtactg caaacaaatc gagagagatg
     5521 gctgaaatga taaattgatt tttaaaaaca aagttgactc ctgtttttaa agattgtaaa
     5581 attgattctg ttttgttgtg atagtcaaca tgcctgtctt taatcagtaa ataacaaatc
     5641 attgtaacaa tgataaaagc acagactacc aacgaagaat aaaatacacc aatccaacca
     5701 tagaaaaaac cacccattgc aggaccaata actgctgcca cctgccaaag gctgctgttc
     5761 catgttgatg catttacata atgttctttg ggcactaact gaccccaaaa tgctgcctgc
     5821 gtaggtgcat gaaatccgcg tgccaatcca atgagaaaaa tagtaaggta aattggccag
     5881 gtagaaattt tgccaaaatc ataaacataa attgccagga atgctgaagc agaaagggtg
     5941 atggcacaac tccatattag aagcttgcgt ctgctaagtc tgtcggccaa atgcccacca
     6001 aacaaagcaa cactcaatgc aggaattgct tcaatcaaac caattattcc taaagaaagc
     6061 ggatctttgg tcatctcata aatcagccag ctgagaataa tactctgcat ttgaattgct
     6121 attgtcagac aaactttagc tatcagaaac aaacggtaat ctttatgctt ccaggctaca
     6181 aatgcatttc ttgtttttgt cataaaaaaa ttaaggccaa gcatttatgc tcagccttat
     6241 tttcagatat catttataag attaatactc atcttcgttg aaaaaaaagt catccttagt
     6301 tggataatca ggccaaattt cttcaatact ttcatacgat tcaccttcgt cttcaagctc
     6361 ctgaagattt tcaaccactt ctacgggtgc tcctgaacgg atggcataat caatcaattc
     6421 gtctttagta gcaggccagg gggcgtcttc aagatgcgat gctaattcca gtgtccaata
     6481 cattacaata ttcttattaa aaaattaaac taattgtgcc ttaattttta agggctgcaa
     6541 aaatatgtaa attaaattgg aaaaaacaag tgtttttttt aatagttatt aagattctaa
     6601 actcttggtc tccagggagt ttctgttata tttcggacaa agcatacatg gcgtgccaac
     6661 ataaaaagat agtcagagag cctatttagg tagattagta ccattggttc tacccattcc
     6721 gactcgctca gaccagtagc cagacgctct gccctgcggc aaatacaacg tgccacatga
     6781 gtgtggctgt ttaacagatg accaccgggc aaaacaaatg acttcagtgg tggcagtgcg
     6841 gctaccattc tgtcaatttc cttttctaac agcgtaatgt cttctgtatt taaatcagga
     6901 attttcattt tcgacttttc cggatcagca gctaatgacg agccaatggt aaacagtctg
     6961 tcctgtattt cagccaatat ttccttacac aggtctttct cttcggcaag atccttcagt
     7021 agccctatgg tagaatttag ctcatcaaca gtgccatagg catcaatacg cagatggtgt
     7081 ttgctgactc ttgtaccgcc aatgagtgat gttttgccgc tgtcaccggt tttggtatat
     7141 attttcattg attaatcaga taaataataa tttttgtaca acaaacctat taacattttg
     7201 ttactgaaaa gaagtgacag aagttaaaat gttaacagct ttggctgttt tctttgatag
     7261 ttaaattaaa gtttgttaaa ctatcttaca gcatgttgct taccatagat cgcatggatt
     7321 aattttggta tgtttacttt ctgtaaaaac ggatatttaa agttcaatta taatgtttaa
     7381 aaaagtagga ttagtattat tactgttagc gtattgccga agtacatcag cacaaaacat
     7441 agaaattcaa aagttgggtg cattgcttca gatgattgat tattattatg tggacactgt
     7501 taacaaacca aaattggttg acgatggcat cagagctatt ttaaaagatt gcgacccgca
     7561 ctcacagtat attccggcaa gtgagttgaa agaaatgaac gagccattgg taggtaagtt
     7621 tgaaggtgta ggcattcaat tcaatattct tgatgatact attatggtaa cacaaactat
     7681 agccggtggt ccatcagaaa aattaggtat cagacccggt gaccgtattg taaatattga
     7741 cggaaaaaaa gtggctggag ttaaaattac caataaggat gtcatcagca aactcagagg
     7801 cgacaaaggc actaaagtaa aagtggatat gtacagacgt ggcgaggcag aacttttaac
     7861 ttttgatatt acacgcgata aaattccatt gtttagtgtt gatgcatcct ataaaataga
     7921 acccgatatc ggttacatta aaataagccg ctttgcagat aataccgtag aggaatttaa
     7981 ggaagcactt gccaagttaa aatcgcaagg agtaaaaagt ctgattcttg atttaacaag
     8041 caacggtggc ggatatctga atcgtgcaca tcagttggcc gatgaatttt taagtgatgg
     8101 gaaacgcatt gtttatacag aaggccgctc acagtccaga gaagattatt tttctactgc
     8161 agcaggtgga tgggaaaaag gcaaacttgt tgtcatgata aatgaatact ctgcatcggc
     8221 aagcgaaatt gtttcgggtg ccatacaaga ttgggacaga ggtttgattg ttggccgcag
     8281 gtcgttcgga aaagggttgg ttcaaaaacc atattcattg cccgatggta gtgccgtaag
     8341 acttaccgtt gcaagatatt atacaccgtc aggacgttgc atacaaaagt catatcagga
     8401 aggcgatgaa gaatacagtg acgacatcag caaccgtttt aagcatggcg aactctatca
     8461 tgccgacagt attaaattca atgactcact taaatacact accaatgcca agcgtgtggt
     8521 ttatggtgga ggaggtatta tgcccgatgt gtttgtacca ttagatacca tgttcagctc
     8581 taaatattat tcagatttaa tccgtaaagc agcattaagt gagtttgcat taacatacgc
     8641 tgacaataac cgcagtaaat taaaatcggc atacacaacg gtagaaaatt ttaaaaagaa
     8701 ttttaatgtt gacgataaaa tgatgaatga ctttattgct tttgcagaaa agaaagaagt
     8761 gaaaaaagac gaagaaggat taaagcgttc aggcactatg attaaagcac agataaaagc
     8821 actcattgcc cgtgatattt ttaatttcgg agcctacttc tacatcataa acgatagtga
     8881 cgatacattt aataaagcag tagaatccat taaggataat acttttagta aaatgaaaat
     8941 tgcaaataat tagatttatt attccttcgt gaatctcagc atttgtattt gatgtaatgc
     9001 attttttatg ctgatgaaat aaatcccttt tgtaaaagtt gttttaattt tttcttttgt
     9061 attacttgtt ataacagaaa gtacaacttt acctactgca tcaaacacag ttaaaacatt
     9121 ttcgccattt gtaagtccag aaattattaa ttcgtcttta acagggttcg gataaataca
     9181 gatgttagca cttccaatgt tatttattga ggttatcaat atatatattg aatcgctaat
     9241 cttggtgcaa ccattgacat cagttaccat tacactataa aatcctgtag cagtgggtat
     9301 gtaacttgta gcggtggcgc caccaattgg tagtcccgat aacaaccatt gattgccgct
     9361 gacagacgaa ctttgaagag tgttggcaat gttggttata aaaggttgtg gtggaattgg
     9421 attaacaata atgttgttta tgatactttg gctacatacc aaattggatg caacaaaggt
     9481 taacgaataa tttccggcac ttaaaccagt gagataaaat gatgaatcat ttgagtatga
     9541 tgtaccatct aaaagccatt cactgttcat atcaaacaat aatgcagagt tggcttgaat
     9601 agagtcgttt tcgcaaatgg tggagttgct aagtgttact gtggccattt ggttgcaatc
     9661 gaaaacagta ttggtaacag tgttggtaat tacgggcgaa ttaaaatcaa aataaatagc
     9721 tgcattattt tgtataactg taccagcagc atttccgggt atttgatcta ttttaaattt
     9781 aatataaccg tggcttgccg gttcgtttga attgctgtct ggtaataaaa tattatcgaa
     9841 atgaaatttt accacattgc ctgaagcaaa ttcataggtg aaagggtgag aagtgttcaa
     9901 tacacttaac gaatatttgt tgagtgataa tggaagcgtg tccgcaatca ttactgcaaa
     9961 agcagtatca gttccggtgt tttggaaacg gatggtgtat ttgagttgtt gtgcggctgt
    10021 aatgtaacct gcagcatcaa aaccatgcgg cattacagct ttatcattag ggtcaaacga
    10081 accggctact gtttgtgaaa tggaatcaat attatttgaa atgatggttt caccaacaac
    10141 tgttgttatc aaagcagaag catttaatac tgttcctaat ggtgtagtag atggaacaac
    10201 agtattaatc tgaaatgatg cagtgccgaa aggaggcagt gatatacctg taaacattaa
    10261 aactccgttg ctgttggttg ccggtgcagg aacagcattt acaaaattta aagtggtgtc
    10321 ataactcacc gttagtgttt gagccggaat tatttttgga gtaagattgc gaacatcaat
    10381 tttgtaatga tgattaaatc cgggtcgagg ttgattgcca ctaatgccaa cttgtaaatc
    10441 gggatttacg tttacactat cggccacttg tacatcaact aatgttccgg gtgtagtaat
    10501 gttaatagta aatgtggtag tatcaggaca tacctgcgca aattggtatg gtgcatattg
    10561 tttaagtgtg taggtgccat ttactaccgg aataaaaaat tcaccggcag cattggttac
    10621 agaactataa ccgccaggat taagttctac caatgttcct gaaaataaaa tatcaccgaa
    10681 accataagaa cagtttgagt tataatcacc aaacacacga ccttttacca aagaacaatt
    10741 aggatcatta acaattgtaa aaggtgtgga ataaatcaaa atagaatcct gtatgttggg
    10801 tatgccttgt gagtagatag tgtcgcctgt aaaaactttt aaagtgtaat ttccgggaag
    10861 aagattcttg taatcgagta atgaatcctg tacagcacga taccctactg tgttgttaac
    10921 agaatctgtt attatggttt tgtatttgga agtaactgca tttccagctc ctgtgtagct
    10981 tgaaattaaa ttgctgttaa aagtattgtt gttaattgca gggtaggggt gaagattaat
    11041 agaaagattt cctgtaggag aaccattgca tgcatccgat acagaaaaag ttggattaat
    11101 agtgggcagt tctttaattc ggaattccac ctgggtgtag catccgaatg gtgccacgcc
    11161 atgcacataa tatttgccag caggtaagct gtcaaaaatg taataaggat ctatggctcc
    11221 gcctacataa taactaaatg tatgtgtcgc gagcaaattt aaagcagtgc tgtcatacaa
    11281 attgtaatta taagtacctt gaggtatgtc attagccgaa ggacctggat aaataactac
    11341 ttgaaatgca ccgttatttg agcccgggtt tgacatagca attgagataa cagaaaaagg
    11401 cacttcaact tgggcattca ccatgccgct gctttgtgct gagtcgcctt gtgcatcaat
    11461 aaccaataaa tcataaatgc cggcaggaac acccaataaa gtatcagtgc cgggaatgcc
    11521 attccaaaag aaggagtatg gtgcaacacc accgtttacc tgagctatta acgcccctga
    11581 gttactgccg caccaggcac ttgtccattg tataacaaac gcagaaatgg gcatttggtt
    11641 attctcagct tttgcctgct gaataatagt aagaaaaaag aaacttaata ttaatttttt
    11701 cattgaataa ataattagtt gaactataag gaattttttc aattacaaaa ttatatagtc
    11761 ataatcgaat aaagaatagt aggaaaaaaa attaaaaaaa attggcttga atatagattg
    11821 tattaattga attaggagtt aagatgaaat tggcaattta taattataga taaatccggt
    11881 tttccaatta attcaaatga gttaatttat ttgaagctat tttattagga aaaataaacg
    11941 gttatagaaa atggcatttc ataagtctat aaacttgcaa aacaaaaata gaatgataaa
    12001 atagcaatat tagcaatgtt attatgctac atgcccggtt ttaaaaaaca actcaacaga
    12061 cgagccattg tttgaaaaaa taatctgatc ggctaaatgt ttcattaaaa agacaccgcg
    12121 tcctgttggc ttttcaagat tttcgggagc agtagggtcg ggcaggttat aatagtcaaa
    12181 tccttttccc tgatcggtaa tagcaaaaga aatagtgtcg ctttctactt cgtagctgat
    12241 tttaactttc aggtttgggt tgtatttgtt tccatgctgt atggcattgt ttacagcctc
    12301 tgttacggct accagaatgt tgccataagc atcttcgtga atgttgtgtt cgcctttaag
    12361 atcgtcaacc aaacgctcta taatatgtat gctttccggt ttagattcta tctgaattgt
    12421 ctggatgttt tttgttgtca tctgtcttaa aattgaagtc cttgaaaata attatttacc
    12481 aaatttttgt aaaatgtatt taaacccgga ggtactgtat tcagtagttc cgtttgtttt
    12541 tcttttagtt ttttatactc ctcaaaagcg gcagggttac gtaaaaattg ctcttttgct
    12601 tgctctgcct tacgtgtctg atcttgttct ctttcacgct gcgcattctc cgcttcaagc
    12661 agtcgtgtaa gtatttcctg ctgccttttt aaggtttctt gcgttatttt tttattcaca
    12721 atatcgcgct cgttttgctc catctgattg gccaaattac cgaggttgcc catagagttt
    12781 ttaccatctt tattttcctg ctggttcaac tggtttaact catttctgat agcctcctgt
    12841 tgggcagcca ttctcgctaa ctcttcgctc atacctttgt cacccttacc cccgtttttt
    12901 tgcattcctt ctaactttcc tttcattttt ttcatttgtt cagaaagttg ttgttgcatc
    12961 atttttaatt tttctgcaga tggtcccggt ttgcctttgc ccggtttctt acatgagcca
    13021 ttgccctgct tctgttgttg ctgctgcatt tgcatttgtt gtaatgcttc actgagcagt
    13081 agtgccagat tattggccga tgtcataatg tattgctgcg agctgcgagc cacaggaacc
    13141 tgcctgtcct gcaaaaatgc aatagatttt tgaatatttg actgaatgtt agaaatttca
    13201 gtatttaccg tctgctgaat ctgaattaca cgcttgctaa gggcaaataa tgagtcttca
    13261 ataattttca ggtcgtcctg cagttttttc tgcctctgac ccatttttac atactgtgga
    13321 ttgttgacat ctgtagtctt taatgcctgc atcagtgctt cctgctcaaa tgaaaaacga
    13381 agcaggttct ctaataaata ccttaatgat gccatgtcct cttccgattg ttccgattca
    13441 ctctgtgcca tttggttttt cattttctgc gacatgttct gcatcttttc cgcagcattt
    13501 ttttgcgatt taccggcatt cgactttttg ttttcgttca actgctgttc gctgttctgt
    13561 tgctcctgat ctatgtcttt ttgctcctgc tctgtgtttg ccagctcctg tggaaattcc
    13621 agttcctgat ttttcttttc taatgctgca atatcttttt taatgtcttc aaactgtttg
    13681 ttcagatcct tctgttgttc ttttaatttt tctgtatcgg aatttttatc catgctttgc
    13741 tccgatagtt ttttctgatc ttctgccagt ttatctaaat tttctaatgt gttttttaac
    13801 ttttgttcta cttctaactg cttaaacaat tccaaagtgc gatccatttg tttttcaaga
    13861 tccttattgt tgagcttcat tttatctaac gcttcttgca cactttgttt gtcgagttgc
    13921 gaaagcattt gctctaattg cttcatcagc tcttttaatt caggattctg caaacggtca
    13981 aacaattcct gtatctgttg ctgtttttcg gctaactgac tttcattgtt cttgaactca
    14041 ttctgcttgg caagattttg ttgttgctca ttttttatct tgtctaactt ttcgtccaac
    14101 tctttttgtt gcttcaacaa atcttcaata cgcttgcggt cttcaaagtc agtttttttc
    14161 ttttctgtca gcttgcgtgc agcatcggta atttgttttt gaatttttga tgcctgttta
    14221 agactttcct gcaagtcttt tttaatttca tcgttacttt tctctgtatt ctctgccaat
    14281 tcacgaagcg taggagcttt aaaaattaac gattgcgatt tagctgattt tggcccattg
    14341 acaccgtcat tgtcccaaac ttcaaaataa tattcaactt catcaccggg tactacacca
    14401 tgcaatgcac catcccaaaa atgataaaaa tctgtctgat tgttattctt gttgattgta
    14461 agaatttctg tatttagttt atcgctatgt gccgatgaat cgtttttact ttttacaaaa
    14521 cgataatgaa aagccaattt cgaaaaacca taatcatcct ttacagtccc tttaaaaaat
    14581 attctttgta atgatgatga atcattttgt tgttctacct gaatgatagg atactgatcg
    14641 ggaattacgt tgatggaata ttctaaagtg tctttattgg tgataaaatt attggcagtg
    14701 gcaatggaat aattagcacc tgaaagcatt cttttgctgt aggtgtattg attgttgtcc
    14761 gacaccaatg gagtgaggct gtcattaaac caaagtttga atactgaggt attaacggtg
    14821 ttgaatgtcc atttcacaat agtgcctgca ggtattaata agtcgcctgt attattcagt
    14881 gtttcgtttt tcttttgaag ataggcagga tagtttaaat caattttgaa tgcagaaatt
    14941 aatggattgg gtactgattt caattcataa ttttcggaat aaaaaccatc agccatcaat
    15001 tgaaaagatg tgctttttat gatgcgatga aaagtaaaag taaaatgttg tttatcttct
    15061 ttgtcggctt taatctgtct tccttcataa gtcacataaa cttgatcggg tagttcctgc
    15121 ccttctactt taaagttaag tgtaaagtca ttctgttcaa ttacattaaa gtttttattg
    15181 agtagaataa aattaaaagg tgcaggtttt ttatacgcta cattgtaatt gacaagtctg
    15241 cttgtacttt ctttaataac gtttggtgct gcaaacagca gaataattaa agtgcctgca
    15301 ggtattaaag ctaatggcaa atattttctg ttctttttaa aatcaatagc accggtaaat
    15361 ggaataggtt tcaggttttg aattttctga tcaatgccgg cctgaaccaa ttcattgtgg
    15421 ttgttttcgc tgagttcatt aagttgaagc gtattgagta gtttatcttt tacatcagta
    15481 aagtggttgc cgataattgt tgcagcttgc tcgtaagaaa gagttttgcc taatttccag
    15541 attttaaaaa gaggaataac tattaatctg atgatgactg caagaactgt aaatacataa
    15601 atatagaaaa gtgctgttcg gagtgaagag cccatccatg caaaatattc tgccaaagaa
    15661 atgaaaagaa aaaatccaat agttattcct gtagcataaa tgctccctct gattacctga
    15721 ttgcggtaat acttacgaat aaactcatcg agtttttcaa tcaatagacg gtaatttcct
    15781 gtcatgtccc tttgttcaat acataacgct aaagtgtggt ttcaggttat gtctgacctt
    15841 aaattttaat aaacatttta cagcctaaaa atagtaaaaa tgagcaggcc aaatgccatg
    15901 catttgcgaa tattgcatga agaggcaaac aaacaaatgt taaaagaaag taagatttaa
    15961 agcagtttaa acgattccag aaatttagtt gtgtaattgc cttttcggaa gtcttcgttt
    16021 ttcatcaact gaatgtgaaa agggatggta gtttttacac cttcaatcac atattcactt
    16081 aatgcacgct ccatagtatt tattgcttct tctctggttt gtgcaatagt gatgagtttg
    16141 gcaatcatac tgtcgtagtt tgaaggaata gtatagcctg cataaacatg cgagtcaaca
    16201 cgcacaccgt gtccgcccgg aacgtgcaga ttggtaattc tgcccggtga tggtctgaag
    16261 ttgttgtatg gatcttctgc attgatacga cattcaatgg catgcatggt agggtagtag
    16321 tttttacctg atataggttc gcctgatgca atttttattt gctctttaat aaggtcgtag
    16381 ttgattactt cttccgttac agggtgttca acctgaatac gggtattcat ttccatgaag
    16441 tagaaattgc ggtgtttatc cacaagaaat tcaactgtac caacaccttc gtattttaca
    16501 gctaaggcag ctttaattgc tgcttcaccc attttttcgc gcaattcttt tgtcatgaaa
    16561 ggcgagggtg tttcttcaac aagtttctga tgtcgtcttt gtaccgagca atcacgttcg
    16621 gagaggtgac atgcttttcc atattgatca cctgcaatct gtatttctat atgtcgtggt
    16681 tcttcaatat atttttcgag atacatacca tcgtttccaa aggcagcgcc tgcttcctga
    16741 cgtgcactgt cccatgccgc tgcaaactct tcgtctttcc acactatgcg cattcctctt
    16801 ccaccaccac cggcagttgc tttcaggatg acaggatact ttatttcagc agcaattttt
    16861 aaaccttctt caacactggt aagtaatcct tcagagccgg gaatagttgg tacaccggcc
    16921 tttttcatag tgtcttttgc attgctttta tcgcccatgc catcaatctg ttcaggcgtg
    16981 gctccaatga attttatttt attcttggca caaatagcgc tgaactttga attctctgat
    17041 aaaaatccat atccgggatg gatagcatca gaattggtga tttctgctgc tgcaataata
    17101 ttggggatat taagataaga atctttgctg ggtggaggac ctatacaaac ggcttcatca
    17161 gcaaagcgaa catgcagact ttcgcgatcg gcagtagaat agactgccac cgttctaata
    17221 tccatttctt tacaggtgcg gatgatgcgt aaagcaattt cacctctgtt tgcaattaat
    17281 atttttttaa acataattag tttggtaatg atgaaattaa aaaagcgcca aggagcttat
    17341 aattctaaga aaaatattgt tgaatgaatc aggatggatc aattaaaaac aacggttggt
    17401 cgtattctac aggagtggaa tgttctacca aaacttttac aattttacct ttaatgtcac
    17461 tttcaatttc attaaacagc ttcattgctt caataataca gattacctga ccttctttta
    17521 cttcgtcacc aacatttaca aaatttggtt tgtcgggcga tggagttctg taaaaggtgc
    17581 caatcattgg cgacttgatg gttttgtatt ttgaatcatc acctgcagtt tttgcggcag
    17641 gagcggctgc aactgcagga gcagcaatta cttgttgggg ttgagcaggt gccggcattt
    17701 gcatgactgg ctgaactgct ggtgctacat gtacaatttg ctctttggtt gggtttttaa
    17761 tggttatttt aaaatccttt tgttcaaggt gcacttcact cactccggct ttggctacaa
    17821 agcgaattaa ttcttcaatt tcttttttgt tcatatactg ttgtttttta caagtctttt
    17881 cgtgaaaatg aatcaattat aacataaccg ctacaatagg tcatttcaaa agacagcatc
    17941 aaaagtagaa actttttaca aatatgatga aatgggcaaa aaagaaaaaa gggcagcctt
    18001 atgggctgcc ctcgcttatt ttggtttcag cgctgccggt ttactctata accagcttat
    18061 ttctttgata atttagctca atcatataca ctcctttagc aaattgagca aggctaatct
    18121 gaactgactg acgaccggaa atggtttgaa ttttttgcac cataacagtt cgtccgctca
    18181 aatcaagaac tttaagatct ttttgcatgc catctttatc agaccagaat tcaatgttta
    18241 ctacatctga tgcaggattt ggatacatat tgaagttagc tgcagtcaac tcttccgatt
    18301 cgcgacaatt ggttgttgca ttataagttt tagttgcaga accacatgca ttacttgctg
    18361 ttattgcaat tacaccattg cctgtacccc agtcaagcgt aagactgttt gaattacccc
    18421 caccggcaac ataagttgca acaggtgcag aaggatagcc ccagtcaagt gtataagaac
    18481 cggttgtatt gctgatatta gctgtcaatt caataccttg tgtatttgcg caccagcttg
    18541 aaggtaaagc tattatgtca gttggaggat ttggagctcc ttttacagct acacaacgtg
    18601 gagcacttgt gccgcatgtg ttacttgcaa caacacaaac ctgtcccgtt gtcatagtag
    18661 aaaactgaat gttaacagag ttgtttcctt gtccgaatac attagcgcct gcaggtgctg
    18721 tccagttgta gcttgtacct gtttgtatag gtgtactgta agctacagat tgattacaca
    18781 gtccatttgt aatacctgta attgcaggag gcaccattgg tattccggga gccacagttt
    18841 tgcattttaa tgcagatgta acgccacaaa tacttgtaac acttacacaa acatttccaa
    18901 tattattgta accatttcca aaggtaacat ttattgagtt gttgtttgat gaccctgaag
    18961 caccagttgg taaagtccag ttataaactg ctgcgccttg taaaggtgtt acagagtaag
    19021 tctgacttgt atttggacat gcagtgaaat tacctgaaag attatttggt attccggcag
    19081 atttgctaag actaatacat tttgatgcac tggtataaca tgatgtttgt gccgctacac
    19141 aaagtgttcc gccattccaa gtcggaccaa agtcaatatt tacattggtt ccattgcctg
    19201 taaccgttgc atcaccggta atactccact gatattggct tgctccagta attggtgcaa
    19261 tgttgtaagc aacattactt gtgttttcac aaacagtttg tgggccaagc actgtggcag
    19321 gcacagaaac aatacctctt actttttgaa tttttcttaa ggtacttcca catgcatttg
    19381 ctgcctgaac acctacatta taaaatgaac cattcggtga tccgaaagtc acttgtacgt
    19441 taggtgaact tgttgtataa ggtgatacat ttaaagggtt gccatcaaaa taactaccta
    19501 taggtgcatc ccaaacatat tgtgtagtat tattaacagc attaatcgaa aggtttgcag
    19561 tagttccaac acaagcattt aatggcatcc ctgtcattgg cacagtaact gctgcagaag
    19621 gaacaccaac tgtaatagct aatgataaat cagcacatgg tgtggcacca caaacatctt
    19681 ctgcacgtac ataataagat gtacttgatg atggatttac tgtaattgat ggtcctgaac
    19741 caacaggcgt tgtaccacaa tttgttttat accatttcca atcagcagta gatcctaaag
    19801 aaccacctac agcagatagt gttgttgaac cacctataca aagttctgtt ccgttttgtg
    19861 atgaaacaga caatgcagca gttgatggtg tatttacaag aattaaatct tgttgctgag
    19921 tgcttgttgc acacatgtga gcatcttgta ccattacatt ataacttccg gcagttaagt
    19981 tgagatttgc attgttgttt acataagtac tgccgttgtc ataactatac atgtatggtg
    20041 ttgtaccacc tgttgcattc attgttatgc taccaaatgt tgatggacat tgctgatctg
    20101 tatatgtttt ggtaaactgc agcaatgccg gctccgttat tgtagctgta actgtattct
    20161 ggcaagcatt ggcatcacta acatagattg tataggaacc ggcaggaagt aaatcaaact
    20221 cacctgttgt attaaagttt acgaagtcgt aagtatagga atatggtcct gtaccatttg
    20281 aagtgttggt agttattgat ccattgctct caccaaaaca atttacgtta atctgtgtgg
    20341 aaacatttat tacagggttc ggatttacat caacctgaac agttgttgtt gcagtacata
    20401 gatattgatt tgttacaaca acagtatatg ttccgttata tgcagaagta gcactaaaaa
    20461 tattagggtt ctgctgagaa ctgaaaggaa gattgtttgg atcataccac tgatagctat
    20521 ttccgcttcc ttgtcccccg gctaagttat ctgcaataaa gttaatatca tttccatcac
    20581 agacaggcgc attactgctt gctattgcca aaggtcccgg tgttgcactt acaccaacag
    20641 ttgtgtttgc ggtgcaacca ttagctgttg cagcaagatt atatgttgtt gttcctgtta
    20701 cagtagcaga aggattttca tctgttgaat tgaatccgtt aggtgttgat gtccaagtat
    20761 aagtaatacc tgaagaggtc ataatggtaa ctgtccatga aacaattgtt cctacatcgc
    20821 cactggctct gtcagacatg ttaagtaccc agtttcctcc ggcattcatt gggaatgaga
    20881 ttccgctcca atttgtattt attgaaccat ttgtatttga aggtgcataa gttccggcag
    20941 gaatcgttgt agggcccgag tttgattctg gaagaactga acctgcactt acatcaaact
    21001 cataatagcc accaaaattc tgaccatttc caaaacttga agcaggtaca ccaagacggt
    21061 caaacacagt tattactcct cctggtgaag ttacttttgc aataacatca cctgaatagg
    21121 tatgagtaag attaatacca attttcag
//
LOCUS       DAOOOI010000041        21008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_493_length_21008_cov_49.312223, whole genome shotgun sequence.
ACCESSION   DAOOOI010000041 DAOOOI010000000
VERSION     DAOOOI010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 21008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21008
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_493_length_21008_cov_49.312223"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            340..3204
                     /gene="gcvP"
                     /locus_tag="PKX59_06585"
     CDS             340..3204
                     /gene="gcvP"
                     /locus_tag="PKX59_06585"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOZ90570.1"
                     /translation="MYYSFADRHIGPDDKQVKHMLATIGVKSVNDLIAKIIPELIRLQ
                     KPLNLPEAMSEFEYLNELKATASENQVFKNFIGMGYYNTIVPGVIKRNIFENPGWYTA
                     YTPYQAEIAQGRMEALLNFQTMVCDLTGMQIANASLLDEGTAAAEAMVMFYYTRSKEA
                     EKKNACKFFVSENCFPQTIDVLRTRSKPLDIELVVGDEQLFSPDDSFFGALLQYPDAI
                     GNVLDYKNIVNMCHRHEVRVCVAADILSLVLLTPPGEWGADCVVGSTQRFGVPMGYGG
                     PHAAYFATHDVYKRNLPGRIIGMSVDAEGNPALRMALQTREQHIRRDKATSNICTAQV
                     LLAVMASMYAVYHGAEGLKAIATTVHKRTTALAESLKKAGFKVVNNSWFDTLTVEVED
                     TESVRDMAEANLINFRYNGNTVGVSIDETTSDEDLKDILRVFAASIGKKSIAAATMNG
                     NAIPAALLRSSEYMTNAVFNTHHSETEMLRYIKRLENKDLALNHSMISLGSCTMKLNA
                     TSEMEPLSWPEFANMHPFAPIEQAQGYAHIISELENFLKEITGFAAVSLQPNSGAQGE
                     YAGLVVIQKYHSDRGQSHRNVVLIPQSAHGTNPASAVLAGLKVVVVKCDDDGNIDLSD
                     LRLKAEQHQENLSCLMVTYPSTHGVYEENIKQITAAIHANGGQVYMDGANMNAQVGLT
                     CPALIGADVCHLNLHKTFAIPHGGGGPGMGPIGVAEHLAPFLPSHTVVPTGGRKGVNA
                     VSAAPWGSALILLISYGYIKMLGKTGVQRATEIAMLNANYIKARLEKHYPVLYKGKNG
                     FCAHEMILDCRAFKAVANVDVTDIAKRLMDYGFHAPTVAFPVAGTLMVEPTESEPLEE
                     IDRFCDAMIAIRHEIMEVINGEYSKEDNMLVNAPHTALEATADEWKHSYSRQKAVFPA
                     PWVAGNKFWPSVKRVDNAYGDRNLICTCPDVSAYETAEAV"
     gene            complement(3218..3640)
                     /locus_tag="PKX59_06590"
     CDS             complement(3218..3640)
                     /locus_tag="PKX59_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90571.1"
                     /translation="MDAAIENFISAQKNMTLCTSVNDIPECANCFYVFMQDGAHLIFK
                     SKKTTGHVSHALVNNKVAGTILPDISKIGNIKGIQFSGIFLEPQDVMLEQAKKIYYSK
                     FPLALGISGDLWLIKLTYIKMTDNTLGFGKKLIWENKN"
     gene            complement(3641..3868)
                     /locus_tag="PKX59_06595"
     CDS             complement(3641..3868)
                     /locus_tag="PKX59_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOZ90572.1"
                     /translation="MIEIVQYRSKCIGCNACVEASATRWRMSRKDGKCTLVGGKNNKG
                     IYIAVVNDDEYEQALQAAKNCPVNIIKVKKR"
     gene            complement(3861..5087)
                     /locus_tag="PKX59_06600"
     CDS             complement(3861..5087)
                     /locus_tag="PKX59_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOZ90573.1"
                     /translation="MTEIMSPAGSFESLQAAIQGGADSVYFGIEQLNMRARAANNFTL
                     SDLAEIKKICSEKNVKTYLTLNTVMYDHDIMLMKKIIDAAKKNNIDAVIASDHAALMH
                     AKKIGMPIHISTQANVSNIESVRFFADYADTIVLARELTLNQVADVVKQIKRQKIKGP
                     SGKLIKIEVFAHGALCMAVSGKCYLSLHSHNASANRGACIQNCRREYIVQDKENEVEL
                     EIDNEYIMSSKDLCTIDFIDKLIETGISILKIEGRGRAADYVYTTTKCYKEAVECYHN
                     GSYSATKIEHWKNELATVFNRGFWGGYFLGKTMGEWSNTGGSQATTKKIYVGKGVKYF
                     PKNKVGEFKLESGTLSVDNDIMITGSTTGIILTKVQELRMDDLQVTTVKKGDVFSMPV
                     AEKIKPSDKLYKIVND"
     gene            5276..6325
                     /locus_tag="PKX59_06605"
     CDS             5276..6325
                     /locus_tag="PKX59_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90574.1"
                     /translation="MNKTFTLITTLFCAAAINTNAQLITISATHQMPAIGDTIRYVDA
                     NTFGFNQNGTGPVTNTLWDNALLMNAGTNYSFFYDDPATITGNGVDSFPGATLARGES
                     GAAGYFYYQNTANDINRIGWYSSASNYGVYNTGTYATEFHFPMTAGQTVNSTYSGRYA
                     PFGLGEDSCTLESGSLAINADQQGTLILPTGTFTNVLRIHAIETFHIKIYMLGTPVID
                     YLVSDDYYYWFHDTIKQPLLTYGITTIDGTAQTPVLRYQPVSGTTGLNQLAAHTEMTI
                     TPNPSSGKFLLSNVGSTNATIEVLNALGQKVNFQQTNMGQSLEVDLNNQAEGIYFIKV
                     TDGRTSTTHKVVVGF"
     gene            6605..8455
                     /locus_tag="PKX59_06610"
     CDS             6605..8455
                     /locus_tag="PKX59_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ90575.1"
                     /translation="MLFFIVFTAYSQDERDSISTHTVKEIIIQGNRLETPFSEAARDI
                     QIITQKEIAALPVQTLTELLVYVSGVDMRQRAPFGTQGDISIDGGSFEQTLVLLNGIK
                     LINAQTAHNVLNLPISLDAIDHIEVLRGAAARVYGINALTGAVNIVTKKSNESFVTAN
                     IYGGTSFKRKETGDGDGMYGGGGVDLVGNFANNNHRQLLGLSQNLFNGYRYNSAHKNT
                     KIFYDGDFYINEKNSISTFAGHTYNKFGANGFYAAPGDKNSEEIVETSIFSLSSKHQL
                     GKVNLMPRISDRYDEDDYRYFKSDLSRGRSKHFTNALMLELNGNLTTSAGVIGMGWES
                     RLTKINSSNIGEHKMDNHGAFIEFRTKLFKRLVTNVGIYVNYNSQFDWKAYPGLDLAY
                     RLNNKWKIAASFGSGQRIPSFTDLYLKQPPANVGNPDVQPETAWQYDANIEFTSGHLK
                     VNVGSFYRKVTDFIDWVRPNMTTPYSPSNFGNIIVQGIYSRINQQLILSGKHQLNFCI
                     SYNFLNPEHYDYKNEQSKYVLESLRHQLIAGVNYRYGSFNVQIENRYIKRELNDAYNI
                     VDGRISYEIHSVLFYANVSNMFDAKYKEAGAVPMPGKWFALGLKYNWKKK"
     gene            complement(8497..9873)
                     /locus_tag="PKX59_06615"
     CDS             complement(8497..9873)
                     /locus_tag="PKX59_06615"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOZ90576.1"
                     /translation="MNTIVTLSIVGIAAMLSEVFKLKRIMYTLVIAGLVVALGFSIND
                     WNNFGSYFNQMVMVDSFSIVFSSLMIVTTLLWMIMSRSYFSSDSSMSEHFALIVFALT
                     GGVMMTTFSNMSMLFLGLEILSFALYIMAGSDKTNIKSNEAAMKYFIMGSFATGFLLF
                     GITLIYGATSSFNIQEIANYTQQNKDALPAFFKVGVLMILIGLSFKIAAAPFHFWTPD
                     VYDGAPTLVTAFMASVVKTAAFAMIYRLFVSCFISIYDFWTPVFAVLSAITMLAGNIT
                     AVYQTSLKRMLAYSSVAHAGYMLMALAAPGIASKNALLFYLAAYSLSALGAFNLLYSV
                     HKVTGNEGIKAVKGLFYKHPVSAIMLTICMLSMAGIPPVAGFFGKYYLFTAALKENLL
                     WLVLIAILSSLIGIYFYFKVIIAAFQQPETENTGYCERPSDLAVLGICAVLSLILGIA
                     PQLISGLL"
     gene            complement(9899..11356)
                     /locus_tag="PKX59_06620"
     CDS             complement(9899..11356)
                     /locus_tag="PKX59_06620"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017256772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOZ90577.1"
                     /translation="MNNLLLIILFLPLAGMALSLLPSREATGKTAFVFSLISLLLSVC
                     LFIQFDPNGGMQYVINYAWVPDLGINFKIGVDGISLMMILLTNVLTPLIILSSFGRRI
                     ENPKGLYALIQLMQFALVGVFISLDAFLFYIFWELALLPIYFICLLWGGKDRIRITLK
                     FFIYTITGSLLMLVGLIYLYLQTPGSHTFDIQAFYDLTLSSQQQTLVFWLIFVAFAIK
                     IPVFPLHSWQPDTYTDAPTQGTMLLSGIMLKMGLYGVLRWLLPIVPGASADNAFIVML
                     LGIIGVVYASCIAIVQKDFKRMIAYASIAHVGLITAGLFTLKVEALEGAVFQMLSHGI
                     NAVGLFFIADMLMSRLKTHNMSEMGGIANNNSLFAVLFMIVLLGTVALPLTNGFVGEF
                     LLLTGIYQFKAWMAIFGGLTIILGAVYMFRSYQAIMLGSRHLNEGEFHPMTLSEKTVL
                     GTIAFLVILLGVYPSFLMNAAAPSIKSLLMIINAG"
     gene            complement(11357..13258)
                     /gene="nuoL"
                     /locus_tag="PKX59_06625"
     CDS             complement(11357..13258)
                     /gene="nuoL"
                     /locus_tag="PKX59_06625"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOZ90578.1"
                     /translation="MDFLISAIPLVPLIGFLLNGIINKKMPQSLVGIIASGTVLFSFL
                     ISCYLFLQINNGSIKESYYSMFSWINAGSLNLGLSFLVDRLSVIMLLVVTGVGFLIHV
                     YSIGYMKHDEDYSRFMSYLNLFIFFMLLLVMGSNYVVMFAGWEGVGLCSYLLIGFWFK
                     NNDYNKAANKAFIMNRIGDLGFLLGLFLMYQTFSSLEFKDVFAAAGNYPSGDATLTAI
                     ALFLFIGAVGKSAQIPLYTWLPDAMAGPTPVSALIHAATMVTAGVYMVIRSNVIYTLA
                     PFAGEIVAVIGLVTALMAASIGLKQNDIKKVLAYSTVSQLGYMFVALGVGAYTTALFH
                     VVTHAFFKALLFLCAGSVIHAMSGEQDIRRMGGLKKYLPITHFTFLMGVLAISGVPLF
                     SGFFSKDEILAVTWTYSPVMFTILAITSVLTATYMFRLYWLTFYGDFRGTHHQKEHLH
                     ESPSSMTVPLIILAVFSVIGGYIGMPEYINEKLGTHHVFTQFLNPIIYSPKEFILSHT
                     FEFTLLIGTIIGLAIIYVMTKNMYVRDKVVPEDDSQTKSFQKVLANKYYVDELYDMII
                     RKPLEAFGELFYKIFDISIIDGIVNFIPRATNLTSSGLRLLQNGSVGFYLVSMVIGII
                     LIFVFKMLL"
     gene            complement(13262..13570)
                     /gene="nuoK"
                     /locus_tag="PKX59_06630"
     CDS             complement(13262..13570)
                     /gene="nuoK"
                     /locus_tag="PKX59_06630"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOZ90579.1"
                     /translation="MEPVNINHYLLLSAILFCIGVAGVLFRRNAIIIFMCIELMLNSV
                     NLLLTAFSNQHNDASGQVFVIFIMAVAAAEVAVGLAILVMIYRNVKSTDIDVLNKLKW
                     "
     gene            complement(13572..14027)
                     /locus_tag="PKX59_06635"
     CDS             complement(13572..14027)
                     /locus_tag="PKX59_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOZ90580.1"
                     /translation="MSALFTVFSKKPVYSVLYLIFFFFTLFGHYILLNAQFLAAVHLI
                     VYAGAIMVLFLFVIMMLNLNKEIEPNKNIVSKFAAVISSGLLLIVIVSVLKHVDVESS
                     KLNSQTDIGLVESIGHVLYNEYMLPFEMVSILLLTALIGAVMLGKKEVK"
     gene            complement(14133..14396)
                     /locus_tag="PKX59_06640"
     CDS             complement(14133..14396)
                     /locus_tag="PKX59_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90581.1"
                     /translation="MSCDKDVCRDCYLVRSMLCTYTDTTLAAYIIPGDTVKTEQQCDK
                     EKDFKDDKFKEEKYEYDSPSMFYPNYNVHVVVTDIYHCPEKTN"
     gene            complement(14453..14998)
                     /locus_tag="PKX59_06645"
     CDS             complement(14453..14998)
                     /locus_tag="PKX59_06645"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOZ90582.1"
                     /translation="MMLTNRSKVVVNKEMTFAEKIYIPAIVAGLMITIKHFFKKKATI
                     NFPEQQKPISEVWRGLHVLKRDEQGRENCTACGLCAVACPAEAITMTAAERKKGEEHL
                     YREEKYAAVYEINMLRCIFCGLCEEACPKEAIFLTNRMTPSDTLRDNFIYGKDKLVEG
                     IAPEERVDISYRARAHRKEVE"
     gene            complement(15040..15255)
                     /locus_tag="PKX59_06650"
     CDS             complement(15040..15255)
                     /locus_tag="PKX59_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90583.1"
                     /translation="MKSSKFKNYKNSNLFKEALEFAAIGNRGVQLAMIDSKKRKIPTV
                     FGIMDMVFYRLPDGSITTKSPFKKRKK"
     gene            complement(15252..15821)
                     /locus_tag="PKX59_06655"
     CDS             complement(15252..15821)
                     /locus_tag="PKX59_06655"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ90584.1"
                     /translation="MTKELLIVGGPNGSGKTTFAKSFLQEYKYEFLNADEIARELSND
                     GSRAGNISSGKEYFKRIEKLKAKNKNIILESTLSGLFLRKLIREFKKKDYSIKIVFIV
                     LNAPEQCIGRIKHRVETGGHFVPDDDVRRRFTRGKNNFWKIYKDLVEDWKLFNNTESG
                     FEIIAAGEKNKYDIVNQSLFDLFIKDIEK"
     gene            complement(15836..16876)
                     /gene="nuoH"
                     /locus_tag="PKX59_06660"
     CDS             complement(15836..16876)
                     /gene="nuoH"
                     /locus_tag="PKX59_06660"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOZ90585.1"
                     /translation="MEVSVLIFKTVFVIAVFLVSLLIAMYSTYAERKVAAFLQDRVGP
                     NRAGPFGILQPLADGVKMFMKEEIIPSHADKALFILGPSLMMLTACMTGVVIPWGDTL
                     IINGKEYFMHITDVNIGVLYIFGVVSIGVYGIMIGGWASNNKYSLLGSVRASSQMISY
                     ELAMGMSIIALIMVTGTLSVREIVVQQQGFHWNVFYQPLGFLIFLICAFAECNRTPFD
                     LPECETELVGGYHTEYSSMKLGFYLFAEYINMFISSAVISALYFGGFNMPFISELGLS
                     HNALTLLGVLFYFIKIFFFIFFFMWVRWTIPRFRYDQLMNLGWKMLIPLSILNVLLTG
                     VFLLWKNGLFHF"
     gene            complement(16886..17860)
                     /locus_tag="PKX59_06665"
     CDS             complement(16886..17860)
                     /locus_tag="PKX59_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOZ90586.1"
                     /translation="MPKVTIDGHTIEVPEGTTILEAARKIGGDIVPPTMCYYSKLKTS
                     GGYCRTCLVKVSKGSEKDPRPMPKPVASCRQTVMDGMEVQNITSPEILDARAGVVEFL
                     LINHPLDCPVCDQAGECNLQDLSYEHGHEVSRLDFRKRTFPMIDIGDKIKLHMNRCIM
                     CYRCVKVAEQLCPERVHGVLNRGWDSEISTYIEQAVDNEWSGNMIDVCPVGALTDKTF
                     RFKSRVWFTKPVDAHRDCPTCSGKVVLWYQGEEVLRVTGRKDQWGEVDEFICNECRFD
                     KKKTSDWVIEGPRHIDRHSVISQNHYEELKKLKQDLSRQKKLGEKAHN"
     gene            complement(17860..19197)
                     /gene="nuoF"
                     /locus_tag="PKX59_06670"
     CDS             complement(17860..19197)
                     /gene="nuoF"
                     /locus_tag="PKX59_06670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOZ90587.1"
                     /translation="MSRKLLLDNLDKPGIQSITGYSDNGGYEALRKALQMKPEEIVSE
                     VQKSGLRGRGGAGFPTGMKWSFIAKPEGVPRYLVCNADESEPGTFKDRYLMEHKPHIL
                     IEGMIISSFALGANTSYIYIRGEMMYVFHILQKAIDEAYAAGYLGKNILGSGFNLDLH
                     VHCGAGAYICGEETALIESLEGKRGNPRIKPPFPAVKGLWGCPTVVNNVETIAAVVPI
                     IKITGEEYAKIGIGKSTGTKLISASGHINKPGVYEIELGVPVEEFIYSDAYCGGIKNG
                     KQLKAVVAGGSSVPVLPAHLILKTAAGEPRLMTYESLADGGFATGTMLGSGGFIVMDE
                     DTSVVKNLWTFAHFYHHESCGQCSPCREGTGWMYRILNKILNGKGEMKDIDLLWNIQG
                     NIEGKTICPLGDAAAWPVASSIRHFRSEFEAYINNHGMKDVKHYYRLNNLQEA"
     gene            complement(19208..19750)
                     /gene="nuoE"
                     /locus_tag="PKX59_06675"
     CDS             complement(19208..19750)
                     /gene="nuoE"
                     /locus_tag="PKX59_06675"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOZ90588.1"
                     /translation="MSTNKNIQFSDDAMALVQKIINRYPEGKQKSALLPILHIAQAEF
                     DGWLSPEVMDYVASLLKIQSIEVYEVATFYSMFNMKPVGKCLIEVCRTGPCWLMGAED
                     VVRYIEKKLGIKAGETTADGMFTLKTVECLASCGTAPMMQIGETYHENLTLEKVDDIL
                     NDHKNQGNRRTHWEKDKVKP"
     gene            complement(19765..20973)
                     /locus_tag="PKX59_06680"
     CDS             complement(19765..20973)
                     /locus_tag="PKX59_06680"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOZ90589.1"
                     /translation="MKEEIKQELIGREYTTLNLGPTHPATHGIFQNVLKMDGEIIIES
                     TPTIGYIHRAFEKLAERRPYGQVTTITDRLNYCSSPINNMGWHMTVEKLAGIRLPKRV
                     EYLRVIIMELARIADHIICNSVIGVDSGAMTGFLYVFQFREHIYEIYEEICGARITTN
                     IGRIGGLERDFSQTAWLKIEKLLKELPKGLKEFEDLLIRNRIFMDRTINCGPISAEMA
                     LAYSFTGPNLRAAGIDYDVRVMNPYSSYEEFDFMIPLGTNGDTYDRFMVREQEMKESM
                     SIIKQAMQKLDKLEDKTSFNAEVPEFYLPPKNEVYNEMEALIWHFKIIMAETAVPKGE
                     VYHCVEGANGELGYFLISDGGRTPYRLHIRRPCFIYYQAYPEMIKGAYLSDAILTMSS
                     MNVIAGELDA"
BASE COUNT         6623 a         4037 c         3771 g         6577 t
ORIGIN      
        1 atgtaatata tagcatttgt taaaaatttg ataatgtggt gttatataga taatcgaatc
       61 ggaggaggaa agtttttatt ttggagatgg ttttttgcat gagggatagt agcgaaaagc
      121 ccgcatgctg ccgcaggcag gagcggactt gcagcgtata gcccgacagc gccacaggcg
      181 gtgacatgcc caagttttag aaatattgaa atatcaacat gtgttaataa tttttgtgct
      241 aaaaagcggt aaatgcttga tgtaaatcat gttgaatgct ttcagaaata cattttttaa
      301 cgttctaatt atttactttt gcggcaaaat ccagagtaca tgtactattc ttttgccgac
      361 aggcacattg gccctgatga caagcaggtt aaacacatgc ttgccaccat tggcgtaaaa
      421 tccgtaaatg atcttattgc taaaattatt cctgaactga ttcgtttgca aaaaccatta
      481 aaccttccgg aggcaatgag cgagtttgaa tatttaaatg aattgaaagc aacagcttcg
      541 gaaaatcagg tttttaaaaa ttttattggc atggggtatt acaataccat tgtgccgggt
      601 gtaataaaac gaaacatttt tgaaaatccg ggttggtaca cagcatatac tccttatcag
      661 gcagagattg cgcagggcag gatggaagca ttgctgaatt ttcagactat ggtgtgcgat
      721 ttgacaggta tgcagattgc aaatgcatcg ctgctggatg aaggcactgc tgctgcagaa
      781 gcaatggtga tgttttatta tacacgctct aaagaggcag agaaaaaaaa tgcatgtaag
      841 ttttttgttt ctgaaaactg ctttccgcaa actatagatg tgttgcgcac acgcagcaaa
      901 ccattggata ttgaattggt tgttggcgat gagcaattgt tttcgcctga tgacagtttc
      961 tttggtgcat tacttcagta tcctgatgca ataggtaatg tgttggatta taaaaacatt
     1021 gttaacatgt gtcaccgaca tgaagttcgt gtttgtgtag cagcagatat tttaagtcta
     1081 gtattgttaa cgcctcccgg tgaatggggt gccgattgtg tggtaggatc aacacaacgt
     1141 tttggtgtac ctatgggata tggcggtccg catgctgcat attttgcaac acatgatgta
     1201 tataaaagaa atttgcccgg aagaattatc ggtatgagtg tagatgcaga aggcaatcct
     1261 gctttacgta tggcattgca gacacgcgag caacatatca gacgagataa ggcaacaagt
     1321 aatatttgta ctgcgcaggt gctgttagca gtgatggcat caatgtatgc agtgtatcat
     1381 ggagcagaag gtttgaaggc tattgctact acagtacata agcgtactac tgcacttgca
     1441 gagagtttaa agaaagccgg atttaaagta gtgaacaact catggttcga tacattgact
     1501 gttgaagtag aagatacaga gagtgttcgc gatatggctg aagctaatct gataaacttc
     1561 cgttacaatg gaaatacagt gggtgttagt atagatgaaa caacctctga cgaagatttg
     1621 aaagatattt tgcgtgtgtt tgctgcaagt attggtaaaa agtctattgc tgctgcaact
     1681 atgaatggta atgccatacc tgctgcgcta ttacgcagtt cggaatatat gaccaatgca
     1741 gtatttaata cacatcacag cgaaacagaa atgttgcgct acatcaaacg tctcgaaaat
     1801 aaagatcttg cattgaatca ttcgatgata tctttaggtt cgtgcaccat gaaactaaat
     1861 gccaccagtg agatggaacc actaagctgg cctgagtttg ccaacatgca tccatttgct
     1921 cctatagaac aagcacaagg ttatgcacat attatttctg aactggaaaa ttttctgaaa
     1981 gagataacag gatttgcagc ggtgtcatta caacctaaca gcggtgcaca aggagaatat
     2041 gccggtttag ttgttattca gaaatatcat agcgacagag gacagagtca tcgcaatgtg
     2101 gtattgatac cacaatctgc acatggaacc aatcctgcca gtgctgtact tgccggatta
     2161 aaagttgttg ttgttaaatg tgatgatgat ggaaacattg acctgagcga tttaagactt
     2221 aaagcagaac agcatcagga aaatttatct tgtttgatgg tgacatatcc ttcaacacat
     2281 ggagtgtatg aggaaaatat taaacagatc acagcagcaa tacatgccaa cggtggtcag
     2341 gtttatatgg atggtgcaaa catgaatgca caggtaggtt taacatgtcc tgccttgata
     2401 ggtgctgatg tttgccattt gaatttgcat aaaacatttg ccataccaca tggtggaggt
     2461 ggccccggaa tgggacctat tggtgtagct gaacatcttg cgccattttt accttcgcat
     2521 actgttgtcc cgacaggtgg acgtaagggt gtaaatgccg tgtctgcagc gccatggggc
     2581 agtgcattga ttttgttaat cagctatggt tatattaaaa tgcttggtaa gacaggtgtg
     2641 caacgtgcaa cagaaatagc aatgctcaat gccaattata ttaaagcacg attagaaaaa
     2701 cattatcctg ttttatataa aggcaaaaat ggtttttgtg ctcatgaaat gattttagat
     2761 tgtcgtgcat ttaaagctgt tgccaatgtt gatgtaactg atattgccaa gcgactgatg
     2821 gattatggtt ttcatgcacc tacagtagca tttcctgttg caggaacgct tatggtggaa
     2881 cctacagaga gtgagccatt agaagagata gatcgttttt gtgatgcaat gattgctatc
     2941 agacatgaaa ttatggaagt aatcaatggt gagtacagca aagaagataa tatgcttgtg
     3001 aatgcgccac atactgcact tgaagcaaca gcagatgaat ggaagcatag ttactcacgt
     3061 cagaaagcag tatttccggc accatgggtt gcaggaaata aattctggcc atcggtgaaa
     3121 agagttgata atgcttatgg tgacagaaac ctgatatgca cttgccctga tgtttcggct
     3181 tacgaaaccg cagaagcagt ttaattgatt ttttatatca gttcttgttt tcccatatca
     3241 gcttttttcc aaagccaagc gtgttatcag tcattttgat gtaggttaat tttatcagcc
     3301 ataagtcgcc cgatattcct aatgccagag gaaatttact gtaataaatt ttcttcgcct
     3361 gctccagcat gacatcttgt ggttcaagaa aaattcctga aaactgaatg cccttgatgt
     3421 ttccaatttt acttatgtct ggtaatattg tgcctgccac tttattattt acaagtgcat
     3481 gagaaacatg accggtagtt tttttggatt taaatatcag atgagcaccg tcctgcatga
     3541 atacataaaa acaatttgca cattcgggta tgtcgttaac cgaagtgcat aatgtcatat
     3601 ttttttgcgc actgataaaa ttttctattg ctgcatccat ttatctcttt tttactttaa
     3661 taatatttac cggacaattt tttgctgcct gcagtgcttg ttcatattca tcatcgttta
     3721 caacagcaat gtagatgccc ttattatttt ttccacctac aagagtacat tttccgtctt
     3781 ttcgtgacat acgccatcgt gttgccgagg cttcaacaca ggcattgcat ccaatacatt
     3841 tactcctgta ttgtactatt tcaatcatta acgattttat aaagtttgtc tgatggtttt
     3901 attttctcag caactggcat tgaaaacaca tcaccttttt ttacagttgt aacctgtaaa
     3961 tcatccattc gcaattcctg aaccttggta agaataattc ctgttgttga accggtaatc
     4021 ataatgtcat tgtcaacgga tagtgttcct gattccagct taaattcgcc tactttgttt
     4081 tttggaaaat acttgacacc cttaccgaca taaatttttt tagtcgttgc ctgcgagcct
     4141 ccggtattgc tccattcacc catagttttt ccaagaaaat aaccacccca gaaaccacgg
     4201 ttaaagactg ttgccagttc atttttccaa tgttcaattt ttgttgcaga atagctgcca
     4261 ttatgataac attcaacagc ttctttataa cattttgttg tagtataaac atagtcggca
     4321 gcacgacctc tgccttcaat tttcaaaata ctgatgcccg tttcaattag tttgtcaata
     4381 aaatcaatgg tgcacaaatc ttttgacgac atgatatact cattgtcaat ttcaagctca
     4441 acttcgtttt ctttatcctg aacaatatat tcacgtctgc aattctgaat acatgcacca
     4501 cggtttgctg aggcattgtg tgagtggaga ctcagataac atttgccaga aacggccata
     4561 cataaagcac catgggcaaa cacttctatt tttattaatt tacctgaagg accttttatt
     4621 ttctgacgtt tgatctgttt tacaacatcg gctacctgat tcaatgttaa ttcacgtgct
     4681 aacacaattg tgtcggcata gtctgcaaaa aagcgaacac tctcaatatt gctgacattg
     4741 gcctgcgttg aaatgtgtat aggcatacca atttttttag catgcatcaa tgccgcatgg
     4801 tcagatgcta taacagcatc tatattattt ttctttgctg catcaataat ttttttcatc
     4861 agcataatgt cgtgatcata cattaccgtg tttagtgtaa ggtatgtttt aacatttttt
     4921 tcgctgcata tttttttaat ttcagcaaga tcactgagtg taaaattatt ggcagcacgt
     4981 gcacgcatat tcagctgctc tattccaaaa tatacggaat cggcaccacc ttgtatagct
     5041 gcttgcaacg actcgaacga gcctgcaggt gacattattt ctgtcattat ttataaagta
     5101 aaataaaggt gtgcaagatg gcagaaacgg atgaaatata aaatgacttg tgtcagcata
     5161 aatagttagg tatgtagcag attgttcctg acgtatagaa atgcttcttt atgaaaaaaa
     5221 gaattcatgc aacattgaat tattttttta atattgcttc acaaaaaatg ataacatgaa
     5281 taaaacattt acactcatta ccacactatt ttgtgctgct gcaattaata caaatgcaca
     5341 gctcattacc atctctgcaa cgcatcagat gcctgctatt ggagatacta taaggtatgt
     5401 tgatgccaac acatttggat tcaaccaaaa cggtacaggc cctgtcacta atacactttg
     5461 ggataatgca ctgcttatga atgcaggcac caactacagt tttttctatg acgacccggc
     5521 aacaataaca ggtaatggcg ttgattcttt tcccggtgca acattagcac gtggtgagag
     5581 tggtgcagca ggttattttt attatcagaa tactgcaaat gatatcaatc gaattggatg
     5641 gtatagcagc gcatctaatt atggcgttta taatacaggt acctatgcca cagaatttca
     5701 ttttccgatg acagcaggcc aaacagttaa ttctacttac tccggacgct atgcaccatt
     5761 cgggttgggc gaagattcct gcacacttga aagcggatcg cttgcaatca atgccgatca
     5821 gcaaggaact ttgattttgc caacaggcac attcactaat gtgttgcgta ttcatgcaat
     5881 tgaaacattt catataaaaa tttatatgtt aggtactccg gtaattgatt atttggtttc
     5941 tgatgattac tactattggt ttcatgatac catcaaacaa cctttactta cctatggtat
     6001 tacaaccatt gacggtacag cgcaaacacc tgttttacgc tatcaacctg tttcaggaac
     6061 tacaggctta aatcagttgg cagcacatac agaaatgacc atcacaccta atccttcttc
     6121 cggtaaattt ttgttaagta atgttggcag cactaatgct acaatagaag ttctgaatgc
     6181 cctcggacag aaagttaatt ttcaacaaac aaacatgggg caatctttgg aggttgattt
     6241 aaataatcaa gcggaaggca tatattttat taaagtaact gacggacgta cttcaactac
     6301 acataaagtt gttgttgggt tttagtttag taagttattg attttttgaa ttggagagtt
     6361 aagatttaag aaatcaacat aaatcttata caaggaaacc gctgttatac taaaaatgat
     6421 ggcggttttt ttcagagaag caactaacac tgatgaaatt tattatttaa actcgagata
     6481 ttttcaatta gtattgagag ttggaataca atttaaaaga caaaaacttt tttaaacaca
     6541 tctatttata aaatatattt tgaaaaattt gaatatacag ctgcaaaaag cgttgttgct
     6601 tttaatgtta ttttttattg ttttcacagc ttattctcaa gatgaaaggg atagcatttc
     6661 aacacataca gttaaagaaa taattataca aggtaatcgt ttagaaacac cttttagtga
     6721 agcagcaaga gatattcaga taattacaca gaaggaaatt gcagctttgc cggttcaaac
     6781 tttaaccgaa ttgcttgttt atgtatcagg tgttgatatg cgtcagcgtg ctccttttgg
     6841 cacacaaggc gacattagta ttgatggagg atcatttgag caaaccttgg ttttgctaaa
     6901 tggtatcaaa ctgattaatg cgcaaacagc acataatgta ttgaacttac caatctcgtt
     6961 ggatgctatt gaccacattg aagtacttcg cggtgctgca gcaagggtat atggtatcaa
     7021 tgcactcacc ggtgctgtta atatagtcac taaaaaaagt aatgaatcat ttgttactgc
     7081 aaatatttac ggaggtacat cttttaaaag aaaagaaacc ggtgatggtg acggcatgta
     7141 tggaggtgga ggggttgact tggttggaaa ctttgcaaat aataatcata gacaactttt
     7201 aggtctaagt cagaatttat ttaatggcta cagatataat tcggcacata aaaacacaaa
     7261 gattttttat gatggtgatt tttatataaa tgaaaaaaat tccataagca cttttgccgg
     7321 acatacctat aacaaatttg gagctaatgg tttttatgct gctcctggtg ataagaattc
     7381 agaagagatt gtagaaacat caatttttag tttatcctct aaacatcagc ttggaaaagt
     7441 caatttaatg cctcgaataa gtgaccgtta tgatgaagat gattatcgct attttaaaag
     7501 tgatttaagc agaggaaggt caaaacattt taccaatgca ctgatgctgg aactaaacgg
     7561 taaccttaca acatctgcag gtgtaatagg tatgggttgg gagtcgcgtt tgacaaagat
     7621 taacagttcc aatattggag aacataaaat ggacaatcat ggagctttta ttgaattcag
     7681 aaccaaactt tttaagagat tagtaactaa tgtcggcata tacgtaaatt ataattcaca
     7741 gtttgattgg aaagcctatc ccggattgga tttagcttat cgtttaaata acaaatggaa
     7801 aattgcagca agttttggct caggtcagcg aattccatct tttacagact tgtatttaaa
     7861 acaacctccg gcaaatgtgg gcaaccctga tgtgcaaccg gaaactgcat ggcagtatga
     7921 tgcgaacatt gaatttactt ccgggcattt gaaagtaaat gtagggtctt tctatcgtaa
     7981 ggttacagac tttattgatt gggtacgacc aaacatgaca acaccgtatt caccaagtaa
     8041 ttttggcaat atcattgtac aaggtattta ttcaagaatt aatcaacagt tgattctaag
     8101 cggaaaacat caattgaatt tttgcatcag ttataatttt ttaaaccctg aacattatga
     8161 ttataaaaac gaacagtcta aatatgtgct ggaatcatta agacatcaat tgattgccgg
     8221 tgttaattat cgttacggta gttttaatgt tcaaattgaa aacagatata ttaagcggga
     8281 gctaaacgat gcttataata ttgttgacgg acgcataagt tatgaaattc attctgtttt
     8341 attttatgca aatgtctcta atatgtttga tgcaaagtat aaagaagcag gtgctgttcc
     8401 aatgcctgga aaatggtttg cacttggcct gaagtacaat tggaaaaaaa agtaaagttt
     8461 atctgaaagc ttttactcgg taggtaaaat gtattatcag agcaatccgg aaattaattg
     8521 aggagctata cctagaatta atgaaagcac tgcgcaaatg cccaatacgg caagatcgga
     8581 tggtctttcg cagtagccag tgttttctgt ttcaggttgt tgaaatgctg caatgataac
     8641 tttaaagtaa aaataaattc cgataagcga acttaatatt gcaatgagaa ccaaccacag
     8701 aagattttct tttagtgctg ccgtaaacaa ataatatttt ccaaaaaatc ctgcaacagg
     8761 aggaatgcct gccatactca gcatacaaat agtgagcata attgctgata caggatgttt
     8821 ataaaacaaa cctttcacag cttttattcc ttcgtttcct gtaactttat gtactgagta
     8881 aagaagatta aatgcaccta atgcagaaag tgaataggct gccagataaa acaacaatgc
     8941 attttttgaa gcaatgcccg gtgctgccaa tgccatgagc atgtagcctg catgtgcaac
     9001 actggaataa gcaagcattc gtttcaggct tgtttgataa accgctgtga tatttccggc
     9061 cagcatggtt atggctgaca gaacagcaaa taccggtgtc cagaaatcat aaatagatat
     9121 gaagcatgaa acaaataacc tgtaaatcat agcaaaggct gcagtcttta ctacagatgc
     9181 cataaacgct gtcactaatg ttggcgcacc atcatacaca tcaggtgtcc agaaatgaaa
     9241 aggtgcagca gcaattttaa atgacagacc aattaatatc atcaacacac caactttaaa
     9301 aaaagcaggt agtgcatctt tattttgttg tgtatagttt gcaatttcct gaatgttgaa
     9361 agatgatgtt gctccataaa tcagtgtaat tccaaaaagt agaaagcctg ttgcaaacga
     9421 acccatgata aaatatttca tggcagcttc gttactcttg atattggttt tgtcacttcc
     9481 ggccatgatg taaagtgcaa acgacaatat ctccaatcca agaaaaagca tggacatgtt
     9541 tgaaaaagtt gtcatcatca caccaccggt gagtgcaaaa acgatgagtg caaaatgctc
     9601 gctcatgcta ctatcactac taaagtaact acgcgacata atcatccaca acaaggttgt
     9661 tacaatcatg agtgatgaaa aaactataga aaaactgtca accattacca tctgattgaa
     9721 gtacgaaccg aaattattcc agtcgtttat tgaaaaacct aatgcaacaa ctaaaccggc
     9781 aatcaccagc gtgtacatta tgcgcttcag tttaaaaact tctgaaagca tggctgcaat
     9841 gccgactata gaaagtgtaa cgatggtatt cacaattaga ttaatttatt tttaaacatt
     9901 atcctgcatt aataatcatt aacagcgatt ttatacttgg tgctgctgca ttcatcagaa
     9961 aagatggata cacacctaat aaaataacca agaatgctat agttcctaaa actgtttttt
    10021 cactcaatgt cattggatga aactctcctt cattcagatg gcggctgcca agcatgatag
    10081 cctgatagct tctgaacata tatactgcac cgagtatgat tgtcaatccg ccaaatattg
    10141 ccatccacgc tttaaactga taaattcctg taagcaacag gaactctcca acaaaaccat
    10201 tagttaacgg aagtgctacc gtgcctaata atactatcat aaacagaaca gcaaacagag
    10261 aattgttatt tgcaatgcca cccatttcac tcatgttatg agtttttaat ctgctcatca
    10321 acatatcagc tataaagaat aatccaacag cattaatacc gtgactgagc atttgaaata
    10381 ctgcaccttc aagtgcttca acttttaaag tgaataatcc ggcagtgatt aggcctacgt
    10441 gtgcaataga tgcataggca atcatccgtt taaaatcttt ttgaactatg gcaatacatg
    10501 aagcataaac cacaccgatg attccaagta acattacgat gaaagcgtta tctgctgatg
    10561 ctccgggaac aataggcagt aaccatctta atacaccata aagacccatt tttagcatga
    10621 taccggaaag taacatcgta ccttgtgttg gtgcatcagt ataggtgtcg ggttgccagc
    10681 tatgtaatgg aaatacagga atctttattg caaaagcaac aaaaatcaac cagaaaacta
    10741 acgtctgttg ttgtgatgaa agcgttaaat cataaaatgc ctgaatatcg aatgtgtgac
    10801 ttccaggtgt ttgcagatag agataaatta aacccacaag cattaataaa cttccggtga
    10861 tggtgtagat gaaaaatttt aaagtgattc gtattctgtc cttgccaccc cacagcaggc
    10921 aaatgaaata aataggtaat agtgcaagtt cccagaagat gtaaaataga aatgcatcga
    10981 gcgaaataaa tacaccaacc aaagcaaact gcatcagttg aatgagtgca taaagtcctt
    11041 taggattttc tattctgcgt ccaaaagatg aaagaataat caatggtgtt agtacgttgg
    11101 tcaataaaat catcatcaac gaaattccat caacacctat tttgaaattt attcctaaat
    11161 ccggcaccca tgcataattt attacatact gcattccacc attaggatca aactgaataa
    11221 acaagcaaac tgaaagcaat aaactgatga gagaaaatac aaatgctgtt tttcctgtcg
    11281 cttcacgtga aggcaacagc gacaatgcca tgccggcaag tggtaaaaat aaaattatca
    11341 gtaataaatt attcatctac agcagcattt taaatacaaa aatcaaaatg attcctatga
    11401 ccatactcac caaatagaat ccaacacttc cgttttgtaa taatcttaac ccggaagaag
    11461 tcaggtttgt ggctcgaggt ataaaattta caattccatc aatgatacta atgtcaaata
    11521 ttttataaaa caattcgcca aacgcttcta acggttttct gattatcata tcgtataatt
    11581 catcaacata atatttgttg gcaagtactt tttggaatga ttttgtttga ctgtcatctt
    11641 caggcaccac tttatctctg acatacatat ttttagtcat aacataaatt atggccaatc
    11701 caattatagt gcctatcaac aaagtaaact caaatgtatg cgataaaata aattcttttg
    11761 gagaataaat aattggatta aggaactgcg taaacacgtg gtgcgttcct aatttttcat
    11821 tgatatattc cggcatgccg atataacctc ctattacaga gaaaacagca agaataatta
    11881 atggtacagt catgctcgaa ggtgattcgt gaagatgctc tttctgatga tgtgtgccac
    11941 gaaaatcacc ataaaaagtc aaccagtata aacggaacat ataggttgca gttagtaccg
    12001 atgtgattgc taatatggta aacatcaccg gtgaataagt ccaggttact gcaagaattt
    12061 cgtctttaga aaagaatccg ctaaacaacg gaacaccact aattgccaaa actcccatca
    12121 ggaaagtaaa atgcgttatc ggtaaatatt ttttcaaacc ccccatgcgt ctgatgtcct
    12181 gttcgccact catggcatga ataacactac cggcacataa gaaaagcaat gctttaaaaa
    12241 atgcatgtgt tactacgtga aacagagcag tggtataagc accaacacct aatgctacaa
    12301 acatatagcc caactgactt acggtagagt aggcaagtac ttttttaatg tcattttgtt
    12361 taagacctat tgatgctgcc atcaatgctg ttactaatcc tattacagcc acaatctctc
    12421 ctgcaaatgg tgcaagcgta taaattacat tgctgcgaat aaccatataa acaccggcag
    12481 ttaccatggt agcagcatgt atcaaagctg atacaggtgt aggtcctgcc atggcatcgg
    12541 gcaaccaggt atagagtggt atttgtgcac tttttcctac tgcaccaata aaaagaaaca
    12601 atgcaattgc agtcagcgta gcatcacctg aaggatagtt tcctgcagca gcaaaaacat
    12661 ctttaaattc taatgatgaa aatgtctgat acattaaaaa caagccaagt aaaaatccta
    12721 aatcaccaat acgattcatg atgaatgctt tgtttgctgc tttattatag tcgttgtttt
    12781 taaaccagaa acctatcagt aaataggagc aaagaccaac accttcccat ccggcaaaca
    12841 ttactacata gttacttccc attacaagca gcagcatgaa aaagataaac agattcagat
    12901 atgacatgaa ccttgagtaa tcttcatcat gtttcatata gccaatagaa taaacatgaa
    12961 tcagaaagcc tacaccggta acaactaaaa gcattataac cgataagcga tcaactaaaa
    13021 atgaaagacc cagattgagg ctacctgcat tgatccacga aaacatggaa taatacgatt
    13081 ccttaatcga gccattgttg atttgcaaaa agagatagca ggaaattaaa aaagaaaata
    13141 gaacagtgcc gcttgcaata atgcctacaa gactttgcgg cattttttta ttgattatgc
    13201 cgttaagcag aaaaccgatg agcggaacaa gaggaattgc tgatatcaaa aaatccatta
    13261 cctaccattt aagtttattt aaaacatcta tatctgttga cttaacatta cgataaatca
    13321 taaccagaat agctaatcct actgcaacct cggcagcggc tacggccata ataaaaatca
    13381 caaacacctg acccgatgca tcattatgct gattgctgaa agcggtcaat aggagattca
    13441 cagagttaag cattagttca atacacataa aaataattat ggcattgcgt ctgaataata
    13501 ctccggcaac gccaatacaa aaaagtatgg cagagagcag caggtagtga tttatattaa
    13561 ccggttccat tttatttaac ttcttttttt ccaagcatca cagcaccaat taatgcagtg
    13621 agcagtaata ttgaaaccat ttcaaatggc agcatatatt cattgtatag tacatgtcca
    13681 atggattcta ccaaacctat atctgtttgt gagttcaatt tggaagattc tacatccaca
    13741 tgcttgagta cacttacaat tacaatcagc aacagaccac tggatatgac ggctgcaaat
    13801 tttgaaacaa tatttttatt gggttcaatt tctttattaa ggtttagcat cataatcaca
    13861 aacaaaaaca aaaccatgat agcacctgca taaactatta aatgtactgc agccagaaat
    13921 tgtgcattaa gcagaatata atgaccgaat aatgtaaaga agaagaatat cagatacaat
    13981 acactgtaaa ccggcttttt agaaaatacg gtaaacaagg cactcattac cgaaagtgct
    14041 gaaagtatgt aaaatgcgta ttggctcacg ttagtattat agaatgaaaa actggtgcga
    14101 aattattgtt ttaattgatt atttgctatt gattaatttg ttttttccgg gcaatgatat
    14161 atgtcagtca caactacatg tacattgtaa ttgggataaa acatgcttgg tgaatcatat
    14221 tcgtattttt cttccttgaa tttatcgtct ttaaaatctt tttctttatc gcattgctgc
    14281 tcagttttta cggtgtcacc gggtataata tatgctgcaa gcgtagtgtc ggtataggta
    14341 cacaacatcg aacgcaccaa atagcaatcc ctgcacacat ctttatcgca agacattaag
    14401 ccgagtgata aagtgataat aactccggaa aaaagttttt tcaaagaacc catcattcta
    14461 cttcttttct atgtgctctt gctctgtagc ttatatctac tcgttcttcc ggtgcaatac
    14521 cttcaaccaa tttgtcttta ccgtaaataa aattatcgcg caaagtatcc gatggtgtca
    14581 tgcggttggt taaaaatatt gcttctttcg ggcaggcttc ttcgcataat ccacagaaaa
    14641 tacagcgcag catattaatt tcataaacag cagcatattt ttcttctctg tacaaatgtt
    14701 cttctccttt tttgcgttca gcagctgtca tagtgatggc ttcagcaggg caggctactg
    14761 cacacaatcc gcaggcagta cagttttctc taccttgctc atcgcgtttc agaacatgca
    14821 ggccgcgcca aacttcactt attggctttt gttgctcagg aaaattaata gttgcttttt
    14881 tcttgaaaaa atgtttaata gtaatcatca gtcctgctac aattgcagga atgtatatct
    14941 tctctgcaaa tgtcatttct ttgttgacta ctactttact tctattggtt aacatgatta
    15001 attataataa taaccgttta caaatgtaat gaatgttgtt tacttttttc tttttttgaa
    15061 tggtgatttt gtagttatag aaccatctgg cagtctgtaa aaaaccatat ccatgattcc
    15121 gaaaacagtt ggtatttttc tttttttaga atcaatcatt gctaattgca caccccggtt
    15181 accgatggct gcaaattcca atgcttcttt aaacaaattg ctgtttttat aattcttaaa
    15241 cttactactt ttcatttttc aatatctttt ataaataaat caaataatga ttgattgaca
    15301 atgtcatatt tattcttctc tccggctgct attatctcaa atcctgattc tgtgttgtta
    15361 aataatttcc agtcttcaac caaatcttta taaattttcc agaagttgtt tttccctctt
    15421 gtaaaccttc tcctaacatc atcatctggt acaaaatgtc ctcctgtttc aactctatgt
    15481 tttatccttc ctatacattg ttccggagca tttaatacta taaaaacaat ttttatactg
    15541 taatctttct ttttaaactc tctgatcaat tttctcagaa acaaacctga caaagttgat
    15601 tcaagaatga tgtttttatt ttttgctttt aatttttcta tccgtttaaa gtattctttt
    15661 cctgatgaaa tatttcctgc cctgctacca tcatttgata attctcttgc aatttcatct
    15721 gcatttaaaa attcgtattt atattcctgt aagaacgatt ttgcaaaagt tgttttacca
    15781 cttccatttg gtcctcctac tattaataat tcttttgtca cttttcatct gatgcttaaa
    15841 aatgaaacag tccgtttttc cataacaaga acacaccggt cagcaaaacg tttagaatag
    15901 ataatggaat gagcattttc caaccaaggt tcatcaactg atcgtatcgg aagcggggta
    15961 ttgtccacct tacccacata aagaaaaaga taaagaagaa tatttttata aaatagaata
    16021 atacacctag caatgtcaat gcattatgcg ataatccaag ttcactgata aatggcatgt
    16081 tgaatccgcc aaaataaagt gcagaaataa cagcagatga aataaacata ttgatgtact
    16141 cggcaaataa atagaagcca agtttcatcg aagagtattc tgtgtgatag cctccaacca
    16201 actctgtttc gcattccggt aaatcaaacg gtgtacggtt acattctgca aaagcacaaa
    16261 tcagaaaaat taaaaatcct aatggctgat aaaatacatt ccagtgaaag ccctgctgct
    16321 gaacaacaat ttcacgaaca ctcaaagtgc ctgtaaccat tatcaatgca atgattgaca
    16381 ttcccattgc aagttcataa ctgatcattt gtgacgatgc acgaaccgaa ccaagcagag
    16441 agtatttgtt gtttgatgcc catccgccaa tcattatacc gtaaacacca atagaaacca
    16501 caccgaaaat atatagtaca ccaatgttta catcggtaat atgcataaag tattcttttc
    16561 cattgataat taaagtatcg ccccatggaa ttacgacacc tgtcatacaa gctgtaagca
    16621 tcatcaggct tggacctaat ataaacaatg ctttatcggc atgtgatgga ataatttctt
    16681 ctttcataaa catttttaca ccatcagcta atggctgaag aataccgaat ggccctgcac
    16741 ggttaggacc aactctgtct tgtaaaaatg ccgccacctt tctttctgca taggtcgaat
    16801 acatggctat cagcaatgaa accagaaaga cagcaattac aaaaactgtt ttgaatatta
    16861 ataccgaaac ttccatttct aaaatttaat tatgtgcttt ttctcctaat tttttctgac
    16921 gtgataaatc ctgcttcagt tttttcaact cttcgtaatg attttgtgag attactgaat
    16981 ggcggtcaat gtgacgtggc ccttcaataa cccagtcgct tgtttttttc ttatcgaagc
    17041 gacattcatt acaaataaat tcatccactt ctccccattg atcttttctg cctgtaacac
    17101 gaagcacttc ttctccctga taccataata caactttgcc tgaacatgtt gggcagtcgc
    17161 gatgtgcatc tacaggtttg gtaaaccaca cacgactttt gaaacggaat gttttatcgg
    17221 ttaatgcacc aacaggacaa acatcaatca tgtttccgct ccactcattg tcaacagctt
    17281 gttcaatgta ggtagatatt tctgaatccc atcctctgtt taaaacacca tgaactcttt
    17341 caggacataa ttgctcagca actttcacac agcgataaca catgatacat cggttcatgt
    17401 gcagtttaat tttatcgcca atatctatca tcggaaaagt tcttttcctg aaatcaagac
    17461 gtgacacttc atggccatgc tcgtagctta aatcctgaag attacattct cctgcctgat
    17521 cacaaaccgg gcaatcaagt gggtgattga ttaataaaaa ctcaactact cctgcgcgtg
    17581 catctaaaat ttcaggcgat gtaatgtttt gtacttccat accatccatt acagtctgtc
    17641 tgcacgatgc aacaggtttt ggcattggtc gcggatcttt ttcagagcct ttacttactt
    17701 taaccaaaca agtgcggcaa taaccacctg acgttttcaa tttggaataa tagcacattg
    17761 ttggtggcac aatatcacca cctatttttc gggctgcttc cagtatggtt gttccttcag
    17821 gaacttcgat ggtgtgacca tctatagtaa cttttggcat tatgcttctt gtaaattatt
    17881 tagtctgtaa taatgtttca cgtctttcat tccgtgattg ttgatataag cttcaaactc
    17941 gcttctgaag tgtctgattg atgatgcaac aggccatgct gctgcatcgc ctaaaggaca
    18001 aattgttttt ccttcaatat ttccctgaat gttccacaac aaatcaatat ctttcatttc
    18061 acctttgccg tttagaattt tatttaatat tctgtacatc catcctgttc cttcacggca
    18121 tggcgaacat tgtccgcaac tttcgtgatg ataaaaatgc gcaaacgtcc acaggttttt
    18181 tactacgctg gtatcttcgt ccatcacaat aaaaccacct gaaccaagca tggtgcctgt
    18241 agcaaatcca ccatcggcaa gactttcata agtcatcaga cgaggttcgc ctgctgcagt
    18301 ttttaaaatg aggtgtgcag gtagaacagg aacagaagag cctccggcaa caacagcctt
    18361 taactgtttt ccgtttttta ttcctccgca gtatgcatcg ctataaataa attcttcaac
    18421 aggaacaccc agttcaattt cataaactcc gggtttatta atgtgtcctg atgcggaaat
    18481 taattttgtg cctgtacttt ttccgatacc gatttttgca tattcttcac cggttatttt
    18541 tattatcggt acaactgcgg caatggtctc tacgttattt accactgttg gacatcccca
    18601 caaacctttc actgccggaa aaggaggttt aattcttggg tttcctcttt taccttctaa
    18661 tgattctatc aatgctgttt cttcaccaca gatatatgca cctgcaccac agtgtacatg
    18721 caggtcgaga ttaaagccgc ttcctaaaat attttttccg agatatcctg ctgcataagc
    18781 ttcgtcaatt gctttttgaa gtatgtgaaa tacatacatc atttcacctc tgatgtagat
    18841 gtaggaagta tttgccccta aggcaaaact tgaaattatc attccctcaa ttaaaatatg
    18901 aggtttgtgt tccatcagat accggtcttt aaaagttccg ggttcacttt catcggcatt
    18961 acacaccaaa taacgtggca caccttcggg cttagcaatg aaactccact tcataccggt
    19021 tggaaaaccc gcaccgccac gtccacgcaa tcctgatttt tgtacttcac ttacaatttc
    19081 ttcaggcttc atttgaagtg ctttgcgcaa agcttcgtat ccgccattgt cgctgtagcc
    19141 ggttattgac tgaatgcccg gcttatctaa gttatctaaa agtaatttac gtgacatact
    19201 ttttctttca gggttttact ttatcttttt cccagtgcgt tcttctgttg ccctggtttt
    19261 tatgatcatt taaaatatca tcaacttttt ctaaggtcag attttcgtga taggtttctc
    19321 cgatttgcat cattggtgct gtgccacaac ttgccaaaca ttcaactgtc tttaaggtaa
    19381 acataccatc ggcagttgtt tctccggctt tgattcctaa tttcttttca atataacgaa
    19441 caacatcttc tgcacccatc agccaacaag gtcctgtgcg gcaaacttct atcagacact
    19501 tacccacagg tttcatgttg aacatggagt agaatgtagc tacttcataa acttcaatag
    19561 actgtatttt tagtaatgat gctacataat ccatcacttc aggacttaac caaccgtcaa
    19621 attctgcttg tgcaatatgt agtattggaa gcagtgcaga tttttgtttt ccttcaggat
    19681 atctgtttat tattttttga accaatgcca tagcatcgtc cgaaaattgt atgtttttgt
    19741 ttgtgctcat gttacatcat taatttatgc atccagttca ccggctatta cattcattga
    19801 actcattgtt aatatagcat cactcaaata ggctcctttt atcatttcag gataggcttg
    19861 ataataaata aagcaaggtc gtctgatatg taaacgataa ggagtacgac cgccatcact
    19921 gattaagaaa tatcctagtt caccgtttgc accttcaaca caatgatata cttcgccttt
    19981 gggtacagca gtttcggcca taataatttt aaagtgccat attaaagctt ccatttcatt
    20041 atacacttca tttttaggag gtaaataaaa ttcaggcact tcggcattaa aggaagtttt
    20101 atcttccagt ttatctaatt tttgcatggc ttgttttatg atgctcatgc tttctttcat
    20161 ttcttgttcg cgtaccataa aacgatcgta ggtgtcgcca ttggttccca aaggaatcat
    20221 aaaatcaaac tcttcgtaag atgaatatgg attcatgaca cgaacatcat agtcaatgcc
    20281 ggcagctcgg aggtttggtc ctgtgaaaga ataagccaaa gccatttcag cagaaattgg
    20341 tccgcagttt attgtgcgat ccataaaaat acggttgcgt atcaataggt cttcaaattc
    20401 ttttaatcct ttaggaagtt cttttaacag tttttcaatc ttcaaccaag cagtctgcga
    20461 aaaatcacgt tctaatccac caattcttcc aatgttagtt gttattctgg ctccgcatat
    20521 ttcttcgtaa atttcataga tatgttcgcg aaactgaaaa acgtataaga accctgtcat
    20581 agcgccactg tcaacaccaa taactgagtt gcagataata tgatcggcaa tacgtgcaag
    20641 ttccattatg ataacacgca gatattctac tcgtttggga agtctaattc ccgccagttt
    20701 ttctactgtc atgtgccatc ccatattgtt aataggtgag gaacaataat tcaaacgatc
    20761 ggtaatagtt gttacctgtc catagggtct gcgctcggca agtttttcaa atgcacgatg
    20821 aatatatccg atggtaggtg ttgattcaat aattatttca ccatccattt taagcacatt
    20881 ctgaaaaata ccatgtgttg ccggatgtgt tggcccaagg tttagggttg tatattctct
    20941 accgataagt tcttgtttga tttcttcttt cattttttac aagtaaatta acgtccaaac
    21001 attctatc
//
LOCUS       DAOOOI010000042        19286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_566_length_19286_cov_17.659924, whole genome shotgun sequence.
ACCESSION   DAOOOI010000042 DAOOOI010000000
VERSION     DAOOOI010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 19286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19286
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_566_length_19286_cov_17.659924"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PKX59_06685"
     CDS             <1..812
                     /locus_tag="PKX59_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOZ90590.1"
                     /translation="RGGRRVNRGSNLRIKVKLTLQEIANGVEKKLKVSKKVACHTCHG
                     SGAQGNNAFTTCSACKGSGQVRRVTNTILGQMQTVTTCNVCGGEGQVIANTCRTCHGT
                     GVEQGEDMITVPIPAGVADGMQLNVSGKGNLGERNGIAGDLLIVIEEIEDPKLKRDGD
                     NLLFDLYITFIDAALGTQVEIPTVDGKAKIKIDAGTQGGKVLRLKGKGLPRVNSYHKG
                     DLLVNINVWTPQQLSAEERKMLEKLRDSENFKPNPGKGDKSFFEKMKEFFN"
     gene            complement(1262..2065)
                     /locus_tag="PKX59_06690"
     CDS             complement(1262..2065)
                     /locus_tag="PKX59_06690"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ90591.1"
                     /translation="MKHQGGSLAGSGLGGGAGIWLATHYNLTLAGIVLCMVSVLFALT
                     MFLIKDIPFKKQKSIILEIKGMGINIFSMIKLPVALFVMILILMPIGTGAMSNLWSAV
                     AQDWKADADTVALVTGILSGIVSDVGCIAGGFISDRWGIWNAYLGIGVICALVTVLMA
                     FMPYTPPAYISGVLVYGFCMGLMYAAYTAVLLFVIGKSHSATKFSLMSSLGNMPVVYM
                     TTFNGWAHDKYGSKFMLSAEAFIGIIFIVIFYLILLRMKKKNMIPLIVD"
     gene            complement(2046..2411)
                     /locus_tag="PKX59_06695"
     CDS             complement(2046..2411)
                     /locus_tag="PKX59_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90592.1"
                     /translation="MGISQGFITVTLPFMLTQNGFSVALTAGIVAIGISANLWRFVWG
                     PIVDLSLSLHKWYWLSMSASIASLLLICSIPFTIKGETLLSVIVFISQVAATIALLPL
                     NGIMAKQIHENKKGEASGW"
     gene            complement(2742..6917)
                     /locus_tag="PKX59_06700"
     CDS             complement(2742..6917)
                     /locus_tag="PKX59_06700"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90593.1"
                     /translation="MFKNLFFSKGVKSIALAAFCLLTFTLGANAQILAWEFNGAAGNE
                     TTIAATTLDANLNSSSVSRGSGVNASGLTNAFASTDYTVSGTFANAVTNNEYQQFSVN
                     AKSGYKVSLSTLDVNFRRSNTGPNTFQWQYSLDGFATAGINIGSAISYTTSPTNGTAQ
                     SQIDLSVISALQNVSNATTITIRLYGYGASAGTGTFALGRLTGNDLAIGGTVDPDGGS
                     PVLTVGTLSNQFGNVCIGTTTNYETFTVSGTDLDGSSVDVGTLSGFTYCLTPGGTYTS
                     TLNLPYTAPTLSSTTVYVKFSPTLVQSYAGSIPVTGGGASAGSCSVSSASGINTSPSL
                     TTGSATGITQTTATCEGTITDNGCTSVSAYGIEYSTTNNFTPGTGTQVSSSNISGNTY
                     TSALTGLAGNTTYYYRAYATNTAGTGYGAQASFTTDAFTAPVATAATNIGIHSFRANW
                     NSVSGATGYFLDVSTSSTFLAPVTTDLVYWDFPNDPDDATADGGILSNLSATISSTAG
                     GTMTFSTNGASTKSASINNWDNGNGTKYWEVSFDATGYYNLAFSSKQQSSNTGPRDFK
                     VQYKIGSGGTWTDVTGSSVTVANNFTSGVLTDIALPAACDNQSSVYLRWIMTSNTSVG
                     GGSVAATGTNRIDDLFVTGYEGDFVLENQSVAGLYYDVTGLNPITTYYYRVRATDGVA
                     TSDNSNVINLTTLDVCDLFTVTASSNAPICSSTNLNLNANASGSDGTITGYEWSGPGG
                     YSNMTQNPSVSNPLAGTYTVTVTDDNGCTVTDNVSATVEAAPTANAGSDDIICSSGSA
                     ALNGIIGGDATGGTWTTAGDGTFSPNATTLNAAYVPGTTDISNGSVVLTLTSTGSVLC
                     SAVNDQVTITIQTGVPSQPAVVSGAPTSVCPPAGPYTLTTSSTNANSYNWYVVGANTG
                     ITFLSANGSNSMDVQYGVTANSSYSVRVEASNACGTSSYRSAFTRRSVSTPATVSGNK
                     VACATTSDTYTCTAVAGAESYQWSITGDATVSGTTNSVTVNFGPAWTGGTLCVSSQVG
                     CFTSPSKCMTITSSTPAVGAISGSATACPNGVVSFSVAPLAGIQTYNWTTPPNSSVTL
                     GSGTNNVQVTFNGSYNGVGNICVSATSICGVTTAPKCKTVAPGLPSVPASITGGTNGL
                     CNQSGNFSCPLQAGNTYNWTAPGTINNNGSNSVNIQFSNITTGSVCVSASNSCGSSAY
                     RCVTVKGAPNSPVALTSNPAVICANTTGVEFNANTSNTTGAYTLTWSYTPAAATYNSG
                     GGNTNQLILDWGNTNGVVVVTASNACGSGAKSYTVNLGCRQQGQDDVAALNIYPNPAS
                     GTVNINLNAGNQQAVQCIITDLSGKVVMQQTYNVAVGNNTFSADVSHLAKGIYMLQIA
                     GNTMQATQKLIIQ"
     gene            complement(7038..9122)
                     /locus_tag="PKX59_06705"
     CDS             complement(7038..9122)
                     /locus_tag="PKX59_06705"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ90594.1"
                     /translation="MRLLSFVLCFMFCLNVSVWAQQAESDSLQHLLRTQADDTSKVNT
                     LNELSYVYYQTAVYDSELYYAQQALNLAQKLNWKRGMAVSLKNIGSACDDMGNNSDAL
                     VYYGRALNIFSSIDDKSGVSACYNNMGLVYRTLGNFSEALKNHFASLKIKEAIGDKNG
                     IALSYNNIGVVFNAQKNYDEALKNHRAALAIRLSLGNKRGIASSYGNIGNVYQAQGNN
                     TEALKNYTEALKIAEETGDKVGMTTALNNMGAAYFEMGDYENALQHNTDALRIAEEAG
                     DLSSIAAASINTGNVMVQMKQPQAATEWLQKALEIGKEIGEVEVIKNSYEGLSQADSA
                     LGKYSDALQHYKMFIRYRDSIVNEENTLKTVQLQMQYEYDKKDAIARAVQEKKDEVAQ
                     QEVQKQKLLRNSFIAGFALMLALAAVSYRSFVRKRKDNHLIEKQKLLVEEKNKEITQS
                     LHYARHIQAAVLPNQKNIAALLKNHFILYLPKDIVSGDFYSVLQNKDKVIVAAADCTG
                     HGVAGAFMSMIGTSLINHLVNEKNITNPAVILDELNEGIINSLKQRQGDSNDGMDIGL
                     CSIDFKENKLLFAGANRPLWLFRNEEIKVIKPDKFPIGGMQVDHKNKYTAHSIDLLPN
                     DTIYIFSDGYSDQFGGEQGKKMMSKRFKQYLQVMQHLPMDKQKEQLEQLFNSWKGNHE
                     QVDDVLVVGIRV"
     gene            9256..10185
                     /locus_tag="PKX59_06710"
     CDS             9256..10185
                     /locus_tag="PKX59_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013410135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ90595.1"
                     /translation="MNLLSIREVSKIYANHKALDSVSIEIPESSVFGLLGPNGAGKTS
                     LIRIINQITGPDGGSILFGGQTLNPSHISEIGYLPEERGLYKKMEVGEQAIYLARLKG
                     LSRADALKKLRYWFEKFELQSWWRKKVEELSKGMQQKVQFIVTILHEPKLLILDEPFT
                     GFDPINANLIKDELLGLRQKGATIILSTHRMESVEELCTHIALINKSKKILDGQVKDV
                     RKQFRSNLFEIEYKGTSVGFANALWTGFELVETRAQDDHAFAVIKNLNNSSTNDLLRQ
                     LLNEVEVVSFRERIPTMNDIFIESVQNNKPVEA"
     gene            10182..11498
                     /locus_tag="PKX59_06715"
     CDS             10182..11498
                     /locus_tag="PKX59_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ90596.1"
                     /translation="MNIILLIIQREYLTRVKKKSFIIMTILGPLLMGGLIAGFAYLSM
                     NQGDSISMIRVVDETGVLAGKLNNSKTVIFTKDTMPIQTARHLFNSNTDYGILYLPAN
                     ALQKPDATVLYTEKQPNISVVEYLESELSKKIENMKLTDAGIQKSTLDSIKTDISLQQ
                     KPVDPSGKDKSFSAGVSAGIGFGAGLLIYMFIFMYGIQVMRGVIEEKTSRIVEVIISS
                     VKPFQLMMGKIIGIALVGLTQFLLWVVLTMAISGTATSLIVGKDQSKLMEMQQKQMVP
                     NANIPQEKMQPASMDFMKIFEGFNITKILLCFVFYFLGGYLLYSALFAAIGAAVDAET
                     DTQQFMLPITIPLIFAYVVSTIVMTNPESELGYWFSIIPFTSPIVMMVRMPFDPPIID
                     IVISMVLLVAGFIFTTWIAAKIYRTGILMYGKKPTYKELFRWLFYK"
     gene            11504..12424
                     /locus_tag="PKX59_06720"
     CDS             11504..12424
                     /locus_tag="PKX59_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HOZ90597.1"
                     /translation="MNIAVIDCGTNTFHLIIAGISEGSFKILYRKNLAVKLGEGGISN
                     NKIARAAFGRGIDALKEFAADMKQFIIDKQLCYATEAIRRAANGKDFVEEAKKQSGIT
                     LEIIDGLREAELIYSGVSHAVPFGKEKVLIMDIGGGSTEFVIAGNGKMLWRKSFPLGA
                     ALLLDMFKPSDPIHEVQEKIITEHINSVLFPLTEALKQYGPVNTLVGSAGSFDTFLDL
                     CLHCFHKIKANTTWWPLDSEEYFDVHQILLTSTLQQRLEMPGMIAMRADMIVVASMLL
                     HYVIHKHNMNKLLVSAYALKEGMLYFAMNK"
     gene            12455..13615
                     /locus_tag="PKX59_06725"
     CDS             12455..13615
                     /locus_tag="PKX59_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOZ90598.1"
                     /translation="MSRILIIDDEKSIRHSLKEILEYENYKVDEAPDGIEGIKMAEKE
                     KYDIIFCDIKMPRMDGIEVLENLQNKCPEVPVVMISGHGNIDTAVEAIRKGAFDFIAK
                     PLDLNRILITVRNALDRTVLVKETKTLKRKISRTNEMIGESEGIRKIKDMIDRVAPTD
                     ARVMITGSNGTGKELVARWIHEKSSRNNMPLIEVNCAAIPSELIESELFGHEKGAFTS
                     AIKTRSGKFEQANGGTIFLDEIGDMSLSAQAKVLRALQENRITRVGGEKDFPVDVRII
                     AATNKDLKSEIEKNNFREDLYHRLSVILIHVPALDDRREDIPVLTNYFLKEIADEYKS
                     PAKEIAPDAIEQLQKKNWTGNIRELRNVVERLVILGGKTITKDDVINYVGVK"
     gene            complement(13687..15030)
                     /locus_tag="PKX59_06730"
     CDS             complement(13687..15030)
                     /locus_tag="PKX59_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOZ90599.1"
                     /translation="MKDMKRCNQFSLTLFLMLFALFANAQKHKTLDQFTDIDLRGSLN
                     AVYKLDSTWSIEVPEDNVTTQWSLENKTLVLSGAGSNIIVRSPDLQKINISGSGSISA
                     EDTMELHDLNIDISGSGKFKMNYVKAKHIEANMRGSGKIILGGSAQSLNANLAGSGKI
                     ITHKLNVDRAEVNVSGSGTSSVWVSDSLSANIAGSGNLYYVSSPRTINQAIAGSGKIE
                     PITSAIESELNSNSITISGSNKSGTYWSGFELGFNGLYNSKSKFDTPDGYDFLEMIPE
                     KSLAVNINFYEYDVKLIKRYVIASTGIGLSYNNYRFRKNMSLIPDTNALLYTQDTMNL
                     RKNKLTVSYATVPVLLTFNTSSNLKKAFHITTGLLFSYKLGSHTKKVYNKDGKKEKDK
                     TFDDFNIDPFRYDATVRIGYSDFTVFANYALSELFKSGKGPELHPWTIGVSLVPW"
     gene            complement(15121..17046)
                     /locus_tag="PKX59_06735"
     CDS             complement(15121..17046)
                     /locus_tag="PKX59_06735"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HOZ90600.1"
                     /translation="MLVKKLFLILFSVATTLTCAASVGDTTFVSSHTNDTVVTNPNTG
                     SNGYPNWVVFPSASTNYRKIILTLTHRCPSPMSCGEWDYLDYIYIRRTGGQNNPSQDI
                     ELARYITPYGNTFNSTFKSSWTLDVTDFASFLHDSVEIEYIHTGYETNVGRGWLVSVD
                     FALIEGTPVMNPIAFKRLWHGSFPYGNASNPIENYLGADTVTLNFNTANLRLNVTQSG
                     HGADANYCAEFCSKTRTIYFDNSLVDTKQVWRLCGTNPIYPQGGTWVYDRANWCPGNW
                     VYPAVNNFAVAGGSTHIIDMNMQNYNVTSPSANYVIEGQLFEFGPLNNNIDAAIDEIL
                     APTDKFEFSRSNPTCNNPKIRIKNNGSTAITSATIKYGLVGEPESTFNWTGSINTFAT
                     ADVDLPGLISYTSGNRIFKAYIADVNNTADQYVYDDTAYSNAYVPEVMDTVFILLVKT
                     NNYPTENGYKLYDENNNIIVQRLPGSMAANTIYRDTIHATPGCYRYEITDSGDDGLYW
                     WANSAQGTGYHRILRLNNSIARNFERDFGSRYSYRFVSAPGAVVGINNQERYADIVVY
                     PNPTKGIINADITLTSASPLEVSVYNPLGQRVAVQNRTNFNAGIITFDLENQPKGVYL
                     VKVVANDQVKVQRVVLE"
     gene            17249..18472
                     /locus_tag="PKX59_06740"
     CDS             17249..18472
                     /locus_tag="PKX59_06740"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOZ90601.1"
                     /translation="MKKFISGFSILFLFCSLHVSGGGFQMQPQSHSSAGMAGAVIALQ
                     TDGSVIFYNPGGMVFLEKSNVFGGISYSQSRMAFRSNNSGLVNRTESKNMLPFYVGGT
                     IMLSEKMAAGIVVNTPFGYNTTWKSDWEGRNIILSAKLNTIFIQPTFSYKISEKLGIG
                     AGFVFASANYKWNRALIVADGADAQLDGKATGFGFNAGINYAINKKLTAALAYRSKVK
                     LNFKNTDVTFSNIPASLTNQYPASANAAAEMPLPSVLSIGFSYEVIKGLKLSFESNLN
                     GWSSFDSLNYEFDAPNTALNQEFRQARKYRDALTLRLGANYKISDKVTGRAGIAFDQT
                     PVKENYMTPDMPDGDRYVFGIGASFNVSKTFVIEGSYNFETKAEREGVNKDTGLNGAF
                     NALSHCFGVGVNYSF"
     gene            18500..>19286
                     /locus_tag="PKX59_06745"
     CDS             18500..>19286
                     /locus_tag="PKX59_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90602.1"
                     /translation="MSNNHILKSLLLAVIMIAGCKPELKELKFDNNSIDFSKYVAIGS
                     SYTAGYQNNALFDMGQQNSFAAILASQLKKVGGGEFLQPLVSGNDGAGFNFFLQTYIP
                     QTKLGYGTTCSSAPGFSTFPSNVTDIVAVETYIGNNAPYNNMGVPGMKSSDLDNANFG
                     STDLLLSKNPFYARFATNPETSTVLTDAVRQLPTFFSFWVGFNDIYDCAMAGSNQTLA
                     TITPLANFQNNINNALTSLIAGNRKGVVANIPDLLDLPYFTSIA"
BASE COUNT         5864 a         3642 c         3945 g         5835 t
ORIGIN      
        1 agcgtggagg ccgcagagtt aaccgtggct caaacctgcg tattaaagta aaacttacac
       61 ttcaggaaat tgccaacggt gttgagaaga aattaaaagt aagtaagaaa gttgcctgcc
      121 atacctgtca tggtagtggt gcacagggca acaatgcatt tacaacatgc agtgcctgca
      181 aaggttcggg acaggtgaga cgtgttacga atactatttt aggtcagatg caaacagtta
      241 ctacctgtaa tgtttgtggt ggcgaaggac aggttattgc caatacctgc cgcacttgtc
      301 atggtacggg tgttgagcag ggagaagata tgattacagt acccattcct gcaggtgttg
      361 cagatggtat gcaacttaac gttagcggaa aaggtaatct tggcgagcgc aatggcattg
      421 ccggtgattt gcttatagtg atagaagaga ttgaagatcc aaagctgaaa cgtgatggtg
      481 ataatttgct gttcgattta tacatcacat ttattgatgc tgcattaggc actcaggttg
      541 aaattccaac tgttgacggt aaagcgaaaa ttaaaattga tgccggcaca caaggcggca
      601 aagtgttgcg attgaaagga aaaggactac caagagtaaa cagttatcat aaaggcgacc
      661 tgcttgtgaa tattaacgtg tggacaccac agcagctttc tgcagaagaa agaaagatgc
      721 ttgagaagct acgcgactca gaaaatttta aacctaatcc gggtaagggt gataaaagct
      781 ttttcgaaaa aatgaaagag ttttttaatt aggcattgag gttggaaggt tggatttgat
      841 aatttgataa tttgaaaatg tgttgatttg ataatttgat aatttgaaaa tgagataatc
      901 agccacggct gacaggtttg aaaatgggag tttggaaagt ttggaaagtt tggaaggttg
      961 ggaagtttgg aaagttggaa agtttggaaa gttggtgttt acatagttat ttaatgtttt
     1021 caagaagcct gcgcacaccc atagccataa gaggctttgc tttttacaaa atgcctaaag
     1081 ggcgctgaca cacgataagc aattaaactg taaactttat ttaggaaaag acactgacat
     1141 ttgcggtgct gaggttggca tttgcggtgc tgaggttgga gtttgcggtg ctgaaggttt
     1201 ttaatttagt ttgattttca gctatatata cacaaggtta cacaacttgc catttacccg
     1261 attaatcaac aataagaggg atcatgtttt tctttttcat acgaagtaat ataaggtaaa
     1321 aaatgacgat aaatattatt cctataaatg cttcggcaga tagcataaat ttacttccat
     1381 atttatcatg agcccaacca ttgaaagtgg tcatatacac cacaggcata ttgcctaaag
     1441 atgacattaa agaaaattta gttgctgaat ggcttttgcc gattacaaaa agaagaacgg
     1501 cagtgtatgc agcatacatt aatcccatac aaaatccata aacaagcact cctgaaatat
     1561 atgccggtgg tgtgtagggc ataaaagcca tgagtacagt taccaatgca caaataactc
     1621 caatgccaag atatgcattc caaattcccc aacggtctga aataaaacct cctgctatgc
     1681 aacccacatc acttacaatg cccgataaga ttccggtgac aagagcaacc gtatctgcat
     1741 ctgctttcca gtcctgagcc actgctgacc atagattgga catggcaccg gtgccaatgg
     1801 gcatcagtat aagaatcatt acaaataaag ccacgggcaa ttttatcata gaaaaaatgt
     1861 ttatccccat tcctttaatc tcaagaataa ttgatttttg ttttttgaag ggaatatctt
     1921 tgatgagaaa cattgttaat gcaaacaaaa cagacaccat acataaaaca ataccagcaa
     1981 gggtaagatt atagtgtgtg gcaagccata ttcctgctcc acctcccaaa cctgaaccgg
     2041 caaggctacc accctgatgc ttcacctttt ttattttcgt gaatttgttt tgccataatc
     2101 ccattaagag gtaatagagc tatagtggca gctacttgcg atataaagac aattactgaa
     2161 agcaatgttt ctccttttat tgtaaacgga attgaacaaa taagcaataa tgaagcaatg
     2221 gaagctgaca tgcttagcca ataccatttg tgtaagctaa gagataaatc tactattggc
     2281 ccccaaacaa aacgccatag gtttgctgaa attccgatag caacgatacc tgcagttagt
     2341 gcaactgaaa agccattctg tgtgagcata aatggtaatg tgaccgttat aaagccctgg
     2401 cttataccca ttggcatgat tagaaaaaat atataaaatg gttttgtgta gtgttggggc
     2461 atgtggttga attttaaatt ttattcagtc tcttaatttt tgatttcaga tattaatttt
     2521 tgtgagtcta ttaagacaac aataaaagtg caacattaaa aatcaagtgc taatgaaatt
     2581 aatttcaaca ttagtttatg caaccctaaa attagttttt gaactatttg atattacatt
     2641 ttagtaagtt aataattatt gcaaccactt tattctacca caaaaaaagg ctgcctttgt
     2701 gagacagcct tttgatttga tttatgctta gtgcaaaacc gttattgtat aattagtttt
     2761 tgtgttgcct gcattgtgtt gcctgcaatt tgcaacatgt agattccttt tgcaaggtgt
     2821 gaaacatcgg cagagaaggt gttgttgcca actgctacgt tgtatgtttg ctgcatcaca
     2881 actttaccac taaggtcggt gatgatgcat tgaacagctt gctgattgcc agcattgagg
     2941 ttgatgttaa ctgttccgct tgcaggattt ggatagatgt ttaacgctgc aacatcgtcc
     3001 tgaccttgct gacggcaacc taggtttaca gtgtatgatt tagcaccact gccacaggca
     3061 ttagaagctg taaccactac aacaccgttt gtatttcccc aatcaagaat aagttggttt
     3121 gtgtttccac caccgctatt gtaggttgct gctgccggag tataagacca tgttaatgta
     3181 taggcaccgg ttgtgtttga agtgttggca ttaaattcaa caccggtagt gttggcgcag
     3241 ataaccgctg gattgcttgt aagtgctacc ggagagttgg gtgcaccttt tacagttaca
     3301 caacgatagg cactgctacc gcagctattg gatgcagaaa cgcaaacact tcctgtagtt
     3361 atattgctga actgaatgtt tacactgttt gaaccattgt tgttaattgt gcccggtgca
     3421 gtccagttat aagtatttcc ggcttgcaat ggacaactga agtttccact ttggttacac
     3481 aaaccatttg ttccgcctgt gattgaagca ggaactgaag gaagacccgg agcaacagtt
     3541 ttacatttag gcgctgtagt tacaccacaa atgctggtag cagaaacaca aatatttccg
     3601 acaccattgt aacttccatt aaatgttacc tgcacattgt ttgtaccact acctaaagtt
     3661 actgatgagt ttggaggcgt tgtccagttg taggtttgaa tgcctgcaag cggagcaaca
     3721 ctaaatgaca ctacaccgtt aggacatgca gtagcgctgc cactgatggc accaactgca
     3781 ggagtagaag aagtaatagt catacatttt gatgggcttg tgaaacaacc tacttgtgat
     3841 gaaacacaaa gtgtaccgcc tgtccatgcc ggaccaaagt ttacagttac gctgttggta
     3901 gttcctgaaa ctgtggcatc gccagtgata gaccattgat aggattctgc accggcaacg
     3961 gcagtacaag tgtaagtatc gctggtggtg gcacaagcta ctttgtttcc actaacagta
     4021 gcaggtgttg aaactgatct gcgtgtaaat gcagaacgat agctgcttgt tccgcaggca
     4081 ttgcttgctt caacacgaac agagtagctg ctgttggctg ttacaccgta ttgcacatcc
     4141 attgagttgc tgccattggc agataaaaat gttattcctg tgttggcacc aacaacatac
     4201 cagttgtagc tattggcatt agttgaagat gttgttaaag tataaggacc tgcaggagga
     4261 caaacacttg ttggcgctcc tgaaacaact gcaggttgtg aaggcacacc tgtttgaatg
     4321 gtaattgtta cctgatcgtt aactgcacta cacaacacac tgcctgttga agtgagtgta
     4381 agcactacac tgccattgct gatgtctgtt gttccgggaa cataagcagc atttaatgtt
     4441 gttgcatttg gagagaaagt tccatcgcca gcagttgtcc atgttccacc ggtagcatcg
     4501 ccaccgatta taccatttaa tgctgcactg ccgctggagc aaataatatc atcgcttcct
     4561 gcattggcag taggtgctgc ttctacggtt gcagatacat tgtctgttac agtacatcca
     4621 ttatcatcag ttacggttac tgtataagtt cctgcaagcg gattgcttac agaaggattt
     4681 tgtgtcatgt tgctgtagcc accgggacct gaccactcat agccggtgat agttccatcg
     4741 gaaccacttg cattggcatt aaggttaagg ttggtgcttg aacagattgg tgcattgctt
     4801 gatgctgtta cggtaaacaa atcgcaaaca tctaaggtag taaggttaat gacattgctg
     4861 ttgtctgaag tagctacacc atcagtagca cgaacacggt agtagtaagt tgtgatagga
     4921 ttcagacctg ttacatcata atataaacct gcaacgcttt ggttttctaa aacaaaatca
     4981 ccttcgtagc cggtaacaaa taaatcatca atacggttgg tgccggtagc tgcaaccgaa
     5041 ccaccaccaa cagaagtatt gcttgtcata atccaacgca gatacacact gctttggttg
     5101 tcgcatgctg caggtaaggc aatatctgtt aatacaccgc tagtaaaatt attagctaca
     5161 gtaactgaac tgcctgttac atctgtccat gtgccaccac tgccaatttt gtattgcact
     5221 ttaaaatcgc gaggaccggt atttgaagat tgttgtttgc ttgaaaatgc aaggttataa
     5281 tatcctgttg catcaaaact tacttcccaa tatttggtgc cgttaccatt atcccaattg
     5341 tttatactgg cactttttgt tgaagctccg ttagtagaaa acgtcatagt gccaccggca
     5401 gtagaagaaa ttgttgcaga caggttactt aatattccac catcggcagt agcatcatcg
     5461 ggatcatttg gaaaatccca atacaccaaa tcagttgtaa caggagccaa aaatgttgaa
     5521 gaagtagaaa catctaaaaa ataacctgtg gcaccggaaa cactgttcca gttggcacgg
     5581 aaagaatgaa tgccaatgtt ggtagctgct gttgctacgg gtgcagtaaa ggcatctgtt
     5641 gtgaatgatg cttgtgcacc atagcctgtg cctgcagtgt tggttgcata ggcacgatag
     5701 tagtatgttg tgttgcccgc aagacctgtt agtgcagaag tgtaggtgtt gccactgatg
     5761 ttgctgctgc taacctgtgt gcctgttccg ggagtaaagt tgtttgtagt gctatattca
     5821 atgccatagg cagaaacgct agtacatccg ttatcggtaa tagttccttc gcaggtggca
     5881 gtagtttgtg tgatgcctgt tgcactgcct gtggtgagtg aaggtgaagt attaatacct
     5941 gatgcagatg aaacagaaca actgccggca gatgcgccac caccggttac aggaatagag
     6001 cctgcatagc tttgaacgag cgttggcgaa aatttaacat aaacagtagt gctgcttaat
     6061 gtaggtgccg tatagggcag attgagtgtt gaagtatatg ttccaccggg agtaaggcag
     6121 tatgtaaatc cgcttaatgt accaacatct acgcttgaac catcgaggtc tgtaccggaa
     6181 acagtaaatg tttcgtagtt tgttgtagta ccaatacaaa cattgccgaa ttgattagag
     6241 agtgtgccga cagtgagtac agggctgcca ccatcaggat caacagttcc tccaattgca
     6301 agatcattac ctgtcagcct tcctagcgca aaagtgcctg ttcctgcaga tgccccataa
     6361 ccataaagtc taatagtaat agttgttgca ttacttacgt tttgtaaagc agaaattaca
     6421 cttaaatcta tttgactttg cgcagttcca tttgttggac tggttgtata actaattgca
     6481 gaaccaatat taatacctgc tgttgcaaaa ccatccaaac tatattgcca ttggaatgtg
     6541 ttaggacctg tattggatct tctgaaatta acatctaatg ttgaaagaga cactttgtag
     6601 ccagattttg cattaactga aaactgctga tattcgttgt ttgtaactgc attagcaaaa
     6661 gttccgctta cggtgtaatc ggttgaagca aaagcattag taagtccaga agcattaaca
     6721 ccgctacctc ttgaaactga tgaactgttc aaattagcat caagtgtggt ggctgcaatg
     6781 gtagtttcat ttcctgctgc tccattaaat tcccatgcta aaatctgtgc attggcacct
     6841 agggtaaaag tcagcaaaca aaatgctgca agggcaatac tttttacacc cttcgaaaag
     6901 aataaatttt taaacataat atttggtttt aatgatttac aaaataagtt ctttgttgct
     6961 attggcaacg ttgcaaaaat aaaacaacgg ctttaattga aaaatttagt ttttaacatt
     7021 ttttaagtgc cgcaacatca cactcttatg cccacaacca atacatcgtc aacctgttcg
     7081 tgattgcctt tccatgaatt aaaaagctgt tcaagctgtt ctttctgttt gtccataggc
     7141 aggtgctgca taacctgcag gtattgctta aaccttttac tcatcatttt tttaccctgt
     7201 tcgccaccaa actgatcgga atagccatca gaaaaaatat aaatggtgtc gttaggcaat
     7261 aaatctatgc tgtgtgcagt gtatttgttt ttatggtcaa cctgcatgcc tccaatcgga
     7321 aatttatcgg gtttaattac ttttatttct tcatttctga aaagccaaag cggacggttg
     7381 gcacctgcaa aaagcaattt attttcttta aaatctatgc tgcacaagcc aatgtccatg
     7441 ccatcgttag aatcgccctg ccgttgtttc aaagagttga tgatgccttc attcagttcg
     7501 tctaaaataa ctgcagggtt ggtaatgttt ttttcgttta ccagatggtt tattaacgaa
     7561 gtaccaatca tactcataaa agcgcctgcc acaccatgtc cggtacagtc ggcagcagct
     7621 actattacct tgtctttgtt ttgcaataca ctgtaaaaat ctccgcttac aatatcttta
     7681 ggtaagtaaa gtataaagtg atttttcagt aaagctgcaa tgtttttttg attaggtaaa
     7741 acagcagcct gaatgtgtcg tgcatagtgg agactttgcg taatttcttt atttttttct
     7801 tctacgagta acttttgttt ttcgatgaga tggttgtctt ttcgtttacg cacaaagctt
     7861 ctgtagctaa cggcagcaag ggcaagcatc aatgcaaatc cggcaataaa actgtttctg
     7921 agtaattttt gtttctgcac ttcctgttgt gcaacttcat ctttcttttc ctgaacagca
     7981 cgggcaatgg catctttttt atcatactca tattgcattt gtaactgtac ggttttgagt
     8041 gtgttttctt cattaacaat gctgtcgcga tagcggataa acattttgta gtgttgcagt
     8101 gcatcgctgt attttcccaa agcgctgtcg gcctgactga gtccttcgta actgtttttt
     8161 ataacttcaa cttcgccaat ttctttacca atctccaatg ctttctgaag ccattcagtt
     8221 gctgcctgtg gttgtttcat ctgcaccatg acattgccgg tgttgatgct tgcagcagca
     8281 attgaagaaa ggtcgcctgc ctcttcagca atgcgtaggg catcagtatt gtgttgcaag
     8341 gcattttcat agtcgcccat ttcaaaatag gctgcaccca tattgttgag ggctgttgtc
     8401 atacctactt tatcaccggt ttcttctgct attttaagtg cttcggtata gttcttcagc
     8461 gcttcggtgt tgttgccttg tgcctgatag acattgccta tgtttccata ggatgaggca
     8521 atacctcttt tgttgcccaa agacaatctt atggctaagg ctgcacgatg attttttaat
     8581 gcttcgtcat aatttttttg agcattaaac actacaccaa tgttgttgta tgaaagtgca
     8641 atgccgtttt tatcgccaat ggcctcttta atttttaaag atgcaaaatg gttctttaac
     8701 gcttctgaaa aattgccaag tgttctgtac accaaaccca tattgttata gcaagcagaa
     8761 acaccggact tatcatcaat agagctgaaa atatttaatg cccgtccgta gtaaaccaat
     8821 gcatcgctgt tgttgcccat atcatcgcag gcactgccaa tattttttag tgaaacagcc
     8881 atgcctcttt tccaattcaa cttttgcgct aagtttaatg cctgctgtgc ataatacaat
     8941 tcgctgtcat aaacagcagt ctgataatag acatagctaa gttcgttcaa tgtatttact
     9001 ttagaagtat cgtcagcttg tgtgcgaagc aaatgttgca atgaatctga ttctgcctgc
     9061 tgtgcccaaa cggagacatt caaacaaaac atgaaacata aaacaaatga aagtaaacgc
     9121 ataggtgcaa tttctgaaag gtggaaagat agaaataatt tttatgtggc aataagcagt
     9181 gttgtttaaa accaagtttg gatttcattt ttatcacatt acttaaacat tacatttgtc
     9241 gcataatatt gaactatgaa tctgctttct atacgagagg tttcaaaaat ttatgcaaac
     9301 cacaaagcat tagattcggt gagtattgaa attcccgagt cgagtgtgtt tggactttta
     9361 ggaccaaacg gtgcaggaaa aacctcgctc atacgcatca tcaatcagat aacagggcct
     9421 gatggcggca gtattttatt tggcggacaa acactgaacc cttcgcacat ttctgaaatt
     9481 ggctatctgc ccgaagaaag agggttgtat aaaaaaatgg aagtgggcga gcaggctatt
     9541 tatcttgcgc gactaaaagg tttgagccgt gcagatgctt taaaaaagtt gcgctattgg
     9601 tttgaaaaat ttgaactgca aagctggtgg cgcaaaaaag ttgaagagct aagcaaaggt
     9661 atgcagcaaa aagttcagtt tattgtgact attctgcatg aacccaaact gcttattctt
     9721 gatgaaccgt ttaccggttt tgatcctatc aatgccaacc tgattaaaga tgaattgctg
     9781 gggctgcgac agaaaggcgc taccattatt ctgtcaacac atcgcatgga aagtgtagaa
     9841 gaactgtgta cacacattgc actgattaat aaatcgaaaa aaattcttga tggacaggta
     9901 aaagatgtgc gcaaacaatt tcgttctaat ctttttgaga ttgaatacaa aggtacatct
     9961 gtaggttttg ccaatgcact atggacaggc tttgaattgg ttgaaacacg tgcacaagac
    10021 gaccatgcat ttgcagtaat aaaaaacctg aacaacagca gtacaaacga tttgttgcga
    10081 caactgctca acgaagtgga agtagtttct ttccgtgagc gcatacccac tatgaatgat
    10141 atttttattg aatcggtaca aaataacaaa cctgtagaag catgaacatc atactgctga
    10201 ttatacaacg tgaatatctg acacgtgtaa agaaaaaatc ttttataata atgaccatat
    10261 tgggtccatt gcttatgggt ggactcattg caggatttgc atacctttcc atgaatcaag
    10321 gagactccat atcaatgatt cgtgtggtgg acgaaacagg agtgcttgcc ggtaaactca
    10381 ataattcaaa aacagtaatc tttactaaag acaccatgcc cattcaaacg gcaaggcatt
    10441 tgtttaattc aaatactgat tatggtatcc tttacttacc tgccaacgcc ttgcagaagc
    10501 ctgatgcaac agtgctttat acagagaagc aacctaacat ttctgtagtt gaatatttgg
    10561 aaagtgagtt gagtaagaaa attgaaaaca tgaaactgac cgatgcaggt attcaaaaat
    10621 caacactcga ttctataaaa acagacatca gtcttcagca aaaacccgtt gacccgagtg
    10681 gaaaagacaa atcattttct gcaggtgttt ctgccggaat tggctttggt gcaggactgc
    10741 tgatttatat gtttattttc atgtatggca tacaggtgat gcgtggtgtg atagaagaaa
    10801 aaaccagccg cattgttgaa gttatcatat catcagtaaa accatttcag ttgatgatgg
    10861 gaaaaattat aggtattgca ttggtgggat tgacgcagtt tctactttgg gtagttctta
    10921 caatggcaat ttcaggtact gccacatcat tgatagtagg taaagatcag agtaaactga
    10981 tggagatgca acaaaaacaa atggtgccaa atgcaaatat tccgcaggaa aaaatgcaac
    11041 ctgccagtat ggattttatg aaaatatttg aaggatttaa catcacaaaa attctgttgt
    11101 gttttgtatt ttatttctta ggcggatatt tactttacag tgccttgttt gctgccattg
    11161 gtgctgcagt agatgcagaa acagacacgc aacaatttat gttgccgatt accattccac
    11221 tcatatttgc ttatgtagtt tcgactattg tgatgaccaa tcccgaaagt gaattgggct
    11281 attggttttc aataatacca ttcacatcgc ccattgtaat gatggtgcgt atgccatttg
    11341 acccacctat aattgatatt gtaatttcga tggtgttgtt agtggcaggt tttattttca
    11401 ctacgtggat agctgcaaaa atttaccgta caggaatatt gatgtatggc aaaaagccta
    11461 cctacaaaga attgtttcgc tggttgtttt ataagtaatg tcaatgaaca ttgctgtaat
    11521 tgactgtggc accaacacct tccatctgat tattgccggt atttcagaag gcagttttaa
    11581 aattctttac cgcaaaaact tagctgtaaa acttggcgaa ggcggcatca gcaataataa
    11641 aatagcacgt gcagccttcg gtcgtggcat tgacgcactt aaagaatttg ctgccgacat
    11701 gaaacaattt atcattgaca aacaattgtg ttatgcaact gaagccatac gcagagcagc
    11761 caatggaaaa gattttgttg aagaagcaaa aaaacaatca ggcattacac ttgaaattat
    11821 tgatggcttg cgcgaagcag aactgatata ctcaggagta agtcatgctg ttccttttgg
    11881 aaaagaaaaa gtactgataa tggatattgg tggaggaagt acagaatttg taattgcagg
    11941 aaacggaaaa atgttgtggc ggaaaagttt tcctttgggt gctgccctgt tgttagatat
    12001 gtttaaacca tcagacccga ttcatgaagt gcaggaaaaa ataattactg aacatatcaa
    12061 ctcagtttta tttccactta cagaagcatt aaaacaatat ggtccggtaa atacattggt
    12121 aggatcggca ggttctttcg atacctttct ggatttatgt cttcattgtt ttcataaaat
    12181 aaaagcaaac actacctggt ggccattgga ctcagaggaa tattttgatg tacatcaaat
    12241 tttgttaacc tcaaccttac aacaacgact tgaaatgccg ggaatgattg ccatgcgtgc
    12301 cgatatgatt gttgttgcat ctatgttact tcactatgtt attcacaaac acaatatgaa
    12361 taaactttta gtgagtgctt atgcattaaa ggaaggaatg ctttattttg caatgaataa
    12421 ataacaacag caaactgacc gtaaacaaaa gagcatgtca cgtatattaa ttatagatga
    12481 tgagaaaagc atccgccatt cgctgaaaga gattcttgaa tacgagaatt acaaagtgga
    12541 tgaagcgccc gatggtattg aaggcattaa gatggctgag aaagaaaaat atgatattat
    12601 tttttgtgac ataaaaatgc cacgcatgga tggcattgaa gtgttggaga atttacaaaa
    12661 taaatgtcct gaagtgcctg tggtgatgat atcgggacat ggcaacattg acactgctgt
    12721 tgaagccatt cgtaaaggtg cttttgattt tattgcaaag ccgcttgacc taaatcgtat
    12781 tctcattact gttcgcaatg cactcgaccg tacagtactg gtaaaagaaa ccaagacact
    12841 gaaaagaaaa atttcgcgta ccaatgaaat gattggtgaa agtgaaggca ttcgtaaaat
    12901 aaaagacatg attgaccgtg tggcacctac cgatgcacgc gtgatgatta caggaagcaa
    12961 tggaacagga aaagaattgg tggcacgatg gatacatgaa aagagcagca gaaacaacat
    13021 gccactgata gaagttaatt gcgcagcaat accatcagag ctaatagaaa gcgaattgtt
    13081 tggtcatgaa aaaggcgcat ttacctctgc cataaaaaca cgaagcggaa aatttgaaca
    13141 agcaaacggt ggcactattt ttcttgatga gattggcgac atgagcctgt cggcacaagc
    13201 caaagtgttg agagcattgc aggaaaacag aattacaaga gtaggtggcg aaaaagattt
    13261 tccggttgat gtacgtatta ttgctgctac caacaaagat ttaaaatcag aaatagaaaa
    13321 aaataatttc agagaagatt tatatcatcg tttgagtgtt atactcattc atgtgcctgc
    13381 attggacgac cgcagagaag atattccggt tctgacaaat tattttctga aagaaattgc
    13441 tgatgaatat aaaagccctg caaaagaaat tgctccggat gcaatagagc agttgcagaa
    13501 aaaaaactgg acaggaaata ttcgtgagct gcgtaatgtg gtagagcgtt tggtaattct
    13561 cggtggcaaa accatcacta aggatgatgt tataaactat gtaggcgtga aataattcac
    13621 gcccacataa ttcatttatc aattctgatt acaacctact ttgtcaactc gacacaggct
    13681 ttagtactac caaggtacta gtgaaacacc aatagtccag ggatgcaact ccggcccctt
    13741 tccactcttg aaaagttctg acagtgcata gtttgcaaat acagtaaaat cactgtagcc
    13801 tatgcgaact gttgcatcat aacggaaggg atcaatatta aaatcatcaa aagttttatc
    13861 cttctctttt ttaccatctt tattgtaaac ctttttagta tgtgagccca atttgtaact
    13921 aaacaacaaa ccggtagtaa tgtgaaaagc ttttttaaga tttgaacttg tgttgaaagt
    13981 aagcagaact ggaacagtgg cataacttac agtaagttta ttcttacgca ggttcatggt
    14041 gtcctgagta taaagcaatg cattggtatc aggaatcagg ctcatatttt ttctgaaacg
    14101 ataattattg tatgataaac caatacctgt tgaagcaatg acataacgct taatcagttt
    14161 tacatcgtac tcataaaagt tgatgtttac tgctaaggac ttttcaggaa tcatctctaa
    14221 aaaatcatac ccatcaggag tatcgaattt tgatttactg ttataaagtc cattaaatcc
    14281 caattcaaat cccgaccaat aagtaccgga tttattgctt ccagaaattg taatactgtt
    14341 tgagttcagt tccgattcaa ttgcagaagt tataggttca atttttccgg agcctgcaat
    14401 ggcctgatta attgttcttg gcgaagaaac ataatacaga tttcctgagc cagcaatgtt
    14461 ggctgataat gaatcgctaa cccaaactga tgatgtgcca ctgccgctga catttacttc
    14521 tgcccgatca acatttaatt tatgcgtaat aatttttcct gagccggcaa gatttgcatt
    14581 taatgattgt gcagaacctc caagaattat ttttcctgaa ccacgcatgt ttgcctcaat
    14641 gtgctttgct tttacatagt tcatcttaaa ttttccacta cctgaaatat ctatattcaa
    14701 gtcgtgcaat tccatagtat cttctgcaga aatacttccc gaaccggaaa tatttatctt
    14761 ttgcaaatca ggacttctga caattatatt acttcctgca ccacttagca caagggtttt
    14821 attttcaaga ctccactgtg tggtgacatt atcttcaggc acttcaattg accatgtact
    14881 gtccaattta taaactgcat taagcgatcc acgcaagtct atgtctgtaa actgatctaa
    14941 agttttatgt ttttgtgcat ttgcaaaaag tgcaaacaac attaaaaaca aggtcagact
    15001 aaattgatta catcttttca tatccttcat tttattgtat gacgcaggcc tgttactaaa
    15061 agttacagca ggtgcttata taactaaact acctgctgta aaattaaatt tcattgttat
    15121 ttactccagt acaacacgtt gcactttaac ctgatcgtta gcaacaactt taaccaaata
    15181 aactcccttt ggttgattct ccagatcgaa agttattatt cctgcattaa aatttgttct
    15241 gttttgaaca gccacacgct gacctaaagg attataaaca gacacctcta acgggcttgc
    15301 cgaagttaat gtaatatctg cattgatgat tcctttggtt ggattaggat aaactactat
    15361 atcagcatag cgctcctgat tgttgatgcc aacaacggca cccggagcag aaacaaaacg
    15421 atagctgtaa cgtgagccaa aatctctttc gaaatttctt gcaatgctat tgttcaatcg
    15481 cagaatacga tggtaacctg ttccctgtgc tgagtttgcc caccagtata aaccatcatc
    15541 gccactatct gttatttcat aacgataaca accgggagtt gcatgaatgg tgtcacgata
    15601 aattgtgttg gcagccattg agcccggtag tcgttgtacg atgatattat tattttcatc
    15661 ataaagttta tagccatttt ccgtaggata gttatttgtt tttaccaata agataaatac
    15721 agtgtccatc acttcaggaa cataggcatt tgaataagct gtatcatcat aaacatactg
    15781 atctgccgta ttgttaacgt cagcaatgta tgctttaaaa attctgttgc cacttgtata
    15841 gctgattaaa ccgggtaagt caacatctgc tgttgcaaat gtatttattg agcctgtcca
    15901 gttgaatgta ctttcgggtt cgcctactaa tccatatttt attgttgcag aagttattgc
    15961 agttgagcca ttgtttttta ttctgatttt tggattgttg caggtagggt tgctgcgcga
    16021 aaattcaaac ttatcggtag gtgcaagaat ttcatctatg gcagcatcta tattgttgtt
    16081 caatggacca aattcaaaaa gctgtccttc gatgacgtaa ttagcggaag ggcttgttac
    16141 gttatagttc tgcatattca tgtcaataat gtgcgtgctt cctccggcaa cagcaaaatt
    16201 attgacagca ggatagaccc agttaccggg acaccaattt gcacggtcgt aaacccatgt
    16261 gcctccttgc ggataaatag ggtttgttcc acacaaccgc caaacttgct tggtatctac
    16321 caatgaatta tcaaaataaa tagttcttgt ttttgagcaa aattctgcac aataattggc
    16381 atcggcacca tgtccggatt gggtaacatt cagtcttaaa tttgcagtat taaaattaag
    16441 cgtcacagtg tcggcaccga gatagttttc aattggattg gatgcattgc cataaggaaa
    16501 actgccatgc cacaatcttt taaatgcaat aggattcatc accggagtac cttctatcaa
    16561 tgcaaaatca acactaacga gccatccgcg acccacattg gtttcgtagc cggtgtggat
    16621 atattctatc tctacagagt cgtgaaggaa tgatgcaaaa tctgttacat caagcgtcca
    16681 tgacgacttg aatgtagagt tgaatgtatt gccataaggt gttatataac gtgccagttc
    16741 aatatcctga gagggattgt tctgacctcc tgtacgtctg atgtaaatat aatcgagata
    16801 atcccattcg ccacatgaca tagggcttgg gcaacgatgt gttaaagtta aaattatctt
    16861 acgatagttg gtggatgcag aaggaaatac tacccaattt ggataaccat tgctacctgt
    16921 gttgggattg gttactacag tgtcattggt atgcgagctt acaaaggttg tgtcgccaac
    16981 agatgctgca caagttaaag ttgttgcaac agaaaataat atcagaaata acttcttgac
    17041 taacatgtga aatgattttg gttggtgaaa aaatgtatta caattacagt gcaattttaa
    17101 tacaaaaaat ccgaatattg gttgttggag tgcaaacatt ttttagaaac tgcaccaaca
    17161 aatcggtgtt aacaacaaat tgtcatcgtt atgtatatct ttgcttacta agcttgctgt
    17221 tcttattttt gtttgcgtta aaattaatat gaaaaaattc atttcaggtt tttcaattct
    17281 gtttcttttc tgtagtcttc atgtatcagg cggtggtttt cagatgcaac cacaaagcca
    17341 tagttcagca ggtatggccg gtgcagtgat tgcattgcaa actgatgggt cagttatatt
    17401 ttacaatccc ggtggcatgg tttttcttga gaaaagtaat gtctttggtg gtatttctta
    17461 ttcgcagtca agaatggctt tccgtagtaa caacagcgga ttggtaaacc gtacagaatc
    17521 aaaaaatatg ctgccgtttt atgtaggtgg caccataatg ttatctgaaa aaatggctgc
    17581 aggtattgta gtcaatacgc cttttggata caacacaacc tggaaaagcg attgggaagg
    17641 gcgaaatatt atactgtcag ctaagctgaa caccatattt attcaaccta cattctctta
    17701 taaaatttct gaaaaattag gaattggtgc aggctttgtt tttgccagtg ccaactataa
    17761 atggaatcgt gcgttgatag ttgcagatgg agcagatgca caacttgatg gtaaggcaac
    17821 aggctttggt tttaatgcag gaataaatta tgccatcaat aaaaaattaa ctgctgcact
    17881 tgcataccgg tcaaaagtta aactcaactt taaaaacact gatgttacct ttagtaatat
    17941 tccggcatcg ttaacgaatc aatatcctgc ctcagcaaat gccgctgcag aaatgccgct
    18001 gccttctgtt ttgtcaattg gtttcagcta tgaagtcatt aaaggattaa aacttagttt
    18061 tgaatctaac ctcaacggtt ggtcgtcatt cgattctctg aattatgaat ttgatgcacc
    18121 aaacacagca ttaaatcaag aatttcgtca ggcacgcaaa tacagagatg cattaacact
    18181 tagattaggt gcgaattata aaatcagcga taaagttaca ggtcgtgcag gtattgcctt
    18241 cgatcagact ccggtaaaag aaaactacat gacccctgat atgcccgatg gcgacagata
    18301 tgtttttggt attggtgcat catttaacgt gagtaaaact tttgttattg aagggtcata
    18361 caattttgaa actaaggccg agcgcgaagg tgtaaataaa gataccggat tgaatggtgc
    18421 attcaatgca ctgtcgcatt gttttggtgt tggtgtcaac tattcatttt aattaaataa
    18481 taaaccacaa tataaacgca tgagcaataa tcatattctg aaaagtttac ttcttgcagt
    18541 aatcatgatt gcaggttgca agcctgagtt gaaagagctg aagttcgaca acaacagtat
    18601 tgacttttcg aagtatgttg ctattggcag ttcttacaca gcgggctatc agaacaatgc
    18661 attgtttgac atggggcaac aaaatagttt tgcagccata cttgcctcac aattaaaaaa
    18721 agttggcggt ggcgagtttc tgcaaccatt ggttagcgga aatgatggtg cagggttcaa
    18781 cttttttctt cagacctata ttccacagac aaaacttggc tatggcacaa catgcagttc
    18841 tgctccgggc ttctctacat ttccatccaa cgtaactgac attgtcgcag tagaaactta
    18901 catcggcaac aatgcacctt acaacaacat gggtgtgccg ggtatgaaat cgtctgattt
    18961 agacaatgcc aattttggct ctacagattt actgctttca aaaaatcctt tctacgcacg
    19021 atttgctacc aaccctgaaa catcaacagt acttacagat gcagtcagac aactacccac
    19081 gttcttttct ttttgggttg gctttaacga catttatgat tgtgcaatgg caggcagcaa
    19141 tcagacattg gctaccatta caccattagc taattttcaa aataacatca ataatgctct
    19201 tacatcactc attgcaggaa atcgcaaagg tgtagttgca aatattcctg acctactcga
    19261 cctaccctat tttacttcca tagcct
//
LOCUS       DAOOOI010000043        18359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_614_length_18359_cov_16.426901, whole genome shotgun sequence.
ACCESSION   DAOOOI010000043 DAOOOI010000000
VERSION     DAOOOI010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 18359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18359
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_614_length_18359_cov_16.426901"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="PKX59_06750"
     CDS             <1..676
                     /locus_tag="PKX59_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009389904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOZ90603.1"
                     /translation="EGKKEQSMIEQLAIRSMAKAVYSTENIGHYGLAFDFYTHFTSPI
                     RRYPDVMVHRLLDYYMSGGKSVSQQEYEEKCRHCTEREINAADAERSSIKYKQVQYLA
                     ARKDEIFKGVISGVTEWGLYVELEDNKCEGMIRLRDLNDDYYEFDQKNFCIVGHRSRK
                     KYSLGDELMVVIKSTDIIKKQVDFMLYDANRLHEVSPFKRSDKKRFPTSGKFRGDKKK
                     GGKRRR"
     gene            748..1794
                     /locus_tag="PKX59_06755"
     CDS             748..1794
                     /locus_tag="PKX59_06755"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOZ90604.1"
                     /translation="MLKKILITAWIISMATGLQAQQKKSYQVGVIAFYNLENLFDTIN
                     QPEVNDEEFTPTGSNNYTGKVYLDKLGKLSDVLSLIGKDESPDGFSVLGCAEVENESV
                     LKDLVNEPKLKSRNLKIVHYDSPDKRGVDVALLYNPKYFTPKFSEPLFIDLRTPENPE
                     YYTRDILYVYGLYLGEPVHFLVGHWPSRRGGEEASAPSRAKAAAVCKNKIDSITKINA
                     DAKIVLMGDLNDDPLSPSVTKVIKAVGSKDKVERGGMYNPWVDFYTKGIGTLAYNDAW
                     NLFDQIMISSGWLNKKQDGFFFKDAHIFKREFMLQKSGRYKGYPLRTFDFSRYIGGYS
                     DHFPTYLVFLKEVK"
     gene            1819..2562
                     /locus_tag="PKX59_06760"
     CDS             1819..2562
                     /locus_tag="PKX59_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ90605.1"
                     /translation="MKGTLFEIGRYFGFIKNLFYKPEKLSIYVRQVFREMVNLGIGSL
                     WLVALTSAFMGAVITIQSAYNLVNPMVPLWAVGIVARDSIIIEFSPTIIMLVLAGKVG
                     SSVASEIGTMRVTEQIDALEIMGINSSGYLVLPKIIAALLICPVVVIISMIVGITGGW
                     VAGDLSGVVPSADYLKGIQNQFLPFNVTFAMIKTVVFAFIITSVPAYHGYYTSGGALE
                     VGQSSTKGVVYSSILILIFDYLLTQLILA"
     gene            2559..3311
                     /locus_tag="PKX59_06765"
     CDS             2559..3311
                     /locus_tag="PKX59_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ90606.1"
                     /translation="MIAIENISKSFGNKKVLEDVSCEFDKGKINLIIGGSGQGKTVLM
                     KCAVGLFEVDKGKVLYDGRNFSEMTFGERKPIRQEIGMLFQGGALFDSLTVEQNVTFP
                     LKMFTNMTNAEKRDRANFCLHRVNLENVNHLLPSAISGGMKKRVAIARAIAMNPRYLF
                     CDEPNSGLDPKTSILIDELIKEITEEYDITTVINTHDMNTVMSMGEKIIFIYKGKKFW
                     EGNNEDIMRSENKELNDFVFASRWMQAVKKHF"
     gene            complement(3320..4618)
                     /locus_tag="PKX59_06770"
     CDS             complement(3320..4618)
                     /locus_tag="PKX59_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90607.1"
                     /translation="MRKIFISILFIISAGITNAQIDNLSNMSPEWIRTGGRNAAIKGS
                     DLVVYNPATLTRLEKGLHIGLGNQMFMRKPSHTYDLGWGEVKHEQDGNDYFVPNLFLS
                     YNKNKWAVWGGVYISGGGATANYPEGSLNTELIGLSLLSAFQGAYLGTGQQYLKASSL
                     YLTGTAGFSYAINEQFSAALGVRYVNATNKTKAGTELIGDFSNLPVNMEQEDNANGIA
                     AVISLSARPIEKLNIAVRLETNTKLNFKTKQKVDDLEQSTGGMAELVVDGQESRRDLP
                     GLAGIGIGYEFSKAFRLTAEMNYYFQQHADWGKRADGKRINTLAGDAMNFGGGFEWMW
                     SKYITWSAGAVYTMLDFNDMAGYYTTLGAFETAPYNNLTANTGFAIDLSEKFRVNVGV
                     AKAFYKKDKTVAIEAMAPNVVKAKLNNDVLMLGAGIDLRF"
     gene            complement(4713..5297)
                     /locus_tag="PKX59_06775"
     CDS             complement(4713..5297)
                     /locus_tag="PKX59_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HOZ90608.1"
                     /translation="MKLSIDLTYNTTQAWVDAVMNDFDSFLQDHADCERKASAMAMSF
                     VSKCPDKTKIIPELIETALEELKHFQQVYHIMEKRGIQLKAEMPEDLYIKQLLALLRS
                     KSSERLMDRLLLASIIECRGTERFRLVEEALQDNELKRFYKMLWASEAKHGNIFVKMA
                     LEYYPKDDVYKRLHELTVKEGEICKALLIKAALH"
     gene            5313..6584
                     /locus_tag="PKX59_06780"
     CDS             5313..6584
                     /locus_tag="PKX59_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90609.1"
                     /translation="MYKSNFPLNKPHTYFEKQQFVPLLQEPPHSQLQCIVVIPCFNEP
                     DLITTLDSLWKCDETKFPVEIIAVINSGRNADSNVMDCNNLTLKTMQLWMQKHNSNHR
                     HYQVIHCSDLPPKKAGVGLARKIGMDEAAYRFSLNGNDNGIIVCLDADCTVQPNYLVE
                     IEKHFSNNIKTAGCSVYFEHPLEGKQEPSIYDAIINYELYLRYYVGALRYCGFHAPYH
                     TIGSSMAVTNKAYQKQGGMNQRKAGEDFYFLHKIFHLGNFSELNTTTVYPSPRISNRV
                     PFGTGSAISKIVTAGNNNYPVYAFETFEILKVFFDQIKVNATSREMINIDSFAPALKN
                     FLSAHNAASKMIEIATQTINSKTYLQRFAHWFDGFMVLKCVHYMRDHFLEQEEVLVAT
                     KKLMNNLNISPGNLTKREMLLRLRKHDRSGS"
     gene            6605..8044
                     /locus_tag="PKX59_06785"
     CDS             6605..8044
                     /locus_tag="PKX59_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOZ90610.1"
                     /translation="MKLLNYINGQMVEPISQKWMDNTDPSTNTVFSQIPDSDERDVQM
                     AVNAAKQAFVGWSTMPVKKRSDLLLRIAALIEKNLQRFAEAESLDNGKPVWLAKQVDI
                     PRASANFHFYATAALHLAAETHAMENTAINYTLRQPIGIAGCISPWNLPLYLFTWKIA
                     PALASGNCVVAKPSEITPVTAFLLSQFCIEAGLPAGVLNIIHGTGPRVGSAIVSHPDI
                     PVISFTGGTKTGAEISRIAAPMFKKLSLELGGKNPNIIFEDCDFDHAVSTSIKSSFSN
                     QGEICLCGSRIFIQRSIYDKFKEAFISKVKGLKTGDPKDESNFLGAIVSQPHFEKILS
                     YIDIAKQEGGVILTGGKAIQPEGRCKDGFFIEPTVIENLPYNCRTNQEEIFGPVVTLT
                     PFDTEEEVLMMANSTNYGLAATVWTTNLTKAHKVAAALHSGIVWINCWLLRDLRTPFG
                     GVKQSGIGREGGFEALKFFTEEKNICIKL"
     gene            8066..8416
                     /locus_tag="PKX59_06790"
     CDS             8066..8416
                     /locus_tag="PKX59_06790"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOZ90611.1"
                     /translation="MFITVLKSKIHRAKVTQAELHYVGSITLDENLMDAADLIENEQV
                     QVLNINNGERFVTYVIKGERNSGVVCLNGPAARRVQLGDIVIVASYAMMEKEEAKNFK
                     PVLVYPDEKNQPVK"
     gene            8430..9443
                     /locus_tag="PKX59_06795"
     CDS             8430..9443
                     /locus_tag="PKX59_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOZ90612.1"
                     /translation="MKKKVVSVIKYALFLAAGVGMLWLTFRNQDFTQVIQKIENVHIG
                     WLLASVAAAILAHISRAIRWNMLITPLGYKPKLINTFWALCAGYFANLAVPRLGEITR
                     CGMLTKAEQVPFNELFGTVIVERIMDVLMLFLLLLAVATLEFHLIGGFLYDNIAAPLM
                     SKFQNLNLPLVIGVLVVGIIAVFAYFYFSKHSGKSKAIFEKINLLLKGVADGIKSIAK
                     MKNKSLFIFHTLFIWVMYFFASYLCFFALDSTSMLDAKAGLFILVIGGLGMSAPVQGG
                     IGAFHFIVSRGLMLYGISETEGIVYATIVHGYQTILVIALGAIGFFVLIATGKRIKAN
                     NGL"
     gene            9433..9921
                     /gene="rfaE2"
                     /locus_tag="PKX59_06800"
     CDS             9433..9921
                     /gene="rfaE2"
                     /locus_tag="PKX59_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOZ90613.1"
                     /translation="MDYNEIISKKIFNEQSLLQQLSIWRLQNQSLVFTNGCFDILHLG
                     HTQYLSQAKNYGDKLILGLNSDKSGACKKGLGRPLNNENARAFVLASLHVVDAVVLFD
                     EETPYNLIKLVQPDVLVKGADYEIEKIVGYDIVIQRGGKVITIDLTQGYSTTALLNRI
                     KQ"
     gene            9989..10408
                     /locus_tag="PKX59_06805"
     CDS             9989..10408
                     /locus_tag="PKX59_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90614.1"
                     /translation="MKQAAIKIPIVALSLLLLMGCESNHFFKSEKKLKSDIVGTWNRV
                     LFGQQNEETWIFVDGAIKIYQPNRHGDTADVGTFSIDATLASPYLKIENTSYLNQNPG
                     GDTYNHKWSIVKLDNKVLEIATDSPLGGVAQIEFIKQ"
     gene            10444..11808
                     /gene="radA"
                     /locus_tag="PKX59_06810"
     CDS             10444..11808
                     /gene="radA"
                     /locus_tag="PKX59_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOZ90615.1"
                     /translation="MAKVKSVFVCQNCGAQSPKWMGKCASCGEWNSYVEELITSGNSS
                     AQVNYKRSSKPEKITEIELHQEQRIVLPDHELNRVLGGGLVRGSLILLGGEPGIGKST
                     LLLQLAVRLKNLKTVYVSGEESGQQIRMRAERLNSDLHSQCYLLTETSLEIILQHLNA
                     LKPDMVIIDSIQTMFHSQIESSPGSVSQIRGCATELLRFAKDSGCPVFLVGHITKEGV
                     IAGPKVLEHIVDTVLQFEGDRHHIYRLLRSHKNRFGSTAEIGIYEMQGNGLAEVSDPS
                     QIMLSQRDEALSGIAIACNLEGMRTMLIETQALVSSAVYGVPQRSTTGFDQRRLNMLL
                     AVLEKRCGFKLGAKDVFLNITGGLKVDDPAIDLAVICAILSSNNDIPLEPLTCFTGEV
                     GLSGETRPVSRIEQRIKEAEKLGFKKIYFPAQNLAAFDKAMFKNIKLYPVERVDEVFS
                     LLFG"
     gene            11890..12900
                     /locus_tag="PKX59_06815"
     CDS             11890..12900
                     /locus_tag="PKX59_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90616.1"
                     /translation="MKTLISAVIAMVSFTISASAQSNIEIGMSGGATNYFGDLGNNEV
                     LQSTSTRPGGAITIRNFLNSPAKTGYQYQPLSVEARFSWHRIGYDETKSIGDKKGYQL
                     RNYGRGLSFRTDVYGVSTHVSYTLYANKRKPLYAQGAAMYFYAGVGIYYARPKADLFN
                     GSIDINNRYYFWSDGTIRDQDEKTGQGNIIKRDGKYETNLDEWRTEGQGYNSELGQKK
                     PYNLMHIGFPLGMGWRWGISKNIILSAELSYIKFITDYLDDVSDRYATYEEIKANFPE
                     EHKQDLAKYISDPSGKGTNGFEGVATSQRGNHRKTDGYAFLSLEVAYRLDFNWGKIKS
                     SF"
     gene            12955..13983
                     /locus_tag="PKX59_06820"
     CDS             12955..13983
                     /locus_tag="PKX59_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HOZ90617.1"
                     /translation="MQLTTIPFQLRFRKPFVLASGTRTHTSVVYIKAIQNEITGWGEA
                     AMPPYVKETQESVIHFVQQLHIPIINTLDDLNLFFEKLYQTSGNYFAKAAINGAMLDW
                     WTRKNSLSLSEYFGIDKNLSTPDCCYTITNGDDIKHSIKQAVDFKLLKIKAGFDGDVE
                     FIKEIAAISEKQICIDANQGWVNVNVALQKTAQLKNLNIAFIEQPLPVDDWQGMQKLK
                     QHTTFNFIADESFQTIDDLKKIMDCFHGVNIKLMKCGGVSNAFEIAKSARQLNAKLLL
                     GCMSESSCGIATAGAIQQLFDWVDLDGPLLISNNPFTGVRYQEGKIFIENNVGTGALS
                     VESTLQAL"
     gene            complement(13964..14467)
                     /locus_tag="PKX59_06825"
     CDS             complement(13964..14467)
                     /locus_tag="PKX59_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HOZ90618.1"
                     /translation="MIEIRSATADDIPFITEVYNDAILNTTATFDTEVKSIEDRMLWF
                     KNHDSSHPIIVAEDNGVLCGWASLTAWSDRCAYNGTAEVSVYVHKDHRGKGIGKALLE
                     ILILKGESKGLHYLLSRIASGNEASIHLHTIFGFRHIGVMKEVGFKFGNYVDVHLMEL
                     VFKEPAK"
     gene            14789..14959
                     /locus_tag="PKX59_06830"
     CDS             14789..14959
                     /locus_tag="PKX59_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90619.1"
                     /translation="MGINVTPKDLTNKEFMLKNQSAIQHAQAILKERQTESTRITFKA
                     APQTNIFKQSKN"
     gene            15320..15490
                     /locus_tag="PKX59_06835"
     CDS             15320..15490
                     /locus_tag="PKX59_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90620.1"
                     /translation="MGINVTPKDLTNKEFMLKNQSAIQHAQAILKERQTESTRITFKA
                     APQTNIFKQSKN"
     gene            16079..16255
                     /locus_tag="PKX59_06840"
     CDS             16079..16255
                     /locus_tag="PKX59_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90621.1"
                     /translation="MDLLISILLYLGVYATPADLQNKEFVAKNSEQINKAEMLSKDPN
                     FKCDGIVIVGSGNL"
     gene            complement(16299..17801)
                     /locus_tag="PKX59_06845"
     CDS             complement(16299..17801)
                     /locus_tag="PKX59_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90622.1"
                     /translation="MEFSEIKLLINFFAKHRHDFLGVPDVYFADKNYPELLWFYKEIS
                     KGSINNDQEAAEKLLQSTATNPAYQQLKAELEDRLVNLVFGLDPEKLMNSMLGRSSFR
                     AYIYFGAAMILRQQNASAFFSDHFFKKAADYATFTNDGMIGCLTDLMLADRMAIKNKP
                     DDFYHYRNRFKKSIKQFELEAELILMQRELDIFQVASNISQYKNAAKAKRYYLRAKKI
                     HQQLKTFNSNFYYGNIAFIYGYFSSNFELVEEALNEREAFYQNHPEYYSDFQKSFIAL
                     SRMLAYIHLRQYEKGKEEARIFLGKLSANSSDWLHALEHYFILCMHSGNFEQALNIYY
                     EATRSEYFINSNQEVKERWHYFEPYLNFILPGHFPKESINLLGFLDEISYYTTHKEDN
                     NIIIMIGQIIVMIEMGDYDKLYDRTSYLESYIKKYVDKRIYPRTYIFLNMLLTLFKNN
                     FQKTKTQKQTAVSLEKLKPVAGNKFFNTEGLEVIPYEKLWAAISEKLKRH"
     gene            complement(17912..18229)
                     /locus_tag="PKX59_06850"
     CDS             complement(17912..18229)
                     /locus_tag="PKX59_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HOZ90623.1"
                     /translation="MIGAIYIDRGYGKARQFILERMINHHVDLESLEATEINFKSRIL
                     NWSQRTKNTVAFETVEDEKSNDKLIRVRLMINGEEVSTGMDFVKKKAEQIAAERACTK
                     LEI"
BASE COUNT         5808 a         3273 c         3512 g         5766 t
ORIGIN      
        1 ggaaggtaaa aaagagcaga gtatgattga gcaattggct atacgcagta tggctaaggc
       61 tgtttattct accgaaaata taggtcacta tggattggca ttcgatttct atacacactt
      121 tacttcgccc ataaggcgct atcctgatgt gatggtgcac cgattgctgg attattatat
      181 gagtggtgga aagagtgttt cgcaacaaga gtatgaagaa aaatgccgtc actgtacgga
      241 gcgtgaaatt aatgctgccg atgcagagcg ctcttcaata aaatacaaac aggttcaata
      301 ccttgcagca cgtaaagatg aaatatttaa aggtgtaatt tccggtgtta cggaatgggg
      361 tctttatgtg gagttggagg ataataagtg tgaaggtatg ataaggctgc gcgacctgaa
      421 tgatgactat tatgaatttg atcagaaaaa cttttgcatt gtaggacatc gttcccgcaa
      481 aaaatattct ttgggcgatg agctgatggt tgtcattaag agtaccgaca tcattaaaaa
      541 acaagttgat tttatgcttt atgatgcaaa tcgtttgcat gaagtttctc cttttaaacg
      601 aagtgataaa aaaagattcc ctacatcagg caagttcagg ggcgataaaa agaagggtgg
      661 caaaagaaga agataatctt tttaaaaatt aaatttaatc atcataattt caagccacag
      721 cattgtgacg ttttttagat ttgtgccatg ttaaaaaaga tacttattac agcctggata
      781 atttccatgg ctacaggcct tcaggcacaa caaaagaaaa gctatcaggt aggtgttatt
      841 gctttctaca atttagaaaa cctgttcgat accatcaatc aacccgaagt taatgatgaa
      901 gagtttactc cgacaggttc taacaattac acaggcaaag tatatcttga taagttaggt
      961 aaactctctg atgtattatc actgattgga aaagacgagt caccagatgg attttctgtt
     1021 ttgggatgtg cagaagtaga gaatgaaagt gttttaaaag atttggttaa tgaacccaaa
     1081 ctaaaatcac gcaatctgaa aattgttcat tacgacagcc ctgacaaacg cggtgttgat
     1141 gtagcattgc tctataatcc aaaatatttt actcctaagt ttagtgagcc tttattcatt
     1201 gacttgcgta ctcctgaaaa ccccgaatac tacacccgcg acatattata tgtttatggt
     1261 ttgtatcttg gagagcctgt acattttctt gttggtcact ggccatcgcg cagagggggc
     1321 gaagaggctt ctgcaccatc acgcgctaag gctgccgctg tttgtaaaaa caaaattgat
     1381 tctataacta aaataaatgc cgatgcaaag attgtgctga tgggcgattt aaatgatgac
     1441 cctttaagtc caagtgtcac aaaagtaatt aaggcagtgg gcagtaaaga caaagtagaa
     1501 cgcggaggca tgtacaatcc atgggttgat ttttatacaa aaggaatagg tacacttgcc
     1561 tataacgatg catggaatct ttttgatcag attatgattt catcaggctg gttaaacaaa
     1621 aagcaagatg gattcttttt taaagatgcc catattttta aacgcgaatt tatgttgcaa
     1681 aagagcggac gttataaagg ctatccctta cgcacattcg acttcagcag atatattggc
     1741 ggctacagcg atcactttcc aacctatctc gtatttttaa aagaagtaaa atagatagtt
     1801 ttaatatctt agctactgat gaaaggtaca ttatttgaga ttggacgcta tttcggtttt
     1861 ataaaaaatc ttttttataa acctgaaaag ttgtcaattt atgtacgcca ggtttttcgc
     1921 gaaatggtaa acttaggcat tggttcattg tggctggtgg cacttacttc tgctttcatg
     1981 ggtgctgtta taacaattca aagtgcttac aaccttgtga acccaatggt gcctttgtgg
     2041 gcagtaggta ttgttgcccg cgatagtatc atcattgagt tttctccaac tatcataatg
     2101 cttgtacttg caggaaaagt aggtagcagt gttgcatcag aaattggcac catgcgtgtt
     2161 accgaacaga tagatgcact tgagataatg ggcataaact catcagggta tttggtgctt
     2221 cctaaaatta ttgcagcatt attaatttgt cccgtagttg taatcatcag tatgattgtt
     2281 ggtattacag gcggatgggt tgcaggtgat ctttcgggcg ttgtgccttc ggcagattat
     2341 ttaaaaggaa tacaaaacca gttcttacca ttcaatgtaa cctttgctat gataaagaca
     2401 gtagtgtttg cttttattat cacttctgtt cctgcctatc atggctatta tacaagcggt
     2461 ggcgcacttg aagttggcca gtcaagtacc aaaggtgtag tttacagcag tattttaatt
     2521 ctgatttttg attacttatt aacccaactc attcttgcat gattgccatt gaaaacatat
     2581 ccaaatcatt cggcaataaa aaagtgcttg aagatgtttc atgcgaattt gacaaaggta
     2641 aaattaacct gattattggc ggtagtggac aaggaaaaac tgtgctgatg aaatgtgctg
     2701 ttggtttatt tgaagttgat aaggggaaag ttttatacga tggcagaaat ttttcggaga
     2761 tgacttttgg cgaacgtaaa cccattcgtc aggaaattgg catgttgttt cagggtggtg
     2821 cattgtttga ttcattaacc gttgaacaaa atgtaacttt ccctttgaaa atgtttacta
     2881 acatgaccaa tgcagaaaag cgcgatcgtg ccaacttttg tctgcataga gtaaacctcg
     2941 aaaacgttaa tcatcttttg ccaagtgcca tcagtggtgg catgaaaaaa cgtgtagcca
     3001 ttgcacgcgc cattgcaatg aatccaagat atttattttg cgatgaacca aactctggcc
     3061 tcgaccctaa aacatccatt ctcattgacg aactcataaa agaaattacg gaggaatatg
     3121 acattactac tgtcatcaac acacatgaca tgaatacagt gatgagtatg ggagagaaaa
     3181 ttatttttat ctataaaggc aaaaaattct gggaaggcaa caacgaagac atcatgcgct
     3241 ctgaaaataa agaactaaac gattttgttt ttgcatcacg atggatgcag gctgttaaaa
     3301 agcactttta ataaatccgt cagaatctca aatcaatacc tgcaccaagc atcaacacat
     3361 cattgtttag ttttgcttta accacattag gtgccatagc ttcaatagct accgttttat
     3421 ctttcttata aaaggccttt gccacaccta cgttcactct gaatttttct gataagtcaa
     3481 ttgcaaaacc tgtgtttgca gtaaggttgt tgtagggtgc tgtttcaaaa gctcctaatg
     3541 tagtgtaata acctgccata tcattaaaat cgagcatggt ataaactgca cctgcactcc
     3601 aggtaatata ctttgaccac atccactcaa aaccaccacc aaaattcata gcatcgcctg
     3661 caagtgtgtt tattcttttt ccatcggcac gttttcccca gtcggcatgt tgctgaaagt
     3721 agtaattcat ttctgctgtc agacgaaatg ctttactgaa ttcgtatcct ataccaatac
     3781 cagccaaacc cggtaaatcg cgtctgcttt cttgtccgtc aactaccagc tctgccatgc
     3841 caccggttga ctgttcaagg tcatcaactt tttgtttggt tttaaaattt agcttggtgt
     3901 ttgtttccag tcttacagca atatttaatt tctctatggg tcgcgcactt aagctgataa
     3961 ctgcggcaat accgttagcg ttatcttctt gctccatatt tacgggcagg ttactgaaat
     4021 cgcctatcaa ctctgtgcct gctttagttt tgttggtggc atttacatag cgtacaccca
     4081 atgctgcaga aaactgctcg tttatagcgt aactgaaccc tgctgttcca gtaaggtaaa
     4141 gagaagatgc tttaagatat tgctgtcctg ttccaaggta ggcaccttga aaagcactga
     4201 gaagtgacag tccaataagt tctgtgttta aagagccttc aggataattg gcagtggcac
     4261 ctccaccgga aatatataca ccaccccaca cagcccattt atttttgttg tatgataaaa
     4321 ataagttagg tacaaaataa tcattaccat cctgttcgtg ttttacttca ccccaaccta
     4381 aatcataggt gtgagaaggt ttgcgcataa acatctgatt gcccaaacca atatgtagtc
     4441 ctttttccaa acgtgttaat gtagcgggat tataaacaac aagatcactg cctttgattg
     4501 cagcatttct gcctcctgta cgaatccact ccgggctcat gttgctcaaa ttatcaatct
     4561 gtgcatttgt tattcctgca ctaataataa atagaatgct gataaatatt ttcctcatac
     4621 tctgattttt tgtttgagta aaaatatttt gcatttgcat tgtcattcca tgcagaaaag
     4681 aaactaataa acaatttgtt tttaataagt gattaatgca atgctgcttt tatgagtagt
     4741 gctttacata tttcaccctc ttttacagtg agttcatgca gacgtttata tacatcatct
     4801 ttcggataat attcaagcgc catttttaca aaaatatttc cgtgtttggc ttcacttgcc
     4861 cacaacatct tgtaaaatct ttttaattca ttatcttgta gtgcttcttc taccagtctg
     4921 aaccgttctg ttccacgaca ttcaataatt gaggctaaga gaagtctgtc catgagtctt
     4981 tctgatgatt tactgcgtaa caatgcaagc agttgtttga tgtaaagatc ttcaggcatc
     5041 tctgctttta gctgtatgcc tcttttttcc ataatatgat acacttgttg aaagtgtttc
     5101 aattcttcca atgcagtttc aatcagttca ggaataattt ttgttttgtc ggggcatttt
     5161 gacacaaagc tcattgccat agcagaggct tttctttcgc aatcggcatg atcctgaaga
     5221 aaagaatcaa aatcattcat cactgcatca acccaagctt gcgtagtgtt ataagttaaa
     5281 tcaatagaaa gtttcatttc gtttttaaat atttgtacaa aagtaatttt ccgttgaata
     5341 agccacatac ctatttcgaa aagcaacagt ttgttccttt actgcaggag cctccacatt
     5401 cacagctcca atgcattgta gttattcctt gttttaatga acctgattta attacaacgc
     5461 ttgattcgct gtggaaatgt gatgaaacaa aatttcctgt tgaaattatt gctgttatca
     5521 actcaggaag aaatgctgat tccaatgtta tggattgtaa taaccttaca ctaaagacaa
     5581 tgcaactttg gatgcaaaaa cacaattcaa atcacaggca ctatcaagtt attcattgta
     5641 gcgacttgcc tcctaaaaaa gcgggtgtcg ggcttgcacg taaaattggt atggacgaag
     5701 cagcataccg ttttagttta aatggtaatg ataatggaat aattgtttgt ttggatgccg
     5761 attgtacagt gcagccaaat tatttggttg agattgaaaa acatttcagc aacaatataa
     5821 aaactgcagg ctgttctgtt tattttgaac atcctctgga aggaaaacag gagccttcaa
     5881 tttatgatgc tatcatcaat tacgaattgt atctgagata ttatgttggt gcattgcgct
     5941 attgtggttt tcacgctcca tatcacacca ttggctcaag tatggctgta acaaataaag
     6001 cctatcagaa acagggagga atgaatcagc gaaaagccgg agaagatttt tattttctgc
     6061 ataaaatatt tcatctcgga aatttttctg agcttaatac aacaaccgtt tatccttcac
     6121 cgcgcatcag taacagagtt ccatttggta caggcagtgc catttcaaaa atagtaacag
     6181 ccggtaataa taattatcct gtttatgcat ttgaaacttt tgaaattctt aaagtatttt
     6241 ttgatcagat aaaagtaaat gcaacatcaa gagaaatgat aaatattgat tcatttgctc
     6301 ctgctttgaa gaatttctta tcggcacaca atgcagcatc aaagatgata gaaatagcaa
     6361 cacagacaat caacagcaaa acctatctgc aacggtttgc tcattggttt gacggtttta
     6421 tggttttaaa atgcgttcat tatatgcgtg atcattttct ggagcaagag gaagttttgg
     6481 ttgccacaaa aaaattgatg aacaatttga atataagtcc cggtaatttg actaaacgtg
     6541 aaatgttgtt aaggttgcgt aagcacgacc gttcaggtag ttgagaagtg cctatcttcg
     6601 tgtcatgaaa ttgttgaatt acattaacgg gcagatggta gaacccatca gtcagaaatg
     6661 gatggataat acagacccat ctaccaacac agttttttca caaatacccg acagcgatga
     6721 acgtgatgtt cagatggcag taaatgcagc aaagcaggcc tttgtgggat ggagtactat
     6781 gcctgtaaaa aaacgctctg atttattgct gagaatagca gcactgattg aaaaaaacct
     6841 gcaacgattt gccgaagccg aaagcctcga taatggcaaa cctgtttggc ttgccaaaca
     6901 agtggacatt ccacgtgcat ctgcaaattt tcatttttat gctactgctg cattgcatct
     6961 tgctgccgaa acacatgcca tggaaaacac ggcaattaat tatactttac gtcagccaat
     7021 aggtattgca ggttgtattt caccctggaa cttgcctttg tatttattta cctggaaaat
     7081 tgcacctgca ttagcatctg gaaactgtgt ggtggcaaag cccagcgaaa ttactccggt
     7141 aacagcattt ttactaagtc agttttgcat tgaagccgga cttccagccg gtgttttaaa
     7201 tattattcac ggtacaggtc cacgtgtggg cagcgctata gtttctcatc ctgatattcc
     7261 tgtaattagt tttacaggcg gcaccaagac tggtgcagaa atttcaagaa ttgccgcgcc
     7321 aatgtttaaa aaactttctc ttgaattagg aggcaaaaat cccaatatca tttttgaaga
     7381 ttgcgatttt gatcatgctg tttctacttc aataaaatct tctttcagca atcagggcga
     7441 aatttgcctt tgcggctcac gcatatttat tcaacgcagc atttatgata aattcaaaga
     7501 agcatttatc agcaaagtta aaggactgaa aacaggcgac ccaaaagatg agtcaaattt
     7561 tctgggagct atagtatctc aaccacattt tgaaaaaata ctttcatata ttgatattgc
     7621 caagcaggaa gggggtgtaa ttttaaccgg aggcaaggcc atacaacctg aaggacgctg
     7681 taaagatggt ttctttattg agccaaccgt aatcgaaaat ctgccttata actgtcgcac
     7741 caatcaggaa gagatttttg gacccgttgt gacactaact cctttcgaca cagaagaaga
     7801 agttttaatg atggctaata gcacaaatta tggattggct gcaacagttt ggacaaccaa
     7861 tctgacaaag gcacataaag tggcagcagc ccttcatagt ggtattgtat ggataaactg
     7921 ctggctttta cgtgatttgc ggacaccttt tggaggtgta aaacaaagcg gtattggccg
     7981 tgaaggtggt tttgaagcat taaagttctt taccgaagaa aaaaacatct gcattaaact
     8041 ttaacctatt acttttgccg ccactatgtt tataactgta ttaaaatcga aaattcatcg
     8101 ggctaaggtc acacaggccg aattacatta tgtgggaagt attacccttg atgaaaacct
     8161 gatggatgcc gctgacctga ttgaaaacga acaggtgcag gtgcttaata ttaataatgg
     8221 tgaaagattt gttacctatg ttatcaaagg agaacgcaac agtggtgttg tttgcctcaa
     8281 tggtcctgca gcacgcaggg tgcagttagg cgacatcgtt attgtagctt cttatgccat
     8341 gatggaaaaa gaagaagcca aaaattttaa acctgtcttg gtttatcctg atgaaaaaaa
     8401 tcaacctgta aaataaatcc cgacataggt tgaagaaaaa agttgtttca gtaataaagt
     8461 atgcattgtt tctggctgcc ggtgttggta tgctatggct tactttccgt aatcaggatt
     8521 ttacacaggt aattcagaaa attgaaaatg tacatatcgg ttggctgctg gcatctgttg
     8581 ctgcagcaat acttgcacat attagtcgtg ccatcagatg gaatatgctc attacacctt
     8641 taggttataa gccaaaactg attaatactt tctgggcatt atgtgcagga tattttgcca
     8701 atctggctgt accacgtctg ggcgaaatca ctcgctgtgg catgctcacc aaagccgagc
     8761 aagtaccttt taacgaactg ttcggaacgg tgattgtaga acgtatcatg gatgtactga
     8821 tgttgttttt gttattgctt gctgttgcaa cattagagtt tcatctcatt ggtggttttt
     8881 tatatgataa tattgctgca cctttgatga gtaaatttca aaacctaaat cttccattag
     8941 taataggtgt tttagttgtt ggcatcattg cagtttttgc ttatttctat ttctcaaaac
     9001 attcaggaaa gagcaaagcc attttcgaaa aaataaattt attgcttaaa ggtgttgctg
     9061 atggcattaa gtcaatagca aaaatgaaaa acaaatcatt gtttattttc cacaccctat
     9121 ttatttgggt gatgtatttt tttgcttcct atctgtgttt ctttgcattg gattccacat
     9181 ctatgctcga tgcaaaagca ggtttgttta ttcttgtcat aggtggattg ggaatgtctg
     9241 cacctgtaca gggcggaatt ggggcttttc acttcatcgt ttcacgcgga ctgatgcttt
     9301 atggtatttc tgaaacagaa ggaattgttt atgcaaccat tgttcatggc tatcaaacca
     9361 ttttagtgat agctttgggt gctataggat tctttgtatt gatagcaaca ggaaaaagaa
     9421 ttaaagccaa caatggatta taacgagata atcagcaaga aaatttttaa tgagcaatcg
     9481 ttattgcaac aacttagcat ttggcgcttg caaaatcaaa gtcttgtttt taccaatggt
     9541 tgttttgaca ttcttcactt ggggcataca caatatttat ctcaggcaaa aaactatggc
     9601 gataaattaa ttcttggtct caattccgat aaatcaggtg cttgtaaaaa aggtctgggc
     9661 agaccgctga ataatgaaaa tgcacgagcc tttgttcttg catcgctaca tgttgttgat
     9721 gcagttgtat tatttgatga ggaaacaccc tataatctga taaagctcgt tcaacctgat
     9781 gtgctggtaa aaggtgccga ctatgaaatt gaaaaaattg ttggctacga tatcgttata
     9841 caacgtggag gcaaggtcat taccatagat ttaacacagg gatattccac tacagccctg
     9901 ctaaatcgca tcaaacaata aaaattttta aataaagttt ctgaatttaa aagctttgtt
     9961 gtaaatttac ctttcaatta tagaacttat gaagcaggct gctataaaaa ttccaattgt
    10021 tgccttatca ctactactat taatgggttg tgaaagcaat cacttcttta aatcagaaaa
    10081 aaaattaaag agtgatattg ttggtacatg gaacagagta ttgtttgggc aacagaatga
    10141 agaaacatgg atttttgttg atggtgcaat aaaaatttat cagcctaacc gtcatggcga
    10201 tactgctgat gtgggtacat tttctattga tgctacatta gcatcgccct atttaaagat
    10261 tgaaaataca agctatttaa atcagaatcc agggggcgac acttataatc acaagtggtc
    10321 aatagtaaaa cttgataata aagttctgga aattgctaca gacagcccgc tgggaggagt
    10381 agcacagatt gaatttatca aacaatgata tcttagcgtc atcatttttt ttcagacgct
    10441 gtaatggcta aagtaaaatc agtttttgtt tgtcagaatt gtggcgcaca gtcacccaag
    10501 tggatgggca aatgtgcatc atgcggtgag tggaattctt atgttgaaga acttatcacc
    10561 tccggaaatt cttcggcaca ggtaaattat aaacgcagca gcaagcctga aaaaattaca
    10621 gaaatagagt tgcatcagga gcagcgaatt gtacttcccg accatgaact gaatcgtgtt
    10681 ctcggtggcg gattggtacg tggctcactc attctcttag gtggcgaacc cggtatagga
    10741 aaatctactc ttttacttca gctcgctgta agattaaaaa atctgaaaac agtttatgtt
    10801 tccggtgaag aaagcggtca gcaaattcgt atgcgtgcag aacgacttaa ttcagacctg
    10861 cacagccaat gttatctact tacagaaaca tcacttgaaa taattttaca acacctcaat
    10921 gcattgaaac ccgacatggt tattattgat tctatccaaa ccatgtttca ttcgcagatt
    10981 gaatcatcac cgggaagcgt atcgcaaata cgaggatgtg caacagagtt gctgcgtttt
    11041 gcaaaagaca gtggttgtcc tgtattttta gtcgggcata ttactaaaga aggtgtcatt
    11101 gcaggaccaa aagttttaga acatattgtt gatactgtgc ttcagtttga aggtgacagg
    11161 catcatattt accgtttgtt gcgcagtcat aaaaatcgtt ttggctctac agcagaaatt
    11221 ggcatttacg aaatgcaggg taatggtctg gcagaagtat cggacccatc gcagataatg
    11281 ctttcgcaac gtgatgaagc actcagtggc attgccattg cctgtaatct tgaaggcatg
    11341 cgcactatgt tgattgaaac gcaggcattg gtaagcagtg ctgtttatgg cgtgccacaa
    11401 cgttcaacaa cagggtttga tcaaaggcga cttaatatgt tgcttgcagt acttgaaaaa
    11461 agatgtgggt ttaagttggg tgctaaagat gtttttctca atattaccgg agggttaaag
    11521 gttgatgatc ctgcaattga tttagctgta atttgtgcta tactttcaag taataatgat
    11581 attcctcttg agccgctgac atgctttacg ggtgaagttg gattatcagg cgaaacacgt
    11641 ccggtaagcc gtattgaaca aagaataaaa gaagccgaaa aacttggttt caaaaaaatc
    11701 tatttccctg cacagaatct ggctgccttc gataaagcca tgtttaagaa cattaaactt
    11761 tatccggttg aaagggtaga tgaagttttt tcattgcttt ttggataatt ttttctgaaa
    11821 agacttcttt attcaaaaaa tccattatat tttagtcggt aaatatttct ttatttaaat
    11881 agaaaaacaa tgaaaacctt aatttcagca gtaatagcta tggtttcatt taccattagc
    11941 gcatcagcac aaagcaacat tgaaattggc atgtcgggtg gtgccacaaa ttattttggt
    12001 gatttgggta ataatgaagt actgcaatct acttcaacac gacccggtgg cgcaattact
    12061 atccgcaact ttttaaatag ccctgctaag acaggttatc agtatcagcc attatctgtt
    12121 gaagcacgtt ttagctggca tcgcatcggc tatgatgaaa caaaatctat tggcgataaa
    12181 aaaggatacc aactgcgtaa ttacggaagg ggtttaagtt tcaggacaga tgtttatggt
    12241 gtgtcaacac atgttagcta tactttatat gccaataaaa gaaagccatt gtatgcacaa
    12301 ggagcagcaa tgtactttta tgcaggtgtg ggcatttatt atgcacggcc taaagcagat
    12361 ttatttaatg gaagcattga catcaacaac cgttattatt tctggagcga tggcaccatc
    12421 agagatcagg atgaaaaaac tggacaaggc aatataataa agcgtgatgg taagtacgaa
    12481 accaaccttg atgaatggcg cacagaagga cagggatata acagtgagtt ggggcagaaa
    12541 aaaccataca accttatgca cataggtttt ccattgggaa tgggatggcg ctggggtatt
    12601 tctaaaaaca ttattctctc ggcagagctg agctatataa aatttattac agattattta
    12661 gatgatgtaa gcgaccgcta tgcaacctac gaagaaataa aagcaaattt ccccgaagaa
    12721 cataagcagg acttggccaa gtatatctct gacccttccg gtaagggcac aaatggtttt
    12781 gaaggtgttg caacatcaca acgtggcaac catagaaaaa cagatggcta tgcatttctt
    12841 tcacttgaag ttgcctacag acttgatttt aactggggaa aaataaaatc atctttttaa
    12901 gaaaattgct ttttactttt tgtatcgttt aatcattgtt gcatttttgc agcaatgcaa
    12961 ttaacaacca ttccttttca acttcgtttt aggaagccat ttgttttagc ttcgggaaca
    13021 agaacacata catcagtagt ttatattaaa gctatacaaa atgaaattac cggctggggc
    13081 gaagctgcaa tgcctcccta tgtaaaagaa acacaagaaa gtgtaataca ttttgtacag
    13141 caactgcata ttcctataat aaacacgtta gatgatttaa acctattttt tgaaaaacta
    13201 tatcaaacat caggaaatta ttttgccaaa gcagccatca atggagccat gctcgattgg
    13261 tggacaagaa aaaatagtct atcactgtcg gaatattttg gtattgataa gaacctttca
    13321 acacctgatt gttgttatac cattacaaat ggtgatgata taaaacattc aattaagcaa
    13381 gcagtagatt ttaaattact gaaaataaaa gcaggctttg atggtgatgt tgagtttata
    13441 aaagaaattg ctgcaattag tgaaaagcag atttgtatag atgctaatca aggttgggtt
    13501 aatgtgaatg ttgcattaca aaaaacagcg cagctgaaaa atttgaatat tgcttttatt
    13561 gaacaaccac tacctgttga cgattggcaa ggtatgcaga aactaaaaca acataccaca
    13621 tttaatttta tagcagatga gtcatttcag actattgatg atttaaaaaa aataatggat
    13681 tgtttccatg gagtaaatat caaactgatg aaatgcggtg gcgtatcaaa cgcttttgaa
    13741 attgcaaaat cagcacgaca gttaaatgcc aaactgttat tgggttgtat gagtgaaagc
    13801 agttgtggta tagcaacagc tggagccata caacaattat ttgactgggt tgatcttgac
    13861 ggacccttat taatcagcaa taatcctttt accggagtca gatatcaaga agggaaaata
    13921 tttattgaaa ataatgtagg cacaggggct ttgtctgttg aatctacttt gcaggctctt
    13981 taaatacaag ctccatcagg tgtacgtcaa catagtttcc gaatttaaag cctacttctt
    14041 tcattacacc aatgtgtcga aaaccaaaaa tggtatgcaa atgaatgctt gcctcattac
    14101 ctgatgcaat tcttgaaagt agatagtgca accctttgct ttcaccttta agaattaata
    14161 tctctaaaag agctttacct attcctttgc cacggtgatc tttatgaaca taaacagaaa
    14221 cttcagcagt accattgtaa gcacaacggt cgctccaggc ggttagggat gcccatccgc
    14281 aaagcacacc attatcttca gcaacaatta tgggatggct gctgtcatga tttttaaacc
    14341 aaagcatcct gtcttcaata ctttttactt ctgtatcgaa ggtagcagtt gtattcaaaa
    14401 tagcatcatt ataaacttcg gtaataaacg gaatatcgtc tgcagtggct gatctgattt
    14461 ctatcataag ttgtggtata gaaatacaaa agtataaaca atacgatatt tttctgttga
    14521 aagtaattaa tggattacaa aaactaagat agagctaaaa ttcttacaag cggtttttta
    14581 aatgaataca gtgtttgttt ttgttttttc agagatataa cgcaaattaa tatttgaaaa
    14641 tttcaacgat tcttttaaaa aaacttacag tagtaagagt tggaatttgg tgacaaagaa
    14701 aacacacaat gtggtaatgg atttgcaatt acccctacaa attcaaaaca aaaaaaatat
    14761 ggacttatta cttgcaatct taatttcaat gggaatcaac gtaaccccaa aagatttaac
    14821 taacaaagag tttatgctca aaaatcaaag tgcaatacaa catgcacagg caattttaaa
    14881 agagcgtcag acagagtcaa cacgcataac ttttaaggct gcgccacaga caaatatttt
    14941 caagcaatct aaaaattaat ttccgtaaca tctttcactg atataaagcc gcctcagttt
    15001 ctgaggcggc ttttttattt atagtaatac tttatcaata tccggaaaac ggaaatgtaa
    15061 caagaaagta tcgtttaata ttttaaatta ctgaatcaaa tactttctta caataaaaat
    15121 agcagtattt tcaattactt ttaagttatt tacctctgaa agagtatgta gtcagaaatg
    15181 atgaaagctg aaatcttaca aaagaaagtt acttacttaa aggagatttt taaaaatgaa
    15241 ttcggcaatg catttgcaat tactcctaca aattcaaaac aaaaaaaata tggacttatt
    15301 acttgcaatc ttaatttcaa tgggaatcaa cgtaacccca aaagatttaa ctaacaaaga
    15361 gtttatgctc aaaaatcaaa gtgcaataca acatgcacag gcaattttaa aagagcgtca
    15421 gacagagtca acacgcataa cttttaaggc tgcgccacaa acaaatattt tcaaacaatc
    15481 taaaaattaa tttccgaaac atctttcact gatatatagc cgcctcagtt tctgaggcgg
    15541 cttttttgtt taaaatatta gtgtgcatgt tgaaaattat ccaaacaatt tataaccgaa
    15601 atccacgatg caaatttgat aagacccgaa atggatttaa attttatttc aacagcactt
    15661 cagaatcctt ctctacatca aaagtatagc atatttctgg aaagagtaca attgagctat
    15721 ctgattttat atccaatcag aatccccact gatttagaat atggaagagc cttttattta
    15781 agttaacaca actgctacgt ttttctaccc cattttaata taaaacaaca ataaatttta
    15841 aagttccctt cattattatt ttttcatctg ttaagtaaca tctcttagta tttcttacat
    15901 aagatttttc aggtttaaca gcaacttaaa gaaaatttct cctctgaaaa caggaaataa
    15961 actcttacag ttagttcatc attcttacaa aagaaagaaa gcccatttcg aggtcagaaa
    16021 aaactgagta gcggcaattg gtttgcaatt acccttacaa attcaaataa aaaacaatat
    16081 ggacttatta atctcaattt tactgtactt aggtgtttat gctacaccgg ctgatcttca
    16141 aaacaaagag tttgttgcta aaaattcaga acaaatcaac aaagcagaaa tgctttcaaa
    16201 agatccaaac tttaaatgtg atggaattgt aattgtcggc agcggtaatt tgtaattaac
    16261 gtctccaaaa gaatatcaaa ggcgctgact ttaaatggtc agtgcctttt taatttttct
    16321 gaaatggctg cccagagttt ttcataagga attacttcaa gcccttcagt attaaaaaat
    16381 ttattgccgg caacaggttt taatttttct aacgatacag cagtttgttt ctgtgtctta
    16441 gttttttgaa agttattttt aaataaggtg agcaacatgt tcagaaaaat atatgtacgt
    16501 ggataaattc tcttatctac atatttttta atatacgatt ccagataact tgttctgtca
    16561 taaagtttgt catagtcgcc catttcaatc atcactatta tctgaccaat catgatgata
    16621 atgttattgt cttctttatg tgtggtgtag tagcttattt catccaaaaa gcccaacaag
    16681 ttgatgcttt cttttggaaa atgaccgggt aagataaaat taaggtaagg ttcaaaataa
    16741 tgccaacgct cttttacttc ctgattggag ttgataaagt attcactgcg tgttgcttcg
    16801 taataaatgt ttaatgcctg ttcaaaattg cctgagtgca tacaaagaat aaagtaatgc
    16861 tcgagcgcat gcagccagtc agacgagttg gcactgagtt tacctaaaaa aatccgtgct
    16921 tcttctttac ctttttcgta ctgcctgagg tggatgtagg ccaacattct tgaaagcgca
    16981 ataaaacttt tctgaaagtc ggaatagtat tcgggatgat tctgataaaa cgcttcgcgt
    17041 tcattcaagg cttcttctac caattcaaaa ttactgctga aatagccata aataaaagca
    17101 atgttgccgt aataaaaatt tgaattgaat gttttaagtt gctgatgtat ttttttagct
    17161 ctcagataat atcgtttagc cttggcagca tttttatact gtgaaatatt tgaagctacc
    17221 tgaaaaatat ccagttctct ctgcatcaat atcaactcgg cttctaattc aaactgtttg
    17281 atagattttt taaagcggtt tctgtaatga taaaaatcgt caggtttatt tttaatagcc
    17341 atcctgtcgg caagcatcag gtcggtaagg caaccaatca tgccatcatt tgtaaatgtt
    17401 gcatagtctg ctgctttttt aaagaaatgg tcagaaaaaa aagcggaggc attctgctga
    17461 cgtaatatca tggcagcacc aaaataaatg tatgcacgaa aagaacttcg ccccaacatg
    17521 gagttcatca gtttttcggg atctaaccca aaaaccagat ttaccaaacg atcttcaagt
    17581 tcagctttca gttgctgata ggcaggattt gtggctgtgc tttgtaatag tttttcggca
    17641 gcttcctgat cgttgttgat gctgcctttc gaaatctctt tataaaacca aagcagctca
    17701 ggataatttt tatcagcaaa ataaacatca ggcacaccaa gaaaatcatg acggtgttta
    17761 gcaaaaaaat taattaataa tttgatttca gaaaactcca tcttataccc gataaatcaa
    17821 tgctttattt tctttcaaat ataggtcatc aataaatgat gtactgcaac gttgaaattt
    17881 tatttttcag atggcttgtt aacagcctgc attatatttc aagcttggtg caggctcttt
    17941 ctgctgcaat ttgctctgct tttttcttta caaaatccat ccctgtggaa acttcttctc
    18001 cgttaatcat caaccgcact cttatgagtt tgtcattgct tttttcgtct tcaacagttt
    18061 caaaggcaac agtgtttttt gttcgctgcg accagttgag tatgcggctt ttaaaattta
    18121 tttcagttgc ttccagcgat tccagatcaa catggtggtt aatcattctt tcgagaataa
    18181 actgacgggc tttgccgtag cctctgtcta tataaatagc accaatcaat gcttctaatg
    18241 catcgccata aatgttgcga aacgaatcgg gccgtggggg ttttttttta attttttttt
    18301 taaaaagttg tagggcacag ggtgtttatt ttaggggggt tgcataattc gggggcttt
//
LOCUS       DAOOOI010000044        17290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_666_length_17290_cov_26.576733, whole genome shotgun sequence.
ACCESSION   DAOOOI010000044 DAOOOI010000000
VERSION     DAOOOI010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17290
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_666_length_17290_cov_26.576733"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PKX59_06855"
     CDS             <1..532
                     /locus_tag="PKX59_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOZ90624.1"
                     /translation="FLSANPIETSQLNLEKEKNEIKDVLNSNFTSVFFKFFSFDSVKP
                     SNMVEEMIKLEPTILHFSGHGSESGIILLDEKLHANFIEGKRLADFFSNFKDLQLVVL
                     NACYSQKQAKEIKKYVPYVIGMNAPISDNSAITFSKLFYIALSNGFTIEESFLKAKAG
                     VNFVKTDSGEMITLLK"
     gene            735..1964
                     /gene="serA"
                     /locus_tag="PKX59_06860"
     CDS             735..1964
                     /gene="serA"
                     /locus_tag="PKX59_06860"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOZ90625.1"
                     /translation="MSLTSFPKEKIKILLLEGVHPAAVAMFKDAGYTQIETTAGALSE
                     KELLQKINNIHILGIRSKTQLTAAAIAQADKLIATGCFCIGVNQVSMGDAIENGIAVF
                     NSPFSNTRSVAELVIANCIMLLRRIPEKSKAALAGQWLKDSAGCFEVRGKTLGIIGYG
                     HIGSQVSVLAEALGMQVIFYDVEPKLSLGNAKACKNINDLLRQSDIVTLHVPGTSATR
                     NMVNESFLRKMKKGSVLINLSRGEVMDEKAIAKAISEKHIAGLAADVFGDEPQHKGDI
                     FQSPLQNLNNVLLTPHIGGSTLEAQENIGTDVAGKIIRYLETGNSMGSVSVPALNLPL
                     QQSAHRILHIHRNVPGVLSEINTIMSKHKANILGQYLQTNNTIGYVAVDIDKKASSGL
                     ISDLKNSKETIRVRGVY"
     gene            complement(1972..2313)
                     /locus_tag="PKX59_06865"
     CDS             complement(1972..2313)
                     /locus_tag="PKX59_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOZ90626.1"
                     /translation="MHHFLFSYGTLQLDQVQLENYGRKLKGNKDLLKGYRIEDLEIAD
                     SEVIRKSGKHIHPVAVHSGNENDIIEGMIFEITEDELQATDKYEVSDYMRVIEKFESG
                     KEAWIYIGKKK"
     gene            2596..2949
                     /locus_tag="PKX59_06870"
     CDS             2596..2949
                     /locus_tag="PKX59_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90627.1"
                     /translation="MKRFKILTIILVAAILPALAQNTQPPVVVPALISQGEVIPYQQL
                     PEVIITPNAAEQKAVELPVVTITANKINEQIYPAVKYGNNYIASVTIAPINVVAHQQK
                     RTFASLFSFLRYFSR"
     gene            3023..3901
                     /locus_tag="PKX59_06875"
     CDS             3023..3901
                     /locus_tag="PKX59_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ90628.1"
                     /translation="MKLTIKGLTKTYPNGVQALKNINLEIKNGLFGLLGPNGAGKSSL
                     MRTIATLQNADSGNIFLDDLNVLEDKEEVKKILGYLPQEFGVYPNISAQELLTHLALL
                     KGVHIKKERDEVVKSLLMQTNLYEVRNKNLGGFSGGMKQRFGIAQALIGNPKIIIADE
                     PTAGLDPTERNRFLNLLSEISEQVIVILSTHIVEDVKELCSQMAIINKGEVMFSGHPA
                     EAIKQVQGKIWMCQVDKPTARELMIKYNVISQKFNTGLIELHVWSEEPLNHHFAIAQP
                     VLEDVYFSVLNGNLKP"
     gene            3905..7480
                     /locus_tag="PKX59_06880"
     CDS             3905..7480
                     /locus_tag="PKX59_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOZ90629.1"
                     /translation="MWSVLSFELSYRFRRPATYIYLAILFLISFLFTATDAISISGAV
                     GNIHKNSPYTIYQVMLILTLLGTMIISAVMGVPVYRDYEHRFHEIMFTLPVTKAQYLG
                     GRFLGSYFTALFIFLGIPLGILTGLLMPWIDKEVIGPFMLNAYVLPFLYIIIPNVFIL
                     GVMFFATGSLFRNQLAIYLQGMVFFILYLVLTSATGDVENNPKFALLDVFGITPSFYI
                     TRYWTPFEKNLQTIPLESYLLWNRLLWVGIALIGAFVTYRLFQFSKSSPFVLRSRKNQ
                     EKISVENLSHINLPETSKDYSFSTKLRQWLQMVRFDFFGTIRSVPFIAIWICGVILIL
                     GQMPQIGKMFGTTIYPVTYAIIDDLTGNFTLFTLIIITFYSGELVWKERERGIHLITD
                     SLPVSRYAMITAKFFAMCLIVAMLQLTVMFMGIFIQTLKGFYRYDIDVYLKTLFVSQY
                     PSLILLVLLAFFIHSMVNNKFIGHTLMIAYFVGRMSAAAMGITHKMIWYGNIPSAPYS
                     DMNGYGHFLYPKYMFLIYWGSLGLVLFIIGVMMNRHGSETGIKARWQIFKQQWKFSRG
                     RKIVWSLLLVFIVSGCFVFYNTNILHKFKSDKTVRHEQAEYEKRFRKYLDFNQPRITA
                     VNVNIDLVPEKRYAAIKGYYIIQNKSALPIDTFLITWAHENIKINRFGFDILADEVEN
                     YQKQFVVYKLAHALKPGDSTKVYFDIEYINKGFANSEEELGLVNNGTFINNNYLPAFG
                     YSESFELTDNSDRKKEDLPRKNHTMRLLSDSASRNNTYLANDADWIRYESIISTSPDQ
                     IAVSPGYLQKEWTQNGRRYFHYKMDAPMVNFYSFLSARYEVFRDSFQGINIEIFYHKT
                     HSWNIEKMNKAIKKTLAYCQKTFSPYQFRQVRILEFPRYETFAQSFPNTIPFSEAIGF
                     IYDVSEDTDIDNAFYVTSHEVAHQWWGHQVCGANSQGSTLMVESMAQYTALMVMEKEF
                     GKDAMEKFLKYELDGYLSGRSSERINEQPIARNDGQGYIHYQKGSLVMYAMKDYLGED
                     TLNAALHRFIKTYGFQSPPYVQAPQFVQYIADATPDSLKQVVEDMFNRITLFDLKCVE
                     AGYKKKGNKYEVTLDVETGKVYADSMGNGTGVKPNDWIDIGIMKYDGNGKHSYQYLQK
                     QLFTGGRKTIVVTVDEKPSKAGIDPLHKLIDRNSEDNIKEVKSLQ"
     gene            7520..9109
                     /locus_tag="PKX59_06885"
     CDS             7520..9109
                     /locus_tag="PKX59_06885"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ90630.1"
                     /translation="MLEVKNLKVVFHQNSNPVVAVNDFSFSIAAGETLGLVGESGSGK
                     TVSSLALLGLLNNKNVAISGNASFSLQQPVDLLTLNEKQLTAIRGRHIAMIMQEPMQA
                     FNPLFTCGHQVTEAIRKHTALNKNDATEKVLQQFEKVKLPNPLQISKRFPHQLSGGQL
                     QRVAIAMAISCNPSLLIADEPTTALDATVQKDILLLLQQLQSENNMSMLFISHDLEAV
                     KLIAHNTLVVHAGQMVEQEKTEKIFAHPAQEYTKELLQSRPSGFKRNSVISEKNILSV
                     ENISKSYNQRQGLLRSKQNIIKAVDNISFTVKQDETLGIAGESGSGKTTLAKLLLRLI
                     ETDSGVIKLSEENLKDYPIRTKQLWRKKIQAVFQNPHGSLDPEMTVGRQLTEVFKLHF
                     SSQNTTTVKNQIIEWFEKVGLTEQHFNRYPHELSSGQKQRLCIARALIPQPDLLICDE
                     AVSALDVSVQAKILELINRLQQQFHFGCIFISHDLKVIRQMCDNVLVMKDGKADCYSA
                     TEKLFEKPPTTYSEILLKSII"
     gene            9113..9559
                     /locus_tag="PKX59_06890"
     CDS             9113..9559
                     /locus_tag="PKX59_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ90631.1"
                     /translation="MINIRKGTPADVEPALRLIKELAEYEKAPEQVINTVEQMLKDGF
                     GEKPIFDLLVAETNDNIVGIAIYFIKYSTWKGKCLYLDDIVVQESLRGKGIGRKLFDA
                     VAAEAKLQNCQQLQWQVLNWNEPAINFYKKYDTVFDDEWINCKIIL"
     gene            9714..10409
                     /locus_tag="PKX59_06895"
     CDS             9714..10409
                     /locus_tag="PKX59_06895"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOZ90632.1"
                     /translation="MHPEVRKIFKSITVPLLLSLLMIGVKIFEVLSHTDLGEYGVLPR
                     QISGLKGILFIPFLHADWEHLFSNVVPLLVTGSMLFYFYKEMAVKVILLIYILSGFWL
                     WLGGRENYHIGASNIVYGITAFLFLSGFIRKHTGLIAVSLLMVFLYGSLIWGLFPVVP
                     KISWEGHLFGALSGILCAIVFRKEGQQKEVYEWNDDEPFDDQENDDNATQSSLKIVYS
                     YKKSDEKNGTEES"
     gene            10396..10800
                     /gene="mce"
                     /locus_tag="PKX59_06900"
     CDS             10396..10800
                     /gene="mce"
                     /locus_tag="PKX59_06900"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOZ90633.1"
                     /translation="MKNLEHIGIAVKSLQISDALFEALIGCPPYKKESVESENVITSF
                     FQTGETKVELLEATSPESAIAKFIDKRGEGIHHIAFAVDDIKAEMQRLQQSGFTLLNS
                     EPKKGADNKLVCFLHPKSTNGVLVELCQEIKS"
     gene            complement(10797..11423)
                     /locus_tag="PKX59_06905"
     CDS             complement(10797..11423)
                     /locus_tag="PKX59_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOZ90634.1"
                     /translation="MIKNIIFDFGGVLLPINTEKTVLEFGKMGLDNFDELYSMQKQAK
                     FFDDFEKGLIPASAFRSEIRKHYKRPLSDEEIDYAWNALLGELNPERFNFLSELRDKY
                     NIYLLSNTNIIHHAAFIKSLNQTFGKDRFEKLFEKTYYSFQMGTRKPDKRIFADVIKD
                     SGVTPAETVFIDDNLNNALGAQKVGMNAIHHDNAMEIEKELMAMLSAF"
     gene            11504..11577
                     /locus_tag="PKX59_06910"
     tRNA            11504..11577
                     /locus_tag="PKX59_06910"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11538..11540,aa:Ile,seq:cat)
     gene            11677..13614
                     /locus_tag="PKX59_06915"
     CDS             11677..13614
                     /locus_tag="PKX59_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOZ90635.1"
                     /translation="MVARHDPLNKITLGGLLVTLGIIYGDIGTSPLYVMNAIIGDRPI
                     SPELVLGGISCIFWTLTLQTTFKYVFLTLRADNKGEGGIFSLYALVRKTKVKWLAIAA
                     ILGGSALLADGLITPAISVSSAVEGIQILHPDLPTIPIIITIIFGLFFIQQFGSNFVG
                     KSFGPMMLIWFSMLFVLGFHQLIQNPSVLKAFNPYYAYQLLVNYPGGFWVLGAVFLCT
                     TGAEALYSDLGHVGRKNIYITWTMVKFCLLINYFGQGAHLLKFEGKTIDVNPFYQLMP
                     DWFLLIGIIISTLAAIVASQALISGAFTVVNEAMRLNFGPKLKVVYPTDLRGQVYIPT
                     VNWTLCAGCIGVILFFQHSSNMEGAYGLAIIIDMMITTILLSFYLYLKRYPLPLVMLT
                     FLLFIVIEIAFLVANSIKFEHGGYVTMLLTMFLFITMYVWYAARKIRNQFVDFVKVKD
                     FLEILTNLSKDESIPKYATNLVYLTSANRRDEIESKVMYSLLQKFPKRADIYWFVHVD
                     VLDEPYTMEYKVAEMVNDKVIHIDFRLGFRIEPRINQMFRQVVEELVKNNEVDITSRY
                     TSLKEKNVIGDFRFVVIEKTLSYDNKLNFYEKFVLNAYDFLKLFSLPESKAFGLDTSS
                     VTVENVPLVVKPSGKFKLKRI"
     gene            complement(13611..14816)
                     /gene="hflX"
                     /locus_tag="PKX59_06920"
     CDS             complement(13611..14816)
                     /gene="hflX"
                     /locus_tag="PKX59_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOZ90636.1"
                     /translation="MKEKTVFKPLTDVKERAVLVGVILKDQPVEQEMEYLDELAFLAE
                     TAGAQTLKSFTQKLDHTDPRTFVGSGKLAEINEFIKANEINLAVFDDELSPSQQRNIE
                     KILQIKVLDRTQLILDIFARRARTAQAQVQVELAQYQYLMPRLTRMWTHLEKQQGGIG
                     NRGPGEKEIETDRRIVRDRLSKLRERLKEIEKQGFNQRKARGNMVRVALVGYTNVGKS
                     TIMNLLSKSDVFAENKLFATLDTTVRKVVLENIPFLLSDTVGFIRKLPHHLVESFKST
                     LDEVREADILIHVVDISHPNFEAQINVVNQMLQELQSADKPVILVFNKIDAFTYIEKE
                     ADDLTPMTSENISLDDLRKTWMNKGNVETIFISATEKDNISELRTMLLEKVKRIHLVR
                     YPNNKITND"
     gene            complement(14839..15459)
                     /locus_tag="PKX59_06925"
     CDS             complement(14839..15459)
                     /locus_tag="PKX59_06925"
                     /inference="COORDINATES: protein motif:HMM:NF016161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HOZ90637.1"
                     /translation="MYTGILHTHTLVVSLFIVLYLIKATLLVLNRHAALDNFVKKTKV
                     AEMIISTLFLLTGIYLAVNTGNKGTWLWVKLFVIACTIPLAVIAFKKKNSSLALISLM
                     ALIYAYGISETKSISMKKADTAVNTEGLEGAAAGKVIFESKCQSCHGADGKLGLSGAK
                     DLTQSALSPQEKIDIITNGKKTMAAYKDQLSQEQIAAVAEYVQTLK"
     gene            complement(15484..15571)
                     /locus_tag="PKX59_06930"
     tRNA            complement(15484..15571)
                     /locus_tag="PKX59_06930"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15535..15537),aa:Ser,seq:gga)
     gene            complement(15763..16785)
                     /locus_tag="PKX59_06935"
     CDS             complement(15763..16785)
                     /locus_tag="PKX59_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOZ90638.1"
                     /translation="MKTDKKNLLVIYTGGTIGMIHDEETGSLKPFNFGKLSTVVPELK
                     KLPCKIDFHSFDPLIDSSDMQPERWIQIAEVIEENYGKYDGFIVLHGTDTMAYTASAL
                     SYMFQNLNKPVILTGSQLPIGEIRTDAKSNLITAAEIACGNVVVPEVCICFNNQLFRG
                     NRAEKFTSSKFDAFQSINYPSLAEAGTEINYNHDAIREVNNNALKVKTHFNNSVMIFK
                     LFPGVNEKVLSAILKSGALKGLIIETYGSGNAPKNEKLLKQLGDAVKNQMVIVNVSQC
                     SGGVVQMEKYENGLALKRAGVVSGHDMTTEAAITKLMFLLGSEMDYQEIKVNMQLDMA
                     GELSRE"
     gene            complement(16785..>17290)
                     /locus_tag="PKX59_06940"
     CDS             complement(16785..>17290)
                     /locus_tag="PKX59_06940"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOZ90639.1"
                     /translation="KYFGVGEIGLDFYWDKSFIKEQEEAFLKQITWSCELNLPVSIHS
                     RSATTRAIELIQLSGLSVKGVFHCFSGSIEEAKAITDMGMYLGIGGVVTYKNSGLDAI
                     VSQVGLKHIVLETDAPYLPPVPHRGKRNIPEYLLLTAQKLAVIKNCSVAEVAEQTTNN
                     SKVIFKQ"
BASE COUNT         5427 a         3190 c         3163 g         5510 t
ORIGIN      
        1 gtttttatct gcaaatccaa ttgaaacctc tcaactaaat ttagaaaaag agaaaaatga
       61 aatcaaagat gtcctaaact caaattttac tagcgttttt ttcaaatttt tttcttttga
      121 ttcagtaaaa ccaagtaata tggtagagga aatgataaaa ttagaaccaa caatattgca
      181 tttttctgga catggaagtg aatctggtat tattctttta gatgagaaat tgcatgccaa
      241 ttttattgaa ggcaaaagat tagcagattt tttttctaat tttaaagatt tacaattagt
      301 agtgttaaat gcatgctatt ctcaaaaaca agcgaaagaa ataaaaaaat atgtacccta
      361 tgtaataggt atgaatgccc caatttcaga caattcagcc atcacttttt caaagctatt
      421 ttatattgcg ctttcaaatg gttttacaat agaagaatcg tttttaaaag caaaagctgg
      481 cgtaaacttt gtaaaaacgg attcaggaga aatgattaca ttgcttaaat aatcactaat
      541 ttcttgcatc cattcaaagt cccctgaaat acgggagact tcgaatggac aaaatatact
      601 tttttaaaaa tcgttttaat attttcgcta taaattaatt ggttttatct ttcctaaact
      661 gaatgtaaat aacttgtctt atttacacta ttccggtcag ataaaacaaa ctatcattgc
      721 atcaaaattg atttatgtcg ttgacaagtt ttccgaaaga aaaaattaaa atcctattgc
      781 ttgaaggtgt tcatcctgct gctgtggcaa tgtttaaaga tgctggatac acacagatag
      841 aaaccactgc cggtgcatta tcggaaaaag aactgttaca aaaaataaac aatatacata
      901 ttcttggcat acgttcaaaa acacagctta ctgctgctgc tattgcacaa gcagataaac
      961 tcattgcaac aggctgtttt tgtattggtg tcaatcaggt aagtatgggt gatgccatag
     1021 aaaatggaat agctgttttt aattcgcctt tcagcaatac acgctctgta gcagagttag
     1081 tgattgccaa ttgcattatg ttgttgcgca gaattccgga aaaaagtaag gccgcccttg
     1141 ccggacaatg gttaaaagac agtgcaggat gttttgaagt tcgcggtaaa acattaggga
     1201 taataggata tggccacata ggcagtcagg tttctgtatt agcagaagca ttaggcatgc
     1261 aagtaatttt ttatgatgta gagccaaaac tgagtttggg aaatgccaaa gcatgtaaaa
     1321 atataaatga tttactcagg caatcagaca ttgttacact gcatgtgccg ggaacttcgg
     1381 caacgcgcaa tatggtgaat gaatctttct tacgaaaaat gaaaaaaggc agtgtgctta
     1441 tcaacctgag tcgtggtgag gtgatggatg aaaaagctat tgcaaaagcc atttcagaaa
     1501 aacatattgc agggcttgca gccgatgtat ttggtgatga gccacaacat aaaggcgata
     1561 tttttcaatc gcctttacaa aacttgaata atgtgttgtt gactccacac attggagggt
     1621 caacacttga agcacaggaa aacattggca ctgatgtagc cggaaaaatt atccgctatc
     1681 ttgaaacagg caacagcatg ggcagtgttt ctgtaccagc attgaattta ccattgcagc
     1741 aaagtgcaca tcgcatttta catattcacc gcaacgtgcc cggtgttctt tccgaaataa
     1801 ataccataat gagtaaacat aaagcaaata ttctcggaca atacctgcaa acaaataata
     1861 caataggtta tgtagctgtt gacatagata aaaaagcttc ttcgggtttg atatcagatt
     1921 taaaaaacag taaggagact atcagggttc gtggggttta ttaatgcatt attatttttt
     1981 ctttccgata taaatccatg cttcttttcc ggattcaaat ttctcgatca cacgcatata
     2041 gtcagacact tcatatttat cggtagcctg taattcatct tctgtaattt caaaaatcat
     2101 tccttcaata atatcgtttt catttccgga atggacagcc acaggatgaa tatgctttcc
     2161 acttttacga attacttcac tgtctgcaat ttctaaatct tcaatcctat agccttttaa
     2221 taaatctttg ttgcctttta gttttcgacc ataattttcc agttgaactt gatcaagttg
     2281 caatgtgcca taagaaaata aaaagtggtg catggattaa atttgttgag gcaatattag
     2341 tattaagcca ataaagaatc aggtgctttt tattacttta tgtaccctgt ttaacctgcc
     2401 tttgtaacat tttaagcatc tgaaacgtct tgttattagt tctcagaaaa gtaaaaaacc
     2461 gataatttaa tagtatagtt taaattttta tgactgtttt tcaatatatt acaatttttt
     2521 atactactat gtgttgcata gtactgtttt ataaatatat ctttgcatcg caataatcaa
     2581 taaaaacata tacagatgaa acgcttcaaa attttaacca tcattttagt tgcagcaata
     2641 ttgcctgcat tagcacaaaa cacacaacca ccggtagtag ttcctgcatt gatcagtcag
     2701 ggcgaggtga taccctatca acaacttccg gaagtcatca ttaccccgaa tgctgctgaa
     2761 caaaaagctg ttgaattacc cgttgttacg attactgcaa ataaaattaa cgaacaaatt
     2821 tatcctgcag taaagtatgg caacaactac attgcctcag taacaatagc acctattaat
     2881 gtggtggcac atcagcagaa aagaaccttt gcatcactgt tctcatttct tcgctacttt
     2941 agccgttaac aaaacctctt attttgcttg ctgcttttat agcattcatt tttttacaaa
     3001 cataaaaatc aaatttctgc caatgaagct taccattaaa ggcctgacaa aaacatatcc
     3061 caacggagtg caggcattaa aaaatataaa ccttgaaata aagaacggcc tttttggttt
     3121 gttaggtccc aacggtgcag gtaaatcatc cctgatgcga acaatagcta cgctgcaaaa
     3181 tgccgatagc ggaaatatat ttttagatga cctaaatgta cttgaagata aagaagaagt
     3241 aaaaaaaata ttgggctatc tgccacagga atttggtgtt tatcctaaca tatcagcaca
     3301 agagctgctc acgcatcttg ccttacttaa aggtgttcat attaaaaaag agcgcgatga
     3361 agttgtgaaa agtttgttaa tgcaaacaaa cctttatgag gtgcgcaaca aaaatttagg
     3421 tggttttagc ggtggaatga aacagcgttt tggtattgct caggcattaa tcggcaatcc
     3481 aaaaattatt attgctgatg agccaacggc aggtcttgac ccaacagaaa gaaaccgttt
     3541 tctgaatttg cttagcgaaa tcagcgagca ggtaattgta atcctttcta cacacattgt
     3601 tgaagacgtt aaagagttat gctctcaaat ggctattatc aataaaggtg aagtaatgtt
     3661 cagtggtcac cctgcagaag caataaaaca agttcagggg aaaatctgga tgtgccaggt
     3721 tgacaagcct acagctcgtg agctgatgat aaaatataac gtcatttcac aaaaattcaa
     3781 taccggcctc attgaactgc atgtctggag cgaagaaccg cttaatcatc acttcgccat
     3841 tgcgcagccc gttttggaag atgtttattt ttctgttctt aacggtaatt taaaacctta
     3901 agctatgtgg agtgtactca gttttgagtt gtcgtaccgt ttcagaaggc ctgcaactta
     3961 catctattta gctattttat ttctgatcag ctttttattc actgctacag atgcaatttc
     4021 tatcagtggt gctgttggca acattcataa aaactcacca tacaccattt atcaggttat
     4081 gttgattctt actcttttag gtacgatgat tatctctgcg gtgatgggtg ttcctgtgta
     4141 tcgcgactat gaacatcgct ttcatgaaat tatgtttacg cttcccgtta caaaagcaca
     4201 atatcttgga gggcgttttt taggttcata tttcactgcc ttatttattt ttctgggaat
     4261 accattaggc attttaacag gattattaat gccatggatt gacaaagaag ttataggtcc
     4321 gtttatgctg aatgcctatg tacttccctt tctgtacatc atcataccca atgtgtttat
     4381 tctgggtgta atgttttttg ctacaggctc acttttcaga aatcaattgg caatttatct
     4441 gcaagggatg gttttcttta ttctttattt agtgcttacc tctgcaaccg gagatgtaga
     4501 aaataatcca aaatttgcct tactcgatgt attcggcatc acaccatcat tttatattac
     4561 acgctactgg acaccatttg aaaaaaatct gcaaacaata ccattggaaa gttatctgtt
     4621 gtggaatcgt ttgttgtggg tgggcattgc tttaataggc gcttttgtta cttatcggtt
     4681 gtttcagttt tcaaagagca gcccgtttgt tttgcgcagc agaaaaaatc aggaaaaaat
     4741 tagtgttgaa aatctaagtc acatcaacct tccagaaact tcaaaagatt actccttttc
     4801 aacaaaatta cggcaatggc ttcaaatggt gcgattcgat ttttttggca ccatacgctc
     4861 tgttcctttt attgcaatct ggatttgtgg agtgattctt atacttggtc aaatgcctca
     4921 aataggcaaa atgtttggca ctacgatata tcctgttacc tatgctatca ttgatgattt
     4981 gaccggaaat ttcaccttgt ttactctgat aattattact ttttattccg gtgagttggt
     5041 gtggaaagaa cgagaacgcg gcattcatct gataacagat tcacttcctg tttcaagata
     5101 tgcaatgata acagccaagt tttttgccat gtgtcttatt gtggccatgt tgcaattgac
     5161 tgtcatgttt atgggaattt tcattcagac cttaaaagga ttttatcgct atgatattga
     5221 tgtttattta aaaacacttt ttgtcagtca gtacccaagt ttgattttac ttgtgttgct
     5281 tgcatttttc atccacagca tggtcaacaa taaatttatc ggccacacct tgatgattgc
     5341 ctattttgta ggaagaatgt cagcggccgc tatggggata acgcataaaa tgatttggta
     5401 tggtaacatt ccttctgcac cctactcaga catgaatgga tatggacatt ttctttatcc
     5461 taaatatatg ttcttaattt attggggaag cttaggactt gtacttttca ttattggtgt
     5521 aatgatgaat cgtcatggta gtgaaacagg aattaaagcg cggtggcaaa tttttaaaca
     5581 acaatggaaa ttcagcagag gaagaaaaat tgtatggtca ttacttttag ttttcattgt
     5641 ttcgggatgt tttgtctttt acaataccaa catccttcat aaatttaaaa gcgataaaac
     5701 tgtcagacat gagcaggccg aatacgaaaa acgatttaga aaatatcttg attttaatca
     5761 gccgcgcata acagcagtga atgtaaatat tgatttggtt cctgaaaaga gatacgctgc
     5821 cattaaaggt tattatatca tacaaaacaa atctgcactg cctattgata catttttaat
     5881 aacctgggca catgagaata taaaaatcaa caggtttggt tttgatattc tggctgatga
     5941 agttgaaaat tatcagaaac agtttgtcgt ttataaatta gctcatgcat taaagcccgg
     6001 tgactctaca aaagtttatt tcgacattga atacatcaac aaaggctttg ccaattcaga
     6061 agaggagtta gggttggtaa acaacggtac ttttatcaat aacaactatt tgcctgcatt
     6121 tggatattca gaatcgtttg agttgaccga caacagcgac agaaagaaag aagatttgcc
     6181 aagaaaaaat cacaccatgc gtttgctcag tgatagtgca tcgcgcaaca acacttattt
     6241 agctaatgat gcagactgga taagatatga gagcatcatc agcacatcac ccgatcagat
     6301 tgccgtttca ccgggatacc ttcaaaaaga atggacacaa aacggaagac gttatttcca
     6361 ttataaaatg gatgcaccaa tggtgaattt ttattctttt ttaagtgcac gctatgaagt
     6421 ctttcgtgat tcctttcagg gaataaacat tgaaatattc tatcacaaaa cacattcgtg
     6481 gaacattgaa aaaatgaaca aagccattaa aaaaacattg gcttactgtc agaaaacttt
     6541 ttcaccttat cagttccgtc aggttcgtat tctggagttt ccacgctatg aaacctttgc
     6601 tcaatcgttc ccaaacacca ttccattcag cgaagccatt ggttttattt atgatgttag
     6661 cgaagacacc gatatagaca atgcctttta tgtaacctca catgaggtgg cacatcagtg
     6721 gtggggacat caggtctgtg gtgccaacag tcagggttca actttgatgg tagaaagtat
     6781 ggcgcaatac acagcactga tggtgatgga aaaagagttt ggaaaagatg ccatggaaaa
     6841 atttcttaaa tacgaattgg atggctattt atcaggacgt tcatcagaaa gaataaatga
     6901 gcaacctatt gcacgcaacg atggacaggg atatattcac tatcaaaaag gtagtttggt
     6961 aatgtatgca atgaaagact atttaggcga agacacacta aatgcagcct tacacagatt
     7021 tataaaaacg tatggatttc agtccccacc ttatgtacaa gcaccgcagt ttgttcaata
     7081 cattgcagat gcaacacctg acagtttaaa gcaagtggtt gaagatatgt ttaaccgtat
     7141 cacgctgttt gatttaaaat gtgttgaagc cggctacaaa aagaaaggca acaaatatga
     7201 ggttacctta gatgtagaga ccggaaaagt ttatgcagac agtatgggaa atggaacagg
     7261 ggttaaacca aatgactgga ttgatatagg cataatgaaa tatgatggca acggaaaaca
     7321 ctcttatcaa tatttgcaga aacaattatt tacaggtggc agaaaaacaa ttgttgttac
     7381 tgttgatgaa aaaccgtcta aggcaggtat tgacccattg cataaactga ttgacaggaa
     7441 tagcgaagat aacatcaagg aagtaaaatc cttacaataa tttttctgac attgacattt
     7501 actttttatc ttggcagcca tgcttgaggt aaaaaatctg aaagttgtat ttcatcaaaa
     7561 cagtaatcct gttgttgctg taaatgattt ttctttttct attgcagcag gcgaaacctt
     7621 aggattggtt ggcgaatcag gttccgggaa aactgtttcg tcattggcat tacttggatt
     7681 gttgaacaat aaaaatgttg ctataagtgg caatgcatct ttcagtttac aacaacctgt
     7741 tgatctttta acgctaaacg aaaaacaatt aacagctatc agaggtcgtc atattgcaat
     7801 gataatgcaa gagcctatgc aggcatttaa tcctctgttt acttgtgggc atcaggtgac
     7861 tgaagctatt cgaaaacaca ctgcattaaa caaaaatgat gcaacagaaa aagtattgca
     7921 acagttcgaa aaagtaaagc taccaaatcc attacaaata agtaagcgtt ttcctcatca
     7981 actttcagga gggcagctac agagagttgc cattgcaatg gctataagtt gcaatccatc
     8041 acttctaatt gctgatgaac caacaaccgc cttagacgcc actgtgcaaa aagacattct
     8101 tttattgcta caacaattac agtcagaaaa taacatgagc atgcttttta tcagtcacga
     8161 tcttgaagca gtaaaattga ttgcacataa cacattagtg gttcatgcag gacagatggt
     8221 tgagcaggaa aagacagaaa aaatatttgc acatcctgca caagaatata caaaagagct
     8281 attgcaaagc cgtccttcag gattcaaaag aaacagtgtt atcagtgaaa agaatattct
     8341 gtcagtagaa aacatcagca aaagttacaa ccaacggcaa gggctattac gcagtaaaca
     8401 aaatattata aaagctgttg acaatatcag ttttacagta aagcaagatg agactttagg
     8461 cattgccggt gaatcgggat ctggaaaaac aacattagct aaattattac tgcgacttat
     8521 tgaaactgat agtggcgtga taaaattaag cgaagaaaat cttaaagact accctattcg
     8581 caccaaacaa ttatggcgaa aaaaaattca ggcagtattt caaaatccac atggctcact
     8641 cgatccggag atgactgtgg gcagacaact tacagaagtc tttaaattac atttttcatc
     8701 acaaaatacc actactgtta aaaaccagat tattgaatgg tttgaaaaag taggattaac
     8761 agaacaacat tttaaccgct atccacatga attaagttcc ggacaaaagc aaagactgtg
     8821 cattgcccgt gctctgatac cacaacccga tttacttatc tgcgatgaag ctgtgagtgc
     8881 acttgatgtt tctgttcagg caaaaattct tgaattgata aacaggctgc aacagcaatt
     8941 tcatttcgga tgtattttta tttcgcatga cttgaaagtt atccgtcaga tgtgtgacaa
     9001 tgttttggtg atgaaagatg gcaaagccga ctgttattca gcaactgaaa aactatttga
     9061 gaaacctcct acaacctatt cagagatatt attaaaatct attatttaaa aaatgattaa
     9121 catacgcaaa ggaacacctg cagatgttga acctgcactt cgtcttatca aagaattggc
     9181 agaatatgaa aaagcgccag aacaggttat caatactgtt gagcaaatgc ttaaagatgg
     9241 ctttggagaa aaaccgattt ttgatttgtt agttgcagaa accaatgaca atatagtggg
     9301 tatagcaatt tactttatca aatattcaac atggaaagga aagtgtcttt atttagatga
     9361 tattgttgtg caagaatcat tacggggaaa aggtataggc cggaaattgt ttgatgctgt
     9421 tgcagcagaa gccaaattac aaaactgcca acagttgcaa tggcaggtac tgaactggaa
     9481 cgagcctgca attaattttt ataaaaagta cgatactgtt tttgatgatg aatggatcaa
     9541 ttgtaaaatt attctgtagt ctttctttgt tacattcgta cagacaaatt caattgcaca
     9601 aaacacattg tcattaaatc agtatttcac tgccaaacca ggaaaagcat cagcaataaa
     9661 ggcagattgt ttttgtttaa ccacaatggc agattgtttg taaggtaaaa cacatgcatc
     9721 cggaagtccg taaaattttt aaaagcatta cagtaccact cctcctatca cttctaatga
     9781 taggtgtaaa aatttttgaa gtcttatcgc atactgattt aggtgagtac ggtgttttgc
     9841 caagacaaat aagcggatta aaaggcattt tgtttattcc atttcttcat gccgactggg
     9901 aacatttgtt cagtaatgtt gttccgctgt tagttacagg cagtatgtta ttttactttt
     9961 ataaagaaat ggctgtaaag gttatactgc tcatttacat tttaagtggc ttttggcttt
    10021 ggcttggtgg acgagaaaat tatcatattg gtgccagcaa tattgtttat ggcatcacag
    10081 catttttatt tctaagtggt tttatcagaa agcatacagg gcttattgct gtttcattgc
    10141 tcatggtttt tttatacggc agtttaattt gggggctttt tcctgtggtt ccaaaaattt
    10201 catgggaagg gcatttattt ggtgccctgt ccggaatact ttgcgcaata gttttcagaa
    10261 aagaaggtca gcagaaggaa gtttatgagt ggaacgatga tgaacccttt gatgaccaag
    10321 aaaatgatga taatgcaaca cagtcatcat taaaaattgt ttattcgtat aaaaaatcag
    10381 acgaaaagaa tggcactgaa gaatcttgaa cacattggta ttgcagttaa aagtctgcaa
    10441 atttctgatg ctttatttga agcattaata ggttgtccgc cttataaaaa agaaagcgtt
    10501 gaaagcgaaa atgtaattac ctcattcttt cagacgggcg aaacaaaagt tgaactttta
    10561 gaagcaactt caccggaaag tgccattgca aaatttattg ataaacgtgg tgaaggaatt
    10621 caccacatag cctttgctgt tgatgatatt aaagcagaaa tgcaacgact gcaacaatca
    10681 ggctttacac ttttaaacag tgagcccaag aaaggtgccg acaataaatt ggtttgtttc
    10741 cttcacccta aatcaactaa tggtgtgttg gtagaattgt gtcaggaaat taagtcttaa
    10801 aaagcagaga gcattgccat cagttctttt tcaatttcca tggcattgtc atgatgaatg
    10861 gcattcatac ctaccttttg tgcacctaat gcattgttca gattatcatc aataaaaacg
    10921 gtttcagcag gagttacacc actgtctttt atcacatcag caaaaattct tttatcaggc
    10981 ttgcgtgttc ccatctgaaa agaataatat gttttttcga acagtttctc aaagcggtct
    11041 tttccaaaag tctgattaag actcttgata aatgcagcat gatgaataat gttggtatta
    11101 ctcaaaaggt aaatattata cttatcacga agctcactca gaaaattaaa tctctcggga
    11161 ttcagttcac caagcaaggc attccatgca taatcaattt cttcatcaga aagcggacgt
    11221 ttataatgct ttcttatttc gctgcgaaat gcagaggcag ggataagtcc cttttcgaaa
    11281 tcatcaaaaa acttggcttg cttttgcatt gaatacagtt cgtcaaaatt gtccaacccc
    11341 atttttccaa actccaaaac ggttttttct gtatttatcg gaagtaacac cccaccaaaa
    11401 tcaaaaatga tatttttaat cattctcttg ctatcttttg tttattgcgt gcaaaaatat
    11461 acttttgcag ccctcaaagt tcatttaact tataattctt ttcgggccca tagctcagtc
    11521 ggttagagca acagactcat aatctgtggg tccctggttc gagcccaggt gggcccactt
    11581 tctttagctc gataaaaacc acactttatt aaaaattctt ttttcctttc tgaagttaag
    11641 tttcaagaat aatacctttg tcatcatttt tataatatgg ttgcacgcca cgatccgtta
    11701 aacaaaataa cacttggagg acttttagtc accttgggaa ttatctatgg cgacataggt
    11761 acatctcctc tctatgtaat gaatgccatc attggcgaca gacccataag tcctgaatta
    11821 gtacttggag gtatcagttg tatattctgg acattgacac ttcaaacaac ttttaaatat
    11881 gttttcctga cactgcgtgc cgacaacaaa ggtgaaggtg gaattttttc actctatgca
    11941 ttagttagaa aaactaaggt aaagtggttg gctattgctg caatacttgg aggcagcgcc
    12001 ttacttgctg atggattaat cacccctgcc atctctgttt cgtctgctgt agaaggtatt
    12061 cagattcttc atccagacct acctacaata cctattatta ttaccataat ctttggtttg
    12121 ttttttattc aacagtttgg cagcaacttt gttggaaaat cttttggtcc tatgatgctg
    12181 atttggtttt ccatgttatt tgttttagga tttcatcaac tcattcagaa cccgtctgta
    12241 cttaaggctt tcaaccctta ctatgcctat cagttacttg taaattatcc tggaggcttt
    12301 tgggtccttg gtgcagtttt tctttgtact acaggagcag aagcacttta ctctgacctt
    12361 ggtcacgtag gacgtaaaaa tatttatatt acctggacaa tggtgaaatt ttgcttgttg
    12421 atcaattatt ttggtcaggg tgcacacctg ctaaagtttg aaggaaagac aattgatgta
    12481 aatccatttt accaacttat gcctgattgg tttctgttaa taggcatcat tatttctact
    12541 cttgcagcaa ttgttgccag ccaggcatta atcagtggtg catttaccgt agtaaacgaa
    12601 gccatgcgac tgaactttgg cccaaaactt aaagttgttt atccaacaga cctcagaggg
    12661 caagtatata ttccaaccgt taactggact ttatgtgcag gatgtattgg cgtaatacta
    12721 ttttttcaac actcatccaa tatggaaggt gcttatggat tagcgataat cattgatatg
    12781 atgattacaa caattctgct ttcattctat ttgtatttaa aacgttatcc gctaccgtta
    12841 gtaatgctta catttcttct ttttattgtc attgaaattg cttttctggt ggccaactcc
    12901 attaaatttg aacatggcgg atatgttacc atgctactta ctatgttttt attcatcacc
    12961 atgtatgttt ggtatgcagc acgtaaaatc agaaatcagt ttgttgactt tgttaaggta
    13021 aaagattttt tagagatact taccaacctg agtaaagacg aatctattcc taaatatgct
    13081 accaaccttg tgtatcttac cagtgccaac cgcagagatg aaattgaatc gaaagtgatg
    13141 tattcgctct tacagaaatt tccgaagcgt gccgacatct attggtttgt tcacgttgat
    13201 gtgcttgatg aaccttacac catggaatat aaggttgcag aaatggtaaa tgataaggtt
    13261 atccatattg atttcagact tggtttcaga atagagccac gaatcaatca gatgttccgt
    13321 caggttgttg aagagcttgt aaaaaataat gaggttgata ttaccagtcg ctacacttca
    13381 cttaaagaaa aaaatgtgat aggtgatttc cgttttgtag tgattgagaa aacactatcg
    13441 tatgacaaca aactgaattt ctatgaaaag tttgtgctca atgcttatga ctttttaaaa
    13501 ttatttagtt tgcccgagtc aaaagccttt ggtcttgaca ccagttctgt aactgtagaa
    13561 aacgttcctt tagtagtaaa accttcaggt aaatttaaac tcaaacgaat ttaatcgttt
    13621 gtaattttat tgttgggata acgcaccaag tgaatacgtt ttactttttc gagtaacatg
    13681 gttcgcagtt cagaaatatt atctttctct gtagctgaaa taaatattgt ttctacattg
    13741 cctttattca tccacgtctt tcgtaaatca tctaatgaaa tattttcgct tgtcatgggt
    13801 gtcaggtcat ctgcttcttt ttcaatataa gtaaatgcat caattttatt aaagacaaga
    13861 attacaggtt tgtctgctga ttgaagttcc tgcaacatct gattcaccac atttatctgt
    13921 gcttcaaaat tgggatgcga aatatccact acatgaatta aaatatcggc ttcgcgcacc
    13981 tcgtcaagtg ttgacttgaa tgattcaact aaatgatgtg gcagctttct gataaatcca
    14041 acagtatcgc tcaacaaaaa aggaatattt tcaagcacaa cttttctgac tgtagtatct
    14101 aatgttgcaa agagtttgtt ttctgcaaac acatctgact tgctaagcag attcataatt
    14161 gtggacttgc ccacattagt ataacctacc aatgcaacac gcaccatatt acctcttgcc
    14221 ttacgttgat taaagccttg cttctctatt tcttttaaac gttcacggag tttactaagc
    14281 cggtcacgaa caatacgtct gtctgtttca atttcttttt caccgggacc gcggttacct
    14341 atacctccct gctgtttttc gaggtgtgtc cacatgcgtg tcaggcgtgg catcagatat
    14401 tggtattgtg cgagctcaac ctgcacctgt gcctgcgctg tgcgtgccct tcgtgcaaaa
    14461 atatcgagga taagttgtgt tctgtccaat acctttatct gtaatatttt ttcaatgttg
    14521 cgttgctgcg aaggcgacaa ctcatcatca aaaacagcaa ggtttatttc attcgctttg
    14581 ataaactcat ttatctctgc tagttttcct gaacctacaa aggtgcgtgg gtcggtatga
    14641 tctaactttt gtgtaaacga ttttaaggtc tgtgcgcctg cagtctctgc aagaaaagcc
    14701 aactcatcaa gatactccat ctcctgttct acaggctgat cttttaatat tacgcctact
    14761 aaaacggcac gctccttaac atcagtaagt ggcttgaaaa cggttttttc tttcatcaat
    14821 aaataaaact gaaataaatt atttcagtgt ctgaacatat tctgcaacgg cagcaatttg
    14881 ttcttgtgat aattgatctt tataggctgc cattgttttt ttgccattgg taatgatgtc
    14941 aattttttcc tgtggagata aagcagactg agtaaggtct tttgcaccac tcaatcctaa
    15001 ttttccatca gcaccatgac aagactggca tttactttca aaaataacct tacctgcagc
    15061 agcaccttca aggccttctg tatttacagc tgtatcagcc tttttcatgg atattgattt
    15121 ggtttcagaa atgccataag cataaatcaa tgccatgagt gaaatcaatg ccaatgagct
    15181 gtttttcttt ttaaatgcaa ttactgcaag tggaatagtg caggcaatga caaacaattt
    15241 aacccacaac catgtaccct tgttacctgt gttgacagcc agataaattc cggttagtag
    15301 gaacaatgtg ctgatgatca tttctgcaac cttagttttt ttaacaaagt tgtctaatgc
    15361 agcatgcctg ttcagcacta acaaggttgc tttaatgagg tataaaacaa taaaaagtga
    15421 aactaccagt gtgtgtgtgt gtaaaattcc tgtgtacata atgataaaga taaatgattt
    15481 ttgcggagag agagggattc gaaccctcgt tacacttttg gcgtaaacac actttccagg
    15541 cgtgctcctt caaccactcg gacacctctc ctttttgagc ctgcaaagct aacaaattaa
    15601 catgatggct aaagaaaaag cctgccgaaa aaaatacgac aggctgagaa cctaaaacca
    15661 aacctatctt ttgaccgttg tcaacagatt gattaaaacc gcatcaaaag taggttgaca
    15721 aattgaacaa ctgcgccaat gcgtagtttg ttatcaacac atttattcac gactaagttc
    15781 tcctgccata tccaattgca tattcacttt tatctcttga taatccattt cgctgcccaa
    15841 taaaaacata agtttggtaa tggcagcttc agtagtcatg tcatggccac tgactacacc
    15901 tgcacgtttt aacgcaagtc cattctcata cttttccatc tgcacaacgc ctccactaca
    15961 ctgcgaaaca tttacaatta ccatttgatt ttttactgca tcgcctaatt gctttagcag
    16021 tttttcattc ttaggcgcat tgccggagcc atacgtttca atgatcaatc cttttagcgc
    16081 acctgacttt aaaattgctg acaatacttt ttcatttact cccggaaaca atttaaaaat
    16141 catcaccgaa ttattgaagt gcgtctttac tttcagcgca ttgttgttaa cctcacgaat
    16201 ggcatcgtgg ttgtaattaa tttcagtgcc tgcctcagcc aatgacggat agttgatgga
    16261 ttgaaaagca tcgaattttg aagatgtaaa tttttctgct ctgttacctc tgaacaactg
    16321 attgttaaaa caaatacaaa cctccggcac cacaacattt ccacaggcaa tttcagctgc
    16381 tgtaataagg tttgattttg catctgttct gatttcgcca ataggtaatt gtgatccggt
    16441 gaggattaca ggcttgttca gattctgaaa catgtagctc aatgccgaag cagtatatgc
    16501 catagtatcg gttccatgta aaacaataaa tccatcatac ttgccataat tctcttcaat
    16561 aacttctgca atttgtatcc atcgttcagg ttgcatatca gagctgtcaa tcagcgggtc
    16621 gaatgaatga aagtctattt tacagggaag tttcttcaac tcaggtacaa cggtgctgag
    16681 cttgccgaag ttaaagggtt ttaacgatcc tgtctcttcg tcatgaatca tacctatggt
    16741 tccaccggta taaattacca gaaggttctt cttatctgtc tttatcattg tttgaaaata
    16801 actttagaat tgtttgtagt ctgttcagca acttctgcta ccgaacaatt ttttatgaca
    16861 gctaatttct gtgctgtcag cagtaaatat tcaggaatat tccttttgcc acgatgaggc
    16921 accggaggta aatatggcgc atcggtctcc aaaacaatgt gctttaatcc tacctgactg
    16981 acaattgcat caagacctga attcttgtaa gtaactacac ctccaatgcc cagatacatt
    17041 cccatgtcgg ttatagcttt tgcttcttct atacttccac tgaaacaatg gaaaacacct
    17101 tttacactta gacctgataa ttgtataagt tcaatggcac gtgtagttgc tgagcgcgaa
    17161 tgaatgctta ccggcagatt taattcacaa gaccatgtaa tctgttttaa aaaagcttct
    17221 tcctgttctt ttataaaact tttatcccag taaaagtcaa gaccaatttc tcctacacca
    17281 aaatattttc
//
LOCUS       DAOOOI010000045        17132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_674_length_17132_cov_17.226269, whole genome shotgun sequence.
ACCESSION   DAOOOI010000045 DAOOOI010000000
VERSION     DAOOOI010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17132
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_674_length_17132_cov_17.226269"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..1500
                     /gene="sucC"
                     /locus_tag="PKX59_06945"
     CDS             310..1500
                     /gene="sucC"
                     /locus_tag="PKX59_06945"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003007924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOZ90640.1"
                     /translation="MNIHEYQGKSILKSFGVAIQEGIVAETADQAVVAAKELQNQTGT
                     NFWVVKAQIHAGGRGKGGGVKLAKSIDEVKEKANAIIGMQLVTPQTGPQGKKVHKVLI
                     AQDVYYPGETKTAEFYMSVLLNRQAGKNIIMYSTEGGMDIEEVAAKTPEKIFKEEIDP
                     AVGLQPFQARKIAFNLGVSGNAYKEMVRFVTALYKAYESCDASLFEINPVLKTSDNKI
                     IAVDAKVNLDENALFRHADYAAMRDKSEEDATEVEASEHDLNYVKLDGNVGCMVNGAG
                     LAMATMDIIKLAGGEPANFLDIGGTANAARVEQAFRIILKDPNVKAILVNIFGGIVRC
                     DRVAQGIVDAYNNIGQIPVPIIVRLQGTNAEEAKKIIDQSGLKVFSAILLKEAAQLVK
                     EKLG"
     gene            1507..2400
                     /gene="speB"
                     /locus_tag="PKX59_06950"
     CDS             1507..2400
                     /gene="speB"
                     /locus_tag="PKX59_06950"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOZ90641.1"
                     /translation="MKALSTKHNFLGIEDEKYYNYESSKYVIQQVPYEHTSSYLAGSD
                     KGPAAIVKASQYVELYDEELDAETYKTCGIATLKALDFKNKTNKKAVDYIEKETLALL
                     NDNKFVVSLGAEHTVTLGFVKAHIQKFKNVSVLQIDAHSDLRESYHNNIYSHASVMKR
                     IFDLGLNIVQAGIRAQCIEEAQLIKKSKRLHTYYAHNIRKDAKWIDKAVKDLSENVYV
                     TIDADGFDPSVIPAVGTAEPNGLFWNETLELLKKTAKEKNIIGFDIVECAPTKGNILS
                     EYTLAKLAYRIIGYIEAARKE"
     gene            2420..3076
                     /locus_tag="PKX59_06955"
     CDS             2420..3076
                     /locus_tag="PKX59_06955"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOZ90642.1"
                     /translation="MENRKISNKGSATLFQNRLLERLTRTTFYFPVILFLLVGVATAL
                     YCYLSLKINVFIIVALFVAGLFAFTLVEYLIHRFVFHFNASTPGQELLKYKIHGVHHH
                     FPKDKDRLAMPPFMSLTLAVLFFLLYKWLFGNYGIALFSGFVSGYSVYLIIHYAVHRY
                     RQPRNFLSILWKHHSLHHYRSDNSAFSVSNPMWDYIFGTMPDKKSIQKGDKEKLPDSL
                     "
     gene            complement(3107..4894)
                     /gene="lepA"
                     /locus_tag="PKX59_06960"
     CDS             complement(3107..4894)
                     /gene="lepA"
                     /locus_tag="PKX59_06960"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOZ90643.1"
                     /translation="MENIRNFCIIAHIDHGKSTLADRLLEYTNTVSKQQMQAQVLDDM
                     DLERERGITIKSHAIQMDYTLDGKKYVLNLIDTPGHVDFSYEVSRSIAACEGALLIVD
                     AAQGIQAQTISNLYLALEHDLTIIPVLNKIDLPSAEPELVKDQIVDLLGCRREEIMAA
                     SGKTGMGVHDILREIVHRIPAPKGNPKAPLKALIFDSVYNSFRGIIAYFKVIEGEMKS
                     GDKVKFVATNSNYIAEEIGILKLDKIPAKTIGTGNVGYIITGIKDAKEVKVGDTITTL
                     ENPCSEAISGFEDVKPMVFAGIYPVDTEDYEELRSSMEKLQLNDASLVFEPESSAALG
                     FGFRCGFLGMLHMEIIQERLEREFNMTVITTVPNVSYVSYLTNGDVYEIHNPSDFPDP
                     SKMDHIEEPYIKAQIITKAEYIGAIMTLCINKRGVIIGQNYLTTDRVELMFEMPLAEI
                     VFDFYDKLKSVSKGYASFDYHPIGYKTSDLVKLDIRLNGEPVDALSALIHRDHSYTFG
                     KKICEKLKELITRQQFEIIIQASIGAKIIARETVKAIRKDVTAKCYGGDISRKRKLLE
                     KQKEGKKRMRQVGSVEIPQQAFLAVLKLD"
     gene            5043..5561
                     /locus_tag="PKX59_06965"
     CDS             5043..5561
                     /locus_tag="PKX59_06965"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HOZ90644.1"
                     /translation="MEELRYPIGKFQLPENITKSQFDSWKNDIAGLPKKVQQLTDSFK
                     SHHWSQTYRQEGWTALQLVHHLADSHINCFIRFKWALTEENPTIKAYDEKAWANLPDV
                     TQSSPVISIQLLHALHQRWINLINNMTEQDFEKTFFHPIQQKTHSLKGAVGLYAWHGN
                     HHLAHLKIIADK"
     gene            5646..7607
                     /locus_tag="PKX59_06970"
     CDS             5646..7607
                     /locus_tag="PKX59_06970"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ90645.1"
                     /translation="MIVLLLNFCTTQQKQTLPLLSSYASLNDTVKYVGKETCRSCHAD
                     IYNTFMQTGMGKSFDVASKQKTSAKFSHAVIYDKYENFYYHPYWQNDSLHLLEFRLEN
                     GDTVYKHNEVISYIIGSGQHTNSHIINTNGYLNQAPATFYTQRGEWHLPPGFEDGNNT
                     RFSRIIGLECMSCHNSYPDFVLGSENKYNKIESGINCERCHGPGEYHVQQKQAGILVD
                     TAKSIDFSIVNPAKLSVDLQFDVCMRCHLQGNAVLNNNKSFFDFKPGMKLSDVMNVFV
                     PVYEGNGNAHIMASHAERLKQSRCYIETANKNHANHQSSELRPGKNALTCITCHNPHV
                     SVRQTNDDHFNAVCKNCHHTENSLCTEKLSVREKSNDNCISCHMTFSGTSDIPHVSIH
                     DHKISIPSKDNKENEHKVFKGIYCVNNPTADKQSRGRAFIAYFERYGYDISVLDSALN
                     CFSDAGNMSLKENFNELVQIYFLKNDFAKVVALTSAMPDMLKMLNKKSYSNTHAWTAY
                     RIGASFQAIGNIEAALPYYQRAAVLAPYHLDILNKYATALSIAGNAKEAQKIYEFILK
                     EYPKHVSALTNLGFIFLSEKNDPQQALTYYSRALALNPDYEPALMNMAGLMHFMNRDG
                     EAVKFVQRILKINPRNEGARQLLNQLKST"
     gene            7608..8636
                     /gene="mltG"
                     /locus_tag="PKX59_06975"
     CDS             7608..8636
                     /gene="mltG"
                     /locus_tag="PKX59_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOZ90646.1"
                     /translation="MKKILFLILTLLLLAGAFVAYIFYQRVFAPNVKIKDNKSFLYIR
                     TGSNFNQVVDELTSQQILINAESFKWLAEKMNYTERVIPGRYEITDNMNNRQLLQLLR
                     SGKQVPIKLTFNNIRTKSDLASRISHQIEADSLQLIGMLNDSVLLKSESLNSENVLCL
                     FIPNTYEMYWNTDAADFFKRMKKEYDNFWTEIRKQKAAKQELTPVQVSILASIVQSET
                     KQNAEKPRVAGVYLNRLRQNWKLEADPTLVFALGDFSIKRVLNEYKTIDSPYNTYKYF
                     GLPPGPICLPDISSIDAVLNAESHQYMYFCAREDMSGFHAFAKDYNTHLLNARRYQAE
                     LNRRNIRN"
     gene            8670..9773
                     /locus_tag="PKX59_06980"
     CDS             8670..9773
                     /locus_tag="PKX59_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90647.1"
                     /translation="MRKGFLIVVAALFIAADVSADQVAALLRVKRSEQPDSLGFNLVE
                     QITKITYQLIQQNKIKLWDSPSKEIQITAASLQEIEQASSTSFKDEDVIYVYESWTEA
                     KREIQTKTIGISFSNRDSRNQEVSYGYVDYVDLARYLNEKPAEVNANGNYGETPAFYL
                     TAKKFNFSLVQFNNKVIQSADESQKTLRGFMGRSRFTVPPSDFSNEESKNIVYLVDRK
                     PGGDTLLSNNSSSFFNAVENYLLTNKEEFFNLGGDKVQNFVSGKQSLRVTSIEVNEVW
                     KKSSGRISYEPKSIRFYVNDSALNSIPLSEMTMMDLEINGQKLVLALLDKKFNFTITK
                     INSQDIALRQAFVYLKALREYHWNQLTEFVKYY"
     gene            9789..11204
                     /locus_tag="PKX59_06985"
     CDS             9789..11204
                     /locus_tag="PKX59_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HOZ90648.1"
                     /translation="MATKIKFGTDGWRAIIAQDFTVENVTRVAEATALWLKAKGGNPS
                     VVVGNDCRFAGNLFAETTAKVLCANGIKVMLAPDFVSTPMVSLGTLHYKADLGIILTA
                     SHNPPSYNGFKLKGSYGGPLLPAQVSEIEDMIKDASTIDTLKLSLEDFTAKGLLQIVD
                     LETMYINKVKENFDLDAIKKSGINWAYDAMYGAGQNVMKKLFPEITLLHCDHNPGFHG
                     QAPEPIHKNLTEFSELIKKRGDIGCGLVTDGDADRIGLYDDKGNFVDSHHIILLLIHY
                     LHKYKGMSGKVCTAFSTTPKVQTLCKHYGLPIDVVKIGFKYICEIMIKEDVLLGGEES
                     GGIAIKGHIPERDGIWMGLVIWEFMAKSGKSLNELIKEVYEITGPFSFERNDLHIKEE
                     VKQKILANCLQNKYAQFGNYKVQRVDDLDGFKYFFDNTNSEWLMIRASGTEPVLRTYA
                     ESSSREKAFQILDACKQTLLG"
     gene            complement(11402..12016)
                     /locus_tag="PKX59_06990"
     CDS             complement(11402..12016)
                     /locus_tag="PKX59_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ90649.1"
                     /translation="MIKVCIVEDIHEIRDGFKSVLDLSGEFKCCGTFGNAEDAIDKIP
                     ELNPEIVIMDINLPGINGIEAIKYLIKNNCNPLFLIFTVYDDDTIIFDALQAGASGYL
                     LKKTTPAQFLEAVKEMYDGGSPMSPIIARKVINSFKPGQKTNAILSVRESEILTLLAK
                     GLLYKEISEQLFISTGTVKQHIHKIYEKLHVQNRTEAINKFLNK"
     gene            complement(12062..13420)
                     /locus_tag="PKX59_06995"
     CDS             complement(12062..13420)
                     /locus_tag="PKX59_06995"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HOZ90650.1"
                     /translation="MQGDKLTYKYYHLPLNVLLGVLICMMLFSIVYFTLNHKKVFIYY
                     FLYLLFTFLYFFIRHHDNSVTSYYSFQLEPFRDITWQTLSYLFYFFFAISFVDYKNIS
                     PILYKTIQVIIYLIITYLIFDIFLYQNHFFEIRNKAYNIFRITMAPIAIIIILSAFLI
                     KSPLSKILATGSLLMVSGATVTMILSLFYYPSANPFINYHMLYMQLGIILEVICFTLG
                     LSYMSKIQFKEKIIIESQLNKIKENYEIDRLQTIINTQDNERKRVASELHDDLGAGLS
                     TIRLLSELALQKTEGKTEIKRISELSNELVDSMRQIIWSMNPESSSLSDFIHYLKRYT
                     MEIIELNGKIFNFKLNQEISDYTLSPEQKRNLFLIIKETLHNSIKHSKSNEIILIYDY
                     IEKRHHLTIKDNGIGFSPDDINYKNGNGLINIQKRAMQIGCSVFVTSEITKGTITRIE
                     FS"
     gene            complement(14057..15217)
                     /locus_tag="PKX59_07000"
     CDS             complement(14057..15217)
                     /locus_tag="PKX59_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90651.1"
                     /translation="MKQIRKKHFSVKLFTSVMAFILLMNHTTSAQIDCSSASGILALM
                     AAPVAINTVDPDDFPLNPSLIVSCTTKVFKTPPASISSICTNAIKMYVFYSTTQSDLS
                     SLPNLASSQLAAVLKKPNFKATITSTSNQNEKKVSVTFPNNKFIAGQRVYYRYGKLID
                     HPTDMDPMTWSPILNFITPQHIVNLPSACINPDLKPAPENMNEIILFKIIGGSWADAA
                     GHTYQQISGEWCKSFFNTSGVIIEPITGNDPSIGPMRQKTISLPPINWGVRETNNRPI
                     NNSFTNQLTRSGENTILATFNISSMTANSTQMGPPFTNRGTRTVYLFEQQNKACAVRT
                     FDAVHGNLNITENNYIVKVDSGNTITECTDSSNDNIKTYSHGGGNTVVVASN"
     gene            complement(15308..16537)
                     /locus_tag="PKX59_07005"
     CDS             complement(15308..16537)
                     /locus_tag="PKX59_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90652.1"
                     /translation="MRKFYSFLSILFCLNNFAHSQIIQTESPAKITPREINVASSSIF
                     DMMGVSSSQISKTSDVKDLKVDWSFKSWKLNPNIAVQGQPVWEIIYNRRDLSKYRSAS
                     YVMRTLASTDISAGTVQAENNDRRIGGAIKFNLYRKYDALRSEVYYKDIEQKYNTQFE
                     DWQQRMILLKSTIDTTTDIGKRSDLKMQISLLENEAALLNQSRKNEITSRATEFNAEN
                     WNSSFVDVAAGKIWTYSTDSAGTLKSVRINRQSGWAMWTNAGYGIGSKILITGLFRVS
                     FYDEQLNFLIKDLDTGNEITATSLVSNKIYSYGINFRYGTPSINFFSELLLEKRQLKT
                     VKDAFNKSDFVGEGNFEIIEGTLKWTALSPFTISFGGEWRISKNLLLNFGMRMEYEKN
                     WKRKTFQPIAGISCLMR"
BASE COUNT         5473 a         2827 c         3205 g         5627 t
ORIGIN      
        1 ataaaaatac tctgacatgc aaattgactt ttaggatttt ttttaaattg ttaaaatatt
       61 ttgcaaaata gataaaactt tgtttcttta caccacaatt cattgggaga tgaagcgatt
      121 agatgataat tttatctgga aagatttgag ttttcttcag gaattggggt taagcataat
      181 cctgattgcg atttttatct atttttttgt aagccaatat aacggctgat ttcataagtt
      241 caaatctttt tatttaacaa caaggcatca ttttgatgtt taagtttatt tttgtgaaaa
      301 tttttcataa tgaacattca cgaatatcag ggcaaatcca ttttaaaatc ttttggtgta
      361 gccattcagg aaggaattgt agcagaaaca gccgatcagg cagtagtagc agctaaagaa
      421 cttcaaaatc aaacaggaac aaacttttgg gtagttaaag cacagataca tgcaggtggt
      481 cgtggtaaag gtggaggtgt aaagctagcc aagtcaatag atgaagtaaa agaaaaagcc
      541 aatgcaatta tcggcatgca gttggtgaca ccacaaaccg gtccgcaagg taaaaaagtg
      601 cataaggtac taattgcaca ggatgtttat tatcccggtg aaacaaaaac cgcagagttt
      661 tatatgagtg ttttactaaa tcgtcaggca ggtaaaaaca taatcatgta ctccactgaa
      721 ggagggatgg atattgaaga ggtggcagca aaaacacctg agaaaatatt caaagaagaa
      781 attgatcctg cagtaggact tcagcctttt caggcaagaa aaattgcatt caaccttggt
      841 gtaagtggta atgcttataa agagatggtg cgttttgtta ctgcattata taaagcgtat
      901 gaaagctgtg atgcatcgtt gtttgaaatt aatcctgtat taaaaacatc ggacaataaa
      961 attatagctg ttgacgccaa agttaacctc gatgaaaatg ctttgttccg tcatgcagat
     1021 tatgctgcca tgcgcgataa gtcagaagaa gatgcaacag aagttgaagc cagcgagcat
     1081 gatttaaatt atgtaaagtt agatggtaat gtaggctgta tggtgaatgg tgcaggcctt
     1141 gccatggcaa ctatggacat tattaaatta gcaggtggcg aaccggcaaa ctttcttgat
     1201 attggaggaa ctgcaaatgc agcccgtgta gaacaggcat tccgtatcat tctgaaagac
     1261 ccaaatgtaa aagctatatt ggtaaatatt tttggaggta ttgtgagatg cgatagagtg
     1321 gctcagggaa ttgttgacgc ttataataat ataggacaga ttcctgttcc gattattgtt
     1381 cgcttgcagg gcaccaatgc cgaagaagca aaaaagataa ttgatcagtc gggactaaaa
     1441 gtgttctcag caattttatt aaaggaggct gcacaattgg taaaagaaaa gcttggataa
     1501 tagaatatga aagccctgtc aaccaagcac aattttttgg gtattgaaga tgaaaagtat
     1561 tacaactatg aatcttccaa atatgttatt cagcaagtgc cttacgaaca tacaagttcc
     1621 tatctggcag gctccgacaa agggccggct gctattgtaa aggcttcgca gtatgtggaa
     1681 ttgtatgacg aagagttaga tgcagaaaca tataaaacat gcggcatagc aacacttaaa
     1741 gctcttgatt ttaaaaacaa gaccaataaa aaagcagttg attatattga gaaggaaacg
     1801 cttgcgctgc ttaacgacaa taagtttgtg gtttcacttg gtgcagagca tacggttact
     1861 ttaggatttg tgaaagcaca tattcagaaa tttaaaaatg tttcagtgct tcagattgat
     1921 gcccatagtg acttgcgcga atcttatcac aacaatatat attctcatgc cagtgttatg
     1981 aaaagaattt tcgatctggg attgaatata gtgcaggcag gtataagagc acagtgtatt
     2041 gaagaagcac aattaataaa aaagtcaaaa cgactgcata cttattatgc gcacaacatc
     2101 agaaaagatg cgaagtggat tgataaagca gtgaaagatt taagtgaaaa cgtttatgtg
     2161 accattgatg cagatggctt tgatccatct gtaataccgg ctgttggaac tgcagagcca
     2221 aatggcttat tttggaatga aacattagaa cttcttaaaa aaactgccaa agagaaaaac
     2281 attatcggct ttgatattgt tgaatgtgca cccactaagg gaaatatttt atcagaatat
     2341 acattagcaa aactcgccta caggataata ggctatatag aagcggcaag gaaagagtag
     2401 gtaattaaaa caatagtgta tggagaacag gaaaatatca aacaagggtt ctgccacatt
     2461 atttcagaac cgtttacttg aaagactgac acgtacaact ttttattttc cggtaatttt
     2521 atttttgctt gtcggagttg caactgcgtt atattgttat ctaagtttaa agataaatgt
     2581 attcatcatc gttgcacttt ttgttgccgg cttatttgca tttacacttg ttgaatatct
     2641 gatacatcgt tttgtttttc atttcaatgc cagcacaccg ggtcaggaat tgctgaagta
     2701 caagattcat ggcgtgcatc accacttccc aaaagataaa gaccgtttag ctatgccacc
     2761 attcatgtcg ctaacattgg cagtgctgtt ttttttattg tacaaatggc tatttggaaa
     2821 ttatggtatt gcgctgttct ccggatttgt aagtggctat tcagtctatc ttatcattca
     2881 ttatgctgtt catcgttaca ggcagccacg aaatttttta agcatacttt ggaaacatca
     2941 tagcctgcat cactaccgtt cagacaattc agctttcagt gtttcaaacc ctatgtggga
     3001 ttatattttc ggcactatgc cggataagaa aagcattcag aaaggggata aagaaaaact
     3061 tcctgattcc ttgtaaagta attttctgaa tgtttagaaa aggaaattag tcaagtttta
     3121 aaacagctaa gaaagcctgc tgtggaattt ctacactacc tacctgacgc atacgtttct
     3181 ttccctcttt ttgtttttca agaagttttc tctttctgga aatatcacca ccataacatt
     3241 ttgcagtaac atctttcctg atagctttta ctgtttcgcg tgcaataatt ttagcaccta
     3301 tacttgcctg aattataatt tcaaattgct gacgggtaat cagctctttt aatttttcgc
     3361 aaatcttttt gccgaatgta tatgagtggt cgcggtgaat caatgcagaa agtgcatcaa
     3421 caggttcgcc attaagtctg atatcgagct ttaccaaatc actggttttg tatcctattg
     3481 gatgataatc aaacgaagca tatccttttg aaacagattt caacttgtca taaaaatcaa
     3541 aaacaatttc tgccaagggc atttcaaaca tcagctccac acggtctgtt gtaaggtagt
     3601 tttgacctat aatgacacca cgtttgttaa tacataaagt catgatggca ccaatatatt
     3661 ctgccttggt aataatctgt gctttaatat aaggctcttc aatatgatcc attttactgg
     3721 gatccggaaa gtcgcttggg ttgtgaattt catacacatc accattggta agatacgata
     3781 cataagaaac gttaggaacg gttgtaatca ctgtcatgtt aaactcgcgc tccaatcttt
     3841 cctgaataat ctccatgtga agcattccta aaaagccaca acggaaaccg aagcctaatg
     3901 ctgctgacga ttcgggttca aaaaccaatg aggcatcatt caactgaagt ttttccattg
     3961 atgagcgcaa ctcctcatag tcttcagtat ctacaggata tattccggca aataccatag
     4021 gcttaacatc ttcaaaaccg gaaatagctt ctgaacatgg gttttccaaa gttgtgatgg
     4081 tatcaccaac ttttacttct ttggcatctt ttattcctgt aatgatatag ccaacattac
     4141 ctgtgcctat agtctttgca ggtatcttat caagtttcag aataccaatt tcttcagcta
     4201 tataattact gttggtagca acaaatttta ctttgtcacc cgatttcatt tcgccttcaa
     4261 taactttaaa atatgcgatg atacctctga aagaattata tacagaatca aaaatcagtg
     4321 ctttaagagg agcttttgga ttgccttttg gtgcaggaat acgatgtaca atttcacgca
     4381 aaatatcatg aacgcccata cctgtttttc cactggctgc cataatctct tcacgtctgc
     4441 aacctaacaa atctactatc tgatctttca ccaattcagg ctctgcactc ggaaggtcaa
     4501 ttttgtttaa aacagggata attgtaaggt cgtgttcaag cgctaaataa agattactga
     4561 tggtttgcgc ctgaataccc tgtgcagcat caacaattaa taatgcacct tcgcaggcag
     4621 caatacttct tgatacttca taagaaaagt caacgtggcc gggagtatct atcagattca
     4681 gtacatactt tttcccatct aaagtatagt ccatttgaat ggcatggctc tttatggtga
     4741 tgccgcgttc gcgttccaga tccatatcat caagcacctg tgcctgcatc tgctgcttgc
     4801 ttacagtatt ggtatattca agcagacggt ctgcaagggt gcttttgcca tgatcaatat
     4861 gggcaatgat gcaaaaattt ctgatatttt ccattgggcg gcaaaaatag cttttttaag
     4921 ccttcatacc atggtttttt aaagtttgga agacatattt ttacaaatga aagatgcttc
     4981 tgttttcaga cttttgtcag aatattcaat aatgctaaat caagattaaa ataagacaaa
     5041 ttatggaaga actacgttat ccgataggaa aatttcagtt gcccgaaaat attactaagt
     5101 cgcagtttga tagctggaaa aatgatattg ccggtttgcc aaaaaaggtg caacagttaa
     5161 cagatagttt taaatcgcat cattggtcgc aaacgtacag acaggaaggg tggacagcct
     5221 tacagttggt gcatcactta gctgatagcc atataaattg ttttatccgt tttaagtggg
     5281 cattgactga agaaaatcct acaattaaag cctatgatga aaaagcatgg gctaaccttc
     5341 ctgatgtgac tcaatcatca ccggtgattt ctattcaact acttcatgca ttgcatcaga
     5401 gatggataaa tcttatcaat aatatgaccg aacaagattt tgaaaaaact ttttttcatc
     5461 ccattcaaca aaaaacacat agcctgaaag gcgcagttgg gctttatgca tggcatggaa
     5521 atcatcacct ggcacatttg aaaattattg ctgataaata aatttgttaa tggtttattg
     5581 ctgatgattt ttggttttta tttgtgttta tgaggcaaaa actcatttct ttttccattc
     5641 ttttaatgat agtgttattg ttgaattttt gcactacgca acaaaagcaa acactgccat
     5701 tgctttcatc ctatgcaagt ctgaacgata cagtaaaata tgttggcaaa gaaacctgcc
     5761 gttcatgcca tgccgatatt tacaatacgt ttatgcaaac gggcatgggc aaatcatttg
     5821 atgttgcttc caaacagaaa acatcagcta aatttagtca tgcagtaatt tatgataagt
     5881 atgaaaactt ttactaccat ccctactggc agaacgattc attgcacttg ctagaattca
     5941 gacttgaaaa tggcgatact gtttataagc acaatgaagt tattagttat attatagggt
     6001 cgggtcagca taccaactca catatcatca acaccaacgg ttatttgaat caggcgcctg
     6061 caacttttta tacacaaaga ggtgagtggc atttgcctcc cggatttgaa gacggcaaca
     6121 acacacgttt ttcacgaata atcggtttgg aatgtatgag ttgtcataac agttatcctg
     6181 attttgtttt gggttcagaa aataaataca ataaaataga gagtggcatt aactgcgagc
     6241 gttgtcatgg cccgggcgaa tatcatgttc agcaaaaaca ggccggtata ttggttgata
     6301 ccgctaaatc aatagatttt tctattgtga accctgccaa attatctgtt gacctacaat
     6361 ttgatgtatg tatgcgttgt catctgcaag gtaatgctgt attgaataac aacaaatcat
     6421 ttttcgattt taaaccgggc atgaaactga gtgatgtgat gaatgtgttt gttcctgttt
     6481 atgagggtaa tggtaatgca catatcatgg catcgcatgc cgaaaggctt aaacaaagca
     6541 gatgttatat agaaacagcg aataaaaatc atgctaatca tcaatcatct gaattaagac
     6601 cgggaaagaa tgcattaacc tgtattacct gtcacaatcc gcatgtcagt gtgcggcaaa
     6661 ctaatgacga tcatttcaat gcagtttgca aaaattgtca tcatacagaa aatagtttgt
     6721 gcacggagaa gttaagtgtt agagaaaaat caaatgataa ttgcattagc tgtcacatga
     6781 cattttcagg cacatcagat attccgcatg tcagcataca cgatcataag ataagtattc
     6841 catcaaaaga caacaaagag aatgaacata aagtgtttaa gggaatttat tgtgtcaaca
     6901 atcccacggc agacaagcag agtaggggaa gagcctttat tgcttatttt gagagatatg
     6961 gttatgatat aagtgtactc gactctgcac taaattgttt ttcagatgca ggaaatatgt
     7021 ctctcaaaga aaactttaat gaattagtac agatatattt tctgaagaat gattttgcaa
     7081 aagttgttgc actgacttcg gcaatgcctg atatgttgaa gatgttgaat aaaaaatcgt
     7141 attctaacac acatgcctgg acggcctatc ggataggtgc atcttttcag gcaattggaa
     7201 atattgaagc agcattgcct tattatcaac gtgccgcagt attagcccca taccatctcg
     7261 atattttaaa taaatatgct actgcacttt ctattgcagg taatgctaaa gaggctcaga
     7321 aaatatatga gtttattcta aaagagtatc caaaacatgt ttcagcattg acaaaccttg
     7381 gttttatttt cctctctgaa aaaaatgacc cacaacaagc attgacatat tatagcagag
     7441 cattggcact taatcccgat tacgaacctg cattaatgaa catggcaggg ctaatgcatt
     7501 ttatgaatag agacggagag gctgttaagt ttgtgcagcg tatcttgaaa atcaatccca
     7561 ggaatgaagg cgcacgacaa ctattaaacc aactgaaatc aacataaatg aaaaaaatac
     7621 tttttttaat tttaacttta cttttattag caggtgcttt tgttgcatat atcttttatc
     7681 aaagagtatt tgctcccaat gtaaagatta aagacaataa atctttttta tatatcagaa
     7741 caggaagtaa ttttaatcag gttgtagatg agttgacatc gcaacaaatt ttaatcaatg
     7801 cagaatcatt caagtggctg gcagaaaaaa tgaattatac agaacgtgta attcccgggc
     7861 gctatgagat aactgacaat atgaacaaca ggcagttgtt gcaattattg cgctctggaa
     7921 agcaggttcc cattaaactt acctttaata atatcaggac caagtcagac cttgcctctc
     7981 gtataagtca tcagattgaa gcagacagtt tacaattaat tggtatgctt aacgacagtg
     8041 tactcttaaa atccgaatcg ttaaatagtg aaaatgtttt atgtctgttt attcccaaca
     8101 cttatgaaat gtactggaat actgatgctg ctgatttttt caaacgtatg aagaaagaat
     8161 acgataactt ttggacagag atacgaaaac aaaaagcggc aaagcaagaa ttgacccctg
     8221 tgcaggtgag catattggca tctattgttc agtcagagac aaagcaaaat gcagaaaaac
     8281 ctcgtgtggc aggagtttat cttaacaggc tcagacaaaa ctggaaattg gaggcagacc
     8341 caacacttgt ttttgcttta ggtgattttt cgattaaacg tgtgctgaat gaatataaga
     8401 ctattgattc gccttataat acttataaat attttggact gcctccgggt ccaatttgtt
     8461 tacctgatat ttcaagcatt gatgctgttc tcaatgctga aagtcatcag tatatgtatt
     8521 tctgcgctcg tgaagatatg agtggttttc atgcttttgc aaaggattat aatacgcacc
     8581 tgctcaatgc cagacgctat caggctgaat taaacagacg taacattcgg aattaattca
     8641 ctgtaaaacg gtatttttac gccaataaaa tgagaaaagg gtttttaatt gttgttgcag
     8701 cactttttat tgctgctgat gtttctgccg atcaggtggc tgcactactt cgtgtaaagc
     8761 gtagtgagca acccgattca cttgggttta atttagttga acagattacc aaaataacct
     8821 atcaacttat acagcagaac aaaattaaat tgtgggactc accatctaaa gaaattcaaa
     8881 ttacagctgc atcgttacag gaaattgaac aagcatcatc aacttcattt aaagatgagg
     8941 atgttattta tgtatatgaa agttggactg aagctaaacg tgaaattcaa actaaaacaa
     9001 taggaataag tttttcaaac cgcgacagcc gcaatcagga agtatcctac ggttatgttg
     9061 actatgtcga tttagctcgc tatttgaatg agaaaccagc ggaagtaaat gctaatggta
     9121 actatggcga aacacctgca ttttatctca cagcaaaaaa atttaatttc agtttggtgc
     9181 agtttaataa taaagtaatt cagagtgcag atgagtctca gaagacatta agaggtttta
     9241 tgggaagatc ccgatttact gttccaccat cagattttag taatgaagag tctaagaaca
     9301 ttgtttattt agttgacaga aagcccggag gagatacttt gttatcgaac aatagcagta
     9361 gtttttttaa tgcagtagag aattacttat tgaccaacaa agaagagttt tttaatttgg
     9421 gtggtgataa ggtgcaaaat tttgttagcg gcaaacaaag ccttagggtt acttcaattg
     9481 aagtgaatga ggtatggaag aaaagtagtg gacgaatttc ttatgaacct aagagtatcc
     9541 gtttttatgt taatgattct gcattaaata gtattccact ttctgaaatg actatgatgg
     9601 atttagaaat caatggtcag aaattagtcc ttgccttgct tgataaaaaa tttaatttca
     9661 caataacaaa aattaattca caggatattg cactaagaca ggcttttgtt tatctaaaag
     9721 cacttcgtga gtatcactgg aatcaactaa cagaatttgt aaaatattat tgaacattat
     9781 aaaaatttat ggcaacaaaa attaaattcg gtaccgatgg atggcgtgca atcattgcac
     9841 aagattttac agtagaaaat gttacacgtg tagcagaagc tacagcactg tggctaaagg
     9901 caaagggtgg taacccttca gtagttgtag gcaacgattg tcgttttgca ggaaatctct
     9961 ttgctgagac tactgcaaaa gttttatgtg caaacggaat taaagtgatg ttggcaccgg
    10021 attttgtttc aacgcccatg gtttcgctag gtacattaca ttacaaagcc gacttgggaa
    10081 ttattctaac agcaagtcac aatccaccat cgtacaatgg atttaaattg aaaggttctt
    10141 atggcggacc gttattacct gcacaggttt cggaaattga agatatgata aaagatgctt
    10201 caaccataga cactttaaaa ttgtctttgg aagattttac tgcgaaagga ttgctacaga
    10261 tagttgacct tgaaacaatg tatatcaaca aggttaaaga aaactttgac cttgacgcta
    10321 taaaaaagag tggaattaac tgggcttatg atgccatgta tggtgctggg cagaatgtga
    10381 tgaaaaaatt atttcctgaa attaccttgt tgcattgcga ccataatccc ggatttcatg
    10441 gtcaggcacc ggagccaatt cataaaaacc tgacagagtt ttcggagttg ataaagaaga
    10501 gaggtgatat tggttgcgga ttagttactg atggcgatgc cgaccgtata ggactttatg
    10561 atgataaagg aaattttgtt gactcacatc acatcattct gctgcttatt cattacctgc
    10621 ataagtacaa aggaatgagt ggaaaagttt gtacggcatt ttctaccaca cctaaagtgc
    10681 agacgctatg taagcattac ggtctgccca ttgacgtagt taagattggt ttcaaataca
    10741 tctgcgaaat aatgattaaa gaagatgtat tgttaggtgg agaagagtcc ggtggtattg
    10801 ccattaaagg acatattccg gaacgtgatg gtatttggat gggattggta atttgggagt
    10861 ttatggctaa gtcaggaaaa tcactgaatg aacttattaa agaagtatat gaaataaccg
    10921 gtccattttc tttcgaacga aacgatcttc atatcaaaga agaagttaaa caaaaaattc
    10981 ttgcaaactg ccttcagaat aaatacgcac agtttggcaa ttacaaagta caacgtgttg
    11041 atgaccttga cggatttaaa tatttcttcg acaataccaa cagcgaatgg ctgatgatcc
    11101 gtgccagcgg aacagagcct gttctcagaa cctatgcaga atcatcttcc agggaaaaag
    11161 catttcagat tttagatgct tgtaagcaga cactgttggg gtagtgccat atattacttg
    11221 cgtgaagtgc tcaaagttat cttgtaagat ttttttacta aacgtgcgta aagttgttta
    11281 cttaacggct gccgtcttac ataaattaca gcagactttg gggggtattt aattttttat
    11341 aatttaatag aaaattatac gcaagttcta atcggctcag gttttgaaat acgttctact
    11401 ttcatttgtt caaaaactta ttaattgctt cagttctatt ctgaacatga agcttttcat
    11461 aaattttatg aatatgctgt tttactgttc cggtactaat gaaaagttgt tctgatattt
    11521 ctttatataa cagtcccttt gcaagtaatg ttagaatttc agattcacgt acacttaaaa
    11581 tggcatttgt tttttgccca ggtttaaatg aatttatcac ttttcgtgct ataattggac
    11641 tcatcggtga acctccatcg tacatctctt taactgcttc cagaaattgt gcaggtgttg
    11701 ttttttttag tagataccca cttgcacctg cttgcaacgc atcaaatatt attgtatcat
    11761 catcataaac ggtaaaaatt agaaacaatg ggttacagtt gttttttatt aaatacttaa
    11821 ttgcttctat tccatttata ccaggtagat tgatgtccat tatcacaatt tctggattta
    11881 actcaggaat tttatcaata gcatcttcag cattgccaaa tgtgccacaa catttgaact
    11941 cacctgaaag atccaagaca ctcttaaatc cgtctcttat ttcatgtatg tcctcaacaa
    12001 tgcatacctt aatcatgttg caatattaat tatttgaggg gtaaattaga tataactttt
    12061 gttaactaaa ttcgattctt gtgatagttc ctttagtaat ttctgaagtt acaaatacag
    12121 aacagcctat ttgcattgct cttttttgaa tgttaattaa accattcccg tttttgtaat
    12181 taatatcatc gggagaaaat ccgataccat tgtctttgat agttaggtga tgcctttttt
    12241 caatatagtc gtatataaga atgatttcgt tagatttaga atgcttaatg ctgttgtgca
    12301 aagtttcttt tattatgaga aataaatttc ttttttgctc aggactaagt gtataatcag
    12361 aaatctcttg atttaattta aaattaaata tttttccgtt taattctata atttccatcg
    12421 tatatcgctt taaataatga ataaagtctg ataaactgct gctttcagga ttcatgctcc
    12481 agattatttg tcgcattgag tcaacaagtt catttgataa ctcactgatt cgttttattt
    12541 cagtttttcc ctcggtcttt tgaagagcta gttcgctcag taatcgtatg gttgaaagac
    12601 ctgcacctaa atcatcatgt aattctgagg ctactctttt tctttcatta tcctgtgtgt
    12661 tgattatagt ttgcagtcta tcaatttcat agttttcttt tattttattc aattggctct
    12721 ctattattat tttttcctta aattgtatct ttgacatata gcttaatcct agtgtaaaac
    12781 aaattacttc caatattata cctaattgca tatataacat atggtaatta ataaatggat
    12841 ttgctgatgg ataataaaat aatgatagaa tcatggttac cgtagcaccg cttaccataa
    12901 gtaaactccc tgtcgctaaa atttttgaca gaggactttt tattaaaaaa gcactaagaa
    12961 ttatgattat tgcaattggg gccattgtga ttctgaaaat gttataggct ttatttcgaa
    13021 tttcgaagaa atgattttga tataaaaaaa tatcaaaaat caaatacgtg atgattaggt
    13081 agattattac ctgtatggtt ttgtaaagta ttggagaaat gtttttataa tctacgaaac
    13141 ttattgcgaa aaaaaaataa aacagataag ataaagtttg ccaggtaata tctcgaaagg
    13201 gttctaattg aaaagagtag taggaggtaa cagaattgtc gtgatgacgt ataaaaaagt
    13261 ataaaaaagt aaaaagtaaa taaaggaaat agtatatgaa aacttttttg tgattaagtg
    13321 taaaatagac aatggaaaac aacatcatac aaattaagac gcctaataaa acatttaatg
    13381 gtaaatgata atatttgtaa gtgagtttat cgccttgcat taatttaaaa agaacatctt
    13441 cggttttgga ggagctttct gtgtttataa ataacaaaag tttgttaaat ggcttgtagt
    13501 gtccagatat atttatccat agcttatatg aagaattagg cataaaggtt aatggaaaga
    13561 taaaggaatt gctttttgtt actacgtttt tcagataata atattctaga tcccctaacg
    13621 attcattaaa taggtagatt gatgatgaag ggaaggctcc tatataaaag tatctggtag
    13681 ttttatgtgc tgaattattc ttccattctg aatatatcca gatgcctttt atagatttat
    13741 ccttaatagt ataaggtata ttaatcttta catcggattg taattttgga aagaaattct
    13801 gatcagcaac ttgattgaag tgtagtgtta aacttgaatc atagaataag ctgcaacgaa
    13861 aatagggttc tttcaagtca tcagatatca ggtttgactc acctacatta gacttagtat
    13921 atccgttacg gcttaatatc agacaagtaa atatgaagaa acaaatatga gtgattcgca
    13981 tttttgtaat gcgaatgtaa ttgaaaaaat aacaggtaat gatttctcat tacctgtttc
    14041 attgagttaa ttttaattaa ttacttgcaa ccacgactgt gttgccccct ccatgactgt
    14101 aagttttaat attgtcattt gaactgtctg tacactctgt tattgtattg cctgagtcca
    14161 cttttacaat ataattattt tctgttatgt tcagatttcc atgaacagcg tcaaatgtac
    14221 gtacagcgca ggctttattc tgctgttcaa ataagtaaac cgttcttgtt cctctgttgg
    14281 taaagggagg ccccatttgg gtggaatttg cggtcatgga agatatatta aatgttgcca
    14341 gtattgtgtt ttcacctgat cgtgtcaact gatttgtaaa ggaattattt atagggcggt
    14401 tatttgtttc tctcacaccc cagtttatag gtgggagaga tattgttttt tgacgcatag
    14461 gtccaatgct tgggtcgttt cctgttatcg gttctatgat aaccccagat gtgttaaaaa
    14521 aagatttaca ccattctcct gaaatttgtt ggtatgtgtg accggctgcg tcagcccaac
    14581 ttcctccgat aattttaaat aatattattt cattcatatt ttccggtgct ggctttaagt
    14641 caggattgat acaagcggat ggtaaattta ctatatgttg aggtgtaata aaatttagaa
    14701 taggactcca tgtcattggg tccatgtctg tagggtggtc aattaatttg ccatatctgt
    14761 aatatactct ttgtcctgca ataaacttgt tgttaggaaa ggtgacactt actttttttt
    14821 cattttgatt agatgtcgat gttatagttg ctttaaagtt gggttttttt aaaactgcgg
    14881 caagctggct tgatgctaaa tttggcaaac tagaaagatc ggactgtgta gtagagtaaa
    14941 aaacatacat cttaatggca ttagtacata tggatgaaat actagcaggt ggagttttga
    15001 atactttagt agtacagcta acaattaagg acggatttaa tgggaaatca tcagggtcaa
    15061 cagtgttaat tgcaacaggt gctgccatta atgctaaaat tccagatgca gaactacagt
    15121 ctatttgggc ggatgtagta tgattcatta gcagtataaa agccattaca gaggtaaaca
    15181 actttactga gaaatgtttt tttcttattt gtttcatctt aatatgtttt aatttgattg
    15241 ttatgaacta ttgcaaatgt tcagcccttt tttaaatagt gctatagaac ttaagttagt
    15301 ttttatatca tcgcatcaga catgatattc ctgcaattgg ttgaaatgtt tttcttttcc
    15361 agtttttctc atattccata cgcatcccga agtttagtag taaattttta ctgattcgcc
    15421 attcaccgcc aaaagaaatt gtaaatggtg aaagggctgt ccattttaat gtgccttcaa
    15481 tgatttcaaa gttgccttca ccgacaaaat cacttttatt gaaggcgtct tttacggttt
    15541 ttaattgccg tttctccaat aaaagttctg agaaaaaatt gatagatggt gtaccatacc
    15601 taaaatttat tccataggag taaattttat tgcttactaa tgaagttgct gtaatttcat
    15661 tccctgtgtc taaatccttg attaagaaat ttaattgttc atcataaaat gatactctga
    15721 ataggcctgt aataagtatt ttgctcccta ttccatatcc ggcattagtc cacattgccc
    15781 atcctgattg tcggtttatt cgtacagatt ttaatgtgcc agcactatca gttgaataag
    15841 tccatatttt cccagccgca acatctacaa acgaactatt ccaattttca gcattaaatt
    15901 ctgttgctcg cgaagtaatt tcgttttttc tggattgatt taataatgca gcttcgtttt
    15961 ccagaagtga aatttgcatt tttaggtcag atcgtttgcc aatatctgtg gttgtatcaa
    16021 ttgtactttt taatagtatc atcctttgct gccaatcttc aaactgagta ttatattttt
    16081 gctcaatgtc tttataataa acctcacttc ttaaggcatc atatttccgg tatagattaa
    16141 actttatagc tcctccaatt cgtctatcat tattttctgc ctgtacggtt cctgcagaaa
    16201 tatctgttga tgctagggtt cgcatcacat aactagccga acgatattta cttaaatctc
    16261 tgcggttata aataatttcc catactggct gaccttgtac tgcaatgttc ggatttaact
    16321 tccaggattt gaatgaccaa tctactttta aatcttttac gtctgaagtt tttgaaattt
    16381 gtgaggagct tactcccatc atgtcgaaaa tggatgatga tgccacgttg atttcacggg
    16441 gtgtgatttt agcaggtgac tctgtttgga ttatttgtga atgagcaaag ttgtttaaac
    16501 aaaatagaat tgataagaaa gaatagaatt tacgcataac taatcagatt aaattattga
    16561 cagtaaatga gtaaaattcg cacagtaagt tctaatgtac aatataactt aggttagttt
    16621 gttttcgggt aatctctact cacataccaa aaatagttca tcgtgtgaca tatagttttt
    16681 caactacact ttgaggaata cttttttacg ttaagttgtt gaacgaatta tctcttggta
    16741 gtcttagtgt gaaaatagca gagtttaact atcttaaagt agctttgatt tatagtaata
    16801 agacaaattt ttctgtacca atgtttttca acttcttgcc gatttgagat tttgattctt
    16861 gataattttt gcagtaaaac tctgacataa atattgttct tttattggta ctcatcagct
    16921 gaatgtaagc atagaattgt tattcagcag gtggaggatc tgtcttgcat ccccatgtat
    16981 ttctgtgatt agaaatgttt tgtcatgacc acatcaccaa agtttcttgt cttgatttta
    17041 tcttgttcta tagaaatttt gatagccttt gttcttagtt caccctagac aaaccagatt
    17101 tgaaaaatag cactttaggc atattagttg ag
//
LOCUS       DAOOOI010000046        16968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_681_length_16968_cov_13.235913, whole genome shotgun sequence.
ACCESSION   DAOOOI010000046 DAOOOI010000000
VERSION     DAOOOI010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 16968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16968
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_681_length_16968_cov_13.235913"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PKX59_07010"
     CDS             <1..517
                     /locus_tag="PKX59_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ90653.1"
                     /translation="ILVDGKPLKEENLEYWRGLIGYVKQDIFMIDSNIKENVAFGEET
                     INNDLLNAAIKQASLNEFVGNLPKGVESEVGERGSRLSGGQKQRIGIARSLYRNAQIL
                     VFDEATSALDTQTEREVSEAIDSLSDTNKTIFIIAHRVTTLKNCDRIYELKNGEIAGV
                     YSYSELIEKVI"
     gene            527..1669
                     /locus_tag="PKX59_07015"
     CDS             527..1669
                     /locus_tag="PKX59_07015"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOZ90654.1"
                     /translation="MIKRLISHIRLRYLNLLSQSTAENFSDEQRLLMALEWLKQTLLP
                     NGGSAAKYSMMFNKWLPAYPETTAFWINTLIYLKNNKPELLQEVFNDRPVIDELIQWL
                     LSTQRKDGTFPGSYGDFKNQPPRVFNNGQIIMALTDYYQQSQKKEVLEAAIQSADWLL
                     KVQDSDGAWREFTLHQLSSNTRTAWALIKLGQHLNEPKYISAGINNINFALKQINNHD
                     YFCDNGFDENEIPTTHTIGYALRGVLGAGIDLQKKEWTEIVERSYKHIVALTKQNGFL
                     AGELDEEWTSDNAFCCLTGNCQLSVIGFVLFEQTAEIQYLETANLLLEYVKSKQLISH
                     SPMVNGGISGSWPVNGGYSAYDIPNWAAKFFADAMLLQMKHKTMKH"
     gene            1746..2846
                     /gene="wecB"
                     /locus_tag="PKX59_07020"
     CDS             1746..2846
                     /gene="wecB"
                     /locus_tag="PKX59_07020"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOZ90655.1"
                     /translation="MGPDQIKVTQFKKVNADLGMPFDIKIVHTGQHYDSKMADIFFEQ
                     FNLTPDFWLNIPAGSPNSQMAEIMLRLEKVIQENKPDLLMVVGDVNSTFAAALTANKM
                     DLKIAHVESGLRSFDRTMPEEINRLLTDEITDYFFITEDSGYKNLLKEGKHEKQMFFV
                     GNTMIDTLVAFDEKIQQSNILETLNIKSKEFVLMTMHRPATVDHKDGLKQLFSIIELI
                     TKNHQLVFPIHPRTLHRIEEFGMKSFITENKKLILTEPLDYLAFQKLISDCAYIVTDS
                     GGIQEESTFRQIPCLTLRANTERPCTVDIGTNELVPFSQEKISERIDAIVHNKYKKGV
                     IPPLWDGQSTKRILEACMKFLSSESHASALLK"
     gene            2901..3854
                     /locus_tag="PKX59_07025"
     CDS             2901..3854
                     /locus_tag="PKX59_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOZ90656.1"
                     /translation="MNNDAPFQFLPGFTHLYHEYMEENIFIAYSEKLMAYMPLRFFSS
                     RFFKLAQILHAPIKNNIELNPDEQLEFFNELISHLNQNNSCERLVQPHPYGILASVPA
                     NSRFCEFGTYIIDLQSQTLEEIFQKFHPKYQKAIHHSEKNGAVVKFGQEVIEDFYLCY
                     TDTMKRINMPSEELQFFKSYYNYLGSDNVTAGVVYDHDNPIGGIFMIHSNYAALCTHA
                     GSRGESKLYGSMKYLHSKMIERMKETGVNKYDLVGVRIGNNDPTLEGVFRFKRGFGGE
                     LKKGFLWKTDINPLKAKMYDLLIKIKHPNNKYKDIIDQITN"
     gene            3861..5033
                     /locus_tag="PKX59_07030"
     CDS             3861..5033
                     /locus_tag="PKX59_07030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ90657.1"
                     /translation="MHILIIPSWYKSITEPVLGTFFEEQARALMSAGHKVGIIYPQFS
                     SVSSMFNKKDEIIDFYDDNGLPTYSVIHQAIIPKMRKLAYKMFGENVEKIYHSYVKKY
                     GKPDLIHAHSVFHGGMAGYYIAKKNKLPFIITEHLTAYITGGITHQEDVETARQIFEN
                     ANAAIIVSENFKNDIEKALNLPKTTFTVIHNMVADLFFKDIVLKKYNQQEEFVFFTNS
                     FLLPRKNHKLLLDSFKYITAKGINNLRLRIGGDGPLYNELRDYVNELSLTGKVDFLGA
                     LSRTEVKRELDNCHSFVLSSNYETFGVVLIESLACGRPVVTTESGGPSDFIHSRNGIT
                     SSTSIDSLANAMTKMMEEYNTFDQRALSEECRDRFAEKKIEAEIEALYLTAINKHK"
     gene            5055..6101
                     /locus_tag="PKX59_07035"
     CDS             5055..6101
                     /locus_tag="PKX59_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90658.1"
                     /translation="MKTTKQVLITFDYELFLGNRSGSVSDCLINPTNHVLDVIEQYGC
                     RAIFFVDTTYLLTLKKNFQIAACKKDFETVMNHITEIASRGHEIFPHLHPHWLDAKYL
                     SATNEWQLLNTDKYRLYHINESERSEIFDQSIEILQQRISKVNPSYLVDSYRAGGWCI
                     QPFNTFLPYFIKHNIKFDFSVLGGFYLFSNAQYFDFSKAPQKPIYQFENDITVEQNNG
                     CFTEFNISSIYIPQQIKLFEKLFLKLYYKITGDHSFSRGEGQIVVKIDKALATPQQEG
                     HDILDSRWERIAIELMSAVKQGEYKKYLDANNYMHFISHPKMLTRHNIRMFDKFLKFA
                     SDKYNLESDFRKMV"
     gene            6134..7237
                     /locus_tag="PKX59_07040"
     CDS             6134..7237
                     /locus_tag="PKX59_07040"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90659.1"
                     /translation="MKIGSYIFELLHQHDCVIVPQFGGFVARYKNATIHPVQHLFSPP
                     SKIVAFNSELTTNDGLLANYISQKLLLSYSDSCKLINDFVTLCKAQLEANQRIQIEKI
                     GSLFFDPEKNLQFIPADNENFLTDSFGLSTVQSPAIQRDRPFEIFSNRDAAVQPAPKR
                     TNHSKLILKIAAYTGVAALIAFAYFNPAINSKISKGLATIFPVTTEIDSQANHSSVNN
                     NQEQKSKEDLIYSESPVSEKNIYETEKPTVDSSASQITAPTPEKEIAAVEEEPKPEPI
                     VETKKIAETVPAVSNATRQYHIVGGCFSVYDNALNLDAELKSKGFSSALIGKNKNGLE
                     IVAIASVATKSEAEEALQQAQNSGYKDAWIMRK"
     gene            complement(7904..8428)
                     /locus_tag="PKX59_07045"
     CDS             complement(7904..8428)
                     /locus_tag="PKX59_07045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOZ90660.1"
                     /translation="MITLIAGTNNKNSHTEKLARIYYQAFLDAKVEVNYLSLTLLNQY
                     IINEDMYDKNDSKINQIGDKYFLNSDKLLFVVPEYNGSFPGILKLLIDAMDVKKYLYF
                     KKAAVVGVATGRAGNLRGIDHLTSILQHTKITVMPHILPVSSVHLEIDEAHEFKNEKT
                     KMALQQHLKQFLAF"
     gene            complement(8438..10384)
                     /gene="dnaG"
                     /locus_tag="PKX59_07050"
     CDS             complement(8438..10384)
                     /gene="dnaG"
                     /locus_tag="PKX59_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOZ90661.1"
                     /translation="MIPKETVELIIETSRIEEVVGDFVSLKKRGANLLGLCPFHNEKT
                     PSFTVSPAKGIYKCFGCGKAGDSVRFMMEHEAFTYPEALRYLAEKYKITIEETIPTAE
                     EIQKQDERESLFVLYSFAQKFFSEKLFNTEEGRAIGLSYFNERGFNEAVIEKFQLGYA
                     PDQWDALSQAVIENKFNRQLYEKSGLAVVKEKEQDGQKVYDRFRGRVIFPIHNLSGRV
                     IAFGARQLVNDPKSPKYINSPETDIYHKSKSLYGISFARKAIIQHDECFLVEGYTDVV
                     SLFQAGVENVVASSGTSLTPEQIRLIARFTGNITILYDGDPAGIKASLRGIDLILEEG
                     LNVKVVLFPDGDDPDSFARKNSTTQLMEYISKNAVDFIGFKTGLLLKEVANDPVRKAE
                     LIRDIVESISRIPDGIKRSLYTSQCAAQMDVPEQVLITELNKIRRNLYKKDNEHQQAE
                     LVEKLIEMEYLPQQTILTETNAWYQEQEIIRLLLNYGDKKFNFQGSEKPTTVREFIIE
                     QILPDGFGFDDEIFAGVFSHLTSMSTTDFLSMLKTENSSELEKLRSKAHELLMNKYEL
                     SDWESKGIYVKTEENNFNEAIVNSINHIKLKRVQILKRKNSLLLKEEASLSDERLVEI
                     LSQQKIYEEYIYAFSKLLGIVTLE"
     gene            complement(10381..11373)
                     /locus_tag="PKX59_07055"
     CDS             complement(10381..11373)
                     /locus_tag="PKX59_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90662.1"
                     /translation="MLRKVIVVSLICAGSLFLLLYFTSLFSISELIYPNRITVKSLTA
                     EFHNLKKDELVIDKDVIVSPSQLNLNYRNFDVAIHDHFTLHGWYISGEQPATTTVLLL
                     HDWNESKLSMIKTAEALYQLGFNVCVVDLPAHGESDGEKFIIDQSMNHCLEVVADSLY
                     CLYETDNIAVVAYGLSGIIAASWIKVDSRSHCMILVDPVPGMIAKINELVKEKWNGFS
                     MLVTPFAQIKYRQKTGIDADSLNLQEYLGHLRTPLLVAISDSATDLEIIQSVRIFEEA
                     KSPNKKIWTNQTRSFIRIDTDAEKNMYRAMAAFINSNISSEKPKIKVRKRIAAL"
     gene            complement(11377..12354)
                     /locus_tag="PKX59_07060"
     CDS             complement(11377..12354)
                     /locus_tag="PKX59_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOZ90663.1"
                     /translation="MSLSLDQIKKPISLEMDCFENKFRDAMRSKVSLVDKIMHYIVKR
                     KGKQMRPMFVFLSAGVCGKINESTYTAASLIELLHTATLVHDDVVDDALQRRGFFSIN
                     AVWKNKIAVLVGDYLLSRGLLIAVQNKEFKLLEIVSNAVRDMSEGELLQMEKARRLDI
                     DEEVYYKIIRQKTASLIASCCSCGAASVQAGDEKVELMRELGETIGLAFQIKDDLFDF
                     EKSISTGKPSGIDIRERKLTLPIIYALSQATSSEKRTIINLIKNEHENESKVAEVLAF
                     VNRQKGIDYAREKMFELRSHAFEIINSFPDSDYRKSLHDLITFTTDRNY"
     gene            complement(12405..13469)
                     /gene="rlmN"
                     /locus_tag="PKX59_07065"
     CDS             complement(12405..13469)
                     /gene="rlmN"
                     /locus_tag="PKX59_07065"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOZ90664.1"
                     /translation="MFSSYLCKVKSIPNIRELTFEQLSEAVADFGEKSFRAKQIYDWL
                     WNKSARSFDQMNNVPLSLRNKLAEKFAFTPIVVAHEQRSNDRTIKYGFKLHDDHLIEG
                     VLIPTASRMTACVSSQVGCSLSCKFCATGKLDRVRNLNADEIYDQVAIIREQSMEVYN
                     IPLSNIVYMGMGEPLLNYANVLASIERITSANGLGMSPQRITVSTAGIAKMITKLGDD
                     EVKFNLALSLHAANDSKRDKIMPINEHNNLEVLRDALKYFYDKTGTRVTYEFIVFNNF
                     NDTLKDAQELAEFAKHVPCKINLIEYNAIGDGVFEKARLDRLEVFKNYLESKNLVVNI
                     RRSRGKDIDAACGQLANKNQ"
     gene            13503..14708
                     /locus_tag="PKX59_07070"
     CDS             13503..14708
                     /locus_tag="PKX59_07070"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOZ90665.1"
                     /translation="MKFYSADIVFPICSTPLKDGIVVMDNSGVVIDVFENIAFLNNAL
                     QIQIEPSVIEKFHGILTPGFINSHCHLELSHLKGRISKHNKLTGFIKELISVRKQGEN
                     EEVLDAIKSAEKNMFNDGIVAIGDISNTSITFPIKNESDLLYHTFIEVFDLSELNTEK
                     TWNTAKELEGMLKSNNRNSKCSISPHSPYSVTPFLFQKINKNKASLISIHNQETESEN
                     ILFETGSGDLKSFFEATGNNLVHIKANGKNSIQNYVPLLNNCDEIIFVHNTFSSDDDI
                     QFAIKNVKKAWWCFCPNANLYIENTLPEIERITKATENILIGTDSLASNDQLSILSEL
                     KIITNKFPQITLHQLLTWSTINGAKALKMDNELGTLEKGKKPGLVLIENVDLQNQKIT
                     ESSKSKKVA"
     gene            complement(14710..15258)
                     /locus_tag="PKX59_07075"
     CDS             complement(14710..15258)
                     /locus_tag="PKX59_07075"
                     /inference="COORDINATES: protein motif:HMM:NF026080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HOZ90666.1"
                     /translation="MKIKLIITSLIVAFSVSQINAQAVSYPVDNDSKLICYTKVKELT
                     GVSKNDIFDRALGWINSYYKNPADVLRENDKANGKLLCKARFKLMGEPDKSGFQKDQG
                     NVQYSLIIEAKDGKYRYKLTEINWKQPSYFPVERWMDTKNQYYTKDWDWNLKYTDEQS
                     HKIIAELEKYMSNPPKPKKDDW"
     gene            complement(15282..16844)
                     /locus_tag="PKX59_07080"
     CDS             complement(15282..16844)
                     /locus_tag="PKX59_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOZ90667.1"
                     /translation="MINLQNTFPLFSKKLINAMFIMPLVFLMLFVSNVWAGGNDTLSA
                     NIDENVHVAEKPKDFEIALQPALLSDNSTSQIRAGLIYDCTTNTIVWEKDLKYAYPIA
                     SLTKMMVALIAIEEIKCGTVDWQDQVSVTKTYRKSKRSKRTYTVNETYSLESLLKMAM
                     IPSNNEACGLIGKHISGSVETFVTRMNQKAAALGMTQTFYSNPSGLPAGYGSLDNSSS
                     PYDLLKLSTELIKYPELLSITNIAFAEVENGHGYNVYRNHNHLVMDYPGEVDGLKTGY
                     TKNAKFCLVATAKKNDHRLIAIALGASNPWLRNQIVGEMLNNYYTQLGFGPMGNQKVD
                     PLFAKADRKTVNEIASSNVPVMNQEIVYKTVTSIQKKAHIVKSGQTLSDIADKYNCSV
                     NELKKWNGLKSSRILKNQRLYVRVHVKKQIPVSTKVIDNYDDCEDNAEDCAPQNVAGK
                     QETPKTEQLPTSQNKPALKVIYHQVQPGDTLWKIAQQYPGTSVDQLKRINKISNSKDL
                     KAGSKIKVSVNS"
BASE COUNT         5618 a         3123 c         2809 g         5418 t
ORIGIN      
        1 gattttagtt gacggcaaac cattaaaaga agaaaatctt gaatactggc gcgggttaat
       61 cggctatgtg aagcaagata tttttatgat tgattcaaac ataaaggaaa acgttgcttt
      121 tggagaagaa acaatcaata acgatttact taatgctgca atcaaacaag catcattaaa
      181 tgagtttgtt gggaacctac ccaaaggagt agagtcagaa gttggagaaa gaggatcaag
      241 actatctggt gggcaaaaac aaaggattgg cattgcacgc tcgctttata gaaacgcaca
      301 gatacttgtt tttgatgagg caaccagtgc attggacaca caaacagaac gcgaagtaag
      361 tgaggccatt gactcacttt cagataccaa taaaacaatc tttatcatcg ctcaccgcgt
      421 gactacacta aaaaactgcg accgcattta cgaactaaaa aatggggaaa ttgcaggagt
      481 atattcctac agcgagttaa ttgaaaaagt aatttaattt ttaacgatga tcaaacggct
      541 gatttcacac ataaggttgc gatatctcaa tttattatcg caatcaacag ccgaaaattt
      601 ttctgatgaa caaagattac ttatggcact tgaatggctc aagcaaacac ttttaccaaa
      661 tggaggaagt gccgccaagt attcaatgat gtttaacaaa tggttacccg cctaccctga
      721 aaccactgct ttttggataa acactcttat ctatctgaaa aataacaaac cggaattatt
      781 acaggaagta tttaatgata gacctgttat tgatgagtta attcaatggc ttttatctac
      841 tcaacgtaaa gacggtacat ttcccggatc gtatggagat tttaaaaacc aacctcccag
      901 agtttttaat aatggtcaaa taattatggc gcttactgac tattatcaac aaagtcagaa
      961 aaaggaagtc cttgaggctg ctattcaaag tgctgattgg cttttgaaag tgcaagattc
     1021 tgatggtgca tggcgtgaat ttacgctaca tcaacttagc tcaaacacca gaacagcatg
     1081 ggctttaatc aaacttggcc aacatttaaa tgaaccaaaa tatatttcag caggaattaa
     1141 taatatcaac ttcgccttaa aacagattaa taatcacgac tacttttgtg acaatggttt
     1201 tgatgaaaat gaaataccaa ccacacacac tatcggttat gcattgcgag gtgtattagg
     1261 tgcaggtatt gatcttcaaa agaaagaatg gactgaaatt gttgagcgct cctataaaca
     1321 catcgttgca cttactaaac aaaatggctt tctggctgga gaattagacg aagaatggac
     1381 aagcgacaat gcattctgct gtctgacagg caactgtcag ttaagtgtta tcggatttgt
     1441 attatttgaa caaacagccg aaattcaata cttagaaaca gcgaatcttt tgcttgaata
     1501 cgttaaaagc aaacaattaa tttcacattc gccaatggta aatggcggca tttcaggatc
     1561 atggcctgta aatggtggtt attctgctta tgatatacca aattgggcag caaaattttt
     1621 cgctgatgcc atgctcttgc aaatgaaaca taaaactatg aaacattaga tattttaaac
     1681 tttataccta ccttcacgta taaaaagaca atagattttg agaatgaaaa agattttaat
     1741 tgtagttggg accagaccaa attaaagtaa cacagtttaa aaaggtgaat gccgacttag
     1801 gtatgccttt cgatattaaa atagttcata ctggccagca ctacgatagt aagatggctg
     1861 acattttctt tgagcaattt aatttaacac ctgacttctg gcttaacatt ccggcaggca
     1921 gtcctaattc tcaaatggct gagataatgc ttcgcttaga gaaagttatt caggaaaaca
     1981 aacctgactt attaatggtt gttggcgatg taaattcaac ttttgctgca gcattaacag
     2041 ccaataaaat ggatttaaaa attgctcacg ttgaaagtgg cctgagaagt tttgacagaa
     2101 caatgcctga agaaataaac cgtttattaa ctgatgagat aaccgattac ttttttatta
     2161 ccgaagacag tggctataaa aatcttctca aggaaggcaa gcatgaaaag caaatgttct
     2221 ttgttggaaa caccatgata gatacacttg tagcctttga tgaaaaaatt caacaaagta
     2281 atattcttga aactttaaat attaaaagta aagagtttgt cctgatgact atgcatcgtc
     2341 cggcaacggt tgatcataaa gatggtctga agcaattgtt ttcaataatt gaacttataa
     2401 caaaaaatca tcaacttgta tttccgattc acccacgtac actgcacaga atcgaagagt
     2461 ttggaatgaa atcatttatc acggaaaaca aaaaactaat attaacagag cctttagact
     2521 atctggcatt tcagaaactt atttcagact gtgcttatat tgtaacagac agcggaggta
     2581 ttcaggaaga aagtacattc agacaaatac catgtcttac attaagagcc aatacagaac
     2641 gaccatgtac agttgatatc ggaactaatg aattggtacc attcagtcag gaaaaaattt
     2701 ccgaacgcat tgatgcaatt gtacataaca aatataaaaa aggagttatt cctccattat
     2761 gggatggaca atccaccaaa cgaattttag aagcttgtat gaaatttctt tcaagcgaaa
     2821 gtcatgcctc tgccctattg aaataaacta tgttactact caaccaccta cagaaaaaag
     2881 acaagtcatt attggccgca atgaataatg acgcaccgtt tcagtttctt cccgggttta
     2941 ctcatttgta tcatgagtat atggaagaaa atatttttat tgcatactca gaaaaattga
     3001 tggcatatat gccattgcga tttttctctt cacgattttt caaacttgca caaatattac
     3061 atgcgccaat taaaaataat attgaactta atccggatga acaacttgaa ttttttaacg
     3121 aattaatatc gcacttaaat caaaacaatt cctgcgaacg attggttcaa ccccatcctt
     3181 atggaattct tgcctctgta cctgccaatt cgcgtttttg tgaatttggc acctatatca
     3241 tagaccttca atcacaaaca ttggaggaaa tctttcagaa gtttcatcca aaatatcaaa
     3301 aagctattca tcattcagaa aaaaatggag ctgttgtaaa attcggacaa gaagtgattg
     3361 aagattttta tttatgttat actgatacta tgaaacgaat aaacatgcct tcagaagagc
     3421 tacagttttt taaatcttat tataattatc ttggcagcga taatgttaca gcaggtgttg
     3481 tatatgatca tgacaatcca ataggaggta tttttatgat tcactcaaat tatgcagcat
     3541 tatgcacaca tgcaggaagc agaggcgagt ccaaactata tggttcaatg aaatatttac
     3601 acagtaagat gattgaacgc atgaaagaaa caggagtgaa taaatacgac cttgtaggcg
     3661 taagaattgg aaacaacgac cctacacttg aaggagtttt tcgctttaaa agaggatttg
     3721 gtggtgagct aaagaaaggt tttttatgga agacagacat taatccgcta aaagcaaaaa
     3781 tgtatgattt gcttattaaa atcaaacatc caaataacaa gtataaagac attattgatc
     3841 agattacaaa ttaatttttt atgcacattt taattatacc ttcttggtat aaatctatca
     3901 cagaacctgt tttaggaaca ttcttcgaag aacaggcgcg tgcactcatg tctgcaggac
     3961 ataaggttgg aatcatatat ccacaatttt catcagtaag ttcaatgttt aacaagaaag
     4021 atgaaatcat tgatttttat gacgacaatg ggcttccgac ttattctgtt attcatcagg
     4081 ctatcatacc taagatgaga aagctagctt ataaaatgtt tggagaaaat gttgagaaaa
     4141 tttatcatag ttatgttaag aaatatggta aaccagattt aattcatgca cacagtgttt
     4201 tccatggtgg aatggccggc tattatatcg caaagaagaa taaacttcca tttattataa
     4261 cagaacatct gacagcttac attacaggag gaatcactca tcaggaagat gtcgaaactg
     4321 ccagacaaat ttttgaaaat gcaaatgcag caattattgt aagtgaaaat tttaaaaatg
     4381 atattgaaaa agccttaaat cttccaaaaa caacttttac ggttatacac aatatggtag
     4441 ctgatttgtt ttttaaagac attgttttaa aaaaatataa tcaacaggaa gaatttgttt
     4501 tctttaccaa ctcattccta ttgccaagaa agaatcacaa attattactt gactcattta
     4561 aatacataac agctaaagga attaataatt tacggttacg gataggtggt gatggcccat
     4621 tgtataatga attaagagat tatgtaaatg agttatcact aacaggcaaa gtagattttt
     4681 taggtgcatt atcacggact gaagtaaaaa gagaattaga caattgccat tcgtttgtgc
     4741 tttcaagtaa ctatgagaca tttggtgttg tactcataga gagtttagct tgtggaagac
     4801 cggtggtaac aacagaatca ggcggaccga gtgattttat acatagcaga aatggaatca
     4861 cctcatcaac atcaattgat agtcttgcaa atgcaatgac aaaaatgatg gaagaataca
     4921 atacttttga tcaacgtgca ttatctgaag aatgtcgaga cagatttgca gaaaaaaaga
     4981 ttgaagcaga aatagaagca ctttatctga cagctattaa taaacataag taaagtaatt
     5041 ctgatcttaa aaatatgaaa accaccaagc aggtattaat tacttttgat tatgagttat
     5101 ttttaggcaa cagaagcgga tcggtttcag attgtcttat taatccaacc aatcacgtac
     5161 ttgatgtaat tgagcaatat ggatgcagag ccattttttt tgttgacaca acttatcttc
     5221 ttacactaaa gaagaatttt caaattgcag cttgtaagaa agattttgaa actgtaatga
     5281 atcacattac ggaaattgca agtcgtggtc atgaaatatt tcctcaccta cacccacact
     5341 ggctagatgc aaaatatctt tcagctacca atgagtggca attgttaaac actgataaat
     5401 accgcttata tcatataaac gaatctgagc gttctgaaat ttttgatcaa tctatcgaaa
     5461 tacttcaaca gcgtatttca aaggttaatc catcttattt agtagatagt tatcgtgcag
     5521 gcggatggtg cattcaacca ttcaatactt ttttaccgta ttttattaaa cataatatca
     5581 agtttgactt tagtgtatta ggtggttttt atctgtttag caatgcacaa tattttgact
     5641 tttcaaaagc accacaaaaa cctatctatc aatttgaaaa tgatattaca gtagaacaaa
     5701 acaatggttg ttttactgag ttcaatattt cgtccattta tattccacaa caaataaagt
     5761 tatttgaaaa acttttcctg aaattatatt acaagattac gggtgaccat agtttttctc
     5821 gcggtgaagg gcagatagtc gtaaaaattg ataaagcttt agctacaccg caacaagaag
     5881 gtcacgatat acttgactca agatgggaac gtattgcaat agaactgatg tctgcagtca
     5941 aacaaggtga atataaaaag tatttagatg ctaacaatta catgcacttt atctctcacc
     6001 caaaaatgct tacccgacac aatatcagaa tgtttgacaa gttcctaaag tttgcatcag
     6061 acaagtataa tttagagtca gatttcagaa aaatggtcta aggcataaag ttttttatat
     6121 atttgcctga taaatgaaaa tcggcagtta tatatttgaa cttctgcatc agcatgattg
     6181 tgtgatagtt ccacaattcg gaggctttgt tgcgcgttat aaaaatgcaa cgattcatcc
     6241 ggttcaacat ttattttctc ctccatcaaa aatagttgca ttcaacagcg aattaactac
     6301 aaacgatggt cttctggcaa attatatcag tcagaaatta ttgctgtcgt acagtgattc
     6361 ctgtaagctg attaatgatt ttgtgacttt atgtaaagcg cagcttgaag ccaaccaacg
     6421 tattcaaatt gaaaaaatcg ggtctttgtt ttttgatccg gaaaaaaact tgcagtttat
     6481 cccggctgac aatgaaaact ttttgaccga ttcatttgga ttaagtactg ttcagtcacc
     6541 tgccattcag cgcgacaggc catttgaaat tttcagcaat agagatgcag cagttcaacc
     6601 ggcaccaaag cgaacaaatc actctaagtt gattttaaaa attgctgcat acacaggagt
     6661 ggctgcatta attgcatttg cctattttaa tccggctatc aactcaaaaa taagtaaagg
     6721 tttagcaaca attttccctg ttacaactga aattgactca caagcaaatc attcttctgt
     6781 taacaacaac caagagcaaa aatccaaaga ggatttaatt tattctgaat cacctgtaag
     6841 cgaaaaaaac atttacgaaa ccgaaaaacc aacagtagat tcaagtgcaa gtcagataac
     6901 tgctcctaca cctgaaaaag aaattgctgc tgttgaagaa gaacctaagc ctgaacctat
     6961 tgtagaaaca aaaaaaatag ctgaaaccgt acctgcagtt tcaaatgcta caagacaata
     7021 tcatattgtt ggcggatgtt tttcagttta tgataatgca ttgaatttag atgctgaact
     7081 taaatcaaaa gggttttcgt ctgcgctaat tggcaagaat aaaaatggtt tggagatagt
     7141 tgctattgca tctgttgcaa ctaaaagtga agctgaggaa gctttgcaac aggcacagaa
     7201 ttcaggttac aaagatgcct ggattatgcg gaaataaatt agtggaataa agttctttta
     7261 tttaagaaca aatctggctt gtgtttaagt tttaatccca acaactttac gagacaaaag
     7321 ttgatgtttt ggctgcagcc ataaaattat cattaattta ccaaccaaga attgtgattt
     7381 ttataataca taagtaaatt aaggttgcca tcaattcttt tattctgaaa ccgaatccta
     7441 ttagacacag gaaacacgct gaaaatcttt ttgaaaaagg ctaaaattag cataaatacg
     7501 ataatcaaca gaaaggcaaa tgctcgaact aaagaaaata aatatctcat gggaacaatt
     7561 cttaattcaa taaaaccaaa aaatatacca gataattata ttaaaggatt aacattctca
     7621 ttagcaaata ccattgcccc ggcaaaccgg ggctgatggc ctctttatat agacttattt
     7681 ttaggtcggc tttcataatt cagttttagt aacccggctg ataaaacccg gggttattat
     7741 ctggcgattt attcaattct ctactttcaa aaggttgata caatattata cctgatgata
     7801 attatctaca aaacttttag gatgaaatgc aaataatgca tcaaattttg tctattttca
     7861 ggatgaaatg cactatagac acaaaccacc ttgcttatat gccttaaaat gccaaaaact
     7921 gtttcaaatg ctgctgcagt gccatttttg ttttttcgtt cttgaactca tgtgcttcat
     7981 ctatttcgag atgtactgaa gaaacaggca aaatatgagg cattacagtg atttttgtat
     8041 gttgcaaaat agaagtaaga tgatcaattc cccttaggtt acctgcacgg cctgtagcta
     8101 cacctactac tgctgctttt ttgaagtaaa gatatttctt gacatccata gcatcaatta
     8161 atagcttgag aatacccgga aaacttccat tatactcagg cacaacaaaa agtaatttat
     8221 ctgaatttaa aaaatattta tcacctatct gattaatctt actgtcgttc ttatcataca
     8281 tatcttcatt aatgatgtat tgattgagca atgtaagtga caggtaatta acttcgactt
     8341 tagcatccaa aaatgcctga taataaattc gggctaattt ctcagtgtga ctgttcttat
     8401 tatttgttcc tgcaattagg gttatcattt tgcttaatta ttcaagtgta acgattccaa
     8461 gcaatttaga aaacgcataa atatactctt cgtaaatttt ctgctgtgat aatatttcaa
     8521 ctaatctttc atcacttaaa gatgcttctt ctttcaggag caaactgttt tttcttttta
     8581 gtatctgcac acgttttaac ttgatatgat ttatcgaatt cacaatggcc tcgttaaaat
     8641 tattttcttc cgtcttaaca tatatacctt tcgattccca atcacttaat tcgtatttat
     8701 tcatcagtaa ttcgtgtgcc tttgaacgaa gtttttccaa ttcactgcta ttttcagtct
     8761 tcagcataga taaaaaatct gttgtactca tagaagtcaa atgactgaaa acacctgcaa
     8821 atatttcgtc atcaaaacca aaaccatcag gtaaaatttg ttctattata aactcacgaa
     8881 ccgtagttgg cttttcgcta ccctgaaaat taaatttctt atctccataa ttcaacagta
     8941 agcgaataat ttcctgttcc tgataccagg catttgtttc agttaaaatg gtttgctgcg
     9001 gcaggtactc catttcaatt aacttctcaa ccaattctgc ctgctgatgt tcattatctt
     9061 tcttgtataa gttcctcctg attttattca gctcagtaat tagaacttgt tccggaacat
     9121 ccatctgtgc tgcacactgg ctggtataaa gacttctttt aataccatca ggaattcgtg
     9181 aaatgctttc tacaatatcg cggatgagtt ctgccttacg aacaggatca ttagcaactt
     9241 cttttaaaag cagaccagtt ttaaatccaa taaaatcaac agcatttttg ctgatatatt
     9301 ccatcaattg tgtggtgctg ttttttcggg caaatgaatc aggatcatct ccatccggaa
     9361 ataaaacaac ttttacatta agcccttctt caagaataag gtcaatacca cgcaaggatg
     9421 ctttaattcc tgcagggtca ccatcataca aaattgttat gttgccggta aacctggcaa
     9481 taagtcttat ttgttcagga gttaatgaag tacctgaaga agcaacaaca ttttcaacgc
     9541 ccgcctgaaa taaagaaacc acatctgtat atccctctac cagaaagcac tcatcatgtt
     9601 gaataattgc tttacgagca aatgaaattc cataaagcga tttgctcttg tgataaatat
     9661 ctgtctcggg tgaattaatg tacttaggac ttttgggatc atttaccaat tgcctagcac
     9721 caaatgcaat tacacgacct gaaagattgt gaattgggaa aattaccctt ccccgaaaac
     9781 gatcataaac tttctgacca tcctgttcct tctctttgac aactgcaagt ccactcttct
     9841 catacaattg tctgttgaac ttattctcta taacagcctg cgacaaagca tcccattgat
     9901 caggagcata acccaactga aatttttcaa ttactgcttc attaaagcca cgttcattaa
     9961 aataggaaag gcctattgct ctcccctctt ctgtattgaa tagtttttct gaaaaaaatt
    10021 tctgtgcgaa agaatacaag acaaataaac tttctctttc atcttgtttt tgaatttctt
    10081 cagcagtagg gattgtttct tctatagtaa ttttatactt ctctgccaga taacgtaaag
    10141 cttcgggata agtaaaagcc tcatgctcca tcataaaccg caccgaatca cctgcttttc
    10201 cacaaccaaa gcatttgtat attccctttg ccggtgacac cgtgaaagat ggtgttttct
    10261 cattatggaa aggacataat ccaagtaagt tagcaccacg tttttttaat gaaacaaaat
    10321 cgcctaccac ctcctcaatt ctggaagtct caataatcaa ctcaacggtt tcctttggta
    10381 tcataatgcg gcaattcttt ttctaacttt tattttgggc ttttcggatg aaatatttga
    10441 gttaataaat gctgccattg ctctatacat attcttctct gcatctgtat ctatcctgat
    10501 gaaacttctt gtttgattgg tccaaatttt tttgttcggt gattttgctt cctcaaaaat
    10561 acgaaccgac tgaataattt ctaaatctgt agcactatca gaaatagcca ctaaaagcgg
    10621 agttcttaaa tgtcctaaat actcttgcaa atttaatgaa tctgcatcta taccggtctt
    10681 ctgacggtac ttaatttgtg caaatggagt tactaacatc gaaaaaccat tccacttttc
    10741 tttaaccaac tcattgattt tagctatcat gcccggtaca ggatctacaa gaatcatgca
    10801 atgactgcga ctatccactt taatccatga tgctgctata atgcctgaca acccataagc
    10861 aaccactgca atgttgtctg tttcgtacaa acaatacaaa ctgtcagcta ccacttccag
    10921 acaatggttc attgattgat ctatgataaa tttttcacca tcgctctcac catgtgctgg
    10981 taaatcaact acgcatacat taaatccaag ctgatacaag gcttccgctg tttttatcat
    11041 actcaacttg ctttcattcc aatcatgcaa caacagtaca gttgtggttg caggctgttc
    11101 gcctgaaata taccaaccat gtaatgtaaa atgatcatga atcgccacgt caaaattccg
    11161 atagttaaga ttcagctgtg aaggagaaac tattacatct ttatctataa ccaattcatc
    11221 ctttttcaga ttgtgaaatt cagcagttaa tgatttaacc gttatcctgt ttgggtaaat
    11281 taattcgctg atgctgaata atgatgtgaa atatagcagt aaaaataaac tgccggcaca
    11341 aattaaactt acaacaatca ctttccttaa catcacctag taattgcgat ctgtagtaaa
    11401 cgtaatcaga tcatgcaacg acttacggta atcactatca ggaaaactat tgataatttc
    11461 aaaagcatga ctcctgagtt caaacatttt ttctcttgca taatcaatac ctttttgcct
    11521 attgacgaat gccaaaactt cagcaacctt actttcattc tcatgttcat ttttaatcag
    11581 gttgatgatt gttctttttt ctgaggaagt agcttgtgac aatgcataaa taatcggcag
    11641 tgtaagtttt ctttcacgaa tatcaatgcc tgaaggttta cctgtagaga tacttttttc
    11701 aaaatcaaaa aggtcgtctt taatctgaaa agctaaccct attgtctctc ccaattcgcg
    11761 cataagttca accttttcat cacctgcctg tacagatgct gcaccacaac tacaacaaga
    11821 agcaattaat gatgctgttt tctgacgaat aatcttatag taaacttcct catcaatatc
    11881 gagtcttctt gccttttcca tttgcagcag ttcaccttca ctcatatcac gcactgcatt
    11941 actcacaatt tccaaaagtt tgaactcctt attctgaaca gcaataagta acccacgtga
    12001 caataaataa tcgcctacca gcactgcaat tttatttttc cagactgcgt taatagaaaa
    12061 aaaacctctg cgctgtaaag catcatcaac aacatcgtca tgcaccaaag ttgcagtgtg
    12121 caataattca ataagagatg ctgctgtgta tgttgattca tttatttttc cgcatacacc
    12181 ggctgaaaga aaaacaaaca tgggtcgcat ttgcttgcct ttgcgcttaa caatgtagtg
    12241 catgattttg tcaaccaacg acactttgct gcgcatggca tcgcgaaact tgttttcaaa
    12301 gcagtccatt tcgagcgaaa tgggcttttt aatctgatct aaactgagcg acataataaa
    12361 cggctgcaaa ggtatgtttt taagtagcag cagcctttgg cagtttactg attcttatta
    12421 gctaattgac cacatgcagc atcaatatct ttacccctgc tgcgtctgat attgactact
    12481 aaattcttgc tttctaaata atttttaaaa acctctagtc tgtccaatcg agccttttca
    12541 aaaacaccat cacctattgc attgtattct attaagttaa ttttacaagg aacatgtttt
    12601 gcaaattcag ctaattcttg ggcatcttta agtgtgtcgt taaaattgtt aaaaacaata
    12661 aactcatagg ttacacgtgt gcctgtttta tcataaaaat acttcagggc atcacgtaaa
    12721 acctcaagat tattatgttc attgatgggc ataattttat cacgcttact gtcattggca
    12781 gcatgtaatg atagcgcaag attaaacttt acttcatcat caccaagttt tgtaatcatc
    12841 tttgcaattc cggctgttga aactgtaatt cgttgtggcg acataccaag tccgttagca
    12901 gatgtaattc tttctatgga tgcaagcaca ttggcataat ttaaaagagg ctcacccatt
    12961 cccatataaa ctatgttgct taatggaata ttatagacct ccatactttg ttctctgata
    13021 atagccacct gatcataaat ctcatcagca ttcagattgc gtacccgatc taatttgcct
    13081 gttgcacaaa atttgcatga cagactgcaa ccaacctgag aggaaacaca agcagtcatt
    13141 cgtgacgcag tggggattaa aacaccctcg attaaatgat cgtcatgcag tttaaatcca
    13201 tacttaattg ttctgtcgtt acttcgttgt tcatgtgcaa caacaatagg agtaaatgca
    13261 aacttctctg ccagtttatt tcggagtgac aacggcacat tgttcatttg gtcaaatgaa
    13321 cgtgcacttt tattccagag ccagtcgtaa atctgtttag ctctgaatga cttttcgcca
    13381 aaatcagcaa cggcttcaga aagttgttcg aaagtaagct cccttatgtt aggtattgat
    13441 ttcactttgc aaaggtacga tgaaaacatc agccgataca caaatcactt agttttgtaa
    13501 gcatgaaatt ttattcggca gatattgttt tccctatttg cagtactccg cttaaggatg
    13561 gtatagttgt aatggacaat tcaggagtag tcattgatgt ttttgaaaac attgcatttc
    13621 tgaataatgc tttacaaatt cagatagagc catcagtcat tgaaaaattt catggcatac
    13681 ttacaccggg attcattaat tcacattgtc atcttgaact ttcacacctt aaagggcgaa
    13741 taagtaaaca taacaagctt acaggcttta tcaaagaatt gatttcggtt agaaagcagg
    13801 gtgagaatga agaagtatta gacgctataa aatcagcaga gaaaaatatg tttaatgatg
    13861 gcattgttgc tattggagac atcagcaata cttccataac atttccaata aaaaatgaat
    13921 ctgacttact ttaccatact tttattgaag tatttgacct atcagaattg aatacagaaa
    13981 aaacatggaa cacagcaaag gaacttgaag gtatgctgaa atcgaataac agaaattcaa
    14041 aatgcagtat aagcccacat tcaccttata gtgttacacc tttcttgttc caaaaaataa
    14101 acaaaaacaa agcgtcttta atatctatac ataatcagga aaccgaatcc gaaaacatct
    14161 tgtttgaaac aggctcaggt gatttaaaat ctttctttga agcaacagga aataatttag
    14221 tacatattaa agccaacgga aaaaattcaa tacagaacta tgtaccacta ctgaacaatt
    14281 gcgatgaaat catttttgtc cataatacat tcagtagtga tgatgatata cagtttgcta
    14341 ttaaaaatgt aaagaaagca tggtggtgtt tttgtccgaa tgccaattta tatattgaaa
    14401 atacacttcc tgaaattgag cgaataacca aagctactga gaacatcctg attggcactg
    14461 atagccttgc atcaaacgat caattgagca ttttatctga attaaaaatc atcacaaata
    14521 aatttcctca gattacgcta catcaacttt taacctggtc aacaattaat ggtgctaagg
    14581 cacttaaaat ggataatgaa cttggcactt tagaaaaagg aaaaaagccc ggcttggttt
    14641 taattgaaaa tgttgattta caaaatcaga aaattacgga aagcagtaag tcgaaaaagg
    14701 tggcttaacc taccaatcgt ctttcttagg cttaggcgga tttgacatat atttctctaa
    14761 ttcagcaatg attttatgcg attgctcatc ggtatatttc aaattccaat cccagtcttt
    14821 ggtgtaatat tgatttttag tatccatcca acgttcaaca ggaaaataag aaggttgctt
    14881 ccagttaatt tctgtcagct tatatctgta tttaccatct ttagcttcaa ttattaatga
    14941 atactgaaca ttaccctgat ctttctgaaa gccactttta tcaggctcgc ccatcaattt
    15001 aaatcttgct ttgcaaagta attttccatt ggctttatca ttttctcgca acacgtcagc
    15061 agggttttta taataagaat taatccaccc taatgctcta tcaaaaatat cattcttaga
    15121 cactccggtt aactctttta ctttggtgta gcaaataagt tttgaatcat tgtcaacagg
    15181 ataactgaca gcctgtgcat ttatctgact aacactaaat gctactatta aacttgtaat
    15241 gataagtttg attttcattc tgatttggtt tgattaaaaa atcagctatt cactgacact
    15301 tttattttac tacctgcttt gaggtctttt gaattcgaaa ttttatttat tcttttcagt
    15361 tgatcaacag aagttccggg atattgttgt gcaattttcc ataaggtatc accgggctgt
    15421 acttgatgat atataacctt caatgcaggt ttattttgtg atgttggcag ttgttctgtt
    15481 ttaggagttt cctgcttgcc ggcaacattt tgtggagcgc aatcttctgc attgtcttcg
    15541 caatcatcat aattatcaat aacctttgtg ctgacaggaa tttgtttctt tacatgcacc
    15601 cttacataaa gtctttgatt tttaagaatc cggcttgact ttagtccatt ccatttttta
    15661 agttcattaa cagaacaatt atatttatca gctatgtcag ataaagtttg gcctgatttt
    15721 acaatgtgtg cttttttctg aattgaagtc actgttttat aaactatttc ctgattcatc
    15781 acaggcacat tactgcttgc aatttcgttt acagtttttc tgtctgcttt agcaaacaat
    15841 ggatcaactt tttgatttcc cataggccca aagcccaatt gtgtgtaata attattgagc
    15901 atttcgccaa caatctgatt gcgcaaccaa gggtttgatg cacctaatgc aatggcaatt
    15961 aagcgatgat cgtttttctt tgcagtagca accaaacaga attttgcatt ttttgtatat
    16021 cctgttttta gtccgtccac ttcaccggga tagtccataa ctaaatgatt atggtttctg
    16081 taaacattat atccatgccc gttttcaact tctgcaaaag caatattggt aatggaaaga
    16141 agttcgggat atttgattaa ttcagttgac agctttagca aatcgtaagg tgaagagctg
    16201 ttgtcaaggc ttccatagcc tgcaggtaat cctgatggat ttgaatagaa agtttgtgtc
    16261 attcctaatg ctgctgcttt ctgattcatt cttgtcacga aagtttcaac agagccactg
    16321 atatgttttc ctattagtcc gcaagcttcg ttatttgaag gaatcatagc catctttaat
    16381 aatgactcaa gactataagt ttcgtttacg gtataagtac gcttgcttct tttacttttt
    16441 ctgtatgtct ttgtcacaga aacctgatct tgccaatcaa cagtaccaca tttaatctct
    16501 tcaatggcaa taagggcaac catcattttt gttagtgaag ctataggata ggcatacttt
    16561 aaatcttttt cccatacaat ggtatttgtt gtacaatcat aaatgagtcc tgcacgaatt
    16621 tgcgaagtgc tgttatcact gagtaaagca ggttgtagcg caatttcaaa atctttaggc
    16681 ttctctgcaa catggacatt ttcatcaata ttagcagaaa gtgtatcgtt gcctccagcc
    16741 caaacattgg acacaaacaa catcagaaat accaatggca ttataaacat agcatttatg
    16801 agtttttttg aaaacaatgg aaaagtgttt tgcaagttta tcatttaatg ctttgaattt
    16861 tttattgctc caacaagtga tttacacaac ccgatgttga tagtacttaa acgtaaaaaa
    16921 ctttgctatc gtttctttca acatcaaata tttttttcaa tgtaacga
//
LOCUS       DAOOOI010000047        16323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_713_length_16323_cov_84.398574, whole genome shotgun sequence.
ACCESSION   DAOOOI010000047 DAOOOI010000000
VERSION     DAOOOI010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 16323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16323
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_713_length_16323_cov_84.398574"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(425..1303)
                     /locus_tag="PKX59_07085"
     CDS             complement(425..1303)
                     /locus_tag="PKX59_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90668.1"
                     /translation="MNNGLYNGLDSGLNQGRFSGLDNGLALGLFGNVRAKLDKDVIPY
                     IENAKIMRAQEIFAINYLVTNTKKFFLWDKIKAIYPFLGDTLLSNAFNLKIPIDSNSA
                     YRLNFDTNLPLITRFGVEFIGNSSSWATTNLIPSQVLIKGNLALGCYINGGNLGGSPY
                     PVTMGSSTTLNTNTDVISLAIRTTGKYFIPNGEAQPFVSSSDTTITGNYIGTALNGLA
                     NLYRNGIELGNTATSTNGGISGSPIFIGALGQNISSTYQNVRLGICFISTGLTKIDVR
                     NFDMIIQNYVSILNRR"
     gene            complement(1303..1758)
                     /locus_tag="PKX59_07090"
     CDS             complement(1303..1758)
                     /locus_tag="PKX59_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90669.1"
                     /translation="MALAAKNIPVVANVPNGGVALIAASSNTVTLGSNTNGATAYTAG
                     TAGGRVMSLTAVTDDTVTVNVFVWILRGATVIPIALVNVPLSSGNTNAARFNVDFLDG
                     VNIVGLPLDNTGKRYIPLLAGDVLRVGALANLTAAKTCWVHASGSDYQA"
     gene            complement(1742..2440)
                     /locus_tag="PKX59_07095"
     CDS             complement(1742..2440)
                     /locus_tag="PKX59_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD3 domain-containing DNA-binding protein"
                     /protein_id="HOZ90670.1"
                     /translation="MALVGIYKITSPSNKVYIGQSVNILNRKASYKRQDCKKQAKLYM
                     SLLKYGFDKHSFDILCLCDKSELNEKEKSYILEYDSFLNGLNLTSGGESCNVRSDETR
                     MKMSLAQKRNTKSKGHKHTEESKKLMSENRKGKVISETGRKNMSLVQLGKKMSDETKQ
                     KISDKLKGKKKSKEQILKFSKSMIGNTNRLGKKKTLEERLKISKSKMGSIIPLEVRNK
                     ISLTLKNKYHGISS"
     gene            complement(2662..3270)
                     /locus_tag="PKX59_07100"
     CDS             complement(2662..3270)
                     /locus_tag="PKX59_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90671.1"
                     /translation="MSIAALKASIASVIKLNGSKSIYATHLQAKLFEMIDEMHPENYI
                     SGNETASTLGATINGASAAIPNNTDLVATAENAGLLKKITWTNVKAFLKTYFDTIYQT
                     VLISGTSIKTINSTSLLGAGDISITPNATHTGEVTGTTALTVDKTAITNKTAVTIDTA
                     DYVLISDSSDSGNLKKGLVSDLGGFDDGDFTLVASFKSLYNY"
     gene            complement(3281..3925)
                     /locus_tag="PKX59_07105"
     CDS             complement(3281..3925)
                     /locus_tag="PKX59_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90672.1"
                     /translation="MTFVLKNIIFDFFGIRDKSEDLYKDVNGKGLHQRFNEMLAEDVD
                     ENEIDKTENVVKYTVDPDQCLDKFFFYREQEYGGLYSISAVPAIRRKIIKYASYLNRI
                     KGTEESYIFPLLLLGFSTVNIYEYVNTSGFDSPTTFDDEVRRFDMKCNSCGRYRIDIT
                     GSITMTNSLLNSIMNVVRYNEPINATLDGITYNGDTISIVGDFSDDFSDDFFND"
     gene            complement(3925..5592)
                     /locus_tag="PKX59_07110"
     CDS             complement(3925..5592)
                     /locus_tag="PKX59_07110"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HOZ90673.1"
                     /translation="MALTNPFIGYLDRSFLTIKQSILTRIANPVTGIPEITDHSDSNP
                     FVKRVSIWAGIAEMLGYYIDNMAKETFVHTADLYESFYRLSKAYDYRIRGRIPASGTI
                     NITLNNPAPYNITIPSETEVQTSEGIKFLTLSSAVIVTGQTNVSIPVKQWTKVVASVI
                     AISDGSQNQVYELNENVVDKSINIIVDNTIPYINVDSFVRSLPLSYHFIAGMNENAKM
                     QIVFGDDINGAIPLATKDIKVGWYETSGRNGNVGSGLINTINSVLTLPTGFTASVTNL
                     LAITNGTNAESITELRKYIPLSLRTLYRAVTRQDYIDVAELIPGVAKAGLEHSCGKTV
                     DIYIAPNGGGAPSPTLLTDAYDFMELRKMITTKHRIFGAGQVIIKHLINVRSKPQYFN
                     VDTYNEVKEAVENFYNVENQTISGKVHIGDIYQLIEGLPKVEYSTVGILTPVPYARPL
                     DTGYPILDWAKEVLISSQNATYKLVFTTSTQFNLVRNNVFVNSYSVGVLVTTNDLKFT
                     VFGTYTIGNKYEFKTYNYNSGSVDLAEPSLPVFDITEFELNVTGGLV"
     gene            complement(5595..5981)
                     /locus_tag="PKX59_07115"
     CDS             complement(5595..5981)
                     /locus_tag="PKX59_07115"
                     /inference="COORDINATES: protein motif:HMM:NF016829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HOZ90674.1"
                     /translation="MNLEYLGVSEKFTFQPDLNSRVALVSDVESIKQSMKDILSTGIG
                     TRFYNEYYGSYLEYLTFEQNDVILKDLLIYYSIDALRTWEKRIKVLNVDVKVGVDKME
                     LLIPFLILQSNEIQSLIYPFYREITQ"
     gene            complement(6003..6710)
                     /locus_tag="PKX59_07120"
     CDS             complement(6003..6710)
                     /locus_tag="PKX59_07120"
                     /inference="COORDINATES: protein motif:HMM:NF016595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HOZ90675.1"
                     /translation="MSAFREFLDNMTSGGLEYFGLYYGKYEALVVDCDDPEKRGRLKI
                     SCPRIYGNEKYDKWVYPSGLFAGKNYGMHFIPQNGDVIRLTFDGGNVNFPVWEYGWWL
                     KSKSIQIADKDIYCIVTPKGHSWVIDEKNDRIYFSYKDGKVIEIKGQKISLGKNGGSA
                     EKAALGETLKKRLEEICDNQKDICDAMVAMTVTCNPPGSPSTTPINSALFAQYGARAT
                     TIKSQLIEILSEVVTLD"
     gene            complement(6710..7939)
                     /locus_tag="PKX59_07125"
     CDS             complement(6710..7939)
                     /locus_tag="PKX59_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90676.1"
                     /translation="MASSPYYIIQIKESGRNITNYVTNLHYEDCIKEDDLVELKIDGA
                     TLELIDSDDFIVGNHVIYSFGLGERVQSGQRLAVIKDIDVDYAKTISMTIKCRDTGFY
                     MKKETSNKVYTNKTSSQIVSDICNLYGYVPNIDESTFVHKSISQGNKTFHQFCNELAK
                     KEGSNGKDGSFEFYVRGNELYFQKRNLKKDSKRLFTYGDGNDIVKYFNPSYSQENEGK
                     SDSVTVAGIDKETGEQFTSKAGSKSNPETALGKVQHSFDWKGKEVGISNPAPIGEVIT
                     TGKNIAVPVDDKVAADKITSASQKDANKSQLQMEMGIDLDPTINAGDIITVSGVAKKH
                     SGNWYVTKVVHQIGSGASTVISADKNASNKSLTNNTAKTSTNTSQNNDVNETKGKNSQ
                     EQVKKIHSYNWEGKEKK"
     gene            complement(7939..8256)
                     /locus_tag="PKX59_07130"
     CDS             complement(7939..8256)
                     /locus_tag="PKX59_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOZ90677.1"
                     /translation="MAGLYLHDFDLYSNGRIIVLPSGNKLLKRDKIKYSPQEPDIYHT
                     VVAGDTLTFLAWKFYSRFTSDAPKYWKYIADVNNIKNPLDIRQYIGKNIVIPNFALIK
                     LSE"
     gene            complement(8257..8667)
                     /locus_tag="PKX59_07135"
     CDS             complement(8257..8667)
                     /locus_tag="PKX59_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90678.1"
                     /translation="MIQFVPENISNPRKANLQDVVIVGRNNEMMQYVSGSENMTLNLD
                     FYSDDVERKDVIKTINWLKSLTYNDGAPGSYRNVRVIFGELFKNDVWVIDSVDPDMSI
                     FDAENGWLPVMAKVKVSFKLDTQRNINLNDVRDR"
     gene            complement(8739..11237)
                     /locus_tag="PKX59_07140"
     CDS             complement(8739..11237)
                     /locus_tag="PKX59_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HOZ90679.1"
                     /translation="MKKRKWIKQNTKSKSMIFGGGSVGGGAIGTGIVFTLYDQFSATS
                     DKIATKFKTLEGISEQAANKIDSSLSKMKLGFASMVVGAGILATLAFPIDKAAEFEQG
                     LSAIKAVSGATNAEMKILSDLSLEMGKKTKYSALESAQGIEELIKAGVSLKDVVNGGI
                     EGALNLAAAGEIEVADAAEIASMALNAFRNDALTVTKAADILAGAANASATDISGLKM
                     GLSQASAVASGFKMTFLDTSTALAVFAQNGLKGSDGGTSLKNMLLNLIPTTKAQIELS
                     KKLGLVTADGANAFFDSTGKMKDLAGISDVLQKSFSGLTDEQRASAMVTLFGTDAIRA
                     SNILYKEGAKGVNDMQQAMTKFTALSVATERMNNLKGATEQMKGSFETMMIVIGSGFT
                     KPLSLLVKGFQFLFDIVTSLASTKLGKVLLITFGIIGAGALVLGAFVLVTNLATFASG
                     QLALAFASLGKTAIATAFAEQGLIAGTKALTASLLPLIWEFLPIIVAVASVIYLFYRM
                     KKGFEDFNAMADGTGKKLSGISGVFQKIGGVIQAFMEIWSSATDDGFTFSDNTYKALK
                     NMGIYEFAVNMGVAFGYIRQGFQMLGKVASAIWNVLQPIFGSIGNLIGTVAIIIGVVL
                     VGAVIAWTASMIGAAIATLAATWPILLIIGIIIALVYVIQNFGKIWDWVVEKFKAGWH
                     SMVEAGKSIFMEFIVWLGGLGIQFFNWGVSLVQSIGDGIISAWEWLKGEMLSLIADLP
                     GGKILLSALGIDVPEANGTGNGGNVSGPTPLTTPSAIGTTIAQQKSVRGENTNSFTQT
                     VEKKETLQTVKLQIDGRELKTALDKVDKEQNSRK"
     gene            complement(11197..11370)
                     /locus_tag="PKX59_07145"
     CDS             complement(11197..11370)
                     /locus_tag="PKX59_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90680.1"
                     /translation="MGFRVMDAWFDLSYGGLSISLEELYAMPYDNIYSLLMRMDIQKK
                     HEKAEMDKAKHKK"
     gene            complement(11382..12134)
                     /locus_tag="PKX59_07150"
     CDS             complement(11382..12134)
                     /locus_tag="PKX59_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90681.1"
                     /translation="MLLECVTRIGSNSSPNLKDIQNLLSEDRKEMLMALRQLSNGFNP
                     TFTFKYEFPTQNSKRLEQTYEVIFDKEDFPKKPYEWTLKAMEISYKEERGIPEQDELN
                     NEQKNEVMQLPFPVLFEDYNEMLKLYKKQTLKLPESGVTVVYNLLDGKTENNYGKTLK
                     SNKISSHTYIVMRDAKYLDPDKLEQGKEIPIILPLGELSIVDIEFLRGEMRKVEADIH
                     TTVVVQYKGDASIQAQVNLIATPAFFFPSLAL"
     gene            complement(12429..12692)
                     /locus_tag="PKX59_07155"
     CDS             complement(12429..12692)
                     /locus_tag="PKX59_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90682.1"
                     /translation="MEKSKKNSHMMNAETWRAYMYMKVTLENIYSGKYPNGVKFKPKD
                     YEDAKEQVELCTQVLMDHNVDLPIYEAPVFEPVAGSQLDLFMV"
     gene            complement(12698..13081)
                     /locus_tag="PKX59_07160"
     CDS             complement(12698..13081)
                     /locus_tag="PKX59_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90683.1"
                     /translation="MKAVLSQQNGSSMHSYHNTTGMQKPELKAAEQKAQKQDVLVLQV
                     FKDNRLKDFTPCEVYEYMIKKGVISRKVPITSIRRAISNLTATGKEDLEKLPGKREGA
                     YGQPNRTWRLNRVKYNVAEQMEMFN"
     gene            complement(13093..13563)
                     /locus_tag="PKX59_07165"
     CDS             complement(13093..13563)
                     /locus_tag="PKX59_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90684.1"
                     /translation="MYGLKATTKSNYCLVMVWQKDSLDNNRHILLHLAPELRAKLQVA
                     FSIIDGAPVFNPNIDKSNLQFTDIGFDITKHVKLIGNLIDGSTDAIDEMFTESDKILF
                     VEKVDTSEFAEYFTEVRQAILHLKDELFSIEIIGVNEEHDLWLADAIETKFIKH"
     gene            complement(13663..13821)
                     /locus_tag="PKX59_07170"
     CDS             complement(13663..13821)
                     /locus_tag="PKX59_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90685.1"
                     /translation="MKIKDFIKVFGESGHSARFEGCTAKNGDKATGEYKVEANEVLYT
                     YTDKKERT"
     gene            complement(14114..14302)
                     /locus_tag="PKX59_07175"
     CDS             complement(14114..14302)
                     /locus_tag="PKX59_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90686.1"
                     /translation="MKKELKQAIINFIFENEKDFQLNNRTSNEFSAYMYDSSGNYLIG
                     GEDVARFIRDAIKLLTEQ"
     gene            complement(14313..14474)
                     /locus_tag="PKX59_07180"
     CDS             complement(14313..14474)
                     /locus_tag="PKX59_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90687.1"
                     /translation="MQNTAEKYKIVLLTTQGLKTEIYSTSTPLEDFTAEMVKKYGTFT
                     TYTSNLITN"
     gene            complement(14480..14833)
                     /locus_tag="PKX59_07185"
     CDS             complement(14480..14833)
                     /locus_tag="PKX59_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90688.1"
                     /translation="MLSHTIKKTAKKLFSGKYKGLFMLLEINQHAISFKNVNGVVFSY
                     QSGPDIENFFNLFPGCGFLSAGYVKEGTVYGPTFKSEYNTCGLIDTAEYTAFYQQATD
                     NPDFIKRTALQITLN"
     gene            complement(14870..15169)
                     /locus_tag="PKX59_07190"
     CDS             complement(14870..15169)
                     /locus_tag="PKX59_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90689.1"
                     /translation="MNNYNSIKAKIQNEGCYIEAETYKNGVTIHSLIKREDETTKTIF
                     CEIAANIAKMLEKETGKAITHKEGLTVALNFNPVPVKHNMVRTFHYNDTEEKLFN"
     gene            complement(15234..15527)
                     /locus_tag="PKX59_07195"
     CDS             complement(15234..15527)
                     /locus_tag="PKX59_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90690.1"
                     /translation="MNTATATTAQEINLISYNIHGQKLREGIIKIDFEKEKVIFPSGK
                     EYSVINDYEFTYKILKGTIKAAGGINGLSKFFNEINRQDSPFNPCYTNLFIEL"
     gene            complement(15538..15762)
                     /locus_tag="PKX59_07200"
     CDS             complement(15538..15762)
                     /locus_tag="PKX59_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90691.1"
                     /translation="MQTPPSQEVQTAINENNKVLDFIKTWQSKNEYNPKFLDEFNAKF
                     NAEFNDFLKAGNFTAETIKFFIYKPDLITK"
BASE COUNT         4587 a         3326 c         2434 g         5976 t
ORIGIN      
        1 cctgtgtatt cgtacttatt tttactgctt aaatgtttgt gccaatattc gtatggagtt
       61 ttatttaact tggactcctc acttaggtcg catatcattt ttgcatagcc ggaaatacag
      121 ccaagtgcgt gagcaaacac ggaaaataca actgataata tgattaacgt tgttgtcata
      181 agttcataaa ttttttggtt gtgttataaa tagttatatt ccgaaccttc ctcttaaatt
      241 attaaagtta ttcataactt cttctttact caaagctttt ttataaaact ttaatactgc
      301 aacatcagcg ttagtatggt aagaatctaa ataactatta tgtattctaa tcttttttcc
      361 tgaatgattt ataatagaat tttcattttt gatattgttt agtttttttt aaatattaaa
      421 tagtttatct tctatttaat atactaacat agttttgtat aatcatatca aaattacgta
      481 catctatttt tgttaatccc gttgatatga agcaaatacc taatctgaca ttttgataag
      541 tactacttat attctgccct aatgcaccaa taaaaatggg tgaaccagat attccaccat
      601 ttgtacttgt tgctgtattt cctaattcaa taccatttct atatagattt gctaaaccat
      661 tcaaagctgt gcctatatag ttacctgtta tagttgtatc agaagaagat acaaaaggct
      721 gtgcttcacc atttggtata aaatactttc ctgttgttct tattgctaaa ctaattacat
      781 ctgtatttgt gtttaaggta gttgatgaac ccatagttac aggatatggt gaaccaccta
      841 aattgcctcc gtttatataa caacctaatg cgagatttcc ttttatcaaa acttgtgaag
      901 gtattaaatt ggtagtagcc caagaagaac tattaccaat aaactctacg ccaaatcttg
      961 taattaaagg aagattagta tcaaaattta atctataagc agaattggaa tcaataggta
     1021 tttttaaatt aaaagcattt gataacaaag tatcacccaa aaacggataa atagctttga
     1081 ttttatccca taaaaaaaac ttttttgtat tagtaaccaa ataatttatt gcaaaaattt
     1141 cttgtgctct cattatttta gcattctcta tataaggaat tacatcttta tctaatttag
     1201 ctcgtacatt accaaacaac cctaaagcta aaccattatc taatccggaa aatcttcctt
     1261 gatttaatcc gctatctaaa ccgttatata atccgttatt cattacgctt gataatcact
     1321 acctgatgca tgtacccaac aagtttttgc tgccgttaaa ttcgctaatg caccaactct
     1381 taaaacatca cctgctaata aaggaatata tcttttacct gtattgtcaa gcggtaatcc
     1441 tacgatattc acaccgtcta aaaagtccac attgaaacgt gctgcattcg tattacctga
     1501 actcaaagga acgtttacaa gtgcaatcgg tataacagtt gcacctctaa gaatccatac
     1561 aaatacgtta accgttaccg tatcatcagt aactgccgtt aagctcatta cacgaccacc
     1621 tgctgtgcct gctgtgtatg ccgttgcgcc gtttgtatta ctacctaatg ttaccgtgtt
     1681 tgaagatgct gctataagtg ctacaccgcc gttaggcaca ttcgccacaa cagggatatt
     1741 cttagctgct aatgccatga tatttatttt ttaatgttaa tgatatttta tttcttactt
     1801 ccaatggaat tatactaccc attttgcttt tagatatttt taatctttcc tctaatgtct
     1861 tttttttgcc taatctattt gtgtttccaa tcatagattt actaaacttt aatatttgct
     1921 ctttgctttt tttctttcct tttaatttgt cgctaatttt ttgctttgtt tcatctgaca
     1981 tctttttgcc aagttgaacc aaactcatat tttttctacc agtttcgcta attacttttc
     2041 cttttctatt ttcactcatt agctttttag attcttccgt gtgtttatgt cccttacttt
     2101 tggtgttacg cttttgtgct aaactcattt tcatccttgt ttcatctgac cgcacattgc
     2161 aactttcacc accacttgtt aaattcaaac catttaaaaa gctatcatat tctaaaatgt
     2221 atgatttttc tttttcgttt aattcacttt tatcacacaa acataaaata tcaaaagagt
     2281 gtttatcaaa accatacttc aaaagtgaca tatatagttt tgcttgtttt ttacaatctt
     2341 gtcttttgta agacgctttt ctatttaata tattgacaga ttgacctata tacaccttat
     2401 tagatggtga cgttatttta tatatgccaa ctaatgccat aatattatat taaaatgtct
     2461 aaaaatgtta ttttacttag gaatcctatc gtttcaatct ttatgattaa accctttttc
     2521 caagctaatt gctgaatgtc tttctcgttc aactcatccg tgacaaagta taataagtca
     2581 cttctgttag ttgctgagtt cttccaatct atcagcttat tatcgtagtt attttgattt
     2641 atttttacgt ttggcatgat attaataatt ataaagtgat ttaaaagatg ctaccaatgt
     2701 gaaatccccg tcatcaaacc cacctaaatc agaaactaat ccttttttaa gattgccaga
     2761 atcggaacta tctgaaatta aaacataatc agccgtatca atcgttactg ccgtcttgtt
     2821 tgtaattgca gttttatcaa cagttaaagc cgttgtaccc gtaacctcgc ccgtatgtgt
     2881 tgcatttgga gttatagata tatcgcccgc accgagtaac gatgttgaat taatagtttt
     2941 tatcgaagta cccgaaatta atacagtttg atagatagta tcaaaatatg tctttaaaaa
     3001 cgcctttaca tttgtccaag taatcttttt aagaagtcct gcattttctg ccgttgctac
     3061 taagtctgta ttgtttggta ttgcagcaga agctccatta atcgttgcgc ctaaagtaga
     3121 agccgtttca ttaccgctaa tataattttc aggatgcatt tcatctatca tttcaaataa
     3181 tttggcctgt aagtgtgtag cataaataga tttactaccg tttaatttta ttacggatgc
     3241 tatacttgct tttaatgctg ctattgacat attttattta ttaatcatta aaaaaatcat
     3301 cactaaaatc atcactaaaa tcacctacaa tactaatagt atcaccatta tatgtaatac
     3361 cgtccaaagt cgcatttata ggctcgttat acctcactac attcattatg gagttaagca
     3421 atgagttcgt cattgtaata cttcctgtaa tgtcaatcct gtaccggcca caactgttac
     3481 atttcatatc gaatcttcgt acttcatcat caaaagttgt tggactatca aatcctgaag
     3541 tattcacata ttcgtaaatg ttaacggtac tgaatcctaa taaaagtaat ggaaaaatat
     3601 aactttcttc agtacctttt attctattta aataagatgc atattttata atttttctcc
     3661 ttattgcagg tacggcagat attgaataca aaccaccata ttcctgctca cgataaaaaa
     3721 agaacttatc taaacattgg tcgggatcta ctgtgtactt taccacgttc tccgtcttat
     3781 caatctcatt ttcgtcaaca tcttcagcca acatttcatt gaaacgctga tgcaatcctt
     3841 ttccatttac gtctttgtat aaatcttcag atttatcacg tattccgaaa aaatcaaaaa
     3901 ttatattttt aagtacgaat gtcattatac caacccacct gttacattta attcaaattc
     3961 tgtaatatca aataccggta aactcggctc tgctaaatcc acacttcctg aattatagtt
     4021 gtacgtctta aactcatatt tatttcctat tgtgtatgta ccgaacactg taaactttaa
     4081 atcatttgtt gttactaaaa ctcctactga atagctattt acaaaaacat tatttctaac
     4141 taaattaaat tgtgtactag tcgtaaaaac taacttgtaa gtagcatttt gtgacgatat
     4201 caaaacttct ttcgcccaat cgagaatagg ataacctgta tcgagcggcc ttgcataagg
     4261 aaccggagtt aaaataccta ctgttgaata ttcaactttt ggcaatccct caattaattg
     4321 atatatgtcg ccaatatgca cctttcctga tatcgtttga ttttctacat tatagaagtt
     4381 ttcaactgcc tctttaacct cattatacgt gtcaacattg aagtattgag gctttgacct
     4441 tacatttatc agatgcttaa taataacctg ccctgcacca aaaatcctgt gctttgtggt
     4501 tatcatttta cgcagttcca taaaatcgta tgcatctgtc agcaatgtag gacttggtgc
     4561 tcctccaccg ttcggtgcaa tgtagatgtc caccgtcttt ccgcaggagt gctccaaacc
     4621 tgctttcgct acacctggta tcaattctgc tacgtcaata taatcctgcc tcgttacggc
     4681 tctgtataag gtacgcaatg ataacggtat gtatttcctc aactcggtaa tactttctgc
     4741 attggtgccg ttcgtaatgg ctaacagatt ggttactgaa gccgtaaaac ctgtcggcaa
     4801 tgtcaatact gaatttatgg tattgataag gccggaaccg acgttaccat ttctaccact
     4861 cgtttcatac caacctactt tgatgtcttt tgttgccaac ggtattgctc cattgatatc
     4921 gtcaccaaaa actatttgca tttttgcatt ctcattcatt cctgcaatga aatggtaact
     4981 caacggtaaa ctccttacaa aactgtcaac attaatatac ggtatcgtgt tgtcaacgat
     5041 aatattaata cttttgtcaa ctacattctc attcagttca taaacctgat tttggctgcc
     5101 gtcggaaatt gctattacag atgcaacaac ttttgtccat tgcttaaccg gtatgctcac
     5161 attcgtttgg cctgtaacga ttactgcaga actcaatgtc aaaaacttta taccctctga
     5221 agtttgtact tccgtttcgc tcggtatggt aatattgtat ggtgccggat tattcagcgt
     5281 gatatttatt gtgccacttg caggaatacg gccacgaata cgataatcgt atgcctttga
     5341 tagcctgtaa aaactttcgt ataaatctgc agtatggaca aatgtttcct ttgccatatt
     5401 gtcgatgtaa taacccaaca tttcggctat tccggcccat attgatactc gctttacaaa
     5461 cggattgcta tctgaatggt cggttatctc tggtatgcct gtaaccggat ttgcaatacg
     5521 tgttaaaata gactgtttaa tcgtaaggaa cgacctgtct aaataaccta tgaacggatt
     5581 tgttaatgcc atttttattg tgttatttct ctgtaaaatg gatagattaa tgactgtatc
     5641 tcgttcgatt gcaggattaa aaacggaata agtaattcca ttttatcaac tccaactttt
     5701 acgtcaacat tcaatacttt aattcttttt tcccacgttc tcaatgcatc gattgaataa
     5761 taaattagta aatcttttaa tattacatca ttctgctcaa acgttagata ctccaaataa
     5821 ctaccatagt attcattgta aaatcttgta ccaatacctg tgcttagaat atctttcatt
     5881 gattgtttta tactttccac atctgatact aatgctaccc tgctattcaa atcaggttga
     5941 aacgtaaatt tctcactaac acctaagtat tctaagttca taaatacaat ataatgaaaa
     6001 ttttagtcta atgtaacgac ttcagatagt atttcaatca actgtgattt tatcgtcgtc
     6061 gctcttgctc cgtattgtgc aaataacgca ctatttattg gcgttgttga cggtgaacct
     6121 ggtggattac acgttactgt catggctacc attgcatcac aaatatcttt ttgattatcg
     6181 catatttcct ctaaacgttt ttttagagtt tcacccaatg ctgctttctc ggcagaacct
     6241 ccgttcttac ccaaacttat tttttgtcct ttgatttcaa ttacctttcc gtctttgtag
     6301 ctgaagtaaa tcctgtcatt tttctcatca attacccaac tatgtccttt cggagtaaca
     6361 atgcagtaaa tgtctttgtc ggcaatctga atgctctttg acttcagcca ccaaccgtac
     6421 tcccacaccg ggaaatttac gttacctccg tcgaatgtaa gcctgataac atcaccattt
     6481 tgaggtataa agtgcatacc atagttttta cctgcaaata aaccacttgg ataaacccac
     6541 ttatcgtatt tttcattgcc gtaaattctc ggacaggaaa tttttaaacg tccacgtttt
     6601 tcaggatcgt cgcaatctac taccaatgcc tcatactttc catagtataa accgaagtat
     6661 tccaaacctc cacttgtcat gttatctaaa aattctctga aagcgctcat tatttttttt
     6721 ctttaccctc ccaattataa ctatgaattt tctttacctg ttcctgactg tttttgcctt
     6781 ttgtttcatt cacatcattg ttttggcttg tgtttgtact cgttttggca gtattattag
     6841 tcaatgattt atttgatgcg tttttatcgg cacttatgac ggtacttgca ccactaccaa
     6901 tttgatgcac cacttttgtt acataccaat taccggaatg tttttttgct actcctgata
     6961 cggtaataat atcacctgca ttaattgtcg ggtccaggtc tatacccatt tccatttgca
     7021 actgtgattt atttgcatct ttttgtgatg ctgaagttat tttatctgca gctactttat
     7081 catctaccgg aacggcaata tttttaccgg tagttattac ttcaccaata ggtgcaggat
     7141 tgcttatacc aacctcctta cctttccaat cgaatgaatg ctgaacttta cctaatgcag
     7201 tttcgggatt gcttttacta ccagctttag atgtaaactg ctcacctgtt tctttgtcta
     7261 tacctgcaac ggtaacggaa tcagattttc cctcattttc ctgactgtat gacggattga
     7321 aatactttac aatatcatta ccgtcaccat aggtaaacag tcgcttacta tctttcttca
     7381 ggttacgttt ctgaaagtat aattcattac cacgaacgta aaattcaaaa cttccgtcct
     7441 taccattact tccctctttt ttagctaact cattgcagaa ttggtgaaac gttttatttc
     7501 cctgtgatat gctcttatga acaaatgtac tttcgtcaat atttggcaca tatccgtata
     7561 aattgcagat atcgctcact atttgtgaac tcgttttatt cgtgtaaact ttattcgacg
     7621 tttccttttt catgtagaag cctgtatctc tacattttat cgtcatggaa attgttttgg
     7681 cataatccac gtctatatct ttaatcactg ccaacctttg gccggactgc acacgctcac
     7741 ctaatccgaa actgtatatc acatgattac cgacaataaa atcatcgcta tcaatcaatt
     7801 ctaatgtggc tccgtcaatt ttcaactcta ccaaatcatc ctctttgatg caatcctcat
     7861 aatgcagatt tgtaacatag ttcgttatgt tcctgccact ttcttttatc tggattatgt
     7921 aatatggact gcttgccatt attcgcttaa tttaatcaat gcaaaattcg gtattacaat
     7981 attcttacct atgtactgcc ggatatcgag aggatttttg atgttgttaa cgtctgctat
     8041 gtatttccaa tacttaggag catctgaagt aaatctactg taaaatttcc atgctaaaaa
     8101 tgtaagtgta tctcctgcaa ctactgtatg gtaaatatca ggctcttgtg gagaatactt
     8161 tattttatca cgtttcagca atttattacc acttggcaga actattatcc ggccattact
     8221 gtataagtca aaatcgtgta aatataaacc tgccatttat ctatctctta catcgtttaa
     8281 attaatattt ctttgcgtat ctaatttgaa actcactttt acttttgcca ttacaggcaa
     8341 ccaaccattt tcagcatcaa atatactcat gtcgggatct acggaatcaa ttacccaaac
     8401 gtcgttctta aacagttctc cgaatatcac tctaacattc cggtaactac caggtgctcc
     8461 gtcgttatat gtcaatgatt tcagccaatt tatcgtttta atcacatctt tcctttcaac
     8521 gtcgtcactg taaaagtcta aattcaatgt catattttca ctaccgctca catactgcat
     8581 catttcatta ttacggccta cgattaccac gtcctgcaga tttgctttcc tcggattact
     8641 gatattttca ggcacgaatt gtatcataca cctttggaac ggaggccgta attccacaat
     8701 gtataattca ctctttgttg tattgaaatt gtactgcatt attttcgaga attttgttct
     8761 ttatcaacct tatccaatgc agtttttaat tccctgccgt caatctgcaa tttaaccgtt
     8821 tgtaacgttt cttttttctc aactgtttga gtgaatgaat tagtattctc accacgaaca
     8881 cttttctgtt gagcaatagt ggtgcctatt gcagacggag tggttaacgg agttggtcca
     8941 ctaacatttc caccattacc ggtgccgttt gcttcaggaa catcaatacc caatgctgaa
     9001 agtaaaatct tacctccggg taaatcggca atcagggaaa gcatttcacc tttcaaccat
     9061 tcccacgctg aaataatacc gtcaccgatg ctctgcacaa gtgaaactcc ccaattaaag
     9121 aactgtatgc ctaatccacc caaccaaact atgaactcca taaaaataga ctttcctgcc
     9181 tccaccattg aatgccaacc tgctttgaat ttttctacca cccaatccca aattttaccg
     9241 aaattttgaa taacgtaaac gagtgcaatg attattccga taatcagtaa aataggccat
     9301 gtggccgcca atgtagcaat ggccgctcct atcattgaag ccgtccatgc tattactgca
     9361 cctaccaata ccacaccgat aataattgcc accgttccaa tcagatttcc tattgaacca
     9421 aagatcggct gaagtacgtt ccatattgca gatgccactt tacccaacat ttggaaaccc
     9481 tggcgaatgt aaccgaatgc tacacccatg ttgactgcaa actcatagat acccatgttc
     9541 tttaaagcct tatacgtgtt atcagagaac gtaaatccgt cgtcggtagc actactccaa
     9601 atttccatga acgcctgaat tacaccaccg attttttgga atacaccgga gatacctgat
     9661 agctttttac ctgtaccgtc ggccatagca ttgaagtcct caaacccttt tttcattctg
     9721 taaaacagat agataacgga tgctactgca actataattg gcaggaactc ccaaatcaat
     9781 ggcaggagtg atgccgtcaa cgctttagta cctgcaatca atccctgttc agcgaatgca
     9841 gttgcaatgg cagtcttacc caaagatgcg aatgccaatg cgagttggcc ggatgcaaac
     9901 gtggctaaat ttgtcactaa tacaaacgca cctaatacca atgcacctgc tcctataatt
     9961 ccaaacgtga tgagtaatac cttaccgagt tttgtacttg ccaaagatgt aacaatatcg
    10021 aacaaaaatt ggaaaccttt tacgagcaaa gataacggct ttgtaaatcc tgaacctatt
    10081 actatcatca tcgtttcaaa cgaacctttc atctgctctg ttgctccttt taggttattc
    10141 atacgctccg ttgcaacact caatgcagta aactttgtca tggcttgttg catatcattc
    10201 acgcctttcg caccctcttt atacaggata tttgatgctc tgattgcatc ggtaccaaat
    10261 aaagttacca ttgcagatgc tctctgctca tcggttaatc cactaaatga tttttgcaat
    10321 acatcggaga taccggctaa atctttcatc ttaccggtac tatcaaaaaa tgcattcgca
    10381 ccgtctgcag taactaatcc taattttttg gaaagttcaa tctgtgcctt tgttgtcggt
    10441 attaaattca gcaacatatt ttttaaagag gtaccaccgt ctgaaccttt caaaccattc
    10501 tgtgcaaata ctgctaatgc cgtactcgta tcaagaaacg tcattttaaa accggatgca
    10561 actgcagatg cttgtgataa acccattttc aggccactga tatcggttgc agaagcattg
    10621 gccgctcctg ctaagatgtc agccgctttc gttactgtta gcgcatcatt cctaaatgca
    10681 tttaatgcca tagaagcaat ttcggctgca tctgcgactt ctatttcacc tgcggccgct
    10741 aaattcaatg caccctctat acctccgttt acaacatctt tcaaggaaac acctgcttta
    10801 attaactcct caataccctg tgctgattct aatgcagaat actttgtttt cttacccatt
    10861 tcgagtgata aatcactaag tattttcatt tctgcattgg tagcacctga taccgctttt
    10921 attgcggaaa gtccttgttc aaattctgct gctttatcaa taggaaatgc caaagttgct
    10981 aatatacctg cacctactac cattgaagca aaaccaagtt tcattttcga taaactactg
    11041 tcaattttgt ttgctgcttg ttcagatata ccctccaaag ttttgaactt tgtggcaatc
    11101 ttatcagatg ttgcactaaa ttggtcgtac aacgtgaata caatccctgt tccgattgca
    11161 ccaccaccta cgctacctcc tccgaatatc atacttttac tttttgtgtt ttgctttatc
    11221 catttccgct ttttcatgtt ttttctgaat gtccatacgc attaaaagcg agtaaatatt
    11281 atcgtatggc attgcatata attcttcgag tgaaattgac agtcctccat aacttaaatc
    11341 gaaccacgca tccataaccc ggaatcccaa ctcgtaccct tttaaagtgc caagctgggg
    11401 aagaaaaaag ccggagtagc tatcagattt acctgcgcct gtatagatgc atcgcctttg
    11461 tattgtacca ccacagtagt atgaatatcg gcctccacct ttctcatttc accacgtaag
    11521 aactctatat caacaatcga cagttctccc aatggcaaga taattggtat ctccttgcct
    11581 tgctccaact tgtcgggatc taaatacttt gcgtcacgca tcacgatata cgtgtgggaa
    11641 cttattttat tggacttcaa tgttttgcca tagttgtttt cagtcttacc gtccaacagg
    11701 ttgtaaacta ctgtaacacc gctttcaggc agttttaatg tttgcttttt atacaacttc
    11761 agcatttcat tgtaatcctc gaataatacc gggaacggta actgcataac ttcgtttttt
    11821 tgctcgttat tcagttcgtc ttgttcaggt attccacgct cctctttgta cgaaatttcc
    11881 atagctttta acgtccattc atacggtttt ttaggaaagt cctctttgtc gaaaataact
    11941 tcatacgttt gctctagtct tttactgttt tgagtcggaa actcatactt aaaagtgaac
    12001 gtcggattaa atccgttcga caactgacgg agcgccataa gcatttcctt tctgtcttca
    12061 gacaacaggt tctgaatatc ctttaaatta ggacttgaat tacttccaat cctggtaacg
    12121 cattcaagca acatagtatc gattgcatca cgacgtttag cctcgtcatt aatcgaaata
    12181 tcctcttggt gcttacctct tagggattgc agttcaacct ccacaccgga gggaattacg
    12241 tgtgtaccac gtttacgatt tccaaaaaca ttagacataa ttataaattt ttcattggtg
    12301 aataatatca aatataaaca aaaaaaagag agatgcgaac acctctctaa acacttaaaa
    12361 aacaatatgt ctgtcagcaa gtcaaacaca tatctttata agtttgcatt tctgcaaata
    12421 ttatattttt ataccataaa caaatccaac tgtgaacctg caaccggctc aaaaaccggt
    12481 gcctcatata tcggtaaatc tacattgtgg tccataagaa cctgcgtaca taattccact
    12541 tgttctttag catcctcata atcttttggc ttaaatttaa cgccattagg gtactttccg
    12601 ctatatatat tttctaacgt cactttcata tacatatacg ctctccacgt ttctgcattc
    12661 atcatgtgac tgtttttttt gctcttttcc ataactctta attaaacatt tccatttgtt
    12721 cagcgacgtt atatttcact ctgttcagcc tccacgtcct gtttggttgg ccgtatgctc
    12781 cctcacgttt gcctggcagt ttctctaaat cttccttacc tgttgcagta agattgctga
    12841 ttgctctgcg tatagaagtg ataggaacct tacggctaat cacacccttt tttatcatgt
    12901 actcatacac ctcgcatggc gtaaagtctt tcaatcggtt gtccttgaat acctgaagta
    12961 cgagaacgtc ctgtttttgt gccttttgtt cggctgcttt taactccggt ttttgcatac
    13021 ctgttgtatt gtggtatgaa tgcatactgc taccgttttg ttgacttaat actgctttca
    13081 ttgttgctaa ttttagtgtt ttataaattt tgtttcaata gcatccgcta accacaaatc
    13141 atgctcctca tttactccga taatttctat cgaaaataat tcgtctttca ggtgcaatat
    13201 tgcctgacga acttctgtga aatactctgc aaattcagag gtatcgacct tttctacgaa
    13261 tagaattttg tcgctttcgg taaacatttc gtcaatggca tccgtactac cgtctattaa
    13321 attgcctatc agtttgacgt gtttagtaat atcaaaccct atatctgtaa actgcagatt
    13381 tgatttatcg atattcggat tgaataccgg tgctccgtcg attatagaga atgctacttg
    13441 tagcttcgct cttaattcgg gtgccaggtg cagtaagata tgacgattat tgtccaaact
    13501 atctttctgc cataccataa ctaaacagta atttgatttc gttgttgctt ttaatccgta
    13561 catgattttt tatttaaagt tagaaaaata tttcgagtgt gcactcttgc acacccgaaa
    13621 tttaatactt tttagttcaa ctgtgataat tcagcaaccg tcttacgtcc tctctttttt
    13681 atcggtgtat gtgtatagga cttcatttgc ttccacttta tattctccgg ttgccttatc
    13741 tccgtttttg gctgtgcatc cttcaaatcg tgcgctgtgt ccactttctc cgaatacctt
    13801 tataaagtct ttgattttca tgtcttaaat atttgtatgt gaaattttta aaatactttc
    13861 cttttcttcc caatgttcca atattatttc ccattgcgtg ttgttgagta tgaaatttgc
    13921 tgataccagg aacgcaaagt tttcatagtt atctccataa agaaattgag ttgcatagat
    13981 cggatatatt atcttataga aaatatcctc cgtgctttgt gagtagtcaa attttttgat
    14041 tttcattttc ttaaatttag attgttaaaa attccgttac tgccttgcgg attcgttgaa
    14101 atgtgttttg tttttactgt tcggttaata gttttatcgc atcacgaata aaccttgcta
    14161 catcttcacc accgattaga tagttcccgc ttgaatcgta catataagcc gaaaattcgt
    14221 ttgaagttct gttgtttaat tgaaaatctt tctcattctc aaaaatgaaa ttgataatcg
    14281 cctgttttaa ttcctttttc ataaaatagt ttttagtttg ttattaaatt gcttgtgtag
    14341 gttgtgaatg ttccgtactt tttcaccatt tcggcggtaa aatcttctaa cggcgtgctg
    14401 gtgctgtata tttccgtttt tagtccttgt gtagttaata gcacgatttt atatttttct
    14461 gctgtgttct gcatcttgtt tagtttaatg tgatttgtaa agcggttctt tttataaagt
    14521 ctgggttgtc ggttgcttgc tgataaaaag cagtatattc tgccgtatct ataagaccac
    14581 aagtgttata ttcactttta aaagtaggtc catatacggt tccttctttc acatatccag
    14641 cacttaaaaa accacaacca ggaaataaat taaaaaagtt ttcaatatca ggtccggatt
    14701 gataagagaa aacaacgccg ttaacgtttt taaaagatat tgcgtgctgg ttaatttcca
    14761 ataacataaa taacccttta tatttgccgc tgaataactt ttttgccgtt tttttgatag
    14821 tatgtgataa cataagtaat tttttagtga tttgattaaa atagtttggt taattaaata
    14881 gtttttcttc tgtgtcgtta taatggaatg ttcttaccat gttatgcttt accggaactg
    14941 gattaaaatt taatgctacg gttaatcctt ctttgtgtgt tattgccttt cctgtttctt
    15001 tttccaacat tttcgctatg ttagctgcaa tttcacaaaa tatggttttg gtagtttcat
    15061 cttctctttt tattaaagag tgtattgtta ctccattttt atacgtttct gcttcaatat
    15121 agcatccttc attttgtatt ttagctttaa ttgagttgta gttattcata aaatcttttt
    15181 tagtttgtta gttaattttt cctgctttat gctgtggagt taaaaattga tgtttataat
    15241 tctataaata aatttgtata acatgggtta aatggactgt cttgtctgtt tatttcatta
    15301 aagaatttac ttagtccgtt tattcctccg gctgccttta ttgttccttt taaaatcttg
    15361 tatgtaaatt cataatcatt tataacactg tattctttac cacttggaaa tattactttt
    15421 tctttttcaa agtcaatttt tattattcct tctcttaatt tctgcccgtg tatgttgtaa
    15481 gaaattaaat ttatttcttg tgctgtggtt gctgttgctg tattcatttt attattttta
    15541 ctttgtgatt aaatcgggtt tataaataaa gaatttaatt gtttctgctg taaagtttcc
    15601 cgctttcaga aagtcgttaa attctgcgtt aaactttgcg ttaaattcat ctaaaaattt
    15661 agggttgtat tcatttttac tttgccatgt tttaataaag tctaaaactt tgttattttc
    15721 attaatggct gtttgtactt cttgtgatgg tggtgtttgc atggtgttta ttttttaaaa
    15781 taattgtaat tgatttttat ctgtttcctg tgttacttcg atttgtggcg tgtattcgct
    15841 tatgcttcaa atacttcctt tgttgtcatg tcgtttattt ttgttgttgt tttaatttga
    15901 tagtgtaaat ctatgcagga tatttgcttt gtgcaatatc ccacaccatt ataatactat
    15961 tattttcaaa ataaaaatgt aaatactctt atttcaatct gttatgaact gttactaaaa
    16021 tgttaaggaa tttaaagaaa ccattttcag gcttgaaaat aggctgattt tttctctatg
    16081 tagtttttat taatatttct gaatacgata aatctgcatt ggtattcagg ctacatctta
    16141 tatctctctt ttttttgagt ggaggatata aaataaaaaa acccgactat aaaaatagtc
    16201 aggctttttc accaataaac acttcctgcg tattactgta cgttagtatc gcctacgctg
    16261 aaagttacgg ttcgtataag gttatcggag ttatctcctt tcgtttcata attagcggtc
    16321 tcc
//
LOCUS       DAOOOI010000048        16274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_715_length_16274_cov_20.196745, whole genome shotgun sequence.
ACCESSION   DAOOOI010000048 DAOOOI010000000
VERSION     DAOOOI010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 16274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16274
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_715_length_16274_cov_20.196745"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1761
                     /locus_tag="PKX59_07205"
     CDS             <1..1761
                     /locus_tag="PKX59_07205"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HOZ90692.1"
                     /translation="DKGSLKQKIGDYYYSGMDTVSIDKAGIAPLQEYFTEIDQMKTFD
                     DVMKVAAHIHRISASPLFGLYVNSDDMISSKNAVFLSQGGLGLPDRDYYFNTDERTKK
                     VREAYPEHLKKMFELSGFDEKKATDAAADVYAVEFALAKVSRKMEDLRDPFKNYNKTS
                     VKELQEKLNNVPMQTFFSGLGMNLPDTVIVGQPEFFTYLNLASRQFGIEVWKNYLRWH
                     LLRGLAPYLNTDIEKQNFSFYGTLLNGVKEQRPRWKRVVEDTDGQLGELVGQIYVEQY
                     LPKGTKEKLMEIGNAVKEVYRKRISGLDWMSDATKTKAITKLDAMIMKVGYPDKWKDM
                     SALEVDKASYVGNVIRANSWHFDYMKNKYGKPVDRTGWNMTPQTYNAYYNPSNNEIVV
                     PACNITVPGYEKTLADDAILYSIIGGSTFGHEFTHGFDDQGSKYDQFGNLNNWWTSED
                     SIRFYAKTGAVVNQYSHYLVVDSLYINGDMTQGENIADIGGVAMGLEAFKQTKQFKEN
                     QIIAGLNPMQRFFLGYAYAWMVNMRPEALATRVLTDVHSPAKFRVIGPLVNMPEFYTT
                     FGVKAGDKIFLPENERVHIW"
     gene            1789..2508
                     /locus_tag="PKX59_07210"
     CDS             1789..2508
                     /locus_tag="PKX59_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOZ90693.1"
                     /translation="MREESIKYYSPIIGRDFEMLIFGESGIPVIAFPTSMGRHYQNRD
                     FKLIESVAPFVESGFIKIYCPDSIDEQSWYNTHIHPADRVRNHLLYDQMILEEVVSRA
                     QKETSHNKVITAGCSFGGYQAINFAFRHPEVVAHTFSMGGAFDIKMHLDGYYDDNVYF
                     NNPPDYLPDLDNEHLFQMGIVLGVGEHDFCIAQNKRLSEIMTKKGMAHWLDIRPGANH
                     DWPVWREMFPAYIEALQTAKV"
     gene            2789..3592
                     /locus_tag="PKX59_07215"
     CDS             2789..3592
                     /locus_tag="PKX59_07215"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOZ90694.1"
                     /translation="MENQKDKSEIEDVREDVWRRFEKDNQRGKLFGGLIIICVGALLF
                     AREAGFYFPSWIFSWPMILIVIGIFLGVKHAFRNMAWMVLVLIGGLFLIRNSFPIFEY
                     RNFIWPVVIILLGLFIILKPSNRFRRACRRGRFHPRHPQRQFGNYTGTPSSGEDFLEA
                     VAVFGSIKKNIISKDFKGGEVSAVFGGAEINFMQADIQGRVELEVNQVFGGTKLIVPA
                     HWEIKSEIAAVLGSVEDKRPLRSASLTDEKKVLILRGTTVFGGIDINSY"
     gene            3628..3966
                     /locus_tag="PKX59_07220"
     CDS             3628..3966
                     /locus_tag="PKX59_07220"
                     /inference="COORDINATES: protein motif:HMM:NF025484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4288 domain-containing protein"
                     /protein_id="HOZ90695.1"
                     /translation="MYFIAKVIYQIVINGSKNNQFDEQLLIIKAQTVADAYLKARAIG
                     AEKQHEFINDRKNPVMWKMIDVADIIPVESPEHGSVVYSQVIEEDSESCIYYIKRKAL
                     TLQTQSLLFA"
     gene            3973..4998
                     /locus_tag="PKX59_07225"
     CDS             3973..4998
                     /locus_tag="PKX59_07225"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOZ90696.1"
                     /translation="MIIEKIKNSIDQIVWWLCWYFFVAALVHQFGFDWCLSFADSTVM
                     LLLLLLTSCSTYFAFNKFQKTGNRFFVILWNIALSWISVFILGLVLRYIYVDDLKYLT
                     FLDSSMVVRFTWFFIMNLLVTVIYGLQVNILEQKKLEAHNNENANLMKDAELSGLRLQ
                     LQPHFLFNSLNSINALIGTEPAKARSMVLQLSEFLRGTLQRDNKKLISLQEEISHLKI
                     YLEIEKVRFGHRLITQIEIPENCLNMMVPPLILQPIVENAIKFGLYDTTGQVTISLKV
                     SCMESHLRIFVANPFDSDTAASQKGTGFGLDAVQRRLYLLYARNDLLKTEKENSIFIT
                     QIDIPQI"
     gene            4995..5729
                     /locus_tag="PKX59_07230"
     CDS             4995..5729
                     /locus_tag="PKX59_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HOZ90697.1"
                     /translation="MIKAIVIDDEPLARSLVLEFLKAFPNIEVVQECNDGFEGLKAIQ
                     QFQPQLIFLDIQMPKINGFEMLELIENPPGVIFTTAFDNYAIKAFEAHAIDYLLKPFS
                     EERFNKAVAKFLATNNKAPAMDLVSAESNILPDEKNRIVVKDGSKITIIPVDDVIYIE
                     AYDDYVKVHTQNGFKLKKKTMSYYENTLDNSKFLRVHRSYIVKLSMITRIEQYEKTSH
                     IALLKNGQKVPLSKQGYARLKSVLGI"
     gene            complement(5731..6132)
                     /locus_tag="PKX59_07235"
     CDS             complement(5731..6132)
                     /locus_tag="PKX59_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90698.1"
                     /translation="MLYIICFSAVNCQAQCLKLKTNITAEDNLYDSIVVVIADESGNV
                     NRLNTTKENRLKLKLNNHYLITIIKPDAVAYKFEVNTHLPVKLSKKNYSYSTNIFLEK
                     LNPADANAQKEKACKTRMTISFNKDKKAFGF"
     gene            complement(6144..6875)
                     /locus_tag="PKX59_07240"
     CDS             complement(6144..6875)
                     /locus_tag="PKX59_07240"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOZ90699.1"
                     /translation="MTACKLSVNINKIATLRNARGGTIPNVVKAAIDIERFGAEGITV
                     HPRPDERHIRFRDVYELKECVTTEFNIEGYPSEGFLKLVESVKPHQVTLVPDSPDVLT
                     SNAGWNAIREQAFLKNVIERLKQKCLRVSLFMDTDLKQIDAAATTGTDRIELYTERYA
                     SGYEKSAQQAIADYQKAASHASTLNMGVNAGHDLNLNNLNYFIKNIPQVIEVSIGHAL
                     ISDALYFGLQNTVQMYKHQIKSARE"
     gene            6941..7603
                     /locus_tag="PKX59_07245"
     CDS             6941..7603
                     /locus_tag="PKX59_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOZ90700.1"
                     /translation="MNTKAYIKDFIPPLKSSDTVVRALQWMQAFNLNHLPVVDGVKYK
                     GLITEYDLKKIEDKEKSLSECELEYQNVYIFETQYIYDALEFVTVKGLNIVPVVSKEG
                     NYRGLLTLVDIIDCFAQNSSVKTPGGIIILNVERKNYSMQEIARITESNGALILSSSV
                     NQLPDSEIFEVTLKVDKLDLSRILAGFYRMDYNVVASYQTTDLPTDIEDRYQAFMTYL
                     NV"
     gene            7596..8999
                     /locus_tag="PKX59_07250"
     CDS             7596..8999
                     /locus_tag="PKX59_07250"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOZ90701.1"
                     /translation="MFKRAFLINLFFAFCFSSFASDSLQVDTNTIYVRQIVVTGNKVT
                     RYSVIQRELAFSQGQTMTVGELNEKITKSRENLLNISLFNFVNINKQLIGNDVYVIID
                     VVERWYIFPVPIFEVVDRNFWEWWRDKNLKKANYGFYLNWENFRGRRENVRLLLRYGY
                     SQRQGFMYSIPSIDKSQNNGLVFTALQTRMREIPYKLENNKLVYFENGSEFLRKEYIA
                     NVRFIHRQGFNKTYTFTGGYQYNIISDTLLSLNRDYFVSGHKYESYPVISFEYKDDAR
                     DIRNYPLKGYFLSLEFTKYGFGISDNDPSITYIIAHFKKFWKLNDSWATAFSFKGKLS
                     GNNDVPYYNQIALGYRGDLVRGYDPYVINGENFALFKSGIKYAIVQPKSFNIPVGLPQ
                     SFTRIPYALYANLFFDAGYVRDKRFYQNNPLSNKWLYSFGAGLDFVTYYDLVLRGEFA
                     FTGYGKSGIFLHLTAPI"
     gene            9012..9893
                     /locus_tag="PKX59_07255"
     CDS             9012..9893
                     /locus_tag="PKX59_07255"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOZ90702.1"
                     /translation="MTIAVYARNVNEKVLAALDVVWTQLHQNDVDVLLYGTLYRHLKK
                     SGKEMQNTHVFSTSAEIAGKADFLFSIGGDGTILDTIKLVKDSGIPVLGINTGRLGFL
                     SIVLPEAITEAINSILKGHYSLDKRAMLRLETDKEIFGDVPYALNELAIHKKDSSSMI
                     IIHAFLNGEYLNSYWADGLLIATPTGSTGYSLSCGGPVISPGSGNFVITPIAPHNLNV
                     RPIVVSDKNVVSLEVEGRNPYFMASLDSRSVTIDSSVQLAVRREDFSFNLVRFDNDNF
                     LQTLRNKLNWGLDARNW"
     gene            9960..10847
                     /locus_tag="PKX59_07260"
     CDS             9960..10847
                     /locus_tag="PKX59_07260"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOZ90703.1"
                     /translation="MNDKYKKLLLVGLILIPFLGSAQIAKKRWKAYRIEYIAGLGATN
                     FLGELGGANRVGSHAFRDLEFSMTRPAAMIGYRYKLSPTFAWCSKFSYGIVSGDDALT
                     KEHFRNNRNLSFKSNIFELSTNIEISFLQEQVGHRYKLRGVRGLRNVEISAYGFLGIG
                     VFHFNPKAKLDGQWYQLQPLGTEGQGQVASRSKYKRTQLCVPLGLGAKYAIDRQISVG
                     LELGLRYTFTDYIDDVSKSYYYDRGGSLVEILADRSHDDNSHVGEQRGDPRWNDAYVF
                     AMFNICYKIRTGRVNYPMF"
     gene            10894..11742
                     /locus_tag="PKX59_07265"
     CDS             10894..11742
                     /locus_tag="PKX59_07265"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOZ90704.1"
                     /translation="MRKILILFFTIVVLQPQKISAQSSELGLLLGVASYKGEINTHLF
                     NPNAFNPAAGLYYKRCLNSFWSFRLGLTYGQITGSDAIADEDFNQYRNLSFRSDIWDA
                     SALFEFNFFPFQTASEHTSKVAPYLVAGITFFHHNPQGFINGSWYDLQPLGTEGQGSG
                     LPNTTGKYKRVQFAFPMGGGIKFKLSNRFSLSVEAIARRTYTDYLDDVSTVYPDQAEL
                     RNTNGVLAAELSDRTIFAGTDINAKRQRGNASDKDWYMMGGFTINWTLSKKYTDKCKP
                     FRTKLR"
     gene            11813..15178
                     /gene="mfd"
                     /locus_tag="PKX59_07270"
     CDS             11813..15178
                     /gene="mfd"
                     /locus_tag="PKX59_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOZ90705.1"
                     /translation="MDLLKDLKEKYIAHPAVQELFLQISKQASKTHLQNTAGSASSFI
                     ATATFEHEPAPMLFILPDREEAAYFLNDLENLNPEQKVFFFPSSYRKPFKLNAHENAS
                     IQMRTEVLSHINKHSQSFIVVSYAEAVAENVVTRHALEKNTIEARVNEQLSIDFVNDF
                     LTENNFQRTDFVFEPGQFAIRGGIVDVYSYAYELPFRLEFDGNKIESIRSFDPVTQLS
                     EKNLHFVTIIPNIQTEMQQNVTESFFKFLPENTLIWTRDLSYSLNYLDDNLTVLKNQT
                     SENDEEEKSDNPRIVYDSIEEIKSVLSTFRQVEFGLKSFYENVVGIKFNIQPQPQFNK
                     NFNLLAGHLKENSDKHFKSYIFSDSAKQVERIYAIFDDLSFKKEVQRDDIQFSPVYTS
                     LYKGFTDLDLKVSVFTDHQIFDRYHRFKLRKNYSRNEAISIKELYSLKPGDYVTHIDH
                     GVGRFGGLEKLNIEGRQQEAIRLVYKDNDILYVSIHSLHRIARYTGKEGTAPSLNKLG
                     STSWASLKQKTKKKVKDIARDLIALYAKRKAQEGFAYTPDNYLQTELEASFIYEDTPD
                     QIKATRDVKKDMESASPMDRLICGDVGFGKTEIAIRAAFKAVNDSKQVAILVPTTILA
                     MQHYRTISERLKDFPCTIDYINRFKSTAQQNETLRKLEVGKIDIIIGTHRLLSKDIKF
                     SNLGLLIIDEEQKFGVASKEKIKSLKVNVDTLTLTATPIPRTLQFSLMGARDLSIINT
                     PPPNRYPVTTELHTFNDKVIREAIMYEVSRGGQVFFVHNRVQDIHDISDMIRKLCPDV
                     KIVVGHGQMDGSQLEEVLMTFIEGDADVLVATTIIESGIDISNANTIIINQAQNFGLS
                     DLHQMRGRVGRSNKKAFCYLLAPPLTVLTPEARQRLQAIEEYSDLGMGFQVALRDLDI
                     RGAGNLLGGEQSGFISEIGFEMYQKILDEALIELRESDFKELFPADDNKPIVADCQIE
                     TDLEILIPTQYVENIVERLNLYKELDDIETEEKLNAFESQLRDRFGPVPKQVMELFET
                     IKLRWKAKSLGFEKLILKNGQLKGYLVSNPDSRLYKSDLFMQIINFVKSNPSRCRMKE
                     DKSKLSLTFYKIETVNEANAVFRQMMKPSVATA"
     gene            15209..15280
                     /locus_tag="PKX59_07275"
     tRNA            15209..15280
                     /locus_tag="PKX59_07275"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15242..15244,aa:Arg,seq:ccg)
     gene            complement(15357..>16274)
                     /locus_tag="PKX59_07280"
     CDS             complement(15357..>16274)
                     /locus_tag="PKX59_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOZ90706.1"
                     /translation="GTEESLQIIRERMDEIAAVMIEPIQSRRADFHPKEFIHEVRKLT
                     KENNVLMIFDEVITGFRLRLGGAQEYYGVDADLSTYGKVIGGNMPVGAIAGKKQYMDA
                     LDGGFWQYGDNSIPEVGVTYFAGTFVRHPLAMAAMKEVLLHLKKEGKPLYEKLNAKTQ
                     RLVDEVNAHSKKLGSPFKLVTFGSLFKGKWETEPHYTELLFAMMRYKGVHIMDGFPCF
                     LTLAFTDADVDFVVSKFKESLSELCEIGFIPSDKNNEQSKINSNGHLQNGNGQHPPIP
                     GARLGKDANGKDAWFIADPERPGKFLMVK"
BASE COUNT         5148 a         2738 c         3252 g         5136 t
ORIGIN      
        1 gacaaaggaa gtctgaaaca aaaaattggt gattattatt acagcggtat ggacaccgta
       61 agtattgaca aagccggaat cgcgccactg caagaatatt ttacagagat agatcagatg
      121 aaaacttttg atgatgtgat gaaagtggca gcacatatac atcgcatttc tgcgtcaccg
      181 ctttttggtc tttatgtaaa ctccgatgat atgatcagtt caaaaaatgc tgtattctta
      241 tcgcaaggtg gattgggttt acctgatcgc gattattatt ttaataccga tgaaagaaca
      301 aaaaaagtaa gagaggcata tcctgaacat ttaaagaaga tgtttgaatt gagtggcttc
      361 gatgaaaaga aggcaacaga tgcagcagct gacgtttacg cagtagagtt tgcattggca
      421 aaggtcagcc gtaagatgga agacttgcgc gatccattta aaaattataa taagacatca
      481 gtaaaagaac tgcaagaaaa attgaacaat gtaccaatgc agacattttt cagcggttta
      541 ggaatgaatc taccagacac tgtaattgtc ggtcagcccg agttttttac ttatctgaat
      601 cttgcatctc gtcagtttgg aattgaagtt tggaaaaatt acctacgttg gcatttgtta
      661 cgtggacttg ctccttactt aaacactgat attgaaaaac agaatttcag tttttatggg
      721 acactgttaa atggtgtaaa agaacaacgc ccaagatgga aaagagtagt tgaagatacc
      781 gatggtcagt taggagagtt ggtaggacag atttatgtgg aacaatatct tccgaaagga
      841 actaaagaaa agttgatgga aattggaaat gccgttaaag aagtttatcg taagcgcatt
      901 tcaggacttg actggatgag tgatgcaaca aaaacaaaag caattacaaa actcgatgcc
      961 atgattatga aagtgggcta tcctgataag tggaaagata tgagtgcatt ggaagttgac
     1021 aaagcatctt atgtaggtaa tgtgattcgc gccaattcct ggcattttga ttatatgaaa
     1081 aataaatatg gcaaacctgt tgaccgcaca gggtggaata tgacaccaca gacttataat
     1141 gcatattaca atccatcaaa taatgaaata gttgttccag catgtaacat aacagtgccc
     1201 ggatatgaga aaactttagc tgatgatgca attctttaca gcattattgg cggaagtaca
     1261 tttggtcatg agtttacaca tggctttgac gatcagggta gcaaatatga tcagtttggt
     1321 aatctgaata attggtggac ttctgaagac agcattcgct tttatgcaaa gacaggtgct
     1381 gttgtgaatc agtacagcca ttatcttgtt gttgacagtt tgtatataaa tggtgatatg
     1441 acacaaggcg aaaacattgc cgacattggc ggtgttgcaa tgggacttga agcattcaag
     1501 caaaccaaac agtttaaaga aaatcaaatt atagcaggac taaatccaat gcaacggttt
     1561 tttctgggct atgcctatgc atggatggta aacatgcgtc cggaagcatt agcaacaaga
     1621 gtgcttactg atgtacactc acctgccaaa ttcagagtta ttggcccttt ggtaaatatg
     1681 cccgaatttt atactacctt tggtgtaaag gcgggagata aaattttcct tccggaaaat
     1741 gagcgtgtac atatatggta aattattaat tttgaaaaaa cttgtgtgat gagagaagaa
     1801 tcaataaaat attattcgcc aatcattggg cgcgattttg aaatgctcat tttcggagag
     1861 agtggcattc cggtaattgc atttccaaca agtatgggca ggcactatca aaaccgcgat
     1921 tttaaattga tagaaagtgt tgcccccttt gtggaaagtg gatttattaa aatttactgc
     1981 cccgatagta ttgatgagca gagttggtac aatacacaca ttcatcctgc cgaccgggtg
     2041 cgcaaccatc ttctttacga tcagatgatt ttagaagaag ttgtaagccg tgcacaaaag
     2101 gaaacttcgc ataataaagt aattactgcc ggttgtagtt ttggtggtta tcaggcaatc
     2161 aactttgcct ttcgtcatcc tgaagttgtt gcacatactt tcagcatggg tggtgctttt
     2221 gatattaaaa tgcatcttga tggttattat gatgacaatg tttatttcaa taacccaccg
     2281 gattatttac ccgatttgga taacgagcat ttgtttcaga tgggaatagt tcttggtgta
     2341 ggcgaacatg atttttgtat agcgcagaac aaacgccttt cagaaattat gactaaaaaa
     2401 ggtatggctc attggctcga tattcggccc ggtgccaacc acgactggcc tgtgtggcgc
     2461 gaaatgtttc cggcatacat tgaagcattg cagacagcta aggtttagat ttttttgaga
     2521 gttgatcaaa cattgttgac aggagtgatc aagtgaggag tgatgaggtg acgaagtcaa
     2581 tttgataatc agatacggct gacaagtttg ataattctga attcagaact ctacattttg
     2641 cattgatttc ctttaccgac actagcctgc cgtttaccga tatatttttc tattcactga
     2701 gtttgtagct gctatagacc tattgtgtct gtgaactacc tttgaagtca acatatcttt
     2761 gtccaataat atttaaagaa ataataaaat ggaaaatcaa aaagataaat ctgaaattga
     2821 agacgtgaga gaagacgtat ggcgcagatt tgagaaagat aatcaacgcg gtaaattgtt
     2881 tggtggctta ataataattt gtgttggtgc attgttgttt gccagagaag ccggttttta
     2941 tttcccgtca tggatttttt catggcctat gattttgata gttataggga tatttctggg
     3001 tgtaaaacat gcattccgaa atatggcatg gatggtatta gtacttattg gcggattgtt
     3061 tttgatacga aacagtttcc cgatttttga atatagaaat tttatctggc ctgttgttat
     3121 aattctactg gggttgttta ttattcttaa accttctaat cgttttcgca gagcttgcag
     3181 gcgaggcagg tttcacccaa gacatccgca aaggcaattt ggaaactata ccggtacgcc
     3241 atcttcaggt gaagattttt tggaagcagt cgcagttttc ggaagtataa aaaaaaacat
     3301 cataagtaag gactttaaag gtggtgaagt gtcggctgtg tttggtggtg cagaaattaa
     3361 ttttatgcaa gccgatatac agggtagagt agagctggag gtgaatcagg tttttggagg
     3421 aacaaaactt attgttccag cacattggga gattaagtca gaaattgctg cagtgttagg
     3481 aagtgttgaa gacaaacgtc cattgcgtag tgcatcgcta acagatgaaa agaaagtact
     3541 gatattacga ggtactactg tatttggagg aatagacatt aatagttatt aaattttaaa
     3601 ttcatttttt aatcaaacta aattgaaatg tattttatag ctaaagtaat ttatcagata
     3661 gtaatcaacg gaagtaaaaa taatcagttt gatgagcaac ttttaatcat taaagcacaa
     3721 actgttgccg atgcttattt aaaagcacgt gcaattggcg cagagaagca acatgagttt
     3781 attaatgata gaaaaaaccc tgttatgtgg aaaatgattg atgttgcaga tattatacct
     3841 gttgaatcac ccgagcatgg ttctgttgta tattcgcagg ttattgaaga agactctgag
     3901 tcgtgtattt attatattaa acggaaagca ctgacacttc aaacgcaatc acttttgttt
     3961 gcctgataaa taatgattat tgaaaaaata aaaaattcaa ttgaccagat tgtttggtgg
     4021 ctatgctggt atttttttgt tgcagcattg gttcaccaat ttggatttga ctggtgcctg
     4081 tcttttgcag acagcactgt aatgttacta ttattgttgc ttacttcgtg cagcacctat
     4141 tttgcattta ataagtttca aaaaacagga aatcgttttt ttgtgatatt atggaatatt
     4201 gctttatcgt ggatttctgt ttttatatta ggcttggtgc taaggtatat ttatgttgat
     4261 gatttaaaat atttgacttt tctcgattca tcgatggtgg ttcggtttac atggtttttt
     4321 ataatgaatc ttttagtaac tgtcatctat ggtttgcaag taaatatttt ggagcagaaa
     4381 aaattagaag cacataataa tgaaaatgcc aacctgatga aagatgcaga attatccgga
     4441 ttacgattac aacttcaacc acatttttta ttcaacagcc tgaattctat taatgcatta
     4501 ataggaacag aacctgcaaa ggcaagaagt atggtgttac agctttcaga atttttacgc
     4561 ggcactttac aaagagataa taagaaattg ataagccttc aggaggaaat cagccatctg
     4621 aagatttatc ttgaaattga aaaagtgcgg tttggtcacc gattgattac gcaaattgaa
     4681 attcctgaaa actgtttgaa tatgatggtg ccaccgctta ttttacaacc tattgtagag
     4741 aatgctatta aattcggact ctatgacacc acaggtcagg tcacaatttc attaaaggtt
     4801 tcctgcatgg aaagtcattt aaggatattc gttgccaacc cttttgattc tgacactgct
     4861 gcatcgcaga agggcaccgg ctttggcctt gacgcagtgc aaaggcgatt gtatctgctt
     4921 tatgcacgta acgatttact taaaacagaa aaagaaaata gtattttcat tacacaaata
     4981 gacattcctc agatatgatt aaagcaattg tgatagatga tgagccttta gcaagaagtc
     5041 ttgtgttgga gtttcttaaa gcgtttccaa acattgaagt ggtacaggaa tgtaatgatg
     5101 gctttgaagg gttgaaagcc attcagcagt ttcaacctca acttattttt ctggatatac
     5161 aaatgccaaa gattaatggt tttgagatgc ttgagttgat tgaaaacccg cccggtgtaa
     5221 tttttacaac tgcatttgac aattatgcaa ttaaagcttt tgaagcgcat gccatagact
     5281 accttttgaa accattcagc gaagaacgat ttaacaaggc tgttgctaaa tttcttgcaa
     5341 ccaataataa agcacctgca atggatttag taagtgcgga aagcaatatt ctgcctgatg
     5401 aaaagaatag aattgttgta aaagatggaa gtaaaattac gatcattcct gttgatgatg
     5461 taatttatat tgaagcttat gatgattatg tgaaagttca tacacaaaat ggttttaagt
     5521 tgaagaagaa aaccatgtcg tattatgaaa acactttaga taattctaaa tttttgcgtg
     5581 tgcacagaag ttatatcgta aagcttagta tgattacccg tattgagcag tatgaaaaaa
     5641 catcacacat agcattactt aaaaacggac agaaagttcc attgagcaaa caaggttatg
     5701 caagattaaa atcggtttta ggtatataat ttaaaaccca aaagcttttt tgtctttatt
     5761 aaagctaata gtcattcttg ttttacaggc tttttctttt tgtgcattag catcagcagg
     5821 atttaatttt tccagaaaga tattagtgct gtaagaataa tttttcttgc ttaatttaac
     5881 cggaaggtgt gtgttgactt caaatttgta cgcgacagcg tcaggtttta taatagtgat
     5941 caggtagtga ttgtttagtt ttaatttaag tctgttctct tttgttgtat tcaagcggtt
     6001 tacatttccg gattcatcag caattacaac aacaattgaa tcatataaat tatcttcagc
     6061 agttatgttg gtttttaatt tcaggcattg cgcctgacaa tttactgcag agaaacaaat
     6121 gatatataac atacatattc taatcattca cgtgcagact ttatttgatg tttatacatc
     6181 tgtaccgtat tttgtaaacc aaagtacaat gcatcactaa tcaaagcatg accgattgat
     6241 acttcaataa cctgcggaat attttttata aaataattaa ggttgttcag atttaggtcg
     6301 tgccctgcat ttacacccat gtttaaagtt gaagcatgtg atgctgcttt ttgatagtcg
     6361 gcaattgcct gctgagccga tttttcatat cctgatgcat aacgttctgt ataaagttca
     6421 attctgtctg tgcctgttgt tgccgctgca tctatttgtt tcaggtcagt atccataaat
     6481 aacgaaacgc gcaggcattt ttgtttcaag cgttcaatta catttttcaa aaatgcttgt
     6541 tcacgaatag cattccagcc ggcattggaa gtcagcacat caggagaatc gggtaccaag
     6601 gtaacctgat gtggctttac cgactcaacc aatttcagaa aaccttctga tggatagcct
     6661 tcgatattaa attctgtggt aacacattct ttcaattcat aaacatctct gaaacggata
     6721 tgcctttcgt caggacgtgg atgcactgtt attccttcgg caccaaaacg ctctatatct
     6781 attgccgcct taactacatt tggaatagtt ccgcctcggg cattgcgcag tgttgcaatt
     6841 ttattaatgt tcacactaag tttacatgct gtcatatact ctgaaaattg atacaaatgt
     6901 actatgtttg ccggcagatt cgatttggga aaaaatattc atgaacacaa aagcttatat
     6961 taaagatttt attcctccgt taaagagttc cgacactgtt gtcagagcat tgcaatggat
     7021 gcaggcattt aatttaaatc acctgcctgt ggttgatggg gtaaaatata aaggcctcat
     7081 aaccgaatac gatcttaaaa aaattgaaga caaagaaaag tcgctttcag aatgtgaatt
     7141 ggaatatcag aatgtttaca tttttgaaac gcaatatatt tatgatgcat tggagtttgt
     7201 aacagtaaaa ggtctgaata tagttcctgt tgtcagtaaa gaaggaaatt atcgtgggct
     7261 tctgacctta gttgatatta ttgattgttt tgcacaaaac agctctgtta aaacacctgg
     7321 tggaatcatt attcttaatg ttgaaagaaa aaattattct atgcaggaaa ttgcacggat
     7381 aactgaatcg aacggagcat taattttaag ttcatcagta aatcaacttc cggacagtga
     7441 aatttttgag gttactttaa aggttgataa actggatctt tcgcgaatac ttgcaggatt
     7501 ttatcggatg gactataatg tggtggcatc ttaccaaact acagacttgc ccactgatat
     7561 tgaagaccgt tatcaggcat ttatgacata ccttaatgtt taaaagagca tttttaataa
     7621 atctgttttt tgcattttgt tttagcagtt ttgcttccga ttctttacag gttgatacca
     7681 atacaattta tgtaaggcag attgttgtca caggaaataa agtaacacga tattctgtta
     7741 ttcagcgtga gttggcattc agtcaggggc agaccatgac tgttggtgaa ttaaatgaaa
     7801 aaatcacaaa gagcagagag aacttattga atatttcgct ttttaatttt gtaaatatca
     7861 acaagcagct aatcggaaac gatgtttatg tgataattga tgttgttgaa cgttggtata
     7921 tttttcctgt acctattttc gaagttgttg accgtaattt ctgggaatgg tggcgcgata
     7981 aaaatctgaa gaaagccaat tatggttttt atttaaactg ggaaaatttc agaggcagga
     8041 gggagaatgt aaggctgttg cttcgttatg gttattcgca aaggcaggga tttatgtaca
     8101 gcattccaag cattgataaa agtcagaata atgggttagt gtttactgct ttgcaaacaa
     8161 gaatgcgcga gattccctat aagttagaaa ataacaagtt ggtttatttt gaaaacggaa
     8221 gtgaattttt aagaaaagaa tacattgcaa acgtaagatt tattcaccgg cagggattta
     8281 ataagactta tacttttacc ggaggttatc agtacaacat tatctccgat acattgctgt
     8341 cgctcaacag agattatttt gttagtggac ataagtatga aagttatcct gtaatttctt
     8401 ttgagtataa agatgatgcc cgcgatatca gaaattatcc tttaaaaggt tactttttaa
     8461 gtcttgagtt tacaaaatac ggttttggca tcagcgataa cgatccttcg ataacttata
     8521 taatagcaca ttttaaaaaa ttctggaaac tgaacgattc atgggctact gcattttctt
     8581 ttaaaggcaa attatcagga aacaatgatg ttccttatta taatcagata gctttgggct
     8641 acagaggcga tttagtcaga ggttacgatc cttatgttat caatggtgaa aatttcgccc
     8701 ttttcaaaag cggaattaaa tatgctattg ttcaacctaa atcatttaac attcctgtgg
     8761 gattacctca gagtttcact cggatacctt atgcattgta tgccaattta ttttttgatg
     8821 ctggttatgt ccgtgacaaa cgcttctatc aaaacaaccc tttatcaaat aagtggctct
     8881 atagctttgg cgcaggactt gactttgtaa cttactacga tttggtgctt cgtggcgaat
     8941 ttgcatttac aggatatggt aaatccggaa tttttctgca ccttacagcg ccaatttaat
     9001 aactttgctc gatgacaatt gccgtatatg ccagaaatgt aaatgaaaaa gtgcttgcag
     9061 cactcgatgt tgtatggaca caattacatc agaatgatgt tgacgttttg ttgtatggaa
     9121 cattgtatcg tcacctgaaa aagtcgggga aggaaatgca aaacacacat gttttcagca
     9181 cctcagcaga gatagcaggt aaagccgatt ttcttttcag cataggtggt gatggcacca
     9241 ttctcgacac cattaaattg gtaaaagact caggcattcc tgttcttggc atcaataccg
     9301 gcagacttgg ctttttatct attgttttgc ccgaggctat aaccgaagca ataaacagta
     9361 ttctcaaagg gcattattca ctcgataaaa gagcaatgct taggttagaa accgataaag
     9421 aaatttttgg tgatgtaccc tatgctttaa acgaacttgc cattcataag aaggatagtt
     9481 catccatgat aattatccat gcttttttaa atggagaata tctgaactct tattgggcag
     9541 atggacttct gatagccaca cccacagggt caacaggtta ttctttaagt tgcggaggac
     9601 ctgttatttc accaggatca ggaaattttg tgattacacc cattgccccg cataacctta
     9661 atgtgcgtcc tatcgtagta tcggataaaa atgtagtttc tttggaagtt gaaggtcgaa
     9721 acccatattt tatggcatca ttggactcca ggtcagtaac catagattca tccgtgcaac
     9781 tggctgtcag acgggaggat ttcagtttca atctggtaag gttcgataac gataattttc
     9841 ttcaaaccct aagaaataag ctaaattggg gccttgatgc ccgtaattgg taagaatata
     9901 ttataattgt acctgcaaat atttgaaatg tttatttttg tcaataacca ccccggctta
     9961 tgaacgataa atacaagaaa ttactgcttg ttggcttaat tttaattccc tttttaggta
    10021 gtgcacagat tgctaaaaaa cgctggaagg cgtaccgtat tgagtatatt gcaggactcg
    10081 gagcaaccaa ttttttaggt gaacttggag gcgcaaacag agtaggctca catgctttcc
    10141 gcgatttgga attttcgatg acgcgccctg ccgctatgat tggctatcgc tacaaacttt
    10201 caccaacttt tgcatggtgc tctaagtttt cgtatggaat agtttcaggt gatgatgctt
    10261 tgacaaaaga gcatttcaga aataaccgta acctcagttt caaatcaaac atctttgaat
    10321 taagcaccaa tattgaaatt tcttttcttc aagaacaagt tggtcaccgc tataaacttc
    10381 gtggcgttag aggtttgcga aatgttgaaa tctctgccta tggattttta ggcattggcg
    10441 tatttcactt taatccaaaa gctaaacttg atgggcagtg gtatcagctt cagcctttgg
    10501 gaacagaagg tcagggacaa gttgcatcta gaagtaaata taaacgtaca caattatgtg
    10561 ttcctttagg acttggtgca aaatatgcaa ttgaccgtca gataagcgta gggcttgaat
    10621 taggtttacg ctacacgttt actgattata ttgatgatgt aagtaaaagc tattattacg
    10681 acagaggcgg ctcattagtt gaaatccttg cagaccgcag ccatgatgat aactcacatg
    10741 taggtgagca gcgtggcgac ccaagatgga atgatgccta tgtttttgca atgtttaata
    10801 tttgttataa aataagaacg ggtagagtaa attatcctat gttctgatgt aattttcaga
    10861 ataattttcc gtttttattg ctgtttttca gccatgcgca aaattttaat cttatttttt
    10921 acaatcgtgg ttcttcaacc acaaaaaata tcagcacaaa gtagtgagtt aggattactg
    10981 cttggtgtag cttcttataa aggcgaaata aatacccatc tttttaatcc aaatgcattt
    11041 aatcctgcag caggtttata ttataaacgt tgtctcaata gtttttggtc ttttcgttta
    11101 ggacttactt atgggcagat aacaggtagc gatgccattg cagacgaaga ttttaatcaa
    11161 tatcgcaacc tatcattcag aagtgatatt tgggatgcca gcgctttgtt tgagtttaat
    11221 ttttttcctt ttcagacagc ttcggaacat acctctaaag ttgctccata tcttgttgca
    11281 ggaataacct tctttcatca caaccctcaa ggttttatta atggatcatg gtacgatttg
    11341 caacctctcg gcacagaagg gcagggtagc ggacttccaa atacaacagg aaaatataag
    11401 cgtgtgcagt ttgcttttcc tatgggtggc ggaataaaat ttaaattatc aaacaggttc
    11461 tctttatctg tagaagctat tgcacgaaga acctataccg attacttaga tgatgtgagt
    11521 acggtttatc ctgatcaggc agagttaaga aataccaatg gtgtacttgc agcagaactc
    11581 tccgacagaa caatttttgc aggcacagat attaatgcaa aacgtcaaag aggaaatgcc
    11641 tcagataaag actggtacat gatgggtggc ttcaccatca actggacact ttcaaagaaa
    11701 tataccgata agtgtaagcc attccgaact aaattgagat aaaattttta agcgttcaat
    11761 ttaaaattaa gctcaaactt ttagttaatt ttgcattccg ttttttttac aaatggattt
    11821 acttaaagat ttaaaagaaa aatatatagc acaccctgct gtacaggaac tctttttgca
    11881 aataagtaag caagcttcaa aaacacatct tcaaaatact gccggttctg cctcatcatt
    11941 tattgcaact gcaacatttg agcatgaacc tgctcccatg ctttttattt tacccgacag
    12001 agaagaggct gcttattttc ttaacgattt agaaaacctt aaccctgagc agaaagtttt
    12061 tttctttcct tcatcctaca gaaaaccttt taaactcaat gcacatgaaa atgcatctat
    12121 tcaaatgcgt acggaggtgc ttagtcacat taataaacac agtcagtcat ttattgttgt
    12181 atcgtatgcc gaagctgttg cagaaaatgt tgtaacacga catgcactcg aaaaaaatac
    12241 tattgaagcg cgtgtgaatg agcaactgag cattgatttt gtaaatgatt ttctgacaga
    12301 gaataatttt caaagaactg attttgtttt tgaacccggg cagtttgcca tcagaggcgg
    12361 tattgttgat gtttattctt atgcctatga actgcctttc agattagagt ttgatggaaa
    12421 taaaattgaa tctattcgct cattcgatcc ggtgacacaa ttgtctgaaa aaaatctgca
    12481 ctttgttacc atcattccaa acattcagac agaaatgcag cagaatgtga cagaatcgtt
    12541 ttttaaattt cttcctgaaa atactttaat ctggacacgc gatctttctt attcattaaa
    12601 ctatcttgat gataatttaa cagtgcttaa aaatcagact tctgagaatg atgaggaaga
    12661 aaagagtgat aatccaagaa ttgtttatga ttctatcgag gagattaaat ctgttctgtc
    12721 aactttcaga caggtagagt ttggtttgaa atctttttat gaaaatgtag tcggaataaa
    12781 attcaatata cagccacaac cacagttcaa taaaaacttc aatttacttg ccggtcattt
    12841 aaaagaaaat agtgataagc attttaagtc atacattttt tctgattctg ccaaacaggt
    12901 tgaacgcatc tatgccattt ttgatgattt aagttttaaa aaagaagttc agcgtgacga
    12961 tatacaattt agccctgttt atacttcatt gtacaaaggt tttactgatt tagatcttaa
    13021 agtttctgta ttcacggatc atcagatttt tgatcgctat catcgtttta aactacgtaa
    13081 gaattacagc cgaaacgaag ccatttccat taaagaattg tacagcctca agcctggtga
    13141 ttatgtaaca catattgatc atggtgtagg tcgttttggt ggattggaaa aactaaatat
    13201 agaaggtcgt cagcaagagg caatcagatt ggtgtataaa gacaacgata ttctttatgt
    13261 aagtattcac agcctgcatc gcatagcccg ctacacaggt aaagaaggca ctgcaccatc
    13321 gctcaataag ttgggtagca catcatgggc atcgctcaag caaaaaacaa aaaagaaggt
    13381 aaaagatatt gcgcgcgatc tgatagcact atatgccaag cgaaaagcac aggaaggctt
    13441 tgcatacact cccgataatt atctgcaaac agaattagag gcaagtttta tctatgaaga
    13501 tacgcccgat cagataaaag caacacgtga tgtaaaaaag gatatggaaa gcgcatcgcc
    13561 aatggacaga ttaatttgtg gtgatgttgg atttggaaaa acagagatag ccattcgtgc
    13621 agcttttaaa gccgtaaacg atagtaaaca ggttgccatt ttagtgccta ctaccatatt
    13681 ggcaatgcag cattacagaa ccattagcga aaggttgaaa gattttccct gtactattga
    13741 ttacatcaac cgttttaagt caactgcaca acaaaatgaa acactacgta aactcgaagt
    13801 aggtaagatt gacatcatca ttggcacgca caggctgctg agcaaggaca taaaattcag
    13861 taatcttggg ttgttgatta ttgacgaaga gcagaaattt ggtgttgcaa gtaaagagaa
    13921 aattaaatct cttaaagtaa atgttgacac actgactttg acagcaacac ctattccaag
    13981 aacacttcag ttttcattaa tgggtgctcg tgatttatca atcataaaca caccgccacc
    14041 aaaccgctat cctgtaacaa cagagttgca tacatttaat gacaaagtta ttcgtgaggc
    14101 aataatgtat gaagtatcgc gtggaggtca ggtgtttttt gtgcacaatc gtgttcagga
    14161 tattcacgat atttctgata tgataagaaa actttgtcct gatgtaaaaa ttgttgtagg
    14221 gcacggacag atggatggca gtcagcttga agaagtgctt atgactttta ttgaaggtga
    14281 tgcagatgtt ttggtggcaa caaccatcat cgaatccgga attgatatca gcaatgcaaa
    14341 cacaattatc atcaatcagg cacagaattt tggattgagc gatttgcatc agatgcgtgg
    14401 cagggtagga cgaagtaata aaaaagcttt ttgttatctg cttgcaccac cactcactgt
    14461 gttaacacca gaggcacgtc agcgactgca ggccatagaa gaatattccg atttaggaat
    14521 gggatttcag gtagcgttgc gcgacttaga tattcgtggt gcaggaaatt tactgggtgg
    14581 cgaacagagt ggttttattt ctgaaatagg atttgaaatg tatcagaaaa ttttagacga
    14641 agcgttgata gaattgcgcg agtctgattt taaagaatta tttcctgctg atgataacaa
    14701 accaattgtt gccgattgcc agattgaaac tgaccttgaa attttgattc ccacacaata
    14761 tgtagaaaat attgtggagc gattgaatct ttacaaagag ttagatgata tagaaacaga
    14821 agaaaaactg aatgcttttg aaagtcagct tcgtgacaga tttggccctg taccaaagca
    14881 agtaatggag ttgtttgaaa ctattaaact gcgatggaaa gcaaaatcat taggttttga
    14941 aaaactaatt ttaaagaatg ggcagcttaa aggatattta gtttcaaatc ccgattcacg
    15001 attgtataag agtgatttat ttatgcagat aatcaatttt gttaaaagca atccatcgcg
    15061 ctgccgcatg aaagaagata aaagtaaact tagtcttacc ttttacaaga tagaaactgt
    15121 gaatgaagct aatgctgttt tccggcagat gatgaaacca tcagtggcaa cggcataggt
    15181 tgattattgt acatttgcag ccttgtatgg ttctgtagta taatggatag tacgcaggtc
    15241 tccggaacct gagattcgag ttcgactccc gacaggacca cttagatttc atattgatat
    15301 taaagaagta tcaatagtaa ccgtttttct attggattgc taaaagcaaa ataatattac
    15361 ttcaccatta aaaacttacc cggtctttct gggtcggcaa taaaccaggc atcttttcca
    15421 ttagcatctt ttcctaaacg agcgcccggt attggaggat gttgtccatt gccattttgc
    15481 agatgtccgt tagaatttat tttcgattgt tcgttgtttt tatcagaagg aatgaatccg
    15541 atttcgcaaa gctcagataa actttctttg aatttagaaa caacaaagtc aacatctgca
    15601 tctgtaaaag caagtgttaa aaagcatgga aagccatcca tgatgtgaac acctttatag
    15661 cgcatcattg caaaaagtaa ttcagtgtaa tgaggctctg tttcccattt gcctttaaat
    15721 aaagagccaa aggttaccaa cttaaatggt gagcctaatt ttttactgtg tgcatttact
    15781 tcgtcaacca atctttgtgt tttagcattc aacttttcat aaagcggctt gccttctttt
    15841 ttaagatgca acaacacttc tttcattgct gccatagcaa gcggatgacg tacaaatgtt
    15901 ccagcaaaat aggttacacc aacttcagga atagaattat caccatactg ccagaatcca
    15961 ccatctaaag catccatgta ttgtttctta cctgcaatcg caccaacagg catattgccg
    16021 ccaataactt ttccgtaagt actcaggtcg gcatcaacac cataatattc ctgcgcaccg
    16081 ccaagtctta atctgaatcc tgttatcacc tcatcaaaaa tcatcagcac attattttct
    16141 ttagttagtt ttctaacctc atgaataaat tctttaggat gaaaatctgc tctgcgtgat
    16201 tgaataggtt caatcattac ggcagcaatt tcatccatgc gctcgcggat aatctgcaaa
    16261 ctctcttctg tgcc
//
LOCUS       DAOOOI010000049        15177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_783_length_15177_cov_32.931160, whole genome shotgun sequence.
ACCESSION   DAOOOI010000049 DAOOOI010000000
VERSION     DAOOOI010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 15177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15177
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_783_length_15177_cov_32.931160"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PKX59_07285"
     CDS             complement(<1..596)
                     /locus_tag="PKX59_07285"
                     /inference="COORDINATES: protein motif:HMM:NF015081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOZ90707.1"
                     /translation="MITRKQPVTMMYSKALIKPDGEVLNYSDTSSGNEVLVLIHGYPF
                     DQSIWKEQVIALSDHARIITYDVRGYGSSSKGKERFSIDLFADDIIFLLDSLQVSKFI
                     VCGLSMGGYIALNLVKRYPERIKGLVLYDTQCYADNAIGKAKRYENIELIRNGGKQKY
                     LEDMCKILFSPVTFQKNNEIIKQTLDLMMKADNEVLMQT"
     gene            complement(611..2383)
                     /locus_tag="PKX59_07290"
     CDS             complement(611..2383)
                     /locus_tag="PKX59_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOZ90708.1"
                     /translation="MEVTRLIDLFPYQLSRFPKADALAGKQDGVWKKYATADIIETIN
                     NLSYGLLHSGIRPGDKIGIIANNRPEWNFVDHACLQIAVADVPLYPTISENDLKFTLN
                     QSAVRYLFVANVDLYKKVSQIKNDLPALEKIYLFDRYQDLPHWSELIDLGLKHPKQEE
                     VKQIRDSIKPDDLATIIYTSGTTGNPKGVMLSHNNLVSNFKFCDVLCPFHESWKALSF
                     LPLNHVYERMLSYLYVYFGVSIYYAESLDTIADNLKEIKPQIFVTVPRLLEKVYDKIV
                     SKGAELKGIKKALFFWALNLGLRYELKGANGWWYEFQLRLANKLIFSKWREALGGNIQ
                     AIASGGAALQPRLARVFHAAKIPVLEGYGLTETSPVIAVNNFQPDSIKFGTVGTVLEN
                     VEVKIAADGEILCKGPNVMLGYYNSPELTAEVIDADGWFHTGDIGVFEDGKFLKITDR
                     KKEIFKTSGGKYIVPQMIENKLKESRFIEQAMVIGENQKFASAFIVPNFAFIRDWAAK
                     KNISVKSNAEIAASSEVKKRIAEEVEVVNKTLGHYETIKRFELLDREFSIERNEMTPK
                     LSLRRKIILENYKDKLAKIYKGTE"
     gene            2452..2835
                     /locus_tag="PKX59_07295"
     CDS             2452..2835
                     /locus_tag="PKX59_07295"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HOZ90709.1"
                     /translation="MNNNDLLNKSKPLLIFDGNCAFCRSCIRFVKKYDIKLQFDFADN
                     NQNTTKLILAQYFINPESLNSVVLIEYNKLHIQSDAIIKTLLLLGGRWRLLARIMMVF
                     PNSFRNKVYNIIAANRNKLRSHCTV"
     gene            2832..3830
                     /locus_tag="PKX59_07300"
     CDS             2832..3830
                     /locus_tag="PKX59_07300"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HOZ90710.1"
                     /translation="MKTNFTEQTGIAYPIIMAPMFLVSNEAMMQSAIRTGIMGVFPTL
                     NYRKEGELDALIKRLHQFKSENQGSFGVNLIVQKTNPLYNRHLKICVENKVPFYITSL
                     GSPREVIEAAHSYSAKVYCDVTNIGHAEKCAKLGCDGFIAVGQGAGGHAGPYAQTVLI
                     PTLRKHFPSIPVIAAGGIATGDGIAAMMALGATGVSIGTRYIASTEAEVSDEYKNSIL
                     NSGMDNIVMTERLSGTPCNIINTPYAKKIGYKQNWLEKWLSNNSTTKKYFKMMVQYKG
                     VKALEQAIKPGNYKTLWTAGQTVELIDDIINCDEITERLIKETHLSVENLSNKFKD"
     gene            3983..5476
                     /locus_tag="PKX59_07305"
     CDS             3983..5476
                     /locus_tag="PKX59_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90711.1"
                     /translation="MAFKRIFFFVWFSVCFHVAHAQKVTYAQQELDAYSYDYIKTAGY
                     NDNSFFVFQSNLPFENDRDRVGFKSRKYKVTCYNLNLSLKWSKKIEPKNDNEKLETIT
                     VIDGQVALIKSSFEGKDNQLSVFITTLDDNGNESATKKEIGTILFNRTSNLDKIKVIA
                     SKGNNYFGLIQNEKKQNGLQQLHCLIIDSDFRLVRQLQFEVPYPEKNFNYENWLLTDD
                     ADFILEGSYYSKEKSENRKRWDAYKLFVSSNGNSGAIEYAINTSNLILSSSAIAYDAI
                     NKKIVLAAFYTDNVSTKSGIIYASLGTSGNDSLLMIRQPIGEVTLTRMFAGQNLDRSR
                     AGIENLSIEKVILRNDGGAVLVTEVSYVTDYSYYDYFTQNYIRNFEYHFNNVITFSIN
                     ADGTIDWDAVLRKDQISTNDYGKYSSFALAMTENDISLLYNTRTDRNNGVAYFGIKRN
                     GETESKEIIKPEERILLVPFAAKQISTDEIIVPCWNRKKMLMAKITF"
     gene            5476..6255
                     /locus_tag="PKX59_07310"
     CDS             5476..6255
                     /locus_tag="PKX59_07310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ90712.1"
                     /translation="MPGSLSIALQYISYYVKAWNKHSVHSPFVYKLTTQVIHPDKKLA
                     KFIPIEQQRILLKQSTDKIFFTELGAGNKSNKRSVSDIAAKSLKSPRYARLLYRLANH
                     FQPDNIIELGTSLGITTGYMALSGCKNIFTIDGNKTVSEIAQSTLTSIKLHQNIKFLQ
                     GNFDEALPALLHPLNKIDFVFFDGNHRYQPTINYFHQCLTKAHDKSVFVFDDINYSQE
                     MKQAWHEIKQHPSVIVSIDLFMMGIVFFDTDLLRQHFVIRY"
     gene            6314..7033
                     /locus_tag="PKX59_07315"
     CDS             6314..7033
                     /locus_tag="PKX59_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ90713.1"
                     /translation="MPQSIISLSDISRLYKVGTETIYALRSVSLNIFKNEYVALMGSS
                     GSGKSTLMNILGCLDTPSSGSYHLNNISVSGMTDNELAEVRNKQIGFIFQTFNLLPRS
                     TALENVALPLVYAGVSKEERLKRAKEVLESVGLADRIMHKPNELSGGQRQRVAVARAL
                     VNHPSIILADEPTGNLDSKTSEEIMLLFEEIHQRGNTIIVVTHEEDIARHAHRIVRIK
                     DGLIEDDAINQQITKAKTIVS"
     gene            7030..7740
                     /locus_tag="PKX59_07320"
     CDS             7030..7740
                     /locus_tag="PKX59_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOZ90714.1"
                     /translation="MNIKSIQSLLIVVVLLASCGVANSQKGKLLTPEVFEKMLHETPE
                     AQVIDVRTPEEFNDGHLKNALNMNVNSKDFENRAPYLDKEKPLFVYCYSGGRSAKACE
                     YFEKMGFKVIYDMEGGYSSWTDANLPVEGKKADKAGISLDNFALLTSAGKVLVDINAP
                     WCPPCVKMKPMLDSLDTAWKDKVKIVRLNKDENQLISKSLQINELPTLLFYENGKLVK
                     RESGYKNAEQLKAMTQVE"
     gene            7998..8498
                     /locus_tag="PKX59_07325"
     CDS             7998..8498
                     /locus_tag="PKX59_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ90715.1"
                     /translation="MNISALAEAEKLLFIARQSLSEGKLPQAIDMLNKIVTDYPDFSK
                     AHITLADIYFNKLQDSTTAEEYYKKAIAIADMNTDAFTGYANLMLQQEKYPEALSMLN
                     KAMAIKGAPKDTIYMLLGKLYEMQGRLDDAIGNYKKCITTTLSNSVLEEAELALKRCE
                     VKKKYI"
     gene            8650..11850
                     /locus_tag="PKX59_07330"
     CDS             8650..11850
                     /locus_tag="PKX59_07330"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOZ90716.1"
                     /translation="MKRVFTISLCFFVFYSISFAQLDQYASEQNKFYWKNRKPTAGYW
                     QQDVYYNIKAAIDEKTDIISGVEELTYRNNSPDTLKFIFMHLYQNAFVPGGYLNDLNK
                     ANQNKVRYGKYELQGLGIEITNINTLSVNGITINKQAEKTIDNTIMKIMLPQPLLPGH
                     DLVLKIDFKTYYDKTGQRRRLQMFENAGNKHYNGCQWYPKMCVYDSHRAWNTDQHLGR
                     EFYGEFGVYDVELTFANNFIVEATGVLVNEKEVLPDELRKKLDIKNFKERPASPQPST
                     IIIPDGSTKTWKFHAENVHDFAFTADPSYRLSDAYWNGVRCVAIAREQHCYGWQSAAE
                     YTARIIEVFSRDFGMYDYPKIVTADANDGMEYPMITMDNGTDPGFRGLLCHEIGHNWF
                     YGMVGSNETYRPLLDEGFAQLLKSWGMEILEGYHIKQRETTWKDQKRTEPLTYQMTTA
                     YGLYYNYVLNDKDGFINTHSDGFSSALGHGGGYGMVYYKTATMLYNLQYILGDSLFLK
                     AMQHYVAQWKFCHPYPEDFRNSITEYVKTNLSWFFDQWMETNKKLDYKICKYRKADEK
                     DSFNVYFKRKGRMQSSIDFSVYSNCGVKYDYHIPNNWFVKQTDATVLPKWEGWDNLRN
                     TYKAQIKIPCGISSIVIDTTDRLGDAYRLDNRLPRPFKLVFDKWQKPAYSDWKSYVFY
                     LRPDVWFNNYDGLKVGVHAESNYMNTMHFFSGDVWINTTVGQNSLSETADINKNDPAS
                     FRINYKTPLKFVAPGVNFWIGGGLLDGLTFAKSGGDLTFLNNRVRLFAEYKLMYRRNT
                     NTLEYLLYPNEWQSALMNTSVTLGAEAQYRKNKWNGSVVIQNTSASLFSDYSYSKLSL
                     TNITNYETGKIDWRLRVFGQYGYGQNVPMESALYLAGANPEELMESAWTRSRAFVPYS
                     WLGYGDKTNHFQQGGGLNLRGYAGYLVPQNIDGEQRYFYRAISGSSVSLEIDFDRLIP
                     LRPSFTKKWLKADLYFFGDAGAIATNNSNKSIQFTNLKSDAGIGTAFTIKKFGRLNAI
                     KPLTIRCDFPFLLSSAPYAEKDYVKLRYVIGIGRTF"
     gene            complement(11861..12352)
                     /locus_tag="PKX59_07335"
     CDS             complement(11861..12352)
                     /locus_tag="PKX59_07335"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOZ90717.1"
                     /translation="MLTELYTSYILTPLGYMNAITDTDSVLALEFDDKYTSGEILNGA
                     VSIQIANELQEYFSGKRKLFTLNLKPQGTVFQLQVWEKLLSIPFGKTISYNEQAVQLG
                     SAKKIRASASANGKNPIAIIVPCHRVIGTSGALTGYAGGLWRKEKLLQLESGSQQIEL
                     FSQ"
     gene            complement(12389..13543)
                     /locus_tag="PKX59_07340"
     CDS             complement(12389..13543)
                     /locus_tag="PKX59_07340"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HOZ90718.1"
                     /translation="MKNKFWLAIAALAATTIVSCDKVDGPYKEENTTNIGTLPGTLND
                     IRVLNATTTADDVDVLVAKFSLESSSPKTIAVQFTWHGAQSNVISEVATGNDAIFDND
                     SVYITAPAPFGTLTFKYSATISDTLTGQADFVVPYTPANPVKKVLLEDYTGRKCGNCP
                     RAQRIIANTLEPLYHEQLVVATNHAGVYAVPDNPPSCFSEDFRNSNSDELNTFFGLSA
                     YPSSMINRVGYPTTHIKYYNSWQSVISQELSKPNDAFINIYSAYSESNRTAQLTVSTE
                     FLNNLGDDYKLAVFLVEDSIHGCQLDYDLTTPTQIDSAYIHRHVLRAALNSSFGESLI
                     NDPDLTTKYIRLYNYQIPDKYNVDQCYILAYVYQTNNYYVLQSEIRKVKP"
     gene            complement(13564..>15177)
                     /locus_tag="PKX59_07345"
     CDS             complement(13564..>15177)
                     /locus_tag="PKX59_07345"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HOZ90719.1"
                     /translation="RILPVIFLGAPTLSLAQSSPGEIHGSFQIDGQYYIQDSTISAPE
                     VAEKYLLNGYGDLVFTKGNFKAGVRYETYQNVLLGYDKRFNGSGIAHRYAEYDNGELA
                     ITVGNFYEQFGSGLILRSYWEPTLGYDNALDGVRVKFKPYSGILIKGIIGKQRYYFKT
                     GEGIVRGVDAEVGINDLIKKWNDKKTRVTAGFGFVSKYQKDDDNVYILPENIGAYAGR
                     LDVTRGKVTINAEYAYKYNDPSSLNNYSYKFGDAALLKATYSQKGLGILLAAKRIDNM
                     NFRSERNANLNDLLINYLPALTKYHTYSLASIYPYATQPNGEFGFEAEVNYTIKKGTK
                     LGGDYGTNIIVNYSGANSLSTEPVAGKDLYKSDWGKFGKDVYYKDFSLEITRKMSKNL
                     KITLMYINQVYDKDKIQFQGSKQYGTIYDNIIVADITFQLNDKNAIRTEWQTLQSEQD
                     YKSWIAGLIEYSHSPNWFVAVSDQYNYGNEKSDKRIHYFNCFVGYTKDSHRITLGYGR
                     QREGLFCVGGVCRQVPSSNGLTVSISTSF"
BASE COUNT         4967 a         2788 c         2640 g         4782 t
ORIGIN      
        1 gtttgcatca acacttcatt atcagccttc atcataaggt ctaatgtttg ttttattatt
       61 tcattgtttt tttgaaatgt gacaggtgaa aacaaaatct tacacatatc ttcaagatat
      121 ttttgctttc caccatttct gattaattca atattctcat aacgttttgc tttccctatt
      181 gcattatcgg catagcattg tgtatcgtat aaaacaagtc cttttattct ttcaggatat
      241 cgtttgacta aatttagtgc aatgtatcca cccatagata agccacagac aataaactta
      301 ctcacttgta atgaatcaag caaaaaaatt atatcatcgg caaataaatc aatagaaaat
      361 ctttctttcc cttttgagct gcttccatat cctctaacgt cataggtaat tatgcgagca
      421 tgatctgata aagcaattac ctgctctttc cagattgact gatcaaatgg atatccatgg
      481 attaacacca gcacctcatt accggaagat gtatcggaat aatttaatac ttctccgtca
      541 ggctttatta atgcctttga atacatcatt gtaacaggtt gttttcttgt tatcatcagg
      601 aaaaattact tcattcagtg cctttgtaaa ttttggccaa cttatcttta taattttcga
      661 gaataatttt tctgcgcaag cttagctttg gtgtcatttc attgcgctct atactgaatt
      721 cacggtcgag taactcaaat cgtttaatcg tttcataatg acctagcgtt ttattcacta
      781 cttctacttc ttctgcaatg cgtttcttta cttcactact ggccgcaatc tctgcattgg
      841 attttactga aatatttttc tttgctgccc agtctctgat aaatgcaaaa ttcggaacaa
      901 taaatgctga tgcaaatttt tgattttcgc caattaccat tgcctgttca ataaatcttg
      961 attcctttaa tttattttct atcatctgtg gcacgatata ttttcctcct gatgttttaa
     1021 agatttcttt cttgcgatct gtaattttca aaaactttcc atcttcaaaa acgccaatat
     1081 caccggtatg gaaccagcca tcagcgtcaa ttacctctgc tgttaactct gggctgttgt
     1141 aatatccaag catcacatta gggcctttgc acaatatctc accatctgca gcaattttta
     1201 cttcaacatt ttcgagtaca gttcctacag tgccgaattt aatgctgtcg ggctgaaaat
     1261 tatttactgc tataacagga cttgtttcag tcagtccgta accttcaagc acaggaattt
     1321 ttgcagcatg gaatactcgt gccaagcgtg gttgcagtgc tgcaccaccc gaagctattg
     1381 cctgaatatt tccacccaat gcctcacgcc atttactgaa aataagctta tttgccaagc
     1441 gcaactgaaa ctcataccac catccattag cacctttcaa ttcataacgc aaacccagat
     1501 tcaatgccca gaaaaacaaa gcttttttta taccttttaa ctctgctccc tttgaaacta
     1561 ttttatcata aactttctca agcaatcgcg gaaccgttac aaaaatctga ggtttaattt
     1621 cttttaaatt gtcggcaata gtatctaaac tttcagcata ataaattgaa acaccgaaat
     1681 aaacatagag ataagaaagc attctttcat agacatggtt aagtggtaaa aaacttaatg
     1741 ccttccatga ctcatgaaac gggcacagca catcacaaaa tttaaagttg cttacaagat
     1801 tattatgcga caacattaca ccctttggat ttcctgtagt tccggaagtg taaataatcg
     1861 ttgccaaatc atcaggcttt atactatctc ttatctgctt aacctcttct tgttttggat
     1921 gcttaaggcc aagatcaatc aattcgctcc aatgtggtaa atcctgatag cgatcgaata
     1981 aatatatctt ttctaacgca ggaagatcgt tcttaatttg actaaccttc ttatataaat
     2041 ccacattagc aacaaataaa tagcgcactg ctgactggtt caacgtgaat tttaaatcat
     2101 tttcactaat agtaggataa agcggaacat cagcaactgc aatctgaagg caagcatgat
     2161 caacaaaatt ccattccggt ctgttattgg caataattcc gattttatct ccgggcctta
     2221 ttcctgaatg taataatccg taactcagat tatttattgt ttcaattata tcggctgttg
     2281 catatttttt ccatactcca tcctgctttc ctgccaaagc atctgcttta ggaaaacgtg
     2341 aaagctgata tggaaacaag tctataagtc gtgttacttc cattcttcaa ttagttttgt
     2401 tgtgctgcat aaaaatataa aaagtattta aatgtttttt actattgcaa tatgaacaat
     2461 aatgatttat taaacaaatc aaagccatta ttgatatttg atggcaattg tgctttttgc
     2521 agatcatgta ttcggttcgt taaaaaatat gacatcaaac ttcaatttga ctttgcagac
     2581 aacaatcaga ataccaccaa acttatttta gctcaatact ttataaatcc tgaatcttta
     2641 aactccgttg ttttgataga atacaacaaa ctacacatac agagtgatgc aattataaaa
     2701 accttacttc ttcttggtgg gcgttggagg cttttagccc gaataatgat ggttttccca
     2761 aactccttca gaaataaagt ttacaatata attgcagcca atagaaataa attaagaagt
     2821 cattgcacag tatgaaaacc aattttacgg agcaaacagg catagcctat cctattataa
     2881 tggcacccat gtttttagta agcaatgagg ctatgatgca atctgcaata cgaaccggca
     2941 ttatgggtgt gtttccaact ttaaactaca gaaaagaagg tgaacttgat gcattaatta
     3001 agcggttaca tcaattcaag tctgaaaatc aagggagttt cggtgtcaat ttaattgtac
     3061 aaaaaaccaa tcctctctac aacaggcatc taaaaatatg tgttgaaaac aaagtgcctt
     3121 tttacattac ttcgttaggg agcccacgcg aagttattga agcagcacat tcttatagcg
     3181 caaaagttta ttgtgatgta accaatattg gacatgcaga gaaatgcgct aaacttggtt
     3241 gtgatggctt tattgccgtt ggtcagggtg caggcggtca tgcaggccct tatgctcaaa
     3301 cggtattgat tccaacttta cgaaaacact ttccttctat acctgtaatt gcagcaggag
     3361 gtattgccac cggtgacggc attgctgcaa tgatggcttt aggtgctaca ggagtaagta
     3421 taggaacaag atatattgca tctacagaag cagaagtcag tgatgaatat aaaaacagta
     3481 ttttaaattc aggtatggat aatattgtta tgacagaaag gctttccgga acaccatgca
     3541 acatcatcaa tacaccttat gcaaaaaaaa ttggctataa acaaaattgg ttagaaaagt
     3601 ggctgagcaa taactccaca acaaaaaaat attttaaaat gatggtgcag tacaaaggcg
     3661 ttaaagcact tgaacaagca attaaaccag gtaattacaa aacattatgg actgcagggc
     3721 aaactgttga acttattgat gatataataa actgtgatga aattacagag cggttaatta
     3781 aagaaaccca tctgtcggtc gaaaacctta gtaataaatt taaggactaa acctatcctt
     3841 ctttcttggg gaaaaggtga ttgaggtaaa atgtcatttt taaagcctca ttacattcaa
     3901 tttcatgctt cgttttcatt ttagtttcag caataatata tctgtttttc gttgaattag
     3961 cctacatttg aacaaaaaat aaatggcctt taaaagaata ttcttttttg tttggttttc
     4021 agtttgcttc catgttgcac atgcacaaaa agttacttat gcacaacagg aacttgatgc
     4081 ctattcttat gattatataa aaacagcagg ctataacgat aattcctttt ttgtttttca
     4141 aagcaatctg ccatttgaaa acgatcgtga cagagtagga ttcaaatcac gtaaatataa
     4201 agttacttgt tacaatctga atctatcttt aaaatggtcg aaaaaaatag aacctaaaaa
     4261 tgataatgag aaattagaaa ctataactgt gattgacggt caggttgctt taattaaatc
     4321 ttcttttgaa ggtaaggaca atcaattgtc agtgttcatt actacattag atgacaatgg
     4381 aaatgaatct gctaccaaga aagaaattgg aactatttta tttaacagaa caagcaactt
     4441 agataagata aaagttattg cttcgaaagg gaataactac ttcggactta ttcaaaatga
     4501 aaagaaacaa aatggtttgc agcaactaca ttgcctcata attgatagtg actttaggct
     4561 tgtccggcaa ctgcaatttg aagtaccata ccctgaaaaa aatttcaact acgaaaactg
     4621 gcttttgaca gatgatgcag actttattct tgaaggaagt tactactcaa aagaaaaaag
     4681 tgaaaaccgc aaacgatggg atgcatacaa gctatttgta tcgtccaatg gaaatagtgg
     4741 cgccattgaa tatgctatca ataccagcaa cctgatatta agcagcagtg caatagctta
     4801 tgatgccata aataagaaaa tagttcttgc cgctttttat actgacaatg tttctacaaa
     4861 atccggaata atttatgcaa gtcttggaac ttcaggaaat gattcgttgt tgatgataag
     4921 acaacctatt ggcgaagtca cgttaacacg catgtttgcc ggtcaaaatt tagaccgtag
     4981 cagagcagga attgaaaacc tgagcataga aaaagtgatt ttacgaaatg atggtggtgc
     5041 tgtattagtt acagaggtgt cttatgttac agactactcc tattatgatt attttacaca
     5101 aaactacatt cgtaatttcg aatatcattt caataatgtc attacctttt ctatcaatgc
     5161 cgatggtact attgactggg atgcagtttt aagaaaagat cagatttcta caaatgacta
     5221 tggaaaatat tcttcgtttg ccttagccat gacagaaaat gatatcagtt tactctataa
     5281 cacccgcaca gacagaaata atggagttgc ctatttcgga ataaaaagaa atggcgaaac
     5341 agaatcgaaa gaaatcatca aacctgaaga acggatttta cttgtgccat ttgctgcaaa
     5401 acaaatcagc actgacgaaa ttattgtccc ttgctggaat agaaaaaaga tgctaatggc
     5461 aaaaattact ttctgatgcc gggatcatta tctattgcat tgcagtatat ttcctattac
     5521 gttaaggcat ggaacaaaca cagtgttcat tctccttttg tatataagct cacaacacag
     5581 gttattcatc ccgacaaaaa acttgcaaaa tttataccta ttgaacaaca aagaatactt
     5641 ttaaaacaaa gtacagacaa aatttttttt accgaattag gtgctggaaa taaaagcaat
     5701 aaacgaagtg tttccgacat tgccgcgaag tctttaaaat ctccgcgata tgcgcgttta
     5761 ctttataggc ttgcaaatca ttttcaacct gacaatatta ttgagttagg cacatcattg
     5821 ggcattacaa caggctatat ggcattatca ggctgtaaaa atatttttac tattgatggt
     5881 aataaaactg tatcagaaat tgcgcaatct acattaacct cgataaagct gcatcaaaac
     5941 ataaaatttc ttcaaggaaa ttttgatgaa gcacttcctg cattattaca tcctttaaat
     6001 aaaattgatt ttgttttttt tgacggcaac caccgttatc aaccaacaat taattatttt
     6061 catcaatgct taacaaaagc acatgataaa agtgtgtttg tgtttgatga tattaactac
     6121 agtcaggaaa tgaaacaagc atggcatgaa atcaaacaac atccatcagt tattgtcagt
     6181 attgacttgt ttatgatggg aattgttttt tttgatacag accttctgcg tcaacatttt
     6241 gtaatcaggt attaacattt ggtaacttaa tcataagcca caatttatta cttttaccac
     6301 gtacaaatca attatgccac agtccattat ttcgctttca gatatttcac gactttataa
     6361 agtcggaaca gaaacaattt atgcattacg atctgtttca cttaacattt ttaagaatga
     6421 atatgtggca ttgatgggta gttccggttc aggaaaatcc acgttgatga atattcttgg
     6481 ttgccttgac acgccatcaa gtggcagtta tcatctgaac aacatctctg tttctggaat
     6541 gacagataat gaacttgccg aagtaagaaa taagcaaata ggttttattt ttcaaacatt
     6601 taatctgttg ccacgaagta cagcacttga gaatgtagca ttaccgcttg tgtatgcagg
     6661 tgtttcaaaa gaagagcgtt taaagcgggc taaagaagta ttggaaagtg ttggcttggc
     6721 cgacagaatt atgcataagc caaatgagtt atccggtggt cagcgtcagc gtgtggctgt
     6781 tgcaagggca ttggtcaacc acccttcaat cattcttgct gatgagccaa caggaaatct
     6841 tgattcaaaa acctctgagg agattatgtt gctatttgaa gaaatacatc agcgtggcaa
     6901 tacaatcatt gttgtaacac atgaagaaga cattgccaga catgcacacc gtattgtcag
     6961 aataaaagac ggactcattg aagatgatgc catcaaccaa caaataacaa aagcaaaaac
     7021 aattgtatca tgaacattaa atcaattcaa tcgctgctta tcgttgtagt gctattagca
     7081 agttgtggag tagcaaacag tcagaaagga aaactcctta cgcctgaagt atttgaaaaa
     7141 atgcttcacg aaacacctga ggcacaagtt atagatgtaa gaacacctga agaatttaat
     7201 gatgggcatt tgaaaaatgc tttaaatatg aatgtcaaca gcaaagattt tgaaaaccgt
     7261 gcaccctatc tcgataaaga aaaacctctt tttgtttatt gctattccgg tggtcgcagt
     7321 gccaaagcct gcgaatattt tgagaagatg ggattcaaag tcatttatga tatggaaggc
     7381 ggctattctt catggacaga tgcaaacctg cctgtagaag gaaaaaaagc tgacaaggcc
     7441 ggtatcagtc ttgataactt tgccttgctt acaagtgccg ggaaagtttt ggttgatatt
     7501 aatgcaccat ggtgtccgcc atgtgtaaaa atgaaaccta tgttagattc attagataca
     7561 gcatggaaag acaaagtaaa aattgtgcgg ctgaataaag acgaaaatca attaatttca
     7621 aaatctcttc agattaatga attgcctacc ttactttttt atgaaaacgg taaattggtg
     7681 aaacgtgaaa gtggctataa aaatgctgaa caactaaagg ctatgactca ggttgagtag
     7741 ttgagtggat ttacttttta ttctactttt aaatgagatt gctttagaat aattcattaa
     7801 taaatcccga atattaaaaa cgccttctct gctaaatcat attgacctat ctcactgatt
     7861 cagaacacat agaattaata tttttttagt cttgccttct ccttttcaaa agtgtaaatt
     7921 gcgctttttc agacatataa aacgtgtaac ctaaagggca taactacgta taaagtcatt
     7981 tagttgttga gcaaattatg aatatatctg cattagcaga agccgaaaaa cttctattta
     8041 ttgcaaggca gtcgctttca gaagggaaac tgccccaagc aatagatatg ctcaataaaa
     8101 tcgttaccga ctatcctgat ttttcaaaag cacacatcac tctggcagat atttacttta
     8161 ataaacttca agactcaaca actgcagaag aatattataa aaaagctatt gccattgccg
     8221 atatgaatac tgacgccttt accggctacg caaacttaat gctgcaacag gaaaaatatc
     8281 ccgaagcact gtcaatgctc aacaaagcta tggccataaa aggtgctcca aaagatacta
     8341 tctacatgct gctgggcaaa ctctacgaaa tgcaaggcag actcgatgat gctattggaa
     8401 actataaaaa atgtattacc actaccctat caaactccgt tcttgaagaa gcagaacttg
     8461 cattgaaaag atgtgaggtc aagaaaaagt atatttaatt ttttctattt acaacgttgt
     8521 ttttagtgct ttgcaaccta ttctattctg ctttacaact tacagataga atttaaaacc
     8581 ccatttcagg gtaagccaaa aattttcacg aatacattac atttgccttt aaactacaac
     8641 tgtaaattaa tgaaacgtgt ctttaccatt tcactgtgtt tttttgtatt ttattctata
     8701 agttttgctc agttagacca atatgcttct gaacagaata aattttattg gaaaaatcgt
     8761 aaacctactg ctggctactg gcaacaggat gtttattata acattaaagc tgcaatagac
     8821 gaaaaaacag acattatcag tggtgtagaa gaacttactt acagaaataa ttctcccgac
     8881 actttgaaat ttatttttat gcatctctat cagaatgcat ttgttcccgg tggttacttg
     8941 aacgacttaa acaaagccaa tcaaaacaaa gtgcgttatg gaaaatatga attgcaagga
     9001 ttgggaattg aaattactaa tataaataca ctttctgtca atggtattac tataaacaaa
     9061 caagcagaaa aaacaattga caataccatc atgaaaatta tgttgccgca acctttgttg
     9121 ccgggtcatg atcttgtttt gaaaattgat tttaaaactt attacgataa aaccggtcag
     9181 cgcagacgct tacagatgtt tgaaaatgca ggcaacaaac attacaatgg atgtcaatgg
     9241 tatcctaaaa tgtgtgttta cgattcgcat cgtgcttgga acaccgatca gcatcttggc
     9301 agagaatttt atggagaatt tggtgtgtat gatgtggaac ttacttttgc aaacaacttt
     9361 atagtagagg caacaggtgt tttagtcaat gagaaagaag ttttacctga tgaactgcgc
     9421 aagaaacttg atattaaaaa ttttaaagaa agacctgctt caccacaacc ttcaacaata
     9481 atcatccctg acggaagtac gaaaacctgg aaattccatg cagaaaacgt tcatgatttt
     9541 gctttcactg ccgatccttc ctatcgtctt tctgatgctt actggaatgg tgttcgttgt
     9601 gtagctattg cccgcgagca acattgctac ggatggcaaa gtgcggcaga atatacagca
     9661 cgtattattg aagttttctc acgcgatttt ggaatgtacg attatccgaa aattgttact
     9721 gccgatgcaa atgatggaat ggaatatcca atgataacaa tggacaatgg tacagatccc
     9781 ggattccgtg gcctgctatg tcatgaaata ggacataatt ggttttatgg tatggtagga
     9841 agtaatgaaa catacaggcc attgcttgat gagggatttg cgcagttgct gaaatcgtgg
     9901 ggtatggaaa tacttgaagg ctatcatata aaacagagag aaacaacatg gaaagatcag
     9961 aaaagaacag agcctttaac ctatcagatg acaacagcat acggcctcta ttacaattat
    10021 gttctaaatg ataaagatgg atttatcaat acacatagtg acggtttcag cagtgcttta
    10081 ggtcatggtg gtggttatgg aatggtttat tacaaaacag caaccatgtt atacaacctg
    10141 caatatattt taggtgacag tttgttttta aaagcaatgc aacactatgt tgcacaatgg
    10201 aaattttgtc atccctatcc tgaagatttc agaaacagca ttacagaata tgttaaaacc
    10261 aatttaagct ggttttttga tcagtggatg gagaccaata aaaaattaga ctataaaatc
    10321 tgcaagtatc gcaaggccga tgaaaaagac tcttttaatg tttattttaa gcgcaaaggg
    10381 cgcatgcaat catcaattga tttttctgtc tattcaaact gtggtgtaaa atacgattat
    10441 catattccca ataactggtt tgtaaaacaa accgatgcaa cggtattgcc gaaatgggaa
    10501 ggttgggata atctgagaaa tacttataag gctcaaatta aaatcccatg tggtattagc
    10561 agtattgtta tagacacgac agaccgttta ggtgatgctt ataggttgga caaccgcttg
    10621 ccgcgacctt ttaaacttgt gtttgataaa tggcagaaac ctgcctactc agactggaaa
    10681 agctatgtat tttacttacg tcctgatgtt tggttcaaca attatgacgg actaaaagtt
    10741 ggtgtacatg cagaaagtaa ttacatgaat accatgcact ttttttcagg tgatgtttgg
    10801 attaatacaa ctgtgggaca aaatagtttg agcgaaactg ccgacataaa taaaaatgac
    10861 ccagcatcat tcagaataaa ttacaaaaca ccattaaagt ttgttgcacc gggagttaat
    10921 ttttggattg gtggcggact gcttgatggt ttaacatttg caaaatcagg aggtgattta
    10981 actttcctaa acaatcgtgt aagactgttt gctgaataca aattgatgta cagaagaaac
    11041 accaacacgc ttgaatattt attatacccg aatgaatggc agtcggcttt gatgaataca
    11101 tctgtaacct taggtgcaga ggcacaatac agaaaaaata aatggaacgg ttctgttgtc
    11161 atacaaaaca cttcagcatc attatttagt gattactcct attctaagct ttcattaact
    11221 aacatcacaa actacgaaac aggaaaaatt gactggcgat taagagtttt cggtcaatat
    11281 ggatatggac aaaatgttcc tatggaaagt gcattatatc tagccggtgc taatccggag
    11341 gagttgatgg aaagtgcttg gacacgttca cgtgcatttg ttccatacag ttggttgggc
    11401 tatggagaca agaccaatca ctttcagcaa ggtggtggac ttaatttaag gggttatgca
    11461 ggttatcttg tgccgcagaa tattgatggc gaacaaagat atttttatcg tgccatcagt
    11521 ggctcttctg ttagtttaga gatagacttt gacaggttga ttcctttacg cccatcattt
    11581 actaaaaagt ggttaaaagc tgacctttat ttttttggag atgcaggtgc cattgcaaca
    11641 aacaattcta acaagtccat tcaattcact aatttgaaaa gtgatgccgg tattggaaca
    11701 gcatttacaa ttaaaaaatt tggtcgtttg aatgccataa agccacttac tatacgatgt
    11761 gattttccat tcttgttaag cagtgcccct tatgctgaaa aagattatgt caaactacga
    11821 tatgtaatcg gcatcggtcg cactttctga taacatcacg ctattgactg aaaagttcta
    11881 tttgctgact tcccgattca agttgcagca acttctcctt tcgccacaaa ccgcctgcat
    11941 aaccggttag tgcacctgaa gttccaataa ctctatgaca aggaacaatt attgcaatcg
    12001 gatttttacc attggcagat gctgatgctc ttattttttt cgcactccct aactgcactg
    12061 cctgctcgtt ataagagatg gtttttccaa agggtataga aagtaacttc tcccaaacct
    12121 gaagctgaaa tactgttccc tgtggtttta aatttaaggt gaataactta cgtttaccgg
    12181 aaaaatactc ttgtagttcg tttgcaatct gtattgacac agctccgttc aatatctcac
    12241 cggaagtgta tttgtcatca aattcaagtg caagcacact atctgtatct gtgatagcat
    12301 tcatataacc taacggtgtc aggatatacg aagtatataa ttcagttaac aataatggca
    12361 ataattgatt tttacaaatt gaaaatccct aaggctttac ctttctgatt tcagactgca
    12421 aaacataata gttgttggtc tgataaacat aggccagaat ataacactga tcaacattat
    12481 acttatcagg aatctgatag ttgtaaagtc tgatatactt tgttgtcaga tccggatcgt
    12541 ttatcaggct ttcaccaaaa gaactattta atgcagctct aagtacgtgt ctgtgtatat
    12601 atgctgaatc tatctgcgta ggtgttgtca aatcataatc gagctgacat ccgtgaatac
    12661 tgtcttcaac taaaaatact gctaacttat aatcatcgcc aaggttgttc aaaaactcag
    12721 tacttactgt aagttgagct gtgcgattac tttcactata tgcagaataa atatttataa
    12781 aagcatcatt cggcttactt aactcttgtg atataacaga ctgccaactg ttgtagtatt
    12841 taatatgtgt tgttggatac cctactctgt taatcatact tgaaggatat gcagataaac
    12901 caaaaaaagt attcaactca tcggagttgg aatttctgaa atcttcagaa aagcaacttg
    12961 gtggattatc aggaactgcg taaacaccgg catggttggt tgcaacaacc aactgttcat
    13021 ggtataatgg ctcaagtgtg ttagcaatta ttctctgtgc acgcggacag tttccacact
    13081 tacgtcctgt ataatcttcg agtaacactt tcttaacagg atttgcaggg gtgtatggaa
    13141 ccacaaaatc agcctgacct gttagcgtat cgctaatggt tgcagaatat ttaaaagtaa
    13201 gtgttccaaa aggcgcagga gctgttatgt aaactgaatc attatcaaag atggcatcat
    13261 tgcctgttgc cacttctgaa atcacgttag attgagcacc gtgccatgta aactgaactg
    13321 ctattgtttt tggtgatgat gattcgagtg aaaacttcgc tacaagcaca tctacatcgt
    13381 cagctgttgt agttgcatta agcactctga tgtcattaag tgttcctgga agtgtaccaa
    13441 tgttagttgt gttttcttct ttatatggac catcaacttt atcgcatgaa acaattgttg
    13501 ttgcagctaa agctgcaatt gctaaccaaa atttattttt cattttcgtg aacttttaaa
    13561 taattaaaaa cttgtactta ttgaaactgt aagtccatta cttgacggaa cctgtctgca
    13621 cacaccacca acgcaaaaca aaccttcgcg ctgacgacca tagcccaaag taatacgatg
    13681 cgaatctttt gtgtatccga caaaacaatt gaagtaatgg attcttttgt cagatttttc
    13741 attgccataa ttatactgat ctgatactgc aacaaaccaa ttgggtgagt gtgaatactc
    13801 aatcagtccg gcaatccaag acttataatc ttgttccgat tgtaatgttt gccactctgt
    13861 tctgatggcg tttttatcat ttaactgaaa ggtaatatca gctactatta tattgtcata
    13921 aatagttcca tattgctttg atccctgaaa ctgaattttg tctttatcat aaacctgatt
    13981 aatatacatt aaagtaattt tcagattttt agacatctta cgagtaatct ctaagctaaa
    14041 atctttgtag taaacatcct taccaaattt tccccaatca cttttataaa ggtctttgcc
    14101 tgcaacaggc tctgtgctca atgaattggc tccggaatag ttaacaatga tatttgttcc
    14161 ataatcacca cctaattttg ttcctttctt aatagtatag tttacttcag cttcaaatcc
    14221 aaactcacca ttcggttgtg ttgcataagg atagattgat gctaatgaat acgtgtgata
    14281 ttttgtcaat gcaggaagat aattaatcaa taagtcattt agatttgcat tacgctcact
    14341 acggaaattc atgttgtcaa ttcttttggc ggcaagtaat attcccaaac ctttttgaga
    14401 ataagttgct ttcaaaagtg cggcatcacc aaacttatag ctgtaattat ttaatgatga
    14461 agggtcgttg tatttataag catactctgc attgatggtt actttacctc ttgtaacgtc
    14521 aagtcgtccg gcataagcac caatgttctc aggcaagata tatacattgt catcatcttt
    14581 ctgatactta ctcacaaatc caaaacctgc agtaaccctg gtctttttat cattccattt
    14641 ttttattaaa tcatttatac ccacttctgc atctactcct ctgacaatgc cttctccggt
    14701 tttaaaataa tagcgttgtt taccaattat acctttaata aggattccac tgtaaggttt
    14761 aaattttaca cgaacaccat ccaatgcatt atcataacct agtgttggct cccaatagga
    14821 acgcaaaatc aatccactgc caaactgctc ataaaaattt ccgacagtaa tagctaattc
    14881 gccattgtca tattctgcat aacgatgcgc aataccactg ccattaaatc ttttgtcgta
    14941 gccaagcaat acattctgat aagtttcgta acgaacacct gctttgaaat tacctttggt
    15001 aaaaaccaaa tctccgtaac cattaagcaa atatttctct gctacttcag gtgcacttat
    15061 agttgaatcc tgaatgtaat actgtccatc aatctgaaaa ctaccatgta tttcaccggg
    15121 agaactttgt gcaagcgata aagtaggtgc acctaaaaaa ataaccggca atattct
//
LOCUS       DAOOOI010000050        14931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_797_length_14931_cov_30.784082, whole genome shotgun sequence.
ACCESSION   DAOOOI010000050 DAOOOI010000000
VERSION     DAOOOI010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14931
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_797_length_14931_cov_30.784082"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2008
                     /locus_tag="PKX59_07350"
     CDS             <1..2008
                     /locus_tag="PKX59_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ90720.1"
                     /translation="IKIIMKKLSLLLLSIACIFSVTTMDAAKAKKSKTAKADKAFNAG
                     KYAPAADLYKKAYGKQKSKLVKAEIAYKAAECYSKMSNSKEALNWYEKSAKDNGKNPD
                     AVLKYAQALKTNGRYDEAVAQFNAFKALEPGDNRGQDGATSSETSQQWKDKPTKYKVE
                     NVAALNTKYFDFAVAVSNLEKNTLYFTSSRAEASGNKNDAWYGEKFYDIFKSAQDNNG
                     KWSIPTPISEAINTAASEGAMTFDAKGNVMYFTRCQKSDEKGKEGQCKIYKSVYDGAK
                     WGTPELLPFNSDNYTCGQPSITEDGTTLYFASDMPGGAGGKDIWMTKNENGAWGTPVN
                     ISSINTVGDEMFPYIAPNGKLYFSSNGHMGMGGLDIFSATSENGAWANVTNLKYPINS
                     SWDDFGFMFNSATTGYLTSNRDGGQGQDDIYSFVVPPANFAVYGRVYDTDTKEAIAGA
                     TVELFGSDGTQLSVKTQDAGTYRYELKPDVDYKVSASFTGYLTQFKELTTKGLDESKD
                     FEQNFDFPLKSTSKPIALPEIFYDLDKYSLRPESKAALDGLIKVLDDNPTITIKLTAH
                     TDFRADDNYNLALSKKRALAAYNYLVSKKVDKDRLSWEGRGEKDPKEVENDEQYLPFK
                     RGDKLTEEFIKGLTSEELKEKAHQYNRRTEFRVLSTDYTPKKAK"
     gene            2173..3360
                     /locus_tag="PKX59_07355"
     CDS             2173..3360
                     /locus_tag="PKX59_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOZ90721.1"
                     /translation="MKDQNKYSQRGVSADKEDVHAAIKNVDKGLFPKAFCKIIPDYIS
                     NDKDYCLLMHADGAGTKSSLAYAYWKETGDISVWKGIAQDALIMNIDDLLCAGATDNI
                     LLSSTIGRNKNLISGDVIAAIINGTEHLVAELKNYGVNITTTGGETADVGDLVRTIIV
                     DSTVMCRMKRSEVVDNANIVAGDVIVGFASFGKATYETEYNSGMGSNGLTSARHDMFN
                     KSVGIKYPETFDNSLPEDVVYNGKYALTDNIILDDGYGKIEMHAAKAVLSPTRTYAPL
                     VKKIFESYRKNIHGMIHCSGGGQTKVLHFVDNLHIIKDNLFPTPALFRHIQQAAGTNW
                     IEMYKVFNMGHRLEVYCPVAIADELISIAAEFKIGAKVVGRVENCAGKKLTINSPYGK
                     FEY"
     gene            complement(3357..3722)
                     /locus_tag="PKX59_07360"
     CDS             complement(3357..3722)
                     /locus_tag="PKX59_07360"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOZ90722.1"
                     /translation="MQVKKYFFLISILTLSIVSCTHKNKEVIIPEGTLSKDSMIQILA
                     DIHLAESEATLHPYNDSLGIINLPAYYKYIFEHHRIDTAVFSHSMNFYLANPELMNTV
                     YSGVIDELSKRHARYQKNN"
     gene            complement(3726..5063)
                     /locus_tag="PKX59_07365"
     CDS             complement(3726..5063)
                     /locus_tag="PKX59_07365"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOZ90723.1"
                     /translation="MQTIIHNVRIVNDNKIFDSDIYFKNGIIERIDPSINVKHKVDEI
                     KGDGLILLPGIIDDQVHFREPGLTHKGDIASESAAAVAGGITSFMDMPNTVPNTLTQE
                     LLEEKYRLAQKKSWANYSFYMGAGNNNLDEVLKTNRENVCGVKIFMGSSTGDMLVDNP
                     TTLDKIFSQSPMLIATHCEDEQTIRQNTEKYKSLYGDKLNASHHPLIRSEEACYLSSS
                     FAVNLAKKYNTRLHILHISTARETELFSNNVPLKDKRITSEACIHHLWFDDNDYASKG
                     NFIKWNPAIKTQVDKEAILNALLSDKIDVIATDHAPHTLDEKQMPYLKAPSGGPLVQH
                     ALVAMLELFHQGKISLEQIANKMCHSPAICFNIKNRGFIQEGFAADVSLVNLNEPWTV
                     AKSNILYKCGWSPFEGTTFQSKVKHTFVNGVHIYNDGHLQLSTNGQRLLFSSH"
     gene            5217..5290
                     /locus_tag="PKX59_07370"
     tRNA            5217..5290
                     /locus_tag="PKX59_07370"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5251..5253,aa:Pro,seq:ggg)
     gene            5421..7097
                     /locus_tag="PKX59_07375"
     CDS             5421..7097
                     /locus_tag="PKX59_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOZ90724.1"
                     /translation="MALSRIWSYMILFAVAVAGYRYMQGDKNIFNDMVVEKKLAAGES
                     FSKVSYYVIGQGIDSVLKEPKLKNANWVKTDKFSDAQVIITTADLKNNLREQLNNQLA
                     KISFANINTQNKFTADKSKLIYSEELITDSTLNITNNLLQANVVLTSEVNTDSLRNAW
                     TAISNRQKIVGSNEVFLWLPKNIDGIFETGKFAVTIAIGLIGTLALFMGLLNIAERAG
                     GINIISRLISPFLSKLFPSLPKNHPSFGHMVMNFSANMLGLDNAATPFGLKAMQSLQE
                     INTNKDRASDAQIMFLALHASGLTLIPVSIIAVRSANGSHNPNEIFIPLMLTTFVCTL
                     TAMIVTSLKQKINLFQRGLLLPVAGLSLTLSLFVWFLSSLRPFALDQFSDAFSNGAIL
                     LVFFGIIAGGLYKKINLFDAFVDGAKGGFETAVRIIPYMVGMLVAVSMLRTSGAFDLI
                     INPVKHLIAISGMDTRWAEGLPTALLRPFSAGGARGFMIDAMRTFGPDSFIGKLVSVL
                     QGSSETTFYVVALYFGSVSVKDTRYAIPVMLLADLAGVIFSIVICYLLWA"
     gene            7237..9429
                     /locus_tag="PKX59_07380"
                     /pseudo
     CDS             7237..9429
                     /locus_tag="PKX59_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030837.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
     gene            9457..9972
                     /locus_tag="PKX59_07385"
     CDS             9457..9972
                     /locus_tag="PKX59_07385"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOZ90725.1"
                     /translation="MTGTISLDVSHSWMQFGISHFLTTANGTLAVDTGYINITNDLAT
                     SSIFVQLNVKSIDTKNTKRNNHLLSSDFFDAEKFPSIVFKSNKIEKAAEGSAFRYIAK
                     GELTIKNITKPVELRFNYMGALPNEYAAKDAHTFVGEADLDRIDFGVGESGPTVGNKV
                     KATFSVEGYKI"
     gene            complement(10052..10765)
                     /locus_tag="PKX59_07390"
     CDS             complement(10052..10765)
                     /locus_tag="PKX59_07390"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOZ90726.1"
                     /translation="MIVDLLIPCYMDQVFPETANNVVKIIEKLGCGVNYNVEQTCCGR
                     TAFEEGYQKECKAVGEKLIKEFQNERYIVCPGAYCSGMIKNIYPQMFHNSALHNEYKS
                     VQKNIYEFTDFLVNILNVTDVGARFNGKVVILDSCKAINELTVKDSVRTLLSKVKGLE
                     WIESTDSCCGYSPSFTEKYEAISVDIGKRKILELTQTGANTVISTDYTCLMHLKSIAD
                     NEGSSLQFMHIADMLASGW"
     gene            complement(10795..12084)
                     /locus_tag="PKX59_07395"
     CDS             complement(10795..12084)
                     /locus_tag="PKX59_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ90727.1"
                     /translation="MEKNNPKVLRAWTFYDWANSVYSLSITTAIFPIYYNSITSANGS
                     DQVTFLGRTFTNTALYSYALSFSFLVIAFLSPLLSGIADYKGNKKFFMRLFCFLGSTS
                     CASLYFFKDLANLDIGIISFILASIGWAGSIVFYNAFLPEIATADMQDKVSAQGFALG
                     YIGSSLLLIFNLAMILNPSWFGITNSGMASRISFLMTGVWWFGFSLFTFKYLPEKTST
                     HNTNEQLLTKGFNELIKVLKQLQSLPMLKRFLISFFFYNMGVQTVMYVASLFGTKELH
                     MRDDELIITVLIIQFVAIAGAYLFAFLSSKAGNIKALLIAVTIWIGICWAAYFVATSV
                     QFFVLAFAVGMIMGGIQSLSRSTYSKMLPKTQDHASYFSFYDVCDKIGLVLGTVAYGY
                     IEELTGSMRNSILVLMTFFVVGLFSLLSIRNFRLQNT"
     gene            complement(12101..12901)
                     /locus_tag="PKX59_07400"
     CDS             complement(12101..12901)
                     /locus_tag="PKX59_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90728.1"
                     /translation="MEKTTLKKIMGFRLWHCRSTGFKKLNWLLFLSQILLPIFVASFL
                     ISGCKKKSDDSPSPFLGITVYNDFKTHPDTFIFKAESTIPCNYKWNFADGSTEVSGDS
                     VMHIFDYGFYNVVLKGETAEGKTATTIKGINASPYTKANIVSLSLNSWPVHKSDGTTW
                     DSDGSAADIYCQIWNGNTYVSTTVSTNAPQGTANFNFITPFAITQFDKSIIIKVFNYN
                     FSPETDDLMETVVIEKNLTPLMTNQLPYAGQQLFNGGQTNGALNFIWQ"
     gene            complement(12874..13275)
                     /locus_tag="PKX59_07405"
     CDS             complement(12874..13275)
                     /locus_tag="PKX59_07405"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOZ90729.1"
                     /translation="MSETITPIQIRFSDIDWMGHVNNAVYLSYIEKARIDFSERLPLN
                     INWRETGFILAQTQINYRHPALLNDNLSVRTWCTRVGTKSFDLGFEVFKTTDNNKIIL
                     ADGLTVLVCYNYRLSVSENIPEEWKKLLLKK"
     gene            complement(13278..14531)
                     /locus_tag="PKX59_07410"
     CDS             complement(13278..14531)
                     /locus_tag="PKX59_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90730.1"
                     /translation="MMNRILLLMLAVISYGQSFTQCLDPYYPRIYQTYVERDISYGSD
                     TAYNNTVANLQMNIWKPIGDNNTARPIILWIHGGGFFTGNKNDMDVIAQAYAERGYVS
                     ATMSYRLGFYGNLFFGAPFTFDSSEVIRAAYRAVQDAHGAMRFLKARAAIDSSNINLA
                     FVGGASAGSITAMHFTYVDKQSEAPINGGAIGDVTTLFGNVQRNDLGSIFGRLNLNGQ
                     SSNVLAAVNLFGALLDTSFIESNTDPALFSYHQTGDGTVGCGYMPGLWNQPMNVSQNY
                     PHLFGSCVINDRMNNLGFSSQHYQALLYNGNVHGIHDDALIDTLVARFLSDILCENIL
                     STNTVDMPDIDVYPNPAKGQINISIPGKTFTCSLFDVQGRNIIPSNSLLHDYITLNTE
                     GISKGMYLLNIITDKQLISKRIIIE"
BASE COUNT         4652 a         2608 c         3032 g         4639 t
ORIGIN      
        1 cataaaaatt attatgaaga agttatcatt actattatta tccattgcct gcatcttttc
       61 agtaaccact atggatgcgg caaaagccaa gaaaagcaag actgctaagg cagataaggc
      121 gtttaatgca ggtaagtatg cacctgcagc agatctttac aagaaagcct atggcaagca
      181 aaagtcgaag ttggtaaagg cggaaatcgc ctacaaagct gcagagtgtt acagtaaaat
      241 gagcaactct aaagaagctt taaattggta tgagaaatcg gctaaagaca atggtaaaaa
      301 tcctgatgcc gtattgaaat atgcacaggc attaaaaacc aatggccgtt atgatgaagc
      361 cgttgcacag tttaatgcat ttaaagcact tgaacctggt gataaccgtg gtcaggatgg
      421 agcaacatca tcagaaactt cacagcagtg gaaagataag ccaactaagt ataaagttga
      481 gaacgttgca gcattaaaca caaagtattt tgattttgct gttgcagtat caaacttaga
      541 gaaaaataca ctttatttta catcttcaag agcagaagca tcaggaaata aaaatgatgc
      601 atggtatgga gaaaaatttt atgacatctt caaatctgct caagataaca atggaaaatg
      661 gagcatccca acaccaatca gtgaagcaat taatactgct gcatcagaag gtgctatgac
      721 atttgatgca aaaggtaacg taatgtattt tacccgttgc cagaagagcg atgaaaaagg
      781 taaagaagga cagtgtaaaa tttacaagag cgtttatgac ggtgctaaat ggggtacacc
      841 agagttgcta ccatttaata gcgataatta tacttgtgga caaccttcaa ttactgaaga
      901 tggaaccaca ctttattttg catcagatat gcctggtggt gcaggtggaa aagacatctg
      961 gatgactaaa aatgaaaatg gagcatgggg tacgcctgta aacatctctt ctattaatac
     1021 tgtaggtgat gaaatgttcc catatattgc accaaacgga aaattgtatt tctcatcaaa
     1081 tggtcacatg ggcatgggtg gacttgatat tttctctgca acttcagaaa atggagcatg
     1141 ggcaaatgtt actaacttaa agtatccaat taactcatca tgggatgatt tcggatttat
     1201 gttcaattct gccactacag gttatttgac ttcaaaccgc gatggcggac aaggacagga
     1261 tgatatttat tcgttcgtag tacctcctgc aaattttgca gtttatggtc gtgtatatga
     1321 tacagacact aaagaagcaa ttgcaggtgc aacagttgaa ctttttggta gcgatggaac
     1381 acagctttct gttaaaacac aagatgcggg aacatatcgc tatgagttaa aacctgatgt
     1441 tgactataaa gtttcagcat catttacagg ctatctgaca caatttaaag aactgacaac
     1501 taaaggtctt gatgaatcaa aagacttcga acagaatttt gacttcccat taaagtcaac
     1561 ttcaaaacca attgcattac ctgaaatttt ctatgacctc gacaagtatt cattacgtcc
     1621 tgagtcaaaa gcagcattgg atggattaat taaagtgttg gatgataacc caactattac
     1681 cattaaactt actgcacaca ctgacttccg tgccgatgac aattacaact tggcactgtc
     1741 taaaaaacgt gctttagcag cttacaatta tttggtttct aaaaaggttg acaaagatcg
     1801 tttgagctgg gaaggaagag gagaaaaaga tcctaaagaa gttgagaatg acgagcagta
     1861 tctaccattc aaacgtggtg ataagttaac agaagagttt atcaaaggat taacttctga
     1921 agaactgaag gaaaaagctc atcagtacaa ccgtagaaca gaatttagag ttcttagtac
     1981 agactataca cccaaaaagg ctaagtaagc ccgattgaat tgtaaataaa aaagaggctg
     2041 tcttggttaa agacagcctc ttttgttagt taggattcag aatctcaaaa gagcttataa
     2101 taactaatag aataatctcc gccattttga acacacactt ttttaaattt gcagaggcag
     2161 acaaaacaga aaatgaaaga tcagaataaa tacagtcaac gtggtgtttc ggcagacaaa
     2221 gaagatgtgc atgcagcaat taaaaatgtt gacaaaggtc tttttccaaa agcgttttgt
     2281 aaaatcattc ctgactatat aagtaacgac aaagattatt gtttgctgat gcatgctgat
     2341 ggtgcaggca cgaagtcgtc attagcttat gcttattgga aggaaacagg agatatttct
     2401 gtctggaaag gaattgcgca agatgcgctg attatgaata ttgacgacct tctttgtgcc
     2461 ggtgctacag ataatatttt attaagctca acaataggaa gaaataaaaa tctgatttcc
     2521 ggtgatgtca ttgcggctat tattaatggc actgaacact tggttgctga gctgaaaaat
     2581 tacggagtta atattacaac caccggaggt gagactgcag atgttggtga cttggtgcgt
     2641 accattattg ttgacagcac cgtaatgtgc agaatgaaaa gaagtgaagt tgttgataat
     2701 gcaaatattg ttgccggtga cgttattgtt ggctttgctt ctttcgggaa agcaacttat
     2761 gagactgaat acaacagtgg aatgggtagt aatggcctca cttcggcaag acatgatatg
     2821 ttcaataaat cagttggcat aaaataccct gagacttttg ataattcact tcctgaagat
     2881 gtggtttata acggcaagta tgcattaacc gataacatta tacttgatga cgggtatgga
     2941 aaaattgaaa tgcatgcagc caaggcagtg ctatcgccta cgcgcactta tgctcccttg
     3001 gttaagaaaa tatttgaatc gtatagaaaa aatattcacg gcatgattca ttgcagtgga
     3061 ggaggacaaa ctaaggtgct tcattttgtt gataatcttc atatcattaa agacaattta
     3121 tttccgactc ctgctctttt tcgtcacata cagcaagcag ccggaaccaa ttggatagaa
     3181 atgtataagg tatttaacat gggtcatcgc ctcgaggtgt attgtcctgt agccatagca
     3241 gatgaattaa tttctattgc ggccgaattt aaaattggtg caaaggttgt cggacgtgtt
     3301 gaaaattgtg cgggtaaaaa acttaccatc aattcgccat atggaaaatt cgagtactag
     3361 ttattttttt gataacgggc atggcgttta ctcagttcat caataacgcc actataaact
     3421 gtgttcatca gttcaggatt tgcaagataa aagttcatgc tgtgggaaaa tacagcagta
     3481 tcaattctat gatgctcaaa aatatatttg taataagcag gaaggttaat gatgcctagt
     3541 gaatcattat atggatgcaa ggtagcctct gattcagcaa gatgaatgtc ggcaagtatc
     3601 tgtatcatgc tgtcttttga cagtgtacct tcaggaatta ttacttcttt atttttatga
     3661 gtgcaggata caatagataa agtcagaata gaaataagaa aaaagtattt tttcacttgc
     3721 atatattaat gagaagaaaa taataatcgc tgtccgttgg tgcttaattg gagatggcca
     3781 tcattgtaaa tgtgaacacc gtttacaaaa gtatgtttta ctttcgattg aaatgttgta
     3841 ccttcaaatg gcgaccatcc acatttatag aggatgttgc ttttggcaac agtccaaggt
     3901 tcattcaaat ttactaatga tacatcagca gcaaaacctt cttggataaa acctctgttt
     3961 ttaatattga aacatatagc cggtgaatga cacattttat ttgcaatctg ttccaaagaa
     4021 atttttccct gatgaaaaag ctccagcatg gcaacaagcg catgttgaac caatggccca
     4081 cctgaaggcg cttttagata gggcatttgt ttttcgtcta aagtgtgtgg tgcatgatca
     4141 gttgcaatga catcaatttt atcgctgagc agtgcgttta aaatagcttc tttgtcaact
     4201 tgtgttttta ttgcaggatt ccatttaata aaattacctt ttgaagcata atcgttatca
     4261 tcaaaccaaa gatgatgaat acatgcctct gaggttattc ttttatcttt aagtggaaca
     4321 ttgttagaga atagttctgt ctcgcgagcc gttgaaatat gaagaatgtg caaacgggtg
     4381 ttatactttt ttgcaagatt cacagcaaaa gaacttgaca ggtagcaagc ttcttcactg
     4441 cggataagag gatgatgtga tgcattaagt ttatctccgt aaagtgattt gtatttctct
     4501 gtattttgtc ggattgtctg ttcgtcttcg caatgtgttg caatgagcat tggcgattgt
     4561 gaaaatattt tatccaatgt agttggattg tccacaagca tatcacctgt tgaagatccc
     4621 ataaaaattt ttacgccaca aacattttct ctgtttgttt taagaacttc gtcaaggttg
     4681 ttgttgcctg cacccatgta gaaagaataa tttgcccacg attttttttg agcgagccgg
     4741 tatttttctt ctaataattc ttgtgtcaat gtgtttggta ctgtattagg catatccata
     4801 aacgaagtta tgcctcctgc aactgcagct gccgattcgg aagcaatatc gcctttatgt
     4861 gtaagtccgg gttcacgaaa gtgcacctga tcatcaataa tccctggaag aagtattaaa
     4921 ccatcacctt ttatttcatc aaccttatgt tttacattta tcgaagggtc aattctttca
     4981 atgatgccat tcttgaagta aatatcagaa tcgaaaattt tgttgtcgtt tacaattctg
     5041 acattatgaa taatggtttg catttgagtg aaaaaatttc tgctaaggta cgaatcagca
     5101 tggtttacca gtgtttagat tataaattca gaagaatgtt tttatgagtt tacatagtag
     5161 agataaaatt aattgcaaca tttttttatt tttgcatgct tataaaaaac accgttcggg
     5221 gtgtagcgca gcccggtagc gtatcagcat ggggtgctgg tggtcgctgg ttcgaatcca
     5281 gtcaccccga cagttttcat ctcaaagcgt tctgtaaaac agggcgcttt ttcttttcac
     5341 acataatttg tggatattat tcagcacctt atgtaaatta cttatcacag tcatttattt
     5401 ttcagtccga aatttaacac atggcattaa gcagaatttg gtcgtatatg attttatttg
     5461 ctgtagcagt agcaggctat cgttacatgc aaggtgacaa aaatattttt aatgacatgg
     5521 tggttgaaaa aaaattagct gccggtgaaa gtttcagtaa agtatcttat tatgtcattg
     5581 gtcagggaat tgacagtgtt ctcaaagaac caaaattaaa aaatgccaat tgggtaaaga
     5641 cagataaatt ctcagatgct caggtaataa tcaccactgc tgatttaaag aataatctgc
     5701 gcgagcaatt aaataatcaa cttgcaaaaa tcagttttgc gaatattaac actcaaaaca
     5761 aattcactgc tgacaaatcg aaattgattt attctgagga gttaattact gacagtacat
     5821 taaatataac aaacaatctg cttcaggcta atgttgttct tacatctgaa gtaaatacag
     5881 attcgcttcg aaatgcatgg acagctatca gcaacagaca aaaaattgtt ggtagcaatg
     5941 aagtatttct ctggcttcct aaaaacattg atggtatttt tgaaacagga aaatttgcag
     6001 tgactattgc cataggtctt ataggtacgc tggcattatt tatgggctta ctcaatattg
     6061 ctgaacgtgc aggtggcatc aacatcatca gccgattaat aagtccgttt ttgtcgaagc
     6121 tttttccttc gttgccaaaa aatcatccat cgtttggaca catggtgatg aacttttctg
     6181 ctaatatgct tggcctcgac aatgcggcta caccttttgg gcttaaggct atgcagagtt
     6241 tgcaagaaat taataccaac aaagacaggg caagcgatgc acagattatg tttctggcat
     6301 tacatgcatc agggttaaca ctgattcctg tttctataat tgctgttcgt tctgctaatg
     6361 gatcgcacaa tcctaatgag atttttattc cgctcatgct tacaactttt gtttgtactc
     6421 tcacagctat gattgtaacc tcgctcaaac aaaaaattaa tttgttccaa cgtggattgt
     6481 tgttacctgt tgccggactg agtttaacat taagtttgtt tgtatggttc ttaagcagcc
     6541 tgcgaccatt tgcattagat caattttctg atgcattcag caatggtgct attttattgg
     6601 tgttttttgg aattattgca ggaggacttt ataaaaaaat aaatctcttt gatgcattcg
     6661 ttgatggtgc gaagggaggt tttgaaacag cagttcgtat tattccttac atggtaggaa
     6721 tgcttgttgc ggtgagtatg ctcaggacaa gtggtgcgtt tgatttaatc attaatcctg
     6781 taaaacatct tattgcaatt tctggcatgg acacacgttg ggcagaaggt ttaccgacag
     6841 cattattacg acctttcagt gcaggtggtg caagaggatt tatgatagat gccatgcgca
     6901 catttggtcc tgattcgttt attggtaagt tagtgagcgt tttgcaaggc tcgagtgaaa
     6961 cgacatttta tgttgttgct ttgtattttg gctctgtttc tgtaaaagat acacgttatg
     7021 ctattcctgt tatgcttctg gccgacctgg ccggagtaat attctcaatc gtaatctgct
     7081 acttgttatg ggcataatct aacagtttag ttgttagcga gttacataca taaaaatccg
     7141 gtttaattta agttgatttt aaggccagaa ataggggcct actaatcaag tttttttata
     7201 ctttcgcaac ctcaaaaatt ttaaccgtag tattttatga gtcagatagt ttatgtagtt
     7261 ccggtgttgg gtttgttggc cctggtttat atgtttataa aagcctcgtg ggtaggtaag
     7321 caggatgcag gtgaaaagaa catgcaagaa cttgcaggtc acattgcaga tggagccatg
     7381 gcatttttaa aagcagaatg gaaaatatta tcctattttg ttgttatagc aggattgcta
     7441 ttggcttatt caggaacact acatggcaca gaagaatatc ctgttcattc aagcccgata
     7501 atagctgttg cctttgtgat aggtgcagtt ttttctgcag tagcaggcta tattggaatg
     7561 aacattgcca ctaaagcaaa tgtccgtaca gcacaggcag caagaagcag cctttcaaaa
     7621 gcattaaaag tttctttcac cggaggtgca gtaatgggac ttggtgttgc aggccttgca
     7681 gtactaggat taggttcgtt atttattgta ttctatcaat tgtttgttcc cacaggtgca
     7741 gcaattaccg gtgttgaaat gaaaaaagct attgaagtat tagcaggttt ttctctcggt
     7801 gcagaaagta ttgctttatt tgctcgtgtt ggcggaggta tttatactaa agctgccgat
     7861 gtaggtgctg acttagttgg aaaagttgaa gccggtattc ctgaagatga tccgcgtaac
     7921 cccgctacaa ttgcagataa cgttggcgac aatgttggtg atgttgccgg tatgggtgcc
     7981 gacttattcg gttcttatgt tgcaactatt cttgctacaa tggtattagg tcaggagatt
     8041 gacgcttctg ctgataagtt tggcgggctt tcacctattt tattaccaat gcttattgca
     8101 ggaatgggaa ttattttttc aattgtagga acatggtttg tgagaattaa aagtgaaaat
     8161 gataatgttc agaatgcatt gaacatgggt aactggtcgt caattgtact tacagcaatt
     8221 gccagttatt ttgccgtgac ttatttactt cctgaaaaat tagttatccg tggatttgag
     8281 ttcacaccta tgaatgttta tttagccata ttggttggtc ttgtagtggg agcattaatg
     8341 agcatcatca cagaatatta tactgctatc ggaaagcgac ctgttttatc aatcgttaaa
     8401 cagagctcaa ccggtcatgc cacaaacatt attggtggat tggcagtagg tatggaatca
     8461 actgcagtgc ctatgattgt tcttgcagga ggtatttttc tttcctattc attagcaggt
     8521 ctatatggcg ttgccatagc agcagcaggt atgatggcaa caacagctat gcagttggct
     8581 attgatgcct ttggtcctat tgcagacaac gcaggcggca ttgccgaaat gagtcagctt
     8641 ccaaaagaag ttcgtcagcg cacagataat cttgatgctg taggaaacac aactgctgca
     8701 acaggaaaag gttttgccat tgcatcggca gcattgacat cattagcatt gtttgcagcc
     8761 tttgttggtg ttgcaggcat ttcgcatatt gacatttata aagccaacgt gcttgcaggc
     8821 ttgtttgttg gtgccatgat tccttttata ttttcttcgt tggctattgc tgccgtaggt
     8881 cgtgctgcaa tgagcatggt agaagaggtg cgcagacagt ttcgcgaaat tccgggcatc
     8941 atggagtaca aagcaaagcc tgagtacgaa aaatgtgttg ctatctctac agaagcttct
     9001 atcagagaaa tgattgctcc gggtgcatta gcattaattg tacctgttgt agttggattt
     9061 attttcggac ccgaagtgct tggtggtgtg ttagcaggtg taactgtttc cggtgtgttg
     9121 atgggaatgt ttcagagcaa tgccggtggt gcatgggata atgcaaaaaa atctttcgag
     9181 aagggtgttg aaataaatgg tcagacatat tacaaaggct ctgagccaca taaagcatct
     9241 gttaccggtg acactgttgg tgatccattt aaagatacat caggaccatc aatgaatatt
     9301 ttgattaaac tgatgagtat tgtttctttg attattgcac cacacattgc atcaattggt
     9361 actcaggatt cttttaataa caagagcgaa gctaaaattg ttgctccggt tgtaacttct
     9421 acagtaaata gcacacctga aatggatctt gcagccatga caggaacaat tagccttgac
     9481 gtgagccact cctggatgca gtttggtata tcgcactttc tgacaacagc aaacggaaca
     9541 ttagcagtag atacaggtta tattaatatc actaatgact tggcaacaag ttcaattttt
     9601 gttcagttga atgtaaaatc tattgacaca aaaaatacaa agcgaaacaa tcatctttta
     9661 agctccgact tttttgatgc tgaaaaattc ccttcaatag tttttaaatc aaacaaaatt
     9721 gaaaaagccg cagagggctc agcatttcgt tatattgcta aaggtgagtt aacgattaaa
     9781 aatattacaa aacctgtaga gcttagattt aactacatgg gtgcattgcc aaatgaatat
     9841 gcagctaaag atgcacatac ttttgtgggc gaggcagatc ttgacagaat tgactttggt
     9901 gtaggcgaat cagggcctac agtaggtaat aaagtaaaag ctactttctc tgttgaaggc
     9961 tacaaaattt aaataaagag ttagtgtgaa ttcagaaagg ctgtcttaaa gggatggcct
    10021 tttttattgg cactaaaaaa tcgttattcg attaccatcc gcttgcaagc atatcggcaa
    10081 tatgcataaa ctgcaatgat gatccttcat tgtctgcaat actttttaaa tgcatcagac
    10141 aggtatagtc agtagagata actgtattag ctcctgtttg tgttaactcc agaatttttc
    10201 tcttaccaat atctactgaa atggcttcat acttttctgt aaatgatgga gaatagccgc
    10261 agcaactgtc tgtgctttca atccattcaa ggccttttac tttgctgagc agggtgcgaa
    10321 cagaatcttt tacagtgagt tcattaatgg ctttacagct atcaagaatt acaacctttc
    10381 cattaaaccg cgcaccaaca tcggttacat tgagtatgtt gactaaaaaa tctgtaaact
    10441 cataaatatt tttctgaaca cttttatatt cattgtgcaa tgctgagttg tgaaacatct
    10501 gcgggtaaat attttttatc attcccgaac agtaagcacc gggacaaaca atgtatcgct
    10561 cattctgaaa ctctttaatc aacttctcgc caacggcttt acattctttc tgataacctt
    10621 cttcaaatgc tgttctgccg caacatgttt gttctacatt atagttaacg ccacatccaa
    10681 gtttctcaat aattttcaca acattattgg ctgtttcagg aaaaacctga tccatgtagc
    10741 agggaataag taaatcaact atcattcttt atttgcaaat atccgataaa aaaatcaggt
    10801 gttttgtaat ctgaaatttc tgatgctaag taatgaaaaa agtcccacta caaaaaaagt
    10861 catcaaaact aaaattgaat tacgcatgga acctgtaagt tcttcaatat atccgtaagc
    10921 tactgttccc aataccaaac caattttatc acaaacatca taaaagctga aatacgaggc
    10981 atggtcctgt gttttcggta gcattttact ataagtggaa cgcgatagtg actgaatgcc
    11041 gcccataatc ataccaacag caaatgctaa tacaaaaaac tgcaccgatg tagccacaaa
    11101 atatgcagcc cagcaaatgc caatccaaat ggttactgca atcagcaagg ctttaatgtt
    11161 tcctgccttt gatgaaagaa atgcaaacag ataggcaccg gcaatggcaa caaactgaat
    11221 aatgagaaca gtgatgatta attcatcatc gcgcatgtgc agttccttgg tgccaaacaa
    11281 actggcaaca tacataacgg tttgcacacc catgttgtaa aagaagaatg aaatcaaaaa
    11341 tcgttttagc attggcaacg actgaagttg tttaagcact tttattaatt cattaaaacc
    11401 ttttgtcagt agttgttcat ttgtgttatg cgtactggtt ttttcaggaa gatatttaaa
    11461 tgtgaaaagt gaaaatccaa accaccaaac gcctgtcatt aaaaaagaaa ttcgtgaagc
    11521 catgcctgag ttggtaattc caaaccatga tggatttaat atcattgcca gattaaaaat
    11581 cagtaaaaga gagctgccaa tatagcctaa tgcaaacccc tgtgcactca ctttatcctg
    11641 catgtcggca gttgcaattt ctggtaaaaa agcattgtag aagacaatac ttccggccca
    11701 gccaatgctg gcaagaataa aagaaatgat tcctatatcg agatttgcaa ggtctttaaa
    11761 aaaataaagt gatgcacaag atgtagagcc aagaaaacaa aacagcctca taaaaaactt
    11821 tttatttcct ttataatctg caatgccact aagcagcggt gaaagaaatg ctatcactaa
    11881 aaaagaaaaa gatagggcat aagagtaaag tgctgtgttt gtgaaagtgc gaccgagaaa
    11941 tgttacctga tcgctaccgt tggcactggt aatgctgttg tagtaaatag gaaaaatagc
    12001 agtggtaata cttagtgaat aaactgagtt tgcccagtca taaaatgtcc aggcgcgtaa
    12061 aactttaggg ttgttttttt ccattaaaat caatgttgat ttattgccat ataaaattta
    12121 atgcaccatt ggtttgacct ccgttaaaca attgctggcc agcataagga agttgattag
    12181 tcataagcgg agtaagattt ttttctatca ctactgtttc cattaaatca tcagtttccg
    12241 gactgaagtt gtagttgaat actttaataa taattgactt gtcgaactgc gtaatggcaa
    12301 agggtgtaat aaaattaaaa tttgctgtgc cttgtggtgc attggtgctc actgttgtac
    12361 tgacataagt atttccattc caaatctgac agtaaatatc ggctgcagaa ccatcactat
    12421 cccatgtcgt tccatctgat ttatgaacag gccagctatt tagacttaga ctgacaatgt
    12481 tagcttttgt gtaaggtgat gcgttgatgc cttttattgt tgtagctgtt ttaccctctg
    12541 cagtttcgcc ttttaagaca acattataaa atccataatc aaatatgtgc attacgctat
    12601 cgccagaaac ttctgtgctg ccatcggcaa aattccattt atagttacaa ggaattgttg
    12661 attcagcttt aaaaataaaa gtgtccggat gtgttttgaa gtcattataa acagtgatac
    12721 ctaaaaaagg tgacggacta tcatcggact tttttttgca acctgagatt aaaaaactgg
    12781 ctacaaagat tggtaacaat atttgtgaaa gaaataatag ccaatttagt tttttaaatc
    12841 ctgtactgcg gcaatgccac agccgaaacc ccattatttt tttaagagta gttttttcca
    12901 ttcttcagga atgttttcgc tgacagaaag acggtaattg tagcaaacta aaacagtaag
    12961 cccatctgcc agaataattt tattgttatc agtagtttta aaaacttcaa agccaaggtc
    13021 aaaactcttt gttcctacac gagtgcacca tgttctgaca gataaattat cattcagcaa
    13081 tgcagggtgc cggtagttga tttgtgtctg tgcaagaata aatcctgttt cgcgccagtt
    13141 gatgtttaat ggcagacgtt cactaaaatc aattcttgct ttttcaatat agcttagata
    13201 aacggcattg tttacatgac ccatccagtc tatgtctgaa aagcggattt gaatgggggt
    13261 aattgtttcc gacatattta ttctatgatg atgcgtttag aaatcaattg cttgtcggta
    13321 ataatattga gcagatacat tccttttgaa ataccttcgg tatttagagt tatgtaatca
    13381 tgcaacagag aatttgaagg aatgatattt cttccctgaa catcaaacaa actgcaagta
    13441 aaagtttttc cgggtatgct gatattgatt tgtccttttg caggattggg ataaacgtca
    13501 atatcaggca tatcaacagt gttggtgctt aaaatatttt cgcaaagaat atcacttaaa
    13561 aaacgtgcaa caagagtatc aatcagcgca tcatcatgaa tgccgtgtac attgccgttg
    13621 tagagtaatg cctgatagtg ctgacttgaa aaaccaaggt tattcattct gtcatttatc
    13681 acacaagagc caaaaagatg cggatagttt tgagacacat tcataggttg attccaaagc
    13741 cccggcatat agccgcagcc aacagtgcca tcgcctgtct gatggtatga gaaaagtgcc
    13801 gggtctgtat tggattcaat aaatgatgta tctaacaaag cgccaaagag atttactgct
    13861 gcaagcacat tgctactttg tccattaaga ttcaaccttc cgaaaataga acctaaatca
    13921 tttctttgaa cattgccaaa gagcgtagtt acatcaccta ttgccccacc atttatcgga
    13981 gcctcgcttt gtttgtcaac ataggtaaaa tgcatggcgg tgatggagcc tgcactggca
    14041 ccacccacaa atgcaagatt aatattgctt gagtcaattg ctgcacgtgc tttaagaaat
    14101 cgcatggctc catgtgcatc ttgtacagca cggtatgctg ctctgataac ttcgcttgag
    14161 tcgaatgtaa atggtgcacc aaaaaacagg tttccataaa atcctaagcg atatgacatg
    14221 gttgcagaaa cataacctct ttcagcataa gcctgtgcaa taacatccat atcatttttg
    14281 tttccggtaa aaaaacctcc gccatgaatc cataaaatta tagggcgtgc tgtgttgttg
    14341 tcgcctatgg gtttccagat gttcatttga agattagcca ctgtattgtt ataggcagtg
    14401 tcgcttccgt aacttatatc tctttcaaca taggtttggt agattctcgg ataataaggg
    14461 tcaagacatt gcgtaaagct ttgtccgtaa gaaataacgg caagcattaa aagtaaaatt
    14521 ctattcatca taaaaaggtt attaaaatct gttgactaaa gtatggttaa tggatggcat
    14581 ttgcaaaggg tgttttttga gtgattgggt aaagcgagat aatcaaccac agctgataag
    14641 ggtaataaag ttaattagat aatgagataa tttgataatc agccacagct gacaagtttg
    14701 ataattcaga attataggtt ctgcatagtt tcattatcat tatttcatac cgtaggcgcg
    14761 agcgtttttt ccgctcgtgc cgtttatttt gtttgcatct tgcaaactat aaaacttatt
    14821 ttgccggtgc atggcgaaaa acaaaagata tttcctgacg ggttatagtt tgttacatta
    14881 accacagttg gttggattga tgtttttaca agagccattt actgcgatga g
//
LOCUS       DAOOOI010000051        14849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_806_length_14849_cov_29.373395, whole genome shotgun sequence.
ACCESSION   DAOOOI010000051 DAOOOI010000000
VERSION     DAOOOI010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14849
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_806_length_14849_cov_29.373395"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1545)
                     /locus_tag="PKX59_07415"
     CDS             complement(82..1545)
                     /locus_tag="PKX59_07415"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOZ90731.1"
                     /translation="MQTTATKDVNYEQDLNEWIKAEKAAIELIHVTGSLWFDKSVELV
                     MFRNQMIDRSSSQLLNLIQYSKEIVKKPINIFDALAIAKELNAIDLAPSRLDLGRLAH
                     EWLVEKEKYKTVNKFIADKLKDFIGKGKKTLTPKDVVLYGFGRIGRLLARELTIMAGK
                     GEQLRLRAIVTRDKSDEDIIKRADLLRTDSVHGPFPGTVIEDIQNRALIINGHTVHMI
                     SAKNPEDIDYTKYGINNALLVDNTGVARDREGLGKHLLSKGVDKVLLTAPGKGDIPNV
                     VYGVNQTQHDANEKIYSAASCTTNAIVPVLAVINKTLGIERGHIETIHSYTNDQNLLD
                     NYHKKYRRGRSAALNMVITETGADKAVSKALPELAGKITGNAVRVPTPDVSLAILNLT
                     LGKKSDKDSINEILKDAALKGDLVEQIQYSYGNELVSTDLVGNPCASIVDSPATIVSK
                     DGKTAVLYVWYDNEYGYSRQVIRFAKYLADVIRLTYY"
     gene            1760..1924
                     /locus_tag="PKX59_07420"
     CDS             1760..1924
                     /locus_tag="PKX59_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90732.1"
                     /translation="MKQLLYIFITQWADKTISLVGLVLFKTSVLAGLLSFTSKKRKEN
                     YKIVIEKEKL"
     gene            complement(1932..2357)
                     /locus_tag="PKX59_07425"
     CDS             complement(1932..2357)
                     /locus_tag="PKX59_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90733.1"
                     /translation="MNKTKITNYKELLAAKEVLQKRSLDLEEKLTANINTLKSELTPA
                     NILNKTFKALLFENKAGITGSALRLGAEYLIGRSAQRKSGSALYGTLGYVAAKSLVNY
                     TLENKKYLLNTLSRLLNKIDLKKNEPNNIFDQSTADDHF"
     gene            complement(2350..2715)
                     /locus_tag="PKX59_07430"
     CDS             complement(2350..2715)
                     /locus_tag="PKX59_07430"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOZ90734.1"
                     /translation="MPPEKDTLKALYEDAREYVEVKLELTYLNFQQKLSEIVSSLTVV
                     FVIAILALVVFAFLSVGLALLIGQYLNSIYAGFFIIALFFLIVLVCIAVFSQRLIKEP
                     LTDFIIRRLNGSEKNAADE"
     gene            complement(2730..2960)
                     /locus_tag="PKX59_07435"
     CDS             complement(2730..2960)
                     /locus_tag="PKX59_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOZ90735.1"
                     /translation="MSNGSKILGALLIGAAAGAILGVLFAPDKGEETRRKIRKKGEDI
                     LDDIKRKINEANDEIDNLSKEMEDGNPSANRY"
     gene            complement(3055..3375)
                     /locus_tag="PKX59_07440"
     CDS             complement(3055..3375)
                     /locus_tag="PKX59_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90736.1"
                     /translation="MSTDRLQQLEAMYAENPKELFVLYALALEHVKLGNEEQSLEYFE
                     NVLSVSRSYLPVFYQYAVFLHDKDIQKAIMLCHEGIMLASKQNKSKTKVELLSLLAYL
                     SDNN"
     gene            3453..4190
                     /locus_tag="PKX59_07445"
     CDS             3453..4190
                     /locus_tag="PKX59_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOZ90737.1"
                     /translation="MKILVCISHVPDTTTKISFTADNKSIATSGIQYIINPYDEIALT
                     RALELTEKSGGTVTVINVGDASTDATLRKALAIGATDAVRVDANPANASFVAAQIADY
                     ASKNSFDLILTGRESIDYNGSQVAAMVGELLNIPSVSIVKSLEVADKNVTLEREIEGG
                     KEIVSCPTPLVASATEGMAEPRIPNMRGIMSARTKPLQVMAAVATETYQQLVGYDKPE
                     PRTQVKLIDASNVKALVDALHTEAKVI"
     gene            4216..5193
                     /locus_tag="PKX59_07450"
     CDS             4216..5193
                     /locus_tag="PKX59_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOZ90738.1"
                     /translation="MNVLVYIETAEGKLKKSATEVLSYGKAIAKNSGGMLTALAIGDL
                     AADEQSKAGRYGADKVLHVTNDKLKQFINQAFASVVAEAAKQSNTSVVVISNTFNGKA
                     MAPRVAVKLKAGMVDGAVGLPDLNNGFKVRTSAFSGKAFADIELTSAIKVITVSPNSF
                     KAEENQAAGTIEAFSPSVADSDFTAMVKEIVKASDKISLPEAEIVVSAGRGLKGPENW
                     GMIEELATLLGAATACSKPVSDAHWRPHSEHVGQTGLAISPNLYIAIGISGAIQHLAG
                     VSSSKTIVVINKDPEAPFFKAADYGIVGDAFEVIPKLIEEVKKFKAANG"
     gene            5227..5844
                     /locus_tag="PKX59_07455"
     CDS             5227..5844
                     /locus_tag="PKX59_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOZ90739.1"
                     /translation="MKKIRLDIFGLSYSQAQSGAYALVLNEQGGTRRLPIIIGGYEAQ
                     AIAVELENMTPSRPLTHDLFKNFSISFGIHIDEVTIYNLKEGVFYSKIVCNNGEKQLE
                     IDARTSDAIALAVRFNCPIYTFDFILAQAGVEMDEDLGTATSSEMPKIEKPAPAPRKK
                     TKSIGSNDFSHLSTEELNEALINAIDNEDYEAASKIRDELTRRKK"
     gene            complement(5898..6827)
                     /locus_tag="PKX59_07460"
     CDS             complement(5898..6827)
                     /locus_tag="PKX59_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOZ90740.1"
                     /translation="MKISNEVKVGIAFTLTIAGFFWGMNFLKGTDLFSTSNKYYVVYD
                     DVNGIVKSNPVIMNGFKIGQVDKIVYAEDKSGRLIVTLRVDDNVFIPKSSNAAIVSSD
                     LLGSKAIELEMSEDKTPAKNGDTLVGNVQSGLTDQIKPLKNKAEGLVTSLDSLSYALT
                     QLINERSRNSLNNIFANLENTTEGLDKMVNSSNSKLNSMLSNIDGVAGNLNNKQSDIN
                     GILKNVNSLSDSLSRLQLNLTIEKLNKTIAELNSVLGKIDNSQGSLGKLVNDDSLYNN
                     LNNASANLDKLLIDLKANPGRYVNISVFGRKNK"
     gene            complement(6839..8230)
                     /locus_tag="PKX59_07465"
     CDS             complement(6839..8230)
                     /locus_tag="PKX59_07465"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOZ90741.1"
                     /translation="MLFIYKKIKIRLFFTLSLLFLICVSASNGTYKEPYRIKTIVIDP
                     GHGGHDSGCLGASSKEKQIALEVALKLGEMITQKYPDVKVIYTRKTDEFVELHERAAI
                     ANRNHAELFICIHCNSGSPAAFGAETYVMGLHKTEDNLNVAKRENAAVLLEKDYKQKY
                     DGFDPNSPEANIVFTLYQNAYLTQSLKFASAVQQQFEVYAGRYNRGVKQAGFLVLYKT
                     AMPSVLIETGFLTNRTEEKYLLTEKGQNAVATSIFRAFMEYKLDMETPGGKIPQGGSE
                     NDLNKNVIEVKNKQSDKESSQPQKKVTDSEKNPPTTNSTKENHEVNKDTTKKNTDSAD
                     TQKETPPSENYKTETAVKPVASKEKITTAEVKKETAHTDKLPDFFYTVQLAAMPDAST
                     EELKRFEDLTDIKTFKAENGMIRVCQGVYTTHAKALAAQSQVRAKGYKDAFVTPYYKG
                     KRISNKEAEAIKK"
     gene            8381..10855
                     /locus_tag="PKX59_07470"
     CDS             8381..10855
                     /locus_tag="PKX59_07470"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOZ90742.1"
                     /translation="MFSTENIHAQTLPKDSLSTDTLAKDTIKTGVSNDLKSKVHYTAD
                     DTIEFDIEHEKVFLYEKAQVNYENIELKAAYIEVDWNTKNVFSAGLRDSTGKLYGMPV
                     FKEGDDEFKADTMRYNFDTKKGKVYHVVTQQGDGFIHGAIVKKIEESSYIRYGKYTTC
                     SLDTPHYYIAANKLKVIPNNKIITGPAYLIIGDVPAPAAIPFGLFPNTKGRSSGILFP
                     AYGESNALGFFFKNGGYYFGISDHVDLQLTADIYTLGSWAVKATSRYNNRYHYNGNLS
                     FLYSIIKVSEKDLPDYSRSKEFFINWSHLQDPKARPNGIFSASVRAGSNDYYTRNISS
                     ASNYLTNTFNSSISYSRSFFNKQLNFSSAITHSQNTQTRMVYLTAPSINLSMNTLYPF
                     RKKEQEGTLAWYEKISVGYSSTLQNQLQNPDSLFCKNTTLQQMKNGMQHNIPISASFK
                     AMKYFNLTPAINFNERWYIETYKQRFDADSNRIMTDTVKGFNAAHEYNGSLSLNTRIY
                     GMKQFKKGKIAAIRHVLSPTLSYMYKPDFGENKYGYYKTVQTDSIGNSRRYSIYENTL
                     YGGPSQGKSNILSFSLDNNLEMKTRQYTDTAVNIKKIKILESFGLSASYNLSADSFNL
                     SPIGMNARTTLFDMVSLTASGTFDPYLMNDQGIRIAKYSLTENGKIARLTSASGSVGF
                     NLNRQKGNKKSLKGTVEQVKEINDHPDDYVDFSVPYNLSVNYSIAYSRPTNEKGNLSQ
                     IVGFNGDISLTEHWKITFNSGYDFQTKKQSYTSLGFYRDLHCWEMRLNWVPFGYQANY
                     YFQINVKSSVLQDLKLTKKNDRYDNF"
     gene            complement(10858..11772)
                     /locus_tag="PKX59_07475"
     CDS             complement(10858..11772)
                     /locus_tag="PKX59_07475"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOZ90743.1"
                     /translation="MNFELTILGSSSATPIHQGHPTAQVLNIRERFFMIDCGEGTQGQ
                     FLKYKFKLNKLSHIFISHLHGDHYLGLVGLLSTMHLQGRTTEIHIYGQPELMDVIELQ
                     LRLSQTRLRYQLIFHPVQHYAPAIIYEDDDMLIKSIVLNHRIPCTGYLFSEKPKPRKL
                     IISKLQQHNIPFSFYGRLKDGFDYEDDEGKIIPNTELTISSTAPRSYAFCSDTCYDES
                     IIDEIKGVNLLYHEATFMNDLAERAKTTFHSTSLQAATIAKKAGVDKLLVGHFSARYR
                     DLKPLLDEARTVFKNTELAIEGHKFSVE"
     gene            complement(11772..12542)
                     /locus_tag="PKX59_07480"
     CDS             complement(11772..12542)
                     /locus_tag="PKX59_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90744.1"
                     /translation="MIKKLLFSLMFISMSGIAVSQCTPDPTIIDPGVYPDSATGLSNG
                     MVGVAYNQVIQVRTFFDTTATINIGGVPFNANFELDSITILGVSGLPPGLTYACNPGT
                     CQFLASSNGCLLLSGTPVTAGVYPITVDIIARGKITNLGNISYGQPMSVTYYTITIDT
                     NSGVIEMLPKDKLSMSQNLPNPFKNKSVVYFNSPVNTKLDFKIYNILGKNIFSETISA
                     KRGVNTYTINAENLSSGIYMYSINTGTANITRRMVISK"
     gene            complement(12560..12916)
                     /locus_tag="PKX59_07485"
     CDS             complement(12560..12916)
                     /locus_tag="PKX59_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOZ90745.1"
                     /translation="MHYTLDKKEKYAVLKLHEQKLNTLISAELKSELLILNSQGFIHI
                     VLDLSDTQYCDSSGLSAILVGNRICRNANGNFIVTEISDPVKKLINISQLDSVLTIVP
                     KIDEALALINKAASVS"
     gene            complement(12998..13927)
                     /locus_tag="PKX59_07490"
     CDS             complement(12998..13927)
                     /locus_tag="PKX59_07490"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOZ90746.1"
                     /translation="MGKLSVKHLIGIKDLKPEDINLILDTAQTFKEVINRPIKKVPSM
                     RDVTVVNLFFENSTRTRLSFELAEKRLSADILNFTASSSSVSKGETLIDTVNNILAMK
                     VDLVVMRHPKPGAALFLSQKVNSVIVNAGDGTHEHPTQALLDAFSIREKLGTLKGKKV
                     VIVGDILHSRVALSNIYCLQKSGAEVMVCGPATLIPKYIEQLGVKVETNLINALNWCD
                     VANVLRIQLERQDIKYFPSLREYIMQYGITLDILKKLQKKIVIMHPGPINRGVEITTE
                     VADSDQSIILDQVENGVAVRMAVLYLLAGQPVG"
     gene            complement(13908..14477)
                     /gene="pyrR"
                     /locus_tag="PKX59_07495"
     CDS             complement(13908..14477)
                     /gene="pyrR"
                     /locus_tag="PKX59_07495"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOZ90747.1"
                     /translation="MTHLILAGKQLDITIERLCHQLIENHGNFKDTCIIGLQSKGVPL
                     SKRICKRLSEFLPESSILHGALDITFFRDDYRRSEKLLVPSRTEIDFIVEGKKVVLVD
                     DVLFTGRTVRAAMDALMAFGRPAKVELLVLIDRKFSRQFPIEPDYTGQAIDTTITDKV
                     QVEWKESGNKDGVWLVSEIKSKTNGKAKR"
     gene            complement(14593..>14849)
                     /locus_tag="PKX59_07500"
     CDS             complement(14593..>14849)
                     /locus_tag="PKX59_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019943831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HOZ90748.1"
                     /translation="FSDEILGVQFHPEADAEGMYRYFDRPDKKEKIMELYGSEKYYDM
                     IESLSDPNKIELTHHAIIPCFLADAIEHLYPQLKMQKATV"
BASE COUNT         4598 a         2804 c         2637 g         4810 t
ORIGIN      
        1 aaagttagtt gtctttaagg agtagtaaaa aaaagcttcc ccatttagag aaagcctttt
       61 gttttttgta aatcttaaaa cttaatagta tgtcagtctg ataacgtcag caagatattt
      121 tgcaaaacga attacctgac ggctatagcc atattcgtta tcataccaaa cataaagcac
      181 tgctgttttt ccgtctttac ttacaatagt agcaggacta tcaacaatgc ttgcgcatgg
      241 attacccaca aggtcggtgc ttactaattc attaccgtac gagtattgaa tttgctcaac
      301 caaatcacct ttaagtgctg catctttcag gatttcattg atggaatctt tatctgattt
      361 ttttcctaat gtcaggttca gaattgctaa ggatacatca ggagtaggaa cccttacagc
      421 attgcctgta attttaccgg ccaattcagg taaagctttt gacactgctt tatctgcacc
      481 tgtttcagta attaccatat ttaaagcggc cgaacgacct cgtctgtatt ttttatgata
      541 gttatcgaga agattctgat cgtttgtata agagtgaatt gtttcaatat gtccacgttc
      601 gatacctaat gtcttattaa taactgccaa caccggaact atggcattgg tagtacaaga
      661 tgctgcagaa tagatttttt catttgcatc atgctgtgtt tgatttacac cataaacaac
      721 attaggaata tctcctttgc cgggagctgt taacaatact ttatcaactc cttttgaaag
      781 taaatgttta cctaagcctt cacggtcacg tgcaacaccg gtattatcaa ccagcagcgc
      841 attgttaatc ccatactttg tataatcaat atcttcagga ttctttgctg aaatcatgtg
      901 tacggtatgt ccattaatta tgagagcacg gttttgaata tcttcaatca cagtgcccgg
      961 aaatggccca tgaacactat ctgtacgtaa caaatctgca cgtttgatta tatcttcatc
     1021 acttttatca cgggtaacta ttgcacgtaa acgaagctgt tcgcccttac cagccatgat
     1081 agttagctcg cgcgccagta acctgccaat ccgaccaaaa ccataaagaa caacatcctt
     1141 aggtgttaat gtctttttgc ccttaccgat aaaatctttt agtttatcag caataaactt
     1201 atttactgtt ttgtatttct ctttttcaac gagccattca tgcgccaaac gtcctaaatc
     1261 gagacgtgat ggtgcaagat caatagcgtt taactctttg gcaatagcta aagcgtcaaa
     1321 aatattaatc ggctttttta caatttcctt agaatactga atcaggttca acaattggct
     1381 gctactgcga tcaatcatct gattgcgaaa cataaccaat tctacagatt tatcgaacca
     1441 taaagatcct gtaacatgaa tcaactcaat ggcagctttt tcggccttaa tccattcgtt
     1501 cagatcctgt tcgtagttta catcttttgt tgctgttgtt tgcatttttg tttgatttta
     1561 ttcaggctgc aaatatactt catattcaca atcatttttc agcaaaattg caccatttat
     1621 tacattataa ctgctttgtg attcagtact ttaaaatgaa ttgcagatta actacacaac
     1681 ataatgttaa aaacattttt ctgtttaatc cgtttaatgg gctaattgat ggacttttgt
     1741 agtcagaatt ttaaggtgga tgaaacagct tttatacata tttattacgc agtgggcaga
     1801 taaaacaatc agtctcgttg gattggtatt atttaaaact tctgtacttg caggtctgtt
     1861 aagttttaca tccaaaaaga gaaaagaaaa ctataagatt gtgattgaaa aagaaaagct
     1921 ttaagagtga atcagaaatg atcatcagcc gttgattgat cgaaaatatt gttcggctca
     1981 ttttttttca agtcaatttt atttagcaat ctacttaatg tattgagcaa atactttttg
     2041 ttttcaagag tgtagttgac cagtgatttt gcagctacat atcctaacgt accatacaat
     2101 gctgaaccac ttttacgttg tgcgcttctg ccaattaaat attctgcacc aagccttaat
     2161 gcactacccg taatacctgc cttgttttca aacaataatg ctttaaatgt tttattcaga
     2221 atattggcag gtgtcaactc actttttaat gtattgatgt tggcagttag tttttcttct
     2281 aaatctaaac tgcgtttttg caagacttct tttgctgcca gcaactcttt gtagtttgta
     2341 atttttgtct tattcatcag cagcattttt ttcggaacca tttagtcgtc tgataataaa
     2401 atcagttaac ggttctttaa tcagtctttg tgaaaacaca gctatacaaa ccaatacaat
     2461 aagaaagaat aatgcgataa taaaaaagcc tgcatatatg ctatttagat attgcccaat
     2521 caataatgcc aatccaacac ttaaaaatgc aaaaacaacc aatgcaagta tggcaatgac
     2581 aaatacaact gtgagtgacg aaacaatttc agaaagtttt tgctgaaaat taagatatgt
     2641 cagttctagt tttacctcaa catattcccg tgcatcttcg taaagtgcct ttagcgtatc
     2701 tttttccggg ggcatatttt aaattacgtt caatatctgt ttgccgatgg gttgccatcc
     2761 tccatttcct tggagaggtt gtcaatctca tcattagctt cattgatttt tcgtttgata
     2821 tcatcaagga tatcttctcc tttttttctg atttttctgc gtgtttcttc gcctttatcg
     2881 ggggcaaata atactccgag aatagcaccg gcagcagcgc ctataagcag cgcacctaat
     2941 atttttgaac cattagacat aacgttatat ttttaaattt tatactacaa agataatcac
     3001 cctttacagt ttattgatga atattttagt taaacaccaa ttgtgtgtaa ttgttcagtt
     3061 gttatcactc aaataagcta aaagtgacaa cagttccacc ttggttttgc ttttgttttg
     3121 ttttgatgca agcataatac cttcatgaca taacattatt gctttctgga tatctttatc
     3181 atgcaagaat actgcatact gataaaaaac aggtaaataa cttctgctta cagataaaac
     3241 attctcaaaa tattctagag attgctcctc atttccaagc ttaacatgtt caagtgcaag
     3301 ggcatataaa acaaataatt cctttggatt ctcggcatac atagcctcaa gttgctgaag
     3361 tctgtctgtt gacatttcat tcacatttat ttcattgaag tacaataata cctatttttg
     3421 cattccgaaa aaagtagtta atcattttaa atatgaaaat tttagtttgt atcagtcacg
     3481 tacccgacac aacaaccaaa ataagtttta ctgccgataa taagtctatt gctacttcag
     3541 gcatacaata cattattaat ccttatgatg aaattgcact tacccgtgca ttggaactga
     3601 ctgagaaatc aggcggaacc gttactgtaa ttaatgttgg tgatgcaagt actgatgcta
     3661 ccttgcgcaa agctttagct attggtgcaa cagatgcagt gcgtgttgat gccaatccgg
     3721 ccaatgcttc ttttgttgct gcacaaattg cagactatgc atcaaaaaat agttttgatt
     3781 taatcttgac cggtcgtgaa tctattgatt acaatgggtc gcaagttgca gcaatggttg
     3841 gtgaactttt aaacatccct tctgtaagca ttgtaaaatc tcttgaagta gctgataaaa
     3901 atgttaccct tgagcgcgaa atagaaggtg gtaaagaaat tgtttcatgc cctactccac
     3961 ttgttgccag tgcaacagaa ggaatggcag agccacgcat tcccaacatg cgcggaatta
     4021 tgagtgcacg cacaaagcct ttgcaggtta tggctgctgt tgcaactgaa acctatcagc
     4081 agttggtagg atatgataaa cctgaaccac gcacacaagt aaaattaatt gatgcatcaa
     4141 atgtaaaagc acttgttgat gcattacaca cagaagcaaa agttatttaa aaataaaaat
     4201 ttgagaattt aagatatgaa tgttcttgta tatatagaaa ctgcagaagg aaaactgaaa
     4261 aaatcggcaa cggaagtttt aagttatggt aaggcaattg ccaaaaattc aggtgggatg
     4321 ttaacggctc ttgctattgg tgatttggca gctgatgagc aaagcaaagc aggccgctat
     4381 ggtgctgata aagttttaca tgttacgaat gataaattaa agcaatttat aaatcaggct
     4441 tttgcttcag tagttgcaga agcagcaaaa caaagcaata catctgttgt agtaatttca
     4501 aatacattta atggaaaggc tatggcacca cgtgttgccg ttaaacttaa agcaggtatg
     4561 gttgatggcg ctgttggttt acctgattta aataatggtt ttaaggtaag aacttcagct
     4621 ttttcaggca aagcatttgc tgacattgag ttgacatcgg ccataaaagt aattactgtt
     4681 tctccaaaca gttttaaagc agaagaaaat caggctgcag gcacaattga ggcatttagt
     4741 ccatctgttg ctgacagtga ttttactgca atggtaaaag agattgtaaa agcatcagat
     4801 aaaatatcat tacctgaagc tgagattgta gtttcagcag gtcgtggttt aaaaggaccg
     4861 gaaaactggg gaatgataga agaacttgca actttattag gtgctgcaac agcatgttct
     4921 aagcctgtta gtgatgcaca ctggcgtccg catagtgagc acgtagggca aacggggtta
     4981 gctatcagtc ctaatttata tattgctatt ggcatttcag gtgctattca acatcttgca
     5041 ggtgtaagtt catcaaaaac cattgttgtt atcaataaag atccggaagc accattcttt
     5101 aaagctgccg attatggtat tgttggtgat gcttttgaag tgataccaaa acttattgaa
     5161 gaggttaaaa aatttaaagc cgctaatggc taatcatatt aaaagtttgt ttacttttaa
     5221 accttgatga aaaaaataag actcgatatt ttcggtttgt cttatagtca ggcacaaagt
     5281 ggcgcctatg ccttggtatt gaatgagcaa ggtggcacac gaaggctacc tataataatt
     5341 ggcggttatg aagcgcaggc tatagcagtt gagttggaaa acatgacacc cagtcgcccg
     5401 ttgacacacg atctttttaa aaatttttca atctccttcg gtattcatat tgatgaagta
     5461 acaatctaca atctgaaaga aggtgttttt tattcaaaga ttgtctgcaa caatggcgaa
     5521 aagcaacttg aaattgatgc ccgcaccagt gatgccatag cgctggctgt aaggttcaat
     5581 tgtcctattt atacatttga ttttatattg gcacaagccg gtgttgaaat ggacgaggat
     5641 ttaggaacag caacaagcag tgaaatgcct aaaatagaaa agcctgcacc tgctccaaga
     5701 aaaaagacaa aatcaattgg cagtaacgat ttttcgcacc tttctacaga ggaattgaac
     5761 gaggcattga ttaatgccat tgacaacgaa gactatgagg ccgcctctaa aatcagagat
     5821 gaattaacga gaaggaaaaa ataaactttt gccaaaagtg agcttcttaa tgcgtgcata
     5881 tttttataca ctctctccta cttatttttc ctaccaaaca cagaaatgtt aacatatctt
     5941 cccgggtttg cctttaaatc tataagaagt ttgtcaagat tagctgatgc attgttaagg
     6001 ttattgtaaa gactgtcatc atttaccaat ttccccaaac taccttgcga gttatctatc
     6061 ttgcctaaca cactatttag ttcggcaata gttttattga gtttttcaat agtgaggttt
     6121 agctgtaacc tgctgagtga atcactgagc gagttaacat tttttaaaat accattgatg
     6181 tcggattgtt tgttgtttag gttacctgcc actccatcaa tatttgaaag catactgttg
     6241 agtttgctgt ttgaactgtt caccatttta tccaaacctt cagttgtatt ttcaaggtta
     6301 gcaaaaatat tattcaaact gttgcgtgat ctttcattga tgagttgcgt taaagcatac
     6361 gataatgaat caagagaagt caccaaccct tccgctttat tctttagtgg ttttatctga
     6421 tctgtcaatc ctgactgaac atttccaact aaggtgtcgc catttttagc aggtgtttta
     6481 tcttcactca tttcaagctc tatggctttt gagcctaaca aatcacttga aacaattgct
     6541 gcatttgatg attttggaat aaatacatta tcatcaacac gaagtgtcac tattaacctt
     6601 ccacttttat cttcagcata aacaatctta tctacctgac cgattttaaa cccgttcatt
     6661 ataaccggat ttgattttac aataccattt acatcgtcat aaacaacata atatttgttt
     6721 gaggtagaaa acaaatctgt tccctttaaa aagttcatac cccagaaaaa accggctatg
     6781 gtaagtgtaa atgctatgcc tacttttact tcgttagata ttttcacgtg atctgaattt
     6841 attttttaat tgcttctgcc tctttatttg aaattcgttt gcctttatag taaggagtaa
     6901 caaatgcatc tttatatcct tttgcccgaa cctgtgactg tgctgccaat gcttttgcat
     6961 gtgttgtata aacaccctga caaactctta tcattccatt ttcggcttta aatgttttaa
     7021 tgtcagtcag gtcttcaaat cgttttaatt cttctgttga tgcatcaggc attgcagcaa
     7081 gttgtacggt ataaaaaaag tcaggtaatt tatctgtatg tgcagtttcc tttttcacct
     7141 cagcagtggt aattttttct tttgaagcaa cgggcttaac agcagtttct gttttgtaat
     7201 tttctgatgg gggtgtctct ttttgagtat cagcactgtc tgtgtttttc ttggtagtat
     7261 ctttatttac ttcgtgattt tctttggtgc tgtttgtagt ggggggattt ttttcactgt
     7321 ctgtaacctt tttctgtggc tgagaagatt ccttatctga ttgcttattc tttacttcaa
     7381 ttacattttt atttaagtca ttctctgagc ctccctgcgg tatcttgccg ccaggagttt
     7441 ccatatctaa tttatactcc ataaatgctc tgaaaatact tgtagcaaca gcattctgac
     7501 ctttttcagt aagtaaatat ttctcttctg ttctgttggt taaaaatccg gtttcaatca
     7561 gaactgatgg catagcagtt ttataaagta ccaaaaaacc agcctgctta acaccacggt
     7621 tataacgccc tgcatagact tcaaactgtt gttgaacggc agaggcaaac ttcaaactct
     7681 gagtcagata agcattctga tacaatgtaa acacaatatt ggcttcaggc gagttggggt
     7741 caaagccatc atacttttgc ttgtagtcct tttccaacaa cacagcggca ttttcgcgtt
     7801 tggcaacatt aagattatct tctgttttat gtaaacccat aacataggtt tctgcaccaa
     7861 aagctgctgg gctaccggag ttgcaatgta tgcagatgaa aagttcggca tgattacgat
     7921 tggcaatagc agcacgctca tgcaattcta caaactcatc tgtttttctt gtgtaaataa
     7981 ccttcacgtc agggtatttt tgggtaatca tttcacccaa tttaagtgcc acttcaagtg
     8041 ctatttgttt ttctttagat gaagcaccca gacagcctga atcatgccca ccatgaccgg
     8101 ggtcaataac aatggtcttt atgcgatatg gttccttata tgttccatta gacgctgaca
     8161 cacagattaa aaacaacagt gaaagtgtaa aaaagagccg tatttttatc ttcttataaa
     8221 taaacaacat gattctgcgt ttctttggat ggtgttaaat tctgttcaat tacctttgcg
     8281 gctccaaaaa tatcgttttt aactccatct cctttttttg taaatgaccg tttttaaaaa
     8341 cttattaacg attgcaatat tgtggtgcct ggcggtcatt gtgttttcaa ctgaaaatat
     8401 tcatgcacaa acgcttccaa aagactcatt atctactgat actcttgcca aagacaccat
     8461 taaaaccggt gtgagtaatg atttaaagtc caaagttcat tacactgctg acgatactat
     8521 tgaatttgat attgaacatg aaaaagtttt tttgtatgaa aaggctcagg tcaattatga
     8581 aaatattgag ctgaaggctg cttatattga agtggactgg aatacaaaaa atgttttttc
     8641 ggctggattg cgcgattcaa caggaaaact ctatggaatg cctgttttta aagaaggtga
     8701 tgatgagttt aaagccgata caatgcgcta taattttgac accaagaaag gcaaggttta
     8761 tcatgtcgta acacagcagg gcgatggctt tattcatggt gcaatagtta aaaaaataga
     8821 ggagagcagc tatatacgtt atggcaaata cactacctgt agtttggata caccacacta
     8881 ttacattgcc gcaaacaaac tcaaagtaat tccaaacaat aaaattatta ccgggcctgc
     8941 ttatctgatt attggagatg ttcctgcacc tgcagccatt ccattcggat tatttcccaa
     9001 taccaaaggt cgtagttccg gaattttatt tcccgcatac ggtgaaagca atgcacttgg
     9061 atttttcttt aaaaacggag gctattattt tggtattagc gaccatgttg acctgcaact
     9121 tacagcagac atctatactt taggtagttg ggctgtaaaa gcaacttcac gatacaataa
     9181 ccgttatcat tacaatggaa atttatcgtt cctatactct atcataaaag taagtgagaa
     9241 agatttacca gactatagcc gtagcaaaga atttttcata aactggagtc acctgcaaga
     9301 tcctaaagca aggcctaacg gaattttctc cgcttctgtc cgtgccgggt ccaacgatta
     9361 ttacacaaga aatatttcaa gcgcatcaaa ctatcttact aatactttca actcatcaat
     9421 ttcttattca cgttcttttt tcaataaaca acttaacttt tcatctgcca tcacacattc
     9481 gcaaaacacg caaacacgaa tggtttattt aactgcccca agtataaacc ttagcatgaa
     9541 tacactgtat cctttccgca aaaaagagca ggagggcacg cttgcctggt atgaaaaaat
     9601 aagtgttggc tacagtagta cattacaaaa tcaattacaa aatcccgata gtctattctg
     9661 caaaaacacc acactgcagc agatgaaaaa cggaatgcaa cataacattc caatcagtgc
     9721 atcatttaaa gcgatgaaat attttaatct tacaccggcc attaatttta acgaacgttg
     9781 gtatattgaa acatacaagc aacgatttga tgctgacagc aacagaatta tgactgatac
     9841 ggttaaagga tttaacgctg cacatgagta taatggttca ttaagtctga acacaagaat
     9901 ttatggcatg aagcaattca agaaaggaaa aattgctgca ataagacatg tgctttcacc
     9961 aacactaagc tatatgtaca aaccggattt tggtgagaac aaatatggtt attacaaaac
    10021 cgtacaaaca gacagcattg gtaacagcag acgttattcc atctatgaaa acacactgta
    10081 tggtggtcct tcgcaaggca agtcaaacat tcttagtttt agcttagaca ataatctgga
    10141 aatgaaaacc agacaatata ctgatacggc agtgaacatt aaaaagataa aaattttaga
    10201 aagttttgga ctatcagcat catacaattt gtcggcagat tcatttaacc tctcgcctat
    10261 tggcatgaat gcacgaacaa ctctttttga tatggttagt ttaacagctt caggaacatt
    10321 tgacccctat ctgatgaacg atcagggaat acgaatagca aaatattctc ttacggaaaa
    10381 tggaaaaatt gcacgactca cctcagcaag cggaagtgtt ggctttaatc tgaaccgaca
    10441 gaaaggaaat aagaaaagtt taaaaggaac tgtcgaacag gtaaaagaaa ttaatgatca
    10501 tcctgatgat tatgttgact tttctgtacc atataattta tcggtcaatt acagcattgc
    10561 ctactcacga ccaactaatg aaaaaggtaa tctttcgcag atagttggtt tcaatggcga
    10621 catttcactg actgaacatt ggaaaataac atttaactcc ggttatgatt ttcaaaccaa
    10681 aaaacagtca tatacatcat taggttttta tcgcgatttg cattgctggg aaatgcgttt
    10741 aaactgggtg cctttcggat atcaggccaa ctattatttt caaatcaatg ttaagtcaag
    10801 tgtacttcag gatttgaagc tgacgaagaa aaacgaccgc tacgataatt tctaaaatta
    10861 ctcaacactg aatttatgtc cttcaatagc aagttctgta tttttaaata ccgttcttgc
    10921 ttcatcgagc aatggtttta aatcgcggta gcgtgcagaa aagtgaccta ctaaaagttt
    10981 gtcaacaccg gctttttttg caattgttgc agcttgcaga cttgttgaat gaaaagttgt
    11041 ttttgcacgt tctgccaagt cattcataaa tgtagcttca tgataaagca ggtttactcc
    11101 ttttatttca tcaataattg attcatcata acaagtgtcg ctacaaaaag cataagagcg
    11161 tggtgctgtt gacgaaattg tcaactctgt atttggaata atttttcctt cgtcatcctc
    11221 atagtcaaaa ccatctttca gtctgccata aaaagaaaaa ggaatattat gttgttgcag
    11281 ttttgagata attaactttc ttggctttgg tttttcagaa aataaatatc ctgtacatgg
    11341 aatacgatgg ttgagaacaa ttgatttgat gagcatatca tcgtcctcat aaataattgc
    11401 aggcgcataa tgctgcacag gatgaaaaat taactgatat cttaatctgg tttgcgacaa
    11461 tcgcaattga agttctataa catccattaa ctcgggctga ccatagatgt gaatttctgt
    11521 ggtgcgccct tgcagatgca tggttgaaag caaacctaca agacctaaat aatggtcgcc
    11581 atgcaggtgt gaaataaaaa tatgagaaag tttattgagt ttaaatttat atttcagaaa
    11641 ctgcccttgc gtgccttcgc cacaatcaat cataaaaaac cgctcccgga tattaagaac
    11701 ctgagcggtg ggatgacctt gatgtattgg cgttgctgaa ctgctgccta agatcgttaa
    11761 ttcaaaattc attacttgga aataaccatc cttcttgtaa tgttggctgt gcctgtatta
    11821 atagaataca tataaattcc tgacgacagg ttctctgcat tgatagtata agtattcaca
    11881 cctctttttg cagaaatagt ttcgctgaaa atatttttac caagtatatt gtaaatttta
    11941 aaatcaagtt ttgtgttaac aggtgaatta aaatatacaa cagatttgtt tttaaaagga
    12001 tttggcaggt tttgcgacat gctcaattta tcttttggca gcatttctat tactcctgaa
    12061 ttggtgtcaa tagtaatagt ataatatgtt accgacatgg gctggccgta agatatattt
    12121 ccaaggttag taatttttcc acgagctatg atatcaacag ttatcggata cacacctgct
    12181 gtaacaggcg tacctgaaag taacaaacat ccgttgctgc ttgccaaaaa ctgacacgtt
    12241 cctgggttgc atgcataagt taatccggga ggtaatcccg aaacacctaa aatggtaatt
    12301 gaatccaatt caaaatttgc attgaatggc acaccaccaa tattgattgt ggcagtagta
    12361 tcaaaaaatg ttcttacctg aataacctga ttgtaggcta cacccaccat gccattgctc
    12421 agacctgtag cactgtcggg ataaaccccg ggatcaatta ttgttgggtc aggagtacac
    12481 tgcgaaactg caatgcctga catagagata aacatcaatg agaagagtag cttttttatc
    12541 atttttcaga attttaaatt taacttacag aagcggcttt attgattaga gccaatgctt
    12601 catcaatttt tggtacaatg gttaatacag aatcaagttg agaaatgttg attagttttt
    12661 ttacaggatc gctgatttca gtaacaatga aattaccatt ggcattgcgg caaattctat
    12721 tgcctactaa aatagcactc agtcctgaag agtcacaata ctgtgtgtcg gaaagatcaa
    12781 gcacaatatg aataaagccc tgactgttga gtatcaacaa ttccgacttc agttctgcag
    12841 atataagtgt attaagcttt tgttcgtgaa gctttagcac tgcgtatttt tctttcttgt
    12901 ctaaggtata atgcatgagt attattataa aattggtgca aacaaatata aaaaagatat
    12961 tttaattaaa aacaaattgg tctttcgact tataaaatca acctaccggc tgaccggcta
    13021 atagataaag tactgccatg cgcacggcca cgccattttc gacctgatct aaaattatag
    13081 actgatcgga atccgcaact tcagtagtaa tttcaacccc acggttgatt ggtccgggat
    13141 gcatgatgac aatctttttc tgtaactttt tcagtatgtc gagggtgatg ccgtattgca
    13201 taatatactc tcttaaagag ggaaaatatt ttatatcctg cctttcaagc tgaatgcgca
    13261 atacattagc tacatcgcac cagttcaatg cattaatcaa gttggtttca actttcacac
    13321 ctagctgttc aatatatttt ggtatcaatg tagccgggcc gcaaaccatc acttctgcac
    13381 ccgatttttg cagacaataa atatttgaca atgccactct tgaatgtaaa atatcaccta
    13441 ctataactac ttttttgcct tttagtgttc ctaatttttc gcgtatagaa aatgcatcga
    13501 gtagtgcctg tgtagggtgt tcatgtgtac catcgcctgc attaacaata acagaattta
    13561 ctttctgcga taaaaacaaa gctgctccgg gttttggatg acgcatcact accaaatcaa
    13621 ccttcatggc aagaatatta tttaccgtat caattaatgt ttcgcctttg cttaccgatg
    13681 atgaagaagc tgtaaaattt agtatatcag cagagagtct tttctctgcc aactcgaatg
    13741 aaaggcgtgt gcgtgtagaa ttttcaaaaa acagattcac tactgtaaca tctcgcatgg
    13801 aaggcacttt ctttatcggg cgattgatga cttctttaaa agtttgtgcc gtatcaagaa
    13861 taaggttgat atcttcaggt ttcagatctt tgattccgat aagatgctta acgcttagct
    13921 ttcccattcg tttttgattt aatttcagaa actaaccaca cgccatcttt attgccgctc
    13981 tccttccact ctacctgcac tttatcagta atggttgtat caattgcctg ccctgtataa
    14041 tcaggttcaa tcggaaactg tctgctgaat tttctgtcaa ttaaaaccag tagctctacc
    14101 ttggcaggtc gcccaaaggc catgagtgca tccattgctg ctcttactgt tcgtccggta
    14161 aacaatacat cgtcaaccaa aacaaccttc ttgccctcaa caataaaatc tatctctgtg
    14221 cgcgatggaa caagcagctt ttcacttctg cgataatcgt ctctgaaaaa tgtaatatcc
    14281 aatgcaccat gaagaatact gctttccggc aaaaactccg aaagtctttt gcaaatacgc
    14341 ttagataatg gaactccctt cgactgaagt cctatgatac aggtatcttt aaaatttccg
    14401 tggttttcta tcaactgatg acaaaggcgc tctatagtaa tatctaactg tttgcccgca
    14461 agaattaaat gtgtcatcgt gtggcaaaaa tagcatttga aactaattgt aaaaaatcaa
    14521 attaccttgc cactcaaaaa tttacagtaa aattttgcaa tggagtaaat tacaataaag
    14581 ctatgccgat tattaaactg ttgccttttg catttttaat tgcggataca aatgctctat
    14641 ggcatctgcc aaaaaacatg gtataatggc atggtgtgtc agctcaattt tattggggtc
    14701 gctaagcgat tcaatcatat cataatattt ttcgctgcca taaagctcca taattttttc
    14761 tttcttatca gggcgatcaa aatagcgata cataccttct gcatcggctt ccggatgaaa
    14821 ctgcacgccc aatatttcat cactaaatc
//
LOCUS       DAOOOI010000052        14796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_808_length_14796_cov_72.354725, whole genome shotgun sequence.
ACCESSION   DAOOOI010000052 DAOOOI010000000
VERSION     DAOOOI010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14796
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_808_length_14796_cov_72.354725"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PKX59_07505"
     CDS             <1..1011
                     /locus_tag="PKX59_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90749.1"
                     /translation="SGIFNNSNYSNYTAPLSDRNLHLINTDVQTWSFYVFDSSQVDID
                     SCQLGEVGTQQSALVQANNLLLDGSGGYFWATDSSGILALGTTIYTMARSEKKGIFIL
                     AYGWQPFMAPTALSNSLMVCVQSTLGQDPVPYDGAVAWMQKISSPDTAIINTVVPING
                     SAWIDQGPSGGGMDFDKYSLYWSASTSNWIPIVTNVITEVHNNGLLGSWNTNGFTAGN
                     YFLRLMIKNNLGDSLEAIKPVYLKQLVSVNELSSIQSLIIYPNPAHDKISIEMSDIPE
                     SSEYNVEVLSATGSEVYTSTFANSTNHFNLDVSNLQQGLYYVLIKTNNGFLKKSKFVI
                     LK"
     gene            1249..3750
                     /locus_tag="PKX59_07510"
     CDS             1249..3750
                     /locus_tag="PKX59_07510"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOZ90750.1"
                     /translation="MIRAGIAIWAVMLCMLTANAQNVVTLAGQSGISGAVNGSGTSAT
                     FNNPHGMVCDVAGNLFVADRNNHLIRKITPAGVVSTFAGSGSAGSSDGIGTAASFFEP
                     WGIAIDQSGNLYVADTKNYLIRKITPTAVVTTVAGAGTSGVTDGPASNAQFGFPTGIA
                     VDASGNIYVSEFMTHVIRKISNGSVTTFAGTAFQSGDANGTGINAKFDHPHSIAIGQS
                     GNLFVTDTWNNKIRKITPAGVVSTYAGSTAGYVDGAASVSKFDNPLGICADASGDIFV
                     GDVNNFCIRKISNGTVNTFAGTAGTAGAANGPLLQASFNSPSCMAYHSSDKYFIGDEG
                     NELIRLINMNQSSNPLTLSTLNNVSVFCPSSTSVITASPQGLSSYTFKEGSTVLATNT
                     TGILSLGTLSAGTHLISCTATGLLGNYATTTALSITIVDASTPVISPSGPISICSGGS
                     ATLTASPGIAYLWTNGATTSSINATLPGTYAVSITYTGGCSYQSNSVTVNLNGSFSAS
                     ISPSAAQVICPGDSVKLTATAGTSYLWSNGKTTQSIYAKTAANYTVAVTNSFGCTSVS
                     PPVNVSLKSMPATIDASSDSSCSGEGVLLTVVQQNNTTYSWFDQATGGTALATGSSYA
                     TAPLAESTTFYVQLNSNGCTSISRFPVLAYIYPALHVDFTESQAVKADGGYAVSFQNN
                     TPTGNNTYTWNFGDGSANAISNEENPQHVYSSEGDYSVTLSVKDEYGCENSFTKNISV
                     TVAHDLFLPSAFTPNSDGINDIFRLKGSGFVSADMLILDQWGQLIFRTNNAVIGWDGS
                     VKGNLAPNGTYSYIVKIKLNDGNDKVLKGNISIIK"
     gene            3760..5250
                     /locus_tag="PKX59_07515"
     CDS             3760..5250
                     /locus_tag="PKX59_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOZ90751.1"
                     /translation="MKKIIIIVTLYMLSGIVNGQDPHISQYFNVPVFYNPALSGNGIQ
                     NMRIAVDYRNQWLAPGLSNKTQTIALDKIVNKVGFGLMVSKNSAGKDGFSRLSVLGGI
                     SYHTLFGYKKRHQFSSGFQVGILQKSFDPNKLTFESQYTPDIGYDPSASNGETFANTK
                     VIRPDINVGIAYHFAQGNKEILIKPFAGVSFSHINKPKESFIEEQNVNPIRMNFHGGA
                     SITCSKTIELKPLVYFMKQDGFQEMSAGMISELSLSNNSLVKLGLLYRNNDAMVAYAG
                     YQFNKIFLGTSYDFNISKYKPGGSGAYEISLVYTPQGRAKGKRNEERKPEPVNKKKLP
                     EQRVTPPQVLTPIPVIAPSIIEQNPIVVSLAEMPMKGKSSMKEKTELSNYNYQTIGYV
                     VFQDNSLKYDINNRFDVIEATIDYLYKNTNRKIIVSGNASEIEIKKNNSIGKQRAETV
                     KDYMIGKGIDADRILIADVKDKMPALMIEDGIQVERLSRADILLIK"
     gene            5260..6345
                     /locus_tag="PKX59_07520"
     CDS             5260..6345
                     /locus_tag="PKX59_07520"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOZ90752.1"
                     /translation="MNQIIQLLFPFISSLIIASLIIPSWIQLCSKWNLFDRPDTRKHH
                     TAIIPSLGGIGIFIAIMISIFLFGYNNQDSSFHSIAAAMLILFITGFFDDLTDLKARV
                     KLNIQLLAALIVTYSGLRIQTLNGFTGIHELPIIAQYGISIFIIIFLVNAYNFIDGLD
                     GLAATIGLIIFSIFTVLFHIHGQQAYAVLCVSVIGALVSFLYFNFNPARVFMGDTGSL
                     VVGFLIAICTLKLFNLWFTSPVVSFGPSLTIATIFILIFDLTRVVFIRVTNGISPFKA
                     DRSHLHHMICRQQFGHRGGTFIMAAFNILFIVLVLPLSKLRFITFLVFCFCVAILLMN
                     SKVMSYVALLRNKITGAPESKMGVDRG"
     gene            6460..7731
                     /gene="pabB"
                     /locus_tag="PKX59_07525"
     CDS             6460..7731
                     /gene="pabB"
                     /locus_tag="PKX59_07525"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOZ90753.1"
                     /translation="MSDTKFSPAEFEELLKSSNGFRNFCFLNSNNAAEGSYSGLTKYD
                     FLAGINALDTVEATSESLEKLETFIQKNKGKWMFGFMSYELKNEIEQLKSSHTDRLNF
                     PVLHFFVPQVVLVSEGGKLRMIDQLEQTSKEFVADDVELRQMNASANVSLKPRFSNDD
                     YIQAVEKLKRQLQQGNIYEVTFCQEFYNDAANINPFETYIKLNTNTRAPMSCFYKKEN
                     QFIISSSPERFMCRRGNKIYSQPIKGTAPRSASREEDEWLKDNLQNSEKERAENLMIV
                     DLVRNDLSRIAKPGTVEVNELLSVYTFKTVHQLISTVSCEVEPKINFSEIIRAAFPMG
                     SMTGAPKISAMTLIDEHEKTNRGLFSGSVGYIDPSGDFDFNVIIRTILYNAQRNYVSV
                     QTGGAITIQSDPAAEYEECLLKAKAMMDVLG"
     gene            7760..9109
                     /gene="tilS"
                     /locus_tag="PKX59_07530"
     CDS             7760..9109
                     /gene="tilS"
                     /locus_tag="PKX59_07530"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOZ90754.1"
                     /translation="MLLQFQQFVKEKKLFSKEQTILVAVSGGIDSMVLLQLLLQSGYK
                     IAVAHCNFKLRGEESDGDAQFVHSFCLENKIVIHSTEFETEKLAKQNQQSIQGTAREL
                     RYNFFQELCSSFHYDFIATAHHADDVAETVLINLSRGGGLAAWHGIEVKRENIVRPLL
                     FAQKHEIEEYAKQNKIQWRTDSSNQKEDYTRNLIRHQIIPVLKKINPSVVQTLFHHAE
                     IVADAEQIIHEHIEKFRAASVVTDENKTIVPISDLLNTSAAQTLLFYILQPYGFNATH
                     TVDIFKSLNHSESKTFYSDSFQLVKEREQLVIIPFEKKRISKIYFEIPTTGTFSIADS
                     QISVEVLENTEDLYHIILKNNNQVIGYFDADKIEFPIIVRTWQHGDYFFPLGMHGKKL
                     VSDFFTDEKMSQSEKQKQLLFLSSNEVIWLEGRRISEKFKVEKNSKSVLKISVKAIV"
     gene            9211..11577
                     /locus_tag="PKX59_07535"
     CDS             9211..11577
                     /locus_tag="PKX59_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOZ90755.1"
                     /translation="MKSIITIVLLVLINANAFAQSEKYQKVRIWLEGKTVSSLAQTGV
                     DLSETHYRKGLYFETDLSTSELNSVSNAGFRTEVLIDDVKQFYKQRNEQQNQKTSAVM
                     SCSSAPDFPQPAHFGYGSMGGFFTYQELLNNLDSMASLYPNLITVKQPISNTLTTVEG
                     RPIFYVKISDNPNLTEPEPEVLYTALHHCREPNGMAAVIYYMWYLLENYATNTDIQQL
                     VNNTEMYFVPCVNPDGYVFNEMTDPNGGGMWRKNRQQFPGGEYGVDLNRNYGVQWGYD
                     DVGSSPDPADDTYRGPAAFSENETQMISAFCQQHNFKLALNYHTYSNLLIYPWGYIPD
                     YYTPDSALYKNYGDILTTYNKYAAGTANQTVGYMVNGSSDDWMYGEQTLKPKTFAMTP
                     EIGSADDGFWPQQSRIVDLCKENMYANVMLARLAGKYGQATDKSKWNIPALTSYIPFD
                     FKLLGLDTVGTFTVSLTPLSANIQTTGAAKTFSGLNFNLSVTDSITITLNSTTLPGDE
                     VKFLLTVNNGLYDISDTITKYYGPVVTPFFENGNTTSAWNTQFWGTATSSFISALSSI
                     ADSPIGDYQSSSINEITTISNINLLNAAAARLTFFTKWATEPQYDYAQLQISDNNGST
                     WTPLCGKYTVPGSPFQDVGDPVYQGVNLNWVKEDINLNAYTGKNVKLKFIMVSDGFVE
                     YDGIYVDDLSVEKIVASGVGINNLENNTFNIYPNPATDIARIETAANAKNINVINTLG
                     QTVFTENLILEQHHYDLNIADFAAGVYVVKVKYSSGAVAQKRLMVYKK"
     gene            complement(11682..14333)
                     /locus_tag="PKX59_07540"
     CDS             complement(11682..14333)
                     /locus_tag="PKX59_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90756.1"
                     /translation="MKTLALLFILLTATVQAQEIEWQNTIGGNYTDQLYSIQQTTDGG
                     YILGGWSESYISGDKTENSFGGSDYWVVKLDSIGNIQWQNTIGGNSGDGLYSVQQTTD
                     GGYILGGVSTSNISGDKTENSQGYIDYWVVKLDASGNIQWQNTIGGNSDDYLTSIQQT
                     TDGGYILGGWSNSNISGDKTENSQGDYDYWVLKLDPTGNIQWQNTIGGSINDQLYSIQ
                     QTIDGGYILGGWSNSNISGDKTENSQGTDYWVVKLDASGNIQWQNTIGGSFLDYLNCM
                     QQTSDGGFILAGSSQSGISGDKTEDSQGGSDYWVVKLDAFGNIQWQNSIGGNSDDYLT
                     SIQQTIDGGYILGGYSNSGISGDKTENYQGLYDYWVVKLDATGNIQWQNTIGGNSWDE
                     LISIQQTNDGGYILGGHSSSGISGDKTENSQGDYDYWVVKLTNKISLINGKIYIDANS
                     NTIQDSNEINLPNRMITEQNTGRIAFSEQNGNYNVSVLDTGNFTVSAATLNYYNSVPL
                     VHTAYFNSIQQVDSLNDFAFQPNGIINDVCVKLTPVSLFRSGFDATYNISFVNNGTTT
                     LTPTVIFFPDTSVSFVTSSATPASVTTDSIVWNIGALTPFQSGNIVVTVNVHTGLPIG
                     SLINSSVRIEPVAGDANPGCNYSSWEVFTIGSFDPNDILVSRDTLFTTEIPNPPYLDY
                     IIRFQNTGNDTAFNVKVLNPLDTNKLQLNTLEYVASSHPVNMNFIYHERNMEFKFDNI
                     LLPDSNVNESLSHGFIHYRIKPKTSLVLNDNIQNNAAIYFDFNDPVITNTATTHVVLP
                     TGIASYQSPENIQLFPNPAQSEINLQHITAVEKIQVIDVLGKIVFEKNTNNQQYLKIN
                     ISQVQNGVYFVKAYSAQGVVVKKFVKR"
     gene            complement(14566..>14796)
                     /locus_tag="PKX59_07545"
     CDS             complement(14566..>14796)
                     /locus_tag="PKX59_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90757.1"
                     /translation="ENIQLFPNPAQSEINLQHTTAVEKIQVIDVLGKILFEKKTNSQQ
                     NLKINISQFQNGVYFVKAYSTQGVVVKKFVKR"
BASE COUNT         4859 a         2482 c         2734 g         4721 t
ORIGIN      
        1 tcgggcattt ttaataattc aaattatagc aattatactg caccattatc ggacagaaat
       61 ttacatttaa ttaatacaga tgtacagaca tggagttttt atgtttttga ttcatcacag
      121 gttgatatag acagttgtca gttaggtgaa gtgggcacac agcaaagtgc acttgtgcag
      181 gcaaacaatt tattacttga tggaagtggc ggatattttt gggcaacaga cagcagtggc
      241 attcttgctt taggcacaac tatttatact atggcaagaa gtgagaagaa aggaattttt
      301 attcttgctt atggctggca accttttatg gcaccaactg cattaagcaa cagtttgatg
      361 gtttgtgttc aaagcacttt aggacaagac cctgttccgt atgatggagc agttgcatgg
      421 atgcaaaaga tttcatcacc tgatacagct ataatcaata ctgttgttcc gattaatgga
      481 tcggcttgga ttgaccaagg accatcaggt ggaggaatgg actttgataa atacagtttg
      541 tattggagtg ccagtacttc aaattggata ccaattgtta caaatgtaat cactgaagtt
      601 cacaacaatg gattactcgg aagttggaat accaatggat ttactgcggg aaattatttt
      661 ttgcgtttga tgataaagaa taatttgggt gacagccttg aagcaattaa acctgtttac
      721 ttaaagcaat tagtttcagt taacgaacta tcatctatac aatcattaat tatttatcct
      781 aacccagcgc atgacaaaat tagtattgaa atgagtgaca ttccagagag tagtgaatac
      841 aatgttgaag tgttaagtgc aaccggtagt gaagtttata caagcacatt tgccaattca
      901 accaatcatt ttaatcttga tgttagcaat ttacaacaag gattatatta tgtccttatc
      961 aaaacaaata acggtttttt aaaaaaatcg aagtttgtta ttttgaaata gtatgaattg
     1021 attttatttt tcgatcattt tatatacaga ttcatcaaaa tacatttcac ataagtataa
     1081 agttacattg acaatttagt caaacagttg atcttgtccg taaattgttg tttcagagtt
     1141 cctaatcaca ttttcaaaga gtaatttttt tctgatgtaa cgcttcaatg gtgtaacctt
     1201 actttaaata gaaagtataa aagggagttc acttttgaag aaaaacgaat gataagagcc
     1261 ggaatagcaa tatgggctgt catgttatgc atgttaacag ccaatgctca gaatgtagtt
     1321 acactggcag ggcagagtgg catttcaggt gctgtcaatg gctcgggtac ttctgcaacg
     1381 ttcaacaatc cacatggaat ggtttgcgat gtggcaggaa acctatttgt tgcagacaga
     1441 aataatcacc ttatcagaaa aataacacct gcaggagtag tatcaacatt tgcaggatct
     1501 ggtagtgcag gaagttcaga tgggatagga acagcagcat cattttttga accttgggga
     1561 attgccattg atcagtcggg aaacctttat gttgccgaca caaaaaatta tttaataaga
     1621 aaaattacgc caacagcagt tgttacaaca gttgccggag cgggaacttc aggcgttact
     1681 gacgggccgg caagcaatgc acagtttgga tttccaacag gtattgcagt agatgcttca
     1741 ggaaatattt atgtgagtga gtttatgaca catgttatcc gcaagataag taacggtagt
     1801 gttaccactt ttgcaggaac agcttttcag agtggcgatg ccaatggtac cggaattaat
     1861 gcaaagtttg atcatcctca tagtattgct attgggcagt caggaaattt atttgttaca
     1921 gatacctgga ataataaaat cagaaaaatt actcctgcag gtgtagtgag cacctatgca
     1981 ggaagtactg caggatatgt tgatggtgcc gcttctgtga gtaaatttga taaccctttg
     2041 ggcatttgcg cagatgcatc aggcgatata tttgttggtg atgtaaataa tttctgtatc
     2101 cgtaaaatca gcaatggcac agtaaacact tttgcaggaa cagcaggaac agcaggcgcg
     2161 gctaatggcc ctttgcttca ggcctcattc aattcaccat catgtatggc atatcattca
     2221 tcagacaaat attttattgg tgatgaagga aatgaattga tccgactcat caacatgaat
     2281 cagagtagta atccactaac actttcaaca ttaaataatg tgagtgtatt ttgtccaagt
     2341 tcaacttctg ttattacagc ttccccacaa ggactaagca gctatacttt taaagaagga
     2401 agtacagtat tggcaacaaa tacaacaggg atattgtctt taggaacatt atcagcaggt
     2461 acacatctta tcagttgtac tgcaactgga ctattaggaa actatgcaac aacaacagca
     2521 ttaagtatta caattgttga tgcaagcaca cctgtaattt ctccttcggg gccaatatca
     2581 atttgctcag gtggaagtgc tacattgaca gcttcacccg gtatagcata cctgtggaca
     2641 aatggagcaa caacatcttc aatcaatgcc accctgccag gcacttacgc agtgagcata
     2701 acttatacag gtggctgcag ttatcaaagt aattcagtta ctgtaaactt aaacggatca
     2761 ttcagtgcat ctatatctcc atcagcagca caggttattt gccccggtga ctcggtaaaa
     2821 ttaacagcta ctgcaggaac atcatacttg tggtctaatg gaaaaacaac acaaagcatt
     2881 tatgctaaga cagccgcaaa ctacaccgta gctgtgacaa acagttttgg atgtacttct
     2941 gtttctccac cagtaaatgt ttcgttgaaa tctatgccgg caacaattga cgcaagcagc
     3001 gattcatcat gcagcggtga aggtgttttg ttaacagtag ttcagcaaaa caacacaacc
     3061 tatagttggt tcgatcaggc aacaggaggc acagcacttg caacaggcag cagctatgct
     3121 actgcacctc ttgcagaaag caccacattt tatgttcaac taaattctaa tggctgcaca
     3181 agcatttcaa gatttcctgt acttgcatac atctatcctg ctttacatgt tgattttaca
     3241 gaaagccagg ctgtgaaagc agatggtggt tatgctgtat catttcaaaa caatacacca
     3301 acagggaata acacctatac ctggaatttt ggtgatggtt ctgccaatgc gatttctaat
     3361 gaagaaaacc cacaacatgt ttactcatct gaaggggatt acagtgtaac actatcagtt
     3421 aaggatgaat atggatgtga gaacagtttc acaaaaaata tttctgttac cgttgcacat
     3481 gacttgtttt taccctctgc ttttacacct aatagcgatg gcatcaatga tattttcaga
     3541 ctaaaaggtt ctggatttgt ttcagctgac atgttgatac ttgatcaatg gggacagtta
     3601 attttcagaa caaacaatgc agtaatagga tgggatggct ctgtaaaggg aaatcttgca
     3661 cccaatggaa catatagtta catagtaaag ataaagctga atgatggcaa tgataaagtg
     3721 ttgaagggca atatttcaat catcaaataa ccggcagaca tgaaaaaaat aattatcata
     3781 gttacacttt atatgttgtc gggaattgtt aatggacagg atcctcacat atcacaatat
     3841 tttaatgtac ctgtatttta caatcctgct ttatcgggta atggtattca gaatatgcgc
     3901 attgcagttg attatcgcaa tcagtggctt gctcccggat tgagtaacaa aacacaaaca
     3961 atagcattag ataaaattgt caacaaggtg ggttttggtt taatggttag taaaaacagt
     4021 gcaggtaaag atggctttag cagattaagt gttctgggag gcataagtta tcatacacta
     4081 tttggctata aaaaaagaca tcagttttcg tcaggatttc aggttggaat tcttcaaaaa
     4141 tctttcgacc ccaacaaact tacatttgaa agtcagtaca ctcctgatat tggatatgat
     4201 ccttcagcat caaatggaga aacatttgcg aacacaaaag ttatacgccc tgatattaat
     4261 gtaggtatag cctatcattt tgcacaaggc aataaagaaa ttctgattaa accatttgcc
     4321 ggtgtttcat tttctcatat caataaacct aaagaatcat ttattgaaga acagaatgta
     4381 aatcctataa gaatgaattt tcatggaggt gcatcaatta cttgttcaaa aactattgag
     4441 ctgaaacctc ttgtttattt tatgaagcaa gatggctttc aggaaatgag tgctggtatg
     4501 atttctgaat tgtcattaag caataacagt ttagtaaaat taggattgct ttacagaaat
     4561 aacgatgcta tggttgctta tgcgggctac cagtttaata aaatatttct tggaacaagc
     4621 tatgacttta atatttcgaa gtacaagccg ggaggtagtg gcgcttatga aatttcactc
     4681 gtttacacgc cacaaggtcg tgcaaaaggt aaacgtaatg aagaaagaaa acctgagcct
     4741 gtcaataaga aaaaattgcc tgagcaaaga gttactcctc cacaagtgct gacacctata
     4801 cctgtaattg ctccttcaat aattgaacag aatcctatag ttgtttcact tgcagaaatg
     4861 ccaatgaaag gtaaaagcag tatgaaagag aaaacagaac tttctaatta caactatcaa
     4921 acaatagggt atgtagtgtt tcaggataat agtttgaagt atgatatcaa taaccgcttt
     4981 gatgtaatag aagctacaat tgattatttg tataaaaaca caaacagaaa aattattgtt
     5041 tcaggaaatg cttcggaaat tgaaataaag aaaaataaca gtataggaaa acaaagagca
     5101 gaaacggtta aagattatat gataggtaaa ggaattgatg ctgacagaat cctgattgct
     5161 gatgttaaag acaaaatgcc tgcattaatg atagaagacg gaatccaagt agaaagacta
     5221 tcgcgagcag atatattatt gattaaataa accacctgta tgaatcagat tattcaactg
     5281 ttatttccat ttatttcttc tcttattatt gcatcactca tcattccatc gtggattcag
     5341 ctttgttcta aatggaattt gtttgaccgc cccgacactc gcaaacatca tacagcaatt
     5401 atcccttcgt taggaggtat cggaattttt attgctataa tgatttcgat ctttttgttt
     5461 ggatacaaca atcaggattc aagttttcat tccattgcag cggcaatgct cattttattt
     5521 ataaccggat tttttgatga tcttacggat ttaaaagcaa gagtaaaatt aaatatacaa
     5581 ctcttggcag ctttaatagt cacctattca ggattacgca ttcaaacatt aaatggtttt
     5641 acaggcattc atgaattacc tatcatagct caatatggca ttagtatttt tattataata
     5701 tttttagtaa acgcctataa ttttattgat ggattagatg gcttggcagc aacaataggg
     5761 cttattatat tcagcatttt tactgtattg tttcatattc atggacaaca ggcttatgca
     5821 gttttatgtg tcagtgttat tggtgctttg gtatcttttc tgtattttaa ttttaatcct
     5881 gcaagagtat ttatgggcga tacgggttcg ttagttgtag gttttcttat tgccatttgc
     5941 actttaaaac tttttaatct ttggtttaca tctcccgtag tttcttttgg cccttcgtta
     6001 accattgcaa caatttttat actaatattt gatttaacaa gagttgtttt tatcagggta
     6061 acaaatggta tttcaccgtt taaagcagac agaagccatt tacatcacat gatttgccgt
     6121 cagcaattcg gacatcgtgg aggcactttt attatggctg cattcaacat tttatttatt
     6181 gttttggttc ttccgttaag taaactgcgg tttatcacat tccttgtttt ttgtttctgt
     6241 gtagctattc tacttatgaa ctctaaggtc atgtcgtatg ttgcactatt aagaaataaa
     6301 attaccggag cacccgagag taaaatgggt gttgacagag ggtaattatc ggtgaataat
     6361 acttataaaa catttcaatt tattgaactt gttaaaaacg tgttcactac ttttttagaa
     6421 tcataaacaa cagaaatctt ttttgatttt ttttgcttta tgagcgacac taaattttca
     6481 cctgccgaat ttgaagagct gttaaagtca tcaaatggtt ttcgtaactt ttgtttttta
     6541 aacagcaaca atgctgcaga aggtagctat agtggcttga caaaatatga ttttcttgca
     6601 ggcattaatg cgctcgatac agttgaggca acatcagaat ctttagagaa gctggagaca
     6661 tttattcaga agaataaagg caagtggatg tttggtttta tgtcgtatga gctaaaaaat
     6721 gaaatcgaac aactaaaatc atcgcataca gaccgattga attttcctgt tttacatttt
     6781 tttgtaccac aagttgtttt agtttctgaa ggtggaaaac ttcgtatgat tgatcagctt
     6841 gaacagacat cgaaagaatt tgttgccgat gatgtagagc ttagacaaat gaatgcatct
     6901 gctaatgttt ctttaaagcc tcgtttcagc aatgatgatt acatacaagc tgttgaaaaa
     6961 ttaaagagac agttacagca gggaaatatt tatgaagtaa ctttttgtca ggagttttat
     7021 aatgatgcag caaacatcaa tccgtttgaa acctatataa aacttaatac aaacacacgt
     7081 gctcccatgt cgtgttttta taaaaaagaa aatcaattta ttatttcatc aagtcctgag
     7141 cggtttatgt gtagaagggg aaataaaatt tattcgcagc caataaaggg aacagcacca
     7201 cgcagcgcat cacgtgagga agatgaatgg cttaaagata atctgcagaa tagtgaaaaa
     7261 gaacgtgcag aaaatttgat gattgttgat ttggtacgaa atgacctttc aagaattgct
     7321 aagcccggca cagttgaagt gaatgagttg cttagtgtct atacgtttaa aacagtacat
     7381 caattgattt ctacagtaag ctgtgaggtt gaacccaaaa ttaatttttc tgaaatcata
     7441 cgtgctgcat ttcctatggg aagtatgaca ggtgctccta aaataagcgc tatgacattg
     7501 attgatgaac atgagaaaac aaacagaggc ttgttttccg ggagtgttgg ttatattgat
     7561 ccttcaggcg attttgactt caatgttatt atccgtacta tattgtataa tgcacaacgt
     7621 aactatgttt cagtgcagac gggcggagca ataaccattc aatctgaccc tgctgccgag
     7681 tatgaagagt gtttgttaaa agctaaagca atgatggatg ttttaggatg atgtacatta
     7741 gcagaaaata aagcaacact tgttattaca gtttcaacaa tttgtaaaag agaaaaaact
     7801 cttcagtaaa gagcaaacaa ttcttgtagc tgttagtgga ggcattgatt caatggtttt
     7861 attgcaacta ctgctacagt caggatataa aattgctgtt gcacattgta attttaaatt
     7921 gcgtggtgaa gaatctgatg gtgatgcaca gtttgttcat tcattttgtt tggaaaacaa
     7981 aattgtgatt cattctactg aatttgaaac agaaaagttg gcaaaacaaa atcaacaatc
     8041 cattcagggt acagcacgtg aacttagata taattttttt caggagttat gtagtagctt
     8101 tcattatgat tttattgcta cggctcatca tgccgatgat gttgcagaaa ctgtattaat
     8161 taatttgagt cggggtggtg gtttggctgc atggcatggt attgaagtaa agagagaaaa
     8221 tattgtcagg cctttattgt ttgcacaaaa acatgagatt gaagaatacg ctaaacagaa
     8281 caaaattcaa tggcgcactg acagctcaaa tcagaaagaa gactatacaa gaaatctcat
     8341 cagacatcag attattcctg tattaaagaa gataaatcct tctgttgtac agacattgtt
     8401 tcatcatgca gaaatagtag cagatgccga acaaattatt catgagcata ttgaaaaatt
     8461 cagagcagca tctgtagtta ctgatgaaaa taaaactatt gttccgattt cagatttgtt
     8521 aaatacatca gcagcacaaa ccttattgtt ttatattctt cagccgtatg gatttaatgc
     8581 aacacataca gttgatattt ttaaatcctt aaatcattct gaaagtaaga ctttttattc
     8641 tgacagtttt caattggtaa aagagcgaga acaacttgtt ataataccat ttgaaaagaa
     8701 gaggattagt aaaatctatt ttgaaattcc aacaacagga accttttcta ttgctgattc
     8761 tcaaataagt gtagaagttt tggagaatac agaggacttg tatcatataa tattaaagaa
     8821 caacaaccaa gtaatcggtt attttgatgc agataaaata gaatttccaa ttattgtaag
     8881 gacatggcag catggcgatt atttttttcc attaggtatg catggcaaaa agttagtatc
     8941 agattttttt actgatgaaa aaatgagtca gtcggaaaag caaaaacaat tgttgttttt
     9001 aagtagtaat gaagtgatat ggcttgaggg cagacgtata agtgaaaaat ttaaagtgga
     9061 gaaaaacagt aagtcggttt taaaaattag tgttaaggct atagtttaaa acaaacaggt
     9121 taaaattact ttgagtaaag atttttttag atagttttgt tagtgtaaag gccatatcaa
     9181 aaaagcaata taaaatctta ttcaattaaa atgaaatcaa taattacaat tgtgttgtta
     9241 gtgttaatca atgctaacgc atttgctcag tcagagaaat atcagaaagt acgaatctgg
     9301 cttgaaggga agactgtttc atcacttgca caaacaggag tagatcttag tgagacacat
     9361 tatcgcaagg gactttattt tgaaacagat ttaagtactt ctgaattaaa tagtgtcagc
     9421 aatgccggct ttagaacaga agtattaatt gatgatgtaa aacaatttta taagcaacgt
     9481 aatgaacagc aaaatcagaa aacatcagca gtaatgtcat gcagctcagc acctgatttt
     9541 ccgcaacctg cacactttgg ttatggtagc atgggcggat tttttactta tcaggagttg
     9601 ttaaacaatc ttgattcaat ggcatcgctt tatcctaatt taatcactgt aaaacaacca
     9661 ataagcaata cgttaacaac ggttgaaggt cgtcctattt tttatgtaaa aatttctgat
     9721 aaccccaatt taaccgagcc tgaacctgaa gtactttaca cagcattgca tcattgtcgc
     9781 gagcctaatg gtatggcagc agtgatatat tacatgtggt atctgctcga aaactatgct
     9841 accaataccg atattcagca gttagtaaat aatactgaaa tgtattttgt gccctgtgtt
     9901 aacccggatg gatatgtgtt taatgaaatg accgatccca atggtggggg tatgtggcga
     9961 aaaaacagac aacaatttcc gggtggagaa tatggtgttg atcttaatcg taattatggt
    10021 gttcagtggg gttatgatga tgttggttca tcgcccgacc ctgcagatga tacttatcga
    10081 ggccctgcgg ctttcagtga gaatgaaaca caaatgataa gtgctttttg ccaacaacat
    10141 aattttaagc tggcattaaa ttatcataca tatagtaatt tattgattta tccatgggga
    10201 tatattcctg actattatac acctgacagt gctttgtata aaaactatgg tgatatttta
    10261 acaacatata ataaatatgc agcaggtact gcaaaccaaa cagtaggtta tatggttaat
    10321 ggaagttctg atgactggat gtatggcgaa caaacattaa agccaaaaac atttgcaatg
    10381 acaccagaga ttggtagtgc agatgatggc ttctggccgc aacaatcacg cattgttgat
    10441 ttgtgcaaag aaaatatgta tgccaatgtt atgcttgcac gtctggcagg taaatacggt
    10501 caggctactg ataaaagcaa gtggaatatt cctgcactta cttcatatat accattcgat
    10561 tttaaattac ttggattaga tactgttgga acatttactg tttcgttaac gccactgtct
    10621 gctaacatcc aaacaacagg agcagcaaaa acattcagtg gtttaaattt taatctatcg
    10681 gttacggatt caataaccat cacattaaat tcaacaacgt tacccggtga tgaagtaaaa
    10741 ttcctgttaa ccgtaaacaa tggcttatat gatatttccg ataccattac aaaatattat
    10801 ggccctgttg tgacaccgtt tttcgaaaat ggaaatacca catcagcatg gaatacacaa
    10861 ttctggggaa cagctacatc gtcttttatc tcggcactat caagcatagc agactctcct
    10921 attggcgatt atcaatcatc atcaattaat gaaatcacaa ctatcagtaa tattaatttg
    10981 ttgaatgctg ctgctgcacg tttgacattt tttacaaagt gggcaacaga accgcagtat
    11041 gattatgcac agttgcaaat ttccgataac aatggtagca catggacacc actttgtgga
    11101 aaatatacag taccgggaag tccttttcag gatgtaggtg atcctgttta tcagggtgtg
    11161 aatttaaatt gggtaaaaga agatattaat ctgaatgcct atacaggaaa aaatgtgaag
    11221 ttgaaattta ttatggtaag tgatggcttt gttgaatatg atggtattta tgtagatgat
    11281 ttaagtgtag aaaaaattgt agcttcaggt gttggaataa acaatcttga aaacaacaca
    11341 ttcaatattt accctaatcc tgcaacagat attgcaagaa ttgaaactgc agctaatgca
    11401 aaaaatatta atgttatcaa tactttggga caaacagtat ttactgaaaa cttaatttta
    11461 gaacaacatc attacgattt aaacattgct gattttgctg caggtgttta tgttgttaaa
    11521 gtaaaatata gcagtggtgc tgttgcacaa aaacgattga tggtttataa aaagtagaat
    11581 atgaattgat gtgactccaa caagtcattg cagatacaaa ttcaacaaat ctcctcagcc
    11641 gcggctgagc cacggctgag cgatggctaa tctaaaattg cctatctctt aacaaacttc
    11701 tttaccacca caccttgtgc gctgtatgcc tttacgaaat aaactccgtt ttgaacttga
    11761 ctgatgttta tctttaaata ttgttggttg ttggtattct tttcaaacac aatttttcca
    11821 agtacatcaa tcacttgtat tttttcaacg gctgttatgt gttgaagatt tatttcagac
    11881 tgtgcaggat ttgggaataa ttgtatgttt tctggactct gatatgatgc tattcctgtt
    11941 ggtagtacaa catgcgttgt tgctgtgttg gtaattacag ggtcattaaa atcaaaataa
    12001 attgcagcat tgttttgtat gttatcattc aataccaaac ttgttttagg ttttattctg
    12061 taatgaataa agccatggct taacgactcg ttaacattac tatcgggcaa aagaatatta
    12121 tcaaacttaa attccatgtt gcgctcatga tagataaaat tcatgtttac aggatgcgat
    12181 gatgcaacat attctaaggt gtttaactga agtttatttg tatctaatgg atttaaaact
    12241 ttaacattaa atgctgtatc gtttccggtg ttttgaaaac ggatgatgta atctaaatag
    12301 ggtggattgg gaatttctgt agtgaataaa gtatctctac ttactaaaat atcattgggg
    12361 tcaaatgaac ctatggtaaa aacctcccaa gaactgtaat tacaaccggg attagcatca
    12421 ccggcaacag gctctattct tacacttgaa ttgataagtg aaccaatggg caaacccgta
    12481 tgtacattta ctgtaacaac aatattacct gactgaaaag gtgtaagtgc gccaatattc
    12541 caaactatag aatcggttgt aacagatgca ggtgttgcac ttgaggttac gaaagataca
    12601 cttgtatcag gaaagaaaat tacagttggc gtaagtgttg tagtgccatt atttacaaag
    12661 ctgatattat aagttgcatc aaacccagaa cggaataaag aaactggtgt aagcttaaca
    12721 cacacatcat ttataatacc attaggctga aatgcaaaat catttaatga atctacttgt
    12781 tgaatagaat taaaataagc agtatgaact aatgggacac tgttgtagta atttaatgtt
    12841 gcagcagaaa cagtaaaatt tcctgtatct aaaacagata cattataatt cccattttgc
    12901 tctgaaaatg caatacgacc tgtattttgt tcagtaatca ttctgttagg taaattgatt
    12961 tcatttgaat cttgtattgt gttactattg gcatcaatat atattttacc atttatcaga
    13021 ctaattttat ttgttagttt cactacccag taatcataat ctccttgaga attttcagtt
    13081 ttatcaccgg aaataccgga gctagaatgc cccccaagaa tataaccacc atcattagtt
    13141 tgttggatag aaattaattc atcccaacta tttccaccaa tagtattttg ccattgaata
    13201 ttacctgttg catctaactt caccacccag taatcataaa gtccttgata attttcagtt
    13261 ttatccccgg aaataccgga attagaatac cccccaagaa tgtatccacc atctattgtt
    13321 tgttgtatag aagttaaata atcatcacta tttccaccaa tagaattctg ccattgaata
    13381 tttccaaatg catctaattt aaccacccag taatcactac ctccttgtga gtcttctgtc
    13441 ttatcacctg aaataccaga ctgtgaagat cctgcaagaa taaaacctcc atctgaagtt
    13501 tgttgcatgc aatttaaata atcgaggaag ctacctccaa tagtattctg ccattgaata
    13561 tttccagatg catctaattt aaccacccaa tagtctgtcc cttgtgaatt ttcagtctta
    13621 tctcctgaaa tgttggaatt agaccatcca ccaagaatat atccgccatc tatagtttgt
    13681 tgaatggaat atagttgatc atttatactt ccaccaatag tattttgcca ttgaatattt
    13741 cctgttggat ccaatttcaa aacccagtag tcatagtctc cttgagagtt ttcagtctta
    13801 tctcctgaaa tgtttgaatt agaccacccc ccaagaatat atccaccatc tgttgtttgt
    13861 tgtatagaag ttaaataatc atcactattt ccaccaatag tattctgcca ttgaatattt
    13921 ccagatgcat ctaatttaac cacccagtaa tcaatatatc cttgcgaatt ttcagtttta
    13981 tccccggaaa tgttagaagt agaaactcca ccaagaatat atccgccatc tgtagtttgt
    14041 tgaaccgaat ataacccatc tccactattt ccaccaatag tattttgcca ttgaatgttt
    14101 cctatactat ctaactttac tacccaataa tctgaacctc caaaagagtt ttctgtctta
    14161 tcaccggaaa tataagattc tgaccatccc ccaagaatgt atccgccatc tgtagtttgt
    14221 tgtatggaat atagttgatc agtataattc cctccaattg tattctgcca ttctatttcc
    14281 tgtgcttgca cagtagcggt aagtaaaata aataacaatg ctaaagtttt catgatgctt
    14341 atattttgtt tattcaaaag tatgtaattc aaacaagata aaaaacaatt taggtaattt
    14401 ttatcaaggc aagaggatgg atagaaactg tctgatgtag aaattaattc aactaatcta
    14461 ttataatcac ggtttccatt taatataaaa tcagactact ggctaaaatc ttcaaatcta
    14521 aaaatcccct caaccgtggc tgagccgtgg ctgatcaaaa atttactacc gcttaacaaa
    14581 cttctttacc accacacctt gtgtgctgta tgctttcaca aaataaactc cattttgaaa
    14641 ttgactgatg tttatcttta aattttgttg gctgttggtt ttcttttcaa acaaaatttt
    14701 tccaagtaca tcaatcactt gtattttttc aactgctgtt gtgtgttgaa gatttatttc
    14761 agactgtgca ggatttggga acaattgtat gttttc
//
LOCUS       DAOOOI010000053        14251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_849_length_14251_cov_18.016343, whole genome shotgun sequence.
ACCESSION   DAOOOI010000053 DAOOOI010000000
VERSION     DAOOOI010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14251
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_849_length_14251_cov_18.016343"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2724
                     /locus_tag="PKX59_07550"
     CDS             <1..2724
                     /locus_tag="PKX59_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90758.1"
                     /translation="QITSTTDDISCFGISTGSDGKYVLKGVSTSPKEKTVWACKPGAT
                     PNIISQNKKITLSQQNELDFNLISDNELIIENIFGFEVDIQSKVKQSDGIWLVSGNLI
                     NLPANDNFSLHDSKQKISFTDLKIKKSDATKNGIPVGVSVENTFSTDLTNIKLLLQNS
                     FGVIQKPSSGDVLQVKSDNQKGRLTGKIAVQKSSFKFTQSYITFNNDPNEAMLITDKP
                     GSFITDISSIDIATSTKKKFGLANLQGKTMKFSLLGFDAEADAINSWLQDNSINLQTI
                     IHINALPDMTPSTLDINAGNLIIHPEKLEPLKGNQPIKFNLEKWQFIGNNWQLSQGDN
                     SINIATGTIKTGSIDIPISDISLQPDHIGIGDFDVNNLSFGGIIPVHVTSTNPVFGRN
                     KSIGSDQKPHYEFRLIGENGSPGVVIKTLPGMKTGEEMKFQNLSLISNGEQIINPGNQ
                     PNSITFYSVMKVKPLSFMSGQDYVNMTCGIDLGIPQLKETSGIIQFSKEAGQIKFQLY
                     PLNVSLKGPGDVDFVANVQFNDRPQNLTEGKFTALGTIYEKEGMVLKAILNKTTTAAW
                     IQVDPENQKLPLGGSTTSLANIKGKMEADMSSGIWKNFTFSGEMQGFKGMQGDTRKTF
                     VVTGSINASNQNIEVKNIPSGFGNIGLNYDFVNSRFTGNLQLDKNIGPLALAGTANLL
                     VDPDGWYFLAGGKVQAPGIGAMSAGLLIGDYSKMPGDVSGALMQFAYDKHVPPSFQNG
                     ISGFFFTGMKELPVLNIPNYSIDLGVISASFGAQAGLDARLWMDFASAGNEYGIGAMV
                     FAHAYLKGASITCTKFGADARAELGMKGSYATSTGTFSLNGCGSFTISGSIKQCFPTP
                     CFSDGICCEGCGGIGVSQGIKVNLLLDSNGATDLSFGFGNCSGQQVMGGSW"
     gene            2786..4768
                     /locus_tag="PKX59_07555"
     CDS             2786..4768
                     /locus_tag="PKX59_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90759.1"
                     /translation="MKRFLLSIIIFCTIINITVAQEQAGSIPVAVPVAQGVWVYLGNS
                     IPRNMHYQIERSKGDSKRFEKLGETTAPSSEQEMKNRQLPFSKYFEKLDPINDKTINR
                     LWQSTQKYPKADSLFSSNLPMMHLMAGTAYFDATAEKNENYIYRISLIASDGKSIFTK
                     ESNATAQFKKASLPKINFESCKYAEGKLVLTWSVKDPLRMSHFNVYRTIFGKEEYAKI
                     SIERGVYSENNNLMLLAIDTIGNKPAWYEYKIAAVDAYGNEGELQAIASGGSVEDYYA
                     PPITNLKAINTNSNHEVRLSWRLEKKKYLNGINVMRSTLFDSGYQRIATLPVSDTSFT
                     DIVPLSGENYYYYLQILSANDKPVSSAKVFAAYSGDKSVPLPPKEIDATSQPKGINVY
                     WKSDEPFAKGFYVYRRDNTTVPFVLISPLIASRQPIYSFSDTSQQLQAGGVYQYIVRT
                     ISDNNQLSKPSDTVSANPGIKKAMTPPTNLRFRENNGMLTLLWDDMRNWESNLMGYKV
                     YRKTSNGAFVKMDNDSLKSERNFFEDSELSSGTSYVYGVKTFDNSGSESEMATISFTA
                     MGELLAGPPSGISVSQTGDNVFITWGQISGDIVAIKIYKSEKGIQPKLIATITDVDSY
                     TDKNVFKGKLYFYQLSTVGKSNKEGAQSEKISIRVK"
     gene            complement(4781..5410)
                     /locus_tag="PKX59_07560"
     CDS             complement(4781..5410)
                     /locus_tag="PKX59_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90760.1"
                     /translation="MLKTGNTHFNIGILNVDSLFADAFLRLLNSFNDNLTIKHVSEAS
                     IASFYSFDILFVHQSRTWDYLNFLLSQKSNINAHETIYCLNCNTSLLLQKDVTDTLKI
                     LDLTIPVNDIRENLQHHVRKINKGKAKFGRPARSLTVLSRNFQLAGHFSDEELNIIYS
                     LRKHICNKQISVSCKISVSTLKRRLKAIADKCGVPNNKAAILCHLYERM"
     gene            5669..7567
                     /locus_tag="PKX59_07565"
     CDS             5669..7567
                     /locus_tag="PKX59_07565"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HOZ90761.1"
                     /translation="MKKIKLIIILLLVSNMLQSQTIVLQETFDGQVMPTGWHEDSAGF
                     APVINQWNFIYYPFTMNGIGFDASFIRTGIANHDYSLVSPSFSTVGLSDVYLSYSDYH
                     QGPIDSGYQNVEMSVDSGQSWTIMLSNNGYNHYLGQNGSRRVVHLGTNAANQPNVKIR
                     FHFKVGGGWRWAIDSVVVNDYQLCTTPTDSTGVVYSSKDFLCSGIAGFFWIDHVSGGI
                     GQTYQWQYKPWGSSSYLNIAGAVYDTMTFVQNTPNYYRCLVVCNADTAGSMVTFVTDS
                     PTVVPKLRATNFSGICPGSPDTTYIFCEKNIPGIGYQWQWKQNIADPFTDIAGANDTL
                     YAIDAAVFPNQIYLRCKQQCIQSGKYLATATEVIFENPNPSCYCIPYNQNYCGPSTPI
                     FDGGIVDMHIAGTTLNNPSSTCSNIYGYPGQLSNYTYFDPTVANQTATLSRNVPYSMT
                     ITTDTLQIWFKGGLWIDYDHDSYFENTEYTTIDSLYPGTPRTITFSIPATATPGLTGM
                     RLRVAGNFPSVLNANSACTYAYGGETEDYMITIDTIVGIGEVKQHEANVQLYPVPASG
                     YIVVQSSQTIRSVRLESTTGALVKQDNGLNTNNWRMDVSSYAPGMYLMRTETSAGVVM
                     KKVMISRE"
     gene            complement(7585..8571)
                     /locus_tag="PKX59_07570"
     CDS             complement(7585..8571)
                     /locus_tag="PKX59_07570"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HOZ90762.1"
                     /translation="MAMLLELTNKGIYCPQADVYIDPHRRVENAIITHAHSDHARPGS
                     SHYLAHKDSEAMLRMRLGEQISLRTVAYGESFTINGVKFSLHPAGHIIGSAQIRVAYK
                     DEVWVAAGDYKLHDDGFCPVFEHLKCNVFITESTFGLPIYKWQPQQQVMQEIDTWINK
                     NNQQQITSVLMGYSLGKMQRLIKHLNHFDFGIYGTPDVVAVNDRLREAGCNLPYVKPL
                     KSNPEKENLKGALIFASPSGARSALFKKLDNYSVGYCSGWMLHGKPKYLSHVDRGFAL
                     SDHADWDELNTAVKESGAEKIFVTHGSTASFSKWLCDNGYDAKEIELMKGAG"
     gene            8673..10049
                     /locus_tag="PKX59_07575"
     CDS             8673..10049
                     /locus_tag="PKX59_07575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOZ90763.1"
                     /translation="MNQTMRLILLGLLVFASRWFFLDAGYGAEEDAWGYAVNALAMDS
                     TNSYQYSRLPGHPVPEWVYYFLPVKHSFAFNLCTAMLSTIAVLLFYLICKKYKLNAIA
                     GALMLAFTPVFFIQSTNAMDYNWSLCFMLAALFFAGKRNMILTSIMLGLAIGCRITAV
                     LFIIPISIYLLKADRSWKLVLQLLFLSGVVSALLYLPLILKYNLAFLTYVNQFGYPQI
                     LKTIYKASFGVWGTLGFVVIIIAFSYALQNIFNGMHSRKYKPILLCAASTLIIYTLLF
                     FYEPHKSAYLIPVIPFVFLIILIALQDKVWPVVITASLVISCFLGGINLADSHRSALP
                     GKYAIVRKAGSQLIAFDPLSGNVTDDHSKRIQRIKYAGSVVEQAMQLKGKNALICGYW
                     INMVSILGIGQLSDKQTLLHYADEQELIKLKNANTEIYYIPGQDSFNDVCFKNVFTRK
                     WAKSLFPQ"
     gene            10210..11037
                     /locus_tag="PKX59_07580"
     CDS             10210..11037
                     /locus_tag="PKX59_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOZ90764.1"
                     /translation="MIKKPKVLFVSQEIFPYLESSTIGNIARHLPQGTQEKGKEIRTF
                     MPRFGVVNERRNQLHEVIRLSGMNLIIDESDHPLIIKVASIQAARMQVYFIDNEEYFQ
                     RKHTTHDADNNFFKDNDDRTIFFCRGVLETVKKLGWAPDIIHCHGWMSSLIPLFVKTA
                     YREDPIFRNARVVYSVYDDEFSESFHDSYSKKLMMEGISTNDVKDIKSPNFESITIAA
                     INKSDAIIKGAEKLNTKIDNHIKKSGKLILPYQSPENYVEAYNTFYDEIMEEISIEA"
     gene            11111..12379
                     /locus_tag="PKX59_07585"
     CDS             11111..12379
                     /locus_tag="PKX59_07585"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HOZ90765.1"
                     /translation="MACVVLLSISCNKPDSVGLNLLPGEDAMNVEFFDTLTLQTSYKQ
                     EDSLRTDAHLLYELQATNHQFMLGKYVDPIFGETSAELFTQLKPTAVFSNTNKTYDSL
                     FLNLAYISNYGDTSALQTFEVYELTDAMYDTASYYAFSNLNYSTKVGEITFKVEDAQD
                     SIPTSDTTKIAPRLRLRLDDVLGQKIFNSDLSDPSKFKETVKGFCIKPAASSFGSIIN
                     FDAKTTVSSMIMYFTDPATTGIDSLPFYIYSSDGSIARFSRFTHDYSAFNFTNPLTDK
                     AYVQTMGGIKTKISIPYLNSLKEMGPISINKAELIINVDPGTATNLPAPDALSLYAID
                     SAGQIQTLPDLTRSSTLFGGGLANNQYRFNIAQFLQQILYEKRIDYGMYLRVRVPVAF
                     QTPNRVALVGGDPASTLRMKLQITYTKLNP"
     gene            12386..14221
                     /gene="glmS"
                     /locus_tag="PKX59_07590"
     CDS             12386..14221
                     /gene="glmS"
                     /locus_tag="PKX59_07590"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOZ90766.1"
                     /translation="MCGIVAYIGYRQAYPIVIKGLQRLEYRGYDSAGVALLNGELSVY
                     KKAGKVADLANYVSDKNLVGQVGMGHTRWATHGEPNDRNAHPHYSESKDLAIIHNGII
                     ENYAAIKQELINSGHTFKSDTDTEVLIHLIEEIKHKTHVATEEAVRLALGEVIGAYAI
                     VVLSKSEPNKMIAARKGSPIVVGIGKDEFFIASDATPIVEYTKNVAYLNDGEVATIDN
                     GKLTVKTIDNETKIPFVQELELKLEAIEKGGYDHFMMKEIYEQPRSIWDSMRGRIDAD
                     KGRLRLGGISEFENKLINAKRIIIVACGTSWHAALVAEYLIEDLARIPVEVEYASEFR
                     YRNPIISEDDVVIAISQSGETADTLAAIEMAKERGATIFGVCNVVGSSIPRATHAGSY
                     THAGPEIGVASTKAFTAQVTVLTLMALQIANKRGTITQSRFRALLNELETIPSKVEIA
                     LKSNDKVKEISALFKDSRNFLYLGRGYGFPVALEGALKLKEISYIHAEGYPAAEMKHG
                     PIALIDEEMPVVVIATKNSSYEKVVSNIQEVKARKGKIIAIVTEGDVDVRKMADYTIE
                     IPETDDILVPLVSVIPLQLLSYHIAVMRGCNVDQPRNLAKSVTVE"
BASE COUNT         4621 a         2578 c         2770 g         4282 t
ORIGIN      
        1 caaatcacct ctacaacaga tgatatttct tgtttcggta tttcaacagg cagtgatgga
       61 aaatatgtac ttaaaggtgt ttctacttca ccgaaagaaa aaacagtttg ggcttgtaaa
      121 cccggtgcta cgcccaacat catcagccag aataaaaaaa ttacattaag tcagcagaat
      181 gaattggatt ttaatttgat tagtgataat gaactcatta ttgaaaatat ttttggattt
      241 gaagtggaca ttcaatccaa agtgaaacaa tcggacggca tttggttggt gagtggtaat
      301 ttgataaatc tgcctgctaa tgataatttt tcattgcacg acagcaaaca gaaaatatct
      361 ttcaccgatt tgaaaataaa aaagagtgat gctacaaaga atggaattcc tgttggtgtt
      421 tctgtggaaa acaccttttc aaccgatctc acaaatatta aattgctttt gcaaaattcc
      481 tttggagtaa ttcaaaagcc ctcttcaggt gatgtgttac aggtaaaatc cgataatcag
      541 aaaggtagat taacaggaaa aatagctgta caaaaatcgt catttaaatt tacacagagt
      601 tatatcactt tcaataatga tcctaatgaa gctatgttga ttactgataa accgggttcg
      661 tttataactg atatttcttc gattgatatt gcaacttcca ctaaaaagaa attcggattg
      721 gctaatctgc aaggaaaaac aatgaagttt tccttacttg gttttgatgc agaggcagat
      781 gctattaatt cctggctaca ggacaacagt attaatttac aaaccatcat tcatatcaat
      841 gcactgccgg acatgactcc ttctacactc gatataaatg caggaaacct cataattcat
      901 cctgagaagt tggagccact taaaggcaat caacctataa aattcaattt agaaaagtgg
      961 caattcatag gtaataactg gcaactgtca caaggcgata attcaattaa cattgctacg
     1021 ggcacaatta aaaccggaag tatagatatt cccatatctg atatttcttt acagccggat
     1081 catattggca ttggagattt tgatgtaaac aatctttcat tcggtggtat cattcctgtt
     1141 catgtaacat caaccaatcc tgtattcgga cgaaacaaaa gtattggctc agatcagaaa
     1201 ccacattatg aatttcgttt gataggtgag aatggaagcc ccggtgtagt cattaaaaca
     1261 ttgcccggaa tgaagaccgg tgaagaaatg aaatttcaaa atctttcatt aatcagcaat
     1321 ggagaacaaa ttataaatcc gggtaatcaa ccaaactcaa ttacatttta tagtgtgatg
     1381 aaagtaaaac cactttcatt tatgagtgga caggattatg taaatatgac ttgtggtata
     1441 gaccttggaa ttccacagtt aaaagaaacc agcggcatca ttcaattttc gaaagaagca
     1501 ggacaaatta aatttcagtt gtatccatta aatgtatcac taaaaggacc gggagatgtt
     1561 gacttcgttg caaatgttca gtttaatgat cgtccgcaaa atcttactga aggaaaattc
     1621 actgcacttg gtactattta tgaaaaagaa ggaatggttt taaaagcaat tttaaacaaa
     1681 actactacag cagcatggat acaagttgat cctgaaaatc aaaaattacc attaggtgga
     1741 agcactacta gtcttgccaa cattaaagga aaaatggaag ccgatatgag ttccggaata
     1801 tggaaaaatt ttactttcag tggcgaaatg caaggcttca aaggaatgca aggtgataca
     1861 agaaaaacat ttgtagtgac gggttccatc aatgcaagta atcaaaatat tgaagtaaaa
     1921 aatattcctt ccggatttgg aaatattgga cttaactatg attttgtaaa cagccgattc
     1981 acaggtaatt tacagttaga taaaaatata ggaccattag ctttagcagg tactgctaac
     2041 ttattggttg atcctgatgg atggtatttt cttgcaggag gaaaagttca agcacccgga
     2101 attggtgcca tgtctgccgg attgcttatt ggagattata gtaaaatgcc cggtgatgtt
     2161 tccggtgcac tcatgcaatt tgcatacgat aaacatgtgc caccaagttt tcaaaacggc
     2221 atttcaggat ttttctttac aggtatgaaa gaattgccgg tgctaaacat tccaaactat
     2281 agtatagacc ttggtgttat cagtgcttcg tttggcgcac aagccggatt agacgcacga
     2341 ttatggatgg actttgcttc ggcaggaaat gaatatggaa ttggtgcaat ggtgtttgca
     2401 catgcatatt taaaaggtgc ttcaattaca tgtaccaagt ttggcgcaga tgccagagca
     2461 gaattaggaa tgaaaggaag ttatgcaaca tctaccggta cattttcttt gaatggttgt
     2521 gggagtttta ccataagcgg aagtataaaa caatgctttc ctactccttg ctttagtgat
     2581 ggaatttgtt gcgaaggttg tggtggaatc ggtgtaagtc agggtataaa agttaattta
     2641 ttgttagact ccaatggtgc tactgacttg agctttgggt ttgggaattg cagtggacaa
     2701 caggtgatgg gtggcagctg gtaaatccaa tcttgtactt tagaatagat tatttaaatg
     2761 gtgaataaaa caaaatcttt aaaaaatgaa acggtttctt ctttcaatta tcatattttg
     2821 tactattatt aatattactg ttgcacaaga gcaagccggg tcaatacctg ttgctgttcc
     2881 tgtagcacaa ggggtgtggg tttatttagg aaatagtatc ccacgaaaca tgcactatca
     2941 gattgagcgt agcaaaggcg atagtaaaag atttgaaaaa cttggtgaaa caacagcacc
     3001 ttcatcagaa caggaaatga aaaaccgtca actccccttt agtaaatact ttgaaaaatt
     3061 agatcctata aatgataaaa caatcaatcg cctttggcaa tcaacacaaa aatatccaaa
     3121 agcagacagc ttgtttagca gcaatcttcc aatgatgcat ttaatggcag gaactgcata
     3181 ctttgatgca acagcagaaa aaaatgagaa ttatatttat cgtattagtt taattgcatc
     3241 tgatggcaaa tctattttta caaaagaaag taatgcaact gctcaattta aaaaagcatc
     3301 tctgccaaaa ataaatttcg aaagctgcaa atatgcagaa ggaaaacttg ttttaacatg
     3361 gagtgttaaa gaccctttga gaatgtcaca cttcaatgtt tatagaacaa tatttggtaa
     3421 agaagaatat gcaaagattt caatagagcg aggtgtttat tctgaaaaca acaacctgat
     3481 gttacttgct attgacacta ttggaaataa gcctgcatgg tatgaatata aaattgctgc
     3541 tgttgatgct tatggtaatg aaggcgaact gcaagccatt gcaagtggag gtagtgtaga
     3601 agattattat gcaccaccaa taacaaatct aaaagcaatt aataccaaca gtaatcatga
     3661 ggtaagactt tcctggcgac ttgaaaaaaa gaaatatctg aatggaataa atgtaatgcg
     3721 cagcacactg ttcgattcag gatatcaaag aattgcaaca ttgccggtga gtgatacttc
     3781 ttttacagat atagttccat tatcaggtga aaactactat tactatctac agattctaag
     3841 tgctaatgac aaacctgttt cttctgcaaa agtgtttgca gcttattccg gagataaatc
     3901 agtacctctg ccgcccaaag aaattgatgc tacatcacaa cccaaaggga taaatgttta
     3961 ttggaaaagt gatgaaccat ttgccaaagg attttatgtg tacagaagag ataacacaac
     4021 cgttccgttt gtattaataa gtccattaat tgcttcaagg caacctattt actctttttc
     4081 agatacatca cagcaacttc aagcaggtgg tgtttatcaa tatattgttc gaaccatcag
     4141 tgataacaat caactgagca aaccttcaga tacagtaagt gctaatcccg gaataaaaaa
     4201 agcgatgact ccaccaacta atctgcgctt tagagaaaat aatggcatgt taacattact
     4261 gtgggacgac atgcgcaatt gggaaagtaa tttaatgggc tataaagtgt atcgaaaaac
     4321 cagtaatggt gcttttgtaa aaatggataa tgacagtttg aaatccgaac gtaacttttt
     4381 tgaagattca gaattatctt caggaacatc ttatgtatat ggtgttaaaa catttgacaa
     4441 ttcaggaagt gaaagtgaga tggcaacaat tagttttact gctatgggtg aattgttagc
     4501 aggtccacct tcaggaattt ctgtaagcca gaccggtgac aatgtgttta tcacatgggg
     4561 acagatttcc ggagatattg tagcaattaa aatttataaa tctgaaaagg gcatacagcc
     4621 caaattaatt gcaactatta ccgatgtaga ttcttataca gataaaaatg tgtttaaagg
     4681 gaaactgtat ttttatcaat tatccaccgt tggcaaatca aataaagaag gtgcacagag
     4741 tgaaaaaatt tctatcaggg ttaaataatc aactcttcaa tcacattctt tcgtagagat
     4801 ggcaaagaat tgctgcttta ttatttggca caccacattt gtctgctata gctttaagcc
     4861 ttctctttaa agtagaaaca gatattttac aactcactga aatttgtttg ttacagatat
     4921 gctttcttaa actgtaaata atattcaatt cttcatcaga aaaatgccct gctaattgaa
     4981 aattgcggct cagtacagta agcgatctgg cgggtctgcc gaattttgct ttacccttat
     5041 ttattttcct gacgtgatgt tgaagatttt ctctaatatc attaacagga atggtgaggt
     5101 caagaatttt taaggtgtct gtcacatcct tttgcaataa cagacttgta ttacagttca
     5161 gacaatatat tgtttcatga gcattaatat tagatttctg actgagcaaa aaattcagat
     5221 aatcccatgt tctggattga tgtacaaata aaatatcgaa agaataaaac gatgcaatgc
     5281 ttgcttcgct gacgtgttta atggttaaat tgtcattgaa agaattaagc aatcttaaaa
     5341 aagcatctgc aaataaacta tcaacattga ggatgccgat attgaaatgc gtatttccgg
     5401 ttttaagcat aataatggtt gtttaagtaa agcaagtata tgattttttt tgataactga
     5461 caagaaaaaa agcggtaagc atataaattg agccaaaagt tcggggttat gaacattgga
     5521 gctattcagg ctttttataa ttttgaaaaa caataaagtt ggttagcgtt catggttgat
     5581 ttatttttat ttaaaaatgt aatgtgactt taatattgaa gcaataaatc ttttaaaaaa
     5641 atcttcatca cacaataaaa acaagaaaat gaaaaagatt aaactcataa taatattgct
     5701 gttagtaagc aacatgttgc aatcgcaaac catagtattg caagaaactt ttgatggcca
     5761 agtaatgcca acgggctggc atgaagacag tgcaggcttt gctcctgtaa ttaatcaatg
     5821 gaattttatt tattacccgt ttacaatgaa tggtattggt ttcgatgctt catttatcag
     5881 aacaggtatt gctaatcatg attacagctt agtatctcct tcgttttcaa cagtaggttt
     5941 atctgatgtc tatctttctt atagtgatta ccatcaaggt ccaattgata gtggttatca
     6001 gaatgtggaa atgtctgttg acagtggcca aagttggaca attatgttat ctaacaatgg
     6061 atataatcat tatttaggac aaaatggttc aagaagagta gttcatttag gaaccaatgc
     6121 agccaatcag cccaatgtaa aaattcgttt tcattttaag gtgggtggtg gatggcgttg
     6181 ggcaatagac agtgtagtag taaacgacta tcagttatgc actacaccaa cagacagcac
     6241 aggagtggta tatagcagca aagatttttt atgctcgggc atagcaggtt ttttttggat
     6301 agaccatgtt agtggcggca taggtcaaac ctatcaatgg cagtacaaac cctggggcag
     6361 cagcagctat ttaaacattg ccggtgccgt ttacgacacc atgacgtttg ttcaaaatac
     6421 gccaaactat taccgctgtc tggtagtctg caatgccgat acagcaggct ctatggtaac
     6481 ctttgttaca gactcgccca cagtggtacc taagttaagg gcaacaaatt ttagtggtat
     6541 ttgtcccggc agccccgata caacttatat cttttgcgaa aaaaatattc caggcatagg
     6601 ctatcaatgg caatggaaac aaaacattgc agaccccttt acagatattg caggagctaa
     6661 cgatactttg tatgccatag atgcagcagt gtttcctaat cagatatatt tacgctgcaa
     6721 acagcaatgt attcaaagcg gcaaatacct tgctacagca acagaggtaa tatttgagaa
     6781 tcccaatcca agctgctact gtattcccta caatcaaaac tactgcgggc cttctacccc
     6841 aatttttgat ggcggtattg ttgatatgca catagcagga acaacgttaa acaatccgtc
     6901 ttcaacatgc tcaaacattt atggatatcc cggtcagttg agcaactaca cctactttga
     6961 cccaacggtt gcaaatcaaa cagcaacact gtcgcgcaat gtaccctata gcatgactat
     7021 tactacagat accttacaaa tatggtttaa aggcggactg tggatagact acgaccacga
     7081 cagttatttt gagaacacag aatatactac catagactca ttatatcccg gcacaccaag
     7141 gacgattacc tttagtattc cggccacagc cacacccggc ctaacaggaa tgcgcctgcg
     7201 tgtagcaggg aatttcccat cagtactgaa cgctaacagc gcctgcactt atgcctatgg
     7261 aggcgaaaca gaagattata tgataaccat agacaccata gtgggcattg gcgaagtaaa
     7321 gcagcatgag gctaatgtac agctatatcc tgttcctgca agcggttata tagttgtgca
     7381 atcatcacaa acaataagaa gtgtgaggtt agagagcact acgggagcat tagtaaaaca
     7441 ggataacgga ttaaacacaa acaactggcg aatggatgtt agcagctatg caccgggtat
     7501 gtacctgatg cgcacagaaa cttctgccgg tgtagtgatg aaaaaagtaa tgataagcag
     7561 ggagtaaaaa cagttttatt cttcttaccc cgcccccttc atcagttcaa tttcttttgc
     7621 atcatatcca ttgtcgcaaa gccattttga aaatgatgcc gtacttccat gtgttacaaa
     7681 aattttctcc gcaccacttt ctttaactgc tgtattcaac tcatcccagt cggcatggtc
     7741 gcttagtgca aaaccacggt caacatggct caagtatttc ggtttgccat gcaacatcca
     7801 tccactgcaa tagccaacac tatagttgtc gagtttctta aacaatgccg aacgtgctcc
     7861 ggatggtgat gcaaatatta atgccccttt cagattttct ttctcagggt ttgatttcag
     7921 cggtttaaca taaggcaaat tgcaacctgc ttcgcgcaat ctgtcgttaa cagcaacaac
     7981 atccggtgtt ccataaatgc caaaatcaaa atggtttaag tgctttatca gcctttgcat
     8041 tttgccaagc gaataaccca tcagtacaga agttatttgt tgctgattat tcttatttat
     8101 ccaagtatca atttcctgca ttacctgttg ttgtggctgc catttgtaaa taggtaaacc
     8161 aaaggttgac tctgtaataa acacattaca ttttaggtgt tcaaacacag ggcagaaacc
     8221 atcatcgtgc agtttgtaat cgcctgctgc cacccatact tcatctttat atgctactct
     8281 tatctgtgca ctgccaataa tatgaccggc aggatgcaaa gaaaatttta ctccgttaat
     8341 tgtaaatgac tctccataag caaccgtgcg taaactaatt tgttcgccca atcgcatacg
     8401 cagcatggct tccgaatctt tatgagcaag atagtgactg cttccagggc gagcatggtc
     8461 gctatgtgca tgtgtgatga tggcattttc tactctgcga tgtgggtcaa tatagacatc
     8521 tgcctgcgga cagtaaatac ccttgtttgt caactctaat aacatagcca tgacggtgct
     8581 gcaattattt gtaattacgt aaagatataa atgtgttgtt aaaattgaat caacatcata
     8641 taaaaatggt caaaacatgt tactttgcgt ccatgaatca aactatgcgg cttatccttt
     8701 tgggattatt ggtttttgcc agccgctggt tttttctcga tgcaggttat ggtgccgaag
     8761 aagatgcatg gggctatgcc gtaaatgctc ttgcaatgga ttcaacaaat agttatcagt
     8821 attcgcgttt gcccggacac cctgtacccg aatgggtgta ttacttcctg cctgtaaaac
     8881 attcttttgc ttttaattta tgtacagcca tgctgagtac gatagctgtt ttgctgtttt
     8941 atttaatctg caaaaaatat aaactcaatg ctatagccgg agcattaatg cttgctttta
     9001 ctcctgtttt ttttattcaa agtaccaatg ccatggacta taactggtcg ctgtgcttta
     9061 tgctggcagc cttgtttttt gccggaaaac gaaatatgat tttaacctcc attatgcttg
     9121 gtctggcaat aggttgccgc attacagcag tgctgtttat tatccccata agtatttatc
     9181 ttctaaaagc cgatcgttca tggaaacttg tgctgcagct tctgttttta tcaggagtag
     9241 tgagtgcact tttatatctt ccattgatat taaaatacaa cctggctttt ctgacttatg
     9301 taaatcaatt tggctatccg caaattttaa aaactattta taaagcaagt tttggtgttt
     9361 ggggaacatt gggttttgta gttattataa tagcttttag ttatgcgctt cagaatattt
     9421 ttaatggaat gcatagccgg aaatataaac ccatactgtt gtgcgctgct tcaacactta
     9481 taatttatac cttacttttt ttctatgaac cacataaatc tgcttatctt attcctgtaa
     9541 ttccatttgt gtttttaata attctgattg cattgcagga taaagtttgg cctgttgtaa
     9601 ttacagcatc attggtaata agttgttttt tgggaggtat taatcttgca gacagtcaca
     9661 ggtcggcact gccgggtaaa tatgccatag tcagaaaagc aggttcccaa ctcattgctt
     9721 tcgacccatt aagtggtaat gttactgacg atcattcaaa aagaattcag cggataaaat
     9781 atgcaggaag tgttgtagaa caggcaatgc agttaaaagg taaaaatgct ttgatttgtg
     9841 gatactggat aaatatggtt tctattctgg gtataggtca actttctgac aaacagactt
     9901 tgctgcatta tgccgatgaa caggaattga taaaattgaa aaatgccaat acggagattt
     9961 attacatacc cgggcaagac agttttaatg atgtctgttt taagaatgtc tttacacgta
    10021 aatgggcaaa aagcctattt ccgcaataaa tcaaccccaa ttgcccaatt tctacttttt
    10081 tttagtatta aagattaagc cttttgcctg catatcagct actttaacaa aatatcgcta
    10141 aaattttgcc ggttcaaagg gaatttttat atatttttgc atacgtaaat tttaagagaa
    10201 gtagattgca tgattaaaaa gcccaaggtc ctgttcgtat cgcaggaaat tttcccttat
    10261 ctcgaaagtt caacaatagg taacattgca agacatctgc cacaaggcac acaggagaaa
    10321 ggtaaagaaa tcagaacctt tatgccacgc tttggcgttg ttaatgagcg tagaaatcaa
    10381 ttacatgagg taatccgact ttccggtatg aacctgatta ttgatgaaag cgatcatccg
    10441 ttgattataa aggttgccag tattcaggca gcccgcatgc aggtttattt tattgataat
    10501 gaggagtatt ttcaaagaaa acatactact catgatgcag acaacaactt ttttaaggac
    10561 aatgacgacc gtaccatttt cttctgtcgt ggtgttcttg aaacggtaaa aaaactcggt
    10621 tgggcaccgg atattattca ttgccacggc tggatgtcga gtctgattcc gctgtttgta
    10681 aaaacagcct atagagaaga ccctattttc cgaaatgcaa gagtggtgta ttctgtatat
    10741 gacgatgaat tcagtgaatc atttcatgac agttactcta aaaaactcat gatggaaggg
    10801 atttctacta atgatgtaaa agacataaaa agtccaaact tcgaaagcat aacaattgct
    10861 gccataaaca agtcagatgc tatcattaaa ggtgctgaaa agctcaatac caagattgat
    10921 aatcatatta aaaaatcagg caaattgatt ttaccatacc aatcgcctga aaactacgtt
    10981 gaggcataca atactttcta tgatgaaatc atggaagaaa tctcaataga agcctaagcg
    11041 gatattgttc aacattaatt attttaaaaa accacgcctt atttatatgc aacactttaa
    11101 gcgtttgtgg atggcatgtg tcgtgcttct aagtatctca tgcaacaagc ccgactctgt
    11161 tggtcttaac ctcttgcccg gagaagatgc catgaatgtt gaatttttcg acacattgac
    11221 acttcagaca tcgtacaagc aggaagacag cctgcgcacc gatgcacatc tgctttatga
    11281 acttcaggct acaaaccatc agtttatgct cggcaaatat gttgacccta tttttggtga
    11341 aactagtgct gaacttttta cgcaacttaa gccaacagca gttttttcca ataccaacaa
    11401 aacatacgat tcattatttc tgaatctggc atatatttca aactatggcg acacctccgc
    11461 cttgcagact tttgaagttt atgaactgac tgatgctatg tatgacacgg caagttatta
    11521 tgctttcagt aatctcaatt acagtacaaa agttggcgaa ataacattta aggttgaaga
    11581 tgctcaagac agcattccta cttctgacac aacaaaaatt gcccctcgac tgcgtttgcg
    11641 ccttgatgac gttttaggtc agaaaatatt taattccgat ttaagcgacc catcaaaatt
    11701 taaagaaacg gttaaaggtt tttgtattaa acctgcagcg tcatcattcg gaagtattat
    11761 caattttgat gcaaaaacaa ccgtttcgtc aatgataatg tattttacag atccggcaac
    11821 aacaggcatt gactcactac cgttttatat ttacagctca gatggcagca tagcccgttt
    11881 cagccggttt acacacgatt actcagcatt caattttacc aatccattga cagataaagc
    11941 ttatgttcag acaatgggtg gtattaaaac aaaaatttcc attccttatc ttaacagtct
    12001 gaaagaaatg gggccaatat caattaataa agcagaatta atcatcaatg ttgatccggg
    12061 aacggcaacc aaccttcctg ctcctgatgc attgagtctt tatgccatag attccgcagg
    12121 acaaatacaa actttgcctg atttgacacg ttcatctaca ctttttggcg gtggactggc
    12181 aaataatcag taccgtttta atattgcaca gtttttgcaa cagattttat acgaaaaacg
    12241 tatagattac ggaatgtact taagagtaag ggtgccggta gcctttcaaa cacctaatcg
    12301 tgtggcttta gtgggaggcg accctgcttc aacattacga atgaaattac aaattactta
    12361 tacaaaatta aatccataaa atatcatgtg tggaatcgtt gcctatatag gatatcgtca
    12421 ggcttatcca atagtaatta aaggacttca gcggctggaa tatcgcggct atgacagtgc
    12481 cggtgtggca ttgttgaatg gtgaattgag tgtttacaaa aaagcaggta aggttgccga
    12541 cttggccaac tatgtttctg ataagaacct ggttgggcag gtaggtatgg gacatacacg
    12601 ttgggcaaca catggcgaac ccaacgacag aaatgcgcat ccgcattatt ccgaatcgaa
    12661 agatttagcc atcatccaca atggtattat cgaaaactat gcagcaataa aacaagagct
    12721 gataaattcg ggtcatactt ttaaaagtga tacagacact gaagtgctga tacatcttat
    12781 tgaagaaata aaacataaaa cacacgtagc tactgaggag gctgtacgtc ttgctttagg
    12841 cgaagttatt ggagcttatg ccattgtagt tttatcaaag agtgagccta ataaaatgat
    12901 tgcagcccgc aagggtagcc ctattgttgt gggtattggt aaagacgaat tttttattgc
    12961 atctgatgca acacctattg tagaatatac taaaaatgtt gcctacctca acgatggcga
    13021 agttgcaaca attgataatg gtaaacttac cgtaaaaaca attgacaacg aaaccaaaat
    13081 tccttttgtt caggaattgg agttaaagct tgaagctatt gaaaaaggtg gctacgatca
    13141 tttcatgatg aaagaaatct atgagcaacc tcgttctatt tgggacagca tgcgcggacg
    13201 cattgatgct gacaaaggca ggttgagatt aggtggtatc agcgagtttg aaaacaaatt
    13261 gatcaatgca aaaagaataa tcattgttgc ctgcggaaca tcttggcatg cagcattggt
    13321 agcagaatat cttattgaag acctggcacg aattcctgtg gaggttgaat atgcttctga
    13381 attccgctat agaaatccaa tcatctctga agatgatgta gtgatagcta tttcgcaaag
    13441 tggcgaaact gccgatacac ttgctgccat tgaaatggct aaagaacgtg gtgcaacaat
    13501 ttttggggtt tgtaatgttg tggggtcttc tattccacgc gcaacacatg ccggttccta
    13561 cacacatgcc ggacccgaaa ttggtgtggc ttctaccaaa gcatttactg cacaggtaac
    13621 agtactaaca ttaatggcac ttcagattgc aaacaaaaga ggtaccatca cacaatcacg
    13681 ttttcgtgca ttgcttaacg aacttgaaac cattccttca aaagttgaaa ttgcacttaa
    13741 atcaaacgat aaggttaaag aaatatctgc attgtttaaa gactccagaa acttccttta
    13801 cctaggtcgt ggttatggat ttcctgttgc attagaaggt gcattaaagc ttaaagagat
    13861 atcctacata catgcagaag gttatcctgc tgcagaaatg aaacacgggc ctattgcttt
    13921 gattgacgaa gaaatgcctg ttgtagttat tgccacaaaa aattcttcat acgaaaaagt
    13981 ggtttcaaac attcaggaag taaaggcacg taaaggaaaa ataattgcaa ttgttacaga
    14041 aggcgatgtt gatgtaagga agatggcaga ttatactatt gaaattccgg aaacagacga
    14101 cattcttgta ccattggtta gtgtaattcc attgcaattg ctcagttatc acattgctgt
    14161 tatgcgtggt tgtaatgttg atcagcctcg taacctggca aagagtgtta ctgttgaata
    14221 acgttgcatt taaactactt ctctcatcat a
//
LOCUS       DAOOOI010000054        14149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_855_length_14149_cov_15.736413, whole genome shotgun sequence.
ACCESSION   DAOOOI010000054 DAOOOI010000000
VERSION     DAOOOI010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14149
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_855_length_14149_cov_15.736413"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKX59_07595"
     CDS             <1..242
                     /locus_tag="PKX59_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90767.1"
                     /translation="LKYANAKNITILFLIKDNQFNMEIADDGIGFNMDLLEESGIKGN
                     GLVNMRNRMMQHNCSLQIVTSENEGCKIIAQSKLY"
     gene            293..>725
                     /locus_tag="PKX59_07600"
     CDS             293..>725
                     /locus_tag="PKX59_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90768.1"
                     /translation="MISFVFLFGISNAQSNSYDSLSKAVNTFQPAKKFELADTIYLKN
                     IEQLNKLFFEVGESDSMRNYALKGLMFVKKQKAAATEETMLESLSNFEMVFNRQAGIS
                     YFDKGDYTNQLKYFQQFLVCAQNINSIKDIATAYVYIATCHR"
     assembly_gap    726..735
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <736..2185
                     /locus_tag="PKX59_07605"
     CDS             <736..2185
                     /locus_tag="PKX59_07605"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOZ90769.1"
                     /translation="LEDLEKSFDYAKQAVQILKGDSFPSILGSAYALSSNYYIEEKIN
                     NDSALYYRKQAYLLFKKANNPMLLTSATFDMIDFFHSIRQTDSCKKYLAEIEPVIQEL
                     ENPEQTMVFNTLSAQVKLSEGKVANAIKMLRKARAIADKTDELNDNSQINRVLALALA
                     ADRKIAVAYNVMDSAFDEYSEDLNTEKVRSLTQAQMNFEFEKERTIAAIEIKRQKQQH
                     YFLIAIASVLLVFAGFVFQRYRERHRSSLILAEKNKTIEKAYRELKETQHNLVETEKQ
                     REAQSIRVRIARDIHDEIGSGLTKITLLSDVAKKKSNQEEVAEALSKITTYSKGVSSS
                     LSEIVWAINPGHDNIASLINYMKSTANNLLEDSGVNYSLHFPDTEISSSIHPEIKRNI
                     FLVMKEAINNSLKYANAKNITVTFTLNKSNFKLIVADDGNGFDVTDFNKIIRNGNGLI
                     NMQQRMFQHQNKLTIISTPGHGCKIVAEGKQI"
     gene            2213..2854
                     /locus_tag="PKX59_07610"
     CDS             2213..2854
                     /locus_tag="PKX59_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ90770.1"
                     /translation="MKKIKIILVEDDKEIRELAEAILGSEKDMEVIKTFSNGENFLKS
                     WENFDCNVVVMDINMPGKTGIECVAEAKPKKPSVQFLISTVFENPQYIFQALCNGATG
                     YVLKNNTGDKLVSAVREIASGGSPMSASIARLVVDSFSHKQTTVINNEILTSREKEVL
                     DLLAQGLMYKEIAAKKEIAVETVRKHVYNIYEKLQVDSRTAAINKVYGKHPES"
     gene            2983..5037
                     /locus_tag="PKX59_07615"
     CDS             2983..5037
                     /locus_tag="PKX59_07615"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOZ90771.1"
                     /translation="MTNKYSLFFSFVLFLIHIRIQAQNVGINNPTPDASALLDMVSTD
                     KGILIPRMTTTLRITIAAPATGLLVYDSDLNKFYFFNGIAWIAVGSNIGWSLTGDAGT
                     ASGTNFLGTTDNQPLLIKVNNTKAGFIDQQEGTFLGRNVFLGKLAGFNNTITGFDNTF
                     IGAKAGEANTIGTLNTAVGAKSLQNAVATFSNTAIGRETMQQCISCVHSTAVGAGALQ
                     NIEGGFWNNAFGYLAMRQAANPGISNNAFGAETLFNNTGSDNCAFGHTALRQNTSGTY
                     NIAFGSLSLYENNTGSYNIGMGAFALQNNSTGSYNTVLGYKCAADGLSNNRVVAIGDS
                     TLYFNKASGIVAVGSRALRSNNGGTSNTAVGYESLYTNTIGNSNTALGDFSLKLNIVG
                     NANTAIGSLALQNNNGNNNTAVGSGAQSANTLGKDNSSVGALTLTNNLSGNFNIAIGS
                     NSLAQNISSHNNVGVGYNTLSAYNASGNTGNNTAIGTGAGDSYTSYSNCTFVGYQADA
                     NAASFSNSTAIGNGAIATASQQVKIGNATVTAIGGAVNWSIISDGRFKTNVKNNIPGL
                     NFINRLNPVSYNYDIKSYCSFIGTDEHSFNDDAAIQQKEKIIYSGFIAQEVEEAAKNS
                     GYNFSGIHKPENTNDTYTLSYADFVVPLVKAVQELTIQNVNLLKKIELLEAKYDLHKK
                     QD"
     gene            complement(5282..6397)
                     /locus_tag="PKX59_07620"
     CDS             complement(5282..6397)
                     /locus_tag="PKX59_07620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ90772.1"
                     /translation="MRVLHFIWSANFGGIEKLVITLAQQQQLNENIKVSLLIGIRKGN
                     FLEMIEQAGLHCEFADMKSGLDFSSSKINRLTKLMSSYDVLHIHTFNPAIAYAAIKSK
                     IKIVFTVHGNFGFGRKRTIKDKVLQFLSRYFIRNHVNHITYNSEFCRNIAMEFYKVKK
                     STPATLIYNAIPNIELPIQDENDPAIKIKSSDFIVGTASRFAGNKRIDRLIEAFEIFS
                     KNKQDTTLLLTGDGVKMQELKNIVKIKKLENQVVFTGFKKNVFAWFNKMNVCVFPFEN
                     EAFGIVAIEALMLGKTVIVFSDGGGITEVIKPLNRLNIVESTAHLVDRLNFYYDNRNV
                     LADEQNNNRQYASRFNISSNEKAYFDVYKEVLNNTLE"
     gene            complement(6744..7739)
                     /locus_tag="PKX59_07625"
     CDS             complement(6744..7739)
                     /locus_tag="PKX59_07625"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOZ90773.1"
                     /translation="MEKIRAAITAVHAWVPDYVLTNKELETMVETNDEWIQTRTGIKE
                     RRILKDAGEGTSKIGVESVNGLLKKRGIQAEEIDLLICATTTPDLVFPATANIICDKV
                     GAKNAFGYDIQAACSGFLFALTTASKFVESGQYKKVVVVGADKMSAIIDYSDRATCII
                     FGDGGGAVLLEPDTNGYGIYDAVLKSDGSGRHFLHQKAGGSVKPPTHATIDAKEHFVY
                     QEGQTVFKFAVKGMADVSAEIMERNNLSADDIAWLVPHQANKRIIDATANRMGIGMEK
                     VMLNIQKYGNTTNGTLPLCLWEWEKQLHKGDNLILAAFGGGFTWGAVYLKWAYNS"
     gene            complement(7749..8714)
                     /gene="plsX"
                     /locus_tag="PKX59_07630"
     CDS             complement(7749..8714)
                     /gene="plsX"
                     /locus_tag="PKX59_07630"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOZ90774.1"
                     /translation="MRIGVDIMGGDYAPLETTLGAIAALNDLSPQEVHLVLIGDEKKI
                     TEIIEGQGVNPKSFTIVNATEVIEMSDSATRAFSQKPNSGMAIGFGLLKQNKIDAFAS
                     AGNSGAMLVGSMFSVKAINGIQRPAISTILPQEDGSTGILLDVGANADCKPEVLQQFG
                     LLGSLLAEHVYHIASPRVALMNIGEEEEKGNLLSQQAHQLLKNTKEINFIGNVEGRDL
                     FNNKADVIVCDGFTGNVMLKEAESLYTLLKKRGIKDAYFDRFDYENYGGTPVLGVNGN
                     VIIAHGISKSKAIKNMILLAKEVTEAHLAEKIATFFQNNVVNQTL"
     gene            complement(8733..8918)
                     /gene="rpmF"
                     /locus_tag="PKX59_07635"
     CDS             complement(8733..8918)
                     /gene="rpmF"
                     /locus_tag="PKX59_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOZ90775.1"
                     /translation="MPNPKHKHSKSRRDKRRTHYKAEAPTIAICPTTGETHLYHRAYW
                     HEGKMYYKGKVVLEKAV"
     gene            complement(8938..9438)
                     /locus_tag="PKX59_07640"
     CDS             complement(8938..9438)
                     /locus_tag="PKX59_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOZ90776.1"
                     /translation="MSKYNLHYAGLKEGIHEFDYLLNQDFFDMFEHPIVQGAHVLIHL
                     TVNKSTTIVVLEIKAKGTVHVQCHRCLEEFEMPLDISKAIIVKTSGEDKEVDDDNIIL
                     ADNAHDIHLAQHFYDVVSLAIPIKVTHPDNGCNEETLRTLEKYMVKNENTNDPRWDIL
                     KNVSNN"
     gene            9824..11185
                     /locus_tag="PKX59_07645"
     CDS             9824..11185
                     /locus_tag="PKX59_07645"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HOZ90777.1"
                     /translation="MDLLFYTHESKLYGLTIFVSIVTFQIVYVFVQWLIKHRRDYLFY
                     IAYLFFVIVFGLSHYDKYLNFYPFSGIAERYYEYINLSAPLLCILLYFRFMREFLNLK
                     HRRPDINTMVKNLEFFLIAYTLLSPFFIWLSLDIAVWKMIFNVVAIITITAGLFIIIN
                     FLKKSIPLDRFAMTGASLIAVGSIFDIALSFLEFRGFNIPFDIHLPLMVCVIGELTTF
                     TLGLAFKTQFVENEKLFVEKNFLSEKSEKQQLQLDILKLRNNLARELHDDIGAQLSLA
                     RMLMSKARADGNERVVDQSMQLLEESINKLRSVTNELENTTLQNEGFQKATEKLLSTI
                     SEFQPIHCNFYAKGVEKRLHLDVEYHLFRITQELINNTLKYAHASIISIIVLGDRDKF
                     YFIYEDNGLGFDLKNSGRGKGLNNILQRVKELGTKTEFIAQPGGGMRCEFKIDYEQSM
                     QIA"
     gene            11195..11860
                     /locus_tag="PKX59_07650"
     CDS             11195..11860
                     /locus_tag="PKX59_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ90778.1"
                     /translation="MALSNLKILIADDHKLFAEGLTYLLKQELPIESIDTVYNGKSAI
                     GKVLNTDYDIIIMDVNMPLLNGIETCAAIKRFRTQTKIVFVSMKADMLTVAQALKAGA
                     DAYILKGNDHNDFITAFKQINNNEIFLSEQIKHYFQDDNPNQIRKNAIACVDYSNSLI
                     SVREKEIIKLVCDGLTSDKIAEVLSISTRTVDTHRNNILNKLKLNNIASLVRFALENK
                     LVD"
     gene            12147..12767
                     /locus_tag="PKX59_07655"
     CDS             12147..12767
                     /locus_tag="PKX59_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90779.1"
                     /translation="MKKLIIVLSVAATAAIIGCSKDDLTSPQALQNKNTTTATNDVSA
                     TDERLINSGTVLVPAPNQGYVKVFLFGAAKLSADKPETFVDINKRDVSQRAFDASFKA
                     MYVKFNERWIYVPGIIPNYGIEGKLTYRVSSSQASDPDATNQILAWHRFTLTIRNLNN
                     RPYTGRLVSLSNIKVLIIPAKSEPGGHDPDLRNYEATMTHFGLPLD"
     gene            12949..>14149
                     /locus_tag="PKX59_07660"
     CDS             12949..>14149
                     /locus_tag="PKX59_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HOZ90780.1"
                     /translation="MTENENFQKIKHLYNRAGFGLHYSELQKLSRTKLNKLVADMIEK
                     AKTDVPITTIAPGHVKSVMLKNAGLTKKEGLSPEQLKERQKLLVSLNKQLKNLNTEWF
                     FRMMDIDSPLREKMTLFWNNHFACREEGNPYFAQVLNNIQRKHALGDFRQLLLEVSQS
                     ASMLNFLNNQQNKKGRPNENFARELMELFTLGRGNYSEKDIKESARAFTGWVHDAEGN
                     FVFNTKNHDNGIKTFFGKEGNFNGEDIIDMILQKPEAAVFIARKAYRFFVNDVPDETH
                     VQELGNHFYKSKYDISVLMNKMFTADWFYAPENTGNKIKSPVEFIVGLSRQFNVTYKR
                     QEIILQLQRGLGQTLFYPPNVAGWAGGRNWIDSSSLMLRLTIPSQILNDGSLNFSAKT
                     DPDDENIV"
BASE COUNT         4703 a         2477 c         2535 g         4424 t
ORIGIN      
        1 cattgaaata tgccaatgct aaaaatataa ctatactttt tcttataaaa gacaaccagt
       61 ttaatatgga gatagctgac gatggtatcg gatttaatat ggatttatta gaggaaagtg
      121 gtattaaagg taatggtctc gttaatatgc ggaacaggat gatgcaacac aactgttcat
      181 tacaaattgt tacttctgaa aacgagggtt gcaagataat tgcacaaagc aaattatatt
      241 aaagttagtt aattcaatga ttaaaacaca ctacagtttt gctctatcat ttgtgatttc
      301 ttttgttttt ttatttggaa tctcaaatgc tcaatctaat agttatgata gcctttcaaa
      361 agctgttaac actttccagc ctgcaaagaa atttgaattg gcagatacaa tttatcttaa
      421 aaatattgaa caactaaaca aactgttttt cgaagttgga gaatcagact caatgagaaa
      481 ttatgcatta aaagggttga tgtttgttaa aaaacaaaaa gcagcagcta cagaagaaac
      541 catgttagaa tcactaagta attttgagat ggtatttaat cgtcaggcag ggatatcata
      601 ttttgacaaa ggtgattaca ctaatcaatt aaagtatttt caacaatttt tagtgtgtgc
      661 tcaaaacata aatagtatta aagacattgc tactgcttat gtttacatag caacttgtca
      721 tagagnnnnn nnnnnattag aagacttaga gaaatctttt gattatgcaa agcaagcagt
      781 ccaaatactt aaaggcgaca gttttccttc aatattaggt tcggcttatg ccttatcaag
      841 caattattat attgaggaaa aaataaataa tgacagtgca ttgtattata gaaaacaagc
      901 atatcttctt tttaaaaaag caaataaccc tatgttactt accagtgcta cttttgacat
      961 gattgatttt tttcattcga tcaggcaaac agattcgtgt aaaaagtatt tggcagaaat
     1021 agaacccgta atacaagaat tggaaaatcc tgagcagaca atggttttta ataccttgag
     1081 tgctcaggtt aaattgtcag aaggaaaagt tgcaaacgct ataaaaatgt tacgtaaagc
     1141 acgtgcaatt gcagataaaa cagatgagtt gaatgataac agccaaataa accgtgtcct
     1201 tgccttggca ctagcagcag atagaaaaat agctgtggcg tacaatgtta tggattcagc
     1261 atttgatgaa tatagtgaag atttgaatac agaaaaagta cgtagtctta cacaggcaca
     1321 aatgaatttt gaatttgaaa aggaaagaac tattgctgct attgaaataa aaaggcaaaa
     1381 acaacaacat tattttctca ttgcaattgc ttcagtactt ttggtttttg ccggatttgt
     1441 ttttcagcga taccgggaaa gacatcgaag cagcttaata cttgcagaaa aaaacaaaac
     1501 cattgaaaaa gcataccgcg agctaaaaga aacacaacat aatttggttg aaaccgagaa
     1561 gcagcgcgaa gcacaaagca ttcgggtgcg aattgcccgc gatatacatg atgaaatagg
     1621 tagtggacta acaaaaatta cattgttgag cgatgtcgcc aagaaaaaat caaatcagga
     1681 agaagtagca gaagcgttat caaaaatcac aacctattca aaaggtgtaa gttcgtcatt
     1741 aagtgaaata gtatgggcta ttaatcctgg ccatgataat attgccagcc taataaacta
     1801 tatgaaatct acagcaaaca atctattgga agattccgga gtgaattact cacttcattt
     1861 tcctgatact gaaatatctt cttccattca tcctgaaatt aaaagaaaca tttttttagt
     1921 aatgaaagag gcaattaaca attcattgaa atatgccaat gctaaaaata taactgtaac
     1981 tttcacattg aacaaaagta attttaaact tattgtagca gatgatggca atggttttga
     2041 cgtaactgat tttaataaaa taattagaaa tggaaacgga ttgattaata tgcaacaacg
     2101 aatgttccag catcaaaata aacttactat tatttcaact ccaggacatg gatgtaaaat
     2161 agttgcagaa gggaaacaga tttgatttca ttagtaatac tataatgtgt atatgaaaaa
     2221 aattaaaata attcttgttg aggatgataa agaaatacga gagttagcag aggcaattct
     2281 cggttctgaa aaagatatgg aagttataaa aacattttct aatggagaaa atttcttaaa
     2341 atcatgggaa aactttgact gtaatgttgt tgtgatggat ataaatatgc ccggcaaaac
     2401 cggtatagaa tgtgtagctg aagctaagcc taaaaaacca tcagtacaat ttctgattag
     2461 cacagttttt gaaaaccctc aatatatttt tcaagcctta tgtaatggag caacgggcta
     2521 tgtgctaaag aacaatacag gtgataagtt agtttctgca gttcgtgaaa ttgcatccgg
     2581 tggttcacct atgagtgcaa gtattgcacg tttagtggta gattcatttt cgcacaaaca
     2641 aacaactgta ataaataatg agattttaac ctcacgcgaa aaagaagtct tggatttgtt
     2701 agcacagggt ttgatgtata aagagattgc agccaaaaaa gaaatcgctg ttgaaacggt
     2761 gcgtaaacat gtttataata tttatgaaaa gctacaggtt gactcacgta cagcagcaat
     2821 caataaagtt tatggcaaac atccggaatc gtaaccttac attcttaata gcaagcaaca
     2881 acttaaagta ctgaatattt ttttatacct accacttaaa tggtatttac ttcgttttta
     2941 aacagatgta agtttgtaca gtagttataa tttgtaattg caatgacaaa caaatattct
     3001 ctctttttct cttttgtttt atttctaata cacattagaa tacaagcaca aaatgtaggt
     3061 attaacaacc ctacacccga tgcaagtgca ttgcttgata tggtttcgac tgacaaagga
     3121 attttaatac cacgcatgac aacgacattg cgtataacga ttgcagcacc ggctacaggc
     3181 ttattagtgt atgatagtga cctgaataag ttttactttt tcaatggaat tgcgtggatt
     3241 gctgtaggta gtaatattgg ttggagtttg actggagatg caggaacagc gtcaggaacc
     3301 aattttttag gtactactga taatcagcca ttattaatta aggtcaacaa tacaaaggct
     3361 gggtttattg atcaacaaga aggaaccttt cttggcagaa atgtgttttt aggcaagctt
     3421 gcaggcttca ataataccat tacaggtttt gacaatacat ttattggtgc taaagctggt
     3481 gaggcaaata caattggaac attaaacaca gcagtaggcg caaaatcttt acaaaacgca
     3541 gttgctactt tttcaaatac agccattggt cgtgaaacta tgcagcaatg catttcatgt
     3601 gtgcatagca cagctgttgg agccggtgca ttacaaaata ttgaaggtgg tttttggaac
     3661 aatgcttttg gctatttagc aatgaggcaa gctgctaatc ccgggataag taataatgct
     3721 tttggagccg aaactttgtt taataataca ggatctgata attgcgcatt cggacatact
     3781 gcattaagac aaaacacttc aggtacctac aatatagcat tcggcagctt gtctctttat
     3841 gaaaacaaca ccggaagtta taatattgga atgggagcat ttgcattgca aaataattca
     3901 acaggtagtt acaatactgt attgggctac aaatgtgctg cagacggact ttcaaataac
     3961 agagtagttg caattggcga cagtacctta tattttaata aagcttcagg tattgttgca
     4021 gttggttcac gggcattacg aagtaataat ggtggaacat ctaatacagc tgttggttat
     4081 gaaagtcttt atacaaacac cattggcaat tcaaacactg cattaggtga tttttcatta
     4141 aaactaaata ttgtaggaaa tgctaatacc gccataggaa gtttagcttt acaaaataat
     4201 aacggcaata ataatactgc agtaggttct ggagcacaat ctgctaatac tcttggtaag
     4261 gataatagtt ccgttggagc tttaacactt acaaataatc tgtcaggtaa ttttaatatt
     4321 gccattggaa gtaattcgct tgcacagaat atttcaagtc ataacaacgt aggtgttgga
     4381 tataatacac tatctgcata taacgcaagc ggaaacacag gtaataatac tgccatagga
     4441 accggggcag gcgatagcta tacatcatac tccaactgta cttttgtagg ttaccaggct
     4501 gatgcaaatg cagcaagctt tagcaattct acagctattg gtaatggagc aatagccaca
     4561 gcaagtcaac aagtcaaaat cggaaatgct acagtaacag ctattggagg cgcagttaac
     4621 tggagtatta tttctgatgg aagatttaaa accaatgtga aaaataatat tccgggacta
     4681 aattttataa atcggctaaa ccccgtatcg tataattatg atataaaatc ttattgtagc
     4741 tttattggaa ccgatgaaca ttcatttaat gatgatgcag caatacaaca aaaagaaaaa
     4801 attatttatt ccggcttcat tgcacaagaa gtagaagagg ctgctaagaa ttcagggtat
     4861 aattttagtg gaattcataa accggaaaat acgaatgata cctatacatt gagctatgca
     4921 gattttgttg taccattggt taaagctgtg caggaactga caattcaaaa tgttaatttg
     4981 ttaaaaaaga tagaattact ggaggctaaa tatgacttac ataaaaaaca agattaaagt
     5041 atctgataaa ccaagtcttg cttcattact atctgatttc atttcgctct ttacaaatga
     5101 catttataca atggaagcta ataggccaat tgacttggat agttatgcaa ctataattgt
     5161 attcttgaat aataaattag atgaaatctt caatggatag ccacaattcc caaaagtgtg
     5221 taaccttcct gaggaggttt gtataatacg tctcttgaaa aatggtttta gtaaaacaat
     5281 ctcattctaa agtgttattc aatacttctt tgtaaacatc aaaataagct ttctcattag
     5341 aagaaatatt aaaacgtgat gcatattgac ggttgttgtt ttgttcatca gctaaaacat
     5401 tcctattgtc ataatagaaa ttaagcctgt caacaagatg tgcagtactt tcaacaatat
     5461 taagtctatt taacggcttg atgacttctg taattcctcc tccatctgaa aaaactatca
     5521 ctgtcttacc tagcattaaa gcttcaattg ctacgatacc aaatgcctcg ttttcgaatg
     5581 ggaatacaca tacattcatt ttgttaaacc atgcaaaaac atttttttta aaacctgtaa
     5641 agacaacctg attttctaat ttttttatct tcacaatatt ctttaactcc tgcatcttta
     5701 caccatcacc tgtaaggagt aatgttgtat cctgtttgtt tttagaaaag atttcaaatg
     5761 cttctataag tctatcaatt cgtttattac ctgcaaaacg agaggctgtc cctacaataa
     5821 aatctgaaga ttttatcttt atcgctggat cgttttcatc ctgaatgggt aattcaatat
     5881 ttggaattgc attgtagatt agcgttgcag gtgttgattt ttttacttta taaaactcca
     5941 ttgcaatgtt acggcaaaat tcagagttgt aagtaatgtg atttacatga ttacggataa
     6001 aatatctgct tagaaattgt aatactttat cctttattgt acgcttgcgg ccaaacccaa
     6061 agttcccatg aacagtaaag actattttta tttttgattt aattgccgca taggctatag
     6121 ccgggttgaa agtatgaata tgcagcacat catatgatga catgagtttt gtgagtcggt
     6181 taattttact actggaaaaa tccagacctg atttcatatc agcaaattca cagtgcagac
     6241 ctgcctgctc tatcatttca agaaaatttc cctttctgat tcctatcagc aacgaaacct
     6301 ttatattttc atttaactgt tgttgttgcg caagagtgat taccaacttc tctataccac
     6361 caaaatttgc agaccaaata aaatgtaaca ctctcatttt ttaagatatt tagttaatca
     6421 taaacaagtc tcttctcctg tttaataatt aaaaaaacta aaattaataa ccgaatcaga
     6481 taaaaggcaa aagtaaactt tcactcgatt ttgttcaaca gaaattaaaa aagacaagta
     6541 gcgactttta ttaatcgaat gggtattgca gacgctcgtt taccatagat tgcttttata
     6601 ttttcaccac cattcattac aaccttattt ttgttgtaat tttcttcaaa gcacctctaa
     6661 aaatttaaac ttttttaaga gtgaaataaa gagtttttcg gaattgaata atgcggttgg
     6721 tattttctaa gaatatgaat tccttagctg ttataagccc atttaagata aactgctccc
     6781 caagtaaagc ctccgccaaa agcggcaaga atcaggttat cacccttgtg caattgtttt
     6841 tcccattccc ataaacagag tggtaatgtg ccattggttg tatttccata cttctgaatg
     6901 ttgagcatca ctttttccat gccaatgccc attcggttgg cagtagcatc aataattctt
     6961 ttgtttgcct gatgtggaac aagccatgca atatcatctg cactcaggtt gttacgttcc
     7021 ataatttctg cacttacatc agccatacct ttaacagcaa acttaaaaac cgtttgcccc
     7081 tcctgataga caaaatgttc tttggcatca atagttgcat gagttggagg tttcacacta
     7141 ccacctgctt tctgatgcag aaaatgtcgt ccactgccat cgctttttaa aacagcatcg
     7201 taaatgccgt aaccgttggt atccggttct aataatacag caccaccacc atcaccaaaa
     7261 ataatgcagg tggcacggtc gctgtagtca atgattgccg acattttatc ggcacccaca
     7321 acaacaactt ttttatactg tccgctttca acaaattttg aagcagtagt caatgcaaat
     7381 aaaaatcctg aacaggctgc ctgaatatcg tagccaaaag catttttagc acctacttta
     7441 tcacaaataa tatttgctgt ggccggaaaa accaaatcag gagtagtagt ggcacaaatc
     7501 agcaagtcaa tttcttctgc ctgtatgcca cgctttttta atagcccgtt taccgattca
     7561 acaccaattt tggaagtacc ttcaccggca tcttttaata ttctgcgttc cttaattcct
     7621 gttcttgttt gaatccactc atcgttggtt tcaaccatgg tctccagttc tttgtttgta
     7681 agaacatagt ccggcaccca ggcatgaaca gcggtgatgg cagctctgat tttttccatc
     7741 aaaaaaaatt atagtgtttg attaactacg ttgttttgaa aaaaagttgc gattttttct
     7801 gcaagatggg cttcggtaac ttctttggca agcaatatca tgtttttgat ggccttcgat
     7861 tttgaaatgc catgtgcaat aattacattt ccgtttacac caagcaccgg tgtgccacca
     7921 taattttcat aatcaaagcg gtcgaagtat gcatctttaa taccgcgttt tttaagtagc
     7981 gtataaagtg attcggcttc tttaagcatt acattacctg tgaagccatc gcatacaatg
     8041 acatcagctt tgttgttgaa caagtcgcgc ccttcaacgt taccgataaa atttatttct
     8101 ttagtgtttt taagcaactg atgtgcctgt tgcgaaagca ggtttccttt ttcttcctct
     8161 tcaccaatat tcatcaaagc cacacgtggt gatgcaatat gataaacatg ttctgcaagc
     8221 agcgaaccga gtaatccaaa ttgttgcaac acttcgggct tacagtcggc attggcacca
     8281 acatcaagta aaattcctgt tgagccgtct tcctgtggta aaatggttga gatagcagga
     8341 cgctgaatac cattaatggc tttaacgctg aacatgctgc caaccaacat agcaccggaa
     8401 tttccggcac tggcaaaggc atcaatcttg ttttgtttta gcaggccaaa accaatggcc
     8461 atacctgagt tgggtttctg agagaaagca cgtgttgcac tgtcagacat ttcaatcacc
     8521 tctgtagcgt ttacaattgt aaacgactta gggttgactc cctgcccttc aattatctct
     8581 gttatttttt tctcatcacc tattagaacc aggtgtactt cctgaggcga aaggtcattt
     8641 aatgctgcaa ttgcacctaa ggtagtttcg agtggagcat aatctccacc cataatatca
     8701 actcctatcc ttatcataaa ggaatacagc gattaaacag ctttttctaa aaccaccttg
     8761 cctttgtagt acattttgcc ctcatgccag taggcacgat gatataaatg tgtttcgcct
     8821 gtggtaggac aaatggcaat tgttggagct tctgccttgt aatgtgttct gcgcttatct
     8881 cttctcgact tggagtgttt gtgttttgga tttggcatga cgtttaatta tttaatatca
     8941 attgttactt acatttttta aaatatccca tcgcgggtcg tttgtgtttt cattcttcac
     9001 catatacttt tcaagtgtac gaagggtttc ttcattgcag ccattgtcag gatgtgtcac
     9061 ttttatgggt atagctaatg aaactacatc ataaaaatgc tgtgccagat gaatatcgtg
     9121 ggcattgtcg gctaaaatga tgttgtcatc atccacttct ttgtcttcgc cagaggtttt
     9181 aacgataata gccttgctta tatccaaagg catctcaaac tcttcaagac aacgatggca
     9241 ctgtacgtgt acggtgcctt tagctttaat ttccagaacc acaatggtgg tgcttttgtt
     9301 tacggtaaga tgaataagaa catgtgctcc ctgaacaata gggtgttcaa acatgtcgaa
     9361 aaagtcctga ttcagtaagt aatcaaactc atgtatcccc tcttttaaac cggcataatg
     9421 caggttatat ttcgacatct tttccactac gtacttccta ttttgggaac gaatgggcaa
     9481 aagtataaaa aatcgctatt ccaacacctt gtttttgtta attattatta agggctattg
     9541 attgtcagga gatagaacca tttaagtatt aacaaaatat gtcggtaaag attggtttac
     9601 tgcatgtaat tgacggtatt ttgcagtagg aattggcaat atcttctgga aataaaacaa
     9661 atattttcca ttaacatatt caaattacca ttggtacaaa aaagcatggt aaaataccca
     9721 attaatgtta acattgttga gtcaagcatt ttttaagccg aaaatgattg tatagtcaga
     9781 agtaaaaacc actataatta aacaggctca aaatttccga agcatggatc ttttgttcta
     9841 cacacacgaa agtaaattat atggattaac catctttgtt tctatagtta cgttccagat
     9901 tgtttatgtt tttgtgcaat ggcttatcaa acaccgaagg gattatctgt tttatattgc
     9961 ctatttgttt tttgtcattg tttttggatt aagccactac gacaagtatt taaactttta
    10021 tcctttttca ggaattgcag agcgatatta tgagtatata aacctatcag caccgctgct
    10081 ttgcattctt ctttacttca gatttatgcg tgagtttttg aacttaaaac acaggcgacc
    10141 tgatattaat acaatggtaa agaaccttga gttttttctg attgcttaca cactacttag
    10201 cccgtttttt atctggcttt cacttgatat tgctgtctgg aaaatgatct ttaatgttgt
    10261 tgcaataatt acaatcactg cgggattatt tataataatt aactttttaa agaagagtat
    10321 tccactcgac agatttgcta tgacaggtgc gtcattaatt gctgtgggtt caatatttga
    10381 tattgcactt agctttcttg agttcagagg atttaatatc ccctttgaca tacacctgcc
    10441 attgatggtt tgtgttattg gggaactcac aacttttact ctgggacttg catttaaaac
    10501 acagtttgtc gaaaatgaaa aactatttgt agaaaaaaac tttctttccg aaaaaagtga
    10561 aaaacaacag ttacagttag acattttaaa gcttagaaat aatctggcac gcgaactcca
    10621 cgatgatatt ggtgcacaac tatcacttgc cagaatgttg atgagtaagg cacgtgctga
    10681 tggtaatgaa agggtagtag atcaatctat gcaactgctc gaagagtcaa taaacaaact
    10741 gcgttctgtt actaacgaat tagaaaatac tactttacag aatgaaggtt ttcagaaagc
    10801 aacagaaaaa ttgttgagca ctatttcgga gtttcagcct attcattgta acttctatgc
    10861 aaaaggtgtt gaaaaacgat tgcatcttga tgttgaatat catttattcc gcattactca
    10921 agagctaatc aataacactc taaaatatgc ccatgcttct attataagca tcattgtttt
    10981 aggcgatagg gataagtttt attttatcta tgaagacaat ggtttgggct ttgatttaaa
    11041 aaactcaggt cgtggcaagg gcttaaataa tattttgcag cgcgtaaaag aattaggcac
    11101 caagacagag tttattgctc aacccggtgg tggcatgcgc tgtgaattca aaattgacta
    11161 tgaacaatca atgcaaatag cctgattatt taatatggca ctaagtaact taaaaatttt
    11221 gattgctgat gaccataagc tttttgccga aggcctcacc tatcttttaa agcaggagtt
    11281 accaatagaa agtattgaca cggtttataa cgggaaatca gccattggaa aagtgttgaa
    11341 tacagattat gatattatta ttatggatgt taatatgcct ttattaaacg gcatagaaac
    11401 ctgtgcagcg ataaaaagat tcagaacaca aaccaaaata gtttttgtga gcatgaaagc
    11461 cgatatgcta acagttgcac aggcattaaa agcaggtgcc gatgcataca tattaaaagg
    11521 taacgatcat aatgatttta taactgcatt taaacagatt aacaacaatg agatttttct
    11581 aagtgaacaa attaaacatt actttcagga tgataatcct aatcaaatcc gtaaaaatgc
    11641 aattgcctgt gtagattatt ccaacagtct gatttctgtt cgcgaaaaag aaattattaa
    11701 attagtttgt gatggattaa cgagtgataa aattgctgaa gtactttcta tctcaaccag
    11761 aacggttgat acacacagaa acaatatact caataagcta aaactaaaca acattgcctc
    11821 attggttcgc tttgccttag agaataagct ggtggattaa tttcttctgc aagaaataaa
    11881 atcagcactt aattctaata attaaaaatt acgaagtttt acgtattgca ttcagtagtt
    11941 tgcgggctta cttttacact atcaatttca caacaacaaa tacaaaggga gttagaagtt
    12001 caaatacaaa tcatcaaaat aaacaacatg atataatttt tacactgatt tataaaacaa
    12061 caaaagttga attaattaag gggacagttg ttctccaaca aaaatagtta accaacaaaa
    12121 tgtctaacag ttaaaaatta gtcaagatga aaaagctgat tattgtatta agtgtagcag
    12181 caaccgcggc aattatcggt tgcagcaagg acgatctcac cagcccacag gctctgcaaa
    12241 acaaaaacac cacaacagca acaaatgatg ttagtgcaac agatgaacgc ctcatcaatt
    12301 ccggcactgt attggtgcct gcgcccaacc aaggttatgt caaagtgttt ctttttggtg
    12361 cggcaaaact ctctgccgac aaacccgaaa catttgttga catcaacaaa agagatgtat
    12421 cgcaacgagc attcgatgcc agcttcaaag ctatgtatgt aaaattcaat gagcgttgga
    12481 tatatgttcc aggaataatt ccaaactatg gcatagaagg taaactaact tatagagttt
    12541 catcatcaca agccagcgac cctgacgcta caaaccaaat tcttgcatgg catcggttta
    12601 cactcaccat tcgcaactta aacaacagac catatacagg caggttggta tctttaagca
    12661 acataaaagt tttgataata cctgcaaaga gcgaacccgg tggtcatgac cctgatttaa
    12721 gaaactatga agccaccatg acccattttg gcttaccgct tgattaatgt tgaagttttc
    12781 taaacaaagg aggcagctgc attcgcagcc gcctcgtttg ttttattgct attggctcac
    12841 ttagaaatta tatccgtccg attttatcct attatcattc ttacctttat ttttgttcag
    12901 actaaacctt ctttacaata tcgtgtcaaa tacacagttt gttttgttat gactgaaaac
    12961 gaaaactttc aaaaaataaa gcacctttat aaccgtgccg gttttggatt acactattct
    13021 gagctgcaga aactaagccg cacaaagctg aataagttag ttgccgatat gattgaaaaa
    13081 gcaaagaccg atgtaccaat tacaaccatt gctcccggcc atgtaaaatc tgtgatgctt
    13141 aaaaatgcag gacttaccaa gaaagaaggc ttgtcaccgg agcaactaaa agaaaggcaa
    13201 aaacttttag tgagccttaa caaacaactt aaaaacctga atacagaatg gttcttccgc
    13261 atgatggata ttgacagccc actacgcgaa aaaatgacct tattctggaa caatcacttt
    13321 gcatgtcgcg aagaagggaa tccatatttt gcacaagtgc tcaataacat tcagcgcaaa
    13381 catgcattag gtgatttcag gcagttgtta ttggaagtat ctcagtcggc atctatgtta
    13441 aattttttaa ataatcagca aaataaaaaa ggcagaccta atgaaaactt tgcacgcgaa
    13501 ctgatggagt tgtttacact tggtcgtggt aactattcag aaaaagatat taaagaatca
    13561 gcacgtgcat ttactggttg ggtgcacgat gcagaaggaa attttgtgtt caacaccaag
    13621 aatcacgaca atggcataaa aacatttttc ggaaaagaag gcaacttcaa cggtgaagat
    13681 attattgata tgattttgca aaagcctgag gctgctgtat ttattgcaag aaaggcttat
    13741 cgcttttttg ttaacgatgt gcctgatgaa acacatgtgc aggaattggg taaccatttt
    13801 tataaaagca agtacgatat ttctgtactg atgaataaaa tgtttactgc cgattggttt
    13861 tatgctcctg aaaacacagg taataaaatt aaatctccgg tagagtttat agttgggctt
    13921 agtcgtcagt ttaatgttac ttacaaaagg caggaaatta ttttacaact gcaaagaggt
    13981 ttggggcaaa cattatttta tcctcctaat gttgcaggtt gggcaggcgg tcgaaactgg
    14041 attgacagct catcattgat gttgcgatta acaattcctt ctcagattct taatgatgga
    14101 agtttaaatt tttctgctaa aaccgatcct gacgatgaaa acattgtgg
//
LOCUS       DAOOOI010000055        13845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_881_length_13845_cov_19.482814, whole genome shotgun sequence.
ACCESSION   DAOOOI010000055 DAOOOI010000000
VERSION     DAOOOI010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13845
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_881_length_13845_cov_19.482814"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PKX59_07665"
     CDS             complement(<1..638)
                     /locus_tag="PKX59_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HOZ90781.1"
                     /translation="MAIILGISAFYHDSAATIAIDGKIISAVQEERFSRIKNDASFPV
                     NAIKFCLLHSGLKLNQIDHIVYYEKPFLKLERLLETFLETAPKGLQQFTQAAPIWIKE
                     KVLIKNKIRESLHLIDENYSAKEILFCEHHLSHAASAFFTSPFPDAAIITTDGVGEWC
                     TTSIMNGKLNSITSIKELNFPHSIGLLYSAFTQFLGFKVNSGEYKVMGLAPYG"
     gene            complement(672..2552)
                     /locus_tag="PKX59_07670"
     CDS             complement(672..2552)
                     /locus_tag="PKX59_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ90782.1"
                     /translation="MIRKRKPYLLFVVLIFTSCLSVKQRTADTYYEQNLYDKAIPYYQ
                     EVANKKFNLDILIKLADCYRNVKNYPLSEQIYSKIVTSNSDPIYQFYYAEALMQNGKY
                     VEARKWLNKFLVLNRADSRALNLLASCDSIEMLYKDSLLFDVTRLPFNGIDESNFSPE
                     FFHSGIVYVSNRNIDAKSYKTNPVIDNYNLFYAQQTELGHWMDPVPLRGTINSIFNEG
                     PCTFNTEFTKAYFTRNANEGKKIITNQQSENVLEIFEATTKDGEWNISGPVSFNNNNY
                     SVGHPSISPSGNTMVFVSDMPWGYGGTDIYKVQWVNNTWSKPVSIGKTINSSGNEMFP
                     YLLTDSILYFSSNGMIGVGGLDIYRTELTADGWTVPENLGYPINSPRDDFGLICDNEE
                     LSGYFSSNRQQGKDNIFSFKRNPPVLTLKGKLIDKSTNKSLKNINITLTASSIDTVLT
                     TDSEGNFSAQLKNNKEYKLRAEDKSYFTASEKISTFGYRKSATLNKDLTLEKVIFNKP
                     VIWRGIAFEKGKSDLTPSTKTELNKLFNILLENKNIKIELSCHTDSRGIERDNLILSQ
                     ARADKAAAYLINKGISKDRIISVGFGSQRLLNNCVRGVLCLEEDHQVNIRTEIRYSPY
                     QR"
     gene            complement(2555..3475)
                     /locus_tag="PKX59_07675"
     CDS             complement(2555..3475)
                     /locus_tag="PKX59_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOZ90783.1"
                     /translation="MKKLYSLIITLFMSGWLFGQQAPMTTQYMFNTLLINPAYAGSKP
                     FTSATMMVRKQWAGFKGSPTTETASIHGALEDKNVGLGLYISNDHAGITNRTELFGSY
                     SYTIEYGESKLAFGLQAGLSYLKSTLSDLVYNDNNDPVYDKNTVNNVLPNFGAGLYYH
                     TDLFYAGISAPLLLSYDPLKTLSVEMTDVHHIRRHYFLTTGYVFSLSEQVKLKPSVLL
                     KYVNGAPLQYDINANLLLNNIIWIGTSYRSNDALAIIGEYQLSKKLRIGYSFDYSLTI
                     LRNHSAGSHEFMLGYDFGYNVPKIKTPRYF"
     gene            complement(3480..12242)
                     /locus_tag="PKX59_07680"
     CDS             complement(3480..12242)
                     /locus_tag="PKX59_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOZ90784.1"
                     /translation="MSTTKSFAQPVLSASFSNYNGYNISCNGLSDGQIDLTITGSGPF
                     NIQWSNGATTEDISGITAGSYTVTVVDVANDTTIETYSLTEPDAITRSAVSIQDVTCF
                     GLNNGSINISVDGGVPNYSFQWSNGSGTEDISGLIAGVYTLTITDLNNCTAVFANDTV
                     HEPDEIKVTATVTNTSCGLINGMIDASISGGISPYGYLWSNSNTTEDLNILASGVYTL
                     TVTDQNLCTKVDSFIVGASTQPVVVLDSAFNVTCFGQSTGRIYVHVNSANGPINYLWS
                     NASVSQNLLNIPLGNYTVTITDSTGCQSSLSHTITEPTDIAVTLTPHNATCAQSNGWI
                     SSAVTGGTSPYTYLWSNGASTSSITGLLPGNYTVTVTDAALCTKTASQNISTISGPVV
                     TVDSVKNARCFGQANGAVYISVSGNNGPVTYLWSNAAITQDIVNIVAGTYTVTVTDSS
                     GCTATKTQAITQPIDIAVTLTPHNATCAQSNGWISSAVTGGTSPYTYLWSNGASTSSI
                     TGLLPGNYTVTVTDAALCTKTASQNITTISGPVVTVDSVKNARCFGQANGAVYISVSG
                     NNGPVTYLWSNAAITQDIVNIVAGTYTVTVTDSSGCTATKTQAITQPIDIAVTLTPHN
                     ATCAQSNGWISSAVTGGTSPYTYLWSNGASTSSITGLLPGNYTVTVTDAALCTKTASQ
                     NISTISGPVVTVDSVKNARCFGQANGAVYISISGNNGPVTYLWSNAAITQDIVNIVAG
                     TYTVTVTDSTGCVGNKTQVVSQPTNIAVTLTPHNATCAQSNGWISSAVIGGTSPYTYL
                     WSNGASTSSITGLLPGNYTVTVTDAALCTKTASQNITTISGPVVTVDSVKNARCFGQA
                     NGAVYISVSGNNGPVTYLWSNAAITQDIVNVVAGTYTVTVTDSSGCTATKTQVVSQPT
                     DIAVTLTPHNATCAQSNGWISSAVTGGTSPYTYLWSNGASTSSITGLLPGNYTVTVTD
                     AALCTKTASQNITTISGPVVTVDSVKNARCFGQANGAVYISVSGNNGPVTYLWSNAAI
                     TQDIVNVVAGTYTVTVTDSTGCVGNKTQAITQPTNIAVTLTPHNATCAQSNGWISSAV
                     SGGTSPYTYLWSNGASTSSITGLLPGNYTVTVTDAALCTKTASQNITTISGPVVTVDS
                     VKNVRCFGQANGAVYISVSGNNGPVTYLWSNAAITQDIVNVVAGTYTVTVTDSTGCVG
                     NKTQVVSQPTNIVVTLTPHNATCAQSNGWISSAVTGGTSPYTYLWSNGASTSSITGLL
                     PGNYTVTVTDAALCTKTASQNITTISGPVVTVDSVKNARCFGQANGAVYISVSGNNGP
                     VTYLWSNAAITQDIVNVVAGTYTVTVTDSTGCVGNKTQVVSQPTDIAVTLTPHNATCA
                     QSNGWISSAVTGGTSPYTYLWSNGASTSSITGLLPGNYTVTVTDAALCTKTASQNIST
                     ISGPVVTVDSVKNARCFGQANGAVYISVSGNNGPVIYLWSNAAITQDIVNIVAGTYTV
                     TVTDSTGCVGNKTQIITQPSALTLIDTIVPARCGNANGSAGVNVTGGTSPYTYLWNTG
                     QSTASISGLNGGSYTVTVTDSKLCTANRTLIVSNTPSLVISIDTIIHVKCSGQNTGAI
                     KVNVTGGSGPLTYTWTPTQIGTDSIFSLAAGTYKLVVRDTANCRDSINVTINSPSAIN
                     SAIYSLPETCGINNGKAWVTVSGGTPGYTFLWNNSSTNDTISNLTKGTYSVTIKDANN
                     CSVNKNILVDSLGGPAITDSIIHVKCFGENTGAIYTTVHGANGPVSYLWSPGGQITQN
                     INGLTAGTYTVTVTDTNSCSSTKSFIISQPTSLNISFTSVTEKCNQANGSAIAAVIGG
                     TLPYSYQWNNGNTTNNIINLTTGYYTITITDGNLCTKRDSVFIGNTNGPSISNFSRNL
                     IKCHNDSSGSLTVNVTGGTPFYNYQWSGFPGVNNATLQNIPAGVYTVTVTDANGCSVN
                     ATDSLQNPPLMTITHTLSNASCNLNNGIAQINVSGGAAPYTYSWSNGVTGVATLNNLS
                     NIFYTVTITDNIGCIKTDTFTIQRTPATFVQLTSLNNVTCYGLSNGSITITPSGGDGN
                     YTYQWSYNNIQTQNLNNIPAGSYDVTVTDGTGCTYTMPDIQVFQPDTLIAQVITLPTG
                     CTSQTGSASVSQTGGTQPYTYLWSTGGTTQTINGLSSQIVTVTVTDNNGCTTTTSGII
                     NALSAPVINISGIQNINCFGGGNGFINTTVTGGNLPYNYLWNNGSTTSNISNLSAGTY
                     TLTVTDSAQCSTIITQTLTENPQIQFGLTLIAGPCNGGTPAIAISAPSGGVSPLNILW
                     SDGSTSTSAQATAGTVMTLTVTDNAGCNRDTTFSMLSTNGPQISSIQTTDIICFGDSS
                     GSATVNITGGTPPISIQWIGTLPVQNTATAINLKAGTYTCIVTDVNGCSAQSVANINH
                     LNTLITPVLAITNASCGNSNGQIVVTATGGSGTYQYKLNNGVYSATNTFTNLAAGSDS
                     VFVKDNNGCEVFVISTISNIQGPVISVIDSANVSCFGSNDGFIILNVTGAAQPITYSW
                     SDGPTTLTRNNLGPGNYTFTAQDANNCISVRNFIITEPTAITISEIIPNKNGIYNITC
                     NTLADGEIQVIANGGVPFTSTTAYNYQWSISGASGANVINLTAGPYTVTVTDSMGCSV
                     TKSFLLTEPPALNAGPDFTQSICGTDTIVLTGNIPTYGFGKWTVRSGGGLIAEPDSIT
                     TQITAIANGVNVFAWVVYDNFCSDTALYVINKKEKIIADGGIDQNVCDNFVFLAAVPP
                     PSGTGVWQSINGNSTIQNPSSPATKVENLSPGKNIFVWTITNGSCTASDTVKIAILPP
                     EECYTDVVMPTGFTPNEDGYNDIYVIRGLDNTDNTLTIFNRWGNLVYEKKNYSNDWDG
                     KSNNKQILPDGTYYAILRIPARNLVLKTYIDLRR"
     gene            12482..12988
                     /locus_tag="PKX59_07685"
     CDS             12482..12988
                     /locus_tag="PKX59_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90785.1"
                     /translation="MHNFRTKGTTSYTMNSLKSIITISILTILLIFVSADGQSQPVNN
                     ASTKPRQVDVVICMDLSGSTNGLLDNFRDKMWDIINQWQMFTPKPVIRIGLIGFSRYS
                     FHDGSGYIKIISDLTDDYDALSQKMFDIQPYVENGIQFVGAAIGSGVNDMSWSEKPDA
                     VKMMFVIG"
     gene            13095..13682
                     /locus_tag="PKX59_07690"
     CDS             13095..13682
                     /locus_tag="PKX59_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90786.1"
                     /translation="MRQRELPGWEEIAKMTGGQSFQVFVNNRAPLIKEKVNVMALASL
                     NDSLNKTFIPYGKTGVDRLKLMLKADDHAVRSYEAYFYSRLKYKLSDHYTSQMLMWDV
                     TQYKEEYPQSRLAAVRQSVKSNNESSVDLELLAKEFVEKRNSLRASINAMFPLGEELR
                     VHNEAVAEEYELDNRLDKTMVRAYSQTLLKFGFKE"
BASE COUNT         4219 a         2547 c         2660 g         4419 t
ORIGIN      
        1 ccataaggtg ccaagcccat tactttatat tctcctgaat taactttaaa accaaggaac
       61 tgtgtaaatg cactatataa taatcctatt gaatgtggaa agttaagttc ttttatagag
      121 gtaatagaat ttagtttgcc attcataatt gaagtagtgc accattctcc aacaccatct
      181 gtagttatta ttgctgcatc cggaaaagga gaggtaaaga aagcacttgc agcatgagat
      241 agatgatgct cgcagaatag aatttctttt gctgaataat tctcatctat caaatgcaaa
      301 ctttctctga ttttattttt tatcagaact ttttctttta tccatattgg tgcagcctgt
      361 gtaaattgtt gcaagccttt aggtgctgtt tcaagaaaag tttccaataa cctctctaac
      421 ttcaaaaaag gtttttcgta atatacaata tgatcaattt gatttaactt taaaccggaa
      481 tgcagcaagc aaaattttat tgcattgaca ggaaaagagg catcattttt aattcttgaa
      541 aagcgttctt cctgtactgc tgagattatt ttaccatcaa tagcaatagt tgctgccgag
      601 tcgtgataaa atgcggaaat gcctaatatg atggccatct ttgcagaaaa atatgttctg
      661 tctattaagt cttatcgctg ataaggactg tatcttattt cagttctgat attaacctga
      721 tggtcttctt caagacataa tacacccctt acacaattgt ttaataatcg ttgggatcca
      781 aatccaactg aaataattct gtcttttgag attcctttat tgataagata tgcagcagct
      841 ttatctgctc ttgcctgtga taatatcagg ttatcacgtt ctatgcctct tgagtctgtg
      901 tgacagctca attcaatttt tatattttta ttctctaata aaatgttaaa cagtttattt
      961 aattctgttt tagttgacgg tgttaaatct gattttcctt tttcaaatgc aatgcctctc
     1021 catataactg gcttgttgaa aatcactttt tccaaagtta aatccttatt cagtgttgct
     1081 gattttctat aaccaaacgt agatatcttt tctgatgctg taaaataact tttatcctca
     1141 gcacgaagtt tgtattcttt attgtttttt aattgcgcag aaaaattgcc ttcgctatct
     1201 gttgttaaaa cagtatcaat tgatgaagca gttaacgtaa tattgatgtt ttttagagac
     1261 ttgtttgttg acttgtcaat caatttgcct tttaacgtta aaaccggagg atttctttta
     1321 aatgaaaaaa tattgtcttt tccttgttgc ctgttactac tgaaataacc acttaattct
     1381 tcattatcgc aaatcaaacc aaaatcgtct cttggactgt taatcggata gcctaggttt
     1441 tctggtacag tccatccatc agcagttaat tctgttcggt aaatgtccag acctccaaca
     1501 ccaatcatac cgttgcttga aaagtaaagt attgaatcag tcagtaagta aggaaacatt
     1561 tcatttccac ttgaattaat tgtcttgcca atactaaccg gtttactcca agtattatta
     1621 acccattgta ctttataaat atccgtaccc ccataacccc atggcatatc agaaacaaag
     1681 accattgtgt tgccactggg tgaaatagat ggatgcccta cactgtaatt gttattgttg
     1741 aaactaacag gccctgaaat attccattca ccatctttgg ttgtagcttc aaaaatttct
     1801 aaaacatttt cactttgctg attagtaata atttttttcc cttcattggc attgcgtgta
     1861 aagtaagctt tagtaaattc agtgttgaat gtgcatggcc cttcattaaa aatagaatta
     1921 attgtgcccc gtaaaggaac cggatccatc cagtgaccta attcagtttg ttgtgcataa
     1981 aataaattgt aattatcaat taccggattt gtcttataac tttttgcatc aatatttctg
     2041 ttacttacat aaacaattcc ggaatgaaaa aattcgggtg agaaattgct ttcatcaatt
     2101 ccgttaaaag gcaatcttgt aacatcaaag agcaatgagt ccttgtatag catttcaata
     2161 gaatcgcatg atgctaataa gtttaacgct ctgctgtctg cacgatttaa aacaagaaat
     2221 ttgtttaacc attttcgggc ttcaacatat ttgccatttt gcattaatgc ttcagcataa
     2281 taaaattgat atatcgggtc actatttgaa gttactatct tcgaataaat ctgttctgat
     2341 aatggatagt tctttacatt gcgataacaa tctgcaagtt taatgaggat atctaaatta
     2401 aactttttgt ttgcaacttc ctgataatac ggaatcgctt tgtcatataa gttctgctca
     2461 taataggtgt ctgctgttct ttgtttgaca gataagcatg atgtgaatat cagaactaca
     2521 aataataaat agggcttgcg ttttctaatc ataattaaaa ataacgcggt gttttaattt
     2581 taggtacgtt ataaccaaag tcgtatccaa gcataaattc atgcgatcct gccgaatggt
     2641 ttctgagaat ggtaagcgaa taatcaaagg aataaccaat gcgtagtttt ttagatagct
     2701 gatattctcc aataattgca agtgcatcat tggagcggta tgaagttcct atccagatga
     2761 tattgttcaa aagcagattt gcattaatgt catactgtaa tggggcacca ttaacatatt
     2821 ttagcagaac agaaggtttt aatttcacct gctcacttaa tgaaaaaaca taaccggttg
     2881 ttagaaaata gtgtcttctg atatgatgta catcggtcat ttcaactgac aaagttttta
     2941 gtggatcgta acttaaaagt aatggtgcag aaatgcctgc atagaatagg tcggtgtgat
     3001 aatatagacc tgcgccaaaa tttggcaaca cgttatttac agtattctta tcatatacag
     3061 ggtcgttgtt gtcgttataa acaagatcag atagtgtgga tttcagataa ctcaaacctg
     3121 cctgaagacc gaatgcaagt tttgattctc cgtattcaat agtataagaa taacttccaa
     3181 agagttctgt gcgatttgta atgcctgcat gatcattact tatatacaag cctaagccaa
     3241 cgtttttatc ttccaacgca ccatggatac tggcagtttc tgttgtcggt gatcctttaa
     3301 atcctgccca ttgttttcta accatcattg ttgcactggt gaatggttta cttcctgcat
     3361 aagccggatt tatcagcagt gtattaaaca tatattgtgt tgtcattggt gcttgttggc
     3421 caaacaacca gcctgacata aagagtgtaa ttattaatga atataatttt ttcataagct
     3481 tatcttctta aatcaatata tgtttttaag actaagtttc tggcaggtat ccgtaaaatg
     3541 gcatagtaag ttccatcagg taagatttgt ttgttgttag atttaccatc ccagtcgtta
     3601 ctatagtttt tcttttcata aactaaattg ccccatctgt taaaaatggt caaagtatta
     3661 tctgtattat ctaatcctct gataacgtat atatcgttat aaccatcctc attcggtgta
     3721 aaaccagttg gcataacaac atcagtataa cattcttccg gaggaagaat ggcaatcttt
     3781 actgtatcgg atgcagtaca tgaaccattt gttattgtcc aaacaaaaat atttttaccc
     3841 ggacttaaat tttcaacctt agtcgcaggt gatgatggat tttgaatagt actatttcca
     3901 ttgatgcttt gccatacacc agtacctgaa ggtggtggta ctgctgcaag gaatacaaaa
     3961 ttgtcgcaca cattttgatc aatgccacca tctgctataa ttttttcttt tttgttgatg
     4021 acatataatg cagtgtctga acagaaattg tcatagacta cccatgcaaa aacatttacc
     4081 ccatttgcaa ttgcagtaat ctgtgttgtg attgaatccg gttcagcaat taatccacct
     4141 ccactacgaa cagtccattt tccaaatcca taagtaggga tatttcctgt aagtacaatt
     4201 gtatcagtac cacaaataga ttgtgtaaaa tcagggcctg catttaaggc aggaggctca
     4261 gttaatagga atgattttgt aacagaacat cccatagaat ctgttacagt tacagtataa
     4321 ggtcctgcag taagatttat cacattagct cctgaagcgc cactgattga ccattgatag
     4381 ttgtaagcag ttgttgacgt aaatggaaca cctccatttg caatgacctg aatttctccg
     4441 tcagcaagtg tgttgcaagt tatattgtaa ataccgtttt tatttggaat tatttcactg
     4501 attgtaatgg ctgtaggttc tgtaatgata aagtttctta ccgatataca attgtttgcg
     4561 tcctgcgctg taaatgtata gttgcctggt ccaagattat ttcttgttaa agttgttggt
     4621 ccatcagacc atgaataggt tataggctgt gctgcaccgg taacatttaa aataatgaaa
     4681 ccatcattgc tgccaaagca gcttacatta gccgagtcaa taactgatat taccggccct
     4741 tgtatattac ttatagtcga aataacaaat acttcacagc cattgttgtc tttaacaaaa
     4801 accgaatcag atcctgctgc gaggttagta aatgtatttg ttgcagaata gactccatta
     4861 ttaagtttgt actgataggt accactacca ccggttgcag ttacaacaat ctgtccgttg
     4921 ctgtttccac aacttgcatt tgttatagca agcactggag ttatcaatgt attcaggtga
     4981 ttgatatttg caacactttg tgctgaacaa ccatttacgt ctgttacaat acaggtataa
     5041 gttccggctt ttaaatttat agccgttgca gtattttgaa ccggtaaagt gcctatccat
     5101 tgaatgctta ttggaggtgt tcctcctgta atattgactg ttgcgctgcc gctactgtct
     5161 ccaaaacaaa tgatgtctgt cgtttgaata cttgaaattt gcgggccgtt ggttgataac
     5221 atactgaatg tagtatctct attacatcct gcattgtcgg taactgttaa ggtcattact
     5281 gttcctgccg tagcttgtgc agatgtggaa gtgctgccat ctgaccacaa tatatttaat
     5341 ggcgatactc cacctgaagg tgctgatatg gcgattgcag gagttcctcc gttacatggg
     5401 cctgcaatta aagttaatcc aaactgaatt tgtggatttt ctgtaagagt ttgagtaata
     5461 atggtactac actgcgcaga gtccgtcacc gttagtgtgt aggttcctgc tgaaaggttg
     5521 ctgatatttg acgttgttga gccgttattc cagaggtaat tgtatggtag atttccccct
     5581 gttacagttg tgtttataaa accattccca cctccaaaac aattgatgtt ttgaataccg
     5641 gaaatattta taacaggagc tgataatgca ttgataattc cggaggttgt tgtcgtacat
     5701 ccattattat cagtcaccgt aacagttaca atctgacttg ataatccatt aatagtttgt
     5761 gtggtgccgc ctgttgacca taaataggtg taaggttgag taccacctgt ctgacttaca
     5821 ctggcacttc ctgtttgtga agtacagccc gttggtaatg tgataacttg tgcaattaaa
     5881 gtatctggct ggaaaacctg aatatccggc attgtgtaag tacatcctgt tccatcagtc
     5941 actgtaacat catacgagcc tgcaggaatg ttatttaagt tctgagtttg tatattgttg
     6001 tatgaccatt gataagtata atttccatcg cctcctgaag gtgtgatggt tatacttcca
     6061 ttactaagtc catagcaagt aacattattt aatgatgtaa gctgtacaaa tgttgcggga
     6121 gttcgttgta tagtaaatgt atctgtctta atacaaccaa tattgtctgt gatagttacg
     6181 gtataaaaaa tattagataa gttatttaaa gttgcaactc cggttactcc attactccaa
     6241 ctgtaagtgt acggtgcagc acctccggaa acattaatct gtgcaatacc gttatttaaa
     6301 ttacaagagg cattggagag tgtatgagtg attgtcatta aaggaggatt ctgaagactg
     6361 tcggttgcat ttactgaaca accattagca tcagttacag ttactgtata aactcctgca
     6421 ggtatattct gtagtgttgc attattaaca ccggggaatc cactccattg atagttgtaa
     6481 aatggtgtgc cgccagtaac attaacagtc agtgaaccac tgctatcatt atgacatttt
     6541 attaaattac gactgaaatt tgatattgat gggccatttg tatttccgat aaaaacagaa
     6601 tctctttttg tacacagatt tccgtcagtt attgtaattg tgtaatatcc cgttgttaaa
     6661 ttgatgatgt tatttgtagt attaccattg ttccattggt aggagtaagg tagtgttcct
     6721 ccaattactg cggcaatggc agaaccgttt gcctgattgc atttttcagt tactgaggta
     6781 aaagaaatat tcaaacttgt tggttggcta ataataaaac ttttggttga agagcaacta
     6841 ttggtgtctg taactgtaac agtataagtg cctgctgtca gaccgtttat gttttgtgtg
     6901 atttgaccac caggagacca taaataacta acgggaccat tggctccatg aacagtcgta
     6961 tagattgctc cggtgttttc tccaaaacat tttacatgaa ttattgaatc agtaattgcc
     7021 ggtccaccaa gtgaatcaac caatatgttt ttattgacac tacagttgtt tgcatctttt
     7081 atagtgacag aataagtgcc ttttgttaaa ttcgaaatgg tgtcattggt tgaactgtta
     7141 ttccataaga aagtatatcc cggtgttcct cctgaaacag tcacccaagc cttaccatta
     7201 ttaattccac atgtttcggg taatgagtaa atggctgagt taattgctga aggactattt
     7261 attgtcacgt ttattgagtc tctgcaattt gcagtatctc taaccactaa tttataggta
     7321 ccggccgcca gactaaaaat tgaatcagtg cctatttgtg tcggtgtcca tgtataagtt
     7381 aaaggtcctg agccacctgt aacatttact ttaatagcac ctgtgttttg tcctgaacat
     7441 tttacatgta tgatagtgtc aatagaaata accaaagaag gtgtattgct aacaataagt
     7501 gtgcgatttg cagtacaaag tttactgtcg gttacagtaa ccgtgtagct accaccgttt
     7561 aatcctgata ttgatgctgt actttgtcct gtattccaga gataggtata tggtgaagtg
     7621 ccgcctgtga catttactcc cgcactacca tttgcatttc cacaacgtgc cggtactata
     7681 gtgtcaatta atgttaatgc cgaaggctgt gttattattt gggttttatt tccaacacat
     7741 cctgttgaat cagtaacggt aacggtataa gttcctgcaa caatgttaac gatgtcctga
     7801 gtgattgcag cattactcca taaatagata acaggtccat tgtttcctga aacagaaata
     7861 taaactgcac cattagcctg accaaagcag cgagcatttt taacagagtc aacagtcacc
     7921 acagggccac tgatggttga aatattttgt gatgcagtct tagtacaaag agcagcatcg
     7981 gtaacagtca cagtataatt tcccggcaac agtcctgtaa ttgaagaagt agaagcacca
     8041 ttgctccaga gataagtata aggagaagtg ccaccggtaa cagcagagct aatccaacca
     8101 ttactttgcg cacaggttgc attgtgtgga gttagcgtaa ctgcaatgtc tgttggttgc
     8161 gaaacaactt gagttttatt tccaacacat cctgttgaat cagtaaccgt gacagtgtaa
     8221 gttcctgcaa caacgttaac gatgtcctga gtgattgcag cattactcca cagatatgta
     8281 acaggtccat tgttacctga aacggaaata taaacagcac cattagcctg accaaaacag
     8341 cgagcatttt taacagagtc aacagtcacc acaggaccac tgatagttgt aatattttgt
     8401 gatgctgtct tagtacacag tgcagcatcg gtaacagtca cagtataatt gccaggcaac
     8461 agtcctgtga ttgacgaagt agaagcaccg ttgctccaca gataggtgta aggcgaagtt
     8521 ccaccggtaa cagcagaact aatccaacca ttactttgag cacaggttgc attgtgtgga
     8581 gttaacgtaa caacaatgtt tgttggttgc gaaacaactt gagttttatt tccaacacat
     8641 cctgttgaat cagtaacagt aacggtataa gttcctgcaa caacattaac gatgtcctga
     8701 gtgattgcag cattactcca taaataggta acaggtccat tgtttcctga aacagaaata
     8761 taaacagcac cattagcctg accaaagcaa cggacatttt ttacagagtc aacagtcacc
     8821 acaggtccac tgattgtagt aatattttgt gatgcagttt tagtacacag tgcagcatca
     8881 gtaacagtaa cagtataatt tcccggcaac agtcctgtaa ttgaagaagt agaagcacca
     8941 ttactccaga gataggtgta aggcgaagtg ccaccggaaa cagcagaact tatccaacca
     9001 ttactttgcg cacaggttgc attgtgtgga gttagcgtaa cggcaatgtt tgttggttga
     9061 gtaatggctt gtgttttatt tccaacacat cctgttgaat cagtaacagt aacggtataa
     9121 gttcctgcaa caacgttaac gatgtcctga gtgattgcag cattgctcca cagatatgta
     9181 acagggccat tgtttcctga aacagaaata taaacagcac cattagcctg accaaagcaa
     9241 cgggcatttt ttacagagtc aacagtcacc acagggccac tgatggtagt aatattttgt
     9301 gaagctgttt tcgtacaaag tgcagcatca gtaacagtaa cagtataatt tcccggcaac
     9361 agtcctgtta ttgacgaagt agaagcacca ttgctccaga gataagtata aggcgaagtt
     9421 ccaccggtaa cagcagaact tatccaacca ttactttgcg cacatgttgc attgtgtgga
     9481 gttagcgtaa ctgcaatatc tgttggttgt gaaacaactt gtgtttttgt agctgtacaa
     9541 ccggaagaat cagtaacagt aactgtataa gtacctgcaa caacgttaac gatgtcctga
     9601 gtgattgcag cattgctcca cagatatgta acagggccat tgtttcctga gacagaaata
     9661 taaacagcac cattagcctg accgaagcaa cgggcatttt ttacagagtc aacagtcacc
     9721 acagggccac tgatggtagt aatattttgt gatgcagttt tagtacaaag cgcagcatcg
     9781 gtaacagtca cagtataatt tcccggcaac agtcctgtta ttgaagaagt agaagcacca
     9841 ttactccaga gataggtgta aggcgaagtt ccaccgataa cagcagagct aatccaacca
     9901 ttactttgtg cacaggttgc attgtgtgga gttagcgtaa cggcaatgtt tgttggttgc
     9961 gaaacaactt gtgttttatt tccaacacat cctgttgaat cagtaacggt aacagtataa
    10021 gttcctgcaa ctatgttaac aatgtcctga gtaattgcag cattactcca cagatatgta
    10081 acaggtccat tgttgcctga aatagaaata taaacagcac cattagcctg accaaagcaa
    10141 cgggcatttt ttacagaatc tacagtcacc acaggaccac tgatggttga aatattttgt
    10201 gatgcagttt tcgtacaaag tgcagcatcg gtaacagtca cagtataatt gccaggcaac
    10261 agtcctgtga ttgacgaagt agaagcaccg ttgctccaca gataggtgta aggcgaagtt
    10321 ccaccggtaa cagcagaact tatccatcca ttactttgcg cacaggtagc attgtgtggt
    10381 gttaatgtaa ctgcaatgtc aatgggctgt gtgatggctt gagtttttgt agctgtacaa
    10441 ccggaagaat cagtaacggt aactgtataa gttcctgcaa ctatgttaac gatgtcctga
    10501 gtaattgcag cattgctcca cagatatgta acgggtccat tgtttcctga aacagaaata
    10561 taaacagcac cattagcctg accaaagcag cgagcatttt ttacagagtc aacagttacc
    10621 acaggaccac tgatggtagt aatattttgt gatgcagttt tagtacagag cgcagcatca
    10681 gtaacagtta cagtataatt tcccggcaat agtcctgtta ttgacgaagt agaagcaccg
    10741 ttgctccaca gataggtgta aggcgaagtt ccaccggtaa cagcagaact tatccatcca
    10801 ttactttgcg cacaggtagc attgtgtggt gttaatgtaa ctgcaatgtc aatgggctgt
    10861 gtgatggctt gagttttagt agctgtacaa ccggaagaat cagtaacagt aacggtatag
    10921 gtgcctgcaa caatgttaac gatgtcctga gtgattgcag cattgctcca cagatatgta
    10981 acagggccat tgtttcctga gacagaaata taaacagcac cattagcctg accgaagcaa
    11041 cgggcatttt ttacagagtc aacagtcacc acagggccac tgatggttga aatattttgt
    11101 gaagctgttt tcgtacaaag tgcagcatcg gtaacagtaa cagtataatt tcccggtaac
    11161 agtcctgtta ttgacgaagt agaagcaccg ttgctccaca gataggtgta aggcgaagtg
    11221 ccaccggtaa cagcagaact aatccaacca ttactttgcg cacaggttgc attgtgtgga
    11281 gttagcgtaa ctgcaatgtc tgttggttcg gtgattgtat gtgaaagtga actctgacat
    11341 cctgtggaat cagtaattgt aactgtataa ttccccaaag gaatatttaa taaattctga
    11401 ctcactgaag cattgctcca caagtagtta ataggaccgt tggcactatt tacatggaca
    11461 taaattcttc ctgtactttg tccaaaacaa gttacattga aagcagagtc gagtactact
    11521 accggttgag tacttgcgcc aacaataaaa ctatcaacct ttgtgcataa attctgatca
    11581 gtaacggtta gtgtataaac acctgatgca agtatgttga gatcttcagt tgtgtttgaa
    11641 ttggaccata aatatccata aggagaaatg ccgccagaaa ttgaagcatc aatcattccg
    11701 ttaattaatc cacaagaagt atttgtgact gtagctgtaa cttttatttc atccggctca
    11761 tgtacagtat catttgcaaa aactgcagtg caattattca aatctgtaat tgttagagta
    11821 taaactcctg caatcagacc ggaaatatct tctgttccac ttccattaga ccattgaaaa
    11881 ctatagtttg ggacaccacc atcaacagaa atgtttatac ttccattatt caatccaaaa
    11941 catgttacgt cttgtattga aacagcgctt cgggtaattg catcgggttc tgtaagtgaa
    12001 tatgtttcaa ttgttgtatc gttggcaaca tcaaccacag ttactgtata tgaacctgct
    12061 gttattccac taatatcttc tgttgtagct ccattgctcc attgaatatt gaatgggccg
    12121 gaaccagtaa ttgttaaatc tatttggcca tcacttaaac cgttacaact gatgttgtaa
    12181 ccgttatagt ttgaaaatga agcagaaagt acaggctgtg caaaactctt tgttgtactc
    12241 aacaacaata acagggcgaa taagcttaaa taataaaatc tgaataagct ggttttcaga
    12301 tttttacaaa aagatttctg gcttatttcc ataaacaggt aaaagcggtg catgaatttt
    12361 aaagttctcg acccaaaaat agaaaaatcc gccaatatga tgatgtattt agtgaaaaaa
    12421 aatacattgt aataactgcc gatttttctt cgattttagt ttttaaatga atataacttt
    12481 aatgcacaat ttccgtacaa aaggaacaac atcatacaca atgaactctt taaagtccat
    12541 cattaccatt agtattttaa ctatactact gattttcgtt tcggctgatg gccagtcaca
    12601 accggttaat aatgcaagta caaagccccg tcaggttgat gtggtaattt gtatggacct
    12661 aagcggtagt accaacggac tactcgataa tttcagagat aaaatgtggg atatcatcaa
    12721 tcagtggcaa atgtttactc ccaaacctgt aattagaata gggttgattg gcttttccag
    12781 atattccttc catgatggca gcggatacat aaaaatcatt tctgatttaa cagatgatta
    12841 tgatgcgctt tcacaaaaaa tgttcgacat acaaccttat gtggagaatg gaattcagtt
    12901 tgtgggtgca gctataggtt caggagttaa tgatatgtca tggagtgaga agcctgatgc
    12961 tgttaaaatg atgtttgtaa ttgggtaatg gatatgttaa ccttggaagg tttgatttca
    13021 gagctcagtg tgagactgca ttaaaaagta atattgaaat tcatacagtt tattgtatta
    13081 aaaatgataa acaaatgcgt caacgtgagt tacccggttg ggaagagatt gctaaaatga
    13141 caggaggaca atcttttcag gtttttgtaa acaacagagc accattaatc aaggaaaaag
    13201 taaatgtgat ggcattagct tcattaaatg attcattgaa taaaactttt attccttatg
    13261 gtaaaaccgg tgttgaccgt ctgaaactta tgcttaaggc agatgaccat gctgtgcgat
    13321 cttatgaagc ttatttttat tcaaggttga agtacaaatt gagcgaccac tatacatcac
    13381 aaatgctcat gtgggatgta acacagtaca aagaagaata tccgcaatca cgccttgctg
    13441 cagtcagaca atctgtaaaa tctaataacg aatcatcagt tgatttggag ttacttgcaa
    13501 aagaatttgt agagaaaaga aatagcctgc gtgcatcaat caatgcaatg tttcctttgg
    13561 gtgaagagtt aagagtacat aacgaggctg ttgccgaaga gtatgaactt gacaacaggt
    13621 tagacaaaac aatggtgcga gcctattcac aaacacttct taaatttggg tttaaagaat
    13681 aagaattgaa gttgttctta ttaaaaacct gtttcataga ttagaaaatt gtgttttcta
    13741 tatctgattt aatcagctga aaatcgttga tgttatcttt gaaataccaa tctgttagct
    13801 tattttattt tcatgtttga aaacatttag ttctaaatgt tattc
//
LOCUS       DAOOOI010000056        13665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_898_length_13665_cov_7.389126, whole genome shotgun sequence.
ACCESSION   DAOOOI010000056 DAOOOI010000000
VERSION     DAOOOI010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13665
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_898_length_13665_cov_7.389126"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..4688
                     /gene="sprA"
                     /locus_tag="PKX59_07695"
     CDS             18..4688
                     /gene="sprA"
                     /locus_tag="PKX59_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOZ90787.1"
                     /translation="MAWYNIDPTAFYRSNSTVSLSAAERSNHNVRQISEKELFPKRQY
                     SNGIVPYMNTFDISFYPDERGPYNYETSARAGLSAGIDANGKLVDPTSRWGGFMRKVE
                     TSNFEESNIQYIQFWLMDPFNGDAPDSSMSNTGTLYFNLGDISEDVLRDGYESYENGL
                     PTTATPAAYRQNAWGRTPVAQNVLYAFDRDESTRKQQDVGLDGLGNDDEKLHFLTNFL
                     DYIDANVTNQTAKDAIHEDPSSDDFRYFRGDAYTNADGILQRYKNFNGTENNSPVAGS
                     GTFVPSSTQTPDAEDANRINGMEVDENYFQYKVDIDPSKLVVGQNYVTDVLENVAALN
                     LPDASGKTITWYLIKIPVNDPSRERIGQISDLKSVKFIRVFAKGFNGPKVLRLAKFEF
                     LRGEWRKYDYKLTGILSQPAVFDVGAVSLEENSNREPINYVLPPDFEREIDVTSTNQR
                     QLNEQSLSFKVCELADGDMKVCYKNTTLDMRNYKHVKMVIHAESFLPPANPTNDGELY
                     AVVRLGSDFTDNYYDYRIPLKMTPFGSTDPTVIWPKENDFDIDLDKLTDAKLERNNAG
                     VAFTDEYSKLDGNNVIFIKGNPTLSEVKVMMLGLWNPSGENPAFPPRPPDDGLAKCAE
                     VWFNEFRMSDFNDKGGWATIGRAQVKLADLGNVSVSGGYSTHGFGSLESKVGERSKED
                     VTNYDVATSLELGKFFPSSTGLTVPMYFGYGEVFSNPQYDPLDPDIEFSRSISNIDDS
                     KRKQNRKRFSQDYTRRKSLNFTNVKKNKTGKNASKSRIYDIENFNFTYGYTEVYRRNI
                     NTEYDIQKDYLGAIGYNFNTNPKPITPFSKMKAKSKYLRPIKDFNFNYMPSTFNFRWD
                     VNRHYGELKVRDVNAVLNQGVSDNSIPVLYDKRFTMNRQYGTSWDITRALKFEFNANN
                     QSKVDEPDGALNSEEKRDSVRKNFFNLGRNTDYMHNASLSYNLPLNKLPITDWISINT
                     RYSTTYHWTASPLIVNTDNGRYYTNPALGNTIQNSNSIQVNNSFNMTSLYNKWKLYKK
                     LTAPPKPKTSAKKEPKVDAKAPDAAKDSLKTKSVKPPEKKKEQEINPAIKALAKVIFS
                     LKNVSFNWNETNGLLLPGFNRQSEWLGQNWDGTSAPGFAFTFGSQNQDIRERAAKNGW
                     LTTDSTFNSQFTSTYTQNITGRASLEPFTGFNIDVNFNRNYSLSTTENYRAYNSPRAN
                     GRYYETLARVETGNFSISNLMWGTSFTKDRKDYSSTIFERFAENRRILSERLGNENPN
                     SSGVGADGYRDGYSGTSQEVVILSFLSAYSKKSASRMSLDLFPSVPLPNWTVRYDGLT
                     KIAFVKNLFQSVNLNHGYRSTYNVNSFTTDVSYVPGGNARDINMDFIPKRQIGQVSLS
                     EQFSPLIGIDITWKNRNDKSSGSKSATERGGRSSGGNFTTRFEYKRDRNISLTLAGIQ
                     VTEVKGREFTFGAGYRIPNFKFPFGLFKSVNSRRSNDLNTTLDFSVRKNTTILRKLVE
                     GTNQPTAGLTIISIKLASDYTVSERLNLRLFFEKTINTPVVSTSYPNANTAFGITIRF
                     TLSQ"
     gene            complement(4722..5846)
                     /gene="carA"
                     /locus_tag="PKX59_07700"
     CDS             complement(4722..5846)
                     /gene="carA"
                     /locus_tag="PKX59_07700"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOZ90788.1"
                     /translation="MKYAHKSPAVLLLQDGTVFYGKSAGKIGTATGEICFNTGMTGYQ
                     EIFTDPSYKSQIMVMTSVHIGNYGVCQAESESSSAKISALVCRNFSDHFSRKKADQSL
                     QDYFIENNMVVVSDVDTRAIVQHIRSKGAMNAIVSSEETDLAKLKTLLAKVPSMEGLE
                     LSSTVSTQKAYTYGDAAAALRVAVIDLGVKENTLRCLADRGCYIKVFPMNTSFEEMEK
                     FNPQGYLVSNGPGDPAVMPAMVDTVKKILNSDKALMGICLGHQMLAEACGIKTFKMFN
                     GHRGINHPVKNLVTGRAEVTSQNHGFGVIPEETINKAEIEITHINLNDQSIEGISVKN
                     KKAFSVQYHPEAAPGPNDARYLFDNFVNAVREVKEQAVTV"
     gene            complement(5919..6608)
                     /gene="radC"
                     /locus_tag="PKX59_07705"
     CDS             complement(5919..6608)
                     /gene="radC"
                     /locus_tag="PKX59_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOZ90789.1"
                     /translation="MEEPKNIKSWSESDRPREKLLEKGKEALTDSELLAILIRSGTRQ
                     ESAVDLAKRILKNTHNELSELSKLSVKDMMKYKGMGKVKSITIVAALELGRRRAQAQA
                     LERQKITSSKDAVAYFKALLADLPHEEFRILLLDRANQIIKSENISKGGVSGTVVDAR
                     IIFKSAIENLASGIILCHNHPSGNPKPSQADVNLTSKLSQAGKLLEINILDHVIIAGD
                     DYYSFADEGQL"
     gene            complement(6682..6936)
                     /gene="rpsT"
                     /locus_tag="PKX59_07710"
     CDS             complement(6682..6936)
                     /gene="rpsT"
                     /locus_tag="PKX59_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOZ90790.1"
                     /translation="MANHKSAVKRIRANGAKKDRNRYQAKTSRNALKELRTTTDKKEA
                     EKLLPEVSGMLDKMAKKNLIHKNKAANLKSSITKYVSALK"
     gene            complement(6987..7058)
                     /locus_tag="PKX59_07715"
     tRNA            complement(6987..7058)
                     /locus_tag="PKX59_07715"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7023..7025),aa:Glu,seq:ttc)
     gene            complement(7282..8403)
                     /locus_tag="PKX59_07720"
     CDS             complement(7282..8403)
                     /locus_tag="PKX59_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ90791.1"
                     /translation="MAEYRFNRELISLGRLNVTSGVYFQQSFSHASLYAGSGSDKNTL
                     ENYAAFIQLEKKLIKKLMVSAGFRTEYFEMNNKESQLEPILRAGLNYQLTEGTFIRCS
                     YGQGYRYPTIAEKYIETGAGGISVFPNPEVQPESSWNAEAGIKQGFKIGNFFGFADFA
                     VFEQRYHNTIEYIYALWQPDQLAGFKFVNTGNSRVKGLEVSIMGEGKPVHNFAISFLG
                     GYTYTLPQSLEPDYIFATDNPPTDFLPDSLSYSSTSTDTSKNILKYRFQHIAKMDIEL
                     TWKFISLGYGIRYYSFMKNIDKKFYDLDETVLPTGVKAYREKHDKGTLVHDIRLGFKL
                     NEIFKASFLVNNATNLEYSLRPLKIESPRNYTVQLTAAF"
     gene            complement(8441..9679)
                     /locus_tag="PKX59_07725"
     CDS             complement(8441..9679)
                     /locus_tag="PKX59_07725"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOZ90792.1"
                     /translation="MRYLNNAKKSFLLIIANVLFGISCYAQMGVLRGNVYLKQNGEPI
                     AGAGIFPVNDKSIGVASDADGSYEINLPVGKQTIVYTLTGLKNDTLTLDIKDGTESIR
                     NIYLTSNLKNLNVVVVSASKYEQRIEDITVSMEVISPKLIENRNTTNVTSVLEQTPGV
                     TILDQEPQIRGGSGFSFGVGSRVATLIDGLPIMTGDAGRTEWQFIPVENIEQIEVIKG
                     ASSVLYGSSALSGTINFRTAYPKERYHTYLRTYGGVYDKPSNRDAKWWDGPATFSGIN
                     FLHSEKINRWDLIIGGNGLYDHGFIGPPLAMSSLPFQDTTISNNDVASRWGRLNFNIR
                     HRFKNVEGLSAGINGNFMQSHSNFSLVWANDSTGIYNAFPKTMTLTDSKTFYIDPYIS
                     LTGKKRFITSTAFTHFLCRQ"
     gene            complement(9681..>10447)
                     /locus_tag="PKX59_07730"
     CDS             complement(9681..>10447)
                     /locus_tag="PKX59_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90793.1"
                     /translation="ADWNNLNPSTGIIGVLTCARASGADAHSGSYAIKLTTKLIPGFN
                     VIANGIASTGTLITTPPYGVSGGIAFTGRPDSITGWYKYTPAGTDQGFVELQLLGTTN
                     TDTIGYVRFETPNATVSTYTRFAAAVNYLSTATPSNTIWILSSSKGVGPIVNSQIFID
                     DIAMVFNPSSVNTIDTKNEVSILGNVIKEKIRFNYKNTGVYQFKLIDTAGRIVTDTEL
                     NSQQSTVDVSALDGLYFYIIENNSGIINNGKVIIRN"
     assembly_gap    10448..10457
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10865..11290)
                     /locus_tag="PKX59_07735"
     CDS             complement(10865..11290)
                     /locus_tag="PKX59_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90794.1"
                     /translation="MAKRYSVFQWLFSFITPVIIHKDQSEVNSILETTLENGKLVLNS
                     AHANYSYGSLFRVFIKALLRFNIDIKNKKKILVLGMGGGSVVKFLLGNNTYAQLDTVE
                     IDRKIISIAKEMFDLSPSDRLKIYAANAVDYLKDCTEEY"
     gene            complement(11396..11989)
                     /locus_tag="PKX59_07740"
     CDS             complement(11396..11989)
                     /locus_tag="PKX59_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOZ90795.1"
                     /translation="MKLISLLCISLLMLVSCKENSEPKQGAETTEQLPSNSIFNLTSD
                     WQNQDGKTIKLKDLQGKTLVVVMIYTTCKTACPILVAKMKMIESKFDRKDIDKINLVL
                     VSIDPETDTPEHLKEFSIKNEMTGPQWTFLRSAENATKEFANVLSMKYKKISPIDFSH
                     SNIISTFKPNGILVSQEEGDINVDKVVTTVNQTVRKK"
     gene            complement(12002..12844)
                     /locus_tag="PKX59_07745"
     CDS             complement(12002..12844)
                     /locus_tag="PKX59_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOZ90796.1"
                     /translation="MKHLIILLFSIVIVSCSKESEKETVYKNLEGNKTDTLQSIQSTV
                     KMVYIPGGEYKPFFGSNTTLVSVAPFLMDERAVTNEEFLEFVIANPQWQKSNIKRLFA
                     DTNYLKTWPTDITLPENALKDAPVCNVSWFAAKAFAQSVDKRLPTLDEWEFVAMADET
                     TANARKKPKYSDSIIDLYLQKNRQFKSVKQSPPNYWGIYNMFDLIWEWTDDFNAVLTT
                     GDSRSGEYNDKNLFCAGGATSATDVMNYAAYMRFALRTSIKADYTIGNLGFRCAKDTT
                     IIKQ"
     gene            complement(12874..>13665)
                     /gene="nirK"
                     /locus_tag="PKX59_07750"
     CDS             complement(12874..>13665)
                     /gene="nirK"
                     /locus_tag="PKX59_07750"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HOZ90797.1"
                     /translation="FWTFNNTVPGSFIRVREGDEVTLNLKNDGSSILPHNIDLHAVTG
                     PGGGAAATTAPPGKEASFTFKAINKGLYVYHCAAPPVPMHIGNGMYGLILVEPAGGLK
                     PVDREYYIMQGDFYTKASVVKQGLMEFDNDKAVAENPDYVLFNGKKGSLLGANMLEAK
                     VGETVRLFVGNGGPNLTSSFHVIGEIFDNVYLEGGSTVSHNVQTTMIPAGGSAIVEFK
                     CEVPGEYVLVDHSLSRAFNKGCIGKLKVTGEPNEKVFSAKTLTKE"
BASE COUNT         4174 a         2563 c         2590 g         4328 t
ORIGIN      
        1 gctaaaatcg tgctaaaatg gcttggtaca atattgaccc gactgctttt tacagaagca
       61 acagtacggt gtcattatct gctgcagaaa gaagtaatca taatgtaaga caaattagtg
      121 aaaaggaatt attccccaag cgacaataca gcaatggtat tgttccttac atgaatacat
      181 tcgatatttc tttttatcct gatgagcgtg gaccttacaa ttatgaaaca tctgcacgtg
      241 caggtttatc tgcaggtatt gatgctaacg gaaagttggt tgacccaaca tcacgttggg
      301 gtggttttat gcgaaaagta gaaacgagca actttgaaga gtcaaacatt cagtacatac
      361 agttttggtt gatggatcct tttaatggcg atgcacctga ttccagtatg tcaaatacag
      421 gaacgcttta tttcaacttg ggtgatatct ctgaagatgt gttgcgtgat ggatatgaaa
      481 gttatgagaa tggtcttcca acaacagcaa caccggcagc atatcgtcag aatgcatggg
      541 gccgcacacc tgttgcacag aatgtacttt acgcttttga cagagatgaa agtacacgta
      601 aacaacagga tgttggttta gatggtttgg gtaatgatga tgagaaacta catttcctca
      661 caaacttcct cgattacatt gacgccaacg taacgaatca aactgcaaag gatgccattc
      721 atgaagaccc ttcgtcagat gatttcagat atttcagagg tgatgcttat accaatgccg
      781 atggaatatt gcagcgttat aaaaacttca acgggacaga aaacaattca ccggttgcag
      841 gcagtggaac atttgtgcca tcaagtacac aaactccaga tgcagaagat gcaaaccgta
      901 ttaacggtat ggaagttgac gaaaattatt ttcaatataa agttgatata gatccttcaa
      961 aattggtagt aggacaaaac tatgtaacag atgtgctgga gaatgtagct gcattaaatc
     1021 ttcctgatgc atcaggtaag accatcacat ggtatctgat aaaaattccg gtgaacgatc
     1081 caagtcgtga gcgcattggt caaatctcag atctgaagtc cgtaaaattt atccgtgttt
     1141 ttgcaaaagg atttaacgga cccaaggtgc tgcgccttgc aaaatttgaa ttcctgagag
     1201 gtgaatggag gaagtatgat tataagctta caggtattct tagtcagcct gctgtttttg
     1261 atgtaggtgc tgtttcactc gaagagaaca gcaaccgcga gccgattaat tatgttttgc
     1321 ctccggactt tgagcgtgaa attgatgtta cctcaaccaa tcaacgccaa cttaacgagc
     1381 agtcgctttc gtttaaagtg tgcgaacttg ctgatggtga tatgaaggtg tgttataaaa
     1441 acacaacact cgacatgcgt aattacaaac atgtaaaaat ggttatccat gccgaatcgt
     1501 ttctaccacc tgccaatcca accaacgatg gtgagcttta cgcagttgtt cgtttaggct
     1561 ctgactttac agataactac tacgactacc gcattccatt aaaaatgact ccttttggaa
     1621 gtacagatcc tacagtaatt tggcctaaag agaatgattt tgatattgat ttggataaac
     1681 tgaccgatgc taagctggag cgtaataatg caggtgttgc atttacagat gaatattcta
     1741 agcttgatgg caacaatgta attttcatta aaggtaatcc tacattgagc gaagtgaaag
     1801 ttatgatgct tggattgtgg aacccaagtg gtgagaatcc tgcattcccg ccacgaccac
     1861 cggatgacgg attggcgaag tgtgcagaag tatggttcaa cgaattccgc atgtccgact
     1921 ttaatgataa aggcggatgg gcaactatag gtcgtgcgca ggtgaagctt gccgatttgg
     1981 gtaatgtttc tgtttccggt ggctattcaa ctcatggatt cggaagttta gaaagtaaag
     2041 ttggtgagcg tagcaaagaa gatgtgacaa actatgatgt agccacatca cttgagttgg
     2101 gtaagttttt tccatcaagt acaggtctta cagtgccaat gtattttggt tatggtgaag
     2161 tattcagcaa tccgcaatat gatcctcttg atcctgatat tgaattcagc cgttcaatca
     2221 gcaacattga cgattcaaaa cgcaaacaaa acagaaaacg tttttcgcag gattatactc
     2281 gaagaaagag tttgaacttt accaatgtca agaaaaataa gacagggaag aatgcatcta
     2341 agtcaagaat ttatgacata gagaacttta attttactta tggatatact gaagtttatc
     2401 ggagaaatat caatacggaa tatgatattc agaaagacta ccttggtgca ataggttata
     2461 attttaatac aaacccaaaa ccgattacgc ctttcagtaa aatgaaggct aagtcgaagt
     2521 acctacgtcc tattaaagat tttaatttca actacatgcc atcaaccttt aatttccgat
     2581 gggatgtaaa cagacactat ggcgaactta aagtgcgtga tgtaaatgct gtattgaatc
     2641 agggtgtttc tgataattct attcctgtgt tgtatgataa gcgttttaca atgaaccgtc
     2701 agtatggaac aagttgggac atcacccgcg cattgaagtt tgagtttaat gccaacaatc
     2761 aaagcaaggt tgacgagccc gatggtgctt taaactcaga agaaaaacgc gattctgtaa
     2821 gaaaaaactt tttcaactta ggacgtaata cagattacat gcacaatgca tctttatcgt
     2881 ataatcttcc attaaataag ttgccgataa cagattggat aagcattaat acgcgctaca
     2941 gcactactta tcactggaca gcatcacctt taatagtaaa cacagacaat ggaaggtact
     3001 atacaaaccc tgccttaggc aacactattc aaaactcaaa ttctattcag gttaacaaca
     3061 gctttaacat gacttcttta tacaataagt ggaaactgta taaaaagttg acagcaccac
     3121 caaagccaaa aacatctgct aagaaagagc caaaggttga tgctaaagca ccggatgctg
     3181 cgaaagacag tttaaagaca aagagtgtta aaccaccgga gaagaaaaaa gaacaggaaa
     3241 taaatcctgc tataaaagca ttggcaaaag ttattttcag tttaaagaat gtttcgttta
     3301 actggaatga aaccaatgga ttgttgttgc ccggctttaa cagacagagt gaatggttag
     3361 gtcagaattg ggatggcacc agtgcaccgg gatttgcatt tacttttggt agtcagaatc
     3421 aggacatcag agaacgtgcg gccaagaatg gatggcttac aacagactca actttcaaca
     3481 gtcagtttac ttctacctac acacaaaaca tcacaggaag agcttctctt gagcctttta
     3541 caggatttaa tattgatgta aattttaacc gcaattactc tttaagtacg acagaaaatt
     3601 acagagccta caacagtccg cgtgctaacg gtcgttatta tgaaacattg gcaagagtag
     3661 aaaccggtaa tttcagtatc agcaatctga tgtggggaac atcattcact aaagacagaa
     3721 aagattattc atcaaccatc tttgagcggt ttgcagaaaa cagaagaata ctttctgaaa
     3781 gattgggcaa tgaaaatcca aactcatcag gtgttggcgc tgatggctac agagatggct
     3841 acagtggaac atcacaagag gtagtgatac tttcattcct ttcagcttat tctaagaaga
     3901 gtgcctcacg catgtcgctc gatttgtttc catcggtacc actacctaac tggacagtgc
     3961 gctatgacgg acttaccaaa attgcatttg taaaaaatct attccagagt gtaaacctta
     4021 accatggtta tcgttctact tacaatgtaa acagctttac tactgatgtt tcttatgttc
     4081 ccggtggtaa tgcgcgcgac atcaacatgg attttattcc taagagacag atagggcagg
     4141 tgagtttgag tgaacagttt agtcctttaa tcggaataga catcacctgg aaaaacagaa
     4201 atgataaatc atcaggaagt aagtcggcaa cagagcgtgg cggacgttct tccggtggaa
     4261 atttcaccac acgtttcgag tacaagcgtg atagaaatat atcattaaca cttgcaggta
     4321 ttcaggtaac agaagttaaa ggacgtgagt ttacatttgg agcaggctat cgaattccaa
     4381 actttaagtt tcctttcgga ctgtttaaga gtgttaactc acgcagatca aacgatctga
     4441 atacaacatt agatttctca gttcgtaaga acactaccat tctcagaaaa ttggttgaag
     4501 gtaccaatca gccaacagca ggtttaacca tcatctctat taagcttgct tctgattata
     4561 cggtaagtga acgacttaat ttaagattgt tcttcgagaa gactatcaat acacctgtgg
     4621 tgtcaacatc ttaccccaat gcgaatactg catttggtat tacaataaga tttacactgt
     4681 cgcagtagga gtcctaaaat aaaataattg aagagggcag gctacaccgt aacagcctgc
     4741 tcttttactt ccctaacggc atttacgaag ttgtcgaaaa gataacgcgc atcattaggg
     4801 ccgggtgcag cttcgggatg atactgaaca gaaaaagcct ttttattttt tacactgatt
     4861 ccttctatac tttgatcgtt caggtttata tgtgtaattt caatctctgc cttattgata
     4921 gtttcttccg gaataacacc gaagccatga ttttgtgatg tcacttctgc acgacctgtc
     4981 accaaatttt ttactgggtg attgatgcca cgatgtccgt taaacatttt aaatgttttt
     5041 attccacagg cttctgcaag catctgatga cctaaacaga ttcccatcag cgctttatcg
     5101 gaattcagaa tttttttcac ggtatctacc atagcaggca ttacagcagg atcgcccggt
     5161 ccattggaaa ccaaataacc ttgcggatta aatttttcca tttcttcaaa agaagtgttc
     5221 atcggaaaaa cttttatgta acaacctctg tcagcaagac agcgcaatgt gttttctttt
     5281 actcctaaat ctattacagc cacacgcaat gctgctgctg catcgccata ggtatatgct
     5341 ttctgagtgc tgactgtgct cgataattca agtccttcca tagaaggtac ttttgccaaa
     5401 agggttttca gctttgcaag atcggtttct tcagaagaaa caatggcatt catagcacct
     5461 ttgctgcgga tgtgctgcac gatggcacgt gtatcaacat cagatacaac aaccatattg
     5521 ttttcaataa aataatcctg aaggctttga tcggcttttt tgcgtgaaaa atggtcagag
     5581 aaattgcgac aaaccaatgc agatattttt gctgacgaag attctgactc tgcctggcac
     5641 acaccataat ttccgatgtg tacagaggtc atcaccataa tctgcgattt ataacttggg
     5701 tcggtaaata tttcctgata gcctgtcatg ccggtgttaa aacagatttc accggtggca
     5761 gttccaattt tacctgccga ctttccataa aataccgtac cgtcctgaag caacaataca
     5821 gcgggtgatt tgtgtgcgta tttcatttcg aaaagtttcg caaaagtacc attaaatcgg
     5881 ttatcagatt ttttacttgt aaacaggctg ttgaaaaatc aaagctgccc ttcatcggca
     5941 aagctgtaat agtcgtcacc ggcaataata acatgatcta agatgtttat ttccagtaat
     6001 tttccggcct gacttagttt tgatgtcaga ttaacatcag cctgacttgg tttaggattg
     6061 ccactagggt ggttgtgaca aaggataatg cccgaagcta aattttctat agccgattta
     6121 aaaatgattc gtgcatcaac cacagttccc gaaacaccac ctttactgat gttttcggat
     6181 ttgataatct gattggcgcg gtctaacaaa agtattctga attcttcatg cggcaggtcg
     6241 gccagtaagg ctttaaaata ggctactgca tctttgcttg aagttatttt ttgtctttcc
     6301 aaagcctgtg cttgtgcgcg tctgcgtcct aattccaaag cagcaacaat agtgatagac
     6361 tttactttgc ccataccttt atacttcatc atgtctttta cggatagctt agaaagttct
     6421 gacagctcgt tatgtgtatt ttttaaaata cgtttggcca agtcaacagc agattcctga
     6481 cgggttcctg aacggatgag gatagctagc aactcactgt cggttaaggc ttccttgcct
     6541 ttttcaagga gtttctctct tggcctgtca ctttccgacc atgatttgat gtttttaggt
     6601 tcttccatgg tgctaagata aggtgttaaa caaaaaaagc ccgcatttat agcgagctta
     6661 aaattcaaaa ttatgtgaat attattttaa agcactaaca tatttagtga tacttgattt
     6721 cagatttgca gccttatttt tatgaataag gttctttttt gccattttat caagcatacc
     6781 tgatacttcc ggaagcaact tttcagcttc tttcttatct gtggtagtac gcagttcttt
     6841 caaagcatta cgcgaagttt ttgcctgata gcggtttctg tcttttttag caccgttggc
     6901 gcgaattctt tttactgctg acttgtgatt tgccataatt acagttttta ctttttaatg
     6961 atggatatta atccttattt tttttgtagc ccgtacgaga atcgaactcg tgtttcctcc
     7021 gtgaaaggga gatgtcctaa cccctagacg aacgggccaa acgttttttt gaggctgcaa
     7081 atgtataaaa acaatttaca ttcaaagcct tatagtgtaa atatttttca tttttctgct
     7141 atttggctga agtattctga cttctgtata gaaaatcatc attaacaacc taagtaagac
     7201 tgaattaaag ctattgaaca ggtcagattt aacagcccaa aataaactca taaacacctt
     7261 tttaacaagc taaggtctat attaaaatgc tgccgtaagc tgaacagtat aatttctggg
     7321 cgactcaatt tttaacggac gcaatgaata ctcaaggttt gtggcattgt ttactaaaaa
     7381 tgaagcttta aaaatttcgt tgagtttgaa gcctaaacga atatcgtgca ccaaggttcc
     7441 tttgtcatgt ttttcgcgat aggcttttac accggtcggt aaaactgttt catcaagatc
     7501 ataaaacttt ttatcaatat ttttcatgaa actgtaataa cgaatgccat aacctaaaga
     7561 aataaatttc caggttagct ctatatccat tttggcaata tgctgaaaac ggtatttgag
     7621 aatattcttt gatgtgtctg tgctggtgct gctgtatgat aatgagtcgg gtaaaaagtc
     7681 tgttggcgga ttatcagttg caaaaatata gtcaggctcc agactttgcg gcagcgtata
     7741 agtatatcca cccagaaagc ttatggcgaa attgtgaacc ggttttcctt cacccataat
     7801 agaaacttca aggcctttaa cacgtgagtt tcctgtattc acaaatttaa atccggctaa
     7861 ctgatcgggt tgccataaag cataaatata ttcaatggtg ttgtgatagc gttgttcaaa
     7921 aactgcaaaa tcggcaaagc caaagaagtt tccaatctta aaaccttgct taattcctgc
     7981 ttcggcattc cagcttgatt caggttgtac ctctggattt gggaaaacag aaattccacc
     8041 ggcaccggtt tcaatatatt tttctgcaat ggttggataa cgatatcctt gtccgtatga
     8101 gcaacgaata aaagttcctt ctgtaagctg ataatttaat cctgcacgta aaataggttc
     8161 taactgcgat tctttattgt tcatctcaaa gtattcagtt ctgaaacctg cagacaccat
     8221 caattttttt ataagttttt tttctaattg tataaaagct gcatagtttt caagggtgtt
     8281 tttatcgctt ccactacctg cataaagtga tgcatgactg aatgattgct ggaaataaac
     8341 tccagaggtt acatttaatc ttcctaatga aatcaactcg cggttaaaac gatattcagc
     8401 cagataaaca tttgttttat ttgattgatc gttgccattg tcattgtcgg cataaaaaat
     8461 gcgtgaacgc agttgatgtg atgaaccttt tttttccggt cagactgatg tatgggtcaa
     8521 tataaaatgt tttagaatcg gtaagtgtca ttgttttcgg gaaggcattg taaattcctg
     8581 tgctgtcatt tgcccaaacc aatgaaaagt tagagtgtga ctgcataaaa tttccattga
     8641 tacccgctga aaggccttcg acatttttaa acctgtgacg gatgttgaaa tttaatctgc
     8701 cccagcgtga tgcaacatca ttgttactga tggttgtatc ctgaaaaggt aaagaactca
     8761 tagctaaagg cgggccaata aatccgtggt catacaatcc attgccacca atgattaaat
     8821 cccaacgatt gattttctca ctatgtaaaa agttgatgcc tgaaaaagtt gcagggccat
     8881 cccaccattt tgcatctctg tttgatggtt tgtcataaac gccaccataa gttcttaaat
     8941 aggtgtgata acgctctttt ggataggctg tcctgaaatt gattgttcca cttaatgcag
     9001 acgaaccata taaaactgaa gaagcacctt taattacttc tatctgctca atattttcga
     9061 caggaataaa ttgccattct gtacgtcctg catcaccggt cataatggga aggccgtcaa
     9121 tgagtgtagc tactcgactg ccaacaccaa aactaaaacc actaccaccg cgaatttgtg
     9181 gctcctgatc taaaattgta acacccggag tttgttcgag tacactagta acattggttg
     9241 tgtttctgtt ttcaataagt tttggactga taacttccat gcttactgta atatcttcaa
     9301 tacgttgttc gtacttactt gcagaaacaa ctactacatt cagattttta aggtttgatg
     9361 taagatagat attgcggata gactctgtac catctttaat atctaatgta agggtatcat
     9421 tttttaatcc tgtcaatgta taaacgatgg tttgttttcc aacagggaga tttatctcat
     9481 aacttccatc agcatcagat gccacaccta ttgatttatc attgaccgga aaaataccgg
     9541 cacctgcaat aggttcacca ttttgtttta agtaaacatt tcctctcaac acacccattt
     9601 gtgcatagca ggaaatgcca aacaagacat ttgcaataat taatagaaat gatttttttg
     9661 cgttattcag atagcgcata ttaattcctg ataattactt ttccgttatt gataatgcct
     9721 gaattatttt cgatgatata aaaatacaaa ccatccaaag cagaaacatc tacagtactt
     9781 tgttgtgagt taagttctgt atctgttact attcgtccgg cagtatcaat taatttgaat
     9841 tgataaacac cggtattttt ataattaaat cgtatttttt cttttatgac attgcccaga
     9901 atggaaactt cgttttttgt atcaattgta tttacagaag aagggttaaa aaccattgca
     9961 atatcatcaa taaaaatctg actgttgaca ataggtccga ctcctttgct tgaggaaagt
    10021 atccatatag tgtttgatgg tgttgctgta gaaagataat ttacagcagc agcaaatcgt
    10081 gtgtatgtac ttactgtggc attaggtgtt tcaaatctta catagccaat agtgtctgtg
    10141 tttgtagtgc ctaacaattg caattcaaca aatccctgat ctgtaccggc aggtgtatat
    10201 ttataccaac ctgtaatact atccgggcga ccggtaaagg caatgcctcc tgatacgcca
    10261 taaggtggtg ttgtgattaa tgtgcctgta cttgctatac catttgctat aacattaaat
    10321 ccgggaatta atttagtagt taatttaatt gcatagcttc cactgtgtgc atctgcacct
    10381 gaagcacgag cacaagttaa cacacctatt attccggtgc ttggattcag attattccaa
    10441 tcggcagnnn nnnnnnnacc tgattccagt tttcaaaacc tgcattaggc gttgcatttt
    10501 gtgcctgaag gctaatggct actaaagtta aaaatatagt tgtgtaaagt cttttcatta
    10561 gtaatggagt ttgcattaac cggacaaata tagtgcaatg aattaaaatt ttatagtgat
    10621 agtaaattac ttactcacag caggttgtaa caggaaaaac agattttgtt ctatggtaag
    10681 attctctttt aagtgtattg cgcctaaaaa atcaagattg tgttgaaggt tattttgctt
    10741 catactggca ttaaaaacaa tgtcacattt aggcagacaa atgttttgaa ttgatttcca
    10801 aaaagattct gtgaaacaaa actcaggaac agcatcgtca ataaaaaggt ctatcaaaat
    10861 taaatcaata ttcctctgta caatctttaa ggtagtcaac ggcattagct gcataaattt
    10921 ttaatctatc agaaggagaa aggtcaaaca tttcttttgc aatgctaatt atcttccggt
    10981 caatttcaac agtatcaagt tgtgcatatg tattgtttcc taataaaaac tttacaacac
    11041 tgccaccgcc cataccaaga actaatattt tctttttgtt tttgatgtca atattaaaac
    11101 gaagcaatgc ttttataaaa acacgaaata gacttccata gctgtaattg gcatgagcag
    11161 aattcagaac cagtttgcca ttttccaatg tagtttctaa aatactattt acttccgatt
    11221 gatctttgtg aatgataaca ggagttataa aactaaacaa ccattgaaat acagaatatc
    11281 gttttgccat tatgcaaaga taactacgta gaatattcta tggaagcaaa gggagttaac
    11341 tactgcctaa aaagtagttt tatgttaaat gagattgttc tcttttttaa ttgttttatt
    11401 tctttcttac agtttgattg actgttgtaa caaccttgtc aacattaata tcaccttctt
    11461 cctgacttac taaaatccca tttggtttaa aggtgctgat aatgtttgag tgtgaaaaat
    11521 caataggtga aatctttttg tatttcattg aaagtacgtt tgcaaattct tttgttgcgt
    11581 tttcagcaga gcgtaagaat gtccattgtg gccctgtcat ttcgttttta atcgaaaact
    11641 ctttgagatg ttccggagtg tctgtttcag ggtcaataga tactagtaca agattgattt
    11701 tatcaatgtc tttccgatca aatttagatt caatcatttt cattttagca acaagtatag
    11761 ggcatgcagt tttacaggta gtgtaaatca taactactac cagtgtctta ccctgtaagt
    11821 cttttagctt aatagttttc ccatcttgat tttgccagtc ggaagtaaga ttgaaaattg
    11881 agttggatgg aagctgctca gtagtttctg caccttgctt aggttcagaa ttttctttgc
    11941 atgaaacaag cattaatagt gaaatgcata atagtgaaat gagtttcata aaattatagt
    12001 gttactgttt aataattgtt gtgtcttttg cacaacggaa tcctaaattt ccaatagtgt
    12061 aatcggcttt gatggatgta cgcagtgcaa atctcatata tgctgcataa ttcataacat
    12121 cggttgcaga tgttgcgcca cctgcacaga aaagattttt gtcattatat tctccacttc
    12181 gcgaatcgcc tgttgttagt acagcattaa aatcatctgt ccattcccaa atcagatcaa
    12241 acatattata aataccccaa tagtttggag gactttgttt aaccgatttg aattgtctgt
    12301 ttttttgaag gtataaatca atgatgctat ctgaatattt tggttttttt cttgcattgg
    12361 ctgttgtttc atctgccata gctacaaatt cccattcatc taatgttggc aaacgtttat
    12421 caacactttg agcaaatgct ttggcagcaa accatgatac gttacatacc ggagcatctt
    12481 tcaatgcatt ttcaggtaat gtaatgtctg taggccatgt ttttaaatag ttggtgtctg
    12541 caaacagtct tttaatattt gatttttgcc attgtggatt tgcaatgaca aattcaagaa
    12601 attcttcgtt ggtcacagct cgctcatcca ttagaaaagg agctacactc accaatgtag
    12661 tgttagaacc aaagaatggt ttatattctc ctccgggaat ataaaccatc ttaacggtac
    12721 tttgtatgct ttgtaatgta tcagttttat tgccctctaa gtttttgtaa acagtttcct
    12781 tttcggattc cttagagcaa gatacaatta caatagaaaa taaaagtatg attaagtgtt
    12841 tcacgttgca ttttattgct tacaaaaggc gggttactct tttgtcagag tttttgcact
    12901 gaatactttc tcattaggct caccggtaac tttaagtttt ccaatacagc ctttgttaaa
    12961 tgctcttgaa agtgagtgat caaccaatac atattcgccc ggtacttcac atttaaattc
    13021 aacgatagct gatcctcctg caggaatcat tgttgtttga acattatgtg aaacagtaga
    13081 tccaccctct agataaacat tgtcaaaaat ttctccaatt acgtgaaatg aagatgttaa
    13141 attagggcca ccattgccaa cgaaaagtct tacagtttct ccaactttag cttcaagcat
    13201 attagctcca agtaatgaac ctttttttcc gttaaataaa acataatccg gattttcagc
    13261 aacagctttg tcattgtcaa attccattaa accttgttta actactgatg cttttgtata
    13321 gaaatcacct tgcataatgt agtattctct gtcaacaggc tttaatccac cggcaggttc
    13381 aaccaatatc agaccataca ttccattacc tatatgcata ggaacaggag gtgcagcgca
    13441 atggtaaaca taaaggcctt tatttattgc tttaaaagta aacgaagctt ctttgccggg
    13501 aggcgctgtg gttgctgcag caccgccacc gggtccggtt acagcatgca ggtcaatgtt
    13561 atgcggcaat atactgctgc catcattttt aagatttaat gtcacttcat cgccttctct
    13621 tactcggata aaacttcccg gtacggtgtt gttaaacgtc cagaa
//
LOCUS       DAOOOI010000057        12443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_994_length_12443_cov_16.815224, whole genome shotgun sequence.
ACCESSION   DAOOOI010000057 DAOOOI010000000
VERSION     DAOOOI010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12443
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_994_length_12443_cov_16.815224"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..2063
                     /locus_tag="PKX59_07755"
     CDS             27..2063
                     /locus_tag="PKX59_07755"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HOZ90798.1"
                     /translation="MTTKIAKTKRLFHLSRITFFLLCLFITGLAKAETEPNDSKAQAN
                     TLTLNTGMPGTVALSESDWFSVTTPEDGNLNITGTFSSYIYIQVYDNDGTTNLFLNYH
                     SGTVTRTVYGLAAGTYYVRLYNYTATETNTYTITPSLTATGFANDTEDNSTPALANVL
                     PLNGSVTGHVGYYYNNQRDTIDWYAVTVTQDGTLSYTITSENGLYIYAKLYDGDGTTQ
                     LAGNYTGSTATFSRGDMAPGVYYILIHAYYPNSEYVPYTLSNTLIPPVSANDPLPNET
                     FSTATPLALNGSTTGHIGYRYNGNVDVYDWYSVNTNADGDLYFTITSDNGQYVYAELF
                     DGDGITNLAGNFTTSTATYHKDGLAPGTYYIRIRTFYSYEYTSYQLSDSLGLPIYAND
                     ATPNETYSAATVLPLNDTITGHIGYRYNGNIDAVDWYSVTTNADGRLYWTITVDNGQN
                     VYAELFDGDGTTNLGGSYTTTTATFNANGLAPGMYYIRIKTFYAYQFASYQLSNSLVE
                     PIQANDALPNDIYTTATPLTLNDSTTGHIGYRYNGSVDVYDWYAVTTNADGQLFWTIT
                     SHNGQYVYAHLFDGDGITLLAGNYTSTTATFNENGLAAGTYYIRIKTYYGYEFAPYTL
                     SNSLTPALYTNDTEPNGSVATAVPLAVNTTVTGHSAFKKQQPVGNKFYTVGIRC"
     gene            2173..3729
                     /gene="nadB"
                     /locus_tag="PKX59_07760"
     CDS             2173..3729
                     /gene="nadB"
                     /locus_tag="PKX59_07760"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOZ90799.1"
                     /translation="MTKTDFLVIGSGIAGLSFALKAAKHGKVTIITKANEDESNTKYA
                     QGGIAAVLPEAKDDSLEHHVQDTLSCGAGLCDEKIVRMVVAESGERINELIAWGTEFD
                     KNKSGNFDLAMEGGHSAHRVLHHKDITGLEIETKLLQQIHQHPNITLHTHLFAVDIIT
                     QHHMGVIVKRNTPGIACYGAYILNTKTGKVETMLAKVTVMATGGAGHVYKTTTNPVIA
                     TGDGIAMTYRAMGKVNGMEFIQFHPTALYHPGTYPSFLISEAVRGFGAVLRTIDGKEF
                     MQRYDERGSLAPRDIVARAIDSEMKKSGDDYVWLDCTKLNITEFKKHFPNIYNRCMSL
                     GIDVAKDCIPVAPAQHYLCGGIKVDEHGQSSIQHLYAIGECSSTGLHGGNRLASNSLL
                     EAVVFAHRAYEHASERLEFIHYNEHIPEWNSVSPREAAEMVLITQSRKELQEIMSSYV
                     GIFRSDMRLKRALERTRLLYEETEDLYNRWEISPRICELRNLINVAYLIIKAAMARTE
                     NKGLHFNVDN"
     gene            3745..4710
                     /locus_tag="PKX59_07765"
     CDS             3745..4710
                     /locus_tag="PKX59_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HOZ90800.1"
                     /translation="MISIFENNFNFENTALESFRFQAENNAVYRNYLKLLNIKPNQIS
                     NVSQIPFLPISFFKTKKVVSGNNDSKAIVFSSSRTTGAEPSLHYVNDISLYEKAFTQT
                     FNQYYSSADQWCILALLPSYLEREGSSLVYMCQHLIERTGKKNSGFYLNDFRKLADTI
                     IVNEQKGIKTLLIGVSFALLDFAHTHPMPLKNTIIMETGGMKGRRKEMIRQELHTILM
                     EAFQLNAIHSEYGMTELLSQAYSSGNGIFKCPPWMKVFVRDSNDPLSESKPGQGGINI
                     IDLANINSCSFIATQDIGRVHADGTFEILGRFDNAEVRGCNLLIE"
     gene            4724..5071
                     /locus_tag="PKX59_07770"
     CDS             4724..5071
                     /locus_tag="PKX59_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOZ90801.1"
                     /translation="MEKVTNLDFIKGFSKGDMSRVKKYVMMYIQSADEEMPVIEKSFN
                     DKDYKKLKTSSHTLKSIAGYMGMDHAQQLLKKIEDSASNESVTEEMSHDIAQLKEMIT
                     LSKDELKAFIAQN"
     gene            5108..5626
                     /locus_tag="PKX59_07775"
     CDS             5108..5626
                     /locus_tag="PKX59_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ90802.1"
                     /translation="MSKSSTIFIIDDDPMMQSMLKDHLSAMSNFTIHTFATGEEALAS
                     GQLVPDIVFLDYNLDSKIANAMDGIDVLREIKSKYPATEVVMLSGQDKIEVAVDTMKY
                     GAFDYIVKGESAFYRAEKTVFNLFKLAQLKKNAGMYKILTITFGAALLLMIILVIILK
                     AKGMITDHVGWY"
     gene            5639..6124
                     /locus_tag="PKX59_07780"
     CDS             5639..6124
                     /locus_tag="PKX59_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90803.1"
                     /translation="MKTVLALLLIFSLTSCRKEEKYPIEPVLLYKSVQFYEKNSFYLT
                     TTFTDGDGDIGLSDADTLPPYDQTSHFYHNYFCNYQQLINGSWTPINFSIPYYYRIPM
                     LTPKQRNKNIKGDIQVDVKNFLSFTTNGIADTFRVEISVADRALHESNKVLSDAFVIY
                     K"
     gene            complement(6166..8934)
                     /gene="leuS"
                     /locus_tag="PKX59_07785"
     CDS             complement(6166..8934)
                     /gene="leuS"
                     /locus_tag="PKX59_07785"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOZ90804.1"
                     /translation="MDYQFRETEKKWQQYWAANKTFKVENKSDKPKFYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRLKGFNVLHPMGYDSFGLPAEQYAIQTGQHPAITTET
                     NINRYREQLDKMGFSFDWDREVRTSDPSYYKWTQWIFLKLFDAWYNNKSNRAESIDKL
                     ISEFEKNGNNDISAVHDEIENFTAAQWKNFDETEKSRILMAYRIAYLGEAYVNWCPAL
                     GTVLANDEVKDGLSERGGHPVERKLMKQWSMRITAYAERLLQGLENIDWSDSLKEAQR
                     NWIGKSEGTTIRFSLQNSDDYIEVFTTRPDTLFGVSFVTLAPEHELVDKITTSSQRAE
                     VQDYVTKTKNRSERERQAEVTRISGVFTGSYVIHPFTGNKIPVWIGDYVLAGYGTGAV
                     MAVPAHDERDFRFATHLGLDRPQVIEPNITHDFGKGSWDAKEGKLINSGFLNDLDVKS
                     AISCCITEIEKQNLGKGKTNYRLRDAVFGRQRYWGEPIPIYYKENIPVAIPENELPLV
                     LPEVDKFLPTETGEPPLARAKNWKYKNLFPLETTTMPGWAGSSWYFLRYMDANNNTSF
                     ASKDALNYWQNVDLYIGGSEHATGHLLYVRFWTKALKDLGYINIDEPAQKLINQGMIQ
                     GTSCMVHREVATNKIISVGLKSNYNTTLIHIDVNLVDNTEVDIEGLKKWRDDFATAEF
                     VTENGKLICETAVEKMSKRWYNVVTPDSICEKYGADCLRMYEMFLGPLEQSKPWNTNG
                     IAGVSNFIRKLWKLYHNNGKFEVSNETATKAELKTLHKTIKKVQEDVEKFSFNTSVST
                     FMICVNELTEHKCNKREVLEPLAIILSPYAPYIAEELWQLLGHSNSVSFATFPEWNET
                     YLIENEFDYPVSVNGKTRFKIQLPLSIDKEEIERHVMASEEMQKWTNGNVPKKVIVVP
                     GRIVNVVI"
     gene            complement(8993..9763)
                     /locus_tag="PKX59_07790"
     CDS             complement(8993..9763)
                     /locus_tag="PKX59_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOZ90805.1"
                     /translation="MSLDLFYRSSGNGQPLIILHGLFGLNDNWQSFSKQITENNIAVY
                     AVDLRNHGSSPHDSVFDYDSMADDVFAFISKHDLQNVNIMGHSLGGKVAMKFALKHQD
                     ILKSLIVVDIAPRYYAPHHQQIIKALQAVDLDTVNTRKEAEHILSGYIDDAGVKMFLL
                     KNLYWEGDRLQWRFNLKVIAEKIESVGERITSTEKFEGKTLFIKGEKSKYITDDDKSE
                     ILNLFPEAEIKTCANAGHWVHADNPECMLTTVRDFLSQ"
     gene            complement(9763..12204)
                     /gene="pheT"
                     /locus_tag="PKX59_07795"
     CDS             complement(9763..12204)
                     /gene="pheT"
                     /locus_tag="PKX59_07795"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOZ90806.1"
                     /translation="MKISLDWLKDYIKLQQTPEEIEHLLTSSGLEVESLDETDSIKGG
                     LRGLVVGEVLEKEKHPDADRLSVTKVNVGKTIPLQIVCGAANVAKGQKVLVALNGARL
                     YPTFGEPFDIKNSKIRGQASEGMICSEDEIGLGNSHEGIMVLDANAVPGTPAAEWFKI
                     KQDHIFEIGLTPNRIDAASHGGVARDLAAVINTRAKAENNNSTTAQFSMPDVGAFKSE
                     NNALRIDVVVDKNSGCGRYCGVSISGVTVGESPEWLKSRLQSIGVKCINNIVDVTNFV
                     MFETGQPLHAFDAARVKGKIIVQKTGKASVFTTLDHIERKLTGDEVMICNATEPLCIA
                     GVMGGTDSGVSASTTQIFLESAWFNAAEVRKAAKHHGLKTESSFRFERGTDPNGTLYA
                     LKRAALLIKEVAGGKITSDVVDLYPEVVEPRIIYVNFEKINKLSGINFSNDMLLSVLE
                     YCGFSIKSSNVNGATISIPTYKTDVTRQADVVEEILRTYGYHHIPLNEKTGISFSSHT
                     PDRKPAMQYKISHHLSSIGFFEMINNSLVSSSHSSAGDEAVKVLNPLSKELDILRPDM
                     VASTLHTICYNINRKNHNLKLFEFGKTYLQHEGKLKETNNLVIAMTGMVNEPNWHKSE
                     KESSLFYLKSVAEKAIELVMPRISSHLNWVQDSHTLFSTFANIMQRQSAFGYIGSVKK
                     NILKQFDIAQPVYLAVLNIDVLLEKSLSHKTAIKLPQPFPEVKRDLSLMLDQAINYAD
                     LRDLALKQEKKLLKQVDIFDVYEGEKIEAGKKSYALSFTLADDEATLTDKQIESVMQK
                     IEKAFEKNLGAVIRK"
BASE COUNT         3774 a         2469 c         2276 g         3924 t
ORIGIN      
        1 atttcacact taaaaaccaa attattatga caactaaaat cgcaaaaaca aaaaggttgt
       61 ttcacttatc aagaataacc tttttcttgc tatgcttgtt cattacaggc ttagcaaaag
      121 ccgaaacaga gcctaatgac agcaaggctc aggccaacac attaacatta aatacaggaa
      181 tgcccggtac tgttgcactc agtgaatctg actggttttc tgttaccact ccggaagatg
      241 gaaatctgaa tatcaccggt actttttcga gttacattta tattcaggtt tacgataatg
      301 atggtacaac taatctcttt ctcaattatc attctggaac cgtaaccaga acagtttatg
      361 gcttggcagc aggcacctat tatgtgcgac tatacaatta cactgctacg gaaaccaaca
      421 cttatacgat aacaccaagc ctgacagcaa cgggctttgc caacgacaca gaggataaca
      481 gcactcctgc attagcaaat gtgttacctc tgaatggaag tgttaccgga catgttggct
      541 actattacaa caaccaacgc gatactatag actggtatgc tgttaccgtt actcaggatg
      601 gcactttaag ctacactatt acaagtgaaa atggtttata catttatgct aaactttatg
      661 atggtgatgg cacaacccaa cttgcaggaa attatacagg aagtactgca actttctcca
      721 gaggtgatat ggcgcctgga gtatattata tattaattca tgcatactac cctaacagtg
      781 aatatgtacc atatacacta tccaatacat taatacctcc tgttagtgca aatgacccct
      841 taccaaatga aactttttca actgcaacac cgcttgcttt aaatggcagt acaacaggtc
      901 atattggata tcgctacaac ggaaatgttg acgtttatga ctggtatagt gttaatacaa
      961 atgctgatgg agacttatac tttactataa ccagtgataa tggccagtat gtttacgcag
     1021 aattattcga tggtgatggc attacaaact tagccggaaa ctttacaaca tctactgcaa
     1081 catatcataa agatggttta gcaccgggca cttattatat tcgtatcaga actttttact
     1141 cttatgaata tacttcttat caattatctg acagtttagg tttgccgatt tatgctaatg
     1201 atgcaacacc gaatgaaact tactcagcag caactgtact ccccttgaac gatacaatta
     1261 caggccacat tggatatcgt tataacggaa atattgatgc tgttgactgg tacagtgtga
     1321 ctaccaatgc tgatggacgt ttatattgga ctattacagt tgataacggt caaaatgttt
     1381 atgcagaact atttgatggt gatggtacta ctaacttagg tggaagttat acgaccacaa
     1441 cagccacttt caatgcaaat ggtctggcac cgggcatgta ctacattcgt attaagactt
     1501 tctatgccta tcaatttgca tcctatcagc tatcaaatag tttagttgaa cctattcagg
     1561 caaatgatgc tttgccaaat gacatttata caacagctac accacttaca ctaaatgact
     1621 caactaccgg tcatattggt tatcgctata acggaagtgt tgatgtttat gattggtatg
     1681 ctgtaactac caatgcagac ggtcaactat tctggacaat aacttcgcat aacggacaat
     1741 atgtttatgc acatcttttt gatggagatg gaataacatt gctagccgga aactatacat
     1801 caactactgc cacttttaat gaaaacggac ttgctgccgg cacttattat ataagaatta
     1861 aaacatacta tggatatgaa tttgcaccat atactttaag caattcactt acgcctgcgc
     1921 tttatactaa tgatacagaa ccaaacggca gtgttgctac ggcagtccca ttagcagtta
     1981 atacaactgt aaccggacac agtgccttca aaaagcaaca acctgtcggc aacaaatttt
     2041 acactgttgg gattaggtgt taactcggca tagacctgat gatttcttgt tgtcatgaat
     2101 agttactttt gcaattaatt tacgaaagta aacatattcg acttatttta gtttaaaatt
     2161 tcgattaata aaatgacaaa gaccgatttt ctggtgatag gcagcggaat agccggctta
     2221 agtttcgcat taaaggcggc caagcatggc aaagtaacca tcatcacaaa ggctaatgaa
     2281 gatgaatcta acaccaaata tgcacaaggc ggcattgcag ccgttttgcc ggaagcaaag
     2341 gacgactctt tagaacatca tgttcaggat acgttgagtt gtggtgcagg attgtgtgat
     2401 gagaagatag tgcgcatggt agtagccgaa agtggcgaac gcataaatga actcattgcc
     2461 tggggaacag aatttgacaa gaacaagagt ggcaactttg atttagctat ggagggtggt
     2521 cattccgctc atcgtgtatt gcatcataaa gacattaccg gacttgaaat tgaaacaaag
     2581 ctgcttcaac agatacatca gcatccaaac atcacattac atacccatct ttttgctgtt
     2641 gacatcatta cgcaacatca catgggtgtt attgttaaac gtaatactcc gggaatagca
     2701 tgttatggtg catatatttt aaatactaaa accggcaaag tagaaactat gcttgcaaaa
     2761 gttacagtga tggctaccgg tggtgcagga catgtttata agactacaac caatcctgtt
     2821 attgcaaccg gtgatggcat tgccatgacc tatcgtgcca tgggaaaagt aaatggcatg
     2881 gagtttattc aatttcatcc cacagcattg tatcatcccg gaacctatcc atcttttctc
     2941 atttcagagg cagtgcgtgg ttttggtgca gtgttgcgaa ctattgatgg caaagaattt
     3001 atgcaacgct atgatgagcg tgggtcgctt gcgccacgcg atattgttgc ccgtgcaatt
     3061 gatagcgaaa tgaaaaaaag tggtgacgat tatgtttggc ttgattgtac aaaattaaat
     3121 ataacagagt ttaaaaagca tttcccgaat atttacaacc gatgcatgtc gttgggcatt
     3181 gatgttgcta aagactgtat tcctgttgca ccggcacaac attatttatg tggaggtatt
     3241 aaagttgatg agcatggaca atcgtctatt caacatcttt atgcaattgg agaatgttcg
     3301 tcaaccggac tgcatggtgg aaatcgtctg gcgtcaaatt cattactcga agctgttgta
     3361 tttgcgcatc gtgcttatga gcatgcctca gagcgattgg aatttatcca ctacaatgaa
     3421 cacattccgg agtggaattc agtttcgcca cgcgaagcag cagaaatggt tcttattaca
     3481 caaagccgaa aagagttgca ggaaataatg agcagctatg ttggaatttt cagaagtgat
     3541 atgcgactaa aacgggcgct tgaacgtaca cgacttttat atgaagagac agaagacctg
     3601 tataacagat gggaaatttc tcctcgtata tgcgaattgc gcaacctcat taatgtagca
     3661 tacctgatta ttaaagcagc aatggcaagg acagaaaata aaggactgca ttttaatgta
     3721 gataattaaa ttaattgctt ctgtgtgatt tcaatttttg aaaacaattt taattttgaa
     3781 aatacagcac ttgaaagttt taggtttcag gcagagaata acgctgttta tcgcaactat
     3841 ctgaaattat taaacatcaa gccaaatcag ataagtaatg ttagtcagat tccttttctg
     3901 ccaataagtt tttttaaaac aaaaaaagta gtctcaggaa ataatgactc aaaagcaata
     3961 gttttttcaa gtagcagaac tacaggtgca gagccatcac tacattatgt caatgatatt
     4021 tctttatacg aaaaggcttt tacacaaaca tttaatcagt attattcgag tgcagaccaa
     4081 tggtgtatat tagctttatt gccttcttat ctggagcgcg aaggttcatc gctggtgtat
     4141 atgtgtcaac atcttattga aagaacagga aagaagaaca gtggatttta tcttaatgat
     4201 ttcagaaaat tggcagatac cattatagta aatgaacaaa agggaattaa gactttactt
     4261 atcggtgtaa gttttgcatt gcttgacttt gcccatacac atccgatgcc actaaagaac
     4321 acaattatca tggaaacagg aggtatgaaa ggacgaagaa aagaaatgat aaggcaagaa
     4381 ctgcatacta tcctgatgga ggcgtttcag ttgaatgcaa ttcattcaga atatggaatg
     4441 acagaactgc tttcgcaggc atatagtagc ggtaacggta tttttaaatg cccgccatgg
     4501 atgaaagttt ttgtgcgcga cagcaacgac ccactgtcgg aaagcaaacc cggacaaggt
     4561 ggaatcaaca ttattgatct ggcaaatatt aacagttgta gttttattgc aactcaagat
     4621 attggaaggg tccatgctga cggcactttc gaaattctcg gcaggttcga taatgcagaa
     4681 gtgcggggat gcaatctttt aattgaataa ctatttttgc agtatggaaa aagttaccaa
     4741 cctcgatttt attaaaggat tcagcaaagg cgacatgagc cgcgttaaga agtatgtcat
     4801 gatgtatata cagagtgctg atgaggaaat gcctgttatt gaaaaatcat ttaatgataa
     4861 ggattataaa aaactcaaaa catcctcaca taccctaaaa tcaattgccg gctatatggg
     4921 aatggatcat gcacagcaac tgttaaaaaa aattgaagat agtgcatcta atgagagtgt
     4981 aactgaagaa atgtctcatg atattgcaca acttaaggag atgataactt tatcaaaaga
     5041 tgaactaaaa gcgttcatag ctcaaaatta atttgtattc ttgtcatgct aatactactg
     5101 tcataatatg tcaaaaagct caacaatatt tatcattgat gatgacccaa tgatgcaaag
     5161 tatgcttaaa gatcatctgt cggcaatgtc aaactttacc atccacactt ttgccacagg
     5221 tgaagaggca ttggcttccg gccagttagt tcctgatatt gtatttcttg attacaatct
     5281 cgattctaaa attgctaatg caatggatgg aatagatgtt ttaagagaga taaaatcaaa
     5341 atatcctgcc acagaagttg tcatgctttc aggtcaggat aaaattgaag ttgctgttga
     5401 caccatgaag tacggtgcat tcgactatat tgtgaaaggt gaaagtgctt tctaccgtgc
     5461 agaaaaaaca gtgttcaatc tatttaagtt agcacaactg aaaaagaatg ccgggatgta
     5521 caagatttta accatcactt ttggtgctgc attgttactc atgattattt tagtcatcat
     5581 tcttaaagct aaaggaatga ttactgatca tgttggctgg tactaatatc acaaaaccat
     5641 gaaaactgtt cttgcactct tattgatttt ctcacttaca tcatgccgta aggaggaaaa
     5701 atatcctata gagcctgttt tgttatataa gtcagtgcag ttttatgaga aaaacagttt
     5761 ttatctcacc acaactttta cagatggcga tggagatatt gggttaagcg atgcagatac
     5821 tttgccacct tatgaccaga caagccattt ctatcataat tatttttgta attatcagca
     5881 actcatcaac ggcagttgga cgccaataaa tttcagcatt ccctattatt accgcattcc
     5941 aatgcttaca ccaaaacagc gaaacaagaa tatcaaagga gatattcagg ttgatgttaa
     6001 aaattttcta agtttcacaa cgaatggaat tgccgacaca ttcagagtag aaatttctgt
     6061 tgccgacagg gcactgcatg aaagtaataa agtgctttca gatgcttttg taatatataa
     6121 gtaacaaaat atttcgaggg cgtaaatgca ggttaactca aaaggttaaa ttaccacatt
     6181 aacaattctt cccggaacaa caataacttt ctttggtaca ttgccatttg tccatttctg
     6241 catttcttca gaggccatca catgtctttc tatttcttcc ttatcaatac ttaaaggcaa
     6301 ttgaatttta aatctggttt ttccatttac agaaacagga taatcaaatt cgttttctat
     6361 taagtacgtt tcattccatt ccggaaaagt tgcaaaagaa acagagttac tgtggccaag
     6421 cagttgccat aattcttctg caatgtatgg cgcatagggt gaaagaatta ttgctaaagg
     6481 ttcaagtact tcgcgcttat tgcatttatg ttctgtcagc tcattcacac aaatcataaa
     6541 tgtacttaca gaagtgttga atgaaaattt ctcaacatct tcctgaactt tttttatagt
     6601 tttatgaagc gtttttaatt ctgcctttgt agcggtttca ttacttactt caaatttacc
     6661 attgttgtga tacagtttcc aaagctttct tataaaattt gatacgcctg caataccatt
     6721 ggtgttccat ggtttgcttt gttcaagagg tcccagaaac atttcataca ttcgtaagca
     6781 atcggcaccg tatttttcac aaatcgagtc tggtgttacg acattatacc aacgtttact
     6841 cattttttca acggcagttt cgcaaatcag ttttccgttt tctgttacaa attcagcagt
     6901 tgcaaaatca tctctccatt tttttaaacc ttcaatatca acttctgtgt tgtccaccag
     6961 atttacatca atgtgaatga gtgtagtatt gtaatttgat tttaatccta ccgaaataat
     7021 tttatttgta gcaacttcgc gatgtaccat acaagatgta ccttgtatca ttccctgatt
     7081 gattaatttc tgtgcaggct catcaatatt tatatatcct aaatctttta atgcctttgt
     7141 ccagaaacgt acatacaata aatgtcctgt tgcatgttcg ctgccgccaa tatagagatc
     7201 aacattctgc caatagttca aagcatcttt agaggcaaaa gaagtgttgt tgtttgcatc
     7261 catataacgt aagaagtacc aactgctgcc ggcccagcca ggcatagttg tagtttctaa
     7321 tggaaacaga tttttgtatt tccagttttt agctcttgcc aatggaggtt caccggtttc
     7381 tgtcggtaaa aatttatcta cttcaggtag gactaaaggc aactcatttt caggaattgc
     7441 aacaggaata ttttctttat aataaattgg aataggctcg ccccaataac gctgccggcc
     7501 aaacacagca tcacgcaggc ggtagtttgt ttttccttta cccagatttt gtttttcaat
     7561 ttctgttata caacagctaa tggcagattt cacatccaaa tcatttaaaa aaccggagtt
     7621 aattaatttt ccttccttgg catcccatga acccttaccg aagtcatgtg ttatattggg
     7681 ttctatcact tgtggtctat cgagtcccaa atgtgtagca aaacgaaagt cgcgttcatc
     7741 atgtgcaggc acggccatca ctgcgccagt gccgtaaccg gcaagtacat agtcgccaat
     7801 ccaaacagga attttattgc ctgtaaaagg atgaatgaca tagctacctg taaacacacc
     7861 actgattcgt gttacttcag cctgacgttc gcgctcactt ctgttttttg ttttggtaac
     7921 ataatcctgc acttctgcac gttgtgatga tgttgttatt ttatcaacca actcatgttc
     7981 cggtgctaat gttacaaacg aaacaccaaa taatgtgtcg ggacgtgtag taaatacttc
     8041 aatatagtca tcagaattct gcaaagaaaa tctgatggtt gttccttctg attttcctat
     8101 ccagttgcgc tgtgcttctt tcaatgaatc gctccaatca atattttcta atccctgcaa
     8161 caaacgttca gcataggctg ttatgcgcat gctccattgc ttcatcagtt tacgctctac
     8221 cggatgacca ccacgttcac tcaatccgtc ttttacttcg tcattggcca atacggtgcc
     8281 taatgcagga caccagttta catacgcttc gcctaagtat gcaatgcgat aggccatcag
     8341 tatccggctt ttttcagttt catcaaagtt tttccattgt gcagcagtga aattttctat
     8401 ctcatcatga acagcactaa tatcattatt gccgtttttt tcaaattctg aaatcagttt
     8461 gtcaattgat tcggcacgat tacttttatt gttataccat gcatcaaaca attttaaaaa
     8521 aatccattga gtccatttgt agtaggaggg gtcagaggtg cgcacttctc tgtcccaatc
     8581 gaatgaaaaa cccatcttat ccaactgttc acgatagcga ttgatgttgg tctcagtggt
     8641 aattgccggg tgctgacctg tctgtattgc atactgttct gcaggcaatc caaaagaatc
     8701 atatcccatt ggatgcaaaa cattaaaacc tttcagtctt ttatagcgtg aataaatatc
     8761 agaggcaata taacctaacg gatggccaac atgtagcccg gcaccggaag ggtaggggaa
     8821 catatccaac acataaaact ttggcttgtc agatttattt tcaaccttaa aagttttgtt
     8881 tgcagcccaa tattgctgcc attttttttc tgtttccctg aattgatagt ccattatttg
     8941 cgctccgaaa tgatgcgcta aaatactgaa atactgctga tgtttgtatt ctttattgac
     9001 ttaaaaagtc acgcaccgtt gtaagcatac attccgggtt gtcggcatga acccaatggc
     9061 cggcattggc acaggttttt atttctgctt cagggaataa gtttaatatt tcagacttat
     9121 catcatcggt tatatacttc gatttctcac ctttaataaa taaggttttc ccctcgaatt
     9181 tttctgttga tgtaattctt tcgcctaccg attcaatttt ttctgcaatt actttcagat
     9241 taaaacgcca ttgtaaacga tcgccttccc agtataaatt tttaagcaaa aacattttta
     9301 ctccggcatc atcaatatat ccagacagaa tatgttcggc ttcttttcgg gtgttaaccg
     9361 tatcgagatc tactgcctga agtgctttta ttatctgctg atgatgtggt gcataataac
     9421 gtggtgcaat atcaacaact atcaaagatt ttaaaatatc ctgatgcttc aaagcaaatt
     9481 tcattgctac tttgccacca agagaatgcc ccatgatgtt tacattttgt aaatcgtgtt
     9541 tactgataaa agcgaaaaca tcatcagcca ttgaatcgta atcaaacaca gagtcgtgtg
     9601 gcgaacttcc atgattgcgc aagtcaacgg catacactgc tatattattt tccgttattt
     9661 gttttgaaaa agattgccag ttatcattca gcccgaataa accatgcaaa ataattaacg
     9721 gctgaccatt accactgctg cgataaaata aatcaagcga cattattttc tgattactgc
     9781 tccgagattt ttctcaaaag ctttttcaat cttctgcatt actgattcta tttgtttatc
     9841 cgttaatgta gcctcatcat cagccaaagt aaaactcaat gcatacgatt tttttcctgc
     9901 ttcaattttt tcgccttcat acacatcaaa gatgtcaacc tgtttcaata atttcttttc
     9961 ttgctttaat gctaaatctc tcaggtcggc ataatttatt gcctgatcga gcattagtga
    10021 aaggtcgcgt tttacctccg gaaatggttg tggtaatttg atagcagttt tatgcgataa
    10081 acttttttcc agcaaaacat ctatattcaa tactgcaaga taaacgggct gtgcaatatc
    10141 gaattgtttc aaaatatttt tctttactga tccaatatac ccaaatgctg actgcctttg
    10201 catgatgttt gcgaaagttg aaaacaaagt atgactatcc tgcacccaat ttaaatgaga
    10261 gcttatgcga ggcatcacta actcaatagc tttttctgca acagatttaa gataaaataa
    10321 tgacgattct ttttctgact tatgccagtt aggttcattc accataccgg tcatagcaat
    10381 taccaaattg tttgtttctt taagttttcc ttcatgctga agataggtct taccaaattc
    10441 aaacaatttt aaattatgat tttttctatt gatattataa caaatagtgt gtagggtaga
    10501 agcaaccata tcggggcgca atatgtctaa ctccttactt aacgggttta aaactttcac
    10561 agcctcatca ccggcagatg aatgtgagga tgatactaaa gaattattaa tcatttcaaa
    10621 aaacccgata gagctcagat gatgtgaaat tttatattgc attgcaggtt ttctgtcagg
    10681 ggtgtgactt gaaaaggata tacccgtttt ttcattcaat ggaatgtgat ggtaaccata
    10741 agtccgtaaa atttcttcaa caacatcggc ctgtcgggta acatcggttt tatatgtcgg
    10801 tatggaaatg gttgcaccat ttacattgct gcttttaata ctgaagccac aatactccaa
    10861 cacagaaagc aacatatcat tactgaaatt aattcctgat agcttattta ttttttcaaa
    10921 gtttacatat ataatgcgtg gttcaacaac ttcaggatac aggtcaacaa catcggatgt
    10981 tatttttccc cctgcaactt cttttataag taaggctgca cgtttcaatg catatagtgt
    11041 tccattagga tctgtgcctc tttcaaaacg aaatgacgat tctgtcttta atccatgatg
    11101 tttggcagct ttacgcactt cggcagcatt aaaccaggca ctctctaaaa atatttgtgt
    11161 tgtagaagca gacacaccgg agtcggtgcc acccatcacc cctgcaatac acaatggttc
    11221 tgtggcatta catatcatca cttcatcacc tgtcagtttt ctttcaatgt ggtcgagtgt
    11281 agtaaataca gatgccttac ctgttttctg cacaataatt tttcctttca ctctggctgc
    11341 atcaaaggca tgcaatggct gcccggtttc aaacatcaca aagttggtta catcaacaat
    11401 gttgttaatg catttaactc ctatggactg cagtctgctt ttcagccatt ccggtgattc
    11461 gcctactgtt acaccactaa tagaaactcc gcaatatctt ccgcagcctg aatttttatc
    11521 tacaactacg tcaatgcgca gtgcgttgtt ttcagattta aaagcaccca catcaggcat
    11581 actgaattgt gctgttgttg aattattgtt ttctgctttt gctcttgtat tgattacagc
    11641 agcaagatcg cgtgccacac caccatgcga ggctgcatca atgcggtttg gtgtcagacc
    11701 tatttcaaaa atatgatcct gtttaatttt aaaccattca gcagcaggag tgcccggcac
    11761 agcattggca tctaaaacca taattccctc atgcgaattt ccgagtccta tctcatcctc
    11821 agaacaaatc atcccctctg aggcctgccc gcgtatttta gaattcttta tgtcgaatgg
    11881 ttcgccaaag gttggataca atcgtgctcc attcaaagca accaatactt tttgtccttt
    11941 ggcaacattg gcggcaccgc aaacaatctg caagggaata gttttgccta cattcacctt
    12001 cgtaacactc agtctgtcgg catctggatg tttttccttc tccaacactt cgccaacaac
    12061 caagccacgc agtccgcctt taatgctgtc ggtttcgtcc agcgactcca cttcaagtcc
    12121 gctgcttgtc agtaaatgtt caatttcttc aggtgtctgt tgtaacttta tatagtcctt
    12181 caaccagtcg agcgatattt tcattcttat ttttaatgaa agtgaaaatg caaaattaaa
    12241 ttattcgggc agacaaaaat attcagctat tatccctgct aaaggtcgct tccaaaaccg
    12301 ctgttatgct gatggtcttt tttgtcttgt tcatacttgc tgcagtctgt ctctatactc
    12361 agcggatgtt ccggtttttc aaaatcaccg ttgtacaaag tggaacgggt ggacgtttgc
    12421 gttttcgttt tggattgaca aat
//
LOCUS       DAOOOI010000058        12344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1001_length_12344_cov_36.879648, whole genome shotgun
            sequence.
ACCESSION   DAOOOI010000058 DAOOOI010000000
VERSION     DAOOOI010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12344
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1001_length_12344_cov_36.879648"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PKX59_07800"
     CDS             complement(<1..1006)
                     /locus_tag="PKX59_07800"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOZ90807.1"
                     /translation="MRRIIFCFLILFSVQGYAQQVLTNYTIANSPLPENSVRCIEVDH
                     IGRKWIGTDYGLAVFDDVNWTVYLPFASGLPANSVRAIAFDSLNNAWIGTLGGGVAKF
                     DGTNWTVYDDTNSPITENSIRALAFDSAGALWIGNQDGLVKFDGTNWIVYSAASSIIQ
                     NTIASIHTSKNRKVYAGSVNGGLFILHEDTIKSNLNISNGSGIPDNTILGFDEDSVGN
                     IWMATPANGIVVYRNIGGWFWYYMGSSFIQSNSLSDIQISNDHQSVWVATADSGIIRK
                     TGTVYTCFNTVNSSMPDNNIQCLTIDSLNNIWIGTATQGVVKFQDFTGIRNVSSVNDF
                     D"
     gene            complement(1062..5486)
                     /locus_tag="PKX59_07805"
     CDS             complement(1062..5486)
                     /locus_tag="PKX59_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HOZ90808.1"
                     /translation="MKRIYLLLVVLLFASQAIYAQCTLTNASTCSCLGGGTNCDLLPD
                     ITASRKPLTILGSNGVIEYSQSGNGVNDGRLRITVSTPNIGHGPMETRSSNIFVCGTD
                     TFVGTAPSICPDNITYPKIIINQRIYHKNGNSMSYYDRPAGTMTYHPSHGHQHVDNWG
                     VYTLRTPTADPNPVNWPIVGNGAKLAFCLLDIGACASSNGSGLCIADNGDTLDTPAEF
                     YGNYGLGGGSYGCSATLQGISAGYFDTYNQSLDGMWINIPPGTCNGTYYIVCQQDPDN
                     YFLEENDNNNVMAVPFTLTKQGGVAPTITANGPTTFCAGGSVTLTCSGASDYLWSNGA
                     TTQSITVSQGGTYTCTVNNTSTCATASTPVTVTVNSFPVTASTSSANVCAGDPVQLNG
                     SATSSGTVTVVQTFSNTTAYPIPDNNSTGVQSPITVSGINPTALSSGVVVSVNANITH
                     TYDGDIELRLVAPSGQYAVLSNRRGGSGDNYTNSTFAMNGPMAITSGNPPYNGTFIPE
                     GNFNSLTGNVNGVWKLHVIDRASVDVGTIQNWTLKINSQVPTSISYNWTSNPSGFNST
                     NQNPIANPTQTTTYTVSAVESTTGCAGSGSVTVNVANPNVSVSGSNTICEGTSTTLAA
                     NGATTYAWSPATGLSATTGSSVTASPTQTTTYTVIGMMNGCSDTTQFTVTVNPAPGTI
                     SATASSSPACIGDLVLLEAFNADVTSWSWTGPNNYSSTQSSNTILNIQPNQAGLYTVT
                     GSNACGSSSAQVNIVVNPTPYSQVTLTYPNGHCAGSYANACASDTSNGAYAPYTYLWD
                     NGQTTSCINQQILANPLPLQGPSVTITNSLGCTSTNIAVWNYPFGPSADSVQNPQTIC
                     QGGSYSINGNNYSTAGTYYDNFINVSGCDSIVITQLTVTPSLSINVSGNNTICEGQST
                     TLTATGASTYSWSPATGLNTSTGATVDATPAATTIYVVTGTSNGCSNTATFTVNVNPN
                     PVIQTSGNVDVCSGQFVTLSASGASNFTWSPSTALNTTTGASVVANPISTITYTVVGT
                     DNGCSASSSITVNIIAQPVVNVSASTTSICNGQSATLTASGADTYTWSPSTGLNTTSG
                     STVIATPSTTMTYTVTGFVAGGCFSTNTITINANTGTAPTIPGLIAGNKKPCPNVNEN
                     YSVSPVANAVSYTWTVPAGVTIISGQGTNNIHLNFASNFTSGNISVVANGACGVSASR
                     SSSLSLNIPATPSSITGPVTGLCDAAGTFTSATVSGATSYTWSVPAGVNIASGQGTKT
                     ASIYVGSSFTSGNVCVTADNTCMSSKPRCVAIKKTPSVPSTLTGPTTVCAGQQNVSYS
                     TNTVFGATNYQWIVPSGSVITSGQGTPNITVDFGTKTGSVGVNAYNACGQAGRRAVLV
                     SFNCRLGEMSSSTSVIEISPNPTSTGMVNLTVNSEVNGIALIKMTDILGKSVLIKNIA
                     NVIGTNNHKLDLSKFNKGIYLLTVDTGSKKQTLKVVIQ"
     gene            complement(5620..5790)
                     /locus_tag="PKX59_07810"
     CDS             complement(5620..5790)
                     /locus_tag="PKX59_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90809.1"
                     /translation="MQHLFRCYKSTGRKKIYLFGILRKIYTGFNGPATGAYHEKTKDD
                     FIRIITTLLSEK"
     gene            complement(5821..6210)
                     /locus_tag="PKX59_07815"
     CDS             complement(5821..6210)
                     /locus_tag="PKX59_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90810.1"
                     /translation="MDTAINNYSADYASNKFYENKYRQHYKLLEAKFPVNILTLDFDS
                     SVLKAAEIDSMLDVWFSPKYPFLDEPHNCEDNDICVEIRYSQNLKKFLYRGDYDPENY
                     DKVAFKTLTVSFFLKKNKQYYNEQKQH"
     gene            complement(6292..6648)
                     /locus_tag="PKX59_07820"
     CDS             complement(6292..6648)
                     /locus_tag="PKX59_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90811.1"
                     /translation="MRKLYLVISALIVFFSSCNSNSNQAISESYIPIFADTTGWFFSK
                     DNLVINLNIKYINDAYKFEKQLGSKISYLAQGVNFKTNFNVPTTYYDSIMSPKEIPAF
                     VIFHRPEKYEFPLNVI"
     gene            complement(6666..7271)
                     /locus_tag="PKX59_07825"
     CDS             complement(6666..7271)
                     /locus_tag="PKX59_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90812.1"
                     /translation="MLAKKSTIMFNNLFLFVSILFLANCRSDSKPQNISGILFKDSLN
                     TLSIYIPKRFSIVDSIYEKEILGDKFEFFSATFNGDSSKIDEFIKYCNSLIVGVEFNS
                     PVKSLAEIDSLDEHAPTFQVRIKYTLRTKLKSDKKNHFLIKQKFFKSSGEFNIQVVNA
                     YCLHKSDLFFLQSIFYYSNKAKTEKNYKEIIGVLESLEFIN"
     gene            complement(7237..8670)
                     /locus_tag="PKX59_07830"
     CDS             complement(7237..8670)
                     /locus_tag="PKX59_07830"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOZ90813.1"
                     /translation="MIEISQIKYLCFEGGGGKGLAYLGVIQALEEIINKKMGPPEGGT
                     PYNVTSENKTPIIRISQPIKQRNILGVSGSSAGAITAFMVAMGMSHYEILQETNKVVS
                     LSLGNFTIAQKDVSVTQFESFFDSPDLYNYRTNNKKSPLREYNNLSMPIKIIGNVLSE
                     FLNVFKWTFKPFVSENEIFVRKILLTEGTPARALNLKKYFLGIIYNRGLLSGVASKKY
                     FSDLIENYLFPKLSTLKMKKLKSRGEDITFQDFFYMTGVDLIVTGTNISLGKPKYFSA
                     AFTPDFSVVDAISLSMSIPFMFRPYLIESEVNLDSNLPADYNKDYLGLYVDGGMLNNY
                     PLHAFDYRPQKNINYQGNFDNYPLASPNISSSATEFCDCILGIRLQNLVKEYEVSDDF
                     KEGNSVIIKDFINQLYNTILYPSEEGQIRSVLENNLSVSIDVTDLELLDFSNPLINRQ
                     YRNLNSLAEKQEKVIENACKKIYDNVQ"
     gene            complement(8682..8918)
                     /locus_tag="PKX59_07835"
     CDS             complement(8682..8918)
                     /locus_tag="PKX59_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90814.1"
                     /translation="MYYLSSGGEIWNGEDVPSPTDPLYISIIDEMKEFDGQFVGGNQE
                     GEPWIQKAPTNLVYLTKLDGSSPGLPDFSDELLP"
     gene            9313..9591
                     /locus_tag="PKX59_07840"
     CDS             9313..9591
                     /locus_tag="PKX59_07840"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOZ90815.1"
                     /translation="MFAFCIMPSHIHLIAKTEDDVSLSNVLRNFKSYTSKQIIKMIEE
                     SHTESRREWLLYMFEFYGKKMRTMLNISFGNRIIMPSIYSVINLLIKK"
     gene            complement(10006..11178)
                     /locus_tag="PKX59_07845"
     CDS             complement(10006..11178)
                     /locus_tag="PKX59_07845"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOZ90816.1"
                     /translation="MSSTALHGLIIASGIWRAVLMTPGGELPFNFETREVNSRSEIFI
                     KNGNEEIEVNEISYANDSLIIQLPVYDSEFRVRVSGDSMIGFWYNNSRKNYAPTPFKA
                     FLGEKYRFFNNTKSEIKLPEKWEWKFSPGTKDSSQAIALFKQNGTDVRGTLLTESGDY
                     RFLDGCLNNDSLFLSYFDGAFAYLFKGSIKNNMMQGVFYSGHHWQQPWQAVVNESAEL
                     PDPFKLTFLKNDTASFSFSALRLDSTVFSYPNKDLKGRVVVVEMMGTWCPNCMDETAF
                     LSDYYSRNKSQDLTIIGLAFEKTSDFRKSVGNVKRVKEKYHVQYETLIAGNREGAASK
                     MSMLSKINGYPTTIFIDRKGKVRKIYTGFNGPATGAYHERTKDDFIRIITTLLSEK"
     gene            complement(11195..>12344)
                     /locus_tag="PKX59_07850"
     CDS             complement(11195..>12344)
                     /locus_tag="PKX59_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOZ90817.1"
                     /translation="ERPSRFKKEGGFEREKRSFKSSDSPRPYRKRDEGFEGDEKPRFK
                     KRSDFGGSGKFDKSKSFKRRDDNVEGEDKPRRFSKSFDSDTREKRSYDRKERSSGRFD
                     KPYRKKNDNRKYSKKEKESSEQLSDGLVRLNRYIANSGICSRREADELITAGLVSVNG
                     EIVTTMGTKVSQDDVIKYNGETIRNEKPMYLLLNKPKDYITTMDDPQDRRTVMHLIAG
                     ACKERVYPVGRLDRNTTGVLLFTNDGDLAKKLTHPSYEVQKIYHVELDRNLKSSDKEK
                     IKEGVMLDDGMATVDDIQYDGGFNDKKQIGVEIHSGKNRIVRRIFESMDYNVIKLDRV
                     VFAGLTKKDIARGKWRFLSEMEVNALKMATSKVKPKDKEPRKRISRSK"
BASE COUNT         3801 a         2305 c         2170 g         4068 t
ORIGIN      
        1 aatcaaaatc attgacggaa gaaacattac gtattcccgt aaagtcctga aatttaacaa
       61 caccttgtgt tgctgtgcct atccatatat tgttcaaaga atcaatagtc aagcattgaa
      121 tattgttatc aggcatggaa gaattaacag tgttgaagca agtataaaca gtacctgttt
      181 ttctgattat accactatcg gcagtagcta cccaaacaga ctgatgatcg ttactgattt
      241 gtatatctga aagactattt gactgaatga aagaacttcc catataatac caaaaccatc
      301 caccaatatt tctgtaaacc actattccat ttgctggagt tgccatccag atgtttccaa
      361 cagaatcttc gtcaaatccc aaaatagtat tatcaggaat tcctgaacca tttgaaatat
      421 tcaagtttga tttaatcgtg tcttcgtgaa gtataaacaa tccgccatta acacttcctg
      481 cataaacttt tctgtttttt gaagtatgaa tagatgcaat agtattttga ataatgctgc
      541 ttgctgcaga gtaaactatc cagttggttc cgtcaaactt tacaagtccg tcctgatttc
      601 caatccacaa tgcacctgct gaatcaaatg caagagctct gattgaattt tcggtaatgg
      661 gactattggt atcatcataa actgtccaat tagttccatc aaactttgcc acacctccac
      721 caagtgtacc aatccaggca ttgttcagtg agtcgaatgc aatagcacgc acactattag
      781 caggcaatcc tgatgcgaag ggaagatata ctgtccaatt tacatcatca aaaacagcaa
      841 gtccataatc ggttccaatc cattttcttc ctatatgatc aacttcaata catcttaccg
      901 aattttcagg taatggtgaa tttgctattg tatagttagt caacacttgt tgtgcatacc
      961 cttgcacaga aaataaaatc aggaaacaaa atattattct tctcattctt taaaaataaa
     1021 aaccctcccg taggagggca ttattccggt tttagatata attattgtat tacaactttt
     1081 aatgtttgtt ttttactacc ggtatcaaca gtcaacaaat aaattccctt gttgaatttc
     1141 gacaagtcta atttatgatt gtttgtacct atcacatttg caatgttctt tattaaaact
     1201 gactttccta aaatatcagt cattttaatt aaagctatac cattaacttc tgaattaact
     1261 gtcaggttaa ccattcctgt tgaagttgga tttggcgaaa tttcaataac tgaagtacta
     1321 ctactcatct cgcctaagcg gcaattgaat gaaacaagta ctgctcttct tcctgcttgt
     1381 ccacatgcat tgtatgcatt gacaccaaca cttcccgtct ttgttccaaa atcaactgta
     1441 atattaggag ttccctgacc tgatgtaatt actgatcctg atggaactat ccattgatag
     1501 ttggttgcac caaatacagt atttgtacta tatgacacat tttgttgtcc tgcacaaact
     1561 gttgttggtc cggttaaagt tgatggcact gatggtgttt tcttaattgc tacacaacga
     1621 ggcttgctac tcatacatgt gttgtcggct gtcacacata cattgccact cgtaaagctg
     1681 ctgcctacat aaattgaagc agtcttagtt ccctgaccac ttgcaatatt tactccggcc
     1741 ggaaccgacc atgtataact tgtggcccca ctaacagttg cacttgtaaa tgtgcctgct
     1801 gcatcacata atccagtaac cggacctgtg atagatgatg gtgtggcagg aatattcaat
     1861 gacagcgaag atgagcgtga agcactcaca ccgcaagcac catttgcaac aactgatata
     1921 ttaccacttg tgaaatttga tgcaaagttt aaatgaatat tattagttcc ttgccctgaa
     1981 ataatagtca cccctgcagg aacagtccat gtatatgaaa cggcattagc tacaggactt
     2041 acagaataat tttcattcac attcgggcaa ggctttttat ttcctgctat taaaccaggt
     2101 atagtcggtg cagtgcctgt gtttgcatta atggtaatgg tatttgtact aaagcaacct
     2161 ccggcaacaa aacccgtaac tgtgtaggtc attgttgtgg atggtgttgc aattactgtt
     2221 gaacctgatg tagtatttaa gcctgttgat gggctccatg tgtatgtatc tgctcctgat
     2281 gcagtgagcg tagcagactg accattacat atagatgttg tacttgctga aacatttacg
     2341 acaggctgag caataatatt cacagttatt gaagaagaag cagaacatcc attatctgta
     2401 cctacaactg tgtaagtaat cgttgatatt gggttagcaa caacgctagc acctgttgta
     2461 gtatttaatg ctgttgaagg cgaccatgta aagttgcttg caccactggc tgataaagtc
     2521 acaaactgtc cactgcaaac atctacattt ccacttgttt gaataacagg atttggattt
     2581 acatttactg taaaagttgc tgtatttgag cagccgtttg atgtgcctgt aacaacatag
     2641 atagttgttg cagcaggtgt agcatctact gttgctcctg ttgaagtatt taatcctgtt
     2701 gcaggactcc atgaataagt tgatgcacct gttgctgtta atgttgttga ctgtccttcg
     2761 cagatggtat tgtttccact aacattaata ctaagcgaag gtgtaactgt aagttgtgta
     2821 attactatac tatcacaacc ggatacattg ataaagttat cgtagtatgt tcctgcggta
     2881 gaataattat ttccattaat agagtaagag cctccctgac aaattgtttg tggattctga
     2941 actgaatcgg ccgaagggcc aaaaggataa ttccaaactg caatattcgt tgatgtacat
     3001 cctaaactat ttgtaatagt aacactcggg ccttgcaagg gtaatggatt tgcaagtatc
     3061 tgttgattta tacaagaagt tgtttgacca ttatcccata ggtaggtgta tggtgcatag
     3121 gctccgtttg atgtgtctga agcacaagcg ttagcatagc tgcctgcaca atgtccattt
     3181 ggataagtta atgttacttg tgaatagggt gtcggattta caacaatgtt aacctgagca
     3241 gatgaacttc cgcaagcatt gcttcctgtt actgtatata atcctgcctg attgggttga
     3301 atattaagta ttgtatttga agactgtgtt gaactgtaat tattcggccc tgtccaactc
     3361 caggaagtaa catcagcatt aaatgcttca agtaaaacaa gatctcctat acatgcaggg
     3421 cttgatgatg cagttgcaga aatggtgccc ggtgcaggat ttactgtaac agtaaattgt
     3481 gttgtatcag aacaaccatt catcattcca attacagtat aagttgttgt ttgagttggc
     3541 gaagctgtaa cgcttgatcc tgtagttgca cttaaacctg ttgcagggct ccatgcataa
     3601 gttgttgcac cgtttgcagc aagcgttgtt gatgtacctt cacagattgt attgcttcct
     3661 gaaacactaa cgtttggatt tgccacattc actgtcactg agcctgagcc tgcacatcct
     3721 gtagtactct caactgcaga tactgtatat gtagtagtct gtgtaggatt agctatcgga
     3781 ttctgatttg ttgaattaaa tccagaagga ttagatgtcc agttataaga tattgatgtc
     3841 ggaacctgtg aattgatttt caatgtccaa ttttgaattg ttcctacatc tacagatgca
     3901 cggtcaatta catgcaattt ccaaacaccg ttaacattac ccgttaagct gttgaagttt
     3961 ccttctggaa taaatgttcc attataaggt ggatttccac tggtaatagc cattggtccg
     4021 ttcattgcaa aagttgaatt ggtataatta tcaccactac cacctctgcg attactcagc
     4081 actgcatatt gtcctgatgg agcaactaaa cgtaattcga tgtcaccatc ataagtatgt
     4141 gtaatatttg catttacaga cactacaaca cctgaactta atgcagttgg attgatacca
     4201 ctaacggtta ttggtgattg tacgcctgtt gaattattat caggaatcgg ataagctgta
     4261 gtgttagaaa aagtttgtac aacagtaaca gttcctgaac ttgttgctga gccattgagt
     4321 tgcacagggt caccggcaca aacattagca gaacttgtag atgcagttac cgggaaacta
     4381 ttaaccgtta ctgtaaccgg agttgatgca gtagcacagg ttgaagtatt atttactgta
     4441 caagtgtaag ttccaccttg tgaaacagtg atgctttgtg tagtagcacc attgctccat
     4501 aaataatcgc ttgcaccgct gcaagttaaa gttacgctgc ctccggcaca aaatgttgtg
     4561 ggcccgtttg cggtaattgt tggagctacc ccaccttgtt ttgtcagtgt aaatggcact
     4621 gccattacgt tgttgttatc attctcttca agaaaataat tgtcaggatc ctgttgacat
     4681 acgatgtaat aggtaccatt acaagtaccg ggaggaatgt taatccacat gccatcaaga
     4741 ctctgattat aggtatcaaa ataacctgct gaaatacctt gcaatgttgc tgaacaacca
     4801 taacttcctc cacctaaacc atagttgcca taaaattctg caggtgtatc caatgtatcg
     4861 ccattgtctg caatacataa gccacttccg tttgatgatg cacaggctcc tatatcaagc
     4921 aaacaaaaag caagttttgc accattacct acaataggcc aatttacagg attaggatct
     4981 gctgttggtg ttctcaaagt ataaacgccc cagttgtcaa cgtgttgatg accatgtgat
     5041 gggtgatatg tcattgttcc tgcaggacga tcataatatg acattgaatt gccgttctta
     5101 tgataaattc tttggttgat gattatttta ggataggtaa tgttatcagg acaaattgat
     5161 ggcgctgtac caacaaaagt atctgtgccg caaacaaaaa tatttgacga gcgtgtttcc
     5221 ataggtccat gtccgatgtt tggtgtagaa actgtaattc tcaggcgtcc atcattaaca
     5281 ccgttgcctg actgcgaata ttctataaca ccgttggaac caagtatggt caatggtttt
     5341 cttgatgcag taatatcagg taataaatca cagttagtgc caccgcccaa gcatgagcat
     5401 gttgaggcgt tagttagtgt acactgtgca taaatggctt gactagcaaa caatagtaca
     5461 accagtagta ggtaaatacg tttcatatta gtttgattta aatattatgg gaggattaaa
     5521 aagtagccca aattaagcat ttttctgaaa cttgcaagtg gtttttttga ttaacagaaa
     5581 aaatctgtag tcaaaaagca gaaaaatata ggtgaacagc tatttttcgg aaagcaaggt
     5641 agtgatgatg cggataaagt catccttagt tttttcatga taggcacctg tagccggacc
     5701 attaaatcct gtatagattt ttctaagaat accaaacagg tagatttttt ttcttccagt
     5761 gcttttatag cacctaaaaa ggtgctgcat ttacctccac caccctcaaa tgacaagtat
     5821 ttaatgttgc ttttgttcat tataatattg cttgtttttt ttaagaaaaa aagaaactgt
     5881 tagtgtttta aaagccacct tatcataatt ttcaggatca taatcgcctc tatacagaaa
     5941 tttttttaaa ttctgtgaat atcgaatctc cacacaaata tcattatctt cacaattgtg
     6001 tggttcatca agaaatgggt attttggaga aaaccaaaca tcaagcatgg aatcaatttc
     6061 agcagctttt aaaacactgg aatcaaaatc taatgtcaga atgtttacag gaaactttgc
     6121 ttcaagtaat ttatagtgtt gcctgtactt attctcgtaa aatttattgc ttgcataatc
     6181 tgcggagtag ttattaatag cagtatccat atcccgttga gcgtaatgta tccaatatgg
     6241 cgcccattca tagtaaatat atgctacaat acgggtacgg gggttatatt cttaaattac
     6301 atttaaagga aattcgtatt tttcagggcg atgaaaaatt acgaacgctg gtatttcttt
     6361 aggactcatt atagaatcat aataagtagt tggtacatta aaattcgttt tgaagttgac
     6421 cccttgtgca agataactga ttttagaacc taactgtttt tcgaatttgt aagcatcatt
     6481 aatatattta atatttaaat taattactaa attatcttta gagaagaacc atccggttgt
     6541 atcggcaaaa ataggaatat aagattcact tattgcctga tttgaattag aattgcagga
     6601 actaaaaaaa acgataagtg ccgaaatgac aaggtagagt ttgcgcatgg tgtgatttgt
     6661 tgttgtcaat ttataaattc caaactttct aacacaccaa taatttcttt ataatttttt
     6721 tcggttttgg ctttatttga ataataaaaa atggactgca aaaaaaacaa gtcggatttg
     6781 tgaagacaat aagcattaac aacttgaata ttaaattctc ctgatgattt aaaaaatttt
     6841 tgctttatta aaaaatggtt ttttttatcg gatttgagct tagttcttaa agtatactta
     6901 attcttactt gaaaagttgg tgcatgttca tctaaactat caatctctgc taaacttttt
     6961 acaggtgagt tgaattcaac ccctacgatt aaagagttgc aatatttaat aaactcatca
     7021 atcttagagc tgtctccatt aaaagttgcc gaaaaaaatt caaacttatc cccaaggatt
     7081 tctttttcgt atatactatc cactatagaa aatcttttgg gaatataaat tgaaagggtg
     7141 tttaaagagt ccttaaaaag tatcccacta atattttgag gtttagaatc acttctgcaa
     7201 tttgccagga ataaaattga aacaaagaga aaaaggttat tgaacattat cgtagatttt
     7261 tttgcaagca ttttctataa ctttctcctg cttttctgca agactattta agtttctata
     7321 ttgacggttg attaacgggt ttgagaaatc aagcaattct aggtctgtaa catcaattga
     7381 aacggataaa ttattctcaa gaacggacct aatttgtcct tcttcgcttg gatatagaat
     7441 ggtattgtaa agttgattaa taaaatcttt tataataacg ctatttcctt ctttgaaatc
     7501 atcagacacc tcgtattcct taactaaatt ttgcagacga attcccaaaa tacagtcgca
     7561 aaattctgta gctgatgaac tgatattggg agaagctaaa ggatagttat caaaattgcc
     7621 ttggtagtta atattttttt gcggtctgta atcgaaggca tgtaacgggt agttgttgag
     7681 catacctcca tcaacataca gcccaagata atctttatta taatcagcag ggagatttga
     7741 atcgaggtta acttctgact cgattaagta aggcctaaac ataaaaggta tgctcatgga
     7801 taaactaatt gcatcaacaa cagaaaaatc aggtgtaaaa gcggcagaga aatattttgg
     7861 tttgcctaac gaaatattgg ttccggtaac gataagatca acaccagtca tgtaaaaaaa
     7921 atcctgaaaa gtgatatcct cacctctgga ttttagcttt ttcattttta gtgtggaaag
     7981 tttcggaaat aaataatttt caattaaatc ggaaaaatac tttttagagg cgacaccgct
     8041 tagtaatccc ctattataaa taatacctaa aaagtatttc tttaaattta atgctctagc
     8101 aggagtacct tcggtaagta atatttttct tacaaaaatt tcattctctg aaacgaaagg
     8161 cttaaatgtc cacttaaata cgttgaggaa ctcggaaagt acattgccta taatttttat
     8221 aggcattgaa agattattat actcccttaa tggcgatttt ttattatttg ttcgataatt
     8281 atataaatcc gggctatcaa aaaaactttc aaattgtgtt acggaaacat ctttttgagc
     8341 aatggtaaaa ttccccaacg ataaacttac aactttgttt gtttcttgca gaatttcata
     8401 gtgggacata cccatcgcaa ccataaaagc ggtgattgca ccagccgaag aaccactaac
     8461 tccaagaata tttctttgtt tgattggttg cgaaattcta ataattggtg ttttgttttc
     8521 agaagttacg ttatatgggg taccgccttc aggtggcccc atttttttat taattatttc
     8581 ttctaatgct tggattacac ctaaataggc aagcccttta ccgccacctc cttcaaagca
     8641 caaatattta atttgtgaaa tttctatcat agcaagagtt tttatggaag caactcatcc
     8701 gaaaaatcag gcagtccggg gcttgagcca tcaagttttg tgaggtaaac aagattggta
     8761 ggggctttct gaatccaagg ttcaccttct tgattacctc caacaaattg tccgtcaaat
     8821 tctttcattt cgtcaatgat agatatataa agcgggtcgg ttggtgaggg tacgtcttca
     8881 ccattccaaa tttcaccacc cgaacttaag taatacaaaa ttgtgactcg tcctaaaaaa
     8941 agtttacagt ttaattgatt aatcctgata taagtttaca attcattttt agtcctgaat
     9001 tgggtttaca atagtaggaa tttttcgata ataattctta cataattttt ctgttttgat
     9061 aattgttttt gaatgatgaa tacccgtagg tgcgagcgtc ctttccgctc gtgccattca
     9121 ttttgtttgc atcttgcaaa ctagtaagat ttactttgcc ggtgtatgag cgaaaaatac
     9181 aaaatatttc ctgacaagtt atactttgtt acattaacca cagttggtta gattgatgtt
     9241 tttacaagag ccatttactg cgatgagata gttaagaact taaattacag cattgacaat
     9301 aaaaagctaa aagtgtttgc tttttgcatt atgccatcac acattcattt aattgcaaaa
     9361 acggaagacg atgtgagttt aagcaatgtg ttacgcaact ttaagagtta cacatcaaag
     9421 caaattatta aaatgattga agaaagccac acggaaagca ggcgcgaatg gttgctttat
     9481 atgtttgagt tttacggcaa aaaaatgcgc acaatgttaa atatcagttt tggcaacaga
     9541 ataatcatgc cttcgattta ttcagtaata aatttattga tcaaaaagta aattacatac
     9601 acaacaatcc tgtagaaaca cgaatagtta cagagcccca tttttatgtg tatagcagtg
     9661 ctaatcaatt taccgattta aagctggttg aactatgaag tccgatagct atcggacggc
     9721 cagaagccac aaggataaaa ggcacgagcg gaaaggacgc tcgcgcctac ttagtaatag
     9781 caggaaacta ctcgaaatta aatttagaat atttcgaaag ttgaaatatt ctaaattttg
     9841 tgatatttct ttatagtcac ttgacataat atcttggaga atctgcgagg ataaagggaa
     9901 taaaaaagta acttaaatta agcctttttc ctgaaacttg caagtggttt ttttgattaa
     9961 cagaaaaaat ctgtagtcaa aaagcagaaa aatataggtg aacagctatt tttcggaaag
    10021 caaggtagtg atgatgcgga taaagtcatc cttagtcctt tcatgatagg cgcctgtagc
    10081 cgggccatta aaacctgtat agattttcct gactttgccc tttcgatcaa taaaaatggt
    10141 agtaggatag ccattgattt tactgagcat actcatttta gatgcagccc cttctctgtt
    10201 accggcaatc agtgtttcgt actgaacatg gtatttttct ttcactcgtt taacattacc
    10261 gacagatttc ctgaagtcag atgttttttc aaaagccaaa ccaattatgg ttaagtcttg
    10321 gcttttattt ctggaataat aatcggataa aaatgctgtt tcgtccatac agttagggca
    10381 ccatgttccc atcatctcaa caacaaccac cctacctttt aaatctttat tagggtatga
    10441 aaaaactgtg ctgtccaatc gcaatgcact aaaagaaaaa gatgcagtat cattcttcag
    10501 aaaagtgagt ttaaacggat cgggcaattc tgcactttca ttaactactg cttgccaggg
    10561 ttgctgccaa tgatgtcctg aataaaaaac accctgcatc atattattct ttattgagcc
    10621 tttaaaaaga taagcaaatg ctccgtcaaa ataagataaa aacaaactgt cgttattaag
    10681 acatccatca agaaaacgat agtcgcccga ctcagttaaa agtgttcctc tcacatctgt
    10741 accattctgt ttaaacaatg caattgcttg cgatgaatct ttagtacccg ggctgaattt
    10801 ccattcccat ttttcaggaa gttttatttc agattttgtg ttattaaaaa aacgatactt
    10861 ttctccaaga aaagctttga atggtgtggg tgcataattc tttcttgaat tgttatacca
    10921 aaagccaatc atgctgtcac cgcttactct cactctgaac tcagaatcat aaaccggtaa
    10981 ttgaataatt aaagaatcat tggcataaga aatttcattt acttcaattt cttcattgcc
    11041 attttttata aaaatctcac tgcggctatt aacctctctt gtttcaaaat taaaaggtaa
    11101 ttcaccgccc ggtgtcatta atactgctct ccatataccg gaagcaataa tcaaaccatg
    11161 caatgcagta ctactcaagt ttaattatat atagttattt acttcgcgaa attcttttgc
    11221 gtggctcttt gtcctttggc ttcacctttg atgtagccat cttcaaagca ttcacttcca
    11281 tctcactcaa aaaacgccat ttacctcgtg caatatcctt cttggtcaag cctgcaaaaa
    11341 ctacccggtc taatttaata acattgtaat ccatagattc aaatattcgt cttacaatac
    11401 ggtttttacc tgagtgtatt tctacaccaa tctgtttttt gtcattaaat ccaccatcgt
    11461 attgtatgtc atcaacagta gccattccat catcgagcat tacaccttct ttaatttttt
    11521 ctttatcgga agattttaaa tttctgtcca actctacatg ataaattttc tgcacttcat
    11581 aactaggatg tgtaagtttt tttgccagat caccatcatt ggtaaaaagc aaaacaccgg
    11641 ttgtatttct gtcgagtcgg ccaacaggat aaacacgctc tttacaagca ccggcaataa
    11701 gatgcataac agtacgtctg tcctgcggat cgtccattgt ggtgatgtaa tcttttggtt
    11761 tattgagcaa cagatacatt ggcttttcat ttctgatagt ttcaccattg tatttgataa
    11821 catcatcctg cgaaaccttg gttcccattg tagtaacaat ttcaccattg acagaaacaa
    11881 ggcctgcagt aatcaactca tctgcttcgc gtctgctaca aataccactg ttggcaatat
    11941 aacggtttaa tcgaacaaga ccatcagaca attgttctga tgattccttt tcttttttac
    12001 tatattttct gttgtcattt ttcttgcgat acggtttatc aaatcttcct gaacttcttt
    12061 cttttctgtc gtaagaacgt ttctctcttg tatcactatc gaaagatttg ctaaatcgtc
    12121 ttggcttatc ttctccttca acattatcat ctcttctttt aaaacttttt gacttgtcaa
    12181 attttcctga accaccaaag tcacttcgtt ttttaaatct tggtttctca tcaccttcaa
    12241 aaccttcatc tctttttctg taaggacgtg gactatctga tgatttaaaa cttcttttct
    12301 ctcgctcaaa accaccttct tttttaaagc gcgatggtct ttct
//
LOCUS       DAOOOI010000059        12193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1013_length_12193_cov_8.509474, whole genome shotgun sequence.
ACCESSION   DAOOOI010000059 DAOOOI010000000
VERSION     DAOOOI010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12193
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1013_length_12193_cov_8.509474"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PKX59_07855"
     CDS             <1..818
                     /locus_tag="PKX59_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOZ90818.1"
                     /translation="IKVTKSSNIYIPNNEVEKTFIKCIWRLSEYDIQERKEIILPKGT
                     VEIIFNFSDKINYFSPSYKIEKSLPDVFVNGINFKPFELTKTKQQKFLGVQLNSIGLR
                     LLFKIPSFELNNIVCDGNDICKQLSNLANDLNGEEEFSQQVKIILSWIRNTVSFNQIK
                     YATSRAEKLTSCICYNNLTVKKLSKEIGLSERQLSRFSNDWLGMSTEQFIHYRKYLNC
                     LNLLHQSKQNLTALGMQAGYYDQSHFIHQFKSFTDMTPRQYREAKTDLPGHIY"
     gene            902..1936
                     /locus_tag="PKX59_07860"
     CDS             902..1936
                     /locus_tag="PKX59_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOZ90819.1"
                     /translation="MIYKFSLIEKILLSNDIIPHPFADASFSVGLSLALGSAIKLKIA
                     DQLSTDFKEVRQIAKEASVSEVGAELTLDCLDALGYVQKKGSQYAFTKRGFKNLSTQS
                     PDNFRYFILFCDNLYKGYLSLDETIRLGKSAKADLLSMTDYDWELFSRAMIDISKTNY
                     KEVVGKIPVSKSATKLLDLGGLHGLYSIELCKRNPSLKATIVDLPPVKKYADECIAKY
                     NASGNVSFLPADFMKDDIPMGVDVILAFNIIHGFNPSENEILAKKVFDSLNAGGIFII
                     LDQIKGIGGNSQMSKATTSYMALNLLHEAGGKTYSKSEIDIYTSKVGFRKTELKKLHA
                     PGFGLIICYK"
     gene            complement(2044..3435)
                     /locus_tag="PKX59_07865"
     CDS             complement(2044..3435)
                     /locus_tag="PKX59_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOZ90820.1"
                     /translation="MSTSRREFLRTTSIAGFGTLLIPNSLFALSTLAFKGKKVKIGLI
                     GVGLRGQSNLEILAKRDDVEIIAFADPQSIMLSAAQKILSKNNKPTAKEFSNGEYDYR
                     NLLNDTGIDAVLISTPWEWHKVQGVEALYAKKIVGMEVGGALNLNECWEYVKTHEATG
                     TPLFAMENVCYRRDVMAILNMVRKGIFGELLHGEGGYKHDLRGVLFNDGITPYNSGVE
                     FGTKGYSEARWRTEHYIKRNGELYPTHGLGPVAMMMDINRGNRLLRLSSFASKARGLH
                     DYIVNHPKGGENHPNAKIVFKEGDVVTTQIACENGETILLTHDTSLQRPYNLGFKVQG
                     TKGLWEDFGWGDFDQGHIYFESLMNHSHKWDNSEKWLKEYDHPLWKRYEHLAEGAGHG
                     GMDWFLIHTFIECIKNNVEFPMDVYDLATWYSITPLSEKSIAQGGKVMNIPDFTKGKW
                     KTRKPVFGFSDEY"
     gene            complement(3560..5728)
                     /locus_tag="PKX59_07870"
     CDS             complement(3560..5728)
                     /locus_tag="PKX59_07870"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HOZ90821.1"
                     /translation="MKKVLLTLLAATTIISANAQRKAGINIKEQPTMTQRQCGTGELP
                     LEYETWMAAKMKEPNANKMQVVYNIPVVVHVIHNGTNVGVNQNISDAQINSQIAVLNE
                     DYRRQNPDTTSTPSVFGAVAADCEINFCLAQRDPSGNPTNGIDRINRNTQGWTAPPYT
                     KTYIDGTIKPATIWDPTQYLNIWVVPDYSASGNQLLGHATFPLNSTLTGLTGSYGTAT
                     NDGVVVWYKSFGRVGTLDALYNKGRTATHEIGHWLGLRHIWGDANCGNDYVSDTPTQQ
                     TANYGGQNPPYPCPSFPLVTCNNGPNGDMWMNYMDYCYDKCLNMFTAGQKSRMQIAMA
                     NGTYRAPLATSLGCSAPSAGPTAQFTANTTTIAPGGSVNFTDQSSNSPTSWSWSFPGG
                     TPSSSTAQNPSNIVYATAGTYNVSLTATNSNGSNTLTKNNYIVVTAGGTSTCDTISNF
                     DLVGDTATVYLAGTTPANGYVSGQNTYSDQAKADKYSNTTPNTTVDGALIYFGVGTSS
                     GTGQTASARVWDATGTGGMPGTAIGSVNVTYDQIAADAAGTLPTIIDFNPNVSIGVGN
                     YYVGVHFTYNAGDTLAIYTSRDGNTIPGTAYEQFSTGTWYAYSDATNSWDLNVAHAIF
                     PIVCTSTGIREVMTPSNNLVVFPNPSQGEFTLIVPQSDLKENVLVRVIDVKGAVVMHK
                     ELTPSGNGTYRLKLDAPAKGIYMLEVQTVNGLKKQRISVN"
     gene            5944..6600
                     /locus_tag="PKX59_07875"
     CDS             5944..6600
                     /locus_tag="PKX59_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOZ90822.1"
                     /translation="MFNQIGLIIYKEWKLEWRNRAAMAGLMVFILSGVYISYLSFKKI
                     VDVSAWNALFWLLILFMAVNALAKSFMAESRGLQLFYYSFLNPYAVILGKIIYNGLML
                     FLFGIITFFIYGILLGNPVQDIGMFILTVLLGCFGISSVLTMISAIASRAGHQSSLMA
                     ILGFPVLLPLLIVLIHLSKNAIDGIDFSVNLKYLTMLTALNLLVPSLAYILFPYLWRE
                     "
     gene            6637..7293
                     /gene="ccsA"
                     /locus_tag="PKX59_07880"
     CDS             6637..7293
                     /gene="ccsA"
                     /locus_tag="PKX59_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOZ90823.1"
                     /translation="MHNVWKIACVALLYFTLFAGFLMPVPELPILNESIRNLYFHVTM
                     WFAMIIHMVVTIVYSIRYLSGGKIQYDLKAEQYAITGMVFGIAGLITGMIWARFTWGA
                     FWVNDTKLNGAAAAMLMYAAYFILRQSTEDETKRAKLSAVYLVFAFPLMMAFIYILPR
                     MTDSLHPGNGGNPAFGQYDLDNQMRLVFYPAIIGWTLLGVWITSIRIRIKKLWNSHEN
                     "
     gene            7272..7499
                     /locus_tag="PKX59_07885"
     CDS             7272..7499
                     /locus_tag="PKX59_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HOZ90824.1"
                     /translation="MEFSRKLKILFSVLMLVATNVFAQTESALDETFRSNGKIYVVVA
                     VLAVVLTVLFIYLISIDRKLKKTEDKLKNKN"
     gene            7509..7892
                     /locus_tag="PKX59_07890"
     CDS             7509..7892
                     /locus_tag="PKX59_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HOZ90825.1"
                     /translation="MKKSYIIAMVLIAVAFAAIISTVSDSGTYANFTEASSHEGKVFH
                     VVGKLNRDKPYEYHPEKNANLFTFYLYDNNGNERKVLLNKAKPQDFDKSEQIVIIGKM
                     KGEEFIASDILLKCPSKYADEAQKS"
     gene            complement(7937..9997)
                     /locus_tag="PKX59_07895"
     CDS             complement(7937..9997)
                     /locus_tag="PKX59_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOZ90826.1"
                     /translation="MQQGLRIILTAIILFFGTGSNAQIFEGAKWSFRVEQTDSCEATL
                     ILKAVTVDKFHIYSQTINGADGPIPTSFTFNHSPEYTTVGKVKEGHAEEVYDQQFQMK
                     LKFFHGETEFKQKIKINTRKKFSITGFVTFMACDDEKCLPPTDVDFKFDLNDGKKKSC
                     ATTELAIPVNNTTPVVTDTAQAVMPPEHIDSATAAAQNDSVFTEVAQVSELEPGCGDG
                     STSTATDKSMLGIFIAGFLGGFLALLTPCVFPMIPMTVSFFTKRSGSRQKGLMNALIY
                     SISIIIIYVGLGFLVTVTLGSDALNDMASNVFFNMTFFIVFLIFAFSFFGAFEIVLPS
                     WLVNKADSASDRGGLIGIFFMAFTLTLVSFSCTGPIIGTLLVEAAHNGSYLGPIIGMT
                     GFALALALPFGLFSAFPGWLNSMPKSGGWLNTVKVTLGFIELALAFKFLSNVDLAYHW
                     EFLKREIFIAIWVIIFGMLGFYLLGKLKFSHDSDVKHITVPRLMFAIISLTFTLYLIP
                     GIWGAPLRMISGFPPPEFYKEWVTEKSSNHCPHNLNCYHDYDAGMAYAKTVGKPVMVD
                     FTGWSCVNCRKMEDNVWSDPEVLKRLSDKYVLISLYVDDKEQLPEDKQYVSATTGKKI
                     RTIGNKWSDLQTNVYKTNSQPYYVLLDHQGKILAEPRGYTPDIKTYTKYLDEGLCRFE
                     NRKK"
     gene            complement(10093..11931)
                     /locus_tag="PKX59_07900"
     CDS             complement(10093..11931)
                     /locus_tag="PKX59_07900"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ90827.1"
                     /translation="MFTTRLFLLLKKSAFLIMPVMLLSLFTSAQGLNNLDLARQYEST
                     GEFDKAAVYYEKQYNIDPFGTYEPYLKCLKQIKDYKEAEKLIKKQAKRGAGAGKYNVD
                     LGLLYELEGDNEKAKKQFETAIKNLHPDVGDIYAVANSFIMNGKPDLALETFLKGRSL
                     VPDNNFGFDIAELYGRKGETQKMIDEYLDIMTENTMFQANVQSVLQYKLSQDSDSNLA
                     NLLRLSLLRKIQREPSQLIYSEFLYWLFMQEKDFESAYIQAKALDKKTGDTGNRIYSL
                     GEICISNQAWTVAEKCFDYIVAKGSNHPLYIRAKIAMLSAAFEKTTDSHYTQTELLAL
                     EKQFTEALQELGKNSITAPLMVKHAQLEAFYLDKVAEGRKILEEVIAMPALSPNYIAE
                     VKLELGDIMILDSDLWEASLTYSQVDKDFKHDAIGREAKFRNARLSYYLGEFEWAEAQ
                     LSVLKQATSQLISNDALDLALLISDNIGDDSITEPLQIYSRAELLAFQHKNDLALQTL
                     DSILVLYPGRKITANVWFKQAGIYLTKGDFEKAITLYSDIVKNYADGVLADDALFRIG
                     GIYENNLKDTEKAKSTYEQFIDKYPSSVFIAEARRHYRLLRGDKIN"
     gene            complement(11934..>12193)
                     /locus_tag="PKX59_07905"
     CDS             complement(11934..>12193)
                     /locus_tag="PKX59_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOZ90828.1"
                     /translation="LEVFSAAQEKWLEVSSVSNFESFQSNRLKLRFKGNDGKTQLTHT
                     LNGSALALPRIVAALLENNYNGQYIHIPKAIQHYTGFDRID"
BASE COUNT         3628 a         2291 c         2191 g         4083 t
ORIGIN      
        1 aaattaaagt gacaaagtcc agtaatattt atattccaaa caatgaagtc gaaaagacat
       61 tcataaaatg tatttggcga ctttcagaat atgatattca ggaaagaaaa gaaattatcc
      121 ttcccaaagg tacagttgaa attattttta atttttctga taaaatcaac tacttcagtc
      181 cttcatataa aattgaaaaa tcactacctg atgtctttgt aaatggaatt aattttaagc
      241 cattcgaact tactaaaacc aaacaacaaa aatttctggg agtacaattg aacagtatcg
      301 ggctgcgatt actttttaaa attccgtctt ttgagttgaa taacattgtt tgcgatggaa
      361 atgatatttg taaacaactc agtaaccttg ctaatgattt aaatggagag gaggaattta
      421 gtcaacaggt taaaattatt ttatcgtgga ttagaaacac agtttctttt aatcaaatta
      481 agtatgcaac aagccgtgct gaaaagttaa caagttgtat ttgttataat aatctcacag
      541 taaaaaagtt aagtaaagaa ataggtttgt cagaacgcca actaagcagg ttctcgaatg
      601 attggttggg gatgagcaca gaacaattta ttcattacag aaagtatctt aattgcttaa
      661 atctgcttca ccaatcaaaa caaaacctca cagcattggg catgcaggca gggtattacg
      721 accaatcaca ttttattcat cagtttaaat catttactga catgacgccc cgccaataca
      781 gagaagcaaa gacagatttg cctggacata tttattaagc acatggaatg tccgaattgt
      841 acaattttta tttttatttc tttttgaatt ttgctgttaa ctttttctaa acagctaaat
      901 catgatttat aaattctcac tcattgaaaa aatacttttg tcaaacgaca tcattccgca
      961 tccctttgct gatgcatcct tttccgtagg gctgtctctg gcgttaggaa gtgctataaa
     1021 gctgaaaatt gcagatcaac tctctacaga ctttaaagaa gtgagacaaa ttgcaaaaga
     1081 agcaagtgtg agtgaggtag gagcagagtt gactcttgat tgtttagatg ctttaggata
     1141 tgttcagaag aagggttctc agtatgcttt cacaaaaaga gggtttaaga atttgtcaac
     1201 gcaatctcct gataatttca ggtattttat tttgttctgt gataatttgt ataaaggtta
     1261 tttaagctta gacgaaacta ttcgattggg taaaagtgct aaggctgatt tattgagtat
     1321 gacagattat gattgggagt tgttttcgag agcaatgatt gatatttcta aaacgaatta
     1381 taaagaagta gtgggcaaaa ttccggtttc aaagtctgca acaaaactcc ttgacctggg
     1441 cggcttgcat ggcttataca gtattgaact ctgtaaacga aatccaagtc ttaaagcaac
     1501 tattgttgat ttgcctccgg taaagaaata tgctgacgaa tgtattgcca agtataatgc
     1561 ttcaggaaat gtttcttttc ttcctgcaga ttttatgaaa gacgatattc caatgggtgt
     1621 tgatgttatt ctcgcattta atattattca cggtttcaat ccttcagaga atgaaatatt
     1681 agcaaagaag gtatttgatt cactgaatgc aggaggcatt tttattattc ttgatcagat
     1741 taaaggtatt ggaggaaact ctcagatgtc gaaggcaacc acttcttata tggctttaaa
     1801 tttactacat gaggcaggag gaaaaactta ttccaagtca gaaattgata tttacacaag
     1861 taaagttggt ttcaggaaga cagaattaaa aaaactacat gctccgggtt ttggattgat
     1921 aatttgctat aaataaggag atttcttaag gtaagttatt ttacagaagg ctacttcgta
     1981 aatggtaata ccgttagttg aaaagataca atttgcgcta ctttgattgg gttttaaaat
     2041 aaattaatat tcatcactaa agccaaacac aggctttctg gttttccatt tgcctttggt
     2101 aaagtcagga atgttcatca ccttgcctcc ttgcgcaatt gatttttcac ttaatggtgt
     2161 aatagaatac catgttgcca agtcatacac atccattgga aattccacat tatttttaat
     2221 gcattcaatg aatgtatgta tcagaaacca atccatgcct ccatgccctg cgccttcggc
     2281 aagatgttcg tatcgtttcc ataaaggatg atcatattct tttaaccatt tttctgaatt
     2341 gtcccatttg tgcgaatggt tcattaagct ctcaaagtaa atatgcccct ggtcaaaatc
     2401 accccatcca aaatcttccc aaagtccttt tgttccctga actttaaaac ctaaattata
     2461 aggtcgttga agactggtgt catgtgtcaa taaaattgtt tcaccatttt cgcaggcaat
     2521 ttgtgttgtt accacatcac cctctttaaa tacaatcttt gcattaggat ggttttctcc
     2581 accctttgga tgatttacaa tataatcatg taagcctctt gctttagatg caaaagatga
     2641 aagccgcagt aatctattac cacggttaat gtccatcatc attgctacag ggcctaagcc
     2701 atgtgtgggg tataactctc catttctttt tatgtaatgt tctgtcctcc accttgcttc
     2761 actgtaacct ttagttccga attctactcc tgaattgtaa ggtgtaatac catcattaaa
     2821 caacacgcct ctcaagtcat gtttatagcc accttcgcca tgaagcaatt ctccaaaaat
     2881 tccttttctg accatattta aaatagccat cacatctctg cgataacaaa cattttccat
     2941 agcaaacaaa ggtgtgcctg ttgcctcatg tgtttttaca tattcccaac attcattcag
     3001 gtttagggca ccgccaactt ccattcctac tattttcttg gcatacaagg cttctacacc
     3061 ttgcacttta tgccactccc acggagtaga aattaaaaca gcatcaatac cggtatcatt
     3121 caacagattg cggtaatcat attctccatt tgaaaattcc tttgcagttg gcttgttatt
     3181 tttcgataat attttttgtg ctgcggaaag catgatggat tgcggatcgg caaatgcaat
     3241 aatttctaca tcatctcttt ttgccagaat ttctaaatta ctctgacctc tgaggccaac
     3301 acctattaag cctattttta cttttttgcc tttaaatgca agtgtagata aggcaaataa
     3361 agaattagga attagtaatg tgccaaatcc ggctattgaa gttgtgcgta aaaattctcg
     3421 tctggaggtg ctcattggat tttattttta ttactcacaa acttaataaa aacaactttt
     3481 aaactcaatg ttcaaaatca agcagcaata aaaaaagctg ctcttgtgaa gcagcttttt
     3541 ttatcattac ttatgttttt taattcacag atatacgttg ttttttcaaa ccgtttactg
     3601 tctgcacctc aagcatgtaa atgcctttag ccggagcatc aagttttaaa cgataggttc
     3661 cgtttccgga tggtgttaat tctttgtgca tcacaacagc acctttaaca tcaattacac
     3721 gtaccaaaac attttctttc aggtcgcttt gcggtacaat caatgtaaat tctccttgtg
     3781 atggatttgg gaacaccact aaattgttgc tcggtgtcat aacttctctt attcctgttg
     3841 aagtacatac tatcggaaat attgcatgtg ctacatttaa atcccatgag ttagtggcgt
     3901 cagaataagc ataccaagtg cctgtactaa attgttcata agctgtgccc ggaatggtat
     3961 ttccatctct gctggtgtaa atagctaagg tatcacctgc attatatgtg aaatgtacac
     4021 caacgtaata attaccaacg ccaatactta cattcggatt aaaatcaata atagttggta
     4081 aagtacctgc tgcatctgca gcaatctgat cgtaagtaac atttacgctt ccaattgcag
     4141 ttccgggcat accaccggtt ccggttgcat cccaaactcg agcacttgcc gtttgtcctg
     4201 tacctgatga tgtaccaaca ccaaagtaaa tcaacgcacc atctacagtt gtattaggtg
     4261 ttgtgttgga gtatttgtct gcttttgctt gatctgaata ggtgttttgt cctgaaacat
     4321 atccatttgc tggtgtagtg ccggctaaat aaacagtagc tgtatcgcct actaagtcaa
     4381 aatttgaaat agtgtcgcat gtagaagtac cacctgctgt aactacaatg tagttgttct
     4441 ttgtaagtgt attgcttcca tttgaattag ttgcagttaa agaaacatta taagttcctg
     4501 cagtcgcata aactatatta cttggatttt gtgctgttga ggatgatgga gttccacctg
     4561 ggaatgacca tgaccacgat gttggtgaat tgctgctttg atcagtaaag tttacactgc
     4621 caccgggagc aatagttgtt gtatttgcag taaattgtgc tgttggtcca gctgaaggtg
     4681 cgctgcaacc tagtgatgtt gctaagggtg cacggtatgt tccgttagcc attgcaattt
     4741 gcattcttga tttttgtcct gcagtaaaca tattcagaca tttgtcataa cagtagtcca
     4801 tatagttcat ccacatatct ccatttggac cattattaca tgttactaat ggaaaagaag
     4861 gacatggata aggcggattt tgtccaccat aatttgcagt ttgttgtgtt ggcgtgtcag
     4921 atacatagtc atttccacaa ttagcatcgc cccaaatatg acgcagacct aaccagtgac
     4981 ctatttcatg cgttgctgta cggcctttat tgtataaagc atctaaggta ccaacacgac
     5041 caaaagattt ataccaaact actacaccat cattggtggc cgtaccgtaa cttcctgtca
     5101 aacctgttaa tgtactgttt aaaggaaatg tagcatggcc aagcaattga ttccctgaag
     5161 cggaataatc aggaacaacc caaatattta agtattgtgt aggatcccaa atggttgccg
     5221 gttttatggt gccgtcaata taggtttttg tatatggagg tgcagtccag ccttgtgtat
     5281 ttctgttaat acgatcaatt ccatttgtag gattaccact tggatcacgt tgtgccaaac
     5341 agaagttgat ttcgcaatct gcagctaccg caccaaacac agaaggagtt gatgtggtgt
     5401 caggattttg tctgcggtaa tcttcattca aaactgcaat ctgagaatta atttgtgcat
     5461 cgctgatgtt ttggtttaca cccacattgg ttccattatg aattacgtga acaacaacag
     5521 gaatgttgta aaccacctgc attttatttg cattcggctc tttcatttta gcggccatcc
     5581 atgtttcata ttccaatgga agttcaccgg ttccgcattg acgttgtgtc attgtcggct
     5641 gctctttgat attgatgccg gcttttcttt gtgcattagc cgaaataatt gttgttgcgg
     5701 ctaataatgt tagtaaaact tttttcatta ataatgattt tataattttc aaataaaaat
     5761 tgagacggta aatctaaaaa aaaatcaaat tcgtgcagca aattcaaaaa caaaatttga
     5821 atttatttcg aattatcttt tcaataaatt tttttcatca ttttgttgtt tactactgac
     5881 tttcaattaa acatttatgg tcgttttctt cattttaagg cttgtttttc tcaactttgt
     5941 gccatgttta atcagatagg tttaataatt tataaagaat ggaaattgga gtggcgcaac
     6001 cgagccgcta tggccggtct tatggttttt attctatcag gtgtttatat cagttatctc
     6061 agttttaaga aaattgtaga tgtttcggct tggaatgctt tgttctggct gcttattttg
     6121 tttatggctg taaatgcact tgcaaaaagc ttcatggcag aatcgcgtgg tcttcagtta
     6181 ttctattatt catttctgaa tccttatgcc gtaattttag gaaagataat ttataatggt
     6241 ttgatgttgt ttcttttcgg catcatcact ttttttatct atggaatttt actgggtaat
     6301 cctgtgcagg acataggcat gtttatcctt actgttttat tagggtgctt tggaatttca
     6361 tctgtgctga ctatgatttc cgccattgca tcccgtgcag ggcatcagtc aagcctcatg
     6421 gcaattttag gttttcctgt tttattgcct ttgctgattg ttttaattca tctttctaaa
     6481 aatgctattg acggtattga tttttcagtg aatttaaaat acctaaccat gctaacagct
     6541 ttgaatttac tcgtaccttc tttagcctac attttatttc cctacctttg gcgcgaataa
     6601 ttatcttcat gttgacatta aagttctttt ttcatcatgc ataatgtctg gaaaattgct
     6661 tgtgtagcat tgctctattt tacacttttt gcaggcttcc ttatgcctgt acctgagttg
     6721 cctatcctta atgagtctat cagaaattta tatttccatg taactatgtg gtttgccatg
     6781 attattcaca tggtagtaac aattgtttac agcatacgct atttatcagg aggaaagatt
     6841 cagtatgatc taaaagccga acagtatgcc attacaggaa tggtgtttgg tattgcaggt
     6901 ttaattaccg gaatgatttg ggcacgtttc acttggggtg ctttttgggt taacgatacc
     6961 aaactcaatg gtgctgctgc tgccatgctc atgtatgcag cttattttat tctcagacaa
     7021 tcaacagaag acgaaaccaa acgtgccaaa ctcagtgcag tatatttagt ttttgcattc
     7081 ccactgatga tggctttcat atacatcctg ccacgcatga cagattcact tcatcccggc
     7141 aatggtggca accctgcttt tggacaatat gatcttgaca atcagatgcg actggttttt
     7201 tatcctgcaa ttatcggatg gacattgctt ggcgtatgga taacatctat tcgaataaga
     7261 attaaaaaat tatggaattc tcacgaaaac taaaaatact tttttctgtt ttaatgttgg
     7321 ttgcaacaaa tgtgtttgca caaacagaaa gtgcacttga cgaaactttc cgcagcaatg
     7381 gaaagattta tgtggtagtt gcagtgcttg ctgtggtgtt aacagtattg tttatttact
     7441 taatcagtat tgacaggaaa ttgaaaaaga cagaagataa attgaagaat aaaaattaaa
     7501 aaacagacat gaaaaaatct tatatcatag caatggtgct cattgcagtt gcatttgctg
     7561 ctattatcag cacagtgagc gactccggca cctatgccaa tttcactgag gcctcatcac
     7621 atgaaggtaa ggtatttcat gtggttggta aacttaaccg cgataagcct tatgaatacc
     7681 atcctgaaaa gaatgccaat ctttttactt tttatcttta tgataacaat ggcaatgaaa
     7741 gaaaagtgtt gctcaacaaa gccaagccgc aagactttga taagtcagag cagattgtaa
     7801 ttattggtaa aatgaaaggc gaagagttta ttgcttctga tattttgttg aagtgtccaa
     7861 gcaagtacgc tgacgaagca caaaagagtt aagttgtttt tagcagaaca taactttaga
     7921 taagcaacaa tcttatttat tttttccgat tttcgaaacg gcataaacct tcatcaagat
     7981 atttagtgta ggtttttatg tcaggtgtat aaccacgtgg ttcagcaaga atttttccct
     8041 gatgatctaa taaaacatag taaggctgcg agttagtttt gtaaacattg gtttgtaaat
     8101 cgctccattt attaccgata gttcttattt ttttacctgt tgttgcagaa acatattgtt
     8161 tgtcttccgg tagttgctcc ttgtcatcaa cataaagtga aatgagcaca tatttatccg
     8221 aaagcctttt cagcacttca ggatcactcc acacattgtc ttccatttta cggcagttaa
     8281 cacaactcca tcctgtaaaa tcaaccatca ccggcttgcc tacagtttta gcgtatgcca
     8341 tacctgcatc gtaatcatga tagcagttta agttatgtgg acaatgattg cttgattttt
     8401 ctgttaccca ttctttataa aattcaggtg gaggaaagcc gctgatcatt cttaatggtg
     8461 cgccccatat tcccggaatc agatacaatg taaatgttaa tgatatgatg gcaaacatca
     8521 gtcggggtac agtgatatgt tttacatcgc tgtcatgcga aaattttaat ttgcctaata
     8581 aataaaagcc aagcatacca aaaatgatta cccagatggc aataaaaatt tcacgcttca
     8641 aaaattccca gtgataagcg aggtcaacat tcgataaaaa tttaaatgcc aatgccaatt
     8701 caataaaacc gagtgtaact tttacagtat taagccatcc acctgattta ggcatggagt
     8761 ttaaccaccc cggaaatgca gagaaaagtc caaatggcaa tgcaagtgcc aaagcaaatc
     8821 ctgtcattcc tataattgga cctaaataac ttccattgtg tgcagcttct actaacagtg
     8881 tgccgataat tggtcctgta catgagaatg aaaccaatgt caaagtaaat gccataaaga
     8941 aaattcctat caatccgcca cggtcggagg cgctgtcggc tttgttaact aaccagcttg
     9001 gtaatacaat ttcaaatgca ccaaagaatg aaaaagcaaa aatcaggaac acaataaaaa
     9061 atgtcatatt aaaaaagaca ttgcttgcca tatcgttgag tgcatcacta cctaatgtaa
     9121 ccgtgactaa aaatccaaga cctacataaa taataatgat agaaatagag tagattaatg
     9181 cattcatcaa ccccttctga cgtgaaccac tgcgctttgt aaaaaaactt actgtcatag
     9241 gtatcatcgg aaaaacgcaa ggtgtaagta aagctaaaaa gccacctaaa aatcctgcaa
     9301 taaaaattcc gagcatgctt ttatctgtgg cagttgatgt tgagccatca ccgcagcccg
     9361 gttcaagttc tgatacttgt gctacttctg taaatacact gtcgttttgt gctgctgccg
     9421 ttgctgaatc aatatgttcc ggtggcatca ctgcctgtgc agtgtctgta accacaggtg
     9481 ttgtattgtt tacaggaatt gcaagttcag ttgtagcaca cgattttttc ttgccatcat
     9541 tcaaatcaaa tttaaagtca acatctgttg gtggcagaca tttttcatca tcacaagcca
     9601 taaaggttac aaaacctgta atgctgaatt tttttcttgt gttgattttt attttctgct
     9661 taaactctgt ttcaccatga aaaaatttca gtttcatctg aaactgctga tcatatactt
     9721 cttcggcatg accttctttt actttgccaa ctgtagtgta ttcaggagaa tgattaaaag
     9781 taaaacttgt gggtataggc ccatcagcac cattgatggt ttgagaatag atatgaaatt
     9841 tatctaccgt tacagctttt aatatcagcg tagcttcaca actgtctgtc tgttcaactc
     9901 tgaaactcca cttggcacct tcaaaaatct gcgcattact gcctgtgccg aaaaataata
     9961 tgatggcagt taaaataatt ctcaaacctt gttgcatagt caaatttaac ttcaccggat
    10021 taaagcccgg ttttaaatgt ggcacaaaat aacaaaatca aagcattgtg gaatgtaagt
    10081 tttgtaacaa agtcagttta ttttgtcacc tctgagtaat ctgtaatgcc ttcgtgcttc
    10141 agcaatgaat acgcttgaag gatatttgtc tataaactgc tcataagtgc tttttgcctt
    10201 ctcagtgtcc ttcagattgt tttcataaat gccgccaatt ctgaacaaag catcatcggc
    10261 caatactcca tcagcataat tttttacaat gtcgctataa agcgtaatgg ctttttcaaa
    10321 atctcctttt gtcaaataaa ttccagcttg tttaaaccat acgttggcag taatttttct
    10381 tcccggataa agcacgagaa tgctgtctaa tgtttgcaat gcaagatcat ttttatgctg
    10441 aaatgcaagt aattctgcac gtgaataaat ctgcagaggc tctgttatag agtcgtcacc
    10501 aatgttatca gaaattagca gtgcaaggtc aagtgcatcg ttcgaaatca gttgcgaagt
    10561 agcttgtttc aacacactta attgtgcttc tgcccattca aattctccca gataataaga
    10621 tagtcgtgca ttgcggaatt tagcttcgcg accaatggca tcgtgtttaa aatctttgtc
    10681 aacctgcgag taggtgagcg atgcttccca taagtcgcta tctaaaatca ttatgtcgcc
    10741 caattctaat tttacctctg caatatagtt tggcgatagt gcaggcatgg caattacctc
    10801 ttcaagaatt ttacgtcctt cggcaacttt atcaagataa aaagcttcta attgtgcatg
    10861 tttaaccatc agtggtgcag taattgaatt tttacccaac tcctgcagtg cttccgtaaa
    10921 ttgtttttct aatgccaaca gttctgtttg cgtgtaatga ctatctgttg ttttttcaaa
    10981 ggctgcacta agcatggcaa tttttgctct gatataaagc ggatgattgc ttccttttgc
    11041 aactatgtaa tcaaaacatt tttctgcaac agtccatgcc tggttcgaaa tacatatttc
    11101 gcctaaagaa taaattctgt tgcccgtgtc accggttttt ttatccaaag ctttggcttg
    11161 aatataagcc gattcaaaat ctttttcctg cataaagagc caatacaaaa actcactgta
    11221 aatgagttgt gatggctcac gttgaatttt gcgaagtaat gacagccgca acagattagc
    11281 aagatttgaa tcactatcct gacttagttt gtattgcaat acggattgta cattagcctg
    11341 aaacatggtg ttttcagtca tgatgtctaa gtattcatca atcattttct gtgtttcgcc
    11401 tttgcggcca tacaactctg caatatcaaa accaaagttg ttgtcaggca ctaaacttct
    11461 tcctttcaga aaagtttcca atgctaagtc aggtttgcca ttcatgataa aactgttggc
    11521 aactgcgtaa atgtcaccta cgtcagggtg aaggtttttt atggctgttt caaattgctt
    11581 ttttgctttt tcattgtcgc cttccaattc atacaacaag cccaagtcaa cattgtattt
    11641 tcctgcacct gcaccacgtt tggcctgttt tttaataagc ttttctgctt ctttatagtc
    11701 tttaatttgt ttcagacatt tcagataagg ctcataagtg ccaaaggggt caatgttgta
    11761 ttgcttttca taatacactg ctgctttatc aaactcaccg gttgattcat attgacgtgc
    11821 cagatcaaga ttatttaatc cctgtgctga agtaaacaaa ctcagcaaca ttacaggcat
    11881 tattaaaaat gctgattttt ttaaaagcag aaataatctt gttgtaaaca tgtttaatct
    11941 atgcggtcaa atcctgtgta gtgctgtatg gctttgggta tatgaatgta ctgtccgtta
    12001 taattgttct ctaaaagtgc ggctacaatt cttggcaatg ccaatgcact tccgtttaat
    12061 gtatgtgtca attgtgtttt accatcattg cctttaaatc gtaattttag tctgttactc
    12121 tgaaaacttt caaaattaga aaccgaactc acttccagcc atttttcctg agcagcagaa
    12181 aacacttcca ggt
//
LOCUS       DAOOOI010000060        11554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1083_length_11554_cov_13.923646, whole genome shotgun
            sequence.
ACCESSION   DAOOOI010000060 DAOOOI010000000
VERSION     DAOOOI010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11554
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1083_length_11554_cov_13.923646"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..983
                     /locus_tag="PKX59_07910"
     CDS             216..983
                     /locus_tag="PKX59_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOZ90829.1"
                     /translation="MQSKLWGQRPKDWATIQEPTGKIGYEHALEFIKLQPIDKLLDIG
                     CGTGYFISLAAQSGAEIIGFDATGQFIEEAKQHNPSVKFLVGEMEELPFADDSFDIVT
                     GFNSFQYASDVKNALMQAKRVLKKEGKIVVMVWGNKEDCDIISFLKAIGSLMPPPPSG
                     SPGPFALTENHLLERILEDIGFKILNVKDVDSIWDYPDTETAIKGLLSAGPSAKAIEH
                     AGFENVVKSTLGVIKSYSKSNGHIVFANKFRIVIAEK"
     gene            complement(1030..1536)
                     /locus_tag="PKX59_07915"
     CDS             complement(1030..1536)
                     /locus_tag="PKX59_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOZ90830.1"
                     /translation="MNKLLHQFFTNDHHRLEKLLDKAITTPEIDMTFYMPFRSGLLRH
                     IKMEENILFPAAKAIDANKIQEILPQFRLEHGALTALMVPPPTPDLIKVIKHILEKHD
                     FEEEKPGGLYDVCELLTQSQTQELLNQLEKTPEVPLHPPNPTPFAFDAAKRALVRAGY
                     DYDEIIMM"
     gene            1642..3654
                     /gene="ligA"
                     /locus_tag="PKX59_07920"
     CDS             1642..3654
                     /gene="ligA"
                     /locus_tag="PKX59_07920"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOZ90831.1"
                     /translation="MSKISEKEAGEKIKQLTVDLNHHNYLYYVKAQPVISDYEFDVML
                     QELIALEKQFPNLLTTESPSQRVGGFISKEFVTVTHSYPMMSLGNTYSEEELRDFDER
                     VRKVIGSDFEYVCELKFDGVAIGLTYQNGKLLRAVTRGDGVRGDDVTTNVRTIRSIPL
                     SLMGNNFPDKFEIRGEIILDKKTFERINAERADIGETPFANPRNSAAGTLKLQDSSEV
                     ARRKLDCFLYALYGDFDFSTHYDGLKMAADWGFKISEHYQLCKSIEDVLTFIESWRKK
                     RDLLPFDIDGIVIKVNNFRQQEELGFTAKSPKWAIAYKYKAESASTILNSVVYQVGRT
                     GTITPVANLKPVLLAGTTVKRASLHNADQIQKLDLHMHDTVFVEKGGEIIPKITGVDF
                     SMRQAGAKPVSYITHCPECGTELIRLEGEANHYCPNEEGCPPQIKGRLVHFTSRKAMN
                     IDGLGVETIDQLVEAELLKNVADIYHLKSEKLLQLERMGEKTVSNLFEAIEKSKQVPF
                     ERVLFAIGIRFVGDTTAKKLARHFGSYQKISQATLEELIQTPEIGDKIATSIFDFVRE
                     LENIKMVEQLQSAGLQFKLDKAAMQEAGSKLKGLSFVITGVFQKFERDKLKETIEING
                     GKVSGSLSSKTSYLLAGSDCGPAKLEKAEKLKINIISEDDFLKMLQ"
     gene            3651..4205
                     /locus_tag="PKX59_07925"
     CDS             3651..4205
                     /locus_tag="PKX59_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90832.1"
                     /translation="MKIGKAIKQKAASYLFYRELQRQHHQPRTTSFSNARTIGILYDA
                     TIERNYEIVKAYVKHLRDDFKKDVLALGYYDGNELPPMRFSKLGLDFFTKKNLNWHFK
                     PSHPMISKFVNLDFDYLICLNVDRCMPLRYISAATKAGFKIGRYEKGYAGLYDFMISV
                     NGQVTLPQFIDHVNNYLKHIGHDK"
     gene            4202..5077
                     /gene="dapA"
                     /locus_tag="PKX59_07930"
     CDS             4202..5077
                     /gene="dapA"
                     /locus_tag="PKX59_07930"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOZ90833.1"
                     /translation="MNKFTGTGVALVTPFLKNGKIDFNALRKVVNHVIKGGVEYLVVM
                     GTTGESVTLSKEEKKDILKFISEVNNKRTHIVFGVGGNNTLETARELEQIDARMADAI
                     LSVSPYYNKPNQSGIVQHYKTLSGVSQLPIILYNVPGRTGSNLLPQTTLEIAASCKNI
                     IGIKEASGNMEQIMYLIQHRNKGFLVISGDDAITMPLIAAGADGVISVIANAFPKQFS
                     TMVRLALQGKITESRKLHYQLLDLIPLLFAEGSPSGIKYVLSQLDICQNYFRLPVASI
                     SKSLAQKINTVIKGI"
     gene            5104..6117
                     /locus_tag="PKX59_07935"
     CDS             5104..6117
                     /locus_tag="PKX59_07935"
                     /inference="COORDINATES: protein motif:HMM:NF015940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HOZ90834.1"
                     /translation="MGHPAHFHLFKNTIIALQQSGHEAMILIKKKDILEDLLRHAGMQ
                     YININPEGRRDDKFSIALKLLKRDFQFLKICLRFKPQIMIGTSAEIAHVGKLLGIRSI
                     VVNEDDAEVVPWFAKLAYPWATNILAPYCCSVGKWEHKKIGYKGYHELAYLHPSNFSP
                     KKENIKNLGDEPFFILRFAKLTAHHDEGRKGINTLIAEKLINMLSKHGRVYITSEREL
                     ESQFEQYRIAINPLHIHDALYFAQMYIGDSQTMAAEAAVLGTPAIRFNDFVGQISYLE
                     ELEKEFALTFGFKTSDADAMLVHVETLLNQQNLKLQWQEKRSMMLNQCIDLNQFMIKL
                     LTQ"
     gene            6257..7369
                     /locus_tag="PKX59_07940"
     CDS             6257..7369
                     /locus_tag="PKX59_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOZ90835.1"
                     /translation="MIFKTDNTEAKLLQLEEFAELDAVLATKAKNDVRNERFVFNLIE
                     KYVDTSRSDCFTFAANNHDDANQLADETYHAVIHFKRVNNFQFINKQFEAINTKLPKG
                     GIFVSKVETNDLRKQRLMEKYPPYLNKVYYFFDFVFKRVFPKLPVTQQIYYAITKGHN
                     RVLSKTEMLGRLYSCGFKVIEERYIGNRLYIVSEKFRDPAYDEDPSYGVIYPMKRVGK
                     GGKIISVYKFRTMHAYAEYLQEYVYEKNNLEEGGKFKDDFRVSRVGRMLRKYWIDELP
                     MILNVLKGDLKIVGVRPLSRHYLSLYSKELQDMRKKFKPGLIPPYYVDLPKTMEEIMD
                     SEMRYLKAYQKSPFLTDVRYFLMVFYTILFKRARSK"
     gene            7429..9072
                     /locus_tag="PKX59_07945"
     CDS             7429..9072
                     /locus_tag="PKX59_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90836.1"
                     /translation="MKASRLVFILILSFAFQLNISAQYVPQSVFNKPLYDFMDDLANL
                     HIITLNDAIKPYSRKQIAEALQTAESQKEKLNATQQKQLAFYLKDFNKELFTGKGFKK
                     RLDLFYFKDSLFNITVNPVGGFSYYKNENDNMYRQYAGGEFWATLGKGLSIFGSYRDY
                     TDSRAVADDKYLNDLPGYNYKYKGAVGSPKRGGSFDETRGGIAYAWKWGHVGIVKDNF
                     EWGSNNHGANIISTKAPSFARLELKLNPKKWFEFNYFHGWLYSEIKDDSAAYFSGNPP
                     NHRQVYFSKYFAANMFTVKPFKQFDFSFGNSIVYSDQLEVAMFIPFMFFKSLDHSKTG
                     QGSNYSGQNSQLFFNISSHNIKYTHLYISLFVDEIAFGRMMDKNEHSNFYSLKAGAAV
                     MLPFYTSATFIAEYTRTNPVTYRHFVNTTTYESNRFNMGHYLRDNAQEIYLGARCAPI
                     SGLYISAGYTSAAVGPIYPYTGKDKSGLGLPFLKTKEYEFTSLDLNIKYELFNDIFLS
                     AGVKIDDNKGAMANVYTAPFYYGKNGKTTTLFGGFNIGF"
     gene            9075..9599
                     /locus_tag="PKX59_07950"
     CDS             9075..9599
                     /locus_tag="PKX59_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90837.1"
                     /translation="MNFDNLTTALLIIAPTFIVAGGMYMLIKNMLERDYRLKLLDARR
                     LIQKDMLPLKLQAVERMVVFLERIQPENILFRILQPGMNVRDLQVDLLAAIRQEYEHN
                     VSQQVYVSVQSWSLITKAKDDIIATVNLAADSLDKTEKAIVLSQIILQRSSAGNNFAG
                     AAILSLKSEVDSLL"
     gene            9633..10634
                     /locus_tag="PKX59_07955"
     CDS             9633..10634
                     /locus_tag="PKX59_07955"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOZ90838.1"
                     /translation="MSFSTQIIFKTAVKLQLQNLLKLIPASPNGVTILCFHRISSQYD
                     YFWQPIYPETFRLMLESLVKEYQIIPISQIENKSFRSTKPPLVLSFDDGYKDFIEEAI
                     PVLHYYEAPSNHNIVIDCADGKSYIWTQKLNHLFNLLRNSSRFSINIALDKNFVLDIK
                     QPCKNWFAHYYKTLFYLFKLDVIDRSECIAVLEQYLNITETDYPIKMMNWDDIKFIAT
                     NNVEIGSHTASHVSLSAVKNDVTFEHELNYSKKRIEEMLKCECPVIALPNGLSNGEAN
                     QKISEAGYKTVLCLAGSGSNAGTSTDKIRFYNRLNMIQEPNSYMNVRIKLYEKGFIK"
     gene            10631..>11554
                     /locus_tag="PKX59_07960"
     CDS             10631..>11554
                     /locus_tag="PKX59_07960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ90839.1"
                     /translation="MSEVQLRQLLKDEIGLLPGLMENCFSMKVDKAYFKWKYLDNPAG
                     NFIGFGAFFENKLVGFYGVIPERYQINNSEVIIFQSGDTMTHSTQRRKGLFEKLATLC
                     YNYVREHHQLAVIGFGGDKSTPGLVKMGWCHIADVKYFFIHALHCKWINNFTTEDRNF
                     NIVEKTDIHVILDIQKNFGFKKNIKQHYSKEFLKWRLANPRFNYRCFICYQGQNPVGY
                     VICYEDEGKLFLLDYCSISVKVQKTLIRRLSQICIKSKLKGIITIAIPKSEEGKWLKK
                     AGFLRNPFKKGPLTTNIPFMILTDQKPESQLC"
BASE COUNT         3863 a         1835 c         2210 g         3646 t
ORIGIN      
        1 atgtaagaag aattgcattt cgaatctctt cttttagaaa ttatatttcc taatgaatca
       61 ataaaattat attttcaatt tttatcgatt cattaggaaa aaatatgtag tcaagagtag
      121 tttagtagaa tgtattttca ttattttagg atagtcattg ttgatattgt tatttaaaat
      181 taatttttaa taataaggtt atgggatcgc aacaaatgca gagtaaactt tggggacaaa
      241 gacctaaaga ttgggcaaca attcaggaac ctactggaaa aataggatat gaacatgccc
      301 ttgaatttat taaattacaa cccatagata agttattaga tattggctgt ggtacaggat
      361 actttatcag tttagctgca caatccggag ctgaaataat aggctttgat gcaacaggac
      421 aatttattga ggaagcaaaa caacataatc catcagtgaa atttcttgtt ggtgaaatgg
      481 aagaacttcc ctttgcagat gattcatttg atattgttac tggctttaat tcttttcaat
      541 atgcttcgga cgttaaaaat gctttaatgc aagctaaacg agtattaaag aaagagggaa
      601 aaatagttgt aatggtttgg ggtaataaag aggattgtga tataatttca tttttgaaag
      661 caataggaag tttaatgcca ccacctccat cgggatcacc tggcccattt gcattaacag
      721 aaaatcactt actagagcgt attttagaag atattggatt taaaatatta aacgttaaag
      781 atgtagattc catttgggat tatccagata cggaaactgc aattaaagga ttactttctg
      841 ccggaccttc agcaaaagct attgaacatg caggttttga aaatgtagta aaatcaacct
      901 taggtgttat taagtcttac tctaaaagca atggtcacat tgtatttgca aataaattta
      961 gaattgtaat tgcagaaaaa taaatattta tacatttcaa ttttatttag aaaattcaat
     1021 attccaattt cacatcataa taatctcatc ataatcatac ccggctcgga ctaaagctct
     1081 ttttgctgca tcaaatgcaa acggtgtagg attgggcgga tgtagtggaa cttccggagt
     1141 tttttcaagt tgatttaaaa gctcttgagt ttgagattgt gtcagcaact cacagacatc
     1201 ataaagtcca cccggtttct cctcttcaaa gtcatgtttt tcgagaatat gttttattac
     1261 tttaatcagg tcaggagttg gtggtggaac cattaacgct gtcaatgcac catgttctaa
     1321 tcggaattgt ggtaagattt cctgtatctt gtttgcatca atagcttttg ctgccggaaa
     1381 taaaatattt tcttccattt ttatatgtcg taacaaacct gaacgaaaag gcatataaaa
     1441 tgtcatatca atttcaggag ttgtgatggc tttgtctaat agtttttcca aacgatgatg
     1501 atcgttagtg aaaaattgat gtaaaagttt gttcatttta tctcagtgta aattataaat
     1561 cacaaatttc gtaagatttt ttaactttta aagatagaat ttctttagac aactattctt
     1621 atttttgcat cattaaggta tatgagtaaa atttctgaaa aagaagcagg agaaaagatt
     1681 aaacaattga ccgttgatct taatcatcac aattatttat actacgtaaa ggcccaaccc
     1741 gtcatcagtg attatgaatt tgatgttatg cttcaggaac tgatagctct ggaaaaacag
     1801 tttccaaact tactgactac cgagtcgcca tcacagcgtg taggaggatt tatctctaaa
     1861 gaatttgtaa cggttactca tagttatccg atgatgtcat tgggtaatac ttactcagaa
     1921 gaagagctgc gcgatttcga tgaaagagtc aggaaggtaa ttggtagcga ttttgaatat
     1981 gtatgcgagt tgaagtttga tggtgtagct attggtctga cataccagaa cggaaaatta
     2041 cttagagctg taacacgtgg tgatggtgtt cgtggcgatg atgtaacaac taatgttcga
     2101 actattcgct caattcctct aagcctcatg ggcaacaatt ttccggataa gtttgaaata
     2161 agaggtgaaa tcattctcga taaaaaaaca tttgaaagaa ttaatgctga acgtgcagac
     2221 attggcgaga caccttttgc caacccgaga aattctgctg ccggcaccct caaactacaa
     2281 gacagcagtg aagttgccag acgtaaactc gattgttttc tctatgcact ttatggtgat
     2341 tttgattttt ctacacatta tgacggactt aaaatggctg ctgattgggg atttaaaatt
     2401 tctgaacact atcaactatg caaatccatt gaagatgttt tgacttttat tgaatcttgg
     2461 cggaagaaga gagatttact tccttttgat attgatggca tagttattaa agttaacaat
     2521 ttccgtcagc aagaagaatt gggttttacc gcaaaatcgc ctaaatgggc aattgcctac
     2581 aaatacaaag ctgaatcagc ttcaaccatt ctcaatagtg ttgtatatca ggttgggcgt
     2641 acaggaacaa taacaccggt agccaattta aaacctgttt tactcgcagg cacaacggtt
     2701 aagcgtgctt cattgcataa tgccgatcag attcagaaac ttgacttaca tatgcacgac
     2761 acggtttttg tagagaaagg tggagaaatt attcctaaaa ttaccggagt agatttctca
     2821 atgcgtcaag caggagccaa gccggtcagt tacattacac attgcccgga gtgtggtaca
     2881 gaacttattc ggcttgaagg ggaggctaat cattattgcc ccaatgaaga gggatgccca
     2941 ccccaaataa aaggtaggtt agtgcatttc acctcacgta aggctatgaa tattgatgga
     3001 ctcggtgttg aaacaattga ccaactagtt gaagccgaac tactaaaaaa cgttgctgat
     3061 atttatcatc ttaaatccga aaagttactt caacttgaaa gaatggggga gaagactgta
     3121 agcaatttat tcgaagcaat tgaaaaatca aaacaagtgc cttttgaacg tgtactcttt
     3181 gccataggca ttagatttgt tggtgataca acagcaaaaa aattggcacg tcattttggt
     3241 tcatatcaaa aaatcagtca ggccacttta gaagaattga tacagacacc ggaaatagga
     3301 gataaaatag caacaagtat ttttgacttt gtacgagagt tagagaacat taaaatggta
     3361 gaacaactgc aaagtgccgg attacaattt aaacttgata aagctgcaat gcaagaggct
     3421 ggcagtaaac ttaaagggtt aagttttgtg attacaggag tatttcagaa atttgagcgc
     3481 gataaattaa aagagacaat tgagataaat ggaggaaagg tttcaggctc gctcagctca
     3541 aagacaagtt atctgcttgc aggtagcgac tgtggacctg ctaaacttga aaaggcagag
     3601 aaacttaaaa tcaacattat ctctgaagat gatttcctaa aaatgcttca atgaaaatag
     3661 gtaaggcaat aaaacaaaaa gctgcaagct atttatttta ccgtgaattg cagcgtcagc
     3721 atcatcagcc acgcaccaca agtttcagta atgcgcgcac tattggtatt ttgtatgatg
     3781 ctactattga gcgtaattat gaaattgtaa aagcctatgt aaaacatctt cgtgacgatt
     3841 ttaagaaaga tgttcttgca cttggatatt atgatggcaa tgaactgccg ccaatgcgat
     3901 tttcaaaact tggacttgat ttttttacca agaaaaatct caattggcat ttcaagcctt
     3961 cacatcctat gatttccaag tttgttaatc tcgattttga ttatctgatt tgtcttaatg
     4021 tagatcgttg catgccgtta agatatatca gtgctgcaac aaaagcaggt tttaaaatcg
     4081 gtcgttatga aaaagggtat gcggggcttt acgattttat gattagtgtt aatggtcagg
     4141 ttacactacc acagtttata gaccacgtaa ataattatct caaacatatt ggacacgata
     4201 aatgaataaa ttcacaggta caggtgttgc attagtgact ccttttttaa aaaatggcaa
     4261 aatagatttt aatgcgcttc gcaaggtagt taatcatgtt attaaaggtg gtgttgagta
     4321 tttagttgta atgggtacta caggtgagtc ggtgacgctt tcaaaagaag aaaagaaaga
     4381 tattttaaaa tttattagtg aggttaataa taaaagaaca catattgttt tcggagtagg
     4441 gggaaacaac acacttgaaa ctgcacgtga attagaacaa atagatgccc gtatggcaga
     4501 tgccattctt tcggtttctc cttattataa taagccaaat cagtcaggca tagtgcagca
     4561 ttacaaaacg ctatcaggtg tatctcagtt gcctattatt ctttataatg taccgggtag
     4621 aacaggaagt aatctattgc cacaaacaac gcttgaaatt gctgcaagtt gtaaaaacat
     4681 aattggtata aaagaagctt caggtaatat ggagcagata atgtatctca ttcagcatag
     4741 aaacaaagga tttttagtta tttccggtga tgatgctatt acaatgccgt taatagctgc
     4801 aggtgccgat ggtgtaattt ctgttattgc aaacgccttt ccaaagcagt tcagtacaat
     4861 ggtacgactt gcgctacaag gtaaaattac tgaatccaga aaactgcatt accaacttct
     4921 tgacttaata ccattattgt ttgccgaagg tagcccttca ggaattaaat atgttttgtc
     4981 gcagttagat atctgtcaga attatttcag gcttccggtt gcatcaatca gcaaatcatt
     5041 ggcacaaaaa attaatacag ttattaaagg catctgattt agataatgcg ttacttgttt
     5101 tatatgggtc atccggccca ttttcatttg tttaaaaata ccattattgc attacagcaa
     5161 tcaggtcatg aagcaatgat tctcatcaag aaaaaagata ttcttgaaga tttgttgaga
     5221 catgcaggta tgcaatacat aaacatcaat cctgaaggca gaagggatga taaattttcc
     5281 attgcattaa aattacttaa acgcgatttt cagtttttaa aaatttgtct tcgatttaag
     5341 ccacagataa tgattggtac ttcggctgaa attgcacatg ttggaaaatt actcggcatc
     5401 agaagtattg tagttaatga agacgatgca gaagttgttc catggtttgc aaaattagct
     5461 tatccctggg caacaaatat tcttgcacct tattgttgca gtgtaggtaa gtgggaacat
     5521 aaaaaaatcg gctataaagg atatcatgaa ttggcatatc tgcatccgtc caatttctcg
     5581 cccaaaaaag aaaacataaa aaatttaggg gatgaacctt tttttatact tcgttttgca
     5641 aaactaacag cacatcatga cgaaggccgt aagggtataa atactctaat tgcagagaaa
     5701 ttaataaata tgctttctaa gcatggcagg gtttatatca cttcagagcg agagttagaa
     5761 tctcagtttg agcaatatcg tattgcaata aacccgttgc atattcatga tgcgctttac
     5821 tttgctcaga tgtatattgg cgacagtcag actatggccg ctgaagctgc tgttttagga
     5881 acacctgcca taaggtttaa tgactttgtt ggtcagatta gttaccttga agaacttgaa
     5941 aaagagtttg ctttaacgtt tggctttaaa actagcgatg cagatgccat gcttgttcac
     6001 gttgaaactt tattaaatca acagaattta aaactgcaat ggcaggaaaa acgttcaatg
     6061 atgcttaatc aatgtattga tttaaaccaa tttatgatca aacttttaac tcaataacat
     6121 cttaaaaagc cttaaatagt tgattgaatt ttgtttaaaa cgatttgaag catacacttg
     6181 cacaattcga aattcgtgct tatcttagca aaccaaaatc gaattatttt aaacattttt
     6241 caactttttt taagtattga tttttaaaac cgataacaca gaggcaaaac ttttgcagtt
     6301 agaggagttt gctgaactgg atgcagtgtt ggctactaag gccaaaaatg atgtccgtaa
     6361 tgaaaggttt gtctttaatt taattgaaaa atacgttgac acatcacgta gtgattgctt
     6421 cacttttgca gctaacaatc acgatgatgc aaatcaattg gctgatgaaa cataccatgc
     6481 agttattcac tttaaaagag taaataattt tcaattcata aataaacaat tcgaggcaat
     6541 caataccaag ttacctaaag gtgggatttt tgtcagtaag gtcgaaacaa acgacctgcg
     6601 caaacaacgt ctgatggaaa aatatccacc atatctaaac aaagtttatt atttctttga
     6661 ctttgttttt aaacgggtat ttccaaagct accggtaaca cagcaaattt attatgcaat
     6721 cacaaaaggg cataatcgcg tgttgtctaa gactgaaatg ttgggaagac tttattcatg
     6781 cggatttaaa gttatagaag agcgttatat aggtaataga ctttatattg tttcagaaaa
     6841 gttcagggat cctgcttatg atgaagaccc aagttatggt gtcatttatc cgatgaaacg
     6901 cgttggtaaa ggcggaaaaa ttatcagcgt ttacaaattc agaaccatgc atgcctatgc
     6961 agaatatctg caggaatatg tttatgaaaa aaacaacctc gaagagggcg gtaagtttaa
     7021 agacgatttc agggtaagcc gtgttggtcg catgcttcga aaatattgga ttgatgagtt
     7081 gccaatgatt ttgaatgtac ttaaaggcga tttaaagatt gttggtgttc gcccgttgag
     7141 cagacattat ttaagtctgt attcaaagga acttcaggac atgcgtaaaa agttcaaacc
     7201 cggactaatt cctccatact atgttgacct acctaaaaca atggaagaaa ttatggattc
     7261 agaaatgcgc tatttaaaag cctatcagaa aagcccattc ctgacagatg tacgttattt
     7321 cttaatggtg ttttacacca ttcttttcaa gcgtgccaga agcaaatagc ggacaagtat
     7381 tccttaaatt atttttttca tctttgcgca atcataattt aaaattttat gaaagcaagc
     7441 cgtttggttt ttattttgat tttgtctttt gcatttcaat tgaatatatc tgcgcagtat
     7501 gtacctcagt cagtattcaa taaaccactt tatgatttta tggatgactt agctaatctt
     7561 catatcatca cacttaacga tgcaataaaa ccatattcca gaaaacaaat tgccgaggca
     7621 ttacaaacgg ccgaatcaca aaaagaaaaa ttaaatgcga cacagcaaaa acaacttgca
     7681 ttttatttaa aagattttaa taaagaatta tttacaggta agggatttaa aaaacgtttg
     7741 gatttgtttt attttaaaga ctcgttgttt aatattacag ttaacccagt tggcggattc
     7801 agttattata aaaatgaaaa tgacaatatg tatcgccaat atgcaggtgg agaattctgg
     7861 gcaactttag gaaaagggct aagtattttt ggaagctatc gcgattatac tgacagtaga
     7921 gcagttgcag atgataaata tctgaacgac ttgcctggat ataattataa atataaaggt
     7981 gctgttggct cgcctaaacg tggaggaagc tttgatgaaa cccgtggtgg cattgcctat
     8041 gcttggaagt ggggtcatgt tggaatagta aaagataatt ttgaatgggg cagtaataat
     8101 catggtgcaa acattatttc aacaaaggcg ccatcttttg ctcgattaga acttaagctt
     8161 aatccaaaaa agtggtttga gttcaactat tttcacggat ggctttattc tgaaattaag
     8221 gatgattctg cagcttattt ctcaggcaat ccgcctaacc acagacaagt atatttttca
     8281 aaatattttg ctgccaacat gttcaccgta aagcctttta aacaattcga tttcagtttt
     8341 ggtaattcta ttgtttatag cgatcaactg gaagttgcca tgtttattcc gtttatgttt
     8401 tttaaatcgc ttgatcatag taaaacaggg cagggtagta actattcagg tcaaaactct
     8461 caattgtttt ttaacatcag ttcacacaat attaaatata cgcatcttta tatttcacta
     8521 tttgttgatg aaattgcatt tgggcgaatg atggataaaa atgagcattc aaacttttac
     8581 agtcttaaag caggcgctgc tgtaatgtta ccgttttata cttcagctac ttttattgca
     8641 gaatatacaa gaacaaatcc ggttacatac agacactttg tgaataccac cacctacgaa
     8701 tctaatagat ttaatatggg acattatctt cgcgacaatg ctcaggaaat ttatcttggt
     8761 gcacgttgcg cgccaatttc aggtttatat atttcagcag gatacacctc agcagccgtt
     8821 gggcctattt atccctatac aggcaaagat aaatctggtt tgggtctgcc atttttaaaa
     8881 acaaaagaat atgagtttac atcacttgat ttgaatataa aatacgaatt atttaatgac
     8941 atttttttat ctgcaggtgt taaaattgat gataataaag gtgctatggc taacgtttat
     9001 actgcaccat tttattatgg aaaaaatggt aaaacaacaa cacttttcgg tggatttaat
     9061 ataggattct aaaaatgaac tttgataatt taacaacagc actcctcatc attgcgccaa
     9121 cttttatcgt tgccggtggc atgtatatgc tcataaaaaa tatgttggaa cgcgattaca
     9181 gacttaaact tcttgacgca agacgactga tacaaaaaga tatgctgcca ttaaaacttc
     9241 aggcagtaga aagaatggtt gtatttctgg aacgcataca accggagaat atcttattca
     9301 ggattttaca acccggcatg aatgtgcgag accttcaggt tgatttactt gctgccattc
     9361 gtcaggagta cgaacataat gttagtcagc aagtgtatgt ttctgtacaa agctggtcat
     9421 taatcacaaa agcaaaagat gacataattg caacagtaaa tcttgccgca gattctttgg
     9481 acaagaccga aaaggcaatt gtgctgagtc aaattatttt gcaacgttca tcagcaggca
     9541 acaattttgc aggggcggca attttatcac tcaagagtga ggtagatagt ttgttgtaaa
     9601 tctctttttt ataccttagc ccgacaaggg ttatgtcttt ttcaacacaa atcatcttta
     9661 aaactgctgt aaagcttcaa cttcaaaatt tgctgaagtt aattcccgct tcaccaaatg
     9721 gtgtgacaat actttgtttc catcgaattt catcacaata cgattatttc tggcagccta
     9781 tttatcctga aacctttcgt ttgatgctgg agtctttagt taaggaatat caaattatcc
     9841 caattagtca gatagaaaat aaatctttca gaagcaccaa gccaccgttg gttttaagct
     9901 tcgatgatgg ctacaaagat tttatagaag aagcaatacc agtgttacat tattatgagg
     9961 cgccaagcaa tcacaacatt gtgatagatt gtgccgatgg taaaagttat atctggacac
    10021 agaaactgaa tcatctgttt aacttgttgc gaaatagcag ccgcttttca ataaacattg
    10081 cattagacaa aaactttgta ctcgatatta aacaaccttg caaaaattgg ttcgctcatt
    10141 attataaaac tttattttat ctgtttaaac ttgatgtaat agatcggtct gaatgtattg
    10201 cagttttaga gcagtatctg aatattacag aaactgatta cccgataaag atgatgaact
    10261 gggatgatat taagtttata gctacaaata atgttgaaat cggttctcat actgcatctc
    10321 atgttagtct ttcggctgtt aagaatgatg taacgtttga gcatgaatta aattattcca
    10381 aaaaaagaat tgaagaaatg cttaaatgcg aatgtcctgt tatagctttg ccaaatggtt
    10441 tatcaaatgg cgaggctaat cagaaaatat ctgaagctgg ttataaaaca gtactctgtt
    10501 tggctggatc gggaagtaat gcaggaactt ctacagataa aattagattt tacaacagat
    10561 taaacatgat tcaagagccg aatagttata tgaatgtcag aattaaactt tacgaaaagg
    10621 gctttataaa atgagtgaag ttcaacttag gcagcttctg aaagatgaaa tagggttgtt
    10681 gcccggactg atggagaact gcttcagtat gaaggttgac aaagcttatt ttaaatggaa
    10741 gtatttagat aatccggcag gaaattttat tggttttggt gcattctttg aaaataaatt
    10801 ggtcggtttc tacggtgtca ttccggaacg gtatcagata aataatagtg aagtaataat
    10861 atttcaatcc ggagatacta tgactcactc aacacaaaga agaaaggggc tgtttgagaa
    10921 attggcaacc ttatgttata actatgttag ggagcatcat cagttagcag taattggttt
    10981 tggaggcgac aaatcaactc cgggattggt gaagatggga tggtgtcata ttgcagatgt
    11041 gaaatacttt tttattcatg cattgcattg caaatggatt aataatttta ccacagaaga
    11101 tagaaatttt aatatagttg agaaaacaga tattcatgtg attcttgata ttcaaaagaa
    11161 tttcggcttt aaaaaaaata taaaacaaca ttacagcaaa gagtttttaa aatggagatt
    11221 ggctaatcca cgatttaatt atcggtgttt tatttgttat caaggacaaa atcctgtggg
    11281 ttatgttatt tgttatgaag atgaagggaa gttgttttta cttgattatt gtagtatatc
    11341 tgttaaggtg cagaaaactt taatcaggag attatcacaa atttgtatta aaagcaagct
    11401 gaagggaatc ataacaattg caattccgaa gtcggaagag ggaaaatggc ttaaaaaggc
    11461 aggtttttta agaaatccat ttaaaaaagg cccgctcaca actaatattc catttatgat
    11521 tttgaccgac caaaagcctg aatcacaact ttgt
//
LOCUS       DAOOOI010000061        11493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1090_length_11493_cov_26.311418, whole genome shotgun
            sequence.
ACCESSION   DAOOOI010000061 DAOOOI010000000
VERSION     DAOOOI010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11493
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1090_length_11493_cov_26.311418"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="PKX59_07965"
     CDS             <1..1282
                     /locus_tag="PKX59_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HOZ90840.1"
                     /translation="IPTALSNEALYIAIGIIGATVMPHNLYLHSSLVQTRKIEKSSKS
                     IWRAIRFNIADSAIALNMAFFVNAAILVLAAAVFHKSGLFEIAEIQDAHQLLAPMLGN
                     NLAPILFAVALIAAGQSSTITGTLAGQIVMEGYINLRIQPWVRRLVTRLIAIIPAFIT
                     IHVFGERSTGELLVLSQVILSLQLGFAVIPLIHFVSDKNKMGEFAIGPIIKTLSWICA
                     IIIVGLNIKLVAEAMSDVFYSDTLLLWKILLALVCLGIGWLLLMTVFYPLISKASFRS
                     KVFHDEPSQISTPESIKYKRIAIALDFSSSDKKAIENALSVGGLNTHYLLIHAVESAG
                     ALTFGKEIEDMESEHDKQHLEHYIKTLKGMGYFAEYQIAHGNPGNVIPEIVNDFDAGL
                     IVMGQHGHKGLKDFLLGTTIDKVRHKVKCPMLVV"
     gene            1302..1763
                     /gene="smpB"
                     /locus_tag="PKX59_07970"
     CDS             1302..1763
                     /gene="smpB"
                     /locus_tag="PKX59_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOZ90841.1"
                     /translation="MAKKDFAPETVNKKASFDYFFEQKFEAGIVLTGTEVKSLRTGQV
                     NLTDAYCVFKNGELWVKNIHIAEYKQGGYANHIAKSDRKLLMHKRELEKLQTKLKTKG
                     TTLIPTRIYFNEKGLAKLEIALARGKKMFDKRQTEKEQEAKREVARVMKRF"
     gene            2052..3065
                     /gene="recA"
                     /locus_tag="PKX59_07975"
     CDS             2052..3065
                     /gene="recA"
                     /locus_tag="PKX59_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOZ90842.1"
                     /translation="MSTVQAPDKEKLKVLQAAIERIDKTYGKGTIMRMGEDAIEPVEA
                     IQTGSISLDIALGINGLPKGRVIEIYGPESSGKTTLAIHAIASVQKNGGIAAYIDAEH
                     AFDRFYAQKLGCDIENLLISQPDNGEQALEIADNLIRSGAIDIIVIDSVAALTPKSEI
                     EGEMGDSKMGLQARLMSQALRKLTASISRTKCCCIFINQLREKIGVMFGNPETTTGGN
                     ALKYYASVRLDIRRIGQIKDGDSVAGNRTRVKVVKNKVAPPFRQAEFDIMYGEGISKT
                     GELVDLGVELNIVKKSGSWFSYGDTKLGQGRDAVKQLFSDNPELADEIENKIKEAMNL
                     KTA"
     gene            3192..4187
                     /locus_tag="PKX59_07980"
     CDS             3192..4187
                     /locus_tag="PKX59_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ90843.1"
                     /translation="MTLKKHYILYFSVLIFTACTNGKKETEEPKDKIAQLTESIKADS
                     TNPKNYFDRATAYSQKSMFDLAYFDMEKAMKIKPSDPEYLMLYSDLSFRMNKVKDARD
                     ALVKVREMQPSNYDAAFKLAEIFLYTNNQDQSLHYLDTVLQKDPKNTKALLMQGFNLK
                     EKKDTAAAITAFRNAVAIDPKFYEAQIQLGLLMYLQNNKLCVDYYTNALNIKANSEEA
                     MYGLALWYQDHQEFNKAIQFYTDIIQINPKNKNAHFNLGYIHHEYLKVYDQAIKHYSD
                     AIAADPNYAEAYYNRGLCYEYVGNITAAKDDYSKALQIRKGNYPHAQEGLQRVSR"
     gene            complement(4218..5933)
                     /locus_tag="PKX59_07985"
     CDS             complement(4218..5933)
                     /locus_tag="PKX59_07985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90844.1"
                     /translation="MKKTIKLLLIFLMLFTSVAAQTPSWHWLARVGGYSGNGNGAGGP
                     DEWVKDVKTDAQGNVYVCGRVCYGANFNGDTMTTYQGYFSLFLAKLNCAGDLVWVKTA
                     GNTSNLYNTEAYGLALDNYGNIYLTGYLGAHPLSPCQFFDTLITEETYDMFLAKLDTA
                     GNFKWVKLAGPGPGTLGTGGRQIQITDDDMINVIGSGGSGVIFPGYNYFSLSWCFTAR
                     FDTSGNINRFQNLGSVYGPFYRDCKISPTGDQYITGYFVADSVVIGDSTLYLTNAPNV
                     GIDLFLAKFDSSGNFKWVKQIGTPSANGMKGYGVDIIGSDIVLTGMVHPGTIIGSTTI
                     TNPLYLLNSVVPFVARLYNQDSVVWVSSIQCSSAYGMPTGGVSVYNNQIATAGMFLGK
                     AVIGSDTITAVSYRDIFLSIMDASTGNFTKGVNLPGTGSSEEPQCITHDSSGNIYVGG
                     SYDGTLTVNGINYPYQGGNSDGFIAKWGSNCTVGLEEQVPLPAENLFLYPNPATSEIK
                     IQNKEQLIVAIEIYDMVGKKIMYRKVSGKESTIQIDVSSFSPGMYVVKTKGKTNMLTA
                     KFVKE"
     gene            complement(5942..11401)
                     /locus_tag="PKX59_07990"
     CDS             complement(5942..11401)
                     /locus_tag="PKX59_07990"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ90845.1"
                     /translation="MLFLLLPGFLQAQYAVVGNSEDCMNNISVYSVSGTPLPATYIWN
                     VTGGNVVSTGTGTVSIDWSSVGSGSVGVTLYDVSGAVIGTPSLTVTVNPLPQPVLTTD
                     FTNNCVIYKEHEGQVIPTVVNDTCWHVCENGIVHYFANPVNVGSSFNWNVTGAQSFVA
                     ISNNEIEVIWGSAGNGTVSVTETDGNGCVNTVEKCIVIIESPQAIITAFPGDVDCRLG
                     GVITVCLEQNALFFGNANLGNSGSPIVSWLWDFGDGTVSTHRDEVHSWQTPGIYQTTL
                     TVTNACGCTGICYVSVEVDPVNGVNIECPTPVCQGDTGKYYTDAVNCSAYNWVVTGGN
                     ILSPMPYGSTIQVQWTGGNGNGILCLDNTNGTCGNYCPSETCVEIPIISSTSSIDGPS
                     LVCIGSQTKYNVPAMTGCVYNWQITGSSGTIVSGQGTNEIMVDWTSAGTSTISVNYNN
                     ALLNCGGTANLTVETKQPFSINGPTKGCPGVDVVLTANNPVNVLLDWTITDASQNIIY
                     VGSGTGVPDFTITGWSYPSGTYIISALDNSNSYCNYTASITIEIKDPPPSPAMPVGDN
                     PICPGTVHLYSGTTMNAGYYLEWSVTGGTPTIGTGNELSIGWINTTPPHNYSISLVQV
                     ESSTGCKSLPTVLPIQERLYINPVITGNFTPCINKQVTYQISNAVPNGNYIYDQLEWV
                     IVPATAGSVISGQGTTTVTVQWNNQPSPVNVYDTYLQVKTSLCGSPPFPYPNPLFDPP
                     NISLGGAPALAITAIPNPACENTPITFTATPALPGNYTWSFGDGSSGNSPLNAVHTYS
                     LTQGASLTTFDVTLTVDDGGCQSYASTKIDVNPAPLYSLTLSATTFCPPAVTNITASL
                     VQQSGSFNYSWSNNASNNSNVNTLNYLDAQPFDVTITNTITGCARTESFSISTCSPNP
                     PLCNPTPLLYIGFTTSPASDCFTVNFVGSINPTPASYLWDFGDNTSDNTTLTPSHTYS
                     TAGQFSACLYAFDNTGLQYCDNYCLPVSVPLFPNFITSLHCSSTPGSYDVEFIDLSSF
                     TIPITTWQWFSGTTQFSTSNHPTITLAAGTYNIKLKIGNGTDFCERTLTINIPAPPSP
                     AFTVAGGPFCEGQTVVQFSASNTNVHHALWDFGDQATYNPQNPAATINAQRVFESGLS
                     IYSVNLTVTDNYGCTYSSTQQVTVNDNNLGGSISPVLISFCPGGNGSLALDYITIPGG
                     PYTQPTSWLWTGGVTTPTLTVNQTGQYFVTVSDNNGCSYSPASPGQAAVVNVPKPFIW
                     GDLTLCEGDNINLDGNQGSAVQYNWTIVDPNNVTNTFSQSSIHIAGAVAGTYHVTLDI
                     TANGCTRSTSVDVLVNPLPPLPIIQSNVPQACEGQPIDLTVINAGSYWVNWSNGASGP
                     VITVYTAGAYQATLTDANGCTNHATFEVHENPFSGFMMTGCVQYCDTVPVTVFGSGSL
                     SFNKWIWYVDGVAQASGQQSPVQDLNMGLLSPGVYVVTLQLVVGYPDGTVCEMMSDAL
                     EITIIPCPCRLYPQGKIYCMNEVNGDVQLLNNYHFEIVTNYGCSANPNVMVSSPDGGV
                     SMLPNSNPPQLLTGILSTYSAYPDNVCFDINITDSNKPMCSCNYRYCMKLPKCGVPTG
                     CSTEVKIDNVQCIGHDANGNRIYSFTLTLLNSPQLFSMFTAQGGVLGAMPATVGPGTT
                     IINSSITVNNNASHFCITLHGYNFTTYTACKTTGCSGNLPPCGIPPSRNGEKDTTTPL
                     RVSYQNDANDAVQNTSLSLMPNPAKNKVTLHYSTRSGGIITIASAVGQIIYNENVATA
                     GMSELSTQQWLPGIYFVTLQSNAGDCLVQKLVIIK"
BASE COUNT         3549 a         2260 c         2334 g         3350 t
ORIGIN      
        1 aatcccaaca gcattgagta acgaagcttt gtatatagct attggaatta taggtgccac
       61 tgttatgcct cacaatcttt atctgcattc gtcattggtg cagacaagaa aaattgaaaa
      121 aagcagcaag tcaatttggc gagctattcg atttaatatt gctgacagtg ctattgcatt
      181 gaacatggca ttctttgtca atgcagctat tttagtactt gcagcagcag tgtttcataa
      241 atcaggactt tttgaaattg cagaaattca ggatgcacat cagttgcttg cgcccatgct
      301 gggaaacaac cttgctccta ttctttttgc tgtagcattg atagcagcag gacaaagttc
      361 taccatcacc ggaacattag caggtcagat tgtgatggaa ggatatataa accttcgtat
      421 tcaaccctgg gtgcgcagat tagttacacg ccttattgcc attattcctg catttatcac
      481 cattcatgtt tttggcgaaa gaagcaccgg tgagctgtta gttttaagtc aggtaatttt
      541 aagtttacag ttaggctttg ctgtaattcc actcattcat tttgtaagcg ataaaaataa
      601 aatgggcgaa tttgctattg gccctattat aaaaacgctg tcgtggatat gtgctataat
      661 tattgtagga ctaaacatta agttggttgc agaagcaatg agtgatgtat tttatagcga
      721 cacactatta ctctggaaaa tactattggc tttagtttgt ttaggaatcg gatggttgct
      781 attgatgaca gtgttctatc ctttaataag caaagcatct ttcaggtcaa aagtatttca
      841 tgacgaaccc agtcagattt ctactcctga aagtataaaa tacaaacgca ttgctattgc
      901 gttggatttt agttcaagcg ataagaaagc gattgaaaat gcattaagtg ttggtggact
      961 taatactcac tatttgttga ttcatgctgt ggaaagtgca ggggcattaa ccttcggaaa
     1021 agaaattgaa gacatggaga gtgaacatga caaacaacat ttagaacatt acattaaaac
     1081 gttgaaaggc atgggttact ttgccgagta tcagattgca catggcaacc cgggaaatgt
     1141 tattccagaa attgtaaatg attttgatgc aggcttaatt gttatgggcc agcatgggca
     1201 taaaggatta aaagattttt tacttggaac tactattgac aaagtgcggc ataaagtaaa
     1261 atgtccgatg ttggtagtat aattttactt ttgcaaccgc aatggccaaa aaagattttg
     1321 ctcccgaaac cgtcaacaaa aaagcttcgt tcgattattt tttcgagcag aagtttgaag
     1381 ccggcattgt acttacaggt actgaagtaa aatctttgcg taccggacaa gtaaatttaa
     1441 ctgatgccta ttgtgttttt aaaaacggag agctttgggt aaaaaacatt cacattgcag
     1501 agtacaagca aggtggttat gctaaccaca ttgccaagtc cgacagaaaa ttactgatgc
     1561 ataaacgtga gttagaaaaa cttcaaacca agttaaaaac taaaggaaca acattaattc
     1621 caacacgcat ttattttaat gaaaaaggat tggcaaaact tgaaattgca ttagcaagag
     1681 gaaagaaaat gtttgataaa agacagacag aaaaagaaca ggaagcaaaa agagaagttg
     1741 cccgcgtgat gaagcgtttt tgagaatctg aacaaatatc atctacatca taaaatttat
     1801 taaaattcag ctaataagct gaatacatgc cacttacgta aattcaccca aaaccttaaa
     1861 acgtgctgct tgattgcatt acgaccatta aagttgttga ttttaaatta aaattcaggg
     1921 ctaattgaca gatttacaat cacaacgagg acggcattgc cgctgttgaa aacaaaatat
     1981 tgaattttca taaatcagaa ttggctaaaa ccattacttt gtcgctataa acattcaaaa
     2041 aaataagaca catgagcaca gtacaagcac ccgacaaaga gaaattaaaa gtattacaag
     2101 cagccattga acgcattgac aaaacctatg gtaaaggcac catcatgcgc atgggcgaag
     2161 atgccattga acctgttgaa gccattcaga caggaagtat ttcattagat attgcattag
     2221 gcattaacgg tcttcctaaa ggacgtgtaa ttgaaatata cggacccgaa tcttcaggta
     2281 aaacaacatt agccattcac gctattgcca gcgtacagaa aaatggtggc attgctgcct
     2341 atatagatgc agaacatgca ttcgatcgtt tttatgcaca gaaattaggt tgtgatattg
     2401 agaaccttct tatatcgcag cccgacaatg gcgagcaggc acttgaaatt gcagacaatc
     2461 ttattcgctc aggtgcaata gacattattg ttattgactc tgttgctgct ttaacgccaa
     2521 agagtgaaat tgaaggcgaa atgggcgatt ctaaaatggg attgcaggca cgtttgatgt
     2581 cgcaggcatt gcgtaaactt actgcaagca tcagccgaac taaatgttgc tgtattttta
     2641 tcaatcagct tcgcgaaaaa attggtgtta tgttcggaaa tcccgaaaca acaaccggtg
     2701 gtaatgcatt gaaatattat gcatcagtgc gtttagacat tcgcagaata ggacagataa
     2761 aagatggtga cagtgttgca ggaaaccgca cccgtgttaa agtggtgaaa aacaaagtag
     2821 ctcctccatt ccgtcaggca gaatttgaca ttatgtatgg cgaaggtatt tctaaaacag
     2881 gagagcttgt tgaccttggt gttgaattaa acattgtaaa gaaaagcggt tcgtggttca
     2941 gctatggcga taccaaactt ggtcagggac gcgatgctgt gaaacaactt tttagtgaca
     3001 atcctgaact tgccgatgag attgaaaata aaattaaaga agcaatgaat ttaaaaactg
     3061 cttagaaaaa taattatttg tttattggta aaagaaaaga gctgccggtt ttcggtggct
     3121 tttttcattt cataccaatt gtatatgatg gattgtgttt aaaccgatat ttgcaccaca
     3181 ataacattag aatgacattg aagaaacatt acatacttta tttttcagtg ctgattttta
     3241 ctgcctgcac aaatggaaaa aaagaaacag aggagccaaa agataaaatt gctcagctta
     3301 cagaaagcat caaagccgac agcaccaatc caaaaaacta tttcgaccgt gccactgcct
     3361 attcgcaaaa gagcatgttt gatttggctt attttgatat ggaaaaagca atgaaaataa
     3421 aaccatctga cccggaatat ctgatgcttt attcagacct tagtttcaga atgaataagg
     3481 taaaagatgc acgcgatgca ttagtaaaag tgcgcgaaat gcaaccatca aactatgatg
     3541 ctgcatttaa attggcagaa atatttttat ataccaacaa tcaggatcag tcgttgcatt
     3601 atttagatac agttttacag aaagacccta aaaacaccaa ggcattgctg atgcagggct
     3661 tcaacttaaa agagaaaaaa gatacagccg ctgccattac agctttcaga aatgctgttg
     3721 ccattgaccc taagttttat gaggcacaaa tacaattggg tttactgatg tatttacaaa
     3781 acaacaaact gtgcgttgac tactacacca atgcattaaa cattaaagcc aacagcgaag
     3841 aagccatgta tggtctggca ttgtggtatc aggatcatca ggagtttaat aaagccatac
     3901 agttttacac cgacattatt cagataaatc ctaaaaacaa aaatgcacat ttcaaccttg
     3961 gatacataca tcatgaatat ctgaaagttt acgatcaggc aatcaaacat tattccgatg
     4021 ccattgcagc agaccccaat tatgcagaag cctactataa ccgtggttta tgttatgagt
     4081 atgttggaaa tattacagca gcaaaagacg attacagcaa agcactgcaa ataagaaaag
     4141 gtaattatcc acatgcacag gaaggactgc aaagagtgtc gaggtagagg agatgaattt
     4201 ttgagacaca ttctacccta ctccttcaca aactttgctg ttagcatatt tgttttgccc
     4261 ttggttttta ctacatacat gcccggtgaa aatgacgaaa catctatctg aatggtagac
     4321 tctttaccag aaacttttct gtacattatt tttttaccaa ccatatcata aatttctata
     4381 gcaacaataa gttgctcttt attttgaatt tttatttctg atgttgcggg gtttggatat
     4441 aaaaataaat tttctgcagg caatggcact tgttcttcta aacctaccgt acagttgcta
     4501 ccccacttag caataaaacc atcggagttg ccgccttggt agggatagtt tattccattt
     4561 acggttaaag tgccatcgta actaccacca acataaatat tgccgctgct atcgtgagtt
     4621 atacactgtg gctcttcact actgcctgta cccggtaaat ttacaccttt tgtgaaattg
     4681 cccgttgagg cgtccattat tgataaaaat atatctctat aacttactgc tgtgatagta
     4741 tcgctcccta taactgcttt tcctaaaaac attcccgcag ttgctatttg attgttatat
     4801 acagaaacac cacctgtggg catgccataa gcagatgaac actgaatgga cgaaacccac
     4861 actacactat cttgattata taacctagct acaaaaggca ctacagaatt taaaaggtat
     4921 aatgggtttg taattgtagt tgatcctata attgtgccag ggtgtaccat gcctgttaaa
     4981 acaatatcag agccaataat atctactccg tagcctttca ttccattagc tgatggagta
     5041 cctatttgtt tcacccattt aaagttacct gaggaatcga atttagccaa aaacaaatct
     5101 attccaacat ttggtgcatt agttaagtac aaagtagaat caccaattac aacagaatct
     5161 gcaacaaaat agcctgtaat gtactgatcg cccgtgggac ttattttgca atctctataa
     5221 aatggaccat atacactgcc taaattctgg aatctgttaa tatttcctga agtatcaaaa
     5281 cgcgcagtaa aacaccaact aaggctaaaa taattatagc cgggaaaaat aactcccgat
     5341 cctcctgaac cgataacatt aatcatatcg tcatcagtaa tttgaatttg tcttccgcct
     5401 gtacccaaag ttcccggtcc cggacctgct aatttaaccc atttaaaatt tcctgccgta
     5461 tctaactttg caagaaacat atcgtatgtt tcttctgtta taagtgtatc aaaaaactgg
     5521 caaggactta atggatgcgc accaaggtag cctgttaaat aaatattgcc ataattatct
     5581 aaagcaaggc cataagcttc tgtattatac aaattgcttg tgttacctgc agtttttacc
     5641 caaactaagt ctccggcaca gtttaattta gccagaaaca agctaaaata accctgatag
     5701 gttgtcatgg tgtcgccatt aaagttagcc ccatagcata ccctgccgca gacatataca
     5761 ttgccctgtg catcggtctt tacatctttt acccattcgt caggtccacc ggcaccattg
     5821 ccattaccac tgtagccgcc tacacgtgcc agccaatgcc aacttggtgt ttgtgctgcc
     5881 acacttgtaa acagcatcaa aaatatcagc aataatttta ttgtcttttt cattgtttgt
     5941 gctatttgat gatgactaat ttttgaacca aacaatcgcc tgcattgctt tgcaaggtaa
     6001 caaaataaat accgggcagc cattgctgtg ttgaaagttc tgacatgcct gctgttgcca
     6061 cattttcgtt atatattatc tgcccaacag cagaagctat ggtgataatg cctcccgaac
     6121 gggtgctgta atgtagtgta actttgtttt tagccggatt aggcatcagg ctaagcgatg
     6181 tattctgaac agcatcgtta gcatcatttt gatagcttac cctcagtggt gtagttgtgt
     6241 ctttttctcc gtttcttgat ggaggaatac cacatggggg caaattaccc gagcaaccag
     6301 tagttttaca agcagtgtag gtagtaaaat tgtagccatg caaggtgatg cagaagtggc
     6361 ttgcattatt gttgactgtt atagaactgt tgatgatggt tgtgcccggt ccaactgttg
     6421 caggcatagc gcctaaaaca cctccttgtg ccgtaaacat ggagaatagc tgtggagaat
     6481 taaggagtgt gagtgtaaaa ctatagattc tgttgccatt ggcatcatga ccgatacatt
     6541 gcacattgtc tatcttaact tctgtgctac agcctgtggg aactccgcat tttggcaatt
     6601 tcatacaata gcggtagttg cagctacaca tgggcttgtt tgagtctgtt atgttaatgt
     6661 caaaacaaac attgtcagga taggcactgt aggttgataa tattccggta agtagttgcg
     6721 gtggatttga attcggcagc atagatactc cgccatctgg cgaagacacc ataacattag
     6781 gattggcaga gcatccatag ttggttacta tctcaaaatg ataattattc aataactgaa
     6841 catcaccatt cacttcattc atgcaataaa ttttaccttg cggataaagt ctgcaaggac
     6901 atggaatgat ggtaatttca agtgcatctg acatcatttc gcacacagtg ccatcaggat
     6961 agccaactac taactgaagc gtaactacat atacacccgg gctgagcaat cccatattaa
     7021 ggtcttgtac ggggctttgc tggcctgaag cttgcgcaac accatcaaca taccatatcc
     7081 atttattaaa agacaaactt ccactaccga atactgttac aggcactgtg tcgcaatatt
     7141 gcacacagcc tgtcatcata aatcctgaaa aggggttctc atgcacttca aaggttgcat
     7201 gattggtgca gccattggca tcagttagtg ttgcctgata ggcaccggca gtataaacag
     7261 taataacagg gccgcttgca ccattgctcc agtttaccca ataactaccg gcattgataa
     7321 cggtaagatc aatgggctgc ccttcgcagg cttgcggcac attgctttga ataataggca
     7381 atggcggtaa tggatttact aaaacatcta cagaagtgct gcgggtgcaa ccattggcag
     7441 taatgtcaag tgttacatga tatgttccgg caacagcccc tgcaatatgt atggaagact
     7501 ggctaaaggt attggtaaca ttattggggt caactattgt ccagttgtac tgtacagcac
     7561 taccctgatt gccatctaaa ttaatgttat cgccttcgca cagtgtcaga tcgccccaga
     7621 taaaaggctt tggcacattt acaactgcag cctgaccagg gctggcagga ctgtagctgc
     7681 aaccgttatt gtcgctcact gtaacaaaat actgccctgt ttgatttacc gttaatgtgg
     7741 gcgtagttac accaccagtc catagccagg aggtgggctg tgtgtaagga ccacctggaa
     7801 ttgtaatata atccaacgcc aaacttccat ttcctcccgg acaaaatgat attaatacag
     7861 ggcttatgct gccacctaaa ttattatcat taaccgttac ttgttgtgtg cttgagtagg
     7921 tgcagccgta attgtcggta actgtaagat ttactgaata aatgctaagt cctgattcaa
     7981 acacccgctg tgcattaata gtggcagcag ggttttgagg gttataagta gcctgatcac
     8041 caaaatccca aagggcatgg tgtacgttgg tgttgcttgc actaaactgt acaacagttt
     8101 gcccttcgca aaatggcccg cctgcaacgg taaatgcagg tgagggtggt gcaggaatat
     8161 ttattgtaag tgttcgttcg caaaaatctg tgccgttgcc tatttttagt ttaatattat
     8221 aggtgcctgc tgccaaagta atggttgggt ggttgcttgt gctaaactgt gtggtgcctg
     8281 aaaaccactg ccaagtggta attgggatgg taaaagatga aaggtcaata aattcaacgt
     8341 cataacttcc gggtgtactg ctgcaatgca acgaggtaat aaaattcgga aaaagcggca
     8401 ccgataccgg taagcaataa ttgtcgcaat actgtagtcc ggtattatcg aaggcatata
     8461 ggcaagcact aaattgtccg gcagtgctgt atgtatgtga aggggtaagt gttgtgttat
     8521 cactggtatt atcaccaaaa tcccataaat aacttgcagg tgtagggttg atgctgccta
     8581 caaaatttac tgtaaaacaa tctgatgccg gtgaagtggt aaagccgata taaagaagag
     8641 gggttggatt gcacagaggc ggattgggtg agcaagttga aattgaaaaa ctttctgttc
     8701 tggcgcaacc ggttatcgta ttggtgatgg ttacatcaaa tggttgtgca tctaaataat
     8761 tcaatgtatt aacgttgcta ttattagatg cattattact ccacgaataa ttaaaacttc
     8821 ctgattgttg caccagcgag gctgtaatat tagtaacagc cggtgggcag aacgttgttg
     8881 ctgaaagtgt caaagaatat aatggagcag ggttaacatc aatttttgtg ctggcataag
     8941 attggcatcc gccatcatca acggtcaagg taacatcaaa ggttgtgagg ctggctccct
     9001 gagtcagact ataggtatgc actgcattta aagggctgtt tccgctgctg ccatcaccaa
     9061 aactccaggt ataattaccg ggaagcgcag gagtggctgt aaaagttatt ggggtatttt
     9121 cacaagcagg atttggtatt gcagttatag ctaaagcagg tgctccgcca agtgaaatat
     9181 tgggtgggtc gaacaatgga ttcggataag ggaatggcgg tgagccacag agcgaagttt
     9241 ttacctgaag ataggtgtca taaacattaa ccggagaagg ttggttattc cactgcacag
     9301 ttacagtggt agtgccttgc ccgcttatta cacttccggc agtggcagga acaattaccc
     9361 actctaattg atcgtatata taattgccat ttggcacagc attggaaatc tgatatgtca
     9421 cctgcttatt aatacatggt gtaaaatttc ctgttatgac aggattaata taaagtcttt
     9481 cctgtattgg caatactgtt ggcaatgatt tgcatccagt agatgactct acctgcacca
     9541 aactgatgga atagttatgt ggaggtgttg tattaatcca gcctatagat aattcattgc
     9601 ctgtgcctat tgttggagtg ccaccagtta cactccattc taaataatat cctgcattca
     9661 ttgttgtgcc gctgtacaaa tgtacagtgc ccggacatat tgggttgtca ccaacaggca
     9721 ttgcaggtga cggtggcgga tcttttattt ctattgttat gcttgctgtg taattacaat
     9781 aactgtttga attgtctaat gctgatataa tataggtgcc actaggataa gaccatcctg
     9841 taattgtaaa atccggaact cctgtaccac tgccaacata tatgatgttt tgagaagcat
     9901 ctgttattgt ccaatccaat agtacattga caggattatt agctgtgagt acaacatcta
     9961 ctcccgggca accttttgtt ggtccattaa tactaaaagg ctgcttggtt tcaacagtta
    10021 aattggcggt gcctccgcaa ttcaataagg cattgttata atttacactt attgttgaag
    10081 ttccggcact ggtccaatct accattattt cattggtgcc ttgccctgaa acaatagtgc
    10141 cgctgctgcc tgttatctgc cagttataaa cacaacccgt catagcaggc acattgtatt
    10201 ttgtctgaga gcctatacaa accaatgagg ggccatcaat actacttgtt gaagatataa
    10261 taggaatttc aacacatgtt tcacttggac agtagttacc acaggtaccg ttagtattat
    10321 ccagacataa aatgccattg ccattgccac ccgtccactg tacctgtatg gtgctgccat
    10381 aaggcattgg tgaaagaatg tttcctccgg taactaccca attatatgca ctgcaattca
    10441 ctgcatcggt ataatattta cctgtatcgc cctggcatac aggagttgga cattctatat
    10501 tcacaccatt tacagggtca acctctacag atacataaca aatgcctgta caaccacatg
    10561 cattggttac ggtgagtgtt gtctgataga ttccaggcgt ttgccacgaa tgtacttcgt
    10621 ctctatgtgt agaaactgtt ccatcgccaa aatcccacaa ccaacttaca attggtgaac
    10681 ctgaatttcc aaggttagca tttccgaaaa ataatgcatt ttgctcaaga caaacagtaa
    10741 ttacgccacc taaacgacaa tccacatcac ccggaaatgc agtaattatt gcctgtggcg
    10801 attcaataat gacaatacac ttttcaaccg tatttacaca accattgcca tcagtttctg
    10861 ttacagaaac agttccgttg ccggcacttc cccatataac ttctatctca ttatttgaaa
    10921 tagctacaaa actttgcgca cctgttacat tccaattaaa tgatgagcca acgttaacag
    10981 gatttgcgaa gtaatgtact atgccgtttt cacaaacatg ccagcaggta tcatttacca
    11041 ctgttggtat tacctgccct tcatgctctt tatagataac acagttgttg gtgaaatctg
    11101 tagtaagcac aggttgtggt agtggattta ctgttacagt aagtgatggt gttcctatta
    11161 ctgcacccga aacatcatac aatgttacac ctacagaacc agaaccaaca gaactccaat
    11221 ctatactcac tgttcctgtg cctgtactaa caacgttgcc tcctgtaaca ttccagatgt
    11281 atgttgccgg aagtggtgtt ccgctgacag aataaaccga tatgttattc atgcagtctt
    11341 ctgagttgcc aacaacagcg tattgtgcct gtaaaaagcc gggcagtaga agaaatagca
    11401 ataaaataat tttcgatttc aaaacaattt taactgaact gttaaaacct gattttagca
    11461 atttgcctga aattgcgggg gggggggggg ggg
//
LOCUS       DAOOOI010000062        11482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1092_length_11482_cov_83.728800, whole genome shotgun
            sequence.
ACCESSION   DAOOOI010000062 DAOOOI010000000
VERSION     DAOOOI010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11482
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1092_length_11482_cov_83.728800"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..596)
                     /locus_tag="PKX59_07995"
     CDS             complement(126..596)
                     /locus_tag="PKX59_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90846.1"
                     /translation="MSRAYPIGIMPPNTEETIKKEVEIAIEYHNGNEDIFVELCDNVV
                     RSADYEEVMKNATERKAFGYMKLGQLKKVLDTGKLQETIDVIISGDKPKELSAPAGLD
                     APELSVEEMKRALENAMNNKNQFGHSKNHNKAWFDRKKKKSRNKQAKQSRKKNR"
     gene            complement(596..1096)
                     /locus_tag="PKX59_08000"
     CDS             complement(596..1096)
                     /locus_tag="PKX59_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90847.1"
                     /translation="MKKIVFLLSVIFVIGCSKNENMVPDNPASTLKAKRLAYLQQSVG
                     TFYSYDDTLGLSDSVKVTHLKDSLFLSVYSSEFDTSFIMTLDTIDADSIGYFTALPFS
                     FNVLKNVSFDDNITGCQVTNGLSTYYPEYIVFSFVIDGTYHSTLSPAMDNQPVTDFLF
                     TRQFSR"
     gene            complement(1205..1293)
                     /locus_tag="PKX59_08005"
     tRNA            complement(1205..1293)
                     /locus_tag="PKX59_08005"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1260..1262),aa:Pro,seq:cgg)
     gene            complement(1862..2911)
                     /locus_tag="PKX59_08010"
     CDS             complement(1862..2911)
                     /locus_tag="PKX59_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90848.1"
                     /translation="MESVNDFKLEDLLSYNWLLSKGYVISLPEKDNKFAIVFVARILY
                     RQVPRGKNMTASKNLSKVMDVTTRTLYKYIDCGFAIDKIFKDATYLKYTKERNDIFTK
                     PFICRIIDKSTKQEIVEFDVTIQTLYDSNLIVDISEADKADKPKLDMRKIIQLRNDIL
                     NEDIVELNDTNREDMERLAMHICEVFGTGCVTITEACDRYNVNYLTWIDWLRKNAYIR
                     QMYEEACRAAALINHTRQLSLVDNRLNALLNNGFVEETTFTYDKVFLPGRLEPVYREK
                     TKVVRRRDLDLSQLAMIKGMLYRSLISLPPSDPDDFSNWSTEEILNYVNSMDDTEKMD
                     KLSVIQTEILKNPPE"
     gene            complement(3064..3279)
                     /locus_tag="PKX59_08015"
     CDS             complement(3064..3279)
                     /locus_tag="PKX59_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90849.1"
                     /translation="MDLFNQAEEKKDYTIFNNMTDYEKRCLITTNYWKVRNGEQEIDV
                     PEEHEKYHALWKNEKARALLHKSKIIS"
     gene            complement(3279..3698)
                     /locus_tag="PKX59_08020"
     CDS             complement(3279..3698)
                     /locus_tag="PKX59_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90850.1"
                     /translation="MPINYKEYPDNWLTEIRPAVLARAENKCEECGVANQAIIKRWPD
                     GRVTYPNSAEWEMINNRVKNHDYSFTQAIKKYGFTRVVLTIAHLDHDKSNEKVPLDRL
                     RAWCQKCHLGYDMPIHVNNRKYGKKHVDNNYKLEFTD"
     gene            complement(3702..3950)
                     /locus_tag="PKX59_08025"
     CDS             complement(3702..3950)
                     /locus_tag="PKX59_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90851.1"
                     /translation="MQEQFSDNIENIDDVFKSYGLQSETIELVFVQSLLKNIKPDLQF
                     GMCDKLAERICIDTMLHKISQFNMAMEKEHDLLIKQKQ"
     gene            complement(3950..4441)
                     /locus_tag="PKX59_08030"
     CDS             complement(3950..4441)
                     /locus_tag="PKX59_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90852.1"
                     /translation="MKHTKKTDRDYKHWTDSDIETLTLNYIDKTNSELAILLNRNADT
                     IRKQLNKLKLKRPLKSEIKKIEKKVKKVDFFSCLNSGRKILEQEKRKAEAIRKRDERM
                     KQEKKWADGFLEKEKPVFVPRNIPRKPVIVNDRTVIYVDASLNEEQVQAVVQKFIEKT
                     KWM"
     gene            complement(4441..4911)
                     /locus_tag="PKX59_08035"
     CDS             complement(4441..4911)
                     /locus_tag="PKX59_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90853.1"
                     /translation="MSKFSLSVNEENLFRSSLKIGNGLTVSCETIGKHPKDVSEHLKN
                     NEAFLNLCKSDLGVVNNAIIAINTNLLVDEKFDKFLQTKERAKVVITTIVFWEEYCLR
                     KEVTADKVVKVVHLYKGLGDCATSVGMLTSEFKEYIMADEKLAMYIYTNQIFIF"
     gene            complement(4904..6490)
                     /locus_tag="PKX59_08040"
     CDS             complement(4904..6490)
                     /locus_tag="PKX59_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOZ90854.1"
                     /translation="MALKKSIQSFSNVESIEIDKIVHNGNYRKHIDEEKIIELAESMK
                     SVQLLHPIMVRDTGKGKYHLVFGERRLLAAKSLGWEKIDSRIIQNVKPDKLVEIQLIE
                     NVQREQPHPIDESETFVQLTKITSVEQIASTIGVSEQYVYDRIHLKSLNAEIKRLFKD
                     SHITLHHCLQFAKLQNAKQLELLERIKYNVPYNEKEGEHRFAYRSVKEMRRIIEKEFV
                     CLLTKAPFDIEDKRLNKSAGSCINCMKRSGANRTLFGDVQSDDLCFDTKCFWKKTSAK
                     LTTLEKQLVDAGNTVIRIDTSLIPDENEHENLVSLTEVHQIPENEVDNIDSKLFGIIF
                     SSDNIQLLGTVIKLYEKVPEVNEVETIIHNEPVKATVTPSQKRMENVKIKNGRISLKN
                     KLKEEVGFMLGAKELDKMPVSILKMLFYNIIAFNEVSELLENLKWKYDGMPVNSPEII
                     ELDIMKLVNENVNNQSYERVLMEMMIFIVFVTIENNQVYESTFKPIFELAKDIGVDVE
                     ALENTTSEDFAITIQQLLNE"
     gene            complement(6456..7037)
                     /locus_tag="PKX59_08045"
     CDS             complement(6456..7037)
                     /locus_tag="PKX59_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90855.1"
                     /translation="MTTDVTAYDNCISTYSGLRFDFINPRPDMVNVLDICKGLANKPH
                     FSGQTPEFFSIAEHCILVESLLPYDAEPEERLCALIHDGSEAYLPDMIKPIKLLLPLF
                     KDIEDGVQSCIFDYFGLDERYMKRIKKYDIMAQQIEYNVFYFNHATLANNYHICYLKP
                     KDAYLAYIKRLFYILQTIKYDSNGTKKKYSIIQ"
     gene            complement(7034..7867)
                     /locus_tag="PKX59_08050"
     CDS             complement(7034..7867)
                     /locus_tag="PKX59_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOZ90856.1"
                     /translation="MSYRVPTPNTNFDLETTGTNVLTDRIVQIALVYTNSIGMRDTRN
                     ILINPGIPIPVDATDVHGITDEMVKDASTFPEIANDIFQYMVDVTLIGFNIKNFDIPL
                     LKEEFKRCGIDWDVPKDIIDVFQMDKALYRRTLNNVYKQHTGLDIDGAHDALNDVYAV
                     DVILQSMVKYNNTEVQNFLTTSKDQVDIAGKFLLDEVGNIVFGFGKFQGNQVSKHIQY
                     VQWMNQQEFFTADTKDFCKKILAGAFRVEQLTNKPAEVVDEAKEKILRDLENAEVDPF
                     A"
     gene            complement(8007..8261)
                     /locus_tag="PKX59_08055"
     CDS             complement(8007..8261)
                     /locus_tag="PKX59_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90857.1"
                     /translation="MKLNLSEHQILVADCVTIAEFVKQLRASTGNEKINASTIHYHLK
                     NTDNLDYVEVNNVFLIVVNDKAKSFNPGSYYKGRISKMDL"
     gene            complement(8258..8680)
                     /locus_tag="PKX59_08060"
     CDS             complement(8258..8680)
                     /locus_tag="PKX59_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90858.1"
                     /translation="MLILLVAKASLGISQINPPVKINDKFCFDKQSTLELIKSDIKAK
                     SYDSLLLTSLTYEKLVVEKNNINDSIILDQATQIDNLSLIASEKDKQIVLLKNENKAL
                     KKENRKRRRKLFFSNFTIVTGVAIGVTTYAVIKAKSKK"
     gene            complement(8613..9005)
                     /locus_tag="PKX59_08065"
     CDS             complement(8613..9005)
                     /locus_tag="PKX59_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90859.1"
                     /translation="MKTEIKKEIFAFVKRDWIYIAIFAATLIITGIVFRIQSSADNTK
                     ELSGIIKAIYAEKQMVIDSLTKQNKLLQSKIDTLTWRKNVITKEIVYIKQKQNGIIKT
                     TSQKIADVDTFGSESIIRYFSNKSSGKN"
     gene            complement(9007..9237)
                     /locus_tag="PKX59_08070"
     CDS             complement(9007..9237)
                     /locus_tag="PKX59_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90860.1"
                     /translation="MFIVALIFSWFPSVFISLRFLLAKVYANLQKKNEAENNLESLLI
                     INTVMVELETLSRYFLWYGITATVLILIIKYV"
     gene            complement(9244..9897)
                     /locus_tag="PKX59_08075"
     CDS             complement(9244..9897)
                     /locus_tag="PKX59_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90861.1"
                     /translation="MQKLFSNGKILMAPAGTELDEVISKLVENAKENYSKEIVFDFAK
                     EPFPNYIIPSDVTFDQIREYCYPDITLEFKVVGNEVTEYHSNQLKEIKYTFTPEEKLE
                     LADRLCEFEKMREEQEEMKKAETKRLADIVKNTETNISIIASKYREGYEWRNVDTILA
                     FDFANKQRIYMEPGTHVVLHVEDLKAEDYQLQLNLDNMTTPVEEKTEEQVTDEDIFK"
     gene            complement(9919..10275)
                     /locus_tag="PKX59_08080"
     CDS             complement(9919..10275)
                     /locus_tag="PKX59_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90862.1"
                     /translation="MAITTVILKNSDVPSMNILLRMHWTKRAKMLEQIQWKIRSQTVS
                     RHPGKVRIVFHRFAVKLMDWDNYAGAFKFIGDALHGNKIIVDDKPAIVVEFIPKQTQV
                     DTLKQQKILVTIEDII"
     gene            complement(10419..11069)
                     /locus_tag="PKX59_08085"
     CDS             complement(10419..11069)
                     /locus_tag="PKX59_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HOZ90863.1"
                     /translation="METERKELLKMCDGKKYNVIYADPAWRFSQGINRRKDIREKFGT
                     NEELSIQYPTMSDKEIIELNVADYADDNCVLFVWTTDAHLEVAIKAINNWGFKYKTVA
                     FTWNKKRGFMGKWTVKQCEICLLATKGTAHKLLKSFKEKSYLEENKTEHSKKPNEFRK
                     RIERMFGDVPKLEMFARTRAEGWDVWGNETDKFEEEKGGKIFNNSFPPQSLFGDVS"
     gene            complement(11125..11325)
                     /locus_tag="PKX59_08090"
     CDS             complement(11125..11325)
                     /locus_tag="PKX59_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90864.1"
                     /translation="MIEIITEKVEMPSLSTKRRFQKICINNKWEVIDTHNENKTRYKG
                     AYEDVMIACHNLNKKFYRDGVV"
BASE COUNT         3130 a         2391 c         1755 g         4206 t
ORIGIN      
        1 actgattagc gtcgaaatta gacggtatat tcagttgaat tttacactct ttgtgttttg
       61 atgttgtcca ccctggctca tgctctaatg tcaactttat ttttgtttcg agttggtata
      121 acatattatc ggtttttttt acgtgattgt tttgcctgtt tgttccggct tttctttttc
      181 ttcctgtcaa accatgcttt gttgtggttc ttagaatggc cgaactgatt tttattgttc
      241 attgcatttt ccaatgcacg tttcatttcc tccacactta attcaggtgc atctaatcct
      301 gcaggagcgc ttaattcctt tggcttgtca cctgaaataa ttacatctat cgtttcctgc
      361 agtttacctg tatctaatac ctttttcagt tggcctaact tcatgtaacc aaacgctttc
      421 cgctctgttg catttttcat tacttcctca taatctgcag aacgcaccac gttatcgcat
      481 aactctacaa aaatatcctc gtttccgtta tggtattcaa tggcaatctc cacctccttt
      541 ttgattgttt cttcggtatt tggaggcata ataccgattg gatatgctct gctcattacc
      601 tgctgaattg acgagtgaat aaaaagtcag tgaccggctg attatccatt gcaggactta
      661 gtgtactgtg atacgttccg tcaatcacga aactgaacac tatgtattca ggataatacg
      721 tgcttaatcc attcgtaacc tggcaccctg taatattgtc gtcaaaactc acattcttca
      781 gaacgttgaa cgagaaaggc aatgcggtaa aatatccaat actatctgca tctattgtgt
      841 ccaatgtcat tatgaaagat gtatcgaact ccgaactgta aacactaaga aataaagaat
      901 ctttcaggtg tgtaactttc acagaatcgg ataaaccgag cgtatcgtca taactgtaaa
      961 atgtacctac gctctgttgc agatatgcaa gacgtttggc tttcaatgtg gaggccggat
     1021 tatccgggac catgttttcg tttttactgc aaccgataac gaaaataacg gacaaaagaa
     1081 atactatttt tttcatgtct ataaattaaa aaagagggta ggcagaaatt ccctctcgat
     1141 gaaacataaa aaccctgaac taataccact ctaccaatgg cacgacggaa cccaacattc
     1201 cttgtggtga agtcaggact cgaacctgaa aggacttacg ctttaactgc cacatcgctc
     1261 cgcaatgttc gtttaccaat ttcgcacctc accaaatcca atgccgtcga gaaaaattag
     1321 ccggaatgta aacggttaat tttaagcatt ggaaaataaa gaactccatt ttatagtctt
     1381 tgttgacttg tattaaatat aggtaaaatc aattcagcaa ctgttttcac ctgtgtcggt
     1441 actcacctgg tcttgccgga tattataagg cttcgttatc tcaccggtag tgtgcattgt
     1501 tagaataatc acaacctgat aagtttcttt cagtatgcct cgatgcctac atactgtcag
     1561 cttcattaat tttacctata agctaaaaag aaagttaatg taaaagaaca ctacaaaata
     1621 aataacctgc aaccaaaata caacgtaaat tgttagcatg atgtactgtg tatagcatcc
     1681 aaactttttc tttcctaata tcctatcttc aatgaacttt gcaactaaca actttgaaaa
     1741 taatactact aatttggtga tactgatttc taccattatg ttgttgctac tgcaaattta
     1801 atactatttt tcaattatgc aagtttttat ttataatgtt gtataaacct tattttattc
     1861 tctattcagg aggatttttc aatatctctg tctgaattac actcaattta tccatttttt
     1921 ctgtatcgtc catactgttg acgtaattta atatttcctc tgtgctccaa ttactgaagt
     1981 cgtcgggatc ggacggaggt aaactgatta aactacggta taacatacct tttatcattg
     2041 caagttgaga taaatctaaa tccctgcgac ggactacctt tgttttctcc cgatataccg
     2101 gctctaacct gcctggcagg aatactttgt catacgtgaa tgttgtttcc tccacaaatc
     2161 cattattcag taatgcattc agcctgttat ctaccaacga taactgccgg gtgtggttaa
     2221 tgagtgcggc cgctctacac gcctcctcgt acatctgccg tatgtaggca ttctttctca
     2281 accaatcaat ccatgtgagg taatttacat tgtacctgtc acacgcttca gttattgtca
     2341 cgcatcctgt accgaatacc tcgcaaatat gcattgccaa ccgttccatg tcctccctgt
     2401 tggtatcatt caattccacg atatcctcat tcagtatatc attccggagt tgaattattt
     2461 tcctcatatc caatttcggc ttgtcagctt tgtcagcctc cgaaatatcc acgattaggt
     2521 tactgtcgta caaagtttgt atcgttacat caaactccac tatttcctgc tttgttgact
     2581 tatcgattat cctgcatata aatggctttg tgaatatgtc gttacgttct ttcgtgtact
     2641 tcaggtaagt agcatccttg aaaatcttat ctattgcaaa tccgcaatcg atatacttgt
     2701 acagtgtacg agtggtaacg tccattacct ttgatagatt tttagatgca gtcatgtttt
     2761 tgcctctggg tacctgccgg tacagaatcc gggccacgaa tactattgca aacttgttgt
     2821 ccttttcagg cagagaaata acgtaccctt tcgacaatag ccaattatat gagagtaaat
     2881 cttctaactt aaaatcgttt acgctttcca ttttgtctgt ctttcacccg atgaatttca
     2941 cctcttgggg tataaggtat cgttttagtt ttcagttctt tataaattca aattctgtcg
     3001 ttcctccact atattgtgca atattcaaca cttgtctttt ttagcacaat gaaagcaatg
     3061 aaatcaactg attattttgc tcttgtggag taatgctctc gccttttcat ttttccatag
     3121 tgcatgatac ttttcgtgtt cctccggaac gtcaatctcc tgttcaccat ttcgtacttt
     3181 ccaatagttt gtagtaatga ggcaccgttt ctcataatct gtcatattat tgaaaatcgt
     3241 gtaatctttc ttttcttcag cttggttaaa caggtccatt aatcggtaaa ttctaatttg
     3301 taattgttat ctacgtgttt cttgccatac ttcctgttgt ttacgtgtat tggcatatcg
     3361 taacctaaat ggcacttttg gcaccatgct cttagccggt ccaatggtac tttctcgttt
     3421 gacttgtcgt gatctaaatg tgctatcgtc aataccactc ttgtaaatcc gtatttcttg
     3481 attgcctgtg tgaaagagta atcgtggttc tttaccctgt tgttaatcat ttcccattct
     3541 gctgaattag gatatgttac cctgccgtcc ggccaacgtt ttattattgc ttggtttgct
     3601 actccgcact cctcgcattt attctctgcc ctggcaagaa ctgcaggtct tatttctgtc
     3661 agccaattat caggatattc tttatagttt atcggcatac gttactgttt ttgctttatt
     3721 agtaaatcgt gttccttttc cattgccata ttgaattgac tgattttgtg gagcatggta
     3781 tctatacata tccgctctgc caacttatcg cacataccaa actgtaagtc aggtttaata
     3841 ttcttcagta aactttgtac gaataccaat tcaatcgttt ctgattgtag tccgtatgac
     3901 ttgaatacat cgtcaatatt ttcaatatta tcagagaact gttcctgcat tacatccatt
     3961 tagttttttc aatgaatttc tgtactactg cctgtacctg ctcctcgttc aatgatgcat
     4021 ctacataaat cactgttcta tcattcacaa ttaccggctt tcgtggtatg ttcctcggta
     4081 caaataccgg tttttctttt tctaaaaatc cgtcggccca ctttttctcc tgtttcatcc
     4141 gctcatcacg ttttctgata gcctcggctt tacgtttctc ctgctccaat atctttctgc
     4201 cggaatttag gcatgaaaaa aagtcaacct ttttgacttt cttctcaatt ttctttattt
     4261 cagacttcag aggacgtttc agcttcagtt tatttaactg tttccgtatt gtatctgcat
     4321 tcctatttaa taagattgcc aactcactat ttgttttatc gatataattc aacgtcagcg
     4381 tttctatgtc gctatcagtc caatgcttgt aatcccggtc ggttttttta gtatgtttca
     4441 ttagaatatg aatatttggt tagtataaat atacattgct aatttctcat ctgccataat
     4501 atactcctta aattcagagg taagcatacc taccgacgtc gcgcaatcac ctaatccctt
     4561 atacaaatga actactttta ccactttatc cgcggtaact tctttccgga ggcaatactc
     4621 ctcccaaaaa actattgtgg taatgactac tttggctctc tccttagtct gcaggaactt
     4681 gtcaaacttc tcgtcaacta acaggttggt attaattgca atgatcgcat tgtttaccac
     4741 tcccaggtcg gatttacaca aattcagaaa cgcctcattg tttttgaggt gttccgatac
     4801 atctttgggg tgcttaccga ttgtttcaca tgaaacagtc aatccgtttc ctatcttcaa
     4861 agatgaacga aaaagatttt cttcattcac tgataggctg aacttactca ttcaataatt
     4921 gctgaattgt aatggcaaaa tcttcagatg ttgtattttc caatgcctcc acatctacac
     4981 ctatatcttt ggctaattca aaaatcggtt taaatgtgct ttcatacacc tgattgttct
     5041 caatagtcac gaatacaata aatatcatca tttccattaa aactcgctca tacgactgat
     5101 tattgacgtt ctcattgaca agtttcataa tgtccaactc tataatttca ggactattca
     5161 ccggcattcc gtcatatttc cattttaaat tttccaacaa ttcagatacc tcattgaatg
     5221 caatgatatt ataaaatagc attttcagga ttgaaacagg cattttatcc aattcttttg
     5281 ctcctaacat aaaaccaacc tcctctttca gtttattttt aagggaaata cggccattct
     5341 ttattttaac gttttccata cgcttttgac taggcgtaac agtcgcttta accggttcat
     5401 tatgtattat tgtttccact tcattaactt caggcacttt ctcataaagt ttaattactg
     5461 tacctaacaa ctgaatattg tctgaactga agatgatacc gaataacttg gaatcgatgt
     5521 tatctacttc attttcagga atttgatgta cctctgtaag tgataccagg ttttcgtgct
     5581 cattctcatc cggaattaaa gacgtatcta tacggataac ggtattacct gcatccacta
     5641 actgtttctc taatgtagtc agctttgcag acgttttctt ccaaaaacat ttggtatcaa
     5701 aacacaaatc gtcgctctgc acgtcaccaa acaatgtcct gttggctcca gaacgtttca
     5761 tgcagttaat acaactgccg gcgcttttat tcaatctttt atcctcaatg tcaaaaggtg
     5821 cctttgtcag taagcataca aactcctttt caatgatacg tcgcatttct ttcactgaac
     5881 ggtatgcaaa ccggtgttct ccctcctttt cattgtatgg tacattgtac tttatacgtt
     5941 cgagaagttc caactgtttt gcgttttgca attttgcaaa ctgtaaacaa tggtggaggg
     6001 taatgtggct gtctttaaat aaacgcttta tctctgcatt caggctcttt aagtgtattc
     6061 ggtcatatac atactgctcc gatacaccaa tggttgatgc aatctgctct acggaggtta
     6121 ttttcgtcaa ctgcacaaac gtttcactct cgtcaatagg gtgtggttgc tctctctgca
     6181 cgttttctat cagctgtatc tctactaact tatcaggttt tacattctga ataattcggg
     6241 agtcaatttt ttcccaaccc agggacttcg cagccaacaa cctacgctca ccaaatacta
     6301 aatgatattt acctttgccg gtatctctta ccataatcgg atgcagaagt tgcaccgatt
     6361 tcattgattc tgctaattca atgattttct cctcgtcaat atgttttcga tagttcccat
     6421 tgtgtactat tttgtcaatc tcaatagatt cgacgttact gaatgattga atactttttt
     6481 ttagtgccat tactgtcgta ttttatagtt tgtaaaatgt agaataaacg tttgatgtag
     6541 gctaaatatg catctttggg tttcagataa caaatatggt aattattagc taaagttgca
     6601 tgattaaaat aaaatacgtt atactcaatc tgttgtgcca taatatcgta tttcttaatt
     6661 cttttcatgt aacgctcatc caaaccaaaa taatcgaaaa tacacgactg tactccatcc
     6721 tcaatgtcct tgaataatgg tagtaatagc tttatcggtt taatcatgtc aggcaggtaa
     6781 gcctcactac cgtcatgtat caatgcacat aaacgttcct ccggctctgc gtcataaggt
     6841 agcaaactct caacgagtat gcaatgctct gcaatgctaa agaactccgg tgtttgtcct
     6901 gaaaaatgtg gcttattggc taatcctttg catatatcca atacgttcac catgtcagga
     6961 cgtggattga taaaatcaaa cctcaaacct gaataggtag atatgcagtt gtcgtatgcg
     7021 gttacatctg ttgtcatgcg aacggatcta cttcagcgtt ttctaaatct ctcaatattt
     7081 tttctttggc ctcatccact acctctgcag gtttatttgt cagctgctca acacgaaatg
     7141 ctccggccaa tattttttta cagaagtctt ttgtatctgc agtaaaaaat tcctgttggt
     7201 tcatccattg cacgtattgg atatgtttac ttacttgatt accctgaaac ttaccaaatc
     7261 cgaatacgat atttcctacc tcatccaata agaacttacc ggctatatcc acctggtcct
     7321 tacttgttgt taaaaaattt tgtacttcgg tattattata tttaaccatg gactgcagga
     7381 ttacatctac tgcatataca tcatttaaag catcatgcgc tccgtcaata tctaaaccgg
     7441 tatgttgctt gtaaacgtta ttcagggtac gacggtacaa tgctttatcc atttggaaca
     7501 cgtcgataat atcttttggt acatcccaat caataccaca tcttttgaac tcctctttca
     7561 gtaatggaat gtcaaaattc ttaatattga agcctatcaa cgtaacatct accatgtact
     7621 gaaagatgtc gtttgctatt tcaggaaacg tcgatgcatc tttcaccatt tcatctgtta
     7681 tgccatgtac gtctgttgca tctaccggta taggtattcc gggattaatg aggatatttc
     7741 ttgtatctct cattccgata ctgtttgtgt aaaccaatgc aatctgtacg attctatcag
     7801 ttaatacgtt cgttcctgtt gtttcaaggt cgaaatttgt gtttggtgtt ggtactctgt
     7861 aactcatata aactttgttt tgttgcttag acaaaaatac ttaatttata caattataca
     7921 aactatctga gaaatatttt aaacaaaaaa atgcacctct ttcgaggtgc ataagaacta
     7981 aaaaaactta ataaataacc aaaaatttat aaatccattt tactaattct gcctttataa
     8041 taactaccag gattgaatga ttttgcttta tcgtttacta caataagaaa tacattattt
     8101 acctctacat aatccaaatt atcagtattt tttaaatgat aatggatagt ggaagcatta
     8161 atcttttcat tgcctgtact tgctctcaac tgctttacaa attctgcaat cgttacgcaa
     8221 tctgcaacta atatctgatg ctctgataaa tttaatttca tttctttgat tttgctttta
     8281 tgactgcata cgtcgttaca cctatcgcta ctccggttac tattgtaaag tttgaaaaaa
     8341 acaactttct tcttcttttc ctgttttcct ttttgagtgc tttattctcg ttctttaaaa
     8401 gtacaatttg tttatctttt tctgaagcta ttaatgataa attatctatt tgtgttgctt
     8461 gatctaaaat aatactgtca ttaatattat ttttttctac aactaatttt tcataagtca
     8521 atgaagtcaa aagtaaacta tcgtatgact ttgctttaat atcgcttttt ataagttcca
     8581 acgtactctg tttgtcaaaa cagaatttat cattaatttt taccggagga tttatttgag
     8641 aaatacctaa tgatgctttc gctaccaaaa gtatcaacat cagcaatctt ttggcttgta
     8701 gttttaataa ttccattctg tttttgtttt atgtaaacaa tttcttttgt aataacattt
     8761 ttcctccacg tcagcgtatc tatcttactt tgcaggagtt tgttctgttt tgtcaggcta
     8821 tcaattacca tttgcttttc agcatatatg gccttaataa taccacttaa ttctttcgtg
     8881 ttatcagctg aactctgaat gcgaaatacg atacctgtaa ttatcaatgt tgctgcgaat
     8941 attgcaatgt aaatccaatc acgctttaca aatgcaaaga tttctttttt tatttctgtt
     9001 ttcattttat acgtatttaa taattaaaat taatacagtt gcggtaattc cgtaccacaa
     9061 aaaatatctc gacaatgttt ccaactcaac cattacagta tttataatca acagtgattc
     9121 caaattgttc tctgcttcat tctttttctg caggtttgca taaacctttg ctaataagaa
     9181 ccggagtgaa ataaacacac ttggaaacca cgaaaaaatt agtgctacta taaacatata
     9241 ctattattta aagatgtcct cgtctgttac ctgttcttca gtcttttcct ctaccggagt
     9301 tgtcatgtta tcgaggttaa gttgtaactg ataatcttca gctttcaaat cctcaacgtg
     9361 caatacaacg tgagtgccag gctccatgta aattctctgc ttattagcaa agtcaaatgc
     9421 taaaatcgta tctacattac gccactcata tccctcacga tacttagatg caatgatcga
     9481 tatattcgtt tcggtatttt taacgatgtc agcaagacgt ttcgtttcag ctttcttcat
     9541 ttcctcttgc tcctctctca ttttttcgaa ctcacataac cggtctgcta attccaattt
     9601 ttcctccggt gtaaatgtgt atttgatttc tttcagttgg ttagaatggt attctgtcac
     9661 ttcattgcct accaccttaa attctaaggt aatgtcggga taacaatact ctctgatttg
     9721 gtcgaatgta acatcagacg gaatgatgta gtttggaaac ggctcttttg caaagtcgaa
     9781 tacgatttcc tttgagtaat tctctttggc attttctacc aactttgaga ttacctcgtc
     9841 taattcggta cctgctggtg ccattaagat ttttccattt gaaaataact tctgcacaaa
     9901 atgtttgttt attggtgatt aaataatatc ctctattgtt actaaaattt tctgttgttt
     9961 caacgtgtca acctgtgttt gtttcggtat gaactctacc acgattgcag gtttatcatc
    10021 tacgataatc ttgttgccat gcaatgcgtc gcctatgaat ttgaatgcac ctgcatagtt
    10081 atcccaatcc attaatttca ctgcaaacct gtggaatacg atacgcacct tacctgggtg
    10141 ccgggaaact gtctgtgagc gaattttcca ttgtatctgt tccaacatct ttgcacgttt
    10201 agtccaatgc atacggagca ggatattcat actcggaaca tcactatttt ttaaaatcac
    10261 tgttgttatt gccattgctc aaagttaatt aagtttatat agctatccaa attatcgttc
    10321 tgttttttta taacaaagta taaccgtaat cctgttatgt ggtacttgat tgctttccgg
    10381 attcatggac tacgtttata cgactgttat aagaaatgct acgatacatc tccaaataaa
    10441 ctttgtggtg gaaaagaatt attaaaaatt ttccctcctt tttcttcttc aaatttatca
    10501 gtttcgttac cccaaacatc ccatccttct gctcttgttc gtgcaaacat ttcaagtttt
    10561 ggaacatcgc caaacattcg ttcaatccgt tttctaaatt cgtttggctt tttactatgt
    10621 tctgttttat tttcttccaa gtatgatttc tccttaaaag atttcagtaa tttatgtgct
    10681 gttccttttg tagcaagtag gcatatttca cattgcttta cagtccattt acccataaaa
    10741 ccacgctttt tgttccaagt aaaagcaacg gttttgtatt tgaaccccca attatttatt
    10801 gcctttattg ctacttctaa atgagcatca gtagtccata caaacaaaac acaattatca
    10861 tcagcataat cggctacatt caattcaatt atttctttat cggacatagt aggatattgt
    10921 attgataatt cctcattcgt tccaaacttt tctcttatgt cttttcgcct atttattcct
    10981 tgcgaaaatc tccaagcagg gtcagcataa ataacattat attttttgcc atcgcacatt
    11041 tttaataatt cttttctttc cgtttccata ttaaatttat cgtttaatta accgcacttc
    11101 ttataacagc agttaggtgc aatgctaaac gacaccgtca cgataaaatt tcttattcaa
    11161 attatgacaa gcaatcatca catcttcata agcaccttta tatcttgtct tattttcatt
    11221 atgtgtgtct attacttccc atttgttatt tatgcaaatc ttttgaaacc ttcttttggt
    11281 gcttagactt ggcatttcta ctttttctgt tattatttca atcatatttt tgaatttctg
    11341 agaagcactg cacctaacaa ggtattgcca aaagcagggc attctcggtt aattaatcgt
    11401 ttgtacttct attgggtatt tgtgcaaggt tgaaactttt gtctttcaaa tccctgcctt
    11461 cggcaatacc cgaaccgtta tg
//
LOCUS       DAOOOI010000063        11261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1121_length_11261_cov_66.328485, whole genome shotgun
            sequence.
ACCESSION   DAOOOI010000063 DAOOOI010000000
VERSION     DAOOOI010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11261
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1121_length_11261_cov_66.328485"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..174)
                     /locus_tag="PKX59_08095"
     CDS             complement(7..174)
                     /locus_tag="PKX59_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90865.1"
                     /translation="MEHTNKIAVKIIIEEFDDYKNNPENQWHYDREYKMIVKALEEQE
                     KLNKHDSMIIL"
     gene            complement(180..902)
                     /locus_tag="PKX59_08100"
     CDS             complement(180..902)
                     /locus_tag="PKX59_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90866.1"
                     /translation="MQNLKHLLTEIRTTPKEKNLTNREQMQFDAFLEVFKSVAGHYGR
                     KGVFSIDDENREQIKYLLLYFSNNRAFNEITPNNVMKYKPDLEKGLILAGNVGSGKTN
                     LLRIFQEMRIPSNYFRIAYCDVITDDYRRLGMQSLFKYYNSNLERIQHDTSESIFLFD
                     DLGFEKKFKDYGTEVNCLMDIIIKRERLYNDYGTRSLFTTNLMLSEIESYYDNRTLSR
                     LCGMCNVIYLGNKSDSIDRRIN"
     gene            complement(838..2001)
                     /locus_tag="PKX59_08105"
     CDS             complement(838..2001)
                     /locus_tag="PKX59_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90867.1"
                     /translation="MSGYIKLSRKYFNNFLWNEKRKFSKAEAWLDLIQLVRYDATSNG
                     KKIVNGKLIEWKRGEFPASSRYLNERWNWSGPSQVKRFLDLLKNEDMIATRTEQGETV
                     ISLCNYDSYNTDWNDVGTVVRTVVKHQQNGDKTVEEQKQNETVIQSKKDNTVKNDEKG
                     NIPSVDGFNPPAEKILKEEYRKEFLKVMKFIFEQPILQKIPKTLTVDEYEKLREKFTF
                     EQIEEMLIAMANHKATNKSQTIYLTLLTYLKKNFSQGEVKELEPIFEKMYRDFIVKQE
                     PDIHAVKLTDFDRRSMRTIIGYLLENNVNANKKRNDNRDTSITEFTDKDGAVISWRYI
                     LTNWNKLDNFMQKRVKLTEIDNDLMKILTDLKNAKLKTPADGNKDNSQRKEFN"
     gene            complement(2005..2289)
                     /locus_tag="PKX59_08110"
     CDS             complement(2005..2289)
                     /locus_tag="PKX59_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90868.1"
                     /translation="MSRSVRQHRSEWFYYRLIASWTGHTAYEVYEIMTKLFLLTVDEE
                     GNLGIIRPTSLTTVEHNEYLEQIRCFMAGFEFYLPEPNEIPTEDYIIKII"
     gene            complement(2261..2971)
                     /locus_tag="PKX59_08115"
     CDS             complement(2261..2971)
                     /locus_tag="PKX59_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90869.1"
                     /translation="MKTLKPKYKITPSKLESFRYLIEEHYGTTEDFIAEIKGTKEWSR
                     KMNFGSAFHAVIEFGPEKYYDFDSGKYIVKESDFPEAVVMEQEQIDVAVRFRNNHSRM
                     VSECKHSFELDMGAFIAVFSLKVDSLEGDIVNENKTTDKAVSFEKYYKSIQWKCYILA
                     TGVQKVVYNVFKYKGNEKKGYEVDEPVDFTFFPYENLKNDVYSLCREYIDFCTRHDLL
                     DYVTVKPKANVEKCSTAS"
     gene            complement(2946..4292)
                     /locus_tag="PKX59_08120"
     CDS             complement(2946..4292)
                     /locus_tag="PKX59_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ90870.1"
                     /translation="MEQIVQEQQWNIVEETDALKIASILTVIFGDPGMGKTSLSMQSE
                     ESILLDFDDGLKRSTKRKRAVQMRTFAGALDFIRSEKFKELKPRTVILDTGGTLLDNY
                     IAHYVIENETANKKKGGGLSLQGYGALKEVFSQFINELKRMQIDIVIVCHTEQYKDGE
                     NLRFRPKMTGGSYDILMQVADLVGYMESKNEKRTLNFNPTDRHIGKNAPNFANFIIPH
                     AETIEFDNFLGRIIADAKEKMSKLDEEQQKALLLLDEKRKEVDEADTFEKLVALTETF
                     ADLKPAFQIQLSKLVEDKKTEFIKVMISGLTEPLQLDVMLNEIKTKYGDSNFYKRLLN
                     DRKTGLGFEFDKDNGVFVKPEVKDEPGAEVKEEDKQPVLNAVTEVPAEQTNAPAEEVK
                     EEDKQPVLNAVTEVPAEQTSAPAEEVKEEVKKSASKKKAEEPTTELFKNENAEAKV"
     gene            complement(4383..6413)
                     /locus_tag="PKX59_08125"
     CDS             complement(4383..6413)
                     /locus_tag="PKX59_08125"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOZ90871.1"
                     /translation="MQDFTELYNGLNAFRELFKQRILPETEVSISAKFLRGIAQKDYK
                     TWTIDEAIRLHNIIKPYGLKLKRVGFDISKIPVIQIQLSVVNRDNVVQPEQRRLIGTD
                     GNFFIITFPYRKELYDAVKNIPRARFMYDTKRWIVPLEFAKEVTHFANGYEFQIGERA
                     MNLINLQYESGENMELSYMTEYIELNIPLKKKLYPYQTVGGDYVRRNKRVVIADQMRL
                     GKTMQSIAGILVTNSFPCIVIVPKSLRLNWQDEWHDWTDRKAIVLNHKNVKKLPELIR
                     QGMADVVITNYDGVQSFLIDDLKEVNITTGPNAGTKYHNAKGNDLCNLFNSAIIDEAH
                     HCRNKQTIRYKCVREVFKGKEVRICLTGSPIVKGPKDLAALFELIGRIEEFGGYDKFI
                     KKYKKIDAKTLDDKGKQLQELQELNTKMRSLCFIRRERHQVNEQISEKFRKVIRVDLD
                     NQSEYDHAWLSLQSYMAERAFTTSQISKALQAEMLVKMGLLKQISARGKSASVKEFVS
                     ELIDNGEKVIIGCWYNDTVQYFKDAFREYGAVTICGRIDGHDMKPEEIHENKKKFQTD
                     DNTKVIIITYGKGGEGHTLSKASSLVMVELGWTYKDQAQMEDRGIAVNKTEDFNVYVF
                     LGKDTLDEDTYEIIDKRRVIEKEAVGGSEHIENSEESVISAMKDKILKKISK"
     gene            complement(6604..7155)
                     /locus_tag="PKX59_08130"
     CDS             complement(6604..7155)
                     /locus_tag="PKX59_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90872.1"
                     /translation="MTNSERYTVIERIYANGGIFNIDVTTGPVLTSEGIVIDDDVQLF
                     LIVVAVAAGINDADTNLLSKMSAKFATLTATLPTSVYIDAKGCNSNTNPAVADAGIFY
                     LSPQIYDNGVDPPQTYNFNVLVVDFLNNHTGITRLTSGHELFPYFEEYRANLQLEVLE
                     NVIPINYIIYHEDTNEYLIVEIL"
     gene            complement(7157..9349)
                     /locus_tag="PKX59_08135"
     CDS             complement(7157..9349)
                     /locus_tag="PKX59_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90873.1"
                     /translation="MAQKILWNWQDDDSTKHLNQRTLGIVEPGLYRGFDIDLSDVSNI
                     KLVQTVTGATIVLTDSPVFTTEQRGVVVTKQGVVIHEDDVVVLPFTPNTSGNPRIDVV
                     IMEHEYVDVMDGSNVVYSVLLGTPAGTPVAPTLTSPKTQTILGYLYVPNGAEYMDAPG
                     VVYTRAVTPNFAGDGSIAHQYLLNIFLGIIGMVRNCVLDNVGNDDPVYYFSLNLNSAP
                     AGAVNKDANNFILDYGYTGGIQNENVRLRYVGPDENLGGKEVTIYTKQELRIDFGYDI
                     LPSDGDATYCYIPANSVFVLKSVSNTSPVVTDNNKWVLVNAQRASENRSNNFTASNKF
                     SGTVTENTKQITIGDDGKSSSFTATDGNILKITSMGSPSTVGATSVTGFKALPVDTII
                     KIDLSDAGITLDHVIFKNNDFVASGGNLPIYTTNSGDLVVNKGGCVWLKAEASAYRVV
                     AVQGEKKHYDGYFVLDNSAFNALSKTITVNNRNNCFSILVPTAGSLENINVVDDYGAT
                     VDVRPGTIINIRFKHIGGSIASSGGVLTAVPFLISLENTSNIRSNVSDFGYISSLGKS
                     LSIIDDQIYGESGDVITFRKTDTVWEIVSANRDAYYAHKLNDKKIDKGVTWLTADFTH
                     SSISPREASRPAMRLCKNDLGMMIVEFSFGVSADIAAGTIIGRFVGVLNGYVWSKAVP
                     VTYKQNGVGFSIGHLSFTIGPTFQDMQIIALTAIPNTYGIYQPILLIP"
     gene            complement(9351..9731)
                     /locus_tag="PKX59_08140"
     CDS             complement(9351..9731)
                     /locus_tag="PKX59_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90874.1"
                     /translation="METANNNEILQKTFEYIVGTDDIAFAIAGVFFVVIGCILSVTIR
                     ALRVGVKMPGTPNKFSSRYMFKDKIAELLLGFVLACICMRLFNDWIGAQATMGLGFVL
                     GFCNYRLANLIPKVFKFLIEDKLK"
     gene            complement(9735..10130)
                     /locus_tag="PKX59_08145"
     CDS             complement(9735..10130)
                     /locus_tag="PKX59_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90875.1"
                     /translation="MSPLIIALITIFFSSPILVTVVNFFMYRRKNTADVDHIIMQNME
                     ILVNSYKDNKLLDEQIKSELRTEIAEHKINLRTIRERQEMYLKNSTDALSLLANTQKE
                     LVVIKENYQKCMKEHEFARKEIETLKEKS"
     gene            complement(10151..10702)
                     /locus_tag="PKX59_08150"
     CDS             complement(10151..10702)
                     /locus_tag="PKX59_08150"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOZ90876.1"
                     /translation="MAIFVSAGHNPNGIKKDPGAVGNGFREADLTVEFRNLVLGSLLK
                     RGFKGIADDDSETLGQYLKRIITGNGSVVLEFHFDAAINPTATGCTSLIGSDADKNDK
                     HFGNELVEATAQILGIKNRGCISESESHRGRLGLMRERGIVALLEICFITNASDLKKY
                     QDNKFTLADKIAEIIIKYENLIN"
     gene            complement(10981..>11261)
                     /locus_tag="PKX59_08155"
     CDS             complement(10981..>11261)
                     /locus_tag="PKX59_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90877.1"
                     /translation="YEYWHKHLSSKNKYEYTGKVKVWLMKNLLVMFTDGWHKYQFVLT
                     ICLLVSGFFVGFISGKITPYYSFMLLSVYFVRTATFHLLYTSKKYRKL"
BASE COUNT         3176 a         2500 c         1729 g         3856 t
ORIGIN      
        1 acggcgttat aaaattatca tactatcatg tttattcaac ttttcctgtt cttccaatgc
       61 tttcactatc atcttgtatt ctctgtcata atgccattga ttttcaggat tatttttgta
      121 atcgtcaaat tcttctatta taatttttac tgcaattttg tttgtgtgtt ccatactttt
      181 taatttatac gtctatcaat actgtcagat ttattgccta agtagataac attgcacatt
      241 ccgcataacc tcgataatgt acggttgtcg taatagcttt caatctcact caacattaga
      301 tttgtcgtaa ataatgaacg tgtaccatag tcattgtata atcgttcacg ctttataatt
      361 atatccatga ggcagtttac ctcggtgccg taatccttga acttcttttc aaatcccaaa
      421 tcgtcaaaca ggaatatgct ctctgaagta tcatgctgaa tacgctccaa atttgaattg
      481 taatatttaa acaatgattg catacccaac cgtcggtaat cgtccgttat cacgtcgcaa
      541 tacgctatac ggaagtaatt ggacggtatt ctcatttctt gaaatatccg gagtagattg
      601 gttttaccac taccgacgtt accggccaaa atcaaacctt tctctaaatc aggcttatac
      661 ttcatcacat tgtttggcgt gatctcattg aatgctctgt tgttgctgaa atacagtaac
      721 aggtatttta tctgttctct gttctcatca tctatactga atactccttt ccggccatag
      781 tgcccggcca ccgatttgaa tacttcgagg aatgcatcaa actgcatttg ttccctgtta
      841 gttaaattct tttctttggg agttgtcctt atttccgtca gcaggtgttt taagttttgc
      901 attttttaaa tcggttaata ttttcattaa atcgttatct atctctgtca acttcacacg
      961 tttctgcatg aaattatcta acttattcca attcgtcagt atatacctcc acgatatgac
     1021 ggctccgtcc ttatcggtaa attctgtaat ggaggtatct ctattgtcgt ttcttttttt
     1081 gttggcattc acattgtttt cgagcaaata accaatgatt gtacgcatag aacgtctatc
     1141 aaagtcggtt aactttactg catgaatatc cggttcctgt ttcacaatga aatcacgata
     1201 catcttctcg aatatcggtt ccaactcttt cacttcaccc tggctaaaat tctttttcag
     1261 gtacgtcaat aatgtcaggt aaatcgtttg cgatttgttt gttgctttgt gatttgccat
     1321 tgctatcagc atttcctcta tctgttcaaa ggtaaatttt tctctcaatt tttcatactc
     1381 atctaccgtc agcgtttttg gtatcttctg cagtattggt tgttcgaata tgaatttcat
     1441 cacttttaaa aattcttttc gatattcttc tttcaaaatt ttttctgcag ggggattaaa
     1501 tccgtcgaca gacggaatgt ttcccttttc atcattctta actgtattat ctttcttaga
     1561 ctgtattaca gtttcgttct gtttctgttc ctctactgtt ttatctccgt tctgttggtg
     1621 tttcactact gttctaacta ctgttccaac gtcgttccaa tcagtattat aactatcgta
     1681 attacaaaga cttataactg tttcaccctg ttccgttctt gttgcaatca tgtcctcatt
     1741 cttcagtaaa tctaagaaac gtttaacctg tgacggtcca ctccaattcc accgctcatt
     1801 taggtatctc gacgatgcag gaaactcacc tcttttccat tcaatcagct ttccatttac
     1861 tattttttta ccattgcttg ttgcgtcata tctcactaat tgtatcaaat ccaaccatgc
     1921 ctcggccttt gagaatttac gtttctcatt ccataggaaa ttattaaaat actttcgtga
     1981 caattttata taaccgctca aaacttagat aattttaatg atataatctt cggtaggtat
     2041 ttcattaggt tcaggcagat agaactcaaa tccggccata aaacaacgga tttgctctaa
     2101 atattcattg tgctcaactg tcgttaatga tgttggccga atgatgccta aattaccctc
     2161 ctcatctact gtgaggagga ataatttagt cattatctca tagacttcat aggcagtatg
     2221 tcctgtccat gaggctatca acctgtaata aaaccactcg ctacgatgct gtcgaacact
     2281 tctcgacatt cgctttcggt tttactgtaa cgtaatctaa caaatcatgt cgtgtgcaaa
     2341 aatcaatgta ttccctgcaa agtgaataca cgtcattctt caggttctca tacggaaaga
     2401 atgtaaaatc taccggctca tccacctcat accctttttt ctcatttcct ttgtacttga
     2461 atacattgta aaccactttc tgcacccctg ttgctaagat gtagcatttc cattggattg
     2521 acttatagta tttctcgaaa ctaacggcct tatcggtcgt cttattctcg ttcactatgt
     2581 caccctccaa actatctact ttcagcgaga atacggcaat aaatgcaccc atatccaatt
     2641 caaaggaatg cttacattca gataccatac gtgaatgatt gttcctgaat ctgactgcaa
     2701 catcaatctg ttcctgttcc atgactacgg cctccggaaa gtcactttcc ttgacaatgt
     2761 atttaccact atcaaagtca taatactttt cagggccgaa ctcaattact gcatgaaagg
     2821 cgctaccaaa attcatcttt cgactccact ctttagtacc ttttatttca gcaatgaaat
     2881 cctccgtcgt gccgtaatgc tcctcgatga gataccggaa actttccaac tttgacggag
     2941 taattttata ctttggcttc agcgttttca tttttgaata gttcagttgt tggttcttca
     3001 gctttctttt ttgaggcact tttcttcact tcctctttca cttcctctgc aggagcactc
     3061 gtctgttcgg caggcacttc ggtaactgca ttaagcaccg gctgcttatc ttcctctttc
     3121 acttcctctg caggagcatt cgtctgttcg gcaggtactt cggtaactgc attaagcacc
     3181 ggctgcttat cttcctcttt cacttctgca cctggttcgt ctttaacttc aggttttaca
     3241 aacacaccat tatctttgtc aaactcaaaa cccaaccctg ttttacggtc attaagcagt
     3301 cttttgtaga agttactatc accatatttt gtcttaatct cattcagcat cacatctaac
     3361 tgcaacggtt ctgttaaccc tgaaatcatt acttttataa actcagtctt tttatcctca
     3421 acaagtttag ataactgaat ttggaatgca ggtttcaaat ctgcaaatgt ttctgttaac
     3481 gctaccaact tttcgaatgt atcggcttca tcaacttctt tacgtttctc atccaaaagt
     3541 aataacgctt tttgctgctc ctcatccaat ttggacattt tttctttggc atcagcaatg
     3601 atacggccta aaaaattgtc aaactcaata gtttctgcat gcggaatgat gaaattagca
     3661 aagtttggtg catttttacc gatgtgacgg tcagtaggat tgaaattcag tgtacgtttc
     3721 tcattcttac tttccatgta accaaccaaa tcagctacct gcataagaat atcgtaacta
     3781 ccacctgtca ttttcggacg gaaacgcaga ttttcaccgt ctttgtactg ttccgtatgg
     3841 catacgataa caatgtcaat ctgcattcgt ttcaattcat taataaactg actgaatacc
     3901 tctttcaatg caccataacc ctgaagtgat aagcctccac ctttcttttt gttggcagtt
     3961 tcattttcga ttacatagtg tgcaatgtag ttgtccaaca acgtaccacc tgtatcgaga
     4021 attacggtac gtggtttcaa ttccttaaat ttctccgaac ggataaaatc caatgctcct
     4081 gcaaaagtac gcatttgcac tgcacgttta cgttttgttg aacgctttaa tccgtcgtca
     4141 aagtccaata aaattgattc ttcactctgc attgaaagtg atgtctttcc catgccggga
     4201 tctccgaaga ttactgttaa aatagatgct atcttcaacg catctgtttc ctcaacaatg
     4261 ttccattgtt gttcttgaac gatctgttcc ataaataaaa aagtttattg gtgaaaaaat
     4321 aaaaagtttc ataagaagtt gttgctaatg caaatctaat taatttatac aactatacaa
     4381 atctatttcg atattttttt cagaatttta tctttcatag ctgaaattac agattcttca
     4441 ctgttctcaa tgtgctctga accaccgacg gcctctttct caatcacacg tcgtttatca
     4501 attatctcat acgtgtcctc atccaatgta tctttaccga gaaatacata cacgttaaaa
     4561 tcttcagttt tattaacggc aatgcctctg tcctccattt gtgcctggtc tttgtatgtc
     4621 caacctaatt caaccataac caaacttgat gccttgctca atgtatgtcc ctcaccaccc
     4681 tttccataag taatgataat aactttcgta ttatcgtctg tctgaaactt tttcttgttc
     4741 tcatggattt cttcaggttt catatcatgg ccgtcgattc tgccgcagat agtaacggct
     4801 ccatactccc ggaaagcatc tttgaaatac tgaacggtat cattgtacca acaaccgata
     4861 atcactttct ctccgttatc tatcaattca gatacaaact ccttgacgga tgctgattta
     4921 cctctcgcag aaatttgctt cagcaatccc attttaacca acatttcagc ctgtaatgct
     4981 ttcgatatct gtgaagtggt gaatgctctc tctgccatgt atgactgaag tgataaccaa
     5041 gcatggtcgt actcgctctg attatccaaa tccactcgta tcacctttct gaacttctcc
     5101 gatatctgct cattaacctg gtgccgttcc cgacggatga aacacaatga acgcattttt
     5161 gtattcaact cctgaagttc ctgcagttgc ttacctttgt cgtctaatgt ttttgcatct
     5221 atttttttgt acttcttaat gaatttgtca tatcctccga actcctcaat acggcctatc
     5281 agttcaaaga gtgcggccaa atctttcggg cctttcacta tcggagaacc ggttaagcat
     5341 atacgaacct ctttaccttt gaatacttca cgcacacact tgtaccgtat cgtctgcttg
     5401 ttcctgcaat gatgtgcctc gtcaatgatt gctgaattaa aaagattgca taaatcattc
     5461 ccttttgcat tgtgatattt cgttcctgca ttcgggccgg tagttatgtt gacttctttc
     5521 aaatcatcta tcaggaatga ctgtacgccg tcgtaattcg taataacgac atctgccata
     5581 ccctgacgta tcagttccgg caatttcttt acgtttttat ggtttaatac aatggctttt
     5641 ctatctgtcc aatcatgcca ctcatcctgc caattcagcc tcaaagattt cggaacgata
     5701 actatgcagg gaaatgagtt tgtaaccaaa ataccggcta ttgattgcat ggttttacct
     5761 agtcgcattt ggtcggcaat aaccacacgc ttattccgtc gcacatagtc accaccaacg
     5821 gtttgatacg gatatagttt tttctttagt ggaatattca actctatgta ctccgtcatg
     5881 tatgacaact ccatattctc accgctctcg tactgcagat tgattaagtt catggctctt
     5941 tcacctatct gaaactcata tccattggca aaatgtgtta cctctttcgc aaattccaac
     6001 ggaactatcc accgctttgt atcgtacatg aacctggctc tcggtatatt tttgactgca
     6061 tcgtacaatt ctttacggta cggaaacgta atgataaaga aattaccgtc ggtaccgata
     6121 agcctcctct gctccggctg aaccacgtta tccctgttca ctactgataa ctgtatctgt
     6181 attacaggaa ttttgctaat atcaaaccct acacgtttca acttcaggcc gtatggttta
     6241 atgatattgt gaagccgtat cgcctcgtca atagtccatg ttttataatc tttttgcgct
     6301 ataccacgca gaaattttgc actgatacta acttcagttt ccggtaaaat tctttgcttg
     6361 aataactccc taaacgcatt caggccgtta tataattcgg tgaaatcttg catatacttt
     6421 attgttgcta cacaaaaata gtaatttata cggttataca aaataaaaat gcatagaatt
     6481 aatctatgca ttttcaccgg tgccgggggg gaatcatacg aggtaattta gcaacaacaa
     6541 agtttgaaac caatagcgac cggctgactg taaaagtaac gctttttttt caatcgtgca
     6601 actttacagt atttctacta ttaaatattc gttagtatct tcgtgataaa tgatataatt
     6661 tatcggaatt acgttttcta aaacttccaa ctgcaggttt gctctgtatt cctcaaagta
     6721 cggaaacaac tcgtgaccgc ttgttaatct tgttattcct gtgtgattgt tcagaaaatc
     6781 tactaccaac acattgaaat tataagtctg tggaggatcg acgccattat cgtaaatctg
     6841 tggacttagg tagaatatcc ctgcatctgc aactgccgga ttggtattgc tattgcaacc
     6901 ttttgcatct atgtaaacag aagttggcaa tgttgcagtt aaagtagcaa actttgcact
     6961 cattttcgac aataaatttg tatctgcatc attaataccg gctgccaccg caacaacaat
     7021 taaaaataac tgaacgtcgt cgtctattac aattccctct gaagtcaata ccgggcctgt
     7081 tgtcacgtcg atattaaaga tacctccatt cgcatagatt ctttcaatta ccgtatatct
     7141 ttctgaattt gtcatattat ggaattaaaa gtattggttg ataaattccg tatgtattcg
     7201 ggattgccgt aagtgcaatt atctgcatat cctgaaatgt tggtcctatt gtaaaagata
     7261 aatgacctat tgaaaatcct actccatttt gtttataagt aaccggtact gccttactcc
     7321 aaacataacc atttaataca ccaacaaaac gtccaataat tgtacctgct gcgatatctg
     7381 ctgaaactcc aaatgaaaac tcaacaatca tcattcctaa atcgttttta cataatcgca
     7441 tagccggacg tgaagcctct ctcggactta tagatgaatg cgtgaagtct gcagttagcc
     7501 acgttactcc tttgtcaatt ttcttatcgt tcaacttgtg agcatagtac gcatcacgat
     7561 ttgcagatac tatttcccaa acggtatctg tctttctgaa cgtaatcaca tcaccgcttt
     7621 ctccgtaaat ttggtcgtct ataattgaca acgatttacc caaactacta atataaccaa
     7681 agtcgctcac attggaacgg atatttgatg tgttttcgag tgaaataagg aacggaactg
     7741 cagtcaatac gcctccacta cttgcaatag aacctcctat atgcttgaaa cgtatattga
     7801 taattgtacc tgggcgaaca tctaccgtcg caccataatc atctaccacg ttaatattct
     7861 ccaaactacc tgcagttggt actaaaatac taaaacagtt atttctatta tttacagtaa
     7921 ttgttttcga taatgcattg aatgcagagt tatctaatac aaaataaccg tcgtaatgtt
     7981 tcttctctcc ctgaacggcc accacacgat atgctgaagc ctccgctttc aaccaaacac
     8041 aaccaccttt gtttactacc aaatcacctg aatttgttgt gtaaattggc agattgcctc
     8101 cacttgctac aaagtcatta tttttgaaaa ttacatggtc caacgtaata cctgcatccg
     8161 acaaatcaat tttgataatc gtatctaccg gcaatgcctt aaaaccggtt acagatgtgg
     8221 ctcctactgt gctcggagaa cccatagatg taatcttcag gatatttccg tcagttgcag
     8281 taaatgaact gcttttaccg tcgtcaccaa tcgtgatctg ttttgtattt tctgtaacgg
     8341 tacctgaaaa cttattcgat gcagtaaaat tgttgctcct gttttcagat gctctttgtg
     8401 cattcaccaa tacccactta ttgttgtctg taactaccgg agaggtattc gatacgcttt
     8461 taagtacaaa aacgctattt gcaggaatgt agcaatacgt cgcatcaccg tcgctcggca
     8521 atatgtcgta accaaaatca atacgcaact cctgttttgt ataaatcgtc acttccttac
     8581 cacctaaatt ctcatctggg ccaacgtaac gcaaccttac attctcattc tgtattccac
     8641 ctgtgtaacc ataatcgaga atgaaattat ttgcatcctt gtttacggca ccggccggcg
     8701 cactgtttaa gttcaaactg aaataatata caggatcgtc gttaccaaca ttatccaata
     8761 cgcaattacg aaccattccg ataataccta aaaagatatt cagcaaatat tgatgtgcta
     8821 tactgccgtc acctgcaaag ttcggagtta ctgctctcgt atatactact cccggagcat
     8881 ccatgtattc tgcaccgttt ggaacgtaca gataccctaa aattgtctgt gttttgggac
     8941 ttgtcaacgt cggagcaacc ggagttcctg caggagtacc caacaatact gaatatacca
     9001 cgtttgaacc gtccataacg tccacgtatt catgctccat tatcacaaca tctattcgag
     9061 gatttccgct cgtatttgga gtgaatggca acacaacaac atcgtcctca tgtatcacta
     9121 ctccctgctt tgtaaccact actcctctct gctctgtggt gaataccgga gaatctgtaa
     9181 gcacaatagt tgcacctgtt acagtctgaa ccaattttat atttgaaaca tctgaaaggt
     9241 ctatatcaaa accacgatac aaccctggct ctactatacc taatgtacgc tgattaagat
     9301 gtttggttga atcatcgtct tgccaattcc ataaaatctt ttgtgccatt ttattttaat
     9361 ttatcttcaa ttaaaaactt aaatactttc ggtattagat tagccaaccg gtagttgcag
     9421 aaacctaata cgaaacctaa acccattgtg gcctgtgctc caatccaatc attgaataac
     9481 ctcatacaga tacatgctaa tacaaatcct aataataatt ctgcaatttt atctttaaac
     9541 atatacctgc tgctgaattt attcggagta cctggcatct taacacctac tctcaatgct
     9601 ctaattgtta ctgaaagaat acaacctata acaacaaaaa atacacctgc tattgcaaat
     9661 gcaatatcgt ctgtacctac tatgtattca aacgtttttt gtaaaatttc attattattt
     9721 gctgtttcca taacttagga cttttctttt aatgtttcaa tttcttttct tgcaaactca
     9781 tgctctttca tacatttttg gtagttttct ttaataacta ccaactcctt ttgcgtattc
     9841 gcaagtaatg aaagtgcatc agtagagttc ttcaggtaca tttcctgtcg ttctctaatc
     9901 gttcttagat tgattttatg ttctgcaatc tcggtacgca attcggactt tatttgctca
     9961 tcgagcaatt tattatcctt gtaagagtta accaatattt ccatgttctg catgataata
    10021 tggtcaacgt cagctgtgtt cttccgtcgg tacataaaga aatttacgac ggtaactaaa
    10081 ataggactac taaaaaaaat agtaatcaat gcaatgatta aagggctcat gtttttaata
    10141 tctattacca ttaattaata agattttcgt acttaataat tatttcagca atcttgtcag
    10201 caagtgtaaa cttgttgtcc tgatacttct tcaaatcgga ggcattggtg ataaaacata
    10261 tttctaataa tgcaacaata cctctctctc tcatcaatcc taaacgacct ctgtgtgatt
    10321 cgctttcaga aatacaacca cgatttttaa tacctaagat ttgtgcagtc gcctccacaa
    10381 gttcgttgcc aaaatgctta tcatttttat ctgcatcact tccaatcaat gacgtgcaac
    10441 ctgtggctgt cggatttatt gcagcgtcga aatgaaactc taaaacgacc gaaccgttac
    10501 cggtaataat acgcttcaga tattgtccta acgtttcact atcatcgtct gcaatacctt
    10561 taaaacctct ctttaaaagg ctccctaaaa caagattacg gaactcaacg gttaagtcag
    10621 cttcacgaaa accattgcca acggctcccg gatctttctt tattccgttc ggattatgtc
    10681 ctgctgaaac aaatatcgcc atattccatt ttttataagt ggagaataaa ttatcaattc
    10741 aatataaata aaaagtgcca ctttcaaaag tggcaactaa ataaataata agattgaggt
    10801 ttattattat gcttctaaag gactatatag tatttgtaaa aaatagtatt gctaatacta
    10861 ttcctgttaa tccgaataaa atcataaaca cctgtaaacc tgttttgatt ttaagtttct
    10921 ttgcaaagaa agaaacaatt actaacacta atgagattag taaaaagaca attcctccaa
    10981 tcataatttt ctgtattttt tagatgtgta taatagatga aatgttgccg ttcttacaaa
    11041 ataaacagac agcaacatga atgaatagta aggtgtaatt ttacctgaaa taaatccaac
    11101 gaagaaacct gaaacgagca aacaaatagt aagaacgaat tggtatttat gccaaccatc
    11161 tgtaaacatt accagcaaat ttttcattaa ccataccttt actttgcctg tgtattcgta
    11221 cttattttta ctgcttaaat gtttgtgcca atattcgtat g
//
LOCUS       DAOOOI010000064        11181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1131_length_11181_cov_27.754898, whole genome shotgun
            sequence.
ACCESSION   DAOOOI010000064 DAOOOI010000000
VERSION     DAOOOI010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11181
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1131_length_11181_cov_27.754898"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PKX59_08160"
     CDS             complement(<1..232)
                     /locus_tag="PKX59_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOZ90878.1"
                     /translation="MKRFRSLFGLKIMVVMVLLFSSFKAPDFTLKLAKLKYAGGGNWY
                     VSPTALHNLAVFCNQNINTNISPEEAVVEPGSP"
     gene            331..3249
                     /locus_tag="PKX59_08165"
     CDS             331..3249
                     /locus_tag="PKX59_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90879.1"
                     /translation="MTLRILLVAIFAPLMLNAAHDHNGFDRNEDPNLKVFVKDRKRLP
                     DVNRQESLAQQKSWQQFSEQNKGWSVVFNEETGLPHNAFGKPISVGLPLDAQSTAWSF
                     ISTKLNSFGLPLNDLYFRNTAQNKKYFYVNYIQRYNGLEVLWSKVQIKITKDGKVNQF
                     ICDAYKDITVNINPTLSTQAASAAAINGLNLTLNGTTVNSDLKVLPVPDAAKNIYHLV
                     YEVTVKATDAENFPREYYTLVDAHNGEVLYRHNKINYFAANTDVNVTATVYPTQPYLP
                     TAVEPLANMNVTISGVPFLTDANGYLGLPNTSPVTATFKLEGSWANVKTGATTPQFTA
                     TLNPGANNITFSSANIKELSAYNSVNEIHDYYVTKMMGSGAETVMDIQMQTIVDVSGN
                     CNAFYNGDLNFYAAGGQCNATSIINDVVYHEYGHGINYDLYSYYGGIFSNGALGEGYA
                     DTWANGLTEDPVLGIGFYSGNATGFVRRYDQGRKVYPDDIMGEVHADGEIIAGAWWDV
                     GLNFNSVQQRMDLYVQTFAATIDYPDGQEGQLYTDILVEALTIDDNDGDLSNGTPNYC
                     AISSAFGIHGINMFVVGKLITHNDVLSAPGQQNITLNASGPASFGNGTGNSVNGYYSL
                     NHSATWNPISFTYNGTIFQGSIPGQPNGTIVSYYLDATDQCGTHFSLTPPNVTDSNPN
                     LPYYVLVGYNLVASDYFDTGNSQGWTLGMPGDNATTGQWIIASPVGSILNPGGSPPVY
                     VQPPIDHTNTSNNLCAVTGNAAPNDGAGTNDIDDGKTTLLSPNFDLSAYSNPAISYWR
                     WYTNDQGATPGTDYWQVSVSNDGGTTFVPIENCIVSDHSWRRFAFRVADYVQPTANMR
                     LKFIGEDANDGSLVEGLLDDLEIWSAEPVGISETPMLSWYAYPNPANEQLNFGWKGNG
                     TTVDVKLFNTVGQIVYQNQFSGNTVSESVSLKGMAEGIYTLQMSGEKLFKSQKISVVH
                     "
     gene            complement(3318..3770)
                     /locus_tag="PKX59_08170"
     CDS             complement(3318..3770)
                     /locus_tag="PKX59_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90880.1"
                     /translation="MNYMLNRMNCCALVLIVAMAGSCKTNKNTTAETPKQEPAATNPR
                     VDLPDSMYRVIVSFISIGAGTDGAAKEKLEQVVQQYQTEVGMPVAHEEIAWGREGEVD
                     YCFRLLGQDKKSKDSFVQHLRDTFKGNSLVQITENTPALHQARPSFNK"
     gene            complement(3754..5247)
                     /locus_tag="PKX59_08175"
     CDS             complement(3754..5247)
                     /locus_tag="PKX59_08175"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HOZ90881.1"
                     /translation="MSELYQQYVTHLQKIADINYSSAVLQWDQEVYMPVKGAHYRAQQ
                     LSTLAGYAHELITDKRYEEIIEKLHDDTSKLSPVQCANVLESYRIFKQQKKYTTEFVV
                     ALNQCISKSFNAWQRAKQENNFKLFEPHLQELIKFKRQESELLGYEQHPYDAHLNLYE
                     TGLKTSFLETIFNQVRKKLVPYIQQLVQKPQPDDNFLFKSYNKDKQWHFGLDVLKQMH
                     FDFESGRQDISTHPFTTSFSANDVRVTTRISEHDLFEMIGSCIHEGGHALYEQGLPAD
                     DYGLPSGEYLSLSIHESQSRLWENHVGKSQYFWNHNFKTLRNIFPEQLHDVSERSFYK
                     AINRIKTSLIRTNADELTYHLHVLIRFEIEKDILSGALQAAQLPEVWNEKYKSYLNID
                     VPSDAKGVLQDVHWSHGSFGYFPTYSLGSFYASQFFHQANKEIPALMHHIQNGDTMPL
                     LMWLRSNIHRHGRTILADELCKNVTGEVLNFNYFFNYAKTKYDELYA"
     gene            complement(5247..5627)
                     /locus_tag="PKX59_08180"
     CDS             complement(5247..5627)
                     /locus_tag="PKX59_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008488040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HOZ90882.1"
                     /translation="MNKKILIFAALSGLCAVALGAFGAHKLKELLSPQMLSAFETGVR
                     YQFYHTLALLFVALAANENKMLNRAATFFMTGIILFSGSIYGLALSSISGNTWIWLGP
                     VTPIGGLCFMTGWIFLIIYAFKKQ"
     gene            complement(5627..6163)
                     /locus_tag="PKX59_08185"
     CDS             complement(5627..6163)
                     /locus_tag="PKX59_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90883.1"
                     /translation="MEELGIGSRIRHPEFGIGVVINVKPRTYITVFTERGKIEVAKTY
                     QNLEVIDAQPPVGDPVSFADMERVFTNIIKKYSDVQETVPLHGRWIGGKVVLHPNNPS
                     QKPKEIPMDVFFNKIIMIRDRLRVMEQRINAHDKLTEEDKINLQQYLTRIYGSLTTFN
                     VLFKDEEDVFVGEKGNKE"
     gene            complement(6183..6515)
                     /locus_tag="PKX59_08190"
     CDS             complement(6183..6515)
                     /locus_tag="PKX59_08190"
                     /inference="COORDINATES: protein motif:HMM:NF027575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4907 domain-containing protein"
                     /protein_id="HOZ90884.1"
                     /translation="MRIIGFIFSILFIVSCTNKPAEVKTPAPLINNVTVQTYANDSTL
                     GGYGYDILVDGKIMVHQPNIPAVMGNRGFATEEDASKTATLVSFKLKNNIMPPSVTLE
                     ELDSIGIK"
     gene            complement(6549..8165)
                     /locus_tag="PKX59_08195"
     CDS             complement(6549..8165)
                     /locus_tag="PKX59_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90885.1"
                     /translation="MLFILLSTVNAEGQMWQWAKSDGSLNNDAAISVSTDYAGNVFVA
                     GIFRDSIFSGANKLYSYGLDDIYVVKYSPNGNILWTKCFGGTGNDGYGLYIKADSIGN
                     CYVTGAYSYSAQFDLITLNSGFVNNMFLVRMNPTGYILFANDFQGTNGSVGYALEVDR
                     NQNCYLTGYFYDQLFAAPNTVYSNGSHDIFLAKIDGLGNVLWSRRAGGSSYDSGGGVA
                     LDDFGNIYITGYFSSTADFDIATLISSGGDDAFVAKYDNSGNFKWVSRTFGSGVYDIG
                     VKIRIQQDKICVVGQFEDTMTIGLQTLISYGGMDGFITLLDSSGNFLWAKQLGSVGND
                     RFSDVAFVSNSEIVASAWINQTAFLNNTLINVFNKSGLVSWFDYNGNIISYKQASNIA
                     KNSFNALCTNISNEILVAGSMEDTLQLDSIKLASVGLADMIIGKIVKRETAVTEVQDN
                     EFLVQNNLFSDETRPSIIFSEQVQIKSVEIFDMNGNCVVKYFPQRKLDVGNKFTINNP
                     VMSQLSQGVYLVRITTGISDKIVLIKIIKV"
     gene            complement(8189..9355)
                     /locus_tag="PKX59_08200"
     CDS             complement(8189..9355)
                     /locus_tag="PKX59_08200"
                     /inference="COORDINATES: protein motif:HMM:NF025330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOZ90886.1"
                     /translation="MKKILIAILLSSFNGYGQQLVWTQKADIPSSTRHSPIGFSINQY
                     GYIATGFNNGTCYNDMWKYDPSTNVWSQVASLPGSGRYGASVFVINSKAYAGIGWSSS
                     NQGLSDFYEYDPIANQWSARANFIGPQIYTAIGFAVGGLGYITSGYPLNSSVFEYNPI
                     NNVWFSKSGFPGNPRQSATSFVVNDTAYMVSGYLTSSPSNELWRYNQLSDTWTMMQSL
                     PNNPRYGAVGFELSGLGVITTGGNGTYYFTDGYAYSPPINQWLNIPQFTGAIRRHSAS
                     FTINNKGYVSCGISATGSTLNDLWELSDVTALPEAASTQPKVYYNQSYSTLTVTGLAN
                     KKSTLKIFSLSGQMVYSQQCYSDMEQIPLKLNKGIYSYNIEGIGKMMNGKFEKL"
     gene            complement(9409..10131)
                     /locus_tag="PKX59_08205"
     CDS             complement(9409..10131)
                     /locus_tag="PKX59_08205"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ90887.1"
                     /translation="MTKSYKEVAEYYDELWADLEKQSMAGVNSRHRVILNKLKKAGFN
                     KNSTLLEIGCGVGTLSNFLATQVSSGSIDAVDISPASIEYAKKKYSEVKNLSFHVSDM
                     TDFKINKKFDFILFPDVLEHIPVEAHDNIFKTISGLVHANSQVAINLPDPRSIRWLAK
                     NKPELLQIIDQDIEANVLIDKMYSNGFFLFTMETYCLYFTMPDYGWFVFKVKKEYETT
                     EKIAQSTVLLNGIKLRLRNLFS"
     gene            complement(10220..>11181)
                     /locus_tag="PKX59_08210"
     CDS             complement(10220..>11181)
                     /locus_tag="PKX59_08210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOZ90888.1"
                     /translation="TFSFNPRNKIFIPLAFIRQFFFLLFYLPSASVVVGQLAAYHTFL
                     PVLMAKLFRKPSVIFLAGTDSAFFPSIQYGNFCRPLLRWFTIASIRMATHLAPKHQSL
                     IHFNYDYDASGAPVQGVKHFIKNFKTPYTAIANGFDSNKFFPLQTERRKNTFITVAVG
                     MGTGNINQLKGVDLILQIAPLFPDCAFTLVGMSTTTLDTLPPNVKALPLQNEKQLQQL
                     YSSHQFYMQLSLSEGFPNAVCEAMLCGCIPVVSNVNALPEIAAEGFVLQHKNKDMLVA
                     LIQKALTSDSEKLSKAARLHIIKDYSLEIRKERLFALLNLLKA"
BASE COUNT         3563 a         2216 c         1935 g         3467 t
ORIGIN      
        1 cagggctacc cggttctact actgcctctt caggacttat gttggtatta atattctgat
       61 tacagaaaac agccagatta tgtaatgccg tagggctgac ataccaatta ccaccacctg
      121 catatttgag cttggcaagt ttcagtgtaa aatcaggtgc tttaaatgaa gaaaacaaca
      181 gtaccatcac taccatgatt ttcaacccaa aaagactacg gaaacgcttc atttgacccc
      241 aaaaatatgt ttaatcagtg acacaatctt atttgcacct taattttata tgaataattt
      301 tgtccttcca aaaaatcaaa tctatttaat atgacattgc gaattttact cgttgcaatt
      361 tttgcaccgc tgatgcttaa tgcagcacat gatcacaacg gtttcgaccg taatgaagac
      421 cccaatctta aagtatttgt taaagacaga aaacgtttac ctgatgtaaa ccgtcaggaa
      481 agtcttgcgc aacaaaaatc gtggcaacag ttttcggaac aaaacaaagg atggtctgta
      541 gtttttaacg aagaaaccgg attaccacac aatgcttttg ggaaaccaat ttctgtggga
      601 ctgcctttag atgctcaatc tactgcttgg tcgtttatca gtacaaagct taattctttt
      661 ggtcttccgt taaacgattt atatttcagg aatacagcac aaaacaaaaa gtacttttat
      721 gtaaactaca ttcagcgtta taatggactt gaagtattat ggtcgaaagt gcagataaaa
      781 attaccaagg atggaaaagt taatcagttt atttgcgatg catataaaga cattactgta
      841 aacattaatc caacattgag tacacaagct gcttctgctg ctgcaattaa tggtctaaat
      901 ttaacattaa atggcaccac agtaaacagt gatttaaaag ttttgcctgt gcctgatgca
      961 gcaaaaaata tttatcattt ggtttatgag gtaactgtca aagcaacaga tgcagaaaat
     1021 tttccaagag aatattatac gcttgttgat gcacataatg gcgaagtgct ttatcgtcat
     1081 aataaaatca attattttgc tgccaacaca gatgtcaatg taacagcaac agtttaccca
     1141 acacagcctt atttacctac tgctgttgag ccattagcca acatgaatgt taccatttcc
     1201 ggtgtacctt ttctgactga tgcaaatggt tatttaggac ttcctaacac ttcacctgta
     1261 acggcaactt ttaaattaga aggtagttgg gcaaatgtaa aaaccggtgc tacaacacca
     1321 cagtttacag caactttaaa tccgggtgct aacaacatca cattcagtag tgcaaatatc
     1381 aaagaacttt ctgcatacaa ttctgtgaat gaaattcatg attattatgt aaccaagatg
     1441 atgggttcag gcgctgaaac agtaatggat attcaaatgc aaaccatagt agatgtttca
     1501 ggaaactgta atgcatttta taatggcgac ttaaactttt atgctgcagg tggccaatgt
     1561 aatgcaactt ctattattaa cgatgtggtt tatcacgaat atggtcatgg aataaactat
     1621 gacttgtatt cctactatgg aggtatattc agcaatggtg cacttggaga gggatatgca
     1681 gatacatggg ctaacggatt aacagaagat cctgttttgg gaataggttt ttattccggt
     1741 aatgcaacag gttttgtaag acgttatgat cagggaagaa aagtttatcc agatgatatt
     1801 atgggtgaag tgcatgccga tggtgaaatt attgcaggtg cctggtggga tgttggttta
     1861 aactttaaca gtgtacaaca gcgtatggat ttatatgtac agacttttgc agcaactatt
     1921 gattatcctg atggacaaga aggacaactc tacaccgata ttttagttga agcattaact
     1981 attgatgata atgatggaga cctgagcaat ggaacaccaa attattgcgc catttcttct
     2041 gcctttggca tacatggaat aaacatgttc gtagtaggaa aattaataac ccataatgat
     2101 gtactcagcg ctcccggtca acaaaacatt acactcaacg cttccggtcc tgcaagtttt
     2161 ggaaatggca caggtaacag tgtaaacgga tactacagtc tgaaccattc tgctacatgg
     2221 aatccaatca gctttactta taacggaacc atttttcagg gaagtattcc cggtcaacct
     2281 aacggtacaa ttgtttcata ttatcttgat gcaacagatc aatgtggaac acactttagt
     2341 ttaacaccac ctaatgtaac agattccaat cctaatcttc cttactatgt tttggttgga
     2401 tataatttag tggcaagtga ttattttgat acaggcaaca gtcaggggtg gactttaggt
     2461 atgccgggtg ataatgcaac aacaggacaa tggataattg catcacctgt aggttctatt
     2521 cttaatcccg gaggatcacc accggtatat gttcaacctc ctattgatca cacaaacact
     2581 tctaacaacc tttgcgctgt gacagggaat gcagccccta acgatggtgc aggaaccaac
     2641 gatattgatg atggaaaaac tactttgctc tcacctaact ttgatctttc ggcttactcc
     2701 aatcctgcca tttcctactg gcgttggtac accaacgatc agggagccac accgggcaca
     2761 gactattggc aggtttctgt gagcaatgat ggcggaacaa cttttgtgcc tatcgaaaac
     2821 tgtattgttt ccgatcatag ctggagacgt tttgcatttc gtgttgcaga ctatgtgcag
     2881 ccaacagcca acatgcgact gaaatttatt ggtgaagatg caaacgatgg aagtctggta
     2941 gaaggcttgc ttgatgatct tgaaatctgg agtgcagagc ctgtaggtat ttctgaaaca
     3001 ccaatgttat cgtggtatgc ctacccgaat cctgccaatg aacaacttaa tttcggatgg
     3061 aaaggaaatg gaacaacagt tgatgtgaaa ttattcaaca ccgttggaca aatagtttat
     3121 cagaatcagt tttcgggcaa caccgtttca gaatcggtta gcctgaaagg aatggcagaa
     3181 ggcatttata ccttacagat gtcaggagaa aaattattta aatcgcagaa aatttctgtg
     3241 gtacattaat cttaaaacca aaacaaaaag ccgctgtgtt gcaaaacata gcggcttttt
     3301 ttataagtgc gtaaatatta tttgttgaat gatggtcttg cctgatgcaa tgcaggagtg
     3361 ttttcagtaa tctgaactaa gctgttacct ttaaaagtat ctcttaaatg ctgtacaaaa
     3421 gagtcttttg attttttatc ctgaccaaga agtctaaaac aataatctac ttctccttct
     3481 ctaccccatg caatttcttc gtgtgcaaca ggcattccaa cttcagtctg atattgctga
     3541 accacctgct ctaacttttc ttttgcagca ccatctgttc ctgcaccaat gctgatgaat
     3601 gaaacaataa ccctgtacat gctgtcgggc aaatctacac gtgggtttgt tgctgcaggc
     3661 tcctgtttag gcgtttccgc agttgtattt ttatttgttt tacacgagcc tgccatggca
     3721 actatcagca ccaaggcaca acaattcatc ctgttaagca tatagttcat cgtattttgt
     3781 ttttgcataa ttaaaaaaat aattgaagtt aagtacttct cccgtcacat ttttacataa
     3841 ctcatctgcc agtatcgttc ttccatgacg atgtatattg cttcttaacc acatcagtag
     3901 cggcatggtg tcaccatttt gaatatgatg catcaatgcc ggaatttctt tatttgcctg
     3961 atgaaaaaac tgtgatgcat agaaactccc taaagaatat gtcgggaaat aaccaaagct
     4021 accatgactc cagtgaacat cctgcaacac cccttttgcg tcagaaggca catctatatt
     4081 cagataggat ttatactttt cattccatac ttcaggaagt tgtgcagcct gcaatgctcc
     4141 cgacaaaata tctttttcaa tctcaaaacg aattaacaca tgcagatgat aggtaagttc
     4201 atcggcattg gtacgtataa gtgatgtttt gatgcggttt attgccttgt aaaaacttct
     4261 ttcgctaaca tcatgcaact gctcaggaaa aatatttctt agtgttttga aattatgatt
     4321 ccagaaatac tgcgatttgc ctacatggtt ttcccacagg cgcgactgtg actcatgaat
     4381 acttaaactg agatactcgc ctgatggcaa tccataatca tctgcaggca atccctgttc
     4441 atacagtgca tgtccgcctt cgtgaataca gctgccaatc atttcaaaca aatcatgctc
     4501 actgatgcgt gtagttacac gaacatcgtt tgcagaaaag gaagtggtga aaggatgcgt
     4561 agagatgtcc tgcctgccac tttcaaaatc aaagtgcatc tgctttaaca catcaagccc
     4621 aaaatgccac tgtttatctt tattgtagct tttaaataaa aaattgtcat cgggctgagg
     4681 cttctgcacc aattgctgta tgtagggcac aagttttttc cttacctgat tgaaaattgt
     4741 ttccagaaag cttgtcttca atcctgtttc ataaaggttg agatgtgcat catacggatg
     4801 ttgctcataa cccaacaact ccgactcctg tcttttaaac tttatcagtt cctgcagatg
     4861 tggttcaaac aacttaaaat tattttcttg ctttgcccgt tgccaggcat taaaactctt
     4921 tgagatacat tgattgagtg caactacaaa ttctgttgta tatttttttt gctgtttaaa
     4981 aattctgtac gactccagca cattggcgca ttgcacagga ctaagtttgg aagtatcgtc
     5041 atgcagcttt tcgataatct cttcataacg tttatcagtt attaactcat gcgcataacc
     5101 tgccagggtt gacagttgct gtgcgcggta atgtgcgcct tttacaggca tatacacttc
     5161 ctgatcccac tgcaatacag cactgctgta attgatgtct gcaatcttct gaaggtgggt
     5221 tacatattgt tgatatagtt cactcattat tgttttttaa atgcataaat aatcaagaaa
     5281 atccatcccg tcataaaaca aagcccgcct ataggagtaa caggtccaag ccatatccag
     5341 gtgttgccac taatagatga caatgccaat ccataaatag agccactaaa aagtattata
     5401 cctgtcataa aaaaagttgc agcacggtta agcattttat tttcatttgc ggcaagagca
     5461 acaaacaata aggcaagtgt gtgataaaac tgatagcgca cacccgtttc aaaggccgac
     5521 aacatctgtg gcgacaacaa ctctttaagc ttatgtgcac caaaggcacc caatgccact
     5581 gcgcaaaggc ctgatagggc ggcaaaaatc aaaatcttct tattcattat tccttatttc
     5641 ctttctcgcc tacaaaaaca tcttcctcat ctttaaacaa aacattaaag gtggtgaggc
     5701 tgccataaat acgtgtcaga tattgctgaa ggttgatttt atcttcttcg gttaatttat
     5761 catgtgcgtt gatgcgctgt tccataacgc gcagtcggtc gcgaatcata attattttat
     5821 taaagaaaac atccatagga atttctttag gcttttgtga aggattgttg ggatgcaaca
     5881 caaccttacc tccaatccag cgaccatgca aaggcacggt ctcctgcacg tctgaatatt
     5941 ttttaatgat gtttgtaaac acacgttcca tgtcagcaaa actcacaggg tcgcccacgg
     6001 gtggttgcgc atctattact tccaggtttt gatatgtttt agcaacttct attttaccac
     6061 gctcggtaaa taccgtaata taggttcgtg gttttacatt gatgaccaca ccaatgccaa
     6121 attcgggatg tctgatgcgt gaacctattc ctaattcttc catataaaat atttagtaag
     6181 tattatttaa tgcctatact gtctaactct tcaagagtaa cagagggcgg catgatattg
     6241 ttttttagtt taaaagatac aagtgttgct gtttttgagg catcctcttc tgtggcaaag
     6301 ccacgattac ccattacagc agggatgtta ggttgatgta ccataatttt tccatcaact
     6361 aaaatatcgt agccatagcc gcctaatgtg ctatcgtttg cataagtctg aacagtgaca
     6421 ttgttgatta agggggctgg agtctttact tcagccggct tattagtgca agatacaatg
     6481 aatagtatcg aaaagataaa acctattatt ctcatgtctc aaaaataaat ggttttatat
     6541 aaaagaactc aaactttaat aattttgatt aacacaattt tgtcggaaat accggttgta
     6601 attctgacta aataaacacc ttgtgaaagc tgtgacatta caggattgtt aattgtgaat
     6661 ttattcccta catcaagttt tctttgagga aaatatttta ccacacaatt gccgttcata
     6721 tcaaatatct ctacagattt aatctgaact tgctccgaaa aaataattga tggtcgagtt
     6781 tcatctgaaa ataaattatt ctgaactaaa aattcattgt cctgtacttc agtaactgct
     6841 gtttctcttt tcactatttt accaattatc atatcagcta atccaacaga agctaattta
     6901 atactatcca attgcagtgt atcttccata gaacctgcaa ctaaaatttc attgctaata
     6961 ttagtacata aagcattgaa tgaatttttt gcaatattgg atgcttgttt ataagaaatt
     7021 atgttaccat tataatcaaa ccaacttacc aacccacttt tattgaatac attgattaaa
     7081 gtattattta aaaatgcagt ttgattaatc caggccgatg caactatttc actattggaa
     7141 acaaaggcaa catcagaaaa acgatcattt cctacactac ctaattgctt agcccataaa
     7201 aaattaccgg aactatccag tagtgttata aatccatcca tgcctccata actaatcaat
     7261 gtttgtagtc caattgtcat tgtgtcttca aactgtccaa caacgcaaat tttatcttgt
     7321 tggattctaa tctttacacc tatatcataa actccacttc caaaagttct gctaacccat
     7381 ttgaaattac cgctattgtc atattttgct acgaaagcat catcgcctcc actactaatt
     7441 aatgttgcaa tatcaaaatc tgcagttgat gagaaatatc cggtaatata aatatttccg
     7501 aaatcgtcta atgcaacacc tcctcctgaa tcgtagctgc tacctcctgc ccgcctagac
     7561 caaagaacat ttcctaagcc atcaattttg gcaagaaaaa tatcatgact accatttgaa
     7621 tatactgtat tcggggctgc aaataattga tcataaaaat agcctgttag gtaacagttc
     7681 tgattcctgt caacctccaa tgcataacca acactgccat ttgttccttg aaaatcattt
     7741 gcaaacaaaa tatatccggt agggttcatc cttactaaaa acatgttatt tacaaacccc
     7801 gaattcaatg tgattaaatc aaattgtgct gaataactgt aagcacccgt tacataacaa
     7861 tttccaattg aatcagcctt aatatataga ccataaccat catttccagt tccaccaaaa
     7921 catttagtcc agagaatgtt tccattcgga ctatacttaa ctacataaat atcatcaaga
     7981 ccataactat acaatttatt tgctccacta aaaattgaat ctctgaaaat tccggcaaca
     8041 aaaacattac ctgcatagtc tgttgagaca gaaatagcag catcattatt tagcgatcca
     8101 tctgatttag cccattgcca catttgcccc tctgcattaa ctgtactcag taaaataaat
     8161 aacattgcgg aaattatatt cttaattctc ataacttttc aaatttccca ttcatcattt
     8221 taccaattcc ttcaatgttg taagaataaa ttcctttatt gagttttagc ggtatttgct
     8281 ccatatctga atagcattgt tgcgaataaa ccatctgccc cgacagagaa aatattttta
     8341 atgttgattt cttattcgcc aatcccgtaa ccgttagtgt tgagtatgat tgattataat
     8401 aaactttagg ttgagtgctt gccgcttcgg gcaatgccgt tacatcagat aattcccaga
     8461 ggtcgttcaa agtacttcca gttgcagaaa tcccacatga aacatatcct ttattattaa
     8521 tagtaaatga agcactatgt cttcttattg ctccagtaaa ctgcggaata tttaaccatt
     8581 gatttattgg aggcgagtat gcatatccat cggtaaaata ataagtaccg tttcctccag
     8641 ttgtgataac acctaaacct gacaactcaa aaccaacagc accgtatctt ggattatttg
     8701 gtaatgactg catcattgtc caagtatctg aaagttgatt atatcgccat aattcatttg
     8761 aaggacttga agtcagataa ccagacacca tataagcagt atcattgact acaaaggaag
     8821 ttgcactttg acgtgggtta cccggaaacc cggattttga aaaccaaaca ttatttattg
     8881 gattatattc aaatacagaa ctattcagag gatatccaga ggtaatataa cctaagcctc
     8941 caacagcaaa accaatagct gtataaattt gtggtccaat aaaatttgcg cgcgctgacc
     9001 actgattagc aatgggatca tattcataaa aatcactcaa cccctgatta gaagaactcc
     9061 atcctattcc tgcataagct ttactattaa tgacaaatac actagctcca tatcgtccac
     9121 ttccaggaag tgaagcaacc tgcgaccata catttgtgct tggatcatat ttccacatat
     9181 cgttatagca agttccatta ttaaaaccag ttgctatata accatactga ttaattgaaa
     9241 atcctattgg tgaatgtcta gtactacttg gaatatctgc tttctgagtc cagactaact
     9301 gttggccata accattaaat gaagataata aaattgcaat gagaatcttt ttcatataat
     9361 aattgcttca aagcaaattt ataattaaag actattaaaa acaataaatc aagaaaataa
     9421 attccgcaaa cgaagtttta tcccattaag taatacggta gattgtgcta ttttttcagt
     9481 tgtttcatat tccttcttaa ctttaaaaac aaaccaccca taatcaggca ttgtgaagta
     9541 caagcaatat gtttccattg taaaaaggaa aaatccattt gaatacattt tatcaatcaa
     9601 cacattagcc tcaatatcct gatctataat ttgtaataat tcaggtttgt tttttgccaa
     9661 ccatctaata cttcttgggt caggaagatt aatggctact tgagaattag catgaactaa
     9721 accactaatt gttttgaaaa tattatcatg tgcttctaca ggaatatgct ctaatacatc
     9781 agggaaaaga ataaaatcaa attttttatt gattttaaaa tcagtcatat cagaaacatg
     9841 aaatgaaaga ttcttcactt cactgtattt cttttttgca tattcaattg atgccgggct
     9901 aatatcaact gcatcaatac tacctgaact tacttgagta gcgagaaaat tagagagtgt
     9961 tccaacacca caaccaattt ctaataaggt tgaattttta ttgaatccgg ctttcttcaa
    10021 cttgttcaaa atgactctgt gtctgctatt gacaccagcc attgactgct tttcaaggtc
    10081 tgcccataat tcgtcataat actcagcaac ttccttataa ctcttagtca ttaaaattca
    10141 atttaaaaat aaattatgcc tgcgaaaata gtctaaaaat tcaaaccttt ccctttgtgc
    10201 taaaataaag cagttaggtc tatgctttaa gtaaattcag caatgcaaac agcctttctt
    10261 tccttatttc aagagaatag tctttgatga tatgtagcct tgctgctttc gatagttttt
    10321 ctgaatcaga agtcagtgct ttttgaatga gtgcaaccag catgtcttta tttttatgct
    10381 gtagaacaaa gccttcagca gcaatttcgg gcagtgcatt aacattagaa actacaggaa
    10441 tacaaccaca cagcatggct tcgcaaacag cattaggaaa accttcgctt aacgaaagct
    10501 gcatataaaa ctgatgcgat gaataaagct gctgcagttg tttctcgttt tgcaagggca
    10561 atgcttttac attcggtggc aatgtatcca aagtcgttgt cgacatgcca acgagtgtaa
    10621 atgcacagtc agggaaaagt ggtgctatct gcaaaataag gtcaacacct tttagctgat
    10681 taatgttgcc cgtacccata cctacggcaa cggtaatgaa tgtgtttttt ctgcgttctg
    10741 tctgtaaagg gaaaaattta ttactgtcaa aaccattggc aatagcagtg tatggcgttt
    10801 taaaattttt tataaaatgt tttacgccct gcacaggagc accggaggca tcatagtcgt
    10861 aattgaaatg aatgagcgac tgatgtttgg gggctaaatg cgtggccata cgaatacttg
    10921 caatagtaaa ccatcgcaac agcggcctgc aaaaatttcc atactgtatt gaaggaaaaa
    10981 aggcactgtc tgtccccgct aaaaaaatga ctgatggctt gcggaaaagc tttgccatca
    11041 acacaggtaa aaaagtgtgg taggcagcca gttgaccaac aactacagat gcagaaggca
    11101 gataaaataa cagaaaaaaa aactgcctga tgaatgcaag aggaataaaa attttatttc
    11161 gtggattaaa agaaaaagta a
//
LOCUS       DAOOOI010000065        10989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1166_length_10989_cov_69.991952, whole genome shotgun
            sequence.
ACCESSION   DAOOOI010000065 DAOOOI010000000
VERSION     DAOOOI010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10989
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1166_length_10989_cov_69.991952"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKX59_08215"
     CDS             complement(<1..226)
                     /locus_tag="PKX59_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90889.1"
                     /translation="MKLKFIFVFLASVLLVTDESKAQNRNSIWCFGDSAGINFSNINN
                     PVPFTSSVEGRGSCVSIADNNGNLLFYSNTW"
     gene            complement(280..7797)
                     /locus_tag="PKX59_08220"
     CDS             complement(280..7797)
                     /locus_tag="PKX59_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90890.1"
                     /translation="MKRIYSLLIYIVFINTFPALATTYTWTGNTSTAWNTTSNWSPSG
                     TPTTGDFVHIYSATYNPQLTGNLTLNGIYIHSGTLNLNGDTLLLNSGTSTFSGGTISN
                     GLLQLRGTEITFNSTTFNAPIDAVVDRINLNGCTFNNYCYFETISNVPGTGSGGAVFN
                     DSVYLYHNYTGSGIWVMNNNTGHRFNGKVTIHNKGTKEITFTGAGINVFNGDIVLINT
                     GAGGIMLGNDTLTAGHTISIGSGGFSAGDFNATNFYQDGNTAISLTFTGTALPSITGA
                     TFGGNFSITSNVLLFKNCTFSGTASFTATGSGSSSWYGGNTFYGNATFTNNATAGNLR
                     IDADTANTYYADATFNGSRELRVCYGGEVSSFYGNITTANNTTFNAGTGTLCLAGSGT
                     QALSFHSNTLVKNLMLNKSGGSAALSTALVVSGTVTFTKGTLTTTSSNLLTINNGGTV
                     SGASDSSFIDGPVKKIGNSAFVFPTGKGNSYRAVEISAPSNTTDAYKAEFFYDSISVN
                     TSNIDTTLGYVTRNRYWKLNRTNGSSNVYVSLYWGGSYAVHDTAKTYISGWNGTKWLN
                     LGKVIYGNNNSGKVTSSGTVTSYSEFALAISSTNPMLCASTPQIIFPKEYFTFDVVSG
                     SGYDYNQSSDPLTPSIASTSSSFALPSITSANIGTLSGTTQNYLKNESSAASSLTCSN
                     PVDLTKGVICVEMLVKLDANFVNQRGLRFFNFKDNSGDNAFYGGIGYPYLYLNAKVID
                     NISDPNNPIQIEKRLQIPLDGVDRKSIDYYNDQGWHHFVFKINTHNNENPPSTAPGRL
                     EIWVDGELNDFYLDDADLNNSLSILQPEFRLLDAGIDCFLGKMDNIAIYDDDVCPEQL
                     YNNYKTCFPLLTNLEPVANYCYDNTNCDLSLITSIPTTPLTGAMNPNDFPENYTINTA
                     PLSDGDLIDYINSTCSCNSSDPNWFVELDDFLQNSYDAQFTGVALPLDQLKSYPLPRY
                     FPGHSFRQNVNFMGVNYLGYSGYPRSGGSYSISANAPTYSTDIQKELSENWNYSFVVC
                     ENSSTYPNTFTQAWINAANSYPADSPVKFAITTNRNHLPNSKVKKQDFVPDEDYYLMS
                     YDWNPTPPTLSPNQFLDSDGNIVSSPKILKPLNDPTYLSNFLPDGFDQNVIFSSIFGS
                     LTKNVSYVIENNEYTPQFSYNSSGIPAVANTINGDYTLSQGCSTCATPDDWKSYLGEF
                     KKTLDNDYRDQFFGGTNFPQLADAVYLNYAVDGFDKYNKNYRFEYTNMRETQSQVMYG
                     GSSQYLSTPDFYPRRAANWLTTVSADHGIDWVNKCRTNEIDNFSDYRYAPFVAAGWEQ
                     QEEFNIRPAQWLSLVKAAGIMGVDFYNTGYFNLNAFWFANPSGYVWQAVIPSYAQAVF
                     SHAEDYFTNGTLSKGNVLLEVTDASFGYGYRFAGSLPNDLIMIRKINNEDKILITGSL
                     QPLSNISGNVPDDRDIVINDLPFSSTTSAKINFRRQGSTYIFDNTNPGDPVFYQLDKW
                     HEYKHPERWSKDFDFEAEVFDNKNVNVVIKTVDATSNSSITNGDFTNYTSYITFAATP
                     DNTGVQYNFTPRADGVNNDYDIWVRARSRSGTRTGFTITTTSVGNSNFTQDNKVSCVS
                     SGSWEWYKLDDCTGSAMTLSSLTDDQQYVLTLLPMSDDLEIDRILLDVDNNAGFTAAS
                     ACGYDKDIPDNTSSTTVSISNNDFVNIQGTYIVNSNLSLTGCIINVEAGGEIDVQSGG
                     TFSCDNCHISACGDMWNGINNTGGTVTLTSSTVNDALAAVKSVSGNAVDVTGCVFDHN
                     YTAIWLENGSYSASDIYGNTFENTGGQINKAPHAGDDPLHHIYLNNVSAVTVGDASQA
                     VNTFHNALNGIYAEASNLNVYNNIFNNIGGNNYQNRAIVPPVPPISIPNAAINARGVK
                     AGYKTLNVGGASTGQSNTFNQCEIGVSGYMQLNADIRNNAFAECYTGIALSSIGGSGN
                     TVNIEDNTIDKYNKGISLSNVWMNNSTVIKDNDLNLGAGYDAAHYGAMGIEVSNFITR
                     QVKLTIENNHIENAQNGIQVSNVGYTISPVGYAPYADVKDNTVLFAIPVGLTANHVGL
                     WLENMAYGTVENNTVHWTETPSGAGSELMQGLRLSNSVHCGITGNAIENMESGIMVFG
                     NCSQTNLYCNKFESCFNSTYFSNNNSSNQLTDQGLKVETMGTYDPTLSEGWKNHWVSP
                     VSDKAAGVLLAPNIFNWMYKPSVSFDYDPGNFTGYFTTLSADNETGSCTPAFTTDEGL
                     RDQAFGSLVYDSVIAHTDSSEIKYLMEEVFYKLMRSDTSWLHLHVPSDSVYIDRYNAG
                     KSGNAGNFATVQDSIASQNYSSASGILTGITSSLEQDLDKKFVLDCYLKYIADTLTLD
                     SATFENLNKVAVQHPLYGGEGVYFARAMLNLDIIDILPGEEESRMANTASPPENKFID
                     QQDGKLYPSPTKGRVIFEMSSAFGASAKLDIYNSYGSLAASYPLNPNARSAEFNLSGL
                     KPGVYSCRLYDSINLKMINKLVIIR"
     gene            complement(7852..8262)
                     /locus_tag="PKX59_08225"
     CDS             complement(7852..8262)
                     /locus_tag="PKX59_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90891.1"
                     /translation="MQPTKLKSGTVCGNLLAALLLFSTSSIAQPSDAYVSMKATAVAG
                     TVHGVYYIELADTNSVNQIEIKLGTDWTSADMVNAVFTYDTPGSLPQGYSYTRNGNII
                     AIDMGNFPEQSSYYGNVRVKNGATWSNNYTFVSN"
     gene            8415..9617
                     /locus_tag="PKX59_08230"
     CDS             8415..9617
                     /locus_tag="PKX59_08230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOZ90892.1"
                     /translation="MKILQLCLRIPLPPYDGATIAMYNLAESLTASGAEVTMLSFNTK
                     KHFTDINSIDAALLEKYKLETVFLDASVNAWDAFANLFKPNESYNIVRFDSEAFHQKL
                     KELLQSETFDFILFEGLFLSPYLQTVRTFSKAKCILRAHNVEWLIWKRLAKATKNPLK
                     KTYLYFLSDRLKHYENIAINKFDAIIAISQPDKNLFLKDGCNIPIEVAPTGMNTAKYK
                     NIEQMDADSLSLFHLGSMDWLPNIEAVDWLLKDIWPLINQQSKKIKLHLAGKNMSPKY
                     FNLKSENLIIEGEIKDSKKFMQDKQIMVVPLLSGGGIRIKILEGLAAGKVIVTTSIGA
                     EGINYTDKKNILIADTPQQFADTILALLNNPEKLNSIAKEAQLLARSQYNNNIIGEQL
                     VRFYQTIR"
     gene            complement(9635..10321)
                     /locus_tag="PKX59_08235"
     CDS             complement(9635..10321)
                     /locus_tag="PKX59_08235"
                     /inference="COORDINATES: protein motif:HMM:NF021788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOZ90893.1"
                     /translation="MTSPEVFQFGIPVTSVIDSINITGQEIAFYRITNIDALYSKLSS
                     LKNDDDNVIDERIPYWAELWPSAIALAEYMCTQKLNNDILIHEIGCGLALPSLVCGKM
                     GYSVMMSDYVDAPLQFASANWRLNVKSEPTTILLDWRKIPDDIEKCDLLLAADVAYES
                     RMFPAIVNAFKRLVKSGGRIVFTEPNRKFASGFFELIRHEFTNTTIQNREVIQNNHKH
                     QIKIVEIEVE"
     gene            10368..10949
                     /locus_tag="PKX59_08240"
     CDS             10368..10949
                     /locus_tag="PKX59_08240"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOZ90894.1"
                     /translation="MQIVFSFILNEINSNYQLYVVQANYPSEKESRKTFQKELNTKLL
                     KQVTKNPSLQLHYQETGKPYIDGNNAISFSHSGNYTALMITQSKSAGVDIEMTDRNIE
                     AGIDYFLNTNEIAFLSLKEKNLQALTCWCIKEAAIKYFNVSGIDFKNCIHIQPFELLN
                     LGMATVLIQHNQEEIIKICYRREKDFLLAYTVD"
BASE COUNT         3324 a         2187 c         2110 g         3368 t
ORIGIN      
        1 tccatgtatt actataaaat aaaagattac cgttgttgtc tgctatgcta acacaagaac
       61 ctctgccttc aacagatgaa gtaaaaggca cagggttatt tatgttacta aaattaatcc
      121 ctgcgctgtc gccaaagcac cagatgctgt ttcggttctg cgcttttgat tcatcggtta
      181 ctaataacac agaagctaaa aacacaaaaa tgaacttgag tttcatatag caaatataat
      241 caaaaagggg ctgctacagc aacccctttt tgaaaaatac taacgaataa taactaattt
      301 attaatcatt ttaagattaa tgctgtcgta taacctgcac gagtaaacac ccggctttaa
      361 tccggataaa ttaaattcgg cagaacgggc attgggatta agcggataag atgctgcaag
      421 gctgccgtaa ctgttgtaaa tatccagttt agcacttgct ccaaatgcgg aactcatttc
      481 aaaaataacc ctgcctttgg tgggacttgg atacagtttc ccatcctgct gatctatgaa
      541 tttattctct ggcggtgatg cggtgttagc catgcggctt tcttcttcgc caggcagtat
      601 gtctattata tctaaattaa gcattgcccg tgcaaaataa acgccctcac caccataaag
      661 cggatgctga acagctactt tgttcaggtt ttcaaacgta gcggaatcaa gcgtaagcgt
      721 gtcggcaatg tattttaaat agcagtcaag cacaaacttt ttgtcaagat cctgttctaa
      781 cgaagaggtg attccggtaa ggattccgga agccgaagaa taattttgtg aagctatgga
      841 atcctgaacc gttgcaaagt ttccggcatt gccggatttg cctgcattgt atcggtcaat
      901 ataaaccgaa tcggaaggca cgtgcaggtg aagccatgag gtatcgctcc gcatcagttt
      961 ataaaatacc tcttccatga ggtattttat ttcggacgaa tcggtatggg caataacaga
     1021 atcataaaca agagaaccaa atgcctggtc gcgcaagccc tcatcggtgg taaatgctgg
     1081 cgtgcagctt ccggtttcat tatcggcaga taatgtggta aaatagcctg tgaaattacc
     1141 cgggtcataa tcaaaagaga ctgaaggttt atacatccag ttaaatatat taggagctaa
     1201 taaaactcct gccgctttat cgctaacggg gcttacccag tggtttttcc agccttcgct
     1261 cagggttggg tcataagttc ccatagtttc cactttcaga ccctgatcag ttagctgatt
     1321 ggaagaattg ttgttactga aatacgtgga gttaaaacat gattcaaact tgttgcaata
     1381 caaattggtt tggctgcaat taccaaacac cataatgccc gactccatgt tttcaatggc
     1441 attgcctgta atgccgcaat gcacgctgtt gctcaggcgc aagccctgca taagctcact
     1501 gcccgcaccg gagggtgttt cagtccagtg cacagtattg ttttcaaccg taccgtaagc
     1561 catgttttca agccataagc ctacatggtt tgccgttaat ccgacaggta tggcaaacag
     1621 caccgtgtta tcttttacat cagcataagg cgcataaccc accgggctta tggtgtaacc
     1681 cacattgctc acctgtatgc cgttttgcgc attttctatg tggttgtttt ctattgtgag
     1741 ctttacctgc cgcgtaataa aattacttac ttcaatgccc atagctccgt aatgcgcggc
     1801 atcatagcct gcacccaggt tcaaatcatt atcttttatt acagtggagt tattcatcca
     1861 cacatttgac aaggaaatgc ctttgttgta cttgtctatt gtattgtctt ctatatttac
     1921 ggtgttgccg cttccgccaa tggagcttaa tgcaatgccg gtataacatt cggcaaatgc
     1981 attgttcctg atgtcggcat tcaactgcat ataaccggaa acacctattt cgcattggtt
     2041 aaaagtattg ctctgcccgg ttgatgcacc tcccacgttt aaggttttgt aaccggcttt
     2101 aacacctctt gcattaatgg ctgcattagg aattgaaatg gggggaacag gaggaacaat
     2161 agccctgttt tggtaattat taccgccaat gttgttaaag atattattat acacattcag
     2221 attggaagcc tcggcatata tgccgttaag ggcattatga aaggtattaa cagcctgtga
     2281 agcatcgcca accgtaacgg cagatacgtt attcaaataa atatgatgca gaggatcatc
     2341 gcctgcatga ggcgctttat taatttgccc gccagtgttt tcaaaagtgt ttccgtaaat
     2401 atcgcttgcg ctgtaactgc cgttttcaag ccatatagcc gtgtaattat ggtcaaacac
     2461 gcagcctgta acatccacag cattgcccga aacggattta acggcagcaa gggcatcatt
     2521 aacggttgag ctggtaagcg ttacggtacc tcctgtgttg ttaatgccat tccacatatc
     2581 gccacaagct gaaatatggc agttgtcaca actgaatgtg ccgcctgact gcacatcaat
     2641 ttcgccacct gcttcaacgt ttatgataca tccggtgaga gaaaggttgc tattaacaat
     2701 gtatgtgcct tgtatattca caaaatcgtt atttgaaatg gaaactgtag tgctgcttgt
     2761 gttatcagga atatccttat cataaccaca tgccgaggcg gcagtaaatc ctgcattgtt
     2821 gtcaacatcc aataaaattc tgtctatttc caaatcatcg ctcatgggca gcagcgtgag
     2881 cacatattgc tggtcatcgg taaggctgct taatgtcata gccgagcctg tacaatcatc
     2941 cagcttatac cactcccatg aaccggaact tacgcacgat actttattat cttgtgtgaa
     3001 attgctgttg cctactgatg tggttgtaat tgtaaagcct gttcttgtcc ctgatctgct
     3061 gcgtgcccgc acccatatat cataatcatt gtttaccccg tcagcccttg gtgtaaagtt
     3121 atattgcacg cctgtattgt caggtgttgc ggcaaaggtt atgtaactgg tgtaattggt
     3181 aaagtcgcca ttggtaatag aagaattaga agtcgcatca acggttttaa taaccacatt
     3241 gacattttta ttatcaaaca cttccgcttc aaaatcaaag tccttgctcc atcgttcagg
     3301 atgtttatac tcatgccatt tgtcaagttg ataaaataca ggatcaccag gattggtatt
     3361 gtcaaaaatg taggtggagc cttgccgcct gaaattaatt ttggcggatg tggttgaaga
     3421 aaacggcaaa tcattaatca caatgtctct atcatcaggc acatttcctg aaatattgct
     3481 aagcggctga agcgatccgg taatcagtat tttgtcctca ttatttattt tacgaatcat
     3541 aataagatcg ttcggaaggc ttcctgcaaa ccggtaccca tatccaaatg aagcatcagt
     3601 aacttctaaa agtacatttc ctttagataa tgttccgtta gtaaaataat cttccgcatg
     3661 gctgaataca gcctgagcgt aacttggtat tactgcctgc caaacatatc ctgacggatt
     3721 ggcaaaccaa aaagcattca gattaaaata accggtgtta taaaagtcaa cacccattat
     3781 accggctgct ttaaccaatg acagccattg tgcaggtcgt atattaaatt cttcctgctg
     3841 ctcccatccg gcagcaacaa aaggcgcata gcgatagtcg ctaaaattgt ctatctcatt
     3901 tgtcctgcat ttgtttaccc agtcaattcc gtggtcagca ctaacggtag ttaaccagtt
     3961 ggcagctctg cgcggataaa aatcaggggt agataaatat tgagaagaac cgccatacat
     4021 tacctgcgat tgcgtttctc tcatgtttgt gtattcaaat ctgtagtttt tattatactt
     4081 atcaaaacca tcaacggcat aattcaaata aacagcatca gctaattgtg gaaaattagt
     4141 tcctccaaaa aactgatcac ggtaatcgtt gtcaagagtt ttcttaaatt ctccaagata
     4201 agacttccaa tcatcaggtg ttgcacaagt actgcatccc tgagataagg tataatctcc
     4261 attaatagta ttagccactg ccggaattcc actggaatta taactgaatt gaggagtata
     4321 ttcattattt tcaataacgt aacttacgtt ttttgttaaa ctaccgaata tgctggaaaa
     4381 tataacattt tgatcaaatc catcaggtaa aaaattacta agataagttg gatcatttag
     4441 gggtttaagt atcttaggag aagaaacaat atttccatca gaatctaaga attgatttgg
     4501 agacagagta ggaggagttg gattccaatc atagctcatt aaataataat cttcatccgg
     4561 cacgaagtcc tgctttttta ccttagagtt gggtaaatga tttctattag tagtaattgc
     4621 aaattttaca gggctgtctg ccgggtaact atttgcagca tttatccatg cttgtgtaaa
     4681 agtatttgga taagttgaag aattttcaca aaccacaaag gagtaattcc agttttcaga
     4741 aagttctttt tgaatatctg tactgtaggt aggtgcgtta gcagatattg aataagaacc
     4801 tccgcttcgg gggtatccac tatatcctaa ataattaacc cccataaaat taacattttg
     4861 cctgaatgaa tgcccgggaa aataccgtgg cagaggatat gattttaatt gatctaacgg
     4921 aagagctact cctgtaaatt gcgcatcata agagttttgt aaaaaatcgt ccaattcaac
     4981 gaaccaattg ggatcagatg aattacaact acatgttgag tttatgtaat caattaaatc
     5041 accatctgat aatggagctg tgtttatagt ataattttcc ggaaaatcat tcggattcat
     5101 tgcacctgtg agaggtgtgg tgggtatgga agttattaaa ctaaggtcgc aatttgtatt
     5161 atcataacaa tagttagcaa caggctctaa attggttaga agaggaaaac acgttttata
     5221 attattataa agctgttcag ggcaaacatc atcatcataa atagcaatgt tatccatttt
     5281 acctaaaaaa cagtctattc ctgcatccaa aagccggaac tctggttgta aaatactcaa
     5341 tgaattattt aggtcagcat catccaaata aaaatcattt aattcgccat caacccaaat
     5401 ttctaatctt cccggtgcag ttgaaggtgg attttcatta ttatgagtat ttattttaaa
     5461 aacgaaatgg tgccatcctt ggtcattata ataatctatg cttttcctgt caacaccatc
     5521 taatggaatc tgtaatctct tttcaatttg aatgggatta ttaggatcgc tgatattatc
     5581 aataacttta gcatttaggt aaagatatgg atagccaata cccccataaa aagcattatc
     5641 gcctgaatta tctttaaaat taaaaaaacg taaccctctt tgatttacaa aatttgcatc
     5701 aagtttaacc agcatttcaa cacatatcac cccctttgtt aaatcaacag ggtttgagca
     5761 agttaaagaa gatgcagcag aggattcatt ttttaaatag ttttgggttg ttccagaaag
     5821 tgttccaata ttggcactgg taatactagg aagagcgaaa ctgctggatg ttgatgcgat
     5881 tgatggggtt aatggatctg aagattgatt ataatcataa ccgctaccac ttacaacatc
     5941 aaacgtaaag tattcttttg gaaaaataat ttgtggagtt gaagcacaaa gcataggatt
     6001 agtagaagag atagccaatg caaattctga atatgatgta acagtgcctg atgatgtaac
     6061 ttttcccgaa ttattatttc cataaattac tttaccaaga ttcagccatt tagttccatt
     6121 ccaccctgaa atatatgttt tcgcagtgtc atgcaccgca taactacctc cccaataaag
     6181 actgacatac acatttgatg atccattggt tctgttcagt ttccagtatc ggtttcttgt
     6241 aacatatccc aatgtagtat ctatgtttga tgtattaaca cttatggaat cataaaaaaa
     6301 ttctgcttta taagcatcag tcgtatttga aggtgcgctt atttccacag ctctgtaact
     6361 gtttcctttt cctgtcggaa acacaaatgc gctgttgcct attttcttaa caggtccgtc
     6421 aataaagctg ctgtcgcttg ctccactcac tgtgccaccg ttgtttatcg taagcaaatt
     6481 gctgcttgtg gtggtgagcg ttccttttgt aaacgttacc gttccgctta ctaccaatgc
     6541 cgttgacaat gctgcactgc caccgctctt gttcagcatc agatttttta caagcgtatt
     6601 actgtgaaag ctcaacgcct gtgttccgct gccggcaaga cacagtgtgc ctgtgcctgc
     6661 attaaaggtg gtgttgtttg ctgtggttat gtttccgtaa aaggagctta cctcaccacc
     6721 atagcacacc cttagctcgc gcgagccgtt aaaggtggca tcggcatagt aggtgttggc
     6781 agtgtcggca tcaatgcgaa ggttgcctgc cgtagcgttg ttggtaaaag tggcattgcc
     6841 ataaaatgta ttgccgccat accaactgct gctgccgcta cctgtggcgg taaatgatgc
     6901 cgtgccgcta aacgtacagt tcttaaataa cagcacattg gaagtaatgc tgaaattacc
     6961 tccaaacgtt gctcctgtta tgcttggcaa tgctgttccg gtaaaggtga ggcttatagc
     7021 agtgttgcca tcctgataaa aattagtagc attaaaatcg cctgccgaaa aacctccgct
     7081 tccaatgctg atggtatgcc ctgctgtgag cgtatcatta cctaacataa ttcctcccgc
     7141 tccggtgttt attaaaacta tatcaccatt aaatacatta atgcccgcac ctgtaaaagt
     7201 gatttctttt gttcctttgt tatgtatggt tactttaccg ttaaagcggt gtccggtgtt
     7261 gttgttcatc acccaaattc ccgaccctgt gtaattatga tacaggtaaa cactgtcgtt
     7321 aaacactgct ccaccgcttc ccgtaccggg tacattggaa atggtttcaa aataacagta
     7381 attgttaaac gtgcatccgt tcaggttaat acggtcaact actgcatcaa tgggagcatt
     7441 aaaagtggtg ctgttaaatg taatttctgt tccgcgcagt tgcaacaagc cattgcttat
     7501 ggtgccaccg ctaaaagtgc ttgtaccgga gttaagcaac aaggtatcgc cattcaaatt
     7561 aagcgttccg ctgtgtatat aaattccgtt taaggttagg ttgcctgtaa gttgcggatt
     7621 gtatgtagca ctgtaaatgt gtacaaaatc gcctgttgta ggtgttcctg atggcgacca
     7681 gttagacgta gtgttccatg cagtgcttgt gtttcctgtc catgtataag tggttgccag
     7741 tgcaggaaat gtatttataa acacaatata gatgaggagt gaatatattc ttttcatggt
     7801 atttgatttt taaatgttaa acattaaaca taatttgatt ttataataca atcagtttga
     7861 aacaaaggtg taattattgc tccatgttgc accatttttt actcttacgt ttccgtagta
     7921 agaagactgt tcgggaaaat tgcccatgtc aatggcaatg atgtttccgt tgcgagtgta
     7981 actgtaaccc tgcggcaggc tgccgggtgt atcataagta aacactgcat ttaccatgtc
     8041 ggcagatgtc cagtcggtgc cgagtttgat ttcaatttga ttcacactgt tggtgtcggc
     8101 cagttcaatg taatacactc cgtgtacagt acctgcaaca gcagtagcct tcatgcttac
     8161 gtaagcatca gatggttggg ctatgctgct tgttgaaaac aacagtagcg cagcaagcaa
     8221 gttcccgcat actgtgccgc tttttaattt ggttggttgc atgatagttg ggattttagt
     8281 ttggtagtaa tgttattttt aaagatttgt aaaattaatg agttttcctt ttcatatcca
     8341 aattaattta tcatcaaggt ttaagggtgt taattttttt aagtttgcac ataagtttag
     8401 aatccttctt taatttgaaa atccttcaac tgtgtttgcg tattccgttg ccgccttatg
     8461 atggcgccac cattgccatg tacaaccttg ccgaatcact cacggcatcg ggcgcagagg
     8521 taacaatgct ttcgttcaac accaaaaaac attttacgga cataaattcc attgatgccg
     8581 cattgcttga aaaatataaa ttggaaacag tttttcttga tgcctctgta aacgcatggg
     8641 atgcttttgc caatctgttc aaaccaaacg agtcatataa cattgtccgt tttgactcgg
     8701 aagcgtttca ccaaaagctg aaagaactgc tgcaatcgga aacatttgat ttcatattgt
     8761 ttgaaggatt atttcttagc ccttacctac aaacagtgcg tacattcagc aaagccaagt
     8821 gcatattgcg ggcacataat gtggaatggc ttatctggaa aagacttgct aaagcaacaa
     8881 aaaatccatt aaaaaaaact tatctttatt ttttatccga cagactcaaa cattatgaaa
     8941 atattgccat aaataaattt gacgcaatca ttgctatatc gcagccagat aaaaatcttt
     9001 ttctgaaaga tggctgtaat attcctattg aagtggcgcc aacaggaatg aatactgcca
     9061 aatacaagaa catagaacag atggatgctg atagtctttc attatttcat cttggatcaa
     9121 tggactggct ccctaatatt gaagccgttg actggttatt gaaagatatc tggccattga
     9181 taaaccaaca atcaaaaaag attaagctac acttagccgg aaagaatatg tcgccaaaat
     9241 atttcaatct gaaatcagaa aacctgatta ttgaaggtga aatcaaagac tcgaaaaagt
     9301 ttatgcagga taaacagatt atggttgttc cgctattgag tggtggtggc attaggatta
     9361 aaatattaga aggacttgca gcaggaaaag taattgtaac aacaagcata ggtgcagaag
     9421 gaattaatta cacagacaaa aaaaatattc ttattgctga tacgcctcaa cagtttgctg
     9481 acactatttt agccttgctg aacaatccgg aaaaattaaa cagtattgca aaggaggcac
     9541 aattgctggc aagaagccaa tacaacaaca atataattgg tgagcaattg gtcaggtttt
     9601 atcaaaccat ccgataatat ttttgcgctc tcttctactc cacttcaatt tctacaattt
     9661 taatctgatg tttgtgattg ttttgaatta cctctctgtt ttgtatggta gtgttggtaa
     9721 attcatgtct aatcaactca aaaaagccgg aagcaaattt tctatttggc tcagtgaaaa
     9781 caattctgcc tcctgattta accaatcgtt taaatgcatt tacaatggca ggaaacattc
     9841 tgctttcgta agcaacatcg gcagctaaga gcaaatcaca tttttcaata tcatctggta
     9901 ttttgcgcca atccaataaa attgttgtcg gttcggattt tacattgagt ctccagttgg
     9961 cagatgcaaa ttgcaatgga gcgtcaacat aatcactcat catcacgctg taacccattt
    10021 tgccgcaaac caatgaaggc agtgccagac cacagccaat ttcatgaatc agaatatcat
    10081 tatttagttt ctgagtacac atatactcag caagtgcaat tgctgaaggc cacaattctg
    10141 cccaataggg aattcgttca tcaatgacat tgtcatcatc attcttaagg ctgcttaact
    10201 tactatacaa tgcatctatg ttggtgattc tataaaaagc tatttcctgc cctgtaatat
    10261 tgattgaatc aattacagaa gtaaccggaa taccaaactg aaaaacttca ggcgatgtca
    10321 tgttgcaaaa ttaaaaatca acaacaaaca actgttatct tagcaccatg caaatagtgt
    10381 tttcttttat tctgaatgaa atcaacagca attatcaatt gtatgtggtg caagcaaatt
    10441 atccctcaga aaaagaaagc agaaaaacat ttcaaaaaga gcttaacaca aagttgctta
    10501 aacaagtaac aaaaaatcca tcacttcaat tacactatca ggaaacaggt aaaccataca
    10561 ttgatggtaa caatgcaatc tccttttcac actccggaaa ttatactgca ttaatgatta
    10621 cacaatcaaa aagtgccggt gttgacatag aaatgacaga tagaaatatt gaagcaggaa
    10681 ttgactattt tctaaatact aatgaaattg cgtttctttc tctaaaggaa aaaaatctgc
    10741 aagcactgac atgttggtgc attaaagaag ctgcgattaa atacttcaat gtatcaggaa
    10801 tagactttaa aaactgcata cacattcaac cttttgaatt gttaaattta ggtatggcta
    10861 ctgtgctgat acaacacaat caagaagaaa tcattaaaat ttgttacaga agagaaaaag
    10921 attttctact ggcctacaca gtagattaaa ttagtcttta gcaatttcaa catttacttt
    10981 acggccttt
//
LOCUS       DAOOOI010000066        10814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1189_length_10814_cov_16.147411, whole genome shotgun
            sequence.
ACCESSION   DAOOOI010000066 DAOOOI010000000
VERSION     DAOOOI010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10814
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1189_length_10814_cov_16.147411"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1148)
                     /locus_tag="PKX59_08245"
     CDS             complement(165..1148)
                     /locus_tag="PKX59_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HOZ90895.1"
                     /translation="MKRKTFSIALLFCIISLLVYGWGFFGHKRINRMAVFTLPAQLMR
                     FYKANIDFIEEHAVDPDKRRYAVKDEAARHYIDLDRYGESPLDSLPKNWKAAVEKYSK
                     DTLMAHGIVPWHINVMLGRLTDAFRNKDYNKILRYSADLGHYVADAHVPLHCTQNYNG
                     QLTNQVGIHGLWESRLPELFADDYDYFIGKAQVIEKPMDYIWEIISQSFAAKDSVLLF
                     EKELNARFDADRKYAFEQRGTTMMRVYSVDYSKQYQDMLNGMVERRMRQAIIAVGSFW
                     LTAWTNAGQPNLDGIEKITISEEMKREIENEDKMWRTGKVLSPGGHADDDH"
     gene            1313..2539
                     /gene="rocD"
                     /locus_tag="PKX59_08250"
     CDS             1313..2539
                     /gene="rocD"
                     /locus_tag="PKX59_08250"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOZ90896.1"
                     /translation="MSKETTVGSSYFIELEERYGAHNYHPLPVVLSRGEGIFVWDTEG
                     KQYFDFLSSYSAVNQGHCHPRIVASLVEQAGKLALTSRAFYNDKLGEYEKYATELFGY
                     DKLLPMNSGAEAVETAIKLCRKWAYKNKDIARNQAKIIFCEGNFHGRTISIISASTDP
                     TASTNYGPFTPGYVIIPYNNLAALEDALSDPNVAGFIVEPIQGEAGVVVPDDGYLSKA
                     AALCRQNNVLFIADEIQTGLARTGKMLACDYENVKPDILILGKALSGGMLPVSAVLAN
                     DNIMLAIQPGEHGSTYGGNPLACVVATTALQVILDEKLAENATKLGKILRDELNKFKH
                     EMLTLVRGKGLMNAIVIKPSKGKDAWDVCLKLAANGILAKPTHGDIIRFTPPLVMTEE
                     QLYECIAIIKKTLSEF"
     gene            complement(2536..3045)
                     /locus_tag="PKX59_08255"
     CDS             complement(2536..3045)
                     /locus_tag="PKX59_08255"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOZ90897.1"
                     /translation="MKSFIKYGIMGSLLAFAWTLVGYILGNETQEKLKWLSMLVMIII
                     VVFCFRAVINEEKKANEGFISFGKAFGMAFKTGLVMALCSAVFTYLYFNFINPEYMEF
                     AIQKAQEQMQSQGLSDEQIDMSMKMQAKFMTPAMMTIVAFFSSVIIYVIVSLIMGAIM
                     KKENPNEMS"
     gene            3174..3557
                     /locus_tag="PKX59_08260"
     CDS             3174..3557
                     /locus_tag="PKX59_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90898.1"
                     /translation="MITKIEMNHVELLEWLKALDFYKNELRFWRKHLSHVVVSNTNKD
                     ILREAEHFQNQVIIQVDQIDLLHHDVKQFENKLDEEFESVSKGGKFSQQIVDVEASLR
                     ERMKTFEHIYVDLKHDYYSFLRKYM"
     gene            3673..5988
                     /locus_tag="PKX59_08265"
     CDS             3673..5988
                     /locus_tag="PKX59_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOZ90899.1"
                     /translation="MNNYLDELNPSQRDAVECMKGPSMIIAGAGSGKTRVLTYRIIHM
                     INNQVDPFNILSLTFTNKAAREMKKRIMEKVGHDAQNLWMGTFHSVFARILRIEAEKI
                     GFPTNFTIYDSDDSKSLIKKIINEQNLDDKTYKPSLVLNRISSAKNNLFSVSDYLNHS
                     ELTNEDKMAGKPKIGMLYEIYQKRLKQSDAMDFDDLLFNTYLLLKNHPEALSKYQNKF
                     KYVMVDEFQDTNYAQYIIVRKLAAAHENICVVGDDAQSIYAFRGANIQNILNFEKDYP
                     DLKTFKLEQNYRSTKTIVGAANSVIANNRNQLKKEVWTENTGGEKIRLLRALSDNEEG
                     AQVAQAIFEDKMNLHFKNKEIAILYRTNAQSRSLEEALRKLNIPYRIFGGLSFYKRKE
                     IKDLLAYFRLTINPYDEEALDRIYNYPTRGIGKTTYEKLLVLADARTCTLWDVMQDIN
                     DREIDIATGTKTKINEFVTMMQSFSVLAKTQNAYDVASHIAQSSGLLKELFNDKTAEG
                     VSQYEHIQELLAGIKEFSDGAQTALEAPSAELEQSETKLLNEYMQDIALLTDAETDDG
                     NDDKVSLMTIHQAKGLEFKEVFVSGLEENLFPSQLAVNSRDELEEERRLFYVAITRAE
                     KRLTLSYAMTRYRWGTLTGCEPSRFLEEINPEFLDALPVSDTKMSPTAIRQGNGVPSF
                     SPNSFTKNNLKPVFKKPVAKQTDPKLFENFIPDDPKRIEIGMSVLHQRFGKGKVMQLE
                     GNLPDLKATVNFEGEGQKQLLLKYARLKIVE"
     gene            complement(5989..7419)
                     /locus_tag="PKX59_08270"
     CDS             complement(5989..7419)
                     /locus_tag="PKX59_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOZ90900.1"
                     /translation="MENIQQKNSSTSYNVVTNEILEKLKAICGEKYVVNDDESCATYG
                     SDETEDLFFKPEVIVKPSDANEISAILKLANSHFIPVTPRGAGTGLSGGALPVHGGII
                     LSTERLNKIIDIDEKNLQCTVETGVINQHLRDAVEAKGLFYPPDPASRGSCFIGGNLA
                     ECSGGPKAVKYGVTKDYVLNCEVVLPTGEIIWTGANVLKNSTGYNLTQLMIGSEGTLG
                     VITKVVLKLIPLPTETLLMLIPFRSAEKACEAVSAVFRAGITPSAMEFMERDAIEFVM
                     KFTDVKVPLKEDHVAHLLIEVDGNNKDVLFKDCEKISEVMYQHDCDEVMFAESAQQKA
                     DLWRARRAVGEAVKSHSVYKEEDTVVPRASLPVLLAGVKKIGSKYGFRSVCYGHAGDG
                     NLHVNIIKDGMTDEEWNRKLPLGIQEIFELCVSLNGTISGEHGIGWVQKNYMSIRFSD
                     VEIALQKGIKKLFDPNNILNPSKIFA"
     gene            complement(7419..>8415)
                     /locus_tag="PKX59_08275"
     CDS             complement(7419..>8415)
                     /locus_tag="PKX59_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOZ90901.1"
                     /translation="TVFVFVLLILSYTFKGGVKTIVWTDSLQTTFMLLSLFISVYLIA
                     KELNFSFADVVSSIKSSPYSDIFVWDWRSKVFFPKHFFGGMFIAIAMTGLDQEMMQKN
                     ISCRNVRESQKNMMTFSIILVFVNFIFLCLGALLYIFIQHQGIVIPERTTDDLFPTIA
                     MHYLGTFSAICFIIGLISAAYPSADGALTALTASVCIDFLGFEEGKYTEAQKTKIRKS
                     VHVIFGFVLLGVIVLFHAINDQAVIRQIFRAANYTYGPLLGLFAFGILTKRNVIDKSV
                     PVIGILSPVICYIIDVNSVQWLGGYKFGNELIILNGGITFMGLLLLTKHKTAAQE"
     assembly_gap    8416..8425
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8426..8891)
                     /locus_tag="PKX59_08280"
     CDS             complement(<8426..8891)
                     /locus_tag="PKX59_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOZ90902.1"
                     /translation="MSPVLILSVVLVYTILLFAITFYTSRNADNQSYFVGNRSSRWYV
                     VAYGMIGASLSGVTFMSVPGDVGTGNFSYFQMVLGYLLGYAVIAFVLLPVYYRMNLTS
                     IYTYLKSRLGEKTYKTGAFFFILSRVVGASFRMYIVVYVLQLFIFQAWGVPFW"
     gene            9003..9624
                     /gene="recR"
                     /locus_tag="PKX59_08285"
                     /pseudo
     CDS             9003..9624
                     /gene="recR"
                     /locus_tag="PKX59_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679580.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
     assembly_gap    9727..9812
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9813..10712)
                     /gene="hppD"
                     /locus_tag="PKX59_08290"
     CDS             complement(<9813..10712)
                     /gene="hppD"
                     /locus_tag="PKX59_08290"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HOZ90903.1"
                     /translation="MAEDFLPLNGTDHVELYVGNAKQSAYYYQMAWGYELIAYAGPET
                     GIRDRASYVLQQGKIRLVLTSAMHPDSEITRHVAQHGDGVKFLAIWVDDAEKSFYETM
                     SRGAKAHTQPTVLSDKFGEVKISAIHTYGETVHKFVERKNYNGAFLPGYSPAKSSVKT
                     TPVGLKHVDHCVGNVELGRMNEWVKFYEDVMGFKLLITFDDSDISTESALMSKVVSNG
                     NGYIKFPINEPADGKRKSQIEEYIEFYHGAGVQHVAIATDDIISTVTELRNRGVEFLH
                     VPEVYYEDVWDRVGKINEDVATIK"
BASE COUNT         3450 a         2055 c         2045 g         3168 t
ORIGIN      
        1 ttacagagcc acatttttat gtatatagca gtgctaatca atttaccgat ttaaaactgg
       61 ctgaactatg aagtccgata gttatcggta gaaacgtttg cagatgttac tgcagtttgt
      121 gcttcaagaa aaaataaaaa actattggtt gatactccaa aaaatcaatg atcatcatct
      181 gcatgtccac ccggtgacag cactttgccc gtgcgccaca ttttgtcttc gttttcaatt
      241 tcgcgtttca tctcctcgct gatggtaatt ttttcgatac catcgaggtt aggctgtccg
      301 gcattggtcc atgctgtgag ccagaagctg cctactgcaa taattgcctg tcgcattctg
      361 cgttccacca tgccgttgag catgtcctga tattgtttgg aatagtcaac tgaatagaca
      421 cgcatcatgg tggttccacg ctgctcaaag gcatatttgc ggtcggcatc gaatcgggca
      481 ttgagttctt tttcgaataa cagaacagaa tcttttgcag caaaactttg actgatgatt
      541 tcccagatgt agtccatagg tttttcgatg acctgtgctt tgccaatgaa gtagtcgtaa
      601 tcatcggcaa agagttcggg caggcgcgac tcccataagc catgaatgcc cacctgattg
      661 gtgagttgtc cgttatagtt ttgtgtgcag tgcagcggca catgtgcatc ggcaacatag
      721 tgtccaaggt cggcagagta gcgtagaatt ttgttgtagt ctttgtttcg gaatgcatct
      781 gtgaggcggc caagcatgac gttgatatgc caaggcacaa tgccatgcgc catgagggtg
      841 tctttgctgt atttttcgac agcagccttc cagtttttag gcaggctgtc gagaggagat
      901 tcgccatagc ggtcgaggtc aatgtagtga cgcgcggctt cgtcttttac ggcatagcgt
      961 cttttgtcgg gatcaactgc atgttcttca ataaaatcaa tgttggcttt gtagaaacgc
     1021 attagttgag ctggcaaagt aaagacagcc attctgttaa ttcgtttatg accaaaaaat
     1081 ccccaaccat aaaccaacag actgatgatg caaaaaagca aggcaataga aaaagttttt
     1141 cttttcatgt aaattgttga agcgttaaaa atagttgttt taatttgatt tgatatgttt
     1201 gactgaagtg atattgattg tttgaatgtt ttgacagcgt aatattctaa tgcctaccta
     1261 taaagtggcg gcaattgatt aaatttgcat cagacaattt tttattatgg aaatgagtaa
     1321 ggaaacaact gtaggatcaa gttattttat tgaactggaa gagcgttatg gtgcacataa
     1381 ctatcatccg ttgccggtag tgttgagccg tggcgaaggt atttttgttt gggatacgga
     1441 aggcaaacag tattttgatt ttttatcgtc atacagtgcc gtaaatcagg gtcattgtca
     1501 tccgcgtatt gtggcatcgt tagttgagca ggcaggaaag ttagcactta cgtcaagagc
     1561 tttttataat gacaagcttg gagaatacga aaagtatgca actgagttgt ttggctatga
     1621 taaactgttg ccgatgaatt caggcgcaga agctgttgaa acagccatta agctttgcag
     1681 aaagtgggct tataagaata aagatattgc acgcaatcag gcaaaaatta ttttttgtga
     1741 aggaaacttt cacggacgta ctatttcaat tatttctgca tctacagacc caacagcatc
     1801 aacaaactat ggcccgttta ctccgggata tgtaattatt ccatataata atctggcagc
     1861 gttggaagat gctctctccg accctaacgt tgcaggattt attgttgagc ctatacaggg
     1921 cgaagccggt gttgttgttc ctgatgatgg ctacttgtct aaagcagcag cattatgcag
     1981 gcaaaataat gttttgttta ttgctgatga aattcaaaca gggttagctc gtacaggtaa
     2041 aatgcttgcc tgcgattatg agaacgtgaa acccgatatt ttgattttgg gaaaagcatt
     2101 gagtggtggc atgttgccgg tgagtgctgt tttagctaat gataatatta tgttagcgat
     2161 tcagccggga gaacatggtt caacctatgg aggaaaccca ttggcctgtg tagttgcaac
     2221 aacagcctta caggtaatac ttgatgaaaa acttgcagag aatgcaacta aattgggtaa
     2281 aattctgcgt gatgagttaa ataaatttaa gcatgaaatg ctgacattgg ttcgcggcaa
     2341 agggctgatg aatgctattg tgataaaacc tagcaaagga aaagatgcat gggatgtttg
     2401 tttgaaatta gcagcaaatg gcattttggc caagcccact catggagata ttatcagatt
     2461 tacaccacca ttggtgatga cagaagaaca gctttatgaa tgtattgcaa ttattaagaa
     2521 aacgttgagt gaattttaac tcatctcatt aggattctct tttttcatta ttgcacccat
     2581 tatcaaagat acaattacat aaataattac cgagctgaaa aatgcaacta ttgtcatcat
     2641 tgccggtgtc ataaatttag cctgcatttt cattgacata tcaatctgct cgtcagacaa
     2701 accttgtgat tgcatttgct cctgtgcctt ttgaatggca aactccatgt attccggatt
     2761 tatgaagtta aaatataaat aagtgaaaac ggcactgcat aaagccatta ccaaaccagt
     2821 tttaaatgcc atgccaaaag ccttaccaaa agaaataaaa ccttcattag cttttttctc
     2881 ttcattgata actgccctga aacaaaacac gacaattata atcatcacca acatgctaag
     2941 ccatttgagt ttttcctgtg tttcgttacc taaaatgtaa ccaaccaaag tccatgcaaa
     3001 tgcgagcaat gagcccataa ttccatattt gataaatgat ttcatcatga attgatttaa
     3061 tgtttacaag ccaaaattag ttttttaaag catacaaaga aatttattgc cgagacagta
     3121 actaaggtaa gtcggaaatg tgctatttta gcagctacaa aagaacataa tgtatgataa
     3181 caaaaattga aatgaaccat gttgaattac tcgaatggtt gaaggcactg gatttttaca
     3241 agaatgaatt gagattttgg cgtaaacatc tgtcacatgt agttgtaagc aatacaaaca
     3301 aagatatact tcgcgaagct gaacattttc agaatcaggt tatcattcag gttgaccaga
     3361 tagacctgct gcatcatgat gtaaaacaat ttgaaaataa attagacgaa gaatttgaat
     3421 cagtaagtaa aggcggaaaa ttttcgcagc aaattgttga tgtagaagca tcacttcgcg
     3481 aacggatgaa aacatttgag catatttatg ttgatctgaa acatgattat tattcttttc
     3541 tcagaaaata tatgtaatca ttagtttgaa attaattaaa aagttttcaa tagcagggta
     3601 tgaaaaaaca taccctgttt tcattttaat aaccaaagca acccttatct ttgaagagta
     3661 gtaaattcca ttatgaataa ttatttagat gaattaaatc catcgcagcg tgatgcagtt
     3721 gagtgcatga aagggcctag tatgattatt gccggtgcag gttcgggaaa aaccagggtg
     3781 cttacctaca gaatcataca catgatcaac aatcaggttg acccatttaa tattttgtct
     3841 ttaaccttta ccaataaagc agcccgtgaa atgaaaaagc gcatcatgga aaaagttggt
     3901 catgatgctc aaaatttgtg gatgggaacc tttcactcag tatttgcacg tattttaaga
     3961 attgaagccg agaaaatagg attccctaca aatttcacca tttatgattc tgatgattca
     4021 aaaagtctga ttaaaaaaat tatcaatgaa cagaatcttg atgacaaaac ctacaagcct
     4081 tcattggtac tgaacagaat ttcgtctgca aaaaataatt tgttttctgt aagtgactat
     4141 ctgaatcact ccgagttgac caatgaagat aaaatggcag gcaaaccaaa aataggtatg
     4201 ctatacgaaa tttatcaaaa gcgattgaaa cagtctgatg ccatggattt tgatgatttg
     4261 ttattcaata cctatttgct ccttaaaaat caccctgaag cgttgtcgaa gtatcagaat
     4321 aaatttaagt atgtgatggt tgatgagttt caggacacaa actatgcaca atacattatt
     4381 gtcagaaaac ttgcagcagc acatgaaaat atttgtgttg tgggtgatga tgcacaaagt
     4441 atttatgcat tccgaggtgc taacattcaa aatattctca acttcgagaa agactatcct
     4501 gatttaaaga cttttaaatt agaacagaat tacagaagca caaagaccat agtaggcgca
     4561 gcaaacagtg taattgccaa caacagaaat caacttaaaa aagaagtctg gacagaaaat
     4621 acaggtggcg aaaaaataag attactcagg gcattaagcg acaatgagga aggtgcacag
     4681 gtagcacagg caatctttga agacaaaatg aatctgcatt ttaaaaacaa agaaattgca
     4741 attctttatc gcaccaatgc acagtctaga agtttagaag aagcattgcg taaactaaat
     4801 attccttaca gaattttcgg aggcttgtct ttttataaac gaaaagaaat aaaagattta
     4861 ctcgcttatt ttcgacttac aattaatccg tatgacgaag aagctcttga cagaatttac
     4921 aactacccta cccgaggtat cggcaaaacc acttatgaaa aactattagt tcttgctgat
     4981 gcacgtactt gtacgctgtg ggatgtgatg caggatataa atgatcgtga gattgatatt
     5041 gcaacgggaa caaaaacaaa aatcaatgag tttgttacta tgatgcaaag tttttctgtt
     5101 ttggcaaaga ctcaaaatgc ttatgatgtg gcaagtcaca ttgctcaatc aagcggattg
     5161 ttgaaagaat tatttaatga taaaactgca gaaggtgtca gtcaatacga acatatacag
     5221 gagttgctgg caggtataaa agagtttagc gatggtgcac aaacagctct tgaagcacca
     5281 tcagcagaac tggagcaatc tgaaacaaaa ttgctgaacg aatatatgca ggacattgcc
     5341 ctgcttacag atgccgaaac cgatgatggt aatgatgata aagtttcact gatgaccatt
     5401 catcaggcta agggacttga gtttaaggaa gtttttgttt ccggccttga agaaaattta
     5461 ttcccatcgc agttggctgt taactcgcgt gatgaacttg aagaagaaag gcgtttgttt
     5521 tatgttgcca ttaccagagc agaaaagcgg ctgacactaa gttatgccat gacacgttat
     5581 cgttggggaa cattaacagg ttgcgagcct agtcgttttc ttgaagaaat taatcctgag
     5641 ttcttggatg cactgcccgt tagtgataca aagatgtcac ctactgcaat aaggcaaggc
     5701 aatggtgtgc caagtttcag ccccaacagt tttacaaaaa acaatcttaa acctgtattt
     5761 aaaaagcctg ttgccaaaca aaccgacccc aaattgtttg aaaactttat tcctgatgat
     5821 cctaaaagaa ttgaaattgg aatgagtgta ttacatcaac ggtttggtaa aggaaaagtg
     5881 atgcagttgg aaggcaactt gcctgatttg aaagctaccg tgaactttga aggtgaaggg
     5941 caaaagcaat tgctgttgaa atatgcacga ctgaaaattg tagaataatc aggcaaaaat
     6001 ttttgaagga tttaaaatat tattgggatc aaagagtttt ttaattcctt tttgaagtgc
     6061 aatttcaaca tctgaaaaac gtatagacat atagtttttt tgcacccacc ctattccatg
     6121 ctctccggaa atggttccat tcaacgatac acaaagttca aatatttcct gaatacctaa
     6181 tggtaattta cgattccact cctcgtcagt catgccatct ttaataatgt ttacatgcag
     6241 atttccatca ccggcatgac catagcaaac actgcgaaat ccatatttgc ttccaatttt
     6301 ttttactcct gcaagcaata caggtaaact ggcacgaggc acaacagtat cttcctcctt
     6361 atacacagaa tgtgacttta cagcttcgcc tacagctcgt cttgcacgcc agaggtctgc
     6421 tttttgctgt gcactttctg caaacatcac ttcatcgcaa tcgtgctgat acatgacttc
     6481 tgaaattttt tcgcagtctt taaataaaac atctttgttg ttgccgtcaa cttcaatcag
     6541 taaatgcgca acatgatctt ctttaagcgg cacttttaca tcagtaaatt tcatcacaaa
     6601 ttcaatggca tcacgttcca taaactccat tgcagaaggt gtaattcctg ctctgaaaac
     6661 cgctgaaaca gcttcacatg ctttttctgc agatcgaaaa ggaatgagca tcagcaaagt
     6721 ttctgttggc aagggaatta actttaacac cacttttgtt atgacaccta atgtaccttc
     6781 actacctatc atcaactgtg tcagattata tcctgtggaa tttttcagca cattggcacc
     6841 tgtccatatt atttctcccg ttggaagcac tacttcgcaa ttcagtacat aatctttagt
     6901 aacaccatac tttacagctt taggtccacc gctacactcg gcaagattac cgccaataaa
     6961 acaacttcct ctgctggcag ggtcaggagg ataaaataag cctttggctt ctactgcatc
     7021 gcgcaaatgc tgattgatta cacctgtttc gaccgtgcat tgaaggttct tttcatcaat
     7081 gtcaataatt ttatttagtc gctctgtgga caaaataatt ccgccatgca caggcaaggc
     7141 accaccactt aaaccggtgc ctgcaccacg tggtgtgact ggaataaaat gtgaattggc
     7201 aagtttcaat attgctgaaa tctcattggc gtcagacggt tttactatta cttccggttt
     7261 aaaaaacaaa tcttcagttt cgtcactgcc ataggttgca catgattcat catcgttgac
     7321 aacatatttt tcgccacaaa tagcttttag tttttcaaga atttcattcg taacaacatt
     7381 atatgatgtg ctactatttt tctgctgtat attttccatt actcttgtgc tgcggtttta
     7441 tgtttggtta acaaaagtaa gcccataaag gtgatgccgc cattaagaat tattaattca
     7501 ttcccgaatt tatatccacc aagccactgc acagagttta catcaatgat ataacaaatt
     7561 acgggcgata atatcccaat tacaggcact gatttatcaa ttacatttct tttagttaga
     7621 attccgaatg caaaaagtcc aagcaaggga ccataggtat aatttgctgc tctgaaaatc
     7681 tgacgtatca cagcctgatc gttgatggca tgaaacaaaa cgataacacc aagtaataca
     7741 aaaccaaaaa tgacatgtac actttttctg atttttgttt tttgcgcctc tgtatattta
     7801 ccttcttcaa agccaagaaa atcaatacac accgatgctg ttagtgctgt taatgctcca
     7861 tctgcacttg gataagcagc agatatcaat ccaatgataa aacaaatagc tgaaaaagtg
     7921 ccgagataat gcatggcaat tgtcgggaac aaatcatctg ttgtgcgctc aggaataaca
     7981 atgccctgat gctgaataaa aatatacaac aatgctccaa gacagagaaa aataaaattt
     8041 acaaaaacta aaatgatgct gaatgtcatc atattttttt gcgattcgcg tacatttctg
     8101 caactgatat ttttctgcat catttcctga tcaagccccg tcatggcaat ggcaataaac
     8161 attcctccaa aaaaatgttt cggaaaaaac actttcgacc tccagtccca cacaaaaata
     8221 tctgagtatg ggcttgattt gattgatgat actacatcag caaaactaaa attaagctct
     8281 ttggcaataa gataaaccga aataaagagc gaaagcagca taaaagttgt ctgcaatgaa
     8341 tctgtccaca caattgtttt tacaccacct ttgaatgtgt atgagagtat cagcaggaca
     8401 aacacaaata cggtgnnnnn nnnnncccaa aacggaacgc cccaagcctg aaaaataaaa
     8461 agctgcaaca catataccac aatatacatt ctgaacgaag caccaactac acgtgaaaga
     8521 ataaagaaga atgcacctgt cttatacgtt ttttctccaa gcctcgactt cagataagta
     8581 tagattgaag tcagattcat acggtaataa acaggtagca acacaaaagc aatgacagca
     8641 tagcctaaca aatagccaag caccatttga aagtaagaga aatttcctgt accaacatca
     8701 ccgggtactg acataaatgt aacacctgat aacgatgccc cgatcattcc atatgcaacc
     8761 acataccaac gcgaacttct gttaccaaca aaataactct gattgtcggc attgcgcgaa
     8821 gtgtaaaatg taattgcaaa cagcagaatg gtataaacca aaaccactga taaaattaag
     8881 acaggactca attgtttgaa atttagaata caagaataac tgtttgaaaa cctttgacag
     8941 cgcatgaaag atggtatttt taaacaacgt atatttgaca acatcattta aattttcatt
     9001 aggtggagca gtatccgtca cgtttattag aagaagcagt taacgaatta tcgcgtctgc
     9061 cgggaattgg ccgtaaaggt gcattgcgcc ttgcattgca tttgctcaaa caaaatcctg
     9121 ctgatgtgga gcgtttgggc gatgctgtaa tacaattacg ttcatcggta gtttactgcc
     9181 atcaatgtca taatatttct gaaaacactt tatgtgcaat ctgtgcaaat ccaagacgtg
     9241 acggcagtgt aatttgtgtt gtacaggata ttcgtgatgt aatggcaatt gaaaacacag
     9301 cacaatttaa tggcatgtat catgttcttg gcggcattat ttctcccatg gatggcattg
     9361 gcccttcgca actgaatatt gattcgttgg ttgaaaaaaa ctgctaatgg aaatataaac
     9421 gaaataataa tggcactcag tgctaccatg gaaggtgaca caactgtgtt ttatatctca
     9481 aaaagattaa aggacattga tgttaaaatt tcaatacttg cacgaggtgt agccattggt
     9541 ggcgaattgg aatttgccga tgaagttaca ctcggccgtt cgttaattaa ccgtgtagcc
     9601 tacgaaaaca ctttggtaaa ataactgcca tcataggcag tacgtcatta caagtttcct
     9661 ctcagtgctt gctcgcgctc tatagcttca aaaagcgctt tgaagtttcc ttttccgaaa
     9721 gatttannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntttaattg tagctacatc ttcgttaatt
     9841 tttccaacac gatcccaaac atcttcatag taaacttccg gaacatgcaa gaactctact
     9901 cccctgttgc gcaactcagt taccgttgaa attatatcat cagtggctat ggcaacgtgc
     9961 tgcactccgg caccatgata aaattcaatg tattcttcaa tttgagattt gcgtttaccg
    10021 tcagcaggtt cgttaatcgg gaatttaatg taaccgtttc cattgctgac aaccttactc
    10081 attaaagcag attctgtaga aatatcgctg tcatcaaagg taatcagcaa cttaaatccc
    10141 atgacatctt cataaaactt aacccattca ttcattctcc ccaactcaac attgcccacg
    10201 caatgatcaa catgctttaa cccgacagga gtagttttta cactgctttt ggccggtgaa
    10261 tatccgggca aaaaggcccc attataattt tttcgttcta cgaacttatg tacagtttcg
    10321 ccataagtat gaatagcaga aattttcact tcaccaaatt tgtcactcaa aaccgtaggc
    10381 tgtgtgtgcg cctttgcacc acgactcata gtttcataaa aagacttttc agcatcgtca
    10441 acccaaatag ctaaaaactt aacaccatca ccatgctgcg ctacgtgtcg tgtaatctca
    10501 ctgtcaggat gcatggcact ggtcagcacc aaacggattt tgccttgttg caatacatac
    10561 gatgctctgt cacgaatacc ggtttctggg ccggcataag ctataagttc atagccccat
    10621 gccatctgat aataatatgc agattgcttg gcattgccta cataaagttc tacgtggtcg
    10681 gttccgttta acggaagaaa atcttcagcc attttgattt aatattagaa tgagaggcaa
    10741 aagtaatcat tttaaaaatg aaagtatata aacgttttat gaaagtttga cacaataatt
    10801 cctataaatt cgca
//
LOCUS       DAOOOI010000067        10686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1213_length_10686_cov_26.804534, whole genome shotgun
            sequence.
ACCESSION   DAOOOI010000067 DAOOOI010000000
VERSION     DAOOOI010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1213_length_10686_cov_26.804534"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PKX59_08295"
     CDS             complement(<1..690)
                     /locus_tag="PKX59_08295"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013751653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOZ90904.1"
                     /translation="MNKKTESLRTTEKTSHYNHLEKMSFTVLLNNINKEDHTVPRQIK
                     KNIGAIEKLAKVITDRMKKGGRLFYIGAGTSGRLGIVDASECPPTYGIAHGKVIGLIA
                     GGDAAIRKAVEFAEDDYSGGWNDLKKYKINKKDVVVGIAASGTTPYVVGALEQCRKKN
                     IATGCIVCNSGSPVAMHADYPIEIITGPEFVTGSTRMKAGTAQKLVLNMISTAVMIKL
                     GHVKGNKMVDMQ"
     gene            complement(683..1525)
                     /locus_tag="PKX59_08300"
     CDS             complement(683..1525)
                     /locus_tag="PKX59_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOZ90905.1"
                     /translation="MTEQKKKFIDVSEVIRSKNPQLYRLLPGFIINYIKRIVHETEVN
                     AFIDEHGHKTSFEFVDAIIENFGVKVEVYGQENIIKTGRCIYASNHPIGGLDAMALLQ
                     VLGHYRRDMKFIVNDILLQLNNLKDLFIGVNKHGKNSSQSIADIDALYASEMATLIFP
                     AGLVSRKQGKEIKDLQWKKSFVVKAKKYNSPIVPVYIDGRNSEWFYNLANFRKRIGIA
                     ANIEMLYLMDEMYFQRGRTISITFGKPILPQSFSGTDDGKTAEKIKEHVYELKNNRQS
                     FFHE"
     gene            1739..2206
                     /gene="mraZ"
                     /locus_tag="PKX59_08305"
     CDS             1739..2206
                     /gene="mraZ"
                     /locus_tag="PKX59_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOZ90906.1"
                     /translation="MINLFGVYESTVDAKGRVMLPSPYRKTLEKVLEEGFIIKQNVFR
                     KSLEFFPLQTWNEISQHVGKLNTFLKANQELITLFYYGVRSVELDNNGRFLIVKDHLT
                     YAGIKKDIVLSGANDRIEIWDKRAYHKYIKDNSDNLERLMEERLGGKLPENGQ"
     gene            2196..3110
                     /gene="rsmH"
                     /locus_tag="PKX59_08310"
     CDS             2196..3110
                     /gene="rsmH"
                     /locus_tag="PKX59_08310"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOZ90907.1"
                     /translation="MDSKAYHIPVMLPECIEGLKIKADGVYVDVTYGGGGHSKAIASL
                     LTTGKVIAFDRDADAIKNSLTTNKNLIVIHSSFSNITTELNSIGISSVDGVLADLGVS
                     SHQFDEPQRGFAFRFDSTLDMRMDVRQQITAATILKTYTAKELQNMFSLYGEVRNAAT
                     VAREIVAYRQHSAIATTADLRKCVSHIVPKSDENGFYARLFQALRIEVNQELKELELL
                     LQQLPDLIKAEGRLCVMSYHSLEDRLVKNFIASGNVQGAVEQDVFGNIISKTFTAINK
                     KPIEASEEEKTNNPRSRSARLRVAERIK"
     gene            3112..3501
                     /locus_tag="PKX59_08315"
     CDS             3112..3501
                     /locus_tag="PKX59_08315"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOZ90908.1"
                     /translation="MNTIRNTEEKKEKEKPNGSKIKTARKFLSMLNVIGLIDKNMLLR
                     MLPFAFFLMGLALVYIANSYVAEKTIREIDRTSRDIKELRSEYISVKSELMFKSRQSQ
                     VAKEVLPMGIKQLTVPPKKIVLKTAKD"
     gene            3508..5607
                     /locus_tag="PKX59_08320"
     CDS             3508..5607
                     /locus_tag="PKX59_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOZ90909.1"
                     /translation="MADIKKDILLRVYFVYALIFLFAMAIMGRVFQLQYVEGDYWKQR
                     SDSLTLAYKTIEPSRGNIFSADGSLLATSVPLYDLRMDMKAESITDKIFNAGIDSLAL
                     NLAALFQDKTKDQYKRMLREGRRDGERYLLVKRNVSYAELKKVKTFPIFNKGRYKGGL
                     IVEQKNMRQKPFKELASRTIGFKIGNVQPVGLEGAFDQSLRGESGKRLMQKISGNIWK
                     PLNAEDEVEAKDGCDIYTSIDLNIQDVAQQSLYNQLVKNDADAGCAILMEVATGEIKA
                     IVNLKKADSGYYREDFNYAIGYATEPGSTFKLVSMMAGIEDGYIDLEDTIDATGGRTS
                     FAPGLIMRDSHEGLNKITAKHAFEESSNVGVSKLIQKYYRKNQQAFIDRVHKMHFGDK
                     LNIQIAGEATPLIKSTTSKEWSKISLPYMSIGYETMVTPLHTLTFYNAVANNGRMVKP
                     LFVKEIRYRGHAVKEYKTQVIADSICSKSTIAKARELLEGVVQNGTGRKLKDCVYAVA
                     GKTGTAQMANNKGGYKSGRVRYQASFCGYFPADKPAYSCIVVVYNPSTAGYYGGEVAL
                     PVFKDIADKVYATNLDMHKGLDVQIAELPKVKAGLTKATIAACNNVGVDVKTVGTASK
                     WSSVDKREAVAALKPVSIKDNTVPNVEGMGLRDAIYMLETNGLQVKVVGRGAVVKQSL
                     QAGTRIYRGQVITIELS"
     gene            5607..7070
                     /locus_tag="PKX59_08325"
     CDS             5607..7070
                     /locus_tag="PKX59_08325"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOZ90910.1"
                     /translation="MQLLKEVLYKAGTEEVIGNTNVAVYGICINSRVIKPGEMYVALR
                     GTNVDGHAFILEAIEKGATSVLCEVMPDLIQAGINYIKVKNTYLALSEVCANFYHHPS
                     SSLKLVGITGTNGKTTTATLLYKLFRQLNIRCGLISTVENRVNDTVIPATHTTPDPIK
                     LNQLLSMMVLEECEYCFMEVSSHAVVQNRIGALEFAGGVFTNITHDHLDYHKTFEEYL
                     KAKKKFFDGLPESAFALTNADDKNGMVMVQNTHAAKKTYGLHGIADFRCRIIENNFSG
                     LLLQIDNTEVLCKLIGAFNAYNLLAIYATAVSLGLDKQRVLTALSMLESVNGRFDYVV
                     SGSGITGIVDYAHTPDALNNVLSTINEIRTRNEKVITVVGCGGNRDTAKRPVMAHIAC
                     EMSDKVILTSDNPRNEDPQAIIEQMRAGVPAHLSSKVMAITDRNEAIRVACNLAAAGD
                     IILIAGKGHETYQEIKGVKHPFDDKKILQENFNNLHA"
     gene            7070..8326
                     /gene="mraY"
                     /locus_tag="PKX59_08330"
     CDS             7070..8326
                     /gene="mraY"
                     /locus_tag="PKX59_08330"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOZ90911.1"
                     /translation="MLYYLFEYLDQHTNLPGMGVFKYISFRAALAIMFSLLFSFILGR
                     TIIAYLHKKQVGETIRDLGLDGQVQKQGTPTMGGLIILSAILIPTILFAKLLNVYIIL
                     MLISTVWLGAIGFLDDYIKVFKKDKKGLAGKFKVLGQIGLGLIVGSVLYFNDHVVVRE
                     KAVRSAVTWHHDNTLVDDLQSQEGVLFSTTNIKSTKTTIPFFKNNEFDYASLLGWLGD
                     GYKNLAWLIFIPVVIIIVTAVSNGANITDGIDGLAAGSSGIIGLALAVFAYVSGNTVF
                     ANYLNIMYIPNAGELLVFMSAFVGACIGFLWFNAYPAQVFMGDTGSLALGGIIAVFAI
                     AIRKELMIPVICGVFLIENLSVVMQVSYFKYTKKKFGEGRRIFKMSPLHHHYQKLGYH
                     ESKIVTRFWIVGIMLAVISLVTLKLR"
     gene            8331..9674
                     /gene="murD"
                     /locus_tag="PKX59_08335"
     CDS             8331..9674
                     /gene="murD"
                     /locus_tag="PKX59_08335"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOZ90912.1"
                     /translation="MSKNKRIVILGAAESGVGAAILAQKQGYDVFVSDLSRIADNYKT
                     HLTERNIQFEEGCHTDSLILNADEVVKSPGIPDKAAVIKKIKSANINIVSEIEFASRF
                     TNAKMIAITGTNGKSTTTLLTYHILKKAGVNVGLAGNIGKSFAWQVAEENFDHYVLEL
                     SSFQLDDMYTFKADIAVLLNITPDHLDRYEYSIQNYIDAKFRIIQNQTAEDAFIYCFD
                     DAVVKTEVLKRNPLATCYPFSIRQDEGMSAFVNDNNLYLNIHNNLFTMSIFELALQGK
                     HNLYNSMAAGVAARILDIRKESIRESFADFRNAEHRMEFVNTVHGIDFINDSKATNVN
                     SAWFALESMQKPVVWILGGVDKGNEYEMLNDLVKEKVKAIVCLGKDNKKIHEAFGSIV
                     SDITDTLSAQEAVSVAYQKGKKGDVVLLSPCCASFDLFKNYEDRGRQFKAAVKAL"
     gene            9692..>10686
                     /locus_tag="PKX59_08340"
     CDS             9692..>10686
                     /locus_tag="PKX59_08340"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOZ90913.1"
                     /translation="MNWIETYFKGDRIIWIVVILLSLMSLMAVYSSTGTLAYKYQEGN
                     TEYYLFKHGIIVFLGFGIMYFTHLVKYTFYAKVSRIAIFLAIPLLFLTLLTGTNLNEA
                     SRWLTLPVINLSFQTSDLAKIALLLYVARSLSKKQDNIKDFKSAFLPVILPVCIICGL
                     ILPANFSTAAVLFTTCLVLMFFGRINFKYILSLVGIGIAGLGLFLLVASFSGYEGRIG
                     TWKKRIENFSNGNDQGNYQTQQAKIAIASGGVFGKGPGQSTQRNFLPHPYSDFIFAII
                     IEEYGFVGGLVIVVLYLLLFYRAVVIVRKSPFAFATLLAIGCAFSLVFQAMINMAV"
BASE COUNT         3310 a         1873 c         2217 g         3286 t
ORIGIN      
        1 ctgcatgtca accattttat tgcctttaac atgaccaagt tttatcatca ctgccgtact
       61 tatcatattc agaactagct tctgtgcagt tcctgctttc atgcgagtgc tgccggttac
      121 aaattcaggc cctgtaataa tttctatagg ataatcagca tgcatggcaa caggagagcc
      181 gctattacaa acaatgcatc ctgtagcaat atttttcttg cggcattgtt caagagcacc
      241 tactacatag ggcgtagttc ccgaagctgc aatgcctact acaacatctt ttttatttat
      301 tttatatttt ttaagatcat tccatccccc tgaataatca tcttctgcaa actcaacagc
      361 cttgcgaatg gcagcatcac ctccggcaat aagtccaata acttttccat gcgcaatacc
      421 ataggttggt ggacactcag aagcatcaac aattcctaat ctgccactgg tgccggcacc
      481 aatataaaat aaacgtccac cttttttcat tctgtcagta atgacttttg ctagtttttc
      541 aattgcccca atattttttt tgatctgtcg cggaacagta tggtcttcct tattgatgtt
      601 attcagcaat actgtaaaac tcatcttttc gagatgattg taatgcgaag ttttttcagt
      661 agtccgcagg ctttctgttt tcttattcat gaaaaaaaga ttgtcggtta ttttttagct
      721 cataaacatg ctccttgatt ttctctgcag tttttccgtc atcagttcct gaaaagcttt
      781 gtgggagaat aggttttccg aatgtgatgg agatggttct gccacgctga aaatacattt
      841 catccatcag gtaaagcatt tcaatgtttg ctgcaatacc aattcgcttt ctgaagttgg
      901 ctaaattgta gaaccattct gagtttcgtc catcaatata aaccggcaca ataggactgt
      961 tgtatttttt tgctttcact acaaaacttt ttttccattg caggtctttg atttcttttc
     1021 cttgtttgcg cgataccaat cctgcaggaa aaatcaatgt tgccatttcc gaagcatata
     1081 aagcatcaat atctgcaata gactgtgagc tgtttttgcc atgcttgttg acaccgataa
     1141 ataaatcttt aaggttgttt agttgcaata aaatgtcgtt gacaataaat ttcatgtcgc
     1201 gcctgtagtg acccaatacc tgtagcaatg ccatggcatc aagtccacca ataggatgat
     1261 tggaggcgta aatacaacgg ccggttttta tgatattctc ctgaccataa acttcaactt
     1321 ttactccgaa attttcaata atggcatcta caaactcaaa cgatgtttta tgtccatgct
     1381 catcaataaa tgcatttact tcagtttcgt gaacaatgcg tttgatgtag ttaatgataa
     1441 atcccggcag cagacgatac agttgcgggt tcttgcttcg tatcacctcg ctgacatcaa
     1501 taaacttttt tttctgctca gtcataaatt ttctctctgt cgtatagcat tttttaagga
     1561 cacaaaatta agcactgctt aaattctatc gtagcctata aaatcccaaa taaaaatgtt
     1621 caggtgggag gaaaattgca gaaaggggaa agttttcaac aaatttggta aaaagtgggg
     1681 gtaggtggga aatttatttt tacattcgca caagaatttt ttaacgcacc atttaataat
     1741 gataaatctg tttggagttt acgaaagcac cgttgatgcg aaaggtcgtg tcatgctgcc
     1801 ttctccttac agaaaaacat tagagaaagt tttggaagaa ggatttatca ttaagcagaa
     1861 tgttttcaga aaatcattag agttttttcc gcttcagaca tggaatgaaa tatcgcaaca
     1921 tgtaggtaag ctgaatacat tcttaaaagc caatcaggag ttgattacac ttttttatta
     1981 cggtgtgcgc agcgtggagt tagataataa cggacgattt ttaattgtga aagatcatct
     2041 gacttatgca ggaattaaaa aagatattgt gctgagtggt gcaaacgata gaattgaaat
     2101 ttgggataag cgtgcttatc ataaatatat taaagataac agcgacaatt tagagcggtt
     2161 gatggaagag cgacttggcg gcaaattgcc tgaaaatgga cagtaaggca tatcacattc
     2221 ctgtcatgtt accggagtgt attgaagggt tgaagattaa agcagatgga gtttatgttg
     2281 atgttactta tgggggcggg ggacattcaa aggcaatagc ctctttattg acaacaggaa
     2341 aagtgattgc attcgacaga gatgccgatg caataaaaaa tagtttaacc accaataaaa
     2401 atctgattgt gattcacagc agtttttcaa atataaccac cgagctgaac agcataggca
     2461 tatcttctgt agatggcgtg cttgccgacc ttggtgtgtc ttcacatcag tttgatgaac
     2521 cgcagcgtgg ttttgctttt cgcttcgata gtacacttga tatgcgcatg gatgtacgtc
     2581 agcaaattac tgctgcaaca attcttaaaa catatactgc aaaagaattg cagaacatgt
     2641 tttctcttta tggtgaagtg cgtaatgcag ctaccgttgc tagggagata gttgcttatc
     2701 gtcagcattc agctattgca accacagccg atcttagaaa atgtgtttca cacatagtgc
     2761 ctaagagtga tgagaatggt ttttatgcaa gattgtttca ggcattacga attgaagtta
     2821 atcaggaatt gaaagagttg gaattgttgc tgcaacaatt gccggattta ataaaagcag
     2881 aaggtcgtct ttgtgtgatg agctatcatt ctcttgaaga cagactggta aaaaatttta
     2941 tcgcttcagg aaatgtgcaa ggtgcagtag agcaagatgt gtttggcaac atcatttcaa
     3001 aaacatttac tgcaataaat aaaaaaccga tagaggctag tgaagaggaa aaaaccaata
     3061 acccgcgctc aagaagtgca cgattaagag tagctgaaag aataaagtaa catgaacaca
     3121 ataagaaata cagaggaaaa aaaggaaaaa gaaaagccga acggcagtaa aataaagact
     3181 gcacgtaagt tcctttctat gcttaatgtt attgggctga ttgataaaaa tatgttgctg
     3241 cgtatgcttc catttgcttt tttcttaatg ggactggcat tggtttatat tgcaaacagc
     3301 tatgttgcag agaagaccat tcgcgaaatt gaccgcacat cacgcgatat taaagagctg
     3361 cgatctgaat atatttcagt aaagtctgag ttgatgttta aaagccgtca gtcgcaagta
     3421 gcaaaagaag tgcttcctat gggcattaag caactgacag ttccgccaaa gaaaatagta
     3481 cttaaaacag caaaagacta agagcgcatg gcagatatta agaaagatat tttactgaga
     3541 gtatattttg tttatgcctt gatttttctt tttgccatgg ctattatggg tcgtgttttt
     3601 cagttgcagt atgttgaggg tgattattgg aaacaacgct ccgacagttt aaccttagcc
     3661 tataaaacca ttgagccatc aagaggaaat atttttagtg ccgatggaag tttgttagcc
     3721 acatcagtgc ctttgtatga tttacgtatg gatatgaagg cagaaagtat tacagataaa
     3781 atatttaatg caggtattga ctcactggct ttaaaccttg ctgcattgtt tcaggataaa
     3841 acaaaagatc agtacaagag aatgcttcgc gaaggcagac gcgatggcga acgctatttg
     3901 cttgttaaac gtaatgtaag ctatgctgaa ctgaaaaaag taaagacgtt tccgattttt
     3961 aacaaaggcc gttataaagg tggtctcatt gttgaacaaa aaaatatgcg tcagaaacct
     4021 ttcaaggaac ttgcttccag aacaattggt tttaaaattg gaaatgttca acctgttggc
     4081 cttgaaggcg ccttcgacca aagtttgaga ggagaaagtg gcaagcgcct gatgcaaaaa
     4141 atatcgggta acatttggaa accgctcaat gcagaagatg aagttgaagc aaaagatggc
     4201 tgtgatattt atacatcaat agatttgaac atacaggatg ttgcacaaca atcactctac
     4261 aatcagttgg tgaagaacga tgctgatgca ggatgtgcca tactgatgga agttgctacc
     4321 ggagagataa aagcaattgt aaatctgaaa aaagctgact ccggttatta tcgagaagat
     4381 tttaattatg ctataggcta tgccacagaa cccggctcca cttttaaatt agtgagtatg
     4441 atggccggaa tagaagatgg ttatattgac cttgaagata ccatagatgc cacaggtgga
     4501 agaacaagtt ttgctcccgg actgattatg cgcgactcgc atgagggatt gaataagata
     4561 actgcaaaac atgcatttga agaatcatca aatgtgggcg tttctaaact cattcagaag
     4621 tattaccgga aaaatcaaca ggcatttatt gaccgtgtac ataaaatgca ttttggagat
     4681 aaactcaata ttcagattgc aggagaggct actccgctta ttaagtcaac tacaagtaag
     4741 gagtggagca aaatttcatt gccttatatg tctattggtt atgaaaccat ggtaacacca
     4801 ttgcacacac ttactttcta taatgctgtt gccaataatg gcagaatggt aaagcccctt
     4861 ttcgtaaaag aaattcgcta ccgtggacat gctgttaaag aatataagac acaggttatt
     4921 gcagattcta tttgctcaaa atcaacaata gcaaaagcaa gagaacttct cgaaggtgta
     4981 gttcagaatg gtaccggacg caagttgaaa gactgcgtat atgccgttgc cggtaaaaca
     5041 ggtactgcac aaatggcaaa taataaaggt ggttataaga gtggaagagt aagatatcag
     5101 gcatcgttct gtggttattt tcctgctgac aagcctgcat attcatgtat tgtagtagtg
     5161 tataaccctt caacggcagg ttactatggt ggcgaggtag cattacccgt attcaaagat
     5221 attgcagata aagtttatgc aaccaatctc gatatgcaca aaggacttga tgtgcaaata
     5281 gcagaattac caaaggtaaa agcaggtctg actaaagcaa caatcgctgc atgtaacaat
     5341 gttggtgttg atgtaaaaac tgttggcact gcatcaaaat ggtcatcagt tgataagaga
     5401 gaagctgttg cagcattgaa gcctgttagc attaaagata atactgttcc aaatgttgaa
     5461 ggaatgggtt tgcgtgatgc aatttatatg ctcgaaacaa atggattgca ggttaaagta
     5521 gttggacgtg gtgctgtggt gaagcaatca ttacaggcgg gaacaagaat ttatagaggt
     5581 caggtaataa caatagagtt aagttgatgc agttactgaa agaagtttta tacaaagctg
     5641 gaacagagga ggtcattgga aataccaatg tagctgtata tggaatatgc attaactcac
     5701 gcgtaattaa acccggagaa atgtatgttg cacttcgcgg taccaatgtt gatgggcatg
     5761 cttttatttt agaagccata gaaaaaggcg ctacttcagt tttatgcgaa gttatgccag
     5821 atctcattca ggcaggtatc aattatataa aagtaaaaaa tacctatctg gcactttctg
     5881 aagtgtgtgc aaacttttat caccatcctt cttcttctct aaaattagtt ggaattaccg
     5941 gaactaatgg taaaacaact acagccacat tgctctataa attattccgc caactgaata
     6001 ttcgttgcgg attaatttct actgtagaaa acagagttaa cgatactgtt attcctgcaa
     6061 cacatacaac acccgatcca ataaaactca atcagctatt aagcatgatg gtgttggaag
     6121 aatgtgagta ctgctttatg gaagtcagct cacatgctgt ggtacaaaac agaataggag
     6181 cattagaatt tgccggtggt gtttttacca atatcacaca cgatcatctt gactatcaca
     6241 aaacttttga agaatatctg aaagcaaaaa agaaattttt tgacggtctt cctgaaagtg
     6301 catttgcatt gactaatgcc gatgacaaaa acggaatggt gatggttcag aatacacatg
     6361 ctgccaaaaa aacttatggt ctgcatggta ttgccgattt cagatgcaga atcattgaaa
     6421 ataattttag tggtctgttg ctgcagattg ataatacaga agtgttgtgt aaacttattg
     6481 gtgcattcaa tgcctataat cttttagcaa tttatgcaac tgctgtttca ttaggattgg
     6541 ataagcagcg agtgcttact gcattgagta tgctcgaatc tgtcaatggc agattcgatt
     6601 atgttgtttc cggttccggt attactggca ttgttgatta tgcacataca cccgatgcat
     6661 tgaataatgt actttctacc atcaacgaaa taagaacaag aaatgagaaa gtaattacag
     6721 tagttggttg cggtggaaat cgtgatacag caaaacgccc tgttatggct cacattgcct
     6781 gcgagatgag tgataaggtt attcttactt ctgacaatcc gcgaaacgaa gacccccaag
     6841 caatcattga acagatgcgt gccggtgtgc ccgcacattt atcatctaaa gtgatggcaa
     6901 taaccgatcg caatgaagcc attcgtgtgg catgcaacct tgctgctgca ggagatatta
     6961 tcctcattgc aggaaaaggc catgaaacct atcaggaaat taaaggagtg aaacaccctt
     7021 ttgacgataa aaaaatatta caggaaaact ttaataacct acacgcctga tgctatacta
     7081 tctctttgaa tatttagatc aacatacaaa tttgcccgga atgggagtgt ttaaatacat
     7141 ctccttccga gcagcattgg caattatgtt ttcattgttg ttttctttta ttctcggacg
     7201 aacaataatt gcttatctgc ataaaaaaca agttggcgaa accattcgtg atcttggact
     7261 tgacggacaa gtgcaaaagc aaggcacacc tacaatgggt ggtctcatta ttctttcggc
     7321 tattcttatt ccaacaattt tgtttgctaa actgcttaat gtttacatca ttctgatgtt
     7381 gatttcaaca gtatggctgg gtgcaattgg ttttcttgac gactatatta aagtatttaa
     7441 gaaagataaa aagggtcttg caggaaagtt taaagttctc ggacaaatag gtcttggtct
     7501 catcgttggc agtgttttgt attttaatga tcatgtagtt gttcgcgaaa aagcagttcg
     7561 ttctgcagtt acctggcatc atgacaatac tttagttgat gatcttcaaa gtcaggaagg
     7621 agttttgttt tcaaccacaa atattaaatc aacaaaaaca acaattccgt ttttcaagaa
     7681 taatgagttt gattatgcct ctttacttgg atggttgggt gatggatata aaaatcttgc
     7741 atggcttatt tttattcctg ttgtaattat tattgttaca gcagtttcaa atggtgcaaa
     7801 catcactgat ggtattgacg gacttgctgc cggcagctca ggcattattg gtttggcact
     7861 tgcagttttt gcttacgtat ccggtaacac ggtttttgcc aactacctta acattatgta
     7921 cattccaaat gcaggtgagt tgcttgtttt tatgtcggca tttgttgggg catgtatagg
     7981 atttttatgg ttcaacgcct atcctgcaca ggtgtttatg ggcgacacag gaagccttgc
     8041 cttaggtggc attattgcag tgtttgctat tgctatcaga aaagaattga tgattcctgt
     8101 tatctgcggt gtttttctca ttgagaatct ttctgtagtg atgcaggtaa gttattttaa
     8161 atacacaaag aaaaaatttg gtgaaggcag acgcattttt aaaatgtcgc cactgcatca
     8221 ccactatcag aaacttggat atcatgaatc taaaattgta acccgtttct ggattgttgg
     8281 aatcatgctg gctgtaatca gtctggtaac attaaaactg cgataggtaa atgtcaaaaa
     8341 acaaacgcat agttattttg ggtgctgccg aaagcggagt aggcgcagcc atacttgcgc
     8401 agaagcaagg ctatgatgtt tttgtatcag acctatcgcg tattgccgac aattacaaaa
     8461 cacatctgac agagcggaat attcagtttg aagaaggttg ccataccgac agtctgattc
     8521 ttaatgcaga tgaggtagtt aaaagccccg gtattcctga taaggcagca gtgattaaaa
     8581 aaattaagag tgcaaacatc aatatagttt cagagattga atttgcttca aggtttacca
     8641 atgctaaaat gattgccatc acaggcacca atggcaagtc aacaacaact ttactgacct
     8701 atcatattct taaaaaggca ggtgttaatg taggtcttgc cggaaatata ggtaagagtt
     8761 ttgcctggca ggttgcagaa gaaaattttg accattatgt gcttgagtta agcagctttc
     8821 agttagatga tatgtacacc ttcaaagctg atattgctgt gttactcaac atcacgcccg
     8881 atcatcttga ccgttacgag tattcaatac aaaattatat tgacgctaaa ttcagaatca
     8941 tacagaatca aactgccgaa gatgcattta tctattgctt cgatgatgca gttgtaaaaa
     9001 cagaagttct gaaaagaaat cctttggcaa cgtgctatcc tttttcaatc aggcaggatg
     9061 aaggcatgag tgcttttgta aatgacaata acttatatct gaacatacac aacaacttat
     9121 ttactatgtc tattttcgaa ctagccttac agggcaaaca caacctctac aacagtatgg
     9181 ctgccggagt tgcagcacgc atactcgaca ttcgcaagga gtctatccgc gaaagctttg
     9241 cagattttcg caatgcagaa catcggatgg agttcgtaaa taccgtacac ggtattgact
     9301 ttatcaacga ttcaaaggca acaaacgtta acagtgcatg gtttgctctc gaaagcatgc
     9361 agaaacctgt tgtttggatt ctgggcggtg ttgataaagg taatgagtac gaaatgctga
     9421 acgatcttgt aaaagagaaa gtgaaagcta ttgtttgtct tggtaaagac aataaaaaaa
     9481 tacatgaagc attcggaagt attgtaagcg atattactga tactttgtct gcgcaggaag
     9541 cagtaagcgt tgcctatcag aaaggcaaaa aaggtgatgt agtattgttg tcaccatgct
     9601 gcgcaagttt cgatttgttt aaaaattacg aggatcgtgg tcgtcagttt aaagctgctg
     9661 taaaagcact ttaagattta attcaaaaaa aatgaattgg atagaaacat attttaaagg
     9721 cgaccgcatc atctggattg tggtaatact gctttcgtta atgtcactta tggccgttta
     9781 tagttccaca ggcactttgg catataagta tcaggaaggt aatacagagt attatttatt
     9841 taagcatggt attattgttt ttcttggttt tggtataatg tattttacac atcttgtaaa
     9901 atatactttc tatgcaaaag tttcacgcat agcaattttt cttgctattc cacttttgtt
     9961 tcttacactt cttacaggta ctaaccttaa cgaagcatca cgttggctga cactgcccgt
    10021 aatcaatctt tcgtttcaga catctgactt agctaagatt gcattgttgt tgtatgtagc
    10081 acgatcgctt tctaaaaaac aggataacat caaagacttt aagtctgctt ttcttcctgt
    10141 aattcttcct gtttgtatca tctgtggatt aattcttccg gctaactttt ctaccgcagc
    10201 agtattgttt accacctgtc ttgtgctgat gtttttcggt cgtataaatt tcaagtatat
    10261 actttctctt gtaggtatcg gtattgcagg gctgggtttg tttttattgg ttgcctcatt
    10321 ttcaggatac gaagggagaa ttggtacatg gaaaaaacgg attgaaaatt tcagcaatgg
    10381 aaatgaccag ggaaactatc agacacaaca ggcaaaaatt gcaattgcct caggtggtgt
    10441 ttttggtaaa ggacccggac agagtacgca aagaaatttt cttccacatc cttattccga
    10501 ttttattttt gcaataataa tagaggagta tggttttgtt ggtggtttgg taatagttgt
    10561 tctttatctg ttgctgtttt atcgggctgt cgtcattgtt cgaaagagtc catttgcttt
    10621 tgcaacattg cttgcaatag gctgtgcatt cagcctagta ttccaggcta tgataaatat
    10681 ggcagt
//
LOCUS       DAOOOI010000068        10495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1247_length_10495_cov_10.634962, whole genome shotgun
            sequence.
ACCESSION   DAOOOI010000068 DAOOOI010000000
VERSION     DAOOOI010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10495
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1247_length_10495_cov_10.634962"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PKX59_08345"
     CDS             <1..459
                     /locus_tag="PKX59_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOZ90914.1"
                     /translation="SMGSLAAGINSLTSTSVVDFYFRSSKKEHSDSKKLYYSKIATLL
                     WGIFCILCAFFAGNTGNLIEAVNILGSLFYGTILGIFISAIFVKHLDGNSVFIAALIT
                     EAIVIAFWYFDVMAFLWLNLAGCLLVVLIALAIQFCTQNLNRMFNKTISK"
     gene            505..822
                     /locus_tag="PKX59_08350"
     CDS             505..822
                     /locus_tag="PKX59_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOZ90915.1"
                     /translation="MQEITVHELKQWLDEGKNFQLIDIREEYEYDACNLNGTLIPMGE
                     IIDRTEEIAKDKPVVIHCRSGSRSAAIYQVLSTQYGFTNLFNLKGGIKAYSQEIDPTL
                     NVI"
     gene            832..1476
                     /locus_tag="PKX59_08355"
     CDS             832..1476
                     /locus_tag="PKX59_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOZ90916.1"
                     /translation="MEHLKFLIDFILHIDKHLFELVQRYDTWIYLILFLIVFAETGLV
                     VTPLLPGDSLLFAAGALAAPPDNPLNVFYLMIIFFFAAFGGDNTNYFIGNYIGPKVFE
                     KNYRFLKKDYLNKTQEFYEKHGGKAVIFARFVPIVRTFAPFVAGVGSMTYNRFIGFSV
                     LASILWINICVWAGYFFGRLEIVKNNFSLVVIAIIGISLVPILIGIIKQKFSSK"
     gene            1473..2195
                     /locus_tag="PKX59_08360"
     CDS             1473..2195
                     /locus_tag="PKX59_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOZ90917.1"
                     /translation="MRNFGLIGFPLSHSFSQKFFTDKFKKLNLKCVYNNYELSNLNNL
                     RELISTNQLTGLNVTSPYKTGVIPYLDRLEETAEAIGSINTILIKDKKLIGYNTDIIG
                     FKISLERLIGNQKPPTLILGTGGSAKTVAFVLKQKNIPFQFVSRKKKEGAISYSQSDD
                     FLSQNILIINCTPIGRFPDFGKKPPINYTKLTDKHFLFDLNYNPEITAFMTEGINKGC
                     KVCNGYDMLIEQAEASWVIWNT"
     gene            2312..2860
                     /locus_tag="PKX59_08365"
     CDS             2312..2860
                     /locus_tag="PKX59_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90918.1"
                     /translation="MNNILKDNKTKFITLSILVVAISRLLPHPYNFTPIGAIALFGGT
                     FLKDKKMAFGLPLAALLVSDLLLQLIKPGSAFYEGWFWVYLSFILITPLGFVLRNKLK
                     VQNVVAASLMGSIIFFVLTNFGVWATGLMYPLTGAGLMSCYVSAVPFFGGTIMGDLFF
                     NALLFGVFAFAKWRMPVLVKEA"
     gene            2895..4256
                     /locus_tag="PKX59_08370"
     CDS             2895..4256
                     /locus_tag="PKX59_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOZ90919.1"
                     /translation="MYFNNILETIGNTPLVKLNSVTQGVQATVLAKIETVNPGNSIKD
                     RMALKMIEDAEKSGKLKPGGTIIEGTSGNTGMGLAIAAIIKGYKCIFTTTDKQSKEKV
                     DALRAFGAEVIVCPTNVEPEDPRSYYSVAARLSKEIPNAFYPNQYDNLSNRQAHYEQT
                     GPEIWEQTEGKITHLVVGAGTGGTITGTGRYLKEKNPKIKVWAIDTYGSALKKYHETG
                     ILDKNEIYPYVTEGIGEDFIPQNYDFTVIDHFEKVTDKDAALMTRDLVRKEGIWVGNS
                     AGSAVAGLLQMKEQLKPTDVVVIIFHDHGSRYIAKIFNDDWMRSKGYFDKKGMTASDI
                     IGSKKDVNLTTVEATLPIGDTLKIIKTNNFSQIPITREGRIVGNITENHIYSSLLKSP
                     ELKNKPVSDIMQEAMPFIDINTSIDTLSDLLSKNNQAVIVKDFKADRNYIVTRQDVVD
                     ALA"
     gene            4276..5049
                     /locus_tag="PKX59_08375"
     CDS             4276..5049
                     /locus_tag="PKX59_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020082107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOZ90920.1"
                     /translation="MSFGICEMSLVPMRREPSHRSELVSQILFGEHYEVVESQKEWFK
                     IRTSHDGYEGFIALNQHTEISYNDFQFLQKNGAFVCFDLVQILLHDNKIQTVILGSAL
                     PFFENQHCRINTRTYRFDGNAKFPQKAATSALLIENAYMYLNAPYLWGGRSPFGIDCS
                     GFTQLVYKLSGYSLLRDAALQAEQGNIVNLVDEAMPGDLAFFDNEEGNIIHVGILTAR
                     DTIIHASGKVRIDAFDQVGIYNQETKRYTHKLRLIKRIF"
     gene            5058..5312
                     /locus_tag="PKX59_08380"
     CDS             5058..5312
                     /locus_tag="PKX59_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90921.1"
                     /translation="MNNPQLKASENFPATVNELYDEEGELVLILYTHELVDLPEGLWL
                     STEGYYEAVIKKTEIQIEDALFCISELNSISGMQYELTLS"
     gene            complement(5309..6379)
                     /locus_tag="PKX59_08385"
     CDS             complement(5309..6379)
                     /locus_tag="PKX59_08385"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ90922.1"
                     /translation="MSKTVIVAPLNWGLGHATRSKTVIDNLLAAGCKVIIASDGNSLS
                     VLKRQFPVLQAEELPSYSPVYPLNGNMVWAMLKQTPKMLLNISNEQSAIKKIASKYNA
                     EIIISDNRYGCFTNFTKNIFLSHQLMIQSPESLLFLRPSINRLHAKYINRFHECWIPD
                     VEHGFKLSGILSENKFIKIPKYNCGLLSSFQSSNLSNDKKYDIVAILSGPEPQRTIFE
                     GKIIEQLEKLHCSALLIRGKISDPNEIPDRGALQFKNYMPADEIAKFINTDTLVICRS
                     GYSTIMDIAQLGVRALLVPTPGQTEQEYLASYLEENKIFMMQTQSNIDIQNAIEKKLE
                     YAAIRIEKNILLQQNLNRILLS"
     gene            complement(6407..8695)
                     /locus_tag="PKX59_08390"
     CDS             complement(6407..8695)
                     /locus_tag="PKX59_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOZ90923.1"
                     /translation="MMQKWIKVLSIISLLNVNILFAQKVGLVLSGGGARGFAHIGVIK
                     ALEENGIPIDFITGTSAGALVGGMYVSGTSPQQMQNIVLSDDFKNWATGTLSDELIYF
                     FKERERDASWATVKLSYDSTLRMNLNGALVNSGQIDFALLENHSAASAGADYDFNNLF
                     VPFRCVASDIKNKRPVIFNCGDLSQAIRASIAYPLYLSPMTADNQLYYDGGIYNNFPS
                     DVMLNEFDPDIMIGVSVGGGPDIPKEDNMFSQLRTMIIQSQHVVLPGADDILIQPDVT
                     NIGVFDFYNFQALIDSGYKATMARMDDIKQAIARRISPEELTQKRDNFISKLHPLIVG
                     DIVVTGVSPEKTKYIKSILNPRGSNMLLRQLKPRYFKLLADENIRYAFPKLVYDPKTE
                     VYNLEIYIKPETDLRVDIGGNFSSRPISEGFIGFRYNILGPYSLSIHANTYFGKLYNS
                     GQLKLRLDIPGRPDFFIEPFTTLNRMDYFKSSSFFKDDIKPAYLLVTDQSYGLNIGMP
                     ARNKGALTVGGNYFNIKDRYYQTRSFSVNDTADFTRFEGLSCVVGFERNTLNRKMYAS
                     KGSYLAIRTRFVSGNEVTNPGSLQDNAPTIREWHQWWQVKMIYDNYFFRAGRYKLGFY
                     AETNFSGQPFFSNYTATILQMPGFYPTQESRTLFLDNFHAPNYLGIGVRNVFSLRSNI
                     DFRLEGYAFQPFQAVVKNDNEKTVFGDALSARYFIASFSGIFHSPVGPVAMTLNYYDR
                     KTKSFTVLFHIGYILFNKHALD"
     gene            complement(8708..9514)
                     /locus_tag="PKX59_08395"
     CDS             complement(8708..9514)
                     /locus_tag="PKX59_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOZ90924.1"
                     /translation="MLKFIDQTGFEIKLETPPQRIVSLVPSQTELLADLGLQDRIVGI
                     TKYCIYPPELFLSVPRVGGTKSVNHELINQLKPDIIIANKEENNREAIEQLRKNFPVW
                     LSDVSNLHEALDMIYNIGVMTDCQSRASEINSQIAEKFAALTIKNKKHTAKRALYFIW
                     RNPYMCVGGDTFISDMMNMAGYDNLMYYHKRYPELSEKKMAELNPEIILLSSEPYPFK
                     ARHIEEFKTICPKAEIKLVDGEFFAWYGSRMIKAADYFHQLCDSNSNVSE"
     gene            complement(9581..10057)
                     /locus_tag="PKX59_08400"
     CDS             complement(9581..10057)
                     /locus_tag="PKX59_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90925.1"
                     /translation="MRKLFMSSAICFAILLSTMVSFSQQTEKKSTESGFFVRNGLVYY
                     IKGNTVNFLESDQTLDNGATINQRGMIKYPDGKTAQLQQGEILTFNNVLVPKAVEEHF
                     ELKDGKLQIVTNGIFTPVQKAMKLETGVTIDEDGHTSSGITLMPKQKMNMMGELIK"
     gene            complement(10113..10409)
                     /locus_tag="PKX59_08405"
     CDS             complement(10113..10409)
                     /locus_tag="PKX59_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90926.1"
                     /translation="MKKLVKRLSALGAMLIISTVAFSQSTTTQETEQTTPPPTETTES
                     TEDSTAATEAVPDSTAVAPEGDPAAGAANEKPATRHKLYSGKQRGNNVIYDPKK"
BASE COUNT         3417 a         1775 c         1834 g         3469 t
ORIGIN      
        1 tcaatggggt ctttagcagc aggtattaat tcacttactt caacatcggt agtagatttc
       61 tatttcaggt catcaaaaaa agagcatagc gacagtaaga aactttacta ttctaaaatt
      121 gcaacattac tttggggaat tttttgcata ctctgtgcgt tctttgccgg caatacagga
      181 aatctgattg aagcagtaaa tatattgggg tcattattct acggcactat attgggcatt
      241 ttcatttctg caatttttgt aaaacacctt gatggcaatt ccgtttttat tgcagcttta
      301 attactgaag ctattgtaat tgcattctgg tattttgatg tgatggcatt tttatggcta
      361 aatttagccg gatgcctttt ggtagtttta attgctctgg caatacaatt ctgcacacag
      421 aatttgaaca gaatgttcaa caaaacaata tctaagtaaa gtcaattcgt atctttggca
      481 caataatttt ctcagataaa aatcatgcaa gaaataacag ttcatgaatt aaagcaatgg
      541 ctagatgaag gtaaaaactt tcagttgatt gatattcgtg aagaatatga atacgatgct
      601 tgtaatttaa acggaacgtt gataccaatg ggagaaatca tagacagaac tgaagaaatt
      661 gcaaaagaca agcctgttgt tatacattgc aggtcggggt caaggagtgc agcaatttat
      721 caagtactta gtacacaata tgggtttaca aaccttttta atttaaaagg tggtataaaa
      781 gcctattcac aagaaattga tccgacactt aacgtaatat aaattaaaac tgtggaacac
      841 ttaaaatttc taattgattt tattttacat attgataaac atttgttcga acttgtacaa
      901 cggtatgata catggatata tcttatctta tttctaattg tatttgctga aacaggattg
      961 gttgtaactc cacttttgcc aggtgattca cttttgtttg cagcaggagc tttagctgcc
     1021 ccacctgata atccgttaaa cgttttttat ctgatgataa ttttcttttt tgctgctttt
     1081 ggtggagaca acaccaacta ttttatcgga aattatatag gtccaaaagt ttttgaaaag
     1141 aactatcgtt ttctcaaaaa agattattta aacaaaacac aagaatttta tgaaaagcat
     1201 ggtggtaaag cagtaatatt tgcacgtttt gttccaatag tgcgaacatt tgctccattt
     1261 gtagccggtg ttggtagtat gacttataat cgttttattg gtttcagtgt tttagcgagt
     1321 atcttatgga ttaatatttg cgtttgggcc ggttattttt tcggcagatt agagattgtg
     1381 aaaaacaact tttcattagt tgttattgct ataatcggaa tttcattagt gcccatttta
     1441 ataggaataa tcaagcagaa attctcttct aaatgaggaa ttttggttta attggttttc
     1501 cactaagtca ttccttttca caaaaatttt ttactgataa atttaagaag ctaaacttaa
     1561 aatgtgttta caataattac gaattaagta acctcaacaa tttaagggaa ttaatcagca
     1621 caaatcagct aactggctta aatgttactt cgccatataa aacaggtgta ataccctatc
     1681 ttgaccgatt ggaagaaaca gcagaagcta ttggttcaat caatacaata ttgataaagg
     1741 ataaaaaact aattggatac aatactgaca taattggttt taaaataagt cttgagcgac
     1801 ttattgggaa tcaaaaacct ccaacattaa tattaggaac aggaggttct gcgaaaaccg
     1861 ttgcttttgt tctaaagcaa aaaaatattc catttcaatt cgtttccaga aagaaaaaag
     1921 aaggtgcaat ttcttatagt caatcagatg actttttaag tcaaaatatt ttgataatta
     1981 attgtacacc aatagggagg tttcctgatt ttggcaaaaa acctccaata aattacacca
     2041 agttaaccga caagcacttt ctatttgact taaattacaa tcctgaaatt acagcattta
     2101 tgactgaggg tataaacaaa gggtgcaagg tttgtaatgg ttatgacatg cttattgagc
     2161 aagctgaggc ctcttgggtt atctggaata cttagttatg aatacttaaa agcctgttta
     2221 gcaccattaa tcctagttaa aaaagcattg aataaacttt tgaatctttc ctgtcataag
     2281 cctatctttg ccattaaaat atcagtaaat tatgaataac attttaaaag acaacaaaac
     2341 aaaatttata acattatcaa ttctggtagt tgccatttca cgccttttac cacatcctta
     2401 taatttcact cctattggtg ctattgcact ttttgggggt acttttctta aagataagaa
     2461 gatggctttt ggtcttcctt tagcagcatt gttggtttcc gatcttttat tacaacttat
     2521 aaagccaggc agtgcatttt acgaaggatg gttttgggtg tacttaagtt ttattctaat
     2581 aacaccatta ggttttgtac tcagaaacaa attaaaagta cagaatgtag ttgcagcatc
     2641 tttaatggga tcaataatat tctttgtact gaccaacttt ggtgtatggg caacagggtt
     2701 aatgtatccg cttaccggtg caggtctaat gagttgctat gttagcgcag ttccattttt
     2761 tggaggcact ataatgggtg atttattttt caatgcacta ctttttggtg tatttgcatt
     2821 tgcaaaatgg agaatgcctg tacttgtaaa agaagcttga tagaaaattt tataatcaat
     2881 tcaaataaca tttaatgtat ttcaacaata tacttgaaac cattggtaac acgccattgg
     2941 taaaattgaa ttctgtgaca caaggagttc aggctacagt tttggctaaa atcgaaactg
     3001 taaatccagg caactcgata aaagacagaa tggcactgaa gatgatagaa gatgccgaaa
     3061 agagtggaaa gttaaaaccc ggtggaacaa tcatagaagg aactagtggt aacacaggaa
     3121 tgggattggc tattgctgca atcattaaag ggtacaaatg tatttttaca actactgata
     3181 aacaaagcaa agaaaaagta gatgcattgc gtgcttttgg tgcagaagta attgtttgcc
     3241 caactaatgt tgaaccagaa gaccctcgct cctactattc tgttgcagcc agactcagta
     3301 aagaaattcc aaatgcattt tatccaaatc agtatgacaa tttaagtaat cgtcaggcac
     3361 attatgagca aaccggtcca gaaatttggg aacagacaga agggaaaatc acacacttgg
     3421 tagttggtgc tggtacaggc ggaacgataa caggcactgg tcgttattta aaagaaaaaa
     3481 atccaaaaat taaagtatgg gcaattgaca cttatggttc agcgcttaaa aaatatcatg
     3541 aaacaggtat tttagataag aatgaaattt acccttatgt aacagagggt ataggagaag
     3601 attttatacc acagaattac gattttactg taatagatca ttttgaaaaa gttactgata
     3661 aggatgcggc attgatgaca cgtgatttgg ttcgcaaaga aggtatttgg gttggtaaca
     3721 gtgccggctc ggctgttgca gggttgcttc agatgaaaga gcaattaaaa ccaacagatg
     3781 tcgttgtaat tatttttcac gatcatggat cgaggtacat tgcaaaaata ttcaatgatg
     3841 actggatgcg atctaaaggc tatttcgata agaaaggcat gacggcatca gatattatcg
     3901 gctcaaaaaa agacgttaat ttaactactg ttgaagcaac tttaccgatt ggcgatacat
     3961 tgaaaattat aaaaacaaat aatttttctc aaatacctat cacccgtgaa ggaagaattg
     4021 ttggtaatat taccgagaat catatttact cgtcactttt aaaaagtcct gaactaaaaa
     4081 ataagccggt tagtgatatt atgcaagaag ctatgccatt tattgatatc aatacttcaa
     4141 tagatacatt aagtgacttg cttagtaaaa acaatcaggc agttattgta aaagatttta
     4201 aggctgacag aaactacatt gtaacaaggc aggatgtagt tgatgcttta gcgtaaattc
     4261 aatatttagc agaatatgtc atttggcatt tgtgaaatga gtcttgttcc tatgaggagg
     4321 gagccttcgc acagaagtga gttggtttca caaatacttt ttggtgaaca ttacgaagta
     4381 gttgaatcac aaaaagagtg gtttaaaatc agaacttcgc atgatggcta tgagggcttc
     4441 attgctttaa accaacatac agaaataagt tataacgatt ttcaatttct acaaaaaaac
     4501 ggagcatttg tttgctttga cctggtgcaa attttattac acgacaataa aattcagact
     4561 gtgattttag gtagtgcttt accatttttt gaaaatcagc attgcagaat taataccaga
     4621 acttaccggt ttgatggcaa tgcaaagttt cctcaaaaag cagctacatc agcattatta
     4681 attgaaaatg catacatgta tctcaatgcg ccatatttat ggggaggaag atctcctttt
     4741 ggtatagatt gttccggatt tacgcagttg gtttataaat tatcgggata ttctcttttg
     4801 cgcgatgctg ctttacaagc tgaacaagga aatattgtga accttgttga tgaagccatg
     4861 ccgggcgatt tagcattttt tgataatgaa gaagggaata ttattcacgt tggtatttta
     4921 actgcacgag ataccattat tcatgcatct gggaaagttc gtattgatgc atttgatcag
     4981 gtaggtattt acaatcagga aaccaaacga tacacacata aactcaggtt gataaaaaga
     5041 atattttaaa ttcatccatg aataatccgc agctcaaagc ttctgaaaat tttcctgcaa
     5101 cagtaaatga attatatgat gaagagggtg aattggtatt gattttatat actcatgaac
     5161 tggttgattt gcccgaaggc ctatggctat caacagaagg atattatgaa gcagttatta
     5221 aaaaaactga aatccaaatt gaagatgcct tgttttgcat tagtgagctc aactccatat
     5281 caggaatgca atatgagctt actttaagtt aggagagtaa tatccgattc aaattttgtt
     5341 gtaataaaat attcttctca attcttattg ctgcgtattc caacttcttt tcaattgcat
     5401 tttgaatatc aatattactc tgagtttgca tcatgaaaat tttattctct tcaagatatg
     5461 aagcaaggta ttcctgttct gtctgacccg gtgtaggtac caataatgct cttactccta
     5521 attgtgcaat atccataatt gtggaatagc ctgaccgaca aatcaccaac gtatctgtat
     5581 ttatgaactt agcaatttca tctgctggca tataattttt aaattgtaat gctcccctat
     5641 caggaatttc atttgggtcg gaaattttcc cacgtattaa taatgcacta caatggagtt
     5701 tttctaactg ttcaattatt ttaccttcaa atattgttcg ctgaggctcc ggacctgata
     5761 gaattgcaac tatatcatac ttcttatcat ttgacagatt tgaagattga aatgaagaaa
     5821 gcaatccaca attatacttt ggaattttaa taaacttatt ttcagataaa attccggaca
     5881 gtttaaatcc atgttccaca tcaggaatcc aacattcatg aaaacgattt atatattttg
     5941 catgtaatct gttaatggaa ggccgcaaaa acaacaaaga ttccggtgat tgaatcatta
     6001 gttgatgtga taaaaaaata ttctttgtaa aatttgtaaa acaaccatac ctgttatcag
     6061 aaataataat ttctgcatta tattttgaag caattttctt tatagcggat tgttcattgc
     6121 taatatttaa cagcattttt ggtgtttgtt taagcattgc ccataccata ttaccattaa
     6181 gagggtaaac aggactatat gatggtaatt cttctgcttg cagcacaggg aattgccgct
     6241 tcaatacact taagctattg ccatcagatg caataatcac tttgcaaccg gcagctaaaa
     6301 gattatcaat tacagtttta cttcttgttg cgtgacctag tccccaattt aagggagcta
     6361 caatgactgt tttactcata aaatcaatcc gaaataaatc acttaactaa tccaatgcat
     6421 gtttattaaa caatatatag ccaatatgga aaaggacagt aaatgatttt gtttttctgt
     6481 cataataatt aagtgtcatt gctacaggac ctaccggact gtggaaaatt ccggaaaatg
     6541 atgcaataaa atatctggca gataatgcat caccaaatac tgttttctca ttatcatttt
     6601 taacaactgc ctgaaatggt tgaaaagcat aaccctctaa acggaaatca atattcgaac
     6661 gtaaactaaa tacatttcga actccaatac ctaaatagtt tggtgcatga aaattgtcga
     6721 gaaataaagt tctactttcc tgagtgggat aaaatccagg catttgtaaa atagtggctg
     6781 tataattact gaagaatggt tgacctgaaa aatttgtttc agcataaaac ccgagtttat
     6841 accttcctgc tctgaaaaaa taattatcgt aaatcatttt aacctgccac cattgatgcc
     6901 actctctgat agttggggca ttatcttgta aagaaccggg attggttact tcattgccgg
     6961 aaacaaaacg tgttcgaata gctaagtaag aaccttttga tgcatacatc ttcctattta
     7021 aagtgtttct ttcaaaacca acaacacaac ttaatccctc aaacctggta aagtctgcag
     7081 tatcattaac agagaaactt ctggtttgat aatacctgtc tttaatgtta aaataatttc
     7141 cacctacagt tagtgctcct ttatttcgtg ccggcatacc aatatttaat ccataggact
     7201 ggtctgttac taataaatat gcaggtttga tatcatcttt aaaaaaacta ctgcttttaa
     7261 aatagtccat cctgtttaaa gtagtaaacg gctcaataaa aaagtcaggt ctgccgggta
     7321 tatcgagcct caatttaagc tgaccggaat tatatagttt tccgaaatag gtattggcat
     7381 ggatacttaa actatatgga ccaagaatat tatatcggaa gccaataaac ccttcactaa
     7441 ttggtcgtga agagaaattt cctcctatat caactcgcaa atctgtttcc ggtttaatat
     7501 aaatctccag attatatact tctgttttag gatcataaac caattttgga aatgcatatc
     7561 ttatattttc atctgcaagc aatttaaaat aacggggttt gagttgtcga agtagcatat
     7621 tgcttccgcg aggattcaaa atagacttaa tatattttgt tttttcgggg cttacacctg
     7681 ttactacaat gtcgccaact attaaaggat gtaatttgct aataaagtta tctcgtttct
     7741 gggttaattc ttcgggtgag attcttctgg caatagcttg tttgatatca tccattcgag
     7801 ccattgttgc tttataacca ctatcaatca aagcttgaaa attataaaaa tcaaatactc
     7861 caatattggt aacatcaggt tgaattaaaa tatcatcagc tccgggcaat acaacatgct
     7921 gcgattgaat tatcattgtt ctgagttgtg aaaacatatt gtcttctttc ggaatatcag
     7981 ggccaccgcc cactgaaaca ccaatcataa tatcaggatc aaactcattg agcataacat
     8041 ctgagggaaa gttattataa attccaccat cgtagtaaag ctgattatcg gctgtcatag
     8101 gtgagagata cagcggatag gcaattgagg ctcttattgc ctgactaagg tcgccacaat
     8161 taaaaattac cgggcgtttg tttttaatat ctgaagcaac acaacgaaat ggaacaaaga
     8221 ggttattaaa atcataatcg gcaccggcac ttgcagctga atgattttca agcagtgcaa
     8281 agtctatttg tccactattg accaatgctc cattaagatt cattcttaaa gttgaatcat
     8341 agctaagttt aacagttgcc catgatgcat ctctttcgcg ttccttgaaa aaatatataa
     8401 gttcatcgct caaggtgcct gttgcccaat ttttaaaatc gtcacttaaa acaatatttt
     8461 gcatttgttg tgggctggta cctgaaacat acatgcctcc aacaagagct cctgctgaag
     8521 ttcctgtaat aaaatcaata ggaattccat tttcttccaa tgcttttatc acgccaatat
     8581 gggcaaatcc tcttgcaccg cctccactta ataccaaacc aactttttgc gcaaatagaa
     8641 tatttacatt taataaacta attatagata acacctttat ccatttctgc atcatcgtgc
     8701 aaaggtatta ttctgacaca tttgaattac tgtcgcatag ttgatggaaa taatcagcag
     8761 cttttatcat acggctgcca taccatgcaa aaaattcacc atcaaccaat tttatctcag
     8821 ctttaggaca gatcgtttta aattcttcaa tatggcgtgc tttaaaagga tatggctctg
     8881 aagaaagcag aataatttca ggattaagtt ctgccatttt cttttctgaa agctcaggat
     8941 atcttttgtg atagtacatc agattatcat agccggccat gttcatcata tcgcttataa
     9001 atgtatcacc tcccacacac atataggggt ttctccaaat aaaatacaat gctctttttg
     9061 ctgtatgttt tttatttttg atggtaagag cagcaaattt ttccgcaatc tgactattga
     9121 tttctgaagc gcgactctga caatcggtca ttacacctat attataaatc atgtccaatg
     9181 cttcgtgaag attactaaca tcacttaacc agaccggaaa attttttctt agttgctcaa
     9241 tagcttcgcg attgttttct tctttattag ctataataat atcgggcttc agttggttga
     9301 ttaactcatg attcacagat tttgttccac cgacccgagg tacagataaa aaaagttcgg
     9361 gtggataaat gcaatatttt gtaataccaa ctattctatc ttgtagtcct aaatctgcaa
     9421 gcaactctgt ttgtgatggg actaaggaaa caattctttg aggtggtgtt tccaatttaa
     9481 tttcaaatcc tgtttgatct ataaacttca acatgtcagg taaagttaag cataaaaaaa
     9541 gccggtatta cccggctttt acatattttt taaaccaaca ctattttatt aattctccca
     9601 tcatgttcat tttttgtttt ggcataaggg ttattcctga tgatgtatga ccatcttcat
     9661 caattgtgac accggtttcc aatttcatgg ctttctgtac aggagtaaaa atgccattag
     9721 ttacaatttg aagcttacca tcttttaatt caaaatgttc ttcaactgcc ttcggaacaa
     9781 gaacgttatt gaatgttaaa atctctccct gttgtaactg cgctgtcttt ccatcgggat
     9841 attttatcat tccacgttga ttaatcgttg caccattatc aagtgtttga tcgctctcca
     9901 gaaaatttac tgtgttgcct ttaatataat agacaaggcc attgcgaaca aagaaacccg
     9961 attctgttga ttttttttca gtctgttgac tgaaagacac cattgtgctt agtaaaattg
    10021 caaagcaaat agctgaactc atgaataact ttctcattga tttttatttt ataaaagtaa
    10081 aattacgatg aaataaatta aaaaacaacc ccttactttt taggatcgta aattacattg
    10141 ttgccacgtt gcttgccaga gtacaacttg tgtctggtag caggtttttc attagctgca
    10201 ccggcagcag gatcaccttc gggagcaact gcagttgaat cgggtacagc ctcagtagct
    10261 gctgtggagt cttctgtgct ttctgtagtt tctgtaggtg gtggtgtagt ttgttctgtt
    10321 tcctgtgtag tagttgactg cgaaaatgct acagttgaga taatcaacat tgcaccaagg
    10381 gcagacaagc gtttaactaa ctttttcatt gtattatttt tttccaaaat taataatcaa
    10441 aacgatgatt caacaatcat attttatttt tattgcattc agatgttaat aaatc
//
LOCUS       DAOOOI010000069        10346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1269_length_10346_cov_14.429307, whole genome shotgun
            sequence.
ACCESSION   DAOOOI010000069 DAOOOI010000000
VERSION     DAOOOI010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10346
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1269_length_10346_cov_14.429307"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKX59_08410"
     CDS             complement(<1..211)
                     /locus_tag="PKX59_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90927.1"
                     /translation="MLQADSIVVKDATKLSENNSPQTTRILFPFNPNNLADSLWTKLG
                     LSEKQIASIKKYEERGGKFRIRSDVK"
     gene            551..907
                     /locus_tag="PKX59_08415"
     CDS             551..907
                     /locus_tag="PKX59_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HOZ90928.1"
                     /translation="MSVLPPCPLCHSIYTYENGNLMVCPECFYEWNPEEQQSVSEETT
                     SADSGKVFDANGNELQNGDAVIVIKDLPVKGAPKPVKAGTKVKNIRLVEGNHNIDCKI
                     DGFGSMALKSEFVRKA"
     gene            complement(915..1826)
                     /locus_tag="PKX59_08420"
     CDS             complement(915..1826)
                     /locus_tag="PKX59_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOZ90929.1"
                     /translation="MIFPLPLKAGDTIAIVATARKVSRDEMAPAIDILQQWGLHVTEG
                     KNLYADKNQFAGDDTLRAEDFQWALDNPNIKAVLFARGGYGSVRIIDSINFSEFKKNP
                     KWLIGFSDITTIHSHVHKQLNIVTLHAPMAINFPKSPNVILKQIKDFLFGNSIVLNAA
                     THPLNRTGNATGQLVGGNLSILYSLAASASDIDTNGKILFLEDLDEYLYHIDRMMMQL
                     KRSGKLHGLAGLVVGAMSDMKDNTIPFGKTAEEIIAEHVSEYNFPVCFNFPAGHITEN
                     NPLAMGSYAELIVENKVSLKMNHKILS"
     gene            1964..4057
                     /gene="metG"
                     /locus_tag="PKX59_08425"
     CDS             1964..4057
                     /gene="metG"
                     /locus_tag="PKX59_08425"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOZ90930.1"
                     /translation="MSRYLITAALPYANGPVHIGHLAGCYIPADTYSRYLRLKGEDVK
                     FICGSDEHGVPITIKARQLGITPQQVVDKYHQMMKSSFEKLGISFDHYSRTSSPIHHQ
                     TASDFFKTLYDKGVFIEETTEQFYDEEAKQFLADRYITGTCPRCNNPNAYGDQCENCG
                     ASLNPTDLINPKSKLSGNTPVLRPTKNWFLPMGEIQKTKEFQEYIKRFDNWKPNIKGQ
                     CSSWLNEGLHPRAMTRDLDWGVKVPLPNTEGKVLYVWFDAPIGYISATKEFLPNNWKD
                     YWQKQNKEEENPKLIHFIGKDNIVFHAIIFPMMLMQHGGFVLPDNVPANEFLNLEGDK
                     ISTSRNWAVWLHEYLEDFPGREDELRYVLTSIAPETKDSEFTWKDFQARVNNELGAIV
                     GNFVNRVVVLSNKYFKGNLTTDREIAASPFENELKKLLQSYTKAMENFQFRDAQSIAM
                     DFARIGNKYLTECEPWKKYKADDVTSVNQTREVLIHCLSLVSKMAVLFQPFLPNASAK
                     MLDMLKVDSEKRKLTKNGFEKNKISIKKIFINFDSLILFQKIEDADIEKQIQKLHDKK
                     VMNTEQNQQAEVVSETTIAPQLAETTFDDFSKMDIRVATILEAERVPKTDKLLKLLLD
                     TGIDKRTVVSGIAAWYDPEKIIGQKVVMLANLAPRKIKGIESKGMILMAQNDKGELSF
                     ITPSKPQMDNGNTVK"
     gene            4084..5478
                     /gene="fumC"
                     /locus_tag="PKX59_08430"
     CDS             4084..5478
                     /gene="fumC"
                     /locus_tag="PKX59_08430"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOZ90931.1"
                     /translation="MEFRKEKDTMGIVEVPANVYWGAQTQRSIENFKIARDTNKMPKE
                     IIEAFAYLKKAAAQTNFEAGVLPKEKNELIARVCEEILEGKLNDQFPLVVWQTGSGTQ
                     SNMNVNEVVAYRAHVINGGSLLDEKKAIHPNDDVNKSQSSNDTFPTAMHIAAYKILID
                     VTLPGIKKLRDTLKAKSKEFMNVVKIGRTHLMDATPLTLGQELSGYVSQLDHGIRAIE
                     HTLPHLAELALGGTAVGTGINTPKGYDVNVAKHIASLTRLPFVTAENKFEALAAHDAI
                     VEAHGALKTVACSLMKIANDIRLLASGPRCGIGEVYIPDNEPGSSIMPGKVNPTQCEA
                     MTMVAAQVLGNDVAINIGGATGHFELNVFKPVMIYNFLHSARLIGEVCISFNDKCAIG
                     IAPLHDNIKKNLENSLMLVTALNTHIGYYKAAEIAQTAHKQGKTLKQTAIDLGYVTSE
                     QFDAWVKPEDMVGL"
     gene            5511..5924
                     /locus_tag="PKX59_08435"
     CDS             5511..5924
                     /locus_tag="PKX59_08435"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOZ90932.1"
                     /translation="MKKLIIFAFIFLTSVVAFAQSGDVFTTVEKMPEFPGGQTGLQEY
                     LLKNIKYPEEARTNVVEGVSYINFVVSSTGDVTGARVLKGNNEQFNQEALRVVSAMPR
                     WQPGMQGGQTVSVQYTLPVRFSLTVPDKEGKKHKK"
     gene            6015..6851
                     /locus_tag="PKX59_08440"
     CDS             6015..6851
                     /locus_tag="PKX59_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ90933.1"
                     /translation="MNRFKNILINGKHGKPIVTDMFFNGNKEPMPVIIFAHGFKGFKD
                     WGPYDMIAESFAHSGFVFIKFNFSFNGTTPDNLTEFADLEAFGQNNFTKELDDLDTLL
                     NWLWQHPHKERFDLNTIYLAGHSRGGAISIIKAAEDGRISKIASWASPIEFSKYISPQ
                     QLSLWQQAGVIYIENARTHQQMPLYYQLAEDAINNAQRINIHKAVKQLSIPHLIIHGT
                     EDETVSFSDALVMHSWNTRACLTPITSANHSFGAEHPYAGQLLPPHYQQVVNETIAFF
                     RG"
     gene            6936..9032
                     /locus_tag="PKX59_08445"
     CDS             6936..9032
                     /locus_tag="PKX59_08445"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOZ90934.1"
                     /translation="MAIIGKIRSKAGLLIGIIGLSLVAFILGDLLTSNRSFIQGNTTT
                     AGIIGGKKISIQDFEAKVQQNIENYKLSQGKDQIDQQTTDQLRDQTWGQMLNEELIGK
                     QIAKTGLVVSSDELFDMVQGNDIHPQIKQAFSDPNTKQFDKKNVIQFLKNMDNDQTGR
                     TRAQWVNFENSIKEERLNQKYFDLIKKGLYVTTAEAKLDFEAKNKTESARYIMLNYST
                     IADSTVKLTDADLQKAYNENIKSYEQPEESRKLDYVTFDVVPSNEDRAVAEKYITDLV
                     PAFTASTDDSLFVAQNSDFPVDNSYHKTGTLSPAIEAQMFAEAVGFVSAPYMENNAYY
                     VAKLEGSKMLPDSVQARHILFKTEGKDSLHVKATADSIYNLIKGGAKFDQFAMLSEDP
                     GSAIKGGDLGWFKPGMMVPEFNDFCFDNKKGSLGVVKTNFGYHIIEITDQAGMSKNVR
                     VYAVGRKIEASSKTYQGVFAKANDFAAKNNNGDSYTKAVKDEGLNARNIESLKENDKT
                     VPGLENARELIRWAFKAKKNDVSTPFELGDKFVVAHLMQIKPEGTLALEDVKEQVEVE
                     ARKNKKAEMLIEKINATGSAQSLDGLASKLNIAVQPLENVTFNSPYIPNLGAEMNVIG
                     TMFGIGKDKVSKPIKGEQGVFVVQVTDVKEPQAPANFNESKTQAEQQLQNRSQYETFN
                     ALKEKAKVKDYRGKFY"
     gene            complement(9249..9941)
                     /gene="purQ"
                     /locus_tag="PKX59_08450"
     CDS             complement(9249..9941)
                     /gene="purQ"
                     /locus_tag="PKX59_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOZ90935.1"
                     /translation="MKFGVVVFPGSNCDEDMVYVLRNILKQPVEKLWHKEHSLRGCDF
                     IVLPGGFSYGDYLRSGAIARFSPIMQEVMEFAAKGGYVLGVCNGFQILCESQLLPGAL
                     LHNNSHKFICKNVFIKAETDETILTSSLKIGSAIKIPIAHGEGRFFADEATVNQIESN
                     NQVLFRYCDENGYVTEAANPNGAINNIAGICNEKRNVFGMMPHPERAADTLLGNTDGL
                     GIFKSILNYVDA"
     gene            complement(10002..>10346)
                     /locus_tag="PKX59_08455"
     CDS             complement(10002..>10346)
                     /locus_tag="PKX59_08455"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HOZ90936.1"
                     /translation="NAPDNLLQVIFRMQVLGYKPIMAHPERYPFWGNNFDFYGSLREQ
                     GVLLQINVNSLGGYYGPDAKRIAEKLIEKEWVDLIGTDTHATKHIQALHKTVREKSFR
                     KLLEFNLLNKHL"
BASE COUNT         3416 a         1881 c         2059 g         2990 t
ORIGIN      
        1 tttttacatc tgaccggatt ctgaattttc ctccacgctc ttcatatttt ttgattgatg
       61 caatttgttt ttctgataaa cctaacttgg tccaaagcga atcggctaaa ttatttggat
      121 tgaaagggaa aagtattcta gtagtttgtg gagaattatt ttctgaaagt ttcgttgcgt
      181 ctttgacaac aatagaatcg gcctgtaaca aattccattg tgctacagca gaatctaaac
      241 gacttgtgtt gatggcaatt ggtggctcat aaaatttaag tccgtacagt gtcagcagca
      301 gtacaacaat aataatggat aatacgataa tgcccttacg ttcactgtaa gtaaacgtgt
      361 tgtactgttt aaaaactccg taggttatga aaaggcggtt tctttttcat ggttaaaacg
      421 gtaagttaat tttctaaagc gaagatagta ttttaaaaca gagtttgtat gcaatggatt
      481 tcattttctt gaagcctgat tgattataat gtgggggttt ttaaagatat ttgcagaaaa
      541 tatttttatc atgtctgtct tgccgccatg tcctttatgt cattcaattt atacctatga
      601 aaatggtaat ctgatggttt gtcctgaatg tttttacgag tggaatcccg aagaacaaca
      661 atctgtttca gaagaaacga caagtgctga tagtggaaaa gtttttgatg ctaacggaaa
      721 tgaattgcaa aatggcgatg cagtaattgt cattaaagat cttcctgtaa aaggagcacc
      781 caaacctgtg aaagccggca ccaaggttaa gaacattcgt ctggtagaag gcaatcataa
      841 cattgattgc aaaatagatg gctttggctc aatggcactt aaatcggaat ttgtaaggaa
      901 ggcataaaat aaaatcagct aaggatttta tgattcattt tcagtgacac tttattttct
      961 acaataagtt ctgcatagct tcccattgcc agtggattat tttctgtaat atgtcctgca
     1021 gggaaattaa aacaaacagg aaaattgtat tctgaaacat gttcggcaat aatttcttct
     1081 gctgtttttc caaatggaat ggtgttgtct ttcatatcgc tcatagcacc aacaacaagt
     1141 cctgctaaac catgtaactt accactgcgt ttgagctgca tcatcatacg gtcaatatgg
     1201 taaaggtatt catccaaatc ttccaaaaaa agaatttttc cattagtatc aatgtcagat
     1261 gctgatgcag caagtgaata taaaatagaa agattgcctc caaccaattg cccagtggca
     1321 tttcctgttc tgtttaaagg atgtgtggca gcatttaaca caatgctatt gccgaaaaga
     1381 aaatctttta tttgttttaa aataacgtta ggcgatttcg gaaaatttat tgccataggt
     1441 gcatgcagtg tgacaatgtt caactgcttg tgcacatgtg agtgaatggt agtgatatcg
     1501 ctgaagccaa taagccactt tggatttttt ttgaactctg aaaaattaat gctgtcaatt
     1561 attcttactg agccataacc accacgtgca aaaagaacag ctttaatgtt tggattatcc
     1621 aatgcccact gaaaatcttc tgcacgtaaa gtgtcatcac cggcaaactg atttttatca
     1681 gcatataaat ttttaccctc tgtaacatgt aatccccatt gctgaagtat gtcaattgcc
     1741 ggagccattt catcgcggct gactttgcgt gctgtggcca caatggcaat ggtatcacct
     1801 gctttaagcg gtagggggaa aatcatctgc tgttgttttt ttatctttgc aaagtaaaaa
     1861 gaaaagagtt tttagttaga tgttttgcgt ttaaagtttt taaaaaacct tccggacttt
     1921 agctttttaa ctttctagac tttacaactt ttaaaaacaa ttcatgtcaa gatatttaat
     1981 cactgccgca cttccctatg ccaacggtcc ggttcatata gggcaccttg ccggatgcta
     2041 tattcctgcc gatacctatt cgcgctattt gcgacttaaa ggcgaagacg taaaatttat
     2101 ttgtggaagt gatgagcatg gcgtacctat tactatcaaa gcacgacaac ttggtattac
     2161 accacagcaa gtggttgaca aataccatca gatgatgaaa tcgtcatttg aaaaattggg
     2221 catttcattc gatcattatt cacgcacatc ttctcctatt catcatcaga cagcatccga
     2281 tttttttaaa acactttatg acaaaggcgt ttttattgaa gaaactacag agcagtttta
     2341 tgacgaagaa gcaaaacaat ttttagcaga ccgttatatt accggtactt gtccgcgttg
     2401 taataatcca aatgcctatg gcgaccaatg cgagaactgc ggtgcttcac tgaacccaac
     2461 agaccttatc aatccaaaat caaaactgag tggcaacaca cccgttctca gaccaacaaa
     2521 aaactggttt ttgccaatgg gagaaattca gaagacaaaa gaatttcagg aatacattaa
     2581 acgcttcgac aactggaagc caaacattaa aggacaatgt tcaagctggc ttaacgaagg
     2641 attgcatccg cgtgcaatga cacgcgacct cgactggggt gtgaaagtac ctttgcccaa
     2701 tacagaggga aaagtgctat atgtgtggtt tgatgcacct ataggatata tatctgcaac
     2761 aaaagagttt ttaccaaaca actggaaaga ttattggcaa aagcaaaaca aagaagaaga
     2821 aaatcctaag ctcattcatt ttatcggtaa ggacaatatt gtttttcatg ccattatttt
     2881 tccgatgatg ttgatgcagc atggcggctt tgtattgcct gataatgttc ctgcaaatga
     2941 gtttttgaat cttgaaggtg ataaaatttc tacttcgcgc aactgggcag tgtggctgca
     3001 cgaatatctt gaagattttc cgggtcgtga agatgaattg cgttatgtgc tgacatctat
     3061 tgcaccggaa actaaggaca gtgagtttac ctggaaagat tttcaggcta gagtaaataa
     3121 tgaactggga gctattgtag ggaattttgt aaacagagtt gtggtgttga gtaataagta
     3181 ttttaaaggc aacctcacaa ctgatagaga aattgctgct tcaccatttg aaaatgaatt
     3241 gaaaaaatta ttgcagtctt atactaaagc aatggaaaat tttcaattca gagatgcaca
     3301 gtcaatagca atggattttg ctcgaatagg taataagtat ctgacagagt gtgaaccatg
     3361 gaaaaaatat aaagcagatg atgttaccag tgtcaatcaa accagagaag tactgattca
     3421 ttgcctatca ttggtttcaa aaatggctgt gttgtttcaa ccttttttgc caaatgcatc
     3481 agctaaaatg cttgatatgc ttaaggtaga ttctgaaaaa agaaagctta ccaaaaatgg
     3541 ttttgaaaaa aataaaattt ctataaaaaa aatatttata aattttgaca gccttatctt
     3601 atttcaaaaa atagaagacg ctgacattga aaaacaaatt caaaaacttc atgacaaaaa
     3661 agtgatgaat acagaacaga atcagcaagc agaagttgtt agtgaaacaa ccattgcgcc
     3721 tcaacttgcc gaaactactt ttgatgattt cagcaagatg gatattcgtg ttgcaacaat
     3781 tttagaggca gagcgcgttc caaaaacgga taaacttttg aagttactac tcgacacagg
     3841 cattgataaa cgcactgttg tttcaggtat tgcagcatgg tacgatcccg aaaaaattat
     3901 cggacaaaaa gttgtgatgt tagccaacct tgctccacgc aaaattaaag gtattgaaag
     3961 caaaggaatg atactgatgg cacagaatga taaaggagag ctttcattta ttacaccatc
     4021 aaagccgcag atggacaatg gaaacaccgt taaatagatt taaaatttat taattaaaat
     4081 acaatggaat tcagaaaaga aaaagacacg atgggaattg ttgaagttcc tgcaaacgtt
     4141 tattggggtg cacaaacaca gcgctcaata gaaaatttta aaatagcccg cgacacaaat
     4201 aaaatgccta aagaaattat tgaagcattt gcctatctta aaaaagctgc tgcacaaacg
     4261 aattttgaag caggtgtttt accaaaagaa aaaaatgaat tgatagcacg agtgtgcgaa
     4321 gaaatcctcg aaggaaaatt aaacgatcag tttccacttg ttgtatggca aacaggtagt
     4381 ggtacgcaaa gtaacatgaa tgttaatgaa gttgtagcct accgcgcaca tgtcattaat
     4441 ggtggaagtt tattggacga gaagaaagct attcatccaa atgatgacgt aaacaaatcg
     4501 cagtcaagca acgatacttt tccaacagca atgcatattg ctgcctataa aatattgatt
     4561 gacgtaacac ttccgggaat taaaaaactg agagatacac tcaaagccaa gtcgaaggag
     4621 tttatgaatg tggtaaaaat cggacgtaca cacctgatgg atgccacacc gcttacatta
     4681 ggacaggaat taagtggcta tgtatcacaa ctcgatcacg gtattcgtgc tattgaacat
     4741 acactaccgc atttagcaga acttgcattg ggtggcactg ctgtcggtac aggaatcaac
     4801 acacccaaag gttatgatgt gaatgtagcc aagcacattg catcattaac aaggctgccg
     4861 tttgttactg ccgaaaataa atttgaagca ttggcagcac acgatgccat tgttgaagca
     4921 catggtgcct taaaaactgt tgcctgctca ctgatgaaaa tagccaacga cattcgtttg
     4981 cttgccagcg gcccccgttg tggcattggc gaagtatata ttcccgacaa cgagccgggt
     5041 tcatctatca tgccgggaaa agtaaatcct acgcagtgcg aagccatgac aatggtagct
     5101 gcacaggtgt tgggtaacga tgtagccata aacattggtg gtgcaacagg acattttgaa
     5161 ctgaatgttt ttaagccggt gatgatttat aacttcctgc atagtgcacg attgattgga
     5221 gaagtttgta tttcgttcaa cgacaaatgc gccataggca ttgcaccatt acatgacaat
     5281 atcaaaaaaa atctggaaaa ttcattaatg ctggtaactg cacttaacac acacattggt
     5341 tactacaaag cagcagagat tgcacaaaca gcacataaac aaggcaaaac tttaaaacag
     5401 acagctattg atttgggtta tgttacttca gagcagtttg atgcctgggt gaagcccgaa
     5461 gatatggtgg gattgtaatt tttattactt ttacgattaa ataatagttt atgaagaagc
     5521 ttatcatttt cgcatttatt tttctgactt cggttgtggc atttgcgcag tcgggtgatg
     5581 tgtttaccac agtagaaaaa atgcctgaat ttcccggagg tcaaacagga ttgcaggaat
     5641 atcttcttaa aaacatcaaa tatcccgaag aggcaaggac caatgttgtg gaaggcgttt
     5701 cgtatataaa ctttgtggtg tcatcaacag gagatgttac cggtgcgcgt gttttaaaag
     5761 ggaacaatga gcaatttaat caggaagctt tacgtgtagt tagtgctatg cctcgctggc
     5821 aacccggcat gcagggtggg cagactgtaa gcgtacaata tacattgcct gtacgttttt
     5881 cattaacggt gcctgataaa gaaggtaaaa aacataaaaa atagattaat atcatctttt
     5941 tttgaaacag ctgtcaaaac ttcattgaca gctgttttgt ttttttgtaa attgtgctct
     6001 catattttaa tacaatgaac cgctttaaaa atatcctcat taatggtaaa catggtaaac
     6061 cgattgtaac cgacatgttt ttcaatggca ataaagagcc aatgcctgta attatttttg
     6121 ctcatggctt taaaggcttc aaagactggg ggccttatga tatgattgca gaatcgtttg
     6181 ctcattcagg atttgtcttt attaaattta atttttcgtt taatggaact acccccgata
     6241 acctgactga atttgcagac cttgaagcat tcggacaaaa taattttacc aaagaacttg
     6301 atgatttaga cacactactc aactggcttt ggcagcatcc acataaagag cgttttgatt
     6361 taaacaccat ttatctggca ggacactctc gcggaggagc tatttcaata attaaagctg
     6421 ctgaagatgg tcgcatcagc aaaattgcat catgggcatc gcctattgag tttagtaaat
     6481 atatttcgcc acagcaactt agtttatggc agcaggcggg agtcatttat atagaaaatg
     6541 cacgaaccca tcagcaaatg ccactctact atcaacttgc tgaagatgcc atcaacaatg
     6601 cacaaagaat aaatattcat aaggcagtaa aacagctttc aatacctcat ttaatcattc
     6661 atggaacaga ggatgaaaca gtaagtttta gtgatgcctt agtcatgcat tcatggaata
     6721 caagggcctg tctgacacca ataacatcgg caaaccatag ttttggtgcc gagcatcctt
     6781 atgccggaca attactgcct cctcattacc agcaagttgt aaacgaaacc attgcttttt
     6841 tcagaggctg atatctctaa agatttcgtt ttatttattg cattttcagt tgtatattcg
     6901 ccccctcaat tttaaacccc cattattaag acattatggc tattattgga aaaataagaa
     6961 gtaaggccgg cctgctcatt ggtattatcg gattaagctt agtagctttt atccttggcg
     7021 acttgctcac ttctaaccgc tcattcattc agggaaatac cacaacagca ggtatcatag
     7081 gtggaaagaa aatctctatt caggattttg aagccaaagt ccaacagaat atagaaaatt
     7141 ataaactcag tcagggtaaa gatcagatag accaacagac tacagatcaa ttgcgcgatc
     7201 aaacctgggg acagatgctt aacgaagaat taatcggcaa gcaaattgca aaaacaggtt
     7261 tggtagtgag cagcgatgag ttgtttgaca tggttcaggg taatgacatt cacccacaga
     7321 taaaacaggc attcagcgac ccaaacacca agcagtttga taagaaaaat gtaatccagt
     7381 ttttaaaaaa catggataac gatcagacag gccgcaccag agcacaatgg gtaaacttcg
     7441 aaaacagcat taaagaagaa agactgaatc agaaatattt cgacctgatt aaaaaaggcc
     7501 tttatgttac cactgctgaa gcaaagctcg actttgaagc aaagaacaaa actgagtctg
     7561 cacgttacat catgcttaac tacagtacca ttgccgatag cacagtgaaa cttactgatg
     7621 ccgatttgca aaaagcatat aacgaaaata ttaaaagcta tgagcagccg gaagaatcgc
     7681 gcaaactcga ctatgtgact tttgatgttg tgccttctaa cgaagacaga gcagtagcag
     7741 aaaaatatat tacagacctt gttcctgcat ttactgcaag cacagacgac tccttgtttg
     7801 tagctcagaa ctcagatttt cctgttgaca acagctacca taaaacagga acactttctc
     7861 ctgctattga agcacagatg tttgcagaag ctgtaggttt tgtgtcagca ccatatatgg
     7921 aaaacaatgc ctactatgtt gcaaaattag aaggctcaaa aatgcttcct gattctgttc
     7981 aggcacgcca tattttattt aagacagaag gaaaagattc attacatgtt aaagcaactg
     8041 ccgacagtat ttacaatctg ataaaaggcg gtgctaaatt cgatcagttt gctatgcttt
     8101 ctgaagatcc gggctctgct attaaaggtg gcgacttggg ttggtttaaa cccggaatga
     8161 tggttcctga attcaacgac ttttgctttg acaataaaaa aggctcgtta ggtgtagtaa
     8221 aaacaaactt cggttatcat ataattgaaa ttaccgatca ggcaggtatg tcaaaaaatg
     8281 taagggttta tgctgttgga cgtaaaattg aagccagctc taaaacctat cagggtgtat
     8341 ttgcaaaggc aaatgacttt gcagcaaaaa acaacaatgg cgacagttat actaaagctg
     8401 taaaagacga aggattaaat gcccgtaata ttgaaagctt aaaagaaaat gacaagactg
     8461 ttccaggcct tgaaaatgca cgtgagttaa ttcgctgggc atttaaggct aagaagaacg
     8521 atgtttcaac accatttgag ttgggtgata aatttgttgt agcacattta atgcagataa
     8581 aacctgaagg cacattggca ttggaagatg taaaagaaca ggtagaagtt gaggcacgca
     8641 aaaacaaaaa agcagaaatg ctgattgaga aaattaatgc aacaggttca gcacaaagtc
     8701 ttgacggatt ggcatcaaag ctaaacatcg ctgttcagcc tttggaaaat gtaacattta
     8761 actctccata cattccaaac ttaggtgcag aaatgaatgt tataggaact atgtttggaa
     8821 taggaaaaga caaagtgagc aagccaatta aaggagagca aggtgtattc gttgttcagg
     8881 ttactgatgt aaaagaaccg caggctcctg ctaattttaa tgaaagtaaa acgcaggcag
     8941 agcaacaatt gcaaaaccgt tcgcagtacg aaacctttaa tgcattgaaa gaaaaggcaa
     9001 aggttaaaga ttatagaggt aagttctatt aagaattgta aaaaagattt tacttaaaaa
     9061 aataagggct tcggaacaaa aatctccgaa gcccttaata tttttggggt gtgaataaag
     9121 agaaagtcat attgtggttt aaaaggctgg ggttggcagg atttttattt ttcctgatca
     9181 aaggccttat ctggcttgca gtactttatg gtgcagtaga gtggtttaaa taattgtgct
     9241 taatatagtc aggcatcaac atagttgaga atagacttaa aaattccaag tccatctgta
     9301 tttcccaata atgtgtctgc ggcacgctca ggatgtggca tcattccaaa cacatttcgt
     9361 ttctcattac agatacctgc aatattgttt atggcaccat taggatttgc agcttccgtt
     9421 acatagccat tctcatcaca atagcgaaaa agtacctgat tgttcgattc tatctgattt
     9481 actgttgctt catctgcaaa gaaacgtcct tcgccatggg ctataggaat tttaatggct
     9541 gagcctattt tcagtgatga tgtaaggatg gtttcatcag tctctgcttt tataaaaaca
     9601 tttttacaga taaatttatg gctgttgttg tgcaataatg ctccaggcag cagttgcgat
     9661 tcgcataaaa tctgaaagcc attacaaaca cctaaaacat aaccaccttt tgcagcaaac
     9721 tccatcactt cctgcataat gggcgaaaaa cgtgcaatag cacccgaacg caaatagtca
     9781 ccataagaaa agcctccggg aagtacaata aaatcgcatc ctcgaagtga atgttcctta
     9841 tgccataatt tttctaccgg ttgtttcaat atgttgcgca acacatatac catgtcctcg
     9901 tcacaattgg aacccggaaa taccacaaca ccaaatttca tgattgattt ttttactgac
     9961 tacaaaagta acgataaatt aaactgcttt aacaatcttt ttcagagatg tttgttgagc
    10021 aaattaaact caagaagttt cctgaatgat ttttcacgta cggttttatg caatgcctga
    10081 atgtgtttgg tggcatgtgt gtcggtgcca ataaggtcaa cccattcttt ttctataagt
    10141 ttttctgcaa tacgtttggc atcgggacca tagtacccgc caagagagtt tacatttatc
    10201 tgtaacaaaa caccttgttc gcgcagactg ccatagaagt cgaaattatt tccccagaaa
    10261 ggataacgtt ccggatgtgc cataatgggt ttgtagccca gcacctgcat tctgaaaatt
    10321 acctgtaata aattatcggg agcgtt
//
LOCUS       DAOOOI010000070        10254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1275_length_10254_cov_12.022257, whole genome shotgun
            sequence.
ACCESSION   DAOOOI010000070 DAOOOI010000000
VERSION     DAOOOI010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10254
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1275_length_10254_cov_12.022257"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..416
                     /locus_tag="PKX59_08460"
     CDS             57..416
                     /locus_tag="PKX59_08460"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOZ90937.1"
                     /translation="MKTSDSFNADEISFFEKVYQVVRLIPKGKVTSYGAIAKYLGSAQ
                     SSRMVGWAMNNSSRIKPKVPAHRVVNRNGMLTGKHFFGANDAMQHLLEKEGIKVSDDK
                     IIDFKKHFWDPSVELML"
     gene            447..1547
                     /locus_tag="PKX59_08465"
     CDS             447..1547
                     /locus_tag="PKX59_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOZ90938.1"
                     /translation="MQITKEKVLEALSYVQEPDLKKDLVTLNMIKDIEIEGQNVSFTV
                     ILTTPACPLKAEIERACKNAIVHFVDKDAVVTVNMTANVTTQRSMNNEMMKGVKNIIA
                     VASGKGGVGKSTVAANLAVSLAKTGAKVALVDADIFGPSVPIMFGSEHEHPFIEEVNG
                     RQLMIPVEKHGVKLISIGYLADPSQAIVWRGPMASKALKQLFSDVNWGEIDYMIVDLP
                     PGTSDIHLTLISAVPLTGAVVVTTPQDVALADARKAIAMFQLPSVNIPVFGLIENMSW
                     FTPEELPDNKYYLFGKDGGQKLAAEMNVKLLGQVPLVQSVREGGDIGNPIATGNSIVS
                     EAFNELSKSVAQQVAIRNAVIEPAQRVPAAGF"
     gene            1559..1819
                     /locus_tag="PKX59_08470"
     CDS             1559..1819
                     /locus_tag="PKX59_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOZ90939.1"
                     /translation="MSESLKNNLKHKVEQALDTIRPYLKSDNGDVTVLDITPQNVVKL
                     RFEGACSSCTMSAMTFKAGVEETILRMVPEITAVEALNVENV"
     gene            1885..2604
                     /locus_tag="PKX59_08475"
     CDS             1885..2604
                     /locus_tag="PKX59_08475"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90940.1"
                     /translation="MKITAAVVVSILFFTGTIHAQTFVVFKGDTINRKDAKGLKQGIW
                     KKFYRTDSLFSITSFKDNKQTGTTKFYYESGKLKSTLIWKKPGQLAFAESFFEEGKKM
                     SDGYYLNEQKDSTWHYYNEEGKITSQETYRKGVKQGKWLTYYPEGGKVEEKNYMSGKL
                     EGPYLKFFKDGKLMLKATYKNDKLTGGYTIYNADGGFYTTGQYNAGDRAGKWTVFNEK
                     GKPWIESQYKNGFEVKTDTLQ"
     gene            2626..3732
                     /gene="mnmA"
                     /locus_tag="PKX59_08480"
     CDS             2626..3732
                     /gene="mnmA"
                     /locus_tag="PKX59_08480"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOZ90941.1"
                     /translation="MSKKGRILVAMSGGIDSSVTAMMLHDEGYEVIGITMKTWDYATS
                     GTSGKETGCCSLDSINDARSIAVEKGFPHYILDIRGEFGDFVIDNFVDEYLAGRTPNP
                     CVLCNTHIKWEALIKRADMLDCEYIATGHYASVRNEGGRYIVSRGKDLNKDQSYVLWG
                     LSQKNLARTRFPLGNYKKSEIRNMAFDMGFKELANKSESYEICFVPDNDYRAFLKSRV
                     EGIEEKLDGGVFVNTKNEVIGKHKGYPFYTIGQRKGLEVAFGKPMYVTEIKPNTNTVV
                     LGELEDLQKTTMKVRGLNLVKYESLSNPTEVLTKIRYKDPGTMSVITQTSAVNAAVEF
                     HAPVTAVAPGQSAVFYEGNDVVGGGFIDKEYGKV"
     gene            3719..4390
                     /locus_tag="PKX59_08485"
     CDS             3719..4390
                     /locus_tag="PKX59_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90942.1"
                     /translation="MAKSKNIFSLIILALVLLMGCTKDKETVSAIDYRYYPIAIGDTM
                     IYQVSLFNKDLNTYDSTYQLLERVESVFNDNEGRPTYRLERYVRNTDTDAWSIYKVWT
                     TNKTTTSIEKQEDNITYIKLIFPVKLNKTWNGNAKNIFADDFDDYKIISLNEPLQSGN
                     YSFDSTLNVQQIDYDYGFEKRKHFEIYATGVGMIYKDQYDSLQGSPLGKIYHYTETLL
                     YHNKK"
     gene            4393..6063
                     /locus_tag="PKX59_08490"
     CDS             4393..6063
                     /locus_tag="PKX59_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOZ90943.1"
                     /translation="MKKIYLLILIFLTSHELYSQNRYAVMLTDKNNSSFSFSNPSAFL
                     SQRAINRRLQFGIAIDSLDLPVNATYLTAIQNTGAVILNTSRWLNEVTVDVSVNPNAI
                     AAINALPFVKQTKLASRTTNRTNSKFSFEIESLMERQSQTQKVASTASFYNYGNALNQ
                     IQMLHGDNLHDLGFRGDGKIIAMLDAGFLRADSMTAFDSLRAHNRILSTYDFVDHNSN
                     VYDDHTHGSMCFSIIGANDPGNIVGTAPEASFLLYRSEDAATEKLIEEYNWATAAETA
                     DSAGTDIISSSLGYSVFEDSTMNHTYNDLDGRTAPISIAANIASRKGIVVVSAAGNEG
                     NGQWHYITAPADADSILTIGAVDAAGVYASFSGTGPSFDGRIKPSVVAQGQGTFVSDP
                     YSNTVFSGNGTSFSCPVIAGLVASLWQAIPSANNMQIINAIVQSASQYNNPDSLLGYG
                     IPNFDSARNSLLAVFNPYYGKSDFIEKIFPNPFKNQLNINFYADSNSQYTSEIFDLSG
                     KQVMMKRGKFVPYSINSINLATTAMAKGVYLLRITTEKNVFQSRILKQ"
     gene            6070..7086
                     /locus_tag="PKX59_08495"
     CDS             6070..7086
                     /locus_tag="PKX59_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOZ90944.1"
                     /translation="MSSIGKKLKIIGTNFAFLILFLSLAEVGLRIANLGYDNAAFEPD
                     SVLHHIHRPDYSFIHSNAAEHEYGNIPVRYDKIGCVVNPVKILNVQCNKRVWLFGDSY
                     IEALQVNYDSSVIGILEKDFPDVCFKNFAVGAYSPVLHYLKLKQLLKNDKPDVVLLFL
                     YANDAAEDKQYLNEAKLAGGEVTAVDGGMPQWKYEVIRNSNLLRLIRKFYMQMTVKLE
                     NKKYMNESALSENNAILAEPTLTYLEKFVTLCKKNNVRVIVSCIPSKTDVLRNDFNSA
                     SFSSQIKAWCTGRCDFIDLQEPLQRFYLQTNTSPYYKNDIHFNNYGHRIVAMTIAQLQ
                     TIKN"
     gene            complement(7066..7533)
                     /locus_tag="PKX59_08500"
     CDS             complement(7066..7533)
                     /locus_tag="PKX59_08500"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOZ90945.1"
                     /translation="MSLNINHPAPLFESFNSEKQPFSLEQLKGKNVLLLFFPAAFTST
                     CTKELCSVRDNLSYYNNLNCTVIGISTDTIYSLAKYKEEQKLNFQLVSDFNKEIGKIY
                     HAQYELFGLGMKGITKRAAFLIDKTGILRYIEVLENASEIPDFNKIKSVLDSL"
     gene            complement(7539..8690)
                     /locus_tag="PKX59_08505"
     CDS             complement(7539..8690)
                     /locus_tag="PKX59_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90946.1"
                     /translation="MQYFSLILIISFLFSWFQSTAQHSVLFGERNLNDTILSVYFDRN
                     GKPYPDYFIADSALNINTGSLDTFYLNNPKEFAAICSLYKCRFSQYSISNLSILNDSI
                     FQTNADKINNQTNEGQVPTFMIHGFRKSYVNKPGVFTSVIDYALLMESLNRLSTEKYS
                     YIRVFWDGSYLDISEGVKQMKQIFTVFEKAQFNAISVGYGLRQILSRTTSPEVNIISH
                     SLGAVVVTNAMFDTTVNNYPTPNNSNINICLIAPAISGIEAFKSYYSRKSTYPFKKSD
                     NYSLFVLYNKKDFVLRKKDYFLELFGPGPRKHGNTTLGCNSNNEAIKLKDYFIQNFPN
                     SKITLINMTDLINKCHHLRCYCEGDNLKTVIQLMNHTNINSNGNKILNK"
     gene            8822..9862
                     /locus_tag="PKX59_08510"
     CDS             8822..9862
                     /locus_tag="PKX59_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ90947.1"
                     /translation="MSFQRPGLVALTTGVACGSVFLFSEYLYGISTDNLLFIIVGSIA
                     VAFFVFLLINYFVLASFRSKLINIYRSILDKKNVPYTSVHHAKNIDKVYDDVLNEIKN
                     WESEKQTEIDELKKAENYRREFLGNVSHELKTPLFSAQGYVHTLIDGGIEDSDVNMLY
                     LKKASKSLDRLIAMVEDLESISELETGVVPLEIITFDIVELIKEVNESLEIKANERKI
                     RLIVESETERVYVYADKDKIRQVLVNLIENSIKYGREMGTTKVVVFTHSDSVMIEVAD
                     DGIGISSNNLPRLFERFYRVDKSRSREVGGTGLGLAIVKHIIEAHHQTIKVSSEKDKG
                     TVFSFSLSVSKK"
BASE COUNT         3420 a         1603 c         2014 g         3217 t
ORIGIN      
        1 tttttataca aaaaccaccg gctttcagtc ggtggttttt gtatttttat acctgtatga
       61 aaacatcaga ttcctttaat gccgatgaga tttctttttt tgaaaaagta tatcaggtag
      121 ttcgtttaat tccgaaagga aaagtgacta gttatggtgc catagccaag tatcttggca
      181 gcgcacagtc atcacgtatg gttggttggg cgatgaataa tagtagtcgg ataaaaccaa
      241 aggttcctgc acacagagtc gtaaatcgaa atggcatgct cacaggaaaa cacttttttg
      301 gtgctaatga tgccatgcaa catttgctcg aaaaagaagg cataaaagta tcagacgata
      361 aaataattga ttttaaaaaa cacttttggg atccatctgt tgaattgatg ctataaacac
      421 tcatttacaa ccattatctt tgcaccatgc agattacaaa agaaaaagta ttagaagctt
      481 taagttatgt acaggaacct gatcttaaaa aagatttggt gacactgaac atgattaaag
      541 atattgaaat tgaagggcag aatgttagtt ttaccgtaat ccttacaact ccagcttgtc
      601 ctctgaaagc tgaaattgag cgcgcctgta aaaatgccat agttcatttt gtagataaag
      661 atgctgttgt aacagtaaat atgactgcca atgttactac acagcgcagc atgaataatg
      721 aaatgatgaa aggcgttaaa aatattattg ctgttgcctc cggcaaaggt ggtgtaggta
      781 aatctactgt tgctgctaat ttagcagttt cactagctaa aactggtgct aaagttgccc
      841 ttgttgatgc cgatatattt gggcctagtg tgccaattat gttcggctcg gagcatgagc
      901 atccttttat tgaagaagta aacggaagac aattaatgat accggtagaa aagcatggtg
      961 taaaacttat ttcaattggt taccttgcag atccatcgca ggcaattgtt tggcgaggac
     1021 ctatggcatc aaaagcattg aaacaacttt ttagtgacgt taactggggc gaaatagatt
     1081 atatgattgt tgaccttcct ccgggaacaa gcgatattca tcttacatta atctccgcag
     1141 tacctctgac aggtgcagta gtggtaacaa cgccacaaga tgtagcatta gccgatgcca
     1201 gaaaagcaat agcaatgttt cagcttccat cagtaaatat tcctgttttt ggattaatag
     1261 aaaatatgtc gtggttcact cctgaagagc ttcctgacaa taaatattat ctttttggaa
     1321 aggatggtgg acaaaaatta gctgcagaaa tgaatgtaaa attactcggt caggttccat
     1381 tagttcagtc tgttcgcgaa ggtggcgata taggaaatcc aattgcaaca ggaaattcaa
     1441 tagtttcaga agcatttaac gaattatcaa aatctgttgc acaacaggta gcaattcgta
     1501 atgctgttat tgagccggca cagcgtgttc ctgctgcagg attttaaaaa gtgtaagaat
     1561 gagtgagtca ttaaaaaata atttaaaaca taaggtcgag caggcattgg ataccattcg
     1621 gccctatttg aaatccgata atggtgatgt taccgttctt gatattacac cacaaaatgt
     1681 tgttaagttg aggtttgaag gagcatgcag ttcgtgtaca atgagtgcaa tgacatttaa
     1741 agcaggagtt gaagaaacta ttttacggat ggtgcctgaa attactgctg tagaagcatt
     1801 gaatgtggaa aatgtttaaa tggtaatttt atctaaaata aaacagactt gttgatgtta
     1861 tctcttaata agcgttttct tccaatgaaa attacagctg ctgttgttgt ttctatttta
     1921 ttttttaccg gcactataca tgcacaaact tttgttgttt tcaaaggcga tactataaat
     1981 cgtaaagacg ctaaaggact taaacaaggt atatggaaaa agttttacag aacagacagc
     2041 ttgttttcga taacatcatt taaagacaac aaacaaactg gtacaacaaa attttattac
     2101 gaatcaggta aattaaagtc aacactcatt tggaaaaaac ccggtcagct tgcttttgca
     2161 gaaagctttt tcgaagaagg aaaaaaaatg tctgatggtt attatctgaa tgaacagaag
     2221 gacagcactt ggcattatta taacgaagag ggtaaaatta catcacagga aacctatcgc
     2281 aaaggtgtaa aacaagggaa atggcttacc tattatcctg aaggtggaaa agtagaggag
     2341 aaaaattata tgagtggtaa attggaaggg ccatacttaa aattttttaa agatggaaaa
     2401 ttaatgctaa aggcaaccta taaaaacgat aaacttacag gtggttatac catctataat
     2461 gcagatggtg gattttatac aacagggcaa tataatgcgg gagacagggc aggtaagtgg
     2521 actgttttta atgaaaaagg caagccgtgg attgagagtc aatataaaaa tggatttgaa
     2581 gttaagacag atacacttca gtaataatta aagtaacttt gcaaggtgag taagaaaggt
     2641 agaattttag ttgccatgag tggtggtatt gatagctctg ttacagctat gatgctgcat
     2701 gatgaaggtt atgaagttat aggcatcaca atgaaaacat gggattatgc aacatcggga
     2761 acgtccggta aagagacagg atgttgcagc ctcgactcaa taaacgatgc cagaagtatt
     2821 gctgtagaaa aaggatttcc gcattatata ctcgatatca gaggtgagtt tggcgatttt
     2881 gtgattgata attttgttga tgaatacctt gccggacgaa cacccaatcc ctgtgtgctt
     2941 tgtaatacac atatcaaatg ggaagcatta attaaacgtg ccgatatgtt agattgtgag
     3001 tatatcgcta ccggacatta tgcatctgta cgcaatgaag gaggtcgtta cattgtcagc
     3061 agaggaaaag atttaaataa agatcaaagc tatgtgttgt ggggtttaag tcagaaaaat
     3121 ttggcgcgaa cacgatttcc attagggaat tataaaaaat cagaaattcg caatatggca
     3181 ttcgacatgg gatttaaaga attagccaat aaaagtgaga gctatgagat ttgttttgtt
     3241 ccggataatg actacagagc atttttaaaa tcaagagttg aaggtattga agaaaaactt
     3301 gatggtggcg tatttgtgaa tacaaaaaat gaagtaatag gaaagcataa aggatatcct
     3361 ttctatacaa ttggtcagcg taaaggtctt gaagttgcat ttggtaagcc aatgtatgtt
     3421 acggaaataa aaccaaacac aaatactgtg gtattgggtg agctggaaga tttacagaaa
     3481 actactatga aggtacgtgg attaaatctt gtaaagtatg aatcgctcag taatcccaca
     3541 gaagtactta caaaaatacg gtataaagac ccgggcacta tgagtgtcat aacgcaaaca
     3601 tctgcagtca atgctgctgt tgaatttcat gcaccggtta ctgctgttgc tcccggacag
     3661 agtgctgttt tctacgaagg caacgatgta gtaggaggag gatttattga taaagagtat
     3721 ggcaaagtct aaaaacattt tctcactgat catcctggct ttagtattat tgatggggtg
     3781 tactaaagac aaggaaacag ttagtgccat tgactatcgt tattatccaa tagcaattgg
     3841 cgatactatg atctatcagg ttagtttgtt taataaagac cttaacacct atgatagtac
     3901 ctatcagtta ttagaaagag tagaatctgt ttttaatgat aacgaaggtc ggccaacata
     3961 ccgtttagaa cgttatgttc gtaataccga tacagatgca tggagtattt ataaggtgtg
     4021 gacaaccaat aagactacta ccagtataga gaaacaagaa gacaacatta cttatataaa
     4081 actcattttc cctgtaaaac tcaataaaac atggaatggt aatgcaaaaa atatttttgc
     4141 tgatgacttt gatgattata aaattatttc tctgaatgaa ccattgcaat caggaaatta
     4201 ttcatttgac agtactttaa atgtgcaaca aatagattat gattatggct ttgaaaaaag
     4261 aaagcatttt gaaatttatg ccaccggagt aggaatgatt tacaaagacc agtatgattc
     4321 tttgcaagga agtccgcttg gtaaaattta tcattacaca gagacattac tttatcacaa
     4381 taaaaaataa gcatgaaaaa aatttacttg ttgattctga tttttctgac atcacatgag
     4441 ttgtatagtc agaatcgtta tgcagtaatg ctgacagata aaaataactc ttcttttagt
     4501 ttctctaatc catctgcctt tttatctcaa agagcaatta acagaagatt gcaatttggt
     4561 attgctatag attcactcga tttaccggtt aatgcaactt atcttacagc aattcaaaat
     4621 accggagcag taattttaaa tacatcacgc tggctaaatg aagtgacagt agatgtatcc
     4681 gtaaatccaa atgcaatagc agcaataaat gcattgcctt ttgtgaaaca gacaaaattg
     4741 gcttcacgca caactaacag aacaaacagc aagttcagtt ttgagataga aagtttaatg
     4801 gagcgtcaat ctcagacaca gaaagtagct tcgactgcat cattttataa ttatggaaat
     4861 gcactcaatc aaattcaaat gctgcatggc gacaacttac atgatttagg attcagaggt
     4921 gatggaaaaa ttattgctat gcttgatgcc ggatttctta gagctgacag tatgactgca
     4981 ttcgacagtc tgcgtgcaca taacagaatt ttgtccactt atgattttgt tgatcataac
     5041 agtaatgttt atgacgacca cacacatggt tccatgtgtt tttcaataat tggcgctaat
     5101 gatcccggaa atattgttgg tactgcacct gaagcttcat ttctattgta tcgttcagag
     5161 gatgcagcaa cagaaaagct gatagaagaa tataactggg caacagcagc agaaacagca
     5221 gacagtgcag gaacagatat tatctcaagt tcattaggat atagtgtgtt tgaggattct
     5281 acaatgaatc atacttacaa tgatttggat ggcagaacag cacccatatc cattgcagcc
     5341 aacattgctt cacgaaaagg aattgttgtt gttagcgctg ccggtaatga aggtaatggt
     5401 caatggcatt acataacagc acctgcagat gccgatagta ttttaacaat tggcgctgtt
     5461 gatgcagcag gagtatatgc ttcattcagt ggcacaggtc catcttttga tggaagaata
     5521 aaaccatctg tagttgcaca aggacaaggc acatttgttt cggaccctta ttcaaatact
     5581 gttttctccg gtaatggaac ttctttttca tgcccggtaa ttgcaggatt agtggcttca
     5641 ttgtggcaag caattccatc tgcaaacaac atgcaaatta ttaatgccat agtgcagagt
     5701 gcatcacagt ataacaatcc tgactcattg ctgggttatg gcattcctaa ttttgattct
     5761 gcacgtaact ctttacttgc agtttttaat ccatattatg gtaaaagcga ttttatcgaa
     5821 aaaatatttc ctaatccatt taagaaccaa ctgaacataa atttttatgc agatagtaac
     5881 agtcaatata cttcagaaat atttgatctc agtggaaagc aagtaatgat gaaacgtggc
     5941 aaatttgttc catattctat caacagcata aaccttgcta ccacagccat ggcaaaagga
     6001 gtttatttgt tgcgtatcac tactgaaaag aatgtttttc agtcgcgaat tttgaaacaa
     6061 taggcttcaa tgtcatcaat agggaagaag cttaaaatta taggaaccaa cttcgctttt
     6121 ttaatattgt ttttatccct tgcggaggtt ggattacgaa ttgcaaatct cggttatgac
     6181 aatgcagcct tcgaacccga ttctgttctg catcatattc acagacctga ttattcgttt
     6241 attcattcaa acgctgcaga gcatgagtac ggtaatattc ctgtcaggta tgataagata
     6301 ggttgtgttg ttaaccctgt taaaattctg aatgtgcagt gtaataaaag agtttggtta
     6361 tttggcgact cttatattga agcattgcaa gtaaattatg attcatctgt tataggtatt
     6421 ttagaaaaag attttccgga tgtatgtttt aaaaattttg ctgttggtgc atacagtcca
     6481 gttctgcatt atttaaaatt aaaacaattg ttgaagaatg ataaacctga tgttgtcctt
     6541 ttgtttttat atgccaatga tgctgcagaa gataaacagt atcttaatga agccaagtta
     6601 gctggaggag aagtaacggc tgttgatggt ggtatgccac aatggaaata tgaagttatt
     6661 aggaactcaa acctgcttcg tttgatacgt aaattttata tgcagatgac tgtgaaactg
     6721 gaaaataaaa agtacatgaa tgagtctgct ttgagtgaga ataatgcaat tcttgcagag
     6781 ccaacactaa cttatttaga aaaatttgtt acactttgta agaaaaataa tgttagagtt
     6841 atagtgtcgt gcataccttc aaagacagat gttctgagaa atgattttaa ttctgctagt
     6901 ttttcaagtc agataaaagc atggtgtacc ggaagatgtg attttataga tttacaagag
     6961 cccttacaga gattctattt gcaaactaac acttcaccat attacaaaaa tgatattcat
     7021 tttaataact acggccaccg cattgtggca atgacaatag cgcaattaca aactatcaag
     7081 aactgatttg attttattaa agtcaggaat ttcacttgca ttttctaata cttctatgta
     7141 acgtagaatc ccggttttat caatgagaaa tgcagcacgt tttgtaatac ctttcatccc
     7201 taatccaaac aactcatatt gtgcatgata aattttacct atttctttgt tgaaatcgga
     7261 gactaactga aaattcaact tctgctcttc tttatatttt gctaacgaat aaatagtatc
     7321 ggttgagatg ccaattactg tgcagtttaa gttattataa taagacagat tgtctctgac
     7381 actacacaat tcttttgtac aagtactggt aaatgctgca ggaaaaaata ggagtaaaac
     7441 attcttgcct tttaattgct caagtgaaaa cggttgcttt tctgaattga aactttcaaa
     7501 aagtggtgca gggtgattaa tatttagtga cataatattt atttgtttaa tattttattt
     7561 ccgtttgaat taatatttgt atggttcatc aattgtataa ctgtttttaa gttatcgcct
     7621 tcgcaatagc aacgtaaatg gtgacattta ttaattaagt ctgtcatatt tatgagagta
     7681 atttttgaat tagggaaatt ttgaataaaa taatctttta gtttaattgc ttcattgttt
     7741 gaattacatc ccaaagtggt gttaccatgc tttcttggcc ccggaccaaa caactctaag
     7801 aaatagtctt tcttgcgcaa cacaaaatct tttttattgt ataatacaaa tagactatag
     7861 ttgtcactct ttttaaatgg gtatgtgctt tttcttgaat agtaactttt aaatgcttct
     7921 attccgctta ttgcaggtgc aattagacat atattaatat tgctgttatt gggagtagga
     7981 tagttgttta ctgttgtatc aaacatagca ttggtcacta ctacagcacc aagactgtgt
     8041 gatataatgt tgacttccgg tgaagtggtt cgtgaaagta tttgccttaa tccatagcca
     8101 acagaaatag cattaaattg cgctttctca aagactgtaa aaatttgttt catctgcttt
     8161 acgccttccg atatatccag ataactacca tcccaaaaca cccttatata ggaatacttt
     8221 tcagtgctta gacgatttaa cgactccatt aataaagcat agtctatcac agaagtaaaa
     8281 acgccaggtt tgttaacata agattttctg aaaccatgaa tcatgaaggt cgggacctga
     8341 ccttcatttg tttgattatt tattttatca gcattggttt gaaaaattga atcatttaaa
     8401 atgcttaaat ttgaaatact atactgacta aaacgacatt tatataaaga acatattgcg
     8461 gcaaattctt tcggattatt taagtaaaaa gtgtctaatg atccggtatt aatattcagt
     8521 gcagagtcag caataaaata gtccgggtat ggctttccgt ttctgtcaaa atagactgat
     8581 agaattgtgt cattcaggtt tctttctcca aacaagacag aatgctgtgc cgtactttga
     8641 aaccaggaaa acagaaatga aattattagt atcagtgaaa agtactgcat aaatattaga
     8701 acagtttaaa aacgcattta tccattgatt acaagaatga tatttaaaaa ggcaaaaata
     8761 tcttttaaat ttttaccaat attgttctga gtgatttgtt gaagttggct aattttgtga
     8821 aatgtcattt caaagaccgg ggttagttgc attaacaaca ggagttgcct gtggttctgt
     8881 ttttttattt agtgaatatt tatatggaat tagtactgat aatttacttt ttataattgt
     8941 tggtagtatt gccgttgctt tcttcgtttt tctcctcata aactattttg ttttagcatc
     9001 attcaggagc aaactaataa acatttacag aagcattctc gataagaaaa acgtgccata
     9061 tacctcagtg catcatgcta agaatattga caaggtttat gatgatgtgc ttaatgagat
     9121 caagaattgg gagagtgaga agcaaacaga aattgatgaa ttaaagaaag cagaaaacta
     9181 cagacgtgaa tttcttggta atgtatcaca tgaacttaag actccactct tcagtgctca
     9241 ggggtatgtt catactttga ttgatggagg cattgaagat tcagatgtaa atatgcttta
     9301 tctgaaaaaa gcatccaaga gcctcgacag actaattgcc atggttgagg atctcgagtc
     9361 aatatcagaa ttggagacag gtgttgtccc tctagaaatt ataacttttg atattgttga
     9421 actgattaag gaggttaatg agtcattaga aattaaagca aacgaacgta aaatcaggtt
     9481 gattgttgag tcggaaacag aacgtgttta tgtctatgcc gataaagaca aaattcgtca
     9541 ggtgcttgtg aatctgatag aaaactcgat taaatatggt agagaaatgg gtactacaaa
     9601 agttgttgta tttacacaca gtgattcggt gatgatagaa gttgcagatg atggtattgg
     9661 tatttcttcc aataatcttc cacgattatt cgagcgattt tacagagttg acaaaagcag
     9721 gtcacgagaa gtaggaggga ctgggcttgg tttagctatt gtgaaacata ttattgaggc
     9781 acatcatcaa actattaaag ttagtagtga aaaagataaa ggcaccgttt tttcattcag
     9841 cctgtcggta agtaagaaat aagcctttta ttgttgttgt tcgaagtcat tcctttgtag
     9901 aagacattgt gagattgtga atagataacg taaacagtga acataaataa aaaagtccgc
     9961 atttgcggac tttgtgggag caacaggatt cgtcagccgc ggctgactgt accctctgct
    10021 agagaatgat cttggatttg agaaagtctc taccggcacc ggttttaaaa tatttttctc
    10081 ttttcagcgc atccgattta gaaagaaaag tttcagaata aaccaacctc caaggagctt
    10141 tatttttggt gtaatttcca agtagtccat tgttgtgtgt gaatattata cacatcagct
    10201 ctacagccag acctatctac aagacatatt cggtattcga atataaaaaa taaa
//
LOCUS       DAOOOI010000071        10253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1276_length_10253_cov_13.656305, whole genome shotgun
            sequence.
ACCESSION   DAOOOI010000071 DAOOOI010000000
VERSION     DAOOOI010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10253
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1276_length_10253_cov_13.656305"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PKX59_08515"
     CDS             <1..472
                     /locus_tag="PKX59_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOZ90948.1"
                     /translation="ILRIIRELINFEAESKGTSISAGLQFLSNAIKKKSIAFLISDFY
                     DSAVGKPKSMLEDSLKIANRKHDLVVLKINDLRESELPDIGLINIYDAETGREQLIDT
                     SSAVVRKNYQNQRQMRNRQINGLLSKSGIDKIELFTGQNYIQPLMKLFKNRVKK"
     gene            469..1434
                     /locus_tag="PKX59_08520"
     CDS             469..1434
                     /locus_tag="PKX59_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90949.1"
                     /translation="MKLFIKKIPALLISILLSANCVFAQSVTAVLQLDTNRIVAGQQI
                     TAKLRFTAPADYQTKWVAIPDTFNGIDVISRLPIDTLPSANKSLITREQKFTLTAFDS
                     GFFVITPFVFSYKKTGDTTNYTVETQAQLLTSNLMPVDTSKAIHDIKGPMDVGITWQE
                     VAMYSSIILLLIAIIWYVVYRLRKKKPQTVIKVPDAPKRPAHEIAIEQLEHLQQEKLW
                     QQGNFKMYYTQLSDIMRAYISNRWNFDAMESTTDEIMHSNTALQLSNDNFNRLKSTLT
                     LSDLAKFAKYTPLSNENEQAMTDAFIFVNETKVVLTTKPQEQVKP"
     gene            1456..2439
                     /locus_tag="PKX59_08525"
     CDS             1456..2439
                     /locus_tag="PKX59_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOZ90950.1"
                     /translation="MQFAYPQFFWLLLLIPAMLLWQWKVTSAKKVRLKLSSDDGFQNY
                     QPSFRQRLVFMPFVFRLLSVICIIIALARPQSSSQAQQVSTEGISIVLAMDISGSMLA
                     EDFKPNRIEAAKKVAIDFIDHRPNDLIGLVIFSGESFTQCPLTSDHAVLKNLMSKIES
                     GMLTDGTAIGEGLATAVNRLKDAKTKSKVIILLTDGVNNAGAIAPLTAGEIAKTFGIR
                     VYTIGVGTVGMAPYPFKTVFGIQYQNVPVQIDEEICKQISDATDGKYFRATNNKTLKE
                     IYSEIDKLEKTKVEVTEFRRHSEEYFNYALAAGIFFALELLLKYTWLRKLT"
     gene            2463..3515
                     /locus_tag="PKX59_08530"
     CDS             2463..3515
                     /locus_tag="PKX59_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010423183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOZ90951.1"
                     /translation="MFRFSHSFILYCLALIPLFIILYISLVNWRKKSLKKFGDMHLVR
                     FLTPGKAYTKPLIKFILLMTAFLFLVIGIAGPQVGTKLETVKRKGVDVIIALDVSNSM
                     NAEDIKPSRLERAKQSISRLIDQMQGDRIGLIVFAGEAYTQLPITTDYGAAKLFLSTV
                     NTNIVPTQGTAIGKAIDLAVSSFGNEDSSKKNKAIIVITDGENHEDDAVAAAKEAYKN
                     NITVHTIGMGSVNGAPIPVYTNGQRTGFMQDNNGQTVITKIDEQTLAEIAQAGNGKFI
                     RATNSDDGLSIIMKEINAMQKNEFGSKMFTDYADQFQYLLGAALFFLILEFLISEKRS
                     RWMEELNLFGDGDEKK"
     gene            3541..4224
                     /locus_tag="PKX59_08535"
     CDS             3541..4224
                     /locus_tag="PKX59_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ90952.1"
                     /translation="MSFVAIAQNEKPLVREGNKEYKNGKFDQAESSYRKAIEKNGNSV
                     EGLYNLGNALYKQNKFDEAAGVYQNLNNEKLSTDAKAKSLYNLGNTLMKSEKYQEGAN
                     AYKEALKLSPNDKDALYNYSYALAKLKQQQQQQKQNQDNKKDQKNQQKKQQDKQQQKQ
                     DQQKQQQDQQKEQDQQKQDQKKEQAQQPKISKEDAERMLEALKNDEMNLQKKLAKKEG
                     TRVNIEKQW"
     gene            4218..6083
                     /locus_tag="PKX59_08540"
     CDS             4218..6083
                     /locus_tag="PKX59_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOZ90953.1"
                     /translation="MVETKAIKIKHKSLFAGLVLLLLFFTTNIFAAEITFIASVNKNP
                     VGTNEQFSITYTLNTQGSNFQAPTFRDFNVLSGPNQSTSMQFINGNMSQSVSFTYYLT
                     ANSEGTFRIDPATITVNGGKVKSNTLTINVVKSQKAQSGAQQQKSNDADAGISSNSVF
                     LRVSVNKSSVYRGEALLATYKLYTKVNLVNYSIDKLPALNGFWSQELKMPQQLELTTE
                     NYNGEMYRVGIVKKVLLFPQQSGTLTIEPMEGSCIARIQTQRKRSNNPFDIFNDPFFN
                     DPFFGSAQDVKVNVKSLPVKITVKDLPQENDNAFKGAIGSFSLQATTDNDKVKANDAI
                     NLKIKISGKGNLKLIDAPEVEVPTDFEKYDPKVSDNTNISESGVSGSKTFEYLLIPRH
                     EGEYTIDPIKFTYFDLDKKQYEQLQSSAFKITVKGISGDASAAQSVATSQNKTDIKLL
                     GKDISFIKTGKLTTKPATTFYLSGLYYLLVAIPFAALILLILYIRKRNRLAGDAEYMR
                     SSRATKMAHKRLSIAKKMIDAKKDDAFYEEISKAIYGYASDKLNINFSEMTKENLRQK
                     LINRNIPDALTERIIATIDLCELARYAPLTQNNNTQSIYTEAAAIITELENYLNK"
     gene            6087..6848
                     /locus_tag="PKX59_08545"
     CDS             6087..6848
                     /locus_tag="PKX59_08545"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ90954.1"
                     /translation="MKARYIFILLCLLLSQNIFADSKHDIVFNEANNLYLKQSYFQAI
                     EKYEQLVADNTISFEVYYNLGNAYYKTGNFTKAILNYERAKKIKPDDADVNLNLAIAN
                     QKTIDKIEPTPKVFYNQWWDNYIASSTADSSAVIMLFFLWLFIAAIAIMIIIKSASLK
                     KLCFIISLILACCTLFFFITTQTRQNINDSSVEALIISQSAYIKSAPDDKGTNLFQLH
                     EGTKINIVDELSSWKKIRIPNGNIGWIKSKDLETI"
     gene            complement(6849..8744)
                     /gene="asnB"
                     /locus_tag="PKX59_08550"
     CDS             complement(6849..8744)
                     /gene="asnB"
                     /locus_tag="PKX59_08550"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOZ90955.1"
                     /translation="MCGIAGSFSVSNFFSEQDFRKASAAIKHRGPDGDGFYSDNFVRL
                     AHRRLSIIDLDNRASQPMYSSCMRYVMVYNGEVYNYVELRKEIESEYKDSQLSDFKTT
                     SDTEVILRLFEIKGVDFVKDLNGMFAIAIYDTQANKLFLFRDRFGIKPLYYYHDSSGN
                     FCFASELKAILAIDSIERKVNSQAVAYFLHTGFVPAPLTMYEKVFKLESGNFLTIDRN
                     GLEIHPFVMNRKCSQREIEHDEKKAIQNIEKLLIQSVKIQLRSDVPYGVFLSGGVDSS
                     LLTAIAARNVDEKLNTFSIGFAEQSHNESEHARVIAKHIGTNHHEFVVSVKDAIDLIE
                     TFFEVYDEPYTDTSGIPTMLVSKLASKHVKVVLSGEGGDELFFGYGTHNWAKRLNAFP
                     FSVFRSPIRTILTCGNSRSKRVAQLLDFNEHSDFLPEHIYSQEQYLFSNKEIVELVSD
                     ELRKETLKNIAQRNEELEKLFNINDSCLPEERQANYELKFPFQDDLLTKVDRATMFHS
                     IEARVPFLDNNFAEYVFSVSSDIKIKNGINKYPLKKILANYVPEELFMRPKKGFSIPL
                     AKWLKADWKYLLNRYLDEKTIRHAGLVKFEVVKKLIQRFLSGEDYLYNRIWCLIVLHR
                     WYERQQW"
     gene            complement(8744..9637)
                     /locus_tag="PKX59_08555"
     CDS             complement(8744..9637)
                     /locus_tag="PKX59_08555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ90956.1"
                     /translation="MLTDKPPFFSVIMPVYNRADLVKDSIVSVLNQSFKDFELICIDD
                     GSTDNTLQVLKEVSKIDRRIKLISHQNKGRCAARNNGINAASGDWIAFLDSDDYYLEN
                     HLSTMYKLVQEFPSFSGIATELVVESKIRDYANCNYYRDQRVFSLSDFAEANPISLNQ
                     FCYKKSHYPELLFPDINILTAEDLLFFRMFSFRYKILKSGIITNFVNSHVNRSVNLIS
                     TEQFVYWNKFATNYFIDNFSLPTNIKAKMQQYLQLFVSNVYLSSNYTRKGLTELMKVL
                     KYPSTYTDMHFYKAIAKIFIS"
     gene            complement(9627..>10253)
                     /locus_tag="PKX59_08560"
     CDS             complement(9627..>10253)
                     /locus_tag="PKX59_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90957.1"
                     /translation="VGILRTVEAGWICLLKPGDEWLPYHLSAFRDAMLHFKDFQAYLT
                     LNYLRGYSTIKSVGPEDNIINVKKIELKEQLSLSQLMIHSSLLKSNVFPDFKVDSHFD
                     TLFELILVCSSGMVRINRFTNICTLRYPIHTKAYFDQQTLIVCYLIRNIVKDHRLRFY
                     LLFINWQIGFMGLLKAFRLIGAGVFFKEMLCYYVGLKRGVKKGQIYVD"
BASE COUNT         3708 a         1822 c         1704 g         3019 t
ORIGIN      
        1 cattttgcgc atcatacgtg agttaataaa ctttgaagct gagagtaaag gcacatcaat
       61 tagtgctgga cttcagtttt taagtaatgc tataaagaaa aagagtattg cgtttttaat
      121 ttccgacttt tacgatagtg ctgtcggcaa gcctaaaagt atgcttgaag attctttaaa
      181 aattgccaat cgtaaacatg atttggttgt cttaaaaata aacgacttgc gcgaatcaga
      241 gttgcctgac attggattaa ttaatattta tgatgcagaa accggtcgcg aacaacttat
      301 tgatacttca agtgctgttg tcagaaagaa ttatcagaac cagcgacaaa tgcgcaatcg
      361 ccagatcaat ggattgttaa gcaaaagcgg cattgataaa atagaattat ttacagggca
      421 gaactatatt cagccattga tgaaactttt taagaacaga gttaaaaaat gaaattattt
      481 attaaaaaaa ttcctgctct gcttataagt attttacttt ccgctaattg tgtatttgca
      541 cagagtgtaa ctgctgttct tcaacttgac accaacagaa ttgttgcagg gcagcaaata
      601 acagctaagc tccgttttac tgcacctgca gactatcaaa caaaatgggt tgccattccg
      661 gatactttta atggcattga tgttatttcg cgcttgccta ttgacacctt accctccgcc
      721 aacaaaagtc taattacacg tgagcaaaaa tttacactta ctgcttttga ttcaggattt
      781 tttgtaataa ctccatttgt attcagctat aaaaaaacag gcgataccac aaactatact
      841 gtagaaacac aagcacaatt actgacttca aatctgatgc ctgttgacac ctctaaagcc
      901 attcatgata ttaaaggacc aatggatgtt ggaataacct ggcaagaagt tgcaatgtat
      961 tcttcaataa tacttttgct aattgcaata atatggtatg ttgtctatcg cctgagaaag
     1021 aaaaaaccac agacagttat aaaagttcct gatgctccaa agcgaccagc tcatgaaatt
     1081 gcaatagaac aattagagca tcttcaacaa gaaaaattat ggcaacaagg taatttcaaa
     1141 atgtattaca cacaactttc agatataatg agagcataca tcagcaaccg ttggaatttt
     1201 gatgccatgg aaagtaccac tgatgaaata atgcatagca acactgcctt gcaattaagc
     1261 aatgacaatt ttaacagact gaaaagcaca ttaacgttaa gtgatttggc aaaatttgca
     1321 aaatacacac cgttgagtaa tgaaaatgaa caagcaatga cagatgcttt catttttgta
     1381 aacgaaacca aggttgttct gaccacaaaa ccacaagaac aggttaagcc ataaaacatg
     1441 agtttatttg ataacataca atttgcctat cctcagttct tctggttgct gttgctgatt
     1501 ccggcaatgt tgttgtggca gtggaaagtt acttctgcaa aaaaagtcag gcttaaactt
     1561 tcatctgatg atggatttca aaattatcaa ccaagtttca gacagcgatt ggtgttcatg
     1621 ccatttgtat tccgacttct cagcgttatc tgcattatca tagctcttgc acgtccacaa
     1681 tcatcatcac aagcacagca ggtttcaaca gaaggcatat caattgtttt ggcaatggac
     1741 atatcgggaa gtatgcttgc agaagatttt aaaccaaaca gaattgaagc tgctaaaaaa
     1801 gtggctattg attttattga tcatcgtccg aatgatttaa ttggtttggt aattttcagt
     1861 ggggaaagtt ttactcaatg tccattaact tcagaccatg cagtattaaa aaacttgatg
     1921 tcgaaaattg aaagtggtat gctgacagat ggcactgcta ttggagaagg cttggcaaca
     1981 gctgtgaacc gtctgaaaga tgctaagaca aaaagtaagg ttattatttt acttactgac
     2041 ggtgtaaata atgcaggagc cattgcacca ttaacagcag gtgaaatagc taaaactttc
     2101 ggcatcagag tctataccat tggcgttggc acagttggca tggcacccta tccgtttaaa
     2161 actgtttttg gaatacaata tcagaatgtt cctgtgcaga ttgatgagga aatctgcaag
     2221 caaatttctg atgcaacaga tggaaaatat ttccgtgcaa ctaacaataa aactttaaaa
     2281 gaaatctatt ctgaaattga taaactcgaa aaaacaaaag ttgaagtaac tgagttcagg
     2341 cgtcatagtg aagagtattt caattatgct ctggcagcag gaatattttt tgcactagaa
     2401 ctactattga aatatacctg gttaagaaaa cttacataaa agataaagat taaagcgtaa
     2461 caatgttccg attttcacat agttttattt tatattgtct ggctctgata ccgctattta
     2521 tcattctata tataagtttg gttaactggc gaaaaaaatc actgaaaaaa tttggagata
     2581 tgcatttggt cagatttctc actccgggaa aagcatatac caagccactt ataaagttta
     2641 ttttactgat gactgcgttt ctatttctgg taattggaat tgctggtcct caggtaggca
     2701 ctaaattaga aactgttaaa cgcaaaggtg ttgatgtcat cattgcgtta gatgtgagta
     2761 acagcatgaa tgccgaggac attaaaccaa gccgactgga gcgagcgaaa caatcaattt
     2821 caaggttaat tgatcagatg caaggcgaca gaatcggctt aatagttttt gcaggtgaag
     2881 cttatacaca actacctata actacagact acggtgctgc aaaattgttt ctttcaacag
     2941 taaacaccaa cattgttcca acgcagggaa ctgccattgg aaaagctatt gacttggctg
     3001 tatcatcttt cggcaatgaa gattcttcga aaaaaaacaa agccatcatt gtgattactg
     3061 atggagaaaa tcatgaggat gatgcagtgg ccgcagctaa agaagcatat aaaaacaaca
     3121 ttactgttca taccattggc atgggttctg ttaatggtgc tcctattccg gtttatacaa
     3181 atgggcaacg cacaggattt atgcaggaca ataatggtca gactgtaatc actaaaatag
     3241 atgagcaaac cttagcagaa attgcacagg ccggaaatgg caaatttatc agggcaacaa
     3301 atagtgatga tgggcttagc attataatga aagaaatcaa tgctatgcaa aaaaacgaat
     3361 ttggcagtaa gatgtttaca gactatgcag accaatttca atatcttttg ggtgctgcat
     3421 tgttttttct tatcttagag tttctgattt cagaaaaaag aagcagatgg atggaagaat
     3481 taaatttatt tggcgatggt gatgaaaaaa agtaatattc ttatcttatt ttttatttca
     3541 atgagttttg tagctattgc acaaaatgaa aaaccattgg tacgcgaggg caataaggaa
     3601 tataagaacg gaaaatttga tcaggcagaa tcaagctatc gtaaagccat cgaaaaaaat
     3661 ggaaattctg tagaaggtct ttataattta ggcaatgcac tgtacaaaca aaacaaattt
     3721 gacgaagctg caggagttta tcaaaacctg aacaatgaaa aactttctac tgatgccaaa
     3781 gctaaatctc tttacaacct cggtaacaca ttgatgaaat ccgaaaaata tcaggaaggt
     3841 gcgaatgcct ataaagaggc tttaaagctt agtccaaatg ataaagatgc tttatataat
     3901 tattcttatg cattagctaa attaaaacaa caacaacagc agcaaaaaca aaatcaggat
     3961 aataaaaaag atcaaaagaa tcaacaaaaa aagcaacagg ataagcaaca acaaaaacaa
     4021 gatcaacaaa agcagcaaca agaccaacaa aaagaacagg atcaacaaaa acaggatcag
     4081 aaaaaagagc aagcacagca accaaaaatt tcgaaagaag atgcagaacg tatgcttgaa
     4141 gcactgaaaa atgatgaaat gaatttgcag aagaaattag ctaagaaaga aggcacaaga
     4201 gttaatattg aaaaacaatg gtagaaacaa aagctataaa gataaaacac aagtcgttat
     4261 tcgcaggctt agtgcttcta ttgttgtttt ttacaactaa tatttttgct gcagaaataa
     4321 ctttcatagc atctgtaaac aaaaaccctg ttggcaccaa tgagcaattc agtataacct
     4381 atactttaaa cacacaaggc agtaatttcc aagccccaac ttttagagat ttcaatgtgt
     4441 tgagcggccc taatcaaagc acgagcatgc aatttataaa cggaaatatg tcgcagtccg
     4501 tttcattcac ctattatctt acagccaaca gtgaagggac ttttcgaatt gaccctgcaa
     4561 caataaccgt taacggagga aaagtaaaat ctaataccct tacaataaat gttgttaaaa
     4621 gtcaaaaagc acaatcgggt gctcaacaac aaaaaagcaa tgatgctgat gcaggcatta
     4681 gcagcaatag tgtattttta agagtgagtg taaacaaatc atcggtttac agaggagaag
     4741 cactgttagc gacatataaa ttatatacta aagtaaattt ggttaactat agtattgata
     4801 aactacctgc actaaatggg ttctggtcac aagaattaaa aatgccacag caattagaac
     4861 tcactacaga aaattacaat ggtgagatgt atagagttgg aattgttaaa aaagtattgt
     4921 tgtttccaca gcaatcaggc acattaacta tagagccaat ggaaggcagt tgcattgccc
     4981 gtattcaaac acagcgcaaa cgttccaata acccattcga catttttaac gacccatttt
     5041 ttaacgaccc gtttttcgga tcggcacaag atgtaaaagt taatgttaaa agcttacctg
     5101 taaaaattac agtaaaagat cttcctcagg aaaatgacaa tgcatttaaa ggggcgattg
     5161 gatcatttag tttgcaggca acgactgata atgataaagt taaagctaat gatgctatta
     5221 atttaaaaat aaaaatatcc ggcaaaggaa atttaaaact tattgatgca cctgaagttg
     5281 aagtgccaac tgattttgaa aagtatgatc caaaagtcag cgataataca aatatttcag
     5341 aaagtggtgt ttcgggcagc aaaacttttg aatatttatt gataccacgt catgaaggtg
     5401 aatatacaat tgacccaata aaattcacct atttcgatct tgacaagaaa cagtatgaac
     5461 agttacaatc atcagcattt aaaattacag ttaaaggaat ttcgggagat gcaagtgctg
     5521 cacaatcagt agcgacatct caaaacaaga cagacattaa gcttcttggc aaagacattt
     5581 cattcattaa aaccggaaaa ttaacaacaa aaccggcaac aacattttat ctgagtggtt
     5641 tatactattt attggttgca atcccatttg cagcacttat tttattgata ttgtatatca
     5701 gaaaaagaaa tagacttgcc ggtgatgcag aatacatgcg aagtagcaga gctacaaaaa
     5761 tggcacataa acgattgtca attgctaaaa agatgattga tgcaaagaaa gatgacgctt
     5821 tctacgaaga aatatccaaa gctatatatg gatatgcttc tgacaaacta aatattaatt
     5881 tttctgagat gacaaaagaa aatctaaggc aaaaattaat caacagaaat attcctgatg
     5941 ctttaactga aagaatcatt gcaacaattg acttgtgcga acttgccaga tacgctccac
     6001 tcacacaaaa caataacaca caaagcattt acactgaagc tgctgccatc attacagaac
     6061 tcgaaaacta tttaaacaaa taaaatatga aagcaagata tatattcatc ttactatgcc
     6121 tgttgctttc gcagaatatt tttgccgact caaaacatga tatagtcttt aatgaagcta
     6181 acaaccttta tttaaaacaa agttattttc aggcaattga aaaatatgaa caactcgttg
     6241 cagacaatac catttcattt gaagtttatt ataatctggg taatgcatat tacaagacag
     6301 gcaacttcac aaaagcaatt ttaaattacg aaagagcaaa aaaaattaaa cctgacgatg
     6361 ctgatgtcaa cctcaattta gcgatagcta atcaaaaaac aattgacaaa attgaaccaa
     6421 caccaaaagt attttacaat cagtggtggg ataattatat agcctcctcc acggcagact
     6481 catcggctgt cattatgtta ttttttcttt ggttgtttat tgcagcaata gccatcatga
     6541 ttataattaa aagtgcttca ttgaaaaaat tatgttttat catcagttta attctggctt
     6601 gctgcacttt gtttttcttt atcacaacac aaacacgcca aaacataaac gactcatctg
     6661 tggaagcatt aatcataagc caaagcgctt atatcaaaag cgcgcctgat gataaaggca
     6721 ccaacttgtt ccaattacat gaaggaacga aaattaatat tgttgatgaa ttgagttctt
     6781 ggaaaaaaat aagaatcccc aatgggaata ttggatggat taagtctaaa gatctggaaa
     6841 caatttaatc accattgctg tcgctcatac cagcgatgaa gtacgattaa gcaccagata
     6901 cgattatata aatagtcttc tccggaaaga aatcgttgaa ttaacttttt tacaacttca
     6961 aatttcacta atcctgcatg tcgaattgtt ttttcatcaa gataacgatt aagcaaatat
     7021 ttccaatcag cttttaacca ttttgcaaga ggaatagaaa atcctttttt gggtcgcata
     7081 aataattctt caggaacata attggcaaga atttttttta gtggatattt gtttattcca
     7141 ttcttgattt taatgtcact ggaaactgag aaaacatatt cagcaaaatt attgtctaaa
     7201 aaaggcacac gtgcttctat agaatgaaac atagtagctc tatcaacttt ggttagcaaa
     7261 tcgtcctgaa atggaaattt tagctcataa tttgcctgtc tttcctctgg caaacaactg
     7321 tcattaatat taaacaactt ttccaattct tcatttcttt gtgcaatatt cttcaaggtc
     7381 tctttcctta attcatcaga aaccaattcc actatttctt tgttactgaa caagtattgt
     7441 tcttgtgaat aaatgtgttc aggcaaaaaa tcggaatgct cattaaaatc caataattgt
     7501 gcaacacgtt tactgcgtga gttaccacaa gtaagaatag ttcggatagg acttctgaaa
     7561 acgctaaaag ggaaagcatt taatcgctta gcccaattat gggtaccata gccgaaaaac
     7621 aattcatcac cgccttctcc tgacaacact acctttacat gctttgaagc aagttttgag
     7681 actaacattg taggaatacc agaggtatca gtataaggtt catcataaac ttcaaaaaaa
     7741 gtttcaatta aatcaattgc atctttaaca gaaacaacaa actcatgatg atttgttcca
     7801 atgtgttttg caatcactct tgcatgttct gattcattat ggctttgttc agcaaaacca
     7861 attgaaaatg tattcagttt ttcatcaacg tttcttgctg ctattgcagt aagcaaactt
     7921 gaatcaacac caccacttaa aaagactcca taaggaacat cgctgcgcaa ttgaatcttt
     7981 accgattgta tcagaagttt ttcaatgttt tgaattgcct ttttttcatc gtgttcaatt
     8041 tctcgttgtg aacattttct attcatcaca aacggatgaa tctccaaacc gtttctgtca
     8101 atagtcaaga aatttccgct ttcaagctta aatactttct catacatcgt taatggtgca
     8161 ggaacaaatc cagtatgaag aaaatatgct acagcctgac tatttacttt tctttcaata
     8221 ctatcaattg caagaattgc tttaagttca gatgcaaaac aaaaatttcc tgatgaatca
     8281 tgatagtaat ataaaggttt tatcccaaat ctatctctaa acaaaaataa tttgttggct
     8341 tgtgtgtcat aaatggcaat tgcaaacatg ccattcaaat ctttaacaaa atcaacacct
     8401 tttatctcaa acaatcttaa aataacttct gtgtcacttg tagttttaaa atcagacaat
     8461 tgactgtctt tatattcaga ctcaatctct ttacgtaact ctacataatt atagacctcc
     8521 ccattataaa ccatcacata acgcatgcag gaagaataca taggctgtga tgctctattg
     8581 tcgagatcaa ttattgataa cctgcgatgt gccaatcgga caaaattatc actgtaaaat
     8641 ccatctccat ctggcccacg atgcttaatg gcagcagatg ccttcctgaa atcctgctca
     8701 gaaaaaaaat tgcttaccga aaaactacct gctattccgc acatcagctg ataaatattt
     8761 ttgctattgc cttataaaaa tgcatatccg tatatgttga agggtacttc agaaccttca
     8821 ttaattctgt taatcccttt cgggtataat tggatgacag ataaacatta cttacaaaaa
     8881 gctgaagata ttgttgcatt ttggctttaa tatttgttgg aagagaaaaa ttatcaataa
     8941 aataatttgt agcaaattta ttccaataaa caaactgttc agtggaaatc agatttacag
     9001 acctattaac atgtgagttt acaaaatttg taatgatacc tgatttcaga attttatatc
     9061 tgaaactgaa catacgaaag aacaacaaat cctcagccgt aagaatattg atatcaggaa
     9121 aaagtagttc aggataatgc gactttttat aacaaaactg atttaatgaa ataggattag
     9181 cttctgcaaa atcgctcaaa ctgaaaactc tttgatctct ataataatta caatttgcat
     9241 agtctcttat tttgctttca acaaccaatt cagttgcaat gcctgaaaag gaaggaaact
     9301 cctgtactaa tttatacatt gttgaaagat gattctcaag ataataatca tcagagtcaa
     9361 gaaaggcaat ccaatcaccg gaagctgcat ttattccatt gtttcgggca gcacaacgac
     9421 ctttattctg atgtgaaatc aatttaatac gcctatctat ctttgaaact tcctttaaaa
     9481 cttgcaatgt attatcagtt gaaccatcat caatacaaat tagttcaaaa tctttaaaac
     9541 tctgattaag aacagagaca attgaatctt taaccaaatc tgcacgattg tacaccggca
     9601 taatgacaga aaaaaaggga ggcttatcag tcaacataaa tttgtccttt cttaacacct
     9661 cttttaagtc ctacataata acatagcatt tctttaaaaa aaactccggc tccaataagt
     9721 cgaaaagctt tcaacaagcc catgaaccca atttgccaat tgataaataa aagataaaat
     9781 ctaagtcggt gatctttaac tatatttcta atcagataac aaacaattaa tgtttgctgg
     9841 tcgaaatatg ctttagtgtg tatcggatac cttaatgtac aaatatttgt aaatctgttt
     9901 atacgcacca taccagaact acaaaccaat atcaactcaa agagggtatc aaaatgagaa
     9961 tccactttaa aatcgggaaa tacattactc ttcaataaac tgctatgaat catcaactgt
    10021 gaaaggctta actgctcctt taactcaatc ttcttaacat tgattatatt atcttcagga
    10081 cctacagatt ttatagtgct ataacctctc agataattta atgttaaata tgcctggaaa
    10141 tctttaaaat gtagcatagc atcacgaaat gccgacaaat gatatggcag ccactcatca
    10201 cccggcttaa gaagacaaat ccatcctgct tcaacagttc ttaagatgcc aac
//
LOCUS       DAOOOI010000072         9519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1412_length_9519_cov_10.730241, whole genome shotgun sequence.
ACCESSION   DAOOOI010000072 DAOOOI010000000
VERSION     DAOOOI010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1412_length_9519_cov_10.730241"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2225)
                     /locus_tag="PKX59_08565"
     CDS             complement(<1..2225)
                     /locus_tag="PKX59_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90958.1"
                     /translation="METQLHTDAKGNPVAPELFVAEIIKATQEKEDVLSNKTKLVNSW
                     LPAGPFNQPINQTGYLEVGMGRINCIAFHPTDPATYFVGVAQGGVWKTTNNGQSWIPL
                     TDNLPILRISDIAIDPLNPNIMYISVCDYAYIDVNLQTSGRKRNTHYGLGLYKTIDGG
                     LTWQPSGLSFQLTNGDASLLRKTIIHPTQTNKLVVCGVSGMYTSNDAGANWTKTMDSL
                     FWDLVQDPINPNILYAASGWLNNTNIGNAAIYKSIDFGITWSMLPTGIPSTGAVQRIK
                     LAIAPSDNNYIYAITVDIDRGLYGFYKTTDAGASWQFIPPSLNILEGGQGTGIGGQGT
                     YDLGLCVNPNNRDEIYAGGVNMWGSIDGAQSFNPISHWTTSFGPTTHADIHSIVVQPL
                     TGNFFVCNDGGLYRTNAITIHNWNDANSGIPWPTQWTKLNDGLQITSFYRLSSAKNTS
                     GKLMAGAQDNSTYFFNGSGWTNIFGGDGMDNYINPLNDSNIIGSSQFGNFDLSFDNGV
                     NSTWCNPNVNGELGEWTTPLVADYANNGTLYAGFENVTKTTDGGNSWNAISTFPVSGF
                     ANNEISTLAVSATNPDVVYAGKRVRYEFGVPASLYTTSNGGVVWNDVTAGIPDSLYYT
                     SLDVNAVNSSIAYISMAGFSVGNKIFRTMDGGATWQNISYNLPNLPVNCVKSLPGGKM
                     LLAGTDIGVYLLEDTLSNWINISNGLPNVIISDIEVNQAMNRLYVSTFGRGIWETDLD
                     V"
     gene            complement(2539..2697)
                     /gene="rpmH"
                     /locus_tag="PKX59_08570"
     CDS             complement(2539..2697)
                     /gene="rpmH"
                     /locus_tag="PKX59_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOZ90959.1"
                     /translation="MKRTFQPSQRKRRNKHGFRERMSTANGRRVLNARRAKGRKKLSV
                     SSDKLHKR"
     gene            complement(2797..3213)
                     /locus_tag="PKX59_08575"
     CDS             complement(2797..3213)
                     /locus_tag="PKX59_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOZ90960.1"
                     /translation="MKLFLDANILISVFNNEYPLFTYSSRILSLTDNKKFEVYTSPLC
                     LAIAYYFAEKKSGGNLAKQKITLLSQKIKIAAQTETCVKESLSNKKIHDFEDGMQYYA
                     AIHAKCKCIVTQNVDDFYFSEIKILDAKSFLEQTFL"
     gene            complement(3210..3467)
                     /locus_tag="PKX59_08580"
     CDS             complement(3210..3467)
                     /locus_tag="PKX59_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HOZ90961.1"
                     /translation="MDTKITLSFDEQVIAKAKKFADENNISLSRLTEFLLAKVTSSTY
                     KSLDSLPVSEWISILSEGQAEYQTKAKTRKALRNEYYSSKK"
     gene            complement(3560..5200)
                     /locus_tag="PKX59_08585"
     CDS             complement(3560..5200)
                     /locus_tag="PKX59_08585"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOZ90962.1"
                     /translation="MIRNRFFCLLLLLSCCTYIVPAQTRADLLLFNGKIYSVDSGFTM
                     YEAMAVKDGKIIAMGTTKYLATYPAKEKLDLKNNYVYPGFIDAHCHFYGYGVDLKKIN
                     VTGTKSWESVLDSLKVHKEQKFMGWIFARGWDQNDWEIKNYPTKEKLDSLFPDTPVFI
                     LRIDGHAALVNQMGLNKAGIEISTKVEGGEIEVKNGELTGLLIDNAVDLVKLKIPNPT
                     REAEIEALLQAQKNCFEVGLTTVDDAGLEKETILLIDSLQKAGSLKMRYYAMITYNDA
                     NKNYFFKKGKIKTERLSACAFKIYADGALGSRGALLSKPYSDHENHIGLYLTPIEKLK
                     EAAIEIAAHDFQLCTHCIGDSSNRYVLNLYATATKNKTNARWRIEHAQVLNAEDFPLF
                     KQGNIIPSVQPTHATSDMYWAEDRLGKERIKNAYAYKKLLAVSGIIAAGSDFPVEDIN
                     PLYGFYAATVRKDKNNFPKDGFQIENALSRQEALKAMTIWAAYANFEEKEKGSLEIGK
                     YADFVILKENLLELEEEKLWQIKVQSTFVNGERVFERK"
     gene            complement(5287..5360)
                     /locus_tag="PKX59_08590"
     tRNA            complement(5287..5360)
                     /locus_tag="PKX59_08590"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5324..5326),aa:Pro,seq:ggg)
     gene            complement(5470..7794)
                     /locus_tag="PKX59_08595"
     CDS             complement(5470..7794)
                     /locus_tag="PKX59_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ90963.1"
                     /translation="MSILKTNAQVATQIVRGQVVDKITQSPLPGATVMLLNHTPQLGT
                     AADADGFFKLREVPLGKQSIVVNYIGYKPSTLPNITVNAGKEVVLIISLEENTIVKKE
                     FVVTAKVEKNKALNELATVSARAFSVEETQKYAAAVNDPARMVTAFAGVACTDDGNNK
                     IAIRGNAPNGLLWRMEGVEIPNPNHFSNVGTSGGGVSILSSQLMTNSDFLTGAFPAEY
                     GNALSGVFDIKLRKGNDEKREYTVQAGVLGLDAAAEGPFKKGYNGSYLINYRYSTLSV
                     LGKIGVPLGDAITLFQDLSYNISLPTKRLGVFGLFGFGGISNQKTWSTKDSLKWKEDS
                     FKKYDTEFTANTGASGLTHTLLLNPNTALRSALVFSGASNGYHETELDSFYEKIEQRR
                     QNFVQNKLTFSSTLTKKFNAKSSLKSGIILNSIAFKLTQKYFDQEQGKYLVALNTKGN
                     AHTLQAFSQLSYKLTEAFTVHPGVHYITYLENNSHSVEPRLSARYQVNRNNSVSMGYG
                     MHSQMQPIGVYFGESTDEYGTTTRPNKNLKLNKSQHAVLSYDLLVGAFHHIKLETYFQ
                     YLTNVAISADTTSSFSLLNLEEGYYTKPLNNSGIGRNYGLEITAERFLKNNFYYLLSV
                     SLFDSKYRAADNKWYNTRFNGNYLGTFTVGKDIPFERKGKSRVFGLGAKIVYSGGLRE
                     TPINSELSIAKGDAVYEENKAYSKQNPAYFRLDLKLSLKRNYKRSTGTVAFDIQNASN
                     QKNIGGTYYDKNNAKVDTWTQAPLIPILSYRIEF"
     gene            complement(7923..9002)
                     /locus_tag="PKX59_08600"
     CDS             complement(7923..9002)
                     /locus_tag="PKX59_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90964.1"
                     /translation="MKNDYSLNILGGYSLGTRKIEVGGVLNINRSETGKLQLAGLANF
                     VGGKVSGIQMAGLTNLNQKGFKGIQSAGFLNMNQDSCKGIQLAGFLNLNKCTMEGFQA
                     AGFANINAASEQGVMLSGFSNMNKGNYTGVQAAGFGNSHKGGFYGVQASGFYNSTKGS
                     FEGLQLAGFLNYAQTIKGVQIAFINFSDSCKGVPIGFFNYVKSGYYRLELATDEQLNI
                     HISLRSGTQQFYTLLAATSQVTSLQGKKDIWSFGYGLGTAFQVGKKMQVNLDLIAHQY
                     ALGNSLALNQNYGNLLLNLDYRIAKRISIFVGPVLNVALLTPSDTKNYTTLSNNVPSY
                     AKTDNLTTSHQLRYWLGGKCGIRFL"
     gene            complement(9137..>9519)
                     /locus_tag="PKX59_08605"
     CDS             complement(9137..>9519)
                     /locus_tag="PKX59_08605"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOZ90965.1"
                     /translation="LNIIFSNKVSFKTRGNYIILQKKTANEILTQKKNVVVQGYVTDA
                     KSGQKIKNATIYDKKSLGSATSDAFGFYEIKLKNERELQLNVNKNNYTDTILFVSQQP
                     DEMINVAIYPTKTDSAQQLAYFQK"
BASE COUNT         2758 a         1894 c         1677 g         3190 t
ORIGIN      
        1 attacatcca aatctgtttc ccaaattcct ctcccaaaag tcgatacata caatctgttc
       61 atagcttgat ttacctcaat atcactaata atcacattcg gaagtccgtt actaatgttt
      121 atccagttac ttaaggtatc ttccaaaaga taaacgccaa tatcggtgcc tgcaagcaac
      181 atttttccac ccggtaaact ctttacacaa tttactggaa gattaggaag attataggaa
      241 atgttttgcc aagttgctcc accatccatt gttctaaaaa ttttgtttcc aacactaaat
      301 ccggccatac tgatataagc aatgctcgaa ttcacggcat tcacatccaa ggaggtataa
      361 tacaaagagt cgggaatgcc tgctgtaaca tcattccaaa caactcctcc atttgaggtg
      421 gtatacaaac ttgccggtac accaaattca tatcgcaccc gttttccagc atacactaca
      481 tccggatttg ttgctgatac agccaaagtg gaaatttcat tgttagcaaa tccacttacc
      541 ggaaaagtgg atatggcatt ccatgaattt cctccgtcag tagtttttgt aacattttca
      601 aatcctgcgt aaagtgttcc attgttggca taatcggcca ccagtggggt tgtccattcg
      661 cccaattccc catttacgtt tggattgcac caagttgaat tcacaccatt gtcaaatgat
      721 aaatcaaagt ttccaaactg actagaacct ataatattac tatcgtttaa aggattaata
      781 taattgtcca tgccatctcc cccaaaaatg ttggtccaac cactgccatt aaaaaaatag
      841 gtggaattat cttgagcacc tgccatcagt tttccggaag tgttttttgc actcgaaagc
      901 cggtaaaagg aggttatttg taaaccatca tttaatttgg tccattgggt gggccaagga
      961 attccactat ttgcatcatt ccaattgtga atggtaattg cattggttct atacaaacct
     1021 ccgtcattac aaacaaaaaa attaccagta aggggttgaa ccactataga gtgaatatca
     1081 gcatgcgtag tgggcccaaa gctggttgtc cagtgactta ttggattaaa actttgtgcc
     1141 ccatcaattg aaccccacat atttacacca ccggcataaa tctcatcgcg attgtttgga
     1201 tttacacaaa ggccaagatc atacgttccc tggcctccaa ttcctgtgcc ttgtccacct
     1261 tcgagtatat ttaaactcgg tggaataaac tgccaagaag cacctgcatc ggtagtttta
     1321 taaaaaccat acaagcctcg gtcaatgtca acagttatcg cgtaaatata attattgtct
     1381 gagggagcaa tggcaagttt tatgcgctga acagcaccgg ttgaaggaat accggttggt
     1441 agcatgctcc aagttatgcc aaagtcaatg gatttataaa tagctgcatt tccaatattg
     1501 gtattgttca gccaaccact ggcagcatag agtatattag gattaatagg gtcttgaacc
     1561 aaatcccaaa agagggaatc catcgttttt gtccagtttg cccctgcatc gttgctggta
     1621 tacattccgc tcacaccaca caccaccagc ttatttgttt gagtaggatg aatgatggtt
     1681 tttcgaagca aagaagcatc tccattggtg agttgaaaac ttaaaccgct aggctgccag
     1741 gtgaggcctc catcaattgt tttatacaat cccaaaccat aatgcgtgtt gcgttttctg
     1801 ccgcttgtct gcaagttcac atcaatataa gcataatcac aaacagagat atacataatg
     1861 ttaggattaa ggggatcaat tgcaatatca cttattcgta aaatgggtaa attgtcggtt
     1921 agcggaatcc aggattggcc attgttggtt gttttccaaa caccgccttg tgccacacct
     1981 acaaaatagg tagccggatc tgttggatga aaagcaatgc aattgatacg gcccatacct
     2041 acttccaaat aaccggtttg attaatgggc tggttaaaag gacctgccgg caaccaagaa
     2101 tttacgagtt tagttttatt ggaaagaaca tcttcctttt cttgtgtggc ttttatgatt
     2161 tcggcaacaa aaagctcagg agctaccgga tttccttttg catccgtatg gagctgcgtt
     2221 tccatttccc aacgcttaaa atttttccag tgtttttgtt gactttttgg aacagttctc
     2281 ttccaagtat caaacttaag ctgcatttct ttgaaagaga ggggagctgc tgaatttctt
     2341 tttaaaaaat cttgagctgt gagttgattt gtgaagagaa taagcaaaag aaccggaagt
     2401 aatcgtgaat actgcatttt tagctaggtt ggattgggtt attgaatttc aaatgtaaac
     2461 aatataaaat aaaaatgcta ctcattattg aaaacgagta gcatttatta ctatagattt
     2521 gtagctgact gctaaaaatt atctcttgtg taatttatca gaggatacag aaagcttttt
     2581 acgtcctttt gctctacgag cattaagcac tctgcgccca tttgctgttg acatgcgctc
     2641 acgaaaacca tgtttgtttc ttctctttct ctgggaaggt tgaaatgtcc ttttcatcgt
     2701 attgtatatt tactttttta aaacggactg caaaggtaaa aaaaatacca ttccgtcaaa
     2761 atatttttta gcttcctttc aatttaaagc gctgcattac aaaaatgttt gctccagaaa
     2821 gctttttgca tccagtattt ttatctctga aaaataaaaa tcgtccacat tttgtgttac
     2881 aatacatttg catttggcat gaatagcagc ataatattgc attccatctt caaaatcgtg
     2941 aattttttta ttagaaaggc tttcctttac gcaagtctct gtttgtgcag caatttttat
     3001 tttttgtgat agcaaagtga ttttttgctt cgctaagttg cctccacttt tcttttcggc
     3061 aaaataatag gcaatggcca aacaaagtgg actggtataa acctcaaact ttttattgtc
     3121 agttaagcta agaatgcgag aagaataggt aaataatgga tattcattat taaacacgga
     3181 aataagaatg ttggcgtcta aaaatagttt cactttttag aactataata ttcatttctc
     3241 agtgctttgc gtgttttagc tttcgtttga tattcggcct gtccttctga aaggatagaa
     3301 atccattctg aaacaggaag gctatcaaga gatttataag tactactagt tactttagcc
     3361 aataaaaatt cagtaagccg cgaaagacta atgttgtttt catctgcaaa tttcttggct
     3421 ttggcaatta cttgctcgtc aaaactgagt gttattttgg tgtccatttc ttatttatac
     3481 cacaaaaata ttattttttt acgtacaatc aaactcttct ctattttttc aacatttatt
     3541 gcgctttcgt aacacaccat cattttcttt caaacactct ctctccattt acaaaggtgc
     3601 tttgcacttt tatttgccaa agtttctctt cctccaattc caataaattt tctttaagta
     3661 ttacaaaatc ggcatatttt cctatttcaa gtgagccctt ttctttttct tcaaaattag
     3721 cataagcagc ccaaatggtc attgctttta gcgcttcttg acgagaaagt gcattttcaa
     3781 tttgaaagcc gtcttttggg aaattatttt tgtcttttcg tacagtggca gcataaaatc
     3841 catacaaagg attaatgtct tcaaccggaa aatcactgcc tgcagcaatt ataccggaaa
     3901 cagccaaaag ctttttgtaa gcataggcat tttttattcg ctcttttcct aacctatctt
     3961 cagcccaata catatcgctg gtagcatgag tgggttgaac cgatggaatt atatttcctt
     4021 gtttaaagag aggaaagtct tctgcattta aaacttgcgc atgttcaatt ctccaacgtg
     4081 cattcgtctt gttttttgtt gcagtagcat aaagatttaa tacataacgg ttagatgaat
     4141 ctccaatgca atgcgtgcag agttgaaaat catgtgcagc aatttcaatt gctgcttctt
     4201 ttaatttttc aatgggtgtt aaatacaagc caatatggtt ttcatgatct gaatatggct
     4261 ttgataatag cgcacctcga gaacccaatg caccgtcggc ataaatttta aaggcgcaag
     4321 cgcttaatct ttcagtttta atttttcctt tcttaaaaaa ataattttta ttagcatcgt
     4381 tataagtaat cattgcataa taacgcattt taaggctgcc ggctttctgc aaagagtcaa
     4441 tcagtaatat ggtttctttt tctaatccgg catcgtctac agttgtgaga cctacttcaa
     4501 agcagttttt ttgcgcttgc agcaaggctt caatttctgc ttcacgtgtg ggatttggaa
     4561 tttttaattt cactaaatca acggcattgt caatgagtag cccggtcagt tcaccatttt
     4621 taacctctat ttcaccaccc tcaacttttg tcgaaatttc aattcctgct ttgtttaatc
     4681 ccatttgatt taccaatgct gcatgcccat caatgcgtag aataaataca ggagtatctg
     4741 gaaaaaggct atctagtttt tcttttgtag gatagttttt aatttcccaa tcgttttggt
     4801 cccaacctcg tgcaaatatc caacccataa atttttgctc tttgtgcact ttcaaactgt
     4861 ctaaaacaga ctcccaagac ttggttccgg ttacatttat cttttttaaa tccacaccat
     4921 aaccataaaa atgacaatgt gcatctataa agccggggta tacataatta ttttttaaat
     4981 ccagtttttc ttttgcagga taggtagcaa gatactttgt agtgcccatg gcaataattt
     5041 tcccatcctt tactgccatt gcttcataca tggtgaagcc agagtcaaca gaataaattt
     5101 ttccgttgaa aagcaataag tcggccctcg tttgagcagg tacaatatat gtgcagcagg
     5161 agagtaggag aagtaggcaa aaaaaacggt tacgaatcat ggcttcaaga actattttga
     5221 gattaaaaat cagctaaaag tatcaaaaaa aaaagcctga atagtttcag gcttctaaga
     5281 attgtgtcgg gatgactgga tttgaaccag cgacctccag caccccatgc tgacgcgata
     5341 ccgggctacg ctacatcccg aacttttttg gaacgggtaa tgttccgctt caaaattaaa
     5401 ttaaatttcc aaaagtttac tacaggcatt taaagaaatt tcaattttga attcaatgag
     5461 tgaagcgtat taaaattcaa ttctgtaact taatatcggt ataagaggag cctgcgtcca
     5521 tgtatcaacc tttgcattgt ttttatcata ataagttcct cctatatttt tttggttact
     5581 tgcattctga atgtcaaaag caacagtacc agtgctgcgc ttgtaatttc ttttcaaact
     5641 cagcttgagg tccagtctga agtaagctgg attttgttta gaatacgctt tgttttcctc
     5701 atatacagca tctccttttg caattgataa ttctgaattt atgggtgttt cacgcaagcc
     5761 tccgctatac actatttttg cccctaagcc aaaaacacgc gatttacctt ttctttcaaa
     5821 aggaatgtct ttccctaccg taaatgttcc taaatagttt ccattaaaac gagtgttata
     5881 ccatttatta tcagcagcac ggtatttcga atcaaacaat gagacgctca gtaaatagta
     5941 aaagttattt ttaagaaatc gttcagcagt aatctctagt ccataatttc ttcctattcc
     6001 actattgttt aaaggtttag tgtaatagcc ttcctctaaa tttaataaag aaaaagagct
     6061 agttgtatct gcacttatgg caacattagt taaatactgg aaataagttt ctagttttat
     6121 atggtgaaag gctccaacta ataaatcgta gctcaatact gcatgttgtg atttatttag
     6181 ttttaaattt ttgttcggtc gtgttgtagt tccatattca tctgtgcttt cgccaaagta
     6241 aacaccaatc ggttgcattt ggctatgcat cccatatccc atgctcacac tgttatttct
     6301 atttacctga tatcgagccg atagtcgggg ttcgaccgaa tgtgaattgt tttcaagata
     6361 ggtgatgtaa tgtacaccgg gatgaactgt aaaagcctcg gttaatttgt aactcaactg
     6421 actaaaagct tgcaaggtat gtgcatttcc tttggtatta agggctacta aatacttacc
     6481 ttgctcttgg tcaaagtatt tttgagtcag tttaaaagca atgctgttaa gtataatacc
     6541 actttttaaa ctgctttttg cattgaattt tttggtaagg gtgctgctga atgtcaattt
     6601 attttgaacg aaattttgcc gtctctgttc aattttttca taaaacgaat ccaattctgt
     6661 ttcatggtat ccatttgatg cacctgaaaa gactagtgca gagcgcaaag ctgtattcgg
     6721 atttaaaagc agtgtgtgtg ttagtcccga tgcacctgta ttggcagtaa attcagtatc
     6781 atatttttta aaggagtctt ccttccattt taatgaatct tttgttgacc aagttttttg
     6841 attgcttatc cctccaaatc caaaaagacc aaatactccc aatcgtttgg taggcaagga
     6901 tatgttgtat gataagtctt gaaacaaagt aatggcatca cccaaaggca caccaatttt
     6961 gcctaaaaca gataaggttg aataccgata atttataagg tatgaaccgt tgtaaccctt
     7021 tttaaatgga ccttcagcag cagcatcgag ccctagtaca ccggcttgta cagtatattc
     7081 tcttttttca tcgttgcctt ttcttaactt aatatcaaaa actccggaaa gggcattgcc
     7141 atactccgca ggaaaagctc cggttaggaa gtcggaattg gtcataagtt gtgaacttaa
     7201 aatggaaaca ccaccgcccg atgttccaac attgctaaaa tgattcgggt tagggatttc
     7261 taccccttcc atccgccaca gcaagccatt gggagcattt ccacgaatcg ctattttatt
     7321 gttcccatca tccgtgcaag caaccccggc aaaggctgta accattcgag ctgggtcatt
     7381 tactgcagcg gcatattttt gcgtttcctc caccgaaaag gcccgtgcgc taaccgttgc
     7441 cagttcattc agcgctttgt ttttttcaac tttggcagtc acaacaaatt ctttttttac
     7501 aatcgtattc tcttctaaag atataatcaa tactacctcc ttgcctgcat ttaccgttat
     7561 gtttggaagc gtacttggct tgtagccaat atagtttaca acaatgcttt gttttcccaa
     7621 tggtacttct cttaatttaa aaaagccatc tgcatcggct gcagttccca gttgaggagt
     7681 atgatttaat agcattacag ttgctccagg caaagggctt tgtgtaattt tatctaccac
     7741 ttgcccgcga acaatttgag ttgcaacttg tgcatttgtt tttaaaatgg acatgaaaag
     7801 tagcaagctt cctgccatta gatgttgtct tgtcataaca atttttatgt ttgtttaatg
     7861 ctaagaccag caaatggatg tgtaccccta tgcggctttg attttttttt tgtgaggcat
     7921 ttctagagaa agcgaatccc acatttaccc ccaagccaat agcgaagttg gtgagaagta
     7981 gttaaattat ctgttttagc gtaagatgga acattgtttg aaagggtagt ataattcttg
     8041 gtatcggagg gggtgagcag tgctacattc aaaaccggac caacaaaaat tgaaatcctt
     8101 ttggctatcc tgtaatctag gttgagcagt aggttgccat aattctggtt caacgcaagg
     8161 ctatttccca aagcatattg atgagcaata agatctaaat tcacttgcat tttttttccg
     8221 acctgaaatg ctgttcctaa accatatcca aaagaccata tatctttttt accttgcagg
     8281 ctagtcactt gtgaggtggc agcaaggagg gtgtaaaact gctgggtacc acttcttaaa
     8341 gaaatatgaa tattcaattg ttcgtctgtt gcaagttcta agcgatagta gccgcttttt
     8401 acataattga aaaatccaat tggaactcct ttgcaggaat cagaaaaatt aatgaaagca
     8461 atttgcacac cttttatcgt ttgtgcataa ttcaaaaatc ctgcaagttg caaaccttca
     8521 aaagaaccct ttgtggaatt gtaaaatcct gaagcttgta ctccataaaa acctcctttg
     8581 tgagaattgc caaaacctgc tgcctgaaca cctgtatagt ttcctttatt catattgcta
     8641 aaccccgaaa gcataacacc ttgttctgag gccgcgttga tattggcaaa accggcagcc
     8701 tgaaaacctt ccatagtaca tttatttaaa ttgagaaacc cggcaagctg aattcctttg
     8761 cacgaatcct gattcatatt taaaaagcca gcactttgaa ttcctttaaa tcccttttga
     8821 ttaaggttcg ttaaacctgc catttgtata cccgaaactt ttcctccaac aaaattggct
     8881 aaaccggcca gttgtagttt tccggtttcg cttctgttaa tatttaatac tcctcccact
     8941 tctatttttc ttgttcccag ggaatagccg ccaaggatgt ttaacgaata atcatttttc
     9001 acacttccgc ttaacagctt attcgtgcca atatagggta ccagtgaaac ttgccacttg
     9061 cgtgacaggg tatcctttac atttaaacta tttacttttt gtttggcatg tataaaaaaa
     9121 tctactgtag agttttttac ttttgaaagt atgctagctg ttgggcactg tctgtttttg
     9181 ttgggtatat cgctacattt atcatctcgt cgggttgttg tgaaacaaat agtattgtat
     9241 cggtataatt attcttgttc acgtttaact gcagctctcg ttcatttttt agttttatct
     9301 cataaaatcc aaaggcatcg ctagtggctg acccgagtga ttttttatca taaatagtag
     9361 cattcttaat tttttggccg ctttttgcat ccgttacgta tccttgcaca accacatttt
     9421 ttttctgcgt taatatttca ttggctgttt tcttttgcag gataatgtaa ttgccacgtg
     9481 ttttaaatga cactttattt gaaaaaataa tgttcagca
//
LOCUS       DAOOOI010000073         9364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1442_length_9364_cov_21.943496, whole genome shotgun sequence.
ACCESSION   DAOOOI010000073 DAOOOI010000000
VERSION     DAOOOI010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9364
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1442_length_9364_cov_21.943496"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /gene="hppD"
                     /locus_tag="PKX59_08610"
     CDS             complement(<1..1020)
                     /gene="hppD"
                     /locus_tag="PKX59_08610"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006795772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HOZ90966.1"
                     /translation="MATNDLTTVKNFNYSENKKIFEQAQDFLPLLGTDYVEFYVGNAK
                     QAAHYYKTAFGFQDLAYAGLETGVRDRTSYVLQQGKIRLVLTTPLTSSNPIADHIKKH
                     GDGVKVIALWVDDATDAFEQTVKRGAKPYLSPEVLKDKDGEVRRSGIHTYGDTIHIFI
                     ERKNYKGIFLPGYEKYQSDYNPPSTGLKYIDHMVGNVELGGMNVWSKFYAEVMGFFNL
                     ITFDDKDISTEYTALMSKVMTNGNGYIKFPINEPAIGKKKSQVEEYLDFYEGAGCQHI
                     AVATDDIVHTITEMRKRGVEFLYVPGSYYDTVKERVGIIEEDLEELKKWGIMVDRDEE
                     GYLLQI"
     gene            complement(1109..2284)
                     /locus_tag="PKX59_08615"
     CDS             complement(1109..2284)
                     /locus_tag="PKX59_08615"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HOZ90967.1"
                     /translation="MPIYQRQGKTPHKRHIVFRKKDGSLYHEELFGTEGFSGISSLVY
                     HLNPPTMVKEHGKPYSVRPEIAVEDNLQARSFLGFEVKPENDYLKSRKVLFVNDAMHI
                     GLASPTKGTTDYFYKNADADEMLFVHRGSGTLKTLFGTIDFEYGDYLIIPRGIIYQIN
                     FTTADNKLLFIESHAPIETPARYRNQYGQFMENSPFCERDFKLPHSLETHDERGDFLI
                     NIKKRGLIYPYIYETHPFDAVGFDGCFYPYGISIFNFEPITGRIHMPPPIHQQFQSSN
                     FVVCSFVPRLYDYHPQAIPAPYHHSNMDSDELLYYVDGDFMSRNNIRQGQITLHPGGL
                     SHGPHPGAIERSIGKKETNELAVMIDPFNPVKITQAATEIEIEEYYQSWRMHESETI"
     gene            complement(2471..3091)
                     /locus_tag="PKX59_08620"
     CDS             complement(2471..3091)
                     /locus_tag="PKX59_08620"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOZ90968.1"
                     /translation="MYHPSIINEATYATLKKVFEVDDVKKHFALAGGTSLALQLKHRH
                     SIDLDIFSTVPFDTYVFERVLTNESGFIFERVNSNKNMLFAYINSIKCDFIYEPSKLL
                     QPFLVKDTISYFSIADITAMKMHTICGRRKKKDFFDVYVLLENFGWEQMLQWFVNKYD
                     KSQLYFLYRSIHYFEDANEDPDITGIAPYTKNWDEIKEFLHISCKI"
     gene            complement(3069..3392)
                     /locus_tag="PKX59_08625"
     CDS             complement(3069..3392)
                     /locus_tag="PKX59_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90969.1"
                     /translation="MNSFLKYIDTTKLDKKIKEERFPFSQQLFWDYPIKDIDLKIHQR
                     FIIERVLTRGFTNDFYILQKIYSIDEIQAALRKSKELDPKTAHFCSWYFNIPLHELHV
                     SSFYH"
     gene            3679..5268
                     /locus_tag="PKX59_08630"
     CDS             3679..5268
                     /locus_tag="PKX59_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90970.1"
                     /translation="MKNRNLPNQDYKLITLPNKMIKVLVDGKFAQLNTVGMEQVFHAL
                     MFLLAYPQSEKQEVFALKKLNTVAAIIKNKAAAFKNKLINSGLPFTNMSVQFTHDFLQ
                     WLLQQKELKVKFRQFVNSETDLNELLKLTLNPAEKSETTAGLSNEDLLHSLGIEQHEV
                     LNFLLDEFSKIYQPEVKDYLFDSVGLQCDLIPQNKFFSLPYNRFTRNKTFYHEEILKK
                     FDYKNVLDSEINPAVSLSENEHVELIKTIKYAMVLTNRETDTVTYMDENTLQYFELER
                     GISVAIYGMTPDRQLPFESYIGYTLFKNGFPCAYGGSWVFGKRALFGINIFEWFRGGE
                     SGYVLCQLLRVFHKVFDVDYFEVEPYQYGLDNPDGISSGAFWFYYKFGFRPVDKELKK
                     IAEREFHKIKSKQNYRTQEKTLLRFTESNISLTLEKINQAKIVEVTSVISGMIKKQFK
                     GNRMQAEQECVSLFLQKAQRAVNHSEYSEALLEMALLCEAYKIKDDETISLLYAMAHI
                     KPVNPYDYQGILLKFLEKIAK"
     gene            complement(5358..5624)
                     /locus_tag="PKX59_08635"
     CDS             complement(5358..5624)
                     /locus_tag="PKX59_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90971.1"
                     /translation="MGISNIEQGVLNIYPTVTTGNINLESSNERKAISIAVINMIGKE
                     VKRLNFIQNNGKTILNLSELPDGVYVLKINMNGQAINKRIVIRR"
     gene            complement(5730..6125)
                     /locus_tag="PKX59_08640"
     CDS             complement(5730..6125)
                     /locus_tag="PKX59_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90972.1"
                     /translation="MIKRLLTIAFAVLTTNVFSQVLVSYDFTGYDGLVTSIPAGWTIT
                     NNDTTAVGKTFYNTPTYCGISCNSYKFNVTGATIISPSFSNATHLKFYMKGNGNTQQP
                     NPFKIYTTADGTNWNNIQTYNPVSQTGAF"
     gene            complement(6398..6964)
                     /gene="pth"
                     /locus_tag="PKX59_08645"
     CDS             complement(6398..6964)
                     /gene="pth"
                     /locus_tag="PKX59_08645"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOZ90973.1"
                     /translation="MSKFLIAGLGNPGVEYAGTRHNIGFGVVDYIAQQHEYSFSSGRY
                     AHVCEWRHRGKPVFLIKPTTFMNLSGKAVQYWMQAEKIAIENILVITDDIALPFGTLR
                     LRAKGSDGGHNGLKSIQESLTNTNYARLRFGVGSNFAKGRQADYVLGNWDTEELAVIP
                     EKLKLAEEIVKSFIGHGVSRTMNDFNKR"
     gene            complement(7028..7663)
                     /locus_tag="PKX59_08650"
     CDS             complement(7028..7663)
                     /locus_tag="PKX59_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOZ90974.1"
                     /translation="MKSLTIKGTKRVGLTKQELKKLRADGKVPCVLYGGKDNVHFSTT
                     ALELRDLVYSPSVYTVNLDVNGEQHQAVMQDIQFHPVTDKIQHIDFLLLQNNKPVSIA
                     VPVTVKGSAEGVKQGGKLIQKMRKMQISALPANLPDAIEIDVTTLGIGQAVKVANIQR
                     DNITLLDSPNNIIVAVRTTRNVVEETPAAAAAAAPAAAKPAAAAPAAKPAK"
     gene            complement(7685..8620)
                     /locus_tag="PKX59_08655"
     CDS             complement(7685..8620)
                     /locus_tag="PKX59_08655"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019943843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOZ90975.1"
                     /translation="MAREVKLFSGEATRYLADNIALSYGLPLGDVIVSHFSDGEFSPY
                     FDETVRGCDVFIIQSTFAPTNNLFELLMLIDAAKRASAHYITAVIPYFGYARSDRKDK
                     PRVAIASKLVANLLTAAGVTRVMTMDLHAPQIQGFFDIPVDHLDASSIFVPYIKNLNL
                     PNLLIAAPDMGGVHRAREYAKFFNAEMAICDKHRKRANEVASMQVIGDVAGKDVILVD
                     DIVDTAGTLSKAAELLIEKGASSVRAFCTHAVLSGNAYETIENSKLTELIVTDTIPLR
                     KKSAKIVQLSTAELFSYAIRRVYEYSSISNLFVKS"
     gene            8730..8816
                     /locus_tag="PKX59_08660"
     tRNA            8730..8816
                     /locus_tag="PKX59_08660"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8764..8766,aa:Leu,seq:caa)
     gene            8861..9334
                     /locus_tag="PKX59_08665"
     CDS             8861..9334
                     /locus_tag="PKX59_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOZ90976.1"
                     /translation="MGSPGFDDIYMDLAKTLALKSHCVKMQVGAVLTKDTRIISLGYN
                     GPPAGTHNCDTEWPDDGCPRDSKGGCSLAIHAEQNAILYAAKNQVSVEGATLYVTLSP
                     CLACAKVVFTSGIKKVIFLNSYAQYKGIKSDEGVDFLRKFNVEVQQYTPGTVSGT"
BASE COUNT         2859 a         1686 c         1797 g         3022 t
ORIGIN      
        1 tatttgtaat aaataacctt cctcatcacg gtcaaccata attccccatt ttttcagttc
       61 ttccaaatct tcttcaataa ttcctacacg ttctttgacg gtgtcgtaat agcttcccgg
      121 cacatataaa aattctacac cacgtttgcg catttctgta atagtgtgaa caatatcatc
      181 tgttgctact gctatatgct gacagcctgc accttcataa aaatcaagat actcttctac
      241 ctgcgatttc tttttgccga tggcaggttc gttaatcgga aatttaatat aaccattgcc
      301 attggtcatt accttactca tcagagcagt gtattctgta gaaatatctt tgtcatcaaa
      361 tgttatgaga ttaaaaaatc ccattacttc agcataaaat ttactccaca cattcattcc
      421 tcctaactcc acattgccta ccatgtgatc aatatacttt aatcctgttg aaggtgggtt
      481 atagtccgat tgatactttt catatccggg aagaaaaatg cctttgtaat tttttcgctc
      541 gataaaaata tggattgtat cgccataggt atgaatacca gaccttctta cttcaccatc
      601 tttgtctttt agaacttcgg gtgacaaata tggtttagca ccacgtttca ccgtttgttc
      661 aaatgcatca gttgcatcat caacccaaag cgctattact ttaacaccat cgccatgttt
      721 tttgatgtga tcggcaatgg gattagaaga agttaaaggt gtagttagta ccaatcgaat
      781 ttttccttgt tgaagcacat aagatgttct gtcgcgcaca ccggtttcaa gtcctgcata
      841 ggcaagatcc tgaaaaccga aagctgtttt ataataatgc gcagcctgtt tagcattgcc
      901 tacataaaac tctacatagt ctgttcccaa cagtggaaga aaatcctgtg cctgttcaaa
      961 tatttttttg ttttctgaat agttgaaatt ttttacggta gttaaatcat ttgttgccat
     1021 tggttgtttg atttaaatac ccccgaagct ctgctctcct tggtggaggt gaaaaatgag
     1081 agagcaggag caggggtatg ttgcttcttt atattgtttc agactcgtgc atgcgccacg
     1141 actgataata ttcttctatt tcaatttctg ttgctgcctg tgtaattttt accggattaa
     1201 acggatcaat cattacagcc aactcattgg tttctttttt accaatactg cgttcaatag
     1261 ctcccggatg cgggccatgt gatagtccac ccggatgcag tgttatctgt ccttgtctga
     1321 tattgtttct actcataaaa tcaccatcaa cataataaag caactcatcg ctgtccatgt
     1381 tactgtgatg ataaggtgca ggaattgcct gcggatgata atcataaagg cgcggcacaa
     1441 aggagcacac tacaaagtta ctcgactgaa attgctgatg tataggcgga ggcatgtgta
     1501 tgcgtcctgt aatgggttca aagttaaaga ttgaaatacc atagggataa aaacatccgt
     1561 caaagcctac tgcatcgaac gggtgtgttt cataaatgta tggataaatc aatccgcgtt
     1621 ttttaatgtt tattagaaag tcacctcttt catcatgcgt ttcgagagaa tggggcaatt
     1681 taaaatcacg ttcgcagaat ggcgaatttt ccataaactg accatactga ttgcgataac
     1741 gtgcaggtgt ttcaatgggt gcatgcgatt caatgaacaa caatttattg tcggcagtgg
     1801 taaaatttat ctgatagatg ataccacgcg gaataataag gtagtcgcca tattcaaaat
     1861 ctatggttcc gaataatgtt tttaaagttc cgcttccacg gtgaacgaaa agcatttcat
     1921 cggcatcggc atttttataa aagtaatctg ttgtaccttt tgttggtgat gcaagcccta
     1981 tatgcatagc atcattcacg aataaaactt ttctgctctt cagataatcg ttttcgggtt
     2041 ttacttcaaa gcctaaaaaa cttcttgcct gcaagttatc ttcaactgca atttccggtc
     2101 gaacactata tggcttacca tgttccttaa ccatggtagg tgggttaaga tgataaacta
     2161 aggaagaaat accggagaat ccttctgtac caaaaagctc ttcatgataa aggcttccat
     2221 ctttcttacg aaatacaata tgtcttttat gcggtgtttt tccctgacgt tgataaattg
     2281 gcatgacaaa atatttttgc aaagaggtca aaaattcttg ttttttgata tgacatttat
     2341 cagtatgtag ggtgattttt gggatttggc ttggttgagt ggaaaaataa aagaaccaat
     2401 tattttaata ttttgctata tcttggtcat ggggttcgca tctgttagtt aattgttgtg
     2461 gcggctacaa ttaaattttg caactaatgt gaagaaactc ttttatttca tcccagtttt
     2521 ttgtgtaagg tgcaattccg gttatatcag gatcttcatt cgcatcttca aaataatgaa
     2581 tgctgcgata taaaaagtac aattgacttt tatcgtactt gtttacaaac cattgtagca
     2641 tttgctccca tccaaaattc tctaacaata catatacatc aaagaaatct ttctttttcc
     2701 ttcttccgca gatagtgtgc attttcatcg cagtaatgtc tgctatagaa aaatagctga
     2761 tagtatcttt gactaaaaat ggctgcaata atttagatgg ctcgtaaata aaatcacact
     2821 taatagaatt gatataggca aacaacatgt ttttgttgct gttgactcgt tcaaatatga
     2881 agcctgattc atttgtaagt acccgttcaa aaacgtaagt atcaaaaggt accgttgaaa
     2941 atatatccaa atcaatagaa tgcctgtgtt ttaattgcaa tgcaagcgat gtgccaccag
     3001 caagtgcaaa gtgttttttc acatcatcta cctcaaaaac tttttttaaa gtagcataag
     3061 ttgcttcatt aatgatagaa ggatgataca tggagctcgt gcaatggaat attaaaatac
     3121 caactacaga aatgagccgt tttagggtct aattctttgc ttttgcgtaa agcagcttgt
     3181 atttcatcta ttgaatatat tttttgaagg atataaaaat cattggtaaa acctcgtgtt
     3241 agtacacgct ctattataaa acgttgatga atttttaaat caatgtcttt tatagggtag
     3301 tcccaaaata attgttggct aaaaggaaag cgttcttctt ttatcttttt atctaatttg
     3361 gtcgtgtcaa tatatttgag aaagctattc attatggctc aaagatactc tctttaagct
     3421 taaactatga aatccaattt tatttctatt aaaacaaata gcatttaact taggatgaca
     3481 gtcctgctga attcatgtct tagttggtgt aagcttacga aacttttaaa atctttcaac
     3541 tggttatcct tagctgaata ataaattatt ttttcaaatt ctgaactgca gcttgataaa
     3601 attttaaaat aattttttaa agtgactgtt gtcatgttgc catcataata acaattaatt
     3661 tcttaatctt gtcaaggtat gaagaataga aatttgccta atcaggatta taaattaatt
     3721 acactaccca ataaaatgat taaagtgttg gtagatggaa aatttgcaca gttaaacact
     3781 gtgggcatgg aacaagtgtt tcatgcactg atgtttttat tagcctatcc tcagtcggag
     3841 aaacaagaag tgtttgcctt aaaaaagttg aacaccgtag ctgctatcat caaaaacaaa
     3901 gcggctgcat ttaaaaataa attaattaac agcggattgc ctttcactaa tatgtcagtg
     3961 cagtttacac atgattttct gcaatggtta ttgcagcaga aagagctaaa agtaaaattc
     4021 cggcagtttg taaattcaga aacagattta aatgagttgt tgaaactgac tttaaatccg
     4081 gcagaaaaat ctgaaacaac agcgggcctc agcaatgaag acttgcttca ctcgctcggt
     4141 attgaacaac acgaagtcct caactttcta ttagacgaat tttctaaaat ataccaacct
     4201 gaagtaaaag attatttgtt tgacagtgta ggtttgcaat gcgacttaat accgcaaaac
     4261 aaattttttt cattacccta taatcgcttc accaggaata aaactttcta tcacgaagaa
     4321 attttaaaaa agtttgatta taaaaatgtt ttagattctg aaattaatcc tgctgtaagt
     4381 ttatcagaga atgagcatgt tgaattgata aaaacaataa agtatgccat ggtattgact
     4441 aaccgagaga cggatacggt tacgtacatg gacgaaaaca cactgcagta ctttgagttg
     4501 gaaagaggaa tttctgtcgc aatttatggt atgacacctg acaggcaact tccttttgag
     4561 tcttatatcg gatacacttt atttaagaac ggatttccat gtgcctatgg aggcagttgg
     4621 gtgtttggta aacgggcatt gttcggcatc aatatttttg aatggtttcg tggtggcgaa
     4681 tcgggatatg tactttgtca gttgcttcgt gtgttccata aagtttttga tgttgactat
     4741 tttgaagtag aaccctatca gtatggatta gataatcctg atggcatttc ttcaggtgca
     4801 ttttggtttt attataagtt cggctttcgc cctgttgata aagaacttaa aaaaattgct
     4861 gagcgggagt ttcacaaaat aaaatccaag caaaactacc ggacacaaga aaaaactttg
     4921 ttgcgattta cagaaagtaa tatttcactt accttagaaa aaataaatca agcaaaaatt
     4981 gttgaggtta cttctgtaat ttcaggaatg ataaaaaaac aatttaaagg taatcgcatg
     5041 caagcagaac aggagtgtgt gagtttattt ttacaaaaag cacaaagggc tgtaaatcat
     5101 tcggagtata gtgaggcact acttgaaatg gcattgttgt gcgaagcgta taaaattaaa
     5161 gatgatgaaa ctatttcttt actgtatgct atggcacata taaagcctgt gaatccttat
     5221 gactatcagg gcatattgtt gaagtttttg gaaaaaattg cgaagtgatt tttattattc
     5281 cctaagcatt gaagtacata taaataaaaa agccattgtg ttaattacaa tggctttcta
     5341 tttttggtta aacaatatta tcgtctgata acaattcttt tattaatagc ctgaccattc
     5401 atgttaattt ttaaaacata aacgccatca ggcaattcag aaaggtttaa aatagttttg
     5461 ccgttgtttt gaataaagtt taaacgcttt acttctttcc caatcatatt gataacagca
     5521 attgaaattg cttttctttc gttgctgctt tcaaggttaa tgttgcctgt tgtaacggta
     5581 ggataaatgt ttaacacgcc ttgctctata ttgcttattc ccacaggccc gtttgaaata
     5641 gtaatatcat ctaaccctac attaaaacca agtgaatctt ttatgtattc aaattttacc
     5701 tgaatatctg ttgtgttcag acttagtgtc taaaatgctc ccgtctgtga aacaggattg
     5761 taggtttgaa tattgttcca gtttgtgcca tcagcggtag tgtaaatttt aaaaggattg
     5821 ggctgttgtg tattgccatt acctttcata taaaatttta agtgggtagc attactgaat
     5881 gatggtgaaa taatagtagc tcctgttaca ttaaatttat acgaattgca ggagatgcca
     5941 caataagtag gtgtgttata aaaagtttta ccgacagccg ttgtgtcgtt gttggtgata
     6001 gtccaaccgg cagggatgct tgttactaat ccgtcatatc cggtaaaatc gtacgatacc
     6061 aatacctgtg aaaatacatt ggtggtaagt acagcaaatg caattgtgag taggcgttta
     6121 atcatgttct taaattattt atgcaaagat aagcattgaa aatgaataaa atggaattta
     6181 aatgaataaa tcattcagta atgttacgtt aaagtagcaa gattttaatt cagaataatt
     6241 ttggtttttg ccgataatgc taaatggtat ttgtccattt ataaaagtgt ttattgaatt
     6301 ttgattggaa taattttaag aaacaaatgt attgtattaa gattgaaaac aatagtttac
     6361 tgcaaaaatg tatttggaat acgataagaa acttctgtta tcgcttatta aaatcgttca
     6421 ttgtgcggct tacaccatga ccgataaaac ttttaacaat ctcttctgca agttttaatt
     6481 tttcaggaat gacagcaagt tcttcagtgt cccaatttcc taaaacataa tcggcctgac
     6541 gacctttggc aaagttgctt ccaacaccaa aacgcagacg ggcatagttt gtgtttgtca
     6601 gagattcctg aatacttttt agtccgttgt gaccgccatc gcttcctttg gcacgcaggc
     6661 gcagtgttcc aaaaggcaag gcaatatcat ctgtgatgac aagaatattt tcaattgcaa
     6721 ttttttcggc ctgcatccaa tactgaacag ctttaccact aaggttcata aaggtggtag
     6781 gctttatcag aaaaacaggt ttgccgcgat gccgccattc gcaaacatga gcatagcgtc
     6841 cgctgctgaa actgtattca tgttgctgag cgatataatc aacaacacca aagccaatat
     6901 tgtgccttgt gccggcatat tcaactccgg gattaccgag acctgcaatt aaaaatttag
     6961 acattattta ttttaaacaa agaagccgga tattggaata cccggcttca atagattgac
     7021 aaaaaaatta tttagccggt ttagcagcag gtgcagcagc agccggttta gcggcagcag
     7081 gtgcagcagc ggcagccgca gcaggagttt cttcaaccac atttcttgtt gttcttactg
     7141 caacaataat gttgtttgga gaatcaagta atgtaatgtt gtcgcgctga atattagcaa
     7201 ctttaactgc ctgaccaatg cctaatgttg taacatcaat ttcaatagca tcaggtagat
     7261 ttgcaggcaa tgcactgatt tgcattttgc gcattttctg aatcaatttt ccaccttgtt
     7321 taaccccttc tgcacttcct ttaactgtaa caggcactgc tatagaaaca ggcttgttgt
     7381 tttgaagtaa taagaagtca atgtgctgaa ttttgtcagt tacgggatga aactgaatat
     7441 cctgcataac agcctgatgt tgttctccat ttacatcgag attaaccgtg taaacgcttg
     7501 gagaataaac caagtctctt agttcaagtg ccgttgtgct gaagtgcaca ttgtctttcc
     7561 caccgtataa cacgcatggt actttaccat cggcacggag ttttttcaac tcttgtttgg
     7621 taaggcctac tcttttggta cctttaatcg ttaatgattt cattgtaata tataatttat
     7681 aaatttaaga tttaacaaac agattgctta ttgaagagta ttcatacact cttcttatag
     7741 cataagagaa tagctctgct gttgagagct gaactatttt agctgacttt ttacgcaatg
     7801 gaatagtatc ggtaacaatt aactcagtaa gttttgagtt ttcaatcgtt tcgtaagcat
     7861 tgcccgacag tacagcatgt gtgcagaatg cacgcacaga gctggcacct ttttctatta
     7921 aaagttcggc agcttttgat aatgttccgg cagtgtcaac aatgtcgtca actaagatta
     7981 catcttttcc ggcaacatca ccaataacct gcatgcttgc cacttcgttg gcgcgtttgc
     8041 ggtgtttgtc gcagatggcc atttctgcat taaagaattt tgcatactcg cgggcacgat
     8101 gaacaccgcc catgtccggt gctgcaatga gcaggttggg caggtttaag tttttgatat
     8161 aaggaacaaa gattgaagag gcatctaagt ggtcaaccgg tatgtcaaaa aatccctgaa
     8221 tctgaggtgc atgcaagtcc attgtcatca cacgggtgac accggcagca gtgagcagat
     8281 tggctactaa tttactggca atggctactc tgggtttgtc tttgcggtcg ctgcgggcat
     8341 aaccaaaata aggaattacg gcagtgatgt agtgtgcaga agcacgcttg gcagcatcaa
     8401 taagcatgag caattcaaac aaattgttgg tgggggcaaa ggttgactga atgataaata
     8461 catcacaacc acgcactgtt tcgtcaaaat agggagaaaa ctcgccatcg ctgaaatgcg
     8521 aaacaatgac atcgccaagg ggtaaaccat aactaagtgc aatgttatca gccagatagc
     8581 gtgtagcttc accggaaaac aatttgactt ctcttgccat agataagggg ggattctgaa
     8641 agggctgcaa agaaaggaaa aatttcgctc aggtcaaatg gattgtgtaa aattaatact
     8701 tttgcagcct cgttttttga atagaattta cccggatggc ggaattggta gacgcgctgg
     8761 tctcaaacac cagtgagcta aacctcatgc cggttcgatc ccggctccgg gtaccagcaa
     8821 gccctcacac tgagggctta gctttttaat ataatcagct atgggttcac ccggttttga
     8881 cgatatttat atggatctgg caaagacact tgccttaaaa tcgcactgtg ttaaaatgca
     8941 ggtgggagcg gtgctcacca aagatacgcg catcatctct cttggatata atggtccgcc
     9001 tgcgggcaca cacaattgcg atacggagtg gcctgatgat ggttgcccgc gcgacagcaa
     9061 gggtggttgc tccttagcca ttcatgccga acaaaatgca attctttatg ctgcaaaaaa
     9121 tcaggtttcg gtagaagggg ctactctcta tgtaacactt tcgccatgcc ttgcctgtgc
     9181 taaggtggtg tttacttcag gaataaaaaa agtgattttc ttaaattcct atgcgcagta
     9241 taaaggcata aaatctgatg aaggagttga ttttttaaga aaatttaatg tagaagtgca
     9301 acaatatact cccggaactg tttccggtac ataatatctt ttcatgcagt ctgatcagaa
     9361 aaaa
//
LOCUS       DAOOOI010000074         9361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1444_length_9361_cov_9.412744, whole genome shotgun sequence.
ACCESSION   DAOOOI010000074 DAOOOI010000000
VERSION     DAOOOI010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1444_length_9361_cov_9.412744"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PKX59_08670"
     CDS             complement(<1..1076)
                     /locus_tag="PKX59_08670"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOZ90977.1"
                     /translation="MEKATQYSEQNKDRFLNELLELLRIPSVSADSKYKADLVRTAEF
                     VKSSLEKAGADKVEICPTGGHPIVYGEKIIDPKLPTVLVYGHYDVQPPDPLDLWHSPP
                     FEPVIKKTDLHPEGAIFARGSCDDKGQMYMHVKAFESMMKTATLPCNVKFMIEGEEEV
                     GSSNLGIFVKANKERLKADVVLISDTSMIANDTPSIDVGLRGLAYMEVEVTGPNRDLH
                     SGVYGGAVANPATILCQMIASMHDENNHITVKGFYDDVQKVSDADRADLAKIPFDEKE
                     YMKDLGIEQVWGEKGFTSIERTSIRPTLEVNGIWGGYTGEGAKTVLPSKAFAKISMRL
                     VPNQNSETISKLFEKHFLAIAPPYV"
     gene            complement(1103..1987)
                     /gene="fabD"
                     /locus_tag="PKX59_08675"
     CDS             complement(1103..1987)
                     /gene="fabD"
                     /locus_tag="PKX59_08675"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOZ90978.1"
                     /translation="MSSKRAYIFPGQGSQFSGMGKDLFEQSDAARSIFEKANSITGFS
                     ITDVMFSGTDEELKQTKVTQPAIFVHSCAIIASLGDNFHPDMVAGHSLGEFSALVANK
                     CLSFEDGLKLVMERALAMQAACELKPSTMAAILGLDDEKVESICAGINDVVVCANYNC
                     PGQLVISGTIDGVKTACEQLTAAGARRALLLPVGGAFHSPLMEPARERLANAIANTTF
                     AVPICPVYQNVNALPSTDPQQIKQNLIAQLTAPVRWTQTVIKMANDGATDFIEAGPGK
                     VLQGLVKKIHKEAQVMSA"
     gene            complement(2074..2484)
                     /locus_tag="PKX59_08680"
     CDS             complement(2074..2484)
                     /locus_tag="PKX59_08680"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOZ90979.1"
                     /translation="MIEFEHKVRVRYAEADRMGYVYYGNYAVYFEVARVEMLRSLGLP
                     YKLLEDKGIILPVSEFSIQYKKPAFYDDVLTIKTVIRKRPSVRIYFDYETFNEKGELL
                     NTASVTLVFVSKETGKPINPTEEFNKVFTPYFQN"
     gene            2711..4990
                     /locus_tag="PKX59_08685"
     CDS             2711..4990
                     /locus_tag="PKX59_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOZ90980.1"
                     /translation="MTSVADTIHVEKYIISIDTIDFTGKQIKAKAQLQIVARQNNLST
                     IELDLFQLNVDSVYETSGNLSFTYQNDKLNISLPFVLNTNDTANLTVVYHGSPQKDAS
                     GFGGFYFFPNMAFNLGVGFTADPHNLGKVWFPCLDVFDDKSKYEFYITTPQTQKAYCN
                     GFLNDSIINANTITWHWIMNDYIPTYLACMATGPYTSWERTINGIPVEIAALPADTAA
                     VSATFVNLPAAINLFTQYYGNYPFNKIGYTLVNFTGGAMEHATNIAIGKAFINGTLGY
                     ETLWAHELSHMWWGDKVTCKTAEDMWLNEGWASFNEALFTEALYGITAYKDWIRTNHR
                     KVLQLAHINDGGYLALNNVPTSVTYGNTAYQKGADIVHTLRNYAGDSLFKVGAQHYLD
                     TFAYSNASSVDMEQAFTSSTGTDYSRFFTDWVYTPGFPHFSIDSVQYVPGGLDHYFVY
                     TRQRNKGSNGHIYEMPVDITFSDGITDTTVTILIDSATQMFHIPLIMANATWIALDRG
                     EKISDAISDYEKVITATGSQVMKETSSTLNVQSIGATPSTVRIEHHWVQPDGFKGSNP
                     GIRLSDYHYWQAEGIFSPGFVSKISFQYDGSTNGLTGYIDNTLITGREDSIVLLYRQN
                     TADDWHLLSTTKSMGSPLDKRGSITLDTLKIGEYVLGYYDVSVNTSSLQQNSDKFMKI
                     TPNPASHEFTIEVNLEQGDSGIVNIMTLSGKTIKTISVNHEQKKYRIDSGKLKSGEYI
                     LHLKSANGLISSNKILIQK"
     gene            5069..7369
                     /locus_tag="PKX59_08690"
     CDS             5069..7369
                     /locus_tag="PKX59_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ90981.1"
                     /translation="MRKALLTLIAAAGSMFAQAQWISQNVPMTYDGYMFDMETVDANT
                     CWGGLWNGTATSPYTLDFARTIDGGNTWTTGTIPATTGWVISNIWPLSADTCFVMMCN
                     INAGGGMIYKTVDGGTTWTQNSIAGMYSQATSFGNVIYFSDSQNGFTMGDPVGTGVNK
                     RYELYTTNDGGDNWTAVPIANIPALTNAGEYGITNLFSAVGSNIWFATTYGDVLHSSD
                     NGLTWTKSASGFPPYTANGNRQDITDIAFSDANNGMIVQVNASAYIVKQTTDGGATWT
                     TVTTLGNFYPTEIEAVPGTSIYVSGGSNTTYGFGSSFSTDNGLTWTPLDTNFSHTTFD
                     FIDASTGYGAEYIAAGSPGGAWKFSGTLSNNPLNDDCAGATNINAQFGQAPGTLTSSG
                     PYDNTNATTGPQDPTTGFACFGEPNGSGTAPTLDNTLWFTFTGDGNTYFIEAPNTCAG
                     VTDPMDEGDTQIAIYTGTCGNLTPVACNEDGPNANATNSYYPAGLNFTTTPGTVYYMI
                     VDGFNNVGTISNGQFCVQVTNQSVIACGDTTISSGLAVLNKPVICYDSTLTIAVSGIK
                     APTVGTTKGFSIMVSNADISGNNNPMSTGAVVGGTGTIAVGSSGTFQTNLVNNASNPF
                     GAGVYYITPIVYGNATGTGNITALTLDPNCTYTGTSVMVEFLAQGVICPTGIKELNGA
                     AFAVTGIYPVPVKNILNIAVESAKSTTMSISIKDVLGRTVTSSTQSVNNGSNTISIDT
                     QNLSSGVYTLTVNNEKQQSTVKFVKE"
     gene            complement(7531..8622)
                     /locus_tag="PKX59_08695"
     CDS             complement(7531..8622)
                     /locus_tag="PKX59_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOZ90982.1"
                     /translation="MEKEQIRNSFQHVNYRFEEGEHGGHKVIWIHFPYNRKLIDDLKT
                     YVKAKWSFGKKCWYVQDNTYFRRLLHLENSFIGNAALQNLSRSNQDAMKRFIEQLKLK
                     GYSPNTIRTYSQEFYQLLKVLKGNSVNSLTPERLRSYFLYCIRTLKVSENLIHSRTNA
                     VKFYFEQVLHRKKMFFEIPRPKRPSTLPKSINQKDLKKMFSTLDNLKHQLLLKLCYGM
                     GLRVSELVNLKILDIDSGSMQVLVSRGKGKKDRYVTLPKSILADLRLYYKEYKPKVFL
                     FEGMHGGQYNIRSVQQVFKDALKNAKVNKKVGVHGLRHSYATHLIEQGTDIRFVQELL
                     GHSNIKTTMAYTKLTDQIRRKIKSPLDNF"
     gene            complement(8811..9122)
                     /locus_tag="PKX59_08700"
     CDS             complement(8811..9122)
                     /locus_tag="PKX59_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOZ90983.1"
                     /translation="MTTKKEIIDNETCPAQGLLKSLSGKWKPEIFRLAVEAPLRFSSL
                     LRQIEGSNKQTLSVALRELEEVGLLEKVVIQQKPLHIEYNLTEKGKSLIPVFKQLESL
                     G"
BASE COUNT         2953 a         1838 c         1634 g         2936 t
ORIGIN      
        1 acataaggtg gtgcaatagc aagaaaatgt ttttcgaata gtttagaaat tgtttcagag
       61 ttttggtttg gtaccagtcg cattgaaatt tttgcaaatg cttttgatgg caaaacagtc
      121 ttagctcctt caccggtgta accgccccaa attccattca cttcaagagt aggtctgatg
      181 cttgttcttt caatagacgt aaaacctttt tcaccccaca cctgctctat gcctaaatct
      241 ttcatgtatt ctttttcgtc aaatggaatt tttgccaaat cggctctgtc ggcatcgctc
      301 actttctgaa catcatcata aaatccttta accgtgatat gattgttttc atcatgcatt
      361 gaagcaatca tctgacaaag tatcgttgca ggatttgcaa ctgcaccacc ataaacacca
      421 ctatgcagat cgcggttagg tcctgtaact tcaacttcca tatatgccaa tccgcgcaag
      481 ccaacatcaa tagatggtgt atcgtttgca atcattgaag tgtcggaaat caatacaaca
      541 tcagccttta gacgttcttt atttgcttta acaaatatgc caaggtttga tgaaccaact
      601 tcttcttcac cttcaatcat aaacttaaca ttgcatggaa gcgtagcagt tttcatcata
      661 ctttcaaatg cttttacatg catgtacatc tgacccttgt catcgcaaga tccacgtgca
      721 aagatggctc cttcgggatg caaatctgtc tttttaatta ccggttcaaa cggtggtgaa
      781 tgccataaat ccaatggatc gggtggctga acatcataat gtccataaac taaaacagtt
      841 gggagttttg gatcaataat tttttcgcca taaacaatag gatgcccgcc tgtaggacaa
      901 atctctactt tatcggcacc tgctttttca agacttgatt ttacgaactc tgctgttctt
      961 accaaatctg ctttatactt tgagtctgca cttactgaag gtatgcgcag caattcgagc
     1021 agttcgttta aaaaacggtc tttattctgt tctgaatatt gtgttgcttt ttccatttaa
     1081 actttttatt aatgattata aattatgcac tcattacctg agcttcttta tgaattttct
     1141 ttacgagtcc ctgcaaaact tttccgggtc cggcttctat aaaatcagta gcgccatcat
     1201 ttgccatttt aataactgtc tgtgtccatc gtacaggagc agtaagttgg gcaataagat
     1261 tttgtttgat ttgttgcggg tcggttgaag gcaatgcatt tacattctga tatacagggc
     1321 aaattggtac tgcaaatgtt gtattagcaa ttgcattggc taatctttcg cgtgcaggct
     1381 ccatgagagg tgaatgaaac gctcctccta ccggaagcag tagtgcacgt cttgcaccgg
     1441 cagcagtaag ctgttcacat gcagttttta ctccgtcaat agttccggaa atgactaatt
     1501 gtcccgggca attatagtta gcacaaacaa caacatcatt tattcctgca caaattgatt
     1561 ctaccttttc gtcatcaagg ccaagaattg ctgccatagt tgaaggtttc aattcgcatg
     1621 ctgcctgcat ggcaagagca cgttccatca ccaattttaa tccatcttca aaactcaaac
     1681 acttgttggc taccaatgca gaaaattctc ctaatgaatg tcctgcaacc atgtcgggat
     1741 gaaaattgtc gccaagcgat gctatgattg cacatgaatg aacaaaaatt gcaggctgtg
     1801 taacttttgt ttgtttcagt tcttcatcgg ttcctgaaaa cattacatca gtaattgaaa
     1861 agccggtaat gctgtttgct ttttcaaaaa tactgcgagc agcatcagac tgctcaaaca
     1921 aatcttttcc cattcccgaa aattgtgaac cttgtccggg aaaaatatat gcacgttttg
     1981 aactcataaa atattagtca gttaattcag atgttttgat taaaggtgaa aatcttttgc
     2041 caaaactaag taaaacaaaa taaaacaagc tgcctaattc tgaaaatatg gtgtaaacac
     2101 tttgttaaat tcttctgtgg ggtttatagg cttgcccgtt tctttactga caaagactag
     2161 tgtgaccgaa gctgtattga gcaactcccc tttttcatta aatgtttcat aatcaaaata
     2221 tattcttact gatggtcttt tcctgataac agtttttatt gtcagaacgt catcgtaaaa
     2281 tgccggtttt ttatattgaa ttgaaaattc tgagacaggt aatataattc ccttgtcttc
     2341 aagtaatttg tagggcaacc caaggcttcg caacatctct acccttgcta cttcaaagta
     2401 aacagcatag tttccataat aaacataacc cattctgtcg gcctctgcat atctgactct
     2461 tactttatgt tcaaactcaa tcattacttt tcgaaaatat taaatgcaaa ttaaaagaaa
     2521 atgctgcaat gaatatttaa attaaattta ttaggacata aaattctatt tttgaaagtt
     2581 caatatatca acttaacttt ttatgcattc atttcaattt aactttaaat tacaattgca
     2641 tcaaacattg ataaaaaaac ttttaagtct tgtgttattt ttagtttcat gcaacattag
     2701 cttatctgcc atgacatctg tagcagatac tatccatgtt gagaaataca ttatcagtat
     2761 tgacacaata gactttacag gcaaacagat taaagcaaaa gcacaattgc aaattgttgc
     2821 aaggcaaaat aacctgagta caatcgaatt ggatttattt caattaaatg ttgattctgt
     2881 ttatgaaaca tcgggcaacc tatcctttac ttatcaaaat gataaattaa acatcagtct
     2941 gccttttgtt ttaaacacca atgacactgc taatcttacg gtagtttatc atggtagtcc
     3001 gcaaaaagat gcctcaggat ttggtggatt ttattttttt ccgaatatgg catttaacct
     3061 tggagttggt ttcactgccg acccgcacaa ccttggtaaa gtttggtttc cttgccttga
     3121 tgtttttgat gataaaagta aatatgaatt ttacatcact actccacaaa cacaaaaagc
     3181 atattgcaat ggttttttaa atgattcaat aattaacgca aataccataa cgtggcattg
     3241 gataatgaac gattatattc caacatattt agcttgtatg gcaacaggcc cttacacttc
     3301 atgggagaga acaatcaatg gcattcctgt tgaaattgca gcattgccgg ccgacactgc
     3361 ggcagtgtca gctacgtttg ttaacctgcc ggcagcaatt aatctgttca cacagtatta
     3421 tggaaattat ccttttaata aaataggata tacgctcgtc aattttaccg gtggtgcaat
     3481 ggaacatgcc accaacattg caattggtaa ggcatttatt aatggaacac ttggctatga
     3541 aactttgtgg gcacatgaac tgtcacacat gtggtggggc gacaaagtga cttgtaaaac
     3601 tgcagaagac atgtggctta atgaaggctg ggcatcgttt aatgaagcat tgtttacaga
     3661 agcgctttat ggtataacag catataaaga ctggattcga accaatcaca gaaaggtatt
     3721 acaacttgca catatcaatg atggtggcta ccttgcactg aacaatgttc cgacttctgt
     3781 tacctatgga aatacagcat atcaaaaagg tgccgatatt gttcataccc ttagaaatta
     3841 tgcaggtgat tcattattta aagttggtgc tcaacattat ttagacactt ttgcttacag
     3901 caatgcttcg agcgttgaca tggaacaggc atttacttct tctacaggaa cagactactc
     3961 acgttttttt acagactggg tttacactcc cggcttccca catttttcta ttgactctgt
     4021 tcaatatgtt cccggaggat tagatcatta cttcgtttat acacgtcagc gcaacaaagg
     4081 tagcaacgga catatatatg aaatgcctgt tgacattact ttcagtgatg gaataacaga
     4141 caccactgtg accatattaa ttgactctgc aacacagatg tttcatattc ctttaatcat
     4201 ggctaatgca acgtggatag cgttagacag aggcgaaaaa ataagcgatg ccatttctga
     4261 ttatgagaaa gtaattacag ccacaggttc gcaggtaatg aaagaaacaa gctccacttt
     4321 aaatgtacaa tcaattggag caacaccgtc aacagtcaga attgaacatc attgggttca
     4381 gcccgatggt tttaaaggca gcaatcccgg catacgatta agcgattatc attactggca
     4441 ggcagaggga attttcagtc ccggctttgt gagtaaaatt tcattccaat atgatggatc
     4501 aaccaatggg ctaaccggat atattgacaa cacactgatt acaggacgcg aagacagcat
     4561 tgtacttctt tacagacaaa acactgctga tgactggcat cttttatcta ctacaaaaag
     4621 tatgggttct ccattagaca agagaggttc tataacccta gacaccttaa aaataggcga
     4681 atatgtactt ggctactatg atgtttctgt aaacacatcg tctcttcaac agaattcaga
     4741 taaatttatg aagataaccc cgaatccggc atcacatgaa tttaccattg aagtaaattt
     4801 agaacaagga gatagtggca tagttaacat tatgacttta agcggaaaaa cgattaaaac
     4861 catttctgta aatcatgaac aaaaaaaata cagaattgac agtggaaaat taaaaagcgg
     4921 agaatatatt cttcatttaa aaagtgccaa cgggctgata agcagtaaca aaattttaat
     4981 ccagaaataa atcaaaaaac ctataaatta taaacgaatg tttcattaca tttgccactt
     5041 aatcatataa aaataaaatc aaataatcat gagaaaagct ttactcactt taattgcagc
     5101 cgcaggcagc atgtttgcac aggcgcaatg gattagtcaa aatgttccca tgacgtatga
     5161 cggttacatg ttcgatatgg aaactgtaga tgccaatacg tgctggggtg gattgtggaa
     5221 tggtacagcc accagtccat acactttaga ttttgccagg actattgatg gcggaaacac
     5281 ctggactaca ggaactattc ctgcaaccac aggatgggtt atctccaaca tctggccatt
     5341 aagtgccgac acttgctttg tgatgatgtg caacatcaat gcaggcggtg gaatgattta
     5401 caaaactgtt gacggtggta ccacatggac acaaaattca attgccggta tgtattctca
     5461 ggcaacatct tttggaaacg taatttattt ttcggattca cagaatggat ttactatggg
     5521 cgaccctgtt ggtacaggcg ttaacaaacg ttacgaactt tatactacca acgatggtgg
     5581 tgataactgg acagcagtgc ctatagccaa cattcctgca ttaactaatg cgggtgaata
     5641 tggtatcacc aacttgtttt cagcagttgg aagcaacatt tggtttgcaa ctacctatgg
     5701 tgatgtactc cattcatcag acaatggttt aacatggaca aaatctgctt caggctttcc
     5761 tccttataca gcaaacggta atcgtcagga tattacagac attgccttca gcgatgctaa
     5821 caacggaatg attgttcagg ttaatgcatc tgcatacatt gtaaaacaaa caactgatgg
     5881 tggtgctaca tggactactg taacaacttt aggtaatttc tacccaacag aaattgaagc
     5941 cgttcccgga acatcaatct atgttagtgg tggttctaat actacttatg gctttggttc
     6001 atctttcagt acagacaatg gcttgacatg gacaccactt gacacaaact tctcacatac
     6061 aacgttcgac tttattgatg catcaacagg ttacggtgca gaatatattg cagcaggtag
     6121 cccgggtgga gcatggaagt tttcaggaac tcttagcaac aaccctttaa atgatgattg
     6181 tgcaggtgca acaaacatca atgcgcaatt cggtcaggct cccggaacat taacttcatc
     6241 aggaccttat gataacacta atgctactac aggtccacag gatccaacaa caggctttgc
     6301 ttgttttgga gagcctaacg gaagtggtac tgcacctact ttagataaca cactatggtt
     6361 tacattcacc ggtgatggaa atacctattt tattgaagca ccaaatactt gtgcaggtgt
     6421 aaccgatcca atggacgaag gcgacacaca aattgcaata tatacaggta catgtggcaa
     6481 ccttactcct gttgcatgta atgaagatgg tccaaatgca aatgctacaa actcatatta
     6541 tcctgcaggt ttaaacttta ctacaacacc gggtacagtt tattatatga ttgttgacgg
     6601 ttttaacaat gtaggaacaa tatcgaacgg acaattctgt gttcaggtaa ccaatcaatc
     6661 agtaattgct tgtggtgata caacaatttc ttcaggttta gctgttttaa ataagcctgt
     6721 aatttgctac gacagtactt taacaattgc tgtttcagga attaaagctc ctacagttgg
     6781 aacaactaaa ggattcagca taatggtttc aaatgctgac atcagtggta ataataaccc
     6841 aatgtcaaca ggtgctgttg ttggtggaac tggaacaatt gctgttggta gcagtggtac
     6901 attccaaaca aacttagtta acaacgctag caatcctttt ggtgcaggtg tttactacat
     6961 cactccaata gtttatggta atgcaacagg aacgggaaat attacagcgc ttactcttga
     7021 ccctaactgt acctatacag gtacttccgt tatggttgaa ttcttagcac agggagttat
     7081 ctgcccaaca ggcataaaag aacttaatgg tgctgctttt gcagttacag gaatttatcc
     7141 tgtacctgta aagaacatat tgaacatagc tgttgaatct gcaaagtcaa caacgatgtc
     7201 aatttcaatt aaagatgtac ttggaagaac tgtaacttca agtacacaat ctgtaaacaa
     7261 tggcagcaac accatttcta tagatacaca aaacctttca tcaggtgtat atacacttac
     7321 agtaaacaat gaaaaacaac aatcaactgt taagtttgta aaagaataat tcagattgtt
     7381 tgaaactaca aaaggctgcc tcgtaatggc agcctttttt tatgggtaac ttatactaca
     7441 cattattcca cattaaatta tgagccttaa gttttgaata accatgtttt gatatatcct
     7501 gaaacaaata aatcttcaat agttacgcag ttaaaaatta tccaaagggc tttttatttt
     7561 tcgcctgatt tgatctgtca gtttggtata agccatcgta gttttaatat tactatgacc
     7621 taaaagttcc tgtacaaacc gtatatctgt tccctgctca atcaaatgtg tagcatagga
     7681 atgacgaaga ccgtgcactc ctactttttt attgacctta gcatttttta atgcatcttt
     7741 aaatacttgt tgtacacttc ttatattata ctgaccgcca tgcattcctt caaacaaaaa
     7801 aacttttggc ttatattctt tatagtacaa tcttaaatct gcaaggatag attttggcaa
     7861 agtaacatat ctgtctttct tacctttgcc cctactcact aaaacctgca tactgccact
     7921 atcaatatcc agaatcttaa ggtttaccaa ttcacttaca cgcaacccca taccgtaaca
     7981 caattttagt agtaactgat gttttaagtt atccaatgta ctaaacattt ttttcagatc
     8041 cttttgatta atagatttag gcaaggtgct gggtcttttg ggtcttggaa tttcaaagaa
     8101 catcttcttt ctatgtagca cttgttcaaa ataaaactta actgcatttg tcctactatg
     8161 aattagattt tctgaaacct ttaaagtgcg gatacaatac aagaaataag acctcaagcg
     8221 ttcgggagtc aggctgttta cagaattgcc tttaagcacc ttcaataatt gataaaattc
     8281 ctgcgaatag gttcgtatgg tatttggact ataacccttc aactttaatt gctcaataaa
     8341 tcttttcatt gcatcctggt tgctgcgaga aagattctgt aaagcggcat ttccaataaa
     8401 actattttct aaatgtaaga ggcgcctgaa atatgtatta tcctgtacgt accaacactt
     8461 tttaccaaaa ctccattttg ctttaacata agttttcaaa tcatcaatca gttttcggtt
     8521 gtaagggaaa tgaatccaaa taaccttgtg acctccatgc tcgccctctt caaaacgata
     8581 attaacatgc tgaaaagaat ttctgatttg ttccttttcc atcttcttcg tattttgagt
     8641 agtttatatc attcaaaagt atatattttt ttatcattca atacaaaata cgatttcgta
     8701 ctatttttat ataacattga cttactgtaa tatagcaatc tttttaccgg cttcttttat
     8761 agcgttagga acttcgtata tttgtgagtt agcggcaagt ataaccgaca ctacccaaga
     8821 ctttctaact gcttaaagac aggaattaac gacttccctt tttcggtaag attatattct
     8881 atatgcaaag gcttttgctg aatgacaact ttttctaaca gcccgacttc ttctaactct
     8941 cttaaagcga cagacaaagt ttgtttgtta gaaccttcaa tttgacgcaa caaactacta
     9001 aaacgcaaag gtgcttcaac agccaaacga aaaatttcag gtttccattt gccagacagc
     9061 gacttcaaaa gcccttgtgc aggacaagtt tcgttatcaa taatttcttt tttggtagtc
     9121 ataattttct gacttattgc gtagttgaat ataagtcctc aactttgcac aaagataaac
     9181 aaatggtgta agacacttta caaaaaatga aattaactta tcttatttta atcacaacat
     9241 tcacaatcgg caatacaagt tgtcattctc aaaacaaaaa aaatatgcaa gagcaaaaca
     9301 atcctttaat gtgcgaccca gaaagtggta tttgcgaaat acctactaac aaaataagtt
     9361 c
//
LOCUS       DAOOOI010000075         9205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1475_length_9205_cov_99.047322, whole genome shotgun sequence.
ACCESSION   DAOOOI010000075 DAOOOI010000000
VERSION     DAOOOI010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9205
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1475_length_9205_cov_99.047322"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..503
                     /locus_tag="PKX59_08705"
     CDS             192..503
                     /locus_tag="PKX59_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOZ90984.1"
                     /translation="MDSTTEKTLQDLGRKVKMLREAKDISQDELASKLNLVNGHSYIS
                     KIEKGAVNITIEKMCEVLSVLDCEFTVFVKPNFKLSQNKAKKTNEKKGVLKPKKISFT
                     S"
     gene            525..2120
                     /locus_tag="PKX59_08710"
     CDS             525..2120
                     /locus_tag="PKX59_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90985.1"
                     /translation="MNGIVEDICYAVLIYNDFFIILRDKVKLLMSKKGKITMFNNTNG
                     LLLAPKFIELDSGKKQSFSWDNEDYRQSVVNPDISNAIAKKEDFLAFNFRHISATIVG
                     AWSWKATEFNEKVLKEAKSLLEYKPALVNHNAETGNIIGVNGKVVYENAYKANDGTII
                     PAGLNAPIWIDGKLHTDICRKLSAFPVPHIQSVSVSLLFEWEPSHTFTDQSGNEDDWM
                     FEMRIGTLVDGTMVRRVVTKIVDFYETSLVFLGADPFAKIVDTEGKLVNVEKSAIVGR
                     QMFSDDKIGSDYYKRSNGHYYLMDDVTFANEKSINLRERINVDFSKNGNSPKNNEMKV
                     LKLSGKKEDYAQEVLTTLEESFKVEFVELKDETETITNLTKEVEEFKSAKTTAEESLA
                     KVQSELTEAKNAFAEYEKIVPVAELSEFAKVIDLAQVKPLAEFGRKVELSKREECIRL
                     YKVSVKDKTNDAVIKTINEASFDALEGLMQQYGGTCMEQFGGKCDECNSTKVSFRSSV
                     NEGEQKEETEKTSFVSMAEQIRN"
     gene            2156..2599
                     /locus_tag="PKX59_08715"
     CDS             2156..2599
                     /locus_tag="PKX59_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90986.1"
                     /translation="MTGSIQTTPTQVIKNMTSDALQLTFVSSAGSEIKAGQEVYQHTD
                     GTVKKRTLGTQIPLGIALSDTPDGERVLVRVPHQMVIEGIAKGGALNAGVAVVPDGTL
                     NADGYPNYVAAGASAYVSAFTLKAAVEDARVRIGIIQGVYLTPGA"
     gene            2642..3988
                     /locus_tag="PKX59_08720"
     CDS             2642..3988
                     /locus_tag="PKX59_08720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HOZ90987.1"
                     /translation="MKENLLKLRKHNLAVFKGDKTDRLQADEVKTLTEETIPDFAKEI
                     ALTRQGRATDAYGRVIPAYNISFADAISIHYGIQPAKQTESGEKLSYKQQQKHVVKEF
                     LKQMDIFMGSDTLKTAAARLGNANLGVQSLTDALTKYSQFDALANTSGVPADYRFIIP
                     ELILTAIRLDYDASSLHQGWVATTQSISQDEITMPFIKKGNAVARKVGEGESIEFGSV
                     SFGKKKAGVFKVGLGFKITDELIERSSLDMIFTFLGEVGVDMSICADYEAYRVLVNGE
                     QSSGAESAPTIGVATANTFNFKDIKRGTSRLRRLKRMPDTIITGENDGIDITSLPEFQ
                     GFNGGNKMATIQSILGVPDTLKNDVYTVGSNQIMLLDSTKAMAKLQYGSMKMEERRNP
                     QTQENEIFVSDYIGFSIIRRDARLIIDKSVLFSAQGFPAYMDIDARINEAFKYHEE"
     gene            4014..4463
                     /locus_tag="PKX59_08725"
     CDS             4014..4463
                     /locus_tag="PKX59_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90988.1"
                     /translation="MEIFVKLKDASNSHSILHQQKNLFGKKVEKVLKDVYVSKLLKDG
                     VIVEAKETEYDAWVKSNPVEVSAEEKAIEDNKVSEKSEVEKTEETTADETFVDLAEKF
                     IDQGIVTKDSKGYKLESEYIGKKKNDVAKSLSELPAEKLEELKALLD"
     gene            4533..5078
                     /locus_tag="PKX59_08730"
     CDS             4533..5078
                     /locus_tag="PKX59_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90989.1"
                     /translation="MAIGTVIQLVKERLPFIPNDNTYDARIESLKLEVYYALQHCTKL
                     TDAEVEDDSKYTGLRRFLVARYVCYLLIYNKIIEVVGGVGGSAPSGNKRIKKGKADVV
                     EAEFDYAKASDGTTLTMEATNLMNGLLKEICSYASTLKCSLPLCSGIIKSKGYGPAFI
                     AYGGTSNICGCGSSSNPQNDY"
     gene            5081..5536
                     /locus_tag="PKX59_08735"
     CDS             5081..5536
                     /locus_tag="PKX59_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90990.1"
                     /translation="MASLLTDSEFDEIRDTIKDVTDTFLRLPIVWKKDKNTATRFMRD
                     IDGERTYDDIALSGLVVWNDQGNGALLSKGLNGAIDLADGYVLLNYEDAQTAGVVSST
                     HNQIVNPAKDYLVFDNEKHEILSLNMLGQLKDKNCLLKVHFKKNIRPNG"
     gene            5529..6023
                     /locus_tag="PKX59_08740"
     CDS             5529..6023
                     /locus_tag="PKX59_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90991.1"
                     /translation="MAKNIKKVGRWDLAGMVTKNLKHDIKDSNLIVNRKMGSLLERYV
                     IKWIKRQPGLWAPLNEKYKKRKEKQGYSNLTLRRTGTLINNITSVASMDTIFCGVKRE
                     VKYNDGESVANIALIMEVGSAKRNIPPRPYLTPAMRWMGMKIRTEKLYEVMLMDYIRK
                     KYGI"
     gene            6020..6772
                     /locus_tag="PKX59_08745"
     CDS             6020..6772
                     /locus_tag="PKX59_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90992.1"
                     /translation="MKFKQSEISDGIFEVIRKAVVAGGYLPDFVSAGSSSAYQSAKGA
                     IISSGKEVIEVFNVGTYKSKGEDMNNNIIINRASVVPAKIGTGKEIAFVQNDNGTFDK
                     IHTPDTRYDIMYSISYNTISEKYAEIIEDIILNAFESRKFIRAVRSNGDIIMNVLDDY
                     NNDLSDDFGGSIESIENVAGFWLMNRGFVDTSGTNFIEKQWRFEAINIDLIGWKNIDT
                     VPQLVQFDFALGTEPNNTNAGNEDLFNIDLEI"
     gene            6829..8790
                     /locus_tag="PKX59_08750"
     CDS             6829..8790
                     /locus_tag="PKX59_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90993.1"
                     /translation="MTNLSGTPGASTNISDVGVIPANSNKGIICVNIIAKRGKLGKQY
                     LITNATELKRILGGAIPGNQDFYKLSRGLNKGAKLRVARVFHLTDQDDATTVEGDVAT
                     VTATQNVTEVLARANAKLTTVPSGDVVVQYVVNTVSGEVVIASYTIPTSGSPTVATVN
                     TALYTAVNAGTGTHGYTATIDGGYIVTVIAPTGSGEDANRYFLNVVQTGATAGTVAGN
                     GRFAGGVTGVDASVEIDAEAVGSGYNGTVIEIVKSISNQPGKVDIKVTLPDNDLPIQV
                     SNYDRVLTSETCASLNGQIGVVAFDFSSMNVGNMLPIGTFILADGTQDVSLINDADFI
                     GSPISKNGYHSFNDVSDSMRIWNVSKPTHTANQGLVAYVMARGDMRCRIYTPLGLSIE
                     GITDFRNGTGSFSHQPIDSFYGDMWYTDIEINDPANSSNTGYVISGIGDQLANRTLAD
                     DKAGVWISDSGEMFGKLFGVNDVAINFKSPAYKGYYDDLYEGGVNAIVNHPTFKITNW
                     GNRTLNLNRTSLLSKTNIADLVVYISREVKLIAERMSFNPNDLVMFGRLYRKVRPFII
                     DELIAGRAIEGGTGPKYGEGTWWHWLGDQNAKNLNELQFNTKSDVDAGKYRVRFAFKP
                     IASNEYIAIDIAPADSVTIQNIQQLLTVN"
     gene            8801..>9205
                     /locus_tag="PKX59_08755"
     CDS             8801..>9205
                     /locus_tag="PKX59_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ90994.1"
                     /translation="MAINNPQGNFNWRLDLDDVSNERLQNVQLPSVEWTEHKQGTQGN
                     DPDTKTPGKKIVGDMTAEMVVPDTGDTDLWNKLNKAKTMKRSQYCGNGVLYELDANGQ
                     PVQTHILENVWIKKVETANYETKGDNSDNLIRT"
BASE COUNT         3137 a         1533 c         2107 g         2428 t
ORIGIN      
        1 gtttttttag atagttattt tgagtacagg aatgaatgca aaaatgcagg tatagttccg
       61 gaatcatgcg agtattatta tcacaacaaa acagaatgca gaaaaaaatt aaacgaaaat
      121 taagtaacta ttgattatat caaactgaag tacgatattt gtgcaaatca atacacaggt
      181 aaattaaagt tatggattca acaacggaaa aaacattaca ggatttaggt cggaaagtaa
      241 aaatgctgcg agaggcaaag gatatcagtc aggatgagtt ggcaagtaag ctgaacctgg
      301 taaacggcca ttcttatatc tcgaaaattg aaaaaggtgc agtaaacatc acgatagaaa
      361 aaatgtgtga ggtgttatct gtattggatt gcgagtttac tgtattcgta aaaccgaatt
      421 tcaaattgag ccaaaataaa gccaaaaaaa cgaatgaaaa aaaaggagtg ctcaaaccca
      481 agaagataag ttttacaagt taaatcaaag tacatacaag ctaaatgaac ggcatagtgg
      541 aggatatctg ctatgccgtt ttgatttata acgatttttt cattatactt agagataaag
      601 taaagttact tatgtctaag aaagggaaaa ttacaatgtt taataatacc aatggtttac
      661 tgttggcacc caaatttatt gaacttgata gcggtaagaa acagtcattt agttgggata
      721 atgaggacta caggcagtca gtcgtaaatc ccgatatctc caatgcgatt gcaaaaaagg
      781 aggatttttt agcgttcaat ttccgtcaca tatctgcaac cattgtagga gcatggagtt
      841 ggaaagctac cgagtttaat gagaaagttt tgaaagaggc taaatcattg ctcgaatata
      901 aacctgcatt agtaaatcac aatgcggaaa caggtaatat aattggtgtt aatggtaaag
      961 tggtttatga gaatgcatac aaggcaaatg acggcaccat tattcctgca ggactgaatg
     1021 cacctatttg gattgacggc aagttacata cggatatatg ccgtaaatta tcggcattcc
     1081 cggtgccaca tatccaatcg gtatcagtat ctctcttatt cgagtgggag ccgtcacata
     1141 cgtttacgga ccaaagcggt aacgaggatg attggatgtt cgaaatgcgt atcggtaccc
     1201 tggttgacgg tacaatggta agacgtgtgg ttactaaaat tgtggacttc tatgaaacat
     1261 cattggtatt tttaggagca gatccgtttg caaaaatagt agataccgag ggcaagttgg
     1321 tgaatgtaga gaaatctgca atcgttggca gacagatgtt ttcggatgat aaaattggta
     1381 gcgattatta caaaagaagt aacggccatt actatttaat ggacgatgtt acctttgcaa
     1441 acgaaaaaag tataaattta agagaacgaa ttaacgtcga tttttcaaaa aatggaaatt
     1501 cacccaaaaa taacgaaatg aaagtattaa aattatccgg caaaaaagaa gattacgcac
     1561 aggaagtgct tactacctta gaagaatctt tcaaagttga atttgttgag ttgaaagacg
     1621 aaacagaaac aatcacaaac ctgacaaaag aggtggagga gttcaaatct gcaaagacta
     1681 ctgcagaaga atctttggct aaagttcaaa gcgaattaac tgaagcgaaa aatgcatttg
     1741 ctgaatacga gaaaatcgtt ccggtagctg aactttccga gtttgcaaaa gttatcgacc
     1801 tggcacaagt aaaacctttg gcagagttcg gacgtaaggt tgaattatcc aagcgtgagg
     1861 agtgcatccg tttatacaag gtttctgtaa aagacaaaac caatgatgca gtcattaaga
     1921 caatcaatga agcgagtttc gatgcattgg agggattgat gcaacagtat ggcggtacct
     1981 gcatggagca gttcggtgga aaatgtgacg aatgtaattc tactaaagtg agtttccgtt
     2041 cttcagtgaa cgagggtgag cagaaagagg aaacagagaa aacgtcattc gtttctatgg
     2101 cagaacaaat cagaaattaa ttatttattt tatcaaatta taaaaatcaa aaaagatgac
     2161 aggttctatc caaacaaccc ctacacaagt cataaaaaac atgacttcag atgcgttaca
     2221 actaactttt gtaagttcag caggtagcga aataaaagca ggacaggagg tatatcaaca
     2281 tactgacggt accgttaaaa aacgtacatt aggtacgcaa attcctttgg gaattgcatt
     2341 atctgatact ccggacggag agcgtgtatt agtgcgtgtg cctcatcaaa tggttatcga
     2401 gggtattgcg aaaggcggtg ctttaaatgc aggtgttgca gtagttcctg acggtacatt
     2461 aaatgcagac ggatatccga actatgtagc agccggtgca tcagcctatg tgtcagcatt
     2521 cacattgaaa gcagcagttg aagatgcaag agttcgtatc ggtatcattc agggtgtgta
     2581 tttaacgcct ggagcataat cgttaatcgt aatcaattag taatcgtata aatcaaataa
     2641 aatgaaagaa aacctattaa agttgagaaa acacaactta gcagtattca aaggtgataa
     2701 aactgacaga cttcaagcag atgaagtaaa gacgttaacc gaagaaacga tcccggattt
     2761 tgcaaaagaa attgcgttaa ctagacaagg tagagcaacc gatgcttacg gtcgtgttat
     2821 cccggcatac aatatttcgt ttgcagatgc tatcagcatc cactatggta ttcagccggc
     2881 aaagcaaacc gaaagtggcg aaaagttaag ctacaagcaa caacaaaagc atgttgttaa
     2941 ggagttcctg aagcaaatgg atattttcat gggttccgat actctgaaaa ctgcggccgc
     3001 tcgtttgggt aatgcgaatt taggtgttca gtctttgaca gatgcactta ctaaatattc
     3061 acagttcgat gcattagcga atacctccgg tgttcctgca gactaccgtt tcatcattcc
     3121 tgaattaatc ttaacggcta tccgtttgga ttacgatgca agttcattgc accagggttg
     3181 ggttgccacg acgcaatcaa tctcacagga cgaaatcaca atgccgttca tcaagaaagg
     3241 taatgcggtt gcacgtaaag ttggtgaggg tgaaagtatc gaatttggtt ccgtatcatt
     3301 cggtaaaaag aaagccggtg tattcaaagt tggtttgggt ttcaaaatca cagacgaact
     3361 gattgagcgt tcatctttgg acatgatttt cacgttctta ggtgaggtgg gtgtagatat
     3421 gtcaatctgc gctgactatg aggcttacag agtattggtg aacggtgagc aatcttcagg
     3481 tgcagaatcc gctccgacta tcggtgttgc gactgccaac acattcaact tcaaagacat
     3541 caaacgtggt acgtcccgtt tacgtcgatt gaaaagaatg cctgatacaa tcattacagg
     3601 tgaaaacgac ggtatcgata tcacttcatt accggagttt cagggtttca atggtggcaa
     3661 caaaatggca actattcagt caatcttagg agtgcctgat acattgaaaa acgacgtgta
     3721 cacagttgga agcaatcaga tcatgttgtt agacagtaca aaagcaatgg cgaaattgca
     3781 gtacggttct atgaaaatgg aggaaagaag aaatccacaa acacaggaga acgaaatctt
     3841 tgtaagcgac tatatcggat tttcaattat cagacgtgat gcacgtttaa tcattgacaa
     3901 atcagtattg ttcagcgcac aaggtttccc tgcatacatg gatatcgacg cacgtatcaa
     3961 cgaggcgttc aaataccatg aggagtaata tttttcacca ttaaataaat agtatggaaa
     4021 tttttgtaaa gttgaaagat gcaagtaact cacacagtat attgcatcag caaaaaaacc
     4081 tgttcggtaa aaaagttgag aaagttctga aagacgttta cgtttcaaaa cttttgaaag
     4141 acggagttat cgtggaggca aaagaaacgg aatacgacgc ttgggtaaag agcaatccgg
     4201 ttgaagtttc tgctgaagaa aaagctatcg aggataacaa ggtaagcgaa aaatcagaag
     4261 tggaaaaaac agaagaaaca actgcagatg aaacgttcgt ggatttggca gagaagttca
     4321 tcgaccaggg tatcgtaacc aaagacagta aagggtacaa actcgaaagt gagtatatcg
     4381 gaaagaagaa aaatgatgtc gcaaaatcat tatcggaatt accggcagaa aagttggaag
     4441 aattgaaagc cttattggac taagatttat tcatacagaa aaagaaaaac ggatgctcac
     4501 agtatccgtt ttttttattt atatttagta ttatggcaat aggaacagta atacaattag
     4561 tgaaagagcg actacctttc atacctaatg ataacacgta tgacgctcga atagaatctc
     4621 tgaaattaga ggtttattat gcacttcagc attgcacaaa attgacagat gcagaggtag
     4681 aggacgattc gaaatatacc ggcctccgga ggttcttagt ggcacgatat gtttgctacc
     4741 tgcttatcta caataaaatc attgaggtag ttggtggtgt aggaggtagt gctccgtccg
     4801 ggaacaagcg tatcaaaaaa ggtaaagctg acgttgttga ggccgaattt gactatgcaa
     4861 aagcatccga cggtaccaca ttgactatgg aggcaacaaa cttgatgaat ggcctgttaa
     4921 aagagatttg cagttatgca agtacgttga aatgtagcct accattatgc tccggcatta
     4981 taaagagcaa aggttacggc cctgcattca tagcgtatgg tggcacgtcg aatatctgcg
     5041 gttgtggcag tagttctaat cctcaaaacg actattaatt atggcatcat tactgacaga
     5101 tagcgaattt gatgagatac gtgatacgat taaagacgtt accgatactt tcctgcgttt
     5161 acctatcgta tggaagaaag acaagaatac tgcaaccagg ttcatgcgtg acatcgacgg
     5221 agagcgtaca tacgacgata tagcattgtc gggattggtt gtttggaacg atcaagggaa
     5281 cggagcattg ttgagcaaag gattgaatgg tgccattgat ttagccgacg gttatgtatt
     5341 gctgaattac gaagatgcac aaactgcagg tgtagtttcc tccacacata accaaatcgt
     5401 aaatcctgcg aaagattatc tcgttttcga taatgaaaag catgagattt tatcgttgaa
     5461 tatgttagga caactgaaag acaaaaactg tttattgaaa gttcacttta agaagaatat
     5521 caggccaaat ggctaagaat ataaaaaaag ttggcaggtg ggatttagcc ggtatggtta
     5581 caaaaaatct gaagcatgac attaaagata gtaaccttat cgtgaacagg aaaatgggtt
     5641 ccttattgga gcggtacgtc ataaagtgga ttaagcgcca acctgggttg tgggcaccat
     5701 tgaacgagaa gtacaaaaag cggaaagaga aacagggtta ttcaaatctg acattgaggc
     5761 gtacaggtac gttgattaat aatatcacat cggtagcaag tatggacacg atcttctgcg
     5821 gagtaaagcg tgaagtaaag tacaatgacg gagagagcgt ggcgaacatt gcgttgatta
     5881 tggaagtagg tagcgcaaag cgtaacattc ctccacgacc atatttaact ccggccatga
     5941 ggtggatggg tatgaaaatc agaacggaga aattgtacga agtaatgctc atggattata
     6001 tcagaaaaaa atacggaata tgaaattcaa gcaatcagaa ataagcgacg gtatattcga
     6061 agtaatacgc aaagcggtgg tggccggcgg ttatctgcct gactttgttt cagccggtag
     6121 cagtagtgca tatcaatctg ccaaaggagc aattatcagt tccggtaaag aagtaattga
     6181 agtattcaat gtcggtacct ataagagcaa gggtgaggat atgaataata acattattat
     6241 caatagagca tcggttgtcc ctgcaaagat tggtacaggt aaagaaatag cattcgttca
     6301 aaatgataat ggaacgtttg ataaaattca cactcccgac actcgatatg acattatgta
     6361 tagtatatcg tacaatacaa ttagcgaaaa atacgctgaa attattgagg atattatact
     6421 gaatgcattt gaatcaagaa agtttataag agcggtaagg agcaatggtg atataataat
     6481 gaacgtttta gatgattaca ataacgacct atctgacgac tttggtggta gtattgaatc
     6541 aatagaaaac gttgcaggtt tttggcttat gaatcgtgga tttgttgata cttcaggtac
     6601 taattttatt gagaaacagt ggaggtttga agctattaat attgatttaa ttggttggaa
     6661 aaatattgat acggtaccac aattagttca gtttgatttc gctttaggca ccgagcctaa
     6721 caatacaaat gcaggtaatg aggatttatt caacatagat ttagaaattt aaaagtattt
     6781 gtataaatta cagtataacg ataatctaaa aatttaaaat tcaggaaaat gacaaactta
     6841 tcaggaactc ccggagcatc taccaacata tcagatgtag gcgtaatacc ggcaaatagc
     6901 aataaaggaa ttatttgtgt aaacatcatt gccaaaagag gaaagttagg taagcaatat
     6961 ttgattacca atgctaccga attgaaaaga atattaggtg gtgcgattcc cgggaatcaa
     7021 gacttctaca agttgtcaag aggcttaaac aaaggagcaa agttgcgagt agcgagagtt
     7081 tttcatttga cagatcagga cgacgcaact accgttgagg gtgacgttgc aacagttacg
     7141 gccacacaaa acgttactga agtattagcg agagcaaatg caaaattgac taccgttccg
     7201 agcggtgacg ttgtggttca gtatgtagtg aatacagtaa gtggtgaggt agtgattgca
     7261 tcgtacacaa ttccgacatc aggtagtcca acggtagcaa ctgtaaatac ggcattatat
     7321 actgcggtaa atgcaggtac aggtacgcat ggttatactg ctacaatcga cggtggttac
     7381 atcgttacgg tgattgcacc aaccggttcc ggagaagatg cgaacagata tttcctgaac
     7441 gtggtacaga caggagcaac tgcaggaaca gtagccggta atggccgttt tgcaggtggt
     7501 gttacaggtg ttgacgcatc ggtggaaatt gatgctgaag cagtaggctc cggttacaat
     7561 ggtacagtaa ttgaaatcgt aaaatcaatt tcaaatcagc cgggtaaagt tgacatcaaa
     7621 gtaacgttac ccgataacga tttaccaata caagtgagca actatgacag agtgctaaca
     7681 tctgaaacgt gtgcatcttt gaacggacaa atcggtgtag tagcattcga cttcagttca
     7741 atgaatgtag gtaatatgtt acctattggt acgtttattt tagcagacgg tacacaggac
     7801 gtatcattga ttaatgatgc tgattttatc ggtagtccaa tatctaaaaa cggatatcac
     7861 tcattcaatg acgtgagtga cagtatgcgt atttggaacg tttcaaagcc tacacatact
     7921 gctaatcagg gattggttgc atacgttatg gctcgtggtg atatgcgctg cagaatttat
     7981 actccgttag gattaagcat tgaaggtatc actgatttca gaaacggtac cggctcattc
     8041 tctcatcagc cgattgattc tttttacgga gatatgtggt acacagacat cgaaataaac
     8101 gatcctgcaa attcctctaa tacaggatat gtaataagcg gtattggcga ccaattagct
     8161 aacagaacat tagcagacga taaagcaggt gtttggatat ctgattccgg tgaaatgttc
     8221 ggtaaattat tcggtgtgaa tgacgtggcg attaacttca aatctcctgc atacaaaggt
     8281 tattacgacg atttgtatga gggtggtgtg aatgctatcg taaatcatcc gacgttcaaa
     8341 atcacgaatt ggggtaacag aacattgaac ctgaaccgta cctctttatt gagcaaaaca
     8401 aacattgccg acctggtggt ttatatttca agagaggtaa agctgattgc agagcgtatg
     8461 tctttcaatc cgaatgattt ggttatgttc ggaagattat acagaaaggt gcgtccgttc
     8521 attatcgacg agttgattgc cggtagagca atcgagggtg gtaccggccc gaaatacggt
     8581 gagggaacat ggtggcattg gttaggcgac caaaacgcta agaacctgaa tgaacttcag
     8641 ttcaacacaa agagcgacgt agatgcaggt aaatatcgtg ttcgttttgc attcaagccg
     8701 attgcatcaa acgaatacat agctatcgac attgcacctg cagatagcgt aactattcaa
     8761 aacattcaac aattattaac agtaaactaa ttcaaaagtc atggcaataa ataatccaca
     8821 aggcaacttt aattggcgtt tagatttgga cgatgtatca aacgaacgtt tacaaaacgt
     8881 acaactgccg tcggtagaat ggacggagca taagcagggt acccagggta acgatcccga
     8941 tacgaaaact ccgggcaaga aaatcgtagg agatatgact gcagaaatgg tagttcccga
     9001 tacaggtgat accgatttat ggaacaagct gaataaagca aaaaccatga agcgttcgca
     9061 gtattgcggt aacggtgttc tgtatgaatt ggatgcgaac ggacaaccgg tgcagacgca
     9121 tattttggag aacgtgtgga ttaagaaagt ggagaccgct aattatgaaa cgaaaggaga
     9181 taactccgat aaccttatac gaacc
//
LOCUS       DAOOOI010000076         8869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1553_length_8869_cov_10.714658, whole genome shotgun sequence.
ACCESSION   DAOOOI010000076 DAOOOI010000000
VERSION     DAOOOI010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1553_length_8869_cov_10.714658"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    238..288
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<289..1471)
                     /locus_tag="PKX59_08760"
     CDS             complement(<289..1471)
                     /locus_tag="PKX59_08760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOZ90995.1"
                     /translation="MQRVVITGIGIYSCIGKNLAEVTESLRQGKSGIIFDQERKDLGF
                     LSAMTAFLETPDLKDFLHRRDRLGMHQPAMYAFMSTREALAMAKLDTDYMEKNETGII
                     FGNDSTAGSVVETIDKARKKKDNTLIGSGDIFQNMNCTVNMNLATIYKLKGINFTLSA
                     ACASGSHAIGMGYLLIRQGLQERVICGGAQEINPPAMTSFDGLGTFSSRENEPQKASR
                     PFDKSRDGLIPSGGAATVILESLESAQKRGATILAEVTGYGFSSNGDHISNPDFNGQE
                     RSLNMAMKLAGINASNIDYVNAHATSTPAGDGIEAKVISHVLGNKVPVSSTKSMTGHE
                     CWMAGASEVVYSMLMMQNDFVAPNINFEEPDEDTKAINIITSSMEHRINCFLSNSFGF
                     GG"
     gene            complement(1475..2206)
                     /gene="fabG"
                     /locus_tag="PKX59_08765"
     CDS             complement(1475..2206)
                     /gene="fabG"
                     /locus_tag="PKX59_08765"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOZ90996.1"
                     /translation="MNCALVTGASRGLGKAIALQLAADHKLHILINYASNKQAAEETL
                     TTIKNAGGSGELLPFNVQDNETVNAALDHWHKENENKFISVLVNNAGITRDGLFMWMP
                     EKDWDDVLNISLKGFYNVTRNVVNPMLRKRAGRIVNIVSLSGLKGNAGQTNYAAAKGA
                     IVAATKSLAFEVAKRKITVNAVAPGFIKSDMTEGLNEKELSKLVPMERFGTPEEVAHV
                     VSFLASDKASYITGEIININGGIYS"
     gene            complement(2210..3751)
                     /locus_tag="PKX59_08770"
     CDS             complement(2210..3751)
                     /locus_tag="PKX59_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HOZ90997.1"
                     /translation="MRIRSMANPLTLAELESIAFNRKPVTVDDEIIKSINQSFDFLVR
                     FSKDKIIYGINTGFGPMVQYKVENHELNQLQYNLIRSHSSGTGAVLNEVYARAVVAAR
                     LNNFLQAHSGVSPEVISLLTTMLNENIVPEIYEHGGVGASGDLVQLAHLGLNLIGEGY
                     VHYDGKRQKTADVFKKLNIKPLTLQLRDGLGIINGTSCMTGIAAINLVYAKRLTRWMI
                     SLSAVINEIIESFDDSFSAELNHVKLHNGQREVAQQMREFLKGSKLVRSRKELFSDHA
                     ELSRTEFKNKIQEYYSIRCVPQIIGPIYDTCMAAIAVTENELNSTNDNPVVMPEADNV
                     FHGGNFHGDYISLEMDKLKLAISKLSVLAERQLNFLLNKKLNRKFPPFLNKKKLGFNF
                     GLQGMQFLATSTTAENQSLSNSVYVHSISNNNDNQDVVSMGTNSAIIARKVIDNSYEV
                     VAVLTLAVCQAIDLLSTEESQKLCKNTLSLHGLIRKQVAVVEDDVPQFENIRKVIDIL
                     KENEL"
     gene            3868..6702
                     /locus_tag="PKX59_08775"
     CDS             3868..6702
                     /locus_tag="PKX59_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ90998.1"
                     /translation="MRIFFTAFLLFISGYVSIAQNVISGVIKDGSSGETLIGATIVIK
                     GTTEGAATDIDGKFSFETTRSFPLVLVASFVGFINQEITINNISPLTIKLKKNEVLLK
                     NVTVTGSRISEKQKESPITVEALDYIAIKETPAASFYEGLGQLKGVDLTSASLGFKII
                     NTRGFNSSSPVRSLQLIDGVDNQSPGLNFSLGNFLGSSELDVQKVDLIVGASSAYYGP
                     NAFNGVIAMTTRSPFESPGLQFMVKIGDRKLNEFALRFAQVFKNKKGENKFAYKLNFY
                     RFTALDWKADNYSPTEQSRNTSYNPGGYDAVNVYGDEDINGTDYRNFPGAFPGLMSFN
                     RKGYKEEDLVDYNSNNTKAGLALHYMITPKIEAITSANYGTGTTIYQGDNRYSLKNIK
                     FLQNRVEVREKDKWFIRAYSTSEDAGQSYDAYFTALLLQRSAKSDGHWKNDYENFWNG
                     NYNMSYIRKLPGFPPPPGFGPAYVQWLQSVNPFLLNNYYDSLVHYHQQAQAYASGVGN
                     PQNQNLAFFEPGTARFDSAFNAITSRKSYSEGGSRFYDKSKLYHIQGEYRYPNKWVDG
                     VVGGNYRLYKPNSDGTIFSDTSYVHYNISANNDTLSIDTLRNKLSVYEFGVYAGLEKK
                     ILKDKLKLNATVRFDKNQNFKLLVSPAGSVVYTPNNNHVIRVSFSSAVRNPTLTDQYL
                     FYQVGRATLLGNINGYTNLLTPESVTEFYDSSKSSFKFLNFFNIDPVRPEKVKTIEAG
                     YRATLFNRLYVDLNGYYSWYRDFIGYQIGVDADTFSVNTFGGTIYDLVFNRVVRVASN
                     AQSEVTTQGVSVGLNYYIGKYFALTGNYSWNKLNKKGTDDPLIPAFNTPENKYNIGFN
                     GRDIKGVGFNVNYKWVQGFRYEGSPQFTGEIPSYDMVDAQVNYHLKEAGTTFKLGAMN
                     LFNNKHYEIYGGPKVGRMVYFSILFEVDKL"
     gene            6733..7221
                     /locus_tag="PKX59_08780"
     CDS             6733..7221
                     /locus_tag="PKX59_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOZ90999.1"
                     /translation="MKTSAKSVDEILSNVQEDRKEVFQKLHQVILKNLPKGFEAAISY
                     GGLGYVVPHSIYPDGYHCKPVEPLPFAGIASQKQSINFYHMGMYSDPVLLKWFVSEFP
                     KHSNQKLDMGKSCVRFKKMDDIPYKLIGELMKKMTVKEWIQKYESAYKPKPKEVKTKK
                     GK"
     gene            complement(7292..8416)
                     /locus_tag="PKX59_08785"
     CDS             complement(7292..8416)
                     /locus_tag="PKX59_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ91000.1"
                     /translation="MKRILTVAFISLTLGGFAQRAQVQSAYSYLKYEQLDKAKECIDI
                     AVNNENTMNYDKAWYYRGLIYQALYKNEKFANLSNDPLNEALRSYNKALELNPKFEYA
                     DDISEKKKILAQQFADMGYVNYNLKNYAGALTAYEGVVSIMPNDTSSYFNAALCAERA
                     GNAAKAKQYYNKLDEMQYKDAKMYHSYAQLYLSEGDTAKALEVLSRGLSRFPEEKSIL
                     ITQTNIYISSGKTAEALSAINMAIEKDPTNANLYFAKGTLVEKTEKSKDAAIAAYKKA
                     IELKADYFDAYYNLGALYFNDGAHLANEANSIKDNNQYAKAKAVFEAKFKEAKPYLEK
                     AWELNPKDQSTMASLKQLYATINDTVNYAKVKAALDAETK"
     gene            complement(8441..>8869)
                     /locus_tag="PKX59_08790"
     CDS             complement(8441..>8869)
                     /locus_tag="PKX59_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOZ91001.1"
                     /translation="NATEETILVVSEKGYGKRSDLEDYRVTNRGGKGVKTMNITEKTG
                     DLIAIKSVKEDDDLMIINKSGITIRMEVKDLRVMGRNTQGVKLIRLDDDDSIASVAKI
                     DMEITGNGNQKENGNDTEENPTIENGEQNNNQTEENNNPE"
BASE COUNT         2586 a         1634 c         1680 g         2918 t
ORIGIN      
        1 attgtctatt ttgagatttg ttgattacaa gtattgaatg tagaggatat ttaaaggtta
       61 agagtttatg gaattagtaa acagaaacta cattagatga aaataagatg gttatgttaa
      121 gtgacactta aatacttttt ataaagtaaa ctgaaagaaa aacaaaaata atcaggtcag
      181 tccacaaggc aaatttttca tttattcctg aagttttatt aaatccatgt tgtatggnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta ccaccaaacc
      301 caaatgaatt agaaagaaaa cagttgatac gatgttccat tgaagaagtg atgatgttga
      361 tggctttagt atcttcatca ggctcttcaa aattaatgtt tggagccaca aaatcattct
      421 gcatcatcag catagaatat actacctcgc tggcacctgc catccaacac tcatgtcctg
      481 tcattgattt ggtagagctt acaggaactt tgtttcctaa aacatgtgag atcacttttg
      541 cttcaatgcc atcgcctgca ggtgttgagg tagcatgggc atttacgtag tcaatatttg
      601 atgcattaat tcctgctaac ttcattgcca tgtttaatga gcgttcttgc ccattaaaat
      661 caggatttga aatatgatcg ccattggatg aaaatccata gcctgttact tcagcaagta
      721 tggtagcacc tcttttctgt gccgattcaa gcgactctaa aattacagtg gcagcaccac
      781 ctgaaggaat tagtccgtca cggcttttat caaatggacg cgatgctttt tgtggttcat
      841 tctcacgact gctgaatgtg ccaagcccat cgaagctggt catagcaggt ggatttattt
      901 cttgtgcacc accgcagata acacgctcct gtaatccttg acgtatcaac aggtaaccca
      961 taccaatggc atgtgatcca cttgcgcaag ctgcactaag tgtaaaattt attcctttaa
     1021 gcttataaat agtggcaagg ttcatgttta ccgtgcagtt catgttttga aaaatatcgc
     1081 cactgcctat taaagtattg tctttctttt ttcttgcctt gtcaatagtt tctactacag
     1141 aacctgctgt gctgtcattg ccaaaaatga ttcctgtttc atttttttcc atatagtctg
     1201 tatcgagctt tgccatagca agtgcttcgc gtgttgacat gaatgcatac attgccggtt
     1261 ggtgcatacc caaacggtca cgtctgtgaa gaaaatcttt aaggtcaggt gtttctaaaa
     1321 aagctgtcat tgcagaaagg aatcctaaat cttttctttc ctgatcaaag ataattccac
     1381 tctttccctg acgtaacgac tctgtaactt ctgcaaggtt cttgccgata catgaataaa
     1441 taccgatacc tgtgatgact actctttgca taaatcagga ataaatacct ccgttaatgt
     1501 tgatgatttc gcctgtaata taagaggctt tgtctgatgc cagaaagctg acaacatgtg
     1561 ctacttcttc aggtgtgcca aaacgttcca tcggaactaa tttacttaat tctttttcat
     1621 taagtccctc tgtcatatca ctcttgataa atccaggcgc aacagcgttg acagtgattt
     1681 ttcttttagc aacctcaaaa gcaagtgatt ttgttgcagc aacaatagca cctttggcag
     1741 ccgcataatt tgtttgtccg gcatttcctt tcaatcctga gagtgaaaca atattgacaa
     1801 tacggcctgc gcgtttgcga agcattgggt tcacaacgtt tcgggtaaca ttataaaatc
     1861 ctttgagtga aatatttaaa acatcatccc aatctttttc tggcatccac ataaaaagac
     1921 catcacgggt gatgcctgca ttgtttacca atacactgat aaatttattt tcattctctt
     1981 tatgccaatg gtcaagagca gcatttactg tttcattatc ctgaacatta aatggcagca
     2041 actcaccact acctccggca tttttaattg ttgtcaatgt ttcttcagca gcttgtttgt
     2101 ttgatgcata gttgatgaga atgtgcagct tatgatctgc tgcaagctga agtgcaatgg
     2161 ctttgccaag tcctcttgat gctccggtta ctaaagcgca attcatgatt taaagttcgt
     2221 tttcttttaa aatatcaata acttttctga tgttttcaaa ctgtggaaca tcatcttcaa
     2281 ctacggcaac ctgcttacgg attaaaccat gcagtgacaa agtgtttttg caaagttttt
     2341 gagattcttc agtacttaac aagtctattg cctgacatac agcaagtgtt agcacagcaa
     2401 caacttcata gctgttatca attacttttc tggcaatgat ggcgctgttg gttcccatac
     2461 tcaccacgtc ctgattgtcg ttgttgtttg aaatgctgtg aacataaaca gagttgctga
     2521 gcgactgatt ttctgctgta gtactcgttg caagaaactg catgccctgc aacccaaaat
     2581 taaatcctag ttttttcttg ttcagaaatg gaggaaattt tctgttgagt tttttgttca
     2641 ataaaaagtt tagctgacgt tcggcaagta cagaaagctt agaaatggca agtttaagtt
     2701 tatccatttc aagagaaata taatcaccat gaaagttgcc gccatgaaac acattgtctg
     2761 cttcgggcat gactacagga ttgtcgttgg ttgaatttaa ttcgttttca gtaacggcaa
     2821 tagcagccat gcaggtatca taaataggtc ctatgatttg tggtacacag cgaatggaat
     2881 aatattcctg aattttgttt ttgaattctg tacggctcag ttcggcatga tcagagaaga
     2941 gctcttttct gctacgcacc aatttacttc ccttcaaaaa ttcgcgcatt tgttgagcaa
     3001 cctcacgttg tccgttgtgt agcttaacgt gattgagttc agcagaaaaa gaatcatcaa
     3061 acgattcaat aatttcgttg atcactgccg ataaagatat catccagcga gtaagtcgct
     3121 ttgcataaac aaggttgatg gctgcaatgc ctgtcatgca cgatgtgcca ttgataatgc
     3181 ctaagccatc acgcagttgt agtgttaatg gttttatgtt taacttctta aaaacatctg
     3241 ctgttttttg acgcttacca tcataatgaa cataaccttc gcctatcaga tttagtccaa
     3301 ggtgtgcaag ctgaaccaaa tcgccactgg caccaacgcc accatgctca taaatttcag
     3361 gaacaatatt ttcattcagc attgtggtga gcaatgaaat tacttcgggg cttacacctg
     3421 aatgtgcctg aagaaaatta ttaagacgtg ctgccacaac agcccttgca tatacttcat
     3481 tgagtactgc acctgtgcca ctgctgtgac tgcgtatcag attatattgt aactgattta
     3541 attcgtgatt ttcaaccttg tactgcacca tagggccaaa acccgtattg atgccataga
     3601 tgattttatc ttttgaaaac ctaactaaaa aatcaaatga ctgattgatt gatttgatga
     3661 tttcatcatc aaccgttaca ggtttcctgt taaatgcaat tgattcaagc tctgccagtg
     3721 tcagcggatt agccatactt cttatcctca ttaataggat ggcaaatgta atattttcaa
     3781 aaatatataa tgcccgaatc agacattgtt gtactattgt ttattcggtt tcggtatttt
     3841 acttttaccc caaataaaac caagtttatg aggatatttt ttacagcctt tttacttttt
     3901 atttccggtt atgtttctat tgcacaaaat gtgatatccg gtgttataaa agacggcagc
     3961 agtggcgaaa cattgatagg tgctacaatc gtcatcaaag gaacaacaga gggcgcagct
     4021 acagatattg acggcaagtt ttcgtttgaa accacacgta gttttccttt ggttttagtg
     4081 gcttcttttg ttggttttat caatcaggaa attacaatca acaatatatc acctcttact
     4141 attaaattga aaaaaaatga ggtgttgctt aaaaatgtta ccgtaacagg cagccgcatt
     4201 tccgagaaac aaaaagaatc gccaattact gttgaagcac tcgattatat tgccattaaa
     4261 gaaacacctg ctgccagttt ttacgaaggc ttggggcagt tgaaaggagt tgatttgaca
     4321 agtgccagtc ttggatttaa aattattaat acaagaggat ttaacagcag ctcgccagtg
     4381 cgttcgttac agttaattga tggtgtagat aatcaatctc cgggattgaa tttttcattg
     4441 ggtaattttc ttggcagcag cgaacttgat gtgcaaaaag ttgatctgat tgtaggggca
     4501 agttctgcct actatggccc caatgctttt aatggtgtta tagctatgac aacacgcagc
     4561 ccttttgaat caccgggctt gcagtttatg gtaaaaattg gtgacagaaa actcaatgaa
     4621 tttgcattgc gttttgctca ggtttttaag aataagaaag gtgaaaataa gtttgcatat
     4681 aaactcaact tttatcgctt cactgcttta gactggaaag ccgataacta ttcgccaaca
     4741 gaacaatcac gtaatacaag ctataacccg ggcggctatg atgcagtaaa tgtttatggc
     4801 gatgaagata ttaatggaac agactataga aattttccgg gtgcatttcc cggactgatg
     4861 tcttttaacc gaaaaggcta caaagaagaa gatttggttg actacaacag taacaacacc
     4921 aaggcaggtc ttgctttgca ttatatgatt acaccaaaga tagaagctat tacttctgct
     4981 aattatggta caggtacaac aatttatcag ggcgataatc gctacagtct gaagaatatt
     5041 aaatttttac aaaacagagt tgaggtcaga gaaaaagata aatggtttat aagagcttac
     5101 agcacttctg aagatgcagg acagtcttat gatgcatatt ttacagcact attattacag
     5161 cgtagtgcaa agagtgatgg tcattggaaa aatgactatg agaatttctg gaatggaaat
     5221 tataacatga gttacatcag aaagctgcca ggttttccgc cacctccagg ttttggtcct
     5281 gcttatgttc aatggcttca atctgtgaat ccattcttgt taaacaacta ttatgattca
     5341 ttggtgcatt atcatcagca ggcacaggcc tatgccagtg gtgttggaaa tcctcaaaat
     5401 caaaacctgg cattcttcga gccgggaact gcccgtttcg attctgcttt taatgccatc
     5461 acatcacgta agtcatattc agaaggggga tcacgttttt atgataagtc caaactttat
     5521 cacatacaag gtgaatatcg ttatccaaac aaatgggttg atggtgtagt aggaggaaac
     5581 tatcgtcttt acaaacctaa ttctgacgga acaatttttt ctgataccag ttatgtgcat
     5641 tataatattt cagcaaacaa cgatacatta tcaatagata ctttacgcaa taaactttct
     5701 gtttatgaat ttggtgttta tgcaggattg gaaaagaaaa ttctgaaaga caaattaaaa
     5761 ctcaacgcta cggtacgttt tgataaaaat caaaatttta aactgttagt ttctcctgca
     5821 ggttctgttg tttatacacc aaataacaat catgtcatca gagtttcgtt ttcatctgct
     5881 gtaagaaatc ctacacttac agatcaatat ttattttatc aggtaggaag ggctactctg
     5941 cttggaaaca tcaatggcta caccaacctt ttaactcccg aatcggtaac tgagttttat
     6001 gatagcagca agtcatcatt taaatttttg aacttcttta acattgatcc tgtaagaccg
     6061 gaaaaagtta aaaccattga agctggttat cgtgcaacac tgtttaaccg tttatatgtt
     6121 gatttgaatg gatattattc atggtatcgt gattttatcg gatatcagat aggtgttgat
     6181 gccgacactt tttctgtaaa cacttttggt ggtactattt acgatttggt ttttaatcgt
     6241 gtagtacgtg tggcttcaaa tgcacaaagt gaagttacta cacagggagt ttctgtcgga
     6301 ttgaattatt atatcggaaa atattttgcg cttactggaa attactcttg gaataaactg
     6361 aacaaaaagg gcactgatga tccattgatt cctgccttca acacaccgga gaataaatac
     6421 aatattggtt tcaacgggcg cgatataaaa ggtgttggtt ttaatgtgaa ctacaaatgg
     6481 gttcagggtt ttaggtatga aggctcacca caatttacag gcgaaattcc ttcgtacgat
     6541 atggttgatg cgcaagttaa ttatcatctg aaggaggcag gcacaacttt taagctgggt
     6601 gcaatgaact tgttcaacaa taagcactat gaaatttatg gcggacccaa agttggcaga
     6661 atggtttact tcagtatttt gtttgaggtg gataaattgt aatttttgaa actgttttaa
     6721 aaaagataag gaatgaaaac tagtgcaaaa agtgtagatg aaattttgtc aaacgtacag
     6781 gaagatagga aggaagtttt tcaaaaactg catcaggtaa tattaaaaaa tttaccaaaa
     6841 ggttttgagg cagcaatcag ttatggtggg ttaggttatg ttgtgccaca ttcaatttat
     6901 cctgatggct atcattgcaa acctgttgag cctttgcctt ttgcaggaat agcttctcaa
     6961 aagcaatcta taaattttta tcacatgggt atgtattccg accctgtttt gctaaaatgg
     7021 tttgtaagcg aatttccaaa acacagcaat caaaaattgg atatgggaaa aagctgtgtt
     7081 cgttttaaga agatggacga tattccatac aagctcattg gtgagttgat gaaaaaaatg
     7141 acggttaaag agtggattca aaaatatgaa tcagcctaca aacctaaacc taaggaagtg
     7201 aaaacgaaga agggaaaata ggataatgca agtaagagat aaaagcaaaa aagccccgca
     7261 ttgcggggct ttttttatgt gttcaaaaat tttattttgt ttctgcatct aatgctgctt
     7321 tcacctttgc gtagtttact gtgtcattga tagtggcata aagttgtttc agagaagcca
     7381 ttgtactctg atctttagga ttcaactccc atgctttttc aagataaggt tttgcttctt
     7441 taaactttgc ttcaaatact gccttagctt ttgcatattg attattgtct ttgatgctat
     7501 tagcttcatt ggctaaatgc gcaccatcgt taaaatagag tgcacccaag ttgtaatagg
     7561 catcaaaata atctgcttta agctcaattg cttttttgta ggcagcaatg gcggcatctt
     7621 tactcttttc agtcttctct actaatgtac cttttgcaaa ataaaggttg gcattggtag
     7681 gatctttttc tattgccata tttatggcgc taagtgcttc agcagttttt cctgagctga
     7741 tataaatgtt cgtctgtgta ataagaattg atttctcttc agggaagcgt gataatccac
     7801 gtgacaatac ttcaagtgct tttgctgtgt cgccttcgga aagatacagt tgtgcatagc
     7861 tgtgatacat ttttgcatct ttatattgca tctcatctaa tttgttgtag tactgttttg
     7921 ctttagccgc attgcctgca cgctctgcac acaatgctgc attgaaatat gatgatgtgt
     7981 cattaggcat gatagaaact actccttcat aagctgtcaa tgcacctgca tagtttttca
     8041 gattgtaatt tacataaccc atgtcggcaa attgctgagc taatattttt ttcttttctg
     8101 aaatatcgtc agcatattca aattttgggt tcagctctaa agctttattg taagaacgta
     8161 aagcttcgtt aagcggatcg tttgacagat tagcgaattt ttcattctta taaagtgcct
     8221 gataaatcag tccgcgatag taccatgctt tgtcatagtt catggtgttc tcgttgttta
     8281 cggcaatgtc aatacactct ttggctttgt ctaactgttc gtatttaaga tagctgtaag
     8341 ccgattgtac ctgtgcacgc tgtgcaaatc caccaagagt taaactgata aatgcaactg
     8401 ttagtattct tttcattttg ctaaattgtt tgattaataa ttattctgga ttattgtttt
     8461 cttctgtttg attattgttt tgttcgccat tctcaatagt cggattttct tctgtatcgt
     8521 tgccgttctc cttttgatta ccgttacctg taatttccat atcaattttt gcaaccgatg
     8581 caatggagtc atcatcatcc aatcgaataa gctttacgcc ctgtgtgttt cgtcccataa
     8641 cgcgaagatc ttttacctcc atacgaattg ttatgccgga tttgttgatg atcatcagat
     8701 catcatcttc tttaacactc tttatggcaa tgaggtcgcc tgttttttcg gtaatgttca
     8761 tggtcttaac acctttaccg ccacggtttg ttacacgata atcttccagg tcgctgcgtt
     8821 ttccataacc tttttcacta actactaaga tggtttcttc cgttgcatt
//
LOCUS       DAOOOI010000077         8771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1577_length_8771_cov_15.094539, whole genome shotgun sequence.
ACCESSION   DAOOOI010000077 DAOOOI010000000
VERSION     DAOOOI010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8771
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1577_length_8771_cov_15.094539"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="PKX59_08795"
     CDS             <1..842
                     /locus_tag="PKX59_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOZ91002.1"
                     /translation="DGSVYHLNLLPEDISDVVINVGDPDRVSKVSAFFDSIEVKKQKR
                     EFVTHTGHYKGRRISVISTGIGTDNIDIVYNELDALVNIDLKTRTVKSNLTALNLIRI
                     GTSGALQPEIPVDSFVFSTFGLGLDGLLNFYQYTNTPEEQAIIDSFRKHYPNNCKLPE
                     SYLTRSSHKLEQALSEGMHKGITASCSGFYAPQGRVLRYQLARTDMIEKLNSFRHDEH
                     KVTNFEMETGAMYGLAKLLGHHCCAVNLIVANRIAQQFSKKYEERMHQLIQTVLDRIS
                     AQL"
     gene            855..1766
                     /gene="gldA"
                     /locus_tag="PKX59_08800"
     CDS             855..1766
                     /gene="gldA"
                     /locus_tag="PKX59_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HOZ91003.1"
                     /translation="MSVSVKNVTKLYGKQRALDNVSFEINTGQVVGLLGPNGAGKSTL
                     MKIISCFIPQNEGKVSVCGFDTLSQSLEVRRNVGYLPEHNPLYPDMFVKEYLQFVGGI
                     YLPAKEAAKRADSMIALTGLTPEYKKKIGALSKGYRQRVGIAQALIHDPAVLILDEPT
                     SGLDPNQLVEIRALIQSIGKQKTVILSTHIMQEVEAICNRVIIINKGKIVADDDTGML
                     RQKAKPVHEITVEFDGKIDAKWLQSIPTVSKVIHHSENTWKVNYSGDDDVRKAVFTMA
                     VSHNVAVLSMQKEESRLEDVFQLLTQG"
     gene            1968..2513
                     /locus_tag="PKX59_08805"
     CDS             1968..2513
                     /locus_tag="PKX59_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013668173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HOZ91004.1"
                     /translation="MTKNSSEITIIPQEVIINKIYFLRNEKVMLDRDLAELYGVKAIR
                     LREQVKRNLTKFPSHFMFQLTEQEVEIMVSQNAIPSKQHLGGTFPYAFTEHGVLMLAN
                     VLKSERAIQVSIKIIELFVKLREMLVTHTELKLEIERIKKKMDNHDKNIEIVFRYFDE
                     LMEQKINPRKKIGFKLPRKKK"
     gene            complement(2612..3895)
                     /gene="tyrS"
                     /locus_tag="PKX59_08810"
     CDS             complement(2612..3895)
                     /gene="tyrS"
                     /locus_tag="PKX59_08810"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOZ91005.1"
                     /translation="MGFIDELRWRGMLQDIMPGTEELLNKETVTGYIGFDPTSDSLGV
                     GNLVQVTTLMHFQRAGHKPIALVGGATGMVGDPSGKSAERNMLNEETLQHNLNCQKKQ
                     LEKFLDFDCGKNSAEIVNNYDWFRDVKFLDFIRDVGKHITVNYMLAKDSVKNRLENGM
                     TFMEMAYQLVQGYDFYHLYYAKNCKLQMGGSDQWGNIVTGTELLRRKGRVEDAFALTT
                     PLIKKADGTKFGKTESGNLWLDRNKTSPYAFYQFWLNASDEDAKNYIRIFSLKNQQEI
                     EAIESEHLNAPHLRLLQKTLANEITTRVHSQQDCDRAIEISEVFFNGTDKELAQLTES
                     DLQDVFNGLGKNAFEVELQKIQAGIQALDLFAAETTIFTSKGEARKMITANGVSLNKL
                     KFSDPSQIINKEQLIANKFLLIQKGKKNFFLIKAV"
     gene            4018..4260
                     /locus_tag="PKX59_08815"
     CDS             4018..4260
                     /locus_tag="PKX59_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91006.1"
                     /translation="MAHKQIIIPERFLNYLYHAFKNISNPENHRVKLITRDGQKYRNL
                     QVINGCVLLIERSAPVTSNDIEILQLDMDFVPVKVK"
     gene            complement(4325..6460)
                     /locus_tag="PKX59_08820"
     CDS             complement(4325..6460)
                     /locus_tag="PKX59_08820"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOZ91007.1"
                     /translation="MRHLSHIPVLRILIPFVAGIIVGIFMAVPASLAIALLSSGFIGV
                     LVFHFSKYFSQHYKLRYLFGIVLHLFLFSSGVLLTQLKTKLYDPYHYSQFESAEAFLL
                     RITEPYHIKQKSLKAEAEIEAVFENNKWQPCRGKTLLYFASDAAAKALQYADRIVTNA
                     KPQPIAPPQNPGSFNYKRFLGFHQIYHQFYLKSGRWAAAGKGFSNILLSTAFDLRKKF
                     LNEIQGQILVPQEVAVCSALLIGFNDLLDDDLLSAYASSGALHVLSVSGLHVGLIFVT
                     LQRLIFIPENKKYLNLFKQLLILVLIWFYAMLTGLSPSVLRSATMISIIIIAKQLGRD
                     NYMLNTTLASALLLLLINPFSITEVGFQLSYLAVIGIVYLHQRLVTVLYPTSMLMNQA
                     WQLTSVSLCAQLVTFPLGILYFNQFPNLFLVSNLLVIPISTGIIWVSVGATIISALPY
                     MSSLVFYSWKITFALTWLMNQLLLFFENLPFALISGLYIDIAETWIIYGIIAFVVWFL
                     LYNYRNALYGALTLTIVFLLAQLWHEWNILQQKNMTVYALKNSTAIDLAQARKRLVIA
                     PQQIIEDKSLLRFNINRYWYETGKREMLHIPFYQQPAENKLKEIFNSKIVGNVATIQD
                     GFIQMDSKLFFIPGNWLPRKTPLSKIKVDGLIVTSRFRNKLPTLFKYFDSDLLIIDLS
                     SRTKVDAGKLPVSLQIWDCRKQGAAQVEI"
     gene            complement(6594..6824)
                     /locus_tag="PKX59_08825"
     CDS             complement(6594..6824)
                     /locus_tag="PKX59_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91008.1"
                     /translation="MLSKFIAAIAQTTEQQYMERYWNYRDRYKKWFVKIGREAGESIN
                     LTENDPKGGGGALWTSQPLKVDSFLTGFNCSK"
     gene            complement(6901..7563)
                     /locus_tag="PKX59_08830"
     CDS             complement(6901..7563)
                     /locus_tag="PKX59_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91009.1"
                     /translation="MKLHACFYLLAAVIALIYGCKKDEPDEPNVSYEDIKPVKDYVYF
                     QLGTYWIYEDNYGNVDSMYVYSAGEGIDTVRKSNGDIEGIYRFFQSKIHSVYDDYNYY
                     IGYNSGWTSFHPTRHQVFMTKTKPGDYVGQIILMEYPPVIGNILYGEKNSIVKIENYY
                     QIYNIGLVNYVSVIKMSNSIDIINNDQRTNYLIAKNVGIIQEELVDSNNVWKVIRYNI
                     VQ"
     gene            complement(7777..8439)
                     /locus_tag="PKX59_08835"
     CDS             complement(7777..8439)
                     /locus_tag="PKX59_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91010.1"
                     /translation="MKLHACFYLLAAVIALIYGCKKDEPDEPNVSYEDIKPVKDYVYF
                     QLGTYWIYEDNYGNVDSMYVYSAGEGIDTVRKSNGDIEGIYRFFQSKIHSVYDDYNYY
                     IGYNSGWTSFHPTQHKVFMTKTKPGDYVGQIFLTLYPFKIGDWRYWNDSKTTTIDYFQ
                     NYGIDNNSFVSVGKVNVFNDITNNYRQTTNYYIAKNVGIIQEELIDSSNVWKLVRYNI
                     VQ"
BASE COUNT         2911 a         1596 c         1467 g         2797 t
ORIGIN      
        1 ccgatggaag tgtttaccat cttaatcttc ttcccgaaga tatttctgat gtagttatca
       61 atgttggcga ccccgatcgc gtatcaaaag tgagtgcctt ttttgacagc attgaagtaa
      121 aaaagcaaaa acgagagttt gtaacccata ccggtcatta taagggccga agaatatctg
      181 ttatttcaac aggtattggc acagataata tagacattgt ctataatgag cttgatgctt
      241 tagtgaatat tgatcttaaa acaagaactg taaaatccaa tttaacagca cttaatctta
      301 ttcgtattgg cacttccggt gcccttcaac cagaaattcc tgttgattcc tttgtgtttt
      361 cgacattcgg tttaggatta gatggtttgc ttaactttta tcaatacaca aacaccccag
      421 aagagcaggc tataattgat agtttcagaa aacattatcc aaataactgc aaactaccgg
      481 aatcatatct gacacgaagc tcacataaat tagaacaagc actttcagaa ggtatgcata
      541 aaggcattac tgcatcttgt tcaggttttt atgcaccaca aggaagagtg ttacgctatc
      601 aattagcaag aactgatatg attgaaaaat taaattcatt ccgccatgat gagcataagg
      661 ttactaattt tgaaatggaa acaggagcca tgtatggatt ggcaaaatta ttaggacatc
      721 attgctgtgc cgtaaatctg attgtggcaa acagaattgc gcaacagttt tcaaaaaaat
      781 atgaggagcg tatgcatcaa ttaattcaaa ctgtgttgga cagaattagt gctcaacttt
      841 aaatcttcca gagtatgtct gtttcagtaa aaaatgtaac aaaactttat ggcaagcaac
      901 gtgcattgga taatgtaagt tttgaaatca atacaggtca ggttgttggt ttgcttgggc
      961 caaatggcgc aggtaaatca acgcttatga aaattatttc ctgttttatt ccgcagaatg
     1021 aagggaaggt ttctgtttgc ggttttgata cactttcaca atcacttgag gttagacgaa
     1081 atgttggata ccttccagag cataatccac tttatcctga catgtttgtt aaagagtatc
     1141 tacagtttgt gggtggaatt tatttgcctg caaaagaagc tgcaaagcgt gccgactcta
     1201 tgattgccct tacaggttta acaccggaat ataaaaagaa aattggcgca ctctcaaaag
     1261 gctacagaca aagagttggc attgcacaag ctttaattca cgatcctgca gtactcattc
     1321 ttgatgaacc aacttccggg ttagacccca atcagttggt agaaattcgt gcattgattc
     1381 aatcaatagg aaaacaaaaa accgttattc tttctacaca cattatgcag gaagttgaag
     1441 caatatgtaa tcgtgtaatc ataatcaaca aaggaaaaat tgtggctgat gatgatacag
     1501 gtatgcttcg tcaaaaagca aagccagtac atgaaatcac tgttgagttt gatggaaaaa
     1561 ttgatgccaa atggttgcaa tctattccta cggtgagtaa ggttattcat cattccgaaa
     1621 atacatggaa ggttaattac agcggtgatg atgatgtgcg taaagctgtt ttcactatgg
     1681 cagtcagtca taatgtagct gtgctgtcaa tgcaaaaaga agaaagcagg cttgaagatg
     1741 tgtttcaact gctaacgcaa gggtaattta agaatttaca aaaccaatgt tacatcagta
     1801 tctttttatt aatgtcttgt tgtgatattg atatacttaa ttctgaaatt ttgtaaaaca
     1861 tgttataaat tttgtaatac ctatacacag attctgaagg aagaagtaat gtgatttttt
     1921 ataagtactt gttaaacacc taaatttaca tctctgataa tacaagaatg acaaaaaata
     1981 gttctgaaat aacaataata ccacaggaag taataataaa taaaatttat tttttaagaa
     2041 atgaaaaagt gatgcttgac agagatttag ctgaattata tggtgttaaa gccatcagac
     2101 tacgagaaca agtaaaacga aatctaacaa aatttccttc ccattttatg tttcagttaa
     2161 ctgaacaaga ggttgaaatt atggtatcgc aaaatgcgat accttcaaaa caacatttag
     2221 gtggtacttt tccttatgca tttacagaac atggtgtact aatgttagct aatgtcttaa
     2281 agagtgagag agctatacag gtaagtatta aaataattga gttgtttgtg aaacttcgtg
     2341 aaatgttggt tacacatact gaactgaagt tagaaataga acggataaaa aagaaaatgg
     2401 ataatcatga taagaatatt gaaattgtgt ttagatattt tgatgaattg atggaacaaa
     2461 aaataaatcc cagaaagaaa ataggattta aactaccaag gaaaaagaaa taaccacata
     2521 taaatcctgc ctgttaaaac caaataacag ataatttaaa aagattgttg ctaaatgctt
     2581 agaaaagtaa caaatactta tgctaagaag tttaaacagc cttaatcagg aaaaaattct
     2641 tcttaccttt ctgaatcagt aaaaacttat ttgcaatcag ttgttcttta ttgataattt
     2701 gtgaagggtc agaaaatttc aatttattca atgagacacc atttgcagta atcatttttc
     2761 ttgcttcacc tttacttgta aaaattgttg tctctgctgc aaataaatca agtgcctgaa
     2821 tacctgcctg aattttttgc aactcaactt caaatgcatt tttgcctaat ccgttaaaaa
     2881 catcctgcaa atcagactct gttagttgcg caagttcttt atctgttcca ttaaaaaata
     2941 cttcagaaat ttctatagct ctgtcacaat cctgttgtga atgcacacga gtagttattt
     3001 cattagctaa tgttttctga agcaatctca aatgaggtgc atttaaatgt tcagactcta
     3061 ttgcttctat ctcctgctga ttctttaaag aaaatatacg gatataattt tttgcatctt
     3121 catcgcttgc attcagccag aactgataaa aagcataagg agatgtttta tttctgtcaa
     3181 gccacaaatt tccactctct gtcttaccga atttagtacc atctgctttt tttatcagtg
     3241 gtgttgttaa agcaaatgca tcttcaacac ggccttttct gcgaagcaat tcagttcctg
     3301 taacaatatt tccccactga tcgctgccac ccatttgcag cttacagttt tttgcataat
     3361 acaaatgata aaaatcgtag ccctgcacta actgatatgc catttccata aatgtcattc
     3421 cattctctaa tctgtttttt acagaatctt ttgccagcat ataattaaca gtaatgtgtt
     3481 tccccacatc acgaataaaa tcaagaaact ttacatccct gaaccaatca taattattaa
     3541 ctatttctgc tgaatttttt ccacaatcaa aatccagaaa tttttctaat tgttttttct
     3601 gacaatttaa attgtgctgc agtgtttctt cgtttaacat atttctttca gcactctttc
     3661 cgcttggatc gccaaccata cctgttgcac cgccaaccaa ggcaattggt ttatgtcctg
     3721 ctctttgaaa atgcatcaat gtagtaacct gcactaaatt gcctacgcct aatgaatcgc
     3781 tggtaggatc aaatcctata tatccggtaa ccgtttcttt attcaataat tcctctgtac
     3841 cgggcataat atcctgcaac atgccgcgcc atcttagttc gtcaataaat cccatatctg
     3901 atttttagtg ccggcaaaga taatcataca gtgctttact gctatcagtt acaaagggta
     3961 aaattcatgc aaccttttta tctgtatcac cgtacaacac gcttaaacat tatcaatatg
     4021 gcacataagc aaatcattat ccccgaaaga tttctaaact atttatatca tgcttttaaa
     4081 aatataagca atcctgaaaa tcacagagta aaacttatta ctcgtgacgg acagaagtat
     4141 cgaaatctgc aggttattaa tggctgtgta ttgttaattg aaagatctgc acctgttacc
     4201 tcaaacgata ttgagattct tcaacttgat atggattttg ttcctgtaaa agttaaataa
     4261 tatttgatgt tggcacagcc ggtagttcct tttggggatt gccggttttt tatttgctgc
     4321 tccctcaaat ttctacttgt gctgcgcctt gttttctgca atcccagatt tgtaaactga
     4381 caggcaattt acctgcatca acttttgttc ggctgcttaa atcaataatc aacaaatcag
     4441 aatcaaaata tttaaagagc gtaggcagtt tatttctgaa acgtgaggta acaatcaatc
     4501 catcaacttt aattttactt aatggtgttt ttcgcggcag ccagttaccc ggaataaaaa
     4561 ataatttact atccatctga ataaatccat cctgaatagt agcaacattt cctacaatct
     4621 tactgttaaa aatttctttc aacttatttt ctgcaggctg ctgataaaat gggatgtgaa
     4681 gcatttctct ttttcctgtt tcataccaat agcggttaat attaaaacgt aacaggcttt
     4741 tatcttcgat tatctgttgt ggtgctatga ccaacctctt tcttgcctgt gccagatcaa
     4801 ttgctgtgct gttttttaaa gcatatactg tcatgttttt ctgttgcaga atattccact
     4861 catgccaaag ttgagccaac agaaaaacta ttgttaacgt caatgctcca taaagtgcat
     4921 tcctgtagtt gtaaagcaaa aaccaaacta caaaagcgat aatgccatag ataatccatg
     4981 tttctgcaat atcaatatac aatccgctaa tcaatgcaaa tggaaggttt tcaaaaaaca
     5041 acaataactg attcattaac caagtgagtg caaaagttat tttccaagaa taaaatacaa
     5101 gtgatgacat gtagggcaat gctgatataa ttgtagcacc aacagacacc cagataattc
     5161 ctgtagagat aggtatgact aaaagattag atactaaaaa caaattcggg aactgattaa
     5221 aataaagtat tcctaacgga aatgttacca gctgcgcaca taatgacact gaagtcagtt
     5281 gccatgcctg attcatgagc attgaagtag gataaagaac cgtaaccaat cgctgatgca
     5341 gatatacaat tccgataaca gcaaggtatg aaagttgaaa accaacttct gtaattgaaa
     5401 atgggttaat caacaacaac aataaagcag aagccagtgt agtgttaagc atgtagttat
     5461 cacgaccaag ttgctttgca ataatgatga tagaaatcat ggttgctgac cgtaaaacgg
     5521 aaggagaaag tcctgttagc atggcataaa accatatcaa aaccagaatg agtaattgtt
     5581 taaaaagatt cagatacttc ttattttcag gaataaaaat cagcctttgc agggtcacaa
     5641 aaatcaagcc tacatgcaat ccggatacac tcaacacatg taatgcaccg gagcttgcat
     5701 aggccgacaa taaatcatcg tcaagcaaat cattaaagcc aattaacaat gcagaacaga
     5761 ccgctacttc ctgcggcact aaaatttgtc cttgtatttc attcaaaaac tttttgcgta
     5821 aatcaaaagc tgtactcaaa agaatattag aaaatccttt accggcagca gcccatcgcc
     5881 ccgactttaa atagaactga tggtatatct gatgaaaacc aagaaatctt ttgtagttaa
     5941 atgaccccgg attttgtgga ggagcaattg gctgtggctt tgcattggta acaatcctat
     6001 cagcatactg caatgccttt gctgctgcat cggatgcaaa atacaacaat gtttttcctc
     6061 tgcatggctg ccatttattg ttttcaaaga cagcttctat ttctgcttct gcttttaaag
     6121 atttttgttt gatgtgatat ggctcggtga tacgaagtaa aaaagcttca gcgctttcga
     6181 attgcgaata gtgatagggg tcgtaaagtt tcgtctttaa ttgtgtgagt aaaacgcctg
     6241 aagagaataa aaacaaatgc aatactattc cgaaaaggta gcgaagttta taatgctgac
     6301 taaaatattt tgagaaatga aaaactaaaa ctccgataaa acctgaagac aacaaagcga
     6361 ttgccaaaga tgcaggcaca gccatgaata tgcctacaat tataccggca acaaaaggta
     6421 ttaaaattct taaaacagga atatgcgata ggtgacgcat aactgtgcgg tttggtgtaa
     6481 aattaagaaa attaattctg aattgtagaa tttaatcgag aataatttat cttacttaag
     6541 gctttttaca gcagggcctg cttccgttta aattcatcct gcaatattct atatcattta
     6601 ctacaattaa aacctgtcaa aaagctgtcc accttcaaag gctggcttgt ccacaatgcg
     6661 ccacctcctc ccttggggtc gttttcggtc aggttaatgc tttcgcccgc ctcacgccct
     6721 atttttacaa accatttctt gtatcggtct ctgtagttcc agtagcgttc catgtattgc
     6781 tgctctgttg tttgtgcaat agcggcaatg aattttgaaa gcacagcagc agcatggcta
     6841 tggttttcca gttgatttta attttcatac tatttggttt ttagtggtta ttaataaatt
     6901 ttattgtact atgttatacc ttattacctt ccatacatta ttgctatcaa ccagttcttc
     6961 ctgaataata cctacgtttt tggcaattaa ataatttgtc ctctgatcat tattaattat
     7021 atctattgaa ttcgacattt taataacaga tacataattt actaacccaa tattatatat
     7081 ttgataatag ttttcaattt ttacaataga atttttctca ccatataata tattaccaat
     7141 gacaggagga tattccatta aaattatttg ccctacataa tctccgggct ttgttttagt
     7201 cataaagact tgatgtctgg ttggatgaaa agatgtccag cctgagttat aaccaatata
     7261 ataattgtaa tcatcataaa cactgtggat tttactttga aaaaagcggt aaataccttc
     7321 aatatcacca tttgattttc ttacggtatc aatgccttct ccggcagaat atacatacat
     7381 gctgtcaacg tttccgtaat tatcctcata tatccagtaa gtgccaagct ggaaataaac
     7441 ataatcctta acaggcttga tgtcttcata gcttacattt ggctcatcag gctcatcttt
     7501 tttgcagccg taaatcaaag caataacagc agcaagcaaa taaaaacagg catgcagctt
     7561 catgagtgtt ttcttgaagg tgtaaatata tgaggcttga gttaaaacaa atatttttaa
     7621 aaattattta tgcgtatgct ttgcacaaac agcggcaatg aattttgaac gcacagcaac
     7681 agcatggcta tggttttcca gttgatttta attttcatac tatttggttt ttagtggtta
     7741 aatgaatcta tcaatagtaa atattaaaga ttaggtttac tgaacaatat tatatctgac
     7801 taacttccac acattgctgc tgtctatcag ttcctcttgt atgattccta cattctttgc
     7861 aatataataa tttgttgttt gtctataatt attcgtaata tcattaaaaa cattaacctt
     7921 tccaaccgaa acaaaactat tattgtcaat accataattt tggaaataat caatagtagt
     7981 tgttttagaa tcattccagt atctccaatc acctatttta aagggatata aggttaaaaa
     8041 aatttgccct acataatccc ccggctttgt tttagtcata aaaaccttat gttgggttgg
     8101 atgaaaagat gtccagcctg agttataacc aatataataa ttgtaatcat cataaacact
     8161 gtggatttta ctttgaaaaa agcggtaaat accttcaata tcaccatttg attttcttac
     8221 ggtatcaatg ccttctccgg cagaatatac atacatgctg tcaacgtttc cgtaattatc
     8281 ctcatatatc cagtaagtgc caagctggaa ataaacataa tccttaacag gcttgatgtc
     8341 ttcatagctt acatttggct catcaggctc atcttttttg cagccgtaaa tcaaagcaat
     8401 aacagcagca agcaaataaa aacaggcatg cagcttcatg agtgttttct tgaaggtgta
     8461 aatatatgag gcttgagtta aaacaaatat ttttaaaaat tatttatgcg tatgctttgc
     8521 acaaacagca tccctggcgc aagtcttcgg acttgtgccc acctatcaat aaagaatgca
     8581 atgcaataat ttagcgcaac aaggcagcaa gcaggttaaa ccgcacttta ctttgctcaa
     8641 ataatattta caatcaatag aagttctccc tggcgcaagt cttcggactt gtgcccacct
     8701 gtcaataaag aatgctatgc aataatttag cgcaacaagg cagcaagcag gttaaaccgc
     8761 actttactct g
//
LOCUS       DAOOOI010000078         8555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1627_length_8555_cov_16.844353, whole genome shotgun sequence.
ACCESSION   DAOOOI010000078 DAOOOI010000000
VERSION     DAOOOI010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1627_length_8555_cov_16.844353"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PKX59_08840"
     CDS             <1..1029
                     /locus_tag="PKX59_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOZ91011.1"
                     /translation="PYGNDVYYQLRNTIAIPQSEIIKLNDMQGLNPTLAPLKEIYDQG
                     WMSIINNVGYPNPNRSHFRSMDIWQTASDSNQFLSTGWIGRYLDSNCQMCKYPYNAIE
                     VDDTLSLTLKGDSKKGIALQDPDKLFRTTNTPFFGKLIEHDKEHLDEDNLGYLYKTMI
                     ETYSSASYIKETTKNYDNNFVYPESSFAKKLKTVARFILSGLDTRVYYVSLSGFDTHV
                     NQMAQQKKLFTQFSEGMNAFVKELNNNKKLEDTLVIAFSEFGRRVAQNASRGTDHGTA
                     NVMMLFGGQLKKQGIYNDAPDLNDLDAGDLKYKVDFREIYATVLNKWLNVDNKLILPD
                     STGTMDFI"
     gene            complement(1047..2441)
                     /locus_tag="PKX59_08845"
     CDS             complement(1047..2441)
                     /locus_tag="PKX59_08845"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOZ91012.1"
                     /translation="MKNKYSYVLNSIFKIIVILLLPNSINAQFGSPFVIDSANNTLVN
                     TINIVDLDNDNQKDILVSNLYDSVYWYKNNGLLFNRMSAISNTVKRPYFVDCADVDGN
                     GLQDVLVSYDDVYAGLKLFKNFGGNTWQQIIINDSIDAFVVRSYLVDLDNDSDLDIIS
                     CQDLEVAIYEQSAGTFSGMIKVADSSEFYNLVVHDFNGDGFVDFSVHSAAGFRLFINN
                     HNLTFSPQTFTTALYLTLESCDIDNDGDFDLFYPDSAVSFNLITLKNDGSGGFSNFQS
                     VMYHAGNNQNPPLKFLTLDNDTFPDALYIPLVNRGLYYKSNDGVGGLNFSHFIDSVYR
                     YTFVNGGDLDGDNDNDLVWFANEPFGIKRVGYTLNNSGNTDYNELSSQEFYFYPNPVS
                     NNILNIKYFSPTSSGGVFEIVDAIGKTVFKYTLPLYSSNHQITLPQLQNGVYNAMLTY
                     EGAKICRKLAIVDN"
     gene            complement(2619..3593)
                     /locus_tag="PKX59_08850"
     CDS             complement(2619..3593)
                     /locus_tag="PKX59_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOZ91013.1"
                     /translation="MESIDFLQREIERELKLLRFGHEPANLYEPMEYIVELGGKRMRP
                     LLVLIGAGLFETNYQKAMKAAIGIELFHNFTLLHDDIMDKATLRRNKPTVHEKWSADI
                     AILSGDALFVRASEYIMQVDDAILRPVMQLFYKTAMQVCEGQQYDMDFGRLREITPED
                     YIGMITLKTAVLPACCLATGAIIANASEEDKLRLYEFGKNIGIAFQIQDDLLDAFGDD
                     TKFGKRPGGDIIEGKKTFLFSEACRLLNGKEHLDFLTLYNSHNLSNHEKITEVMQVFN
                     NLELHHHTMRTIQSYYQKGLEQLAGLNVQAKNVLELKLFCEKLLIREH"
     gene            complement(3603..4547)
                     /locus_tag="PKX59_08855"
     CDS             complement(3603..4547)
                     /locus_tag="PKX59_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOZ91014.1"
                     /translation="MKSKILVTGGAGFVGSALAEKLADNADNFVVVVDNLLTGSMRKV
                     PASKHNNVKFIKADVNDFSDISSIFYAYAFDYVFHYAAVVGVKRTLDHPSWVLKDLTG
                     LKNILDLSKNTGVKRVYFSSSSEVYGEPVEYPQNETTTPLNSRLPYAIVKNLGEAYLR
                     AYKQEFDLDFTIFRFFNTYGPKQSTDFVMSKFIEAALSDKDITIYGDGSQTRTFCYVD
                     DNVEACLNAFYTHRFVNDVINVGNDHETSILELAQLVKKILNSKSNIVHLPALKEGDM
                     TRRCPDIGKMKILLNRELTPLETGMERVIEVFRNKKKK"
     gene            complement(4562..5431)
                     /gene="rfbA"
                     /locus_tag="PKX59_08860"
     CDS             complement(4562..5431)
                     /gene="rfbA"
                     /locus_tag="PKX59_08860"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOZ91015.1"
                     /translation="MKGIVLAGGSGTRLHPLTLAMSKQLMPVYDKPMIYYPLSVLMMA
                     GIREILIISTPHDLPHFKKLLGSGEQIGCRFEYAEQAVPNGLAQAFVIGEKFIGSDSA
                     ALVLGDNIFYGQGLGRILADNNKINGGLVFAYHVSDPERYGVVEFDGNYKALSIEEKP
                     QKPKSNFAVPGLYFYDNTVIEVAKNLKPSARGEYEITDVNKHYLQQGKLKVQLLERGT
                     AWLDTGTFNSLLQAGNFVNVIEERQGLKIGCIEEIAYRMKFIDKEQLRKVAEPLTKSG
                     YGNYLMKIIDQNL"
     gene            5734..6021
                     /locus_tag="PKX59_08865"
     CDS             5734..6021
                     /locus_tag="PKX59_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91016.1"
                     /translation="MKINPTGHFTGFDLSVSPANVVDINSSSSAISGLRLRNLSTTSN
                     SNGKVLSVDNSGDVILVRDNSVELIAMIKQLQQQVNSLQQKLEVTLNQHNN"
     gene            6059..6232
                     /locus_tag="PKX59_08870"
     CDS             6059..6232
                     /locus_tag="PKX59_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91017.1"
                     /translation="MLIFYNTDGSILKMVKIHQTGEGSLLVYGNNLSSGQYSYSLVVN
                     NKTIKTKQMVKVK"
     gene            6386..6526
                     /locus_tag="PKX59_08875"
     CDS             6386..6526
                     /locus_tag="PKX59_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91018.1"
                     /translation="MKKSIAKKTRKAKKLIAKPAAIVAFLRDLFAEELEVLNRKNIAM
                     HN"
     gene            complement(6537..6974)
                     /locus_tag="PKX59_08880"
     CDS             complement(6537..6974)
                     /locus_tag="PKX59_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOZ91019.1"
                     /translation="MLNKARRYCTYQERSQQELREKLYTLGLFKKDVEETIAMMVTEG
                     YLNEERFAIAYAGGKFRIKHWGKNKIKQALKLKKVSDYCIRQALKSIADDDYLKVLNE
                     QTEIYKKKIKERNPLKKNYKVAQQLMARGFEADEVWKCILKEK"
     gene            complement(6992..8419)
                     /gene="pyk"
                     /locus_tag="PKX59_08885"
     CDS             complement(6992..8419)
                     /gene="pyk"
                     /locus_tag="PKX59_08885"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOZ91020.1"
                     /translation="MQHFNKTKIITTLGPATNSKEAIYKLIQAGADVMRINASHGDHT
                     FLKSIVNNVREINEEKQLNIALLLDLQGPKLRIGDIEGGQCELADKQQFVLTTKEMEG
                     NATKATIRYKDFASDVKKGDIILIDDGKLKLEVKQITAEQDVVTQVINGGVLMSKKGV
                     NLPFTNISIPSITEKDRADIDFAIENNIEWIGLSFTRTAQDILNLKKIIKDCGNAMRV
                     IAKIEKPEAVRNINEIIEVTDGVMVARGDLGVEIDLAQVPIIQKKIVKKCRAAGIPVI
                     IATQMMESMITNFRPTRAEANDVGNAVYDGADALMLSAETSVGKFPVETVNSMRKIIQ
                     AIEHDQEIYYRMNQPDKNSKTFISDTLCYHAAELANQSGAKAIAAMTHSGYTAFRISS
                     QRPKAGLFIFTDHKPILNTLNLVWGVRGMYYNKFESTDQTIDDIKNILKEQGYVGAGD
                     VVIHVASTPLKKRSRVNTLKINVVD"
BASE COUNT         2705 a         1628 c         1437 g         2785 t
ORIGIN      
        1 ccttatggta atgatgtata ttatcagttg cgaaacacta ttgccattcc acaatcggaa
       61 atcattaaat taaatgatat gcagggattg aatcctacat tagctccact aaaagaaatt
      121 tacgatcagg gttggatgag tatcatcaat aatgtgggtt atccgaatcc aaaccgctct
      181 cactttaggt caatggacat ctggcaaaca gcaagtgatt ccaatcagtt tctttctaca
      241 ggatggattg gacgctattt agattcaaac tgtcagatgt gcaagtatcc atacaatgct
      301 attgaagtgg acgacacctt atctttaact ttgaaaggtg attctaaaaa aggtattgca
      361 cttcaggatc cggacaaatt gtttcgtacc accaacacac ctttttttgg caagttgata
      421 gaacacgata aagagcatct tgacgaagat aatctaggat acttgtacaa aaccatgatt
      481 gaaacatatt catcggcaag ctatattaaa gaaacaacaa agaattatga taataacttt
      541 gtgtatcccg aatcgtcatt tgcaaagaaa ttaaaaaccg ttgcgcgatt tatattatca
      601 ggacttgata caagagttta ttatgtttct ctcagtggat ttgatacgca tgtaaaccaa
      661 atggcgcaac aaaaaaaatt gtttactcag ttttctgaag gcatgaatgc atttgtaaaa
      721 gagttgaaca ataataaaaa acttgaagat acattagtga ttgccttttc ggaatttggt
      781 agaagagtgg cgcaaaatgc aagccgtggc accgaccatg gtacagcaaa tgttatgatg
      841 ctgtttggcg gtcagttaaa gaagcaggga atatataatg atgctcctga tttgaatgat
      901 cttgatgcag gagatttaaa atataaagtt gatttcagag agatatatgc aacagtattg
      961 aacaaatggc tcaatgtgga caataaatta attcttcctg attcaacagg tacaatggat
     1021 tttatttgac gcaagataca atgagattag ttatcaacta tagctaattt cctgcaaatt
     1081 tttgctcctt cataagttaa catagcattg taaactccat tttgtaattg tggtaatgtg
     1141 atttgatgat tgctgctata caatggcaat gtatatttaa aaacagtttt tccaattgca
     1201 tcaacaattt caaaaacacc acctgaagaa gttggtgaaa aatatttaat gtttagaata
     1261 ttattactta caggattggg ataaaaataa aattcctgag atgacaactc attataatca
     1321 gtgttgccgg aattgttcag ggtatatcct actcgtttaa tgccgaaggg ttcattggca
     1381 aaccatacaa ggtcattatc attatcgcca tcaagatcac caccattaac aaaagtataa
     1441 cgataaacag aatcaataaa atgagagaag tttaatccac ctactccatc atttgacttg
     1501 taatagaggc ctctgtttac aagtggaata taaagtgcat ccggaaatgt atcattatct
     1561 aaagtgagaa attttaatgg tggattttga ttgttacctg catggtacat cacggattga
     1621 aaattactaa aacctcccga gccatcattt ttcaaagtta tcaagttgaa agaaacagct
     1681 gaatcgggat agaaaagatc aaagtcacca tcattatcaa tgtcacaaga ttctaaagtg
     1741 agataaagtg cagttgtaaa tgtttgagga ctgaaagtaa ggttatgatt atttataaaa
     1801 agcctgaatc cagcagcaga atgaactgaa aaatcaacaa agccatcacc attaaaatca
     1861 tgcacaacta aattgtaaaa ttcactactg tctgctactt taatcattcc tgaaaaagtt
     1921 cctgcagatt gctcataaat tgcaacctct aaatcctgac aactgataat gtcaagatct
     1981 gaatcgttat ctaagtcaac taaataactt cttactacga aagcatcaat actatcattg
     2041 atgattattt gctgccaggt gtttccaccg aaatttttaa ataacttcaa tcctgcatag
     2101 acatcatcat aactcaccaa aacatcttgt aatccgttac catcaacatc tgcacaatct
     2161 acaaaatatg gacgtttcac tgtgtttgaa attgcagaca tacgattaaa taaaagtcca
     2221 ttgtttttat accaataaac agaatcatat aagtttgata ctaaaatatc cttttggtta
     2281 tcattatcaa ggtcaacaat attgattgta ttgactaaag tgttgttggc tgaatcaata
     2341 acgaatggtg aaccaaattg agcattgata gaatttggta ataacagaat aacaatgatc
     2401 ttgaaaatag aattcaacac ataagagtat ttgtttttca tactgtgacg ttctaatcag
     2461 atactaaaac tgtttcaaat tgaagtgccc tttatagaat gtgtaaaaat aaatttttcc
     2521 ttatacgaac ttcataagga ttgtgtaaaa tagttgtgat tcacaaaaac gtaaatgtaa
     2581 ggtttaaatt aatttccaga aaaggttatt acacagtttc aatgctccct tatcaacagc
     2641 ttctcacaaa ataattttaa ttcaagaaca ttttttgcct gcacatttaa gcccgcaagt
     2701 tgctccaacc ctttttgata ataggattga attgtccgca tggtatgatg atgcaattct
     2761 aagttattga acacctgcat cacttcagta attttttcgt gattggataa attgtgtgag
     2821 ttatacagtg tcagaaaatc aagatgctct ttgccgttta acagcctgca tgcttctgaa
     2881 aaaagaaatg tttttttacc ctcaataata tcgccaccgg gacgtttacc aaatttagta
     2941 tcatcaccaa aagcatctaa caaatcatcc tgaatctgaa atgcaatacc tatatttttt
     3001 ccaaactcat acaatcgtaa tttgtcttct tctgaggcat tggcaattat tgcacctgtt
     3061 gccagacaac atgcaggtag caccgctgtt tttaaagtaa tcatgccaat ataatcttca
     3121 ggagtaattt cgcgcaatcg cccaaagtcc atgtcgtact gttgtccttc gcaaacctgc
     3181 atagctgttt tataaaacaa ctgcatcacc ggtcttagta tggcatcatc aacctgcata
     3241 atgtattcac tggctcttac aaaaagcgca tcaccggaaa gtattgcaat atcggcactc
     3301 catttttcat gcaccgttgg tttgtttctt ctcaaagttg ccttatccat gatgtcatca
     3361 tgcagcagtg taaaattgtg aaacaactca atgcctatgg cagctttcat tgctttctga
     3421 taatttgttt caaacaagcc tgctcctata agaaccaata atggccgcat gcgtttgcca
     3481 ccaagctcaa ctatatattc cataggctca tatagattgg ccggctcatg accaaagcgc
     3541 agcaacttta attcgcgttc aatttcgcgt tgcagaaagt caatactttc catcaaaaaa
     3601 aattattttt tctttttatt tctgaaaacc tcaatcactc tttccatgcc tgtttcaaga
     3661 ggcgttaact cgcgattgag taatattttc attttgccaa tatcaggaca tctgcgtgtc
     3721 atatcacctt cttttaaagc aggtaaatgc acaatgttcg attttgaatt cagaattttc
     3781 tttaccaact gcgccaattc cagaatagaa gtttcatggt cattgcccac attgatgacg
     3841 tcattcacaa aacgatgtgt gtagaaggca tttaaacagg cttcaacatt atcatcaaca
     3901 taacagaagg ttcgtgtttg tgaaccatcg ccatagatag taatgtcttt atcgctcaat
     3961 gcagcttcaa taaatttcga cattacaaag tctgtactct gcttaggtcc gtatgtgttg
     4021 aagaatcgga aaatggtaaa gtcaagatca aactcctgct tgtaggcacg cagataggct
     4081 tcgccaagat tttttacaat ggcataagga agccttgagt ttaaaggtgt tgttgtttca
     4141 ttttgtggat actcaacagg ctcaccataa acttcgctgc tgctgctgaa gtaaacacgt
     4201 tttactcctg tgttctttga taagtcaaga atatttttca atcctgtaag atcttttaat
     4261 acccatgatg gatgatcgag tgtgcgtttt acaccaacta cagcagcata atgaaaaaca
     4321 tagtcaaatg cataggcata aaaaatggaa gatatatcgc tgaaatcatt tacatctgct
     4381 ttaataaatt taacattgtt atgtttcgat gccggaacct ttcgcatgga acctgtaagc
     4441 agattatcaa ctacaacaac aaaattgtca gcattgtccg ctaatttttc tgccagggca
     4501 cttcctacaa atcctgctcc accggttact aaaattttac ttttcatata ttttgtttcg
     4561 cttataaatt ttgatctatt attttcatca gataatttcc atagccgctt tttgttaatg
     4621 gctctgctac tttccgcaat tgttctttat caataaattt catacggtag gctatttctt
     4681 ctatacaacc tatttttaaa ccttgtcttt cttcaattac attcacaaag tttcctgctt
     4741 gcagtaatga gttaaacgta cctgtatcga gccaggctgt accacgctca agtagctgca
     4801 cttttaattt accttgttgc agatagtgct tgtttacatc ggtaatctca tattcaccac
     4861 gtgcacttgg ctttaaattt tttgcaactt caataacggt gttgtcataa aaatacaatc
     4921 cgggaacagc aaagttcgat ttgggttttt gaggtttctc ttctatggaa agtgctttat
     4981 agttgccatc aaactcaact acaccatatc tttcagggtc tgaaacgtga taggcaaaaa
     5041 ccaatcctcc gtttatttta ttgttgtctg caagaatacg tcctaatccc tgaccataaa
     5101 aaatattatc gccaagaacc aaagctgcac tgtcgcttcc aataaatttt tcaccgataa
     5161 caaatgcttg tgccagtccg ttaggaacag cctgttctgc atattcaaaa cggcaaccaa
     5221 tttgttcgcc actgcctaaa agttttttaa agtgaggcaa gtcatgcggt gtgcttatga
     5281 ttaaaatttc tcttattccg gccatcatca aaacagatag cggataataa atcataggtt
     5341 tatcatacac cggcataagc tgtttgctca tggctaatgt aagtgggtgc agacgtgtgc
     5401 cgctacctcc ggctaaaaca attcccttca tcgtatggtt gcaaaagtgt atttgtacgt
     5461 aaaattacgt caggtggttt ctaaaaattt aaggctgtaa aggtaataat gttataaaat
     5521 aacagaaatg tcaatttgtt gaggtctatg agcctgtttt gaagaaaccc tgttttgaaa
     5581 aaataattac atcacataga attaaaataa ggttttgctg ttggccctct taccaaacta
     5641 caaattttaa gtaaaaatgt accacagttt cgtatttctt atgattatgg aaataatata
     5701 cacaccgact ttcaaacaac agcaagtggt gatttgaaaa tcaatccaac aggacacttt
     5761 acaggatttg atttgtctgt ttcgcctgca aatgttgttg atattaattc ttcctcatca
     5821 gcaattagcg gattgcgttt aagaaattta agtacaacaa gcaattcaaa tggtaaagta
     5881 cttagtgttg acaatagtgg cgatgttatt ttggtgaggg ataacagtgt agaactaatt
     5941 gctatgataa aacaacttca acaacaagta aattcattac aacaaaaatt agaagtaaca
     6001 ttgaatcaac acaacaatta gctggaacat actaccacaa caagaaccat taaacgccat
     6061 gttgattttt tataacaccg atggcagcat tttaaagatg gttaagattc accaaacagg
     6121 agagggttct ctgttggttt atggaaacaa cctttcatca ggtcagtata gttattcttt
     6181 agtggtcaat aataaaacca ttaaaaccaa acaaatggta aaggtgaagt aggggattgt
     6241 ttattattca gttatatgga agttggtttt gttaaactgt ttacttaacc tgagatactg
     6301 aaatacagca ggttatcaat ttataaattt ttggtacaga atttgaaata tggataagta
     6361 agtataacat ttaatactcc atattatgaa aaaatcaatt gctaaaaaaa ccagaaaagc
     6421 aaagaaatta attgcaaaac ccgcagccat tgttgcattt cttcgcgatt tgtttgctga
     6481 agaacttgaa gtccttaacc gcaaaaacat agccatgcat aactaatctg catggcttat
     6541 ttttctttta aaatacattt ccaaacttca tcggcttcaa aaccacgagc cattaattgc
     6601 tgcgcaacct tgtagttttt tttaagcgga ttcctttctt ttattttttt cttgtaaatc
     6661 tctgtttgct cattcaacac tttcaaatag tcatcatcag ctatagattt taatgcctgc
     6721 ctgatacagt agtcagaaac tttttttagt ttcaatgcct gtttgatttt gttttttccc
     6781 cagtgtttta tccggaattt tcctccggca taggcaatag caaagcgttc ttcattaagg
     6841 tagccttctg taaccatcat ggcaatggtt tcttctacat cttttttaaa taatccgagc
     6901 gtgtaaagtt tttcgcgcag ttcctgctga cttctttcct gataggtgca atacctgcgg
     6961 gctttgttca acacatcgct gctaaccatc cttagtccac tacattaatt tttaaagtat
     7021 ttacccgcga acgttttttc agtggcgtac tggcaacatg tatcaccaca tctcctgcac
     7081 caacataacc ttgttctttc aatatgtttt taatatcgtc aatagtctgg tctgtacttt
     7141 caaatttatt gtaatacata cctctcacac cccaaactaa attcaaggtg tttagaatgg
     7201 gtttgtggtc ggtaaaaata aaaagccctg ccttgggtcg ctgactcgaa attctgaatg
     7261 ctgtgtatcc tgaatgtgtc atagctgcaa tagcttttgc acccgactga ttggccaact
     7321 ccgctgcatg ataacacaag gtgtcagaaa taaatgtttt ggaattttta tcaggttgat
     7381 tcatgcggta ataaatttcc tgatcgtgtt caattgcctg aataattttt cgcatagaat
     7441 ttacagtctc aacaggaaat tttcctacag aagtttctgc actcagcatc aaagcatcgg
     7501 caccatcgta aacagcatta cccacatcgt tggcttcggc acgtgtaggt ctgaaattag
     7561 taatcatact ctccatcatc tgtgtggcaa tgattacggg aatgcctgcg gcacgacatt
     7621 tttttacaat ctttttctga ataataggaa cctgtgccag atcaatctct acacctaaat
     7681 caccacgggc caccataaca ccatcagtaa cttcaataat ctcattgata tttctgactg
     7741 cttcgggttt ttcaattttt gcaataaccc tcatggcatt gccgcagtct ttaataattt
     7801 tcttaagatt taatatgtcc tgtgcagttc tggtaaacga taagccaatc cattcaatat
     7861 tgttttcaat ggcaaagtca atgtctgcac ggtctttttc tgttattgag ggaatactga
     7921 tattggtaaa cggaaggtta actcccttct tcgacatcag cacaccacca tttatcacct
     7981 gtgttacaac atcctgttcc gctgtaattt gctttacttc taactttaat tttccgtcat
     8041 caatcagaat aatatcacct ttctttacat cgctggcaaa atctttataa cggatagtag
     8101 cttttgtagc attgccttcc atctcctttg ttgtgagtac aaattgctgc ttgtcggcaa
     8161 gttcgcactg tccgccttca atatcaccga tgcgcaattt aggaccttgc aaatcgagca
     8221 ggagtgcaat attcagttgt ttttcttcat tgatttctcg gacattattt acaattgact
     8281 ttaaaaaagt atggtcgcca tgcgaagcat ttatacgcat aacatcggca cctgcctgaa
     8341 tgagtttgta aatggcttct ttggaattgg tggccggccc aagggtggta ataattttag
     8401 ttttattaaa atgttgcata atcagtcgaa gataaaattg tctgccgact tgagttcttc
     8461 aacacgaagc tcactaatgg cagaaactat ttctatttgt tttattttgt tgcgaatgtc
     8521 attaaagtca aaataggctc cgtaattttt tacaa
//
LOCUS       DAOOOI010000079         8414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1662_length_8414_cov_13.443474, whole genome shotgun sequence.
ACCESSION   DAOOOI010000079 DAOOOI010000000
VERSION     DAOOOI010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1662_length_8414_cov_13.443474"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PKX59_08890"
     CDS             <1..458
                     /locus_tag="PKX59_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ91021.1"
                     /translation="FAHMEEIDLCWRMKHSGHKLYCIPASRVYHVGGGTLHKSNPKKT
                     YLNFRNNLMMLLKNLPSGNRFSVFTKRMYLDKLAFIKFFISGNFKDAWAVYRAYFYVI
                     LHFKTLKKKRVEIKNINTNVSCIFEKSIVAEHFFRGKKKFTELNQDCFS"
     gene            479..1090
                     /locus_tag="PKX59_08895"
     CDS             479..1090
                     /locus_tag="PKX59_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009056655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOZ91022.1"
                     /translation="MKIICVGRNYAAHAAELKNPLHSEPVIFMKPETAQLLPRMPFFI
                     PDFSTDIHHEVELVVKINRTGKHIQEQFANTYYDEIGIGIDFTARDKQSQLKEKGLPW
                     ELAKAFDGSAPTGKFLNKSLFKSVEDINFRLEKNGNIVQQGNSSNMLFNIDKIIAFVS
                     SYITLKKGDLIFTGTPEGVGKVQANDVLDAFIEDQHLLTVKIK"
     gene            1190..2569
                     /gene="mgtE"
                     /locus_tag="PKX59_08900"
     CDS             1190..2569
                     /gene="mgtE"
                     /locus_tag="PKX59_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOZ91023.1"
                     /translation="MLQQQQKEEIKSLLREADLAGCKAYLENWLPAELVELIDESPNE
                     HRVLIFKAINREQAFETYESLDLNLQISLLDILPPRQVQLILNDMSPDSRTALLEDID
                     NDLLNRLLKLLTPKERAIALSLLGYPENSIGRLMTPDYIAIRKEWSVQYALDYIRENG
                     ENSETLNIIYIVDDKGHLIDDIKVAEILLADLHIKIEELIDGKYTALSVTDDEKVAIS
                     EFKKHNRVALPVVDTSQMLLGIITVDDVLQLAEQEYSEDIQKLGAVEALEEPYMEVPM
                     YRLLQKRAPWLIVLFIGEMFTASAMSFFEHEIQRAVVLALFIPLIVSSGGNAGSQAAT
                     LIIRALALGEVGMKDWWRVMRREIISGLMLGLILGSIGFLRVAAWAMFTDIYGPHWLL
                     IGITIGITLIGVCLWGTVMGSMLPIVLKRFGADPATSSAPFIATLVDVTGLVIYFSIA
                     ATILSGTLL"
     gene            complement(2573..3469)
                     /locus_tag="PKX59_08905"
     CDS             complement(2573..3469)
                     /locus_tag="PKX59_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91024.1"
                     /translation="MKNVLFILLAFVITTSCKKKDDDNEAQLIFKFRFDSTQQRQDNF
                     GNAATLPANHGALSPVFNSMSSHYIELAPTAFTQLGSGAVLYRNSETTQGGDNAIVFS
                     ESVLAGNNEEFFRVPFKNIAPGTYQYLRVSLAYQNYDITFGANILGTVYPNLKGTVAS
                     FIGFNSFINSFKIKDSTVTLNANRKQGYWAFETLNQNYPFPGFLPPVISGQAPAGATT
                     VPNPLASTSPIPAGSCVVTGVFSAPFVVTGNETSDKTITVSISTNKSFEWVEHSTPGV
                     YEPANGDTVVDMGVRGLIPIIN"
     gene            complement(3895..4290)
                     /locus_tag="PKX59_08910"
     CDS             complement(3895..4290)
                     /locus_tag="PKX59_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91025.1"
                     /translation="MKELKMCPITKCEPGAKLIGVKIDNDVFDFLKENIMVDEDLFDV
                     MTAVSNPEKRFRFSNVCLETGCKHWTGAACGCVGKVLKNVEDLTLKKDAILPQCSIRE
                     RCRWFYQEGRMACDVCPLLAIKDKDLLEV"
     gene            complement(4517..5266)
                     /locus_tag="PKX59_08915"
     CDS             complement(4517..5266)
                     /locus_tag="PKX59_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOZ91026.1"
                     /translation="MINLVLVDDEVNQINLIKDCVENYFPQIKILGTASSVPEAETLI
                     RSTKPDLVFSDIDMPPYTGFDLLQRFEKIDFGVVFITGFDKYAVRAFEVAAVDYLVKP
                     VTPEDLQRAIKKFEVEAERKRSAEMLQVLLHNHNISQKDEMHIALPKFSGFDVLKLKQ
                     VIHIRGEGNYSRFFLTDNTERLVTKQLGVYEKALTDFDFFRVHKSNMVNLAHVKSVSH
                     NNGGEVIMSNGNTIEIARGRKEDFLLRLKPI"
     gene            5490..8318
                     /locus_tag="PKX59_08920"
     CDS             5490..8318
                     /locus_tag="PKX59_08920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HOZ91027.1"
                     /translation="MLSLLWIAPWITSATNNTTGHFTLPAGISSEDYQQGKVIFKVKD
                     SYRSLCTATGINIQPLNVALNNIGCTAVSKMFPGKEKPTTERNAMHQQYADLSLIYVA
                     QINPSASIEKAINAMLATGVLIYAEPSYIYKTNFTPNDGQIGSQWFLTKIQAFSAWDT
                     QQGDTSVVIGIVDSGTDWDHPDLQNNIKYNYADPINGVDDDNDGYIDNYRGWDMSMND
                     NNPMVDMSDHGSHVSGCAAATTNNGTGVASPGFNCKFLPVKCANATSVTTIDRGYEGI
                     VYAADHGCQIINCSWGGSAGGSVGQDAITYATVNKGALVVCSAGNTYTDIAQYPAAYN
                     YAFSVAATNSSDEKSSFSTYNYSVDISAPGSNIFSTLYNNSYASLDGTSMSAPITSGC
                     AAIVKSQFPTYNNMQVAEQLRMTADNIYGVSGNSGFVDKLGTGRVNLYNAVTQSPKGV
                     RMDNISLSDNNDLAFVAGDTLRITGDVLNLLAPLSNLTVTLSTVSSSVTILNNTINPG
                     AMATLDVVNTATTPFLVKIKPTAPVNSNVVFKLSFTDGNLIVNQYFTVVVNVDYINVN
                     INEVATTITSKGRLFYYGINQTSGLGFDFNGDNLVYDGGLMIGNNNLVSDNCRDGSNF
                     DDDFQKQIAIKAIIPSVKSEFDLTTNFNDNNVTAANRLKVLVRHNTYAWSSPGNSRFI
                     IVEYHIKNTGTNALSTLYAGICADWDIQTYANNKSDVDAVNKMGYSWCTDNGGLYAGV
                     KLLSATPFNSYAIDNVTGGAGGVDATSDFTNSEKYTTLSTSRAQAGNTLPAGNDVIQV
                     VSSGPFNLAGGDSITVAFALIAGDSLADIQQSAANAQIKYDGILTSVQENIAHGSETQ
                     LLNPYPNPNSGNELNIPFYIAKNQINSVNISLTDMTGRTVVDLQNIQTISGMNIQTVN
                     MAGLSNGIYLIKLESAGKIYTGKISVQH"
BASE COUNT         2634 a         1603 c         1596 g         2581 t
ORIGIN      
        1 tttttgcaca catggaagag attgatcttt gctggcgtat gaagcatagt ggtcataaac
       61 tgtattgtat tcctgcatca agagtatatc atgttggtgg tggaacatta cataaaagta
      121 acccgaaaaa gacctatctc aatttcagaa ataatctgat gatgctgtta aaaaatttac
      181 cttcaggcaa tagattttct gtatttacca aacgaatgta tcttgataaa ctggctttca
      241 ttaaattttt tatttcagga aattttaaag atgcttgggc tgtttatagg gcttattttt
      301 atgtgatact tcattttaaa actttaaaga aaaaacgtgt agaaattaaa aatatcaaca
      361 ccaatgtttc ctgtatattt gaaaaaagca ttgttgcaga acattttttc agaggaaaaa
      421 agaagtttac agaattaaat caagattgtt tttcataata attcttaatt gttaatcaat
      481 gaaaataatt tgtgttggtc gaaactatgc tgcacatgca gcagagctaa aaaatccgtt
      541 gcattcagag cctgtcattt ttatgaaacc agaaacggca caattgctgc cacgaatgcc
      601 attttttatt cctgacttca gcaccgacat tcatcatgaa gtggaattgg ttgtaaaaat
      661 caaccgtaca ggaaagcaca ttcaggagca gtttgcaaac acctattatg atgaaatagg
      721 cataggaata gattttactg ctcgcgacaa acaatcgcaa ttaaaagaaa aaggtctgcc
      781 ctgggagtta gccaaagcat ttgatggcag cgcacctaca ggtaagttcc tcaataaatc
      841 attattcaaa tcggttgagg atattaattt ccgacttgaa aaaaatggaa atattgtgca
      901 gcaaggcaac agtagcaata tgctttttaa tattgataaa ataattgcat ttgtttcatc
      961 ctatatcacg cttaaaaaag gtgatttgat ttttacgggt actcccgaag gtgttggcaa
     1021 ggtacaggca aacgatgtat tggatgcatt tattgaagat caacatcttt tgactgttaa
     1081 aataaaatag ttaaaaaaat aattattcct catcagaaca ttaggtcaat acgctaattt
     1141 tacccttatt aatatttaaa tttacgcccg caaaaatcac ttttcaaaca tgcttcagca
     1201 acagcaaaag gaagaaataa aaagcctgct ccgggaggcc gaccttgcag gttgcaaagc
     1261 atatctggag aattggttgc ccgcagagtt ggtcgaactt attgatgagt cgccaaacga
     1321 acatcgcgtt ttaattttta aggctataaa tcgtgaacag gcttttgaaa cctatgaatc
     1381 attagacctg aatctgcaga tttctttact cgacatttta cctccgcgtc aggtacagct
     1441 tattctcaat gacatgagtc ccgattcgcg tacagcattg ctcgaagata ttgataacga
     1501 cctgctgaac cgcttgctga aactgctcac accaaaggag cgtgccattg cactttctct
     1561 gcttggctat cctgaaaatt ctatcggtcg tttgatgaca cccgattaca ttgccataag
     1621 aaaagaatgg agcgtacaat atgcgctcga ctacattcgt gaaaacggtg aaaactctga
     1681 aacgctgaac atcatttata ttgttgatga caaaggacat ctgattgacg atattaaagt
     1741 agccgaaata ttattagccg acctgcatat taaaattgaa gagctgattg acggaaaata
     1801 tactgcactt tctgttactg atgatgagaa agttgccatc agcgaattta aaaaacacaa
     1861 ccgtgtggcc ttgcctgtgg ttgacacttc gcaaatgctg ttagggatta ttaccgttga
     1921 tgatgtttta caacttgcag agcaggaata ttctgaagac atccagaaac ttggtgccgt
     1981 tgaagcgttg gaagagccat atatggaagt gcccatgtat cgtctgttgc aaaaacgtgc
     2041 tccctggctg atagtacttt ttatcggtga aatgtttacg gcttcggcaa tgagtttttt
     2101 tgagcatgaa atccaaagag ctgtagtgct tgcattattt attccgctca ttgtcagcag
     2161 cggtggcaat gcaggctcac aagcagctac acttattatc cgtgcattgg cgcttggcga
     2221 agttggcatg aaagactggt ggcgggtaat gcgcagagaa ataatttccg gattgatgct
     2281 tggactcatt ctgggaagta ttggattttt acgtgttgcc gcatgggcaa tgtttacaga
     2341 tatttacgga ccacattggc tgctcattgg cattaccatt ggcataacgt taattggtgt
     2401 ctgtttatgg ggaactgtta tgggctctat gttgcccatt gttttaaaac gctttggtgc
     2461 cgaccctgca acctcttctg caccattcat tgcaactttg gttgacgtta ccggattggt
     2521 aatctatttt tctattgctg caacaatact ctcgggaacg ctgctttaaa tattaattta
     2581 ttattggtat aagccctctt acacccatat caacaacagt gtcgccatta gcaggttcat
     2641 acactccggg ggtgctgtgt tctacccatt caaagctctt gttagtagag atagatactg
     2701 taatcgtttt atctgatgtt tcatttccgg taactacaaa tggtgcagaa aagacacctg
     2761 taaccacaca tgaccctgca gggattggcg aagttgatgc cagcggatta ggaacagttg
     2821 ttgcaccggc aggagcctgt ccgctgatga caggaggaag aaaacccggg aaaggataat
     2881 tttgatttaa ggtttcaaat gcccagtaac cttgcttacg attagcattt aatgtgacgg
     2941 tgctgtcctt tattttaaat gagttaataa aagagttgaa tccaataaaa gaagcaacag
     3001 tacctttaag gttgggataa acagttccaa gaatattggc accgaaggtg atgtcataat
     3061 tctgatacgc taatgaaaca cgtaagtact gatatgtgcc gggagcaatg tttttaaacg
     3121 gcactctgaa aaactcttca ttgttacctg caagtaccga ctctgaaaaa acaatagcat
     3181 tgtcgccacc ttgtgtggtt tcactatttc tgtatagcac tgccccacta cctaactgcg
     3241 taaatgccgt tggtgcaagt tcaatatagt gcgaactcat ggaattaaat actggcgata
     3301 atgcaccgtg attagcaggt agcgttgctg cattgccaaa attatcctga cgctgttgtg
     3361 tggagtcaaa acgaaactta aatatcaact gcgcttcgtt atcatcatct ttcttcttgc
     3421 atgatgtagt tatgacgaat gccaacagaa taaataatac atttttcatt tgtttgattt
     3481 ttaaattcta tgctgcaata ataatcatat cctataatag gaaacgttgt agttgatgaa
     3541 taattgtact ttagaagtga taaggtgatt atgcaatgaa gtaataattt aagtcctcta
     3601 tacccgttta gttgtatttt ggaattccaa gaaaaataaa acaaataaga atggctttta
     3661 cacttgattg aagatgataa atttgagtta aatatactgc atttttttta aaaagcaaat
     3721 attttttatt catttacatc ctaactccaa attcaaactg caaaatgtat tgctgtttaa
     3781 gtttctgtca aaagaaactt tgcttccaca atgaaactta cattcaagaa agtattttgt
     3841 caattatcaa ttacacacct atggaaatat ttaattacaa attgaaaata atctttacac
     3901 ctccaacaaa tctttgtctt taattgcaag cagtgggcaa acatcgcagg ccatacgacc
     3961 ttcctgataa aaccaacggc atctttcgcg aatactgcat tgcggtaaaa tggcatcttt
     4021 ttttaaggtc aagtcttcaa catttttaag aaccttaccc acacagccac aagctgctcc
     4081 tgtccagtgt ttgcatccag tttccagaca aacattgctg aaacgaaatc gcttttcagg
     4141 attgctgact gcagtcataa catcaaaaag gtcttcgtcc accatgatgt tttcttttaa
     4201 aaaatcaaat acatcattat caatttttac acctatgagt tttgctccgg gttcgcattt
     4261 ggtgatgggg cacattttca actctttcat gtctgtaatt tttgcggttt tataatattt
     4321 actttttttc acttttaaaa ttttctgacc ccggcattcg ttaacaccgg gaatcagatt
     4381 ttttcaattg ggttagcctc agggcgattt ccaaatccga gttgaggata tgacatcaaa
     4441 gtaaaataca attaatttta caacctaaag gttttgtatc aaatgcagat gcggttgtac
     4501 gagggaaata aagctgctaa ataggcttca atctcaataa aaaatcttct tttctccctc
     4561 tggctatctc tatggtattg ccatttgaca tgatgacttc accaccatta ttgtggctta
     4621 cagattttac gtgcgctaag tttaccatat ttgacttatg tacccgaaaa aagtcgaaat
     4681 cggtgagtgc tttttcataa acacctaatt gtttagtgac caaacgctct gtattgtctg
     4741 tcagaaaaaa acgggaataa ttgccttcgc ctctgatgtg aataacctgc tttaatttga
     4801 gcacatcaaa gcctgaaaat tttggaagag caatgtgcat ttcgtccttt tggctgatgt
     4861 tatggttgtg caataaaacc tgaagcattt cagccgacct tttacgttct gcctctacct
     4921 caaacttttt tatggcacgc tgaaggtctt caggtgtaac aggctttacc aaataatcaa
     4981 cagccgctac ttcaaatgca cggactgcat atttatcaaa acctgttata aaaacaacac
     5041 caaagtcaat cttttcaaac ctttgcaaaa ggtcaaatcc ggtatagggc ggcatgtcaa
     5101 tatcactgaa aaccaaatcg ggctttgtgc tgcgtatgag tgtttctgcc tccggtacag
     5161 agctggcagt gcccagaatc tttatctgcg gaaagtaatt ttctacacag tcttttataa
     5221 gattaatctg gttgacttca tcatcaacta gcacaaggtt tatcatggct taaaagtagc
     5281 ggaaatccga caaaaaaact caggttgcaa aaaatatttt gcaacctgag tacctaacct
     5341 aagcctatga aacaatacaa ccaataaaca gcacagcacc aattttgttt tagccgctca
     5401 cttattaact gaatgtgaat gacataatca ttttacatac attcgcatct ctaaaaaatc
     5461 aaacaaaata tatgcttaaa cgctacatca tgctttccct tttatggatt gcgccatgga
     5521 ttacatcagc cacgaacaac acaacaggtc attttacact tcctgcaggt atttcatctg
     5581 aagactacca acaaggaaag gttatcttta aagttaagga ctcttaccgg tcactttgta
     5641 ctgctacagg cataaacata caaccactga atgtggcttt aaataacata ggctgtacgg
     5701 ctgtttctaa aatgtttccc ggcaaagaga agccaactac tgaaagaaat gctatgcatc
     5761 agcagtatgc cgatttaagt ttaatttatg tggcgcagat taatccttct gcttccatag
     5821 agaaagccat caatgcaatg cttgcaacag gtgttttaat ttatgctgaa ccatcttaca
     5881 tctataaaac aaactttaca cccaatgacg ggcagatagg ctcgcaatgg tttcttacaa
     5941 aaattcaggc ttttagtgca tgggatactc aacaaggcga cacctctgtg gttataggca
     6001 ttgttgactc aggcacagac tgggatcacc cggatcttca gaacaatatt aaatacaact
     6061 atgcagaccc aattaatggt gtggatgatg acaatgacgg atacattgac aactaccgtg
     6121 gctgggacat gagcatgaac gacaacaatc caatggtgga tatgagtgat catggctcgc
     6181 atgtatcagg atgtgctgct gctacaacta ataatggaac aggtgttgcc tcgcccggat
     6241 ttaattgtaa gttcttaccg gtgaagtgcg ccaatgccac ctccgttact acaattgaca
     6301 gaggttatga aggcatagtt tatgctgccg atcatggatg ccagattatc aattgctcat
     6361 ggggtggcag tgcaggtggc agtgttggac aagatgctat tacctatgca acagtaaata
     6421 aaggtgcctt ggttgtttgt tctgcaggaa acacctacac cgatattgca caatatcctg
     6481 ctgcatataa ttatgcattt agtgttgctg caacaaactc cagcgatgaa aaatcatctt
     6541 tttctaccta taattattct gtagatattt ctgcacctgg ctcaaatatt ttctctaccc
     6601 tatataacaa cagttatgct tcgttagatg gaacttcaat gtcggcacca attacctcag
     6661 gttgcgcagc catagtaaaa tcacaattcc cgacctataa caacatgcag gtggcagagc
     6721 aattacgaat gactgccgat aatatttatg gtgtttcggg taacagtggt tttgttgata
     6781 aattaggtac cggacgtgtg aatctttata atgcagttac acaaagccct aaaggagtgc
     6841 gtatggataa tatcagcctg tctgataaca atgaccttgc atttgttgcc ggtgatacct
     6901 tacgaattac aggtgatgtg ctaaaccttc ttgcacctct ttcaaatctt acagttacac
     6961 tttcaacagt atcaagtagt gtaactattt tgaataacac catcaatccc ggtgctatgg
     7021 caactcttga tgtggtgaac actgccacca caccattttt agttaaaata aaacctacag
     7081 ctccggtaaa ttcaaatgtt gtgtttaaac tttcttttac tgatggcaac cttatcgtta
     7141 accaatattt tactgttgta gtaaatgtag attacatcaa tgttaacatc aatgaagtgg
     7201 caacaaccat aaccagcaaa ggacgtttgt tttattatgg catcaatcag acatcaggat
     7261 taggttttga ttttaatggt gataatttgg tttatgatgg tggattgatg attggcaaca
     7321 acaatcttgt ttctgacaac tgtcgtgacg gttctaattt tgatgatgat tttcaaaaac
     7381 aaattgctat taaagctata attccctctg taaaatcaga atttgacctg acaacaaatt
     7441 ttaacgacaa taatgtcact gctgcaaaca gactgaaagt tttagtcaga cataacacat
     7501 acgcatggag ttcaccgggc aactcgcgtt ttatcattgt tgaatatcat attaaaaata
     7561 caggcaccaa tgcattgagt accctctatg ccggtatttg tgccgattgg gatattcaaa
     7621 cctatgccaa caataagtct gatgttgatg ctgtaaataa aatggggtat tcgtggtgta
     7681 cagataatgg cggactttat gcaggagtaa aattgttgtc agcaacacca tttaattctt
     7741 atgctattga caatgtaacc ggtggtgccg gtggtgttga tgcaacaagt gattttacca
     7801 attcagaaaa atatactaca ctaagcacaa gtcgtgcgca ggcaggaaac acacttcctg
     7861 caggtaatga cgtgattcag gtggtaagtt caggaccatt taatcttgcc ggtggtgatt
     7921 caataacagt tgcctttgca ttgatagcag gtgacagcct tgccgatatt cagcaaagtg
     7981 ctgctaatgc acaaataaaa tatgatggta ttttaacttc tgttcaggaa aatattgctc
     8041 atggatcaga aacacaatta ctgaatccct accctaatcc taacagtgga aatgaattaa
     8101 acataccttt ttatattgca aaaaatcaaa taaattctgt aaatatcagt ctgacagata
     8161 tgacaggaag aacagttgtt gatcttcaaa acatccagac aatttcagga atgaatatac
     8221 agacagttaa catggcaggg ttaagcaatg gtatttatct gattaaatta gaaagtgccg
     8281 gcaaaatata caccggaaaa atttctgtac agcattaatt tttaaattaa ccataattaa
     8341 tatttcaaac cacacagaca atggtcgttc tgtgtggttt gatttttcta ccttagccaa
     8401 gagattaata caaa
//
LOCUS       DAOOOI010000080         8202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1725_length_8202_cov_28.806186, whole genome shotgun sequence.
ACCESSION   DAOOOI010000080 DAOOOI010000000
VERSION     DAOOOI010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8202
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1725_length_8202_cov_28.806186"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PKX59_08925"
     CDS             complement(<1..254)
                     /locus_tag="PKX59_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91028.1"
                     /translation="MDLSEAFEHLLENVDKNKYTKGTVASWKNRFNANKLSYEKMHEL
                     LLDNNYKCFKSDEVWLQPGSDKPYEFAVLDKDEILIYKEI"
     gene            complement(265..462)
                     /locus_tag="PKX59_08930"
     CDS             complement(265..462)
                     /locus_tag="PKX59_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91029.1"
                     /translation="MAKEIKEPVKYTCTSCGGDAYIAFVPETIKRGKLKGQQRAGWNG
                     IVKPGERICLPCGKKRQLKIL"
     gene            complement(467..769)
                     /locus_tag="PKX59_08935"
     CDS             complement(467..769)
                     /locus_tag="PKX59_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91030.1"
                     /translation="MVQSLKNSVYVCKDYVPYQITKNGTFNVDKNEYDLELGWLQKHE
                     KTDIIGETIDTYVVRIIHGNHGIWIGDIVVSLYYFLPLGVHKSRFVSWNNNQLKLI"
     gene            complement(786..1046)
                     /locus_tag="PKX59_08940"
     CDS             complement(786..1046)
                     /locus_tag="PKX59_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91031.1"
                     /translation="MRVTEKIDRPMTENDFDSFNFKSDSDCSEQEFMNKYDFMVQDKR
                     LTVEYLKIKYKWMKGEATWRDVCVEYFKTNAYLTSVKNIKDV"
     gene            complement(1051..1518)
                     /locus_tag="PKX59_08945"
     CDS             complement(1051..1518)
                     /locus_tag="PKX59_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91032.1"
                     /translation="MSIVIDFKKEKLSEITKETFKEAIFLAYKDGVTSVAPLGFSGNT
                     IILHGSSNEKTSRVSQVSVRNYSGDAELLITDIKGSFLFYGRFRVDLGIDFVAHQYWI
                     IFKKVRKYILKEVPRISEFKDIEIPHNCNRSSGFDILHHAQLNYYKKHDSLLN"
     gene            complement(1515..2006)
                     /locus_tag="PKX59_08950"
     CDS             complement(1515..2006)
                     /locus_tag="PKX59_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91033.1"
                     /translation="MSIYNPDNKSDTVERIVKDFLFSRRIATDVIALLIESGKSEKEV
                     REYFVNAFKDNHVSTVQAESWYSMLLAIANNDYLKKSMKIMDGEYIRRPSAAYFIMQY
                     YIDFEEFTKQQSVLIIADHDKIAEEKAKAFSSTIKSISNVEVSRISKFEFDLLKNDLK
                     IIR"
     gene            complement(1981..2445)
                     /locus_tag="PKX59_08955"
     CDS             complement(1981..2445)
                     /locus_tag="PKX59_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91034.1"
                     /translation="MSTLVTERNIFEKEWCVDNFKDYAVIDFTTRERIVDVVYSKPSM
                     QKKIALVISAVPEMIDALTMISRVLDEQLTADAKENPDIAEALKVYEKIIEKVVPIEF
                     TLNCGKVIEKGDAYYIVENGSVVWQQLSHKSDLEKIGFETPTQANEYLQSRQ"
     gene            complement(2473..2799)
                     /locus_tag="PKX59_08960"
     CDS             complement(2473..2799)
                     /locus_tag="PKX59_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91035.1"
                     /translation="MFYDAYSNYRKFMHEDSVYSAFMAKESEVEIELPMVQDYKLREI
                     LDAWNSYGGMRWRIRYVNDYWYVYQWGVGSWIWEKLVSHIERDKFHNELYIATVDARL
                     IKTTLF"
     gene            complement(2818..4437)
                     /locus_tag="PKX59_08965"
     CDS             complement(2818..4437)
                     /locus_tag="PKX59_08965"
                     /inference="COORDINATES: protein motif:HMM:NF025085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HOZ91036.1"
                     /translation="MFDKEFYPTPKHVITKMLQPYMDQKKRVYGGTANYFTDISILEP
                     SAGKGDILDYINDKFSGKRAGLRYYCIERNQDLTHILNEKDYRVIAHDFLQYSGDYYF
                     DFIIMNPPFSNGDEHLLKAWDVLRGGDIVCLLNTETLTNPNTERKQHLLKLIEKHGSF
                     EHLGAVFADSERPTNVRVSMVRLHKVEEESAFNFKFEKVTKESQFDLNEDILGNEIAR
                     KDLVGNMMLQYDELRKAYIDYNKARSRVSFYSNGLMTQYCSIFKDVIDKLNQNTLRGD
                     YNQFCDLVKKEIWRVIFGKIDMDKYMTHDVRQNFSKYIDKQGAMDFTKENVAQVVEML
                     FTNRFTILDKAVIDVFDIFTRHHKENRYLIEGWKTNDKWKVNRKVILPYYVKYGEYMS
                     QHDLKQFGDKFSISYHRASEYTDIDKVMCYITGKDFKNCTTISEALKDSFQRLGHVKN
                     EKFDNTCTSTFFNIRFFKKGTIHIEFRDAKLWEQFNLMACAGKKWLPETEQREYDAKM
                     QYREEPELTITPKMIEANLEPITAQFELNFV"
     gene            complement(4526..5755)
                     /locus_tag="PKX59_08970"
     CDS             complement(4526..5755)
                     /locus_tag="PKX59_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91037.1"
                     /translation="MKTVKDIIKKIIKDGEVIGVNGRVLTYISKENDGTYAVVMHNDL
                     VPKLRSYATEQQLIKWFCNTSETLPVRTIEQMITEVNDAGIYYINEQGVYIKYISENR
                     WNDGKHRAVVCGPGIRASGNNAPYSDEQLIKWFGTTKWRLAGLLCNELSDNRHTVTEF
                     QDYIAQIDYNDVSPIDDVRYDKIKPLTGKITEVIQSDAIIPAYKPKKVKNVVPKPEKR
                     KNYKGTPKEQLPPEVITYRKGLSAQQVYKRRINILDRLDFDKVKALFDAVIEAQDAYE
                     YDLSEEGIIAKNINKTTKESFCRYGDANHITRTIRVKYLNDKVGVALDVQAYWMQENF
                     NKDIEPDDLAAFITNYENGAEDYPLYAKIDECKQEWKNYTGINYDSKFIKHYINRKLN
                     AEFKEYSKVPAGDGDIF"
     gene            complement(5752..6366)
                     /locus_tag="PKX59_08975"
     CDS             complement(5752..6366)
                     /locus_tag="PKX59_08975"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91038.1"
                     /translation="MHLTTVQGYGFFECSSAFQKSIRRGDETNALYFAVELFNSGYDE
                     YVWKRMKVITSEDIGLAEPNAPAIIQSLYEMYNDAKAKKHEKMPQRLHYVHAIMYLCR
                     CKKSRLIDWTMIALWREHKLEKREVPDFAFDMHNAKGKQKGRGIDHFYNDGTELTNHV
                     PQDGEEEWKQKAWKLHKEHPLPDLPVKKEKGKKNDLFDNQNSEV"
     gene            complement(6455..7555)
                     /gene="glf"
                     /locus_tag="PKX59_08980"
     CDS             complement(6455..7555)
                     /gene="glf"
                     /locus_tag="PKX59_08980"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012956328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HOZ91039.1"
                     /translation="MNYLEKYDYLIVGSGLFGSTFAERMHRKGKRVLIIEKRNHIGGN
                     VWTEEMNGIHVHKYGPHIFHTNSEDVWTYVNRFCKWRQFQYSPIANYNGELYSLPFNL
                     HTFYQFYGIKTPAELQSKIEQITSKYQKPSYDNVEDFALAMLGEDLYRKLIYGYTKKQ
                     WNAEPVTISKSIIARLPIRQMFDSNYFNDLHQAIPVGGYAELVTNMTKKIEVIKNTDF
                     LSAKKEFENIAEKVIYTGSIDEYYGYELGELEYRSLRFETEQLPTENYQGVAVMNYTD
                     EHTPFTRVIEHKHFERMQSKSTVITKEFPAEWKKGMERFYPVNTEMNNALLQKYKKIT
                     NKNTLFGGRLGSYQYYNMDQIIASALKLSNSL"
     gene            complement(7533..8174)
                     /locus_tag="PKX59_08985"
     CDS             complement(7533..8174)
                     /locus_tag="PKX59_08985"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HOZ91040.1"
                     /translation="MITHDYKLIYIHIPKCAGRSITDMFNQRFDHCTAQYYQNEYPRQ
                     FKQYDVFTIVRNPYDRYVSMWNYIKNHRRHQKEDIYNQYFDDDRYDFKHWLLKNIFAY
                     KGGYPATSAEGGRGFDDKIGSAFWFSSQYSRLSNWNKSLIVPNVFHFEKMDEVYNFLS
                     ERMEQVAAIPHLNQSKRKEWTYYYDDEIIEHLHKFQPFLTDIEKLGYELLREV"
BASE COUNT         2243 a         1723 c         1276 g         2960 t
ORIGIN      
        1 aatatttctt tgtaaatcaa aatctcatcc ttatccaata ccgcaaactc atacggcttg
       61 tcggagccag gttgcaacca aacctcatcg ctcttaaaac acttgtaatt gttatcgagc
      121 aacaactcat gcattttttc ataggatagc ttgttcgcat taaatcggtt cttccaactt
      181 gctaccgttc ctttggtata tttgttctta tctacatttt cgagtagatg ttcgaatgct
      241 tcagataaat ccatagttat tattttataa aattttaagt tgacgttttt ttccgcatgg
      301 cagacagata cgttctcccg gcttaactat accgttccaa cctgctctct gttgtccttt
      361 caattttcct cgctttatag tttccggtac aaacgctatg tatgcatcac caccacaact
      421 tgtgcaggta tatttaaccg gctcttttat ttcttttgcc ataatactat attaatttta
      481 actgattatt attccaactt acaaacctgc ttttatgtac tcctaaaggt aaaaagtaat
      541 ataaacttac tactatatca ccaatccata ttccatgatt tccatgaata attctaacga
      601 cataagtatc tattgtttct ccaatgatat ctgttttttc gtgtttctgc aaccaaccta
      661 attctaagtc atactcattt ttatcaacgt taaaagtacc gtttttggta atctgataag
      721 gcacataatc cttgcaaacg taaactgaat tttttaatga ctgcaccaat tcaggtagtt
      781 taatattata catcttttat attttttaca cttgttaaat aagcatttgt tttgaagtat
      841 tccacacaaa cgtcacgcca tgtggcctct cctttcatcc atttgtattt tatcttcaga
      901 tactcaactg tcagccgttt atcctgtacc ataaaatcgt acttgttcat aaactcctgc
      961 tcggagcaat cactatctga tttaaaatta aatgaatcga aatcattttc tgtcataggt
     1021 ctatctattt tttctgttac tctcatatag ttaattaagc agtgaatcgt gttttttata
     1081 gtaatttaat tgtgcatgat gtaaaatatc aaaaccacta cttctattgc aattatgtgg
     1141 tatttcgata tccttaaact cacttattct tggtacttcc tttaaaatat attttcgtac
     1201 tttcttgaat ataatccaat attgatgtgc aacaaaatct atacctaagt caacacgaaa
     1261 tcgaccataa aataaaaaac tacctttaat atcggtaatt aataattcag catcaccaga
     1321 gtaatttcga acacttactt gtgatacacg acttgttttt tcattactac taccatgtag
     1381 tattattgta tttccactaa aacctaatgg tgcaacactt gttacaccgt ctttgtatgc
     1441 taaaaatata gcttctttga aagtttcttt tgttatttca gataactttt cctttttaaa
     1501 atctattact atgctcatcg tataattttt aaatcgtttt ttaataaatc aaattcaaat
     1561 ttcgatatcc gggaaacttc aacgtttgat atgcttttta ttgtggagga aaatgctttt
     1621 gccttttctt cagcaatctt atcgtgatct gctataatca atacactctg ttgcttagtg
     1681 aactcctcaa agtctatgta atattgcatg ataaaataag ctgcagacgg cctacggata
     1741 tactcaccgt ccattatctt catgcttttt ttaaggtaat cgttattagc tattgctaaa
     1801 agcattgaat accacgattc tgcctgaacg gtagatacat ggttatcttt gaatgcatta
     1861 acgaaatact ctcttacttc cttttcagat ttacccgatt caatcagcaa tgcaattaca
     1921 tcggtagcaa tcctacggct gaataagaag tcttttacaa tacgttcaac ggtatcgctt
     1981 ttattgtcgg gattgtagat actcatttgc ttgtgttgga gtttcaaatc ctatcttttc
     2041 taaatctgat ttgtggctta gttgctgcca tactacacta ccattttcaa ctatgtagta
     2101 tgcatcacct ttctcgataa ctttaccaca attcaaagta aattcaatcg gaactacctt
     2161 ttcgataatc ttctcgtaca ctttcaatgc ctcggctata tccggatttt cttttgcatc
     2221 tgcagtcagc tgctcatcca gcactctcga tatcatcgtt agtgcatcaa tcatttccgg
     2281 tactgcagag ataaccaatg ctattttctt ttgcatactt ggtttactgt aaactacatc
     2341 gacaatgcgt tctctagtcg tgaagtcaat caccgcatag tctttaaaat tgtcaacgca
     2401 ccattctttt tcaaagatgt tacgttctgt tactaatgtg ctcataaata ttgtttattg
     2461 gtgaaaaatt atttaaaata aagttgtttt tattaatcgt gcatcaactg ttgcaatgta
     2521 taattcattg tggaatttat ccctctcaat atgtgatacc aacttctccc atatccagct
     2581 gccaactccc cattgataaa cataccaata atcatttaca tacctgatac gccaacgcat
     2641 cccaccataa ctattccatg catcaagtat ctcacgtaat ttataatcct gtaccatagg
     2701 taattcaatt tcaacctcac tttcttttgc cataaatgct gaataaacag agtcctcgtg
     2761 catgaatttt ctataatttg aataagcgtc gtaaaacatt gtttttgatt ttataagtta
     2821 tacaaaattc aactcgaatt gtgccgttat tggctccaaa tttgcctcaa tcattttcgg
     2881 tgttatagtt agttcaggtt cttcacgata ctgcatttta gcatcgtatt cacgttgctc
     2941 tgtttccggt aaccacttct tcccggcaca cgccataaga ttgaactgct cccacaactt
     3001 agcatcccga aattctatgt gtattgtacc ttttttgaaa aatcggatat taaagaacgt
     3061 gcttgtgcag gtattgtcaa acttctcatt tttcacatgg cctaaccttt ggaaactatc
     3121 ttttaatgcc tccgaaatag tagtgcagtt tttaaaatct ttccctgtaa tgtagcacat
     3181 cactttatca atgtcggtgt actcgctcgc tctgtgatat gagatactga atttatctcc
     3241 aaactgtttc aggtcatgtt ggctcatgta ctcaccgtac ttaacgtaat atggcaggat
     3301 aaccttacgg tttactttcc atttgtcatt cgttttccaa ccctcgatta gataccggtt
     3361 ttctttgtgg tgccttgtaa agatgtcgaa tacatcaatt acggccttat ctaaaatcgt
     3421 aaatctgttg gtgaatagca tttctacaac ctgggccacg ttctctttgg taaaatccat
     3481 tgctccctgt ttgtcaatgt acttggagaa gttttgacgt acatcatgcg tcatgtattt
     3541 atccatatca atcttaccaa atatcacacg ccatatctct tttttgacga gatcgcaaaa
     3601 ctgattatag tcaccacgta atgtattctg attaagttta tcaattacgt ctttgaaaat
     3661 actgcaatac tgtgtcataa ggccattgga gtagaatgat acccggctcc ttgctttatt
     3721 gtagtcaatg tatgccttac gcaattcgtc gtactgaagc atcatgttac ctaccaaatc
     3781 ctttcgggca atttcgttac ccaaaatatc ttcattcagg tcgaactgtg attcctttgt
     3841 aactttctca aatttgaagt tgaatgcact ttcctcctca actttgtgga ggcgtaccat
     3901 gctcactctt acattggttg gccgttcact gtcagcaaat accgctccca aatgctcaaa
     3961 actaccatgc ttttcaatca gcttcagcag atgttgtttc cgctctgtgt tcggattggt
     4021 taacgtttcg gtattcagta agcatacgat atctcctcca cgtaatacgt cccacgcttt
     4081 cagcaggtgc tcgtcgccat tcgagaacgg agggttcata atgataaaat cgaaatagta
     4141 atctccggaa tactgcagga aatcatgcgc tattactcgg tagtctttct cattcaggat
     4201 atgcgtcaaa tcttggttgc gttcaatgca gtagtaccgc aatccggctc tcttacctga
     4261 aaacttgtcg ttaatgtagt ctaagatgtc gcccttaccg gcagacggtt cgagtattga
     4321 tatgtcggta aagtaatttg cagttccacc ataaactcgc tttttttggt ccatataagg
     4381 ctgtaacatc ttagtaatta catgctttgg agtagggtaa aattctttgt caaacatgac
     4441 ttttaattta ttggttaaaa aatttgttgt tgctacgata aaaatacaaa agagtttgca
     4501 aatatgcaaa ctctctcact tttttttaga atatatcacc atcacctgca ggaactttag
     4561 aatactcttt aaattcagcg ttcaattttc ggttaatgta atgtttaatg aacttactgt
     4621 catagttaat tcctgtgtaa tttttccatt cttgtttaca ctcatcaatc tttgcataaa
     4681 gcggataatc ttctgcacca ttctcataat tagtaatgaa tgcagctaaa tcatccggct
     4741 caatatcttt attgaaattc tcctgcatcc aatatgcctg aacatctaat gcaactccta
     4801 ccttatcatt tagatatttt actctgatag ttcgtgtaat gtgatttgcg tcaccatatc
     4861 tgcagaacga ttcctttgta gtcttattga tatttttggc aataataccc tcctctgata
     4921 aatcgtattc gtatgcatcc tgtgcctcaa tgactgcatc aaataatgct ttcactttgt
     4981 cgaaatccaa tctatcgagg atattaatac gacgtttata aacctgttga gcgctcaatc
     5041 ctttccggta tgtgataact tcaggcggta actgttcctt tggagtgcct ttgtagttct
     5101 tacgtttttc aggctttggc accacatttt taaccttttt aggcttgtag gcaggtatta
     5161 tagcatccga ttgaataacc tcggttattt tgcctgtcaa tggcttgatt ttatcgtatc
     5221 ttacatcgtc aataggactt acatcattat aatctatttg tgcaatgtaa tcctgaaatt
     5281 ctgtaactgt gtgacggtta tctgataact cattgcagag taaacctgct aacctccact
     5341 tagttgtacc gaaccattta atcagctgct catcactata cggagcattg tttcctgatg
     5401 ctcttatacc agggccacaa actaccgctc tgtgctttcc gtcattccac ctgttctcac
     5461 tgatatattt gatgtaaact ccttgctcat taatgtaata gatacctgcg tcattcactt
     5521 cagttatcat ctgttcgata gttcttacag gtaatgtttc tgatgtattg cagaaccatt
     5581 taatcaactg ttgttctgtt gcatacgatc gcaattttgg caccaaatca ttatgcatca
     5641 caactgcata cgttccgtca ttttccttac taatgtaagt cagtacccgg ccattaactc
     5701 ctataacttc accgtcttta atgatttttt taatgatgtc ttttaccgtt ttcatacctc
     5761 cgaattttgg ttatcaaata aatcattctt tttgcctttc tcttttttta caggcaggtc
     5821 gggtaatgga tgctccttat ggagtttcca tgctttctgt ttccattctt cttcaccgtc
     5881 ctgcggaacg tgatttgtca actccgtacc gtcattgtag aaatggtcga tacctctgcc
     5941 tttctgtttg ccttttgcgt tatgcatatc aaatgcaaaa tccggaactt cacgtttctc
     6001 taatttatgc tctctccata atgcaatcat ggtccaatca attagacgtg attttttgca
     6061 tctgcacaaa tacataattg cgtgtacata gtgcaaacgt tgaggcattt tttcgtgctt
     6121 tttggctttc gcatcattat acatttcgta taacgattga atgatagcag gagcattcgg
     6181 ctcggctaat ccaatgtctt cagaggtgat tactttcata cgtttccaaa cgtactcatc
     6241 gtaccctgaa ttaaagagtt ctactgcaaa gtagagtgcg tttgtttcgt cgccacgacg
     6301 tattgatttt tgaaatgcag aactgcattc aaagaaccca tacccttgta ctgttgttaa
     6361 gtgcatagtt tcaaatttta attgttgcta ccacaaattt aaacaaaagt ttggctatat
     6421 gcaaacgtta ttttattaaa atataaatac ttgattataa actatttgat agctttaatg
     6481 cagatgcaat aatttggtcc atattgtaat actgataact acctaaacga cctccaaata
     6541 atgtattttt atttgttatt tttttatatt tctgaagtaa cgcattattc atttcagtat
     6601 tgacaggata aaaacgttcc ataccttttt tccattcagc aggaaactcc ttagtgataa
     6661 cggtgctttt agattgcatc cgttcaaaat gtttatgctc tatcactctc gtaaatggag
     6721 tgtgctcatc tgtgtaattc ataacggcca cgccctggta gttctccgtt ggcagctgct
     6781 ccgtttcaaa tctcaaacta cggtattcaa gttcacccaa ttcgtaaccg taatactcat
     6841 cgatactgcc tgtgtaaatc actttctctg caatattttc aaattctttt tttgctgaaa
     6901 gaaaatcggt gttttttata acttcaattt tctttgtcat gttcgttacc aattcagcat
     6961 aaccacctac cggtattgct tggtgcaaat cgttaaaata attactgtcg aacatctgcc
     7021 gtattggtag ccgggcaatg atgcttttgg atattgttac cggctctgca ttccattgct
     7081 tttttgtgta tccataaatc agctttcggt ataagtcctc tcccaacata gctaatgcga
     7141 aatcctccac gttatcataa ctcggtttct gatacttcga cgtgatctgc tcaatcttac
     7201 tctgaagttc tgcaggagtt ttgataccat agaattgata gaacgtgtgg aggttaaatg
     7261 gtagtgaata cagttctccg ttgtaattgg caatcggtga atactgaaac tgcctccact
     7321 tgcagaaccg gttaacgtat gtccatacgt cctcggagtt ggtgtggaat atgtgagggc
     7381 cgtacttatg gacgtgaata ccattcattt cttccgtcca tacgttgcct ccgatatggt
     7441 tacgtttctc aataatgaga actcgtttgc ctttcctgtg catccgctct gcaaacgtac
     7501 ttccgaataa accggaacct acaatgaggt aatcatactt ctctaagtag ttcatatcct
     7561 aacttttcta tatccgttaa aaatggctga aacttatgta ggtgttcgat tatttcatca
     7621 tcgtaataat acgtccattc tttacgtttc gattggttaa gatgtggtat tgctgctact
     7681 tgttccatac gctctgataa gaaattataa acctcatcca ttttctcaaa gtggaataca
     7741 tttggcacaa ttaggctctt attccaatta ctcaacctgg aatactgtga actgaaccaa
     7801 aatgcactgc ctatcttatc gtcaaatcca cgtccaccct ctgcacttgt tgcaggataa
     7861 ccacctttgt acgcaaagat atttttcagt agccaatgct taaaatcata ccggtcgtcg
     7921 tcgaaatact gattgtagat gtcctccttt tggtgccgtc tgtgattttt aatgtagttc
     7981 cacattgaaa cgtacctgtc atacggattc cgtactatcg taaacacgtc atattgcttg
     8041 aattgacgag gatattcatt ctgatagtat tgtgcagtac aatggtcgaa acgttgatta
     8101 aacatatcgg taatactgcg tcctgcacac ttaggaatat gaatataaat cagtttgtag
     8161 tcgtgtgtta tcatgtctta atacggcaat ttaattacta at
//
LOCUS       DAOOOI010000081         8154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1735_length_8154_cov_12.301148, whole genome shotgun sequence.
ACCESSION   DAOOOI010000081 DAOOOI010000000
VERSION     DAOOOI010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8154
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1735_length_8154_cov_12.301148"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1449
                     /locus_tag="PKX59_08990"
     CDS             163..1449
                     /locus_tag="PKX59_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOZ91041.1"
                     /translation="MMHATIEKGAYTASDMAETLIGSEIIKLAGEVNEQIKKGNKIFN
                     LTIGDYDPAVFPIPDALKNEIIKAYQNGYTNYPVANGQPELRNAVAGYINKLQKLQYS
                     SNEILISGGARPLIYALYKTVVNPGERVVFPIPSWNNNHYCHLSQAEAVAVETQPQNN
                     FMPLASELKSHLSSAALVALCSPLNPTGTVFSSSQLMEICELILQENKRREGKKPLYL
                     MYDQIYMALTFGKTKHVDPVSLLPEMRPFTIYIDGLSKAFSATGLRVGWAFGPVGIID
                     KMKSILSHVGAWSPKAEQVATAHFLTQEKEVDVFLSHYKSEVEKRLNAFYDGFIKMKN
                     DGLPVNAIDPQAAIYLTVQFDILNKVMPDGKRINNSNDITSFLINYAKVALVPFSAFG
                     ATTTSNWFRLSIGTARMEDIEQMFVSLRKALALLGD"
     gene            complement(1429..2298)
                     /locus_tag="PKX59_08995"
     CDS             complement(1429..2298)
                     /locus_tag="PKX59_08995"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ91042.1"
                     /translation="MHISILLWGFTGIFGKAIEMNAIMIVWYRMIISAVALIPFMVKH
                     KQFILPDKKRLASIIITGFVVCLHWLTFYASIKASNVSIALSCFASVSLFSALLEPLF
                     YRKKINKQNIILALFVLVGIYIIFAFQQLYAMGIVLALISALLGALFTILNKIFVSND
                     EPAPVTFIELISGFVFLSVLLPLILPAFNVSFQIPSKIDWVWLLLLSVVCTSIAFTLS
                     LEALKKVSAFTMNLSVNLEPLYSIVLAIILFKENELLNNGFYIGAVIVISSVFIHSLM
                     QYRVRDKSVTEQG"
     gene            complement(2323..2895)
                     /locus_tag="PKX59_09000"
     CDS             complement(2323..2895)
                     /locus_tag="PKX59_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOZ91043.1"
                     /translation="MNHLKQKIILASRSPRRQQLLQMMGIEFEVRTKETVEDYPEHLQ
                     NEQIVLYLASKKAIAFENEIQENELLISADTIVVHQNKVLNKPADFNEAIEMLTTLKG
                     NMHEVYTGVCIASIHKKELLYDRSEVYFRNYTTEDMHYYISNFKPYDKAGSYGIQDWF
                     GLTCIEKINGCFYNVMGLPTRKVYQQLINF"
     gene            complement(2964..3770)
                     /locus_tag="PKX59_09005"
     CDS             complement(2964..3770)
                     /locus_tag="PKX59_09005"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HOZ91044.1"
                     /translation="MAKKQSFGKRILLVTLLLCGIAVLAFFYRNHLIRYGYKAWRLEK
                     RLFNKETGSATKISFPKGYTVHGIDVSRWQTDVDFSKLKAADLNGDTINFSFAFIKAT
                     EGILLEDPTFIDNWESAQKNNIIRGAYHYFLPNADVSLQAKNFISSVKLKSGDLPPVI
                     DVEEKGRLTKKELVKKLKLFIAVIEKQYKVKPILYSNISFIEDYLADDFPDYNFWIAH
                     YYEDELKIEKSVKWLFWQHSDRVSIPGSSSSYDANVFNGSKKDLKKLLIK"
     gene            complement(3885..4577)
                     /locus_tag="PKX59_09010"
     CDS             complement(3885..4577)
                     /locus_tag="PKX59_09010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOZ91045.1"
                     /translation="MKKIICIIATIVPLMALSQTDKSQAKLQAEKSQNKETMVEITTN
                     LGTMRFKLYNETPLHRDNFIKLANEGFFDSTLFHRVIKNFMIQGGDPQSKNAAAGAML
                     GNGGPGYTIPAEFNTALYHKKGALAAARLGDQVNPQKASSGSQFYIVQGKPAADNELT
                     MMENAKHMKYTPEQRKEYTSVGGTPFLDMDYTVFGELVSGMDVLDKIAAEATNPQNRP
                     LQDIRMTVKVIK"
     gene            4665..5030
                     /locus_tag="PKX59_09015"
     CDS             4665..5030
                     /locus_tag="PKX59_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91046.1"
                     /translation="MKSSLRKTHLVLTSLIVMTVLFTFSCKKEGPCEAIITITDSAGV
                     AVRGATVILRQDAVVNPTTGVQANVYQEGVTDGYGKASFEFKLEAVLNIEASKGPKLG
                     KDYVRLEQSKTVYRTVIIK"
     gene            complement(5023..5397)
                     /locus_tag="PKX59_09020"
     CDS             complement(5023..5397)
                     /locus_tag="PKX59_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009054276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91047.1"
                     /translation="MNETNIQKIKHKWGELLRQLEITFGEKPDLQGMIFLIGVQELGK
                     GYQNFTKEEKQDLMHIATCKLLSYDGYYKFTGVDSDGWPQYEKAKTMPQMPLKEQDLL
                     LKINIIRYFEEDGYELEKSEVI"
     gene            5495..6628
                     /locus_tag="PKX59_09025"
     CDS             5495..6628
                     /locus_tag="PKX59_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91048.1"
                     /translation="MNLLRLKLAIIAVFVAWLLPLAEVSAKNQPVDIVFTLDLSGSTN
                     GLIDDVRDNIWGMNNELTRLYPNADIRFAVVGYSRPSFGSKNQFVKVISPFTSNIDFI
                     ATELYKLKPNIEKGDQYVGAAIRASLDMLSWSHEKNAVKQIFLTGNGSVFLGAFDVVE
                     SCDLAKEKGITVNSLYCYSSLRAKEISGWYKISEITGGKSIDVKVHKRLPDYATVTDF
                     NRLQMLATELNKTYIYYGKEGRDKFKAMVSNEKNVLNARHSAFEDLLYHKISDRFQGK
                     QSDWDLVDYLKSRNGNLKNVDARLLPDSLKNINPEQLLTKLMILKERRSYLLSQIRQL
                     LPFDRQDKLTAYFNSKQSDSDMIFDRQVMMVVKDEMQSDLAAN"
     gene            complement(6632..7342)
                     /locus_tag="PKX59_09030"
     CDS             complement(6632..7342)
                     /locus_tag="PKX59_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HOZ91049.1"
                     /translation="MKNRRDFIKTSALAAGTLALYGIGSKVAIASETNEDNLFKLPDL
                     EYGFEALEPYIDTLTMEIHYTKHHQGYVNNLNKAVVAEKLQGVSLNDLLGKVSKHSVA
                     IRNNAGGHYNHSFFWRLMRPGGAKSPKGELAEAIIRELSGTDNLQKAFSDAAKNRFGS
                     GWAWLIKSADGKLSVTSTPNQDNTLMDIAEVKGKPILGIDVWEHAYYLHYQNKRADYI
                     NAWWNLINWDVALENYNS"
     gene            complement(7354..7854)
                     /locus_tag="PKX59_09035"
     CDS             complement(7354..7854)
                     /locus_tag="PKX59_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOZ91050.1"
                     /translation="MKAIIKGQEINLNFDSKKKVLTKNNQQVEADIKKISESSLSILL
                     QNKSYKAELLEANYNEKQFKIRVNGNVYQVALKDKYDELLQQLGMDKMFAQKVNDLKA
                     PMPGLVLDILVSEGQAIQKGDSLLILEAMKMENNLKATNDAVVKKIKVLKGDKVEKNT
                     VLIELS"
     gene            complement(7893..>8154)
                     /locus_tag="PKX59_09040"
     CDS             complement(7893..>8154)
                     /locus_tag="PKX59_09040"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HOZ91051.1"
                     /translation="GATRYGVIGSVIGMLLGIFLTPVGMIMGTIIGAIVGDLLAGKNT
                     RDALRSGLATVGGTFISTGIKLVGAGIMTWHFIRSTISYYAD"
BASE COUNT         2541 a         1490 c         1433 g         2690 t
ORIGIN      
        1 ctctaaggta ttattattaa ttgaattttt tttttcagaa gtgtgcaaag ataaaaaaac
       61 aaacccattt ttagggtgat attttattat tccggtaaat gagtaaactt gcattgttaa
      121 ctcatatttt ctgaatactt tcggagcaaa tttttgaatg aaatgatgca tgcaacaata
      181 gaaaaaggag catatactgc ctctgatatg gcagaaactt taattggttc ggaaatcata
      241 aaactcgctg gcgaagtaaa cgaacaaatt aagaaaggaa ataagatttt taatctgact
      301 attggcgatt atgaccctgc cgtttttcct attcccgatg cgctaaagaa tgaaattatt
      361 aaagcctatc agaatggata taccaattat cctgttgcca acggccagcc agagttgcgt
      421 aatgctgttg caggttatat caataaattg caaaaattac aatacagtag caatgagatt
      481 ttaatttccg gtggggcacg accacttatt tatgcattgt acaaaacagt tgtaaacccc
      541 ggtgagagag ttgttttccc tattccatca tggaacaata atcactattg tcatttgtcg
      601 caggcagaag ctgttgctgt agaaacacag ccacagaata attttatgcc gcttgcatca
      661 gaactcaaat cacatctgtc atcggcagca ttggttgcac tttgttctcc tctaaatcct
      721 acaggtacgg tctttagcag tagtcagtta atggaaatat gtgagttgat attgcaggaa
      781 aacaaaagaa gagaaggtaa gaagccattg tatcttatgt acgatcagat ttatatggct
      841 ttgacttttg gtaaaacaaa acatgttgac ccggtatcac tgcttcctga aatgcgacct
      901 ttcacaatat atattgatgg tctttccaaa gctttttctg caacaggttt gcgtgtaggc
      961 tgggcatttg gtccggtagg aattattgat aaaatgaaat ctattctctc gcatgtaggc
     1021 gcatggtcgc ctaaggcaga gcaggtggca acagcacatt ttctgacgca agaaaaagag
     1081 gtagatgtat ttttaagtca ttacaaatct gaagtagaaa aaagactgaa tgcattttat
     1141 gatggcttta taaaaatgaa aaatgatgga ttgcctgtca atgccattga cccgcaagca
     1201 gccatttacc tcaccgttca gtttgatatt ttaaataagg tgatgcctga tggtaaacgt
     1261 ataaataatt caaacgacat tacatcgttt ctcattaact atgccaaggt agcattagta
     1321 cccttttctg catttggtgc aacaacaact tcaaactggt ttcgactttc tatcgggact
     1381 gcccgtatgg aagatataga acagatgttt gtttctttac gcaaagcttt agccttgctc
     1441 ggtgactgac ttatctctga cccggtattg catcagactg tgaataaaaa cagaactgat
     1501 gacaatgact gcgccaatgt agaaaccgtt attgagcaat tcattttctt tgaataaaat
     1561 tattgcaaga acaatgctgt aaagcggttc taaatttact gagaggttca tggtaaaagc
     1621 actcactttt tttaatgcct ccaatgaaag tgtaaaggca atagaggtgc aaactacact
     1681 cagcaacaac agccacaccc aatctatctt tgatggaatt tgaaaactaa cattaaaagc
     1741 cggaaggatg agtggaagaa gaacagataa aaacacaaat ccactgatta attctatgaa
     1801 tgttaccggt gcaggctcgt cattgctgac aaatatttta ttcagaatag taaaaagggc
     1861 accaagcaat gcactgatta atgctagaac aatgcccatg gcatataact gctgaaaagc
     1921 aaaaatgata tagatgccaa caagtacaaa caacgctaag atgatgtttt gtttatttat
     1981 ttttttgcga taaaacaatg gctccagcaa tgcagaaaat agtgaaaccg aagcaaaaca
     2041 actaagtgca attgacacat tagaagcttt tatggaggca tagaaggtga gccaatgcag
     2101 acagactaca aaacctgtga tgatgatgga tgctaatctt tttttatcag gcagtataaa
     2161 ctgtttgtgt tttaccataa atggtatcaa tgccactgca ctgatgatca ttctgtacca
     2221 tacaatcatg atagcattca tctcaattgc cttaccgaat attccggtaa atccccacaa
     2281 caaaatggag atgtgcattt gcagataagc ctttcgcatg aactaaaaat ttattaattg
     2341 ctgataaact tttcttgttg gcaagcccat aacattataa aaacatccgt taattttttc
     2401 tatacatgtc aatccaaacc aatcctgaat tccgtaagat cctgctttgt catagggctt
     2461 aaaattacta atgtaataat gcatgtcttc tgtagtgtag ttgcgaaaat aaacttcact
     2521 cctgtcatag agcaattctt ttttgtgaat ggaagcaatg caaacgcctg tataaacttc
     2581 atgcatgttg cctttaagag tagtgagcat ttcaatagct tcgttaaagt ctgccggttt
     2641 attcagcact ttgttctgat gtacaacaat ggtatctgcg ctgatgagta attcattttc
     2701 ctgaatctca ttttcgaaag caatagcttt ttttgaagca agatataaga ctatctgttc
     2761 gttctgcaag tgttccggat aatcttcaac agtttctttt gttcttactt caaattcaat
     2821 acccatcatt tgcaaaagct gctgtctgcg tggtgaacgt gaagccagaa ttattttttg
     2881 tttgagatga ttcattgcat caggattgat gtgcaaagga aatggtttta tgcttgcatc
     2941 ggaatttata ctcttatgtg tatttactta atcaacaatt tttttaaatc ttttttgctt
     3001 ccgttaaaca cattggcatc ataagaagaa cttgaaccgg gaatagaaac gcggtcggag
     3061 tgttgccaga aaagccattt taccgacttc tcaattttta attcgtcttc gtagtagtgt
     3121 gcaatccaaa agttatagtc aggaaaatca tcagccaaat aatcttcaat aaaactgata
     3181 ttggaatata gaataggttt tactttatat tgtttttcga tgacggcaat aaaaagtttt
     3241 aattttttta ccaattcctt tttcgtaaga cggccttttt cttcaacatc aattacaggt
     3301 ggtaaatcac cactttttaa ttttacactt gaaataaaat tttttgcttg taatgaaaca
     3361 tcagcattgg gaaggaaata atgataagca ccacgaataa tattattttt ttgtgcgctc
     3421 tcccagttat caataaaggt tgggtcttca agtaaaattc cttctgtagc ttttataaaa
     3481 gcaaagctga aattaatagt atcgccattt aagtcagctg cttttagttt actaaaatca
     3541 acatctgtct gccaacgtga aacatcaata ccatgaacag tgtagccttt tggaaaactt
     3601 atttttgttg cagaaccggt ttctttatta aacaatcttt tttcaaggcg ccatgcttta
     3661 tagccataac ggattaaatg attacggtaa aaaaatgcaa gaacggctat gccacataat
     3721 agtagtgtaa ctaacagtat tctttttccg aatgattgtt tcttcgccat tgttccgtaa
     3781 cgaataacgc aaataatttt taattgcgct attgacttat acttaaaaat aaaaaatcag
     3841 gttcaccgaa cctgattttt ttatttaatg aaattaaacg gaaattattt tatcacctta
     3901 acagtcattc taatatcttg caaaggtctg ttttgaggat tggttgcttc tgctgcaatc
     3961 ttatcaagca catccatacc ggaaacaagt tcgccaaaaa ctgtgtaatc catatctaaa
     4021 aatggagtac cgcctacgga tgtatattct ttacgctgct caggcgtgta tttcatgtgt
     4081 ttggcatttt ccatcatagt cagttcattg tctgcggcag gttttccctg cacaatataa
     4141 aactgacttc cggaagatgc tttttgcgga ttaacctgat cgccaagacg tgcagctgcc
     4201 aacgcacctt ttttgtgata taatgcagta ttaaattcag caggaatagt gtatcccgga
     4261 ccaccattac ccaacatagc accggcagca gcattcttag actgcgggtc gccaccctga
     4321 atcataaaat ttttgatgac acgatgaaac aacgtgctgt cgaaaaaacc ttcattggca
     4381 agtttaatga agttgtcgcg gtgtaatggc gtttcattgt agagtttaaa tcgcattgta
     4441 cctaaattgg ttgtaatttc caccatagtt tctttgtttt gacttttttc cgcctgaagt
     4501 tttgcctggc ttttatccgt ttgtgacagt gccatcaggg gaacgattgt tgcaataata
     4561 cagatgattt ttttcatttc tatttttaat tacttttgag ctgataaaac attttacgat
     4621 accgttcgta aaaacaggta caagaataat aaatttccat tcgcatgaaa agtagtttac
     4681 gtaaaactca tttagtttta acttcattga tagtaatgac agttcttttt actttttcct
     4741 gtaaaaaaga aggaccttgc gaagctatca ttaccattac tgatagtgca ggagtagccg
     4801 ttagaggtgc aactgtaatt ttaagacagg atgctgttgt aaacccaact acaggggtgc
     4861 aggctaatgt ctatcaggaa ggtgttactg atggttatgg taaagcctct tttgagttta
     4921 agttggaggc agttttaaat attgaagcca gtaaaggccc caaactcggc aaagactatg
     4981 tacgtttgga gcaatcaaaa actgtttacc gtacggtaat catcaaataa cctcgctttt
     5041 ttcaagttca tatccatctt cctcaaaata gcggataatg ttgattttca gcaataaatc
     5101 ttgctctttt aaaggcattt gaggcattgt tttagctttt tcatactgtg gccagccatc
     5161 ggaatcaaca cctgtaaact tataataacc atcatagcta aggagtttgc aagtggctat
     5221 atgcatcaaa tcctgttttt cttccttagt aaagttctga tatccttttc ccagttcttg
     5281 tactccaatt aggaaaatca taccctgtaa atcgggcttt tcaccaaagg ttatctccaa
     5341 ttgccgtaaa agttcacccc acttatgctt gattttctga atatttgttt cgttcataaa
     5401 caataaagtg ttcaaaagta ccaatattag agactaaacc accaaacaat aaaatatcta
     5461 attttgataa accacaataa atccacaata aacaatgaac ctgttaagat taaaactggc
     5521 aattatagcc gtctttgtgg cttggttgct acctctggct gaggtttcag ctaagaatca
     5581 acctgttgac attgtcttta cacttgacct tagtggaagc accaatgggc tgatagatga
     5641 tgttcgcgat aacatctggg gaatgaataa tgaacttacc cgactttatc ctaacgctga
     5701 tattcgtttt gcagttgttg gatattcacg tccaagtttt ggcagcaaaa atcagttcgt
     5761 taaagttatt tctccattta catcaaacat tgactttata gcaacagaac tttacaaact
     5821 gaagcctaat attgaaaaag gcgatcagta tgttggtgct gcaatacgtg cttcgcttga
     5881 tatgttgagt tggtcgcatg aaaaaaatgc agtgaaacaa atttttctga caggaaatgg
     5941 ttcagtgttt ttaggcgcat ttgatgttgt tgaatcttgt gatctggcca aagaaaaagg
     6001 aataactgta aattcattgt attgctacag ttcattacgt gccaaagaaa ttagtggctg
     6061 gtataaaatt tctgaaatta ccggtggaaa gtcaattgat gttaaagtgc ataaacgtct
     6121 tcctgactat gcaacagtaa cagattttaa ccgtcttcag atgctggcaa cagaactcaa
     6181 taaaacttat atttattatg gtaaagaagg ccgtgataaa tttaaggcta tggtaagtaa
     6241 tgagaagaat gtgcttaatg cacgtcattc tgcatttgaa gatttgttat atcataaaat
     6301 ttccgatcgc tttcagggaa aacagagtga ttgggatttg gttgattatt tgaaatcaag
     6361 aaacggtaat cttaaaaatg tagatgctcg tttacttccg gactcattaa aaaatattaa
     6421 tcccgaacag ttgcttacta agctaatgat tttgaaagaa agaagaagtt atttgttaag
     6481 tcaaatacgt cagttacttc ctttcgaccg tcaggataaa ttaacagcat attttaattc
     6541 aaagcaatcg gacagcgata tgatttttga ccgtcaggtg atgatggttg taaaagatga
     6601 aatgcagtct gatttagctg caaactgatg ctcaggaatt ataattttca agtgctacat
     6661 cccagtttat caaattccac caggcattaa tataatctgc tcttttattc tgatagtgca
     6721 gatagtatgc atgttcccaa acatcaattc ctaatatagg tttaccttta acttcggcaa
     6781 tatccatcag tgtgttgtcc tgatttggag tagatgtaac gcttagtttg ccgtcagctg
     6841 atttaattaa ccaagcccaa ccggaaccaa atctgttttt tgcagcatct gaaaatgctt
     6901 tttgtaaatt atcggtacca cttaattctc taattatagc ctcagctaat tctccctttg
     6961 gtgattttgc tccaccggga cgcatcaacc tccagaaaaa agaatgattg taatgtccac
     7021 ctgcattatt gcgaattgca acagaatgtt ttgatacttt acctaacaaa tcatttaaag
     7081 acacaccttg taatttttct gcaacaactg ctttgttaag attgtttaca tatccctgat
     7141 gatgctttgt ataatgtatt tccattgtca atgtatcaat atatggctct aatgcttcaa
     7201 aaccatattc caaatcgggc aatttaaata agttgtcttc gttggtttct gatgcaatgg
     7261 caaccttgct gccaattccg tacaatgcca gcgttcctgc agccaatgcc gaagttttta
     7321 taaaatctct cctgtttttc attttcttta taattaagat aattcaatca atactgtatt
     7381 cttttcaact ttatcaccct ttaatacttt tatttttttt acaacagcat cattggtagc
     7441 tttaagattg ttttccattt tcatagcttc caatattagt aggctatcgc ctttttgaat
     7501 agcttgtcct tcggaaacta aaatatcaag taccaaaccg ggcataggtg cttttaggtc
     7561 atttactttc tgtgcaaaca ttttatccat acccaattgc tgcagtaatt catcatattt
     7621 atctttaagt gcaacctgat aaacattacc gttaactctt attttaaatt gcttttcatt
     7681 gtaatttgct tccagcaatt cagccttata cgatttgttt tgcagtagaa tacttaatga
     7741 cgattctgat atttttttga tgtcggcttc aacttgctga ttgttttttg ttaaaacttt
     7801 tttcttcgaa tcgaagttga gattaatttc ctgaccttta attattgctt tcaaaatttg
     7861 tgttttgttt ctgcaaatat aaaattctac tgctaatcgg cataatatga tatggttgac
     7921 ctgatgaaat gccatgtcat aattccggca ccgactaatt ttattcctgt tgaaataaaa
     7981 gttccgccta cggttgccaa ccccgaccgc aaggcatctc ttgtattttt tcctgcaagc
     8041 aaatcaccaa cgatggcacc aataattgtt cccataatca ttcccacagg agtaagaaaa
     8101 attccaagta acataccaat gacacttcca attacaccat aacgtgtagc acca
//
LOCUS       DAOOOI010000082         8104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1752_length_8104_cov_9.387129, whole genome shotgun sequence.
ACCESSION   DAOOOI010000082 DAOOOI010000000
VERSION     DAOOOI010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1752_length_8104_cov_9.387129"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="PKX59_09045"
     CDS             <1..1268
                     /locus_tag="PKX59_09045"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HOZ91052.1"
                     /translation="YGYYNGTRNTYQPNEYTLPDGITPLSATYYKSNTPTTINIFTLK
                     TDYSQSFFKGKLGAGYKVALVNTVNTFNFYNIEGGIATLDSSRSNKFDYTENVNAIYL
                     NYQRTFNMIDVQVGVRMENTNSNGKLKTSATANDKDVKRSYTDFFPSAGITYNINKDN
                     SAGLIYSSRIDRPNYQELNPFEYKLDELSFRKGNPFLNPQYSNKIELTHTYKYIITTS
                     AGYSHTKDFFAQITDTLGEGKSYLMPRNLATEDVLSISLSASLQPFKWYSIYLNASVD
                     NQKYKADYGGNKTINAQFTSFNLYGQNTFKLPYQFTFELSGWYNSGGVWGGSYKTKAQ
                     GSLDVGLQKKLLKDQATLKISYTDLLHTAPWDSYNTYAGIVSRAWGNWESQQLRVSFT
                     WRFGNKQMKNIRQRSSGSEQEQKRIGGGD"
     gene            1390..4008
                     /locus_tag="PKX59_09050"
     CDS             1390..4008
                     /locus_tag="PKX59_09050"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOZ91053.1"
                     /translation="MSELSKIYNPQETESKWYAHWMKEKFFKSVPDERPPYTIVIPPP
                     NVTGVLHMGHMLNNTIQDVLIRRARMLGKNACWVPGTDHASIATEAKVVQMLRDRGIK
                     KSDLTRDEFLKYAWEWKEKYGGIILEQLKKLGCSCDWDRTRFTMEDDLSEAVIDVFID
                     LYNKGYIYRGLRMVNWDPAGLTAVSDEEVIHKDVNSKLYYVRYKIDGTADEWITIATV
                     RPETILGDTAVCVHPEDERYAHLKGKFCIIPIVNRRVPIIFDDYIDKEFGTGALKVTP
                     AHDINDYNLGLKHKLEVIDTLTPDGKMSEAAGHYIGEDRFVVRKKITKDLEASGHLVK
                     TEDYKNKVGYSERTDAVIEPRLSLQWFVNMPEISKPALEAVVNGNIKLIPDKFISTYR
                     YWMENVKDWCISRQLWWGQRIPAWYDESGNFVVAKTSQEALAKFAEKNIKTNASAIKQ
                     DDDVLDTWFSSWLWPISVFDGFKDPENKDIKYYYPTNDLVTAPEILFFWVARMIIAGL
                     EYRNDIPFERVYLTGIVRDKLGRKMSKSLGNSPDPLELIEKYGADGVRVGMLLSSPAG
                     NDLLFDESYCEQGRNFANKMWNAFRLIKNWQPDTSLRQSETGRIATAWMKSKLNEQLA
                     LINDSYDKMRISEVLMIKYKLFWDDFCAWYLEMIKPAYGSVAVDTETYNTTIDIFENL
                     LQILHPFMPFLTEELWHELKNRNNRDCIIVSTWPKQSSPDNAILEGMTHAQNIISTLR
                     TFRNEKGISPKTQLQLLTKDKHQTTFDDIILKLANVSSIDKAESFDKGFAFMAGTQEY
                     AIPFDQHIDTAAERLRIEKEIAYNKGFLDTVMKKLGNANFVSKAKPEVIASEEKKKSD
                     AEQKIKSLEQQLNMMS"
     gene            4015..4230
                     /locus_tag="PKX59_09055"
     CDS             4015..4230
                     /locus_tag="PKX59_09055"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOZ91054.1"
                     /translation="MDKPAEWVKVYTTTQPHKVEIVRAVLEEHDIESMVINKNDSSYP
                     GLIGNIEIYVLDKLEVLARFIIKDNEL"
     gene            4227..5051
                     /locus_tag="PKX59_09060"
     CDS             4227..5051
                     /locus_tag="PKX59_09060"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOZ91055.1"
                     /translation="MNNFWTRAFTGAVFVAVMIAAIEFSPYTFMILFMVINLLSIIEF
                     YKLFIRNELTPRIFSGIVLSAILYATSALVASRTASAQLLFINIPMLFLIFIFELYLK
                     SEKPFENIAYTLLGVAYLTLPLALFNSMAFLPGENTGYHSGIIIGYFLILWASDTGAY
                     LSGMKFGKHKLFERHSPKKTWEGSIGGCLAALLLGYTNSLFFTQLALSEWLIVGLLIV
                     ITGTFGDLIESMLKRSLKIKDSGNILPGHGGMLDRFDGLFISIPFVFTYLALIGKV"
     assembly_gap    5073..5135
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5152..6351)
                     /locus_tag="PKX59_09065"
     CDS             complement(5152..6351)
                     /locus_tag="PKX59_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91056.1"
                     /translation="MKKISFIIFLACAISMQLQAQVITQLGQWSNNANFAVEYYQDKV
                     ITSTTSGIKFIDVSNPSNPVPTTTLASPNNFPMAITVDGSYAFFGGGMTGYFMIADIS
                     SINSPVQVGITYDINGTAYQIAVKGNYAFMPTSYDTLYAIDISNKSLPIVIDRIGMGN
                     FSSGVAIKGNYAYIGTAGGLKVVDISNPSNLNVLSNMNGVYSEVLADLPNNRLFVAKP
                     NQGFDVVDITNPASPSILFQGSGGNSGGDLVYKNGFVFQVGSADVSAFQIGVSSATYL
                     TSFNSTVSGQVNAICAKDSVFYLSTVNNLHVLKLSYLTGMEDNSPLETAIFYLNPAEG
                     VISLKFTSSPPSNIKLYDIMGKLVKQFNSNFSKNLQIGIADLNSGIYFVSFDESYLER
                     RIKFVKR"
     gene            complement(6403..6822)
                     /locus_tag="PKX59_09070"
     CDS             complement(6403..6822)
                     /locus_tag="PKX59_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOZ91057.1"
                     /translation="MTHLISIKNRMAIKEYTLLFFRIGVSLLMLYGHGYPKVQKLFSG
                     TEIQFADPFGLGMKLSFYLVVFAEVICSIFIILGLKTRMAASVLVFNMLVAVFVMATN
                     GEVPELALMYFISYVLILVFGGGKYSIDFNLNSKKKR"
     gene            complement(7008..7079)
                     /locus_tag="PKX59_09075"
     tRNA            complement(7008..7079)
                     /locus_tag="PKX59_09075"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7045..7047),aa:Met,seq:cat)
     gene            complement(7136..>8104)
                     /locus_tag="PKX59_09080"
     CDS             complement(7136..>8104)
                     /locus_tag="PKX59_09080"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HOZ91058.1"
                     /translation="FNEQAYSDIENIFHARGTAVTETNKRQAELPAACIPIYNKKGTA
                     PGMWFNHGEQVFVSMPGVPFEMQAMMQNDVLPKLRQLYESMPLVHKTILTQGIGESDL
                     SDRIAAWENDLPAHLKLAYLPAAGMVRLRLTGKGVEHMEQDMNLRIDKLKELIAPYIF
                     GYDDDTIQSVVGKLLTEKQKTLSVAESCTGGFLAHLITSIPGSSTYFMGGTVSYSNAA
                     KMSQLHVSEQSLKEYGAVSEAVAIEMAIGARKTYNTDFALSTTGIAGPGGGSLEKPIG
                     TVWIGVATHDACFAKKFMLGTNRLRIIQVAADTALNMLRKKINEHN"
BASE COUNT         2706 a         1484 c         1460 g         2391 t
ORIGIN      
        1 attacggcta ctacaacgga acaagaaaca cgtatcaacc aaatgaatac acattgcctg
       61 atggaatcac accactttct gcgacatatt acaagtcaaa tacaccaaca actatcaata
      121 tctttacttt aaaaacagat tattcgcaat cgttttttaa gggcaagtta ggagccggat
      181 ataaggttgc tttggtaaac actgtcaaca catttaattt ttacaacatt gaaggtggta
      241 ttgcaacatt ggattcttca cgtagcaata aatttgatta tacagaaaat gtgaatgcca
      301 tttatttaaa ttaccaacgc acattcaata tgattgatgt gcaagtaggt gtccgcatgg
      361 agaatacgaa tagtaatggc aaattaaaaa cctctgccac cgcaaatgat aaagatgtga
      421 aacgaagcta tactgacttt ttccccagcg caggcatcac ctacaatatc aacaaggata
      481 attctgcagg attaatttac agttcgcgaa ttgacagacc aaattatcag gagttaaatc
      541 cgtttgaata taagcttgat gaattaagtt tcagaaaagg aaatccattt ttaaacccac
      601 aatattcaaa taaaattgaa cttacacaca cttataaata tatcattaca acatcggccg
      661 gatatagtca cacaaaagac ttttttgcac aaattactga cacacttggc gaaggaaaaa
      721 gctatttaat gcctcgcaat ctggctacag aagatgtgtt gagcattagt ttaagtgcat
      781 cattacaacc tttcaaatgg tacagtattt atttgaatgc aagcgttgac aatcaaaaat
      841 acaaagccga ttatggaggt aataaaacca tcaatgcaca gtttacctca tttaatttat
      901 atggtcaaaa cacatttaag ttaccctatc agtttacatt tgaactttcg ggatggtaca
      961 actcaggagg tgtttggggt ggctcataca aaacaaaggc acagggatca ttagatgtgg
     1021 gcctacagaa aaaattattg aaagatcagg caacattaaa aatttcatat accgacttac
     1081 tccatacagc tccatgggac agttacaaca cttatgcagg cattgtgagt cgtgcatggg
     1141 gcaattggga aagccagcaa ctcagggtgt cgtttacctg gcgatttggc aacaaacaaa
     1201 tgaaaaacat cagacaacga agcagtggta gtgagcagga acaaaaaaga attggaggag
     1261 gcgattagca aagtcctcct tttacagaaa gaaagtacac ttatttttca aagcctgctt
     1321 ttatagcagg ctttgttcgt tttgcacata gctaatttct tatttttgca ttcacacttt
     1381 ttaaagcaca tgtcagaatt atcaaaaata tataacccac aggaaacgga atccaaatgg
     1441 tatgcacatt ggatgaaaga aaaatttttt aaatcggttc ctgatgagcg tccgccctac
     1501 actattgtta ttcctccacc aaatgtaaca ggagtgttgc acatgggtca catgctcaac
     1561 aacaccatac aggatgtact gatccgcaga gcacgcatgt taggtaaaaa tgcatgctgg
     1621 gttccgggaa cagatcatgc ttctattgca acagaagcta aggttgtgca aatgttgcgc
     1681 gacagaggaa ttaaaaaatc tgaccttaca agagatgagt tcttaaaata tgcctgggag
     1741 tggaaagaaa aatatggcgg aattattctt gagcaattaa aaaaattagg ttgcagttgt
     1801 gattgggatc gcacacgatt tacaatggaa gacgatttaa gtgaagcggt aattgatgta
     1861 tttattgact tgtacaacaa gggttacatt tatcgtgggc ttcgcatggt gaactgggat
     1921 ccggcaggat taactgcagt aagtgatgaa gaggtaatac ataaagatgt taactctaaa
     1981 ctttactatg taagatataa aattgatggc actgctgatg aatggattac tattgcaaca
     2041 gttcggcctg aaacaatttt aggcgacact gccgtttgtg tacatccgga agatgaacgt
     2101 tacgcacatt taaaaggaaa attctgtatt attccaattg tgaatagaag agtacccata
     2161 atttttgatg actatataga caaggaattt ggtaccggtg cacttaaagt aactcctgca
     2221 catgacatca acgactataa tcttggatta aaacataagc tcgaagtaat tgacacactc
     2281 acacccgatg gcaagatgag tgaagcagca gggcattata ttggagagga caggtttgtt
     2341 gtaagaaaaa aaattacaaa agaccttgaa gcaagtggtc atttagttaa gacagaagat
     2401 tataaaaaca aagtaggata cagcgaacgt actgatgcag ttattgaacc tcgcctgagt
     2461 ttacaatggt ttgtgaatat gccggaaatc agcaagcctg cattagaagc agtggttaat
     2521 ggcaacatca aacttattcc tgataaattt atcagcacct atcgctactg gatggagaat
     2581 gttaaagact ggtgtatatc acgtcagcta tggtggggac aacgtattcc tgcatggtat
     2641 gatgaatcag gaaactttgt ggtagccaaa acttcgcagg aggcattggc gaagtttgca
     2701 gaaaaaaaca taaaaacaaa tgcatcggca ataaaacagg atgatgatgt tttagacaca
     2761 tggttttctt catggctgtg gccaataagt gtttttgatg gatttaaaga tcctgagaat
     2821 aaagacatca aatactacta ccctaccaac gatttggtga cagcaccgga aattcttttc
     2881 ttttgggtgg cacgaatgat tattgcggga ttagaatatc gaaatgacat tccttttgag
     2941 cgtgtttatc ttaccggaat tgttcgtgat aagttaggca gaaaaatgag taagtcatta
     3001 ggtaattcac ccgacccact tgagctaata gaaaaatatg gtgcagatgg tgttcgtgta
     3061 ggtatgttgc tttcttcacc tgccggtaac gacctattat ttgatgaatc atactgtgag
     3121 caagggcgca attttgccaa caaaatgtgg aatgcatttc gtctgataaa aaactggcag
     3181 cctgacacat cactgcgtca atccgaaaca ggaagaattg caacagcatg gatgaaatca
     3241 aaactcaatg aacaattagc actcatcaac gacagttatg ataaaatgcg catcagtgaa
     3301 gtgcttatga ttaaatacaa gttgttttgg gatgatttct gtgcatggta tttagaaatg
     3361 attaaacccg cttacggaag cgtagccgtt gatacagaaa cctacaatac aactattgat
     3421 atttttgaaa acctgctaca aatactgcat ccatttatgc catttttaac agaagagttg
     3481 tggcatgaac ttaaaaacag aaataacaga gattgcatta ttgtaagtac atggccaaag
     3541 caatcatcac cggataatgc tatattggaa gggatgacac atgcacaaaa tataatttct
     3601 actttaagaa cttttcgaaa cgaaaaaggg atatcaccaa aaactcagtt acagttattg
     3661 acaaaagata aacatcaaac aacgtttgat gacatcatac ttaaattagc caatgtcagt
     3721 agcattgata aagcagaaag ttttgataaa ggatttgctt ttatggcagg cacacaggaa
     3781 tatgctatcc cttttgatca gcatattgat actgctgcag aacgtctccg aattgaaaaa
     3841 gaaattgcat acaacaaagg ctttcttgat acggtaatga aaaaattagg taatgcaaac
     3901 tttgtaagca aagccaagcc tgaagtaatt gcaagtgaag agaaaaagaa atcggatgca
     3961 gaacaaaaaa taaaatcatt agagcaacaa cttaatatga tgagctaact gaatatggac
     4021 aagccggcag aatgggtcaa ggtatatact acaacacaac cacacaaagt agaaattgtg
     4081 cgtgctgtgt tagaagagca tgatattgaa agtatggtaa tcaataaaaa tgattcaagt
     4141 tatccgggat taattggcaa tattgaaata tatgtactcg ataaactgga agtgcttgca
     4201 cggtttatta taaaagacaa cgaactttga ataacttttg gacaagagca tttacgggtg
     4261 ctgtatttgt agcagtgatg attgctgcca ttgaattcag cccttataca ttcatgatac
     4321 ttttcatggt catcaacctg ttaagcatca ttgagtttta caaactgttt atccgaaacg
     4381 aacttacgcc aagaattttt tcaggcattg tactaagtgc gattctttat gcgaccagtg
     4441 cattggtagc ttcgaggacg gcatctgcgc agttattgtt tataaacatt cctatgcttt
     4501 ttcttatttt catttttgag ttatatctca aatctgaaaa accatttgaa aatatagctt
     4561 atacgttgtt gggtgtagct tatctgacat tacctcttgc actatttaac tctatggcat
     4621 ttttacccgg tgaaaataca ggctatcata gtggaattat tattggctat ttcttaattc
     4681 tatgggcaag tgacaccgga gcctatcttt cgggaatgaa atttggaaag cataaacttt
     4741 tcgagcgtca ttctcctaaa aagacctggg aaggcagtat tggcggttgt cttgctgcat
     4801 tacttttagg ttatactaat tctttgtttt ttacacagct tgctttatca gaatggctga
     4861 ttgttggttt gcttattgta attacaggaa cttttggcga tttgattgaa agtatgctga
     4921 aacgtagcct taaaatcaaa gactcaggaa acattcttcc cgggcatgga ggtatgctcg
     4981 atcgctttga tggactattt atttctattc catttgtttt tacttacctg gctttgattg
     5041 ggaaggtata ataagtaacc tttaattaac ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagatg aaaaccttcc attacctctt
     5161 tacaaacttt attcttcttt caagataaga ttcatcaaaa gaaacaaaat aaattcctga
     5221 attcaagtca gctatcccga tttgcaaatt ttttgaaaaa ttagaattaa attgcttcac
     5281 taattttccc attatatcat acaacttaat attggatgga ggactggagg taaatttcaa
     5341 tgagatgaca ccctcagcag gattaaggta aaaaattgct gtctctaatg gactattatc
     5401 ttccatgcct gtgaggtagc ttagcttcag aacatgtaaa ttattaactg ttgataaata
     5461 aaaaactgaa tcttttgcac aaattgcatt tacctgacca ctcactgtac tattaaacga
     5521 agtcaaatat gttgcagagg aaactcctat ttgaaatgca ctaacatcag cagatcctac
     5581 ttgaaataca aatccattct tataaactaa atctccaccg gaatttccac ctgaaccctg
     5641 aaaaagaatt gatggacttg ctggattagt aatgtcaacg acatcgaaac cctgatttgg
     5701 ttttgccaca aataatctgt tatttggcaa atcagcaaga acttcagaat aaacaccatt
     5761 catatttgac aacacattta agtttgaagg atttgaaatg tcaactacct ttaaacctcc
     5821 agctgttcca atatatgcat aattaccttt tatagcaaca ccagaagaaa agtttcccat
     5881 accaattctg tcaataacaa taggtaaact tttattgcta atatcaattg catataaagt
     5941 atcgtaactt gtaggcataa aagcataatt acctttaact gctatctgat aagctgtacc
     6001 attaatatca taagttattc ctacctgaac cggtgaatta attgatgaaa tatctgcaat
     6061 cataaaatag cctgtcatac ctccgccaaa aaacgcataa ctcccatcca ctgtaattgc
     6121 cattgggaag ttattaggag atgctaaggt tgtagtagga actggattcg atgggttcga
     6181 cacatcaata aatttgatgc ctgaagtggt gctggtgata actttatcct gataatattc
     6241 aactgcaaaa ttggcattat tactccattg accaagttgg gtaattacct gagcttgtaa
     6301 ttgcatacta attgcacaag ccaaaaaaat aataaaactt atctttttca tgattttgaa
     6361 atttttattc aacatttaaa aataaattaa aatcaactat gctcaccttt tcttctttga
     6421 attaagatta aaatcaatag agtatttacc tccgccaaaa actaaaatca gtacataaga
     6481 gataaaatac atgagtgcca attcaggaac ttctccatta gtagccatga cgaaaactgc
     6541 aacaagcata ttaaaaacca atacagatgc agccatacgt gtttttagtc cgagtatgat
     6601 aaatatagaa caaatcactt cagcaaaaac taccagataa aaagaaagct tcatgccaag
     6661 cccaaacgga tcggcaaatt gaatttctgt tccactaaat aatttctgta ctttaggata
     6721 gccgtgacca taaagcatca gcaatgaaac tcctattctg aaaaacagca aagtatattc
     6781 ctttatggcc attcgatttt taatgctaat taaatgtgtc atgcctatac taattttgtt
     6841 tctccaaaaa taaaattttc cttggatttc aatcaatacc acaaaatcag acacaatcgg
     6901 caatatttca actcagattg aagaatgaac ttgcgttttg aaaatctata tatcaataaa
     6961 gtaaaaggaa ataaaaaagc tgcactctgt gcagcttaat cttttagtag cgggggctgg
     7021 attcgaacca acgacctttg ggttatgagc ccaacgagct acccctgctc caccccgcat
     7081 tgttattttg agggtgcaaa agtaatcact ttgtccttat ctgcaaatat attcctcaat
     7141 tatgctcatt tattttcttt ctgagcatgt ttaatgcagt atccgcagct acctgaatga
     7201 ttcgcagacg gtttgtaccc aacataaact ttttagcaaa acaagcatcg tgtgtagcaa
     7261 ctccaatcca tacagtgcca atcggttttt ccaaacttcc tccaccaggc cctgcaatgc
     7321 ctgttgtact caatgcaaaa tctgtattat aagtttttct tgcacctatt gccatttcta
     7381 ttgcaaccgc ttcactgacg gcaccatatt ccttcaatga ttgttcgctg acatgcaact
     7441 gactcatttt tgctgcatta ctgtaactca ctgtaccgcc cataaagtat gttgaactac
     7501 cggggattga agtaataaga tgtgccaaaa atcctccagt acaactttct gcaactgaaa
     7561 gagttttttg tttctcagtt aaaagttttc ctacaacaga ttgaattgtg tcatcatcat
     7621 aaccaaaaat ataaggcgca attaattctt tgagtttatc aatcctcagg ttcatgtctt
     7681 gctccatgtg ttcaacacct ttacctgtaa gtcgtaaacg aaccattcct gcagcaggca
     7741 gataggctaa ttttaaatga gcaggcaagt cgttttccca tgctgcaatc ctatctgaca
     7801 aatcgctctc tcctatacct tgcgtaagaa ttgttttatg tactaaaggc attgactcat
     7861 atagctgcct taatttagga agtacatcat tctgcatcat tgcctgcatc tcgaatggca
     7921 cacccggcat gctcacaaaa acctgctcgc catgattgaa ccacattccc ggagcagttc
     7981 ctttcttatt gtaaattgga atacatgcag caggcaattc ggcctgccgt ttgtttgttt
     8041 ctgtaactgc cgtacctctt gcatgaaaaa tattttcaat atcactatat gcctgttcgt
     8101 taaa
//
LOCUS       DAOOOI010000083         8057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1765_length_8057_cov_13.340915, whole genome shotgun sequence.
ACCESSION   DAOOOI010000083 DAOOOI010000000
VERSION     DAOOOI010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8057
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1765_length_8057_cov_13.340915"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PKX59_09085"
     CDS             complement(<1..314)
                     /locus_tag="PKX59_09085"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOZ91059.1"
                     /translation="MIFTDTHTHLYATEFDADRKELMQQAIDAGVSRFFLPNVEKSSV
                     NEMLELCKQFPENCFPMMGLHPCSVKEDYLAELALVESELSKGKYFGVGEIGLDFYWD
                     KS"
     gene            401..1399
                     /locus_tag="PKX59_09090"
     CDS             401..1399
                     /locus_tag="PKX59_09090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOZ91060.1"
                     /translation="MSRVLVIRFSSIGDIVLTTPVLRCIKKQLPNAEVHFLTKRDFSD
                     VLANNPYIDNTFYVDNNLPEIIRQLKMVGYDYIVDLHHNMRSWKVKRAVRAKKFSFNK
                     LNIEKWIYVNFKINKLPHKHIVDRYFDAISPMGVVNDGQGLDYFINPLDEVDISTFLP
                     ASFRQGYVAFVVGAKHATKCLPLEKMKEIISGLKHQVVLLGGFEDKQKGAELQKLFPE
                     KVFNACGITSIGQSASLIKQSKAVITHDTGLMHIAAALRKKIVSVWGNTVPEFGMYPY
                     LPVNSVPFSISEVKNLNCRPCSKIGYNTCPKKHFRCMNDMDIQEIIKNTQHYFETK"
     gene            1494..2366
                     /locus_tag="PKX59_09095"
     CDS             1494..2366
                     /locus_tag="PKX59_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOZ91061.1"
                     /translation="MSEDNKLARKALRSSNISTVISISLVLFLIGSLGLLLINAKQLS
                     DYLKENVILTVMMKQEASDADIQNLKKQLEGNAFIKEVSYISSEQAAEQLKQELGEDF
                     NTFLGFNPLLPLMEIKLKPDYTEENNISQLKDFISKNSIVKEVYFQESLLDAINKNIR
                     NISLIILGFSAVLFFIAGALINNTIRLALYAKRMLIKSMKLVGATRNFIRKPFIVRGI
                     LQGLIGAVIANIMILVVFYYAKKQIPEIIAFENPVNLSIVLGIVVFLGIFISGFSTYF
                     AVNKYLRQRTEDIF"
     gene            2379..2657
                     /locus_tag="PKX59_09100"
     CDS             2379..2657
                     /locus_tag="PKX59_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOZ91062.1"
                     /translation="MAKEKTITKSTTAKSASKPEIQLAFGKINYILMISGIVLILFGY
                     MLMSGGGSDDPKVFNPAIFDTQRLTVAPIMVVLGYALEVLAIVVNAKD"
     gene            2679..3479
                     /locus_tag="PKX59_09105"
     CDS             2679..3479
                     /locus_tag="PKX59_09105"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOZ91063.1"
                     /translation="MNTLQAIILAIIEGITEFLPVSSTGHMIIGSSVMGIAELPITKT
                     FTVAIQFGAILSVVFLYWRKFLVIDFNFYKKLLIAFIPAAVLGKLLDDYIDALLENVL
                     VVAAMLLVGGIIFILLDKWFQKTEERGSEELTKDKSAFYIGLFQCIAMIPGVSRSAAT
                     IIGGLAQGLNKKAAAEFSFFLAVPTMFAATLYKMYGFYKLNGGFTAEEIKLFAVGNVV
                     AFIVAAIAIKSFINYLTKHGFKIFGWYRIIIGAAILLLHFAGIELKML"
     gene            3485..4195
                     /gene="truB"
                     /locus_tag="PKX59_09110"
     CDS             3485..4195
                     /gene="truB"
                     /locus_tag="PKX59_09110"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOZ91064.1"
                     /translation="MPDYLQGEVLLVDKPLEWTSFDVVNKLRGTLQHLKFEDGSRPYK
                     KIKVGHAGTLDPLATGLLIVCTGKATKSIDSLMGLEKEYTGTFTLGQTTPSYDAETTV
                     DRTFDISHITTELIHQAAQTFVGETEQLPPAHSAVIIQGERAYEKARRGEEVKVTPRK
                     INIQSFEITAIAMPVVSFRVVCSKGTYIRSLANDFGKALNSGAYLSSLIRTCIGDFKL
                     SNAHSVEHYVEAIRSLQK"
     gene            complement(4301..5011)
                     /gene="pyrH"
                     /locus_tag="PKX59_09115"
     CDS             complement(4301..5011)
                     /gene="pyrH"
                     /locus_tag="PKX59_09115"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOZ91065.1"
                     /translation="MKYKRILLKLSGEALMGEKNFGIDNNRLTQYAEEIKTIVDKGVQ
                     VAIVIGGGNIFRGLQAAEGGMDRVQGDYMGMLATVINSMALQGALEHKGINTRLQSAI
                     TMDAICEPFIRRRAVRHLEKNRVVIFGAGTGNPYFTTDTAASLRAIEVEADVILKGTR
                     VDGIYTSDPEKNSDAVRFEHLTFDDVYEKDLKVMDMTAFTLCHENKLPIIVFDMNTPG
                     NLLRLVEGNHVGTLVDVE"
     gene            complement(5048..5953)
                     /gene="deoC"
                     /locus_tag="PKX59_09120"
     CDS             complement(5048..5953)
                     /gene="deoC"
                     /locus_tag="PKX59_09120"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOZ91066.1"
                     /translation="MAKADLRFLNHLPPVDQVGVEERVARLNKRSIKNESKMEALKLA
                     ISMMDLTTLDAKDTPGKVRQLCAKAIRPHEMEGIPSAAAVCVYANLVSVAKQALEGTK
                     VKVAAVSTAFPSGMSNRKFKLDETRYAVAEGADEIDMVISRGEFLRGEYNFVYDEIAA
                     VKEACGNAHLKVILETGELSTLDNVRKASELSIAAGADFIKTSTGKVQPAATLPVTLV
                     MLECIRDYYYRTGIKIGMKPAGGIATAKLALQYLVMVRETLGQEWLNADMFRFGASSL
                     ANDLLMQIIKQLTGAYQSADYFSKD"
     gene            6094..7440
                     /gene="purB"
                     /locus_tag="PKX59_09125"
     CDS             6094..7440
                     /gene="purB"
                     /locus_tag="PKX59_09125"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOZ91067.1"
                     /translation="MSDALFAVSPVDGRYAAVCSPLKEYFSEYALIRERVKVEVKYLI
                     ALAQLQLPQLHIDSGKFEALRNIYRQFTPADAAQIKQTEKTTNHDVKAVEYFVKEKLE
                     TLGLNNAKEFVHFGLTSQDINNTATPMLVRHGIAEVMLPALTDVLNKIESQAQEWNNI
                     SLLAHTHGQPASPTRLGKEMKVFSERLRSQLDMLKSIPHSAKFGGATGNFNAHAVAYP
                     AVDWISFADDFVNSLGLTRTTFTTQIEPYDNLSALFDNLKRINNILLDLCRDVWSYIS
                     MNYFSQTVKAGEIGSSAMPHKVNPIDFENAEGNLGIANALFEHLSAKLPISRLQRDLT
                     DSTVLRNTGVPFAHTLIALKSLLKGLNKISPNTQVISADLNRHAVVIAEGIQTILRRE
                     GYPNPYEALKDLTRGKENITLQDIHQFINTLQVGDTVKAELLKITPENYTGVNWSD"
     gene            complement(7432..8028)
                     /locus_tag="PKX59_09130"
     CDS             complement(7432..8028)
                     /locus_tag="PKX59_09130"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOZ91068.1"
                     /translation="MFTGIIETLGIVKKIENEGSNRHFTVSCSFADELKIDQSVAHNG
                     VCLTVVNINHENKTYKVTAVDETLRKTNLSSLVEGNEVNLERGMLLNTRLDGHIVQGH
                     VDGTAVCTSITDENGSWKFIFENDGSARNLIVEKGSVTVNGVSLTVVDAGDYMFSVAI
                     IPFTMQHTTFKNLKRGQTVNVEFDIIGKYVARLTEKSV"
BASE COUNT         2468 a         1625 c         1452 g         2512 t
ORIGIN      
        1 aaacttttat cccagtaaaa gtcaagacca atttctccta caccaaaata ttttcccttt
       61 gacaattcag actcaaccaa agccaactca gccaaataat cttcttttac agagcatgga
      121 tgcaacccca tcataggaaa acaattctcc ggaaactgtt tgcacaattc aagcatttcg
      181 ttaacacttg atttttcaac attaggaaga aaaaaacgac tcacaccggc atcaatagcc
      241 tgttgcataa gctctttcct gtctgcatca aactcagtgg catagagatg tgtgtgtgta
      301 tctgtaaaaa tcatgatgca aagtaaatcg aatttaagta tttaaaccaa tgcttacctt
      361 tgcaaacaat ggcattagac attttttcga aaaccactgt atgtcccgag ttttagtcat
      421 ccgttttagc tctattggcg atattgtttt aacaacacct gttttgcgtt gtattaaaaa
      481 acaattgccc aatgcagaag tgcattttct taccaagcgc gattttagtg atgtattggc
      541 aaacaacccc tacattgaca atacttttta tgttgacaat aaccttcctg aaattatcag
      601 gcagctaaaa atggttggct atgattatat tgttgacctg catcacaaca tgcgttcatg
      661 gaaagtgaaa agagcagtaa gagcaaaaaa attcagtttt aacaagctta atattgaaaa
      721 gtggatttac gtcaatttta aaataaataa gttgccgcat aaacatattg ttgaccgtta
      781 ttttgatgcc atcagcccaa tgggtgtggt gaatgatggg cagggtcttg actatttcat
      841 taacccgctt gatgaagttg acatttctac ttttttacct gcatcattcc gtcagggata
      901 tgtggccttt gttgtgggtg ccaaacatgc tactaaatgt ctgccacttg aaaaaatgaa
      961 agaaattatc agcggactga agcatcaggt tgttttgctt ggcggatttg aagacaaaca
     1021 gaaaggagca gagcttcaga aattatttcc cgaaaaagtt tttaatgcct gcggcataac
     1081 aagtattggc cagtcggcat cactcatcaa acaaagtaaa gccgtaatca cacatgacac
     1141 cggactgatg catattgctg cagccttgcg aaaaaaaatt gtttccgttt ggggaaacac
     1201 cgtccctgag tttggaatgt atccctatct gcctgtaaac agtgtgcctt tcagcatcag
     1261 cgaagtgaag aatttaaact gcaggccttg ttctaaaatt ggttacaaca cctgtcctaa
     1321 aaaacatttc cgctgtatga acgatatgga tattcaggaa atcataaaga acacacagca
     1381 ttattttgaa accaaataat ccgatgccga taattaaaat ttattaataa ccctgttgaa
     1441 taaattctgt ttcaggttat tgatgcagtg ttttaccatc taaatttgct gccatgagtg
     1501 aagataataa actggcaagg aaggcacttc gcagttcaaa tatttcaacc gtaattagta
     1561 tttcattagt attgtttctg attggttcat tggggctgtt actcatcaat gccaaacaac
     1621 tttctgatta cttaaaagaa aatgtaatct taacggtgat gatgaagcag gaagcctctg
     1681 atgccgacat tcagaatctt aaaaaacaat tagaaggaaa tgcttttatt aaagaagtat
     1741 catacatcag cagcgaacag gcggcagagc aattaaaaca agaactggga gaagatttca
     1801 atactttctt agggtttaat ccattgttgc cactgatgga aataaaactt aagcccgact
     1861 atactgaaga gaataatatt tcgcagctaa aagattttat ttctaaaaac agtatagtta
     1921 aggaagttta ttttcaggag tcgttgctcg atgccatcaa taaaaatatc agaaatatca
     1981 gcttgattat tcttggattc agtgctgtgt tgttctttat tgctggtgca ttaatcaaca
     2041 acactatcag gcttgcgctc tatgctaaac gtatgctcat aaaaagtatg aagttagttg
     2101 gggccacaag aaattttatc agaaaaccat ttattgtaag aggaattttg caaggtctga
     2161 ttggcgcagt cattgccaat attatgattt tagtagtgtt ttactatgcc aaaaagcaga
     2221 ttcctgaaat catagcattt gaaaatccgg tgaatttgag catagtttta ggcattgtgg
     2281 tgtttttagg catttttatt tcaggattca gcacctattt tgcagtaaat aaatatcttc
     2341 gccaacgaac tgaagacatc ttttaacaat tcaaattcat ggcaaaggaa aaaaccataa
     2401 ccaaaagtac aacagcaaag tctgcatcaa agccggaaat acaattagcc tttggaaaaa
     2461 taaattacat cctgatgatt tcgggcatag tacttattct gttcggctat atgctgatga
     2521 gtggtggcgg ttctgacgat ccaaaagttt ttaatcctgc catttttgat acacaacgat
     2581 taacagttgc gccaataatg gtagtgcttg gctatgccct tgaagtactt gccattgttg
     2641 tgaatgcaaa agattaaaac actcttccaa ctattttaat gaatacactg caggctatca
     2701 ttctggccat cattgaaggt atcacagagt ttctgcctgt atcttctacc ggacacatga
     2761 ttatcggctc ctccgtaatg ggcattgccg aacttcccat cactaaaaca tttactgttg
     2821 ccattcagtt tggcgcaatt ttatctgtgg tatttttata ctggcgtaag tttttagtta
     2881 ttgattttaa tttttataaa aagttactga ttgcatttat tcctgctgct gttttaggca
     2941 agctgcttga cgactacatt gatgctttgc tcgaaaatgt attggtggtt gcagccatgt
     3001 tgcttgttgg aggtatcata ttcattttgc ttgacaaatg gtttcagaaa acagaagaac
     3061 gcggaagcga agaactcaca aaagataaat ctgcttttta tataggattg tttcagtgca
     3121 ttgccatgat tccgggagta tcgcgctcgg cagctaccat cattggcggc ttagctcagg
     3181 gactcaataa aaaagctgct gcagaatttt cgttcttcct tgccgtgcca actatgtttg
     3241 cagctacact atataaaatg tatggcttct ataaactgaa tggaggtttc accgcagaag
     3301 aaataaaatt atttgctgtg ggaaatgttg ttgcatttat tgtagctgcc attgccataa
     3361 aatcatttat aaattatctg accaagcatg gatttaaaat tttcggatgg tacagaataa
     3421 ttattggtgc tgctattttg ctgttgcact ttgcaggcat tgaacttaaa atgctttaat
     3481 ctgcatgcct gattatttac aaggtgaagt gctgttggta gataaacctt tagagtggac
     3541 atcttttgat gtagtgaata aattaagagg caccttacag catctgaaat ttgaagacgg
     3601 cagcagacct tataaaaaaa taaaagttgg acatgccgga actttagacc cactagcaac
     3661 gggtttgctt attgtttgca ccggtaaggc cacaaaaagt attgactcac taatgggtct
     3721 tgaaaaagaa tatacaggca cttttactct tggacaaact acaccatcgt atgatgcaga
     3781 gacaacagtt gacaggacat ttgatatatc gcacatcact acggagctaa tccatcaggc
     3841 agcacaaact tttgttggag aaacagagca actgccacct gcacattctg ccgtaattat
     3901 tcagggtgaa cgtgcttatg aaaaagcaag acgtggcgaa gaagtaaaag ttacaccacg
     3961 aaaaattaat atacaaagtt ttgagattac agcaatagct atgcctgttg tttcatttcg
     4021 tgttgtatgc agtaaaggaa cctacatacg ttctttagcc aacgattttg ggaaagcatt
     4081 aaactccggt gcatatctga gctcactcat ccgcacatgc attggtgatt ttaaacttag
     4141 caatgcacac agtgttgaac attatgttga agcaataaga agtttgcaga agtagttttt
     4201 ttagggggtg gctagttttg aaatgaatca ttctcctttt agtatttcat tatcaaaaaa
     4261 atcttttcga aattctattc accaaaaatc ttcttgcaat ttattccaca tcaaccagcg
     4321 tgcctacatg attgccttct acaagtcgca acagattgcc cggtgtattc atgtcaaaga
     4381 ctatgatagg aagtttgttt tcatgacaca gtgtaaatgc agtcatatcc ataaccttta
     4441 aatctttttc gtaaacatca tcaaaggtca gatgttcaaa gcgcactgca tcgctattct
     4501 tctcaggatc ggatgtgtag atgccatcaa cccttgttcc tttaagaatt acatcggctt
     4561 ctacttctat ggcacgtaat gatgctgctg tatctgttgt aaaataggga ttaccagttc
     4621 ctgcaccaaa aatcacaact ctgttttttt caaggtggcg cactgccctt cttcttataa
     4681 aaggctcaca gatggcatcc atagtaatgg cagattgcaa acgcgtgttt atgcctttat
     4741 gttccaatgc accttgcaaa gccatactat taattactgt tgccagcata cccatgtaat
     4801 cgccctgcac cctgtccatg ccgccttctg ctgcctgcaa ccctctgaaa atattacctc
     4861 cgccaataac tattgcaacc tgaacacctt tgtcaacaat tgtttttatc tcctctgcat
     4921 attgggtaag cctattgttg tctatcccaa aatttttctc tcccattaat gcttcaccgc
     4981 taagtttcag taatattctt ttatacttca tatcagattt gtgtttttat ctttttagtt
     5041 ttttctttta atcttttgaa aaataatcgg cactttgata ggctcctgta agctgcttta
     5101 ttatctgcat cagtaaatcg ttggctaacg agcttgcacc aaacctgaac atatcggcat
     5161 tcaaccactc ctgccctaat gtttctctaa ccatcactag atattgtaat gccaactttg
     5221 cagtagcaat gcctcctgca ggtttcatcc cgatttttat tcctgttcta tagtaataat
     5281 cacgaataca ttcaagcatc accaatgtta ccggcaatgt tgctgcaggc tgcaccttgc
     5341 ctgttgatgt ttttataaaa tctgcacctg ccgcaataga taactcactt gcttttctca
     5401 cattatccaa tgttgaaagt tctcctgttt ccaaaatcac tttcagatgt gcattgccac
     5461 aggcttcttt tacagcagca atttcatcat aaacaaaatt atattctcct cttaaaaatt
     5521 caccacgcga aataaccatg tcaatttcat cggcaccttc tgcaacagca tagcgtgttt
     5581 catcaagttt aaattttcta ttgctcatgc cgctgggaaa tgcagtggac actgctgcca
     5641 cttttacttt tgttccctcc aatgcctgct tggcaacaga aaccaaatta gcataaacac
     5701 atacagcggc agcagaagga attccttcca tctcatgtgg tctgatggct ttggcacaaa
     5761 gctgtctcac ttttcctggt gtgtcttttg catcgagtgt ggtgaggtcc atcatcgaaa
     5821 tggctaactt caacgcttcc atcttcgact cattttttat gctgcgcttg tttaatcgtg
     5881 caacacgctc ttctacacca acctgatcta caggtggcag gtggttgaga aaacgtaaat
     5941 ctgcttttgc catgctgata agataaaatt tcagagagct aaaataaaca ttccatatca
     6001 attgcagtaa aatttattgt cgtaggatat aatccaaatc ataaacagca tttaattttt
     6061 aaaatctacc tttgcagcac caaatattat gctatgtctg atgctctttt tgccgtttcg
     6121 cctgttgacg gacgctatgc cgctgtttgt agtccgttaa aagaatattt ttctgaatat
     6181 gcgctaatcc gtgagcgtgt taaagtagaa gttaagtatc ttattgcact ggcacaactg
     6241 caattgccac aacttcatat tgattccgga aaatttgagg cacttcgcaa catctaccgt
     6301 cagtttacac cggccgatgc agcacagatt aagcagacag aaaaaacaac caatcatgat
     6361 gtaaaagctg ttgagtattt tgtaaaagaa aaattagaaa cattaggcct gaacaatgcc
     6421 aaagagtttg ttcacttcgg acttacctcg caagacatca acaacactgc aacacccatg
     6481 cttgtccgtc atggtattgc cgaagtaatg ctgcctgcat taacagatgt gttaaacaaa
     6541 attgaaagtc aggcgcagga atggaacaac atcagcctgc tggctcatac acacggacag
     6601 ccagcaagcc caacaagact gggaaaagaa atgaaagttt tcagtgaaag acttcgctca
     6661 cagttagaca tgctgaagtc aattccgcat agtgcaaaat tcggaggggc tacaggaaac
     6721 ttcaatgctc atgcagtggc ttaccctgct gttgactgga tttcttttgc tgatgatttt
     6781 gtgaactcac tcggattaac acgcacaaca ttcacaacac agattgaacc ttatgacaac
     6841 ctttcggctt tattcgataa tctaaaacgc atcaataata ttttattgga tttatgtcgt
     6901 gatgtttgga gttacatcag catgaattat ttttcgcaaa ctgtcaaagc cggtgagatt
     6961 ggttcttctg caatgccaca taaagttaat cccatagact ttgaaaatgc agaaggtaat
     7021 cttggcattg ccaatgcact tttcgaacat ctgtcagcca aacttcctat ttcaagactt
     7081 caacgtgatt tgactgatag cactgtgttg agaaataccg gtgtgccgtt tgcacataca
     7141 ctcattgcac tgaaatcttt actgaaagga ttaaataaaa tttcgccaaa cactcaggta
     7201 atttctgctg acctaaatcg tcatgcagta gtaattgcag aaggcataca gacaattctc
     7261 agacgcgaag gctaccctaa tccttatgaa gcattaaaag atttaacacg aggaaaagag
     7321 aatatcactt tgcaggatat tcatcagttc atcaacacac ttcaggttgg tgatacagta
     7381 aaagctgaat tgctgaaaat tactcctgaa aattataccg gtgtaaattg gtcagactga
     7441 tttttccgtc agccttgcaa catattttcc gataatatca aactcaacat ttacggtttg
     7501 tccgcgttta agatttttaa atgtggtatg ctgcatggtg aatggaatta ttgcaactga
     7561 aaacatataa tcaccggcat caactactgt aaggcttaca ccgttgacag ttacagagcc
     7621 tttctcaact attaaatttc gtgctgagcc atcattttca aaaataaatt tccagcttcc
     7681 attttcatct gttatagaag tgcaaacagc agtaccgtca acatgcccct gtacaatatg
     7741 tccgtctaaa cgtgtattca acaacatgcc tctttcaaga tttacttcat tgccttcaac
     7801 caaactacta aggtttgttt tgcgcaatgt ttcatcaaca gcagtaacct tataggtttt
     7861 gttttcatga ttaatattta caacagttaa gcaaacacca ttgtgtgcaa cactctgatc
     7921 tatttttaat tcatcagcaa aagaacatga aacagtaaaa tgacgattac tgccttcatt
     7981 ttctattttc tttacaatgc ctaaagtttc aataattcct gtaaacatta tttagcacct
     8041 ccaatcaggt taacagc
//
LOCUS       DAOOOI010000084         7998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1779_length_7998_cov_21.165177, whole genome shotgun sequence.
ACCESSION   DAOOOI010000084 DAOOOI010000000
VERSION     DAOOOI010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1779_length_7998_cov_21.165177"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(283..688)
                     /gene="tnpA"
                     /locus_tag="PKX59_09135"
                     /pseudo
     CDS             complement(283..688)
                     /gene="tnpA"
                     /locus_tag="PKX59_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(730..1677)
                     /locus_tag="PKX59_09140"
     CDS             complement(730..1677)
                     /locus_tag="PKX59_09140"
                     /inference="COORDINATES: protein motif:HMM:NF017238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ91069.1"
                     /translation="MIDFVAPLTKNKLVLSDSKLVSDSGEKFPVIDGIPRFVELDNYA
                     NAFGLQWKSFAKIQLDSYNGSTISKDRLVRCLGTSLAELQGKTILEVGCGAGRFTEHL
                     VSSGALVHSVDLSAAVEANHDNISDKPNYRIAQADVYKLPFNENSFDIVICLGVIQHT
                     PSPEKTIHALWNMVKPGGTLVIDHYTFDWMYLLKPIMFYRFFLSRMKPEKSKRIVESL
                     VGFFFPLHWKYRSSKLMTMLLNRLSPCYVYIKTFPKMDYDFHFHLTRLDTYDGLTDYY
                     KHLRSKTQIENILKKFKPHSLSVWKGGNGVEARAIKPIG"
     gene            complement(1704..2906)
                     /locus_tag="PKX59_09145"
     CDS             complement(1704..2906)
                     /locus_tag="PKX59_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOZ91070.1"
                     /translation="MKINYIFLVFLLPFSVIAQQYVWTQKSSLPAALRYNPVGFSFQN
                     KGYIGLGVGTSGSLNDLWEYDDSSDSWTQKASYPGAGRYGAACFVIGEFAYVGCGWSP
                     NPVVDFYRYHVPSNAWYPIASYPGTPFYTGVNFSIGGKGYTGVGFSPYTHEFYEYDTL
                     TNIWTQKADFAGGIRQSTYSFSYNGKGYVTTGSHGWPANPYNDMWQYNPAADAWTQKS
                     SMPGAVRYGCSGFVIDSIAFVGLGQNGTSYFNDMYAYNINADTWTIADSFPGTPRVFP
                     GNFSIGNSGFLCAGGISGLNVNFNDCWEYGLVSGISNKEEHVILNSQPNPCTDFFILD
                     IPNTFSSAPRLTLIDARGKVVLSKVVQVGKNRINIDRKLLSGVYYGFCFDNRNQKATC
                     KIVVRKDS"
     gene            3089..5005
                     /gene="asnB"
                     /locus_tag="PKX59_09150"
     CDS             3089..5005
                     /gene="asnB"
                     /locus_tag="PKX59_09150"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019827659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOZ91071.1"
                     /translation="MNYICRPMCGITGFLDFNKKSALEMLRQMSATLHHRGPDDEGNE
                     IIQHPNVAIGMGFRRLAIIELSSAGHQPMKSADGNFTIIFNGEIYNHAELRKQLEQEG
                     LSFRSQSDTEVILQSFSRWGISCIQKFIGMFAIALYDSKEEKVYLIRDRAGVKPIYWY
                     FKNDLFLFGSELKAFHRHPSFQKEIDHGAISLYFQHGYIPAPHCIFKNTYKLLPGHYL
                     TIDLRSKEIRNSCYWDVSDFYNKPKLAISETEAITETEKILTKAFNYRMVADVPVGIF
                     LSGGYDSSLVTALLQKERTQKLKTFTIGFEQESYNEAKHARKVAEHLGTEHYEYTCTY
                     KEAMDIIPMLPEISDEPLADSSLIPTYLVSKMARKQVTVALSADGGDETFAGYTKYAK
                     ATDYLNKLQKLPSIAKTAAQIPALLWKMTSSNSLSIPDRADKLQLILRAKHPVEAFNI
                     ITQGMTQNEVSSLLMNHITFPLSAFDDFDKLKAPELLDNFLWLDYKTFLSDDILQKVD
                     RATMAVSLEGREPLLDHNIIEWAAQLPSAFKLNNGTSKYLLRKITHQYIPKEIMEREK
                     MGFQIPLHLWLKSEMKDLLLHHLSEESISKYQIFKQAEVNAILNAYLNNNNLDFYRLW
                     KLFNLQMWLDRWTT"
     gene            complement(5002..6414)
                     /locus_tag="PKX59_09155"
     CDS             complement(5002..6414)
                     /locus_tag="PKX59_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91072.1"
                     /translation="MKKLLLYINVIALMIIAIDVWGQNESNIWYFGNHAGIDFNSGSP
                     VAITNGGMMTYDNASAICDSAGNLLFYTDGQMVWNANHQIMSNGVNIGGSTTGGQAAV
                     IVKKPLSNSIYYIFTSDGFGASGGLEYSIVDMSLLGGLGEVTQLGTLLQIPSTEKLAG
                     IHTCTEKFTWLITHRGNSNEFCSYKITQSGLDTVPVVSAIGTFIPAGPYGVVNSTAGQ
                     MSLSKDGSKLASVNYQSGTVEIFDFNVASGHITNQILLTNTYLHAWGVEFSADASKLY
                     LSVYNSPSILQFDLNSGNATTITGSEISIGNISGTYCPGFSGATGYLQRGPDDKIYLA
                     KCGSTYISVINSPNDAGTLCGFVDNGIYLNSKVSTFGLSRSIAHECESTGVKNVDKNE
                     LFSVTPNPSNGSFSINFSENNFRGIRVLKIFNSMGEVVYFEKVDNSETKNVSTNLAAG
                     IYTLELNLDNQSVFKKIVVY"
     gene            6496..7965
                     /locus_tag="PKX59_09160"
     CDS             6496..7965
                     /locus_tag="PKX59_09160"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOZ91073.1"
                     /translation="MGVIQRQGLKNTITTYLGIAIGFVSLIVIQPQFLTKEELGLTRI
                     LYSFSILVAMFVPLGIGNATTKYFPLFKDSNKNHHGYFAFMMLFPLAGFVLAAAILFA
                     IKNFIIAQYISESPMFTEFYYYVFPLTFILSLITCLNVYCYANYKSTIPAFLNDVVAR
                     VLVIVVITVYFLKWITLNQFIACYVGLYGIQLLMLLAYIFIFDKPVFKIDWNYFKEKD
                     FKTLIRYGLLLWFAGVASIGLKYLDSIMLGKYLSLSFVGIYTIAAFIPTVIEAPLTAL
                     EKIAASRIAFAWAENNRKDILEIYRKSSLYMLALGGLLFTGININIENLLSFLPDGYQ
                     QGATVVFIISLGTLVNMLTGANAPILFNSDKYRWGAFFLIALALVSVALQMVFIPWLG
                     LNGAAIATVSCYVLYNLFMSVVVWKFYNLHPFDKKMLIILFAIIFCFAINYFLPAIEN
                     KILDMIHRSVIVTVVYLLLIYRYKVLEEFHHLLPWKRKK"
BASE COUNT         2563 a         1537 c         1301 g         2597 t
ORIGIN      
        1 ctgatgaatg caagaggaat aaaaatttta tttcgtggat taaaagaaaa agtaatcaca
       61 tcgtaacgct cttgcagcaa ttcaatatcc ctttgtacaa aggttgaata gttaggaaaa
      121 acgaaaatta gttttttctt tttcaaggaa aatatttgat tgcgaaagta aggattttgg
      181 aggtggggat cttcctcaca tcaggacttt agtcctgtaa ccccatgctg aagcatgggg
      241 ttactctata cgatgcctgg tacctcacac tttagtgtga ggtcaaagaa actcatcaca
      301 ttcctgagta aaaggaattt tcttgtgatg ctcctcctga ttgtcaatat actttcttac
      361 tgcatcaaga gcagattcgc taactgatgc tgcataatat tcattagccc attcgaattt
      421 tgattctgta agttgttctt tatttgccca aaaagccgat tcgcccttta tcaactgcat
      481 cgctttagca atactcatat cagcattcaa ccacatcaaa cagtgcaaat gatctgtatg
      541 gccattaatt ctgtcaataa aaatattttt caccttggca ttttctctga tatgttttat
      601 tagcttttta cgtgcttctt tagtgagaaa aggatgtctg ttttttggta ccccaaacaa
      661 catgcacaat aattttcaca aatgccattc tatatgtggt ttgatatgaa gataaaactt
      721 tgtggaatat cagcctatag gcttaattgc tcttgcctca acaccattcc cacccttcca
      781 aacacttaag gaatgtggct taaacttctt taaaatattt tcaatttgtg tcttacttcg
      841 cagatgctta taataatctg taagtccatc atacgtgtca agtcttgtca ggtgaaagtg
      901 aaaatcgtag tccatcttcg gaaatgtttt aatataaaca taacatggcg acaaacgatt
      961 aagcaacata gtcatcaact tactacttct gtatttccag tgtagcggaa agaaaaatcc
     1021 aacaagactt tcaactattc tttttgattt ttcaggtttc attcttgata gaaagaaacg
     1081 ataaaacatg ataggtttca ataaatacat ccaatcaaac gtatagtgat caataaccaa
     1141 tgtaccacca ggtttcacca tgttccacaa tgcatgaatt gttttttcgg gagatggtgt
     1201 atgttgaatc acaccaaggc aaataacaat atcaaagcta ttttcattaa aaggcagctt
     1261 gtaaacatca gcttgcgcaa ttctataatt gggtttatca ctaatgttat catgatttgc
     1321 ttctactgct gcactcaaat caacagaatg aaccaaggca ccactactca ccaaatgttc
     1381 tgtaaaacgt cctgcgccac aaccaacttc aagtatagtt ttaccttgca actctgctaa
     1441 tgatgtacct aaacatcgta ctaaacgatc tttagaaata gtgctgccat tataactgtc
     1501 aagttgaatt ttagcaaaag atttccattg taacccaaaa gcgttagcat aattatcaag
     1561 ctcaacaaaa cgcggaattc catctataac aggaaatttc tctccactat ctgatactaa
     1621 tttactatca gacaaaacaa gtttgttttt tgttagtggt gcaacaaaat caatcatgcg
     1681 cgcgaaagta ttgaaaagat tgtttagcta tcttttctaa caacaatttt acatgttgct
     1741 ttctgattcc tgttatcaaa acaaaaacca taataaacac ctgataataa tttcctatca
     1801 atatttattc tgttttttcc aacttgaaca actttactca ataccacctt acctcttgca
     1861 tcaatcaaag ttagtctagg tgcagaagaa aatgtatttg gaatatcaag aataaaaaaa
     1921 tcagtacatg gattaggttg tgagtttaat attacatgct cttccttatt tgatattcct
     1981 gaaaccaaac catattccca acagtcatta aaatttacat taagtcctga aattccaccg
     2041 gcacataaaa aaccggaatt tccgattgaa aaatttccgg gaaatactct tggagttcct
     2101 ggaaacgaat ccgcaatagt ccatgtatct gcattaatat tatatgcata catatcgtta
     2161 aaataacttg ttccattctg tccaagtcct acgaaagcta tcgaatcaat aacaaaaccg
     2221 gaacaaccat atcgaacagc accgggcatt gacgattttt gtgtccaagc atcagccgct
     2281 ggattatatt gccacatatc gttgtaagga ttagcaggcc atccatgaga acctgtggtt
     2341 acatatcctt taccattgta tgaaaaacta tatgtacttt gtctgatgcc tcctgcaaaa
     2401 tcagctttct gcgtccatat atttgtaagt gtatcatatt cgtaaaactc atgtgtatat
     2461 ggcgaaaatc caacacccgt atatcctttt ccgccaatag aaaaatttac tcctgtataa
     2521 aatggtgttc cgggatatga tgctatagga taccatgcat tagaaggaac atgataacga
     2581 taaaagtcaa ctacaggatt aggcgaccag ccacaaccta cataagcaaa ttctccgata
     2641 acaaaacatg cagcgccata acgacctgca cccggataag atgccttttg tgtccatgaa
     2701 tcggaagaat catcatactc ccataagtca ttcaatgagc ccgatgtacc tactcccaat
     2761 ccgatatatc ctttgttttg aaaagaaaat cctacaggat tataacgtaa agcagcaggt
     2821 aagctcgatt tttgtgtcca aacatattgt tgtgcaatta ctgaaaatgg caatagaaaa
     2881 actaaaaaaa tgtaattgat tttcattgtc taattttatt gagctaatat actaactaaa
     2941 aatcataatt actgtgtttt ctgtgggctg aagtccggca gtaccttata tgcatcctta
     3001 gcccccatgc tgaagcatgg atttactcac ttcgattcca tttttatatc tcttcgtact
     3061 tcattgcaga tatagacttt accccacgat gaattatatt tgccgcccta tgtgtggtat
     3121 taccggattt ttagatttta ataaaaaaag cgccttagaa atgttgcgac agatgtctgc
     3181 tacgctacat caccgtgggc ctgatgatga aggtaatgaa ataattcaac atccaaacgt
     3241 tgcaataggg atgggatttc gcagacttgc cataattgag ctctcatcag caggacatca
     3301 acccatgaaa tcggctgatg gcaactttac aattattttc aatggtgaaa tttataacca
     3361 tgcagaacta cgcaaacaat tagagcaaga agggctttca ttccgttcgc aaagcgatac
     3421 agaagttata cttcagtctt tctcacgatg gggaattagt tgcattcaga aatttattgg
     3481 aatgttcgcc attgcattat atgattcaaa agaagaaaaa gtttatctga taagagatcg
     3541 tgccggtgta aaacctatat attggtattt taagaatgac ttatttcttt ttggttctga
     3601 attaaaagca tttcaccgcc atccttcatt tcaaaaagaa atagatcacg gtgccattag
     3661 tctttatttt caacatggat acattcctgc tccacattgt atttttaaaa acacatacaa
     3721 gcttttaccc ggtcattact taacaattga tttaagaagt aaagaaatcc gtaactcatg
     3781 ttattgggat gtatccgatt tttataacaa acctaaactt gctatttctg aaactgaggc
     3841 aataactgaa acagaaaaaa tattaaccaa ggcttttaac taccgtatgg ttgctgatgt
     3901 tcctgtaggc atatttttaa gtggtggata tgacagctca ttggtgacag cattattgca
     3961 gaaagaaaga actcaaaagc taaagacatt tactatcggt tttgaacaag aaagttataa
     4021 tgaagccaaa catgcacgca aggtagctga acatttagga acggagcact atgagtacac
     4081 ttgcacctat aaagaagcaa tggatattat ccctatgctc cctgaaatat ccgatgaacc
     4141 attagctgat tcttcattga tacctacgta tttagtcagt aagatggcac gcaaacaagt
     4201 tactgttgca ttgagtgccg atggcggtga cgaaactttt gcaggctaca ccaaatatgc
     4261 aaaggcaacc gattatctta ataaacttca aaagctaccg agcattgcaa aaacagctgc
     4321 acagattccg gctttactct ggaaaatgac ttcatcaaac agcctttcca tcccggacag
     4381 agcagacaaa ctgcaactga tactaagagc aaaacatcct gttgaagcat ttaatattat
     4441 cactcagggt atgacacaaa atgaagttag cagcctttta atgaaccata ttacttttcc
     4501 actttctgca tttgatgatt ttgataagtt aaaggcacct gaactacttg acaatttttt
     4561 atggcttgat tataaaactt ttctttctga cgatatttta cagaaagttg accgcgcaac
     4621 gatggctgta agtcttgaag gcagagaacc tctgctcgac cacaacatta ttgaatgggc
     4681 tgctcaatta ccctctgcgt ttaaactcaa caatggaacc tcaaaatatc tgttgcgcaa
     4741 aataacacat caatatattc caaaagaaat aatggaaagg gagaagatgg ggttccaaat
     4801 accactacat ctttggctaa agagcgagat gaaagattta ttattacatc atctttctga
     4861 agaatctatt tctaaatatc agatatttaa acaggcagaa gttaatgcaa tactcaatgc
     4921 atatctaaat aacaacaatc ttgattttta tcgcttgtgg aaattattta atcttcaaat
     4981 gtggcttgat aggtggacaa cttaataaac aacaatcttt ttaaaaactg attgattatc
     5041 taaatttaat tctaaagtat aaatcccggc tgcaagattt gtactaacat tctttgtttc
     5101 agaattatct actttttcaa aataaactac ttctcccatt gaattaaaaa ttttcaatac
     5161 tcgaatccct ctaaaattgt tttctgaaaa attaatacta aaacttccat tacttgggtt
     5221 gggtgtgact gaaaacaatt cgtttttatc aacattcttt acgccagtac tttcacattc
     5281 atgtgcaatt gatcttgata agccaaaggt cgagacttta gaattaagat aaatcccatt
     5341 atctacaaaa ccacaaagtg ttcccgcatc atttggggag ttgattacac taatataagt
     5401 tgaaccacac ttagctaaat aaattttatc atcaggtcca cgttgaagat accctgtggc
     5461 tccagaaaat ccgggacaat aagttccgga tatatttcca atgctaattt cagaaccagt
     5521 tattgtagtt gcatttcctg aatttaaatc aaactgtaaa attgaaggag agttataaac
     5581 tgataaatat aatttggatg catcagccga aaattcaact ccccaagcat gaagatatgt
     5641 attagttagt aggatttgat tggttatatg gccagatgcc acattgaaat caaaaatctc
     5701 tacagttcca ctttgataat ttacacttgc taatttactt ccatctttag aaaggctcat
     5761 ctgccctgct gtactattca caacaccata gggccctgcc ggaataaatg ttccaatagc
     5821 actaaccacg ggtactgtat ctaatccgct ttgagtaatt ttataactac aaaattcgtt
     5881 tgaatttcct ctatgggtta ttaaccaagt aaacttttca gtacaagtat gaataccggc
     5941 aagtttttca gttgagggaa tctgaagtaa tgtacctaat tgagtaactt cacctaatcc
     6001 ccctaataaa ctcatatcta caattgaata ctccaaacca cctgaagcac caaaaccatc
     6061 tgaagtaaaa atataataaa ttgaattgct taatggcttc ttaactataa ctgctgcctg
     6121 tcctcctgtt gtactaccgc ctatattaac gccattactc attatctgat gattcgcatt
     6181 ccaaaccatt tgaccatctg tataaaacaa taaattccct gctgaatcac aaatagcact
     6241 tgcgttatcg tacgtcatca ttcctccatt tgtaattgca acaggactac ctgagttaaa
     6301 atctattcct gcatggtttc caaagtacca aatgttggat tcgttttgcc cccaaacatc
     6361 tattgctata atcatcaacg ctatgacgtt gatataaagt aataatttct tcatgactta
     6421 atttatttat tagttactct tgctaacaaa attacactta ttttttaaat aaacaagcag
     6481 gaataattat ttttcatggg cgtaattcaa cggcaaggat taaaaaatac cattaccact
     6541 tatctgggta ttgccattgg ctttgtgagt cttattgtaa ttcagccgca gtttcttaca
     6601 aaggaagagc ttggtcttac ccgtattctg tactccttct ccattttagt tgccatgttt
     6661 gtgcctcttg gcattggcaa tgccactacc aaatattttc cgctgtttaa agacagcaat
     6721 aaaaatcatc atggctactt tgcctttatg atgctgtttc cattagccgg atttgttttg
     6781 gcagcagcaa ttctttttgc aataaaaaat ttcatcatag cacaatacat tagcgagtcg
     6841 ccaatgttta ctgagtttta ttattatgtt tttccgctta catttattct ttcactcatc
     6901 acctgcctga atgtttactg ttatgccaat tacaagtcaa ctattcctgc ttttttaaat
     6961 gatgtagttg caagagtgct ggtgattgtt gttatcactg tttattttct gaaatggatt
     7021 acgctaaatc agtttattgc ctgttatgtg gggctttatg gtattcaact gttgatgtta
     7081 ttagcctata tttttatttt tgataagccc gtttttaaaa ttgactggaa ttattttaaa
     7141 gaaaaagatt ttaaaacact catacgctac gggttgctgc tgtggtttgc aggagtagct
     7201 tcaataggat taaaatattt agacagcatt atgcttggaa aatatttatc gctgagtttt
     7261 gtgggtattt acaccattgc agcatttatc cccacagtga ttgaagcacc attgacagca
     7321 cttgaaaaaa ttgccgcctc gcgcattgca tttgcatggg cagaaaataa cagaaaagat
     7381 attcttgaaa tttaccgcaa gtcgtcattg tatatgctgg ctttgggtgg attgttgttt
     7441 actggtatca acataaacat tgagaattta ctaagctttt tgcctgatgg ctatcagcag
     7501 ggtgccactg tagtctttat tatcagcctt ggaactttag taaatatgct caccggtgcc
     7561 aatgcaccta tactttttaa ttctgataaa tatcgttggg gtgcattttt tctgattgct
     7621 ttagcccttg tttctgttgc tttgcaaatg gtttttatcc catggcttgg gctcaacggt
     7681 gcagcaattg ccacagtaag ctgttatgta ttatacaacc tgtttatgtc agtagtagtt
     7741 tggaaatttt ataatctgca tccattcgat aaaaaaatgt tgattatttt atttgcaata
     7801 atattttgtt ttgcaatcaa ttattttctg ccagcaattg aaaacaaaat tcttgatatg
     7861 attcatcgtt ctgtaattgt aacagtggtt tatctgctac taatctatcg ttataaggtg
     7921 cttgaagagt ttcatcacct gttgccctgg aaaagaaaaa aataatcaga cgttaaagcg
     7981 gaagtgcatt acatcgcc
//
LOCUS       DAOOOI010000085         7947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1794_length_7947_cov_33.121389, whole genome shotgun sequence.
ACCESSION   DAOOOI010000085 DAOOOI010000000
VERSION     DAOOOI010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1794_length_7947_cov_33.121389"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..627)
                     /locus_tag="PKX59_09165"
     CDS             complement(118..627)
                     /locus_tag="PKX59_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91074.1"
                     /translation="MKTLLCFLLLPTILVAQTFQKNQSEGNEDFIKRIAPHSANEVKY
                     LLSDSFNTSTEKIVYAYSIFEHGNLITNNDSTLTYFLCILVPTSVGALDYNMQQFRMV
                     ASYKKKARIESAEIIKSGREKKLQVYIAEMVRGPGGVPRDVKRTFVYKQEKESRMFTD
                     TFREVLGDY"
     gene            complement(687..1199)
                     /locus_tag="PKX59_09170"
     CDS             complement(687..1199)
                     /locus_tag="PKX59_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010603222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOZ91075.1"
                     /translation="MLIFLIGFMGAGKSSQAKKLAARLNFESIDLDELIEKTVKQSIS
                     EIFKISGEQHFRKIESETLKTLSEKQNAVIATGGGTPCFFDNMQWMNEKGITVYLKIH
                     PGTVYHRIAPSKSKRPLISHMNDADLMEYIMETMIERAPFYNQAKIQISGLNLKTEDL
                     VSALKPFLKD"
     gene            complement(1205..1765)
                     /gene="def"
                     /locus_tag="PKX59_09175"
     CDS             complement(1205..1765)
                     /gene="def"
                     /locus_tag="PKX59_09175"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOZ91076.1"
                     /translation="MILPIIAYGDPVLKKVGSSIDKDYKGLTQLIENMFETMYAASGV
                     GLAAPQVGISIRLFVVDTKPFAEDEPELENFKKVFINPQIIEEVGDEWKFSEGCLSIP
                     GIREDVMRKKIIHLRYQDEEFKTHETVFEGLAARVLQHEYDHVDGKLFVEKLSPFKKQ
                     LIKGKLRDITIGKVDVDYKMRFPIKK"
     gene            complement(1762..2190)
                     /gene="ruvX"
                     /locus_tag="PKX59_09180"
     CDS             complement(1762..2190)
                     /gene="ruvX"
                     /locus_tag="PKX59_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOZ91077.1"
                     /translation="MPRILAVDYGNKRVGLAVTDPMQIIATALTTVHSSKAVEFICNY
                     FNSEEVEEIVVGKPVQMDNTPSEIEKNILLFIKKLIDKCPSAKVIRYDERFTSVMAQR
                     TLLEAGLKKQQRQNKALIDKTSAVILLQSYLEHKRNFLKI"
     gene            2276..3091
                     /locus_tag="PKX59_09185"
     CDS             2276..3091
                     /locus_tag="PKX59_09185"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOZ91078.1"
                     /translation="MPVREKTVNAIWENRALLTDESNKKVIRSVIEDLDKGNIRVAEQ
                     MNNSWRVNEWIKKAVILYFPIQKMEVHHAGPMEFYDKIDLKTGYENLGVRVVPHAIAR
                     YGSFVAKGVIMMPSFVNIGAYVDSGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEP
                     VQASPVIIEDNCFIGSRCIVVEGVRIEQEAVLGANVVLTMSTKIIDVSGNTPVEYRGV
                     VPARSVVIPGSIPKTFAAGTYQVPCALIIGKRKQSTDNKTSLNDALREYDVAV"
     gene            3103..4059
                     /locus_tag="PKX59_09190"
     CDS             3103..4059
                     /locus_tag="PKX59_09190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOZ91079.1"
                     /translation="MHKILIVQTAFIGDVILATGLIESLHQAFPESKIDFVLRKGNES
                     LLKSHPFVNKLYVWDKKKNKFSNLINLISQIRKEKYDLVVNLQRFFSTGLITLFAKSK
                     NKRGYNKNPLSFCYHKKSEHIIGKKHETYRNFSLIDDLKNIQYQKPNLYFTEEENQKV
                     QSLKSKPFVCMAPASVWFTKQLPVAKWAELTLTIPENIKIYFLGAPADVALCNEIRLL
                     SSHPNIEILCGKLTLTESAALMQHAEMNYVNDSAPLHLASAVNAPVTAFFCSTVPEFG
                     FGPLSDKSIIKQVGEMSCRPCGLHGYKSCPLGHFDCAYKIKI"
     gene            4070..5452
                     /locus_tag="PKX59_09195"
     CDS             4070..5452
                     /locus_tag="PKX59_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOZ91080.1"
                     /translation="MFAVVDIETTGGSLTQGSRITEVAVVIHNGNTIEHRFSTLINPE
                     TDIPSFIIGLTGITNELVENAPCFKEIAEQLFQLLDGKIFVAHNASFDFSFIKSEFKR
                     CGIDFNAEVICTCSLSRKIFPGFGSYSLGNLTKNLGIILEGHHRAMNDAVAASEILEK
                     LVSEKGLEHTLLHRSVPIKNTEIPHELISKLPETPGIYFFTDSNHEILYIGKAKNIKK
                     RVTAHFRNNNSKSKKIKALLHDISYMNTGCELLACLLEIEYIKEYQPFFNRAGKSEKN
                     FGVYKNDILNGLSKFFIHDISKTNSSPLRYFGSRKAADEFLSHITEKYSLCRCINSLE
                     SYSDNCFYKQIKQCNGIELDNNEKNNYNNRFNDACLEVGDGWHDAALAYPIAGSEEWA
                     YVFRHQGAIGYYFGKKATTFNINLIKGVTHLKNHLESQSIFNSFLKKPSAKKIKIISG
                     EIREVEIEKK"
     gene            5462..5869
                     /locus_tag="PKX59_09200"
     CDS             5462..5869
                     /locus_tag="PKX59_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HOZ91081.1"
                     /translation="MDNRHDIITPDDITLMVDTFYSAIREDDLLGPIFNNIIQDRWPE
                     HLKKMYGFWRSILFNEPLYNGSAFEAHRQLPIEMKHFKHWLKLFEATVDGLFEGSNAE
                     TAKSRARKIAEVFYYKIESERGNEPQNFSPTAG"
     gene            complement(5856..6335)
                     /gene="ispF"
                     /locus_tag="PKX59_09205"
     CDS             complement(5856..6335)
                     /gene="ispF"
                     /locus_tag="PKX59_09205"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOZ91082.1"
                     /translation="MNFRVGFGFDVHQLEQGIPFFLGGVRIEHYKGAKGHSDADVIIH
                     AICDALLGAAALDDIGKHFPDTSNEFKGIDSSILLAKVVSLLKEKSIAIGNVDVSVVL
                     EKPKIAPYIQAMREKLASVMQIEIDCVSIKATTNEKMGFVGREEGVAAYAVALINRQ"
     gene            complement(6339..7541)
                     /gene="porV"
                     /locus_tag="PKX59_09210"
     CDS             complement(6339..7541)
                     /gene="porV"
                     /locus_tag="PKX59_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010603321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOZ91083.1"
                     /translation="MRYKLFYGALSLAVLSVQNQVSAQTDKANLLGQINTITTAVPFL
                     MISPDARAGGMGDAGVASSADVNSIHWNPAKLVFADKKMGVGISYTPWLRKLVPDINL
                     AYVSFFTKPNDVSAIGASLRYFSLGNIDFTSETGDPMGSYKPNEFAVDVAYSRKLGDE
                     FSGGIALRYIHSSLTGGITVGGNATKAGNSVAADVSFFYKTNLEISGKDAEFGAGLAI
                     TNIGSKMSYTDKGTKDFIPINLRLGPSLKIKLNDYNELAFLVDFNKLLVPTPPVYSDS
                     TIAGEENPIIFAGKDPNVGVAQGIFQSFGDAPGGGKEELREINIAGGLEYWYDKQFAF
                     RAGYFYENKTKGNRKYFTLGIGLRYNIFGLDFAYLIPTEQRNPLENTLRFSLSFNFDS
                     IKGSDSNE"
     gene            complement(7668..>7947)
                     /locus_tag="PKX59_09215"
     CDS             complement(7668..>7947)
                     /locus_tag="PKX59_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91084.1"
                     /translation="PNPFTTHTQFSFEYNKPCEDLEVQVQIFTIAGKLVKTISKKIIT
                     PGTRIDDLTWDGRDDFGDRIGRGVYIYRVKLNTADESASETQKLVILK"
BASE COUNT         2625 a         1477 c         1380 g         2465 t
ORIGIN      
        1 ctttagcaat aaattgttaa gcgatatgaa aataagggtg aatgtagcca ttcacaacta
       61 tgaaaatctg aaaaaaagaa cgtcagcaat aaatcagcat attaaggaca actgttttta
      121 atagtcaccc aaaacctctc tgaaagtatc agtaaacatt ctgctttctt tttcctgctt
      181 ataaacaaaa gtgcgcttta catctcttgg cacacctccg gggcctcgta ccatttctgc
      241 aatatatacc tgaagtttct tttcccgtcc cgacttaata atctctgcac tctcaatcct
      301 tgctttcttt ttatagcttg ccaccatacg aaactgctgc atgttgtaat ccaatgctcc
      361 tacagaagta ggaacaagga tgcataaaaa ataggtcaaa gtactatcgt tgtttgttat
      421 caggttgcca tgctcaaaaa tgctgtaggc ataaacaatt ttttctgttg aagtattaaa
      481 agaatcactc agaagatatt taacttcatt agccgaatgt ggagcaatcc gtttgataaa
      541 atcctcattg ccttcacttt gatttttttg aaatgtttgt gcaaccaata tggttggaag
      601 cagcaaaaag cataataatg ttttcataat attttatttt gaaaggtaaa cataaaaaaa
      661 aatagtcaat ctgaaatgct attgttttaa tctttcaaaa atggcttaag tgcacttacc
      721 aaatcttctg ttttaagatt tagtcccgaa atctgaattt ttgcctgatt atagaaaggt
      781 gccctttcaa tcatcgtctc cataatgtac tccatcaaat ctgcatcgtt catgtggcta
      841 atcaaaggtc gtttagattt tgaaggtgca atgcggtgat aaactgtgcc gggatgtatt
      901 ttcagataaa cagtaatgcc tttttcattc atccattgca tattatcaaa aaagcatggt
      961 gtgccaccgc ccgttgcaat cactgcattt tgtttttcag ataatgtctt taaagtttca
     1021 ctctcaattt tacgaaaatg ttgctctccg cttatcttaa aaatttctga aattgattgc
     1081 ttaactgttt tttcaatgag ttcatccaga tcaatgcttt caaaatttaa tcttgcagct
     1141 aatttctttg cctgcgatga ttttccggct cccatgaatc caatcagaaa tatcaacatt
     1201 gctgctattt ctttattggg aaacgcattt tgtaatcaac atcaaccttg cctatggtaa
     1261 tgtcacgaag tttgcctttt atcaattgct ttttaaaagg tgataatttc tcaacaaata
     1321 atttaccatc aacatgatcg tactcatgtt ggagtacccg tgctgccaat ccttcaaaaa
     1381 ctgtttcatg tgttttaaat tcttcatcct gatagcgcag atgaattatt tttttacgca
     1441 tcacatcctc tcttattccg ggaatgctca aacaaccttc gctaaacttc cattcatcac
     1501 ctacctcttc aattatttgt ggattaatga atactttctt gaaattttcc agctccggtt
     1561 catcttctgc aaatggtttg gtgtcaacaa caaatagtct gatggaaata ccaacctgcg
     1621 gagcagccaa cccaacaccg gatgctgcat acatagtctc aaacatgttt tctataagtt
     1681 gtgtaagacc tttataatct ttatcaattg atgagcccac ctttttcaaa accgggtcgc
     1741 cataggcaat aattggtaat atcatatttt caaaaagttt cttttatgtt ctaagtacga
     1801 ttgtaataat ataacagcgc ttgtcttatc aatcaatgct ttattttgtc tttgttgctt
     1861 tttcagtccg gcttccaaca atgttcgttg tgccattaca gaggtaaaac gttcatcata
     1921 tcttatcact ttcgcactcg gacatttatc aataagcttt ttgatgaaca acaaaatatt
     1981 tttctcaatt tcagatggtg tattatccat ttgtacaggt ttaccaacaa caatttcttc
     2041 tacttcttca gaattaaaat aattacaaat aaattcaacc gctttagagg aatgaacagt
     2101 agttaaagca gtggcaataa tctgcattgg atcagtaact gcaagaccta ctcttttatt
     2161 tccgtaatca actgcaagta tgcgcggcat atctgaattt aaagatgcaa aaataaaaaa
     2221 caacgggcaa tgaatgcatt aaggtggtta tatttgtagc tgaaaaataa tatttatgcc
     2281 tgtaagagaa aaaacagtca atgctatttg ggaaaacaga gcgttactta ccgatgaatc
     2341 aaacaagaaa gttatccgct cagttatcga agacctagat aaagggaaca ttcgggttgc
     2401 agagcaaatg aataatagtt ggcgcgtaaa tgagtggata aaaaaagctg tcatcttata
     2461 ttttccgatt caaaaaatgg aagtacatca tgcaggaccg atggagtttt atgataaaat
     2521 agatcttaaa acaggttatg aaaacttagg tgttcgtgtt gtgccacatg ccattgctcg
     2581 ctatggaagc tttgttgcaa aaggtgtcat catgatgcca tcgtttgtga atattggtgc
     2641 ttatgttgac agcggaacaa tggttgacac ctgggctact gttggcagtt gtgcacagat
     2701 aggaaagaac gtacacctta gtggaggagt tggcattgga ggagttttag agccagttca
     2761 ggcatcaccg gtaattattg aagacaattg ttttataggg tcgagatgta tagttgttga
     2821 aggcgttcgt attgaacagg aagcggtttt gggggctaat gttgtattga ctatgtcaac
     2881 aaaaataatt gatgtgtcag gaaatacacc tgttgaatat cgtggtgttg tgcctgcacg
     2941 ttctgtggtc attcccggtt caattcctaa aacttttgca gcaggaacat atcaggtgcc
     3001 ttgtgcactt attataggta aaagaaagca atctaccgac aataaaacat cactcaacga
     3061 tgcattgcgc gaatatgatg ttgcagtctg agactaacaa gtatgcataa aatattaatt
     3121 gtacagacag catttattgg cgatgtaatt ttagctacag gattaattga atctctgcat
     3181 caggcttttc cagaatcaaa aattgatttt gttttaagaa aaggtaatga gtcgttattg
     3241 aaaagccacc cttttgtaaa taaattatat gtctgggata aaaagaaaaa caaattttct
     3301 aatctcatta acctcatcag tcaaatcagg aaagaaaaat atgatttggt tgttaacctg
     3361 caacgatttt tcagcacagg tctgataact ttattcgcta aatcaaaaaa caaaagaggc
     3421 tacaataaaa atccattgtc cttttgttac cacaaaaaat cagagcatat cattggtaaa
     3481 aagcatgaga cttatcgtaa cttttcattg attgatgact tgaaaaatat tcaatatcaa
     3541 aaaccaaatt tgtatttcac agaggaggaa aaccaaaaag tgcagtcatt aaaatcaaaa
     3601 ccatttgtct gcatggcacc ggcttctgtt tggtttacca aacaattacc tgtagccaag
     3661 tgggcagagt taactctgac tattcctgaa aatataaaaa tatattttct tggcgcaccg
     3721 gctgatgttg cactttgcaa cgaaataagg ttgctttctt cacatccgaa tattgaaata
     3781 ttatgcggca aactaacact tacagagtcg gcagcactta tgcaacatgc tgaaatgaac
     3841 tatgtaaacg actctgctcc actccatctt gcatcggctg ttaatgctcc tgtgactgca
     3901 tttttctgct ctacagttcc tgagtttggc tttggtcctt tgtcagacaa aagcatcatt
     3961 aaacaagtcg gtgaaatgag ctgccgaccc tgtggattgc atggctataa atcttgcccc
     4021 ttaggtcatt ttgattgcgc atataagatt aaaatttaag taacgtggca tgttcgcagt
     4081 tgttgacata gaaactacag gaggaagttt aacacaaggt agccgaatta ccgaagttgc
     4141 agtagtgatt cataatggca atactattga acatcgtttt tctacactga taaatcctga
     4201 aacagacatt ccatcattta ttatagggct gacaggcatc actaatgaat tagttgaaaa
     4261 tgcaccatgc tttaaagaaa ttgcagagca actgtttcag ttgcttgatg gaaaaatttt
     4321 tgtagcacat aatgcgtctt tcgatttcag ttttataaaa tcagaattta aacgctgcgg
     4381 aattgatttt aatgcagaag taatatgcac ttgttctttg agcagaaaaa tcttccccgg
     4441 ttttggctcc tactctttag gaaatctcac aaaaaacctt ggaataattt tagaaggtca
     4501 tcatcgtgcc atgaacgatg ctgttgctgc cagtgaaatt ttagagaaat tggtttcgga
     4561 aaaaggtctt gaacacacac tgcttcatag gagtgtacca attaaaaata cagaaatacc
     4621 acatgaactt attagcaagt tgcctgaaac acccggcata tatttcttta cagacagtaa
     4681 tcatgaaatt ctgtatatcg gcaaagcaaa aaatatcaaa aaaagagtta cagcacattt
     4741 tcggaacaac aattccaaat cgaaaaaaat taaagctctc ttgcatgata taagctacat
     4801 gaatacaggg tgtgaattac tggcatgttt actcgaaatt gaatacatta aagaatatca
     4861 gcctttcttt aatcgtgccg gcaaatcaga aaagaatttc ggggtttata aaaatgatat
     4921 tttaaatggc ttatcaaaat tctttattca tgatatttca aaaacaaact catcaccatt
     4981 aagatatttc ggttcaagaa aagctgccga tgaatttctg tcgcacataa cagaaaaata
     5041 tagtctctgt cgttgcatca atagtcttga gagctacagt gacaactgtt tttataaaca
     5101 aatcaaacaa tgcaatggaa tagaactcga taacaacgaa aaaaacaact acaataaccg
     5161 ttttaatgat gcctgtttag aggtaggcga tggctggcat gatgctgcac ttgcctatcc
     5221 gatagctggt agcgaagaat gggcttacgt attccgacat cagggtgcta taggatatta
     5281 ttttggaaag aaagcaacaa cattcaatat taatctaatt aaaggagtaa ctcatttaaa
     5341 aaatcattta gaatcacaaa gtatcttcaa ttcattctta aaaaaaccat cagccaaaaa
     5401 aataaaaata atatcgggtg aaatcagaga agttgaaata gaaaaaaaat aaaaaatgag
     5461 gatggataat agacatgaca taattacacc tgatgatata acattaatgg ttgatacatt
     5521 ttacagtgct attcgtgaag atgatttgtt aggtccaata ttcaacaaca tcattcaaga
     5581 tcgctggcca gaacatctga aaaaaatgta tggtttctgg cgtagcattc tcttcaacga
     5641 accgttatac aatggaagtg catttgaagc ccacaggcaa ttgccaatag aaatgaagca
     5701 ttttaaacac tggcttaaac tatttgaagc tacagtagat ggtttgtttg aaggcagcaa
     5761 tgcagaaacc gcaaaatcaa gagcaagaaa aattgcagaa gtcttttact ataaaattga
     5821 atcagaacgt ggcaatgagc cacagaattt tagtcctact gccggttaat caaggcaact
     5881 gcataagcag caacaccttc ttcacgacca acaaaaccca ttttttcgtt ggtagttgcc
     5941 ttaattgaaa cacagtctat ttcaatctgc attacagaag ctaatttctc ccgcattgcc
     6001 tgaatatacg gtgcaatttt aggtttctcc aaaactacac tcacatcaac attaccaata
     6061 gcaattgatt tttcctttaa taatgaaaca acttttgcta ataaaattga ggaatctatt
     6121 cctttaaatt cgttggatgt atccggaaaa tgtttaccaa tatcatcaag tgctgctgct
     6181 ccaagcaagg catcgcaaat ggcatgtata attacatcgg catcactatg tcctttggca
     6241 cctttataat gttcaattcg aactccgcct aaaaaaaaag gaatgccctg ttctaactga
     6301 tgaacatcaa aaccaaaacc aactctgaaa ttcaccgttt attcgttact gtcgcttcct
     6361 ttgattgaat caaagttgaa tgacaagcta aaacgtaaag tattttctaa tggattgcgc
     6421 tgctctgttg gaataagata agcaaaatca agtccgaaaa tgttgtagcg taaacctata
     6481 cctaaagtaa aatactttct gtttccttta gttttgttct cgtagaaata tcctgcacgg
     6541 aatgcaaatt gcttatcgta ccaatattcc aatcctcctg caatattaat ttcgcgcaat
     6601 tcttcttttc ctcctcctgg cgcatcaccg aatgattgaa aaattccttg tgctacacca
     6661 acattaggat ctttccctgc gaaaataata ggattttctt cacctgcaat agtagaatcg
     6721 gaataaaccg gtggagttgg cactaatagc ttattgaaat caactaaaaa tgctaattca
     6781 ttatagtcat tgagttttat tttcaaagaa ggtcctaagc gcagattaat aggaataaaa
     6841 tcctttgttc ctttatcggt atatgacatt tttgaaccaa tatttgtaat ggccaaacct
     6901 gcgccaaact ctgcatcttt tcctgaaatt tcaagatttg ttttatagaa gaaagaaaca
     6961 tcggcagcta cagaatttcc tgctttagta gcattgccac caacagtaat tccacctgtt
     7021 aaacttgagt ggatataacg caaagcaata cctccgctaa actcatcacc caactttctt
     7081 gagtatgcaa cgtcaacagc aaactcattg ggcttgtacg atcccatagg gtcgcctgtt
     7141 tcagaagtaa aatcaatatt acctaaagag aaataacgta acgaagctcc aattgcagaa
     7201 acatcattag gcttggtaaa aaagcttacg taagccagat ttatatccgg cactaatttt
     7261 cgtaaccaag gtgtgtaaga aattccaaca cccatctttt tgtctgcaaa caccagtttg
     7321 gcaggattcc aatgaatact gttaacgtct gcagatgatg caacaccggc atcgcccata
     7381 ccaccggcac gggcatcggg agaaatcatc agaaacggaa cagcagttgt aatagtgtta
     7441 atctgaccaa gtaagttggc tttatcagtc tgtgcagaca cttgattttg tactgaaagt
     7501 actgctaaag acaaagcacc gtaaaacaat ttatatctca tgtataaaga aattcagtaa
     7561 taagttttaa aagctaagtt tgaataacga caataagtgg tttttatttt atgatgttat
     7621 ttttaagaag cggctggcaa atatacgata aaaaataaaa aaggtttcta tttgagaatc
     7681 accagttttt gcgtttctga agccgactca tctgctgtat tgagttttac tctgtaaata
     7741 tatactccac gaccaattct gtcgccaaaa tcatcacgac catcccatgt taaatcatca
     7801 attcttgtac cgggggtaat tatcttttta gaaatggttt taaccaattt accggcaatg
     7861 gtaaaaatct gaacctgaac ttctaaatct tcacaaggct tgttgtattc aaatgaaaac
     7921 tgcgtatgcg ttgtaaacgg gttggga
//
LOCUS       DAOOOI010000086         7763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1850_length_7763_cov_15.103269, whole genome shotgun sequence.
ACCESSION   DAOOOI010000086 DAOOOI010000000
VERSION     DAOOOI010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7763
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1850_length_7763_cov_15.103269"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1342
                     /locus_tag="PKX59_09220"
     CDS             128..1342
                     /locus_tag="PKX59_09220"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOZ91085.1"
                     /translation="MLTVDDFNFNGKRALVRVDFNVPLDKATFAVTDNTRIKAAVPTI
                     KKILTDGGSVVLMSHLGRPLKKLKDDGTVDTKKFTLKHTVSEVSKLMGVDVKFASDCI
                     GAETSSLASKLQPGEVLLLENLRFHKGEEKGDEEFARQLAALGNVYVNDAFGTAHRAH
                     ASTAIIAQFFPGAKMFGYVMANEIKNAERIMRNAEKPFTAIMGGAKVSDKILIIEQLM
                     SRCNHILIGGGMAYTFFKAMGGNIGTSLCESDKLDLAKQLLERAKQSGVQFHLPFDSV
                     LGDKFDNDAQTSISDNMNIPDGWMGLDIGPKAITAFSEIISQSKTILWNGPMGVFEME
                     KFAAGTKGIADAIVNATSKGAFSLIGGGDSAAAINKFGLADKVSYVSTGGGALLEYFE
                     GKELPGIKMIQS"
     gene            1857..>7763
                     /locus_tag="PKX59_09225"
     CDS             1857..>7763
                     /locus_tag="PKX59_09225"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HOZ91086.1"
                     /translation="MLKTQHKKERSVSCWRWIMCFMFLIGMWTTGAHAQTITIGTGTT
                     TTTGNGTPIYRSSSTSSFHHSKSIQLLTAAQLSGAGLTAGANITGYGYNKQNNGQPAG
                     ANGWTINVYLKNSSATTLASGTSWSTMISGAVLGYTATITSSNMPSATGYWVWPISGF
                     TYTGGAIECYIEWFPATAMASPYTTNSFTWQYTTSGNQAMGTSSSTAIPSSQSSWTTQ
                     ARFYNTQIDYTTGPCTGTPSPGNTTTSASPVCPSATFSLGLQNPTTGSGVTYQWQSSP
                     DNITYTNISGATNAAYSLTQSADTWYQCIVTCTNSGQTTTSTPLQVTTNPFYNCYCAS
                     GATSTADEEIFGVTVGSMSNSSTCTTTACGTGSVNSNYSNYKGCVTGPTVSQGDNVPF
                     SLDLGYCGSFAYSNMAAIYIDYNQNGSFADAGEQVYTKPYAAGALPSLVVTGSFIVPF
                     TALTGTTGMRIVYVESSSISSCGTFSWGETEDYNITITASTNCSGTPAPGNTLADQTS
                     VCGGQTINLTLQNSVTGSGVTYDWQSSPDGVTWTSTGGTNPFYSTTQNSATWYQCVVT
                     CTNSGQSATSTAVQVLQNPFMSCYCASGATSTADEEIFGVTVGSMSNSSTCSTIGCGT
                     GSGVGYANYKGCLTGPTVFLGDLVPFSLTLGYCGSYAYSNTAAIYIDYNQNGSFADAG
                     ETVYTKPYGSGTVPSWVVSGNFTVPMTATTGTTVMRIVYVESSTISPCGTFSWGETED
                     YEINIIAPTPCSGTPAPGNTLSDQTTACSNTTINLSLQNIVAALGITYDWQSSPDGVT
                     WTSTGGTQPFYSTTQFTATWYQCVVTCSNSGLSGTSTPVQVTMSPFYNCYCTSGLGGS
                     CSINSLSSVAIATTTLNNVTGGCTGTYNSFPASGNTTASLVPGVPYTMTAGCISGSNT
                     QVAIWIDYNQNGIYETTEYTLINGNIPSGGTGPGSFTIPLTALSGSTGMRVRSDWQGT
                     TAWTSADACTNRTWGETEDYTITVLVPNSCTGTPATSVTTASVASVCPGGSVNLATNP
                     FYTDGGLTFDWQSSTDGITYSSTGVTTQNYNVTGFSVTTWYQCVVTCTNSGLSTTSTP
                     VQVTLNPFYNCYCTANLHSFSTPCIDEIDLNTLSNNTAAAGCALPAYSYQSATTTVLK
                     GSTYTFTRTASGTGVWTGLWIDYNHSGTFDASEYIQVSSTSTATLVNSISLTIPLTAL
                     DGNTGMRIRQRTVNMTATDACTQNFGSGETEDYTINIQTPAPCSGTPAAGNTVSSAPS
                     VCTSVAFNLSISTSYNLAGITYQWQSSTDGITYTNTSGTLPTYSATQSVATWYQCIVT
                     CTNSGLSITTTPVQVTMSPFFNCYCASSATSTADEEIFGVTVGSMTNTSTCTTTACGA
                     GSTNQMYSNYHGCVTPATIIQGTSVPFSLDLGYCGATAYSNTAAIYIDFNQNGLFTDA
                     GETVYTKPYGAGVVPSSVYTGNFTVPMTAVPGTTGMRIVYVESSVVNPCGTYSWGETE
                     DYDIVIAAAAACSGTPAATISGPSNTCAGVPFNLNAGPVGFSGLSYLFQVSTNGGVTW
                     TDLGIASANSIYTISSQTVTSQYQVIVTCSNSSSSDTSAVITVTQNLPSACYCVPTTT
                     GATTYNIINFTTTGGLANINNSSTGTANYENFSAMTATVNPGSSFNYSIDVAGGSNYG
                     RAIWIDFNEDGVFQSTEQVVSSTSYVYPPLTGSISIPLSVTSGTKRMRILASYTPSNP
                     SNACANSGLGQYEDYSLNIMPPVCIFAPTYPADAASGCPDVITNTVPLSWPALSGATG
                     YDVYFGTTNPPTTLVSNNQPGLTFNADVSGGAPIYYWMIVPQISGGGTSCNVWSFTVS
                     PAPVPVASSGGDVCSGNDIFLSSDNVAMGQLTGNTYSWTGPNGFNSTLQNPSISSPSA
                     AYSGTYSVIVTNQYGCTAGASTSLSVNPNPTLTIDSVQNVSCLGGSDGIIYVSASGGL
                     APYSYTSDFIN"
BASE COUNT         2438 a         1466 c         1558 g         2301 t
ORIGIN      
        1 atgtaatata caatatagtc aattgcgatt ttgtaattgt agcaaaacaa caagttttga
       61 tattgcaata agtcatagaa ttaacagaca gtagttatct ttgcagcgaa aattactcat
      121 aacgattatg ctaacagtag atgattttaa ttttaacgga aaacgagctt tagtgcgtgt
      181 tgattttaat gttcctttag acaaagcaac ttttgctgtg actgacaata cacgtattaa
      241 agcagctgtg cctaccataa aaaagatttt gacagatggt ggcagcgtag tactgatgag
      301 tcatttagga cgtccattaa aaaagctgaa agatgatggt actgttgaca caaaaaaatt
      361 tactttaaaa catacggtta gcgaagtgtc gaagttaatg ggggttgatg tgaaatttgc
      421 ttctgactgt attggtgcag aaacatcttc tcttgcctca aaacttcaac cgggtgaagt
      481 gcttttatta gagaatctga gatttcataa aggcgaagaa aaaggcgatg aggaatttgc
      541 caggcagtta gctgcattgg gtaatgttta tgttaatgat gcatttggaa cagcacatcg
      601 tgcacatgcc agcactgcca ttatagccca gttttttcct ggtgcaaaaa tgtttggtta
      661 tgttatggct aatgaaataa aaaatgcaga gcgtattatg cgcaatgctg agaaaccttt
      721 tacagccata atgggtggag caaaggtttc tgataaaata ctcatcattg aacaactgat
      781 gtcacgctgc aatcacattt tgattggtgg tggcatggca tacacttttt ttaaagcaat
      841 gggaggcaac atcggaacat cactttgtga atcggataag ttagatcttg caaaacaact
      901 tttagaacgc gccaaacaat caggtgttca gtttcattta ccattcgatt ctgtacttgg
      961 tgataagttc gataatgatg cacaaacatc tattagtgat aatatgaata ttcctgatgg
     1021 gtggatgggc ttggacattg gacccaaagc aattactgcg ttttcagaaa tcatcagtca
     1081 gagtaaaacc attttatgga atggcccaat gggagttttt gaaatggaaa aatttgctgc
     1141 aggaacaaaa ggtattgctg atgcaattgt taatgcaact tcaaaagggg ctttttcatt
     1201 aattggcgga ggcgattctg cagcagcaat caacaaattt ggtttagctg ataaagtaag
     1261 ttatgtctcc acaggcggag gcgctttatt ggaatatttt gaaggcaaag aattgccggg
     1321 tataaaaatg attcagtcgt aaatttattg taatagaatt aacaaaggtc aatgctaata
     1381 cagcaggaca aatcacacta caattagacc tgagcaaagt agctaaaggt gcatacatac
     1441 tgaatgtaca aaccaatggc agcaacaagc aggtgagagt ggtagtggtg tagaaattaa
     1501 ttttaaattt tataaagcga ggttgttttt atttaagaca acctcgcttt ttttattcaa
     1561 tattttaaaa gtgattttat acttatagat aagagtctat aattacataa ttgttttata
     1621 gacaataatc tatgatttat agattttcat taaaactcct aaaaaaatat cataaaaagc
     1681 tttaggaatt aatctttaat gaataatatt gaaaccgaat taccaaaacc gaacaaatag
     1741 aaaatgattt tttacagtct ttttccgaat tttttagttg ctgaatgcaa atttcggttt
     1801 tgtaaaaaaa atacttgaca acaaaaatca attagtttaa ccaaaatcaa attatcatgc
     1861 taaaaacgca acacaaaaaa gaaagatctg tctcttgttg gagatggata atgtgtttca
     1921 tgtttctgat aggaatgtgg actacgggcg cacatgcgca aacaattaca attggaacag
     1981 gtactactac tactaccggt aatggaactc ctatttatcg ttcgagtagt acaagttctt
     2041 ttcatcacag taagtcaata caattactga ctgcggctca attatcaggt gctggtctta
     2101 ctgctggagc aaatattacc ggatatggtt ataataaaca aaacaacgga caacctgccg
     2161 gtgctaacgg atggacaatt aatgtttatt taaaaaactc atctgctaca accttagcca
     2221 gcggtaccag ttggtccacc atgattagtg gagctgtatt aggatataca gcaactatta
     2281 catcaagtaa catgccttcg gcaacaggat attgggtatg gcctatttct ggatttactt
     2341 ataccggtgg ggcaattgaa tgttatatag agtggtttcc tgcaacagct atggcatctc
     2401 catataccac aaattcattt acatggcaat atacaacttc tggcaatcag gctatgggaa
     2461 caagcagtag tactgctatt ccttccagcc agtcatcatg gactacccaa gcaagatttt
     2521 acaatactca aatagattat acaacaggcc cttgtactgg aacaccttca ccgggtaata
     2581 ctacaacatc agcaagtcct gtttgtccaa gtgctacttt ctctttagga ttacagaatc
     2641 ctacaactgg cagtggcgta acatatcaat ggcaatcatc acctgataat attacatata
     2701 ctaatatcag tggagctacc aatgctgctt attctttaac acaatctgcc gatacatggt
     2761 atcaatgtat tgttacctgt actaacagtg gtcaaactac tacttcaaca ccattacaag
     2821 ttactacaaa tccattttac aactgttatt gtgcttccgg agctacatca acagcagacg
     2881 aagaaatttt tggtgttact gttggatcaa tgagtaattc atcaacctgt acaactactg
     2941 cttgtgggac aggatcggta aactctaact attcaaatta taagggttgt gtaaccggac
     3001 ctacagtttc tcaaggtgac aatgtgcctt tttcattaga tttaggttat tgcggcagct
     3061 ttgcttatag caatatggca gcaatttaca tagattacaa tcagaatgga tcatttgcag
     3121 atgcaggcga gcaagtatat accaaacctt atgcagcagg tgccctacca agcttagtag
     3181 ttacaggaag ttttattgta ccatttacag ccttaacagg cacaacaggc atgcgtattg
     3241 tttacgtaga aagtagctca atcagttcgt gtggtacttt ttcgtgggga gaaacagaag
     3301 actataatat cactattaca gcatctacca actgttcagg tacgcctgca cccggcaata
     3361 cattggcaga tcaaacttct gtttgcggtg gtcagacaat taatttgact ttacaaaata
     3421 gcgttacagg ctcaggcgtt acctacgatt ggcaatcatc acctgatggt gtaacctgga
     3481 ctagtacagg tggaacaaac ccgttttatt caacaacaca aaattcagct acatggtatc
     3541 aatgcgtagt tacttgtacc aatagtgggc agagtgctac ttcaacagct gtacaggttt
     3601 tgcaaaatcc atttatgtct tgttactgtg catcaggtgc aacatctact gcagatgaag
     3661 aaatctttgg tgtaacggtt ggatctatga gcaatagctc aacttgtagc actataggtt
     3721 gtggaacagg ttcaggcgtt ggctatgcaa attataaagg ctgtttaacc ggtcctacag
     3781 tatttttagg tgatttggta cctttctcat taacattagg gtattgtggc agctatgcgt
     3841 atagcaatac agcagcaata tatattgatt acaaccaaaa tggttcgttt gcagatgcag
     3901 gtgaaactgt ttatacaaag ccttatggtt ccggtacagt accaagttgg gttgtttcag
     3961 gaaactttac ggtgccaatg actgcaacaa caggtacaac tgttatgcga attgtttatg
     4021 ttgaaagtag cacaatcagt ccttgtggaa cattttcatg gggtgaaaca gaagactatg
     4081 aaattaatat aatagcacct actccatgta gtggtacacc agcgcctggt aatacacttt
     4141 ctgatcagac aacggcatgt tcaaatacaa ccattaattt atcacttcag aatatagttg
     4201 ctgctttagg tattacctac gactggcaat catctcctga tggtgtaacc tggacaagta
     4261 ccggtggaac acagccattt tattcaacta cccagtttac ggcaacctgg tatcaatgtg
     4321 ttgttacttg ctctaacagt ggattgagtg gaacttctac tccggttcag gtaactatga
     4381 gtccatttta taattgctac tgtactagcg gacttggcgg aagttgctca ataaactcgc
     4441 tttcaagtgt ggcaattgca acaacaacat taaataatgt cactggtgga tgtacgggaa
     4501 catataattc atttccggca tctggaaata caacagcaag tttggttccc ggtgttccat
     4561 ataccatgac tgcaggttgc atttccggta gcaatacgca ggtagctatc tggattgact
     4621 ataaccagaa tggaatttat gaaacaacag aatatacatt aatcaatgga aatattccaa
     4681 gtggaggaac tggaccagga tcgtttacaa ttccattaac tgcattaagc ggctcaacag
     4741 gaatgcgtgt acgtagtgac tggcagggaa caacagcatg gacaagtgca gatgcttgca
     4801 ccaatcgtac ctggggcgaa acagaagatt atactattac agttttggtt ccaaactcat
     4861 gtactggcac acctgcaaca tctgttacaa cagcatctgt tgcaagtgta tgtcctggag
     4921 gaagtgtgaa tttagctacc aatccatttt ataccgatgg tggtttgaca tttgattggc
     4981 agagctcaac tgatggaata acttattctt cgacaggtgt tactacacaa aattacaatg
     5041 taaccggatt ctctgtaact acatggtatc aatgtgttgt tacttgcacc aacagtggat
     5101 taagtactac ttcaactcct gttcaggtta cattaaatcc attctataat tgctattgta
     5161 ctgcaaacct gcactcattc agtacgcctt gtatagatga aattgattta aatacattga
     5221 gtaataacac tgcagcagca ggatgtgcgt tgcctgcata ttcctatcag tcagctacaa
     5281 caacggttct aaaaggaagc acatatacct tcacaagaac agcgtcagga actggagtat
     5341 ggacaggctt atggatagac tataatcata gtggaacgtt tgatgcaagt gaatatattc
     5401 aggtatcgtc aacttctaca gcaacattgg tcaacagtat ttcattgacg ataccactta
     5461 ctgcattgga tggcaataca ggaatgcgta tccgtcaacg cacagttaat atgacagcaa
     5521 ctgatgcttg tactcagaat tttggatcag gtgaaacaga agattacaca atcaatattc
     5581 aaacaccagc accatgtagt ggaacaccag cagcaggaaa tacagtttcg tctgcacctt
     5641 cagtttgtac aagtgttgca tttaatcttt caatatctac aagttataat ttggcgggaa
     5701 ttacttatca gtggcagtcg tcaacagatg gaattactta taccaatact tcaggaacat
     5761 tgccaaccta ctcagcaact caatctgtgg caacatggta tcagtgtata gtaacatgta
     5821 ctaatagtgg tctgtctata acaactactc ctgtacaggt aactatgagt ccattcttta
     5881 attgctattg tgcttcttca gctacttcta cagccgatga agaaatattt ggtgtgacag
     5941 ttggttctat gactaataca tctacttgta caaccactgc atgcggagca ggttcaacta
     6001 accaaatgta ttctaactat catggttgcg taacaccggc tactatcatt cagggaacat
     6061 cagtaccttt ctcactcgat ttaggttatt gcggtgcaac tgcatatagt aatacagcag
     6121 caatttatat tgacttcaac caaaatggtt tgtttacaga tgcaggcgaa acagtttata
     6181 ccaaacctta tggtgcagga gttgtgccaa gttcggttta tacaggcaat tttacagttc
     6241 cgatgacagc tgttcccggt acaacaggaa tgcgtattgt ttatgtagaa agtagtgtag
     6301 taaatccatg cggaacatat tcatggggtg agacagaaga ctatgacata gttattgcag
     6361 cagcagcagc atgctccgga acaccggcag caacaatcag tggtccttca aacacttgtg
     6421 caggcgttcc ttttaacctt aatgcaggtc ctgtgggctt tagtggatta agttacttgt
     6481 tccaagtttc tactaatggt ggagttacat ggactgattt aggtattgct agtgctaata
     6541 gtatttatac catcagcagc caaacagtta ccagtcagta tcaggtaata gttacttgtt
     6601 ctaacagcag cagtagcgat acatcagctg ttattactgt aacacagaat ctccctagtg
     6661 catgttattg tgtacctaca acaactggag caacaaccta taacatcatt aactttacaa
     6721 caacaggtgg tttggcaaat attaacaaca gcagcacagg tacagcaaat tatgagaact
     6781 tcagtgcaat gacagcaact gttaatccag gttcatcatt taactatagt attgatgtag
     6841 ctggaggtag taattatggt cgtgccatct ggattgactt taacgaagat ggagtattcc
     6901 aatcaacaga gcaggtagta agtagcacat cttatgttta cccgccactt acaggaagta
     6961 tatcaattcc attaagtgta acttcaggaa caaaacgtat gcgtatcttg gcatcatata
     7021 ctccaagtaa tccatctaat gcatgcgcaa acagcggttt gggtcagtat gaggattata
     7081 gtctgaacat catgccaccg gtatgtattt ttgcaccaac ctatcctgca gatgcagcaa
     7141 gcggatgtcc tgatgtaatt accaatactg ttccattaag ctggcctgca ctttcaggag
     7201 ccacaggcta tgatgtgtat tttggtacca caaatcctcc aactactttg gtttctaata
     7261 atcaaccggg tttaactttc aatgccgatg tatcaggtgg agcaccaatc tattactgga
     7321 tgatagtacc acagatatca ggtggaggta cttcatgtaa tgtatggtca tttactgtat
     7381 caccggcacc tgttccagta gcaagttcag gaggcgatgt atgctcaggt aatgatatct
     7441 tcctttcatc tgataacgta gcaatgggac aacttaccgg caacacctat tcatggacag
     7501 gacctaatgg ttttaacagc acattacaaa atccatctat cagcagccca agtgcagcct
     7561 acagcggtac ctattcagtt atagttacaa atcagtatgg ttgtacagca ggagcaagca
     7621 caagtctgag tgtaaatcca aatccaacgt taacaataga ttctgtacag aatgtaagtt
     7681 gtttaggcgg aagtgatggt attatttacg tatcagcgtc aggcggctta gcaccttata
     7741 gttatacttc agattttatt aac
//
LOCUS       DAOOOI010000087         7757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1853_length_7757_cov_5.868476, whole genome shotgun sequence.
ACCESSION   DAOOOI010000087 DAOOOI010000000
VERSION     DAOOOI010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1853_length_7757_cov_5.868476"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PKX59_09230"
     CDS             <1..729
                     /locus_tag="PKX59_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91087.1"
                     /translation="RTCCYDIYDGVYLDTVKQVKKDTLIAIEPIASDITKDSITIEVI
                     KQIEPLLPMKLYFENDQPNPKTMLTTTKSNYVDLYNAYLTNEKKYRENYVDVLTDESQ
                     RNKAAAEITTFFNQVVKKNYLDLDRFSDLVLKQLDAGNKIEIIVRGSASPLAESGYNK
                     NLSKRRISSVINFWRVYDSGKLNNYIKAGKLTITEDAVGEEMTTTKISDDLKDKRNSV
                     YSPAAGSMRYIQVMRVMINGVAVE"
     gene            800..>1528
                     /locus_tag="PKX59_09235"
     CDS             800..>1528
                     /locus_tag="PKX59_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ91088.1"
                     /translation="MSNEISQKISHLREQAEALMTVNTAKAIILLSEAMNHIDADDEK
                     LKSECGLQLALAYHKVKEELMAIKIINQCLELKVNSDSLKNQIPFNELAAEIYTAISI
                     YDSALEHLLNIASAYTSAKDKQKLGLVLNQIGETHKLLGGYNEAIRHHEKALKIFEEL
                     DNKEQLSITNFYIGNCYNWADELDIAFNYLDTSLKIAEKLRSPELKIKTLGSLAILFT
                     KQNKFEKSLDYFFQAIDNSNLAGNV"
     assembly_gap    1529..1575
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1576..3350
                     /locus_tag="PKX59_09240"
     CDS             <1576..3350
                     /locus_tag="PKX59_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ91089.1"
                     /translation="GNLYNQMHRHDEAIKILLEADEICQSLNIKQPLHLVNLFLSDAY
                     AALNNFEKSLAHHKLYYKLCREIQSEDVSHRTKSLQLKYDLEEIQREKSKAEESLLLK
                     DTFLANVSHELRTPLNGIIGMADLLTETVTTDENRKYVQTILSSAFQLSNIVDEILDY
                     ARLNTGQVSPVEHPFILNDTIKECLDKFLLKFPGKKVESDTDFAACDEVLLDEGILKK
                     VVFNTLVFGGSFASNDKLELALYHSQNKIFINVKFNIVNKKSFPHPDNFFELNNPQFS
                     QNGLNGYGLGLSLINARGMIVACGGKISVKLESSKCNFVIELPYKAVKSDRKNIVSEP
                     PAATEDVTILLVEDNKINQFLAKTMLLKKGYKVELAADGDEALTLLRKGNFSMVITDV
                     QMPGMDGYQLAGHIRSKLPDPINTIPILALTAYESSAEKEKAKAAGMSGFLTKPYMPE
                     QLFDLITQHLPLKNDQSKDAVEYESTYNVNEIYDNLLALMAGDKKETASLIKMLQVQF
                     PELLSEMLQAIEDKDMKKLYKAAHKIKSSLKIFNNNSVGLLIDQVERKAKDNEYSFEL
                     SVKVSELIKQVNVIIGSLDQLIKK"
     gene            complement(3361..3912)
                     /locus_tag="PKX59_09245"
     CDS             complement(3361..3912)
                     /locus_tag="PKX59_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HOZ91090.1"
                     /translation="MKQILMNEKLTFRVVAIISVVVLGMVMLLSQKVLPRPETPPAFT
                     KYLPGLNAFVNGTCFVLLLISFYFIKKKRIDIHKKINLLTFLLSAVFIVSYFLYHYLA
                     EETKYPVDHPLRGLYLFILASHIILAAIVMPLVLLSFYYGLKMDVRKHRKLTRFSFPI
                     WLYVTLTGVIVYLMISPYYSFNQ"
     gene            complement(3914..4507)
                     /locus_tag="PKX59_09250"
     CDS             complement(3914..4507)
                     /locus_tag="PKX59_09250"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOZ91091.1"
                     /translation="MPLFFFILMKEGKHNFRYLEYYGPKHLATNGVDTIYHSISDFSL
                     KNTEGNTVSPSNTEPSYWVFQFFNASCQEPCKEVFTNMYDVARDFVTAPKVKFVSITT
                     QPFNDSLPVMKHVMKMNSIHPPQWQLCTGDSLGIDSLMRTSFFLKGNTNAALNSVFLV
                     DSKLHLRGIYDGADLLEMKRLKDDIKILSYETRQKSK"
     gene            complement(4561..4926)
                     /locus_tag="PKX59_09255"
     CDS             complement(4561..4926)
                     /locus_tag="PKX59_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HOZ91092.1"
                     /translation="MSHNEHNEHKPHGYYTGDIHNQFVEEHYDPTAKKIIWRTFWILL
                     VATIVEVAIAFIPMHKDLLQWIFVALTIFKSAYIVGVFMHLGKEKKSLQYTILLPFFL
                     IVYMIAICLIEGVYLFHIG"
     gene            complement(4951..5766)
                     /locus_tag="PKX59_09260"
     CDS             complement(4951..5766)
                     /locus_tag="PKX59_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HOZ91093.1"
                     /translation="MSTATEAKSKSIWGGGISPFNVSWGKMFMWFFLVSDAFTFSTLL
                     VAYGTMRHRFTDIWPAPGDVFTHFPFIHEHVPLAYVGLMTFILIMSSVTMVLAVDAGH
                     QRNHKSVAMWMGLTIVGGLMFLGSQAWEWYHFIHGSKEGTYILADGRFARTDDYEPGK
                     LFFWHNHQKTVVEGAEAEKLKASAVRNIKGANMTENEYGNTPLFANFFFFITGFHGFH
                     VFSGVVINIIIFINVLNGVYKRRGHYEQVEKSGLYWHFVDLVWVFVFTFFYLV"
     gene            complement(5900..6475)
                     /locus_tag="PKX59_09265"
     CDS             complement(5900..6475)
                     /locus_tag="PKX59_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HOZ91094.1"
                     /translation="MQTVTTEQDNNYTGIPTAKILLWIAMASMVMLFAGLTSAYIVRK
                     NEGNWIEFSLPKMFTISTITIIISSFIIQYAIYAIKRNLLNQVKIAVITTLGLGIAFV
                     FMQFYAWTVMVNNGIHLVGNPSGSFIYVLSGMHILHLAGGIISMIVISVKSILERYSP
                     KNYLGLQLGAIFWHFLDILWVYLFVFVTFIA"
     gene            complement(6481..7368)
                     /gene="cyoE"
                     /locus_tag="PKX59_09270"
     CDS             complement(6481..7368)
                     /gene="cyoE"
                     /locus_tag="PKX59_09270"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HOZ91095.1"
                     /translation="MLKTTMVVAPRTFTARLVDYGQLFKLRLASLVVLSAALAFLIGS
                     TTIDYTKLWLLVLGGFLVTGSSNALNQIYERRTDKIMKRTRMRPLPDSRMSLTESLVV
                     TIAAGLLGLFILTYYINLYSGILGLIALVSYAFAYTPLKGVTPLSVFVGAFPGAIPTL
                     LGWVAATGQFSYQGWILFSIQFMWQFPHFWAIAWKLYDDYMSAGFQLLPSGGKNSASA
                     FQTTIYTFTLIPIGLLPYYSGMSGIVSAVVITLAGILFFMQSVYLLKTCSDIAAKRLM
                     FGSFIYLPVVLIAILLDKI"
     gene            complement(7533..>7757)
                     /locus_tag="PKX59_09275"
     CDS             complement(7533..>7757)
                     /locus_tag="PKX59_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91096.1"
                     /translation="VFEFYYFCKKLLNMLNSIWHGIAAFFEFIFSLAKPIGKSVDIVF
                     MVLIAIGLLYWLIYEQRVMGGKPATILLIK"
BASE COUNT         2696 a         1330 c         1426 g         2258 t
ORIGIN      
        1 cgcacttgtt gttatgatat ttatgatggt gtttatttag ataccgttaa acaagttaag
       61 aaagatactt tgattgctat tgaacctatt gcttcagaca ttacaaaaga ttctataact
      121 atagaagtca tcaaacagat agaaccattg ttaccaatga agttgtattt cgaaaacgat
      181 caacccaatc caaaaacaat gttgacaaca acaaaatcaa attatgttga cttgtacaat
      241 gcatatctta caaatgaaaa aaagtacaga gagaattatg ttgatgtttt aactgatgaa
      301 tcgcaacgta ataaagctgc tgcagaaatt actacattct ttaatcaggt tgttaagaaa
      361 aactatcttg accttgaccg tttcagcgac ttagtactga agcagcttga tgcaggaaat
      421 aagattgaaa taattgtacg aggttctgca agtcctttgg cagaaagtgg ctacaataaa
      481 aacctctcaa aacgaagaat cagcagtgta attaacttct ggagggttta tgattccggc
      541 aaactgaata attatatcaa ggcaggcaag ttaaccatta ctgaagatgc cgtaggcgag
      601 gaaatgacca caacaaaaat cagtgacgac ctgaaagata aaagaaactc ggtttacagt
      661 cccgctgcag gtagtatgcg ctacattcag gttatgcggg taatgataaa cggtgtagct
      721 gtagaataat gatttttata tatttatagt ggcaaccctg ccattttatg cttacttttg
      781 ctcctgaaaa cagaaaaaat tgagtaacga aatttcacag aaaatcagtc atctgcgcga
      841 gcaggccgag gcattaatga cggtaaatac cgctaaagct attattcttc tcagcgaagc
      901 catgaatcat attgatgctg atgatgaaaa gctcaagtca gagtgtggac tgcagttggc
      961 attggcttat cacaaggtga aagaggagtt gatggccata aaaataatca atcaatgtct
     1021 tgagttaaaa gttaacagtg atagcctgaa gaatcagatt ccttttaatg agctggccgc
     1081 agaaatttat acagcaattt ctatttacga tagtgccctt gagcatttac tgaatattgc
     1141 ctcagcctat acttcagcaa aagacaaaca aaagcttggt ttggtgttaa atcagattgg
     1201 tgagacccat aaattactcg gtggttataa tgaggctatt cgccatcatg agaaagcatt
     1261 aaaaattttt gaagagcttg ataataaaga gcagctttca ataactaatt tctatattgg
     1321 aaattgttat aactgggctg atgaattgga tattgctttc aattatcttg atacaagttt
     1381 gaagattgcg gaaaaacttc gtagtcctga attaaaaata aagacattag gtagcctagc
     1441 aattttgttt accaagcaaa ataaatttga gaaatcgctc gactattttt ttcaagctat
     1501 tgataacagt aaccttgcag gcaatgtann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnttggt aatctttata atcagatgca tcgtcatgat gaagctataa
     1621 aaattttgct tgaggcagat gaaatctgtc aatcacttaa cattaaacag ccattacatc
     1681 tggttaattt gtttttatct gatgcctatg ctgcattgaa taatttcgaa aaatcgttag
     1741 cacatcataa gttgtactat aaactctgtc gtgaaataca gagcgaagat gttagccata
     1801 gaactaaaag cttgcaactg aagtatgacc tggaagagat acagcgagag aagagcaaag
     1861 cagaagaatc acttttacta aaagatacat ttcttgctaa tgttagtcat gagttgagaa
     1921 ctccgcttaa tggaattatt ggcatggccg atttgttgac ggaaactgta acaacagacg
     1981 aaaacagaaa gtatgttcag actattttgt catcagcatt tcaattatca aatattgttg
     2041 acgaaatatt agactatgca cgtttaaata caggacaggt tagtccggtg gagcatcctt
     2101 ttattttaaa cgatacgatt aaagaatgcc tggacaaatt tttattgaaa ttccccggaa
     2161 aaaaagttga atctgatact gattttgcag cttgtgatga agtgttgctt gatgaaggta
     2221 ttcttaaaaa agttgtattt aataccttgg tctttggtgg ttcttttgca agtaacgata
     2281 aacttgaact tgcattatat cattcgcaga acaaaatttt tatcaatgta aaattcaaca
     2341 ttgtaaataa aaagagtttc ccgcatcccg acaatttttt tgaactcaac aatccgcagt
     2401 tttcgcagaa tggattgaat ggatacgggt tagggttgtc actgataaat gccagaggaa
     2461 tgattgttgc ttgcggagga aaaatttcag taaaacttga aagttcaaag tgtaactttg
     2521 tcattgagct accttataag gctgttaagt cagacagaaa gaatatagta agtgaacctc
     2581 ctgcggctac agaagatgtg actattttat tggtagaaga caataaaata aatcagtttc
     2641 ttgccaaaac aatgttgctg aaaaaaggct ataaagttga acttgcagca gatggcgatg
     2701 aggcacttac tcttcttaga aagggtaatt ttagtatggt aattacagac gttcagatgc
     2761 ccggaatgga tggctaccaa ctggcagggc atatacgaag taaactacct gatcccataa
     2821 atacaatacc tatccttgca ttgacggctt atgaatcgag tgcagagaaa gagaaggcaa
     2881 aggctgcagg tatgtcaggt tttttaacga aaccctacat gcctgagcaa ctgtttgatc
     2941 tcataacaca acatcttcca ttgaaaaatg atcaatcaaa agatgcagtt gaatatgaat
     3001 caacttataa tgtcaatgaa atttatgaca accttttggc tttaatggca ggagataaaa
     3061 aagaaacagc ttcattgata aaaatgcttc aggtacaatt tcctgaatta ctcagtgaaa
     3121 tgttgcaggc tattgaagac aaggatatga agaaactgta taaggctgcg cataagataa
     3181 aatctagtct gaaaatattc aacaacaatt ctgttgggtt attaatagat caggttgagc
     3241 gcaaggctaa agacaatgaa tattcttttg aattgtcagt taaagtgtct gagcttatta
     3301 agcaggtgaa tgtaattatt ggttcactag accaactaat aaaaaagtaa acagaaaatt
     3361 ttattggtta aacgaataat agggtgatat catcagataa acaatcacac ctgttaatgt
     3421 aacatacaac cagataggga aagaaaaacg tgtaagctta cgatgtttac gcacatccat
     3481 ttttaaacca taataaaacg ataacagtac caatggcatc actatggcag caagaataat
     3541 gtgcgatgct aaaatgaaca gataaagtcc gcgcaacgga tggtcaacgg gatatttggt
     3601 ttcttctgct aaataatgat atagaaaata agatacaata aaaacagcac tcaataaaaa
     3661 tgtaagcagg ttaatttttt tatgaatgtc tatgcgtttc tttttgataa aataaaatga
     3721 aattagcaac aggacaaaac aagttccgtt tacaaacgca tttaaaccgg gaaggtactt
     3781 cgtaaatgcc ggaggcgttt caggacgtgg aaggactttc tggcttagta gcataaccat
     3841 tcctaataca acaactgaaa taattgctac tactctgaaa gtaagctttt cattcattaa
     3901 tatctgtttc atcttacttt gatttttgtc ttgtttcgta actaagaatt ttaatatcat
     3961 ctttcaatcg tttcatctct aataaatcgg caccgtcata gatgccgcga agatgcaact
     4021 tactgtcaac caaaaataca gagttaagtg ctgcattggt gtttcctttc aggaaaaatg
     4081 atgtgcgcat taaagaatct attcccaatg aatcgcctgt acataactgc cattgcggag
     4141 gatgaatgct gttcattttc ataacatgtt tcattacagg taaactgtcg ttaaatggct
     4201 gtgtcgtgat ggaaacaaat ttcactttag gtgcagttac aaaatcgcgt gccacatcat
     4261 acatattggt aaaaacttct ttgcacggtt cctgacaaga agcattaaaa aactgaaata
     4321 cccagtaaga gggttcggtg ttgcttggtg aaacagtatt tccttctgta ttttttaacg
     4381 agaaatcaga tatggaatga taaatggtgt caactccgtt tgtagccaga tgctttgggc
     4441 cataatattc aagatatcgg aaattatgtt tcccttcttt catcagaata aaaaaaaata
     4501 aaggcatcag aagtattaaa aaaaccgctg ctgccttaaa ctttttcaag ataaaaaagc
     4561 ttatcctata tggaacagat atacaccttc aataagacaa attgcaatca tgtaaacaat
     4621 cagaaaaaat ggtaagagaa tggtgtactg aagactcttc ttttcttttc caaggtgcat
     4681 aaaaacacct actatatatg ctgacttaaa aattgttaat gcaacaaata tccattgcaa
     4741 caaatcttta tgcattggaa taaaggctat tgctacttca acaattgttg caacaagcaa
     4801 tatccagaat gtacgccaga taattttttt agccgtaggg tcgtaatgct cttctacaaa
     4861 ctgattgtgt atatcaccgg tgtaataacc gtgtggctta tgctcattat gttcgttatg
     4921 actcatgatt ctatttttct taaagattag ttatacaaga tagaagaagg tgaatacgaa
     4981 tacccaaacc aaatctacaa agtgccaata aagccccgat ttttcaacct gctcataatg
     5041 accgcgcctt ttgtaaacgc cattgagcac attaataaaa attattatgt tgatgactac
     5101 accactgaaa acgtgaaaac cgtgaaaccc tgtaataaag aaaaagaagt tggcaaataa
     5161 gggggtgttg ccatattcat tctcagtcat gtttgcacct ttaatgttgc gcactgcaga
     5221 tgctttaagt ttttctgctt cggcaccttc aacaacagtt ttctgatggt tgtgccagaa
     5281 gaataatttt ccgggttcat aatcgtctgt gcgtgcaaaa cgaccatcag caagaatata
     5341 agttccttct tttgaaccat gaataaagtg ataccactcc catgcctgac tgcctaagaa
     5401 catcaaaccg ccaacaatgg ttaatcccat ccacatggca acacttttat ggttgcgctg
     5461 atgtcctgca tctactgcaa gtaccattgt taccgaactc atgataagaa taaaggtcat
     5521 taaacctaca taagctaaag gtacatgttc gtgtataaaa gggaagtgcg taaacacatc
     5581 acccggtgca ggccatatat ctgtaaaacg atggcgcatg gtaccataag ccaccaggag
     5641 ggttgagaat gtaaaagcat cagataccag gaaaaaccac ataaacattt ttccccagct
     5701 tacattgaat ggtgaaattc caccacccca gatggatttt gattttgctt cagttgctgt
     5761 tgacatatta agttaggagt cttgaaatta atttaacagg atgcaaatat ttaatttttt
     5821 ttccaattca tcaatttatt gtaaaatttt tggccattta accataattc attgacagat
     5881 tggagcaaaa gatagccttt caggctatga aggtcacaaa tacgaagaga taaacccata
     5941 ggatatcaag aaaatgccag aaaatggcac ctaactgaag tcctaaatag tttttgggag
     6001 aatatctttc aagaatagat tttactgaaa taacaatcat ggaaattatt cctcctgcaa
     6061 ggtgcagaat gtgcattcct gacaatacat atataaaaga acctgatgga ttacctacta
     6121 aatggatgcc gttgtttacc ataactgtcc aggcataaaa ctgcataaag acaaaagcaa
     6181 tacccagccc gagagtggtt atgactgcaa tttttacctg attgagcaga tttcttttaa
     6241 tagcataaat agcatattga attataaaac tgctaataat tatggtaata gtgctgatgg
     6301 taaacatctt tggtaaacta aactctatcc agttgccttc gtttttacga acgatatagg
     6361 cacttgtcaa accggcaaat agcataacca tactggccat ggctatccac aataatattt
     6421 tagctgtggg aatgcctgtg taattattat cctgttctgt ggtgactgtt tgcattgtaa
     6481 ttaaatttta tcaagtaata tggcaattaa tacaacgggg agatatataa atgaaccgaa
     6541 catcagcctt tttgctgcaa tgtccgaaca tgtttttaat aaataaacac tttgcataaa
     6601 aaataaaata cctgcaaggg taatgactac tgcagataca attccactca tgccactgta
     6661 gtatggcagc agaccgattg gtataagtgt aaaagtatat attgttgttt gaaatgcaga
     6721 agcagaattt ttaccacccg aaggcagcaa ttgaaagcct gcactcatat agtcgtcata
     6781 aagtttccag gcaatagccc aaaagtgtgg aaactgccac atgaattgaa tagaaaaaag
     6841 tatccatccc tgataactga actgaccggt tgcggctacc caaccaagca gggtaggaat
     6901 agcacccgga aacgctccaa caaaaactga taatggtgta acacctttta atggtgtgta
     6961 agcaaaagca taacttacta aagcaatcaa gccaagtatt ccgctgtaaa gattaatgta
     7021 gtaggtgagg ataaacaatc ctaataaacc tgctgcgata gttacaacaa gcgattctgt
     7081 aagcgacatt ctgctgtcag gaagcggacg cattctggtg cgcttcataa ttttatcggt
     7141 gcgtctttcg taaatctgat tcaatgcatt tgaagaaccc gtaactaaaa agccacctaa
     7201 aacaagaagc cacaacttag tgtaatctat cgttgttgaa cctataagaa aagctaatgc
     7261 agcagagaga actactaacg aagccagcct tagtttaaac aactgtccat agtcaacaag
     7321 tctggcagta aaggtacgtg gtgcaacaac cattgttgtt ttcaacattt aagcaaaatg
     7381 aaaaaatttc ggaatgcaaa ggtataaaat tgatggtaag gaagcttgaa aatgctaatt
     7441 acttactgat aaacataaaa aaaggcagcc aaaggctgcc ttaaaattct tttctatgaa
     7501 gtaaatgtca aatggttatt tattcttttc cttcacttta tcagcaagat agttgcgggt
     7561 tttccaccca taactctttg ttcgtagatg agccaatata acaatccaat ggcaattaat
     7621 accataaaaa ctatatctac agattttcca atgggttttg ccaagctgaa aataaattca
     7681 aaaaatgctg ctataccgtg ccagattgag tttaacatat tcaataattt tttgcaaaaa
     7741 taataaaatt caaatac
//
LOCUS       DAOOOI010000088         7547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1919_length_7547_cov_8.870529, whole genome shotgun sequence.
ACCESSION   DAOOOI010000088 DAOOOI010000000
VERSION     DAOOOI010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7547
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1919_length_7547_cov_8.870529"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="PKX59_09280"
     CDS             <1..1379
                     /locus_tag="PKX59_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91097.1"
                     /translation="FIQGVQPSFDLGINKISTSPYLAIGNNDIVGIIKNYGSSTINSV
                     KVNYSINGGAVASDSLTGISIAPLATYTFTHSIPWTSTAPGAYTVNCYATEINGSNAD
                     QNNVNDSASKVLNILLSIEQRIPLFEIFTGSTCPPCLPGNTNFHGIVDTIDQNNFVSL
                     KYQQNFPGTGDPYTTVESTNRRSTYYGINSIPRMENDGGWDGNANSFTYALYQDARAV
                     PASFILNGTFTSDTINRTYSAKIKYSPLFNSTSTRLNVVIAEKTTSLNVKTNGETAFF
                     HVMKKMLPDETGTLLPSMTAGTWDSTTITYSFNGNYRLPTNGQAANIIDNSFEHSVEE
                     FADLTMMGWLQADDGTKQVFQAANFVEEMITGIYSMNKSVNSILIYPNPAKEVASVEI
                     MLTASEKLSLKLMDAEGRTLEIKQVNGQTGINKAQFDVSQLAAGYYHIAVSDAKNNSF
                     VRRIVVVK"
     gene            1556..2008
                     /locus_tag="PKX59_09285"
     CDS             1556..2008
                     /locus_tag="PKX59_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91098.1"
                     /translation="MAKLKVENKEMLSYLLFDADNPLEYAQGIKQEIQLQFKELNVHY
                     YYATKSLRKTLRLINKYSRFTKFKQGEIELLLHFSALFSNTIASDTKHLPLLGLQYRS
                     LTKAASLIQKIHEDLRYDYVKEFNLILEKTKSSFKNWDAQKFMVQELD"
     gene            2464..3033
                     /locus_tag="PKX59_09290"
     CDS             2464..3033
                     /locus_tag="PKX59_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91099.1"
                     /translation="MKRSILIYILLHICIHSYSQSWNWAKHIHSGGGERATVSCIDGQ
                     GNSYTIGGFDSNYSCQFDTVTLFGVGTSTSFLAKYESGGNLVWVKKISYTSSQNCSGG
                     IVDLVYDSISNSLLFTGSICGSFQMNSTIITSSFGSMDAIFGKADLNGDILWYETITG
                     LGIKQGRTIAYDNAGSVYVAGTNTHPIQI"
     gene            3049..4110
                     /locus_tag="PKX59_09295"
     CDS             3049..4110
                     /locus_tag="PKX59_09295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91100.1"
                     /translation="MGTFLVKYTSSGVQQWAKNISGRGKYIASIAFDPRDMQVKGNSL
                     FLTGSMWRDTLNIDTVHIAIPNAYGTIVSKFDLNGNVAWAKAFGYPMGGDIGYSLAVD
                     SNSNVYAISAFYGDSAIFGDTVIFNSGMRDMYFVKYKSNGDFSWVRQTHSTLGAEGFS
                     VASDAMDNLYITGKFSGTAQIGNFQVVSMGANDMFLAKYAPNGDCIGVDNFGYAEGIS
                     VVVDNNNNPIVSGIFKNTVTIGNTSYTCNDVFDVFIAQHVAITNVKNQLKSNSNQLLI
                     HANPNTGKCNIRVPDEMMHEKNLVLSIYNSQGKVIQQIPVNTNEEKIKINLEAEATGI
                     YNAVLSNGNKTYSGKIVFE"
     gene            4288..4758
                     /locus_tag="PKX59_09300"
     CDS             4288..4758
                     /locus_tag="PKX59_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91101.1"
                     /translation="MYLTLSRNTVLFIGIAILSFYYCLNKIQFILDAEITYGTVSSSS
                     EGNNRETSTIFFSTKDSNYFFGATENVHYTLNQTVAVIYKSEKPQQAFVFSFLGFWYE
                     GLLYCLIPLCLWCAFLLSFYSSSDSFILPFFAKSSVKQQAKNSTKDSAVTKQSP"
     gene            complement(4742..5743)
                     /locus_tag="PKX59_09305"
     CDS             complement(4742..5743)
                     /locus_tag="PKX59_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91102.1"
                     /translation="MNEVLKEHPYVKSIIQLSDAEDDRYVFHQKVAPILVQMGNDKEF
                     LKEVVRRNLTDEGYLSQTWTLYNIPYFFVYENEHINIKIHLFPRHERKLEGIAAHCIH
                     HHNNYLLTSNAFFGSGYESMLFEKELNLNEKDLTVSMKIRKHFHQKEWNPSLVDSWEP
                     HVVFIPEKLSATLVIWTPDKKRNTDNLRANPILKAVKGPLRWAIHAFGLTEKFGIAAK
                     NTYQFYPEKGKSTFKAIEENEYFAPTRAAKGAEVDEYSMRMIFDFLQQADLVEPEFLK
                     QVRANTITPAYYYKWLDMVLNNQKVPEVFHREEINIPIRAYTRADIQTAAGLVKETA"
     gene            complement(5916..7379)
                     /locus_tag="PKX59_09310"
     CDS             complement(5916..7379)
                     /locus_tag="PKX59_09310"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOZ91103.1"
                     /translation="MKTAEAKNDYENKLTTWIESEKAAVELINVIGNLWFDKSVELII
                     FRNQLVDRSASEILNLHLYALNIIKQPINVQDTLLLAKEIAKLRNIAPSRIDIGRLGA
                     EWLVEKKLGTNATHFIEDKLAGFLGSEKKTIKPKDVILYGFGRIGRLAARELITQAGK
                     GEQLRLRAIVTRSSSDEDISKRADLLRTDSVHGPFPGTVVEDLENKAIVINGHSVLML
                     SGKNPEDLDYTAYGIDNALLIDNTGVARDREGLSKHLKAKGVAQVLLTAPGKGDIPNI
                     VYGVNHESYDSKETIFSAASCTTNAIVPVLKVIEESLGIERGHIETIHSYTNDQNLLD
                     NYHAKYRRGRSAALNLVITETGADKAVAKVLPHLAGKLTGNAVRVPTPNVSLAILSLN
                     IKKTAAKEEVNAIMKEAALKGDLVEQIQYSFSNELVSSDLVGNPCSSIFDSPATIVSK
                     DGKNVVLYVWYDNEYGYTRQVMRLAKQLSGVVRLRYY"
BASE COUNT         2324 a         1456 c         1412 g         2355 t
ORIGIN      
        1 aatttataca aggagttcaa cccagttttg atttagggat aaataaaatt agcacctcgc
       61 cctacttggc gattgggaac aatgacatag ttggaataat taaaaattat ggttcttcta
      121 ccataaattc cgtaaaggta aattacagca ttaatggtgg tgctgttgcc agtgattcct
      181 taaccggaat ttcaattgct cccttggcta cctatacctt tacacacagc attccttgga
      241 cttcaaccgc tccaggggca tacactgtaa attgctatgc cactgaaatc aatgggtcca
      301 atgccgacca aaacaatgtg aacgacagtg catcaaaggt tttaaatatc cttttgtcaa
      361 ttgagcaacg cattcctctc tttgaaatct ttacagggtc cacttgtccg ccttgcttgc
      421 cgggcaacac caattttcat ggcattgtag ataccattga tcaaaacaat tttgtatccc
      481 taaaatacca gcaaaatttt cccggcacag gagatcctta cacaactgtg gaaagtacca
      541 atagacgctc cacctattac ggcataaatt cgattccccg catggagaat gacggaggtt
      601 gggatggaaa tgccaattct tttacctatg ccctttatca ggatgcacgg gcagttccgg
      661 cttcctttat tttaaatgga acgttcacct ccgataccat caaccgcact tactcagcaa
      721 aaataaaata ctcacccttg tttaattcta cctctactcg actgaatgtt gtaattgctg
      781 aaaaaacaac ctccttaaat gttaagacaa acggggagac agcttttttc catgtaatga
      841 aaaaaatgct acccgatgaa accggaacac tcttaccaag catgacagcc ggtacttggg
      901 attcaaccac cattacgtat tcttttaatg gaaattaccg tttacccacc aatggacaag
      961 cggcaaacat tattgacaat agttttgagc attcggttga agaatttgcg gatttaacca
     1021 tgatgggatg gttgcaagcg gatgatggta ccaaacaagt gttccaggct gccaactttg
     1081 tggaagaaat gattaccgga atttactcta tgaacaaaag tgtcaactcc attttgattt
     1141 atcccaatcc agctaaagag gtggcctcag tcgaaattat gttgaccgct tctgaaaaat
     1201 taagcttaaa attaatggat gcagaaggtc gcaccttgga aataaaacaa gtgaatggac
     1261 aaaccggcat aaataaagct caatttgatg tgagtcaact ggcagcaggg tattaccata
     1321 ttgcggttag cgatgccaaa aacaattcct ttgtacgaag aattgtggtt gtaaaataat
     1381 ctaacttgac tatttaaaag ccgctttgtt gcatgcaaag cggctttttt attttaattt
     1441 gtactattgt aaaattgcta atgcagataa ggatttggaa aaagaaaaga tacatttact
     1501 caaaaaggaa cttagccagc aaggacataa ggaattggta gagatttgtt tgaacatggc
     1561 aaagttaaag gtagagaaca aagagatgct tagctacctt ttgtttgatg cagacaatcc
     1621 gctggaatac gctcaaggca ttaagcaaga aattcaatta caatttaagg aattaaatgt
     1681 gcattattac tatgcaacaa aatcattgcg caaaacactc cgcctgataa ataagtacag
     1741 ccgttttaca aaatttaaac agggcgaaat tgaattgctg ctgcattttt cagcactttt
     1801 ttcaaacact attgcaagtg ataccaaaca tttaccattg ctgggattgc aataccgtag
     1861 cctgacaaaa gcagcctcgc tcattcaaaa aattcatgag gatttgcgtt atgattatgt
     1921 aaaggaattc aatttgattc ttgaaaaaac aaaaagcagt tttaaaaact gggatgctca
     1981 aaaatttatg gtacaagagc ttgattagtt caaaaaatca ttttttattt ccattactat
     2041 ttctacatta gatatatcaa caaaatagtt ttctgcaaca attccttcaa attactaagc
     2101 acattcccat acagcagaaa cggtgcaaaa gatagagccg aatgatggtt cggttttaaa
     2161 caatagttct aattggatat ataaatcaaa gtttttcaaa aaagaatact agtctagatt
     2221 ttgaaataaa tcctaatccc aataatggcc tttttaatgt aaatatactt tgtaagcttg
     2281 actgcgaaat tgattatgaa gtacgagaca ttgcaggacg gctactttta aaatttttct
     2341 ctttggatcc ctcttttgaa attgatatat taaccttttc acccgatgtc tacttattta
     2401 aaatatacac cattaattat agtagctctg aaaaaattat cctaactaat taaacgaaaa
     2461 actatgaaaa gatccatttt aatatacata ctgcttcaca tctgtatcca ttcttactcc
     2521 caatcttgga attgggctaa acatatacat agtggtggag gagaaagagc aacggtttcc
     2581 tgcattgatg ggcaagggaa tagctataca attggagggt ttgatagtaa ctactcatgc
     2641 caatttgata cggttactct ttttggagta ggtacctcaa ctagtttttt agcaaagtat
     2701 gaatctggag gaaatttagt ttgggtgaaa aagattagtt atacctcttc acaaaattgc
     2761 agtggtggaa tagttgattt ggtttatgac tctatctcaa attccttgct ttttactgga
     2821 agtatttgtg gttcttttca aatgaattca acaattatta cttcaagttt cggcagcatg
     2881 gatgctattt ttggaaaagc tgatttgaac ggtgatattt tatggtatga gaccattaca
     2941 ggattaggta ttaaacaagg cagaacaatc gcttacgata atgcgggtag tgtttatgtg
     3001 gcaggaacta atacccatcc aattcaaatt tgaacagata cattgccatt gggtactttt
     3061 ctggtaaagt acactagtag tggtgttcag caatgggcaa aaaatattag tggtcgaggt
     3121 aagtacattg caagtattgc ctttgacccg cgtgatatgc aggtaaaagg caatagtttg
     3181 ttccttaccg gcagtatgtg gcgcgatacc ctgaatatag atacagtaca tattgctatt
     3241 cctaatgcgt atggtacaat tgtttccaaa tttgatttga atggtaacgt tgcctgggcc
     3301 aaagcttttg gatatcctat gggcggagat attggctact cacttgctgt tgattctaat
     3361 tcaaatgtgt atgctatttc tgctttttat ggggattcgg ccatttttgg agatacagtt
     3421 atatttaata gcggtatgcg tgatatgtat tttgtaaagt acaagagcaa tggtgatttt
     3481 agttgggtca ggcaaacgca cagcacattg ggagcagaag gattttctgt agcatctgat
     3541 gcaatggata atttatacat tactggcaaa ttctcaggaa ctgcccaaat tggcaatttt
     3601 caagttgttt ctatgggtgc caatgatatg tttctggcta agtatgcacc caatggagac
     3661 tgcataggtg ttgataattt tgggtatgcg gaggggattt ctgtggtcgt agacaataac
     3721 aacaatccaa ttgtttcagg tatttttaaa aacactgtaa caattggcaa caccagctat
     3781 acctgtaatg atgtatttga tgtttttata gctcaacacg ttgcgattac taatgtaaaa
     3841 aatcaactaa aatcaaacag caaccaactc ctcatccatg ccaacccaaa caccggcaaa
     3901 tgcaacataa gagtgccgga tgaaatgatg catgaaaaaa acttggtact tagtatttac
     3961 aacagccaag gcaaagtaat acagcagata cctgtaaaca caaatgaaga aaaaataaaa
     4021 ataaacttag aagcagaggc gaccggaatt tacaatgcgg tgctcagtaa cggcaacaaa
     4081 acttatagcg gtaaaattgt gtttgaataa gatgtgttaa gtcaacctga ggtaacacga
     4141 agggtggatt agcatttaga attcaattct taaaaccttc gcactgcttc gtttggcctc
     4201 agcatgacat tatttgagaa gtgcaataaa aaagcaagct acttttgata tggaatgtga
     4261 aacacctagc tttgcaacaa gcaaaatatg tatcttactc tttcacgaaa tactgtactt
     4321 tttattggaa tagcaatcct ttcattctac tattgcttaa acaaaattca atttatttta
     4381 gatgcagaaa tcacttatgg aacggtaagt agctccagtg agggaaacaa cagggaaacc
     4441 tcaactattt tctttagcac aaaagatagc aactattttt ttggagctac tgaaaatgta
     4501 cactatacac tcaaccaaac tgttgctgtg atttacaaga gcgaaaaacc gcaacaggct
     4561 tttgtgttta gctttcttgg attttggtac gaaggattac tctattgcct catcccactt
     4621 tgcctgtggt gcgccttcct tctaagcttc tattcatctt ccgattcgtt tattctacct
     4681 ttcttcgcca aaagttcggt aaaacaacaa gcaaaaaatt caacaaaaga tagcgctgtg
     4741 actaagcagt ctccttaact aatcccgcag cagtttgtat atcggcacgg gtatacgcac
     4801 gaatgggaat atttatttct tcgcggtgaa atacttctgg tactttttgg ttattcagca
     4861 ccatatcgag ccatttgtaa taataggccg gagtaattgt atttgctcta acttgtttta
     4921 aaaattcagg ttccaccaaa tccgcttgtt gcaaaaaatc aaaaatcata cgcatgctgt
     4981 actcatccac ctctgcccct ttggctgcgc gagtgggtgc aaaatactcg ttctcttcaa
     5041 ttgctttaaa agtacttttc cctttttcag gataaaactg ataggtattc tttgctgcta
     5101 ttccaaattt ctcggttaag ccaaatgcat gtattgccca tcgtaatgga cctttcaccg
     5161 cttttaaaat cggattggct cgcaaattat ctgtgtttcg tttcttatcc ggtgtccaaa
     5221 taaccaaggt tgctgataat ttttcaggta taaaaacaac atgcggttcc cagctgtcta
     5281 ccaaagaagg attccattcc ttttgatgaa aatgcttgcg aattttcatg ctcacggtta
     5341 agtccttctc atttaaattc aactcctttt caaacaacat actctcatag cctgaaccaa
     5401 aaaaggcatt ggaggtgagt aggtaattgt tgtggtgatg aatgcaatgg gcagcaattc
     5461 cttccaattt tcgctcatgc cgagggaaga ggtgaatttt tatattgatg tgttcgtttt
     5521 cgtacacaaa aaagtaagga atgttataca aggtccaggt ttgtgataaa tacccttcgt
     5581 ccgttaaatt tctccggaca acttccttta aaaattcttt gtcatttccc atttgaacta
     5641 aaatgggcgc tactttttga tgaaaaacat aacggtcgtc ttcggcatct gaaagttgaa
     5701 ttatgctttt tacgtaggga tgttctttta gtacttcgtt cattgttgcg tcaagtgatt
     5761 tcaatggcga aagtaaacaa ttttatgcag cccaaaactc ctactgatga atatcataaa
     5821 acgcaaaagg cacccgaaat tcggatgcct tttacttgat ttatcctgtc ttgtttcaac
     5881 tcaacgagag gaaaccagaa tgaactgata gtattttagt aatagcgcaa acgcaccaca
     5941 cccgacaatt gttttgcaag gcgcattact tggcgtgtgt aaccatattc attgtcgtac
     6001 caaacataga gcactacatt ttttccatct ttagaaacaa ttgtagccgg actatcaaat
     6061 atggaagaac aagggtttcc tactaaatca gaagatacca attcgttgct gaatgaatac
     6121 tgaatttgtt ctaccaaatc tccttttaaa gccgcttctt tcatgatggc atttacttct
     6181 tcttttgcgg cagttttttt gatgttcaaa cttaagatag ccaaagaaac atttggggtg
     6241 ggaacgcgca ctgcatttcc ggttaacttt cctgccaaat gcggcaatac tttcgcaacc
     6301 gctttatcgg cacctgtttc tgtaattacc aaattcaaag ctgcagagcg acctctgcgg
     6361 tattttgcat ggtagttatc caataaattc tggtcattgg tgtaagagtg aatggtttca
     6421 atatgtccgc gttcaattcc taaactctcc tcaattactt ttaacaccgg cacaatggca
     6481 ttggtagtgc aactggcagc ggagaatatg gtttctttgc tgtcgtatga ttcgtggttc
     6541 acaccataca caatattggg aatatcgcct ttgccgggcg cagtaagtaa aacttgcgct
     6601 acgccttttg cttttaagtg cttgcttaag ccttcgcggt cacgcgctac acctgtattg
     6661 tcaatcaaaa gtgcattgtc aataccatac gcagtatagt ctaagtcttc cggattcttt
     6721 ccactcaaca tcaacacact gtgcccgtta attacaattg ctttattctc caaatcttcc
     6781 acaaccgttc cgggaaatgg accatgaact gaatcggttc gtaacaaatc tgctcttttg
     6841 ctgatgtctt catcactgct gctgcgtgta acaatggcac gcaaacgcaa ttgttcacct
     6901 ttaccagctt gtgtaattaa ttcgcgtgca gcgagtcgtc caatgcggcc aaatccatag
     6961 agtataacat ctttgggttt aattgtcttt ttttcagaac ctaaaaaacc ggctagtttg
     7021 tcttcaataa aatgtgtggc attggttccc aacttttttt ccaccaacca ttcagcaccc
     7081 aatcggccaa tatctattcg cgatggagcg atgttgcgca attttgcaat ttcttttgcc
     7141 agcaataagg tgtcttgcac attgatgggt tgctttatga tgttcagcgc atacaggtga
     7201 agattcaaaa tttcgcttgc gctgcgatct accaattggt tgcggaaaat gatgagttcc
     7261 acagatttgt caaaccacaa attaccaatc acattaatta attcaacggc tgctttttcg
     7321 ctttcaatcc aagtagtgag cttattctcg tagtcgttct ttgcttcggc tgttttcatt
     7381 gtttttagtt ttagtgtaga ggattcaaat atgttttgtg attttattca tacgaatgta
     7441 ataaaaaccg cgacaaaatt attcaaattt ttgagatgag aataacccaa atgaaagtgc
     7501 ttgatagctc agccctttga agaaaaccta cacataaaca attacac
//
LOCUS       DAOOOI010000089         7343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1980_length_7343_cov_6.168359, whole genome shotgun sequence.
ACCESSION   DAOOOI010000089 DAOOOI010000000
VERSION     DAOOOI010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1980_length_7343_cov_6.168359"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..86
                     /locus_tag="PKX59_09315"
     CDS             <1..86
                     /locus_tag="PKX59_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosomal protein S19 family protein"
                     /protein_id="HOZ91104.1"
                     /translation="MVGHKLGEFAPTRTFRGHSGGKDKGGK"
     gene            138..566
                     /gene="rplV"
                     /locus_tag="PKX59_09320"
     CDS             138..566
                     /gene="rplV"
                     /locus_tag="PKX59_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOZ91105.1"
                     /translation="MGSRKRLAAEERKEALKNVNFAKLNNCPTSPRKMRLVADLVRGK
                     SVNTALQILRFTNKDAAPRIEKVLLSALSNWQAKNTDQRLEDSDLFIKEIYVDSARMN
                     KRLRPAPQGRGYRIRKRSNHVTLIIDSKVAKTENTNTENN"
     assembly_gap    581..617
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            620..1369
                     /gene="rpsC"
                     /locus_tag="PKX59_09325"
     CDS             620..1369
                     /gene="rpsC"
                     /locus_tag="PKX59_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOZ91106.1"
                     /translation="MRGWDSNWFGGKNYSDKLVEDEKIRNYLMARLSKGGVSKIIIER
                     TLKLITVTINTSRPGIVIGKGGQEVDKLKEELKKLTKKDVQINIFEIKRPELDAKLVS
                     ESVAKQLEGRISFRRAIKTSIASTMRVGAEGIKIMCAGRLGGAEMARTEQYKEGRVPL
                     HTFRADIDYAVSEAQTSYGKIGVKVWICRGEVYGKRDLSPNIGLNTQTKVGSERNEGR
                     GERKERGEGRGSRGTGRSGDNRGRAPKKRDE"
     gene            1416..>1819
                     /gene="rplP"
                     /locus_tag="PKX59_09330"
     CDS             1416..>1819
                     /gene="rplP"
                     /locus_tag="PKX59_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018675405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOZ91107.1"
                     /translation="MLQPKKTKFRKQHKMKAKGNATRGADIAFGSFGLKSLETAWITG
                     RQLEAARIALTRYMKREGQSWIRVFPDKPITKKPAEVRMGKGKGAPEYWVAVIKPGTI
                     MFEAEGVPLNVAKEAMRLASQKLPVLTRFIVRR"
     assembly_gap    1820..1829
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1873..2067
                     /gene="rpmC"
                     /locus_tag="PKX59_09335"
     CDS             1873..2067
                     /gene="rpmC"
                     /locus_tag="PKX59_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOZ91108.1"
                     /translation="MKQNIVKDLTNDELRDKLAEERSAYTKIKITHQVSPLENPMKIR
                     AMRRTVARLKTEMVNRQLSK"
     gene            2076..2330
                     /gene="rpsQ"
                     /locus_tag="PKX59_09340"
     CDS             2076..2330
                     /gene="rpsQ"
                     /locus_tag="PKX59_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOZ91109.1"
                     /translation="MERSLRKEKIGIVTSDKMAKSIVVSIERKEKHPIYGKFIKKTKK
                     FMAHDEKNEAHVGDKVRIMETRPLSKNKNWRLVDVVEKAK"
     gene            2381..2749
                     /gene="rplN"
                     /locus_tag="PKX59_09345"
     CDS             2381..2749
                     /gene="rplN"
                     /locus_tag="PKX59_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOZ91110.1"
                     /translation="MIQQESRLTVADNSGAKEVLCIRVLGGTKKRYASIGDKIVVTVK
                     HAIPSGNVKKGTVSKAVVVRVSKEVRRKDGSYIRFDDNAVVLLNANDELRGTRIFGPV
                     ARELREKEFMKIVSLAPEVL"
     gene            2752..3087
                     /gene="rplX"
                     /locus_tag="PKX59_09350"
     CDS             2752..3087
                     /gene="rplX"
                     /locus_tag="PKX59_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOZ91111.1"
                     /translation="MEKKVKLKIRKGDMVIIMSGESKGKQGKVLSVVKESNRAIVEGV
                     NQVSKHSKPNAKNTQGGIVKQEASIHMSNMMLVDPKTGKPTRVGRKVEDGKIVRYAKK
                     SGDLIKENK"
     gene            3139..3696
                     /gene="rplE"
                     /locus_tag="PKX59_09355"
     CDS             3139..3696
                     /gene="rplE"
                     /locus_tag="PKX59_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOZ91112.1"
                     /translation="MAYSPRLKDKYKNEIAKSMMTRFSYKSNMQVPRLVKICVNQGVG
                     DAVSDKKLIESSVNEMSTITGQKAVATKSKKDISNFKLRKDVPVGVRVTLRDNNMYEF
                     LDRLVAVSLPRIRDFKGINDKGFDGRGNYTLGVTEQIIFPEIDIDKVNKIQGMDITFV
                     TTAKTDTEALALLKEFGLPFKNQKQ"
     gene            3703..3972
                     /gene="rpsN"
                     /locus_tag="PKX59_09360"
     CDS             3703..3972
                     /gene="rpsN"
                     /locus_tag="PKX59_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOZ91113.1"
                     /translation="MAKESMKAREVKREKLVAKYAAKREALKKAGDWAGLQALPKNSS
                     PVRLHNRCKLTGRPRGYMRQFGISRVLFRSMALEGKIAGVTKSSW"
     gene            4039..4437
                     /gene="rpsH"
                     /locus_tag="PKX59_09365"
     CDS             4039..4437
                     /gene="rpsH"
                     /locus_tag="PKX59_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOZ91114.1"
                     /translation="MTDPISDFLTRLRNAIKANHRIVEIPASNLKREITKILFDKGYI
                     ANYKFEETENKQGSIKIALKYNAITKEAAIRSLVRISTPGLRKYANHETLPRVLNGLG
                     VAIISTSKGVMTDKEARLMNVGGEVICYIY"
     gene            4459..5013
                     /gene="rplF"
                     /locus_tag="PKX59_09370"
     CDS             4459..5013
                     /gene="rplF"
                     /locus_tag="PKX59_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOZ91115.1"
                     /translation="MSRVGKLPISLPKGVTVSVNGTEVSVKGPKGTATQNIADGIDVA
                     IDGNVVTVSRATEQKRHKAMHGLYRALIANMIKGCSEGYKIEQELVGVGYRASNQGNL
                     LDLVLGFSHHVVFELPSEIKVSTRQDRGQNPIITLESHDKQLIGAVAAKIRSLRAPEP
                     YKGKGVKFVGETLRRKAGKSAAKK"
     gene            5068..5415
                     /gene="rplR"
                     /locus_tag="PKX59_09375"
     CDS             5068..5415
                     /gene="rplR"
                     /locus_tag="PKX59_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOZ91116.1"
                     /translation="MQAKKNIRRSSIRKRIRKTISGSSSRPRLSVFRSNKAIYVQLID
                     DVNGHTMAAASSQSISEKMTKIEKSIRVGQLIAEKAKAVGVEEVVFDRGGYLYHGRVK
                     SLAEGARSAGLKF"
     gene            5420..5941
                     /gene="rpsE"
                     /locus_tag="PKX59_09380"
     CDS             5420..5941
                     /gene="rpsE"
                     /locus_tag="PKX59_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOZ91117.1"
                     /translation="MMLTKAKRVKSTDTELKDKLVGVQRVTKVTKGGRAFTFSAIIVV
                     GDEKGVVGYGLGKAKEVTDAVTKGIEDAKKNLIKVKVLKGTVPHEQEGKFGGARVFLK
                     PAAAGTGVIAGGAMRAVLESVGVTDVLAKSKGSSNPHNVVKATIDALAQMRDAIEVSQ
                     ARGVKMARVFNGN"
     gene            5962..6141
                     /gene="rpmD"
                     /locus_tag="PKX59_09385"
     CDS             5962..6141
                     /gene="rpmD"
                     /locus_tag="PKX59_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOZ91118.1"
                     /translation="MSKVKITQIKSGIDRPLRQKKTLQALGFRKLNQSIELENTPQIA
                     GMIRQVNHLLKVENI"
     gene            6161..6607
                     /gene="rplO"
                     /locus_tag="PKX59_09390"
     CDS             6161..6607
                     /gene="rplO"
                     /locus_tag="PKX59_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOZ91119.1"
                     /translation="MNLNTLKPAAGSVRSNNRNGRGQGSGGGGTARRGHKGAQSRSGY
                     SAKVGFEGGQMPLQRRVPKFGFKNINRVEYKAINLDAVQQLIDKHNITDITLEVLIEK
                     GLVAKRVLVKILGRGEIKAKANITAHAFSATAKQAIESLGGTASTI"
     gene            6615..>7343
                     /locus_tag="PKX59_09395"
     CDS             6615..>7343
                     /locus_tag="PKX59_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOZ91120.1"
                     /translation="MKKLITTLQNIWKIEDLKSKITVTLICILVYRFGAHIVLPGVDP
                     QALGALKSQTEGGILGILDMFAGGAFSKASIFALGIMPYISASIVVQLLSMAVPRFQK
                     LQKEGESGRKKLSQYTRFLTVLVCLGQAPGYLVNLQSQAPGAIIATTHPELVSMFQFT
                     LTSVLIITAGTLFVMWLGERIQEKGIGNGTSLIIMVGIIARMPHAIYAEFVSRLGDTG
                     GAVVFLIEILIWLLVIAAVILLVQG"
BASE COUNT         2651 a         1152 c         1593 g         1900 t
ORIGIN      
        1 atatggtagg tcacaagcta ggcgaatttg cgccaaccag gactttccgt gggcatagtg
       61 gtgggaaaga taagggcggc aaataaattg agccgttgaa ataaaattaa gctaatactc
      121 taaataataa aaagaaaatg ggttcaagga aaaggttagc agccgaagaa cgcaaagaag
      181 ctttgaagaa tgtaaatttt gcaaagctga acaattgccc aacctcgcca cgtaagatgc
      241 gtttggttgc tgatttggta cgtggtaaaa gtgtaaatac agcattacaa attttacgat
      301 ttactaataa ggatgcagcg ccccgtatag aaaaagtttt attgtcagct ttatctaatt
      361 ggcaggcaaa aaatacagac caacgattag aagacagtga tttgtttatc aaagaaattt
      421 atgtggacag cgcccgtatg aataaacgat tacgccctgc accacaaggt cgtggatacc
      481 gtatccgtaa acgcagtaat catgttacct tgattattga ctctaaagta gctaaaaccg
      541 aaaacactaa tacagaaaac aattaaaatg ggacaaaaag nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnntta taagaggctg ggattcaaac tggtttggtg gaaagaacta
      661 ttcagataaa ctggtagaag atgaaaagat acgcaactac cttatggctc gtttgtcaaa
      721 aggtggtgtt tcaaaaataa ttattgaacg tactttaaaa ttaataacgg ttactataaa
      781 tacatcacgt ccgggcattg taattggcaa gggcggacag gaagttgaca agctaaaaga
      841 agaattaaaa aagcttacca agaaggacgt tcaaataaac atttttgaaa taaaacgtcc
      901 tgagttagat gccaagttgg ttagtgaatc ggtggctaaa caattagaag gtcgcatatc
      961 gtttcgtaga gctataaaaa cctctattgc ctcaacaatg cgtgtaggtg ccgaagggat
     1021 taagattatg tgtgcaggca ggttaggtgg tgctgaaatg gcgcgtacgg aacaatacaa
     1081 agaaggtcgt gtgcctttgc acacattcag agctgatatt gattatgcgg tatctgaagc
     1141 acaaaccagc tatgggaaaa ttggtgttaa agtatggatt tgtcgtggcg aagtttatgg
     1201 caagcgtgat ttatctccaa atatcggatt aaatacacaa acgaaagtag gtagcgaacg
     1261 taacgaaggt cgtggagagc gtaaagagcg tggcgaaggc cgcggaagca gaggtactgg
     1321 gcgcagtggt gataacagag gacgcgcacc aaaaaagaga gatgaataaa acggatcaag
     1381 taaaataata attagaaatt taaaaatatt aataaatgtt acagccaaag aagactaaat
     1441 tcagaaagca gcataagatg aaagcaaagg gtaatgctac ccgtggtgct gatattgctt
     1501 tcggatcttt tggtttgaag tcattagaaa cggcatggat tacaggccgc cagttagaag
     1561 cagctcgtat tgcacttacc cgatatatga aacgtgaagg ccagtcttgg atccgcgttt
     1621 ttcctgacaa gcctatcact aaaaaacctg cagaggtacg tatgggtaaa ggtaaaggtg
     1681 caccagagta ttgggttgca gttattaaac cgggtaccat tatgtttgaa gcagaaggcg
     1741 ttccattaaa tgtagctaaa gaagcaatgc gccttgccag ccaaaaacta ccggtattaa
     1801 ccagatttat tgtaagacgn nnnnnnnnnt agagtaagcg ttaaaaagta aaatttataa
     1861 aaaagattta caatgaaaca gaatatagta aaagatttga cgaatgacga actgcgcgat
     1921 aagttagcag aagaacgttc ggcttataca aaaattaaaa taacacatca ggtttctcca
     1981 ttagaaaacc cgatgaaaat acgtgccatg cgcagaacag tagcacgctt aaaaacagaa
     2041 atggttaacc gtcaattatc aaaataaatt tacacatgga aaggtcactt cgaaaagaaa
     2101 aaatcggtat cgtaacgagc gataaaatgg ctaaatcaat tgtggtatca attgaacgca
     2161 aagaaaagca tccgatttat ggtaagttta ttaaaaaaac caaaaaattt atggcgcatg
     2221 acgaaaaaaa tgaagcgcat gtaggtgata aggtacgcat tatggaaaca cgcccattaa
     2281 gcaaaaacaa gaattggcgt ttggttgatg ttgtagaaaa ggcaaaataa tcgaacctat
     2341 taaagaaatt atttttttga aaaaatatca agaataaaaa atgatacaac aagagtcgag
     2401 gctaaccgta gccgataata gtggagcaaa agaagtgcta tgtattcgtg tattgggtgg
     2461 taccaaaaag cgatatgcta gcataggcga taaaattgta gttaccgtaa aacatgcaat
     2521 tccatccggt aatgttaaaa aaggtaccgt ttcaaaagct gttgtagtac gtgtaagtaa
     2581 ggaagtgaga cgaaaagacg gctcatacat caggtttgac gacaatgcag ttgtgttatt
     2641 aaatgcaaat gatgaattac gtggtacacg tatttttggc ccagttgcac gagaattgcg
     2701 cgaaaaagaa tttatgaaaa tagtttcatt agcaccggag gtactataat tatggagaag
     2761 aaagtaaaac taaaaataag aaaaggcgac atggtgatta ttatgtcggg cgaatcaaaa
     2821 ggtaaacaag gtaaagtatt atctgttgta aaagaatcaa accgggcaat tgttgaaggc
     2881 gttaaccagg tttcgaaaca tagcaaacct aatgctaaaa atacccaagg tggtattgta
     2941 aagcaagagg ccagtatcca tatgagcaac atgatgttgg tagaccccaa aaccggtaaa
     3001 ccaacacgtg taggccgtaa agttgaagat ggaaaaattg tgcgttacgc aaaaaaatca
     3061 ggcgatctga taaaggaaaa caagtaataa gaaaaagttt tagttgctac atgtaacaga
     3121 cttaaaaata ttaataacat ggcttacagt ccacgattaa aagataaata taaaaacgag
     3181 atagcaaaaa gcatgatgac acgctttagc tacaaaagca atatgcaggt gcctcgttta
     3241 gtgaagatat gtgttaacca aggtgttggt gatgcagtat cagacaaaaa attgatagaa
     3301 tcatcggtta atgagatgtc aactataacc ggccaaaaag cagttgctac caaatctaaa
     3361 aaagatattt ccaattttaa attgagaaaa gatgtacctg taggtgttcg tgtaacttta
     3421 cgcgacaaca atatgtatga atttttagac cgtttggttg cagtatcttt accccgcatt
     3481 cgcgatttca aaggtattaa tgataaaggt tttgatggtc gtggaaatta cactttgggt
     3541 gttacagagc aaattatatt tcctgaaatt gatattgata aggtaaataa aattcaaggt
     3601 atggatataa catttgtaac aacagcaaaa acagataccg aagcattagc attactaaaa
     3661 gaatttggtc ttccatttaa aaatcagaaa caataatatt ccatggctaa agaatcaatg
     3721 aaagcgcgcg aagtgaagcg cgaaaaatta gttgcaaagt atgctgccaa acgcgaagct
     3781 ttaaaaaaag caggagattg ggcaggttta caagcactac ctaaaaattc ttccccggta
     3841 cgtttgcaca atcgctgtaa gttaactggc aggccacgag gctacatgcg tcaatttggt
     3901 atatcacgtg tgttattcag gtcaatggca ttagaaggta aaattgccgg agtaacaaaa
     3961 tcatcttggt aaaataaaca acatattacg ggtagtgtgg atatcgcccc gggtaaattc
     4021 aaataaaaat taaaatcaat gacagatcca atatcagatt tcttaaccag gttaagaaat
     4081 gcaattaaag ctaaccaccg tattgtcgaa attccggcct caaatttgaa gcgtgaaatc
     4141 acaaaaatac tattcgacaa aggatatatt gcaaattata agttcgaaga aacggagaat
     4201 aagcaaggtt caattaaaat agcactaaag tataatgcaa taaccaaaga agctgcaatc
     4261 agaagtttgg tacgcattag tacacctggc ttacgtaagt atgccaacca tgaaacttta
     4321 ccacgtgttt taaatggttt aggtgttgct atcatatcta cttcaaaagg tgttatgact
     4381 gataaggaag cacgtttgat gaatgttggt ggcgaggtaa tttgttatat ttattaatcc
     4441 taaaaaatta gaaagacgat gtcacgcgtt ggaaaactcc ccatatcatt accaaaaggt
     4501 gtaacagttt ctgttaatgg aaccgaagtt tcggtaaaag gtcctaaagg tactgctaca
     4561 caaaatattg ctgacggaat tgatgttgct attgatggca atgttgttac tgtaagccgt
     4621 gcaactgaac aaaaaagaca taaagctatg catggtttat atcgtgcatt aatagctaat
     4681 atgattaaag gctgctctga aggatataaa attgaacaag aattagtagg tgttggttac
     4741 cgtgcttcaa accaaggtaa tttgttagac ctggtactag gtttttctca ccacgtagtg
     4801 ttcgaacttc cttctgaaat taaggtttca accaggcaag accgtggtca gaatccgatt
     4861 attaccttag aaagtcatga caaacaactt attggtgcag tagcagcaaa aatccgctca
     4921 ttacgtgcgc ccgagcctta caaaggaaaa ggtgttaaat ttgttggtga aactttaaga
     4981 cgtaaagcag gtaaatcagc agctaaaaaa taacaagtaa aatgatgttt taacaagtta
     5041 ctatgaaaca tcgcaacaaa acataaaatg caagcaaaaa agaacataag aagaagtagc
     5101 attcgaaaac gaattcgtaa aacgatttct ggtagttcaa gtcgtccaag gttgtcggtt
     5161 ttcagaagca acaaagctat atacgtacaa ctaattgatg atgtaaacgg ccatacaatg
     5221 gcagcagcgt cttcacaaag cataagcgaa aagatgacta agattgaaaa atcaataaga
     5281 gtaggtcaac ttattgctga gaaagcaaaa gctgttgggg tagaagaagt agttttcgac
     5341 cgtggtggtt acttatacca tggcagggtt aaatcattag cagaaggtgc acgtagtgcc
     5401 ggattaaaat tctaaaatta tcatgttaac aaaagcaaaa agagttaaat caaccgacac
     5461 tgaacttaaa gataagttag taggcgtaca acgtgttacc aaggtaacca aagggggtag
     5521 agcttttaca ttttcagcaa taattgtggt tggtgatgaa aaaggtgttg ttggttatgg
     5581 tttaggcaaa gccaaagaag taaccgatgc ggttaccaaa ggaattgaag atgctaaaaa
     5641 gaatctgata aaagttaaag tactaaaggg aaccgttcca catgagcaag aaggtaaatt
     5701 tggcggtgca cgtgtcttct taaagccagc cgctgcaggt accggtgtaa tagccggtgg
     5761 tgccatgcgt gcagttttag aaagtgttgg tgtaaccgat gtattggcaa aatcaaaagg
     5821 tagttcaaat ccccataatg tggtaaaagc aactattgat gctttagcac aaatgcgcga
     5881 tgctatagaa gtatcacaag cacgtggtgt taagatggca cgtgtattta atggaaatta
     5941 attttacaaa cgaaagcaat catgagtaaa gtaaaaataa cacaaataaa gagcggtatt
     6001 gacaggcctt tgcgtcagaa gaaaactttg caagcattgg gatttcgcaa actaaatcaa
     6061 tctatagagt tagaaaacac cccacaaata gcaggtatga tacgtcaagt aaatcatttg
     6121 cttaaagttg agaatatcta acccataaaa aactaataca atgaatttaa atacattaaa
     6181 gccagccgca ggatcggtaa gaagtaataa cagaaatggc cgtggacaag gttcaggtgg
     6241 tggtggaact gctcgcagag gtcataaagg tgcgcaatcg cgctcaggct attcggcaaa
     6301 agttggtttt gaaggtggtc aaatgcccct gcaaagaagg gtgcctaaat ttggtttcaa
     6361 aaacattaac cgagttgaat ataaggccat aaacctagat gctgtgcaac aattaattga
     6421 taagcacaat atcactgata ttaccttaga agtattaata gaaaaaggat tagtggcgaa
     6481 acgtgttttg gttaaaatat taggtcgcgg tgagattaaa gccaaagcca atattacagc
     6541 acatgcattt tcggctacag ctaaacaagc aatcgaatca ttgggtggta cggcctcaac
     6601 tatctaaaaa ataaatgaag aaactcataa ctacattaca gaatatctgg aagattgaag
     6661 acctgaagtc gaaaatcaca gtaacactta tttgtatact ggtttatcga tttggagctc
     6721 atattgtatt gccaggtgtt gacccacaag ctttaggcgc tttaaagagc caaaccgaag
     6781 ggggaatatt aggcatctta gacatgtttg ctggtggtgc ttttagtaag gcatctattt
     6841 ttgcattggg aattatgcct tacatatcag ccagtattgt agtacaatta cttagtatgg
     6901 ctgtacctcg ttttcaaaag cttcaaaaag aaggcgaaag cggacgcaaa aagttaagcc
     6961 aatacactag atttttaacc gttttagttt gtttaggcca agccccggga tatttagtaa
     7021 atctgcaatc gcaggctccg ggcgccatta ttgccacaac acatccggag ttggtaagta
     7081 tgtttcagtt tacgcttact tcagtattaa ttattacagc aggaacatta tttgttatgt
     7141 ggttgggcga acgaattcaa gaaaaaggaa ttggaaacgg tacctcatta attattatgg
     7201 ttggtattat tgcgcgtatg ccacatgcaa tttatgctga gtttgtatca cgtttaggag
     7261 atactggtgg tgctgtagta tttttaatag aaatattaat atggttattg gtaatagcag
     7321 ccgttatact tttagtacaa ggt
//
LOCUS       DAOOOI010000090         7341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1981_length_7341_cov_7.618446, whole genome shotgun sequence.
ACCESSION   DAOOOI010000090 DAOOOI010000000
VERSION     DAOOOI010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7341
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1981_length_7341_cov_7.618446"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..>977
                     /locus_tag="PKX59_09400"
     CDS             140..>977
                     /locus_tag="PKX59_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HOZ91121.1"
                     /translation="MNHGVLYALLLDSNIAGIKWAVPSLIFVLITIQAFLFNALINRF
                     EVLYKSSNIPVIIFIILYGLIPGFLALSPFLLVKTLMPVALWKIFTLYKSERPVTRAF
                     DTGLVFSLMILCYLPMALLVVFMYLCIAILRPFNWREWVASALGLLTPAILLFTILFV
                     ADKLHLRNTEVNTDSWLPVIRLQSLSWRFILPALVMAILLFLSFLKLYANYSKNVIRM
                     RKFQLCFMLLLIFLLLIILIPVQQSASRFILLIPPVCLFLSYYFIAIRKVWWFELLSI
                     VLV"
     assembly_gap    978..1017
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1430..>4570
                     /locus_tag="PKX59_09405"
     CDS             1430..>4570
                     /locus_tag="PKX59_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOZ91122.1"
                     /translation="MLWTTSAQAQLAIKYPLTISSGTYSSISGSGAPVAIIGDDMAVN
                     ITGLTGFTVNGTTYTNARMCSNGWLALYGASVPTTTGTYAPLSTAITNGAVIFAPFGR
                     DLNSQSGTGAYWQTIGNELIFEWVNYIRYAQTGESLNFQIRLNTVTGEIKFVYGTMTA
                     LTGSTYPQVGWKTNGTVASNWATDVNNLMIDNVGSPNTCNWSNAVTGNANTSTMYFNS
                     ANATIKPNSGLTYTWTKPVATDPEPVRTHAAVSGITTNNATLNWTAPANATQYNVQYR
                     TVGSCSWTNWSGNPVGTNSVTLTGLTSSTTYQVRVQASNGSTNAIYSHIPNLAGTGNG
                     YVAAGTFTTVASCVAPTAPVGSPTTATTANISWTAASPVPSNGYEYAVTTSAVPPVSG
                     TAFAGTSTSVSGLTPATTYYLHVRSYCGGSDYSAWVTSASFNTPCNAIATLPWTEGFE
                     SAVIPAYPSTCWYEDPSSGDWITTNNSSSSNDADARTGAQFLRDAYSATNEFIWTPGF
                     SLTAGQSVDFSFWWAGDNYSGWTGDVFYNTAQTGTGATQMGTSFVTSGTTTTKTYAKQ
                     TRTLVAPATGTYYFAIRVNATGGPWYLSFDDFNVENTPSCVAPTALVGTPTTATTANI
                     SWTAASPVPSNGYEYAVTTSATPPGSGTAFAGTSTSVSGLTPSTTYYLHVRSYCGGSS
                     YSTWASSASFMTGQIPATLPYSNSFGSNDFAFVNGSQTNQWAYGSATGNPANAIYVSN
                     DNGTSNAYTISSLSVVQAFRDIAIPNGTTLTNFSFNWIANGESCCDYLRVWLVPTSYN
                     PTAGTQITAGAGRIQIGSNMNVQSAWQTYTNNTLDLSSFAGQTMRLVFEWKNDGSIGT
                     QPPAAIDNISLAQVASCSGTPAPGNTQSNLPSACSAQSFTLSMSNTINDIGITYDWQS
                     SPDGITWTSTGGTNATYTTTQTAATYYQCVVTCTNSGMSATSTPVQVTMSPFYNCYCT
                     TNLHTSSSPCITQVDFNTISNNTAGAGCALPAYSYQAATTTVLKGSSYTFTRTTTSTG
                     VWTGVWIDYDQSGTFDA"
     assembly_gap    4571..4592
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4657..6195
                     /locus_tag="PKX59_09410"
                     /pseudo
     CDS             4657..6195
                     /locus_tag="PKX59_09410"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
     gene            complement(6480..7187)
                     /locus_tag="PKX59_09415"
     CDS             complement(6480..7187)
                     /locus_tag="PKX59_09415"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91123.1"
                     /translation="MNNLPTGMSTIYVADGNACQAQISGSLTYTSTAPPAQSVNVPIL
                     GMPAYACNGTTATISVAAVANATQYTWDGPVGTTFNGNPSPYISTTPSVTVVFGAPNG
                     SGYRIGVQAGNGCGSSLRKSQWLRGIVSTPAAVSGATTTCANSSGTYSTVAVDGATSY
                     LWTVSGDATVSGNGTSVTVNFGPSWTGGTLCVSAQTPCFNSPSKCLVIGVGDGDKLND
                     HTVHELPPPSICGTTIQ"
BASE COUNT         2192 a         1433 c         1425 g         2229 t
ORIGIN      
        1 cacaaagttg ctgaaaaaaa tgttgatcgt caggttgttt aaatcttcgc aaacaccacc
       61 actgttttta gtgccggtga taacactggc attagtacta ttcaacattt atactcaagg
      121 ggttgtagtt atcagagaca tgaatcatgg tgtgttgtat gccttattac ttgacagtaa
      181 cattgcagga ataaaatggg ctgtaccttc attgatcttt gtactcatta ccattcaggc
      241 ttttttattt aatgcactca tcaaccggtt tgaggtttta tacaaaagca gcaacattcc
      301 tgtcattata ttcatcatcc tctatggact tatacccggt ttcttggcac ttagtccttt
      361 tcttttagta aagacattga tgcctgttgc gctatggaaa atttttacat tatataaatc
      421 agagcgtcct gttacccgtg catttgatac aggtcttgtt ttttcgctga tgatattgtg
      481 ttatctacca atggcattgt tggtagtctt tatgtactta tgtattgcca tactacgtcc
      541 ttttaactgg cgcgagtggg ttgcttctgc attaggactt ttaactcctg caatactgtt
      601 gttcacaatt ctttttgtgg cagacaaact gcatttgcga aatacagaag taaatacaga
      661 ttcatggctt cctgttatca gactgcaatc gctttcatgg cgttttatac ttcctgcatt
      721 agtaatggca atccttttgt ttttatcgtt tctgaaacta tatgctaatt acagtaagaa
      781 tgttatccgc atgcggaaat ttcagttatg ctttatgtta ttgcttattt ttctgttgct
      841 cataatattg attcctgtac aacaaagtgc atcacgtttc attctactta tcccacctgt
      901 ttgtctgttt ttgagttact actttattgc aattcgaaaa gtatggtggt tcgaattact
      961 gtctattgta cttgttgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg
     1021 ggttactgga atctttaagt ttttcatttt aattgaagaa tgattacaat cattttgaag
     1081 taatattttc agtttttaaa atataattta ttgaaaatga tgtgataatg ttttaatgat
     1141 tgtcatacta gtatcaaaat accagcaaag gcttgtttaa ttaaaactta atgaataata
     1201 ttgaggccga tttcaaattc cgaattagta tttagatgct tttctacgag gcctcttggt
     1261 cttttttaat gcgtttttaa ctggcgggct ggtgaatgaa gaatagaata gtcgcaaaaa
     1321 atttttaata aataaatgtt taactaaaat caaatttatc atggtagaaa aaaccaaaaa
     1381 tcgaacacga acgtgtttat tctttctatg gtgttgcata atgtctgcaa tgctatggac
     1441 aacaagcgca caggcgcaat tagcaattaa gtatccgtta accattagct caggaacata
     1501 tagttctatt agtggctctg gtgctcctgt tgctattatt ggtgatgata tggcagtaaa
     1561 tattacagga ctaacagggt ttactgtaaa tggtactact tacaccaatg cacgaatgtg
     1621 ctccaatggc tggttggcat tatatggtgc cagcgtacca actacaactg gaacatatgc
     1681 accattaagt acagcaataa caaatggagc tgtaatattt gctccttttg gtagagattt
     1741 gaattcacag agcggaacag gagcttattg gcaaaccatt ggaaatgagc tgatttttga
     1801 atgggttaat tacattcgtt acgctcaaac tggagagtct ttaaactttc aaatacgttt
     1861 gaatacggta acaggagaaa taaagtttgt ttacggtaca atgactgctt taacaggcag
     1921 tacatatcct caagttggtt ggaaaacaaa tggtacagtt gcatctaact gggcaacaga
     1981 tgttaataat ttaatgattg ataacgttgg ttcgccaaat acttgcaact ggagtaatgc
     2041 tgtaacagga aatgctaata ccagtacaat gtattttaac agtgcaaatg ctaccattaa
     2101 gcctaatagc ggtcttactt atacatggac aaaacctgta gcaacagacc ctgagcctgt
     2161 acgtacacat gctgcagtta gcggcataac aaccaataat gcaacattaa actggacagc
     2221 accggctaat gctacgcaat acaatgttca atatcgtaca gttggttcat gttcatggac
     2281 aaactggagt ggtaatcctg ttggaacaaa tagtgtaaca ttaacaggct taacatcttc
     2341 aactacatac caagttaggg tgcaagcttc aaatggttca actaatgcta tttattctca
     2401 tattcctaat ctagcaggca ccggcaatgg ttatgttgca gcaggtacat ttactacagt
     2461 cgcatcttgt gttgcaccaa ctgcaccggt tggttcacca actacagcaa ctacagccaa
     2521 catcagttgg acagcagcaa gtcctgtacc ctcaaacggt tatgaatatg ctgttacaac
     2581 aagtgctgtg cctcctgttt caggtactgc ttttgccggt acttcaacaa gtgttagtgg
     2641 cttaactccg gctaccactt attatctgca tgttagaagt tattgcggtg gtagtgatta
     2701 cagtgcatgg gtaacgagtg cttctttcaa tacgccatgt aatgctattg caacactacc
     2761 ttggacagag ggttttgaaa gtgccgttat tccggcatat ccaagtactt gctggtatga
     2821 agatccttct tccggtgatt ggataactac aaataatagt agctcttcaa acgatgctga
     2881 tgcccgcaca ggcgctcagt ttttaagaga tgcttatagt gcaaccaacg aatttatctg
     2941 gactcccgga ttttcattaa cagcaggcca aagtgttgat ttttctttct ggtgggcagg
     3001 tgataactac agtggatgga caggtgatgt gttttacaat actgcacaaa caggaacagg
     3061 agccacacaa atgggtactt catttgttac ttcaggtaca acaacgacaa aaacttatgc
     3121 aaaacaaaca agaactttag ttgcaccagc aacaggaact tattattttg caataagagt
     3181 aaatgcaaca ggtggcccat ggtatttgag ttttgatgat tttaatgtag aaaatacacc
     3241 gagttgtgtt gctccaacag cattagttgg tacgccaaca acagcaacaa ctgctaacat
     3301 cagttggact gcggcaagcc ctgtaccatc aaacggttat gaatatgccg ttacaacaag
     3361 tgccacacct cccggctcag gtactgcatt tgcaggtaca tcaacaagcg tgagtggttt
     3421 aacaccatct acaacttatt atcttcatgt aagaagttat tgtggtggta gtagttatag
     3481 tacttgggct tcaagtgctt catttatgac aggtcaaata cctgctacat tgccatatag
     3541 caatagtttt ggaagtaatg attttgcctt tgtaaatggc tcacaaacca atcaatgggc
     3601 ttatggctcg gctactggaa atcctgcaaa tgcaatttat gtttctaatg ataatggtac
     3661 aagtaatgca tacacaattt caagtttaag tgtagtacaa gctttcagag atattgctat
     3721 acctaacggc actaccttga caaactttag ctttaattgg atagccaatg gagaatcttg
     3781 ttgtgactat ttaagggttt ggttagtacc aacatcctat aatccaacag caggtactca
     3841 aataacagca ggagccggca gaattcagat aggatcaaac atgaatgttc aaagtgcatg
     3901 gcaaacctat accaataata cattagacct tagctccttt gcaggtcaaa ctatgcgttt
     3961 ggtttttgaa tggaaaaatg atggaagtat tggtacacaa ccaccggcag caattgataa
     4021 tatttctctt gcacaggttg cctcatgcag tggtacacct gcacccggaa atacacagtc
     4081 aaatttacca tctgcttgta gtgcccaatc atttacatta tcaatgtcaa acactattaa
     4141 tgatattgga attacttatg attggcaatc gtcaccagac ggtataacat ggacaagcac
     4201 cggaggaacc aatgctactt acacaacaac acaaactgct gcaacatact atcagtgtgt
     4261 agttacttgt acaaatagtg gaatgtcagc tacttcaaca cctgttcagg ttacaatgag
     4321 tccattttat aattgctatt gtactacaaa ccttcatacc tcatcgagtc catgtattac
     4381 tcaggttgat tttaatacta tcagcaataa tacagccggg gcaggatgtg ctttacctgc
     4441 ttattcttat caggcagcaa caacaactgt tttaaaaggt tcatcatata cttttaccag
     4501 aacaacaact tcaacagggg tgtggacagg tgtttggatt gattatgacc aaagcggaac
     4561 ttttgatgcc nnnnnnnnnn nnnnnnnnnn nnatatacag aggtttcaag tacttcaaca
     4621 ggagcattaa caaatagtgt tacaatcact attccggtga ctgcattaga tggaaatacc
     4681 ggaatgcgta tccgtcaacg tacagttgac atgttggcta ctgatgcttg tacccaaggt
     4741 tttggttcag gtgagacaga agattataca gttgatattc aaacaccggt aggttgctca
     4801 ggaacaccat ctgcaactat tggtggtgcc cctgctaatg tttgtgcagg cgttcctttt
     4861 acacttactg caggtccaac aggtttagca ggatttactt atcagtttca gaactctact
     4921 gatggtggtt taacctggaa taatatggga attgctgatg tttttaataa ctacattgta
     4981 agcaaccaaa cagtaacaac catgtatcag gttattgtta cctgtacaaa cagcggttta
     5041 tctaatacat caactgctgt aacagtaaca caaaattcac caacagcatg ttattgtaca
     5101 ccaactacag ttaatggatt tacgtattac atcaccaact ttaccacaac cggtggtatt
     5161 accaacatta acaatagcag tgtaggtgga ggcccctatc agaatttcag tggtagtgca
     5221 tctgcatcag caaatccggg tgctacaata aattatagta tctctgttac aagtggtact
     5281 tatggacgtg ccatttggat agacttgaac gaagatggtg ttttccaagc aacggaacaa
     5341 ttagtaacca gttcaagttt tctttcatca cctttaacag gtagcttcat tatacctaat
     5401 ggaacaacag caggtactaa acgtatgcgt gtattggcta gctggtctta cagtggtcca
     5461 acagatccat gtaatatcag tggtaatggt gagtatgaag attacagttt tgaagttgta
     5521 gcacttccat catgtacagg cacacccgca gcaacaatca gtggtccggc aaatatttgt
     5581 gcaggcattg gttttaattt aacagcaggt ccaacaggcg ttgatggttt aacttatcag
     5641 tttcagaatt caacagatgg cggaataaca tggaataatc ttggttcagc aggcattaat
     5701 aatatctatg ctataaccag ccaatcggtt actacgcagt atcaggtcat agtttactgc
     5761 tcttacagtg gattaaacgc aacttctact ccaattacgg ttactcaaaa tttaccttct
     5821 gcatgttatt gtacaccaac aaccggtggt ggtttaactt actacatcag taactttact
     5881 acaaccggtg gaactactaa tattaatcaa agtagtggtg gcagttcaac aggttatgat
     5941 gatcatagcg caacagtttc aggtgaattt gaaccaggct caactatcaa ttttacaatg
     6001 acaatagccg gatcaagtaa ctttggtcac gctatttgga ttgattttaa tgccgatggt
     6061 acttttcagg caacagaaca gattgttaca tctaatagtt atctgtactc accaattaca
     6121 ggcagcttta ctattccatt aaatgcaact acaggagtta gacgcatgcg tattttaaca
     6181 agttttacac ctagtaatcc aacaaaccca tgtaccaaca gcggacaagg tgagtacgaa
     6241 gattacagca taaacattac acctcctgta tgtatatcag cgccatcatc acctgcaaat
     6301 gcaggcagtg catgtgctga tgtaattacc aatactgttc tgttaagctg gccttcattg
     6361 tccggtgcta caggatatga tgtgtatttc gatgccaaca atcctccaac aacattagta
     6421 tcagcaaatc aggggggatt atctttcagc gcagatgtat caggtggagc acctgtatat
     6481 tactggatgg tagtgccaca gatacttggt ggtggcagtt catgtactgt atggtcattt
     6541 agtttatcac catcacctac gccaattaca agacacttcg atggtgaatt gaaacatggt
     6601 gtctgcgcac ttacacaaag agtaccacct gtccatgaag gaccaaagtt tactgttaca
     6661 cttgtgccat taccacttac agttgcatcg cctgaaactg tccatagata ggatgttgct
     6721 ccgtcaacag caactgtact gtacgtacct gagctgtttg cacatgttgt agttgctccg
     6781 cttactgctg caggagtact tactatacca cgcaaccact gactcttacg taatgagcta
     6841 ccacaaccgt taccggcctg aacacctatg cggtatcctg atccatttgg tgctccaaac
     6901 acaacagtta ctgatggtgt agttgaaata tagggtgatg gattaccatt gaatgttgtt
     6961 ccaacagggc catcccatgt atattgcgtt gcattagcca ctgccgcaac acttatggtt
     7021 gctgtggtac cattacaagc ataagcaggc attcctaaaa ttggaacatt tactgactgt
     7081 gcaggaggtg cagtactggt ataagttaat gaacctgaaa tctgcgcctg acaagcattg
     7141 ccatctgcca cataaatagt gctcattcct gtcggaagat tattcatgat tccatctaca
     7201 ttaaagttta taaaatcaga agtaaaacta tatggagata aaccgcccga agctgatacg
     7261 taaataatac catcactgcc accaatacat caagattgtt tgaaataaat gaacctgcaa
     7321 ttgaagggtc gaagagtgga a
//
LOCUS       DAOOOI010000091         7311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_1991_length_7311_cov_36.530458, whole genome shotgun sequence.
ACCESSION   DAOOOI010000091 DAOOOI010000000
VERSION     DAOOOI010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7311
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1991_length_7311_cov_36.530458"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            489..>7311
                     /locus_tag="PKX59_09420"
     CDS             489..>7311
                     /locus_tag="PKX59_09420"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HOZ91124.1"
                     /translation="MTKTQYNSKAKSASFWRWIMCFMFLLGIWTTGAQAQTTVAVGPT
                     TVSASSYFYGPVYRSSASSTFHYSMYSYIYSASEIAAAGIVSGSFINDIQFNLNAAAT
                     LSGAHTGNFEIWIQNSSATDQTSGTSWSTITGTATQVVAQTWNNTNPGSGGWKTFTFN
                     SPFAYTGQSLIVSVRWVLIPSGGSPYASGAFNWQYYTGPLITPAAAIGAASGSAQSGS
                     TALSTVSYGGNRRPLTQMSYTAGSPCSGTPTPGNTNSNPTSACVGQTISLSLQNLTMG
                     SGVTYDWQNSPDGVTWTSTGGTLPTYSTTQVASTWYQCVVTCTNSGLSTTSTPVQVSM
                     NPFYSCYCDPTYSSGTGFGDYISLVAIPTTTLNNPTTGAPSPYFTVYPQSGNTTCSLV
                     AGANYTMNVAGGTYSSCYIRGWIDYNQDGNFDASETIGVSGNVGSLTTGSFNFIVPVT
                     ALTGTTRMRIRSSDTSPGPSSSQVCGAANSTYGEAEDYLITLLAANACTGTPAASATT
                     ASVSGVCSGGNVDLATNPVYTDGGLTFDWQSSPDGITYSSTGVTTQTYNVTGFSATTW
                     YQCVVTCTNSGLSTTSTPVQVLLNSFYNCYCASIATYTGDEDIFNVTFGTLNNTSTCS
                     STGPGVGSVNQRYANYASGTGAPAAPNVIKGSTVPFSVQIGTCGSNYSNMTKIYIDWN
                     QNGSYADAGEEVYVSPTYTSGPHTETGNILIPMTAVAGTTGMRVITVETTSASSITPC
                     GSYGWGETEDYLVNVIQPVPCSGAPAPGLTVASVNPACFVNPLVLSLQNPMTDLGLTF
                     NWLTSPDGVTWSSTGVTTSTLTVNQSAATYYMCVVTCTNSGQSGNSTALLVNQTVCYC
                     ETPVYSYNSGYNMCTYYDYIGNVTFAGINNTTVCDNTPPYYNYYSSQTATVNRTGSYQ
                     VTISATAGGASFQLYKVYIDYNDNGSFDDAGEMVYTSGFISTATNFTQGNIVIPMTAP
                     LGTHRMRVRSTNPLNTNIDANSCSDDGYLGEVEDYNIVVNPAPPCSGTPAPGATLSDK
                     ALVCSSTTINLSLQNATPGTGVTYQWQSAVDVAFTTPVNLGTAATQTATQTATTYYRC
                     LVTCSGNTGISTPVLATSTTACYCTNPTTGSDGCALGLYINNVTFAGINNTSGCFSAV
                     PYTTPFYSFFPTQLATAQQGNTYAFSASAPNNPGSYYQWYGVWIDYNDNGSFDDAGEL
                     VLSSIAGASTTNNQLTGNITIPLTATAGQHLMRVRTGNVSVAINSCSDNSVSGEVEDY
                     LIDIIAAPQCSTPAPGNTIASATSVCANENLDLSLQNNNIALGLSYQWQVSTVGASGP
                     WTNVGTNANTYTATQSVASWYQCIVSCSYTSISTTSTPVGVTMNSVASCYCVAGASID
                     GCGTGNEYVSNVTFNTINNTSTCSGVALQYQDFTAISTNVQAGQSYTASVMITNYFSS
                     DQATVWIDFNQNGSFADAGEQFNLSGGASGVAFTGSITIPGNAVTGVTRMRVRGNYSG
                     AMNPCGITSYGEVEDYSLNITPPVCIFAPTYPADAGSACPDAITNTVQLSWPSLPAAT
                     GYDVYFGTNNPPTTVVSPNQPGLTYVANVTSGTQYYWMIVPQMPGGGTSCSVWSFTVS
                     PPPMPIASSGGDVCEGSDIFLSADNVDPGQLTGNTYSWTGPNSFSSALQNPSINSPSA
                     AYSGTYSVIVTNQYGCTASASTSLSVNPNPTITIDSVQNVSCLGGSDGIIYVSGSGGL
                     APYSFTSDFVNFNATGVMNNLPTGSSIIYAADGNACQAQITGNLTAISTAPPSQSVVV
                     TPTIIGMPAYACPGTTANLSIPAVPGATKYIWDGPFGTFFNGNPMNQSPYTTTTPSVQ
                     ITFSSSTTSFVNVGVQAANGCGASLRKIQKVRYQISTPKEITGATTMCASTNGTYTIA
                     PVTDATSYQWMITGNATVVPSGTSAVVFFGPSWNGGTLCVAAKTPCYTTAYKCIAISK
                     SASALNAITGPLSSCPNEVQTFNVLPCTGAASYNWTLPAGATGSSSTNSITATFGPTF
                     TAGGNICVSVTSICGVTSTPKCRTVAQGLPSVPTSISGITNGLCNQSVNYTVPSSPGV
                     TYNWTAPGTVIGNGNSSVSVTYGALTTGQLCVTASNSCGTSAARCTPLKGAPNSPIAL
                     TAIPASWCANTQGIEFTADLGNTTGSYTLSWGYPSSPTASYVAGGGNSNALTLDWGTG
                     NGSVVVTATNSCGAGSKAFAVTIGCKEGEMAKANQLNVYPNPTAGVLNVEYTAEKGTA
                     QVTVLDLSGRVVMTQTHANAAGQNTL"
BASE COUNT         2276 a         1479 c         1511 g         2045 t
ORIGIN      
        1 ggcagcaaca atcaggtgag agtggtagtg gagtaaaagt ttaaagattg acaatttaaa
       61 aagattgtca gaaaattaaa ggggagaagt cgaaaggctt cttccatttt tttatttata
      121 aattactata agaaattaaa aatcgcactt accaaaaatt taatcaattg atgttaaaaa
      181 gcgttacatc ttgctgtttt tattaataaa tattacagaa ttattagaaa tgttacgtgc
      241 taattaaaac attgataatc aataataaag tgatatttat aatatataac agaagatttt
      301 attttaaaaa taatttggaa tattaaaaaa tgatgaataa tattgaaggc gaaaaccaaa
      361 cttatatgct gttaaaaaga cgagtgctaa ctacaatttg taattatttt tcacaacaac
      421 ggcagaatta gtttttgaaa atcagaaaac aaaatcaaaa cataagttaa accaaaaatc
      481 aaattatcat gacaaaaaca cagtacaaca gtaaagcaaa gtctgcttca ttctggaggt
      541 ggattatgtg tttcatgttc ctgcttggaa tatggactac gggcgcacaa gcgcaaacaa
      601 cagtagcggt aggtcctact actgtatcag caagtagtta cttctatgga cctgtttata
      661 ggtcatctgc atctagtaca tttcattatt caatgtacag ctatatctat agtgcttcgg
      721 aaatagcggc agcaggtata gtaagcggaa gttttattaa tgacatccag tttaatttaa
      781 atgcagcagc tacattaagt ggtgcgcata ctggaaattt tgaaatctgg atacaaaaca
      841 gcagtgcaac agatcaaacc tcaggcactt cttggtccac aataacaggt acagcaacgc
      901 aagtagttgc gcaaacctgg aataacacaa atcccggttc cggtggttgg aaaactttta
      961 ctttcaattc accttttgcg tacacaggtc aatcattaat tgtttctgta cgatgggtat
     1021 taatcccttc aggaggatca ccttatgcaa gtggtgcatt taactggcaa tattatacgg
     1081 gtccattaat aacacctgca gcagcaattg gtgcagcaag tggttcagct caatcgggta
     1141 gcactgcttt aagtacagta tcatatggtg gaaacagacg tccattgaca caaatgtctt
     1201 atacagccgg ctctccttgt tcaggaaccc caactccggg taatacaaat tctaatccta
     1261 catctgcttg tgttggtcaa acaatatcat tatcactgca aaatctaact atgggctcag
     1321 gggtaactta tgattggcaa aactcacctg atggtgttac atggacaagt acaggtggta
     1381 cattaccaac ttattctacc acacaagttg catctacctg gtatcaatgt gttgttactt
     1441 gtacaaacag tggattaagt acaacctcca ctcctgttca agttagtatg aatccatttt
     1501 atagttgcta ttgtgaccca acttattctt ctggcactgg atttggagat tatatttcat
     1561 tagtggcaat tccaacaaca accttaaata atccaacaac aggagcacct tcaccttact
     1621 ttacagtcta tccgcaatca ggaaatacaa catgttcact tgtagctggt gcaaattata
     1681 caatgaatgt tgccggtggt acttattcat cttgttatat aagaggttgg attgattata
     1741 atcaggatgg taattttgat gcatcagaaa ctattggcgt ttctggtaat gttggttcac
     1801 taaccactgg aagttttaat tttattgtgc cggtaactgc tttaaccggt actaccagaa
     1861 tgagaatacg ttcaagtgat acttcaccag ggccatcaag ttcgcaagta tgtggagcag
     1921 ccaatagcac ttatggtgaa gcagaagatt atttaataac tttattagca gcaaatgctt
     1981 gtacaggaac acctgcagca tcagcaacta cagcatcagt aagcggagta tgttcaggtg
     2041 gtaatgttga tcttgcaaca aaccctgtct ataccgatgg cggcttgaca tttgactggc
     2101 agagttcacc tgatggaatt acttattctt caacaggagt tacaacacag acttacaatg
     2161 taactggatt ttctgcaaca acatggtatc aatgtgttgt tacttgtaca aacagtggat
     2221 taagtacaac ttcaactccg gtacaggttt tattgaattc attctacaat tgttattgtg
     2281 catctattgc tacatataca ggcgatgaag atatatttaa cgtaacattc ggaacattaa
     2341 ataacacttc aacatgttca agtaccggtc ccggtgtcgg ctctgtgaac cagcgttatg
     2401 caaactatgc cagtggcaca ggagcaccgg ctgcacctaa tgttatcaag ggttcaaccg
     2461 tacctttctc tgttcagata ggtacttgcg gaagcaacta cagtaacatg accaaaattt
     2521 acattgactg gaatcaaaat ggttcttatg ccgatgccgg agaagaagtt tatgtttctc
     2581 caacatatac aagtggtcca cacacagaaa ccggaaatat tttaatcccg atgactgctg
     2641 ttgccggaac tacaggaatg cgtgtaatta cagttgaaac aacttctgcc tcaagcatta
     2701 cgccttgtgg ttcctatgga tggggcgaaa cagaagatta tttggttaat gttattcaac
     2761 ctgtaccatg tagcggggca cctgcaccgg gtttaactgt tgcaagtgta aatcctgcat
     2821 gttttgttaa tcctttagtt ttaagtctgc aaaatccaat gaccgattta ggattaacat
     2881 ttaattggtt aacttctcct gatggtgtta cctggtcttc tacaggtgta acaacttcaa
     2941 cattgacagt taatcagtca gcagctactt attacatgtg tgttgttaca tgtaccaatt
     3001 cagggcaatc aggaaactct actgcattat tggttaacca aacggtttgt tattgtgaaa
     3061 ctcctgtata tagctacaat tcaggctaca atatgtgtac ttattatgat tatattggaa
     3121 atgtaacatt tgcaggtatt aataacacta ctgtctgcga taatacgcct ccatattata
     3181 attattattc atcacaaaca gcaacggtta acagaacagg aagttatcag gtaacaattt
     3241 ctgcaacagc cggtggcgca tcattccaat tgtataaagt ttatattgac tacaatgaca
     3301 atggtagctt tgatgatgca ggtgaaatgg tttacacttc aggtttcatc agtactgcaa
     3361 ctaactttac tcagggaaat attgttattc caatgactgc tccattaggt acacaccgta
     3421 tgagagttag atcaacaaat ccattaaata caaacattga tgccaattct tgttcggatg
     3481 atggttatct tggtgaagta gaggattata atattgtagt taatcctgca cctccttgta
     3541 gcggtacacc ggcaccgggt gctacattat ctgataaagc acttgtttgt tcatctacaa
     3601 caattaacct gagtttgcag aacgctactc cgggtacagg tgtaacttat caatggcagt
     3661 cagcagttga tgttgcattt accactccgg taaatttggg tacagctgca acacaaacag
     3721 caacacaaac agcaacaaca tattatcgtt gtcttgtaac ttgtagtggt aacaccggaa
     3781 tttcaactcc ggtattagca acatcaacaa ctgcatgtta ctgcaccaat cccacaaccg
     3841 gtagtgatgg ttgtgctctt ggtttgtata tcaacaatgt aacttttgca ggtataaaca
     3901 ataccagtgg ttgttttagt gcagtaccat atacaacacc attctatagc ttcttcccaa
     3961 cccagttggc aacagcgcaa caaggtaata cctatgcttt ctctgcttct gcaccaaaca
     4021 atcccggctc atactatcaa tggtatggtg tttggattga ttataatgac aacggaagtt
     4081 ttgacgatgc cggtgaattg gtgttgtcaa gtattgcagg agcttcaaca accaataatc
     4141 aacttacagg aaatattacc attcctttaa ctgctacagc tggtcaacac ctgatgcgtg
     4201 ttagaaccgg taatgtatca gttgcaatca attcatgttc tgacaactct gtaagtggtg
     4261 aagtagaaga ttatctgatt gatattattg cagctccaca gtgttcaacg cctgcaccgg
     4321 gtaatacaat agcatcagct acttctgtat gtgcaaatga gaacttggat ttgagtttgc
     4381 agaataataa tatcgcatta ggtttatctt atcaatggca ggtatctaca gttggtgcca
     4441 gcggaccatg gacaaatgtg ggtacaaacg caaataccta tactgcaaca caatcggttg
     4501 cctcctggta tcagtgtatt gttagttgtt cttatacaag tatttctact acttctacac
     4561 ctgttggtgt aacaatgaat tctgttgcca gttgttattg tgtagcagga gcttctatag
     4621 atggttgtgg aacaggtaat gagtatgtga gtaatgtaac atttaacaca atcaataaca
     4681 catcaacatg ttctggtgtt gcactgcagt atcaggattt cacagcaatt tcaacgaatg
     4741 ttcaggctgg tcaatcttat acagcttctg taatgattac aaactatttc tcaagtgatc
     4801 aggcaacagt ttggattgac tttaatcaga acggatcctt tgcagatgca ggtgagcagt
     4861 ttaatctttc aggcggtgca tctggtgtag catttacagg aagtataact attcccggaa
     4921 atgcagttac aggagtaaca agaatgagag ttcgcggaaa ttattccggt gcaatgaacc
     4981 catgtggtat tacatcttat ggtgaagtag aagactatag tctgaatatt acaccaccgg
     5041 tatgtatttt tgcaccaact tatcctgcag atgcaggaag tgcatgtcct gatgccatta
     5101 ccaatacagt tcagttaagc tggccatctt tacctgcagc tacaggttat gacgtgtatt
     5161 tcggcacaaa caatcctcca acaacagttg tatctccaaa tcagccgggt ttaacttatg
     5221 ttgctaacgt aactagtgga acacagtatt actggatgat agtaccacaa atgccgggcg
     5281 gaggaacctc atgtagcgta tggtcattta cagtatcacc accacctatg cctatagcaa
     5341 gttcaggagg cgatgtgtgt gagggcagtg acatcttctt gtcggcagac aatgtagatc
     5401 ccggtcagtt aacgggcaac acctattcat ggacgggccc caatagcttt agcagcgcct
     5461 tacaaaatcc atctatcaac agcccaagcg cagcctatag tggcacctat tcagtaatag
     5521 taacgaatca gtatggctgt acggcaagtg caagcaccag cctgagtgta aacccaaatc
     5581 caacgataac aatagattca gtacagaatg taagttgttt aggcggcagt gatggtatta
     5641 tttacgtatc aggatcagga ggcttagcac cctatagctt cacgtcagat tttgtgaact
     5701 ttaacgcaac aggagtgatg aataatttgc ctacagggtc aagtattatt tatgctgcag
     5761 atggcaatgc ctgtcaggca cagataacgg gtaatttaac agcgataagc acggcaccac
     5821 catcacaatc ggtagttgta acaccaacga ttataggcat gccagcctat gcctgtccgg
     5881 gtactacggc aaacttaagc atacctgcag ttcccggtgc aacgaagtat atctgggatg
     5941 gcccattcgg taccttcttt aatggtaatc cgatgaacca gtcgccatat acaacaacaa
     6001 caccaagtgt gcagataacg ttcagttcat caacaacatc gtttgtaaat gtaggagtac
     6061 aggcagccaa tggctgcgga gcatcactgc gtaagatcca gaaggttaga tatcagataa
     6121 gcaccccaaa ggagattacc ggagcaacaa caatgtgtgc cagcactaat ggaacatata
     6181 cgatagcccc tgtaacagat gccacaagtt atcagtggat gataacaggc aatgccacag
     6241 tagtgcccag tggcacctca gcagtagtgt tctttggccc aagctggaat ggaggcacgt
     6301 tatgcgtagc agccaagaca ccatgctata ctacagcgta taagtgtata gccataagta
     6361 agtcggcctc agcattgaat gctatcactg gtccattgtc atcatgtcca aacgaggtac
     6421 agacctttaa tgtactacca tgtacaggag cagcgagcta caactggacg ttaccggcag
     6481 gagctaccgg cagttcatca acaaacagca tcacagccac gtttggtcca acgtttacag
     6541 caggtggcaa catctgtgta agtgttacaa gcatctgtgg agtaacctca acacctaagt
     6601 gcagaacggt agctcaaggc ttgccatcag taccgacaag catcagtggc atcaccaatg
     6661 gattgtgtaa tcagagtgtg aactacacag taccatcatc tccaggcgtt acgtataatt
     6721 ggacagcacc cggcacagta ataggtaatg gcaacagttc cgtaagtgtt acttatggag
     6781 cattaacaac aggtcagttg tgtgtaacag cgagcaacag ttgtggcaca agtgcagcaa
     6841 gatgtacacc actgaagggg gcaccaaact caccgattgc attaacagcc atacctgcaa
     6901 gctggtgtgc caatacacag ggcatagagt ttacagcaga cttaggtaac acaacaggtt
     6961 cttatacatt atcatggggc tatccatcat caccaacggc aagttatgta gcaggtggag
     7021 gaaactccaa tgcactaacg ttggattggg gcacaggcaa tggctcggta gtagtaacag
     7081 cgacaaacag ttgcggagca ggctccaaag cttttgcagt gaccatagga tgtaaagaag
     7141 gtgagatggc aaaagccaat cagttaaatg tatatccaaa cccaacagca ggcgtgttga
     7201 atgtagaata cacagcagag aagggcacag cacaggtaac agtgctagac cttagcggcc
     7261 gtgtagtaat gacacagaca catgccaatg cagcaggaca aaacacatta c
//
LOCUS       DAOOOI010000092         7271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2001_length_7271_cov_24.274529, whole genome shotgun sequence.
ACCESSION   DAOOOI010000092 DAOOOI010000000
VERSION     DAOOOI010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2001_length_7271_cov_24.274529"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..778
                     /locus_tag="PKX59_09425"
     CDS             248..778
                     /locus_tag="PKX59_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015022883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOZ91125.1"
                     /translation="MSETYKTFPGGLYFVTFTTVGWLDIFTRRVYQDILINSLIYCQQ
                     DKGLQLYCYCVMPSHVHIIAKRKEGILNDLIRDMKAFSSKKLMDAIEENMLESRREWL
                     IKEFKYYGKISPHKQYRQFWQHNNHAFELYSNRIIDQKINYIHNNPVEAGFVDEPHLW
                     RLSSANENSPVKVLSM"
     gene            complement(938..1204)
                     /locus_tag="PKX59_09430"
     CDS             complement(938..1204)
                     /locus_tag="PKX59_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91126.1"
                     /translation="MKKVMIAAAALIIGGTAFANAQTNAKPAATTTHQAAPAATHETA
                     AQKKEVKEEKMEKKEATPAKHETKKPAAHKSHAKKVEKKEESAK"
     gene            complement(1455..1889)
                     /locus_tag="PKX59_09435"
     CDS             complement(1455..1889)
                     /locus_tag="PKX59_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91127.1"
                     /translation="MKRIMLLAGLCLALTATMRAQVVQKNEISDQAQQQLLQLNDEMA
                     TSFQHTDLLKVSEFFDDDATIYMNGNKVTGRKALSDFWNKITARKKMEINVDEMNGTG
                     KFIYETGTLTMNTENNTVTKNFVMVWKRLPNFDYKIVVAGFN"
     gene            complement(1991..3691)
                     /locus_tag="PKX59_09440"
     CDS             complement(1991..3691)
                     /locus_tag="PKX59_09440"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOZ91128.1"
                     /translation="MNKTVTVNIGGIVFHIEENAYDMLGSYLQAIRNHFTISDGRDEI
                     IQDIESRIAEIFQQNLGTARQVVTADDVNQAIGIMGRPEDFGATEEASANNASASSEQ
                     NNQSDHFFRRRLYRDTDDKVIAGVCSGIGHRLGIDAVWMRLLFIISVFFAGSGIGVYI
                     ILAIVMPKAITPTQKLEMRGDPVNISSIRRETESPGSTKQPSAVSGVFDTLGDLIKGL
                     FKFVVYAIMAILAFAGLMMLIGIGLFILAMLGVSGLTIPIYISDQFLSSSQQWWVSLS
                     VLLLIGVPAIWLMTLLIKWIFRIKYNNRFLNVAAGMLFMAGIVIAFFTGLNIARDFAF
                     DGKVRNTVALITPPSPTMYLKLMDDPKYDEEDNSKFFHRSGRNYSITTGSSFEFNSDD
                     VQFTVQKSPSDKFELVRVFSANGTTENLAVESARGIIYNFEQKDSLLMFSNHFPLPKG
                     TKFRNQRVDLVLKVPVGSSIFLDESMEEIIRDIPNVTDTWDGDMGGHTWKMTENGLIC
                     TDFEFQKGDSTDIHNPENVDISIDNNGVRIKGKEAKSGDKDVNINIGKDGVHIKSTDK
                     "
     gene            complement(3688..3999)
                     /locus_tag="PKX59_09445"
     CDS             complement(3688..3999)
                     /locus_tag="PKX59_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOZ91129.1"
                     /translation="MRKGVLEFCILSIISKGEVYPSDIIEKMKEARLIVVEGTLYPLL
                     TRLKNSGLLSYTWIESNAGPPRKYYKLTPLGKEHLKELKKTWDELVDAVSQTYQNSTE
                     E"
     gene            complement(4350..5948)
                     /gene="ftsZ"
                     /locus_tag="PKX59_09450"
     CDS             complement(4350..5948)
                     /gene="ftsZ"
                     /locus_tag="PKX59_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOZ91130.1"
                     /translation="MNYEFMPKELPSIIKVIGVGGGGSNAVNHMYKLGIKGVDFVVCN
                     TDAQALEMSPVPRKIQLGPSLTEGRGAGSIPEVGKQSAIENIEELKAILSDNTKMVFI
                     TAGMGGGTGTGAAPIIAGVAREMGILTVGIVTVPFAFEGRKRKQQADAGIEELKNSVD
                     TLLIICNDKLREIHGNLKLNTAFGYADNVLATAAKSIAEVISVTLHINVDFADVQTVM
                     RDSGVAIMGAAQSSGEGRAIRAAELALDSPLLNDNNIEGARHILLNITSGKEEITMDE
                     MGDITDYIQQKAGQTAEIIMGVGTDDSLGDKISVTIIATGFNADKEKTKEEKVKQPEK
                     VVLNIYDETPTSVVNEVSETTVEEEPVMNEPVLITLEQDIPVTSTAFFEKDNTPEEVV
                     ADKSTMEFEFTGFPNVISQDEEITAPEATLTEEPTVSELPKSQESLPEVPSQESREPN
                     SDIYRRTRERIETMKSSLNSRTLNPQALNEMEKEPAYMRKNVKLQNVLSSGDTNYSRY
                     SLNNEQDKTPGLSKGNKFLHDRPD"
     gene            complement(5989..7251)
                     /gene="ftsA"
                     /locus_tag="PKX59_09455"
     CDS             complement(5989..7251)
                     /gene="ftsA"
                     /locus_tag="PKX59_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOZ91131.1"
                     /translation="MVGLDIGTTKIAAIVGRRNEFGKIEIVGTGRAESVGVTRGVVAN
                     IEKTVGSIKQAVAEAEHKCGGNIKSVHVGIAGQHIKSLQHRGMITRSSTDDEISQRDI
                     DALIDDMYKLAMPPGEEIIHVIPQEFIVDNEQGIKDPIGMSGIRLEANCHIITGLVTA
                     ANNIHKCVTKAGLTPADLILEPLASAEAVLTEEEKEAGIVLVDIGGGTTDVAIFQDNI
                     IRHTAVIPFGGNVITEDIKEGCHIIRNQAELLKIKFGSALSSQMKDNEIVSIPGLRGR
                     EPKEISVKNLASIIQARVEEIIEQVYYEIKTSGFEKKLIAGIVVTGGGAQLRHITQLM
                     EYITGMDTRLGYPNEHLAKSAEETKSPIYATGVGLVIKGFQDLDKHRKSGQKNTSEPR
                     VQHKGSWFSGIFDSTKKIFTDDSDDKII"
BASE COUNT         2001 a         1546 c         1337 g         2387 t
ORIGIN      
        1 atttttttat ccatgtttta cactttttag tgtaaaccta tttcaggaca agacagcatt
       61 gaacaacacc gtacccgcaa agcccctgct tcgtgggcct tgtgtttaga agctgtgctt
      121 cgaacagcaa tgaactgtac ccgcgaagct cctgcttcgt gggccttgtg tatagaagct
      181 gtgctttgaa caccaatgaa caacattata aataaaatta caacatttgt ataaacataa
      241 atatataatg tcagaaacat ataaaacatt tcctggaggg ttatactttg taacttttac
      301 cactgtgggc tggttagata tatttacaag aagagtttat caggatatac taattaatag
      361 tctaatctac tgccaacaag acaaaggctt gcaactgtat tgttattgtg taatgccaag
      421 tcatgttcac attattgcca aaaggaaaga aggaatattg aatgacctga tacgtgatat
      481 gaaagccttt tcgtcaaaaa aacttatgga tgccatagaa gaaaatatgc ttgaaagcag
      541 acgtgagtgg ctaattaaag aatttaaata ttatggtaaa atatcacccc ataaacaata
      601 caggcagttt tggcaacata acaatcatgc atttgaatta tatagcaaca ggataataga
      661 tcaaaaaata aattacatac ataacaaccc ggtggaagcc ggttttgtag atgaaccgca
      721 tttgtggcgg ttaagtagcg ctaatgagaa cagccccgtt aaagtgttga gcatgtaatg
      781 caaagcacag cttttaggta cttaacacca tgtaatgcaa agcacagctt ttatgcattt
      841 atcccacgaa gcaggagctt cgcgggtacg ggggtgtata ttaaaaaaaa agaggctgcc
      901 tcaaaaaatt gagacagcct cacgtttcaa aatcaattta cttagcgctt tcttcttttt
      961 tctctacttt tttagcgtgg ctcttgtgcg cagcaggttt ttttgtttcg tgctttgcag
     1021 gtgtagcttc ttttttctcc atcttctctt ctttcacttc ttttttctgt gccgctgttt
     1081 catgtgttgc ggcaggtgca gcctgatgtg tcgtagtagc tgcaggtttt gcattagttt
     1141 gcgcattggc aaaggcagtg cctcctatta taagggcagc tgcggcaatc attacttttt
     1201 tcattttatt tatttttttg ttttgaaatg atgggtcaaa tgttcaattg ccaacatgat
     1261 gttgatatga aaatttgcac atcttcattt ttgtgataat cagacttcat aatcccgtca
     1321 tccatgaagt taatgtttaa caatcacaag aattgatatt atccaagcta agattaaaac
     1381 aacaagaggt ctgcaaataa aatacagacc tctgttatac aaacacaaat aatcagttac
     1441 tcaaactaat ttttttagtt aaacccggca acaacaattt tataatcgaa gttgggcaaa
     1501 cgtttccaca ccattacaaa gtttttagtt acggtattat tttctgtatt cattgtaagc
     1561 gttccggttt cgtaaatgaa tttgcctgta ccattcattt catcaacatt aatttccatt
     1621 ttttttctgg cagtaatttt attccagaaa tcagacagtg cctttcggcc ggttacctta
     1681 tttccattca tgtagatggt ggcatcatca tcaaagaact cgctcacctt tagtaaatct
     1741 gtatgctgaa atgatgtagc catttcatca ttgagttgca gcaactgttg ttgtgcctga
     1801 tcgcttattt catttttctg aaccacctgt gcgcgcattg tcgcagtgag tgcaaggcat
     1861 agccctgcca ataacataat tcgcttcata atatttgatt taaaatttag ttgatattga
     1921 ttgataaagg aattaacctt tatgtgtagg acgaatacta tcctgaattg ttacagcgtt
     1981 gtagaggaat ttatttatcg gttgacttaa tatgtacacc atcttttccg atgttgatat
     2041 ttacatcttt atcaccactc tttgcctcct tacccttgat gcgcacacca ttgttatcaa
     2101 tactgatgtc cacattttcg ggattatgaa tatcggtgct gtcgcctttc tgaaattcaa
     2161 aatcagtaca tatcaaacca ttctctgtca tcttccaggt atgtccgccc atatcgccat
     2221 cccaggtgtc ggttacgttt ggaatatctc tgataatttc ttccatactt tcatccagaa
     2281 aaatgctgct gccaacagga actttcaaaa ccaaatcaac acgctgatta cgaaacttgg
     2341 ttccctttgg caatggaaaa tgattgctga acatgagtaa agaatctttc tgttcgaagt
     2401 tataaatgat gcctcgtgca ctttcaacgg caagattctc tgttgttccg tttgcagaaa
     2461 acactctgac caattcaaac ttatctgatg gcgatttctg aacagtaaac tgcacatcgt
     2521 ctgaattaaa ctcaaagcta cttcctgtag tgatgctata gttacgacca ctgcgatgaa
     2581 aaaatttact gttatcttct tcatcatatt tagggtcgtc catcaacttt aaatacatag
     2641 ttggcgaagg cggtgttatc aacgcaacag tattacgcac cttaccatcg aaggcaaaat
     2701 cgcgtgcaat atttaatcct gtgaagaagg caataactat acctgccata aacaacatgc
     2761 ctgcagcaac atttaaaaag cggttgttgt attttattct gaaaatccat ttaataagca
     2821 aggtcattaa ccaaatggcc ggaacaccga tgagtagcaa cactgataaa gatacccacc
     2881 actgttgcga gcttgacaga aactgatcgg atatgtagat tggaatagtg agtccgctaa
     2941 caccaagcat tgccaaaata aacaagccaa ttccaatcaa catcataagt cctgcaaatg
     3001 ctaatatggc catgatggca taaacaacaa acttgaaaag tcccttaatt aaatcgccaa
     3061 gagtatcaaa tactcctgat actgcagaag gttgtttggt ggaaccgggc gattctgttt
     3121 cgcgtctgat gcttgaaata tttacagggt cgccacgcat ttcgagtttc tgagttggtg
     3181 taatggcttt gggcataact attgccagaa tgatgtaaac cccaattcca cttcccgcaa
     3241 aaaatacact aatgataaag agcaagcgca tccacactgc gtcaatgccc aaacggtggc
     3301 caatgccgga acaaacacct gcaataactt tatcgtcagt atcgcgatac agcctgcgtc
     3361 tgaagaaatg atcggactga ttgttttgtt cactgcttgc tgatgcattg tttgctgatg
     3421 cttcttctgt tgcaccaaaa tcttcaggtc gtcccataat gccgattgcc tggttcacat
     3481 catcagcagt aacaacctgc cttgctgtgc ctaagttttg ctggaatatt tcagctatac
     3541 ggctttcaat gtcctgaata atttcatcgc gtccatcact gatggtaaaa tggttgcgga
     3601 tagcctgcag ataactgcca agcatgtcat aggcgttttc ttctatatgg aaaactatgc
     3661 ctccgatgtt tactgttact gtcttgttca ttcttcagtt gagttttggt aggtctggct
     3721 gacagcgtca accagttcat cccatgtttt ttttaattct tttaaatgtt cctttcccaa
     3781 tggagttagt ttgtagtact tgcggggagg tcctgcattg ctttcgatcc aggtgtagga
     3841 taacaaacct gaatttttta atcgtgtcag caatggataa agtgttccct ccaccacaat
     3901 gagtctggct tctttcattt tttcgatgat atcgctggga taaacttctc cttttgatat
     3961 gatggataaa atgcagaact ccaaaactcc tttgcgcatt tgcgactgtg tgttttcgag
     4021 attacccacg ttctttagat ttttgattgt ttaattatat tcttttacta aatgactatg
     4081 caaagatata ttgattaaac agtaccatgc aacacatagt actaaaatat tttatatttt
     4141 attgattttc aggttattta ttttttatta gggtaatgtt agggctaaag cccttgggct
     4201 tggcagaggc ttcctatcgc ctcactctaa agagtgaggt gagttgggat gataggagtg
     4261 ttcgctagcc tcaccatgaa gggtgaggtg aaaaagaaaa gccggaacag gatactcctg
     4321 ttccggctta tataagttgt aatcaaacac taatcaggtc tgtcgtgcaa aaacttattg
     4381 cctttgctaa ggcccggagt tttatcctgc tcattattga gtgagtagcg cgaatagttt
     4441 gtatcccctg acgacagtac gttctgcagt tttacattct tacgcatata tgcaggctct
     4501 ttttccattt cgtttaacgc ctgcggattc aatgttctgc tgttgagtga gcttttcata
     4561 gtctcgattc tttcgcgtgt gcgtctataa atatcgctat taggctctct gctttcctgc
     4621 gatggcactt cgggcaacga ctcctgcgac ttcggcaatt cgctaaccgt tggctcctct
     4681 gttaatgtag cttcgggtgc tgttatttcc tcatcctgag aaatcacatt cggaaatccg
     4741 gtgaactcaa attccatagt acttttatca gcaacaacct cctccggtgt attgtctttt
     4801 tcaaaaaatg ctgttgatgt aacaggtata tcctgctcta atgtaataag aacaggttca
     4861 ttcattacag gctcttcctc tacagttgtt tcactcactt cgttaaccac agaagttggt
     4921 gtttcatcat agatgtttaa aacaactttc tccggttgtt tcactttttc ttccttagtt
     4981 ttttccttat cggcattaaa gcctgtggca atgatggtaa ctgaaatttt atcgcctaaa
     5041 gaatcatcgg taccaacgcc cataatgatc tctgctgtct gcccggcttt ttgctgaata
     5101 taatctgtta tatcgcccat ttcatccata gtaatttctt ctttaccgct ggtgatgttc
     5161 aggagaatat gacgtgcccc ctcaatgttg ttatcgttga gtaatggtga gtcgagagca
     5221 agttctgctg cacgaatagc tctgccttcg ccagaagact gtgcagcacc cataatggca
     5281 acaccactgt cgcgcataac agtctgcaca tctgcaaagt caacattaat gtgcaaggta
     5341 acactgataa cttctgcaat actcttagca gcagtagcta acacattatc tgcataacca
     5401 aatgctgtat tcaactttaa gttgccatga atttcgcgca gcttatcatt acagataatc
     5461 agcaatgtat caacactgtt tttcagctct tcaataccgg catcggcctg ttgttttctc
     5521 ttgcgacctt caaatgcaaa aggcacagtt acaattccta cagtgaggat acccatttcg
     5581 cgggctacac ctgcaatgat aggtgcagca ccggttccgg ttccgccacc catgccagca
     5641 gtgataaaaa ccatcttggt attatcacta agaattgctt tcagctcttc aatattttcg
     5701 atggccgatt gtttgccgac ttcgggaata gatccggcac cacgtccttc tgtaagactt
     5761 ggcccgagtt gtattttgcg tggcacaggg ctcatctcca aagcttgtgc atcggtgttg
     5821 caaactacaa aatcaactcc tttgattccg agtttgtaca tgtggtttac agcattgctt
     5881 cccccaccgc ctacaccaat aacctttatg attgatggca gctcctttgg cataaattca
     5941 tagttcatca tcttaattta ttttttgttg gttatgaaaa tttttaactt aaattatttt
     6001 atcgtcagag tcgtcagtaa atattttttt ggtagaatca aaaattccgc tgaaccaaga
     6061 tcctttatgt tgtacacgtg gttcagaagt gtttttttgt cctgattttc tgtgtttatc
     6121 caaatcctga aaacctttaa tcactaatcc tacaccggtt gcataaatcg ggcttttggt
     6181 ttcttcggca ctttttgcca agtgctcgtt tggatagccc aatcgtgtat ccatgccggt
     6241 aatatattcc atcaactgtg tgatgtgtcg tagttgtgca ccaccaccgg tgacaacgat
     6301 acctgcaatt agttttttct cgaaaccgga agttttgatt tcataataaa cctgctcaat
     6361 aatttcttca acacgtgcct gaataatgct tgcaagattt tttacagaaa tttcttttgg
     6421 ttcacgaccg cgtaatccgg gtattgaaac aatctcattg tctttcatct gacttgataa
     6481 tgcagagcca aattttattt ttaataattc tgcctgattg cggataatgt ggcaaccttc
     6541 ttttatatct tctgtaatta catttccacc aaatggaatt acagcagtgt gccgaataat
     6601 gttgtcctga aaaatggcaa catctgtagt tccaccacca atatcaacta acacaatacc
     6661 tgcttctttt tcttcttcgg tgagaacagc ctctgcactt gccaaaggtt caagaattaa
     6721 atctgcaggt gtgagtcctg ctttagtcac acacttgtgg atattgtttg ctgctgtaac
     6781 aagtcctgta atgatatgac agttggcttc aagtctgatg cctgacatac ctatcggatc
     6841 ttttattccc tgctcattgt caacaataaa ttcctgtgga ataacatgaa taatttcttc
     6901 gcccggaggc attgccaatt tatacatgtc gtcaattaat gcatcaatat cacgctggct
     6961 gatttcatca tctgtgctgc tgcgtgttat catacctcta tgctgaaggc ttttgatatg
     7021 ctgacctgca ataccaacat gcacagattt tatattgcct ccacacttat gttctgcttc
     7081 ggcaacggct tgcttaatac tgcctacagt cttttcgata ttagcaacaa cacctcttgt
     7141 aacaccaaca gattctgctc ttccagtgcc tacaatttca attttcccaa actcgtttct
     7201 gcgacctaca atggctgcaa tttttgtagt gcctatatca agacctacta ttacttcatt
     7261 acctgttacc t
//
LOCUS       DAOOOI010000093         7166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2046_length_7166_cov_15.369850, whole genome shotgun sequence.
ACCESSION   DAOOOI010000093 DAOOOI010000000
VERSION     DAOOOI010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2046_length_7166_cov_15.369850"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PKX59_09460"
     CDS             complement(<1..328)
                     /locus_tag="PKX59_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HOZ91132.1"
                     /translation="MNILFSADRKTYFSLHNILGFYPIKLSLYKLAFSHRSQTKEVTN
                     GIKHSNERLEYLGDAVLGAVVAEMLFKKFPFREEGFLTEMRSKIVSRENLKHLAFKLG
                     INHFIKK"
     gene            complement(359..1612)
                     /gene="fabF"
                     /locus_tag="PKX59_09465"
     CDS             complement(359..1612)
                     /gene="fabF"
                     /locus_tag="PKX59_09465"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOZ91133.1"
                     /translation="MELKRVVVTGLGALTPLGNSVSEYWDGLLNGVSGACQITKFDAS
                     KFKTHFACEVKNFDPLVYLEKKEARKLDPFSQYAIAVSDQGVKDAGLDTANINHDRVG
                     VIWGSGIGGLRTFLDEVIAYAKGDGTPRFNPFFIPKMIADIASGHISMRFGYRGPNYT
                     TVSACASSTNALIDAFTYLRLGKADIIISGGSEASVNEAGIGGFNAMHALSERNDSPQ
                     TASRPFDLDRDGFVLGEGAGCIVLEELSHAQARGAKIYAEMVGGGMSADAYHLTATHP
                     EGLGAFNVMKSSLDDAGMTSADIDYINVHGTSTPVGDPSEIKAILKLFGDDAFKLNIS
                     STKSMTGHLLGAAGAIEAIASILAVQNNIVPPTINHFTDDPTFDPRLNFTFHKPQQRN
                     IRAALSNTFGFGGHNASVIFKKFTP"
     gene            complement(1624..1860)
                     /locus_tag="PKX59_09470"
     CDS             complement(1624..1860)
                     /locus_tag="PKX59_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOZ91134.1"
                     /translation="MSDIASRVKAIIVDKLGVDEGEVTNEASFTNDLGADSLDTVELI
                     MEFEKEFNIAIPDDQAEKISTVGQAVDYITANVK"
     gene            complement(2018..3013)
                     /gene="trpS"
                     /locus_tag="PKX59_09475"
     CDS             complement(2018..3013)
                     /gene="trpS"
                     /locus_tag="PKX59_09475"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOZ91135.1"
                     /translation="MDTVVSGIRSTGNLHLGNYFGAIKNFTAMQHEYRCFFFIADYHS
                     LTTHPTPENLQQNVKAALVEYLACGLDPEVCTMYIQSDLPETAELYLLLNMNAYVGEL
                     ERVTSFKEKIRKHPDNINAGLLTYPVLMAADILIHKATKVPVGKDQEQHLEMSRTFGN
                     RFNRLYKTEYFGEPMAFNFGKTLVKVPGLDGAGKMGKSESEGNALFLKDDAATIRKKI
                     MRAVTDSGPTEPNSAKPEAIENLFNLIKLVSSPDTISHFETAYNNCTIRYGDLKKQLA
                     EDMVTFMAPITQKIIDIEKNHDYVKKVVHLGREKAKASAQKTIEEVRQIIGIRRF"
     gene            3108..4379
                     /locus_tag="PKX59_09480"
     CDS             3108..4379
                     /locus_tag="PKX59_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOZ91136.1"
                     /translation="MIPLAERMRPKSLNEYIGQEHLTQAGAVLAKAIEKNTIPSMILW
                     GPPGVGKTTLAFILANSLSRQFYHLSAINSGVKEVRAVIETARQHFETVQQPSILFIG
                     EIHRFSKSQQDSLLGAVEKGIITLVGATTENPSFEVISPLLSRCQVYILKALTAEHLR
                     TILHAALTNDAVLKEHKVTIKEDDALLRFSGGDARKLLNLLELVIQVNQQNEICIDNK
                     MVEQTIHQKMALYDKTGEQHYDLISAFIKSLRGSDPNAAVYWLARMIHGGEDPLFIAR
                     RMLILASEDIGNANPNALLIANQCFDAAHKIGFPECRIILSQAAIYLACSPKSNSSYM
                     AIEEALELVAKTGDLPVPLHLRNAPTNLMKEIGYGKDYAYAHSYENNFVKLNYLPDQL
                     KGKKIYSPGKNIREAEFQQFLKIRWENEYDY"
     gene            4474..5808
                     /gene="creD"
                     /locus_tag="PKX59_09485"
     CDS             4474..5808
                     /gene="creD"
                     /locus_tag="PKX59_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HOZ91137.1"
                     /translation="MENTSHSFFDRLNQWIKNSVMVKLLSMAILILLLLIPSEMVQSL
                     INERQQTHDSALDEISSKWGFEQHLSGPVINVPYQSFFVDADGKRKSQIQYAHFLPEE
                     LKIAGTLEPEMRHRGIYEAVVYKSDVSLKGYFRFPDFSEWNIAPSDIMWKETTLSLGI
                     SDLRGIKQRLTINLNGNSIEFNPGVENNDVHESGVSIRLPIADSLNKPDFKFDMSFKL
                     NGSKEMYFMPLGKETTVNIKSDWTNPSFDGAFLPDTHHISDKGFDAEWKIFHLNRNYG
                     QKFRGAPTGLQESSFGVKLLIPVDNYQKTYRSAKYAILLITLSFTLFFFIEVLNRKRI
                     HPFQYILVGVALCLFYTLLLSVSEYISFNLSYLLSAAAIILLISLYAKTIFNNLRLAL
                     LFSLVLIILYVFVFSIIQLQDYALLLGSIGLFIVLALVMYLSRKINWYGSEK"
     gene            5792..6169
                     /locus_tag="PKX59_09490"
     CDS             5792..6169
                     /locus_tag="PKX59_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOZ91138.1"
                     /translation="MVQKNKGDHYQLGREGENMAVEYLIANGYKIVERNWRFGKLEID
                     IIASKGNLLCIVEVKTRTGDYFGEPEEGVTKAKERFLATAADHYIISKNLDVEVRYDI
                     VSIVIYNGRHELKFIEDAFYPYS"
     gene            complement(6171..6797)
                     /locus_tag="PKX59_09495"
     CDS             complement(6171..6797)
                     /locus_tag="PKX59_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOZ91139.1"
                     /translation="MENITESLEFKSSPALREKLISYGTTKKFNAGDVILNENAYIRS
                     IPIVLSGSIRVMRNDDEGKEILLYYIKSGESCIMSFLGGIHQDTSKVRAVAEDETEIL
                     FVPVDKVITLIREHDDWLDFIFRLYHKRFEELLEVVNAVAFKKIDERLILLLQKKVEL
                     SKSKTITVTHEQLANELGTARVVVSRLLKQLEVDGRVELGRNKITLLQ"
     gene            complement(6830..>7166)
                     /locus_tag="PKX59_09500"
     CDS             complement(6830..>7166)
                     /locus_tag="PKX59_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91140.1"
                     /translation="NMTSSQPLYTDNDFLRLITHCTSADEVKKVLSSHNIADADTKSS
                     ELFKKLCCIKRQTKGIYLMAAGAFMGFIGCLLSIVNPIPFLFDFALYGLTPGAVLVVL
                     IGLYLFIEG"
BASE COUNT         2192 a         1355 c         1388 g         2231 t
ORIGIN      
        1 ttttttttat aaaatgattt ataccaagtt tgaaggcaag atgttttaaa ttttcgcggc
       61 ttacaatctt gcttcgcatt tcagttaaaa aaccttcttc gcggaacgga aattttttaa
      121 aaagcatttc ggctacaacg gcaccaagca cagcatcacc aagatattct aatcgctcgt
      181 ttgaatgttt tatgccattg gtaacttctt tagtttgcga acgatggcta aaggctaact
      241 tgtataacga taactttatt ggataaaagc caagaatatt atgcagcgaa aagtatgttt
      301 tgcgatcggc tgaaaataaa atgttgatag gaaagaagcc gtaaaatctc actgattatc
      361 agggagtgaa ttttttgaaa attacagagg cattgtgtcc gccaaagccg aatgtattgc
      421 tcagcgctgc tctgatgttg cgttgttggg gtttatggaa agtaaaattc aaccgtggat
      481 caaaagtagg atcgtcagta aaatggttga tagttggtgg tacaatatta ttttgtactg
      541 ctaaaataga tgcaattgct tcaatagctc cggcagcacc taacaggtgt cctgtcatag
      601 atttggttga gctgatgttc agcttaaatg catcgtcacc aaaaagtttt agtatggctt
      661 taatctcact tgggtctccc acaggtgtgc ttgtaccatg aacattaatg tagtcaatgt
      721 cggcagaggt cattcctgca tcgtcaagcg aagatttcat tacgttaaaa gcaccaagac
      781 cttcaggatg tgttgctgta agatgataag catcggcact catgccaccg ccaaccattt
      841 cagcataaat ttttgctccg cgagcctgtg catgactaag ctcttcaaga acaatacaac
      901 ctgcaccttc accaagcaca aatccatcac gatctaaatc aaaaggacgt gaggcagttt
      961 gtggtgagtc gtttctctcg ctaagtgcat gcatggcatt gaaacctcca atgcctgctt
     1021 cgttaacaga agcttcagag ccacctgaaa taataatatc ggctttgcct aaacgcagat
     1081 aagtaaatgc atctatgagt gcattggttg atgatgcaca tgcagaaacc gttgtgtagt
     1141 tagggccacg atagccaaaa cgcatagaga tatgtcccga tgcaatgtcg gcaatcattt
     1201 taggaatgaa aaaagggttg aaacgtggtg ttccgtcacc tttagcatag gcaataacct
     1261 cgtcaagaaa agtacgaagt ccgcctatac ccgatcccca gataacacct actctgtcat
     1321 gattaatgtt ggcagtatct aagcccgcat cttttactcc ctgatcggaa acagcaatag
     1381 catattgcga aaacgggtca agcttgcgtg cttctttttt ctctaaataa acaagcgggt
     1441 caaaattttt aacctcgcag gcaaagtgtg ttttaaactt tgaagcgtca aactttgtaa
     1501 tctgacaagc accgcttaca ccgttgagta aaccgtccca gtattctgaa acagagttac
     1561 ctaatggtgt aagggcacct aatcccgtta ctacaactct cttgagctcc atgtaaggga
     1621 cgcttatttt acgttagcag taatataatc tacagcttgt cctacggttg agattttctc
     1681 ggcctgatca tccggaatag caatgttaaa ctctttttca aattccataa tcagttcaac
     1741 tgtgtcgagc gaatcagctc ctaaatcgtt tgtaaatgac gcttcattag ttacttcacc
     1801 ttcgtcaaca cctagtttgt caacaataat ggcttttact ctggatgcaa tgtctgacat
     1861 tttatataaa gttttgaatt agagcgcaaa ttaaatggtt ttgtaactga ttttcaaaaa
     1921 tattttcccc aacataatta attgttcttg gttgaatcag aggcacatac acacttatct
     1981 ggcaggtaac aattttttaa cttttcaaat cagggtacta aaaccttcta attcctatga
     2041 tttgccttac ctcttctatc gttttttgcg cacttgcctt ggctttttcg cgtccaagat
     2101 gaaccacttt ttttacataa tcgtggtttt tttcaatgtc aatgattttt tgggttatgg
     2161 gagccataaa agtcaccatg tcttcggcaa gctgcttttt cagatcaccg tagcggatgg
     2221 tgcagttatt atatgcagtt tcaaaatgac ttattgtatc aggtgatgaa accaatttta
     2281 tcagattgaa aagattttct attgcctcgg gcttagccga gttaggctct gtagggccgc
     2341 tgtcagttac agcacgcatt atcttttttc tgatggtggc tgcatcatct ttcagaaaca
     2401 aggcattacc ttcactttca gacttaccca tttttcctgc gccatcaagc ccgggaactt
     2461 ttaccaatgt tttcccaaag ttaaatgcca taggctcacc aaaatattct gtcttatata
     2521 agcggttaaa acgatttccg aatgtgcggc tcatttcaag atgctgctcc tggtctttgc
     2581 caacaggaac ctttgttgcc ttgtgaatta atatatcggc agccattaaa acaggataag
     2641 tcaatagtcc ggcattaata ttatcaggat gttttcttat tttttctttg aatgatgtta
     2701 ctctttccaa ctcacctaca taagcattca tattcaacag cagatagagc tctgcagttt
     2761 ccggcaaatc gctttgtatg tacatggtgc aaacttccgg atctaaacca caggcaagat
     2821 attcaaccaa tgctgccttt acattttgct gaagattttc aggtgtagga tgtgtggtga
     2881 gcgagtgata atcggcaata aagaaaaagc agcggtattc atgctgcatg gctgtaaaat
     2941 tttttattgc accaaaataa ttacctaagt gcaggtttcc tgttgatctt attccactta
     3001 ctactgtatc cataggcgca aaaatagatt aattgtatta acaggttttt attttagcat
     3061 aacagcaaag aattattatt taatttgggt tataaatatt gattgtaatg ataccattag
     3121 cagagcgcat gcggccaaaa tcgctgaatg aatacattgg tcaggaacac ctcactcagg
     3181 caggagcagt tcttgcaaaa gccattgaga aaaataccat tcccagtatg attttatggg
     3241 ggccaccggg tgtgggcaaa acaacactgg cttttatatt ggctaactct ttaagcaggc
     3301 agttttatca tctgagtgca ataaacagtg gtgttaaaga agtgcgggca gtaattgaaa
     3361 ctgcaaggca acattttgaa acagtacaac agccatcaat tttatttatt ggtgaaatac
     3421 accgttttag caaatcgcag caggattctt tacttggcgc tgtagagaaa ggaattatta
     3481 cactggttgg agcaacaaca gaaaatccat cgtttgaagt aatctcaccc ttgctatcgc
     3541 gctgccaggt ttatatttta aaagccctca ctgcagaaca tctcaggact attttacatg
     3601 ctgcattaac aaacgatgca gtacttaaag aacataaagt tactattaaa gaagatgatg
     3661 ctttactgcg tttcagtggt ggcgatgcca gaaaacttct taatctgctc gaattggtca
     3721 ttcaggtaaa tcaacaaaac gaaatttgta ttgacaataa aatggtagag caaaccattc
     3781 atcagaagat ggcgctttat gataaaacag gagaacaaca ttatgatttg atttcagcat
     3841 ttataaaaag ccttagaggt agcgacccca atgcagcagt ttactggcta gcaaggatga
     3901 ttcatggtgg cgaagatccg ttgtttattg caaggcgaat gctcattctt gcatcggaag
     3961 atattggtaa tgcaaaccct aatgctttgc ttattgccaa tcaatgtttt gatgcagcac
     4021 ataaaattgg ttttcctgaa tgtagaatca ttttgtcgca ggctgcaatt tatctagcct
     4081 gttcgccaaa aagcaattca agttatatgg ccattgaaga agctttggaa ttggttgcca
     4141 aaacaggcga cctgcctgta ccactgcatc tacgaaatgc acctacaaac ctgatgaaag
     4201 aaattggtta tggaaaagat tatgcttatg cacatagtta tgaaaataat tttgtgaaat
     4261 taaattatct gcccgaccaa ttaaaaggca agaaaattta ttcaccaggg aaaaatattc
     4321 gcgaagcaga gtttcagcaa tttttaaaaa tacggtggga gaatgaatat gattactgac
     4381 aaaaaataaa ttgtttaata aaagttaaaa tgtatgacat ttaaaatatc tttgtttaaa
     4441 tccgtttaaa agaaaaataa ccaacatcat ttcatggaaa atacttcaca ctcttttttc
     4501 gaccgactca atcaatggat aaaaaattct gtcatggtga aactcctcag catggcaatt
     4561 ttgattttat tattacttat accatcagaa atggtgcaaa gtctgattaa cgaaagacaa
     4621 caaacacatg acagtgcatt agacgaaatt tcatcaaagt ggggatttga gcagcacctc
     4681 agcgggcctg tgattaatgt accttaccaa agtttttttg ttgatgccga tggaaaaaga
     4741 aaatctcaaa ttcaatatgc acattttttg cctgaagaat taaaaattgc aggaacgctt
     4801 gagcctgaaa tgcgtcatcg cggcatttac gaagctgtag tgtataagtc agatgtttcg
     4861 ttaaaaggat attttcgttt tcctgatttt tctgaatgga atattgcccc atcagatatt
     4921 atgtggaaag agactacgct gtctttaggc atttctgatt tgcgcggaat caaacaacgt
     4981 ctaaccatca acctgaatgg aaattctatt gagtttaatc cgggagtaga aaataatgat
     5041 gtgcatgaat cgggtgtaag catacgttta ccaatagccg attcgctcaa taaaccggat
     5101 tttaaatttg atatgtcttt taagctcaac ggcagcaagg aaatgtattt tatgcctttg
     5161 ggaaaagaga ctactgttaa cattaaatcg gactggacta atccgagttt tgatggcgca
     5221 tttctgcccg acacacatca catcagcgat aaaggctttg atgcagagtg gaaaatattt
     5281 caccttaaca gaaactacgg acaaaaattc agaggagcgc caacaggatt gcaagaatct
     5341 tcttttggtg ttaagctgct tattcctgtt gacaattatc agaaaactta ccgctctgcc
     5401 aagtatgcta tattgctaat tactttatcg tttacacttt tcttctttat tgaagtgcta
     5461 aacagaaaac gtattcatcc gtttcagtac attttagttg gtgttgcttt atgtttgttt
     5521 tatactttgt tgttgtctgt atcggaatac atcagtttca atttatcata tctgttatct
     5581 gctgctgcta ttattttact catcagccta tatgcaaaaa caatattcaa caacctgcgc
     5641 ttagctctgt tgtttagtct ggtgctgata attctgtatg tatttgtgtt ttcaatcatt
     5701 cagttacagg actatgcatt gctgctcgga agtataggtt tgtttatcgt gcttgcattg
     5761 gtaatgtact tgtcgcggaa aatcaactgg tatggttcag aaaaataagg gagatcatta
     5821 tcagcttggt cgcgaaggcg aaaacatggc agttgaatat ttaattgcca atggctataa
     5881 gattgtagaa cgcaactggc gttttggaaa attggagatt gatattatag cctctaaagg
     5941 caacctgttg tgcattgtcg aagtaaagac tagaacggga gattattttg gagagcccga
     6001 agaaggtgtt accaaagcta aagaacgttt tctggcaacg gctgccgacc actacatcat
     6061 ttcaaaaaat ttagatgtgg aggtgcgcta tgatattgtt tccattgtaa tttacaatgg
     6121 ccgtcatgaa ttgaaattta ttgaagatgc tttttatcct tactcctgaa ttattgcaga
     6181 agtgtaattt tatttcgtcc cagttccacg cgaccatcaa cctctagttg tttcaacaaa
     6241 cgcgaaacaa ctacacgtgc agtgcccaac tcattggcta attgttcatg agtaacggta
     6301 atggtttttg acttagaaag ttcaactttt ttctgaagta ataaaatcaa gcgttcatca
     6361 atttttttga aagctactgc attcactact tcaagaagct cttcaaagcg tttgtgatac
     6421 agtctgaaaa taaagtcaag ccagtcgtca tgttcgcgaa tgagtgtgat taccttgtca
     6481 acgggaacaa ataaaatttc tgtttcatct tcggcaacag ctctcacctt gcttgtgtcc
     6541 tgatgtatgc cacccagaaa agacatgatg caactttcgc cacttttaat atagtaaagt
     6601 aaaatctctt ttccttcgtc atcattacgc atcactctga tggagccgct gagaacaata
     6661 ggaatagaac ggatgtaggc attttcgttt aatattacat ctccggcatt aaatttttta
     6721 gttgttccat acgaaattag tttttcgcga agtgcgggtg acgatttaaa ttcaagcgat
     6781 tcagtaatat tttccataca gcaaaagtaa acaatgttgt tgcaacaatt cagccttcaa
     6841 taaacaagta taaaccaatc agaacaacca acactgcgcc tggtgtgagt ccatagagtg
     6901 caaaatcgaa taaaaaaggt attggattaa ctatagaaag taaacagcct ataaaaccca
     6961 taaaggcacc tgcagccatc agataaatac ccttggtttg gcgttttata caacacaact
     7021 ttttaaacaa ctctgacgat tttgtatcgg catcagcaat attgtgtgat gaaagcacct
     7081 tttttacctc atcggcagaa gtgcaatgtg taatgagtcg gagaaaatca ttatctgtgt
     7141 aaagaggttg gctgcttgtc atattt
//
LOCUS       DAOOOI010000094         7154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2054_length_7154_cov_37.076067, whole genome shotgun sequence.
ACCESSION   DAOOOI010000094 DAOOOI010000000
VERSION     DAOOOI010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7154
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2054_length_7154_cov_37.076067"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1044
                     /gene="trxB"
                     /locus_tag="PKX59_09505"
     CDS             100..1044
                     /gene="trxB"
                     /locus_tag="PKX59_09505"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOZ91141.1"
                     /translation="MSENSEHVKCLIIGSGPAGYTAAIYAARADLKPVMYQGLQPGGQ
                     LTTTTEVENFPGYPKGTMGPEMMEEFRQQAERFGTDIRWGYASSVDFKGPVHKVIIDE
                     EKEITADTVIIATGASAKWLGLESEQRLNGFGVSACAVCDGYFFRNQHVAIVGAGDTA
                     AEEATYLAKLCTKVYMIVRRDEMRASKAMQHRVMNTPNIEVLFNTETKEVVGEKTVSA
                     VKVVNNITGAERILDVTGFFVAIGHQPNTDIFKGWLDMDKTGYIITKPGSTKTNIEGV
                     FACGDAQDKIYRQAVTAAGTGCMSALDAERYLADKGIH"
     gene            1132..1773
                     /locus_tag="PKX59_09510"
     CDS             1132..1773
                     /locus_tag="PKX59_09510"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOZ91142.1"
                     /translation="MTDTFKHQGLRRQLITELKSKGINDAAVIAAMTKIPRHAFMDNA
                     FIESAYIDKAFPIGSGQTISQPYTVAFQTLLLGIQKGDKVLEIGTGSGYQTAILCELG
                     AKVFSIERHKLLADKAKSLLEKLGYIPKIHFGDGFKGLPAFAPFDKIIVTCGAPDIPQ
                     SLFEQLKTGGVMVVPVGEDVQVMKTVVKKPDQTMEVSDFNSFRFVPMLGNKVR"
     gene            1957..2502
                     /locus_tag="PKX59_09515"
     CDS             1957..2502
                     /locus_tag="PKX59_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOZ91143.1"
                     /translation="MTDRELIEGCIRGDRNCQKALFEKFAGKMKVVCMRYVNNHEDAE
                     DILQEGFIKVFKYISKFGFEGSFEGWIRKIMVNTALTKITRYSYKEEVALVHSNMPVY
                     DYGDVIAEMSAKELKAFINALPDGYRLVFNMYVIEGFSHKEIAESLSISEATSRSQLL
                     KARLALQEKLKKNKIAYSHAG"
     gene            2492..3811
                     /locus_tag="PKX59_09520"
     CDS             2492..3811
                     /locus_tag="PKX59_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91144.1"
                     /translation="MQDEQFDDLIRQKLNSEEESVSAGLWSRVEADLPVAKQGVFNRS
                     LLWLLLLFLIPAGAGWYYFTSNQTKISSLEGNRSSKQKVISNIASEVRNTDDKTMQNN
                     SVQSSDHNTLNNSIESNSSQIKYSSNANSTISANNNTVSKTLEPTYAISSTTNFNNQV
                     ENSEWDLLNPIQASLPEAASSQALISDKPTLEIKKTKRGIRVFADIYYAPEFAQHTRD
                     LGSSSPAYLKLRRNTETNIYSHSQGIRAGVWLSTNFFFKTGIDMFDMNERLSYTFIPG
                     MGNLPVGMRIDSINGYYINPLTNALVADTIVGSVNSTGEVIISNHYRFVNVPLMFGWR
                     FNKGLWSIQSSTGIYMNIYSGYKGKIIHESMQSLIDISSTSTPYKQNIGIGFSAEVIA
                     TYSIFKNIDVLIGPKYNYHLSDISRKGAPIQEKLSSIGMFSGIRYNF"
     gene            complement(3882..4466)
                     /locus_tag="PKX59_09525"
     CDS             complement(3882..4466)
                     /locus_tag="PKX59_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOZ91145.1"
                     /translation="MKKLFLSAIAVTMAFSLNAQTQWSADKSHSKIGFTVTHMLIAEV
                     EGNFKDFTASMTSTKDNFEDSKVEFKAPVSSINTNDDKRDEHLRGSDFFDMANHPEIS
                     FTSVTFKPAGDKKFKVVGNLTMRGVTRSVTLEATHIGTVEDPWGNTKAGFKLTGKVNR
                     KDYGIAWNKTLTSGGAVVSDEVAMNCMIELIKQK"
     gene            complement(4794..5366)
                     /locus_tag="PKX59_09530"
     CDS             complement(4794..5366)
                     /locus_tag="PKX59_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91146.1"
                     /translation="MFIDFGAGQVRLLGNRRYEIIYNMQLHVVDDIVSETSKMEARAG
                     AGVATPEYAVDQALARLDLIDHILYHLTGFTTTGMRGMQCTGVELSSVESSPTHDILT
                     WAVRLQTDTAKKVMIEVEPEVVVIPNSHFPPNPTYSQSQTQLRLQSIPFGEFALLLNC
                     LAQVYFSVWLIFRVYVFLQTFLVVSLIPDR"
     gene            complement(5665..5847)
                     /locus_tag="PKX59_09535"
     CDS             complement(5665..5847)
                     /locus_tag="PKX59_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91147.1"
                     /translation="MQQKNKNPWRNILRGYYEMRKTLADDASRMALNEFLENFQRQGS
                     VNANGLFIPWKATGGG"
     gene            complement(5956..6219)
                     /locus_tag="PKX59_09540"
     CDS             complement(5956..6219)
                     /locus_tag="PKX59_09540"
                     /inference="COORDINATES: protein motif:HMM:NF037113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR2 nuclease fold domain-containing protein"
                     /protein_id="HOZ91148.1"
                     /translation="MRDKIFINVGDVIKNPDEVWMTYYKNKSAQYRYVKFYGDKKTMI
                     VPVDVNMGMDENNKDNLLKIKTWFLLDKEEEKNKRVGVLIYKK"
BASE COUNT         2230 a         1242 c         1354 g         2328 t
ORIGIN      
        1 aatagataaa ttcagatcac agataacaca gttagaaaca gaaattgaca atgctaacca
       61 gagccttaaa gtagttgagg catttaatta aaatatgcaa tgagtgaaaa tagtgaacat
      121 gtaaagtgcc tgattatagg ctcgggtcct gcaggatata cagcagcaat ttatgcagca
      181 agggcagatt taaaacctgt aatgtatcag ggattgcaac ctggaggtca gttgacaaca
      241 acaacagaag tagaaaattt cccgggctac cctaaaggta caatggggcc ggaaatgatg
      301 gaagaatttc gtcagcaggc agagcgtttt ggcaccgaca tcagatgggg ttatgcatca
      361 tcggttgatt ttaagggacc tgtacataaa gtaatcattg acgaagaaaa ggaaattaca
      421 gccgacacag taattattgc aacaggtgca tcagctaagt ggcttggatt agagagcgaa
      481 caacgactga atggttttgg tgtttctgca tgtgccgttt gtgatggata ttttttccgt
      541 aatcagcatg tagcaattgt aggggcaggt gatactgctg ccgaagaagc aacatatctg
      601 gcaaagttgt gcacaaaagt ttatatgatt gtgcgcagag atgaaatgcg agcatcaaaa
      661 gcaatgcaac acagagtaat gaacacacca aatattgaag tcttgtttaa tacagaaact
      721 aaggaggtgg taggagagaa aacagtgtct gctgttaaag ttgttaataa tataaccggt
      781 gcagaacgca tacttgatgt aacaggcttt tttgtggcaa ttgggcatca accaaatacc
      841 gatattttta aagggtggct tgatatggat aaaaccggtt atatcataac caagccgggc
      901 tcaaccaaaa caaacataga aggtgttttt gcttgtggcg atgcacagga taaaatttac
      961 agacaggctg ttactgctgc aggaacaggt tgtatgtctg ctttagatgc agagcggtat
     1021 ttggcagata agggcattca ttaaaaaaat cattagcatt tttttgttaa aaattttatt
     1081 gattcagcaa taaacattgt tgagtgaatg gtaaatttgt ttgcaatgaa tattacagac
     1141 acttttaagc atcagggatt aagacgacag ctaattacag aattaaagtc taaggggata
     1201 aatgatgcag ccgtaatcgc tgccatgaca aaaattccac gtcatgcatt tatggataat
     1261 gcatttatcg aatctgccta tattgacaaa gcttttccca ttggttcagg tcaaacaatt
     1321 tcacaaccct atacggttgc ttttcaaacc ttgttgcttg gtattcaaaa aggtgataaa
     1381 gtgttggaaa ttggaacagg ctccggctat cagacggcta tactttgtga gttaggtgca
     1441 aaagtatttt ctattgagcg gcataaatta cttgccgata aagcaaaaag tttactcgaa
     1501 aaattgggtt atatccctaa aatacatttt ggcgatggct ttaaaggact gcctgcattt
     1561 gcaccatttg acaagataat tgttacctgt ggcgcacccg atattccaca gtcattgttt
     1621 gagcaattga aaaccggagg agtgatggtt gttcctgtag gtgaagatgt gcaggtaatg
     1681 aaaacggttg ttaaaaaacc tgatcaaacc atggaggttt cagatttcaa ctcattccgc
     1741 tttgttccca tgcttggtaa taaagtaaga taagtacatt atagttttat taaaaaacat
     1801 tcacattttt tgagtaatat ttttctctca cttagattta tggcaagtga aggtggtgtt
     1861 ttgctatgtt ttaattaatt ttaccaaagc gcaaaagtca ggcaacgttt cccggactta
     1921 ttgagtcttt atatatgaaa tgcattcaat actgttatga cagaccgtga actaattgaa
     1981 ggttgtattc gcggtgacag aaattgccag aaagctttat tcgaaaagtt tgccggtaaa
     2041 atgaaagtcg tttgcatgcg ctatgtcaat aatcacgaag atgcagaaga tattttacaa
     2101 gaggggttta taaaagtgtt taagtatata tcgaaatttg gttttgaagg atcttttgaa
     2161 ggttggataa gaaaaataat ggttaataca gcgcttacaa aaattacacg ctacagttat
     2221 aaagaagaag ttgccttggt tcatagcaac atgcctgttt atgattatgg tgatgtgatt
     2281 gcagaaatgt ctgccaaaga gctgaaagca ttcataaatg cattgcctga tggttatagg
     2341 ctggtgttta acatgtatgt gatagaaggt ttttcgcata aagaaatagc tgagagcctg
     2401 agtatttcag aagcaacatc gcgtagtcag ttattgaaag cacgattggc attacaagaa
     2461 aaattaaaaa aaaataaaat agcatacagt catgcaggat gaacaatttg atgatctgat
     2521 aaggcagaaa cttaactccg aagaggagtc agtaagtgcc ggactgtgga gtagggtaga
     2581 agcagattta cctgttgcca aacaaggagt ttttaataga agtttactat ggttgctact
     2641 tttattttta attccagcag gtgcaggatg gtattatttt acttcaaatc aaacaaaaat
     2701 ttcttcatta gaaggtaatc gttcaagcaa acaaaaggtg attagtaaca tagcatctga
     2761 ggtaaggaat acagacgaca aaacgatgca aaacaattct gttcagtcat cagatcataa
     2821 tactttaaac aattcaatag aaagtaattc gagtcaaatt aaatatagct ctaacgcaaa
     2881 tagcactatc tcagctaata ataatactgt ctcaaaaact ttagaaccaa cctatgcaat
     2941 ttcatcaaca acaaatttca ataatcaggt tgaaaatagt gagtgggatt tgcttaatcc
     3001 aatacaggca tcattgccgg aggcagcaag tagtcaggct cttatttcag acaaacctac
     3061 tttagaaata aaaaagacaa agagaggaat tcgtgtattt gcagatattt attatgcacc
     3121 tgagtttgcc caacatacac gcgacttagg aagcagcagc cctgcctacc ttaagttgag
     3181 gcgcaataca gaaacaaaca tttattctca cagtcaggga attagggcag gtgtttggtt
     3241 gagtaccaat ttctttttta agacaggaat tgacatgttt gatatgaacg agcgactgag
     3301 ctatacattt attccgggca tgggaaatct ccctgtagga atgagaattg attctatcaa
     3361 tggttattac atcaatccac tcacaaacgc tttagttgct gatacgattg taggttcagt
     3421 aaattctaca ggagaagtca tcatttcaaa tcattatcgt tttgtcaatg tacctttaat
     3481 gtttggatgg cgatttaaca aaggtttatg gagcattcag agcagcacgg gaatttatat
     3541 gaatatttat tcagggtata aaggaaaaat catacatgaa agcatgcaat cgctaattga
     3601 tatttcttcc acctcaacac cttataaaca aaatattggt ataggttttt ctgcagaagt
     3661 aattgcaaca tacagtatct ttaaaaacat tgacgtgctc attggtccta aatataatta
     3721 tcatttaagc gacatcagtc gcaaaggtgc acccattcaa gaaaagttga gtagcatagg
     3781 tatgttttca ggtatcagat ataatttttg atttaggttt ggattaattg aataaaaaag
     3841 gcagcctttt cagaggctgc ctgcaaaaaa ttaattcaca tttatttttg tttgatcagt
     3901 tctatcatac aattcatagc tacttcgtca ctcaccacag caccacctga tgttaaagtc
     3961 ttgttccagg ctataccata atctttacgg ttaacttttc cggttaattt aaagccggct
     4021 tttgtgtttc cccatggatc ttcaacagta cctatatgcg ttgcttctaa ggttacactt
     4081 cttgttacac cacgcatggt aaggttacca acaactttga attttttatc acctgcaggt
     4141 ttaaaagtta cactggtaaa agaaatttct ggatgatttg ccatatcaaa aaagtctgac
     4201 ccacgtagat gctcatcacg tttatcgtca ttggtattga tagacgaaac aggtgcttta
     4261 aactcaactt tgctatcttc aaaattatct tttgtagagg tcattgaagc cgtgaagtct
     4321 ttgaaatttc cttctacttc agcaataagc atgtgtgtaa cggtaaaacc gattttagaa
     4381 tgacttttgt ctgccgacca ttgtgtttgt gcattaaggc tgaaagccat agttactgca
     4441 attgcactta gaaataattt tttcattttt tatatttttt gatttatggt gcaatgatag
     4501 cccaacgata aacattaaat attaacctag attaacttca aaaaaattaa ttaaaatttc
     4561 agttttatat ttgtaacaac atataagata ttatgcagtt gcaacaatgt cataaaaata
     4621 ttactattga attattaatc cgaaagatgt gataaagttt tttctaaaag catggttagt
     4681 gttagttttg atttgtattg tcattctctt ttatgatgac tataaaaaag aaaaacagag
     4741 agagtatgaa aggtcattac ctaaacaacc tgaatgggat acagcaacat cacctaccta
     4801 tcaggaatta atgagacaac taagaaagtt tgaagaaaga cataaacacg aaaaattaac
     4861 caaacagaaa aatatacctg tgcaagacag tttaacaaaa gagcaaattc gccaaaggga
     4921 atggattgaa gacgaagttg agtctgagat tgagaatagg ttggattagg aggaaagtga
     4981 ctattcggaa taaccacaac ttccggctct acttcaatca ttaccttctt ggcagtgtca
     5041 gtctgcaagc gtacagccca tgttagtata tcatgtgtgg ggctgctttc caccgaactt
     5101 aattcaacac ctgtgcattg catgcctctc atgcctgttg tcgtaaatcc tgtaaggtga
     5161 tataatatgt ggtcaatcag gtcgaggcga gccaatgcct ggtcaactgc atattcaggt
     5221 gtggcaacac cggcaccggc acgcgcttcc atcttgcttg tctcactcac aatatcatcc
     5281 accacatgca attgcatatt atatataatc tcatatcgtc tgttgccgag caatcgaact
     5341 tgacctgcac caaaatcaat gaacactgcc ttacgtggga atggtaatga gttggcattt
     5401 tatttgtata tttgttattc aattaagcgg ttaatcttgc aaggggtgag gggctttaaa
     5461 tgtagttcca tcccgcttca caatagtgag attaaccgtt tattttttta taaataagta
     5521 ttcccttcct tacattgccc tcatctttat ctaacttgta ccacgacttt atattaagtc
     5581 ttccgtccac ttcaaccggc accggcatca ttgtagaatt taacgtatgt gtattttgcc
     5641 gccaaaaatg gctgatgctt tgccttagcc accaccggtt gccttccaag gtataaataa
     5701 gccattagca ttaacagacc cttgtctttg gaaattttcc aaaaattcat ttagtgccat
     5761 tcgactggca tcatcggcca gtgttttgcg catttcataa tatcctcgta atatattgcg
     5821 ccacggattt ttattttttt gttgcattgg tatgattttt attttatatt tgtatccgaa
     5881 acagcaaaat ctgttgcttt ggtctgatgt tggcgtaggt aacgcgaggg atgatcatca
     5941 acagatttag ctgtttcact ttttatatat taatactcct accctcttat ttttttcctc
     6001 ctctttatct aaaagaaacc atgtttttat tttcaataag ttatccttgt tgttctcgtc
     6061 cattcccata ttaacatcta ccggaacaat cattgttttt ttatcgccat aaaatttaac
     6121 atatcggtac tgagcggatt tatttttata gtaggtcatc caaacctcgt ctgggttttt
     6181 gataacatca cctacgttta taaatatttt atcgcgcatt tccttatcac ttaattattt
     6241 attgcctttc gcatggggcg ttatcatttg ctttattcgc aagtgttttt attgtttaag
     6301 gcgctcatga acctcgttcc tcggtttcaa cttttaggat ttagaacttt caaaccttca
     6361 aaccttcaaa cattgtaaca ttcaaaactc aggtgttgtg atttgcttta ttcgcaagtg
     6421 tttttatttt ttttaaggcg ctcatgaacc tcgttcctcg gtttcaactt ttaggatata
     6481 caactttcaa accttcaaac tttgtaacat tcaaaactca ggtgttgtga tttgctttat
     6541 tcgcaagtgt ttttattttt tttaaggcgc tcatgaacct cgttcctcgg tttcaacttt
     6601 taggatatac aactttcaaa ccttcaaact ttgtaacatt caaaactcag gtgttgtgat
     6661 ttgctttcaa cttttatctt tgtcagagtt ctttcaatca attcattgca gtgatgcaga
     6721 gattcaacgt tgtgatttgc tttcaacttt tatctttgtc agagttcttt caatttctga
     6781 taattaatcc accgctacct gcaagttgtg atttgctttc aacttttatc tttgtcagag
     6841 ttctttcaat attcaaaacg gatcaggcag aggccaaata gttgtgattt gctttcaact
     6901 tttatctttg tcagagttct ttcaattaag acagtgcaat gaatacaagc agcacgttgt
     6961 gatttgcttt caacttttat ctttgtcaga gttctttcaa tatgttgaga aaatgccttt
     7021 cctcaatgcc agttgtgatt tgctttcaac ttttatcttt gtcagagttc tttcaattac
     7081 ttctttagct tctactgaat taaagccgtt gtgatttgct ttcaactttt atctttgtca
     7141 gagttctttc aatt
//
LOCUS       DAOOOI010000095         7134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2064_length_7134_cov_10.895607, whole genome shotgun sequence.
ACCESSION   DAOOOI010000095 DAOOOI010000000
VERSION     DAOOOI010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7134
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2064_length_7134_cov_10.895607"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..5578
                     /locus_tag="PKX59_09545"
     CDS             41..5578
                     /locus_tag="PKX59_09545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91149.1"
                     /translation="MQACCRIDKGATPYQINFYTLPNPSNCFTLTQTTPNTLPVISDV
                     RQQWLPLSYTGDQISVHCPGCLAPGAIVSNYKINRVSFGLQDSNNDGRTDNATKIIKG
                     DNYYNTFKNYLNENFAHYSDTLEDYLSAYFQDGDNSGTGGGYTYQQLVTAGVPLHFMQ
                     LSRIIPFGLDSFNLTIDSVVLYIDTLIPNTNACIDCADFNLTGNGVVTQSVVAFNRVD
                     AYSHCVMADVPRNQLLFTFCDTTGGNIQSFETFTNTSFPFVAFKTGQRYRLKVRYSVC
                     GNYRLPFNSVSNLVCNSPISNNLWFTGKEQGADAIATLPQMPNTVGAMETDFHYSFNA
                     SSGYPLPNQNFVDSARFYCETFGGRFYFAPTSYYNNSTVTNSQGCEKRITAVYIADLA
                     GYRNRIFDVYPYEYRVPPLFADTINIQVPNGYYISMMRARSSVYYTNNGQPMAPFSAY
                     DTLTAPQLTGLVQIPIDSLPVMHCLQQSMSPAGNDTAAFAGDQFTRLELQCFIRRFDC
                     STAIDTLPLSSVYTGTGLFQSECNPTENLSCTPLAIAADSIPFNANIQPLLVHPNLNL
                     VLPVSHVANAQQQQLCWTVKVLNPKVVVQPGTPDIYSTGADNVFLGVPLNGAIPALQN
                     WQFTPTGQSTINAVNGIIPLTTHLSPNDSVTGQLCATFVSCDSTIKTFPLYYGWNCND
                     FPTATSQIPDTVCGADYVTLGIEYFEASLTGEGKGYYPNNYTLCDTLKKFTCIVNSNP
                     GYAYPDFAGLINIPPGLNILSAYILNPNATPVAVSYLTGSAPSWQITPANMQALGFAN
                     GGIHLTDKLCIYFNLIPECAFAADTLLPDILVAGKNYCGDSLSYTAYFSYNNQSPLFM
                     DSTACTDCWSITKTADTDTAAAVTDTVTYTITVCNNSANTQTGTLADNTPANFVITNS
                     TLPATVTLNSMQCDTFTVSGYFTSPGSCFYNVASVTSPMGTTWKDSVCVTVINVCNVP
                     NAQQIADSSFSLPISASYSNASFVLHGRFYVNDTLTLINCSVYAYPGAQIIVLTGGTL
                     ILQGTSITACTQMWKGIVLNDKSRLVMTEQSLVADADIGIQAMNGSSFFLLGSSVTDC
                     VRSIFVPQQSNGMNNIQGYVNDCRFGLYAAAFKPDYTGQPPHHTMPRAGMEFNDVVIT
                     IGDYEYNEFRNCNWGIYAVRSMLKVQKGRFYNMNKGGSIYGSAAHKGSAIVAESKTAA
                     KAGKIQVADCQIDTCEHGIYTEWTTADINNVNALHVTGNGSYHLYCNSANMSTYVGAC
                     NIVAGKAGIVFQNSEQATMTAKNNTLKVTTGGSSVGIKVIATNNNTGNYMLKGNVIEA
                     ANGNGITVNFAKNATIVDNMIKLSGNTTNGIALTGCDSSTVSCNTVSGRYPAMGYQNK
                     GVNISHSTNNFMSCNFTDSTYFGNYFEGACNGTRFRGTSINRHFEGLRLYTNAVIDTQ
                     AHAGNLWFGPFTTGGYGANNLNNGSQIALDQSALLIDYSYGGAYLPTIPVNNTGWIIN
                     DVGTEFNCSGYILCEDISHDERAASQLQLTIAEDSLKTSEFTDETKIMAKNYLYKDLK
                     ENDSLANGNFLLTSFLVANENTATGHLYDVNNAIKEADIIGGAESQTLSILSSLTDSV
                     IGNIYYIDSLANADSTLNLINQRDSLVQQLIIALQNKTDLLNQLGQAKEQSLINTQSV
                     SNSIITSNTPDEYEKEMCDVEIAYQTGGKAELQSRYSQVLNIAIQCPHVGGKAVYKAR
                     SFMALLNDSIEYDDVSVCTQAGYRKAATNEPANEVIKGNISIIPNPANEVITVTLSDD
                     ISGICKILFYDVVGKLILSKELDCNKKTHSLSVKNLSEGVYTVKAKIANQNSKQFKLI
                     VVR"
     gene            5608..7104
                     /locus_tag="PKX59_09550"
     CDS             5608..7104
                     /locus_tag="PKX59_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91150.1"
                     /translation="MILLQIILFANSCLSQGYNHTWLLGYHPNVNNPFDTMAQIDFNI
                     SSFSIVNYQRKIPFRGFTQANISDANGNLLMSSNGAWIANSTGDTMMNGSFLGYDSYI
                     NANAQGLNITYAAIFIPYPDDSTKYVLFHQSLDSDGISYYSPAINYSIIDITKDGGLG
                     AVDSTQKYIAAFQDTMNGGIAACRHANGRDWWVVAQKHDTDTIYKILFTPTGIASITT
                     QKLNVPKAWYSVPHLTFSPDGKKFVYQTYIPNQPDSSYILTFDFDRCSGLFTTHNPVF
                     VNRGYIWGLAFSPNSQFVYACTADKLYQLNLNTYNLDTIATYDGFNSPYTWCCPTTFF
                     NCYLAANGKIYITSGNAIQHIHEINYPDSTGTACDFQQHAIPIDCYHRGSVPNHPNYY
                     LGCDTTQTTCPCLITGINEIKQHNFKFSVTPNPTNGNFKIVYLLPQDKSGTLQIFDIT
                     GKEVYKQNLPPWSTMQYISLPKLANGVYYCTITSNNERVHKKLVVFKE"
BASE COUNT         2181 a         1575 c         1431 g         1947 t
ORIGIN      
        1 ctttaaagca tttttagaca gtgcccattt tgaatttacc gtgcaggcct gctgcagaat
       61 agataaagga gcaacaccct atcaaatcaa cttttatacc ttgcccaatc cgtcaaactg
      121 ttttacgctg acacaaacca cgcccaatac cctgcccgtg atttctgatg tgcgtcaaca
      181 atggctgcca ctaagctaca ccggtgacca gataagtgtg cactgcccgg gatgccttgc
      241 cccaggtgct attgtttcaa attataaaat caatcgcgtt tcttttggct tacaggacag
      301 caataatgac ggccgcactg ataatgcaac aaagataatc aaaggcgaca actattacaa
      361 cacttttaaa aattatttaa acgaaaattt tgcccattac agcgatacgc tggaagatta
      421 tctttctgcc tattttcagg atggcgacaa ctccggcacc ggtggcggat acacctatca
      481 gcaattggtg actgccggtg tgccgctgca ctttatgcaa ctgagccgca tcatcccctt
      541 tgggctggat tcttttaacc ttaccattga cagtgtagtg ctttatattg acacgcttat
      601 tcccaacacc aatgcctgca ttgattgtgc agactttaac cttacgggca atggtgtagt
      661 tacccaaagt gtggtggctt ttaatcgtgt cgatgcttac agccattgtg ttatggccga
      721 tgtgccacgc aatcagttgc tgtttacttt ttgcgacact accggtggca atattcaatc
      781 cttcgaaact tttaccaaca cctcttttcc ctttgtggca tttaaaactg ggcaacgcta
      841 caggcttaaa gtgcgctact cggtttgtgg caactaccgt ctgccgttta attctgtaag
      901 caacttagtg tgcaattcgc ccatcagcaa caacctgtgg tttacaggca aagagcaagg
      961 tgccgatgcc attgctaccc tgccacaaat gcccaacact gttggtgcta tggaaacaga
     1021 ctttcattat tcctttaacg caagcagtgg ttatccgttg cctaatcaga actttgttga
     1081 ctctgcacgt ttctactgcg aaacctttgg cggcaggttt tattttgcac ctacctctta
     1141 ttacaacaac agcactgtga ccaacagtca gggttgcgaa aaacgaatca cagccgttta
     1201 tattgccgac cttgcaggct acagaaaccg tatctttgat gtttatccat acgaatatcg
     1261 agtgccgcca ctatttgccg ataccatcaa cattcaggtg cctaacggct attatatttc
     1321 tatgatgaga gcaagatcaa gtgtatatta taccaacaac ggacaaccga tggcgccctt
     1381 ctctgcttac gatacactga cagcgccaca gcttacaggc ttagtgcaga tacccattga
     1441 ctcactgccc gtcatgcatt gtttgcaaca aagcatgagt cctgcaggca atgatacggc
     1501 agcttttgct ggcgaccagt ttacccgatt ggagttgcag tgctttatcc gcaggttcga
     1561 ctgcagcacg gccattgaca ccctgccgct gtcttccgtt tataccggca caggattgtt
     1621 tcagtcggaa tgcaatccaa cagaaaacct ctcttgcaca cctttagcaa tagcagcaga
     1681 ctccattccg tttaatgcca acatacagcc gctattggta catcccaacc tgaaccttgt
     1741 attgcctgtg agccatgttg ccaatgcgca gcagcaacag ttgtgctgga cagttaaagt
     1801 gctcaaccca aaggttgttg tacagcccgg aacgcctgac atttacagca ccggtgccga
     1861 caatgtgttt ttgggagtgc ctttaaatgg ggcaatccca gcccttcaaa actggcagtt
     1921 tacacccacg ggccagagta ccatcaatgc cgttaacggc atcattccgc ttacgaccca
     1981 tctgtcacct aatgattctg ttacagggca gctatgcgct acttttgtga gttgcgacag
     2041 caccataaag actttccctt tatattacgg atggaactgc aacgacttcc ctacggccac
     2101 ttcgcaaata cccgatacgg tatgtggtgc cgactatgtg acgcttggaa tagaatattt
     2161 tgaagcatcc ctaacgggcg aaggcaaagg ctattatccc aacaattaca ccctttgcga
     2221 cacccttaaa aaatttacct gcattgttaa cagcaatccg ggatatgcct atcccgactt
     2281 tgcagggctg ataaatatac caccggggct gaacatactc agtgcctata tattgaaccc
     2341 caatgctacg cccgttgcag tctcctacct taccggcagt gcaccctcgt ggcagataac
     2401 ccctgccaac atgcaggcac tgggctttgc caatggcggc atacacctta cagacaagtt
     2461 gtgcatttat tttaatctta ttcctgagtg tgcatttgcc gcagacaccc ttttgcccga
     2521 catacttgta gccggtaaaa actactgtgg cgactcgctt tcctatacag cctactttag
     2581 ctacaacaac caaagtccgc tgtttatgga cagcactgcc tgtacagact gctggagcat
     2641 taccaaaact gccgacaccg acacggcagc agccgttacc gacacggtga cctacaccat
     2701 taccgtatgc aacaacagcg caaatacgca gaccggtacc cttgcagaca acacccctgc
     2761 aaattttgtg attaccaaca gtacactgcc tgcaacagta acgttaaact ccatgcagtg
     2821 tgatacgttt accgtatcag gatattttac ctctccggga agttgttttt acaatgtagc
     2881 ctctgttact tcgccaatgg gcactacgtg gaaggatagt gtttgtgtta cagtgataaa
     2941 tgtatgcaac gtacccaatg cccagcaaat tgcagatagc agcttttcat taccaataag
     3001 tgcaagctac agcaatgcca gtttcgtctt acatggaagg ttttatgtaa acgacacctt
     3061 aaccctcatc aactgctctg tttatgccta tccgggagcg cagataattg ttctcacagg
     3121 tggcacactc atattgcagg gaacatccat caccgcctgc acacagatgt ggaaaggtat
     3181 tgtgctgaac gataaatcca gattggtgat gacagagcaa agccttgttg ccgatgccga
     3241 tataggcata caggccatga atggctcatc attctttctg ctcggcagca gtgttaccga
     3301 ctgtgtgcgc agcatctttg tgccacagca aagcaacgga atgaacaaca tccagggcta
     3361 cgttaacgat tgtcgttttg ggctttatgc agcagccttc aagcccgatt atacagggca
     3421 gcctccacac cataccatgc ctcgagccgg tatggagttt aatgatgtgg taataaccat
     3481 tggcgattac gagtacaatg agtttcgtaa ctgcaactgg ggcatttacg cagtgcgctc
     3541 catgttgaaa gttcagaaag gccgctttta caacatgaat aaagggggca gtatctatgg
     3601 cagtgcagca cataaaggct cggccattgt tgccgagagc aagacggctg ccaaggcagg
     3661 aaaaatacag gttgccgact gccagataga tacctgcgag catggcattt acacagagtg
     3721 gacaacggca gacataaaca acgtaaatgc cctgcacgtt accggcaacg gcagctacca
     3781 tctgtactgc aacagtgcca acatgagcac ctatgttgga gcttgcaata tagtagcggg
     3841 caaagcaggc atcgtatttc aaaacagtga gcaggcaacc atgacggcaa agaacaacac
     3901 gttaaaagta accaccggtg gaagctctgt gggcataaag gttatagcta ctaacaacaa
     3961 tacaggtaac tatatgttaa aaggtaatgt gattgaagca gccaacggca atggcataac
     4021 ggttaacttt gccaaaaacg caaccatcgt tgacaacatg ataaaactta gcggcaacac
     4081 caccaacggc atagccctta caggctgcga cagttcaacg gtgagttgca ataccgtgag
     4141 cgggcgttat cctgcgatgg ggtatcaaaa caaaggtgtt aatatatcac acagcactaa
     4201 taattttatg agttgtaatt ttacagattc aacttatttc gggaattatt ttgaaggggc
     4261 ttgtaatggt acacgattta gaggcacatc cataaacaga cactttgaag gactgaggct
     4321 atacaccaat gctgtaatag atacgcaagc ccatgcagga aacctttggt ttggaccctt
     4381 tacgacaggg ggctatggtg caaataattt gaataatggt tcgcagattg cattagatca
     4441 atctgcactt ttaattgatt acagctatgg tggagcatac ctgccaacta taccagtaaa
     4501 taatacagga tggatcatta atgatgttgg aactgaattt aattgctcag gctatatact
     4561 ttgtgaagat atttctcatg atgaaagggc agcttctcaa ctgcaactta ccattgcgga
     4621 agacagttta aaaacatctg aattcacaga tgaaacgaag ataatggcaa aaaattattt
     4681 gtataaagat ttaaaagaaa acgattcatt ggctaatgga aattttttgc tcacttcatt
     4741 tttggtagca aatgaaaaca cggcaactgg gcatttgtat gatgtcaata atgccataaa
     4801 agaagccgat atcattggtg gagctgaaag tcaaaccttg agcatattga gcagtttaac
     4861 cgattccgtt ataggaaaca tttattatat agacagttta gctaatgccg attcaacatt
     4921 aaatttaatt aatcaaagag atagtttagt gcagcaacta attattgcgc ttcaaaataa
     4981 aacagattta cttaatcaat taggtcaagc aaaagagcaa tcattgataa acacacaatc
     5041 tgtaagtaat tcaatcatta caagtaatac acctgatgaa tacgaaaagg aaatgtgcga
     5101 tgtagagata gcctatcaaa caggaggaaa agcagaattg caaagtaggt atagtcaggt
     5161 tttgaatata gctattcagt gtccgcacgt aggcggaaaa gcggtgtata aggcaagaag
     5221 ttttatggct ctattgaatg attcaattga gtatgatgat gtttctgtat gtacacaagc
     5281 aggatataga aaagcagcta ctaatgaacc tgcaaatgaa gtaataaaag gaaacatctc
     5341 cataattcca aatccagcca atgaggtaat aactgttaca ttgagtgatg atataagtgg
     5401 aatttgtaaa atactgtttt atgatgtagt tggcaaatta attctatcaa aagaattgga
     5461 ttgtaataaa aaaacacatt cactgagtgt taaaaaccta agtgaaggtg tatacactgt
     5521 aaaagcaaaa atagccaatc aaaattcaaa acagtttaag ttgatagtag tacgatgaaa
     5581 cgatttgata tcctaatcag aagcaaaatg attttgctgc aaatcatttt gtttgcaaac
     5641 agttgtttat cgcaaggtta taatcatact tggctcttag gatatcatcc taatgtaaac
     5701 aatccttttg acactatggc acaaattgat tttaacattt caagtttcag tatagtaaat
     5761 tatcaaagaa aaatcccttt cagggggttt acacaggcaa acattagtga tgccaacgga
     5821 aatttattaa tgagcagtaa tggtgcatgg atagccaaca gcactggcga caccatgatg
     5881 aatggaagtt ttttggggta tgattcatat ataaatgcaa atgcacaagg gctgaatatt
     5941 acatatgcag ccatatttat tccttacccc gatgattcaa ccaaatatgt tttatttcat
     6001 caatcattag acagtgatgg tatttcttat tacagtcctg ccataaatta cagcattata
     6061 gatataacaa aagacggtgg tttaggtgca gttgattcaa ctcaaaaata tattgctgct
     6121 tttcaggata ctatgaatgg tggaattgcc gcctgccgcc atgccaatgg tagagactgg
     6181 tgggtggtgg ctcaaaaaca tgatacggat acaatctata aaattctttt tactccaaca
     6241 ggcattgcaa gcattacaac tcaaaagtta aatgttccga aggcatggta cagcgttcct
     6301 cacttaacct tttcgcctga cggtaaaaaa tttgtttacc aaacctatat tccaaatcaa
     6361 ccagacagca gttatatttt aacttttgat tttgaccgat gttcggggtt gtttacaacg
     6421 cacaatcctg tatttgtaaa tagaggatat atctggggct tggcattttc tccaaattca
     6481 cagtttgtat atgcctgcac cgcagataaa ttatatcagt taaatttaaa tacatataat
     6541 ttagatacca tagctaccta tgatggtttt aactcgcctt atacatggtg ctgtccgacc
     6601 actttcttta actgctacct tgctgcaaac ggtaaaattt acattacctc cggaaatgca
     6661 attcaacata tacatgagat aaattatcct gacagtacag gcacagcctg tgactttcaa
     6721 caacacgcta ttcctattga ttgttatcat agaggttctg tacccaatca ccctaattat
     6781 tatttaggct gcgatactac acaaaccact tgcccttgtt tgataacagg cataaatgaa
     6841 attaagcaac acaattttaa gttttctgtt acaccaaacc ctacaaacgg caacttcaaa
     6901 atagtttatc tcttgccaca ggacaaaagt ggtacactcc aaatttttga tattacaggt
     6961 aaagaagttt acaaacaaaa cctgccaccg tggagtacaa tgcaatatat atccttacca
     7021 aaacttgcaa acggagttta ttattgcaca atcacaagca ataatgaaag agtgcataaa
     7081 aagttagttg tctttaagga gtagtaaaaa aaagcttccc catttagaga aagc
//
LOCUS       DAOOOI010000096         6995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2111_length_6995_cov_11.049280, whole genome shotgun sequence.
ACCESSION   DAOOOI010000096 DAOOOI010000000
VERSION     DAOOOI010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2111_length_6995_cov_11.049280"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..2097
                     /gene="ftsH"
                     /locus_tag="PKX59_09555"
     CDS             82..2097
                     /gene="ftsH"
                     /locus_tag="PKX59_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOZ91151.1"
                     /translation="MPKPPGKGFNFSWIYAIVFLLIFVIWVLGLGDKPKLTNMNEFEK
                     TMLATHDVEKIEIVNRERVEITIKKDKLNQEKYKAVSHKPFGNGYNPGPHYYMPIGDV
                     GNFESKLEAIQKDFPENERIQPEYNNNDRSILEVLFWNLLPFIIIAVIWIFIMRRMGG
                     GGAGSQIFNIGKSKAQLFDKDLNVKVTFNDVAGLEEAKVEVMEIVDFLKNPKKYTQLG
                     GKIPRGALLVGPPGTGKTLLAKAVAGEAQVPFFSLSGSDFVEMFVGVGTSRVRDLFRQ
                     AKEKAPSIIFIDEIDAIGRARGKAPSFSANDERESTLNQLLTEMDGFGSNTGVIILAA
                     TNRADILDRALLRPGRFDRQIYVELPDLTGREEIFKVHTKTLKIDHTVDIKFLARQTP
                     GFSGADIANLCNEAALIAARKNKTAIGKDDFLSAVDRIIGGLEKKNKIITKHEKSVIA
                     YHESGHATVSWLLEHAHPLVKVTIVPRGNSLGAAWYLPEERQLTNKEQIFDEICAALG
                     GRAAEEVIFGTISTGALSDLEKITKQAYASVSIFGLNERIGNISYYDSSGQQDYSFSK
                     PYSEKTAEIIDEEVKKLIDLAYQRTKKLLVDNKEKLDKLAHRLLEKEVIFKEDLEEIF
                     GHRPWGESEKATFNSFITDGEPEPEKTVPPAVDSPPPSNIPEGSIQV"
     gene            2101..2754
                     /locus_tag="PKX59_09560"
     CDS             2101..2754
                     /locus_tag="PKX59_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91152.1"
                     /translation="MDESTNREKVLKKVRSALIAKTPNPYPNLDMDKVVYNSTSYSPE
                     IQFAENFTQQGGQFVLCNGRVELMETILGVCDANDFTEVVCVDRNLSAFFDEFEFPHQ
                     SVFSEEDDTDVQVVIIPCECLVVRNGLIVLSEDNIYQKALLRATQHLVIIAHTQQLIP
                     DITEALAYVRSQNDNNLPSELHFATPNFLNQSHTEVETNTLVKNITVILIQPYDSEL"
     gene            2948..4759
                     /locus_tag="PKX59_09565"
     CDS             2948..4759
                     /locus_tag="PKX59_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91153.1"
                     /translation="MSHSDLHRFHIPVMGLAFTVDTPVKVARYGISSVVSIIEHNMLE
                     VMREFYYKQNGKDFTAITKKDEDYRAKRITSYLNIINEIVLKQITDLRNESFETGKEI
                     NKYFEMLPDTSEAKLLYRKMQASTNQVEKEQLQQELRKFIKSGAIDVNIMTKVDRSNY
                     DANGQPLAQEFSDAQTALRGFANSNLSSSMVFSAGLNPRLYSYCEKFDDFFPDETGFV
                     KKKITLKVSDFRSAQVQGRFFAKKGIWISEFRVESGLNCGGHAFATDGLLLGPILEEF
                     KLNKNALYEELFNTCQTALAQKNRPQYKEKPKMKVTVQGGIGTSQENDFLYQHYEVDL
                     TGWGSPFLLVPEATNVDQETLGLLATASKDDYYLSDASPLGVPFNNFRKSSAERQRKE
                     RIAKNRPGSACYKKFLAFNTEYGGEPICTASRKYQHLKLQELKQNNVEPSQKQIDDLL
                     IKDCLCEGLASPVMLVNNVPVPHNLNAVTICPGPNLAYFSGTFTLQEMINHIYGRANV
                     LNSLYRSHMFVNELKLYIDYLKKDIQKNAATLNDKKEKQLQLFKENLSKGIEYYRHLA
                     VKLKQETKQSIENFKNDLMECELNLKMLQINSPVPAL"
     gene            complement(4856..5959)
                     /locus_tag="PKX59_09570"
     CDS             complement(4856..5959)
                     /locus_tag="PKX59_09570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91154.1"
                     /translation="MKQIYTTMIALASAFCAHAQITLTQSDFAGAGDNILVSNADATI
                     PIDFTTTGANQTWDFSSMIAQSQDTLEFLSVASTGGTYSVYFANVGFNSNRSNIAVAA
                     GTLPTVPGLPITLSDPYSFYYKGSGDYKQQGLGITISGFQTPIAYSSKDILYNFPIAF
                     NDVDSCQAAWNFSLSGLGYYGFEQKRVNHVDGWGTVITPYGSFNALRIKTELYAHDTL
                     YADTLGFGYSMDRPKAVEYKWIANNEKIPVLQINTTDVLGTETVASVVYIDSLRSVGI
                     SSTELLSNFSVAPNPAEKYFVISYSLTKPGQVSIKLTDIKGVEVMNEMQGIQSTGSHQ
                     KMIGTQLLGAGVYFLTITTDNFSQTQKVIVRKN"
     gene            complement(5980..>6995)
                     /locus_tag="PKX59_09575"
     CDS             complement(5980..>6995)
                     /locus_tag="PKX59_09575"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HOZ91155.1"
                     /translation="MIKSAGEPFSREPEIAMKRSLLHQSFQGFNVQPEDDYIKSKKPV
                     LVNNDLQIALAAPRKSTKDYFLKNADADELIFVHEGSGTFHSIYGSMDFGYGDYIVIP
                     RGTIFQLHFNTENNRLLVVDSFSAIQTPRRYRNEYGQFEEHSPFCERDFRRPQNLKTF
                     DEKGEFLVMIKKESIVYPFVYATHPFDAIGWDGYCYPYIFSIHDFEPITGRIHLPPPI
                     HQTFEAHNFVVCSFVPRLFDYHPLSIPVPYNHSNIDSDEMLYYVDGDFMSRKNVTKGQ
                     ITLHPAGIPHGPHPGTVEKSLGAKETKELAVMIDTFKPLWLTKEAMNIIIPDYYKSWV
                     ENG"
BASE COUNT         2334 a         1292 c         1308 g         2061 t
ORIGIN      
        1 acaagaaaaa ctaaagattg aataaaaaag atgtcagaag agaataaaag cggagacaag
       61 aagaaatcac catttggcaa aatgccaaaa cctcccggga agggatttaa tttttcatgg
      121 atttatgcca ttgtattcct gttgattttt gtcatttggg tattaggtct tggcgataaa
      181 cccaaactga ccaacatgaa tgagtttgaa aaaaccatgc tagccactca tgatgtagaa
      241 aaaattgaaa tcgttaaccg cgaacgtgta gagataacca ttaaaaaaga caagctaaac
      301 caggagaaat ataaagctgt tagtcacaaa ccttttggca atggctacaa ccccggcccg
      361 cattactaca tgcccattgg cgatgtcggc aactttgaat caaaattaga agccatccag
      421 aaagacttcc ctgagaatga aagaatacaa cctgaataca acaacaacga ccgctctatt
      481 cttgaagtgt tattttggaa tttattgccg tttatcatca ttgccgtaat ctggattttt
      541 atcatgcgca gaatgggtgg cggtggtgcc ggcagtcaga ttttcaacat cggaaaatct
      601 aaggcacagc tatttgataa agacttgaat gtaaaagtca cgtttaatga tgttgccggg
      661 cttgaagaag ccaaggtgga agtaatggag attgttgact tccttaaaaa tcctaaaaaa
      721 tacacacaac ttggaggtaa aattccaaga ggtgccttgt tggttggccc tccgggaacc
      781 ggtaaaacgt tattggcaaa agctgttgcc ggtgaagcac aagtgccttt cttttcactt
      841 tcaggatcag actttgtaga gatgtttgtg ggtgtaggca catcgcgtgt gcgcgattta
      901 ttccgtcagg caaaagaaaa agcaccaagt ataattttta ttgatgagat tgatgcaata
      961 ggtcgcgccc gaggcaaagc cccatctttc agtgctaatg acgaacgcga aagtacactc
     1021 aaccaattat tgacggagat ggatggtttt ggcagcaata ccggagttat tattcttgct
     1081 gcaaccaacc gtgccgatat tttagataga gcattgcttc gcccgggtcg gtttgacaga
     1141 cagatatatg tagaacttcc tgaccttaca ggacgcgaag agatttttaa agtgcataca
     1201 aaaacactga aaattgatca caccgttgat ataaaatttc ttgcccgcca gactccagga
     1261 ttctccggtg ccgatattgc caacctttgt aatgaggctg ctcttattgc tgcacgtaaa
     1321 aataaaacag caattggaaa agatgatttt ctttctgcag ttgacagaat tattggtggt
     1381 cttgaaaaga aaaataaaat catcaccaaa cacgaaaaaa gtgttatagc ctatcatgaa
     1441 tccggacatg caaccgtaag ctggttgttg gaacatgcac atccgctggt aaaagttact
     1501 attgtgccaa gaggtaattc tttaggtgca gcatggtatc tgcccgaaga aaggcagctt
     1561 accaacaaag agcagatttt tgatgagata tgtgcggctt taggtggtcg tgctgcagaa
     1621 gaggtgatat ttggaaccat aagcaccggt gcactttctg atttggaaaa aattaccaaa
     1681 caggcttatg ccagtgtaag catatttggg ctaaacgaga gaatcggaaa catcagctac
     1741 tatgattctt ccggtcagca agactattca ttcagcaagc catacagtga aaaaactgca
     1801 gagattattg atgaagaagt gaaaaagctg attgacttag catatcagcg tacaaaaaaa
     1861 ctgttggttg ataacaaaga aaaattagac aaactagcgc acagactgct tgaaaaagaa
     1921 gtgattttca aagaagattt agaggaaatt tttggtcatc gtccatgggg cgaatcagaa
     1981 aaagccacct tcaattcatt tattacagat ggtgagcctg aaccggagaa aactgttcct
     2041 cctgctgttg acagtccgcc tccatcaaat attccggaag gatctattca ggtataattt
     2101 atggacgaaa gtaccaacag agaaaaggtt ctaaaaaagg tacgtagtgc attaattgca
     2161 aagacgccta atccgtatcc taatctggat atggacaagg ttgtttataa ttccacatcc
     2221 tattcaccgg aaatacagtt tgccgaaaac tttacacaac aaggtggtca gtttgtttta
     2281 tgcaatggtc gcgtagagct aatggaaact atacttggtg tttgcgatgc caatgatttt
     2341 actgaggttg tttgtgttga ccgtaacctt tcagccttct ttgatgaatt tgaatttcca
     2401 catcaaagtg tgttttccga agaagatgat accgatgttc aggtggtgat tattccttgt
     2461 gaatgtttgg ttgtacgtaa tggacttatt gttttatccg aagataacat ttaccaaaag
     2521 gcattacttc gggcaacaca gcatttagtc atcatagcac atacacagca actaattcct
     2581 gatataactg aagcattggc ttatgtacgc tcccaaaatg acaataattt accttctgaa
     2641 ttgcattttg ccaccccaaa ctttttaaat cagagccata ctgaagttga aactaatacc
     2701 cttgtaaaaa acataactgt tattcttatt cagccttacg atagtgagtt atgatacttt
     2761 tatttgaaaa cttcattctt caggtttata aacccaacta tttattcatc aacaatctac
     2821 aattcaagat aaattaaaaa taaatgacaa atatcaatgg taacttcaat tgtattaacg
     2881 taatttgtgt ttcacatatc tttgatatta atcagcccca acattatatc ataaataatt
     2941 aaaatccatg agtcattctg atttacatcg ttttcatatc cccgttatgg gtcttgcctt
     3001 tacggttgac acccctgtca aggtagctcg ttatggaatt tcttcagttg tttccattat
     3061 tgagcataac atgcttgaag ttatgcgtga gttttattac aaacaaaacg gaaaagactt
     3121 tacggcaata actaaaaagg acgaagatta tcgcgcaaaa agaatcacct cttacctcaa
     3181 cataataaac gaaattgttt taaaacaaat tactgattta cgcaatgagt cgtttgaaac
     3241 aggtaaagaa atcaataaat attttgaaat gttacctgat acctctgaag caaagcttct
     3301 ttacaggaag atgcaggcta gcacaaatca ggtagagaaa gaacaattac aacaggagtt
     3361 gcgcaagttt ataaagagtg gtgctattga tgtaaacatc atgactaagg ttgaccgaag
     3421 taactacgat gcaaacggac aacctttagc acaagaattt tctgatgcgc agacagctct
     3481 tcgtggtttt gccaacagca acctttcgtc ttctatggtt ttttctgcag gacttaatcc
     3541 acgtttatat tcctattgcg aaaagtttga tgatttcttt cctgatgaaa caggatttgt
     3601 taaaaagaaa attacactaa aagtaagcga cttcagatcg gcacaagtac aaggacgatt
     3661 ctttgcgaaa aaaggaatat ggatttctga attcagagta gaatcaggac ttaattgcgg
     3721 tggtcatgca tttgctaccg atggattatt attaggtcct attctggaag agtttaagct
     3781 aaacaaaaat gcattgtatg aagagttgtt caacacttgt cagactgctt tggcacagaa
     3841 aaacagacca cagtataagg aaaaacctaa gatgaaagtt actgttcagg ggggaattgg
     3901 tacatctcag gaaaacgact ttctttatca acactatgaa gttgacctta caggatgggg
     3961 aagtccgttt ttattagttc cggaagctac caatgttgat caggaaacat taggattatt
     4021 agcaactgca agtaaagatg attattatct gagtgatgca tcacctcttg gtgtgccgtt
     4081 taacaatttc agaaaatcat ctgctgaaag acagcgcaaa gaacgcattg ctaaaaacag
     4141 acccggtagt gcatgttata aaaaatttct ggcattcaat acagagtatg gtggagaacc
     4201 aatatgtact gcatccagaa agtatcaaca tcttaagcta caggaactaa aacagaataa
     4261 tgtagagcca tcacaaaaac aaattgacga tttgcttatt aaagattgct tatgcgaggg
     4321 attggcttca cctgtaatgc tggtaaataa tgtacccgtt ccacataatc tgaacgcagt
     4381 aactatttgt ccgggaccaa atctggctta tttttcagga acctttaccc tgcaagaaat
     4441 gattaaccat atctatggac gagcaaatgt tttgaactca ttataccgtt cacacatgtt
     4501 tgtaaatgag ctcaaactct atattgatta tcttaaaaaa gacattcaaa aaaatgctgc
     4561 aacactaaac gacaaaaaag aaaagcaatt gcagttattc aaagaaaatc tttcaaaagg
     4621 tattgaatat tacaggcatc ttgctgttaa actcaaacaa gaaacaaaac aatcgataga
     4681 aaactttaaa aacgatttaa tggaatgtga gctaaatcta aagatgcttc aaataaattc
     4741 tcctgtacct gctttgtagg ttcaactaat aataatattt aaatcaaaaa agcagtttca
     4801 ctattaagat gtgaaactgc ttttttgatt tactcattat ctgaataaat aaaatttaat
     4861 tcttcctgac aatcaccttt tgtgtctggc taaaattatc tgttgtaata gtcagaaaat
     4921 aaacacctgc accaagcaat tgtgtaccta tcattttctg atggcttcct gtgctttgaa
     4981 taccttgcat ttcattcata acttcaacgc cttttatatc ggtaagttta atggaaactt
     5041 gtccgggttt agtcagagaa taactaatta caaaatattt ctcggcagga tttggtgcaa
     5101 cagaaaaatt actcaacaat tcagtagaag atatacctac gcttcgcaat gaatcaatat
     5161 acacaacaga tgcaacagtt tctgttccta aaacatcagt agtgtttatc tgtaataccg
     5221 gaattttttc attgttggca atccatttgt actctacagc cttaggcctg tccattgaat
     5281 aaccaaaacc taatgtatcg gcatataatg tatcatgtgc atacaattct gttttaatac
     5341 gtagggcatt gaaacttcca taaggagtaa taactgttcc ccaaccatca acatgattga
     5401 cacgtttttg ttcaaaacca taatatccta atccacttaa agaaaaattc caagctgcct
     5461 gacaggagtc aacatcatta aaagcaatcg gaaaattata caatatatct ttactgctat
     5521 aagcaattgg tgtctgaaat ccgctaatag ttatgcctaa gccttgttgt ttatagtcac
     5581 cgctaccttt gtaataaaaa ctgtatggat cactcaatgt aattggcaat ccgggaaccg
     5641 ttggtaatgt acctgcagcc actgcaatat tagaacggtt actgttaaaa ccaacattag
     5701 caaaatatac agagtatgtt cctcctgttg aagctacact taaaaactca agggtgtcct
     5761 gactttgtgc aatcattgaa ctaaaatccc atgtttgatt tgctcctgtt gtagtaaaat
     5821 caatcggtat ggttgcatca gcattgctga caagaatgtt atcacctgca ccggcaaaat
     5881 cactttgcgt gagggtaatt tgagcatggg cgcaaaaagc cgatgctaag gcaatcatgg
     5941 ttgtgtaaat ttgtttcatt tatatgcttg tttaaattgt tatccgtttt ctacccatga
     6001 tttataataa tcaggaataa taatattcat tgcttctttt gtaagccaaa gaggtttaaa
     6061 agtatctatc atcacagcca actctttagt ttctttagca ccaagtgatt tttcaactgt
     6121 tccgggatgt ggtccgtggg gaataccagc aggatgtaat gtaatttgtc cttttgtaac
     6181 attctttctg ctcataaaat cgccatcaac atagtatagc atttcatcag aatcaatatt
     6241 ggaatgatta taaggaacag gaattgaaag cggatgatag tcaaacaaac gaggtacaaa
     6301 tgagcaaaca acaaaattgt gtgcctcaaa agtctgatgt ataggcggtg gtaaatgtat
     6361 tcttcctgtt ataggttcaa aatcatgaat agaaaaaatg taagggtagc agtatccatc
     6421 ccatccaata gcatcaaacg gatgtgtagc atatacaaaa ggataaacaa tgctctcctt
     6481 tttaatcata acgagaaact ctcccttttc atcaaaggtc ttcagatttt gtggtctgcg
     6541 aaaatcgcgc tcgcaaaaag gactgtgttc ttcaaattgt ccatactcat ttctatatct
     6601 gcgtggagtt tgaatagcac taaaagaatc cactaccagt aatctgttgt tctctgtatt
     6661 aaaatgcagt tgaaaaatag ttcctctcgg aataacaata taatcgccat atccaaagtc
     6721 catgcttcca taaatagagt gaaatgttcc gctaccttca tgcacaaaaa tcaattcatc
     6781 agcatcggca ttttttaaaa aataatcttt agttgactta cggggagcag ctaatgctat
     6841 ctgcaaatcg ttatttacca aaacaggttt cttacttttt atgtaatcat cctcaggctg
     6901 tacattaaag ccctgaaagc tttgatgtaa caacgaacgc ttcattgcaa tttcgggctc
     6961 gcgcgaaaaa ggctctccgg cacttttaat cattg
//
LOCUS       DAOOOI010000097         6933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2139_length_6933_cov_12.382815, whole genome shotgun sequence.
ACCESSION   DAOOOI010000097 DAOOOI010000000
VERSION     DAOOOI010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2139_length_6933_cov_12.382815"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..3264
                     /locus_tag="PKX59_09580"
     CDS             301..3264
                     /locus_tag="PKX59_09580"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOZ91156.1"
                     /translation="MNKRFYLLILVFLFTRSFSARSQSSLSEKSLGIENDFSVEVFNT
                     ESGFPQNSVIDILQSSNGYLWFSTFSGVVRYDGVKFTVFSNNETKGLLSNTVLIIKED
                     KNKNLWLLDDAGHLVKYDGNTFTGFEKSFGNIKISNFCFSEDGTLYVSTTNNEIYTVN
                     GNILDFSTRINQYNIRLITAGKNNSLLIATTGGLFELKNGTIQQIESIPKTEVLSLCY
                     DRDNFLWVGTIAGIYKVSAAKTEIINLPDESEGYSRIFVDRQNRKWIFDGPDGIYLLG
                     TNDFNQISEESGLSSNTVKSVYQDSEDNIWVGTNNAGVNKLTYKIFRTFSVEDGLIAD
                     GVAPIVKSANGAVYVGNNCGGINKIIEGKIIKIPEPKENTCIWSMLEDKDENLWVGTY
                     GGGLFKFKNDKEIFHFNKTNGLSDDVVFALYQDSKGIIWIGTDKSIFTIQHDTLSHFA
                     KDVIKTKVTYFTEDSIGNIWAASNNGLYKINSQKIDLFTTYHGLPSNSIRSLYFDEEK
                     TLWIGTVRGGFSRLKAGKFASFTKIPELSTLDVFCIAEDNQQNLWFTTNKGIYNVKKY
                     DLNNFADQKSATLSFNYYDRKDGLKTTEFNSGFQPNYLKEDPTHFWFPTIKGVAILNT
                     RRIIKNDYIPQIIIESIKADDKEFSPIGNIDLHQKIKTLEINYTAPKFNNPQKVFFQY
                     KLDGVDADWRAATTERTVRYFNLNPGNYTFKIRLYGIFGTEKSLSFYVPMPFYKSNLF
                     LVIVYMTGIALLGLIAYFRIKHIRNQEKKKTELNKRFAEFELKALQAQMNPHFLFNCL
                     NTIKFFITTNNHTAANKYLTKFSKLLRMFLDHSTSESITLEEEINMLRLYIELEQMRF
                     DEGFNFHLQVDEKIDYKNTEIPATLLQPFVENAINHGLVNLNRQGNLTLIFEQKNGCI
                     LGIIDDDGIGRVEAARLKNQSTSQHISRGTQLIDDRIKTLNSIREQHIGIQIIDKEDE
                     QHNSAGTRVIVTIPI"
     gene            3276..4022
                     /locus_tag="PKX59_09585"
     CDS             3276..4022
                     /locus_tag="PKX59_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOZ91157.1"
                     /translation="MLQTLIVDDEKRGRELLKMILATNCPEVKVTGEASNIKEAYQMI
                     LQNEPDLVLLDIEMPGGTGFDLLSKFDEVNFDVIFITAFDQYAIKAIKFSAMDYILKP
                     VDEEELIKAIKRAEENRNRKNNKEKVNTLISNVQKSSNHQKIGLTSGEGLEFIEIKNI
                     LRCEADGKYTSVFLTDGKKLLVSKNLKEFEDLLTEYNFFRIHHSHLVNLDCIKKYQSG
                     RGGYVVMSDGSTITVSQRKKDDFLSSLKKI"
     gene            4101..4562
                     /locus_tag="PKX59_09590"
     CDS             4101..4562
                     /locus_tag="PKX59_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOZ91158.1"
                     /translation="MKHLFLVLCIAATAFVSCKSDQQKKTIEPEMVNNPASAEPENAD
                     TSLLPKFDFESDNHEFGSITEGEIVSYNFKFKNSGKAPLIITQASASCGCTVPEYSKD
                     PIAPGQEGFIKVTFNSEGKHGMTSKTVTLLANTVPNTKVLTISADISKKDK"
     gene            4559..4882
                     /gene="yajC"
                     /locus_tag="PKX59_09595"
     CDS             4559..4882
                     /gene="yajC"
                     /locus_tag="PKX59_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOZ91159.1"
                     /translation="MMYLTILLQSQGNMAMQQFLMLGLIIVIMYFFMLRPQMKKMKVE
                     KQFKESINKGDKVVTIGGVHGKITEVGETFFMIEIDNNVKIKIDKTAVSAEASKQYRK
                     TDDKK"
     gene            4895..5842
                     /locus_tag="PKX59_09600"
     CDS             4895..5842
                     /locus_tag="PKX59_09600"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOZ91160.1"
                     /translation="MNIFKNIRLKGNASLPVFFICVFVSLVFWILYSLNKNYVYPVTY
                     TVTLQNTPFSKQVISEIPQQVNVTLRSRGFELFWYLIRNRERNLTVDLKKYSVNNEKL
                     GISLQSEFRNQNQNRLKEMDVVTTLPDSVWLYLSSKYMKTVPVVTQFTYSIKKNFGLS
                     GKVSIAPDSITISGEKSQLDGVNEIKTVATDYDNLDRDLKATLSLISPAGKVNLSQKE
                     IKIHIPVEEFIEKKIPVVLNYSINGSGKVILIPEKAELIIQVPLKRYEQLSADSFQVT
                     VSAKRYLKEGQLAVELIHKPQGINVSSIVPSTVNYFIER"
     gene            5842..6423
                     /gene="coaE"
                     /locus_tag="PKX59_09605"
     CDS             5842..6423
                     /gene="coaE"
                     /locus_tag="PKX59_09605"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386593.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOZ91161.1"
                     /translation="MKKIGITGGIGSGKTFVANIFKTLGIPVYDADTAAKQLMNHEPL
                     RSQLKQLFGNSIYNEHDELNRPVLASLIFNDEKKLDAVNALVHPAVAGHFSEWCNNQK
                     ADYILKEAAIMFESGANKELDAVICVSAPETVRIERAMKRDNTSAEKIKAIIAKQLNE
                     EDRNKRCNYLIKNDGTEPLLPQVLKIHDLLKAK"
     gene            6559..6894
                     /locus_tag="PKX59_09610"
     CDS             6559..6894
                     /locus_tag="PKX59_09610"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOZ91162.1"
                     /translation="MLHNFDIILKMMKEYKASRMSEGNKVLPNKIRIDNDGITFRIPG
                     VFSGEEKTIPFSRISSVEVNTPLVGFSSITINTTGEGAIVATGFYKEDVLEIKKIVLE
                     KINVFQNKM"
BASE COUNT         2474 a         1184 c         1225 g         2050 t
ORIGIN      
        1 tcagtgactt ctgctacctg acgattccct gtaccgggaa ggttaaatga aactttatcg
       61 cctttcttga tagtcataat ctactgctga aatcttttac gaatgtaaca taagttggca
      121 ataaacaaaa gccggaaagt aacaattgag tattttagcg ataagattaa gttaaaaagc
      181 atgacaatac tttttatatg acacagtttc acattcaatt tttggctatc tattaactgc
      241 aactgttact ttttaccact taataactga tttaatgctt aatttggcag cataatcaat
      301 atgaataaac gcttttattt actaatctta gtttttcttt ttacaagatc tttttctgcc
      361 aggtcgcaat catctttgtc agagaaaagc cttggtattg aaaacgattt ttctgttgaa
      421 gtatttaata ctgaaagcgg tttcccacaa aactctgtaa ttgatattct acaatcttca
      481 aatggctatt tatggttttc tacttttagt ggtgtagttc gttatgacgg agtaaaattt
      541 acagtgttca gcaataatga aactaaagga ttactatcca acactgtatt aattattaaa
      601 gaagataaaa ataaaaacct atggctgctg gacgatgccg gtcatcttgt aaagtatgat
      661 ggaaatactt ttaccggctt tgaaaaatca tttggtaata taaaaatatc taatttctgt
      721 ttttctgaag atggaacact ctatgtttcc acaacaaaca atgaaatcta taccgtaaac
      781 ggcaacattc tcgattttag tacacgcatc aatcaataca acattcgttt aattacagcc
      841 ggtaaaaaca acagtttatt aatagccaca acaggtggtt tatttgagtt gaaaaatgga
      901 accattcaac agatagaaag tattccaaaa accgaagttt taagtctctg ctacgaccgc
      961 gacaattttc tttgggttgg aacaatagcc ggaatttata aagtaagtgc ggcaaaaact
     1021 gaaataatta atcttcctga tgaatccgaa ggttattcaa ggattttcgt tgaccgacag
     1081 aacagaaagt ggatttttga tggcccagat ggtatatatc ttttaggtac aaatgatttt
     1141 aatcagatct ctgaagagtc tggcttatca tccaacactg tcaaatctgt ttatcaggat
     1201 tcagaagaca atatttgggt gggaaccaac aatgcaggag taaacaagct tacttataaa
     1261 atattcagaa cattttctgt tgaagacgga ttgattgctg atggtgttgc gcctattgtt
     1321 aagtcagcca atggtgcagt ttatgttggc aacaattgtg gtggtattaa taaaattatt
     1381 gaaggaaaga ttattaaaat tcctgagccg aaagaaaaca cctgtatttg gtcaatgctt
     1441 gaagataaag atgagaacct gtgggttgga acttatggtg gaggattatt taaatttaaa
     1501 aatgacaaag aaattttcca tttcaacaaa acaaacggac tttctgatga tgttgttttt
     1561 gcattatatc aggacagtaa gggaataatt tggattggaa ccgataaaag tattttcact
     1621 attcagcatg atactttaag tcactttgca aaagatgtta ttaaaaccaa ggtgacatat
     1681 tttaccgaag attcaattgg aaacatctgg gctgcaagta acaatggctt atataaaatc
     1741 aattctcaga aaattgattt atttacaacc tatcatgggt taccatctaa ctctataaga
     1801 tcgctttatt ttgatgagga aaaaacttta tggataggca cagtaagagg tggtttttca
     1861 agattaaaag cagggaaatt tgcgagcttt accaaaatac ctgaactatc aactttagat
     1921 gttttttgca ttgcagaaga caatcagcaa aacctgtggt ttacaacaaa taaaggtatt
     1981 tacaatgtaa aaaaatacga cctcaacaat tttgctgacc agaaatcagc aacactttct
     2041 tttaattatt acgacagaaa agacggatta aaaactacag aatttaattc ggggtttcaa
     2101 cccaattatc tgaaagaaga tcccacacac ttttggtttc caacaataaa gggtgttgca
     2161 attctaaata caagaagaat tatcaagaac gattacatcc cacaaataat aattgaaagc
     2221 attaaagcag atgataaaga attttcacca attgggaata ttgatctgca ccaaaaaata
     2281 aaaacgctgg aaataaatta tacagcacca aaattcaaca accctcaaaa agtatttttt
     2341 caatataagt tagatggtgt tgatgccgac tggagagcag caactacaga acgcacagtg
     2401 cgttatttta atctgaatcc cgggaattat acttttaaaa ttaggttata cggcattttt
     2461 ggtacagaaa aatcattgag tttttatgtg ccaatgccat tttacaaaag taaccttttc
     2521 ttagtcattg tttatatgac aggcattgca ctacttggtt tgatagctta ctttagaatt
     2581 aaacatatac gcaatcagga aaaaaagaaa acagaactta acaaacgctt tgctgaattt
     2641 gaattaaaag cacttcaggc acaaatgaat ccgcattttt tattcaactg tctcaataca
     2701 attaaatttt ttattacaac caacaaccat acagcagcca ataaatactt aacaaaattc
     2761 tcgaagttgt tacgtatgtt tctcgatcat tccacttccg agagtatcac acttgaagaa
     2821 gaaataaata tgctcagact ttatattgag ctggaacaaa tgcgttttga cgaaggtttt
     2881 aacttccatt tacaggtgga tgagaaaatt gattataaaa acactgagat accggctacc
     2941 ttacttcagc cctttgttga aaacgccatt aaccatggat tggtgaattt aaaccgacaa
     3001 ggcaatttaa cgcttatctt tgagcaaaaa aacggatgta ttcttggtat aattgacgat
     3061 gatggcattg gcagagttga agcagcgcga ttaaaaaacc agtcaacatc acaacatata
     3121 tcaagaggaa cacaattgat tgacgacaga ataaaaacat taaacagcat acgcgaacag
     3181 cacattggca ttcagattat agacaaagaa gacgaacaac ataattcagc cggaacacgt
     3241 gtgattgtta ccataccgat ttaatacaaa acaagatgtt acagacatta atagtagatg
     3301 atgaaaaacg cggccgcgag ctgcttaaaa tgattcttgc caccaattgc cccgaggtaa
     3361 aagttaccgg tgaagcatca aatataaaag aagcctatca gatgattctt cagaatgaac
     3421 ccgatctggt tttattggat attgaaatgc ctggtggcac agggttcgac ctgctctcta
     3481 agtttgatga ggtaaacttt gatgtcattt ttatcacagc attcgatcaa tatgccatta
     3541 aagcaataaa attttctgca atggattata ttctgaaacc cgttgacgaa gaagaattga
     3601 ttaaagccat taaacgtgct gaagaaaacc gaaaccgtaa aaacaataaa gaaaaagtaa
     3661 acaccctgat ttcaaatgtt cagaaaagca gcaatcatca gaaaatcgga ctgacatcgg
     3721 gtgaaggact tgagtttatt gaaataaaaa atattttacg ttgcgaagct gatggaaaat
     3781 atacttccgt ttttcttact gatggcaaaa aactattggt aagtaaaaac ttaaaagagt
     3841 ttgaagacct actcacagaa tacaactttt tcagaattca ccactcacat ttagtaaacc
     3901 tcgactgtat taaaaaatac caaagtggcc gtggcggcta tgtggtgatg agtgatggca
     3961 gcaccattac tgtttctcaa cgaaaaaagg acgacttctt aagcagtctg aagaaaatct
     4021 gataaataag cttttgtcga aaatactgtt tgctgcttag gtaacttagc cttactttcg
     4081 cagcaaaatt gagttatagt atgaaacacc tctttttagt tttatgtatt gctgccactg
     4141 catttgtatc atgcaagtcc gatcagcaaa agaaaaccat agaacctgaa atggtaaaca
     4201 accctgcaag tgcagagcct gaaaatgcag acacttctct tttacccaaa tttgattttg
     4261 aaagcgacaa tcacgaattt ggttctatta ccgaaggcga aattgtatcc tacaatttta
     4321 aatttaaaaa ttccggtaaa gctccgttaa ttatcacaca ggcaagtgcc agctgtggct
     4381 gcaccgttcc tgaatactca aaagacccta ttgctccagg gcaggaagga tttataaaag
     4441 ttacctttaa tagtgaaggt aaacatggca tgacaagtaa gacagtaacc ttacttgcca
     4501 acaccgttcc aaacacaaaa gttttaacca ttagtgcaga tatttctaaa aaagacaaat
     4561 gatgtattta accattctac ttcagtcgca gggcaacatg gccatgcagc aattcctcat
     4621 gttaggatta attattgtaa tcatgtattt ctttatgctg cgaccacaga tgaaaaaaat
     4681 gaaagtcgaa aagcaattca aagaaagtat taataaaggt gataaagtag ttaccattgg
     4741 tggcgtgcat ggaaaaatta ctgaagttgg tgaaactttt tttatgattg agattgacaa
     4801 caacgtaaaa atcaaaattg ataaaactgc cgtttctgca gaagcatcaa agcagtaccg
     4861 caaaacagac gataaaaaat aaaaagcctt acacttgaat attttcaaaa atatcaggtt
     4921 aaaaggcaat gcatcattgc ccgtattttt tatttgtgtt tttgtgtcgc ttgttttctg
     4981 gattttatac tcattgaaca agaattatgt atatcctgta acttatactg ttactcttca
     5041 gaatacacca ttcagcaagc aagtcatcag cgaaatacct caacaggtaa atgtaacact
     5101 tcgttcaaga gggtttgagt tgttttggta tttaatcaga aaccgcgaaa gaaacttaac
     5161 agttgatctg aaaaaatatt ctgtaaacaa tgaaaaactt ggtatcagtt tacaaagtga
     5221 attcagaaat cagaatcaaa accgtttaaa agaaatggat gttgttacaa cactacccga
     5281 ttcagtgtgg ctttatcttt cgtctaagta catgaaaacg gttcccgtag ttacgcagtt
     5341 cacatattcc ataaagaaaa actttgggtt gtcaggtaaa gtcagcattg cgcccgacag
     5401 cattaccatt tccggagaaa aaagtcagct tgacggtgtg aatgaaataa aaactgttgc
     5461 tacagattac gacaatcttg accgtgactt aaaggctacc ctatcattaa tttcacctgc
     5521 cggcaaagtc aatttatcgc agaaagaaat aaagattcac attcctgttg aagaatttat
     5581 cgagaaaaaa attcctgttg ttttaaacta ttctattaac ggcagtggta aggttatact
     5641 cattcctgaa aaagcagaac tcatcattca ggtgccttta aaacgctatg aacaacttag
     5701 tgctgactcg tttcaggtaa cggtttctgc aaaaagatat ttaaaagaag gtcagttggc
     5761 cgttgagtta atccacaagc cacaaggaat aaatgtttca tctatagttc cgtcaacagt
     5821 aaactatttt attgaacgct gatgaaaaaa attggcataa ccggtggcat aggttcggga
     5881 aaaacctttg tagctaatat ttttaaaact ttaggcatac cggtttatga tgcagacaca
     5941 gcagccaaac aattaatgaa ccatgaacca ttgcgcagcc aactcaaaca actttttggc
     6001 aacagtattt ataacgaaca tgatgaactc aacaggcctg ttttggcttc gcttattttt
     6061 aatgatgaaa aaaagttaga cgctgtcaat gccttagtgc atcctgcagt tgcaggacat
     6121 tttagtgaat ggtgcaataa tcaaaaggca gattatattc taaaagaagc tgcaataatg
     6181 tttgagtcag gcgcaaacaa agagttagat gctgtaattt gtgtttctgc acctgaaaca
     6241 gtaagaattg aacgcgccat gaaacgcgat aacacatcag cagaaaaaat aaaagcaatt
     6301 attgccaaac aactgaatga agaagatcgc aacaaacgtt gtaactatct tataaaaaat
     6361 gatggcacag agcctttact gccgcaggtt ctgaaaattc atgatttgtt gaaggcaaaa
     6421 taaaaaggcc gtctgatgca gacagccctt ttacctaacc taaaactaac ctgaaaaact
     6481 ccttattttc tgtgttacgg tatttacaat aacgcaaatc agatgccact attttaattt
     6541 tttatgaatg atatatgaat gttgcataac tttgacatta ttctaaaaat gatgaaagag
     6601 tataaagcat cccgaatgtc ggaagggaat aaagttttgc ccaacaaaat aagaatagac
     6661 aatgatggaa taacattcag aataccgggt gtatttagtg gtgaagaaaa aactattcct
     6721 ttttcaagaa tttcatctgt tgaagttaac acaccgttag tagggttttc ttccatcacc
     6781 attaacacaa cgggcgaagg tgctattgta gcaacaggct tttataaaga agatgtgctt
     6841 gaaataaaaa aaattgtact cgaaaaaatt aatgtttttc agaataagat gtaaaagtta
     6901 tttcgcctct gactctttaa ccttactctg cgc
//
LOCUS       DAOOOI010000098         6880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2162_length_6880_cov_35.012015, whole genome shotgun sequence.
ACCESSION   DAOOOI010000098 DAOOOI010000000
VERSION     DAOOOI010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2162_length_6880_cov_35.012015"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="PKX59_09615"
     CDS             <1..1220
                     /locus_tag="PKX59_09615"
                     /inference="COORDINATES: protein motif:HMM:NF028171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HOZ91163.1"
                     /translation="GFKVNSGEYKVMGLAPYGNRNSAQYKLYKNKISELVDLKTDGSV
                     FLNMKYFNYLTGLNMINENLWSELFEIKPRTAESEINLNHCNLALAIQTITENVILNL
                     AKEAKQITNSDNLCLAGGVALNCVAVGKLNKQNIFKKIYVQPASGDAGGAIGAALAVE
                     HIFLNNKRKVPEKDLMQGCRLGPSYDNHTVTQTLKKHNLHFEEFKEGELLERTARHLA
                     NDKTIAWFQGRMEFGPRALGSRSIFANPATPQIQQTLNQKIKKRESFRPFAPAMLLED
                     YLEFYEGGNENSFMSMTDFLKKEKRLGDDFTSEETGITEQLQKSKSEIQGVTHVDYSS
                     RIQVVSETEDPLFFKLLTEYKKITGRGILINTSFNLRGQPIVCSPDDACKCFLETDID
                     LLVINNFIASKNN"
     gene            complement(1249..2571)
                     /locus_tag="PKX59_09620"
     CDS             complement(1249..2571)
                     /locus_tag="PKX59_09620"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase, eukaryotic"
                     /protein_id="HOZ91164.1"
                     /translation="MDNIKEKFKEKALPMAGTIKDIVKTHGNFVLGQYTVEQVYQGMK
                     GMIGMVTETSKLDANEGIRFRGYSIPELREKLPVSKEGPEPLPEGIFYLLLTGEMPLL
                     KDVIEVNNEWMKRATVPKHVFDMLDMLPDKTHPMTQFSMAIMALQTESVFARKYREGM
                     NKKDYWDPMYEDVMNLIARLPRIAAYIYRSTYKDGKHIEPDPKLDWAGNFAHMLGFEE
                     LDFRKLMRLYLTIHADHEGGNVSAHATHLVGSALSDPYLSLAAGMNGLAGPLHGLAAQ
                     EVARWIIELRDKYNGVPSREQLGEFIKTTLAEGKVVPGYGHAVLRKTDPRYTAQQDFA
                     KKYMADDELFKIVQLLYDVTPEILKATGKIKNPWPNVDAHSGILLMHYGLVEYDFYTV
                     LFGVSRAIGVLTSLLWDRALGMPIERPSSVTTQWIQEKIKSGDAVKAE"
     gene            complement(2603..2872)
                     /locus_tag="PKX59_09625"
     CDS             complement(2603..2872)
                     /locus_tag="PKX59_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91165.1"
                     /translation="MELEESDGEIKKERIRVRKRIRIKKKPDPKKKIKKYLRIFAWIV
                     VIAIFITTLIVLFSESDLRYENTKKPKSRVELNPSTKDFSVNFNL"
     gene            complement(2918..4207)
                     /gene="eno"
                     /locus_tag="PKX59_09630"
     CDS             complement(2918..4207)
                     /gene="eno"
                     /locus_tag="PKX59_09630"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOZ91166.1"
                     /translation="MGNIAEIRARQILDSRGNPTIEVDVYTDNGVVGRAAVPSGASTG
                     AHEAVELRDNDKKIYLGKSVMRAVNNVNAILNKELHGYPVFDQRSIDAAMIHIDGSPN
                     KATIGANAILGVSLACARAAASESGYPLYRYIGGVNARILPVPMMNILNGGSHADNSI
                     DFQEFMIMPVGAESFSESLRMGVEIFHNLKSVLKAKKYSTNVGDEGGFAPDIKSNEEA
                     ILTVIEAIEKAGYKPGDDVMIAMDAAASEFYDAENKVYHFKKSSGEKLTSSEMVSYWK
                     QWCDKYPIVSIEDGLAEDDWAGWKELTNVLGSKVQLVGDDLFVTNTERLARGIKENIA
                     NSILVKVNQIGTLTETINAVEMAKNNSYTSVMSHRSGETEDTTIADLAVALNTGQIKT
                     GSASRSDRIAKYNQLLRIEEMLGGAAEFPGKNFRNLI"
     gene            4316..5065
                     /locus_tag="PKX59_09635"
     CDS             4316..5065
                     /locus_tag="PKX59_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOZ91167.1"
                     /translation="MKVLKIAFGLYAAIVFFITLLCVFPLYFLVFLFFSEKKSPVIAH
                     NLSRIWALILFKSFFIRYKIINGDFISDKGTYVFVANHRSMLDIPLYARACNNTFRFL
                     SKAELTKIPLLGYVIRNLYVTVKRGDKKDRQKSIEAMRKTLESDISIFICPEGTRNFT
                     DQMLLPFKDGAFRLAIATQTPLAVLTVINTDVLLSPESPLALSPGKLVAEWSKPIDTK
                     GMTDDDVESLKQQTAKLMQEILTRHLMSTQK"
     gene            complement(5088..>6880)
                     /locus_tag="PKX59_09640"
     CDS             complement(5088..>6880)
                     /locus_tag="PKX59_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOZ91168.1"
                     /translation="SEVIWDYVDKYRKPTIFAVNQLDLEHANFNATVEKAKQKFGNAI
                     TVMQYPLNQGIGFNAIIDLLKMTMYRFPATGGKPEKLAIPPEEMEYAKKLHNELVEKA
                     AENDEKLMELYFERGSLDEDEMKQGLKAGMMAHQIFPVFCLSAKKDMGSGRLMGFIDN
                     VAPSAIDMPAEIAEDKTLVPCNPTGPTVLFFYKTIIEPHLGKLTFFKVLSGEVSAGTD
                     LMNHKTNTSERLTQLFIMDGKSRNSADKLKAGDLGCTLKLKNTLTNHTLCGKGITINI
                     EPIQFPEPKSRVALIAKNKSDEEKLGEVLSELHLEDPTLVVEYSKELKQVILHAQGDL
                     HLAVTKWRIENVYKMQVEFIKPRISFRETIQKPAVATYRHKKQSGGAGQFGEVHIKIE
                     PYYDGMPDMKEYPLRDKEEHALPWGGKLVFNNCITGGAIDTRFLPSVLKGVMEKMQEG
                     PLTGSFVRDIRVSVFDGKMHPVDSNDISFKIAGMMAFREAFHNAQPQLLEPIYDVEVE
                     MPDDMMGDVMSDLQSRRSIIMGMDSRNGRQVIRAKTPLLELDGYSTALKSITQGKAKL
                     KTSFAEFAPVPGDLQKKLHDEYVKVEALSS"
BASE COUNT         2080 a         1406 c         1126 g         2268 t
ORIGIN      
        1 ttggttttaa agttaattca ggagaatata aagtaatggg cttggcacct tatgggaata
       61 ggaatagtgc acaatataaa ttatacaaga ataaaatcag cgaattagtt gacctgaaga
      121 cagacggttc tgtatttctg aacatgaaat attttaatta cctgacaggg ttaaacatga
      181 taaatgaaaa cctttggtct gagctttttg aaataaaacc acgcactgct gaatcagaaa
      241 taaatttaaa tcattgcaac ttagcattgg caattcaaac gattactgaa aatgtaattt
      301 taaatttagc aaaagaagct aaacaaatta caaattcaga caatttatgt ttggcaggtg
      361 gagtggcatt aaattgtgtt gcagttggca aactcaacaa acaaaatatt ttcaaaaaaa
      421 tttatgttca acctgcctca ggtgatgcag gtggtgccat aggcgctgca cttgcagtag
      481 agcacatatt tttaaacaat aaacgtaaag ttcctgaaaa agatttaatg caaggttgtc
      541 gtttaggtcc ttcttacgac aatcatactg ttacacagac tttgaaaaaa cacaatttac
      601 actttgaaga atttaaagaa ggtgagcttt tagaacgtac tgccagacat cttgcaaatg
      661 ataaaactat agcatggttt caggggcgaa tggaatttgg accgcgtgca cttggatcaa
      721 ggagtatttt tgcaaaccct gcaacaccgc aaattcaaca aacattaaat caaaaaatta
      781 aaaagcgtga atcattcagg ccctttgctc ctgcaatgtt acttgaagat tacttagaat
      841 tttatgaagg aggtaatgaa aatagtttta tgtctatgac tgatttcctt aaaaaagaaa
      901 aaagacttgg tgatgacttt acttcagaag aaacaggaat aactgaacaa ttgcaaaaat
      961 ctaaaagtga aattcaggga gtaacacatg ttgattacag ttcacgaatt caggtggtat
     1021 ctgaaacaga agatcccttg ttttttaaac ttcttacaga atataaaaag ataacaggaa
     1081 gaggaatact cattaatacc agtttcaatc taaggggaca accaattgtt tgcagccctg
     1141 atgatgcctg caaatgcttt ctcgaaacag atattgattt attggtaata aataacttta
     1201 ttgccagtaa aaacaactaa gccaaattcg ctctgaaata aacagcagtt attctgcctt
     1261 tacagcatcg ccagatttaa ttttctcctg aatccattgt gttgttacag aacttggcct
     1321 ttcaataggc atacctaaag cacgatccca taaaagtgaa gtcagaacac caatagctct
     1381 tgatacacca aacaaaacag tataaaaatc atattccacc aaaccataat gcatcagcaa
     1441 gatgcctgaa tgtgcatcaa cgttaggcca tggattttta atcttaccgg tagcttttaa
     1501 aatttccggt gtaacatcat ataataattg aactatttta aacagttcat catctgccat
     1561 atatttcttt gcaaaatcct gctgtgccgt ataacgggga tcggttttac gcagtacggc
     1621 atgtccgtaa ccgggaacaa cttttccttc tgctaatgtt gttttaataa actcacctaa
     1681 ctgctcgcgc gatggaacac cattatactt atcacgtaat tcaataatcc aacgggcaac
     1741 ctcctgtgct gctaaaccat gaagcggacc ggcaagtccg ttcatacctg ctgccaatga
     1801 aagatatgga tcacttaatg cagagccaac aagatgtgtt gcatgtgctg aaacatttcc
     1861 accctcatga tcggcatgaa tggtaagata taatcgcatc aacttcctga aatcaagttc
     1921 ttcaaatcct agcatgtgag caaaatttcc tgcccagtct agtttaggat caggctcaat
     1981 gtgtttgcca tctttataag ttgaacgata gatgtaagca gcaattcgcg gaagacgtgc
     2041 aataagattc attacatctt cgtacatagg atcccaataa tccttcttat tcattccttc
     2101 gcgatactta cgtgcaaaaa cagactccgt ttgcaaagcc ataattgcca ttgaaaattg
     2161 cgtcattgga tgtgtcttat caggcaacat atccaacatg tcaaaaacat gtttaggcac
     2221 ggttgctctt ttcatccatt cattattcac ttcaataaca tccttcagta atggcatttc
     2281 gccagtgagc aagaggtaaa aaattccttc tggcaatggc tcaggacctt ctttagatac
     2341 cggcagtttt tcacgaagtt caggaataga atatcctctg aaacgaattc cttcattagc
     2401 atcaagttta ctggtttcag ttaccatgcc tatcattcct ttcattccct gataaacctg
     2461 ctctactgta tattgaccca aaacaaaatt gccgtgggtt tttacaatgt ctttaattgt
     2521 acctgccatt ggcaaggctt tctctttgaa tttttctttt atgttgtcca tacttgttgt
     2581 ataactgtta cttaaacgtt tgttacaaat taaagtttac agagaagtcc tttgttgaag
     2641 gatttaactc tactcttgat ttaggtttct tggtattttc ataacgaagg tctgattctg
     2701 aaaacaacac tattaatgtt gtaataaaaa tggcaataac tacaatccat gcaaaaatgc
     2761 gcaaatactt tttaatcttc ttcttaggat caggtttctt tttaattcta attcgtttcc
     2821 gaactctgat tctttctttt tttatttcgc catctgactc ttccaattcc attttcaagt
     2881 tgttaattca gattctaaag atacagcccc ttttggatta aatcaaattc ctgaaatttt
     2941 ttccgggaaa ttctgcagct ccacccagca tttcttcaat tcgcaggagt tggttgtact
     3001 tggcaatcct gtcagaacgc gatgccgaac cggtttttat ctgtcctgta tttaatgcca
     3061 ctgccaaatc agcaatagtt gtatcttcag tttcgcctga acggtggctc atcactgatg
     3121 tatagctgtt gttcttcgcc atttcaacag cattgattgt ttctgttaaa gttccaatct
     3181 gattcacttt taccaatatt gagttggcaa tattttcttt tattcctctt gcaagccttt
     3241 cagtgtttgt cacaaacaaa tcgtcaccta ctaactgcac tttactgcct aatacattgg
     3301 ttaattcttt ccatcctgcc caatcgtctt ctgcaagtcc atcttcaata ctaacaatcg
     3361 ggtatttatc gcaccattgc ttccagtagc taaccatttc tgaagatgtc agtttttcac
     3421 cacttgactt tttaaaatga tacactttat tttccgcatc ataaaattca gaagcagcag
     3481 catccattgc aatcataaca tcatcaccgg gtttgtagcc tgccttttct attgcttcta
     3541 tcactgttaa tattgcttct tcattagatt taatgtcagg tgcaaaaccg ccttcatcac
     3601 caacgttggt agaatatttc ttagctttta aaacactctt cagattgtga aatatttcaa
     3661 cacccattcg tagtgattca gaaaaacttt ctgctccaac aggcataatc ataaattcct
     3721 gaaagtctat actgttatca gcatgagatc ctccatttaa gatgttcatc attggcacag
     3781 gcaatatacg tgcattaaca ccaccgatat aacgatatag cggatagccg gactcacttg
     3841 cagctgctct tgcacatgct aatgaaacac ccaaaatagc attggcacct attgtagctt
     3901 tattaggtga accatctata tgaatcattg ccgcatcaat actgcgctga tcaaaaacag
     3961 gataaccatg cagttcttta ttcaaaatgg catttacatt atttacagct ctcatcacac
     4021 ttttgccaag ataaatcttc ttatcattat cacgcaactc aacggcttcg tgtgctccgg
     4081 ttgatgcacc tgaaggtaca gcagccctgc ccactacacc attgtctgta tatacatcta
     4141 cttcaatagt tggatttcct cttgagtcga gtatttgtct tgctctgatt tcagcaatat
     4201 tgcccataat attttacact ttataaaacg gctgcaaagt taacgtaatg tgccgtaaat
     4261 ttatttaatt tagcgaaata aatttcaacc acatctataa cactaatagc gctatatgaa
     4321 agtgcttaaa attgcatttg gattatatgc agcaattgta ttttttatca ctttgctttg
     4381 tgttttccct ctatattttt tagtattttt atttttcagt gaaaagaaat ctcctgttat
     4441 agcacataac ctttctcgta tttgggcatt gatattgttc aaaagttttt ttattcgcta
     4501 caagataatc aatggtgatt ttatttcgga taaaggaacc tatgtatttg ttgccaacca
     4561 tcgctctatg ctggatattc cactctatgc gcgtgcatgt aacaatacct tcagattttt
     4621 atctaaagcc gaactgacta aaatcccgtt actgggttat gtaatccgaa atctgtatgt
     4681 cactgtaaaa cgaggtgata aaaaagacag acaaaaaagt attgaggcaa tgcgtaaaac
     4741 acttgagtcg gatatttcaa tatttatttg tcctgaaggc actcgtaatt ttacagatca
     4801 gatgttgctg ccatttaaag acggtgcatt tcgtctggct attgcaacac aaacaccttt
     4861 ggcagtactc acagtaataa atacagatgt attgttgtca cctgaatcac cattagcttt
     4921 gagtccgggt aaactggtag cagaatggag taaacctatt gacaccaaag gaatgactga
     4981 tgatgatgtt gaatcactaa aacaacagac agcaaaactg atgcaagaaa tattgacacg
     5041 tcatttaatg tcaacacaaa aataattttt gtttttgttg attaatttta actgcttagt
     5101 gcttccactt tcacatattc atcgtgtaat ttcttttgta agtctccggg cacaggagca
     5161 aattcagcaa atgatgtttt taacttagcc tttccttgtg tgatggattt taatgcagtg
     5221 ctgtaaccat caagttcaag caaaggagtt tttgcacgaa ttacctgtct tccatttcga
     5281 gagtccatac ccataatgat actccttctc gactgcaggt cactcattac atcgcccatc
     5341 atatcatcag gcatttctac ttcaacatca taaataggtt ctaaaagttg tggctgtgca
     5401 ttgtgaaatg cttcccgaaa tgccatcatg cctgcaatct taaatgatat atcatttgaa
     5461 tcaacaggat gcattttacc atcaaacaca cttactctta tatcacgcac aaacgatcct
     5521 gttaacggac cttcctgcat tttttccatt acgcctttaa gaactgaagg taaaaagcgt
     5581 gtatcaatag cacctcctgt aatacaatta ttaaaaacta attttccacc ccagggtaat
     5641 gcatgctctt ctttatcacg aagtggatat tctttcatat caggcatacc atcatagtaa
     5701 ggttcaattt ttatgtgcac ttcaccaaat tgacctgctc cacctgattg ttttttatgt
     5761 ctgtaagttg caactgcagg tttttgaatt gtttcgcgga acgatatgcg tggttttata
     5821 aactcaacct gcattttata tacattttca atccgccatt ttgttactgc aagatgtaaa
     5881 tcgccttgcg catgtaaaat aacctgcttg agttctttag aatattctac tactaatgta
     5941 ggatcttcaa gatgtaattc actcagcacc tcgcccaatt tttcttcatc gcttttgttt
     6001 tttgcaatga gtgctactct ggattttggt tcaggaaatt gtattggttc aatattaata
     6061 gttatacctt ttccgcaaag cgtatggttt gtaagtgtat tcttcaattt aagagtacat
     6121 cctaaatcgc ctgccttaag tttatcagct gaatttctac tctttccatc catgataaac
     6181 aactgggtca atctttcgct tgtatttgtt ttgtgattca ttaaatccgt ccctgcactt
     6241 acctctccag aaagaacttt aaaaaaggtc aacttaccca aatgtggctc aattatagtt
     6301 ttataaaaaa acaaaactgt tggtcctgtt ggattacatg gaactaaggt tttatcttca
     6361 gcaatttctg ccggcatgtc aattgccgaa ggtgcaacat tatcaataaa ccccatcagt
     6421 cgtccgctgc ccatatcttt ctttgccgat aagcagaaaa caggaaaaat ctgatgtgcc
     6481 atcattcctg ctttcaatcc ttgtttcatt tcatcttcgt caagagatcc tcgctcaaag
     6541 tataactcca tcagtttttc atcattctca gcagctttct ccaccaattc attatgcagc
     6601 ttttttgcat actccatttc ttctggtggg atggcaagct tctcaggttt gccgcctgtg
     6661 gcaggaaaac gatacatggt cattttcaat aaatctatta tcgcattaaa tcctatccct
     6721 tgatttaacg gatattgcat aacggtaatg gcattgccga acttctgttt tgctttttct
     6781 acagttgcat taaaattggc atgttcaagg tcaagctgat tgactgcaaa tatggttggc
     6841 ttccggtatt tatccacgta atcccaaata acctcactgc
//
LOCUS       DAOOOI010000099         6786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2201_length_6786_cov_9.959739, whole genome shotgun sequence.
ACCESSION   DAOOOI010000099 DAOOOI010000000
VERSION     DAOOOI010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2201_length_6786_cov_9.959739"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PKX59_09645"
     CDS             <1..653
                     /locus_tag="PKX59_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91169.1"
                     /translation="PAMIVGPVQLLANNTIGYLQQIEFLALMAITGATTSSKPLMCNA
                     ANLAFEKEAYMQCEQNLNRTASGDDTFLMLAIKYKLHTNIVFIKNKHAIVKTNACNNW
                     QTLKQQRIRWAAKTKYYTHRYIYLFGLGILASNLFMAIAISFCMVSPIYCSLIILIKL
                     LADGIIVQSSNQFFKQQIKLHHFLMVYAIYPFYSIYIYYRARKNKFVWRNRNWINQ"
     gene            756..1340
                     /locus_tag="PKX59_09650"
     CDS             756..1340
                     /locus_tag="PKX59_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOZ91170.1"
                     /translation="MKKNFLIALLALAFTNVNAQNKWNLDASHSNVNFTVSHLVVSEV
                     DGNFKIFNGDIVAKDDSFEDATINFNVDVASINTENKDRDAHLKSDDFFNAEKFPKMS
                     FKSSAFKKVNGNKYVLKGFLTIRDVTKPVSFDVTFGGVVKDPWGNTKAGFKATTTISR
                     SAYKLLWNKTIETGGAVVGDEVTITVKTEFAKAK"
     gene            1425..1916
                     /gene="folK"
                     /locus_tag="PKX59_09655"
     CDS             1425..1916
                     /gene="folK"
                     /locus_tag="PKX59_09655"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013667271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOZ91171.1"
                     /translation="MAVAYLLLGGNQGETQNTFIRTIELIKLRIGAIKTQSSIYKTAA
                     WGNTNQPDFLNQVIEVNTLLHPNHLLNQILSIELELGRKRDILWGQRIIDIDILFYDA
                     LIYDSIELTIPHKYLHTRMFALIPLHEIAPQLVHPILQKNIKALIEACDDKLAVTKIS
                     KTD"
     gene            complement(2035..2517)
                     /gene="rplQ"
                     /locus_tag="PKX59_09660"
     CDS             complement(2035..2517)
                     /gene="rplQ"
                     /locus_tag="PKX59_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOZ91172.1"
                     /translation="MRHGKKINHLGRTASHRDAMLSNMAASLIIHKRINTTLAKAKAL
                     RKVVEPLITKSKTDSTHSRRTVFSLLQSKEAVQELFKNVSAKVGDRPGGYTRIIKLEN
                     RLGDNAEMCMMELVDYNEIYTGNTKAVVEKTKTTRRRGGAKKKATKSEATEAADSTEA
                     "
     gene            complement(2582..3574)
                     /locus_tag="PKX59_09665"
     CDS             complement(2582..3574)
                     /locus_tag="PKX59_09665"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOZ91173.1"
                     /translation="MAILTFQKPDKVVMNHSSETVGEFEFRPLEPGYGVTIGNALRRI
                     LLSSLEGYAITSIKVAGVDHEFSTIKGVVEDVIEMVLNLKQVRFKRQIEGTTFEKVTV
                     VVSGKEQFTAGDIAKHTTAFQVLNPELVICNMEQSVKLQLDITIDKGRGYVPAEENKN
                     ANQLQGTIPIDSIYTPIKNVKYYVENYRVEQKTDYEKLIMEITSDGSIHPKDALKDAA
                     EILIHHFMLFSDEKITLESIKKTPTEEFDETSLHMRQLLKTKLVDMDLSVRALNCLKA
                     ADVETLGDLVSYNKSDLLKFRNFGKKSLTELEDLVRSKNLTFGMNLAKYKLDKD"
     gene            complement(3636..4241)
                     /gene="rpsD"
                     /locus_tag="PKX59_09670"
     CDS             complement(3636..4241)
                     /gene="rpsD"
                     /locus_tag="PKX59_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOZ91174.1"
                     /translation="MARYTGPKTKIARKFREAIFGPDKYFDKRSYPPGQHGVNKKRAK
                     QSEYAVQLSEKQKVKYTYGILERQFAKTFDRASRMQGITGENLLKLIESRLDNVVYRM
                     GIAPSRAAARQLVGHCHIQVNETVVNIPSFTVRAGDIISVRERSKSLEVITDSLKSRS
                     NKFSWIEWDGALMTGKFIATPERSEIPENIKEQLIVELYSK"
     gene            complement(4297..4698)
                     /gene="rpsK"
                     /locus_tag="PKX59_09675"
     CDS             complement(4297..4698)
                     /gene="rpsK"
                     /locus_tag="PKX59_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOZ91175.1"
                     /translation="MATQKTAASKTVKKKVVKVEATGVAHVSATFNNIIISLTNLSGQ
                     VISWSSAGKMGFKGSKKNTPYAAQMAASDCAKVAYDLGLRKVKVIVKGPGAGRESAIR
                     TINAAGIEVSEILDVTPIPHNGCRPPGRRRI"
     gene            complement(4773..5150)
                     /gene="rpsM"
                     /locus_tag="PKX59_09680"
     CDS             complement(4773..5150)
                     /gene="rpsM"
                     /locus_tag="PKX59_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOZ91176.1"
                     /translation="MARISGIDLPKHKRGEIGLTYIYGIGRSTAQYILGKAGIPVDKK
                     VDEWNDKELTDIRTIINDEIKVEGSLRSEVQLNIKRLMDVGSYRGIRHRSGLPVRGQR
                     TKNNCRTRKGKRKTVANKKKVTK"
     gene            complement(5196..5312)
                     /gene="ykgO"
                     /locus_tag="PKX59_09685"
     CDS             complement(5196..5312)
                     /gene="ykgO"
                     /locus_tag="PKX59_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOZ91177.1"
                     /translation="MKVKPSLKKRSVDCKIVRRKGKLYVINKKNPRFKQRQG"
     gene            complement(5353..5571)
                     /gene="infA"
                     /locus_tag="PKX59_09690"
     CDS             complement(5353..5571)
                     /gene="infA"
                     /locus_tag="PKX59_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOZ91178.1"
                     /translation="MSKVSAIEQDGIIREALSNAMFKVELENGHEVIAHISGKMRMNY
                     IKILPGDKVKMEMSPYDLTKARIVYRYK"
     gene            complement(5564..6367)
                     /gene="map"
                     /locus_tag="PKX59_09695"
     CDS             complement(5564..6367)
                     /gene="map"
                     /locus_tag="PKX59_09695"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOZ91179.1"
                     /translation="MDIKYKTAEEIESIRVSSLLVSETLAHVAQFIKPGVTTLELDKS
                     AEDFIKSKNAIAAFKGFKGFPGTLCISVNAEVVHGIPTKRVVRDGDVLSVDCGVIQNG
                     WYGDSAYTFGVGQIDQSDIQLLQVTQQCLMQGIAKVKVGNRMGDVSSAIQEYAELHKM
                     GVVRELIGHGIGRNLHEKPDVPNYGSRGQGINLKAGLVIAIEPMINKGKRGIKFHDDG
                     WTVTTIDMKNSAHFEHTVAVTQNGYDVLSSFEPIQSAIEKNINIYNINV"
     gene            complement(6397..>6786)
                     /locus_tag="PKX59_09700"
     CDS             complement(6397..>6786)
                     /locus_tag="PKX59_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HOZ91180.1"
                     /translation="IIIFTYFYTAIAVNPNQMADDMKKNGGFIPGVKPGRKTADFIDD
                     VMSRITLPGSLFLAFVAIMPALASIGGVNQNFAQFFGGTSLLILVGVVLDTLQQIESQ
                     LLMRKYDGLMKNGRIKGRSAVGSGVAY"
BASE COUNT         2011 a         1325 c         1105 g         2345 t
ORIGIN      
        1 agcctgcaat gatagttggg ccggttcaac ttttagctaa caacactatt ggatatttac
       61 aacaaataga atttttggcg ttaatggcta taacaggcgc aaccacttcg agcaaaccat
      121 taatgtgtaa tgcagccaac ttagcttttg aaaaagaagc atatatgcag tgcgaacaaa
      181 atttaaaccg aactgcaagt ggtgatgata cctttttaat gctagccata aaatacaagc
      241 tacatactaa tatagtcttt ataaaaaaca aacatgcaat agtaaaaacc aatgcctgta
      301 ataactggca aaccttaaaa caacaacgaa tcagatgggc cgctaaaact aagtactaca
      361 cgcaccggta catatactta tttggcttag gtatattggc aagcaattta tttatggcaa
      421 ttgctatttc tttttgcatg gtgtcgccaa tttattgtag tttaattatc cttatcaagc
      481 ttctggctga tggcataatt gttcaatcaa gtaatcaatt ttttaaacaa caaattaagc
      541 tgcatcactt tttaatggta tatgccattt accccttcta ttcaatttac atatattatc
      601 gtgcgcgaaa gaataaattc gtttggcgca ataggaactg gataaaccaa taaatgagtt
      661 ttaaagtagc aaagtaataa aatggctttt gattttgaat attatatgta catacatata
      721 tttgtacccg atttaactta aactcaataa ttaaaatgaa aaagaacttc ttaatagctt
      781 tgctcgctct ggcttttacc aatgtaaatg cacaaaacaa atggaattta gatgccagcc
      841 atagcaacgt aaattttacc gtatctcatt tagtagttag tgaggtagat ggcaatttta
      901 aaatttttaa tggcgatatt gttgctaaag atgattcatt tgaagacgcc acaattaatt
      961 tcaatgttga tgtagccagc attaacactg aaaataaaga tagggatgca caccttaaaa
     1021 gtgacgactt ttttaatgcc gaaaaatttc caaaaatgtc ttttaagagt agcgcattta
     1081 aaaaagtaaa cggcaataaa tatgttttaa aaggattttt aactatcaga gatgttacta
     1141 agcctgtatc gtttgatgtt acctttggtg gtgtggttaa ggacccgtgg ggcaatacca
     1201 aagcaggttt taaggctaca acaacaatta gccgttcggc ctataaactg ttatggaata
     1261 aaaccataga aaccggtggt gccgttgttg gcgatgaagt aacgattacc gtaaaaactg
     1321 aatttgctaa agcaaaataa ttaaaaccat taagcgaaaa aaaacggctt gcaattatca
     1381 gcaagtcgtt tttttttgtt tcgttgcatt gaattaaaat atttatggct gtagcttatt
     1441 tactgttagg tggtaaccaa ggcgaaacac aaaacacttt tatcaggaca attgaattaa
     1501 taaaacttcg aattggtgct ataaaaacac aatcatccat ttacaaaact gcagcttggg
     1561 gcaataccaa tcagcccgat tttttaaatc aggttataga agtaaatacc ttactccacc
     1621 ccaaccattt actaaatcaa atactaagta tagagcttga gctaggccga aagcgcgaca
     1681 tactatgggg tcaacgtata attgacattg acatactttt ttatgatgct ttgatttacg
     1741 atagcattga acttaccatt ccacacaagt atttacatac aagaatgttt gcacttattc
     1801 cacttcatga aattgcacca caattggtac atccgattct acaaaaaaat ataaaagcac
     1861 tcatcgaagc gtgtgacgat aagttagcgg ttacaaaaat ttcgaaaact gattagaatc
     1921 gtaattatcc atcggataat caattattac tttacagcag tgtttaattt taaagaacaa
     1981 aaaagccgct aatcttttca gattgcggct tttattttaa aatttcaaac taaactatgc
     2041 ttctgttgaa tcagcagctt cagtagcttc agatttagtg gctttctttt tagcaccacc
     2101 acgtctgcgg gttgtttttg ttttctcaac aacagctttt gtgttgcctg tataaatttc
     2161 attataatca accaactcca tcatacacat ttctgcatta tcgcccaatc tgttttccag
     2221 cttaattatg cgtgtatatc cacccggacg gtcgccaact ttagctgata catttttgaa
     2281 taattcttgt acagcctctt tgctttgcaa gagcgagaaa acggttctac gactatgtgt
     2341 tgaatcggtt ttcgattttg taatcaaagg ttcaacaact ttacgtaatg ctttcgcttt
     2401 agccaaagtg gtattaattc ttttgtgaat tataagcgat gcagccatat tgcttaacat
     2461 cgcatcacga tgactggctg ttcgtcctaa atgatttatt ttttttccgt gtctcattac
     2521 ttttctgttt taattgtagg ctgttcaggc atcaaaagac accagagttg ctcaaatctt
     2581 attaatcttt atctaatttg tatttggcta aattcattcc aaaagtaagg ttcttacttc
     2641 taaccaagtc ttctaattca gtaagtgatt tcttaccaaa gtttctgaat ttcaacaagt
     2701 ctgacttgtt gtacgaaacc aaatcgccta atgtttcaac gtctgctgct tttaaacagt
     2761 ttaatgcacg taccgacaaa tccatatcaa ccaattttgt ttttaataat tggcgcatgt
     2821 gtaatgatgt ttcatcaaac tcttcagttg gcgttttctt gatagactcc agcgtgattt
     2881 tttcatcact aaataacata aagtgatgaa tcaatatttc agcagcatcc tttaatgcat
     2941 ctttcggatg aatagaacca tcggaagtaa tttccatgat caatttttca taatccgttt
     3001 tctgttctac acgataattt tctacgtaat actttacatt tttaattggc gtgtaaatag
     3061 aatcaattgg aatagtgcct tgtaactgat tggcattttt gttttcctca gccggaacat
     3121 aaccacgtcc tttgtcaatg gtaatatcaa gctgaagctt tactgattgc tccatattac
     3181 agattaccaa ttcaggattc aacacctgaa atgcagttgt atgtttggca atatcaccgg
     3241 ccgtaaattg ctctttacca cttactacta cggtaacttt ttcgaatgtt gtgccttcaa
     3301 tttgtcgttt aaatcgtacc tgtttaaggt ttaacaccat ttcaattaca tcttcaacaa
     3361 cacctttaat ggtagaaaac tcgtgatcaa caccggcaac tttaatactg gtaattgcat
     3421 aaccttctaa tgaagaaagt aatatacgcc ttaatgcatt tccaatggta actccgtagc
     3481 caggttcaag tggacgaaac tcaaactcac caacagtttc tgatgagtga ttcattacaa
     3541 ctttgtctgg tttttggaat gttaatatgg ccatttgttt ttaatttaat tttttgtttt
     3601 agaattattt cttttcaact aatccttggc tattattact tagagtacaa ttcgacaata
     3661 agttgttctt taatgttttc aggtatttct gatcgctcag gtgtagcaat aaatttaccg
     3721 gtcataaggg caccatccca ctctatccat gaaaatttgt tgctgcggct ttttaagcta
     3781 tctgtaatta cttcgagcga tttagaacgt tcacggacag aaattatgtc acccgctctt
     3841 actgtaaatg aagggatatt tactaccgtt tcatttacct gaatgtggca atggcctact
     3901 aactgacgtg cagcagcacg cgaaggtgca atacccatac ggtaaactac attgtctaat
     3961 ctcgattcga taagctttaa taggttttcg cctgtaatac cttgcatacg tgatgcacga
     4021 tcgaatgttt tagcgaactg gcgctctaaa atgccataag tatattttac tttttgtttt
     4081 tcagaaagct gcacagcgta ttctgattgc tttgcgcgct ttttgtttac accgtgttgt
     4141 cccggagggt aacttctttt gtcaaaatat ttatccggac cgaagatagc ctcacgaaat
     4201 ttacgagcaa ttttagtttt agggcctgta taacgtgcca ttataattgt tgtttttaat
     4261 ttttaattga tttttaaata gcagaattcg tattaattaa attcttctac gacccggagg
     4321 gcggcagcca ttgtgcggaa ttggtgttac gtctaatatt tccgaaactt cgatacctgc
     4381 tgcattaata gtacgtattg ctgattcgcg acctgcaccg ggtcctttta cgataacctt
     4441 tactttacgc aaacctaaat cataggctac cttagcacaa tcgcttgctg ccatttgagc
     4501 tgcatatggt gtgtttttct ttgaaccttt aaagcccatc ttacctgcac tcgaccatga
     4561 tattacctga cctgaaaggt tggttaaaga aataattata ttattaaaag ttgcactaac
     4621 atgggctacg cctgttgcct caacctttac aacctttttc tttaccgttt tacttgctgc
     4681 tgttttttgt gttgccattg ctttttatta atatgcttaa atccttggcg ggatgattaa
     4741 ttgtaaattg atattactgt tgcatttaaa gattacttgg taaccttttt cttattagca
     4801 actgttttgc gtttaccttt gcgggtacgg caattgtttt ttgtacgctg gccgcgtact
     4861 ggtaaaccac tgcgatgtct gataccacga tacgaaccaa catccattaa acgcttaatg
     4921 tttaactgaa cttcggagcg aagcgaccct tctaccttta tctcatcatt aatgatggtt
     4981 cggatatcgg tcaattcttt atcattccat tcatctactt ttttatctac cggaatgcca
     5041 gccttgccca atatgtattg cgcagttgaa cggccgatgc catatatata ggttaaacct
     5101 atttcgcctc ttttatgttt tggtaaatct atacctgaaa tacgagccat gtattcgttt
     5161 ttttaatttt taaatttttt aaattgaaca atcgtttaac cttgacgttg cttaaaacga
     5221 gggttctttt tgttgattac ataaagtttg cctttacgtc ttacaatttt gcaatcaaca
     5281 ctgcgctttt ttaatgatgg ttttactttc atttttattc aaattggtgg cggcttttat
     5341 gctaccgggt aattatttat atctgtaaac tatgcgagcc ttagttaaat catagggcga
     5401 catttccatt ttcactttat ctccaggtaa tattttaatg tagttcatac gcattttacc
     5461 tgaaatatgc gcaattactt catgtccatt ttcaagctca accttaaaca ttgcattgct
     5521 taatgcttcg cggattattc catcttgctc tatggcacta actttagaca tttatgttgt
     5581 atatatttat atttttttca attgcacttt gtataggttc gaatgatgat agtacgtcat
     5641 atccattttg agttactgct actgtatgtt caaagtgtgc cgagtttttc atatcaatag
     5701 tagttactgt ccatccatca tcatgaaact ttatcccacg tttcccttta tttatcattg
     5761 gttcgatagc aattacaagc cctgctttta aatttatccc ctgccctctg ctgccatagt
     5821 ttggcacatc tggcttttca tgcaaatttc ggccgatacc atgcccaatt aattcccgaa
     5881 caactcccat tttatgtaat tcagcgtact cctgtatggc actggaaaca tcacccattc
     5941 tattaccaac tttaactttt gcaatacctt gcataaggca ttgttgtgtt acctgcaata
     6001 attggatatc cgactggtca atttgcccca ctccaaaagt gtaagctgaa tcgccatacc
     6061 aaccattttg aattacgcca caatcaaccg ataatacatc gccatcacga actactcttt
     6121 tggttggaat gccatgcacc acttctgcat taactgaaat acataaggtg cccggaaatc
     6181 ctttaaagcc tttaaaggca gcaattgcat ttttgctttt gataaaatct tcagctgatt
     6241 tatccaattc aagggttgta acaccaggtt taataaactg tgctacatgt gctaaagttt
     6301 cactaacaag taaagaactc actcttattg attcaatctc ctctgccgtc ttatatttta
     6361 tatccactgt attatactat tcttggtttg cgatttttaa tacgcaacgc cacttcctac
     6421 tgccgacctg cctttaattc ttccattttt cattaaacca tcgtatttac gcattaacaa
     6481 ttggctttct atttgttgca aagtatccaa aaccacaccc actaaaatca aaagggatgt
     6541 gcctccgaaa aattgtgcaa agttttgatt tacgccacca atgctggcca atgctggcat
     6601 aatggctaca aatgctaaaa acaatgagcc tggcaaagta atacgcgaca ttacgtcatc
     6661 aataaaatct gccgttttac gtcctggttt cacacctgga ataaagccac catttttctt
     6721 catatcatca gccatttgat ttggatttac ggcaatggct gtatagaaat aggtaaatat
     6781 gataat
//
LOCUS       DAOOOI010000100         6656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2245_length_6656_cov_8.924860, whole genome shotgun sequence.
ACCESSION   DAOOOI010000100 DAOOOI010000000
VERSION     DAOOOI010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6656
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2245_length_6656_cov_8.924860"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..4584
                     /locus_tag="PKX59_09705"
     CDS             31..4584
                     /locus_tag="PKX59_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91181.1"
                     /translation="MKRLFTTLSLGLATMVGFAQSNCYFSEDMETIDSVTSTPANAWV
                     QNTRLSVSGVACDSAQVLNGTDAILETDPIDLTGFFFVSVTFQHICRIDFFDAANVDV
                     SIDGGVTWTTLGPAEFDSTLVDYPAFFGQAYFSSATTLDWDPGVPGTMASNSMWHLAR
                     FNLSQWGGVSDFRMRFRLSDVNGGGAGGYPGWFIDDICIQAAVCELNPPTIAFNSGYP
                     QGTIYNLGPYNVQSHITDASGIMSATLTYTINNNPPVSVLMTDLGNGDYLGQIPAVND
                     GDSICYSITAVDNSGCANSAVFPTSGCITFYASQGITFPFCDNFDLQNLWTDSLGTAG
                     STPWQRGQSTVWPGSAHSAPNIWEVGLDQQYVGSCLSYLYSPTFSFLGVFNAQIELWI
                     NSNCENFWDGVRLEYTTDNGATWNVLGTNNTTFPTGQNWYNDGQLNSSQTYAWTGTSQ
                     VIAGTPGWFKAMHTLSMLDNQTSVMFRFVFTSDASVQADGFAIDDVCIFVPPPQDAGV
                     QLISQPPVQAPAGLCIPVEVTITNFGSQPITSTPVTYVASTGATQTATWSGNLAPGAS
                     TVFTITPCFTIPSGPFSMCAYTSLPNDGLPFNDTTCISSTGIPVLTLTSCDDFENGNV
                     GWLSTPTGGSNEWQLGTPAFGATTGAYSGVNAWDINLNTGYVQNEIDTLYTPIYDITG
                     LSASNLTMSFFSNYSTANTDGFWVEYTIDGTNWSVLGTMGDPNASNWYTTANLDFTNN
                     PAFNNNSSGWKKSTYFLNNLLITSPTTIRFRFIFYATTFGTGGVGVSLDNFCVSIPCS
                     PDAGVTAVTSYNSSIGNTLMQGSQDSVQVTIHNYGTAALTSLNITYAVNGTPVLPPYA
                     WTGNLAPNGNATIILPTPYTTPGGQYTITAWTDLSGDCNSANDTLAGAAVGVPVIVVD
                     FNNSYCDDFENGNIGWSTKNNSTTPGSDWQFGTPTFGTTNSAYSGVNCWDINLNSVYG
                     ANANSELYTPIFDLTNAVDATISFWQNVASESNWDGTRMEYRIGNGAWTILGTPCDTL
                     TMGQFNWYNENQLNSSQQYAWTQGLATCPLPTGWIRSMRKLDPIFNNQPMVQFRYIFT
                     SDGSVQTNGYSIDDFCVTVPVPLTVTPVAVSTANPGPPFIFPGQSMPFKSKIYNDGTS
                     AVNNFVTTLSVDGNIISNDTVTLTTPLNPNTNINYTYVNGWIASPGFHNVCVSTYFPN
                     QSADLKPIGDTICYSVQVFDSATVGAGASSKCYDFESGYRWVTLNSTSYSANSSWDLG
                     TSVKLGGNHSPVNSWFTSLAGQYPNRDTSALFSPVFTLTGGLTYNINFWHSFMTERNE
                     DGGTFEYSTNLGATWTQLGDDHDPNWMNTYSITALGPVPPVHPGWSGNSGGWGQVGHD
                     ICLPTAGPQQVIFRWRFNSDFSVRDEGWAIDDVCLEVKTPITLCTTGLTDLNNTFDMI
                     QNTPNPFSDYSVITFNLSDNGSTQLDIVDVTGKVLMTPVNANLSAGTYNITVSAENLA
                     SGVYFYVLKHNDRQLVKKMVIAK"
     gene            4709..4782
                     /locus_tag="PKX59_09710"
     tRNA            4709..4782
                     /locus_tag="PKX59_09710"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4743..4745,aa:Ala,seq:ggc)
     gene            4898..5770
                     /gene="lipA"
                     /locus_tag="PKX59_09715"
     CDS             4898..5770
                     /gene="lipA"
                     /locus_tag="PKX59_09715"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOZ91182.1"
                     /translation="MEEKAKERLYKKPEWLKVKLPIGEEYRKVRGIVDNNKLHTICES
                     GNCPNMGECWGAGTATFMILGNICTRSCKFCAVATGKPLAVDKTEPQRVAESVKLMKV
                     KHCVITSVDRDDLKDGGSEIWAETIRKVREISEGTTMETLVPDFAGKWENLERVMQER
                     PEIMSHNLETVRRLTKEVRVQAKYDRSLEALKRINAFGLRTKSGVMLGLGETRDEVLE
                     TMNDLYQNGVHILTLGQYLQPTKEHLPIAEFVNPAVFKEYKQIGLEMGFKYVESGPLV
                     RSSYHAERHLTFDV"
     gene            5783..>6656
                     /locus_tag="PKX59_09720"
     CDS             5783..>6656
                     /locus_tag="PKX59_09720"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOZ91183.1"
                     /translation="MTRVAINGFGRIGRTVLKKLLLNKNLTIVGINDLTDPKTLAHLF
                     KYDSIHGKFDGTVTSAEKAIIINNQTIPVYAEKDPAMLPWKDLQVDVVIESTGRFTKA
                     ELAEAHIKAGARKVIISAPGSGNLKSVVLGVNENILDGSEKIISNASCTTNNAAPMIK
                     ILQDNWGVESAYITTVHAYTGDQNIHDAPHKDLRRARAAAQSIIPTTTGAAKAVTEIF
                     PDLKGMIGGAGIRVPVIDGSITDITCILKHDATVEAINQKFLEASQGSMKGIIEYTTD
                     PIVSVDIIGNTHSCI"
BASE COUNT         2053 a         1283 c         1326 g         1994 t
ORIGIN      
        1 atcacaaata ttccttaaaa tcaaaaagta atgaaaagac tgtttactac actaagtctt
       61 ggattagcaa cgatggtagg ctttgcccaa tcgaattgtt atttctcaga agacatggaa
      121 acaattgact cagttacatc aactccggcc aatgcctggg ttcaaaacac acgccttagc
      181 gtgtcgggtg tagcctgcga ctcagcacaa gtgctcaatg gcactgatgc tattcttgaa
      241 accgatccaa tagatttgac aggtttcttc tttgtaagcg taaccttcca gcatatttgc
      301 cggattgact tttttgatgc agccaacgtg gatgtgtcaa ttgacggagg tgtgacatgg
      361 acaactttag gtccagcaga atttgattca acattagttg attaccctgc gttttttggc
      421 caagcgtact tttcttctgc aacaactttg gactgggatc cgggagtacc cggaacaatg
      481 gcctcaaaca gtatgtggca cttagcacgc tttaacctgt cacaatgggg tggcgtatct
      541 gacttcagaa tgcgtttccg tctatcagac gtgaatggtg gcggtgcagg cggttatccc
      601 ggttggttta ttgatgacat ctgtattcag gctgctgttt gcgagctaaa ccctccaaca
      661 atagcgttta acagtggtta tccacaggga accatttata accttggacc ttataatgta
      721 cagtcacata ttactgatgc ttcaggcata atgagtgcta ctttaactta tacaataaat
      781 aataaccctc ctgtaagtgt actcatgaca gaccttggta atggcgacta cttaggacaa
      841 attcctgctg taaatgatgg tgattccatt tgctactcaa tcacagcagt ggataactca
      901 ggctgtgcca actctgcagt tttccctaca tcaggatgta taaccttcta tgcatcgcaa
      961 ggaattacct tcccgttttg tgacaacttt gatttgcaaa atttatggac ggactcactt
     1021 ggcactgcag gttcaacacc atggcaaaga gggcaatcaa ccgtttggcc tggctctgca
     1081 cattctgcac ccaatatatg ggaggttggt ttagatcaac aatatgttgg aagttgtctt
     1141 tcttatttat attcacctac attctcattc ttaggggtat ttaatgcaca gattgagtta
     1201 tggataaatt caaattgtga aaatttctgg gacggtgttc ggttagaata tactacagat
     1261 aatggcgcta cctggaatgt acttgggact aataacacaa ctttccctac agggcaaaac
     1321 tggtataatg atggtcaatt aaactcgagt cagacgtatg catggacagg cacaagtcag
     1381 gtcatagcag gtacaccggg ctggtttaaa gccatgcata ctttgagtat gttagataac
     1441 caaaccagtg tgatgttccg ttttgtattt acctctgatg catcagtaca agcagatggt
     1501 tttgcaattg atgacgtttg tatttttgtt ccaccaccac aagatgcagg tgtacagtta
     1561 atatctcaac ctcctgtaca ggctcctgcc ggtctttgta ttcctgtaga agtaactatt
     1621 accaactttg gtagtcaacc aataacttct actccggtta cttatgttgc aagtacaggt
     1681 gcaactcaaa cagcaacctg gtcaggtaac cttgctcccg gagcatcaac agtatttact
     1741 attacacctt gctttaccat tccttcagga ccattttcaa tgtgtgccta tacttcatta
     1801 ccaaatgatg gattaccatt taatgatact acttgtatca gtagcacggg tattcctgta
     1861 cttacattaa catcctgtga tgattttgaa aatggtaacg ttggttggct atcaacacca
     1921 acaggtggtt ctaatgaatg gcaattaggt acaccggcat ttggtgctac cactggtgct
     1981 tattcaggag taaatgcatg ggacattaac cttaatacag gatatgtaca aaatgaaatt
     2041 gataccttat atacaccaat ttatgatata acaggtttat cagcaagtaa ccttacaatg
     2101 tcattcttca gtaattacag tacagcaaat actgatggtt tctgggttga atacacaatt
     2161 gatggtacaa actggagtgt attaggtaca atgggcgatc caaatgcttc aaactggtac
     2221 accactgcca atcttgattt taccaataat cctgcattca ataataattc ttcaggctgg
     2281 aaaaaatcta cttacttcct gaataacctg ctaattactt caccaactac aattcgtttc
     2341 agatttatat tctatgcaac gacatttggt acgggtggtg ttggtgtgtc tcttgacaac
     2401 ttctgtgtta gcataccttg ttcacctgat gcaggtgtaa ctgcagtaac ttcatataat
     2461 tcatcaattg gaaacacatt aatgcaaggt tctcaggatt ctgttcaggt tactattcat
     2521 aactatggaa cagcagccct tacaagctta aatattactt atgctgtcaa tggaacaccg
     2581 gtgcttccac cttatgcatg gacaggcaac cttgctccta atggtaatgc tactattata
     2641 ttaccaacac catatacaac acctggtgga caatatacta ttactgcatg gactgactta
     2701 tcgggtgact gtaattccgc aaatgatact ttagcaggtg cagcagttgg agttccggta
     2761 attgttgttg actttaacaa ttcttattgt gatgattttg aaaatggaaa cataggatgg
     2821 tcaactaaga acaatagcac aacaccgggt tctgactggc aattcggaac accgacattc
     2881 ggaacaacaa attcggctta tagcggagta aactgttggg atattaacct gaatagtgtt
     2941 tatggtgcca atgccaactc agaactttat actccaatat ttgacctaac caatgcagta
     3001 gatgccacca ttagtttctg gcaaaatgtt gcatcagaga gcaattggga tggtacacgc
     3061 atggaatatc gtattggcaa tggagcatgg acaattctcg gaacaccatg cgatacttta
     3121 actatgggtc aatttaattg gtacaacgaa aatcaattga actctagtca gcaatatgca
     3181 tggactcaag gcttagctac ttgtccatta ccaacaggat ggatacgtag tatgcgtaag
     3241 ttagatccta tattcaacaa tcaaccaatg gttcagttcc gctatatctt tacttcagat
     3301 ggttctgttc agacaaatgg ttattctatt gacgacttct gtgttacagt accggttcct
     3361 ttaactgtaa ctcctgtagc tgtttcaact gcaaatccag gtcctccatt tattttccct
     3421 ggacagagta tgccatttaa gtctaagatt tataacgatg gtacttctgc tgtaaacaac
     3481 ttcgttacta cattaagtgt tgatggaaat attatctcta acgatactgt tacattaaca
     3541 acaccattaa atccaaacac caacattaat tacacttatg ttaacggttg gattgcatct
     3601 ccaggcttcc ataatgtatg tgtttctacc tacttcccaa atcagtctgc agatttaaaa
     3661 ccaattggtg ataccatctg ctattctgtt caggtgtttg actctgccac tgttggtgca
     3721 ggtgcatctt caaaatgtta tgattttgaa agcggctatc gttgggtaac tttaaattca
     3781 acatcttatt ctgcaaattc aagctgggat ttaggaacat ctgttaagtt aggtggaaat
     3841 cacagtcctg taaattcatg gtttactagc cttgcaggcc aatatccaaa tcgtgatact
     3901 tctgcattgt tctcacctgt atttacttta accggtggat tgacatacaa catcaatttc
     3961 tggcatagtt ttatgacaga aagaaatgaa gatggcggaa catttgaata ctctactaac
     4021 ttaggtgcaa catggactca gttaggtgac gatcatgatc caaactggat gaatacatac
     4081 agcatcactg cattaggacc tgttcctcct gtacatccgg gatggtcagg caacagcggt
     4141 ggttggggtc aggtaggaca tgatatttgc ttgcctactg caggaccaca acaagttatc
     4201 ttcagatgga gatttaattc tgacttcagt gttcgtgatg aaggttgggc aattgatgat
     4261 gtatgtttag aggtaaaaac tccgattaca ttatgtacta ccggtttgac agatctgaac
     4321 aacacgtttg atatgataca gaatacaccg aatccattta gtgattactc tgtaattaca
     4381 ttcaacttat cagacaacgg aagtacacaa cttgacattg ttgatgttac aggcaaagtt
     4441 cttatgacgc ctgttaacgc aaatctttct gcaggtactt acaacattac tgtaagtgca
     4501 gaaaatctgg ctagtggtgt ttatttttat gtattgaagc acaacgaccg tcaattagtt
     4561 aaaaagatgg ttattgctaa ataattttaa atacaattaa aaaaaagagg ctgtctccaa
     4621 aggacagcct cttttttttc tctcatcaca cagtataatt ttaatttttc atttctttgc
     4681 aacccgttca aacgaacatc tttaatcagg ggatgtagct cagttggcta gagcgcttgc
     4741 atggcatgca agaggtcgtg ggttcgagtc ccatcttctc cacaattaaa aattatcccg
     4801 ttacatgcgg gattttttat ttctttcaat agaaaattag ccttaaagtt aagtttgttc
     4861 aaactatcgc agccaatgat ttgtttaatt ttgcagtatg gaagaaaaag ctaaagagcg
     4921 attatataaa aagcccgaat ggttaaaagt aaaactacct attggcgaag aatacagaaa
     4981 agttcgaggc attgttgaca acaacaaact gcacacaata tgtgaaagtg gcaattgccc
     5041 aaacatgggt gaatgttggg gagcaggtac tgctacattt atgattcttg gaaacatctg
     5101 tacacgttct tgcaaatttt gtgctgttgc aactgggaaa cctttagcag ttgataaaac
     5161 tgaaccgcaa cgtgttgcag aatccgttaa gttgatgaaa gtaaaacatt gtgtaatcac
     5221 cagtgttgac cgtgatgatt tgaaagatgg cggttctgaa atttgggcag aaaccatcag
     5281 aaaggttaga gaaatttcag aaggaacaac aatggaaaca ttggttccgg actttgccgg
     5341 aaaatgggaa aatttagagc gagtgatgca ggaaagacct gagattatgt cgcacaattt
     5401 ggagacagta agaagactta caaaagaagt acgtgttcag gcaaaatacg acagaagtct
     5461 tgaagcctta aaacgaatca atgcttttgg attaagaaca aaatcaggtg tgatgttagg
     5521 tttaggcgaa acacgtgatg aagttttaga aactatgaat gacttatatc aaaacggagt
     5581 tcatatttta actttaggac aataccttca accaaccaag gaacatttac ctattgcaga
     5641 atttgttaat cctgctgttt ttaaagaata caaacaaata ggccttgaaa tgggttttaa
     5701 atatgttgaa agcgggccgc tagtacgctc cagctatcat gcagaaagac atttaacttt
     5761 tgatgtttaa ttataataaa ttatgacacg tgttgcaata aacggctttg gaagaattgg
     5821 ccgtacagtt ttaaaaaaat tacttctgaa taaaaacctg actattgtag gcatcaatga
     5881 tttgacagac ccaaaaacgt tagctcattt atttaaatac gatagtatcc atggcaagtt
     5941 tgatggaact gttacttctg cagaaaaagc aatcatcatc aataatcaaa ctattcctgt
     6001 ttatgcagaa aaagatcctg caatgttgcc atggaaagat ttacaggtag atgtagtgat
     6061 tgaatcaaca gggcgtttca caaaagctga attggcagaa gcccatataa aagcaggcgc
     6121 acgaaaagta ataatttctg cacccggatc aggaaattta aagtctgttg tgttgggcgt
     6181 gaatgaaaac atacttgacg gaagtgagaa aataatttcc aatgcaagtt gcaccacaaa
     6241 caatgcagca ccaatgataa aaatacttca ggacaattgg ggagtagaaa gtgcttacat
     6301 tactacagtg catgcttaca caggcgatca aaatatacac gatgctccac ataaagattt
     6361 acgaagggca cgtgctgctg cacaatccat tattcccaca acaacaggtg ctgctaaggc
     6421 agtgacagaa atttttcctg accttaaagg tatgattggt ggtgcaggaa tcagagttcc
     6481 ggtaatagat ggttcaataa cagatatcac ctgcattctt aaacacgatg ccactgttga
     6541 agctataaat caaaaatttt tggaagcatc gcaaggctct atgaaaggca ttattgaata
     6601 cacaaccgac ccaatagtgt ctgttgacat tatcggcaat acacattcat gcattt
//
LOCUS       DAOOOI010000101         6648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2252_length_6648_cov_15.453815, whole genome shotgun sequence.
ACCESSION   DAOOOI010000101 DAOOOI010000000
VERSION     DAOOOI010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2252_length_6648_cov_15.453815"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..590)
                     /locus_tag="PKX59_09725"
     CDS             complement(222..590)
                     /locus_tag="PKX59_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOZ91184.1"
                     /translation="MTLIKFKNEPASRMMDRSPMFSDLFNDFFDGVVSNNITKSFSPS
                     VNVKESDHEFKLELAAPGLSKDDFKINIENDMLNISAEKKEEQATQNEKYTHKEFSYS
                     SFKRTFTLIEKKFPQLMTTV"
     gene            893..1948
                     /locus_tag="PKX59_09730"
     CDS             893..1948
                     /locus_tag="PKX59_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ91185.1"
                     /translation="MKASRFILIVLIIAGLIAVKLIFFPGTKSSKFGGKANAAAVPVT
                     GFIARSQPLESNLKANGTINAYEQTDLRAEISGRIVAILFKDDQPVKKGQLLIQIYND
                     DLKANLSKSTAVLKQTQDRLKRQEKLLEIGGVSTEEVEETQTEAATQKAEIDYLNFQI
                     AKTEIYAPFDGIVGIRDISIGNYVNSSTVIATVYQVNPLKIDFTVPEKYLPYISKGMK
                     IRFTTDVNKKEYEGEISAINPMIDPSTRMVRLRAYTSNSDHSLTPGSFAHIQVDFKIT
                     ENAILIPTQSVIPILKGKKVFISKNGKADEKIIETGERTADMIEVTSGLTAGDTVLTT
                     GILQVRKGSDVKFSEVK"
     gene            1953..5018
                     /locus_tag="PKX59_09735"
     CDS             1953..5018
                     /locus_tag="PKX59_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOZ91186.1"
                     /translation="MDLSEISVKRPVLAIVMSLVILLFGVISYTFLGVREYPSIDPPI
                     INVRTSYPGANADIIESQITEPLEKVINGIQGIRSISSTSSQGTSNITVEFNLNSDLE
                     AAANDVRDKVSQAVRTLPRDIDGLPVVTKSDANADAIISLTVQSNTRNTLDLSDYAEN
                     VIGQQLITIPGVSTLQIWGQRKYAMRLRIYPDKLAAYKLTVQDIQNALNRENVELPAG
                     KIAGDNTELVVRTTGQLHSEDDFNNMIIFNDGDQLIRFKDVGLAELGPENEETILRES
                     GIPMVGIALVPQPGSNYIEIADEFYKRLEKIKVDLPSDIKIGIALDNTTFVKKSITEV
                     EETLLIAITLVVIIIYLFFRDWLVAIRPLIDIPVSLVGAMFIMYIAGFSINVLTLLAI
                     VLATGLVVDDGIVVTENIYKKIEAGLSPNKAAIEGTKEIFFAVVSTSVTLAAVFLPVV
                     FMEGFVGRLFREFGVVIAGAVLISAFVSLTLTPMLNARLVRKDKKHSKFYEKTEPFFQ
                     RMNQAYENSLKWFMEARWRAWTILGSAVALIFILGRSLPSELSPLEDRNWLRLLITAP
                     EGASYEYTDNFIVKISDVIEDSIPEKNGLLTVTAPGFTGAGAVNSGFARIVLTTPDKR
                     ERSQQEIADQLSGITRKMTDARVFVLQEQSLSAGGSRSALPVQFVLQNQEFDKLREVV
                     PAFLDKANKSPVFQGVDVNLKFNKPELNVSIDRAKANALGVSVDDIAQTLQLAFSGQR
                     FGYFIMQGKQYQVIGQVDRENRDVPYDLSKLYVKTNSGGLVQLDNLIEIKEISSPPQL
                     YHFNRYQSATISAGLAPGYTIGDGINEMKHIGDSLLDESFSTDLTGPSRDFAESSSNV
                     WFAFVLAIILIYLVLAAQFESFVDPFIIMITVPLAICGALISLWYFNQTLNIFSQIGI
                     IMLIGLVTKNGILIVEFINQLREKGVERSEAIIHGSVSRFRPILMTSLATSLGALPIA
                     LALGAGAKSRMSMGIVIVGGVLFSLIFTLYIIPAMYSVFSRKKEMVSYD"
     gene            5011..6309
                     /locus_tag="PKX59_09740"
     CDS             5011..6309
                     /locus_tag="PKX59_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOZ91187.1"
                     /translation="MIKVRLTVLICFFFVQAQCQQVFTLQQAIEKALNDNFSIRIARN
                     QSEILNNNATLGNAGMLPQVNLNGSYVKANNNVVQELSNGTSTEKQNAGQSTIAGSAV
                     LEWVLFDGLTMFSEYNKNSALSKQGKEQLKANIEDVIENVSKSYFTVVKEKLLLSSIN
                     ETLKFYADRLQIADSKYKIGSASKLDYLQSKVDYNAMLSQKLNKETDVNNAMVELNRL
                     LQYSADTLLQTEDTITFVTMPAMNDLITGLQQNTAMQVSKLNVEVNRLQLNSIKGLRL
                     PVITGLAGYYYNENRNDAGLFSLNRTNGPQFGITARYNLFNGFNNSRQISNTKLNYIN
                     SQIEFQQTRTNQIADLNNTWNAFQKSQEILNLETENTAAARENASVYLASYRLGSANL
                     LQLNQSQESLYDALVRLVNAQYVNKTTEIHLLRLAGKLIK"
     gene            complement(6317..>6648)
                     /locus_tag="PKX59_09745"
     CDS             complement(6317..>6648)
                     /locus_tag="PKX59_09745"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HOZ91188.1"
                     /translation="PSGSVFEDKFINGKGFDFAYNRGHLPNSTAVEDENEEAGFFKAG
                     DTIMVKFCTITEDSYKFWRQAESQAGSSGSPFGSPAVFPTNIKGGIGIFEAFNPTFYT
                     VIAKKPD"
BASE COUNT         2110 a         1086 c         1333 g         2119 t
ORIGIN      
        1 accattttgt tcgaaagtga tagtaaccat cgttttaatt ttattaaaat ggcagtaata
       61 cttgccggag ttggtctttc actgatggtt taaaataaaa tcctcttgca atttcttaca
      121 agaggattca ccactaaaat aaaccaaatt aaattatgaa atttttattt cgcgcaacgg
      181 ctttggcctt gcttcgtcct ttttaggaat tactaaggtc atcacaccgt tgtcataagc
      241 tgcggaaatt ttttctctat cagtgtgaaa gtgcgcttaa agcttgagta cgaaaattct
      301 ttgtgtgtgt acttctcgtt ttgtgttgct tgttcttctt tcttctctgc actgatgttc
      361 aacatatcat tttcaatgtt aatcttgaaa tcatccttgc tcaatccggg agctgcaagt
      421 tcaagcttaa attcatgatc agattccttt acgtttacag aagggctgaa tgatttagtg
      481 atgttgttgc taaccacacc atcaaagaag tcgttgaaca aatcactgaa cattggtgag
      541 cggtccatca ttcttgatgc cggttcgttt ttaaatttaa tgagtgtcat tttgtttttg
      601 ttttaattgt taatgtatgc agcatacatc aaattcaatt ccattacctt ttttatgaca
      661 atatcggcat aattgagtga ttttgtaagt cagattgtct atataatgca gtaactgacg
      721 acaatttttc cgatttattg aatagtgtaa tgttttttac tgtgtattaa ttctcaactg
      781 ataatgtact ttgctgaaaa ttttctaaag tttgttaatt gtttcaattg aaaacaatca
      841 attcataaaa tagaatcata tttttgttcc ttttatttct tttgattaat ttatgaaagc
      901 atcacgattt attttaatag tattaatcat tgcaggatta attgctgtta agttgatatt
      961 tttcccgggc accaaatctt caaagtttgg tggcaaagca aatgcagcag cagttcctgt
     1021 tacagggttt attgcccgtt cgcaacctct tgaaagtaat ttaaaggcaa atggaacaat
     1081 caatgcttat gagcagactg acttacgagc agagatttca ggacgcattg ttgcaatatt
     1141 gtttaaagat gatcaaccgg tcaagaaggg tcaactgctg atacagattt ataatgatga
     1201 tttgaaagcg aatctttcaa agagtacagc ggttttaaag caaacacagg acaggttaaa
     1261 acggcaggaa aaactattag aaataggagg ggttagcact gaagaggtag aagaaacaca
     1321 aaccgaagct gctactcaga aggctgaaat agattatttg aattttcaga ttgccaagac
     1381 agaaatttat gcgccatttg atggcattgt aggaatcaga gacatcagta taggcaacta
     1441 cgtaaattcg tcaaccgtta ttgctacagt ttatcaggtt aacccactta aaattgattt
     1501 tacagttccc gagaagtatc ttccttatat cagtaaagga atgaaaatca gatttactac
     1561 ggatgtgaat aaaaaggaat acgaaggtga aatttcagca attaacccaa tgattgatcc
     1621 gtcaacaagg atggttcgtc tgcgggctta cacatcaaac agtgaccatt cattaacacc
     1681 gggttctttt gcacacattc aggttgattt taaaatcact gaaaatgcaa tattgatacc
     1741 tactcagtct gtaattccaa ttttaaaagg caagaaagtt ttcattagta aaaatggcaa
     1801 ggcagacgaa aaaataattg agacaggtga gcgaacagca gatatgatag aagttacttc
     1861 aggtttgact gccggtgata cggtgttgac aacaggaatt ttgcaggtta gaaaaggaag
     1921 cgatgttaaa ttcagtgagg taaaataggt ttatggatct gagtgagata agtgtaaaac
     1981 gccccgttct tgctatagtt atgtcgttgg tgatactgtt attcggtgtc atcagttata
     2041 cttttcttgg tgtcagagag tatccatcta ttgacccacc aattattaat gtaagaacat
     2101 cgtatcccgg tgctaatgcc gatattattg agtcgcagat aacagaacca cttgaaaaag
     2161 tcataaacgg tattcagggc attcgttcaa tttcttcaac tagcagtcag ggaacaagta
     2221 atattactgt tgagtttaat ctgaattctg atctcgaagc agcagccaat gacgtaagag
     2281 ataaagtttc tcaagctgtt cgtactttgc cacgtgacat tgatggatta ccggttgtta
     2341 ctaaatctga tgctaatgcc gatgccatca tttcgcttac cgttcagagt aatacccgga
     2401 atactcttga cttaagtgac tatgcagaga atgttatcgg tcagcaatta attacaattc
     2461 caggtgttag caccttgcag atttggggac agcgtaaata cgctatgcgt ttaagaattt
     2521 atcctgataa acttgctgcg tataaactta ccgttcagga tattcaaaat gcattgaata
     2581 gggagaatgt ggaattgcct gcaggaaaaa tagcaggtga taatactgaa cttgttgtac
     2641 ggactacagg acagcttcac agcgaagacg atttcaataa tatgattatc tttaatgatg
     2701 gcgatcaact tattcggttt aaagatgtag gtcttgcaga attagggccg gagaatgaag
     2761 aaactattct aagggaatca ggcattccga tggtgggtat tgctcttgtg ccacaaccag
     2821 gaagtaatta cattgaaatt gctgatgaat tttataaaag gttggaaaaa ataaaagttg
     2881 atttgccttc tgatattaaa attggtattg cactcgacaa cacaactttt gtaaagaagt
     2941 caattaccga ggtggaagag acacttttaa ttgccattac gcttgttgta atcattatct
     3001 atttgttttt ccgtgactgg cttgtggcca ttcgtccatt gatagatatt ccagtctcgt
     3061 tggttggagc catgtttatt atgtacatag ctggattttc aattaatgtg ctaacgcttt
     3121 tagcgatagt gcttgcaacc ggattggtgg tagatgatgg aattgttgtt acagaaaata
     3181 tttataaaaa aattgaagca ggactttcac ccaacaaagc cgcaattgaa ggaacaaaag
     3241 aaatattttt tgcagttgtc agtacctcag taacgttagc agcagtattt ctacctgtag
     3301 tttttatgga aggattcgtt gggcgactct tcagggagtt tggtgtggtc attgcaggtg
     3361 cagtacttat ttcagcattt gtttcattaa ccttaacacc aatgttaaat gcgcgtttgg
     3421 tgagaaagga taaaaagcat tctaaatttt atgaaaagac agagccgttt tttcaacgga
     3481 tgaatcaggc ttatgaaaat tctcttaagt ggttcatgga agcaagatgg cgtgcatgga
     3541 caattttagg atcagccgtt gctttgattt ttattttagg aaggagtttg ccaagtgaac
     3601 tttcaccttt agaagacaga aactggctgc gacttttaat tacagcaccg gaaggagcgt
     3661 cttatgaata tacagataat tttatcgtga aaatttctga tgttattgaa gattctatac
     3721 cggagaaaaa cggattgcta acagtaactg caccgggttt tactggtgca ggagcagtaa
     3781 attcaggctt tgcccgaata gtacttacaa caccagataa acgtgaacgt tcgcaacaag
     3841 aaattgcaga tcaactttcg ggaattacac gcaagatgac tgatgcaaga gtatttgttc
     3901 tgcaggaaca gtcattgtcg gcaggaggat cacgtagtgc attaccagtt cagtttgttc
     3961 tccaaaatca ggaattcgat aagctaagag aggttgtgcc tgcattttta gataaagcaa
     4021 ataaaagtcc tgtatttcag ggtgtggatg tcaacctgaa attcaacaag ccagaattaa
     4081 atgtttctat tgacagagca aaggcaaatg cattaggggt atctgttgat gacattgcac
     4141 aaacacttca attagctttc agcggacagc gatttggata ttttatcatg cagggcaagc
     4201 agtatcaggt catcggtcag gtagatcgtg aaaatcgtga tgtgccttat gacctttcaa
     4261 aactttatgt aaaaacaaac agtggaggtc ttgttcagct tgacaattta attgaaataa
     4321 aagaaatcag tagtccacca cagttatatc attttaatcg ttatcaatct gccaccatat
     4381 cagcagggct tgcaccgggt tacacaattg gtgatggaat aaatgaaatg aaacatattg
     4441 gcgatagttt acttgatgaa agtttttcga ccgatttaac aggtccttca cgcgattttg
     4501 cagagagcag ttcaaatgtt tggtttgcat ttgtacttgc aattatttta atctatttgg
     4561 tgttggcagc acagttcgaa agttttgttg acccatttat tatcatgatt acagttccac
     4621 ttgcaatttg tggtgcattg atttcacttt ggtattttaa tcagacatta aatattttca
     4681 gtcagattgg tatcattatg ctcattggcc ttgttacaaa aaatggaatt ctaattgttg
     4741 agtttattaa tcaactacgt gaaaaaggag tagaacgatc tgaagcaatt attcatggtt
     4801 ctgtatcacg tttccggcca attttaatga caagtcttgc tacctcatta ggagcgctgc
     4861 ccattgcatt agctttagga gcgggagcaa agagtcgtat gtcaatggga attgtaattg
     4921 ttggtggagt acttttctct ctgatattta ctttgtatat tattccggca atgtattctg
     4981 ttttttcacg aaaaaaagaa atggtaagtt atgattaagg tacgattaac agtattgatt
     5041 tgttttttct ttgtacaggc acaatgtcaa caggtgttta cactgcaaca ggcaattgaa
     5101 aaggccctca atgataattt ttcaatccgt attgcccgaa atcagagtga aattctaaac
     5161 aacaatgcaa cattaggtaa tgcaggaatg ttgcctcagg taaatttgaa tggtagctat
     5221 gttaaggcaa ataacaatgt ggtgcaggaa ttgagtaatg gcacatctac agagaaacaa
     5281 aatgcaggtc agtcaacaat tgcaggtagt gcagttttgg aatgggtctt atttgatgga
     5341 cttactatgt tttcggagta taataaaaat tcagcactct caaaacaagg aaaagagcaa
     5401 ctgaaggcaa acattgaaga tgttattgag aatgtaagta aatcatattt tactgtagtt
     5461 aaagaaaagt tgttgttgtc gagcattaat gaaaccttga aattttatgc agacagactt
     5521 cagattgcag atagtaaata taaaattggt tcagcctcta aactggatta tttacagtca
     5581 aaagttgatt acaatgcaat gttatcgcag aagttgaata aggaaacaga cgtaaataat
     5641 gcaatggttg agttaaaccg tttgttacaa tattctgctg atactttatt gcaaactgaa
     5701 gatacaatca cttttgtaac tatgcctgca atgaatgatt tgataaccgg acttcaacag
     5761 aacactgcaa tgcaagtgag caaactgaat gtagaagtaa atcgtttaca gttaaattca
     5821 ataaagggat tacgtttgcc tgtaattaca ggtcttgcag ggtattatta taatgaaaac
     5881 cgcaacgatg caggattgtt ttctttgaac agaactaatg gaccacagtt tggaatcacg
     5941 gcacgttata atttgtttaa cggtttcaat aattcaagac aaatcagtaa tacaaaattg
     6001 aattatatca attcgcaaat tgaatttcag caaacacgaa ccaaccaaat tgctgattta
     6061 aataatacat ggaatgcatt tcaaaaatct caggagatac ttaatttaga aactgagaat
     6121 actgctgctg cccgtgaaaa tgcaagtgtg tatcttgcca gttatcgtct tggtagtgca
     6181 aatcttttgc aattaaatca atcacaagaa agtttatatg atgccttagt gcggttggtt
     6241 aatgcacagt atgttaacaa aactactgaa atacatttac tgcgtttggc aggcaaatta
     6301 attaaataga gaaaatttaa tcaggcttct tagcaattac tgtatagaag gtcgggttaa
     6361 aagcttcaaa aattccaata ccgcctttaa tatttgtagg aaaaactgct ggcgaaccaa
     6421 aaggactgcc actacttcct gcctgtgatt ctgcctgacg ccaaaactta tagctatctt
     6481 ctgttattgt gcagaatttt accataatgg tatcaccggc tttaaagaat cctgcttctt
     6541 cattctcatc ttcaaccgct gtactgttag gcaaatgtcc ccggttatat gcaaaatcaa
     6601 atccttttcc attaataaat ttatcttcaa acaccgagcc tgatggtg
//
LOCUS       DAOOOI010000102         6559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2295_length_6559_cov_12.208487, whole genome shotgun sequence.
ACCESSION   DAOOOI010000102 DAOOOI010000000
VERSION     DAOOOI010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2295_length_6559_cov_12.208487"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="PKX59_09750"
     CDS             <1..1258
                     /locus_tag="PKX59_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOZ91189.1"
                     /translation="PNFAAMKIFRSGWIYFVATFLFMAVGNIALAQGNKRELEKKRER
                     LQREINETNKLLKLTEKNKNATQAQLDALRKKISLRNDLINSINVEIKSLNGEIDKTG
                     KSIDSLERQMKQLREDYAAMIQFAQRNRNKYQTMMFVFAADDFNQAYKRLKFLKQFTE
                     NRRNQVEQIAEVQTSLNSKKADLQQKKNEKTGLKQTQESQRKNLESEKQEQDKMMENL
                     QASEKSLRKKLEAKIRDKAKLDKAIAAMVRKEIEAAKKKAVKAGKKNVTNENVFTLTP
                     EAQQLSNSFANNKGSLPWPVEKGRISETFGEHQHPLLKNVTTKNDGIDIRTSKGSSVR
                     SIFSGEVTGTVNLPNGSAVIIRHGEFLSVYSNLDEVFVTKGEKVTTKQNIGRVRTDSE
                     SGNADMNLQIWKGFTKLNPALWIAKK"
     gene            1321..1515
                     /locus_tag="PKX59_09755"
     CDS             1321..1515
                     /locus_tag="PKX59_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOZ91190.1"
                     /translation="MSSVTCLLLWSLGGSEILLIVLVLLLFFGGKKLPELARGLGKGI
                     KEFKDASSGVEKDEEKKENK"
     gene            1619..2581
                     /locus_tag="PKX59_09760"
     CDS             1619..2581
                     /locus_tag="PKX59_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ91191.1"
                     /translation="MKNLHLIIVAFWCLVSSCDNTSKNIPSIGFLDIAEDATLAKART
                     GFLDALSKNGFSEKDNTLKIYFSNAQGEIAVLNQACDYLISKNVKLIAANSTLSTIAA
                     VQHSKDIPVCMMVAPRPDLAGLADAQGNYPKNLFGVFETLAYIDSSVALIKRFFPDAV
                     NVGTIYNSSEPQSRDALEELQKAATTSGMKIIALPVTNSSETQLVTQSLIAKKPDVFF
                     ALPDNVIFASFETVVKACNEAQLPVFTSEAGLVSRGAIASYGADFYQWGFQAGVEAAN
                     YLKSNTATIPKPELVQVRQYVFNNKAATRFNLVAPNGFIVIDSH"
     gene            2581..3432
                     /locus_tag="PKX59_09765"
     CDS             2581..3432
                     /locus_tag="PKX59_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ91192.1"
                     /translation="MEVIIPAIIQGLCYAAMAFGIYLTLRIFNIPDITTDGSYTLGGV
                     VAAILISNHWHPAVALPIVLAAGALAGVATGLIHTQLKLHPLLAGILVMTSLYSINLF
                     IMGRPNIPLINEASIFNGSTSFNMLLAAMFSIILLLLLFWLMKTDFGIAMRATGSNEL
                     MVKAMGININLMKIAGLAIANALTALSGYLMVQYQGFADINMGIGIVIAGLAAVILGE
                     SFAVFFKSSSIFSALITVLLGSVLFRLLLAFALLAGVPPQMLKLLTAVLVLLAAGVFK
                     FIFKPAQ"
     gene            3429..4175
                     /locus_tag="PKX59_09770"
     CDS             3429..4175
                     /locus_tag="PKX59_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ91193.1"
                     /translation="MIEVSQISKKFFDGEKPVIALQPTSLQIKVKEFVIIVGANGSGK
                     STLLNIIAGTILCDAGKIAVNGKDITALKEHERSRFIARVFQNPQSGTASDLTIIENF
                     RLAALRSHSKKLSIGIDKKFVDFIRERVSWLGLGLEDQLNRKMGALSGGQRQALTVVM
                     STMDDFKLLLMDEPTAALDPKSAELIMQLTTRILSQMPVSVLMVTHQTKQVIDYGNRV
                     IQMSSGQIIRDLDKASKEKLTLNEIIHWFD"
     gene            4310..6241
                     /gene="dxs"
                     /locus_tag="PKX59_09775"
     CDS             4310..6241
                     /gene="dxs"
                     /locus_tag="PKX59_09775"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOZ91194.1"
                     /translation="MEIKAGELLSSINTPDDLRKIKPEQLEQVCKELRQFIIDIVSVN
                     GGHFGASLGVVELTVALHYVFNTPNDQLIWDVGHQAYGHKILTGRRDVFHTNRIYKGI
                     SGFPKRSESVYDTFGVGHSSTSISAALGMSVASRLKDDKNRQHIAVIGDGAMTAGMAF
                     EALNHGGVENSNLLVVLNDNCMSIDPNVGALKEYLTDITTSPTYNKVKDEIWKALGVI
                     SKFGPNAQAIAQKIENAMKSAVLRQSNLFESLRFRYFGPIDGHDVNHLTKVLEDLKNI
                     PGPKILHCLTVKGKGFKLAEQDQTKWHAPGLFDKITGEIFKPQNNTPQPPKYQDVFGH
                     TMVELAEKNSKIVGVTPAMPSGCSLNIMMKAMPDRAFDVGIAEQHAVTFSAGMATQGL
                     IPFCNIYSSFMQRAYDQVVHDVALQKLNVVFCLDRGGLAGADGPTHHGAFDIAYMRCI
                     PNMIVSAPMNEEELRNLMYTAQHDNLGPFCIRYPRGNGVLTEWRTPFKAIPVGTGRKL
                     RGGEQVAILTFGHPGNFAVAACDELAKENIYPAHYDLRFVKPLDQMLLHEVFANFKKI
                     ITVEDGCLQGGMGSAVAEFMIDNGYAAEIVRLGIPDQFIEHGEQSELYAECHFDTQSI
                     MKTVRQLIAASHPGIKSLK"
BASE COUNT         2067 a         1143 c         1370 g         1979 t
ORIGIN      
        1 acctaatttt gctgcaatga aaattttccg ttccgggtgg atttattttg ttgctacatt
       61 cttgtttatg gctgttggca atattgcctt agcacaaggt aacaagcgcg aactggagaa
      121 gaaaagagag cgccttcagc gtgaaattaa tgagactaac aagctgctga aacttaccga
      181 gaaaaataaa aatgccacac aggcacaact tgatgccttg agaaaaaaaa tatctctccg
      241 taatgacctc atcaactcaa ttaacgttga aattaaatcg ctcaatggtg agattgataa
      301 aacaggaaaa tctatagatt ctcttgaaag acaaatgaag caattgcgcg aagattatgc
      361 agcaatgatt cagtttgcac aacgcaacag aaataaatat cagaccatga tgtttgtttt
      421 tgctgctgac gattttaacc aggcttacaa gcgacttaaa tttttaaagc agtttactga
      481 gaacagacga aatcaggttg aacagattgc agaagtccag accagcctga acagtaaaaa
      541 ggctgacctg cagcaaaaga aaaatgaaaa gacgggactc aaacaaacac aggaaagcca
      601 aaggaaaaat cttgaaagtg aaaagcagga gcaggataaa atgatggaaa acctgcaggc
      661 aagcgaaaaa tcattacgca aaaagcttga agcaaaaatc agagataaag caaagttaga
      721 taaggctatt gctgcaatgg tgagaaaaga aattgaggca gcaaagaaaa aagcagtgaa
      781 agcaggtaag aagaacgtta ctaatgagaa tgtatttaca ctaacaccgg aagcgcagca
      841 attaagcaac agctttgcca ataacaaggg cagtttgcca tggcctgttg aaaaagggag
      901 aatatctgag acgtttggcg aacatcagca cccattatta aaaaatgtga caactaagaa
      961 tgatggaatt gatatccgta catcaaaggg cagctcagta cgaagtattt ttagtggaga
     1021 agttacaggc acagtcaatt tgccgaatgg ctcggcagta attatcaggc atggcgaatt
     1081 tttatctgtt tattccaatc ttgacgaagt gtttgtcact aaaggtgaga aggttacaac
     1141 aaaacaaaac attggtcgtg taaggactga ttctgaatca ggtaatgctg atatgaatct
     1201 tcagatatgg aaaggtttta ccaaactaaa tcctgcattg tggattgcaa aaaaataatt
     1261 taacgattat ttgcatgtaa cattataatt tcttgtatat ttgtaccata tatttaaaga
     1321 atgagttctg ttacttgttt actgctttgg agtttaggtg ggtctgaaat actgctgatt
     1381 gttttagtgt tgttgttgtt tttcggagga aaaaaattac ctgaattggc aagaggactt
     1441 ggtaaaggga taaaagaatt taaagatgca tcatccggtg ttgaaaagga tgaagaaaaa
     1501 aaagaaaata aataaacaac gatagttttc atgacttcgg gacgtttgcg caagcagcgt
     1561 cccgtttttc atttctgata gtttagattt tcttagtttt ataccttcat tgttcattat
     1621 gaaaaacctg catttaatca ttgttgcatt ttggtgctta gtttcatcgt gcgataacac
     1681 ttcaaaaaat attccgtcaa ttggattttt agatattgca gaagatgcaa ctttagcaaa
     1741 ggcacgaaca ggatttttag acgcattgtc aaagaatggc ttttcagaaa aagataatac
     1801 tctaaaaatt tatttttcaa acgcacaggg tgaaattgct gttttgaatc aggcatgtga
     1861 ttatttgatt tcaaaaaatg taaaactcat tgcagccaat tcaacgcttt caacaatagc
     1921 ggcagtgcag cattcaaaag atattcctgt ttgtatgatg gtagcaccac gccctgatct
     1981 tgcaggactc gcagatgccc aaggaaatta tcctaaaaat ctctttggtg tttttgaaac
     2041 attggcatat attgacagct cagtagcatt gataaaacgt ttctttcctg atgcagtgaa
     2101 tgttggcacc atttacaatt ccagcgaacc acaatcgcgt gatgcgcttg aagaattaca
     2161 aaaagcagca acaacatcgg ggatgaaaat tattgccttg ccggttacaa attcttcaga
     2221 aacacaactg gtaacacaat ctctcattgc aaaaaagcct gatgtgtttt ttgcgctccc
     2281 cgacaatgtc atttttgcat cattcgaaac ggtggttaag gcatgtaacg aagcacaact
     2341 tccggttttt accagcgaag ctggcttggt tagtcgtgga gctattgcat cctatggtgc
     2401 cgatttttat caatggggct ttcaggcagg tgtggaagca gcaaattatc ttaaatcaaa
     2461 cactgcgaca atccccaaac cggaacttgt tcaagtcagg caatatgtat tcaacaataa
     2521 agcagcaact cgtttcaatc ttgttgcacc caatggtttt atagtaattg attcacatta
     2581 atggaagtaa ttattcctgc aattattcag ggcttgtgct atgctgctat ggcatttggc
     2641 atttatctga cattgcggat ttttaatata ccggacatta ccaccgatgg cagttataca
     2701 ttaggcggtg ttgttgctgc aattttaatt tcaaatcatt ggcatcctgc tgtagcattg
     2761 cccatagtac ttgctgcagg tgccttggca ggagttgcaa caggtctcat tcacacacaa
     2821 ctgaagttgc atccgttgct tgcaggtatt ttagtaatga catcactcta ttccatcaac
     2881 ctgtttatta tgggaagacc taacattcca ttaatcaatg aagcaagtat ttttaatggg
     2941 agcacaagtt ttaatatgtt actggcagca atgttttcaa ttattttatt gttgttatta
     3001 ttctggctga tgaaaaccga tttcggcatt gcaatgcgtg caacaggcag caatgagtta
     3061 atggttaaag cgatgggcat aaatattaat ttaatgaaaa ttgcaggatt ggcaatagcc
     3121 aatgcactga cagcattaag tggttattta atggtgcaat atcagggatt tgctgatatt
     3181 aatatgggta ttggaattgt aatagccggt ttagcggctg ttattctggg cgaatcgttt
     3241 gcagtttttt tcaaatcatc ctctatattt agtgcattaa taactgtctt attaggctct
     3301 gttttatttc gattgctttt ggcatttgca ttacttgccg gtgtgcctcc gcaaatgctt
     3361 aaattactta cagcagttct tgtgttgctg gcagcaggag tttttaaatt tatttttaag
     3421 cctgcacaat gattgaagtt tcgcaaataa gtaaaaagtt ttttgatggt gaaaagcctg
     3481 tcattgcttt gcaacctaca tcactacaaa ttaaagtaaa agaatttgtg attattgttg
     3541 gtgccaatgg ctccggaaaa tctacgttgc tcaatattat tgcaggaact atattatgcg
     3601 atgcaggtaa aattgctgtt aatggcaagg acattacggc acttaaagaa catgaaagaa
     3661 gtcgttttat tgccagagtt tttcaaaacc cgcaatcggg tactgcttca gatcttacaa
     3721 ttattgaaaa cttccggtta gcggctttgc gaagtcattc aaagaagtta agtataggca
     3781 ttgataaaaa atttgttgat ttcatcagag aaagagtttc ttggctggga ctaggtctcg
     3841 aagatcagtt aaaccgaaag atgggagcac tgtccggagg gcaacgtcag gcactgacag
     3901 tagtgatgtc aacaatggac gattttaaac ttctgctaat ggatgaaccc acggcagctc
     3961 ttgaccctaa aagtgcagaa ctcattatgc agctaactac acgaatttta agtcaaatgc
     4021 ctgtttcagt actaatggtt acccatcaga caaaacaggt tattgattac ggaaacagag
     4081 ttattcagat gtcatcagga cagataataa gagatttaga taaagcatca aaggagaaac
     4141 ttaccctgaa tgaaattata cattggtttg attaagcaga atagtccggt aataaaaaat
     4201 cgtctttagc gtagtgtttt tcaacctttc gccaaattat atgttgtttt cattggtaat
     4261 ttggggtagt tccattcatt atttgtaagt ttgcatcact atttgctgta tggaaattaa
     4321 agcaggagag cttcttagta gtattaacac acctgacgat ttaaggaaaa ttaaaccgga
     4381 gcagcttgag caggtttgta aggagttacg acagtttatt attgatattg tttctgttaa
     4441 cggaggacac tttggcgcaa gccttggtgt ggttgaatta acggttgcac ttcactatgt
     4501 ttttaataca cccaacgatc aattgatatg ggatgtaggt catcaggcct atggtcataa
     4561 aatcctgacc ggcagacgcg atgtttttca taccaacaga atctataaag gtattagcgg
     4621 ttttccaaaa cgcagcgaaa gtgtttacga tacctttggt gtaggtcata gctcaacttc
     4681 aatttctgct gcgcttggaa tgagtgttgc aagcagatta aaagatgata aaaaccgtca
     4741 gcacattgct gtaattggtg atggtgccat gacagcaggt atggcatttg aagcacttaa
     4801 tcatggtggt gtagaaaaca gtaaccttct agttgtgctt aacgacaact gcatgagcat
     4861 tgaccccaat gttggtgcac tgaaagaata ccttactgac attaccacat caccaacata
     4921 taataaagtt aaagacgaaa tctggaaagc attaggtgtc atcagtaagt ttggtcccaa
     4981 tgcacaagcc attgctcaaa aaatagagaa tgcaatgaaa tcggctgtgc ttagacaaag
     5041 caacttattc gaatcgcttc gctttcgtta tttcgggcca atagatgggc atgatgtgaa
     5101 ccatctgacc aaagtacttg aagatttaaa aaatattccc ggccctaaaa ttttgcactg
     5161 tcttacagta aaaggaaaag gatttaagct tgcagagcaa gatcaaacca aatggcatgc
     5221 accggggttg tttgataaaa taaccggaga gatttttaag ccgcaaaaca acacaccgca
     5281 accaccaaaa tatcaggatg tatttggcca tacaatggtg gaacttgcag aaaaaaattc
     5341 aaaaattgtt ggtgtaacac ctgctatgcc atcggggtgt tcattaaata ttatgatgaa
     5401 agcaatgcct gatagagcat ttgatgtggg tattgccgaa caacatgctg taacatttag
     5461 tgcaggcatg gccacacaag gtcttattcc gttttgtaat atttattcct catttatgca
     5521 gcgtgcctac gatcaggtag tgcatgatgt tgctcttcag aaattaaatg ttgttttctg
     5581 tctcgacaga ggaggccttg ccggtgctga cgggcctaca catcatggtg catttgatat
     5641 tgcctatatg cgatgcatac ctaatatgat tgttagcgct ccgatgaatg aagaagagtt
     5701 acgcaatctg atgtacactg cacaacatga taacttagga ccattttgta ttcgttatcc
     5761 gagaggtaat ggtgtgttga cagaatggcg tacacctttc aaagcaattc ctgtaggaac
     5821 aggtcgcaaa ttaagaggtg gagaacaagt tgctatttta accttcggac atccgggtaa
     5881 ttttgcagta gcagcatgcg atgagcttgc taaagaaaat atttatcctg cacattacga
     5941 tttgcgtttt gtgaaaccac ttgaccaaat gttgcttcac gaagtctttg ctaattttaa
     6001 aaagataatt actgttgaag atggttgtct gcaaggagga atgggaagtg cagttgcaga
     6061 gtttatgatt gataatggct atgctgcaga aattgtcaga ctgggaattc ccgatcagtt
     6121 tattgagcat ggtgaacagt ctgaattata tgcagagtgt catttcgata cgcaatcaat
     6181 aatgaaaact gttagacagt taattgctgc ctcacatccc ggtattaaat ctctcaagta
     6241 attttttttt ctgatagttg agaaagccat tcaatgtttc ttgttgaagt atgtcgaatt
     6301 ttattttagt catcatagtt atctgtatga tgtaaataaa gaaacccgac ttctcaagtc
     6361 gggtttcggg gggacataca caatgaataa tttaattact catcgacttc tgtttctgtt
     6421 gcattgccgg gaaccgtttt tactttttcc cattggcctg tgccccagtt atatttttct
     6481 atttcatatc gttcaactcc tgtatcccat ttatcatatt tagtccacca cagtctgctg
     6541 tttgcatact cccatttcg
//
LOCUS       DAOOOI010000103         6546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2301_length_6546_cov_13.823294, whole genome shotgun sequence.
ACCESSION   DAOOOI010000103 DAOOOI010000000
VERSION     DAOOOI010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2301_length_6546_cov_13.823294"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PKX59_09780"
     CDS             <1..955
                     /locus_tag="PKX59_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOZ91195.1"
                     /translation="ALSFFITNETNLLSKYLFEKYYITNNESHNLSEKLEFNKQQGGI
                     NPDIAFPFLNLLSEEIDKEVFTASMFQQPLVWIRIRKGFEQHVSDELKSKEITPLQIE
                     GKTLGFAPAAPLTTLESYLKGYFEIQDLSSQQTLSLLPDNCGQRMWDCCSGSGGKTLL
                     LADRFPNASFFLTDIRSSSLQNAETRLKKATVRNYKTLQADLTSDLNFNEKFDVIIAD
                     VPCSGSGTWCRTPENFWNFDKHKIKDYAELQFTIAKQATMHLKSNGLFLYLTCSAFAE
                     ENEENTKRIAKHCNLSIVVQKFFHGYSLQADTMYGCVMMGF"
     gene            1096..3477
                     /locus_tag="PKX59_09785"
     CDS             1096..3477
                     /locus_tag="PKX59_09785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HOZ91196.1"
                     /translation="MKKFFTLFLAACFATAVNAQNFNISLLGTLPYPNDDLANIGGWV
                     SPSGEEYALVGSESGLSIVDVSNPANPTEVILVPGPQSQWREVKTYDHYAYVTTEGGG
                     GLQIVDLSQLPNAAPAIDWTGNGAISGQLDNIHSLHIEDGYVYLHGSNLFQGATIIAD
                     IATNPLSPNYVGHTPGAYVHDGYARGNYYYGCHIYNGDFTIYDVTNKANPIPLGIKVT
                     PGQFTHNAWLSTNSQYLFTTDEVSDSYLTAYDISNPANIIEMDRIQITPGSGSIVHNT
                     HIIQKNGADYAVTSWYKDGVVITDVTRPQNMVNVGWYDTYTQGAGDGFSGDWGVYPYL
                     PSGNLVVSDINNGLFVLGPTYQRACYLEGVVTDSVTGNPIFNATVKVLSTNITKNTKA
                     NGEYKTGTATAGSYNVEVSAAGYFTKTINGVSLTNGNVTQLDVQLSPLASTSLSGQVV
                     EAVTGNPIPNAMVTIQDSALVYNTTADASGNFTIPSMVIGNYQVVAGSWGHRTTCFNS
                     SIMLNPAPLVITLNKGYYDDFSFDFGWTITGTTQNLWERGVPHGTPAVFGPGFANPDA
                     DDQTDCLDQAYVTDNNPNASSSGSNDVDDGYTLLTSPVFDPTYLTNPTINYVRWFYNA
                     GGNSNPNDSLIIRVSNGTTNVVLETVRASTPGMGTWVPQSFNLTGLIPITSTMQISVY
                     TADQAMSGHVVEGGFDKFEVTGTVGISTLISATNTLSSSPNPFNNESRLTIGENGGTL
                     TITDITGKMVETLKVHKNEQILIGKNFQKGIYLVKLVSDQGVISSIKLVKTGE"
     gene            3651..3962
                     /gene="rplU"
                     /locus_tag="PKX59_09790"
     CDS             3651..3962
                     /gene="rplU"
                     /locus_tag="PKX59_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOZ91197.1"
                     /translation="MYAIVNILGQQFKVEEKQQIFVHRLEGAEGEEVKFNDVLLVDNG
                     GNIKVGKPSLSNASVSAKIISHVKGDKVLVFKKKRRKGYKKMNGHRQYMSKIEINSIN
                     A"
     gene            3983..4246
                     /gene="rpmA"
                     /locus_tag="PKX59_09795"
     CDS             3983..4246
                     /gene="rpmA"
                     /locus_tag="PKX59_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009090203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOZ91198.1"
                     /translation="MAHKKGEGKVKNNRESHSKRLGIKIFGGQHAIAGNIIVRQRGTK
                     HNAGEGVGMGKDHTLFALVDGKVVFKKKTDNKSYVYITPVAQA"
     gene            4339..5391
                     /gene="lpxK"
                     /locus_tag="PKX59_09800"
     CDS             4339..5391
                     /gene="lpxK"
                     /locus_tag="PKX59_09800"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOZ91199.1"
                     /translation="MKSLRMLLLPFSWLYGMVVMLRNFLFDAGLLKSRTVNLPVICIG
                     NLNTGGTGKTPHTEYLLSALSNYKTAVVSRGYGRNTSGIAEVLVDSQANDTGDEPLQI
                     KRNFPTVPFIVSEKRNDGIDYLLKHHPNTEVILLDDAFQHRSVKAGMNLLLTQYNDLF
                     TNDCLLPAGNLREPALSASRADVIIVTKCPSTITAEQKKSIISELPKKPVFFSSFNYD
                     NLQSLNSNEKLNLTDLYEYEILLITGIASNKNLLDFLSTHSRKIKEISFKDHHNYCAK
                     DFENITAIFNTLASEKKIIVVTEKDAVKFNSSELKPLTNNLPVYSIPVTVVINENEIK
                     FLDIVKSYVGKNQSNG"
     gene            5363..6172
                     /locus_tag="PKX59_09805"
     CDS             5363..6172
                     /locus_tag="PKX59_09805"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOZ91200.1"
                     /translation="MLEKIKATAEFISKTTGFSPEIGIILGSGLGGLVKEIEIVKELN
                     YSDIPGFPVSTVKGHGNKLIFGTLNGVKVVAMQGRFHFYEGYSMQEVAMPVRVMKFLG
                     IKNLILSNASGGVNPKYKVGDLMILDDHINLMGTNPLIGPNNDDLGPRFPDMGEPYDK
                     TMIKNAKAIADKHGFTCHTGIYAAVTGPTFETRAEYKYIKFIGADCVGMSTVPENIVA
                     RHMNLPVFAISIITDLGGSDEPEFITHEEVLKVANAAEQKMTVIIKELIAQ"
     gene            6271..>6546
                     /locus_tag="PKX59_09810"
     CDS             6271..>6546
                     /locus_tag="PKX59_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91201.1"
                     /translation="MQKVQAQFWLPIGSGVNDNVYAMTKDTVNNILYIGGRFTDASGV
                     TAIRIASWNGTTYTAIGNGFDSNVFALYYDHSNNTLYAAGSFLFSGIN"
BASE COUNT         2190 a         1195 c         1211 g         1950 t
ORIGIN      
        1 ggctttgagt ttctttataa ccaatgaaac aaacctgtta agcaaatatt tattcgaaaa
       61 atattatatc acaaataacg aatcacataa tctttctgaa aaactggaat ttaataaaca
      121 acagggagga ataaatccag atatagcttt tccgttctta aatttacttt ccgaagaaat
      181 agataaagaa gtatttactg catcaatgtt tcaacaacct ttagtgtgga taagaatacg
      241 aaaaggtttt gagcaacatg tttcagatga attgaagtca aaagaaatta ccccattgca
      301 aattgaagga aaaacgttgg gctttgcccc tgctgcccct cttacaacac ttgaatcata
      361 cctgaaaggc tattttgaga ttcaagattt atcatcacaa caaacactat cgttgctacc
      421 cgataactgc gggcaacgta tgtgggattg ttgcagtggc tcaggtggca aaactttgtt
      481 actggccgac cgttttccaa atgcgtcttt ttttcttacc gacatacgca gttcttcact
      541 tcaaaatgca gaaacaagat taaaaaaagc tactgtcaga aattataaaa cattgcaagc
      601 cgaccttaca tcagacttaa actttaatga aaaatttgat gtgattattg ctgatgtgcc
      661 atgcagtggc tccggcacgt ggtgtcgcac acctgaaaac ttttggaatt tcgataaaca
      721 taaaattaag gactatgccg agttgcaatt taccattgcc aaacaagcta caatgcattt
      781 aaaaagtaac ggcctgtttc tgtatttaac atgctctgca tttgccgaag aaaatgaaga
      841 aaataccaaa cgtattgcaa aacattgcaa tcttagtatt gtggtgcaaa aattttttca
      901 tggatacagc ctacaagcag atacaatgta tggttgtgtg atgatgggat tttgatagaa
      961 attatcgtaa ttatcaccta aaaactcatt tatcccccca cccattcacc tgttaattga
     1021 tgttaaatta aaattgcatc acactactga tgtattgcta ttttgatagc tttgtcggtc
     1081 ttttcaaaaa taaacatgaa aaaattcttc acactctttt tagctgcatg ctttgctaca
     1141 gcagttaatg cacaaaattt caacatttct ttacttggca cattgcccta ccccaatgac
     1201 gaccttgcca atattggcgg atgggtatca ccatcgggcg aagaatatgc actcgtaggt
     1261 tcagaatccg gactttctat tgttgatgtc agcaatcctg caaacccaac agaggtaatt
     1321 ttagttcccg gaccacaatc gcagtggcgc gaggtaaaaa cttacgatca ttatgcttat
     1381 gtaacaactg aaggtggtgg cggactacaa attgttgacc tctctcaact tcctaatgca
     1441 gcacctgcaa ttgactggac aggtaatggt gccatcagtg gtcagttaga taacattcac
     1501 tcattgcata ttgaagatgg ctatgtttat ctgcatggct caaatttatt tcagggtgca
     1561 actatcattg cagatattgc aaccaatcca ctaagtccaa attatgttgg acatacacca
     1621 ggtgcctatg tgcatgacgg ctatgccaga ggtaactatt attacggttg ccatatttat
     1681 aatggcgatt ttaccattta tgatgttacc aataaagcca atcccattcc attgggaata
     1741 aaagtaacac cgggacagtt tacacacaat gcatggcttt ctaccaacag tcagtatttg
     1801 tttactactg atgaagtttc tgattcctac ttgacagcct atgacatttc aaatcctgcc
     1861 aacattattg aaatggacag aattcaaatc actcccggct caggttctat cgttcacaac
     1921 acgcacatca ttcaaaaaaa tggtgctgac tatgcagtta cttcatggta taaagatggt
     1981 gtggtgatta ctgatgtaac acgtccacaa aatatggtta acgttggttg gtacgatacc
     2041 tatacacaag gtgcaggtga tggtttcagt ggcgattggg gtgtttatcc ctatctgccc
     2101 tcaggaaatc ttgtagtatc tgacatcaac aacggacttt ttgttttagg acctacctat
     2161 cagcgtgcct gctatcttga aggtgtggtg acagattcag ttacaggtaa ccctattttt
     2221 aatgcaacgg taaaagttct atctacaaac attactaaaa acacaaaagc aaacggagaa
     2281 tataaaacag gaacagctac tgcaggtagt tataatgttg aagtttctgc agcaggttat
     2341 tttaccaaga caattaacgg tgtttcgctc actaatggaa atgtaacaca acttgatgtt
     2401 cagttgtcac cacttgcttc tacatcacta agcggacagg tagttgaagc tgtaacagga
     2461 aacccaatac ccaatgcaat ggtcaccatt caggatagcg cattggtgta taatacaaca
     2521 gccgatgcct caggcaattt tacaattcct tcaatggtaa ttggcaacta tcaggttgta
     2581 gccggaagtt ggggacatcg cactacatgt ttcaactcaa gcattatgct taaccctgca
     2641 ccattggtta taactttgaa caaaggttat tatgatgact tttcttttga ctttggctgg
     2701 acaataacag gcactactca aaatctttgg gaaagaggcg taccacatgg aacacctgca
     2761 gtattcggac cgggctttgc taatcctgat gctgacgatc agacagactg tttggatcag
     2821 gcttatgtta ccgataacaa tcctaatgca tcttcttcag gatcaaacga tgttgatgat
     2881 ggctatacat tattgacatc tcctgttttt gacccaactt atctcaccaa tccaaccata
     2941 aattatgttc gctggtttta caatgccggt ggaaattcaa atccaaatga cagtttaatt
     3001 attcgtgttt caaatggaac taccaatgta gttttagaaa cagttcgtgc ttctactccc
     3061 ggaatgggaa catgggtgcc acagtcattc aacttaactg gtttaattcc tattaccagc
     3121 accatgcaga ttagtgttta cactgccgat caggcaatgt caggacatgt ggttgaaggc
     3181 ggttttgata aatttgaagt aacaggaaca gttggcattt caacactaat ttcggcaaca
     3241 aacactttgt cgtcaagccc taatcctttt aacaatgaat ctaggttgac aattggcgaa
     3301 aatggtggca ctttgaccat tacagatatt accggcaaaa tggtggaaac gctaaaagtt
     3361 cataaaaatg aacagatttt aattggcaag aacttccaaa aaggcattta tcttgtgaaa
     3421 ttggtatctg atcagggtgt tatttcaagt attaagttag ttaaaaccgg agagtaaatt
     3481 tttatattat attgataatc aaatattttt attctattaa tagagtataa tcagcgtcat
     3541 tttccggccg attaaccaaa ttattgaagt tgaaaataaa tttaataaag gatttgaaaa
     3601 tatagtaaaa aaaactactt ttgccgtccg tttgtaaaaa cacattaatc atgtacgcaa
     3661 tagtaaacat attaggacaa cagtttaaag ttgaagaaaa gcagcaaata tttgtacacc
     3721 gtttagaagg tgctgaaggc gaagaagtaa aattcaacga tgtattatta gttgataatg
     3781 gtggcaacat aaaagttggc aagccttctt tatcaaacgc aagtgtatct gcaaaaatca
     3841 tcagtcatgt aaaaggtgac aaagttctgg tgtttaagaa aaagagaaga aaaggatata
     3901 agaagatgaa tggtcatcgt cagtatatga gtaaaattga aatcaattct attaacgcat
     3961 aaaaaactaa acatatttag tcatggcaca taaaaaaggc gaaggtaaag taaagaacaa
     4021 ccgcgagtcg cacagtaaac gtctcggaat taaaatattt ggtggacaac atgctattgc
     4081 aggcaacatc attgttcgtc agcgtggcac caagcataat gcaggtgaag gtgtaggtat
     4141 gggaaaagat catactttgt ttgcattggt tgatggcaaa gttgttttca agaaaaaaac
     4201 tgataacaaa tcatacgtgt acattacccc tgtagcacag gcttaattta aaatattaaa
     4261 ttgttaaaag cctacccttt aacacgggta ggctttctta tttttataaa atttcttttc
     4321 tatcaatact tttgttcaat gaagtcattg cgcatgttac tccttccttt ctcgtggctg
     4381 tacggaatgg ttgtaatgtt gcgtaatttt ctatttgatg ccggattact aaaaagcagg
     4441 actgtaaatc tgcctgtaat atgcattgga aacctaaaca caggtggcac aggcaaaaca
     4501 ccacatacag aatatttact ttctgcattg agtaattaca aaacagccgt tgtttctcgc
     4561 ggatatggac gtaacacatc aggcattgcc gaagttctgg ttgacagtca agccaatgac
     4621 accggtgatg aaccattaca gattaaacgc aattttccaa ctgtaccttt cattgtcagt
     4681 gaaaaaagaa atgatggcat tgattatctt ttaaaacatc atccaaatac agaagtaatc
     4741 ttacttgacg atgcctttca gcatcgcagt gtaaaagccg gaatgaattt attgctgaca
     4801 caatacaatg atctgtttac aaatgattgc ttattacctg caggtaatct tcgggaaccg
     4861 gcattgtctg catcaagagc agatgtaatt atagttacca aatgcccatc aacaataaca
     4921 gcagaacaaa aaaaatcaat catcagtgaa ttacctaaaa aacctgtctt ctttagttca
     4981 tttaactatg acaatcttca gtcgttaaat agtaatgaaa aactgaattt aacagacctg
     5041 tatgagtatg aaattttgtt aataacggga attgcatcca ataaaaattt actcgatttt
     5101 ctgagtactc atagcagaaa aatcaaagaa atatccttta aagaccatca caattattgt
     5161 gcaaaagatt ttgaaaatat cactgccatt tttaatactt tagcctctga aaaaaaaata
     5221 attgtagtta cagaaaaaga tgctgtaaaa ttcaacagca gtgaattaaa accactcaca
     5281 aataatcttc ctgtatattc aatacctgta acagttgtca tcaatgaaaa cgaaataaaa
     5341 tttttagata tagtaaaatc gtatgttgga aaaaatcaaa gcaacggctg aatttatcag
     5401 caaaaccacc ggctttagtc cggaaatagg aataatttta ggatctggtc ttggaggatt
     5461 agttaaagaa attgaaattg taaaagaatt aaactattca gacattcccg gatttccagt
     5521 gtcaacagtt aaaggtcatg gtaacaaact tattttcgga acattaaacg gagtaaaggt
     5581 agtagcgatg caaggtcgct tccattttta tgagggatac tccatgcagg aagttgcaat
     5641 gcccgtgaga gtgatgaaat ttcttggaat aaaaaatctg attctttcca atgcaagtgg
     5701 tggtgttaat ccaaaatata aagtaggcga tttgatgatt ttggatgacc acataaatct
     5761 gatgggtacc aacccgttga taggacctaa caatgatgat ttaggcccac gctttccgga
     5821 tatgggcgag ccttacgata aaacaatgat taaaaatgca aaagccattg cagacaaaca
     5881 tggctttacc tgccatacag gaatttatgc tgccgtaaca gggccaacgt ttgaaacacg
     5941 tgcagaatac aaatacatta aatttatagg tgcagattgt gtgggcatgt caactgttcc
     6001 ggaaaacatt gttgctcgtc acatgaatct gcctgttttt gctatctcta taatcaccga
     6061 cttaggcgga agcgatgaac cggagtttat tactcacgaa gaagttttaa aagttgctaa
     6121 tgcagcagaa cagaaaatga ccgtaattat caaagaactt attgcacaat aatttttatt
     6181 tcaatttatt tcagaagaag aatcatgaga aaaagaattc aatttaatat ctctttattt
     6241 agttttatca tttgcttttt tatttgcaca ttgcaaaaag tacaagcaca attttggtta
     6301 cccattggct caggcgtaaa cgataatgtt tatgcaatga ccaaggatac cgttaacaac
     6361 attctttaca tcggtggaag atttacagat gccagtggcg ttactgccat acgcatagca
     6421 tcatggaatg gaacaaccta tacagctata ggaaatggat ttgacagtaa tgtatttgcg
     6481 ctatattatg atcatagcaa caatacactt tatgcggcag gctcgtttct tttttcagga
     6541 ataaat
//
LOCUS       DAOOOI010000104         6363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2358_length_6363_cov_13.633323, whole genome shotgun sequence.
ACCESSION   DAOOOI010000104 DAOOOI010000000
VERSION     DAOOOI010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2358_length_6363_cov_13.633323"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..2518
                     /locus_tag="PKX59_09815"
     CDS             254..2518
                     /locus_tag="PKX59_09815"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOZ91202.1"
                     /translation="MAFDIEMIKKVYAEMPARIEKARKKLNRPLTLTEKILYSHLSPQ
                     FEVKNYDRGKAYVDFNPDRVAMQDATAQMALLQFMQAGRPQVAVPSTVHCDHLIQAKD
                     GAAKDLASALDKSKEVFDFLASVSNKYGIGFWKPGAGIIHQVILENYAFPGGMMIGTD
                     SHTVNAGGLGMIAIGVGGADACDVMAGLPWELKMPKLIGVKLTGKLSGWTSAKDVILK
                     VAGILTVKGGTDKIVEYFGEGAESMSCTGKGTICNMGAEIGATTSIFNYDKKMADYLR
                     GTGRADVADLADGIAAHLRGDTEVYQQPEKYFDEVIEINLSELEPHINGPFTPDLAWP
                     LSKFAAAVKENGWPAKLEVGLIGSCTNSSYEDITRAASLAKQAAEKGIKAKSEFTITP
                     GSEQVRYTVERDGYLGIFEKIGGVVLANACGPCIGQWARHTNDPNKKNSIITSFNRNF
                     AKRNDGNPNTHAFVASPEIVTALAIAGDLTFNPVTDFITNDKGEKVKLAEPQGIELPI
                     KGFAVEDAGFQAPAKDGSKVEVIVSPTSDRLQLLAPFAAWEKSDLKGLKLLIKAKGKC
                     TTDHISMAGPWLKYRGHLDNISNNMLIGAVNAFNDNTNSVKNQLDGSYDEVPKVQRAY
                     KAKGIGSIVVGDENYGEGSSREHAAMEPRFLGVRAVLVKSFARIHETNLKKQGMLALT
                     FADKADYDKIKEDDTFDIIGLTSFTPGVPLTLVLHHADGSKEEIKVNHSYNEHQIAWF
                     KAGGALNIIRSQQA"
     gene            2624..2932
                     /locus_tag="PKX59_09820"
     CDS             2624..2932
                     /locus_tag="PKX59_09820"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HOZ91203.1"
                     /translation="MAKKFSVKTNLHRRDIRGVSTYVVLREASDHFNETGVIEVIGFI
                     DNHPIEGALQPKTDGSHWLTIKKEWREAIGKTIGDSVEISIEIRKKISPPQKAKKKLF
                     "
     gene            complement(2924..4639)
                     /gene="recJ"
                     /locus_tag="PKX59_09825"
     CDS             complement(2924..4639)
                     /gene="recJ"
                     /locus_tag="PKX59_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOZ91204.1"
                     /translation="MNKRWTLLKQADNDKSIKFGKELNINPVLANLLIQRSVNTYDEA
                     KEFFRPSLSLLHDPFLMTDMDKAIARIDKAIAKGEKVLIYGDYDVDGTTAVALVYRFF
                     KNRFAECNFYIPDRYAEGYGISFQGIDWAKENGFTLIIALDCGIKAMDKTKYASDMGI
                     DFIICDHHRPGAEIPQAAAVLDPKRVDCAYPCKDLSGCGVGFKLLQAFAKKNNIEEQQ
                     VLDLIDLVAISIAADIVPILGENRILAYYGLELINKNPRTGIKAILELNAIRRELNIT
                     DVVFIIAPRINAAGRMDHGRSAVQLLISDSVGDAITESEKINKNNIDRRDADKLITEH
                     ALQQISRDNTFENRRSTVVYSAEWNKGVIGIVASRLTEKFYRPTVVLTDSNGHIAGSA
                     RSVKDFDVYNAIEACSEYLDQFGGHMYAAGLTLKHENFEAFKQKFEEVVCATIEEHML
                     TPEIEIDAELKLESITKPFYNVLKQFAPFGPGNMSPVFVSRNCIADKNQTRPVGQENK
                     HLKLAFTQDGFGKNFSAIGFQLGEYADLLKSGKRFDVCYHIEENHFNGNTELQLNIKD
                     IKISE"
     gene            complement(4742..6127)
                     /locus_tag="PKX59_09830"
     CDS             complement(4742..6127)
                     /locus_tag="PKX59_09830"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOZ91205.1"
                     /translation="MQKQKYFFTVLFWVLGLSLHAQTGTETPYSRYGIGELLNSGFAR
                     QSAMGGLGAAFQSSSAVNFSNPASYSSIRISTFETALRGELAQTSSSDLKQTKKGILL
                     NYLSLGFPVMKDNWGASFGLIPVSSTGYNLKEPGQVVNPNDTITTDFTKYYEGKGGLS
                     RFYIGNGFAPYSKTLDKYYASQHYKDLKEKGDTATINKEVRWRKIVKGFSAGFNASYL
                     FGSLNNIRRVKFSNSAFINTKITESTAYADFYFDYGIQYRYDFNEDNKLIIGISGAGG
                     TNIKGRRDVLWTLVSSSDFEANIDTVSLSENEKGKAFIPSFINAGFMFQQGEKWLMGA
                     EYATQQWSRYKAFNSNQNLKDSWRISAGAQYMPTNKLAYRAGIKVEQSYINLNDSRVN
                     DYSLSLGVGVPVRLTSTERRLFDNRTMLHFSIEGGQLGTTSNNLVKQQYVRLYVGITA
                     NELWFIKRKYD"
     gene            complement(6140..>6363)
                     /locus_tag="PKX59_09835"
     CDS             complement(6140..>6363)
                     /locus_tag="PKX59_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="pantothenate kinase"
                     /protein_id="HOZ91206.1"
                     /translation="TKTSIASGVQHGIVAEAEHLIGIYKRQFKGLKVIVTGGDMAFFA
                     DRLKTHIFAAPDLTLVGLNSILNYNLSRQ"
BASE COUNT         1959 a         1245 c         1268 g         1891 t
ORIGIN      
        1 cgaataagct gactaaataa ttaatttgtt tttgatttga tttcatgatt ttgatttttt
       61 aagattttgt tttcgattca aatgtataat gtttaaataa gaaaaacaag ttgccataaa
      121 aatttgttaa gtgtttcaag aaatgaaatg aatgcattta caatgtttga aaatccaata
      181 tttaggttga aaaaactatt gaatcaccat attacttatt tttgtaacca atacacataa
      241 aacattaaca cacatggctt ttgatattga aatgattaaa aaggtttatg ctgagatgcc
      301 tgccagaatt gaaaaggctc gtaaaaagct caaccgccca ctcacactta cagaaaagat
      361 tctctatagc cacctatcgc cacagtttga agtaaaaaat tacgatcgcg gcaaggctta
      421 tgtagatttt aatccggata gagtagccat gcaggatgct acggcacaaa tggctttgtt
      481 acaatttatg caagccggca gaccacaggt tgcagtgcct tcaacagtac attgcgacca
      541 cttgattcag gcaaaagatg gcgctgcaaa agatttagct tctgccttag ataaaagtaa
      601 ggaagtgttt gacttcttgg catcggtctc aaataaatat ggaataggtt tttggaaacc
      661 gggtgcagga attatccatc aggtaatttt agaaaactat gctttccccg gaggaatgat
      721 gattggaact gacagccata cagtgaatgc cggaggattg ggaatgattg ccattggtgt
      781 tggcggtgcc gatgcctgtg atgttatggc aggactacca tgggaactga aaatgccaaa
      841 attgataggt gtaaaactta caggtaagtt aagcggatgg acctctgcca aagatgtgat
      901 attaaaagta gcaggaatat taaccgttaa aggcggaaca gataagatag ttgaatattt
      961 tggtgaaggt gcagagagca tgtcatgtac aggcaaagga acaatctgta acatgggagc
     1021 agagatagga gctacaactt ctatttttaa ttacgataaa aaaatggctg actacctgcg
     1081 cggaacagga cgtgccgatg tagctgacct tgccgatggc attgctgccc acctgcgtgg
     1141 cgacacagaa gtatatcagc aacccgaaaa atattttgac gaagtgatag agattaacct
     1201 ctctgaatta gaacctcaca tcaatggtcc ttttactccg gatttagcat ggccgttgag
     1261 taagtttgct gcagcagtga aagaaaacgg gtggcctgca aaattagaag tcggactgat
     1321 tggctcttgt accaactcat cttacgaaga cattacacgt gcagcttcat tagcaaaaca
     1381 agcagcagaa aaaggaatca aagctaaaag tgaatttaca attacacccg gcagtgagca
     1441 ggttcgttat actgttgaac gcgatggcta cctcggtatt ttcgaaaaaa taggaggtgt
     1501 ggttttagca aatgcctgtg gtccttgcat tggtcagtgg gctcgccata ccaacgatcc
     1561 aaacaaaaag aattctatca tcacatcttt caaccgcaac tttgcaaaga gaaatgatgg
     1621 caacccaaat acacatgcct ttgttgcttc gccagagata gtaactgcat tagccattgc
     1681 aggcgacctt acttttaatc cggttacaga ttttatcaca aacgataaag gagaaaaagt
     1741 taagttagcc gaaccgcaag gaatagaact tcctattaaa ggttttgctg ttgaagatgc
     1801 aggctttcag gctccggcaa aagatggcag taaagttgaa gtgattgtgt cgccaacaag
     1861 cgaccgctta cagttgctag caccatttgc tgcttgggaa aagtcagacc ttaaaggctt
     1921 gaaacttctt atcaaagcaa aaggcaaatg tactaccgat catatttcaa tggcgggtcc
     1981 gtggttgaaa tacagaggac atctcgataa catttccaac aacatgctta ttggtgcagt
     2041 aaatgcattt aatgataaca ccaacagtgt aaagaatcaa cttgacggca gctatgatga
     2101 agtgcctaaa gtgcaacgtg cctataaggc aaaaggaata ggctctattg ttgtgggtga
     2161 tgaaaactat ggagaaggct catcgcgtga acatgcagct atggaaccgc gtttcttagg
     2221 cgtacgtgca gtgttggtaa aatcgtttgc ccgcattcac gaaaccaatc ttaagaaaca
     2281 aggaatgctt gcactgacgt ttgccgataa agcagattat gataaaatta aggaagatga
     2341 tacttttgat attatcggtt tgacatcatt cacacccggt gttccattaa cattggtatt
     2401 gcatcatgct gatggctcaa aagaagaaat taaagtaaat cattcgtata acgaacatca
     2461 gattgcctgg ttcaaagcag gtggtgcact aaacatcatt cgcagccaac aagcctgatt
     2521 ttaaaattca gtcaaataga aaactccgga ataaaaaatt tattccggag tttttcattt
     2581 ttattttctt atctttatag ggtaaaataa aaacagcatt gctatggcga aaaagtttag
     2641 tgtaaaaaca aatctccatc gcagagacat cagaggtgtt tctacctatg tggtgttgag
     2701 agaagcaagt gaccacttta acgaaacggg tgttattgaa gtaataggat ttatagacaa
     2761 tcatcctatt gaaggtgcct tgcaaccaaa aacagatggc tcacattggc tgacaataaa
     2821 aaaagaatgg cgcgaagcta ttggcaaaac cattggtgat agtgttgaaa tttctattga
     2881 gataagaaag aaaatatcac ctccacaaaa agcaaagaaa aaactattct gaaattttta
     2941 tatccttaat gttgagttgc aactctgtgt taccattaaa atgattctct tcaatatggt
     3001 aacaaacatc aaagcgtttt cctgatttga gtaaatcggc atattcgcct aactgaaacc
     3061 ctatggcaga aaaattcttt ccaaatccat cttgtgtgaa agcaagtttc agatgtttgt
     3121 tttcctgacc aacaggtcgt gtctgatttt tgtcggcaat acaattgcgg ctgacaaaca
     3181 caggcgacat attaccggga ccaaatggtg caaattgttt cagcacatta tagaatggtt
     3241 ttgttatact ttctaacttt agctctgcat caatttcaat ctccggtgta agcatgtgct
     3301 cttcaatggt agcacaaact acttcttcaa acttttgttt aaaggcttca aagttttcat
     3361 gctttaatgt gagtccggct gcatacatgt gtccgccaaa ctgatctaaa tattcactgc
     3421 atgcttcaat agcattatac acatcaaaat cttttacaga gcgtgcagag cctgcaatat
     3481 gcccgttgct gtcggtaagc actaccgtag gacgataaaa tttttctgta agtcgtgaag
     3541 ctacaatgcc tatcacacct ttattccact ctgcactgta aacaacggta cttcttcggt
     3601 tttcaaaagt attatcgcgg ctaatctgct gcaaggcatg ttctgttatg agtttgtcgg
     3661 catcgcgcct gtcaatgttg tttttattga ttttttcgct ctcagttata gcatcgccaa
     3721 cagaatcgct gatgagcaat tgcactgccg accttccatg atccattctt cctgctgcat
     3781 taatgcgtgg tgcaataata aacaccacat cggtaatgtt gagttcgcgc ctgatagcat
     3841 ttaactcaag aatagctttt attcctgtac gtggattttt attgataagc tcaagaccat
     3901 aataggctaa gattctgttt tcgccaagaa taggaactat atctgcagca atacttatgg
     3961 caacaaggtc aataaggtcg agcacttgtt gctcttcaat attatttttt ttggcaaatg
     4021 cctgaagcag tttaaagcca acaccacagc ctgaaaggtc tttgcaggga taggcacaat
     4081 ctactcgttt tgggtcgaga acagcagcag cttgtggaat ttctgctccc ggtctgtgat
     4141 gatcgcagat gataaaatct atacccatgt ctgaggcata ctttgtttta tccatagctt
     4201 ttatcccgca gtcaagtgct atgattaacg taaatccgtt ttcttttgcc caatctattc
     4261 cctgaaaaga aattccatat ccttcagcat atctgtcggg aatataaaag ttgcactcgg
     4321 caaagcgatt tttaaaaaaa cgataaacca acgctactgc cgtggtgcca tcaacatcat
     4381 aatcaccata aatcaatacc ttttcacctt tagcaattgc tttatctata cgggcaatag
     4441 ctttatccat atctgtcatc agaaatggat cgtgaagcaa agacaatgaa ggtctgaaaa
     4501 actcttttgc ctcgtcatag gtgtttacac tgcgctgaat caacaagttg gcaagcacag
     4561 ggttaatatt taactcttta ccaaacttta ttgacttatc attatctgcc tgtttcagca
     4621 gtgtccagcg cttgttcatt ttgttttctg tcagcaaata taagcaccct cactatagct
     4681 atagagtgtg agctgagcta aaacttaaaa aagctgatta atgcactgat ttaacgctcc
     4741 gttaatcgta cttccgtttg ataaaccaaa gctcgttggc tgtgatacct acataaagcc
     4801 tcacatattg ttgtttaact aggttgttgc ttgtagtacc caactgtccg ccttcaatgc
     4861 taaaatgaag catggtgcgg ttatcaaaca atcttctttc tgttgaagtc agacgtacag
     4921 gcacacccac acctaaagaa agtgagtagt cattaacacg gctgtcattg aggttgatgt
     4981 acgattgctc aacttttatt ccggcacggt aggctaattt atttgtaggc atgtattgtg
     5041 cgcctgcaga aatacgccat gagtctttaa gattttgatt ggagttgaaa gctttgtatc
     5101 tgctccattg ctgtgttgca tattcagcac ccataagcca cttttcacct tgctgaaaca
     5161 taaatcctgc attgataaaa gaaggaataa atgctttgcc tttttcgttt tcagataaag
     5221 aaacagtgtc aatatttgct tcgaaatctg atgaggaaac caatgtccaa agcacatcac
     5281 gtctgccttt gatgtttgtt cctcctgcac ctgaaatacc tatgataagt ttattgtctt
     5341 cattgaaatc atagcgatat tgaatgccgt aatcaaaata aaaatcggca taggcagtgc
     5401 tttctgtaat ttttgtattg ataaatgctg aattgctaaa ctttaccctg cgaatgttat
     5461 tcaggcttcc aaaaagatag gatgcattaa aacctgcaga aaaacctttt acaatttttc
     5521 tccatcgcac ttctttatta atcgttgctg tgtcgccttt ttcttttaag tctttataat
     5581 gttgcgatgc ataatattta tcgagcgttt ttgaataggg tgcaaaaccg ttacctatgt
     5641 aaaaacgact caatccgccc ttcccttcat agtatttggt gaaatctgtg gtaatggtat
     5701 cgttagggtt aaccacctgg ccgggttctt tgaggttgta gcctgtagag cttacaggaa
     5761 ttaatccgaa gctggcaccc cagttgtctt tcatcaccgg aaaacctaaa gatagatagt
     5821 tgagcaaaat tcctttttta gtctgtttta aatctgatga tgatgtttgg gcaagctcgc
     5881 cacgcaatgc agtttcaaaa gtgcttatgc gaatagatga atacgaagcc ggattgctga
     5941 agttaacggc agatgaactc tgaaaggctg ctcccagtcc gcccattgcc gactgacgtg
     6001 caaaaccact gttaagcaat tcgccaatac catatcgcga atagggtgtt tctgttcccg
     6061 tttgtgcatg caatgaaaga cctaaaaccc aaaaaagtac tgtaaaaaaa tatttttgtt
     6121 tctgcattat caacttgttt tactgccggc ttagattgta atttaaaata ctgtttaacc
     6181 caacaagggt taaatccggg gctgcaaaga tgtgtgtttt cagcctatct gcaaaaaacg
     6241 ccatatcgcc acctgtaaca atcactttaa gacctttaaa ctgtctttta taaataccta
     6301 tcaaatgttc tgcctctgca actatgccat gctgcacacc actggcaata gaggttttgg
     6361 tag
//
LOCUS       DAOOOI010000105         6332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2373_length_6332_cov_6.018639, whole genome shotgun sequence.
ACCESSION   DAOOOI010000105 DAOOOI010000000
VERSION     DAOOOI010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2373_length_6332_cov_6.018639"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PKX59_09840"
     CDS             complement(<1..606)
                     /locus_tag="PKX59_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91207.1"
                     /translation="MQKKSQNEKKSYEISLSLFLLFLFFTIISKAQDVEHINLKKPVT
                     FHGNLNFQLDYYSANGIPARQKEFSWIINGNPVLNVLGIDLPFTFLFSNFGNKYSQPF
                     NQFGLSPRYKWITMHLGYRNLTYSNYTLAGHKMLGVGFDLNPKKFRIGFMYGQLRRSA
                     SIDSTMNTNPMYVRSTPTFKRVGIAGKIGYGSQKNYVDLVYF"
     gene            complement(876..1277)
                     /locus_tag="PKX59_09845"
     CDS             complement(876..1277)
                     /locus_tag="PKX59_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91208.1"
                     /translation="MLKDIPQLKVENVAVAIVKEQSNGADVWNAYIINLKNEMLEGVL
                     ITSKGYGMMEGEERKTSTLRHFIENIPPQSCVKIEPITDNVLNLNNEYWVSFYLNNTM
                     YDRKYVFLPGVINEEFFTQIPLLEKRGVMIK"
     gene            complement(1324..2457)
                     /locus_tag="PKX59_09850"
     CDS             complement(1324..2457)
                     /locus_tag="PKX59_09850"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOZ91209.1"
                     /translation="MKKIIKYLLFILLITTVVLAATGKLYYYKALVYNFVDIDDLNLF
                     HNREVKNGTGMPWNVSTDYNKKNLSDTLTALLEANKSVAFLVIKNDSICFEKYWEDYG
                     QQSLSNSFSMAKSIVALLIGKAIDEGKIKSLDQPVADFIPEFKEGEKSKITIRNLMMM
                     SSGLDWNEGYSSLTSQVTEAYYGTQLYKQVASLGVAEAPGKFWEYKSCDTELLAIIVT
                     NATGKSLAEYASEKLWKPLGAEHSAQWSLDHKDGIEKAYCCFYSNARDFARFGKLVLQ
                     HGRWNNEQIIDSAFITLCTTPNNLPDKEDNKPVQHYGYQWWLSNISNHPVYYMRGILG
                     QYVIVIPDMQMIIVRLGHKRSNNPDGKPNDYKFYAEQVIKMYE"
     gene            complement(2454..3191)
                     /gene="lipB"
                     /locus_tag="PKX59_09855"
     CDS             complement(2454..3191)
                     /gene="lipB"
                     /locus_tag="PKX59_09855"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009391266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOZ91210.1"
                     /translation="MQEILFADLGASDYKQTWDFQEQLLQQIMQLKLEGRNHGFGDHA
                     PTPHYLLFCEHPHVFTLGKSGKPEHLLSNEQQLKTANATYYKINRGGDITYHGPGQIV
                     GYPILNLDLFFNDIHRYMRFLEEVIIRTLKEYDIEGSRIEKLTGVWIDAGKPSARKIC
                     AFGVRCSRWITMHGWALNVNTQLDYFNHIVPCGISDKAVTSMAKELGKNLNIDEVKSV
                     LLKHFSEVFNATIKNVGKEKLIAKEKT"
     gene            complement(3257..>3804)
                     /locus_tag="PKX59_09860"
     CDS             complement(3257..>3804)
                     /locus_tag="PKX59_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOZ91211.1"
                     /translation="HYDHLGGMGNKTFFPGANDNASGVAMLLSLAEYFSKKENQQPYS
                     ILFIAFAGEEAGLQGSKYYTEHPILPLSKIRFLINLDLLGTGDDGIMVVNATEFEKDF
                     SLLQSINDIAKFVSNIGKRGKAKNSDHYYFSEKGVPAFFIYTLGGVTWYHDVMDKAET
                     LPLTKYNNVFQLLKSFVLALQ"
     assembly_gap    3805..3814
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3815..4519)
                     /locus_tag="PKX59_09865"
     CDS             complement(<3815..4519)
                     /locus_tag="PKX59_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91212.1"
                     /translation="MLRIPSLASLIFICAFLQTHFVFAQQDDARKVITELTSEKYFGR
                     GYLKHGDKKAAHFIKKQFSKLGLKPVDDKHWFQHFTHPVNRFPKNVSLTTDTKNWIPG
                     KEFLIHHQSPSANGTWPIAWADSAEIISRLNNDYDSCYVITRQVFSKQQKEITALLFK
                     RKKGALVILEPHKLTWSVGTSVFNIPVVEVYDSLFSRQTKNITIKADAVFDAGYDSKN
                     VIGLITGNKTPDSLIVI"
     gene            complement(4519..5316)
                     /locus_tag="PKX59_09870"
     CDS             complement(4519..5316)
                     /locus_tag="PKX59_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91213.1"
                     /translation="MTKQSITSEIQVAIEQIYRQWGVFSLDNRIPENEELVMLNSKLM
                     KLHDKINHLQLLESVYAYKQPIQEVITETVQKPITQTVIEKSETEQKITIEAPEVKTF
                     EPIIAEVTEPTPIKPEVHTATTTAEEVKQKKVTASVVPPVQTARAEVFKEADQTVATM
                     FHETETIHDKIGVAQPAFAIADKLKLSPVTDLVKAIGINEKFLFISQLFNDDSIKYQS
                     AIDKLNTSSNFAEAYMYFDSEITSGNQIDKNSDACRQFVDLLQRRFM"
     gene            5470..>6332
                     /locus_tag="PKX59_09875"
     CDS             5470..>6332
                     /locus_tag="PKX59_09875"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOZ91214.1"
                     /translation="MLQVATFTFAAVKKPILLFTFFLLSVTLFAADQVLFIKDKSNGK
                     PVNEAVVVWIMPSGKSQILLPDNNGRVKLPKTEEKNFSIEVRCLGYQSQKIEKENSGD
                     TIWLSRNEVSLNEVAVTASYAPAPAMQSAYAIKIISKESFDNMAAVNVADVLSKQLNM
                     STGYDQAFGSTLTMMGMDMKNIKVLIDGVPVIGRMNGNIDLNQLNLAAVERIEVVEGP
                     MSTAYGTDAIAGAINLITKPQSLKKTTAGASAYYENIGTYNTDLNVATNFKHAGIKIT
                     AGRNFFEGWNLT"
BASE COUNT         1928 a         1143 c         1086 g         2165 t
ORIGIN      
        1 aaaataaacc agatcaacat aattcttttg tgagccataa ccgattttac ccgctatgcc
       61 aacacgttta aaagtaggtg ttgaacgcac gtacattggg tttgtgttca tagtggaatc
      121 aatagatgct gatcgcctta gttgtccata cataaatcct attcggaact tttttggatt
      181 caaatcaaat cctacaccta acattttgtg gccggccaac gtatagtttg aatatgtgag
      241 gttacgatag cctaaatgca tagtaatcca tttgtatcgt ggactcaatc caaattgatt
      301 aaatggctgc gaatatttat tgccaaaatt tgaaaaaaga aaagtaaatg gcaggtcaat
      361 tcccaataca tttaaaacag gattgccatt gataatccaa gaaaattctt tttgccttgc
      421 aggaatacca ttggcactgt aataatccaa ttgaaaatta agatttccat gaaatgttac
      481 aggctttttc agattaatgt gttcaacatc ctgcgcctta ctaattatgg taaagaataa
      541 aaaaagtaaa aatagagata atgaaatctc ataacttttt ttttcatttt gtgatttttt
      601 ctgcatgcgg atatattgaa aaaaaatcaa tcgtaaaaat cactcaatta agttgtttaa
      661 ttctgatata ccattaatca catataaaca actcaatcaa tcacaaatta ctttcgtgtt
      721 agggtattta gaaagtaata agcaaatgta attgttttaa gaaattaaaa aggaaatttt
      781 ctaaatattt ttaactgagt tataattaca tcctgttaaa attcctatat tgattcgaag
      841 acttataaag gggtctataa taaatttctt ctaaatcact taatcatcac cccgcgtttt
      901 tcgagtaagg gtatctgtgt aaaaaattct tcatttataa ctcccggaag aaaaacatac
      961 tttcgatcgt acatggtgtt gttgaggtaa aagcttaccc agtattcatt attcaggtta
     1021 agaacattat ctgtaatagg ttcaatcttt acgcacgact gcggaggaat attttctata
     1081 aaatgtctaa gcgttgatgt ttttctttct tcaccttcca tcatgccata gcctttgctt
     1141 gtaatcaaca caccttccag catttcattt ttcaggttaa tgatataggc attccaaaca
     1201 tcggcaccgt ttgattgctc ttttacaatt gcaacggcta cgttttcaac ttttaattga
     1261 ggaatatctt ttagcatatt atattaaacg agtaatgata atagaagtta ccttgatagc
     1321 ttgttattca tacattttta tgacttgttc ggcataaaat ttatagtcat taggctttcc
     1381 gtccggattg ttactgcgtt tatggcctaa acgcacaata atcatctgca tatcaggaat
     1441 aacaataaca tactgcccta agatgccacg catgtaatac acaggatgat tgctgatgtt
     1501 tgacaaccac cactgatagc cgtagtgctg aacaggttta ttatcttctt tatccggtaa
     1561 attatttggt gtggtacaga gagtaataaa agctgaatca ataatttgtt cattattcca
     1621 tctgccatgt tgcaaaacaa gtttaccgaa acgtgcaaag tcgcgagcat tgctgtaaaa
     1681 acaacaataa gctttttcta ttccgtcttt atgatcgagg ctccattgtg cactatgttc
     1741 tgctcccaat ggtttccaaa gtttttctga tgcatattct gccagacttt ttcctgttgc
     1801 attagtcacg atgatagcta acaattctgt gtcgcagctt ttatattccc agaattttcc
     1861 cggtgcttct gcaacaccta atgaggcaac ctgtttgtaa agttgtgttc cataataagc
     1921 ctccgtaacc tgtgatgtca gcgatgaata tccttcattc cagtcaagtc ctgaactcat
     1981 catcatcaga tttcgaattg taatttttga tttttcaccc tctttaaatt caggaataaa
     2041 gtctgcaaca ggctgatcaa ggcttttaat ttttccctcg tcaattgctt ttccaatcag
     2101 taaagcaact atactctttg ccatagagaa tgaattggaa agcgattgtt gcccataatc
     2161 ttcccaatat ttttcaaaac aaatagaatc gttttttatg actaaaaatg caacagattt
     2221 attggcttcg agcaatgcgg ttagtgtatc actcagattt tttttattgt agtctgtaga
     2281 aacattccat ggcattcctg ttccattttt tacttctctg ttgtgaaaca aattcaaatc
     2341 gtcaatgtca acaaagttgt aaacaagagc tttatagtaa tacaactttc ctgtagcggc
     2401 aagtactact gttgttatca ataaaataaa cagaaggtat ttgattatct ttttcatgtt
     2461 ttttcttttg ctattagttt ttcctttcct acatttttaa ttgtggcatt aaaaacttca
     2521 ctgaaatgtt ttagtaagac agattttact tcatcaatat ttaaattttt tccaagctct
     2581 tttgccattg atgtaactgc tttatcgctg atgccgcagg ggacaatgtg gttaaaataa
     2641 tctaactgtg tattaacatt aagtgcccat ccatgcatag ttatccatct gctgcaacgc
     2701 acaccaaaag cacaaatttt tcttgcacta ggtttgcccg catcaatcca tactcctgta
     2761 agtttttcta ttctgcttcc ctcaatgtca tattccttca aagtacggat aattacttct
     2821 tcgagaaaac gcatgtagcg atgaatgtca ttaaaaaata agtcaagatt cagaataggg
     2881 taaccaacaa tctgtcccgg gccatgatag gtaatatcgc caccacgatt tattttgtag
     2941 taggtggcat tggcagtttt aagttgttgt tcgttagaga gcaaatgttc gggctttccg
     3001 ctttttccta aggtgaaaac atgagggtgt tcacaaaaca atagataatg tggtgttggt
     3061 gcatgatccc caaaaccgtg attacgccct tctaatttca gttgcataat ctgttgaaga
     3121 agctgctcct gaaaatccca ggtttgttta taatccgatg cgcctaaatc agcaaaaagt
     3181 atttcctgca cttttgtttt tataatttac aaagataaat gaatagaaga aacgattttt
     3241 cagtgataaa gtttgcttac tgcaaagcta agacgaatga tttcaaaagc tgaaatacat
     3301 tgttgtactt tgttaaaggc aatgtttctg ctttatccat cacatcatga taccatgtaa
     3361 caccaccgag ggtatagata aaaaatgctg gtacaccctt ttcactgaaa taatagtgat
     3421 cgctgttttt tgctttacct cttttaccaa tattgctaac aaattttgct atatcgttga
     3481 tagactgcaa caacgagaaa tctttttcaa actcagttgc attgacaacc ataatgccat
     3541 catcgcctgt gccaagcaaa tcgagattta tcagaaaacg gatttttgat agtggtaata
     3601 taggatgttc tgtataatat tttgacccct gaagtcctgc ttcctctccc gcaaaagcaa
     3661 taaataaaat ggagtagggc tgttggtttt ctttttttga aaaatattct gctaaagaaa
     3721 gtaacatggc aacaccgctt gcattgtcat ttgcccctgg aaaaaatgtt ttatttccca
     3781 taccccctaa atgatcataa tgtgnnnnnn nnnntatcac aattaaagaa tcaggtgttt
     3841 tatttccagt aattaatcca attacatttt ttgaatcata gcccgcatca aatacggcat
     3901 cggcctttat ggtaatattt tttgtctgac gactgaacag actatcatat acttcaacaa
     3961 caggaatatt aaaaactgat gtaccaacac tccatgttag tttatgcggt tcaagaatta
     4021 ccaatgctcc tttttttcgt ttaaacagta aggctgtaat ttctttttgc tgcttactga
     4081 aaacctgacg tgttatgaca taacaactgt cataatcatt gttaagtctt gaaataattt
     4141 ctgcagaatc tgcccaggct ataggccatg ttccattggc agatggcgat tgatgatgta
     4201 ttaaaaactc tttcccggga atccaatttt ttgtgtctgt agttagactt acattttttg
     4261 gaaaacggtt tacaggatgt gtaaaatgct gaaaccaatg tttatcatca acaggtttta
     4321 atccaagttt gctgaattgt ttttttatga aatgtgcagc tttcttatca ccatgtttta
     4381 aatagccacg accaaaatat ttttctgaag ttaattcagt aataactttt cgcgcatcat
     4441 cttgctgtgc aaaaacaaag tgtgtctgta gaaatgcaca aataaaaatc agcgatgcaa
     4501 gagaaggtat tcgcagcatt acataaatcg cctttgtagc aaatctacaa actgtctgca
     4561 ggcatcactg tttttgtcta tttgatttcc tgaagttatt tcagagtcga agtacatgta
     4621 agcttctgca aaatttgagg aagtattcaa cttatcaatt gctgattgat attttataga
     4681 atcatcatta aacaactgac tgataaataa aaatttttcg tttatgccta ttgctttaac
     4741 caagtccgta actggtgata gttttaactt atctgctatg gcaaaagccg gttgcgccac
     4801 accaatttta tcgtgtatgg tttctgtttc gtgaaacatg gttgctacag tttgatctgc
     4861 ttctttaaat acttcagctc ttgcagtttg tacaggagga accacagaag ctgtaacttt
     4921 cttctgtttc acttcttctg ccgttgttgt agctgtatga acttccggtt taatgggagt
     4981 aggctcagtg acttcagcta ttatcggttc aaaggttttt acctccggtg cttcaatggt
     5041 tattttttgt tccgtctcag atttttcaat gacggtttgt gttattggtt tttgcactgt
     5101 ttctgtaatt acttcctgaa taggctgctt ataagcataa acagattcaa gtaattgaag
     5161 atgattgatt ttatcgtgca gcttcatcag tttgctattc agcatgacta attcttcatt
     5221 ttcaggaatg cggttgtcta atgaaaaaac gccccattgc ctgtaaattt gttctattgc
     5281 aacttgaatt tcgctggtga tagattgctt tgtcatgaaa ttgtgttttt atgctaacgc
     5341 taaataaatt aattcatttt tgatttggaa aatttatggg taatctcaga aaattttcat
     5401 gagcaaaatt aattgtcata ctactgtcaa cattgttggt tgactgatat ttatcaacat
     5461 aacttttcca tgctgcaagt cgctactttt acttttgcag ctgtgaaaaa acccatactc
     5521 ctattcacat tttttctatt gtctgtaacg ctttttgctg ccgatcaggt attatttatc
     5581 aaagacaaat ccaatggcaa gccggtaaat gaggctgttg tggtttggat aatgccttcc
     5641 ggaaagtcac agattttatt acccgacaac aatggtaggg ttaaattacc taaaacagaa
     5701 gagaagaatt tttcaattga agtgcgttgt cttggttatc agagtcaaaa aattgaaaag
     5761 gaaaattccg gtgacaccat ttggctatcc agaaatgagg tcagcctcaa tgaggttgcc
     5821 gtaactgcat cttatgctcc tgctcctgcc atgcagtctg cctatgctat aaaaattatc
     5881 agcaaagaat cgtttgacaa tatggctgca gttaatgttg ccgatgttct cagcaaacaa
     5941 ctcaacatga gcacaggtta tgatcaggct ttcggcagta cattaaccat gatgggcatg
     6001 gatatgaaaa atattaaagt gttgattgat ggtgttcctg ttattggaag aatgaatgga
     6061 aatatagacc tgaatcagtt aaaccttgct gctgttgaaa gaatagaagt ggtagaaggg
     6121 ccaatgagta ctgcctatgg taccgatgcc attgcaggcg ctataaatct gataaccaaa
     6181 ccgcagtcgc ttaaaaaaac tactgcaggt gcttctgcat attatgaaaa tattggaacc
     6241 tacaataccg atttgaatgt tgcaactaac tttaaacatg caggaataaa aataactgcc
     6301 ggaagaaact tttttgaagg atggaattta ac
//
LOCUS       DAOOOI010000106         6289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2388_length_6289_cov_26.082130, whole genome shotgun sequence.
ACCESSION   DAOOOI010000106 DAOOOI010000000
VERSION     DAOOOI010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2388_length_6289_cov_26.082130"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PKX59_09880"
     CDS             <1..273
                     /locus_tag="PKX59_09880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91215.1"
                     /translation="KFQDFTGIRNVSSVNDFDSYPNPVTDKINFKTENIPFTIEVMDA
                     KGAVVYSEIINSAMGNIDVSHLKNGIYSFKAVSKNNVAVIKRFCKQ"
     gene            complement(276..3953)
                     /locus_tag="PKX59_09885"
     CDS             complement(276..3953)
                     /locus_tag="PKX59_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ91216.1"
                     /translation="MVFSAIVLEWNMHKRDLDFIRITVFAKKTLHEEENRANKYVEWL
                     GNMLSRQTSYSAIDDSLLLTSQKPVSAFYSFVYLNDSLIYWDNNAVSFSYNQVKNGIN
                     QQLIQLKNGWYELFRYQRRNITIIGLLPIRAGYDFQNKYLRNEFNPVLGIPEHALLLP
                     SPATGSYPVISSDGNYLFSIKYNPIMSDPGRLWYIAALYLAGFFLLLISFYLYARQWL
                     LKNPLVSFVIISLLVFIKWLMLTFRFPEFLYGMPLFNPKFYATSYFLNSLGDFLLSAS
                     VFCCVILFVYNYLHLRRRKIKVIRQSPTLLSIVVIGNLLFTFLFSVFINYLISGLILN
                     SKISFNVNNVFELTFFSLIGFLIIGILLFSFYVSCEGTVKFAEYSGFSLPYNFLLFLI
                     TQGLFLVVLILFRNTDVFVNYGVATFFLTNSLILFISYIRFTSRQNFSFVRYMFVIAG
                     FSVYAAYTISSFNMVREKNNMRLLVSKVENREDLIAEYLFQEIDQKLKTDPVITSNII
                     QGRSPLLEEKIKKRITLNAFNQAYWTRYDIQIKAYDSAGVSLFANSDTLRNVTFYDSI
                     INTQTRPTYSHSFNYLSSNSGKIGYIGKITYPKNASGELAATIIIQLDSKFNQEEGGF
                     PDLLLSEKMPASRDLSKYAYAKYENGKLIFQSGKFNYFHSAATYDRMFGLVKSDQFVS
                     LGGFIHLFYNQDKNNLIVISYKTPQAIEQVTLFSYVFIFFSATFILIYMIWLFLANNF
                     RLHLDFRKRIQLSVVGLVVIAILLIGGGSIFYISRGYSEEQKVRIKEKLSTLMLAVES
                     EFNDKNLTEANLTEETTLNFSRISNTLSSDFNLYDKNGRLIYSTQPKIFDKEIISRLM
                     NRSAFDQLKNYQTNGFIHDEKIGLLEYTSAYETIVNKDNKVAAYINLPYFARQNELKK
                     EISGFLVTLINIYVLLFGIAIVITFIISNRLVKPLNLIQQKLARVKLDSSNELLEWEG
                     GDEIGSLVNEYNKMVNKLAKSADALAQSERETAWREMAKQVAHEIKNPLTPMKLSMQH
                     LARAWKEKNSNFENIFEKVSRTMIEQIDSLSRIATEFSNFAKMPKGNFEKIDLVKSIQ
                     SNIDLYSEVRNVDFVFNKSNIDDEAVVNGDWEQTLRAFGNLIKNAVQAIPENQQGLIT
                     IQLTKADKQFYISITDNGKGITEEAKQKIFTPNFTTKSGGTGLGLAMVKSIVENMQGK
                     ISFESEYGKGTTFKIELFEAD"
     gene            complement(4084..6177)
                     /locus_tag="PKX59_09890"
     CDS             complement(4084..6177)
                     /locus_tag="PKX59_09890"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOZ91217.1"
                     /translation="MSIASASFAENKDLKEQSANPLLQPWTGPYNGVPAFDKVKEEYF
                     IPAFEAAFAEKKAEIQKIASNKEAPNFKNTIEAMEKAGATLNRVRAIYDIWSSNLNTP
                     VMTAIQEKIEPQIAAFNDEVIQNKPLFLRIEKVCSDTVGKKYSVEQHQLLKRYYNNFV
                     LAGAKLNDADKAKVANINSRLATLFTQFSKNQLADENEKYIEIKNESDLAGLPDDIKS
                     AYAKNAKERGKSTWTVANTRSAVEPFLTFSNNRELRKQVWTMFINRGDNGDANDNNKI
                     IPEILKLRYERAKLMGFATFAHQKLSDKMAKTPEATMKLMESVWPAAIARVKEEVADM
                     QAIADKENAGIKIEPWDYRYYAEQVRKSKYDLDQNEVKQYLQLEKLREGMFFVAGELF
                     NLSFTQVHDVPVFHPDVTVYKVEDKTTHKLKGIWYFDPYARTGKRSGAWMTAYREQEK
                     LSGNVTTIVSNNCNYIKGSGNDPVLISWDDATTLFHEFGHALHGLCSDVTYPTISGTN
                     VARDYVEFPSQVLERWLPTSEVLQKYAINAKTGQPLPQSLLEKLNKSSKFNQGFETTE
                     YLSSALVDMKIHLAGDKVTDPDKFEKETLAALNMPSEIVMRHRLPQFGHIFSSDEYAA
                     GYYSYLWSDVISADATEAFTEAGSLYDKKTAEKLYRNVFSVGASIDESIGYRNFRGHD
                     PDVKALMRSRGFPEK"
BASE COUNT         2015 a         1139 c         1034 g         2101 t
ORIGIN      
        1 aaatttcagg actttacggg aatacgtaat gtttcttccg tcaatgattt tgattcatac
       61 cccaatcctg ttacagacaa aattaatttc aagactgaaa atataccatt tactattgaa
      121 gtaatggacg caaaaggtgc cgttgtttat tcagaaataa ttaactctgc aatgggaaat
      181 atagatgtct cacatctaaa gaatggcatt tattctttta aggccgtttc aaaaaataac
      241 gttgctgtta tcaaacgttt ttgtaagcaa taattttaat ctgcttcaaa tagctctatt
      301 ttaaaagtag ttccttttcc gtattcactt tcgaacgata tttttccctg catattttct
      361 acaatacttt ttaccatagc aagtcctaaa cctgtgcctc cacttttagt tgtaaagttt
      421 ggcgtaaata ttttttgctt tgcctcttcg gtaatgcctt taccgttgtc agtgatacta
      481 atataaaact gcttatccgc ttttgtaagt tgaattgtaa ttaatccttg ttggttttca
      541 ggaatagctt gtactgcatt ttttataaga tttccgaaag ctcttaatgt ttgttcccag
      601 tcaccattaa caacagcttc atcgtcaatg ttgcttttat taaaaacaaa atccacatta
      661 cgcacttcac tgtaaaggtc aatattactt tgaatagatt ttacaaggtc tattttttca
      721 aaattgcctt ttggcatttt tgcaaagttt gaaaactctg tagctatcct tgatagtgaa
      781 tcaatctgct caatcattgt tctggaaact ttttcaaaaa tgttttcgaa gttgctgttc
      841 ttctctttcc atgcacgtgc aagatgctgc atactcagct tcattggtgt gagtggattt
      901 ttaatttcat gggcaacctg ctttgccatt tctcgccagg cagtttctct ttccgactgc
      961 gctaatgcat ctgcactctt agccaacttg tttaccatct tattgtattc atttaccaaa
     1021 gagccaattt catctccacc ttcccattca agcagctcat tactactgtc taattttact
     1081 ctagctaatt tttgctgaat aaggtttaat ggctttacaa gacggtttga aataatgaat
     1141 gtaataacaa tggcgatacc aaaaagtagt acataaatgt tgattagtgt aaccaagaaa
     1201 ccactgattt cttttttcaa ttcattctgc cgtgcaaagt atgggaggtt aatataagct
     1261 gctactttat tgtctttatt cacaatagtt tcgtaggctg aagtatattc caataaaccg
     1321 attttttcat catgaataaa accattagtc tgataatttt taagttggtc gaaagcactc
     1381 ctgttcatca accttgaaat aatttcttta tcaaatatct ttggttgtgt ggaataaatt
     1441 aatcgtccgt ttttgtcata aagattaaaa tcgcttgaaa gtgtattcga aattcttgaa
     1501 aagtttaagg tagtttcttc tgttaagttt gcctctgtta agtttttatc attgaattct
     1561 gattcaacag caagcatcag tgttgaaagt ttttctttaa tcctgacttt ttgctcttcg
     1621 gaataaccac gcgaaatgta aaaaatagaa ccaccgccaa tcaacaatat agcaataaca
     1681 actaaaccaa ctactgaaag ctgaattcgt tttctgaaat caagatgcaa cctgaaattg
     1741 ttggctaaga aaagccaaat catgtaaatt aaaatgaatg ttgcagaaaa gaaaataaac
     1801 acataagaga ataaagtcac ctgttcaata gcctgaggtg ttttatatga aattacaatc
     1861 agattatttt tatcctgatt atagaataag tgaataaacc ctcctaatga tacaaactga
     1921 tcggatttta ccaaaccaaa catacggtcg tatgtagcag cagaatgaaa atagttgaac
     1981 ttgccacttt gaaaaatcag tttgccattc tcatattttg cataagcata ttttgaaaga
     2041 tcgcgcgatg caggcatttt ttctgaaaga agtaaatcgg gaaacccacc ttcttcctga
     2101 ttaaatttag agtcaagctg aataattatt gtagcagcaa gttcaccgct ggcatttttt
     2161 ggatatgtaa tttttccgat gtatccaatt ttacctgagt tagaactcag gtaattaaag
     2221 gaatggctat aggttggtcg tgtctgcgta ttgatgatgc tatcataaaa agttacattg
     2281 cgcaatgtgt cggaattggc aaacagagaa acacctgcag agtcataagc tttgatttga
     2341 atatcgtagc gtgtccaata tgcctgatta aatgcattta gagtgatacg cttttttatt
     2401 ttttcttcta acaagggcga ccttccctga ataatatttg aagtaattac aggatcagtc
     2461 tttagtttct gatctatttc ctgaaataaa tattctgcaa tcaagtcttc tctgttttca
     2521 acctttgaaa ctaacagacg catgttgttt ttttcgcgca ccatattaaa gctcgaaatg
     2581 gtatatgcag cataaacaga aaagcctgct atgacaaaca tgtagcgtac aaatgaaaaa
     2641 ttttgccggc ttgtaaaacg aatgtaactg atgaagagaa tgagcgaatt ggtcagaaag
     2701 aatgtggcta caccataatt cacaaaaaca tcagtgtttc gaaacagaat taatacaacc
     2761 agaaaaagtc cttgtgtaat taaaaataaa agaaagttat aaggtaatga gaaaccacta
     2821 tactcagcaa attttactgt cccttcgcag gaaacataaa acgaaaaaag cagaatacct
     2881 attattaaaa atccaatgag gctgaaaaag gttaattcaa aaacattgtt tacgttgaaa
     2941 gaaatttttg agttaagaat taatccgctt ataagataat tgataaaaac agaaaacaag
     3001 aaagtaaaca acagatttcc gattacaaca attgaaagta atgttggact ttgtctgata
     3061 acttttattt ttcttcttct taaatgaagg taattgtaaa cgaacagaat gacacaacaa
     3121 aaaacagatg cactaagcaa aaaatcaccc aatgaattca gaaagtatga tgttgcataa
     3181 aatttaggat taaatagcgg catgccataa agaaattcag gaaaacggaa tgtcagcatg
     3241 agccatttga tgaaaactaa aagactaatt attacaaacg acactaatgg attttttaat
     3301 aaccactgtc ttgcatagag atagaatgaa attaataaaa ggaaaaatcc ggctaaatac
     3361 aatgcagcaa tgtaccacaa gcgacccgga tcgctcatta tcggattgta ttttatactg
     3421 aataaataat ttccatcaga gcttattacc ggataagatc ctgttgccgg tgagggtaac
     3481 agaagggcat gttctggaat tcccaaaaca ggattaaact cattgcgcaa atacttattt
     3541 tgaaaatcat agcctgcacg tatgggcaac aaaccaatga tggtaatatt tcttcgttgg
     3601 taacgaaaca attcatacca accatttttt agttgaataa gttgttggtt gattccgttt
     3661 ttaacttgat tgtatgagaa acttacagca ttattatccc aataaattaa tgagtcgttg
     3721 agatatacaa agctgtagaa agcactaaca ggtttttgcg aagtaagcaa cagactgtcg
     3781 tcaatggcag aataacttgt ttggcgcgaa agcatattac caagccattc aacatatttg
     3841 tttgcccggt tttcttcttc atgtaaagtc ttttttgcaa aaactgttat cctgataaag
     3901 tcaagatcac gtttgtgcat attccactct aatacaatgg cggaaaatac cattgccaaa
     3961 gaaagaataa gtagaaaata cctgaagttg cctttgatca catcccgtaa ggttaaacta
     4021 aacaaaagta tttaatcata cggaaaatca tcaaaatagt tatggtaata agccctccaa
     4081 ggcttatttt tcaggaaagc ctctgctgcg cataagtgct ttaacgtcag ggtcgtgacc
     4141 tctgaaatta cgatagccta tactttcgtc aattgaagca ccaacactaa acacattacg
     4201 ataaagtttt tcggcagtct ttttatcata taaactacct gcttctgtaa atgcttcagt
     4261 tgcatcagcg ctgattacat ctgaccatag ataactgtaa tagcccgctg catattcatc
     4321 acttgaaaaa atatgcccaa attgtggcag tcggtgacgc atcacaattt ccgaaggcat
     4381 attcagtgca gctaaggttt ctttttcaaa tttatcagga tcagttactt tatcacctgc
     4441 aagatgtatt ttcatatcta ccaatgcact cgacagatat tctgttgttt caaatccctg
     4501 attgaatttt gaagatttgt tgagtttttc gagcagactt tgtggaagag gttgtccggt
     4561 tttagcattt attgcatact tctgcaacac ctctgatgtt ggcaaccaac gttcaagtac
     4621 ttgtgatgga aactcaacat aatctctggc cacattggtt cctgaaattg tcggataagt
     4681 aacatcagaa cacaaaccat gcaatgcatg tccgaattca tgaaacagag tagtggcatc
     4741 atcccatgaa ataagtacgg gatcattacc ggaacctttt atgtaattgc aattgttgga
     4801 tacaatagtt gtaacatttc cactcagttt ttcctgttcg cgataggctg tcatccatgc
     4861 accggaacgt ttacctgtac gtgcataggg atcaaaatac cagatcccct ttaacttatg
     4921 tgttgtctta tcttcaactt tatatactgt aacatcagga tgaaaaacag gaacgtcatg
     4981 aacctgagta aatgaaagat tgaataattc tcctgcaaca aaaaacattc cttctctaag
     5041 tttctccaac tgaagatatt gtttcacctc attctgatcg agatcatatt ttgattttct
     5101 tacttgctct gcgtagtagc gataatccca tggctcaatt ttaattcctg cattttcttt
     5161 atcggcaatt gcctgcatgt cggcaacttc ttcttttact ctggcaatag cagcaggcca
     5221 aacagattcc atcaatttca tagtagcttc aggtgtttta gccattttgt ctgaaagttt
     5281 ctgatgtgca aatgttgcaa atcccatcag ctttgctctt tcataacgta attttaaaat
     5341 ttcaggaata atcttgttgt tatcgttggc atcgccattg tcgccacggt ttataaacat
     5401 tgtccaaact tgtttacgta attcgcgatt gtttgaaaaa gttaaaaaag gctcaacagc
     5461 agaacgtgta ttggcaacag tccatgttga tttaccacgc tcttttgcat tttttgcata
     5521 cgctgatttt atgtcatcag gtaatcctgc caaatcggat tcatttttaa tttctatata
     5581 tttttcattc tcatctgcta attgattttt agaaaattga gtaaacaaag tggccaatcg
     5641 tgaattgata ttagccacct tagctttatc agcatcatta agtttagcac ctgcaaggac
     5701 aaaattgttg taataacgtt ttaacaactg atgttgttct acactatatt ttttcccaac
     5761 agtatcgcta cacacttttt ctattcttaa aaacagcggt ttattttgaa taacttcatc
     5821 gttaaatgct gcaatctgtg gttcaatttt ttcctgaata gcagtcatca caggtgtatt
     5881 cagattcgat gaccatatat cataaatagc tcttactcta tttaaagtag ctcctgcttt
     5941 ttccattgct tcaatggtat ttttaaaatt tggagcttct ttgtttgaag ctattttctg
     6001 aatctctgct tttttctctg caaaagccgc ttcaaaagca ggaataaaat attcttcttt
     6061 aactttatca aatgccggaa caccattgta tggccctgtc cagggttgta ataatggatt
     6121 agccgattgt tcttttaaat ctttgttttc agcaaaagaa gcagaagcta tactcatgat
     6181 tgtaagtatt aaaattgatt tctttttcat catacagtaa ttaatgattt tgaatttgta
     6241 ttaatctctt ggctaaggta gaaaaatcaa accacacaga acgaccatt
//
LOCUS       DAOOOI010000107         6284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2390_length_6284_cov_18.630117, whole genome shotgun sequence.
ACCESSION   DAOOOI010000107 DAOOOI010000000
VERSION     DAOOOI010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2390_length_6284_cov_18.630117"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1203
                     /gene="serC"
                     /locus_tag="PKX59_09895"
     CDS             139..1203
                     /gene="serC"
                     /locus_tag="PKX59_09895"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOZ91218.1"
                     /translation="MRKHNFGAGPGVLPEAAIKKTQESLFDFNNSGMGVMEISHRSKD
                     FDAVLQKAKSLVKELLHVPDTHEVLFLQGGASTQFAMVPMNFLKKKAAYIDQGAWSNK
                     AIKEAKLFGEVEVIASSKDKNYSYQPKGYTIPADCDYLHICSNETIHGTQVKNFPASP
                     IPVICDMSSDIFSRPVDVSKFDMIYAGAQKNMGPSGVTLVIIKKDIVGKSEKVMPTML
                     NYKTHLENDSLYNTPPVIAILVCMHVLEWLKSIGGVEAMQKINQQKFDMFYNEVDSNP
                     LFTGTAAPEDRSPMNACFLLKDASMDEAFLAKAKEAGIVGIKGHRSVGGFRASMYNAL
                     PVESVKVLVQVMKDFAKANG"
     gene            1222..2193
                     /locus_tag="PKX59_09900"
     CDS             1222..2193
                     /locus_tag="PKX59_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016193467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ91219.1"
                     /translation="MIKILANDGIDAAGKKIFDSAGFSVSTDKVPQEKLAEALKNFDA
                     ILVRSATTVRTDVIDACPNLKVIGRAGVGMDNIDVKYARDKGLAVINTPAASSQSVAE
                     LVFAHLFTGVRMLHDANRNMPACATKETFDKLKKSYSAGIELKGKTIGIIGAGQIGKC
                     VAAIAVGLGMNVLFHRLNNEDVVVDMDFHPALGQKKITLTFKCVSFTKLLIESDFITM
                     HVPFPKGSAPVIGKKELDLMKNGVGIVNTARGGVISEADLIDALNSGKVAFAGIDVFE
                     GEPLINQTLCAHPKVTLSPHIGGSTKEAQERIGIELAEKVVEYFTKK"
     gene            2221..3465
                     /locus_tag="PKX59_09905"
     CDS             2221..3465
                     /locus_tag="PKX59_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOZ91220.1"
                     /translation="MAILKPFKGIRPKKELVKRIACRPYDVLDEKEARAETKGDKISF
                     YHVIKPEIDFPDDFDHYAPEIYKKGKENFEAMFKEGVFFQDSKDCYYIYAQSMDGRKQ
                     YGIVGCAAVDDYVNDVIKKHELTRPDKEEDRKNHVRVSGLNYEPVFFAYPQVRKLDEL
                     VEHITSAAPEYDFVTDDHIGHQFWIVDNDSDINIITEEFAKLKNTYVADGHHRTAAAA
                     LVGKERREKNPAHTGKEEYNFFLAVHFPDNQLKIMDYNRVIKDLNGMNSNQFIDKLKE
                     NFVVEEKGSDEYRPAALHEFSMYLEGKWYKLNAKPGTFNDKDPIGVLDVTILSDKVLA
                     PMLNITDLRKDKRIDFVGGLRGLGELKRRVDNGEMKVAFALYPVTMQQLIDIADNNLI
                     MPPKVTWFEPKLRSGLVLHSLD"
     gene            3581..4993
                     /locus_tag="PKX59_09910"
     CDS             3581..4993
                     /locus_tag="PKX59_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ91221.1"
                     /translation="MMHDDDYEGFDDQSENLGNSVQRFEEMLRNHETYFFDADSLIKI
                     TDFYLEKFDYDKAMEVIKYGQNLHPDNYAFKAKEAYITHLQGNSEEALEMIDQLLTVN
                     PKNEDLYLIKGLIYNALGMFQEGRKTFKDLLSFTEFKEDVYFHVAQTYLQTFDYHGAI
                     HYFKNCLFENKKNEVAADELSECFDRAGRASEGITYFQALIDQDPYNALFWFHLGQMY
                     QCCVEYEKAINAYDYCILIDENMDDAYIQSGQCHMLSGNYEKAIQSFKQSFEYNTPEA
                     FVYDSIAQCYFEMGNHEESRSYFKKAVKSNPSFHSSWYGIGMTLSEEERWYEAIHYLK
                     KAIELNGDISDYWFLLGDCERELGNLEEADEAYRKVIEMDPDFLDIWYEYPEFLMEQN
                     RTDEALQYAYLAVKYFPNDIAVHLRYIILQYRNGNIKAAYSLLSAAAKGEDDLREIIE
                     EIYPELLNDSNIQKILEQNN"
     gene            4999..5784
                     /locus_tag="PKX59_09915"
     CDS             4999..5784
                     /locus_tag="PKX59_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HOZ91222.1"
                     /translation="MNFELSQMPSRSTKPRENGLTMVMDKGLSLRETEDFISSSGEFT
                     DLVKLGFGSSLITPNLDKKLALYKEAGIPVYFGGTLLEAFLIRDEFDKYLKVLSKYKM
                     EYAEISDGSIVIPHKIKCDYIKKLAKHVTVISEVGSKEEGIIIRPNIWIQMMERELEA
                     GVWKVIAEARESGTVGIYRPNGKAHVMLINKIISRVPADKIIWETPQKSQQVYFIKQF
                     GTNVNLGNIAPSEVIALESLRLGLRSDTFFHFLDKESINRFTN"
     gene            5882..>6284
                     /locus_tag="PKX59_09920"
     CDS             5882..>6284
                     /locus_tag="PKX59_09920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOZ91223.1"
                     /translation="MTASTNINLKKLLIIILLAGFVAKIAIGFHLKDSFLNRGNSKTN
                     LNALACNLYVNGEYSLVQGIPSVDYEPLYPALMSISYAITGYNWLGLTILQAVLHLIT
                     SLLLFKIGTRLFHPIAGFFAACYHAFYPYLFT"
BASE COUNT         2093 a         1091 c         1245 g         1855 t
ORIGIN      
        1 atcagaaata gccataacac tgatggtcag aaaattacac ctgtaaaaga ttctgagctg
       61 cggaagtagt ttattggctt taaagagcta attttgccct ccgaaatatc agataaaaaa
      121 caatttaata tattaactat gcgtaaacat aattttggtg ccggcccggg ggtattacct
      181 gaagctgcaa taaagaaaac acaagaatca ctttttgatt ttaacaacag cggaatgggt
      241 gtgatggaaa tttcgcatcg cagtaaagat tttgatgctg ttcttcaaaa agctaagtcg
      301 ctggtaaaag aattacttca cgtgcccgac acacatgaag tgcttttctt acaaggaggt
      361 gcaagtacac agtttgccat ggtaccaatg aactttctta aaaagaaagc tgcatatatt
      421 gatcagggtg catggagtaa caaagctatt aaagaagcta aactctttgg cgaagttgaa
      481 gtcattgcat caagtaaaga caaaaattac tcctatcaac caaaaggata cactattcct
      541 gccgactgcg actatctgca tatctgtagc aatgaaacca tccacggcac tcaggtaaaa
      601 aatttccctg caagccctat tccggtaatc tgcgatatga gcagcgatat tttttcacgt
      661 cctgttgatg tttcaaagtt tgacatgatt tatgctggtg cacaaaaaaa tatgggccct
      721 tcaggagtaa cacttgtcat cattaaaaaa gacattgttg gtaaatccga aaaagtgatg
      781 ccaactatgc tcaactataa aacgcatctt gaaaacgatt cattgtataa cactcctcct
      841 gtaattgcca ttcttgtttg tatgcatgta ttggaatggt tgaaatcaat tggtggtgtt
      901 gaagcaatgc aaaaaataaa tcaacagaag tttgatatgt tttataatga agttgacagc
      961 aatccattat ttacagggac agcagcaccg gaagatcgtt ctccaatgaa tgcttgtttt
     1021 ctattaaaag atgcatctat ggacgaagct ttcttagcaa aagcaaaaga agccggcatt
     1081 gtaggtatta aaggtcatcg tagtgtaggt ggttttcgtg ccagtatgta caatgcactt
     1141 cctgttgaaa gtgtaaaagt attggtgcag gtgatgaaag attttgcaaa agctaacggt
     1201 taaataaaaa aatattcagt tatgattaaa atactagcca atgatggcat tgatgctgca
     1261 ggaaaaaaaa tatttgattc agcaggattt tcagtttcta cagataaagt acctcaggaa
     1321 aaacttgcgg aagcattaaa gaattttgat gctatattag taagaagtgc tacaactgtt
     1381 cgcacagatg ttattgatgc atgtccaaac cttaaagtaa ttggtcgtgc cggtgtaggt
     1441 atggataata ttgatgtaaa gtatgcccgc gacaaaggac ttgcagtaat caataccccg
     1501 gcagcatcat cacaatcggt tgcggaattg gtttttgcac atttatttac aggagtacgt
     1561 atgctgcatg atgccaaccg caatatgcct gcctgcgcta ctaaagaaac attcgataag
     1621 ctaaaaaaat cttactctgc aggaatagaa ttaaaaggga aaacaatagg cattatcggt
     1681 gccggacaaa ttggtaagtg cgtagctgcc atagccgttg gtttgggaat gaatgttttg
     1741 tttcacagac tgaacaacga agatgtggta gtagatatgg attttcatcc tgccttagga
     1801 cagaaaaaaa taacactcac ttttaaatgt gtttcgttta caaaactcct tattgaaagt
     1861 gattttataa ctatgcatgt gccttttcca aaaggaagtg caccggttat cggcaagaaa
     1921 gaacttgacc tgatgaaaaa tggtgtaggc attgttaaca ctgcccgtgg tggtgtaatt
     1981 tcagaagccg atttaattga tgcactcaac tcagggaaag tagcttttgc aggaatagat
     2041 gtttttgaag gcgaaccatt aattaaccaa acactttgcg cacatcccaa agtaactttg
     2101 tcaccacaca ttggtggttc aaccaaagaa gcacaggagc gcataggcat tgaacttgct
     2161 gaaaaagttg ttgaatattt tactaaaaaa taaaatcaga attaaaattt accaaaacgt
     2221 atggcaatat taaaaccatt caaaggaata agaccaaaaa aagaattagt aaagcggatt
     2281 gcttgccgcc cttatgatgt acttgatgaa aaagaagcac gtgcagaaac caaaggtgat
     2341 aaaatttctt tctatcatgt cataaaacct gaaattgatt ttccggatga cttcgatcat
     2401 tatgcccctg aaatttataa aaagggcaaa gaaaatttcg aagccatgtt taaagaaggt
     2461 gtgttttttc aggacagcaa agactgctac tacatttatg cacaaagcat ggacggccgt
     2521 aaacaatatg gcattgttgg ctgtgcagcc gttgacgatt atgtgaatga tgttatcaag
     2581 aaacatgagc ttacacgtcc tgataaagaa gaagaccgta aaaatcacgt gcgtgtttca
     2641 gggttaaatt atgagcctgt gttttttgca tatcctcaag ttagaaaatt agatgagttg
     2701 gttgaacaca ttacctctgc tgcacctgaa tatgattttg taactgacga tcatatcgga
     2761 catcagtttt ggattgtgga taatgattcc gacataaaca taatcacaga agaatttgca
     2821 aaacttaaaa acacttatgt ggctgacgga catcaccgca cagctgctgc agcattggtt
     2881 ggtaaagaaa gacgcgaaaa aaatcctgca cacacaggca aagaagaata caactttttt
     2941 ctggcagtgc attttcccga caatcaatta aaaataatgg attacaacag ggtcatcaaa
     3001 gacctgaacg gtatgaattc aaatcagttt attgacaaac tgaaagaaaa ttttgttgtt
     3061 gaagaaaaag gaagtgacga atatcgtcct gctgccttac atgaattcag catgtatctt
     3121 gaaggaaaat ggtataaatt aaatgctaaa ccgggcacct tcaacgacaa agatccaatt
     3181 ggtgttttgg acgtaactat tttgtccgac aaagtattag cacctatgtt aaacatcaca
     3241 gaccttcgca aagacaaaag aattgatttt gttggaggac ttcgtggtct cggtgaatta
     3301 aagagaagag ttgataatgg agagatgaag gtagcctttg cactttatcc tgttacaatg
     3361 cagcaactta ttgatattgc tgacaataat cttattatgc caccaaaagt tacctggttt
     3421 gagccaaaac ttcgtagtgg cctcgtactg cattcattag actaattttc ttacagtcaa
     3481 gtatttaaca acctgctgtt gactgtctgc ctgacaggta ctgcaggttg tttcatattt
     3541 tttacctttg acctcaattt gagagaacca atagaagctt atgatgcatg acgatgacta
     3601 tgaaggattt gatgatcaaa gcgaaaattt aggtaacagc gttcagcgtt ttgaagaaat
     3661 gctgcgcaat catgaaacct atttttttga tgccgattcg ctgataaaaa ttactgactt
     3721 ttatttagag aagtttgact atgataaggc tatggaagtc ataaaatatg gtcagaacct
     3781 tcaccccgat aattatgctt ttaaagcaaa agaagcatac attactcatt tacaaggcaa
     3841 ctcagaagag gcgcttgaaa tgattgatca gctacttacc gttaacccca agaacgaaga
     3901 cctttatctt ataaaaggtc ttatatataa tgcattgggt atgtttcagg aaggacgtaa
     3961 aactttcaaa gacctgttgt catttacaga gtttaaagaa gatgtttact ttcatgttgc
     4021 acaaacatac ttgcagacat tcgattatca cggtgccata cattatttta aaaattgtct
     4081 tttcgaaaac aaaaaaaacg aagttgctgc tgacgagctg agcgaatgtt tcgaccgtgc
     4141 aggaagagct tcagaaggca tcacctattt tcaggcatta attgatcagg acccttacaa
     4201 tgcattgttc tggtttcacc tgggacaaat gtatcagtgt tgtgttgaat atgaaaaagc
     4261 catcaatgca tacgactatt gtattctgat tgacgaaaac atggacgatg cttacataca
     4321 aagcgggcag tgccacatgt tgtcaggtaa ctacgaaaaa gcgattcagt ctttcaaaca
     4381 aagttttgaa tataacacac ctgaggcatt tgtttatgac agtatagcac agtgttattt
     4441 tgaaatggga aaccatgaag agtcgcgtag ttattttaag aaggctgtaa aaagtaatcc
     4501 atcttttcac agttcatggt atggcattgg catgacattg tcggaagaag aacgctggta
     4561 cgaagctatt cactacctta aaaaagcaat tgaactaaac ggtgatataa gtgattattg
     4621 gtttttactc ggtgattgcg aacgtgaatt aggcaactta gaagaagctg atgaagctta
     4681 tagaaaagtg attgaaatgg accctgattt tttggatatc tggtatgaat atcccgaatt
     4741 tttgatggaa caaaaccgta ctgatgaagc actgcaatat gcatatcttg ccgtaaaata
     4801 ttttccaaat gacattgcag tacatctgcg atacatcatt ttacaataca gaaacggaaa
     4861 cataaaagct gcatatagct tgttgtcggc tgccgctaaa ggcgaagacg atctcaggga
     4921 aattattgaa gaaatttatc ctgaactttt gaatgactct aacattcaga aaattttaga
     4981 acaaaacaat tagtggcaat gaattttgaa ttgagtcaaa tgccttcacg aagtacaaaa
     5041 cctcgtgaga atggcttaac tatggtgatg gataagggcc ttagccttcg tgaaacagaa
     5101 gattttattt cctcctcagg cgaatttacc gatttggtaa aattaggttt cggcagtagt
     5161 ctcattactc cgaatctgga taaaaaactc gctctttata aagaagccgg aataccggtt
     5221 tattttggcg ggactttgtt agaagctttt cttatcagag atgagttcga caagtacctg
     5281 aaagtattat caaaatacaa aatggaatat gctgaaatat cagatggctc catcgtaatt
     5341 ccacataaaa taaaatgcga ttacattaaa aaacttgcta aacatgtaac agtaatttcc
     5401 gaagtaggct caaaagaaga aggaattatt attcgtccta acatttggat tcaaatgatg
     5461 gaacgtgaac ttgaagccgg tgtttggaaa gtaattgcag aagcacgcga aagtggtaca
     5521 gtgggtattt atcgtcctaa tggaaaagca cacgtaatgc ttatcaataa gataatctca
     5581 agagtgcctg ccgataaaat aatctgggaa acaccacaaa aatcgcaaca ggtttatttc
     5641 attaagcagt ttggcaccaa tgtgaattta ggcaacattg ctcccagcga agttattgct
     5701 cttgaatcgt tgcgtttagg gttaagaagt gacacttttt tccattttct tgataaagaa
     5761 tctatcaata gatttaccaa ttaaacttat tgcttttcag gcataatcgt gccttgtaaa
     5821 gttgtacttt tgcagccgcc caaaagggta taatttcata tttccatttc ctttacattc
     5881 attgactgca agtacaaata taaatttaaa aaaactgctg attattattc tattggcagg
     5941 gttcgttgct aagattgcta tcggatttca tcttaaagat tcctttttga atcgtggcaa
     6001 ttcaaaaacc aatcttaatg cacttgcatg taacctttac gttaacggtg aatacagtct
     6061 tgttcaggga attccttctg ttgactatga acctttgtat cctgcattaa tgagtatcag
     6121 ttatgcaatt acaggatata actggttagg tctgaccatc cttcaggctg tattgcatct
     6181 catcacctct cttctgctat ttaaaatcgg caccagactt tttcacccca ttgcaggatt
     6241 ttttgcagca tgttatcatg ctttttatcc atatctgttt acat
//
LOCUS       DAOOOI010000108         6196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2427_length_6196_cov_29.669598, whole genome shotgun sequence.
ACCESSION   DAOOOI010000108 DAOOOI010000000
VERSION     DAOOOI010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6196
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2427_length_6196_cov_29.669598"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..4499)
                     /locus_tag="PKX59_09925"
     CDS             complement(132..4499)
                     /locus_tag="PKX59_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009411031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOZ91224.1"
                     /translation="MLEKIISFSIKNKLIIAMMTLALIIWGVWSAGRLPIDAVPDITN
                     NQVQIITLCPTLAGQEVEQLVTYPIEQSIANLPDLEELRSISRFGLSLITVVFKENVD
                     VYFARQLINERLNEAVENIPAGVGKPEMGPVSTGLGEVYQYIIHPKKESENKYSAMDL
                     RTMQDWIVARQLYGTPGIAEVNSFGGKLKQYEVAVNPSRLLAMGVTIPDIFSALQKNN
                     ENTGGAYIDKKPNAYFIRGIGLIGSFDDIKNIVVKQNPNGIPILIKDVAEVQFESAIR
                     YGALTYNGEKDVVGGIVMMLKGYNSAEVVNRVKEKMSTIQKSLPDDVVIEPFLDRTDL
                     VKRAISTVEKNLIEGALIVILVLVLFLGNLRAGLIVASAIPLSLLFALGMMNVFGVSA
                     NLMSLGAIDFGLIVDGAVIVVESSLYFLEHRKNMLRLTQNEMDDVVENRTKRMMSSAV
                     FGQIIIMIVYLPILSLIGIEGKMFRPMAQTVSFAIIGALLLSLTYIPMMSALFLPKQL
                     SHKKSFNDKMMDFLQSKYTPLLNTAIRFKRAVVASAVVIFAIAVFVFSRMGGEFIPTL
                     QEGDYAFHCILPQGTSLSQSIETSMQCMREIKKFDEVKMVVGKTGSAEVPSDPMPTEA
                     TDMIIVLKPKSEWKRKISYDELADEIMEKLEVIPGVFFEKSQPIQMRFNELMTGIRQD
                     VAVKIFGENMDTLLYYANRVNVVVQKVQGATEPSVERVAGLPQIVIRYNRSQIANYGL
                     SIEDVNHIVSTAFAGGKAGVVFENERRYDLMVRLDSASRNNLDDVGRLLIPVSNGMQI
                     PLTQVADVKLELGPAQISRENGRRRIVVGFNVKDRDVASVVEEIESQLAEKVKLPEGY
                     YYTYGGTFENLQAASKRLMIALPIALALIFVMLFFTFKSAKQALLIYTTIPMSAIGGV
                     FALLLRDMSFSISAGVGFIALFGVAVLNGIVLIGTFNQLKQEGVEDILERIKEGTRSR
                     LRPVLMTATVASLGFLPMALSQSAGAEVQKPLATVVIGGLITATLLTLFVLPLFYLFF
                     SSEKKVKVEGNNFSGVMPIILLILSAMLLPTISSAQTNVTLEQAISLAMKNNLHLQAQ
                     QLNVQSYESLKKSSFELPKTEVNFQYGQYNSIKKDNGINIQQTIPMPGYFVTKARLNN
                     AELKGAQLQQNIAIKQIISQVSSAYYEIVFLQQNEKELQKLDSIYSGFVQAATLRYTS
                     GESNLLEKTTAETKRGEVQLKVIRNNTALKIAYSTLEALLNTSNLIVTPISNFVPLVP
                     DNVLDSATITNNFSYQLMLQQAAIAKRTKSAEAAMALPELNIGYMNQSIIGFQEVNGA
                     DVYFNRSKRFTGFSMGITVPLTFFSNVSKVKSFSFRHQELLKEAENSKIELQSQLQNA
                     FATYLQNLNQYNYFKNTAMPNADAIINTSTLAYRNGEINYLEYAAALENATAIRTGFL
                     QSIHDVNQTVININSLTNK"
     gene            complement(4593..4964)
                     /locus_tag="PKX59_09930"
     CDS             complement(4593..4964)
                     /locus_tag="PKX59_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91225.1"
                     /translation="MFSSVTIHWVFRKFDLLKIAAYIFSIYFLALSVMPCTDNDALDI
                     FSDLRHSSQSTSANDDHNHEAETCSPFCICACCGHTAATQFTFLYFTTCTAVSNVQFN
                     PYKILLSTESYIGIWQPPKIS"
     gene            5110..>6196
                     /locus_tag="PKX59_09935"
     CDS             5110..>6196
                     /locus_tag="PKX59_09935"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOZ91226.1"
                     /translation="MNHKLRLFLTAFALFFTTMLHADEGMWLLSTLKQLNEADMQKMG
                     FKLTADDIYNINKSSMKDGIIHFGGGCTGEIVSPDGLILTNHHCGLDYIQSLSSLDHD
                     YLTNGFWATARDKELPAPGLSVKFLIRMEDVTQKVKAEISDTLSEADLQAKLPGIYSK
                     LTKEATAGNEFYTADVRAMYGGNEYFLFVYERYEDVRLVGTPPMSIGNFGGDTDNWMW
                     PRQTGDFSMFRIYTTPDGKPAKYAKENVPLKSKYYFPVSIKGVKEGDFAMIMGYPGRT
                     DRYATSYSIENGLDITSPSLVKLRTKRLDVINNAMADNDAIRIKYQAKRNQVSNYWKY
                     FIGQQKQLKRMKVVEQKRNLENQFTTWV"
BASE COUNT         1904 a         1257 c         1055 g         1980 t
ORIGIN      
        1 actttcatgt gcatgtgcat caacagggtt ttcggcttca ccggttgtgt tttttgtgct
       61 gcatgaacct gtcagcagcg aaattaatat gatatataaa taatacttta tcgtttgcat
      121 tgttctgatt tttatttatt ggttaaagaa tttatgttga tgactgtttg atttacatcg
      181 tgaatacttt gcagaaagcc tgttcgtatt gctgttgcat tttcaagtgc tgctgcatat
      241 tcaagataat ttatttcacc gtttctgtat gctaatgttg atgtgttgat tattgcatcg
      301 gcatttggca ttgcagtgtt tttaaaataa ttgtactgat tcaggttttg cagatatgtg
      361 gcaaatgcat tctgtagctg actttgcaac tctattttac tgttctccgc ctctttaagc
      421 aattcctgat gtctgaatga aaatgatttt actttactga cattactaaa gaatgtcagt
      481 ggtacagtga tgcccatgct aaaccctgtg aatcgcttgc ttctattaaa ataaacatct
      541 gctccattca cttcctgaaa gccaatgata ctttgattca tataaccaat attcaattca
      601 ggtagtgcca tggctgcttc tgcagacttt gttcttttag caattgctgc ttgttgtaac
      661 atgagttgat aagaaaaatt gtttgtaatc gtagctgaat ccaaaacatt atcgggtaca
      721 agaggcacga aattacttat cggtgtcaca ataagatttg aagtatttaa aagtgcttct
      781 aatgttgagt aagcgatttt aagtgctgta ttatttctaa taacttttaa ttgcacttca
      841 ccacgtttgg tttctgccgt tgttttttca agcaggttac tttcaccgga agtgtaacgc
      901 aatgttgctg cctgtacaaa gcccgaataa atgctgtcca atttttgtag ctctttttca
      961 ttctgctgta aaaagacaat ctcataataa gcagacgaaa cctgagagat aatttgtttg
     1021 attgctatat tctgctgtag ttgtgctcct tttaactcag cattgttcaa tcgtgctttt
     1081 gtaacaaaat atccgggcat cggaatggtt tgttgtatat ttattccatt atcctttttt
     1141 atactgttgt attgtccgta ttgaaaattt acttctgtct ttggcaactc aaaacttgac
     1201 ttctttaaag actcatacga ttgtacattt aattgttgtg cctgaagatg taaattgttt
     1261 ttcattgcta atgaaatggc ttgttccaat gtaacatttg tttgtgctga tgatattgta
     1321 ggaagcaaca tagctgataa tatcaaaaga attataggca ttactcctga aaaattattt
     1381 ccttcaactt taactttctt ctctgaagaa aaaaacaaat aaaataaggg tagcacaaaa
     1441 agtgtaagca gtgttgcagt gataagaccg ccaataacta ctgttgccaa aggcttctgc
     1501 acttctgctc ctgcactttg cgacaatgcc attggcaaaa atcctaacga tgccacagta
     1561 gcagtcatca atacaggacg aagtcgtgac cttgttcctt cttttattcg ctccagaata
     1621 tcttcaacac cttcctgctt taactgatta aaagtgccga tgagaacaat tccatttagc
     1681 acagctacac caaacaaggc aataaagcca acaccggcag aaatactaaa agacatatcg
     1741 cgcaacaaca atgcaaaaac accaccaatg gcactcatgg gtatggttgt ataaatcaac
     1801 aatgcctgct tggccgactt aaacgtaaaa aacaacataa caaaaatgag tgctaaggca
     1861 ataggcaaag caatcatcaa tcttttactt gctgcctgaa gattttcaaa tgttccgcca
     1921 taggtataat aatatccttc gggtaatttt actttttctg caagctgcga ttctatctct
     1981 tcaacaacac ttgccacatc gcggtctttt acattaaagc caacaactat tcttcttcgt
     2041 ccgttttcgc gactgatttg tgcagggccc aattccaatt ttacatcagc aacctgagtt
     2101 aatggaattt gcataccatt actgactggt ataagtaacc tgccaacatc atccagatta
     2161 tttctgcttg cactgtctaa acgcaccatc aggtcatagc gtctttcgtt ttcaaaaacc
     2221 actccggctt taccaccagc aaatgccgtg ctcacaatgt ggtttacatc ttctattgac
     2281 aatccatagt ttgctatctg cgaacgattg tatctgatga ctatctgagg caatcctgca
     2341 acacgttcaa cacttggctc cgttgctccc tgtacttttt gtacaactac atttactcga
     2401 ttggcataat aaagtaatgt gtccatattt tctccgaaaa tttttactgc aacatcctgt
     2461 cttatccctg tcatcaattc gttaaaccgc atttgtatag gttggctttt ttcaaaaaaa
     2521 actccgggaa taacttccaa cttctccata atttcatctg caagctcatc ataactgatt
     2581 tttcttttcc actcactttt gggtttaagt acaataatca tatccgtagc ttcagtaggc
     2641 attggatctg aaggcacctc tgcactaccc gtcttgccta caaccatttt cacttcatca
     2701 aactttttga tttcacgcat acattgcatt gaagtttcta tgctttgtga taacgatgtg
     2761 ccctgtggta atatgcaatg aaaagcataa tcgccttcct gaagtgttgg aataaactca
     2821 ccacccattc ttgaaaagac aaaaacagca atagcaaata tcacaacagc gcttgcaacc
     2881 acagctcttt taaaacgaat agcagtgtta agcaacgggg tatatttact ttgtaaaaaa
     2941 tccatcattt tgtcgttgaa agattttttg tgcgaaagtt gtttgggtag aaataatgca
     3001 ctcatcattg gaatgtaggt gagtgaaagt agcagcgccc caataatggc aaatgaaact
     3061 gtctgcgcca ttggcctgaa catttttcct tcaatgccta ttaaactaag tatgggcagg
     3121 taaacaatca taataattat ctgaccaaaa actgcactgc tcatcattct tttggtgcgg
     3181 ttttccacca catcatccat ttcattttgt gtaagtcgta gcatattttt tctatgctcc
     3241 agaaaataaa gcgaagattc aacaacaatt actgcaccgt caacaataag tccgaagtca
     3301 attgctccaa ggctcatcag attcgcacta acaccaaaca cattcatcat tccaagagca
     3361 aacaacagtg acaaaggaat agcgctggct acaattaaac ccgctcttag atttccaaga
     3421 aacaaaacca acacaagaat aactattaat gccccttcaa tcaggttctt ttctactgta
     3481 gaaattgctc tttttaccaa atcggtgcga tcaagaaatg gttcaataac tacatcatca
     3541 ggcaatgatt tctgtatagt actcattttc tcttttacac gattcactac ttctgcactg
     3601 ttgtagcctt ttagcatcat cactatgcca ccaacaacat ctttctctcc attatatgtc
     3661 aatgcaccat agcgaattgc actttcaaac tgaacttcgg ctacatcttt aatcagaatt
     3721 ggaataccat ttggattttg tttgactaca atattcttta tatcatcaaa cgaacctatt
     3781 aaaccaatac cccgaataaa ataggcgttt ggttttttat caatatatgc ccctcctgtg
     3841 ttctcattat tcttttggag tgccgaaaaa atatccggta tggtaacacc cattgcaagt
     3901 aacctcgaag gatttacagc tacttcatac tgttttagtt ttcctccaaa agaatttact
     3961 tcggcaatgc ccggtgtgcc gtatagctgt cgtgcaacaa tccaatcctg catggtgcgc
     4021 aagtccattg ctgaatattt gttttcgctt tctttttttg gatgaataat gtactgatac
     4081 acttctccca atcctgtact tacaggaccc atctcaggct tgcctacacc tgcaggtata
     4141 ttctcaacag cctcattcaa tctttcattg atgagctgtc gtgcaaaata cacatctaca
     4201 ttttctttaa aaactactgt aatcaacgaa agaccaaagc gcgaaatact tcgcaactct
     4261 tcaaggtcag gaaggtttgc aatactttgt tctatcggat aagtcaccaa ctgctctacc
     4321 tcctgtccgg caagtgtagg gcataatgta attatctgta cctgattgtt ggtaatatca
     4381 ggaacagcat ctattggtaa tctgccagca ctccacacac cccatattat gagtgccagc
     4441 gtcatcatgg caatgattaa tttgttcttt atactgaacg aaataatttt ttctaacata
     4501 tataatcagt taaaattaaa catataactt attgtatgcc attttgcaat agcattaatt
     4561 cacattgcaa aaagcaatgt cataaattta tattaactga ttttgggcgg ctgccagatt
     4621 cctatataac tctctgtaga aagtaaaatt ttatagggat taaactgcac gttacttact
     4681 gcagtgcagg tggtgaaata taaaaacgta aattgtgtag cagccgtatg accacaacat
     4741 gcacaaatac aaaatggtga gcaggtttct gcttcatgat tatgatcatc atttgcagaa
     4801 gttgattgtg atgaatgacg caaatctgaa aaaatatcta atgcatcgtt atctgtacat
     4861 ggcattacag ataaagctaa aaagtatata ctgaatatgt aggctgctat tttcaacagg
     4921 tcaaatttac gaaaaaccca atgaatggtt actgaagaaa acaactgatt tgtgtcataa
     4981 aatgtatgcg aaaattttgg aactaacaga ataaatttgt taccgtctaa tattcctctg
     5041 tcattaacaa gtttttaacg attataagaa taaaaataca gatattcgcc acacaaaatc
     5101 ttttaaatta tgaatcataa attacgactg tttctaacag cctttgcact gttttttact
     5161 acaatgttgc atgccgatga aggtatgtgg ctgctgtcaa ctttaaaaca acttaatgag
     5221 gcagacatgc aaaaaatggg cttcaagctc acggcagatg acatttacaa catcaacaaa
     5281 tcatccatga aagatggtat cattcacttt ggtggtggat gtacaggcga aattgtttcg
     5341 cccgatggat taattcttac caaccatcac tgcggacttg attacattca atcgttgagt
     5401 agtttagatc atgattattt gactaacggt ttttgggcaa cagctcgcga taaagaactt
     5461 cctgcaccag gtctttcagt aaaattcctt atccgcatgg aagatgttac acaaaaagta
     5521 aaggcagaaa tcagcgacac actttccgaa gctgatttac aagccaaact acctggaatc
     5581 tattccaagc tgactaaaga agcaactgca ggtaatgagt tttatactgc cgatgtgcgt
     5641 gcaatgtatg gtggcaatga atattttctt tttgtttacg aaagatacga agatgtgcgt
     5701 ttggttggaa cacccccaat gagcattggt aactttggtg gcgacaccga caactggatg
     5761 tggccacgtc agacaggcga tttttctatg tttcgtatct acacaactcc cgatggaaaa
     5821 cctgcaaagt atgctaaaga aaatgttccg ttaaaatcca aatactattt tcctgtcagc
     5881 attaaaggtg ttaaagaagg cgattttgct atgattatgg gctaccccgg tcgcaccgat
     5941 cgttatgcaa cttcatacag cattgaaaat ggtcttgaca ttacaagtcc atcgttagtg
     6001 aaacttcgta ccaaaagatt agatgtcatc aacaatgcaa tggcagataa tgatgctata
     6061 agaataaaat atcaggctaa gcgcaatcag gtaagcaact actggaaata ttttatcggt
     6121 cagcagaagc aattaaaaag aatgaaagtt gttgaacaaa aacgcaacct cgaaaatcaa
     6181 ttcactacat gggtta
//
LOCUS       DAOOOI010000109         6004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2516_length_6004_cov_10.227937, whole genome shotgun sequence.
ACCESSION   DAOOOI010000109 DAOOOI010000000
VERSION     DAOOOI010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2516_length_6004_cov_10.227937"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PKX59_09940"
     CDS             <1..621
                     /locus_tag="PKX59_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91227.1"
                     /translation="VVANIPDLLDLPYFTSIAYDALVLTEQQAQDLNALYASNNPPIT
                     FQAGNNPLVIADPSAASGKRQMKPGELVLLPVTLDSINCAGWGSNTQKPLGNRYVLTT
                     SEINTLRNTITAYNNYLKTKADENNLAYVDMYAFFKKINNGFPHNGVTYSSEFLKGQF
                     FSLDGITPTGRGSALIANEFIKAINEKYQTRIPQTDANSFPGILFP"
     gene            complement(655..2031)
                     /locus_tag="PKX59_09945"
     CDS             complement(655..2031)
                     /locus_tag="PKX59_09945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ91228.1"
                     /translation="MKVISADDKSATSLSDSAQLRYITFILLYFSQGIPEGLTLFAIP
                     AWLAFNGKSAVEIAAYSATIMIPFSLKILLAPIIEKYTYLPMGRRRPWLLFGQFGIMS
                     GLIALAFVPEPLNNIFVIICVVLCMHVFVMFQDIATDSLVIDIVPVDQQGRANSLMWG
                     SKTIGSSVSLFLGSWLINEYGFASAFVILSVPILVVMFVPLLLRERKGEKLLPWTHGQ
                     TSDEAKQFVVESWKKMFKSFKQVVLLRNSILLLSSVFIALAAIYFINTLLPIFTIQKL
                     GWTNVYFSKIFSSCRLIGGVSGMLVGALVIQRMGVIRFIQSSLILMIVIAILFVMGAP
                     FWHHLNMIIGFITTFCILATLINIGVLALAMQLCWKRVSAIQFTFCMTIFNAGFATGA
                     ALLGWLKPYMDWQQLFLVFACLIAFTMLLFKFMKTSNHVKQVEKLEDQYIDVLKAEGS
                     LLVKSETV"
     gene            complement(2163..3386)
                     /locus_tag="PKX59_09950"
     CDS             complement(2163..3386)
                     /locus_tag="PKX59_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91229.1"
                     /translation="MKTFFNLIFFLFVGMNLKAQTYGCTDSLANNYNANATANDGSCI
                     YNSANVVPLSSNLLPIALDETSGLTFWNNMLWSLNDDADNNLYAIDTGNFNNYTAYPL
                     NEPNIDWEEISQDDDYFYIGDFGNNVSGNRTDLKILKIEKNSLLQNNPNVDTINFSYS
                     TQTNFNPTAINSTDFDCEAFVITADSIYLFTKEWISNHCSVYSLPNKSGSYSANFISS
                     YNVNGLVTGATQIQNSRAVVLSGYSTVMQPFVLLLYDYQGFNFFSGNKRIINVNLPFH
                     QTEGIATVNGLKYYLSNEKLVQTPINIQAKIHEIDLTPYLSHYLNSVSGSFDISENPY
                     AINIYPNPTTDFINVKWNTGVKVARIKIFNISGKEVMNQIVTEKYFQIDIRSFEKGIY
                     FLSVNDSVFRKVVKM"
     gene            complement(3551..4471)
                     /locus_tag="PKX59_09955"
     CDS             complement(3551..4471)
                     /locus_tag="PKX59_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019981575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOZ91230.1"
                     /translation="METKQKEKIKKVAVGLNGHSHHAETEIRLAVQKTYKLYIDGKFP
                     RTESGRYYKLNDKNGKVIANICRASRKDFRDAVVVARKAQGDWQKKSAFNRSQILYRI
                     AETLETRRLQFVSTLGLQGVSETAAQKEVSAAVDMLVYYAGWCDKYQQVFSSVNPVES
                     SHFNFSFPEPTGVVTAIASPEQGLLNLVSLMAPSLAGGNSIIILAAEKFPLTAIDFAE
                     VLNASDVPGGVVNILTGYSKELHTHFSSHMDVNAIVYAGDNSAELKTIQTNAAGNVKR
                     VVVVQNIAEESPYRIMELQEVKTTWHPVGI"
     gene            complement(4490..5965)
                     /locus_tag="PKX59_09960"
     CDS             complement(4490..5965)
                     /locus_tag="PKX59_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOZ91231.1"
                     /translation="MAKRTAKQNTRLKFDTAWQYSAAPETPDHIVLEDKYNLFINGKF
                     VAPQSKKYFDTINPAKETRIASVAEASQADVDAAVKAARTAYDKVWSKMPARERGKYI
                     YRIARIMQERARELAIIESLDGGKPIRESRDIDVPLAAAHFFYYAGWADKLDYALPGM
                     AAQPLGVAGQIIPWNFPILMAAWKIAPALACGNTVVLKPAETTPLTALKLAEIFRDAD
                     LPPGVVNIVTGAGATGAAIVNHPGIDKVAFTGSTDVGKIIQKALAGTTKKYTLELGGK
                     AANIIFEDAAIDQAVEGIVNGIFFNQGHVCCAGSRLFVQESVANEVVRKLKDRMETLI
                     IGDPLDKNTDIGAINSREQLNKIKALVKVGVNEGGDCYQSSCTLPSKGFWFKPTIFTG
                     VAQSSRIVQEEIFGPVLSIQTFRTVEEAVEKANNTPYGLSGGVWSDKGSKVFKVTSKI
                     KAGVLWANTYNKFDPTSPFGGFKESGMGREGGMHGLSAYLK"
BASE COUNT         1909 a         1231 c          971 g         1893 t
ORIGIN      
        1 gtagttgcaa atattcctga cctactcgac ctaccctatt ttacttccat agcctacgat
       61 gctctggtac ttacagaaca acaggctcag gatcttaatg cattatatgc ttcaaacaat
      121 ccaccgataa cttttcaggc aggtaacaat ccattggtaa ttgctgaccc atctgcagca
      181 tcaggaaaaa gacaaatgaa gccgggtgag ttagtgctgt tgccggttac ccttgacagt
      241 ataaactgtg caggttgggg cagcaataca cagaagcctc ttggaaacag atatgtgctc
      301 accacttcag aaatcaatac gcttagaaat acaatcaccg cctacaacaa ttatcttaaa
      361 acaaaagctg atgaaaacaa tttagcttat gttgatatgt atgccttctt taaaaaaatc
      421 aacaatggtt ttccgcacaa tggtgtaact tacagcagcg agtttcttaa aggacagttt
      481 ttttctcttg acggcattac gcctactgga agaggcagtg cacttattgc caatgaattt
      541 ataaaagcta ttaacgaaaa atatcagaca cgcattccac aaacagatgc aaattcgttt
      601 cccggaattt tatttccgta aggtgttttc gattaaaaat taattttttg taaactaaac
      661 tgtctctgat tttaccagca aactcccttc tgcttttaaa acatcaatat actggtcttc
      721 taatttttca acttgtttta catggtttga tgttttcata aatttaaaca acaacattgt
      781 aaaagctatc aagcaggcaa aaaccaaaaa cagttgttgc cagtccatgt aaggcttcag
      841 ccatcccagc agtgcagcac ccgttgcaaa cccggcatta aatattgtca tacaaaatgt
      901 aaattgaatg gcagaaactc ttttccagca cagttgcatg gctaatgcaa gcacaccaat
      961 attaattaat gttgccaata tgcaaaaggt agtgataaat ccaattatca tattaagatg
     1021 atgccaaaaa ggagcaccca taacaaatag aatagcaatt acaatcatta atattaaact
     1081 actttgtata aaacgtataa cacccattcg ctgaattaca agtgcgccaa ccaacattcc
     1141 ggatacacct ccgattaatc tgcacgatga aaatattttt gagaaataca cattggtcca
     1201 gccaagtttc tgtattgtaa aaattggcag taatgtattg ataaaatata ttgcagccag
     1261 tgcaataaaa acagaagaca acaacaaaat agaattacgc aacaaaacaa cttgcttgaa
     1321 tgacttaaac atcttcttcc agctttcaac tacaaattgt tttgcctcat cagaagtttg
     1381 gccatgagtc caaggaagaa gtttctctcc tttacgttcc cgcaacaaaa gtggcacaaa
     1441 catgacaact aaaattggca ccgataaaat tacaaaagca cttgcaaaac catattcatt
     1501 gatgagccaa cttcctaaaa aaagtgacac agaagatcca attgttttgg aaccccacat
     1561 taaactattg gctcttccct gctgatcaac cggaacaatg tctatcacta ggctatctgt
     1621 agcaatatcc tgaaacataa caaaaacatg catacataga actacacaaa ttataacgaa
     1681 aatattattc agtggctcgg gcacaaaagc aagtgcaatt agtcctgaca taattccaaa
     1741 ctgaccaaac aacaaccaag gtcttctgcg tcccataggc agataggtgt atttttctat
     1801 gattggagcc agcaatattt ttaaactaaa cggaatcata atagttgcac tatatgctgc
     1861 aatttcaaca gcactcttac cattgaaagc cagccatgca gggatagcaa aaagtgtcag
     1921 cccctctgga atgccttgtg agaaatacaa taaaatgaag gtaatatatc gaagttgcgc
     1981 actatcggaa agcgatgttg cactcttgtc atctgcagaa atgactttca taaaatttaa
     2041 tttagattgt taggtgttgt aaagttaaaa caaagataat atgtctattg atttaactgc
     2101 aagctaaaat acacaaacca gtaatataaa gtcaacgaac tacaatctgt ttttcaatac
     2161 agtcacattt tgactacttt ccggaatacg ctatcattaa cactcagaaa ataaattccc
     2221 ttttcaaaac ttctgatgtc aatctgaaaa tacttttctg ttactatttg gttcatcacc
     2281 tcttttccgc tgatgttaaa aattttaatt cgtgcaactt tcactcctgt attccattta
     2341 acattaataa aatctgttgt aggatttggg taaatattta ttgcataagg attttcagat
     2401 atatcaaaac ttccggaaac agaattcaaa tagtgactta aatatggcgt taaatctatt
     2461 tcatgaatct ttgcctgaat gtttattggt gtttgtacaa gtttttcatt ggacaaataa
     2521 tattttaatc cattaacagt tgctatgcct tcagtctgat gaaaaggcag attaacattg
     2581 ataattcttt tattaccact aaaaaaattg aatccctgat aatcatataa aagtaaaaca
     2641 aaaggttgca tcactgttga atagcctgaa agtacaactg ccctactatt ttgaatttgg
     2701 gtagcaccgg taacgagtcc gttcacatta taggatgaaa taaaatttgc actataacta
     2761 cccgacttgt ttggaagaga ataaacagag caatgattgc ttatccactc ttttgtaaac
     2821 aaataaatac tgtcggctgt tattacaaaa gcttcacaat caaaatcggt gctgtttatg
     2881 gcagtaggat taaaatttgt ttgcgttgaa taagaaaagt ttattgtatc aacattagga
     2941 ttgttctgca gtaaagagtt tttttcgatt ttcaatattt tcaaatcggt tctgtttccg
     3001 ctgacattat ttccaaaatc acctatataa aaatagtcat catcctgcga aatttcctcc
     3061 cagtcaatat taggctcgtt taaaggataa gctgtatagt tattaaaatt tccggtgtca
     3121 attgcatata aattattatc tgcatcatca ttcagcgacc acaacatatt attccaaaaa
     3181 gttaatcctg atgtttcgtc taatgcaatt ggcaataaat tactactcaa tggaacaaca
     3241 ttggctgagt tataaataca actaccatca tttgcagttg cattggcatt ataattattg
     3301 gctaacgaat ctgtacaacc ataagtttgt gctttcagat tcattcctac aaacaagaaa
     3361 aaaatcaaat taaaaaatgt tttcataaat gtgtttacaa ttcaaataaa tcacttctgt
     3421 cagaagacag cacttgactt caattttgcc aattaatatt tgatcatttc acagctaaac
     3481 gatagccttg gctttcgttc aggtcaaaga tattgcattc atgaatacaa aaatatatat
     3541 tgacccaagc tcaaataccc acaggatgcc atgtggtttt tacttcctga agttccatga
     3601 tgcgatatgg actttcttca gcaatattct gaactactac tacgcgttta acattacctg
     3661 ctgcattggt ttgtatggtc ttcaattcag cactgttatc acctgcataa acaatggcat
     3721 taacatccat gtgtgacgaa aagtgcgtat gcaattcttt actataacct gtaagtatat
     3781 ttacaacacc accgggcaca tcgcttgcat taagcacctc tgcaaaatca attgctgtga
     3841 gaggaaattt ttctgctgcc agaataatta ttgaattacc tcctgcaagt gaaggtgcca
     3901 taagagaaac cagatttaat aaaccttgtt ctggcgatgc aattgctgta acaactcctg
     3961 ttggctcagg aaaagaaaaa ttaaaatgtg atgattctac gggattcaca cttgaaaaaa
     4021 cctgctgata tttatcgcac catcctgcat aataaacaag catatctact gctgcactca
     4081 cttccttttg tgctgccgtt tcactcacac cttgcagacc taaggttgac acaaattgta
     4141 gtcttcgtgt ttccagagtt tctgcaatgc gatacaaaat ctgacttcta ttaaatgccg
     4201 atttcttttg ccagtcgcct tgtgcttttc gtgcaactac aactgcatct ctgaaatctt
     4261 ttcttgatgc acgacaaata ttagcaatta cctttccgtt cttatcatta agcttataat
     4321 atcttccgct ctctgttcgt ggaaatttac catcaatgta aagcttataa gttttttgta
     4381 cagctaacct tatttcggtt tctgcatgat ggctgtgacc attaagtcca actgctactt
     4441 tttttatttt ctctttttgt ttcgtctcca taatgttaat gcgttaaaat tattttaaat
     4501 atgcactaag tccatgcatg ccaccttcgc gacccatgcc gctttcttta aatcctccaa
     4561 atggcgaagt aggatcaaac ttattataag tgttggccca taatacacct gcttttattt
     4621 ttgaagtaac tttaaatacc ttactgcctt tatcagacca tacgcctcca cttaaaccat
     4681 aaggtgtgtt gtttgctttt tcaactgcct cttctactgt tctgaatgtt tgaattgaaa
     4741 gaacaggccc aaatatttct tcctgaacaa ttctacttga ctgtgcaaca ccggtaaata
     4801 ttgtaggctt aaaccaaaaa ccttttgacg gcaatgtgca agatgactga taacagtcac
     4861 cgccttcgtt aacacctact ttaaccaatg cttttatttt attcaattgc tcgcgtgagt
     4921 tgatggcacc tatatcggta ttcttatcta atggatcgcc aatgatgagt gtttccatac
     4981 ggtctttgag tttgcgcaca acttcgttgg caacactttc ctgaacaaac aatcgtgagc
     5041 ctgcacaaca aacatgtccc tgattaaaaa atattccgtt aacaatgcct tcaacagcct
     5101 gatcaatggc agcatcttca aaaatgatgt tagctgcctt gcctccaagt tcaagggtgt
     5161 atttttttgt tgttcctgct aatgctttct gaataatttt tccaacatca gtagaaccgg
     5221 taaaagctac tttatcaata ccgggatgat taacaatagc tgctccagtg gctcctgctc
     5281 ctgtaacaat gttcactaca ccgggaggaa ggtcggcatc tctgaaaatt tctgccaatt
     5341 ttaatgctgt aagtggagtt gtttctgcag gttttagcac aacagtattt ccacaggcaa
     5401 gtgcaggtgc aattttccat gctgccatta atatcggaaa attccatggg ataatttgtc
     5461 cggcaacacc taaaggttgt gctgccattc cgggtaatgc gtaatcaagt ttatcagccc
     5521 atcctgcata ataaaaaaag tgtgctgctg ctaaaggaac atcaatatct ctgctttcgc
     5581 gaataggctt tccaccatct aaactttcaa taatggcaag ttcgcgggca cgctcctgca
     5641 taatgcgggc aatacggtaa atatatttcc ctctctcgcg tgccggcatt ttgctccaca
     5701 ctttatcata agctgtacga gctgccttta ctgctgcatc tacatcggct tgcgatgctt
     5761 ctgcaacaga tgctattcgc gtctcttttg cagggttaat ggtgtcaaaa tattttttgc
     5821 tctgtggtgc aacaaattta ccattgataa atagattgta tttgtcttct aacacaatgt
     5881 gatccggagt ttcaggtgct gctgaatact gccatgccgt atcaaatttc aacctggtat
     5941 tttgctttgc tgttcgcttt gccatttacg aaaatttaag attcaaagat agaaaattca
     6001 tttc
//
LOCUS       DAOOOI010000110         5991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2525_length_5991_cov_18.522911, whole genome shotgun sequence.
ACCESSION   DAOOOI010000110 DAOOOI010000000
VERSION     DAOOOI010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5991
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2525_length_5991_cov_18.522911"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PKX59_09965"
     CDS             complement(<1..638)
                     /locus_tag="PKX59_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HOZ91232.1"
                     /translation="MTNFIPVRVAVLDLYNGEPNEGMRSIRSILERFSKFTVCSFFDV
                     RQKNEVPDLSFDIYISSGGPGCPLPAGEEWEEPFYALWDKLIEHNADTENKKKYAFLI
                     CHSFQMMAHHLGFGVVSERHSPSFGIFPIHKTDDGNNDRIIGRLPEPFYAVDSREWQV
                     TTPDLDKIVHSGGKLLCLEKMRPHVPYERAIMGLRFSDEILGVQFHPEADAEG"
     gene            complement(635..1756)
                     /locus_tag="PKX59_09970"
     CDS             complement(635..1756)
                     /locus_tag="PKX59_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018621374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HOZ91233.1"
                     /translation="MPQFTLGVEEEFQVIDPKTRELRSHMHQIVEGGKVILKEQVKAE
                     MHQSVVEVGTNICKDVSEARKEVTYLRQMVAKLAQKQGLVIGAAGTHPFSRWQDQLIT
                     DHPRYEEIIREMQDAARSNLIFGLHVHVGIDDREIGIRLMNQARYFLPHIFALSTNSP
                     FWEGRNTGFKSFRTKVFDKFPRTGIPDEFSSAAEYDAYIQLLVKTNCMDNAKKVWWDI
                     RLHPFFNTIEFRICDIPMRIEETITLAAIMQGIIAKLYLLMQFNLSFRTYSRALINEN
                     KWRAARYGIDDKLIDFGKEKEVETKLLIEEITEFIDDVVGDLGSRKEVDHVFSMMKRG
                     TGADRQLAVFEKTGDLKKVVDYIISETNIDIPVKKEKKK"
     gene            complement(1801..2799)
                     /locus_tag="PKX59_09975"
     CDS             complement(1801..2799)
                     /locus_tag="PKX59_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91234.1"
                     /translation="MKKIGILYGQENSFPPAVVERINAKNISGIVAESVQVDKVVQGM
                     PMDYAVIVDRISHDVPFYRGMLKNAAICGTAVLNNPFWNSADEKFFNNCLATKIGVPV
                     PKTVLLPSNVHPPDTNENSFRNLKMPLDWDNIFKYIGFPAYMKPFAGGGWKNVYKCND
                     AADFYNKHQETGQLVMLLQEEIVFDQYFRCYCIGGKRIKIMRYEPRNPHHLRYVVDGP
                     APEQRILDVVEEYTFKLCNALGYDINTVEFAIRDGVPYAIDFCNPAPDADRHSVGDEN
                     FNWVIEAVADMCIEKALSHREGQDNLTWGAFVKHAAQGQIVNKQNMDALMESYTVK"
     gene            3081..4181
                     /locus_tag="PKX59_09980"
     CDS             3081..4181
                     /locus_tag="PKX59_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91235.1"
                     /translation="MRIIFLIIVILHGLIHLLGFVKGIGLKEVKELTLPISKPLGFIW
                     LASAFIFLAYGISFIVNYKYSWLIGFLAIAVSQILIILIWKDAKFGTIPNALIFIVSF
                     FSWGYYSFQKCILEETQQLLSQNKIIENKIMTESEIQLLPYPVKNWIRHSGALGRPLI
                     SIGKVVQSAQLKLNPGQENWMNATAIQYTTIDKPAFIWAVDVKMNNIVSFKGRDKFEN
                     GNGEMLIKMNSLINIVNEQGEKLSEASLQRFLGEMVWFPSLALSPYISWEQINDTTAR
                     ATMSYKGTSGSGTFYFNDKSDITKFTAFRYKDNNAVAKKHEWSISISDYKTFEGVKVP
                     AIMSSTWKLDAGDWTWLKLEVLDIKYNDESSK"
     gene            4307..5101
                     /locus_tag="PKX59_09985"
     CDS             4307..5101
                     /locus_tag="PKX59_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOZ91236.1"
                     /translation="MEKNYQYQNIVRSRIATFDIFDIGLRKHHINAILEFDVTESRAQ
                     LHELRKNGTVISFNAWLIKVIGTVLKKHPEAASYIYSRKRLIMFNDINVSLIVEKEIG
                     NSKVPIPIVIEKTNEKSAYDITIEIENAKSQKLDIDNIVLKSRANTFQTLYYHLPGFF
                     RKLFWRILLRNPKLAFKTMGNVAVTSIGMIGRINGWFIHKSVHPISFGIASIIKKPIV
                     IENKIEIREILNMTILVDHDIIDGAPMVRLLNDLTNYIETGKEINA"
     gene            5319..>5991
                     /gene="rhuM"
                     /locus_tag="PKX59_09990"
     CDS             5319..>5991
                     /gene="rhuM"
                     /locus_tag="PKX59_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HOZ91237.1"
                     /translation="MNTIELYRSSNGETQIEVRFEDETVWLSLNQIAELFGRDKSVIS
                     RHLKNIYKESELNYSSTAAKNATVQTEGKRNITREIDYYNLDAIISVGYRVNSKQGTQ
                     FRIWATNRLKEYLIKGYSINQKRLQELNQIVQFIAQSENNTNKISETKGLLNILTKYA
                     KSFILLNQFDSNAIELKNLDENITYEIDYNEAVISINELKNQLIEQKEATELFGNQKD
                     QNFEGI"
BASE COUNT         1976 a         1163 c         1009 g         1843 t
ORIGIN      
        1 ccttctgcat cggcttccgg atgaaactgc acgcccaata tttcatcact aaatcgcaat
       61 cccattattg cacgctcata cggaacatgt ggtctcattt tctctaagca aagaagtttg
      121 ccaccactgt gcacaatttt atcaagatcg ggagttgtca cctgccattc tcttgagtca
      181 acagcataaa acggttcggg aaggcgacca ataattctgt cattatttcc atcatcggtt
      241 ttatgtatag gaaaaattcc gaacgaaggc gaatgtcttt cgctcactac gccaaagcct
      301 aaatgatgtg ccatcatctg aaatgagtga catatcagaa aagcatattt cttcttattt
      361 tcagtatcag cattgtgctc tatgagttta tcccacagtg cataaaaagg ctcttcccat
      421 tcctcacctg caggcaaagg gcaaccgggc ccgcctgacg aaatataaat atcgaaactc
      481 aggtcaggca cttcattttt ttgtcttaca tcaaaaaagc tacacactgt aaatttgctg
      541 aatcgctcaa gaatagagcg gatgctgcgc atgccttcat tgggttcacc attgtataag
      601 tcgagtactg caacgcgcac aggaataaaa ttcgtcattt ctttttttcc ttttttacgg
      661 gaatatcaat atttgtttca ctaattatgt aatctaccac ttttttaagg tctccggttt
      721 tctcaaaaac tgccaactgc ctgtcggcac cggtgcctct tttcatcata ctaaaaacat
      781 gatctacctc tttgcgcgag cctaaatcgc caacaacatc atcaataaac tctgtaattt
      841 cttcaatcag tagctttgtt tctacctcct tctctttacc aaaatcaatc agcttatcat
      901 caataccata acgggctgca cgccatttat tttcattgat taatgctctg gaataggttc
      961 tgaaactcag gttaaactgc atcagcaaat acaatttggc aataattccc tgcataatag
     1021 ctgccagcgt aatagtttct tcaatgcgca ttggaatatc acaaatcctg aattcaatag
     1081 tattgaaaaa cggatgcagt cttatatccc accaaacctt cttagcattg tccatgcagt
     1141 tggtttttac aagcaattga atgtaggcat catattcggc agcactcgaa aactcatcgg
     1201 gaatacccgt tctcggaaat ttatcaaaca ccttagttct aaaagactta aaacctgtgt
     1261 ttcttccttc ccaaaaagga gagttggtag agagtgcaaa aatgtgaggc aaaaaataac
     1321 gtgcctgatt catcagcctt atacctattt ctctgtcgtc aatgccaaca tgcacatgca
     1381 aaccaaaaat cagattgctt cgtgcagcat cctgcatttc gcgtataatt tcttcgtagc
     1441 gtggatggtc tgtaatcaac tgatcttgcc agcgcgaaaa cggatgtgta cctgctgcgc
     1501 caataaccaa tccttgtttt tgtgccagtt tagccaccat ctgacgcaga taggtaactt
     1561 ctttgcgggc ttccgaaaca tctttgcaaa tgttggtacc cacttccact acactctgat
     1621 gcatttcggc ctttacctgc tccttcaaaa tcactttgcc gccttctaca atctgatgca
     1681 tgtgcgagcg cagctctctc gtcttagggt ctataacctg aaactcctct tctacaccaa
     1741 gtgtaaattg tggcagccct aattttttat taatgtatga catattcttt tttacgctgg
     1801 ttatttcacc gtgtaagact ccatcagtgc atccatattc tgcttgttca caatctgtcc
     1861 ttgtgcagca tgtttaacaa aagctcccca tgtcaggtta tcctgtcctt cgcggtgcga
     1921 aagtgctttt tcaatacaca tatcggcaac ggcttctatc acccaattaa aattctcatc
     1981 gccaacactg tggcggtcgg catcgggggc cggattgcag aaatctatag cataaggcac
     2041 accatcacga atggcaaact caaccgtatt gatgtcatag cccaatgcat tgcaaagctt
     2101 aaaagtatat tcttcaacaa catcaagaat acgctgctcg ggtgcaggcc cgtcaaccac
     2161 ataacgcagg tgatgcgggt tgcgtggctc ataacgcata atctttatcc gcttgccgcc
     2221 tatacaataa caacggaaat actgatcaaa cacaatctcc tcctgcagca acatcaccaa
     2281 ttgacctgtt tcttgatgct tgttataaaa gtccgcggca tcattgcact tatacacatt
     2341 tttccatccg ccaccggcaa aaggtttcat ataagccgga aaacctatgt acttaaaaat
     2401 attatcccaa tccaagggca tcttcagatt tctgaaagaa ttttcattcg tatcgggcgg
     2461 atgaacatta ctcggaagca gaaccgtctt tggcacaggc acaccaattt ttgtggcaag
     2521 gcagttatta aaaaacttct catcggcact gttccagaaa ggattgttca gcactgctgt
     2581 gccgcaaata gcagcattct tcaacatacc acgataaaaa ggcacatcat gcgatattct
     2641 gtcaacaatc actgcatagt ccataggcat tccctgcacc accttgtcaa cctgcaccga
     2701 ttcggccaca atgccactaa tatttttagc gttaatccgc tctacaactg ccggtggaaa
     2761 actattctcc tgtccgtaca aaatgcctat tttcttcatt gtataataaa tgtatgatat
     2821 ccgacaaaaa taagaaacag aaacaaatgg gcaacaagtt tagatggtta gaaagttaca
     2881 aggttttgaa agttgaagga tttggagtgt tggtgaatag attcctttaa taaactaaat
     2941 caactttcat taatagctta aaagttattg gatgctacta tgcaataggg ttttaattat
     3001 ttttgataaa tcttaaatta ttatctactt tagtaaatca taattattga aaataaaggt
     3061 ttaagttacg gaaataaaca atgaggataa tatttttaat aattgtaatt ctacatgggc
     3121 tgatccactt gcttggcttt gttaaaggta taggcctcaa agaagtaaaa gagttaaccc
     3181 ttcctatatc taaacctctg ggatttattt ggcttgcttc tgcgttcata tttttagctt
     3241 atggtatttc atttattgtc aactataaat attcctggct tataggattt cttgccattg
     3301 ctgtttcaca aatattaatt attcttattt ggaaagatgc taaatttgga actataccca
     3361 atgcacttat ttttattgtt tcattctttt catggggata ctatagcttt caaaaatgca
     3421 ttttagaaga aacgcagcaa ttacttagtc aaaacaagat tattgaaaac aaaataatga
     3481 ctgaaagtga aattcaattg ttgccttatc cggtaaaaaa ctggattcgt cattctggcg
     3541 cattaggaag accgttgatt agtataggaa aagttgttca atctgctcaa ttaaaattaa
     3601 accccggaca agagaattgg atgaatgcca cagctattca atatacaaca attgataaac
     3661 cggcttttat atgggccgta gatgtaaaaa tgaataacat agtaagtttt aaaggcagag
     3721 ataaatttga aaacggcaat ggagaaatgt tgataaaaat gaattcgtta attaacattg
     3781 taaatgagca aggcgaaaaa ttaagtgaag catctttaca gcgtttttta ggtgagatgg
     3841 tttggtttcc atccttagca ctaagtcctt acatttcttg ggaacaaatc aatgatacca
     3901 ccgctagagc aacaatgagt tacaaaggca cttcgggtag cggaacattc tattttaatg
     3961 acaaaagcga catcactaaa tttactgcct ttcgttataa agacaataat gctgttgcaa
     4021 aaaaacatga atggagtata agcatttctg attataaaac attcgaaggc gtaaaagttc
     4081 ctgcaattat gtcttcgaca tggaaacttg atgcaggtga ttggacttgg ttaaaattag
     4141 aagtattgga tataaaatac aatgatgaga gttccaaata aacatttttt tgtcattcat
     4201 tacgtgaatg ttaatttaat tataactttt gacgtaatca atgttcatag caaagctgac
     4261 ttttaggttt catggatttc atttctaaaa agtaatatcc gctgcgatgg aaaaaaacta
     4321 ccaatatcaa aatatcgtcc gttccagaat tgcaactttt gacatattcg atattggctt
     4381 acggaagcat catatcaatg caattctgga attcgatgta acagaaagca gagcacaact
     4441 tcatgagctt agaaaaaatg ggactgtaat ttcttttaat gcatggctta tcaaagtaat
     4501 tggcactgtc ctgaaaaagc atcctgaagc agcatcatat atctatagta gaaaaagact
     4561 cattatgttc aatgacatta atgtttcatt gattgtagaa aaagaaattg ggaattcaaa
     4621 agttcctatt ccaattgtta ttgaaaaaac aaatgaaaaa tctgcctacg atattactat
     4681 cgaaattgaa aatgctaaaa gtcaaaaatt agatatagat aatattgtct tgaaaagtcg
     4741 agcaaataca ttccaaactc tatattacca cttaccaggt ttctttagaa aacttttctg
     4801 gagaatatta ctaagaaatc ctaaacttgc tttcaaaaca atgggaaatg tcgcagtaac
     4861 ctctattgga atgataggca gaattaatgg ttggtttatt cacaaatcag ttcatccaat
     4921 ttcttttgga atagcatcaa tcatcaaaaa acctatcgta attgaaaata aaatagagat
     4981 tagagaaata ttaaatatga ccattttggt tgatcatgat ataattgatg gcgcacctat
     5041 ggttcggcta ttaaacgatt tgacaaatta tattgaaacc gggaaagaaa taaatgctta
     5101 gtttatttca cctaccaggt cgcaccaccc ctgacaatgt gttttaacac aaacaattaa
     5161 ttgtaataca acatcgttcg ctgctaacgc cttcttaaat catttttctt tttacttttg
     5221 agctaatgat tagttttaat tccgatagtt atcagaaaga ctgatgttag cagaaagcag
     5281 ttccctacat tatatctaaa tttcactata tttgaaaagt gaatacaatt gaactatata
     5341 ggtcttctaa tggtgaaact caaattgaag ttcggtttga agatgaaact gtttggctat
     5401 cattaaacca aattgctgaa ctttttggtc gtgataaatc cgtaatttct agacatttaa
     5461 aaaatattta caaggaatct gaacttaatt atagttcaac tgctgcaaaa aatgcaacag
     5521 ttcaaactga aggaaaacga aatataaccc gagaaataga ttattataat ttagatgcaa
     5581 tcatctctgt tggctatcgt gtaaattcaa aacaaggaac tcaattcaga atttgggcaa
     5641 caaatcgatt aaaggaatac ttaattaaag gatattctat caaccaaaaa aggctgcaag
     5701 aactcaatca gattgttcaa tttattgctc aatccgaaaa taataccaat aaaatttcag
     5761 agacaaaagg acttttgaat atcctaacaa aatatgcaaa aagttttata ttactcaatc
     5821 aattcgacag caatgcaatt gagttgaaaa atcttgatga aaatataaca tacgagattg
     5881 attataacga agccgttatt tcaatcaatg agctgaaaaa tcaattaatt gagcaaaagg
     5941 aagctacaga actatttgga aatcaaaagg atcaaaattt tgaaggaatt t
//
LOCUS       DAOOOI010000111         5922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2561_length_5922_cov_8.556843, whole genome shotgun sequence.
ACCESSION   DAOOOI010000111 DAOOOI010000000
VERSION     DAOOOI010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2561_length_5922_cov_8.556843"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..>1715
                     /locus_tag="PKX59_09995"
     CDS             151..>1715
                     /locus_tag="PKX59_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91238.1"
                     /translation="MSRISYLILMLFFVNVSVNAAVTVSPATSGNCLNVTPSAFLPIG
                     NIVITEGANGDFAIQSNKTLILSAPTGFMFQPGSGNVSFLASRNITTASIAVTLTTIT
                     VTISVSGIPKADELIISNIYARASTSNVSGNILRKSPGGGTATIAGDVAGAGINHGTL
                     SSAGTGQIVTSVANGAWSNPATWQGGKIPLCTDNVIIYHQINVDVVTPSLASLIIKSG
                     AKLTANLPVTVNSNLTIEANAWYVHNNSSNAATTIFNGTEAIDDNATIEVNNWSAATV
                     PFMSGVSSTVGNLILNYNAVWQQDGLFAPSKIKGNVSVTNGQVVMDDGTGMSTLLNLN
                     HVSIYKTGAIIFAQGANRNLTLNTLSFTDSSKSNAITALMFRTYGTLLWNNAGSVYLS
                     HDFSAVEGTTGYNAFNATVSIGGDLNVVKGKVDFFRYVDGNGVIQVAGNTYCNLTAGN
                     WFRLADYNTINLDFTTNNFSISGTESCYLQGSDGSTNFNINGSFSYTASSLFVFSNNF
                     TNNALQRIDVLKD"
     assembly_gap    1716..1761
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1762..4484
                     /locus_tag="PKX59_10000"
     CDS             <1762..4484
                     /locus_tag="PKX59_10000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91239.1"
                     /translation="GNVGLAFTSGKLNTHISGNLNVSGSSTQLVSQFYPYGTDSVKFV
                     IDGNCSLNGGMFNVSWNRGPVSFISSGIFNQVNSNFGVVFHPFPGNYAACSVFIDSLN
                     FSGGTFKVFDSYITDGKTVSITIGSDVNINFQNSSDRFVLISNTGWWNNPELNLVING
                     DLKISGNSSAIFASNVGRGEENIVINGDFEIQGGNNAIGGSTSLGSQQHDISVLINGN
                     MTQTGGQFYYSVNEGNVDYNIQNNLIQTGGTQNLTYQDAAAKLNIGGQYLQSGGTFNF
                     HASTGNTSLSDSVFVQGNFSQSNGVLRFDNAQGSSNISQNVLILNSDSILLSGNGIIT
                     HANHLTTNTVFGKILFNKNGISTFQRATSTHDLQQVEMVVGSLTTLDVKSSIQPLLIA
                     SHVSSAPAVNNVFTINGVLDMGLQRIMARQQGNYYSQLSVGATGKIKTMNPNGFYSTS
                     AASTINTLIAGNNRMNFWLDAQSIIEYNGTQNQNITGIPNGIVNAVSNKYGKLQINFA
                     GVNNINFVKPEADSVVFVRSGLMLTSGELNLNADHIANTNGLSIVLENGCQILHASGY
                     IRSETIDGSARIHLSSQQSVNYEIPFGFNSTTYIPFKYQCSSGCDWVSLATYRSLPDN
                     TPFPPGVTHVRSISGADNSLQTIDRYWYVRAVGNPNANLRFTYAPIEKGVISTPRAQR
                     WIGNDAWESFKGSQSNPSPFETDVNGITGLNSWWTLSSQSSPLPVEILSFGANCQSQS
                     VILKWSVATQLNNDIFILERSRDGKKWENLTSIKGAGTTNTLMNYETKDEAPYSEVTY
                     YRLAQIDYDGKRNELKTIAVKGCGSDPIRILYYSFSDHKTVIKIQSSYNDKIEIFLFN
                     ENGQTVARKVQNISNNIQEIEMDCPQLNTGIYMLSIRGSFNNIIKKLVKN"
     gene            4496..4990
                     /locus_tag="PKX59_10005"
     CDS             4496..4990
                     /locus_tag="PKX59_10005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ91240.1"
                     /translation="MAEETLNMTATTFENLSQPNAAMDVFPINYSEKHIAELIHEIRR
                     RVFVLEQHIDPMIEFDTFEHESQHYLAYVKNIPAGTARWRHTDEGIKLERFSVLKEHR
                     NSGLGRMLVNRLLNDVKPIGKKVYLHAQTTTVRFYEKAGFKVVSELFYEANIPHFKME
                     LVIS"
     gene            complement(5016..5456)
                     /locus_tag="PKX59_10010"
     CDS             complement(5016..5456)
                     /locus_tag="PKX59_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91241.1"
                     /translation="MKQNVFLLCVILNITITGCQIFKINKSDKHGNRQGHWEHYWNDE
                     GTIQRKGKYKHGLEVKKWKYYDLNGKLIKQEKYSKKRDVINIKTYYDNKKIKSNGKAF
                     LINENDSILHFYWQGPWNYYSPTGVLEKTEWYEKGKIKKAVTYP"
     gene            complement(5453..>5922)
                     /locus_tag="PKX59_10015"
     CDS             complement(5453..>5922)
                     /locus_tag="PKX59_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOZ91242.1"
                     /translation="DYTANDSVLQFRKTELQDNVIPCANSVMANNLFLLSKYLNNENY
                     LLRSKKMLEKVIDQIPNYGFGYANWALLLQRINQPFYEVAFGGYNALKNKNKLNEYFL
                     PMKITAPIILKDKIEFTADKNETDDLIYVCRNHSCLLPTSDIIKVAQTISDQQ"
BASE COUNT         1917 a          955 c         1104 g         1900 t
ORIGIN      
        1 catccatgaa ggttatcatc aggttttatt agcttatctg ctgaaaagag aaaagttaca
       61 ttagttcata aattttcaga gtagttttta cagcattttt tcggaacatt ttttgaattt
      121 tatcgtagaa aacgggataa aactacagtt atgtcccgaa ttagttattt aatcctgatg
      181 ttgttttttg taaatgtcag tgttaatgcc gcagtaaccg tttcgccagc aacaagtggt
      241 aattgcctaa atgtcacacc atcggcattt cttcccattg gtaatattgt tattaccgaa
      301 ggtgccaatg gcgattttgc tattcagtcg aataaaacct taatcctaag tgcacctacc
      361 ggatttatgt ttcagcccgg atcaggaaat gtttcttttt tagcatcaag aaatattaca
      421 acagcatcaa ttgctgtaac attaacaacc ataacagtaa caatttctgt cagtggaatc
      481 ccaaaggctg acgaattaat aatttccaat atttatgctc gtgcatcaac aagtaatgta
      541 tccgggaata tactgcgtaa atcccctggg ggtggcacag ctacaatagc aggtgatgta
      601 gcaggcgcag gaattaatca cggtacattg tcaagtgccg gtacagggca gatagtaact
      661 tctgtagcaa atggagcatg gagcaatccg gcaacatggc aaggtggcaa gataccgtta
      721 tgtactgata atgtaataat ctatcatcag attaatgtag atgtagtgac accttcctta
      781 gcttcactaa taataaaatc aggagcaaaa ctcactgcta atttacctgt aacagttaac
      841 agtaatctca ccattgaggc aaatgcttgg tacgtacaca acaatagttc aaatgcagca
      901 acaacaatat ttaatggaac agaagctatt gatgacaatg ctaccataga agttaataac
      961 tggagtgcag ccactgtgcc ttttatgtcc ggtgtcagtt cgacagtcgg taatcttata
     1021 ttaaactata atgcagtatg gcaacaggat ggactgtttg ctccatcaaa aataaaggga
     1081 aatgtatcag taacgaatgg tcaggttgtg atggatgatg gtacaggtat gtcaacactt
     1141 ttaaatctta atcatgtaag catatataaa acaggtgcaa ttattttcgc acaaggtgct
     1201 aatagaaacc tgacattaaa tacactgtcg tttaccgata gcagtaaaag caatgcaata
     1261 acagcattga tgtttagaac gtatggtact ttgttgtgga ataatgcagg tagtgtttat
     1321 ctaagccatg attttagtgc agttgaaggt acaacaggct ataatgcatt taatgctact
     1381 gttagtattg gaggagattt gaatgttgta aaagggaaag ttgatttctt cagatatgtt
     1441 gatggtaatg gcgttattca ggttgcaggg aatacttatt gtaatctgac agccggtaac
     1501 tggtttcgac tagcagatta taatacaata aatcttgatt ttactaccaa taatttttca
     1561 ataagcggaa cagagagttg ttatcttcag ggttctgatg gctcaacaaa ttttaatatt
     1621 aatgggagtt ttagttatac agcaagcagc ttatttgttt tcagtaataa ttttaccaat
     1681 aatgcattac aacgaattga tgtgctaaaa gatttnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn natggaaatg tcggattggc tttcacaagt ggaaagttaa
     1801 atactcatat ttcaggaaat ttaaatgtaa gtgggtcgtc aacacaatta gtgtcgcagt
     1861 tttatcccta tggaacggac tcagtaaaat ttgtgataga tggtaattgt agcttaaacg
     1921 gaggcatgtt taatgtttca tggaatcgtg gcccggttag ttttatttca agcggaatat
     1981 ttaatcaagt gaattcaaat tttggagtcg tttttcatcc attccctggt aattatgcag
     2041 catgttctgt ttttattgac agccttaatt tttccggagg aacattcaaa gtttttgaca
     2101 gttatatcac cgatggaaaa acagtttcta taacaatagg atcagacgta aatattaatt
     2161 ttcaaaattc aagtgacaga tttgttctta ttagcaatac aggctggtgg aataatcctg
     2221 aactcaattt agttattaat ggtgatttaa aaatcagtgg aaacagcagt gcaatttttg
     2281 cgagtaatgt tggacgtgga gaagaaaata ttgtaataaa tggtgatttt gaaattcagg
     2341 gaggtaataa tgcaattggc ggctcaactt ctttaggatc acagcagcat gatatatcag
     2401 tattaattaa tggaaacatg actcagacgg gcggccagtt ttattattct gttaatgaag
     2461 gtaatgttga ttacaatatt caaaataatt taattcaaac tggaggcaca cagaatctga
     2521 cttatcagga tgcggctgca aagcttaata ttggaggaca atatttacaa tctggtggta
     2581 cttttaattt tcacgcaagc accggtaata catcactttc tgatagtgta tttgtacaag
     2641 gaaattttag tcagagcaac ggagttttgc gcttcgataa tgcacaaggg tcatcaaata
     2701 taagtcaaaa tgtattaatc ttaaactctg atagtatttt actttcagga aatggaatta
     2761 ttactcatgc caatcatctt acaacaaaca cggtatttgg aaaaattttg tttaataaga
     2821 atggaatttc cacatttcaa cgagcaactt caacacacga tttacaacag gtagaaatgg
     2881 ttgtagggtc tttaacaaca cttgatgtga aatcatccat tcaaccattg ctgatagctt
     2941 cacatgtttc atcagcaccg gcagtaaata atgtgtttac aattaatggt gtgcttgata
     3001 tgggattaca gagaattatg gcacgtcaac aaggaaatta ctattcgcaa ctttcagttg
     3061 gtgcaaccgg taaaattaaa acaatgaatc caaatggttt ttattcaact tctgcggcat
     3121 caacaataaa tacactcatt gcaggtaata acagaatgaa tttttggctc gatgctcaaa
     3181 gcattattga atataatgga acccaaaatc agaacatcac aggtattcca aatggtatag
     3241 tcaatgcagt ttcaaataaa tatggcaaac tacaaattaa ctttgccgga gtaaacaata
     3301 ttaacttcgt aaagcccgaa gccgacagtg tggtttttgt tcgctctgga ttaatgctta
     3361 cttccggtga attgaatctt aatgcggatc atatcgcaaa tacaaatgga ctttcaattg
     3421 tgttagaaaa tggctgtcag attttacatg cttcaggtta tatcagaagt gaaactattg
     3481 acggtagtgc aagaattcat ctttcatcac aacagagtgt taattatgaa attccatttg
     3541 gttttaacag cacaacttat ataccattca aatatcaatg ttcaagtgga tgtgattggg
     3601 tttcattggc aacatacagg agtttgcctg ataatacacc atttcctccg ggggtaacac
     3661 atgtccgttc aatttccggt gctgacaata gtttacaaac aattgatcgc tattggtatg
     3721 ttagagcggt tggaaatcct aatgctaatt tgagattcac ttatgcacct attgaaaaag
     3781 gggtaatatc aacacctcgt gctcagcgat ggataggtaa cgatgcatgg gaatcattta
     3841 aaggttcgca atccaatcca tcaccattcg aaacagacgt taacggaatt accggattaa
     3901 attcatggtg gacactaagc agtcagtcaa gtcctttgcc tgtagaaata ttaagttttg
     3961 gcgccaattg ccagagtcag agtgtgatat taaaatggtc tgttgccaca cagctcaata
     4021 atgatatctt tattttagag cgaagcagag atggaaaaaa atgggaaaac cttacatcca
     4081 ttaaaggtgc aggtacgacc aatacattaa tgaattatga aactaaggat gaagcaccat
     4141 attcggaggt tacctattac aggttagctc aaattgacta tgatggaaag agaaatgagc
     4201 tgaagacaat agcagtaaaa ggctgtggtt ccgacccgat aagaatatta tactattctt
     4261 tcagtgacca taaaacagtt ataaaaatac aatcttctta caatgataaa atagagattt
     4321 ttctatttaa tgaaaacgga caaacagtgg cacgaaaagt gcaaaacata agcaacaaca
     4381 tacaagaaat tgaaatggat tgtccacaac ttaatacagg aatttacatg ctaagcatca
     4441 ggggttcctt taataatata atcaaaaaat tagttaagaa ttaatttcta tttttatggc
     4501 cgaagaaact ttaaatatga ccgcaactac atttgaaaat ctctcacagc ccaatgccgc
     4561 aatggatgtg tttccgatta attattccga gaagcatatt gcagaactca tccatgagat
     4621 cagacgaagg gtgttcgttc ttgaacaaca tatagaccca atgattgagt tcgatacatt
     4681 tgagcatgaa agccaacatt atttggctta cgttaaaaat attcctgcag gtactgccag
     4741 atggcgtcat actgatgagg gaattaagct tgaaagattc agcgttctaa aagagcacag
     4801 aaattcaggt ttaggccgta tgttggttaa caggttgtta aacgatgtaa agccaatagg
     4861 taagaaagtt tacttgcatg cacaaactac cactgttaga ttctatgaaa aagcaggatt
     4921 taaagttgtt agcgagctat tctatgaagc taatattccg catttcaaaa tggagcttgt
     4981 aatttcatga catatattta gagttttaaa ggctgttatg ggtacgtgac agcctttttt
     5041 attttccctt tttcatacca ttctgttttt tctaacacac ctgtaggtga gtagtagttc
     5101 catggtccct gccagtaaaa atgtagtatg ctatcgtttt cattgattaa aaatgcctta
     5161 ccattagact ttattttttt gttgtcataa tatgttttta tattaatgac atctcttttc
     5221 ttactgtatt tttcttgttt tatcagtttg ccatttaaat cgtaatattt ccattttttt
     5281 acctctaagc cgtgtttgta tttccccttt cgttgaatgg tcccctcatc attccaataa
     5341 tgctcccaat gtccttgccg gtttccatgt ttgtctgatt tgttaatttt aaaaatctga
     5401 caccccgtta ttgtaatatt taaaattaca caaagtaaga atacgttttg cttcattgtt
     5461 ggtcagaaat agtttgcgca actttaataa tatctgatgt tggaagtaaa cacgagtggt
     5521 ttctgcatac ataaatcaag tcatcagttt catttttatc agcagtaaat tcaattttat
     5581 cttttagaat tattggtgca gtgattttca ttggtagaaa atactcatta agtttatttt
     5641 tattttttaa agcattgtaa ccaccaaaag ctacttcata gaatggttga tttattcttt
     5701 gtaatagtaa ggcccagttt gcataaccaa atccataatt tggaatttga tcaattactt
     5761 tttcaagcat tttttttgat cgcaacaaat aattttcatt gttgaggtat tttgaaagta
     5821 agaataaatt gttcgccatt actgaatttg cacagggaat aacattatcc tgaagctcag
     5881 tttttctgaa ttgcagaaca gaatcgtttg ctgtatagtc at
//
LOCUS       DAOOOI010000112         5661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2712_length_5661_cov_8.213343, whole genome shotgun sequence.
ACCESSION   DAOOOI010000112 DAOOOI010000000
VERSION     DAOOOI010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2712_length_5661_cov_8.213343"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    145..176
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<177..711)
                     /locus_tag="PKX59_10020"
     CDS             complement(<177..711)
                     /locus_tag="PKX59_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91243.1"
                     /translation="MEESVNEQTKADNREQNSRRALVFIVITILLGVNGLLLYQFFDK
                     KSHLEEVTRTLDNTSAERDALSAELQQVKAEFEKINQENSSLQAQLLSKDEEIKSKMA
                     QIRRMIESGDASQLKAAKAELERLRSMNQVYVAQLDSMRNANVALASQNESLNDMITV
                     EKNKSTALALENSTLTKR"
     gene            918..1601
                     /locus_tag="PKX59_10025"
     CDS             918..1601
                     /locus_tag="PKX59_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOZ91244.1"
                     /translation="MINDFFKLFFPDNCPGCGHSFLRGEKYVCLQCLYGLHKTQYHND
                     PENPIAKNFWGKTDFVAVTAVYFFQKGQVLQRLLHQLKYNGCKEIGIMLGRQMAMELF
                     QASAFRNADIIIPVPLHPGKLLQRGFNQSEMIALGMAEYWEKEVHTETLKRKKYTQSQ
                     THKARYERFENTESVFELDNIKSYEDKHVLLVDDVISTGSTIISCAEAFSGISGCKIS
                     MASVAAPHR"
     gene            1632..2138
                     /locus_tag="PKX59_10030"
     CDS             1632..2138
                     /locus_tag="PKX59_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOZ91245.1"
                     /translation="MSESIVIDTTLSKQEKYAQLLPQAEALIKGETDAVANAANLCAM
                     LKQAFGFFWVGFYFVKQHEGKQQLILGPFQGPVACTRIELGKGVCGTAWQQKETIVVP
                     DVNNFDGHIACSSLSRSEIVVPILKHDVVAVLDVDSEHLNHFDETDKYYLEKISSLYS
                     ETFSYATE"
     gene            2125..3744
                     /locus_tag="PKX59_10035"
     CDS             2125..3744
                     /locus_tag="PKX59_10035"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HOZ91246.1"
                     /translation="MLLNKINSFNRTFLLPAVLLLFGACASVVAPTGGKKDETPPKLK
                     EVIPPEQTVNFNSEKVVFTFDEYIQLLDKDKQLVISPIIEPAPTFDVKGKKLTITFKK
                     KLKENTTYTINFGNAVADLHESNPLAGFRYVFSTGTYVDTLFVKGNVVFAENLKSEKG
                     ILVMLYTSEDDSLPYNNPPAYFTRTDESGNFSIKNIARGPYKIFALKDADNNYKFNQS
                     DEWIAFDKTPITGGDSTPINLRMFKEAEKKVYVKEAKSFNAFAFSVLMNRPVGVLNIE
                     PLQTSDNIKLISRSKTNDTLMVWLKDSISDTLSYKLSGENNWTDTLTISKPAKALRGK
                     LKQSISLLSSTNIYPGNDVLLKTAIPVASVNKEKILLMHDSTTSQPFEIVFSDSASSN
                     ITIKSVTIEKNNWNLTLLPGAVTDIYGNKNDTAKLSFHTNAFTDYGSVKIKVDGLSGS
                     NYILQLVDEKDMVISEKYIGGNGSYLFEKMLPAKCRVRIINDADGNKSFTTGNYLLKQ
                     LPELVYYYKETLTLRANWDIEINWIIKEIKQ"
     gene            3741..4160
                     /locus_tag="PKX59_10040"
     CDS             3741..4160
                     /locus_tag="PKX59_10040"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOZ91247.1"
                     /translation="MTEQQRIDSGKAPEPVGLYPHARRVGNLLFLSGVGPREKGSKKI
                     PGVEQDASGNIVQYDIAAQCHAVFKNVRYILEDSGSSWDKIIDVTVFLTNMKDDFPVY
                     NKLWAEYFKENPPCRTTVEINCLPTPIAIELKVIATI"
     gene            4163..5158
                     /locus_tag="PKX59_10045"
     CDS             4163..5158
                     /locus_tag="PKX59_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOZ91248.1"
                     /translation="MYLITGASGLVGSQLVFDLISMGRPVRILLRKSSSTALIKKKFK
                     SSPELFDKIKITYGDVLDIFSIEDALQDCTHVFHCAAMVSFNPQKRKEMYKVNTEGTA
                     NVVNACLEKGNIKLGFVSSVATLGRGTQQMINENDWFNQSEKNSYYAISKYAAEREVW
                     RGITEGLDAVIINPSVILGDGNWRSDSSSLFNTLYSGLKFYTQGVSGFVDVKDVSRSL
                     ISLTESKISGERFIISSENKTYEWLFKTMAKGLNINPPTIKAGMFLSQLAWRLEWLKS
                     VFTGKPSLITNETAYTSQQIYNYDNSKITEQLNYRFIPIEETVERVCKLFIAQKQ"
     gene            complement(5139..5627)
                     /locus_tag="PKX59_10050"
     CDS             complement(5139..5627)
                     /locus_tag="PKX59_10050"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOZ91249.1"
                     /translation="MSGNLFFRYANYNKWANQRITNAIVSAGEVAADIEMKSSFNTIR
                     KTVYHIYDSQHTWIMRIQNKPYSWPPSKDFKGDLNEFSKLLIESSQEWINYISNLNDQ
                     DFKKSISYKNTKGIDHQTPLEEIIMHCFNHSTYHRGQLVTMLRQAGVNSFEPMDFIAF
                     VR"
BASE COUNT         1832 a          936 c         1076 g         1785 t
ORIGIN      
        1 agagcctgat gtggcaacag tgttttcaag aattgtaaag caggtcttga ttttttgtgc
       61 cgatgatgct ttgttggttt ctaactcctt gccactgctc ttatatttta ctccggatgc
      121 tttaattgaa gtagtcctta gtacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctct
      181 ttttgttaat gttgaatttt caagtgctaa tgcagtagat ttattttttt ctactgtaat
      241 catatcattc aacgactcat tttgtgaagc taatgcaaca ttggcatttc gcatggagtc
      301 taactgtgca acatatacct gattcattga tctcagtcgc tctaactctg cttttgcagc
      361 ctttaactgt gaagcatcac ccgactcaat catccttctg atttgcgcca ttttgctctt
      421 aatctcttca tccttactaa gcaactgagc ctgcaagctg gagttctcct gatttatttt
      481 ttcaaattcg gcttttacct gttgcaactc tgctgacaag gcatcgcgtt cagcagaagt
      541 attgtcaaga gttcttgtaa cttcttccaa atgacttttt ttatcaaaaa actgatatag
      601 caacaatccg ttaaccccca atagtatggt gattacaata aatacgagtg ctctgcgtga
      661 gttttgctca cggttatctg ctttggtttg ttcattcact gattcttcca tctatctgaa
      721 atgggttgtt aaacggtgca aaataatgta attagtattt acaagcaagt aaaaaggatg
      781 aaatattctc tgtttctacg taaaatgaca attttgagtt tccttggcca ccgcttaatt
      841 tgcttttttt attcggtaaa ccttacattt ttttaaacaa aagcatatat ttacttcttg
      901 atttaaaacc gccacttgtg ataaatgatt tttttaaatt gttcttcccc gacaactgcc
      961 cgggctgtgg gcatagtttt ttgagaggtg aaaaatatgt gtgtctgcaa tgtttgtacg
     1021 gacttcataa aacacagtat cataacgacc cggaaaatcc tatagcgaaa aatttctggg
     1081 gcaagacaga ttttgtagct gtcacggcag tttatttttt ccagaaaggt caggtgctgc
     1141 aacgactttt acatcagcta aagtacaatg gatgcaaaga aataggcatc atgttaggca
     1201 gacaaatggc tatggagttg tttcaggctt cagcattcag aaatgctgat attattattc
     1261 ctgttccact tcacccggga aaacttttgc aaagaggctt caatcaaagt gaaatgattg
     1321 ccctgggtat ggctgaatat tgggagaaag aagtgcacac agaaactttg aaacgaaaaa
     1381 agtacacaca gtcgcaaaca cataaagcac gctatgaaag atttgaaaat acagaaagtg
     1441 tatttgagct tgacaatatt aagtcttatg aagacaaaca tgttttattg gttgacgatg
     1501 taatctcaac aggctcaacc ataatttcat gtgcagaggc tttttcaggc attagcggat
     1561 gtaaaatatc tatggcttct gttgctgctc ctcatcgttg aatttctgat atttgcacag
     1621 aagtttttag catgtcagag tccatagtta tagatacaac actaagtaag caagaaaagt
     1681 atgcacagct tttgccgcag gcagaagcat tgattaaagg agagacagat gctgttgcca
     1741 atgctgccaa tttatgtgcc atgctcaagc aggcttttgg ttttttttgg gtaggatttt
     1801 attttgtgaa acagcatgaa ggaaaacaac agttgatatt aggtcctttt caggggcctg
     1861 tggcatgtac acgtattgaa ttgggcaaag gagtttgtgg tacagcatgg caacagaaag
     1921 aaacaattgt agtgcctgat gtaaataatt ttgatggaca cattgcctgc agctcactaa
     1981 gccgttcaga aatagtagtt cctatattga agcatgatgt tgttgctgta ttagacgtgg
     2041 acagcgaaca cctgaatcat tttgatgaaa cagataaata ttatcttgag aaaataagct
     2101 cactttattc agaaacattt tcctatgcta ctgaataaaa taaattcatt taatagaact
     2161 tttcttttgc ctgctgtact tcttttgttt ggtgcatgtg cttctgtcgt agctcccaca
     2221 ggtggtaaaa aagatgaaac gccaccaaaa cttaaagagg taattcctcc tgagcaaacc
     2281 gtaaatttta atagtgaaaa agtggttttt acttttgatg agtacattca gttgcttgac
     2341 aaagacaagc agttagtaat ttccccaatt atcgagcccg caccaacatt tgacgttaaa
     2401 ggtaaaaagc tgacaataac ctttaagaaa aagttgaaag aaaatactac ttatacaatt
     2461 aactttggta atgccgttgc cgatttgcat gagtcaaatc cattagcagg attcagatat
     2521 gttttttcta ccggaactta tgttgataca ctttttgtta aaggaaatgt agtgtttgca
     2581 gagaatctga aaagtgaaaa gggaatcctt gtaatgcttt acacatcaga agatgattcg
     2641 ctgccatata ataatccacc tgcctatttt accaggacag atgaaagtgg aaatttttca
     2701 ataaaaaata ttgcacgcgg cccatataaa atctttgcat taaaggatgc agataataat
     2761 tataaattca atcagtctga tgagtggatt gcctttgata aaactcctat aaccggaggc
     2821 gactcaacac ctataaattt gagaatgttt aaagaagctg agaagaaagt ttatgttaaa
     2881 gaagcaaaat catttaacgc ttttgcattt agtgttttga tgaacagacc ggttggagta
     2941 ttaaatattg aacctcttca aacttctgac aatatcaaac taattagccg cagtaaaact
     3001 aatgatacct tgatggtgtg gttaaaagat tctatcagcg atactttaag ttataaacta
     3061 tcaggtgaaa ataattggac cgatacatta acaatatcaa aaccggcaaa agcactcaga
     3121 ggcaaattaa aacaatcaat tagtttgctt tcaagtacaa atatttatcc gggaaatgat
     3181 gtgttactta aaacagctat tcctgttgca tcagtaaata aagaaaaaat tttgttgatg
     3241 cacgatagca ctacatcaca accttttgaa attgtttttt ctgattctgc atcatccaac
     3301 attacaatta aatctgttac tattgaaaag aataattgga atttaacttt actaccggga
     3361 gctgtaacag atatttatgg taacaaaaat gatactgcca aactaagttt tcataccaat
     3421 gcatttactg attacggaag tgtaaaaatt aaagtagatg gtttatcagg atcaaattat
     3481 atacttcaat tggttgacga aaaagacatg gtcatttcag aaaaatatat tggtggcaat
     3541 ggaagctatt tgtttgaaaa aatgcttcct gcaaaatgca gggttcgtat tataaatgat
     3601 gccgatggaa ataaatcttt tacaacaggt aattatcttt taaaacaatt gcctgagctt
     3661 gtatattact ataaagaaac actaacatta cgagctaact gggatattga aataaattgg
     3721 attattaaag aaataaaaca atgacagaac aacaacgaat tgattcaggg aaagcaccgg
     3781 agcctgtagg actttatcca catgcaagac gtgtaggaaa tttattgttt ttatcgggtg
     3841 tgggtccgcg agaaaagggt agcaaaaaaa ttccgggagt agagcaggat gcatcaggaa
     3901 atattgtgca gtatgatata gcagcacaat gtcatgcagt gtttaaaaat gtaagataca
     3961 ttctggaaga ctctggcagc agttgggata agattattga tgtgaccgtt tttctgacca
     4021 acatgaaaga tgattttccg gtttacaata aactatgggc agaatatttt aaggagaatc
     4081 caccatgtcg tacaacagtg gagataaatt gtctgccaac accaatagct attgagttga
     4141 aagttatagc tactatttga ttatgtatct tattacaggc gcttcaggct tggttggttc
     4201 acaacttgtc tttgatttaa tttcaatggg cagacctgtt agaatattgc ttcgaaaatc
     4261 atcttccaca gcgttaatca aaaaaaagtt caagtcatcg cccgagcttt ttgataaaat
     4321 taagattact tacggtgatg tgttggatat tttcagtatt gaagatgcac tgcaggattg
     4381 cacacatgtg tttcactgtg cagcaatggt gtcatttaat cctcaaaagc ggaaagagat
     4441 gtataaagtc aacactgaag gaacagccaa tgtggttaat gcctgtttgg aaaaaggaaa
     4501 tataaaattg ggatttgtaa gttccgtagc aaccttaggt cgtggcacac aacagatgat
     4561 caatgaaaac gactggttca atcagagtga aaaaaacagc tattatgcta taagtaaata
     4621 tgctgcagaa agagaagtgt ggcgtggcat aactgaagga ttagatgcag tcattattaa
     4681 tccgtctgtt atattgggtg atggtaactg gcggtcagat tcatcatcat tgtttaatac
     4741 cttgtacagt ggattgaagt tttatactca aggtgtttca ggttttgttg atgtgaaaga
     4801 cgttagccgc agtctgataa gtttgacaga aagcaaaata tcaggtgagc gttttataat
     4861 atcatcagaa aataaaactt acgagtggtt gtttaagaca atggcaaagg ggttaaatat
     4921 aaatccacca accataaaag caggcatgtt tctttcacaa ttagcctggc ggttagaatg
     4981 gcttaaatct gtttttacag gaaaaccgtc tttaatcacc aatgaaacgg catatacatc
     5041 acagcagatt tacaattacg ataacagtaa aataactgaa caactcaatt atcgttttat
     5101 tcccattgag gaaacagtag aacgggtttg taaattattt atcgcacaaa agcaataaaa
     5161 tccataggct caaaggagtt tactccggcc tgacgaagca tggtgaccaa ctgaccacga
     5221 tgataggtac tgtgattaaa acaatgcatg ataatttctt ccaacggtgt ttgatggtct
     5281 atacccttag tatttttata ggaaatgctt tttttgaaat cctgatcgtt cagattagaa
     5341 atatagttaa tccattcctg cgaactttca ataagcagtt ttgaaaactc atttaaatca
     5401 cctttaaaat ctttactcgg tggccaggaa taaggtttat tttgaatacg cataatccag
     5461 gtgtgttgcg aatcgtatat gtgatacaca gtttttctta tggtattgaa actgcttttc
     5521 atttctatat ctgcagcaac ttcccctgca cttactatgg cattggtaat tctttggtta
     5581 gcccatttat tataattggc atatcgaaaa aatagatttc cactcatgat ttttaaaata
     5641 aatttgaatc aaatataata t
//
LOCUS       DAOOOI010000113         5619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2736_length_5619_cov_24.022106, whole genome shotgun sequence.
ACCESSION   DAOOOI010000113 DAOOOI010000000
VERSION     DAOOOI010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2736_length_5619_cov_24.022106"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>5619
                     /locus_tag="PKX59_10055"
     CDS             <1..>5619
                     /locus_tag="PKX59_10055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HOZ91250.1"
                     /translation="VGVLGILKAGAGYVPLDPEYPQERLQYMIETAQMPLLITNKNLS
                     EKIPASKAEKILIEEIFEKHSSPLTQNITHSPTDIAYVLFTSGSTGMPKGVAMPHRPL
                     VNLITWQLKNTKVLGSTKTLQFAPISFDVSFQEIFSTLSCGGTLVMIEDEMRLNAVNL
                     LNFVAAEKINRLFLPFIALQHLCEVAENFEGDLSCLQEVITAGEQLQSTQFIKNFFKK
                     NKNCTLWNHYGPTEGHVVTAYQLSSNPDAWAALPSIGKPIDNAEIYILNEEGNVVADN
                     EDGELYIGGVSVAKGYLNRADLTQERFIENPYSNSQGKYFYKTGDLARYMPDGNIEYL
                     GRIDGQVKVRGYRIELGEIELTLDKHATVKQAVVIAREDEPGDKRLVAYCLPHDNKKI
                     LAGELRQFLQDKLPEYMMPSAFVQIEEFPRTPSGKIDRRSLPAPSNKRPDIGTVFVAA
                     ETDLQKTFVKTWSKLLKVAEIGINDNFFDLGGNSLLALKFIAELRQEHNLDLPVVKMY
                     QHPDIFSLTKFLTNGSATKSFYENAQDRFSSNIKGKIETVEDGIAVIGMAGRFPGANN
                     VDDFWKNIVEGKETVSFFKKQELDNDVPEEDKNDENYVAVRGIIEDADKFDAPFFNMN
                     PRLAEVTDPQQRIFLQVAWEAIENAGYAPDKYDGLIGVFAGMGNNTYFPNNVFHNKEA
                     INRVGSFQTMTANEKDYIATRIAYEFNTKGPAVSVHTACSTSLTAVTLAYESLLNHEC
                     DMALAGGVAITVPVNSGHIYNEGGMFSKDGHTRAFDAGANGTVFSDGAGAVILKRYKD
                     AIKDGDEIYAVIRGAALNNDGSEKASFTAPSVEGQAAVIAMAQAKAGVSPDSISYIET
                     HGTATPLGDPIEIEALTQAFRSKTDKKQFCAVGSVKTNIGHLTPAAGVAGLIKTCLAL
                     KNKILPASLNYSKANAAIDFKSSPFYVNSTLKKWTTENGEPLRAGISSFGVGGTNAHV
                     VVEEAPQKAESGIARKKHLFLLSGRTKDVLDSQTKNLQSFLEKNPDANLADIAYTLQT
                     GRKYFNHRRIISGGDVQEIVSAIQQANPKKTAARELTTGNPEIIFMFPGQGSQYVNMG
                     KNLYDDEIVFKDAVDTCCNILQPHLGIDLRTILYPEDAEKEKAEKLLKETIYTQPSLF
                     TIGYALAKLWMSWGIQPKGFIGHSIGEFAGAHFAGVFSLEDALMLVANRGRMMQELPH
                     GSMLSVRKAAAEIEKEIDSSCSIAAINGPALCVVAGPAEKIEALQKSLESREITAKLL
                     VTSHAFHSPMMEPMLKPFLEKVKSVKLNKPAIPFVSTATSKWITDAEATNPEYWMNHV
                     RATVRFAEGIQTLWGDKPQRVLLECGPRNTAATLAKQQAKEPAKQTAISSLSDSADNY
                     AEWEQILFALGQLWMSGVAIDWQNFYALEKRNRIALPTYPFEKKRYWLDPINVIQNIT
                     QENIQPSVSGFEFPIITQPSVNTTIETTNNTTMNRKDLITAELKTILEESSGMELGND
                     SSATFLDLGLDSLFLTQAALTISKKYGVKVTFRQLNEEFSSLQSLSAHLDANLPADAM
                     PAAKLAPQQAPQQTNIVMQQPAMQTTGMNPMQMMMMQQMQMMQMMMQQMNQPQQQVAP
                     SVQAHIEKAVEIKSHTKKEDVSENEKAELAKPFGAIARIEKGTSNDISSEQEKWLKQF
                     IAAYTEKTKASKKYCQDYRSVLADPRVVTGFKPALKEIIYQVVVNKSKGVNFHDIDGN
                     EYVDVLNGFGSNFFGWGNDMLKNAWKKQLDDGVELGPQTPLAGECAQLICELTGYDRA
                     GFCNTGSEAVLGAMRIARTVTGRNLIICFNGSYHGINDEVIVRGTKKLKSFPAAAGIM
                     PESVQNMLVLDYGTEESLQIIRERMDEIAA"
BASE COUNT         1892 a         1016 c         1169 g         1542 t
ORIGIN      
        1 ttgttggtgt attggggatt ctgaaggccg gtgccggata tgttcctctc gaccctgaat
       61 atcctcaaga gcgattgcaa tatatgattg agactgcgca aatgccactg ctcatcacca
      121 ataaaaatct ttcggaaaaa atacctgcaa gtaaggcaga aaaaatttta atcgaagaga
      181 tttttgaaaa acattcttcg ccactcactc aaaatataac gcactctcct actgacattg
      241 cctatgtact ttttaccagt ggatcaaccg gtatgcctaa gggtgttgcc atgcctcaca
      301 gacctttggt taatctgatt acatggcaat tgaagaatac caaggttttg ggctcaacaa
      361 aaacattaca atttgctccc ataagttttg atgtttcatt tcaggaaatt ttttctacac
      421 taagttgtgg tggcaccttg gtgatgattg aagatgaaat gcgattgaat gcagtaaatc
      481 ttttaaactt tgttgctgcc gaaaaaatta atcgtttgtt tttacctttc attgctttgc
      541 agcatctatg cgaagtagct gaaaattttg aaggcgattt aagctgtctg caagaagtta
      601 ttacagcagg agaacaatta cagtcaacac agtttatcaa aaactttttt aagaagaaca
      661 aaaattgcac attgtggaat cattacggac caactgaagg gcatgttgtt actgcatatc
      721 agctctcttc aaatcctgat gcttgggctg cactgccttc aattggtaag cctatagata
      781 atgcagaaat ttatattctg aacgaagaag gaaatgttgt tgcagataat gaagatggtg
      841 aactttatat tggtggtgta agtgttgcaa aaggctatct taatcgagct gacttaacac
      901 aggaacgatt tatagaaaat ccatatagca attcgcaagg aaaatatttc tacaaaaccg
      961 gtgatcttgc tcgttatatg cccgatggca atatagaata cctcggaaga attgatggtc
     1021 aggtgaaagt gcgtggctac agaattgaat tgggagaaat tgaattaact cttgataaac
     1081 atgcaacagt aaaacaagct gttgtaattg cacgagaaga tgaaccgggt gataaacgct
     1141 tagttgcata ctgtctccca catgacaata agaaaatttt agcaggagaa cttcgtcaat
     1201 tcctgcaaga taaattacct gaatacatga tgccatctgc atttgtacag atagaagaat
     1261 ttccacgcac accttcagga aaaattgacc gcagaagttt acctgcacca tctaataaaa
     1321 ggccagatat aggcactgta tttgttgctg cagaaacaga tttgcaaaaa acttttgtca
     1381 agacatggag taagttatta aaggttgctg agattggtat taatgacaac ttttttgatc
     1441 ttggtggaaa ctcacttctt gcattaaaat ttatagcaga actaaggcag gagcataatt
     1501 tagatcttcc tgttgttaaa atgtatcagc atcctgatat tttttctctg acaaaatttt
     1561 taactaacgg gtctgcaaca aaatcatttt atgaaaatgc acaggacaga ttctcttcaa
     1621 acattaaagg caagattgaa actgtagaag atggcattgc tgtaattggt atggcagggc
     1681 gttttcccgg tgcaaacaat gttgatgatt tctggaaaaa cattgttgaa ggcaaagaga
     1741 ctgtatcttt tttcaaaaaa caggaattag ataatgatgt gccggaagaa gataaaaacg
     1801 atgaaaatta cgtggctgtt cgaggtataa ttgaggatgc cgacaaattt gatgctccgt
     1861 tttttaatat gaatccgcga cttgcagaag ttacagatcc gcaacagcgc atttttttac
     1921 aggtggcatg ggaagctatt gaaaatgcag ggtatgctcc tgacaaatat gatggactaa
     1981 taggagtttt tgccggaatg ggaaacaaca cctattttcc gaataatgtt tttcataata
     2041 aagaagcaat taaccgtgta ggctcatttc aaacaatgac tgcaaatgaa aaagattata
     2101 ttgctacaag aatagcttat gagtttaata caaaaggacc ggctgtgagt gtgcatactg
     2161 catgttcaac atcgctcact gcagttacac ttgcctatga aagtctgtta aaccatgaat
     2221 gtgatatggc attggccgga ggtgtagcta ttacagtccc tgtaaacagt ggacatatct
     2281 ataatgaagg tgggatgttc agtaaagacg gacatacaag agcttttgat gcaggtgcca
     2341 acggaactgt attcagtgat ggtgcaggtg ccgttatcct gaaaagatat aaagatgcca
     2401 taaaagacgg tgacgagatt tacgcagtaa ttcgtggtgc tgccttaaat aatgatggca
     2461 gtgaaaaagc aagttttact gcacccagtg ttgaaggaca agctgcggta attgcaatgg
     2521 cacaggcaaa agcaggtgtg tcgcctgact ccatttcata tattgaaaca catggtactg
     2581 ccacaccact tggtgaccca atagaaattg aagcactcac tcaggcattt agaagcaaaa
     2641 ctgataaaaa acaattttgt gccgttggct cagtaaaaac caatatcgga cacctgacac
     2701 cggcagcagg cgttgcagga ttaattaaaa catgtcttgc actaaaaaat aaaatactac
     2761 ctgcttcact gaattattca aaagcaaatg ctgcaataga ttttaaatct tctccattct
     2821 atgttaattc caccctaaaa aaatggacaa cagaaaatgg tgagcctttg cgtgcaggca
     2881 tcagttcttt tggtgttggc ggaactaacg ctcatgtagt agttgaagaa gcacctcaaa
     2941 aagctgaatc aggcattgcc cgaaaaaaac atttgttttt actttcagga agaactaagg
     3001 atgtattgga ttcacaaaca aaaaatctac aatcgttttt agagaaaaat ccggatgcaa
     3061 atcttgcaga tattgcttac actttacaaa caggacgaaa atattttaat catcgcagaa
     3121 ttatttcagg tggtgatgtt caagagattg tatcagcaat acaacaagct aatccaaaga
     3181 aaacagctgc acgcgaactg acaacaggca atcctgaaat aatttttatg tttcccggac
     3241 aaggttcaca gtatgtaaac atgggaaaaa atttatatga tgatgaaata gtttttaaag
     3301 atgccgttga cacatgctgt aacattttgc aaccacattt aggtattgac ttgcggacaa
     3361 ttctttatcc tgaagatgct gaaaaagaaa aagcagaaaa gcttttgaaa gaaactattt
     3421 atacgcagcc atcgttgttt acaatcggct atgcattagc aaaactttgg atgagttggg
     3481 gtattcaacc caagggattt atcggtcaca gcattggaga atttgcaggt gcacattttg
     3541 caggagtatt ttctttagaa gatgcactga tgcttgttgc caatcgcgga cgaatgatgc
     3601 aggaattacc acatggaagt atgttaagtg ttcgtaaggc tgctgcggaa attgaaaagg
     3661 agattgattc ttcttgttct attgctgcaa ttaacggccc tgcactttgc gtagttgccg
     3721 gccctgctga aaaaattgaa gcacttcaaa aatcattaga aagtcgcgaa attacagcaa
     3781 agttattagt gacatcacat gcattccatt cgccaatgat ggagcccatg ttgaaacctt
     3841 ttttagagaa agtaaaatcc gttaaactca ataaacctgc aataccattt gtatcaacag
     3901 ccacctcaaa atggattaca gatgcagaag caaccaatcc ggaatactgg atgaatcacg
     3961 taagagctac tgtacgtttt gctgaaggta ttcaaacttt gtggggtgat aaaccacaac
     4021 gtgttttgtt ggaatgtgga ccaagaaata ctgctgcaac cttagctaaa cagcaggcaa
     4081 aagaacctgc caaacaaaca gcaatttcat cgctgagtga ttcagcagat aattatgcag
     4141 aatgggagca aatactcttt gccttaggtc aactgtggat gagcggagtt gcaattgact
     4201 ggcaaaattt ttatgcactt gaaaaacgaa atcgcattgc actacctact tacccttttg
     4261 aaaaaaagag atattggctt gacccaatta atgtcattca aaatattaca caagaaaaca
     4321 ttcaaccatc agtttccggc tttgagtttc caatcattac tcagccatct gtaaacacaa
     4381 caattgaaac aacgaacaat acaacaatga atagaaaaga tcttatcaca gcagagttaa
     4441 aaacaattct tgaagaatct tcaggtatgg aattgggcaa cgacagcagt gcaaccttcc
     4501 ttgatttagg attggattca ctttttctga cacaggctgc cctgaccatc agcaaaaaat
     4561 atggagtaaa agttacattc agacaactta acgaagagtt ttcatcactg caatcgcttt
     4621 cagcgcattt agatgctaac ctaccagcag atgcaatgcc tgcagctaaa ttggcaccgc
     4681 agcaagcacc gcagcaaaca aatattgtca tgcaacaacc ggcaatgcaa acaacaggca
     4741 tgaaccccat gcagatgatg atgatgcagc aaatgcaaat gatgcagatg atgatgcaac
     4801 agatgaatca gccacaacaa caagttgcac cttctgttca ggcgcatata gagaaagcgg
     4861 ttgagataaa atcgcacact aagaaagaag atgtaagcga aaacgaaaaa gctgaattgg
     4921 caaaaccatt tggcgctatt gccagaattg aaaaaggtac atcaaatgac atatcatctg
     4981 agcaggaaaa atggttgaaa caatttatag cagcatacac tgaaaaaaca aaagcttcaa
     5041 aaaaatattg tcaggattat cgcagtgtgc ttgccgaccc acgtgtggtt acaggattta
     5101 aacctgcttt gaaagaaata atttatcagg ttgtagttaa caaatctaaa ggagtaaact
     5161 ttcacgatat agacggcaat gaatatgtag atgtgttgaa tggttttggc tccaactttt
     5221 ttggttgggg aaacgacatg ctaaaaaatg catggaaaaa acaattggat gatggcgttg
     5281 agcttggtcc acagacaccg cttgcaggcg agtgtgcaca attaatttgt gaactcaccg
     5341 gatatgaccg tgcaggtttt tgcaacacag gcagcgaggc tgtactcggt gctatgagaa
     5401 tagcacgtac cgttactgga agaaatctta ttatttgttt caatggttct tatcatggca
     5461 tcaatgatga agtgattgtt cgtggaacaa aaaaactaaa atcgtttccg gcagcagcgg
     5521 gaattatgcc ggagtctgta caaaatatgc ttgtattaga ttatggcaca gaagagagtt
     5581 tgcagattat ccgcgagcgc atggatgaaa ttgctgccg
//
LOCUS       DAOOOI010000114         5597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2749_length_5597_cov_29.337784, whole genome shotgun sequence.
ACCESSION   DAOOOI010000114 DAOOOI010000000
VERSION     DAOOOI010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2749_length_5597_cov_29.337784"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PKX59_10060"
     CDS             complement(<1..611)
                     /locus_tag="PKX59_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91251.1"
                     /translation="MKKLFTLIFFAIAGLVVADILNEQGQAVATGSPGEFDCTSCHTG
                     NNVNSGTGSISISSPNMPNWQYVPGQVYQIDVTVAQTGAPLFGFGFEALRTSNNTNGG
                     TFQITNTSSTFLKTGFINGSNRTNVVHKKNGGLSNDSHTFSFNWTAPATNIGNIIFYS
                     AGNAANNQGDTLGDFIYKTQQLITPSITGINEIQSSAISVYPNP"
     gene            complement(1000..2616)
                     /gene="pckA"
                     /locus_tag="PKX59_10065"
     CDS             complement(1000..2616)
                     /gene="pckA"
                     /locus_tag="PKX59_10065"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOZ91252.1"
                     /translation="MNEYGLRNPNFSVAELGLKNVAAAYWNLTVAELVEEVVIRGEGE
                     LTDFGALAVDTGEFTGRSPKDKFIVADKETENSVWWGDINIKFDADKFDALYNRVTAY
                     MSGKEIYVRDCYACADPRYRLNIRVITETPYQSIFSNNLFLRPTPEEITTMEKDWVIM
                     AASGFMADPKIDGTRQHNFAIVNFTKKIILIGGTGYTGEIKKGIFSVLNYILPHHKNV
                     LSMHCSANIGKNNDTAIFFGLSGTGKTTLSADPNRGLIGDDEHGWSDNTVFNFEGGCY
                     AKCVNLSKEKEPQIYQAIRFGALLENTEFFEGTTRVDFDNISKTENTRVAYPIHFIDN
                     AVEPSVAGIPKNIFFLTCDAFGVLPPISRLTTGQAMYHFISGYTAKVAGTEVGITEPT
                     TTFSACFGKAFLPLHPTKYAELLGKKLSENKDIRVWLVNTGWSGGSYGVGSRIKLAYT
                     RAIITAALNGELDNVKFETLPVFGLQYPVSCPNVPAEILNPKATWADKTSFDNKSADL
                     AAKFVKNFEQYASHANKEIMDAAPKVAVVA"
     gene            2794..4275
                     /gene="accC"
                     /locus_tag="PKX59_10070"
     CDS             2794..4275
                     /gene="accC"
                     /locus_tag="PKX59_10070"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOZ91253.1"
                     /translation="MFKKILIANRGEIALRVMRTASDMGIKTVAVYSDADRMAPHVLY
                     ADEAVHIGASPSAQSYLVVEKIIQAAKLTGAEAIHPGYGFLSENADAAQAIINAGLVF
                     IGPSPQSMRTMGSKLGAKEAAKKFNVPLVPGTDYAISDIDKARQIAVDIGFPVLIKAS
                     AGGGGKGMRIVESEKAFNESVKSAMSEAQSAFGDGSVFIEKYIASPRHIEIQVMGDNH
                     GNVVHLFERECSVQRRHQKVIEEAPSGVLDEKTRSAMGQCAVMVAKACDYSGAGTVEF
                     LLDEKNNFYFLEMNTRLQVEHPVTEMITGIDLVREQLNVAAGNKLSFSQDDLKIHGHA
                     VEVRVYAEDPANNFLPSVGKLTTYVPPRGVGVRVDDGYEQGMEIPIYYDPMIAKLVTY
                     GRDRNEAIARMIRAIDEYKITGCETTLPFCRFVMEHKAFVTAKFDTHFVKKYFTPVVL
                     EKTIADEAEIASLAAQLWLDEKKPEGIQQQQEKSISYWRNNRS"
     gene            4296..4943
                     /locus_tag="PKX59_10075"
     CDS             4296..4943
                     /locus_tag="PKX59_10075"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOZ91254.1"
                     /translation="MIKKILNCKIGNAFAKTSVMKFSVWLCFLLTAFIKFSIGQTPVG
                     SSTAIYDGDTIAYYTLNAVDIYGFIPANASDDVRKYMKLRRDVLKAYPYAKIASAELR
                     FIEESVSKMTSEKEKKKFIKEQEKIMKEQFEKDLKNLTYTQGKILIKLIDRETGHTSY
                     NLVKDLRGSLQAFFWQGIAKIFSANLKSEYDPDGADKMIEKVVQSIEKGELQVRN"
     gene            5006..>5597
                     /locus_tag="PKX59_10080"
     CDS             5006..>5597
                     /locus_tag="PKX59_10080"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOZ91255.1"
                     /translation="MALASKSNLQEGLTYDDVLLVPAFSEILPRDVDTSTSFSRNIKL
                     NVPIVSAAMDTVTESAMAIAIAQEGGIGVLHKNMSIEQQTNEVRKVKRSESGMIHDPI
                     TLHQNSTIHEALGIMREQRIGGIPIVNDSKKLVGILTNRDLRFETDESRKVKEVMTSG
                     QLITVTGVVSLQKAEVILRQYKIEKLPVVDRSGKLIG"
BASE COUNT         1813 a         1008 c         1110 g         1666 t
ORIGIN      
        1 ggattaggat aaacagaaat ggcagatgat tgaatttcat ttattcctgt aatagaaggc
       61 gtaataagtt gttgtgtttt gtaaataaaa tcacctaatg tatctccttg gttgtttgca
      121 gcatttccag ctgaatagaa aataatattt ccaatgttgg ttgcaggagc tgtccagtta
      181 aatgaaaatg tatgcgaatc atttgagagt ccgccatttt tcttatggac aacattggtt
      241 ctgtttgaac catttataaa acccgtctta agaaatgtgc tggaagtgtt ggtaatctga
      301 aaagtacctc cgtttgtatt gttacttgtc cggagtgctt caaacccaaa tccaaacagc
      361 ggagcaccag tttgtgcaac tgtaacatca atttgataca cctggcctgg aacatattgc
      421 cagttaggca tatttggtga tgaaattgaa atggaaccgg taccgctatt tacattattg
      481 cctgtatgac atgatgtaca atcaaattca ccgggagaac ctgtggcaac agcctgtcct
      541 tgttcattta aaatatctgc aacaacaaga cctgcaattg caaagaaaat tagtgtaaag
      601 agtttcttca taacctgtat ttttatttta acaggtcaaa tatgatgaca atttgtaaaa
      661 atcaaaaaag caatagctct aaaaggaata atttccttcc gaaaagggga taaacttatc
      721 tgtactgttt aaaaactacg gcaacaaatt acttgctgac atgaaaatgg aaatggtggc
      781 ggttgctttt tgaacgcacc ttaatacaga aaaagaaaac gatggctgta atgttgagta
      841 atgtaaatag taataacatg cctctaaagt aatatcaaca tcgaagagaa tatatactat
      901 ttggtaaatg gaaagttttt ttaggcaaaa atcaattcag cataagtcaa aaaaaactcc
      961 cccgcgattt tcgcggggga gaaaaaaaat taaaaaaaat taagctacta cagccacctt
     1021 aggtgctgca tccataattt ctttattagc atgtgaagca tactgctcaa aattcttcac
     1081 aaacttagca gctaaatcag ctgatttatt gtcaaatgaa gttttatctg cccatgtagc
     1141 tttgggattc agaatttcag caggcacatt aggacatgaa acgggatatt gtaatccaaa
     1201 caccggtaag gtttcgaact taacattatc taattcacca tttaatgcag cagtaatgat
     1261 ggcgcgtgta tatgcaagtt taattcttga accaacacca taagaaccac cgctccatcc
     1321 cgtattcact aaccaaacac gaatgtcttt attttcactt agtttcttac caagtaattc
     1381 agcatatttc gttgggtgca ggggtagaaa tgctttacca aaacaagcag agaatgttgt
     1441 tgtaggttca gtaatgccaa cttctgttcc tgcaactttt gcagtatatc cactgatgaa
     1501 gtgatacatt gcctggcctg ttgtaagtct tgaaatagga ggcaatacac caaaggcatc
     1561 gcaagttaaa aagaagatgt tctttggaat accggccact gaaggttcaa cagcattatc
     1621 tataaaatgt attgggtagg ctacacgagt gttttctgtt tttgaaatat tgtcaaaatc
     1681 aacacgggtt gtaccttcaa agaattctgt attttctaag agtgcaccaa agcgtattgc
     1741 ctgataaatc tgtggttctt tttctttgct caggtttaca catttagcat aacatccacc
     1801 ttcaaaattg aatacagtat tgtcgctcca accatgttca tcatcaccaa ttaaacctct
     1861 gttagggtcg gcactcaatg ttgtttttcc tgtaccggaa agtccgaaga agatagcagt
     1921 atcattgttt ttaccaatgt ttgcagagca gtgcatactc aatacattct tatgatgtgg
     1981 cagaatgtaa ttcagtacag agaaaattcc tttttttatc tctcctgtat aacctgtacc
     2041 gccaataaga ataatctttt tggtaaaatt aacgatggca aaattatgct gacgcgttcc
     2101 atcaattttt ggatcggcca tgaaacctga tgctgccatg attacccaat ctttttccat
     2161 agtagtaatc tcttccggtg taggtcttaa aaataagttg ttagagaaaa tactttgata
     2221 tggcgtttcg gtaataacac gaatgtttaa acggtagcgt gggtctgcac aagcatagca
     2281 gtcacgtaca taaatttctt tgccggacat atatgctgta actctgttat aaagagcatc
     2341 aaatttatcg gcatcaaact taatattgat atcgccccac caaacagaat tttcagtttc
     2401 cttgtcggca acaataaact tatcttttgg agaacgtcct gtaaactcac cggtatctac
     2461 tgcaagggca ccaaagtctg taagttcacc ttcacctctg attacaactt cttcaacaag
     2521 ttctgcaact gtcagattcc aataggctgc tgctacgttc tttaaaccaa gttcagcaac
     2581 agaaaagttt gggtttctta atccgtattc attcataata tttcatcgtt tttatgaggt
     2641 gcaaagtaac aaaaaataaa gaaattcaag atgacttata gttacttttc tgtacagaac
     2701 ttattaacct gtattttgga aaaatttagc atttgtaaaa ggtttgattc acagttcgtt
     2761 ttacctttgc ccaataatgt aaaaagcgac ttcatgttta aaaaaatatt gattgccaac
     2821 agaggtgaaa ttgcattgcg ggttatgcgt actgcaagtg atatgggaat taaaacggtt
     2881 gctgtttata gcgatgccga ccgcatggca ccacacgttc tatatgcaga cgaagctgtg
     2941 cacataggtg cctcaccatc agcacaatct tacttagtag ttgaaaagat tattcaagct
     3001 gcaaaactta caggagcaga agctattcac cccggatatg gattcttatc agaaaatgcc
     3061 gatgctgccc aagctataat taatgccggt ttggttttca tcgggccttc accgcaatcc
     3121 atgcggacta tgggtagtaa attaggggca aaagaagctg ctaagaagtt taatgtgcct
     3181 ttagtacccg gaacagacta tgccattagt gatattgata aagccagaca aattgctgtt
     3241 gatataggtt ttcctgtatt gattaaagcc agtgccggtg gtggtggaaa aggaatgcgt
     3301 attgttgaga gtgaaaaagc ctttaatgaa tctgtaaaat cagccatgag cgaagcacaa
     3361 tctgcctttg gtgacggtag tgtttttatc gaaaaatata ttgcttcacc ccgacatatt
     3421 gaaattcagg taatgggcga taatcatgga aatgttgttc atctttttga aagagaatgt
     3481 tcggttcagc gcaggcacca gaaagtaatt gaagaagcac catcaggagt tttagacgaa
     3541 aaaacaagat cagccatggg ccaatgtgca gtaatggtgg ctaaagcatg tgactattca
     3601 ggagcaggta cggtagagtt tttgcttgat gaaaaaaata acttctactt tcttgaaatg
     3661 aatacacgct tgcaggtaga acatcccgta acagaaatga taacaggaat tgatttggtg
     3721 agagagcaat tgaatgttgc tgccggaaat aaattaagtt tcagtcagga tgatttaaag
     3781 atacatggtc atgccgttga agtcagggtt tatgcagaag atcctgccaa taactttctt
     3841 cccagtgtag gtaaactaac tacctatgtt ccacctagag gggtaggtgt aagggttgat
     3901 gatggttatg aacaaggaat ggaaattcca atttattacg acccgatgat tgccaagtta
     3961 gttacctatg ggcgcgacag gaatgaggca atagcaagaa tgattagagc aattgatgaa
     4021 tataaaatta caggctgtga aaccaccttg ccgttttgcc gctttgtgat ggagcataaa
     4081 gcttttgtta ctgcaaaatt tgatacgcat tttgtaaaga aatattttac accagttgta
     4141 cttgaaaaaa caatagcaga cgaagctgaa atagcttcct tggctgcaca actgtggctt
     4201 gatgaaaaga agcctgaggg tattcagcaa caacaggaaa aatctatttc ttactggcgc
     4261 aacaaccgat cctgaaccaa cgtttggtta aacaaatgat taagaaaatt ttgaattgta
     4321 aaatcggcaa tgcatttgct aaaacttctg tcatgaagtt ttcagtttgg ctttgcttcc
     4381 tgttgacggc ttttatcaaa ttcagcattg ggcaaacacc ggtgggttca tcaacagcaa
     4441 tttatgatgg cgatactatt gcctattaca ctttaaatgc agtagatatt tacggtttta
     4501 tccctgccaa tgcatctgat gatgtacgca aatacatgaa actgcgcaga gatgttttga
     4561 aagcatatcc ttatgctaaa atcgcttctg ctgaacttag atttattgaa gagtctgttt
     4621 ccaaaatgac ttcagaaaag gagaagaaaa aattcattaa agagcaagaa aaaatcatga
     4681 aagaacagtt tgaaaaagat ttaaaaaacc tgacttatac gcagggtaaa atccttatca
     4741 agttgattga tcgtgaaaca gggcacacct cttataatct cgttaaagat ttacgtggct
     4801 cacttcaggc atttttctgg cagggtatag ctaaaatttt cagcgcaaac ctcaagtctg
     4861 agtatgaccc tgatggcgca gataaaatga ttgagaaggt agttcagtct atagaaaaag
     4921 gcgagcttca ggttagaaat taacttttaa tttactccac tcaagcggtt ttattctgta
     4981 tctttgcccg gcaaaagcaa ataccatggc acttgcatca aaatcaaact tacaggaagg
     5041 attaacctac gatgacgttt tactcgttcc tgctttctcg gaaatactgc cccgcgatgt
     5101 tgatacttca acttctttct caagaaatat aaagctcaat gtacccattg tttcagcagc
     5161 aatggatacg gttaccgaat ctgcaatggc catcgcaatc gcacaggaag gcggcattgg
     5221 tgttctgcat aaaaacatgt ctattgaaca acaaaccaat gaagtaagga aagtaaaacg
     5281 ttctgaaagt ggtatgattc atgacccaat tactttacat cagaactcca ccatacacga
     5341 agctcttggc atcatgcgcg aacaacgtat tggaggtatt cctattgtaa atgacagtaa
     5401 gaagttggta ggcattttaa ctaacagaga tcttagattc gaaacagatg aaagccgtaa
     5461 ggttaaagaa gttatgactt caggacagtt gattactgta acaggagtgg tttctttaca
     5521 aaaagctgaa gtgattttgc gtcagtataa aatagaaaaa ttacctgttg ttgaccgttc
     5581 gggaaaatta ataggat
//
LOCUS       DAOOOI010000115         5547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2780_length_5547_cov_5.491624, whole genome shotgun sequence.
ACCESSION   DAOOOI010000115 DAOOOI010000000
VERSION     DAOOOI010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2780_length_5547_cov_5.491624"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(399..1571)
                     /locus_tag="PKX59_10085"
     CDS             complement(399..1571)
                     /locus_tag="PKX59_10085"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOZ91256.1"
                     /translation="MPEQKGRKYDISDANMLTDANNKRAYFIEDQADFTAFDPDFANP
                     YETDWETKIEAAETYIDDETVVDQMVGKTEYLHTTMKECRDYYQNAKHFIEKAFPNNV
                     SVQGEFGFNDYEKARQTQPKMVLFMYKFHAVAVQYSAELIAKNYTQAMIDGIELLADK
                     LKAANIQQDTFIGNRGVKTQTRSELHNAVWDVVTYVCKTGKLIYKNNYAKYNRYLLPS
                     SEEPDEELMLKGFVTDSLTNLKLDTVTVAIQGLGINTQTDSLGKYGFGLLPPGTYTLE
                     VSKPGYTTQIIPGIVVAENNTTTQDITLVPAATTGTVSGNVQQTAINIPNAFVSIDGF
                     PLLTTTTDINGNYTLNNVPAGMQVVRAQLPPINGGASQTQNVNVVAGNEVSASFMF"
     gene            complement(2099..2434)
                     /locus_tag="PKX59_10090"
     CDS             complement(2099..2434)
                     /locus_tag="PKX59_10090"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HOZ91257.1"
                     /translation="MKKLRVPIPTGYRFIPVANIVYIECINKITTIYLSGASKERIVS
                     IKSLLFFETVLKPFGFGRMNKNQVVNHACIIFQDKHKIPNLKLEGYEEVKIPMSLDYK
                     QKHGGFYIG"
     gene            2565..3089
                     /locus_tag="PKX59_10095"
     CDS             2565..3089
                     /locus_tag="PKX59_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91258.1"
                     /translation="MPNTKFRMMKKLLILLNTILVMFNCTAQESDTASVSTSIKIQKQ
                     QGGRTLYLQGNNELSKKELLYVLEDSPSAMEELRYYELARGVGFVTGAAGGFCIGWYW
                     GGYIMGKRYNPTLLGVGVAGALTSILCEFAARKNLHNAINIYNNETHKPTGSNTLQLN
                     FGFSEQGLGLNLKF"
     gene            3093..3725
                     /locus_tag="PKX59_10100"
     CDS             3093..3725
                     /locus_tag="PKX59_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91259.1"
                     /translation="MKKLLLLLVMLNLFQHLLLAQTIITYNTYYLIPPSSGCNGLWAV
                     GPYNPAECNTGIYSINPLGCATTSSTSGDTLFLTLCSIPCDFIAMDGTGGICLNCFLD
                     YDFSTTVTESATTAFTLYPNPASTTISILPIAIGTMVNSPWSPDSYREVNIYNTQGQL
                     VSHPLSRGECASLSAGKGCVLDISQLTPGLYYLHLQSAEGVGVKKFEVMR"
     gene            3761..4000
                     /locus_tag="PKX59_10105"
     CDS             3761..4000
                     /locus_tag="PKX59_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91260.1"
                     /translation="MNLQTEKARIKDELDGINDARLIKAIRELLNYAKQSSEERLLKP
                     FTKQQLVKRALASEKDIAAGRTTTLKQLRKEVKNW"
     gene            4005..4313
                     /locus_tag="PKX59_10110"
     CDS             4005..4313
                     /locus_tag="PKX59_10110"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOZ91261.1"
                     /translation="MRVELTDFAKSELRNIYTYYSEVSTDEIASKIIARILDDIELLE
                     NYPLAGANEQQLAELGREHKNIVSGNYKIIFRIHKTTVYVTDIFDCRQDPKKIPARNR
                     "
     gene            4317..4790
                     /locus_tag="PKX59_10115"
     CDS             4317..4790
                     /locus_tag="PKX59_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91262.1"
                     /translation="MQAHFPIRYGRNYKVVMAIVLPCLLIWPFIEALQLIKPLEEWLL
                     WVSIFGFLGIISAFSVWLATRVYPEATLSIHNNQVSLKFNSSNFLSPADFTFTIADIT
                     SFTRGEVGSDEYYIIITQNPRRKFQLSASTYKVEDMLLFNQAMVEISEKVRGVNS"
     gene            complement(4810..>5225)
                     /locus_tag="PKX59_10120"
     CDS             complement(4810..>5225)
                     /locus_tag="PKX59_10120"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOZ91263.1"
                     /translation="KDAGADILTVHIEASTHLHRTLQLIKAAGMQAGVAVNPHTAINQ
                     LEDIITDVDLVCLMSVNPGFGGQKFIPGTFQKLEKLRKLIDSTGSKARIEIDGGVGST
                     NAAQLINAGADVLVAGNFVFSSADPVGTIAGLKKL"
     assembly_gap    5226..5238
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5239..5452)
                     /locus_tag="PKX59_10125"
                     /pseudo
     CDS             complement(5239..5452)
                     /locus_tag="PKX59_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201866.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
BASE COUNT         1510 a         1243 c         1230 g         1551 t
ORIGIN      
        1 tatcattccg taggcaacat tgtcggcagt tacggttagg ttagagatat ccacatccac
       61 tcccgtaaga gcatgtgcta tctggctggc agtaggggtg ggtgtaataa ttacctgtgc
      121 ctgaatcaca acaggcagga gcagaaaaac gagtgctaat tttttcatga tgttacaaag
      181 ttgggttaat attttactct gtaacggaaa aattcaggaa ttattataca cctctaaaca
      241 aaaacatccc gcagtgcaga acctcaccct cggtccctct cctgcgagga gagggaagca
      301 gagtgctatt tatcagatgt caaaaaatat atgggcagtt tgtgtgctac gcggctgccg
      361 ttcccctctc ctgaaggaga ggggttaggg gtgaggtact aaaacataaa actcgcactt
      421 acctcatttc ctgctactac gtttacgttt tgtgtttgtg atgctccgcc atttataggg
      481 ggcagttgtg ctcttactac ctgcatgcct gcgggtacat tgttcagggt ataatttccg
      541 ttgatgtcgg tggtggtggt aagcaacgga aaaccatcta tagatacaaa ggcattgggt
      601 atgtttatag ctgtttgctg cacattgccg cttacggtgc cggtggtggc tgcgggtaca
      661 agggtaatgt cttgtgtggt tgtattgttt tcggcaacca ctatgcccgg tataatttgg
      721 gtggtatagc ccggtttgct tacctcaagg gtgtaggttc ccgggggcag caggccaaag
      781 ccgtatttgc ccaggctgtc ggtttgggtg ttaatgccta agccttgtat ggccacggta
      841 acagtatcta atttaaggtt ggtgaggctg tcggtaacaa agcctttgag cattagttct
      901 tcgtcaggct cttcgctgga gggcagcaga tagcggttat attttgcgta gttgtttttg
      961 taaatgagtt taccggtttt gcaaacgtag gtaactacat cccatactgc gttgtgcagc
     1021 tcgctgcggg tttgggtttt tacacctcgg ttgcctataa aggtatcttg ctgtatgttg
     1081 gctgctttta gtttatcggc aagcagttca atgccgtcaa tcattgcctg tgtatagttt
     1141 ttggctatca gttctgcgct gtattgcacg gccacggcat gaaatttata cataaacaaa
     1201 accattttgg gttgtgtttg ccgtgccttt tcataatcat taaagccaaa ctcgccctgc
     1261 accgatacgt tgttcggaaa agccttttca ataaagtgtt tggcattttg gtaataatcg
     1321 cggcactcct tcatggtagt gtgcaggtat tcggttttcc ccaccatttg gtccaccacc
     1381 gtttcgtcat caatatacgt ttcggcagcc tcaatttttg tttcccaatc cgtttcataa
     1441 gggttggcaa aatcgggatc aaaagccgta aaatcggcct gatcttctat aaagtaggct
     1501 cttttgttgt tggcatcggt aagcatgttg gcatcagaga tgtcgtattt tcttcctttt
     1561 tgttccggca tggtatgttt gtttttgtta gtattagtag tcagttcggg gttgctcgct
     1621 ataagggtta atgctgcccg ctatagcggt aaacagtaat cggtaatcgg ggaaacacaa
     1681 cacgctatat cggttatagc agctcgctac ttgggtaaat gctgcttgct accaggggaa
     1741 acagtgctcg ctattagggt agatgctgct cgctatcggg ggaaacattg ctcgccaaag
     1801 gaggagatac tgctcgcgat aggaggagat gctgctcgct acttggggag atgctgctcg
     1861 ctaccggggg agatgttgct cgccacaggg ggagacgctg ctcgccttcg gagatccttt
     1921 cccctgttgg tttttagcgt ttacccctct ggcgcggttt gtttcctttt gtggcgcgtt
     1981 gggtttagtc aaagaacgat tgggagcaaa actattgtct tgccataact cctgtaagcc
     2041 gattggtata aaccccttta ctttttaact gaaaccgtat aacaatgaat tgtaaactct
     2101 acccgatata aaaaccgcca tgcttttgtt tataatcaag gctcatcgga atttttactt
     2161 cctcataccc ctccagtttc aggttgggta ttttatgctt atcctgaaaa ataatgcagg
     2221 catggtttac cacctgattt ttgttcatgc gtccaaagcc aaagggcttt aatacagttt
     2281 caaaaaacag cagcgatttg atagatacaa tacgctcttt gcttgcaccg ctcaaataaa
     2341 tagtggtgat tttgtttata cactcaatgt aaacaatgtt ggcaacagga ataaaccggt
     2401 agccggtggg gatggggact cttaactttt tcatttgttt ttatttttca tccctgccct
     2461 gaattgagca gggtaattca aaaataaaaa gactgataaa aatttgtaag tttggggatg
     2521 cgcaaaggca gaaggaatgt agaataacga acgctcaata acgaatgccg aacactaaat
     2581 tcagaatgat gaaaaaacta ctcatccttc tcaataccat acttgtaatg ttcaattgca
     2641 cagcgcaaga aagcgatact gcatcggtat caacatccat aaaaatacaa aaacagcaag
     2701 gcggacgaac cctttatttg caaggcaata atgagctttc aaaaaaagag ttgctgtatg
     2761 tgttagaaga ctcgccctca gctatggaag agttgcgcta ttatgaactg gcaagaggag
     2821 taggctttgt tacaggagca gcaggcggct tttgcatagg ctggtactgg ggcggctata
     2881 ttatgggcaa acgatataac ccaacattac ttggtgtagg cgtagccgga gcactaacct
     2941 ctatcttatg cgagtttgca gcacgcaaaa atctgcacaa tgccattaac atctacaaca
     3001 acgaaacaca caaacccaca ggaagcaata ctcttcaact aaacttcgga ttttcagaac
     3061 aaggattagg attaaaccta aaattttgaa ctatgaaaaa actactactc ctcctcgtca
     3121 tgctgaactt gtttcagcat ctgctgttgg cgcagactat tattacctac aacacatatt
     3181 atctaatccc gccttcaagc ggttgtaatg gattgtgggc tgtcggccct tacaatccgg
     3241 cagagtgcaa tacaggaata tattcaataa atcctttggg atgtgcaact acttcaagca
     3301 catcaggtga taccttgttc ttaactcttt gctctattcc atgtgatttt atagcaatgg
     3361 atggcactgg tggtatatgc ttgaattgtt ttttggatta tgatttttcc acaactgtaa
     3421 ccgaatctgc cacaactgca ttcaccctct accccaaccc cgccagcacc actatcagta
     3481 ttctcccgat agctatcggg acaatggtca atagtccatg gtctcccgat agctatcggg
     3541 aagtcaatat ctacaacaca caagggcagc tggtaagtca tcccctctcg agaggggagt
     3601 gcgccagcct tagcgcaggc aaggggtgtg tgcttgacat ttcacaactc acccccggcc
     3661 tctactacct gcacctgcaa agcgcagaag gggtgggggt taagaagttt gaggtgatga
     3721 ggtaaaatcc atattcatta tatttgcaga aacatacacc atgaatttac aaaccgaaaa
     3781 agcccgcata aaagatgaac tggatggaat aaatgatgcc cgtcttatca aagccatccg
     3841 agaattactc aactatgcaa aacaaagttc agaagaacgt ttgcttaaac cttttacaaa
     3901 gcaacagctt gttaaacgtg cccttgcctc agaaaaagac attgccgcag gccgcaccac
     3961 taccctgaaa caactgcgca aagaggtaaa gaactggtaa actgatgcgg gtagaattaa
     4021 ccgactttgc aaaatcggaa ctcaggaata tttacaccta ttattcagaa gtttcaaccg
     4081 atgaaattgc atccaaaatt attgcacgta ttttagatga tattgaacta ttggaaaact
     4141 atccgctagc aggagcaaac gaacaacaac ttgccgaatt aggcagagaa cataaaaaca
     4201 tagtaagcgg aaattataaa atcattttcc gtattcataa aaccactgtt tacgttaccg
     4261 atatatttga ttgtcggcaa gacccaaaga aaatacctgc cagaaacagg taacccatgc
     4321 aagcccactt ccccatccgc tatggccgca actacaaagt agtaatggca attgtattgc
     4381 cctgcctgct catctggccc tttatagaag ccctgcagtt aataaagccg ctcgaggagt
     4441 ggcttttatg ggttagtatc tttggttttc tgggcatcat cagtgccttt agcgtgtggc
     4501 tggcaacccg cgtttatccc gaagccaccc tcagcatcca taacaatcag gtaagcctga
     4561 aattcaattc ctccaatttc ctgagccccg cagattttac cttcaccatt gccgacatca
     4621 cctcctttac ccgtggcgag gtaggcagcg atgaatacta catcatcatc acccaaaacc
     4681 caagacgcaa attccagctc tcggcctcca cctataaggt agaagacatg ctgcttttta
     4741 accaggcaat ggttgaaata agtgaaaagg taagaggcgt aaattcttag cgtctttgcg
     4801 ccttagcggt taaagcttct tcaacccagc aatagtcccc accgggtcgg cagaagaaaa
     4861 cacaaagtta cccgccacca atacatcagc ccctgcgttt ataagttgtg ctgcgttagt
     4921 gctacctaca ccgccatcaa tttcaatgcg tgcttttgag cctgtgctgt caatcagttt
     4981 gcgtagtttc tctaatttct gaaacgtgcc gggaataaat ttttgtccgc caaagccggg
     5041 gttcacactc atcaggcaaa ccagatctac atccgtaata atatcctcca gttggttaat
     5101 agccgtatgc ggattaaccg ccacacctgc ctgcataccc gcagctttta tcagttgcag
     5161 cgtacggtga aggtgagtgc tggcctcaat atgtacggta agaatatcgg caccggcatc
     5221 tttaannnnn nnnnnnnncg ggctgcacaa tcatcaggtg cacatccagc ggtttggtag
     5281 catacttctt cacctgttta ataaccggaa acccgaaaga gatattgggt acaaacaccc
     5341 cgtccattac atccacatga aaccagtcgg cctcgctgcg gttaatcatt tccacctctt
     5401 cacgaagttt agagaaatct gctgaaagaa ttgaaggagc tattaatgcc acggattaga
     5461 agttagaggt tagaagttgg atcttgtaga tttcgatgtc accctgagct tgtcgaaggg
     5521 cacaacctta ccctaaatat gtcttca
//
LOCUS       DAOOOI010000116         5527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2791_length_5527_cov_7.901316, whole genome shotgun sequence.
ACCESSION   DAOOOI010000116 DAOOOI010000000
VERSION     DAOOOI010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2791_length_5527_cov_7.901316"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1057
                     /locus_tag="PKX59_10130"
     CDS             35..1057
                     /locus_tag="PKX59_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOZ91264.1"
                     /translation="MGLFDFLTQEIAIDLGTANTLIIHNDKVVVDEPSIVAIDRMTGK
                     VLAVGKQAMMMHGKTHENIKTIRPLKDGVIADFDAAENMIRGMIKMINPGRRFFQPSL
                     KMVICIPSGITEVEKRAVRDSAEHAGAKEVYLIHEPMAAAIGIGIDVEEPMGNMIIDI
                     GGGTSEIAVIALGGIVCDQSIRVAGDGFTMDIWDYMRRQHNILIGERSAEMIKIEVGS
                     ALPELDNPPHDYAVHGRDLMTGIPKEITVTYSEIAHALDKSISKIEEAILKALEMTPP
                     ELSADIYRTGIYLTGGGALLRGLDKRIGMKTKLPVHVAEDPLRAVVRGTGIALKNIDR
                     YKFLIP"
     gene            1419..1598
                     /locus_tag="PKX59_10135"
     CDS             1419..1598
                     /locus_tag="PKX59_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91265.1"
                     /translation="MEEQENKKKASGLQVVMDDVMLLLFLGVTIYAIFYIIWGIMEIA
                     TVPNIPESVKQSILK"
     gene            1619..2548
                     /locus_tag="PKX59_10140"
     CDS             1619..2548
                     /locus_tag="PKX59_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOZ91266.1"
                     /translation="MTLIAPTEGWFKKKVSKDERMWIIISFVVCIFLFAWMVLWHVFG
                     RQNPSNTTYKTNPIEFAQLHDEYVKRNMVGMDNGIPIVKPEPGSDVILMAQMWRWSPV
                     LILKKDEWYNIHISSKDLVHGFSLQPSNMNFQVYPDYDYVLKFKPTVAGEAKVLCNEF
                     CGIGHHTMIGKILVIENDADLAKYGYNNLSAPPARTGKGTAEEDISGWSEEQLIKGGE
                     MVYNLKGCMACHKTDGTSEIGPAFNSIYGSKVKVKEDGNAKEIVIDDDYITTSIREPQ
                     KQIVVGFEKTPMAQMNLTDNEIKQVISYIKSIK"
     gene            2561..4228
                     /locus_tag="PKX59_10145"
     CDS             2561..4228
                     /locus_tag="PKX59_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOZ91267.1"
                     /translation="MFRICDITGFKVDLQSEKFIRLNAVMAVVSLLLAITAAILLVFT
                     RYQPVHLLDAEWYYRLTTFHGLNALIYWIVFFEIAGLYFGSTVVLNQKFCSPTLGWVT
                     FLLMVGGMVLSNVMVLMGKADVMLTSYVPLKAHPLYYLGIILFAVGALIGVGLFFANI
                     VNAKRDGNVGETLPLFTYGLMTAAILAVITLASGAIIYIPTFLWSLGLIDSVDPAVYK
                     LIWWGLGHSSQQINVAAMVSIWYLVTFLALGGTSINERVSRTAFFLYILFISLASAHH
                     LLTDPATSSAWKVWNTSYAMYLAVLASMIHAFAVPSSIESAQRRWGFNKGLFEWLSKA
                     PWSNPAFAACVLACIGFGFIGGTTGVIFGMEQTNIIVHNTIAIPGHFKGTVVIGTTLT
                     FMGITYYLIPLIFRRKIVAFKLAQLQPWMFFFGVAILSVSMIALGQLGVPRRHWDSTF
                     SGGPFTHEFNPSVDFFWTLMMLGGTLAFLAALIWVLQVVVSVFFGPKVNGPEDMQLPL
                     SPMAPAAKEHKGYEAPGTLILTLLFLATFAVLFFVNWKWLAATWWVK"
     gene            4259..>4916
                     /locus_tag="PKX59_10150"
     CDS             4259..>4916
                     /locus_tag="PKX59_10150"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOZ91268.1"
                     /translation="MINNKMQLTGLVILLSLFFSLSSFDIPDEKKFIGREVKNIPILD
                     SKGNNLQLFDYPTGKPIVLSPVYTRCPMACSLISNGLKTSIEKLGTLGKDFTVISFSF
                     DSTDTKEDLSGFEARWKMDGVNWKTVTSNAAGIGELLRSIDYHYEYDEKSKEYLHPNL
                     VVVITPSGRISRYIYGVEPKAIDLELAIMEAQVEKTSFNNFFKTLYIRCFTFNPATKS
                     Y"
     assembly_gap    4917..4982
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5022..>5527
                     /locus_tag="PKX59_10155"
     CDS             5022..>5527
                     /locus_tag="PKX59_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HOZ91269.1"
                     /translation="MINNKSSHWLFQRFAGIFSRIYSSKLNPLYHLGDIAILMFIVAC
                     ISGVYLFLFYNIDPAHAYESVEKISANPFNAVIRSIHRYSSDLLVIFIVLHFLQTLLT
                     GKFKRILSWVSGIISLMVVLIIGVTGFILVWDEKGKLVGYLTAKFFSAIPLFDPSIAG
                     SFISNNLDV"
BASE COUNT         1673 a         1009 c         1040 g         1739 t
ORIGIN      
        1 aactataaag gaaaacacta acaataaaag aaaaatggga ctatttgatt ttctgaccca
       61 ggagatagcc atagaccttg gcacggctaa cacgctaatc attcataacg ataaagttgt
      121 ggttgatgag ccaagtattg ttgctattga ccgtatgaca ggtaaggtac ttgctgttgg
      181 taagcaggct atgatgatgc atggaaaaac acatgaaaat attaaaacca tccgtccatt
      241 aaaagatggt gtaattgccg attttgatgc tgcagaaaat atgattcgcg gaatgattaa
      301 aatgatcaac cccgggagaa gattttttca gccatcgctt aaaatggtta tctgtattcc
      361 atcgggaatt acagaagtag agaaacgagc agtgcgtgac agtgcagaac atgccggagc
      421 aaaagaagtt tatcttattc acgaacccat ggcagcagcc attggtattg gcattgatgt
      481 agaagagcca atgggaaata tgattattga cattggtggt ggcaccagtg aaattgcagt
      541 tattgctctt ggaggaattg tttgcgacca atctattcgt gttgccggtg atggttttac
      601 gatggacatt tgggattata tgcgcagaca acacaatata cttattggtg agcgtagtgc
      661 tgaaatgatt aaaattgaag taggctctgc attgcctgag ttagacaacc ctccacacga
      721 ctacgcagtt catggtcgtg atctgatgac cggtattcca aaagaaatta cagttaccta
      781 ctcagaaatt gcacatgcac ttgataaaag tatcagcaaa attgaagaag ccattttaaa
      841 agcacttgaa atgacacctc cggaattatc agcagatatt taccgtacag gaatttacct
      901 tacaggtggt ggtgctttgc tgcgcggatt ggataaacgt attggaatga aaacaaaact
      961 tcccgtacac gttgccgaag acccacttcg tgcagtcgtt cgcggtacag gaattgcact
     1021 taaaaatatt gacaggtata aattcctgat tccataaaca tattgtaaac tcaaataaaa
     1081 agaggttagc tttataaaaa gctaacctct ttttatttgc ctggaacttt acttctttct
     1141 tcaacaattc tattcgtttg aattttataa agcaattgac tacatactgt ctttaaaaaa
     1201 tctacgtgtt cagtataatg aaactgaaaa gttattctca aaaaataatc ttactaaata
     1261 aaagaacaag aatggtttag ctaactactt tgatgtttaa actttcaaca atcattttct
     1321 tttatgacac aagtcatact taaaaatgct ttactgaaat aatcttgtca tcgaaaagta
     1381 aaatttcgat taataatcaa ttaaaaacca aaaattatat ggaagaacaa gagaataaaa
     1441 agaaggcttc cggacttcag gtagtcatgg atgatgtcat gttacttctt tttcttggag
     1501 taacaatcta tgcaatcttt tacatcatct ggggaataat ggagattgcc acagtgccta
     1561 atattcctga atcagttaaa caatctatac ttaagtaaaa ttctaaaaca atctatttat
     1621 gacattaata gcacctactg aagggtggtt taaaaagaaa gtttcaaaag acgaacgcat
     1681 gtggataatc atatcgtttg tggtttgtat ttttcttttt gcatggatgg tactgtggca
     1741 cgttttcggt cgtcagaatc catcaaacac cacatataaa actaacccta ttgaatttgc
     1801 ccaactgcat gatgagtatg taaaaagaaa catggtgggc atggacaatg ggatacctat
     1861 tgtaaaaccg gagcccggca gcgatgtcat tcttatggca caaatgtggc gatggtcacc
     1921 ggtacttatt ttaaagaaag atgagtggta taacattcac atttcttcaa aagatttagt
     1981 acacggtttt tcattacaac cttcaaacat gaactttcag gtatatcctg attatgatta
     2041 tgtcttgaaa tttaaaccaa ccgttgccgg tgaagccaaa gtattgtgta atgaattctg
     2101 cggcataggt caccacacca tgattggaaa aattcttgta attgagaatg atgctgatct
     2161 ggcaaaatac ggttacaaca acctgagtgc accaccggca cgcacaggca aaggaactgc
     2221 tgaagaagac atttccggat ggagcgaaga acaacttatt aaaggtggtg agatggtgta
     2281 taatctgaaa ggctgcatgg cttgtcacaa aacagatggc acttccgaaa ttggtcctgc
     2341 tttcaacagc atttatggaa gcaaggttaa agtaaaagaa gatggcaatg caaaagaaat
     2401 tgttattgat gatgattata ttactacatc tatcagagag ccacaaaaac aaattgttgt
     2461 aggatttgaa aaaacaccaa tggctcaaat gaaccttaca gacaatgaaa ttaaacaagt
     2521 aatctcctac attaaatcaa ttaaataaaa tctaaagctt atgttcagaa tatgcgatat
     2581 aaccggattt aaagtagatc tgcaatcgga aaaatttatc agattaaatg ctgttatggc
     2641 agtagtttca ttgctcttag caattacagc tgctatattg cttgtgttta cacgatacca
     2701 acctgtacat cttttagatg ctgaatggta ctacagatta acaacctttc atggtttgaa
     2761 tgctcttatt tattggattg tatttttcga aattgcaggt ttgtatttcg gatccacagt
     2821 agtattgaat cagaaattct gctcaccaac attaggatgg gtaacttttt tactcatggt
     2881 aggcggtatg gttctttcca acgtaatggt attgatggga aaagcagatg taatgttaac
     2941 ctcttatgtc ccactaaaag cacatccact ctattacctt ggtataattc tatttgccgt
     3001 tggtgcttta ataggtgttg gtttattttt tgccaatatt gttaatgcaa aaagagatgg
     3061 aaatgttggt gaaactttac ccctctttac ttatggttta atgacagctg caattcttgc
     3121 tgttattacc ttagcatcag gagctatcat ttacattccg acctttcttt ggtctttagg
     3181 attaattgac tccgtagatc ctgccgttta caaactcatt tggtggggac ttggtcactc
     3241 ttcgcagcag atcaatgtgg ctgccatggt cagcatctgg tacttagtta ccttccttgc
     3301 gcttggcgga acatcaatca atgaaagagt ttcacgaaca gcattcttcc tctacattct
     3361 ctttatcagt cttgcatccg cacatcactt gttaacagat cctgcaacat catcagcatg
     3421 gaaagtatgg aacacttctt atgcaatgta tcttgctgtg ttagcatcaa tgattcatgc
     3481 ttttgccgtt ccaagttcta ttgaatccgc acaacgcaga tgggggttca acaaagggct
     3541 gtttgaatgg ctttctaaag caccatggtc aaacccggct tttgcagcat gtgttctggc
     3601 ctgtattggt tttggattta ttggcggtac aaccggtgta attttcggta tggaacaaac
     3661 aaacattatt gttcacaaca ccattgcaat tcccggtcac tttaaaggca ctgtagttat
     3721 tggtactaca ctcactttta tgggtatcac ctactacctc attcctctta ttttcagaag
     3781 gaaaattgtt gcattcaaac tggcacaatt gcaaccgtgg atgtttttct ttggcgttgc
     3841 catcctatct gtatcaatga ttgccttagg tcagttaggt gttccacgca gacattggga
     3901 ttcaacattc agcggaggtc ctttcactca tgagtttaat ccatcagtag atttcttctg
     3961 gactttaatg atgcttggtg gtactttagc cttcttggca gctctcatct gggttttgca
     4021 ggttgttgtt tccgtattct tcggaccaaa agtaaacggg cctgaagaca tgcagctacc
     4081 actttcgcca atggctcctg ccgctaaaga acataaaggt tatgaagcac ccggaacatt
     4141 aattctcaca ctcttattcc ttgccacatt tgcagtattg ttctttgtta actggaaatg
     4201 gcttgcggca acatggtggg tgaaataatt tatttatctc ttttctaatt attggttcgt
     4261 gattaataat aaaatgcaac ttacaggctt agtgatatta ctaagcctgt ttttttcttt
     4321 atcctctttc gatattcccg atgagaaaaa atttattggt cgcgaggtaa agaatattcc
     4381 tattcttgat tcaaagggaa acaaccttca gctttttgat tatcccacag gcaaacccat
     4441 tgtgttgtca ccggtttaca cccgttgccc aatggcttgt tcgcttatca gcaacggatt
     4501 aaaaacctcc atcgaaaaat taggtacttt aggaaaggat tttacagtca tttcattttc
     4561 gtttgactca actgatacca aagaagattt gagtggattt gaagcacgct ggaaaatgga
     4621 tggtgttaac tggaaaacgg tcacctccaa cgcagcaggc attggcgaac tattaaggtc
     4681 aattgattat cattatgaat atgatgaaaa aagtaaggag tatttacatc caaacttagt
     4741 tgttgtcatt acacccagtg gtcgaatttc aagatacatc tatggcgttg agcccaaagc
     4801 tattgacctt gaacttgcca tcatggaagc acaagttgaa aaaacttctt tcaacaattt
     4861 ttttaaaaca ctttacatac gctgttttac ctttaaccct gcaacaaaat cttatcnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnaagtttat actccaataa cactgaagac agtgcagaat tatgattaat aataaatcaa
     5041 gccattggct ttttcaacga tttgccggca tcttcagccg aatttattct tctaagttaa
     5101 acccattata ccaccttggc gacattgcaa tactgatgtt tattgttgca tgcatcagtg
     5161 gagtttatct ttttttgttt tataatattg atcctgctca cgcctacgaa tcagtagaaa
     5221 aaatttctgc caatccgttt aatgctgtca tcagaagtat tcatcgttac agcagtgact
     5281 tattggttat ttttattgta ctccattttc ttcaaacctt attgacagga aaattcaaac
     5341 gtattctttc ttgggtaagt ggaattattt ctttgatggt agtgctcatt attggtgtaa
     5401 caggttttat tcttgtatgg gacgaaaaag ggaaattagt cggctacctt actgcaaaat
     5461 tcttttctgc aattccactc ttcgaccctt caattgcagg ttcatttatt tcaaacaatc
     5521 ttgatgt
//
LOCUS       DAOOOI010000117         5416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2854_length_5416_cov_10.869800, whole genome shotgun sequence.
ACCESSION   DAOOOI010000117 DAOOOI010000000
VERSION     DAOOOI010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2854_length_5416_cov_10.869800"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PKX59_10160"
     CDS             complement(<1..771)
                     /locus_tag="PKX59_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91270.1"
                     /translation="MRKISTLFAMLCITTAVSAQTQRLVLFEEFTGETCGPCALVNPT
                     LNEMLNDEETKIVSIKYQNNIPSPGPRFWPYAQSDIAARQAYYNNTYSPHGIMDGNQF
                     NDNAGALDPVIINPRYATPSPFEVRVAHTFSAANDTIYASVIIKAAQSYSDSNLVAQI
                     AVTERDIFGYTSPNGETHYEGVLRKMLPSAYGTPLQISWNAGDSVVLNYSWYITPAAL
                     SSPVYYQLSVVAFVQDNSSKEVLQAGFSRAQVPMDPKAK"
     gene            complement(835..2290)
                     /locus_tag="PKX59_10165"
                     /pseudo
     CDS             complement(835..2290)
                     /locus_tag="PKX59_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807008.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(2312..3784)
                     /locus_tag="PKX59_10170"
     CDS             complement(2312..3784)
                     /locus_tag="PKX59_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOZ91271.1"
                     /translation="MSKGIHVKKPLDSLLAEAEDSEKGLKRTLTANSLIALGIGAIIG
                     AGLFVRTAAAAANNAGPAVTLAFVIAGLGCAFAGLCYAEFASMIPIAGSAYTYSYTTM
                     GEFIAWIIGWDLVLEYALGAATVSIAWSEYLNKLLNYLGMHIPYEWCHSPFEHSLDGV
                     YGIMNVPALVILILLSLLLIKGTQESAFVNGIIVVTKVAIVILFIVFGWQFINPANHT
                     PYIPAASTFTDHMGVPHHYGGILGILGAAGVVFFAFIGFDAVSTAAQEAKNPGRDMPK
                     GILGSLIICTVLYILFAHVLTGVASVEDFRTQGREASVTYAIQTYMHGYDWLAKLVTV
                     AILAGFSSVILVMLLGQSRVFYSMSRDGLLPKVFSELHPVYRTPYKSNLILFVFVGLF
                     AAFVPGDIVGDMTSIGTLFAFMLVAAGVIIMRKTNPDAKRTFRTPLVPLVPILGIIVC
                     GAMIFGLGWPNWSRLLMWLAIGFVIYFGYSAKHSKLRNPK"
     gene            complement(3889..4698)
                     /locus_tag="PKX59_10175"
     CDS             complement(3889..4698)
                     /locus_tag="PKX59_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOZ91272.1"
                     /translation="MFRKIAHKAVSYNLLIALAASAASHLTILRINETNNVVLIAIIF
                     FATLAIYNFPRLPWQKPLNTRTWICLISILFSAILIINYNYISINLFLTAILACLIYM
                     MPFNYKGKRIKGIRNIFLLKNIFLAASWALITVLLPMAALGHESPIIKEVFLLRFIYV
                     FGISLLFDIRDTAKDKTRNHLTLPVVLGTTVTRYIVIASAALFILIVSVLYRHEHFTL
                     PMAVAFITSALLLVVATITAPPAANFKFYQVGVDSLLIAQWLLVVVAVKFY"
     gene            complement(4699..>5416)
                     /locus_tag="PKX59_10180"
     CDS             complement(4699..>5416)
                     /locus_tag="PKX59_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91273.1"
                     /translation="CYILHTFFIVLNQITDMTLRHFITSLIFLFSATTLSAQDTLLLL
                     NGKTKPVKLFNLNDPDWVRYVKFKESDKKKNEGSARVKRIDVYKVFSIKKADGTEQVL
                     YKPDSTSDDPDIPWVRDFIAGQQYGIMHKRERFNKHDNQWHRRLNFTEVGGIAAGGAG
                     SLLTFYGIPVPAIYAIAVGRHSPALPNDPAIAPEMKNSEGFISGYQKQRRNQRIRQGF
                     ISGMIGFAVGIVTFTLIERN"
BASE COUNT         1774 a         1052 c         1052 g         1538 t
ORIGIN      
        1 ttttgctttt gggtccatag gaacttgtgc acgactgaaa cctgcctgaa gtacttcttt
       61 gctagagttg tcctgaacaa atgcaactac tgatagctga taataaacag gagatgaaag
      121 tgcagcaggt gtgatgtacc acgaataatt tagaacaaca gaatcgcctg cattccatga
      181 gatttgcaat ggcgttccgt atgcagaagg caacatttta cgaagtactc cttcgtagtg
      241 tgtttcacca ttaggtgatg tgtatccaaa aatatctcgt tcggtaactg caatctgtgc
      301 aaccaagttt gagtcgctat aactttgtgc tgcttttatt attacagaag cataaatagt
      361 gtcgttggct gcactgaaag tatgagcaac cctaacttca aatggtgaag gtgttgcata
      421 acgtgggttg atgataacag gatctaaagc acctgcatta tcgttaaact gattgccatc
      481 cataattcca tgaggtgaat aagtgttgtt gtaatatgcc tgacgggctg caatatctga
      541 ttgtgcataa ggccagaatc gcggaccggg agaaggaatg ttgttttgat acttaataga
      601 aacaattttt gtttcttcat cattaagcat ttcgttgagt gtaggattta ccagtgcaca
      661 tggcccgcag gtttcaccgg taaattcctc gaaaagaaca aggcgttgtg tttgtgctga
      721 aacagcagtt gtaatacaca acatagcaaa tagtgtagag atttttctca tttgaatgac
      781 atttatattt tgaactacga atgtacgtag cttttaaata cctgagaaat ctatttaact
      841 gctgcgtctt ttaaacagat aatagatggg taatcctagc agaacaataa gtaagccttt
      901 gccggcaacg gctgttttgg tataaagtaa tatagagcaa attgatagtg ctaaaataat
      961 atagagtgcc ggtaaaaatg gataaccaaa tactttgtaa ggcctttctg catcaggctc
     1021 acgtttacgc aaaataaaaa taccggaaat agttattaca taaaacagga gtgaggcaaa
     1081 aacagtgtaa tcaagtaaat caccatacgt gccgcttaga cataataaac ctgcccatat
     1141 tgcctgtacc catagcgcaa ttgctggaac tccttttgaa ttgagttttt tttgcctgct
     1201 taaaaaagag attttccaaa gccattgcat aataaactct ggcaccacta aggataatac
     1261 cattgttgca tccgaaagtt gaaatcatga tgagtgctgc catgatataa acacttgagt
     1321 caccaaaaat catagaggct gcggctgttc ctacccggtc ggtgtcgcct cctgcaaact
     1381 gaataccttg ccctgcaact gttgcagcat caatactgcc ttttaaaggt aataatgcca
     1441 gatatgcaat gttggctaag cagtaaagta aagttaccat tgctgtgccc aacatcagac
     1501 ttcgtggaat gttgcggctc ggatctttta tttcaccggc aataaatgta acgttgttcc
     1561 aagcatcgct gctgaataat gagcctataa tggcagtgcc aagcatgagt attattgcaa
     1621 agccactgat gtgttgttct atccagcccg aatcggtttt aatcattgaa ttaacgctcc
     1681 aggcattgcc gaagttagtt gaaagcgtgt tggtttttaa tccaacccat agtccggcta
     1741 caatgagaat aaatagggca agcaactttg ctgaagtaaa aatgttttga atggtttttc
     1801 caccttcaat accacggctg ttgaaccatg ttagtacagc aatgagaaat atagcaagca
     1861 tttgtgcagc agatatactt attccgcctg cctgaaataa aatattgcct gtgttaaaat
     1921 aaggaaaaaa cacagcagta aattttgcaa atgctacagc cactgcagca ataactcccg
     1981 tttgaatgac cataaatact gtccatccgt ataaaaatgc caagcagttt ccccatgcac
     2041 gtctgatata aacaaactga cctcctgctt gcggcatcat acctgccagt tcgccataac
     2101 aaagtgctgc tataagtgtg ataacaccac ttagcaacca taaaaaaagc aaccatcctg
     2161 cagaacctgt atgccgtgac atgtcggcac tgacaataaa aatacctgag ccaatcattg
     2221 aaccggcaac caacatggta gcatcccaca gtcctaatga acggtgcagg tgtggtttgt
     2281 cgttgtgcat ataaattttt agaaagtaat tttatttcgg attacgtaat ttactgtgct
     2341 ttgcactgta gccaaaataa ataacgaaac ctatagccag ccacattagt aagcgtgacc
     2401 aattaggcca acccaagccg aatatcatgg ctccacaaac aataattcct aagatgggaa
     2461 ctaatggaac taaaggtgtt ctgaaagtac gttttgcatc gggattggtt ttacgcataa
     2521 tgattacacc ggctgcaacc agcataaatg caaacagtgt tccaatgctt gtcatatcac
     2581 caacaatatc acccggtaca aatgcagcaa aaagtccgac aaaaacaaag agaattaaat
     2641 tcgatttata aggtgttcgg taaacaggat gtagttctga aaaaacttta ggcaacaatc
     2701 catcacgact catagaataa aatacacgtg attgtcctaa aagcataact aaaattacag
     2761 aggagaatcc ggcaagaata gcaacggtta ccaattttgc taaccagtcg tagccatgca
     2821 tataggtttg aatggcataa gtcactgagg cttcacggcc ttgtgttctg aaatcttcta
     2881 ctgaggcaac tcctgttagc acatgagcaa acagaatgta aaggacagta caaattatta
     2941 atgaacctaa aattcctttt ggcatatcac gaccgggatt ttttgcttct tgtgcagcag
     3001 tgctcacggc atcaaaacca atgaatgcaa agaataccac accggcagca ccaagaattc
     3061 ccaatattcc accatagtga tgtggcacac ccatgtggtc ggtgaaagtg cttgcagcag
     3121 gtatataagg cgtgtgatta gcaggattaa taaattgcca gccaaaaaca ataaagagta
     3181 ttacaatagc cactttagta actacaatga taccatttac gaaggcactt tcttgagtac
     3241 cttttattaa gagtaaggaa agtaatatga gaattaccaa tgcaggaaca ttcataatac
     3301 cataaacacc atctaaacta tgttcaaaag gtgaatggca ccattcataa ggtatgtgca
     3361 tgcccagata attcaacaat ttgttcaggt attcgctcca ggcaatactc actgttgctg
     3421 caccaagtgc atattcaagc acaagatccc atccgattat ccaggcaata aattcaccca
     3481 ttgtagtgta actataagta taggcactac cggcaatagg tatcatactt gcaaattctg
     3541 catagcacaa tcctgcaaag gcgcaaccca atcctgcaat aacaaaagct aatgtaacag
     3601 ccggcccagc attgtttgca gcagcagcag cagtgcgcac aaatagtcct gcaccaataa
     3661 tagcaccaat accgagtgca ataagactgt ttgcagtaag cgttcttttt aaaccttttt
     3721 cgctgtcttc tgcttcagct aataaactat cgagaggttt ttttacatgt attcctttgg
     3781 acataccttt gatcttgatt tttagtggag ttcaaaccta catgattaag attaaattac
     3841 caaaaaaata aaagaggatt tgtataacga ggagggaata gaaagaaatt aataaaattt
     3901 tacggcaact accaccaata accattgtgc aataagcaaa ctgtcaactc caacctgata
     3961 aaatttaaag tttgctgcag gtggtgcagt aattgtcgca actaccaaca acagtgcact
     4021 tgtgataaat gcaactgcca taggtaatgt aaaatgttca tgacgataaa gtacagaaac
     4081 aattaaaata aataatgctg cagatgcaat aacaatatat cgggttactg ttgttcctaa
     4141 aaccacgggt agtgtcaggt ggttgcgtgt tttgtctttt gcagtatcgc gaatatcaaa
     4201 caataaagaa ataccgaata cataaatgaa acggagtaaa aaaacttctt ttattattgg
     4261 tgattcatgc cctaatgcag ccataggtaa gagtacagtt attaatgccc aacttgctgc
     4321 aagaaaaata tttttaagaa gaaaaatgtt gcgaattcct ttgatgcgtt tgcccttgta
     4381 attaaaaggc atcatataaa tgagacaagc cagaatagct gtcaaaaaaa gatttatgga
     4441 aatataatta taattgatta ttaaaattgc tgagaataga atcgaaataa gacaaatcca
     4501 tgtccgtgtg ttaagtggtt tttgccatgg caaacgagga aaattataga tagccagagt
     4561 agcaaagaat ataattgcta taagaactac attgtttgtt tcatttattc tgagaatagt
     4621 taaatgactt gctgcacttg ctgcaagggc aatgagtagg ttataggaaa cagctttatg
     4681 agcaatcttc ctgaacaatc agttccgttc tattagagtg aaagtaacaa tgccaacagc
     4741 aaaacctatc atgccggaaa taaatccttg tctgattcgc tgattacgtc tttgtttttg
     4801 atagcctgaa ataaatcctt cgctgttttt catttcagga gcaatagccg gatcgttggg
     4861 taatgcaggg ctatgccgtc ccacagcaat ggcataaatt gcaggcacag gaataccata
     4921 aaaagttaaa agacttcctg caccgcctgc agctattcca ccaacttcag taaagtttag
     4981 tctgcggtgc cattggttat cgtgtttgtt aaaacgctct cttttatgca ttattccata
     5041 ttgttgtcct gcaataaaat cgcgcaccca tggaatatca ggatcatctg atgtgctgtc
     5101 cggtttataa agaacttgct ctgtgccatc agcttttttg atggagaaca ctttgtaaac
     5161 atcaatgcgt tttacacgcg cacttccttc atttttcttt ttatcactct ccttgaattt
     5221 tacatatctc acccagtcgg ggtcgttgag gttaaaaagt tttacaggtt tggttttgcc
     5281 attaaggagc aacagtgtgt cttgtgcgct taatgttgtg gcgctgaaaa gaaaaataag
     5341 ggatgttata aagtgacgta aggtcatatc agttatttga ttcaaaacta taaaaaatgt
     5401 atgtaatata tagcat
//
LOCUS       DAOOOI010000118         5370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2880_length_5370_cov_7.498777, whole genome shotgun sequence.
ACCESSION   DAOOOI010000118 DAOOOI010000000
VERSION     DAOOOI010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2880_length_5370_cov_7.498777"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="PKX59_10185"
     CDS             <1..1026
                     /locus_tag="PKX59_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOZ91274.1"
                     /translation="QSVFIFLVCFLIFSCKKDKPETIIQPLITSNNNGVVYITNEGNF
                     QFGNAAISLYDKESKNIIEDYYKQVNQVPLGDVCQSMTYFNNKFYIVVNNSSKVMVVN
                     AATFKIEATISGFNSPRYFIPVSNSKAYVSDLYDNRLTIVNLSTNTISGYIPAVAGTE
                     QMKMVYGKVFVSSLFTDKVYVINAMTDMLTDSITLAYSPNSICEDKNGILWVLCSGDS
                     LKQKPSALIQMNPLNNQVIKSFVFTGNHAPWRMCMNQQNDTLYFLDRHVWQMSISDTT
                     LPSTPFINGNGKLFYTLSVDASNGEIYVSDAIDYLQRGSIYRYSASGNLIDQFKAGII
                     PGGFCFR"
     gene            complement(1057..1130)
                     /locus_tag="PKX59_10190"
     tRNA            complement(1057..1130)
                     /locus_tag="PKX59_10190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1094..1096),aa:Arg,seq:tct)
     gene            complement(1188..1263)
                     /locus_tag="PKX59_10195"
     tRNA            complement(1188..1263)
                     /locus_tag="PKX59_10195"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1225..1227),aa:Pro,seq:tgg)
     gene            complement(1355..1443)
                     /locus_tag="PKX59_10200"
     tRNA            complement(1355..1443)
                     /locus_tag="PKX59_10200"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1407..1409),aa:Ser,seq:gct)
     gene            1627..3387
                     /locus_tag="PKX59_10205"
     CDS             1627..3387
                     /locus_tag="PKX59_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ91275.1"
                     /translation="MAETNSTSGFIAIPDAKAWDPAWSTENKVVYQVLQEPDNLHPTN
                     GKTLMRSEVMLYTQGVLIGTDFKTNSLKPNLLSKLPVYDANKSSYNFHLRNDVQWDNG
                     EHLTANDILFTLKVYKSALTQNDFFKPYLENMLDFIPDVNDSLAFSIKIAKPYLQDVA
                     IWGDIIIMQQSFHDPKNILAKYSLTTLATEDFRANAPADVKKWFEEFNSEATGFDLKK
                     LNGLGPYKVTAWEHGQFLTLEKKNNHWTDHSKDSSEHSSVAKIIFKISSDPNVNVLEF
                     KKQQFDASTTLATKSLLKLREDTLFNHNYNAQFTSTFNYSFAAFNMKPDGKKHKKLFD
                     NLQVRKAMAMLFPYEQINRALYKGEYKRQAGPVCSHKYNFNESLTPILQNYNEATKLL
                     ADAGWKDSDNDGVLDKMIDGIKEDFKFDMNIYAGIPDWNDLSLMLVAELKKAGIVMNI
                     VPLEVPVFLEKARTHDFDMLMSVWTTNVSPDDFTQLWHSSSWRENGDNYSGFGNSASD
                     ALIDSIKFTIDDSLRTALDKRFQKIVVDDMAYIFLFNSQRRIAVHKRFGNVNLYFEKP
                     GLLINTFRLLSPVNKTSATG"
     gene            3469..4830
                     /locus_tag="PKX59_10210"
     CDS             3469..4830
                     /locus_tag="PKX59_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ91276.1"
                     /translation="MLSVYLPGDALDYILIREEKTSITSTNYEHQKTLLAQRLGYDKP
                     LFYFSVISLAEYGWQQYLPSEKQQKSYNHLLRNFGCGSEVFIFYKKINAARTAITTSF
                     ASATDTAIRQTASNAANSLFQIETQVKLSKNDSILDSFNNYATILKLNSLANSILHSY
                     NAMQTKKSSWKKWVPAVQLNAQNRFHDWFFGTDKNSGIIHLNLGQSLYTGRSIGSLLK
                     EKIFWSLLLTVLAIIIAFISGLLISIWLAYTKNKIASRIINMITVLFYSLPVYFAGTA
                     LLYFFSNPDFLQIFPSSGVMPIGGYSSSASFSEKFIGTLPYLILPLICYTYSLWAFVQ
                     RTSASLISAEFKKPYALMAQAKGLTKKEVVIKHIFPNIISPFVALLANMFPAVIGGSV
                     IIESLFSIPGMGLELIHATTTRNYPFISAVILITGLITILLYLLNEWIQHYVDPRTKT
                     VQP"
     gene            4954..>5370
                     /locus_tag="PKX59_10215"
     CDS             4954..>5370
                     /locus_tag="PKX59_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91277.1"
                     /translation="MIYHSELRAKAIIKFCALPYSPGQPRQNDIALLSPFQKSYYTNN
                     TGQQIELPLRFHHWLGTDAIGADVAAGIVFGLRHSLIIALFSMIFSIVISLIAGICSG
                     YFADSGIYLNRIKFVSLIIFLFLVWFYGFYTFSMEWH"
BASE COUNT         1706 a          997 c          942 g         1725 t
ORIGIN      
        1 caatctgtat ttatatttct tgtttgcttt cttatttttt cctgtaaaaa agacaagcct
       61 gagacaataa tacaaccttt aattacttca aacaataatg gagtggtgta tattaccaat
      121 gaaggaaatt ttcagtttgg aaatgcagca atcagcctgt atgataaaga atcaaaaaat
      181 attattgaag actattacaa gcaggtaaat caagttcctt taggtgatgt ttgccagagt
      241 atgacttact ttaataataa gttttacatt gtagtcaaca attcttctaa agtgatggta
      301 gttaatgctg caacttttaa aattgaagca actatttcag gttttaattc accacgttat
      361 tttattcctg tcagcaacag caaagcttat gtatcggatt tatacgacaa cagactgacc
      421 attgtaaatc tttctaccaa caccatatca ggttatattc ctgcagttgc aggcacagaa
      481 caaatgaaaa tggtttatgg aaaagttttt gtttcatcac tcttcactga taaggtttat
      541 gtcatcaatg cgatgacaga catgctgacg gatagcataa ctttagctta ttcgcccaat
      601 tctatctgtg aagataaaaa cggcatatta tgggtgctgt gcagtggtga ttcgttaaaa
      661 caaaaaccat ctgcattgat acagatgaat ccgttaaata atcaggttat taaaagtttt
      721 gtttttaccg gcaaccatgc cccctggcgt atgtgtatga atcagcaaaa tgacactctt
      781 tattttcttg atcgccatgt atggcaaatg agcatcagcg acactacatt accttcaaca
      841 ccattcatca atggaaacgg aaaattattt tacactttga gtgttgatgc ttcgaatggt
      901 gaaatttatg tatcggatgc aatagactat ttgcaacgtg gaagtattta tcgttattct
      961 gcttcaggaa atttgataga tcagtttaaa gcaggaatca tccccggagg attttgtttt
     1021 cggtaaaatt tgattcggga tgactaagaa aagttgtgat cccgattgga ttcgaaccaa
     1081 tgacctactg cttagaaggc agttgctcta tccagctgag ctacgggacc aaacagccgt
     1141 aaacataaaa gtctaaagcg gcaaccgctt tagactctct ttttttgtcg gggtggcagg
     1201 attcgaacct gcgacctcct gctcccaaag caggcgcgat gaaccgggct acgctacacc
     1261 ccgaaaattt ttaaattcgg ttacgactca aagaacaact ttcatgcgaa gaaacatttt
     1321 cttcacctta cacctgattt tcgcaaaact tttgcggtga gggtgggatt cgaacccacg
     1381 gtacaggttg ccccgtacga cagtttagca aactgttcct ttcggcctct caggcacctc
     1441 accttttcag atgggcggca aaggtaaaaa aaagtatatg caaacaaaac tatatgcttt
     1501 aattttattt ttttatgcct cttatatgct aatattgcct ttatgagagt tgtcggtatc
     1561 aacattttat tcgcattttt gattttaatg gtctcttgca cagaccacaa gccgcagaat
     1621 tacactgtgg ctgagacaaa cagcacttca ggattcattg ctattccaga tgcaaaagca
     1681 tgggatccgg cttggagtac agaaaataaa gtcgtgtatc aggtacttca ggagcccgac
     1741 aatctgcacc caacaaatgg taaaaccctg atgcgtagtg aagttatgct ttatacgcaa
     1801 ggtgtactga ttggaacaga ttttaaaaca aattctttaa aacccaatct gcttagtaaa
     1861 ctacctgttt atgatgccaa caaaagttca tataacttcc atttaagaaa tgatgttcaa
     1921 tgggacaatg gtgaacacct tactgccaat gatattcttt ttactttgaa agtttacaaa
     1981 agtgcactta cacagaatga ttttttcaag ccttacctag aaaatatgct tgactttatt
     2041 cctgatgtaa atgattcgct tgccttcagc ataaaaattg caaaacccta tctgcaggac
     2101 gttgccatct ggggtgacat tattattatg caacaatcat ttcacgatcc aaaaaatatt
     2161 cttgcaaaat actccctgac aactttagct accgaagatt ttcgagccaa tgcaccggca
     2221 gatgttaaaa aatggtttga agaatttaat tccgaagcaa caggttttga cctgaaaaaa
     2281 ctaaatgggt tagggccata taaagttact gcatgggaac atggtcagtt tctaacattg
     2341 gagaagaaaa acaatcattg gacagatcat tccaaggatt catcagaaca ttcttctgtc
     2401 gcaaaaataa ttttcaaaat cagcagcgac ccaaatgtga atgttcttga atttaaaaag
     2461 cagcaatttg atgccagcac aacacttgca acaaaatcat tactcaaatt gcgtgaggat
     2521 actttattca accataatta caatgctcag tttacaagta cattcaacta ttcatttgcc
     2581 gcttttaata tgaagccaga tggaaagaag cataaaaagt tatttgataa tttacaagta
     2641 agaaaagcta tggcaatgct ttttccctat gaacaaataa acagggcatt gtataaaggc
     2701 gaatacaaaa gacaggcagg accggtgtgc agtcataaat acaatttcaa tgaatcgctt
     2761 acacccattt tacaaaacta caatgaagcc actaaactcc ttgccgatgc cggttggaaa
     2821 gatagtgaca acgatggtgt acttgacaaa atgattgatg gcataaaaga agattttaaa
     2881 tttgacatga acatctatgc cggcattccc gattggaacg atctttcttt aatgcttgtt
     2941 gctgaattaa aaaaagcagg tatagtgatg aatattgttc ctcttgaagt acctgtgttt
     3001 ttagagaaag cgcgaacaca tgatttcgat atgcttatga gtgtgtggac aacaaatgtt
     3061 tctcctgatg attttacgca gctatggcat tcgtcaagct ggcgcgaaaa tggtgacaac
     3121 tattcaggat ttggcaacag tgcttcagat gcattgattg attcaataaa atttaccatt
     3181 gatgattctt tgcggactgc tttggacaag cgttttcaga aaatagtggt tgatgatatg
     3241 gcgtatattt ttctttttaa ttcacaacgc agaattgcag tgcacaaacg attcggaaat
     3301 gtaaatcttt attttgaaaa acccggatta ctcatcaata cttttcgatt actttcaccg
     3361 gttaacaaaa cttctgcaac aggataaata aaattcagac taaggaagat gcggcatatc
     3421 attttcaaat acttttttat tttttctgta ctgttggttt catcgtttat gctatctgtt
     3481 tatttgcccg gtgatgcatt ggattacatt ctcatacgag aagaaaaaac aagtattact
     3541 tcaactaatt atgagcacca gaaaacatta cttgcacaac gtctaggcta tgacaagcct
     3601 ttattttatt tttctgtaat atccttagct gaatatggat ggcaacaata tcttccttca
     3661 gagaaacaac aaaaaagcta caatcatttg ttgcgaaatt ttggttgtgg tagtgaagta
     3721 tttatttttt acaagaaaat taatgctgca agaacagcaa ttacaacatc attcgcttca
     3781 gcaactgata ctgcaatcag gcaaactgct tctaatgcag caaatagttt atttcagata
     3841 gaaactcaag taaaactgag caagaatgac agtattcttg attctttcaa caattatgca
     3901 actatactta aacttaattc actcgctaat tctattttgc attcctataa tgcaatgcaa
     3961 actaaaaaat catcatggaa aaaatgggtt cctgcagttc aacttaatgc acaaaatcgt
     4021 tttcatgact ggttttttgg aacagacaag aatagtggca tcatacattt aaacttaggt
     4081 caatcactct ataccggaag aagcattggc tcattactga aagaaaaaat attctggtca
     4141 ttgcttctga ctgttctggc aattatcata gcatttattt ccggcctttt aatttcaata
     4201 tggttggcat atacaaaaaa caaaattgcc agccgcatta tcaatatgat taccgtactt
     4261 ttttattctc taccggtcta ttttgcagga acagccttac tatatttttt ctctaatcct
     4321 gatttcttac aaatatttcc atcttcagga gttatgccta ttggaggata ttcttcaagc
     4381 gcaagttttt cagaaaaatt tatcggcacc cttccctatc ttatccttcc gctaatttgc
     4441 tacacctatt ccctgtgggc atttgttcaa cgtacatctg ccagcctaat ttcagctgaa
     4501 tttaaaaaac cttatgcctt aatggcgcaa gcaaaagggc taacaaaaaa agaagttgtt
     4561 atcaaacaca tttttccaaa tatcatatcg ccatttgtag ccttgcttgc aaatatgttt
     4621 ccagcagtta ttggcggttc ggtaattatt gaatcattgt tttctattcc gggtatggga
     4681 cttgaactga ttcatgctac tacgacaaga aactatcctt tcatttctgc tgttatttta
     4741 attacaggac taattacaat tttactttat ctgcttaatg aatggattca gcattatgtt
     4801 gacccacgaa ctaaaactgt tcagccatga ggaggaagtt gaaatttcgt ttagcaaaac
     4861 tttggctgct gacagttgta ctccttgcat tgtttgctcc atttattgct aatgaaaaac
     4921 cattttttgg aaatgacaat ctaacttctg actatgattt atcattcgga attgagagca
     4981 aaggcaataa tcaaattctg cgccctaccc tactcacccg gtcagccaag acaaaatgat
     5041 atagcgttat taagtccgtt tcaaaaaagt tattatacaa acaatacagg acaacaaata
     5101 gaattacctc tgcggtttca tcattggtta ggtacagatg caattggtgc tgacgttgct
     5161 gcaggaattg tttttggttt acgacacagt ttaattattg ccttgttttc aatgattttt
     5221 agtattgtaa tttcattgat tgcaggtatc tgttcaggat attttgctga ctctggaatt
     5281 tatttaaata gaataaaatt tgtttcacta ataatatttc tgtttctggt ctggttttat
     5341 ggcttttaca cattctcaat ggaatggcac
//
LOCUS       DAOOOI010000119         5341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2901_length_5341_cov_5.174045, whole genome shotgun sequence.
ACCESSION   DAOOOI010000119 DAOOOI010000000
VERSION     DAOOOI010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2901_length_5341_cov_5.174045"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..428
                     /locus_tag="PKX59_10220"
     CDS             150..428
                     /locus_tag="PKX59_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91278.1"
                     /translation="MKKLLLVFALGLGLTFAQAQTVATTNNKATTTHAAKATSVKSEK
                     STAADHTKKDGTPDKRFKENKNVKAQGPKKADGTPDMRYKSNKPAAKK"
     gene            complement(683..1014)
                     /gene="rplT"
                     /locus_tag="PKX59_10225"
                     /pseudo
     CDS             complement(683..1014)
                     /gene="rplT"
                     /locus_tag="PKX59_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
     gene            complement(1099..1296)
                     /gene="rpmI"
                     /locus_tag="PKX59_10230"
     CDS             complement(1099..1296)
                     /gene="rpmI"
                     /locus_tag="PKX59_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOZ91279.1"
                     /translation="MPKMKRNSSAKKRFSVTGTGKIKRKHAFKSHILTKKSTKRKRAL
                     TNAAIVHKSDEFNVKRMLSIA"
     gene            complement(1331..1828)
                     /gene="infC"
                     /locus_tag="PKX59_10235"
     CDS             complement(1331..1828)
                     /gene="infC"
                     /locus_tag="PKX59_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOZ91280.1"
                     /translation="MNGRIHSPQVRLVGDNVEPRIYDTQTALDMAQDMGLDLVEISNT
                     SNPPVCKIVDYQKFLYERKKKDKEQKAKAAKSVVKEIRFGPNTDEHDFNFKLKHAIKF
                     LEEGAKVKAYVHFKGRSIAYKEKGEIILLKFAQELEQYAKVEMLPKLEGNRMFLHLAP
                     LAKKK"
     gene            complement(1931..3532)
                     /gene="thrS"
                     /locus_tag="PKX59_10240"
                     /pseudo
     CDS             complement(1931..3532)
                     /gene="thrS"
                     /locus_tag="PKX59_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583779.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
     assembly_gap    3533..3542
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3543..3837)
                     /locus_tag="PKX59_10245"
                     /pseudo
     CDS             complement(3543..3837)
                     /locus_tag="PKX59_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218453.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
     gene            3928..>4132
                     /locus_tag="PKX59_10250"
     CDS             3928..>4132
                     /locus_tag="PKX59_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HOZ91281.1"
                     /translation="MHQTILIIDFGSQYTQLIARRLRELNVYCEIHPYNHLPEMSDSI
                     KGVIFSGSPFSVRDSQAPLIDTKS"
     assembly_gap    4133..4157
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4158..>4368
                     /locus_tag="PKX59_10255"
     CDS             <4158..>4368
                     /locus_tag="PKX59_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HOZ91282.1"
                     /translation="PVLGLCYGAQLLAHQHQGEVVASKIREYGRAILKINFDDEIFND
                     VSDNSQVWMSHADTIRSAGNDFEVIA"
     assembly_gap    4369..4393
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4394..>5341
                     /gene="guaA"
                     /locus_tag="PKX59_10260"
     CDS             <4394..>5341
                     /gene="guaA"
                     /locus_tag="PKX59_10260"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOZ91283.1"
                     /translation="GFQYHPEVYHSTDGKKMLANFVYNICGCRGDWTPSSFVDETVAQ
                     LQDKLKNDKVILGLSGGVDSSVAALLLHKAIGKNLYCIFIDNGLLRKNEAQHVMEVFK
                     PLGLNIKCVDAATVFYDALKNISDPEQKRKTIGRVFIEVFDKEAHAISDVKWLAQGTI
                     YPDVIESVSVNGPSATIKSHHNVGGLPEKMNLKIVEPLRSLFKDEVRKVGKEMGLKEE
                     LLNRHPFPGPGLGIRILGEISAEKVKMLQEADHVFISLLQKHNLYQKVWQAGAIFLPV
                     NSVGVMGDERTYEIAICIRAVTSTDGMTADWAHLPYEFL"
BASE COUNT         1505 a         1019 c          998 g         1759 t
ORIGIN      
        1 gttatagtct aacaatggtg ttattttttt atgcaagagt gatagtcgga aattaatatc
       61 acttcagaaa aaatacatgc gttttaaaaa taaattcatg tggtggtcat ctgtgccggt
      121 tcatatttgt tgcataaatt tcaaaaaaaa tgaaaaagtt attattagta tttgcattgg
      181 gattaggttt aacctttgcc caggcacaaa ctgttgctac taccaataac aaagcaacaa
      241 caactcatgc tgcaaaagct acttcagtaa aatcagaaaa gagtactgct gcagatcaca
      301 caaaaaagga tggtaccccc gacaagcgct ttaaagagaa caaaaatgta aaagcacaag
      361 ggccaaaaaa agcagatggc acacctgaca tgcgttacaa atcaaataag cctgctgcaa
      421 aaaaataaag taagcgtttg aattatttta aaggctgttg cacgttatgc aacagctttt
      481 ttgtttttat tattttgata caacttataa caatgttgat gatagacgaa ataagaacat
      541 aatttaaact gaagaatttt tcaatttgaa tgctaacaaa atcaccattc ttgttaatac
      601 tttataagtt gtgtaaatta aattttaccc acaaaaaaaa gctgcccttt cgaggcagct
      661 tttacaaaaa tgtattgtca atttatttta cagactcaac aattgcttta aaagcatcag
      721 gattattaag tgcaaggtcg gcaagaactt tgcggttaag ttggttacct gctatatgca
      781 gtttacccat aaattcgctg taagacatat cgtaatttct tacctctgca tccataagcc
      841 gcggaagttg cgtttcttgg ttttacgatc gcggtatgca tattgcaaac ctttttctac
      901 tgtgtgtttg gcaacggtta atacgttacc tcttgcgccc caatttcctt tggcgcggtt
      961 gagcagtttt ttgcgtcttg ctcttgatgc tactgaattt accgaacgtg gcatgtgttt
     1021 tgaatttttg gcgttagcgc tccgctttgc ggaatcttta tgctaattac ccggttaaac
     1081 ttaattgaat attatttttt atgctattga aagcattctt tttacgttaa actcgtcaga
     1141 cttgtgtacg atggcagcat tggttaatgc acgcttacgc ttcgttgact tcttagtcag
     1201 gatatggctt ttgaaagcat gctttctttt tattttacct gtgcctgtaa cagagaatct
     1261 ttttttggca ctggagtttc ttttcatttt cggcataacg cttctaattt ttatttgttt
     1321 attattttat ttacttcttc tttgcaagtg gtgccaggtg cagaaacatg cggttgcctt
     1381 ctaatttcgg aagcatttca acctttgcat attgctccaa ttcctgtgca aacttcagga
     1441 ggataatttc tcccttttct ttataagcta tgcttcgtcc tttaaagtga acataagctt
     1501 taacttttgc tccttcttcg aggaatttga tggcatgttt taatttgaaa ttaaaatcat
     1561 gttcatcggt attcggcccg aagcgtattt cctttacaac agatttagct gcctttgctt
     1621 tttgctcttt gtctttcttt tttctctcgt aaaggaattt ttgataatca actattttac
     1681 aaaccggagg gttacttgta tttgatattt ccaccaaatc gagacccata tcctgcgcca
     1741 tatccagtgc agtttgtgta tcataaattc tgggttcaac gttatcgcca accaatctaa
     1801 cctgtggtga atgaatgcga ccattaatgc ggtgctcttc aactttagga cgtggtaatc
     1861 ttctgtcagg aaaacgacca cctggtttgg gcgtatttgg tttgttaaat gttgctatat
     1921 agcctccttt ttacttgttt attaataatt gatcttcaat ttctttcttt actaattgac
     1981 aaaatgcctc aggtgtcatg cttcctttat ctccttcgcc atgcttacgc acagatacca
     2041 gtttttcatt catctctttc tccccgacaa tcagcataaa cggtactttt ttaacttccg
     2101 catctctgat ttttctgcct attttctcac ccctttcatc aacgagggcg cgaatatcgt
     2161 aattatttag taactgtgaa acagattttg caaaatcatt gaacttttcg cttattggca
     2221 gtattgctac ctgcataggt gcaagccaca aagggaaatt acctgcataa tgctcaatca
     2281 aaaatccgat aaagcgctca tgtgttccca gcggtgcacg gtgtatgcaa agtggtgttt
     2341 tcattgtatt gtcggcatca cgataggtaa gcccaaaacg tgcaggaaca gcaaagtcaa
     2401 cctgattggt agccaatgta aattcacgac caatggcact ccaaacctgt acatctatct
     2461 taggtccata aaatgcactt tcatcaggca cttcaatata tggtatgttc gattctatca
     2521 atacacgtct gaccatatct tcggttttta accaaagttc aggctcatta acatatttct
     2581 ttcctaattt ttctggcgag tgcgtagaaa aacgcatgat gtatttctct attccgaaga
     2641 ttttaaaata ctttaagtac atgtcgttaa ctgccttaaa ctcagatgca aactgttctt
     2701 ctgtacagta aatatgggca tcgttcatct gaaggcaacg cacacgcatc aacccaaaca
     2761 attctcctga ttgttcataa cgatagcagg tgccatattc ggcaagtcta ataggtaaat
     2821 cgcgataact tgcattcaga ttcccaaata ttttatgatg gtgtgggcag ttcattggct
     2881 tgaggtagta cttttctcca tccatttcca ttggaggaaa catgctttct gcatagtaag
     2941 gaaggtgccc tgtagttaaa tacatacttt cctttgctaa atgaggtgta cgaacacgct
     3001 tgtagccggc ttcattttca gtacgcttag ccagattttc taattgctca attaaaacgc
     3061 caccattagg aagccataaa ggtaaacccg ggccaacttc atcatcaaac ataaagatat
     3121 caagctcttt gcctaatttt ctgtgatcgc gttttttggc ttcttccagc attgccagat
     3181 attcgtcaag ctctttctgc ttcggaaaag taattccgta aacacgggta agctgtttgt
     3241 tttttttcat cacccttcca gtaggcaccg gcaaggttca tcaacttaac tgctttaata
     3301 tgtgggccac gacataggtc agtaaaattg ccctgagtgt agaatgtaat gttaccgtct
     3361 tcgaggttct gaagcaggtc taatttatat tcatcatttt tttctgtgaa ataggctata
     3421 gcttcctgct ttggaacttc ttttctgata tagacattat tttttctggc aagctcaatc
     3481 attttgtctt caatcttctt aaagtcatcg cttgaaattt gtttgccgcc tannnnnnnn
     3541 nntttctaca ggaggcccaa cccagaattt gattcccgga aaataaaact ccaatgcttc
     3601 tgccattaaa tgtgccgatg aatgccacat cgtattttta ccatcctgac tatcccatgt
     3661 caataattcc aaagttgcat cactgttgat aggtattgaa aggtctgata cctgaccatt
     3721 aatttttgct gcaagtacat tgcgtgcaag cccttcgctg atgctcttgg ctatgtccat
     3781 tgcagtggtt ccatcaggat attctctgac gtttttgtcc ggaaaagtta ttttcattct
     3841 aaaaaaattt gcgcaaagat aattaactat ccatgtcgct aaaaaaaaca gatatttctg
     3901 tttctgcttt tctgcttaca tttgcccatg caccaaacca tactgatcat tgattttggc
     3961 tcacaataca cacaactaat tgccagaagg ctgcgtgagc ttaatgttta ttgcgagatt
     4021 catccttata accacctgcc tgaaatgagt gattccatta aaggagttat tttttccgga
     4081 agcccatttt ctgttcgcga cagtcaggcg cctcttatag acaccaaaag ttnnnnnnnn
     4141 nnnnnnnnnn nnnnnnncct gtgttgggat tgtgttatgg agcacagcta cttgcacatc
     4201 agcatcaggg tgaagtggtg gcttcaaaaa tcagagagta tgggcgagct attcttaaaa
     4261 ttaattttga tgatgaaatt tttaatgatg taagcgacaa cagtcaggtg tggatgtcgc
     4321 atgccgacac cattcgtagt gcaggtaatg actttgaagt catagcacnn nnnnnnnnnn
     4381 nnnnnnnnnn nnntggtttt cagtatcatc cggaagttta tcacagcact gatggaaaga
     4441 aaatgctagc caattttgtt tataatattt gtggctgccg tggcgactgg acaccatcat
     4501 cattcgttga tgaaacagtt gctcagttgc aagataagtt aaagaatgat aaagttattc
     4561 tagggctttc aggaggtgtt gattcaagtg tggctgcact gcttctgcat aaagctattg
     4621 gaaaaaatct gtattgtatt tttattgata atggcttgct gcgaaaaaat gaagcacagc
     4681 atgttatgga agtttttaag ccattaggat tgaatataaa atgtgtagat gctgccactg
     4741 tattttatga tgcattaaaa aacatatcag acccagagca aaaaagaaaa accattggca
     4801 gggtgtttat tgaagttttt gataaagaag ctcatgcaat ttctgatgta aagtggttgg
     4861 cacaaggaac catttatcct gatgttattg agtcagtatc tgtaaatggc ccttcggcaa
     4921 ccattaaatc gcatcacaat gttggaggac ttcccgagaa aatgaatctt aaaattgttg
     4981 agcctttgcg atcgcttttt aaagatgaag taaggaaggt gggcaaagaa atgggattaa
     5041 aagaagagtt attgaatcgc catccttttc caggtccggg gttaggaatc agaatattag
     5101 gtgaaataag tgcagaaaaa gtaaagatgc tgcaagaagc agatcatgtt tttatttctt
     5161 tattacaaaa acataacctc tatcagaaag tgtggcaggc aggagccatt ttcttacctg
     5221 ttaactcagt tggtgtaatg ggcgatgagc gtacttatga aattgcaatc tgtataagag
     5281 cagttaccag caccgatggt atgactgccg actgggcaca tctgccttat gagtttttaa
     5341 g
//
LOCUS       DAOOOI010000120         5244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2959_length_5244_cov_13.979572, whole genome shotgun sequence.
ACCESSION   DAOOOI010000120 DAOOOI010000000
VERSION     DAOOOI010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2959_length_5244_cov_13.979572"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..532)
                     /locus_tag="PKX59_10265"
     CDS             complement(35..532)
                     /locus_tag="PKX59_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002687749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOZ91284.1"
                     /translation="MFNTIEPEALLEQLTTQFAEKILLVEKHYDVSVAEVSHDVIKNV
                     IVFLKEQGFQFLTSCNGLHLPTAEKKFGMIYHLHDFKRNLRIRIKTFTQNDPPHFPTL
                     TDIFPAANWMEREAYDFFGFRFDGHPNLRRILNEDSMEGWPLRKEYPLEDQARFDKDD
                     RMFGR"
     gene            complement(551..1111)
                     /locus_tag="PKX59_10270"
     CDS             complement(551..1111)
                     /locus_tag="PKX59_10270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HOZ91285.1"
                     /translation="MSEKNLTNINQKVERLEGYEGPGFLATTLDKAVGLARKNSLWPL
                     PFATSCCGIEFMATMASHYDLARFGSERLSFSPRQADMLMVMGTIAKKMGPVLRQVYE
                     QMAEPKWVLSVGACASSGGIFDTYSVLQGIDRIIPVDVYVPGCPPRPEQILEGVMKIQ
                     ELVHNESLRRRNSPEYAELMASYGIK"
     gene            complement(1122..1493)
                     /locus_tag="PKX59_10275"
     CDS             complement(1122..1493)
                     /locus_tag="PKX59_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOZ91286.1"
                     /translation="MVLLSTPIDYLPIAIMFIVAMGFVLTTIFVTHLLGPKRKSEIKL
                     DTFECGIESKDNARLPFSIKYFLIAILFVLFDIEVIFMYPWAVSFLKLGMVGFLEMLL
                     FLALLLIGFIYVIRKGALKWE"
     gene            complement(1628..>5244)
                     /locus_tag="PKX59_10280"
     CDS             complement(1628..>5244)
                     /locus_tag="PKX59_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91287.1"
                     /translation="PIVSGVPPYFEDFVGCVNWTMYNGGLTNQWYIGNGTSAGGNRGM
                     YVSNNAGASNTYTVTASSTTHAMSNTVIDLTGGGACINLAFSYRCAGEGGYDWLDVFA
                     VPVSYVPTAGVAIPGGAVGGVLPNLSNPVRIAGPINGQSSYTTFTASLNALSGRQFRL
                     VFSWRNDGSVGNQPPAAVDNISIISTGGPLNDAPCTATFIPPLSNAGLYLPGSNICGS
                     NADEPSAPACWTNTGGVSQINTVWYRFRAPSSGCVRIRTERGSLYDTQIALYTRAPLV
                     GVVPCGSGNTLTYVSCNDNRVSCGSSQYYNSEITQSGLSAGFDYYIAVDGKNGDVGTF
                     TLFIMDGGAGCTNQYPPTPGADCSSPNIVCGSSISVPNPGYQGYGSLCDFSGGGGNCL
                     FSGDRGGAWYQVNIIGPGTLNFDIVPNDYNGSTATDYDFAVWKMAGSGALATCASIAN
                     SGTQTQGQVRCNYSALGVTGCYNVGNSPAIYPGFNGAYETGIPVVAGETYYIFVSNYS
                     NSTAGFALNFTYTTPSAAAVISSSVANGGTIIWSGTVSSSWTDPNNWGGCAIPSCSGG
                     TGETDALIPALYVNPPVITGTVSCRSLTINPGATLTVNGTGVLEICKDYKNNGNFVAQ
                     AGSRVIFDGPAIGTYTGNQNIDGNLTGTNAFHHLDIFKASMANQVLANQDFDVRGNLK
                     VGYNSGTSATNRLTNFLTQPSGNGKYVKVGGDFLVYQDAGGTAAFVTGGGSVLEFTGS
                     ANQNYMNRNVLGSVFVNQSAPSTVTVQPHGGSAFGHMTLSTSGTLTFATGKIVVNGSV
                     NNTGVPATNKGYVEMQNPLPGTITGMNNTSYISGTGNAPAFYTLKKATTGAVGVYDLP
                     VGTITKYSNLKLDAKTVLSPNPSYFLVGFDNTVPATNTAFTGSAIDECSVKYHSSGVN
                     PLNNGLWRIITGSSAAGTAGVLDISLTENGYGNGANGQTVMYNKLGTPATAADWLLNP
                     FPASACISGNSLPGPVSRTNMQWATIKPASVTTPLMFGTAQSPTPLPVEYLALTAKGV
                     QNTIKVEWSTASEKNNKGYEVQRSTDGQMFENIGWVNGHGTTNLMNMYQYTDISVSKG
                     ITYFYRLKQIDYDGMFDISKKVAASISDNNLSFNVSPNPYSEQTNVVYQLDQTSEVTL
                     EVFNKLGQKVTTLHKGIQEPGTYQYPFNAKQYGFSAGVYTVKMTINSQIFTRLLFENN
                     "
BASE COUNT         1606 a         1087 c          942 g         1609 t
ORIGIN      
        1 tgtttgattt cttctttcat tttttacaag taaattaacg tccaaacatt ctatcgtctt
       61 tatcaaatcg tgcttggtct tcaagcggat attcttttcg aagcggccag ccttccatac
      121 tgtcttcgtt caaaattctg cgaaggtttg ggtggccatc aaaacgaaac ccgaaaaaat
      181 cataagcctc gcgctccatc caattagctg caggaaaaat atctgtcagc gttggaaaat
      241 gtggagggtc gttttgtgta aatgttttaa ttctgatgcg gaggttgcgt ttaaaatcat
      301 gcagatgata aatcattccg aatttttttt ctgcagtagg aagatgtaaa ccattgcagg
      361 aagtcaaaaa ctgaaaacct tgctccttta gaaatacaat tacatttttt atcacatcgt
      421 gtgatacttc cgcaacagaa acatcgtaat gtttttcaac taataaaatt ttttctgcaa
      481 actgtgttgt aagttgctca agtaatgctt caggttcaat cgtgttaaac atctttacaa
      541 tttttattca ctatttaatt ccgtaacttg ccattagctc tgcatattca ggagagtttc
      601 ttctgcgtaa tgattcgttg tgtaccaatt cctgaatttt catcacacct tctaaaattt
      661 gttcggggcg tggtggacaa ccgggcacat aaacatcaac aggaataatt ctgtcaatac
      721 cctgtagtac actgtaggtg tcaaaaatac caccggaact tgcacatgca ccaacagaaa
      781 gaacccattt tggttcggcc atttgttcgt aaacttggcg taatacaggt cccatttttt
      841 ttgcaatggt tcccattacc atcagcatat ctgcctggcg tggagaaaaa cttaaacgct
      901 cagagccaaa tcttgcaagg tcataatgcg aagccattgt agccataaat tcaatgccac
      961 agcatgatgt tgcaaagggc agtggccaaa gcgagttttt tcgtgcaaga ccaactgctt
     1021 tatctaatgt ggtagctaaa aatccgggcc cttcatagcc ttctaaacgt tctacctttt
     1081 ggtttatatt ggttaaattt ttttcgctca taacaatttc tttattccca ttttaatgcg
     1141 cctttacgga ttacataaat aaatcctatc agcaataatg caagaaagag taacatttca
     1201 agaaagccta ccataccaag ttttaagaaa ctaacagccc atgggtacat aaaaattacc
     1261 tctatgtcaa acaatacaaa aagaatagca atcaaaaaat atttgatgga gaatggcaga
     1321 cgtgcattat cttttgattc tataccacat tcaaaagtgt cgagtttaat ttctgatttt
     1381 ctttttggtc ccaacaagtg agtaacaaag atggtggtta gtacaaaccc cattgctaca
     1441 ataaacatga tggcaattgg cagatagtct attggtgtgc ttaaaagtac cataaaaata
     1501 tttattcttt ggcaaaatta gcctttatat gatacaatag caaagcaaag tattttgttt
     1561 tgtaatgccg taaaaatcaa taaggaggcc caaagcctcc ttattgatta atttgtaaat
     1621 tttaaaatta gttgttttcg aaaagcagcc ttgtaaatat ctggctatta atggtcattt
     1681 taacggtata aacacctgca gaaaatccat attgcttagc gttgaatgga tattgatatg
     1741 ttccgggttc ctgaattcct ttatgcagtg tagttacttt ttgtcccaac ttattaaaca
     1801 cttccaatgt tacttcagaa gtctgatcta actgatacac cacattggtt tgttctgaat
     1861 aaggattagg actaacatta aatgataagt tgttatcact gattgatgca gcaacttttt
     1921 tactgatatc aaacatacca tcataatcaa tctgcttcag acgatagaaa taggttatac
     1981 cttttgagac actaatatca gtatattgat acatattcat caggttagtc gtaccgtgac
     2041 catttaccca gcctatattc tcaaacattt gaccgtctgt acttcgttgt acttcataac
     2101 ctttgttgtt cttctcacta gcagttgacc attctacttt aatggtgttt tgaacacctt
     2161 tggctgtaag agcaaggtat tcaaccggta gtggagtagg tgactgtgca gtaccaaaca
     2221 tcaggggtgt tgttactgat gcaggcttaa ttgttgccca ttgcatgttg gttcttgaaa
     2281 ctggtccggg taaagagttt ccacttatac aagcacttgc cggaaatgga tttagtaacc
     2341 aatctgcagc agttgcaggg gtacctaatt tgttatacat tacagtttgt ccgttggcac
     2401 cattaccata tccattttct gttagtgaga tatctaaaac gcctgcagtt ccagcagcag
     2461 aactaccggt tataattctc cacaatccat tattcaatgg attaactcct gatgagtggt
     2521 atttaactga acactcgtca attgcacttc cggtgaaagc agtgtttgtt gcaggtactg
     2581 tattatcgaa tccaactagg aaataggatg ggttgggaga taaaactgtt ttagcatcaa
     2641 gtttaaggtt agaatatttt gttattgtac ctaccggtaa atcataaaca ccaacagcac
     2701 cggttgtagc ttttttcagc gtataaaatg ctggtgcatt tccggttcct gaaatatagc
     2761 tggtattatt catacctgtt atcgtgcctg gtaatggatt ctgcatttca acataacctt
     2821 tattagttgc aggcacaccg gtattattta ctgaaccatt gacaacaatt ttgcctgttg
     2881 caaaagttaa tgttccggat gtactcaaag tcatgtgacc aaaagctgat ccaccatgtg
     2941 gttgaactgt aactgtagaa ggggcagatt gattaacaaa aacactacct aaaacatttc
     3001 tgttcatata gttttgattt gccgatccgg taaactctaa aaccgaacca ccaccggtaa
     3061 caaaggctgc tgtaccaccg gcatcctgat agacgagaaa atcaccacct acctttacat
     3121 attttccatt accactgggt tgcgttaaga aatttgtcaa acggtttgta gcagaagtac
     3181 cggaattgta accaactttt aaattaccac gaacatcaaa atcctgattg gctaatacct
     3241 gattggccat tgaagcttta aaaatatcta aatgatgaaa agcattagtt cctgttaggt
     3301 ttccatctat attctgatta cctgtataag tacctattgc aggtccatca aaaattactc
     3361 ttgagccagc ctgagctaca aaatttccat tgtttttata gtctttacat atttcaagta
     3421 ctcctgttcc gttcactgtt aaagtagcac ccggattaat agttaaacta cgacaactaa
     3481 cagttccggt aatgactgga ggattaacat acaatgcagg aattaatgca tcagtttcac
     3541 ctgttccacc actacaactt ggaatggcac agccgcccca gttgtttgga tctgtccaag
     3601 atgagcttac ggttcctgac catataatgg taccaccatt ggcaacagaa gatgaaatta
     3661 ctgcagcggc acttggtgtt gtataagtaa agtttaatgc aaaccctgca gtactgttac
     3721 tgtaattact aacaaaaata tagtaggttt cacctgctac tacaggtatt cctgtctcat
     3781 aagcaccatt aaagccgggg taaatagcag gagaattacc tacattataa catcctgtta
     3841 cacccaaagc actataatta cacctaacct gaccctgtgt ttgtgttcct gaatttgcaa
     3901 ttgatgcaca agttgcaagt gctcctgaac ctgccatttt ccaaaccgca aagtcatagt
     3961 cagttgcagt tgagccgtta tagtcattag gtacaatatc aaaatttaat gttcctggac
     4021 caatgatatt aacctgatac caggcaccgc ctctgtcacc tgaaaacaga caattgcctc
     4081 ctccacctga aaaatcgcaa agtgagccat agccctgata gccagggtta ggaacagaaa
     4141 tagatgatcc acaaacaata tttggtgaag agcaatctgc acccggagtt ggtggatatt
     4201 gattggtaca tcctgcacca ccatccataa tgaataaagt aaacgttcca acatcaccat
     4261 ttttaccatc tacggctatg taataatcga agccggccga aagtccggac tgtgttattt
     4321 ctgagttgta atattggctc gaaccacatg atactctgtt atcattacaa gaaacataag
     4381 ttaatgtgtt tccggatccg caaggtacta cacctactaa aggtgctctt gtataaagag
     4441 caatttgagt atcgtataat gaacctcttt ctgtacgtat tcttacacaa ccactacttg
     4501 gtgcacggaa acgataccaa acagtattta tttgactcac acctcctgta tttgtccaac
     4561 aagcaggtgc actaggctca tctgcattac taccacaaat gttactaccc ggaaggtata
     4621 agcctgcatt agataatgga ggaataaaag ttgcagtaca tggtgcatca tttaacggac
     4681 ctcctgttga tattatgcta atattgtcaa ctgctgccgg aggttgattt ccaacagagc
     4741 catcgtttct ccagctaaat accaatctga attgccttcc tgataatgca ttgagagaag
     4801 cagtaaaggt tgtataagac gattgtccat ttattggacc tgcaatgcga accggattag
     4861 ataaattggg taatactcca ccaactgcac caccgggtat agctacaccg gcagttggaa
     4921 cataagaaac cggtacggca aatacatcaa gccaatcata accaccttca ccggcacatc
     4981 tgtaagaaaa agctaagtta atacatgcac caccaccagt aagatctatc acagtattgc
     5041 tcattgcatg agtcgtggaa cttgcagtaa cagtatatgt gttacttgcg cctgcattat
     5101 ttgaaacata catacctctg ttaccacctg cactggttcc atttccaata taccattgat
     5161 tggttagacc accattatac atagtccaat ttacacatcc tacaaaatct tcaaaatacg
     5221 gaggtacacc actcacaatt gggt
//
LOCUS       DAOOOI010000121         5211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_2983_length_5211_cov_8.598720, whole genome shotgun sequence.
ACCESSION   DAOOOI010000121 DAOOOI010000000
VERSION     DAOOOI010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2983_length_5211_cov_8.598720"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PKX59_10285"
     CDS             complement(<1..296)
                     /locus_tag="PKX59_10285"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HOZ91288.1"
                     /translation="MAKKVIKFDYSSEFDFLLAGIVCPYKDYRLCFELNRALKLSFTR
                     MKDHTLHLGKPGAKAAFSFYKSINRFGEEFYVLGNKSVQATLIPEKPAFDFFMIV"
     gene            358..807
                     /locus_tag="PKX59_10290"
     CDS             358..807
                     /locus_tag="PKX59_10290"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOZ91289.1"
                     /translation="MSIIQQGDDYFMQEALKEARKAFDSDEVPVGAVIVHAGRIIARA
                     HNMTEKLNDVTAHAEMICFTSAAAFLNSKYLTDCVLYVTLEPCVMCAGASFWTQIPQI
                     VFGAADAKRGYQRCETNLLHPSTKIKGGVLEDACSNLLKEFFRKKRI"
     gene            complement(804..1193)
                     /locus_tag="PKX59_10295"
     CDS             complement(804..1193)
                     /locus_tag="PKX59_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ91290.1"
                     /translation="MANKYHSVLVVDDSEAEQVMIKQIITSEPFCDQLNVYADARKAL
                     DFIKATPIDQLPEIIFLDIVMPEMDGFQFLNEFEKLSESVRQKCKIVLLSSSDSFKDL
                     NRANKCRYVRRFLNKPLTNSMLQAINF"
     assembly_gap    1237..1246
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1247..1721)
                     /locus_tag="PKX59_10300"
     CDS             complement(<1247..1721)
                     /locus_tag="PKX59_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOZ91291.1"
                     /translation="MIRTVTAIPEGNFNSVDFSEVIRQSIDRLRYTEGIDKVNFDIKV
                     DMKAPFAGNNKLLEVVFNNLISNGIKYRKITGDPSSMRLHVYCDEQKAIINIQDNGLG
                     IAPDEVGQIFNLFQKEKQPEPGRGIGLYMAKEIIEKLGGNITVESVLNTGTAIIND"
     gene            complement(1718..2071)
                     /locus_tag="PKX59_10305"
     CDS             complement(1718..2071)
                     /locus_tag="PKX59_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91292.1"
                     /translation="MAEHKTSTSKYENNMFPVIKLDYSGKIIYHNVAAMPILSYWQCK
                     LNAPVNNQVKAIYPEIFNVKTPVPHDASVQYLEYLFHFSVIPYPEAGYIGIYGHCIEE
                     ALVAVTNSTEQHHSK"
     gene            2350..2766
                     /locus_tag="PKX59_10310"
     CDS             2350..2766
                     /locus_tag="PKX59_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HOZ91293.1"
                     /translation="MPYPEQLCAPMRQELTNVGFEELKTTSDVESSLPNSKGTVLLVV
                     NSVCGCAAGTARPGVTLSLRGDKKPGKLVTVFAGQDVEATAQARKYTLPYPPSSPCIA
                     LFKDGELVHFVERHHIEGRSAEMIAENLKAAYEEFC"
     gene            2881..4419
                     /locus_tag="PKX59_10315"
     CDS             2881..4419
                     /locus_tag="PKX59_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOZ91294.1"
                     /translation="MLVKTYGCAVYGINATPITVEVNVDQGTKFFHVGLPDNAVKESH
                     QRIEAALKNTGFKMPGKKVVVNMAPADIRKEGSAYDLTIAMGILSASQQIVAENIEQY
                     IIMGELSLDGSLQPIKGALPIAIEARKHGFKGFFLPQQNAREAAIVDSLDVYGISNIK
                     EVIDFFTGEGTLQPVVINTRDEFYSAQTKSEFDFCDVKGQENIKRSLEIAAAGGHNVI
                     LIGPPGAGKTMLAKRLPTILPPLTLHEALETTKIHSVAGKMGKQASLISIRPFRSPHH
                     TISDVALVGGGGIPQPGEISLAHNGVLFLDELPEFKRTVLEVMRQPLEERRVTISRAK
                     FSVEYPASFMLVASMNPCPCGFFNHPEKECVCAPGIVQRYLSKISGPLLDRIDLHVEV
                     TPVSFSELSKKRVSEKSEDIRKRVVEARVIQEERYKEQEGVYCNAQMSSKMLHDICRI
                     SDEGNQLLKTAMEKLGLSARAYDRILKVSRTIADLAASADIKTEHLAEAIHFRSLDRE
                     GWAG"
     gene            4583..4656
                     /locus_tag="PKX59_10320"
     tRNA            4583..4656
                     /locus_tag="PKX59_10320"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4617..4619,aa:Asn,seq:gtt)
     gene            4726..5130
                     /locus_tag="PKX59_10325"
     CDS             4726..5130
                     /locus_tag="PKX59_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOZ91295.1"
                     /translation="MTTINHVPIVIGTGSTPIAIGAGGGAKPKVKPSAFLFITMVYSV
                     YILYSIKLGVHYIGTTNDVARRLEQHNKQEFADSFSAKGIPWELKFVIGELNSTQAFA
                     IEKHIKKMKSVVYIENLIRFPEIVEKLKHKYK"
BASE COUNT         1639 a          914 c         1075 g         1573 t
ORIGIN      
        1 acaatcataa aaaagtcgaa ggcaggcttt tcgggaataa gtgtggcctg aacactttta
       61 tttccaagta cataaaactc ttcgccaaaa cgattgatgg atttgtaaaa agaaaaggct
      121 gcttttgcgc ccggcttgcc aagatgcaag gtgtgatctt tcattctggt gaaagaaagt
      181 tttaatgcac ggtttagctc aaagcaaaga cgatagtctt tatacggaca tacaattccg
      241 gcaagcagaa agtcaaactc actgctgtaa tcaaacttta taaccttctt tgccaagaga
      301 aacagattgt ttatttaaaa cggatatttt cgctgctaaa ttagattaat ttgcagtatg
      361 agcataattc agcaaggcga tgattatttt atgcaggaag cattaaagga agcaagaaaa
      421 gcttttgaca gtgatgaagt tcctgtgggt gcagtaattg ttcatgcagg acgaattatt
      481 gcccgcgcac ataatatgac agaaaagctg aatgatgtta cggcacatgc agaaatgatt
      541 tgctttactt cagcagcagc atttttaaac tcaaaatacc tgaccgattg tgtactttat
      601 gtaacgcttg aaccttgtgt gatgtgtgcc ggagcatcct tttggacaca gatacctcag
      661 attgtttttg gtgctgccga tgccaaacga ggctatcagc gttgcgaaac caatctgctg
      721 catccttcaa caaaaataaa aggaggcgta cttgaagatg cctgtagcaa cttactgaaa
      781 gaattttttc gaaaaaaaag aatctagaaa ttaattgcct gcagcatgga gttggttaat
      841 ggtttgttta aaaaccttct cacataacgg catttgttgg cacgattcag atctttaaaa
      901 ctgtctgaac tcgacaataa aacaatcttg catttctgac gcaccgattc agaaagtttc
      961 tcgaactcat tcagaaactg aaagccatcc atttcaggca taactatatc taagaaaatt
     1021 atttcgggca actgatctat aggtgttgct ttaataaaat caagtgcttt acgggcatca
     1081 gcataaacat ttagctgatc gcaaaaaggc tccgatgtaa tgatttgttt tatcatcacc
     1141 tgttcggctt cactatcatc tacaactaaa acagagtggt atttattggc cataagctta
     1201 ttcaaaatag ttatttaatg gtttggtgtt taatttnnnn nnnnnntatc attgatgatt
     1261 gctgttccgg tatttaaaac agattcaacg gtgatgtttc cacctagttt ttcaataatt
     1321 tctttggcca tatacaagcc aattcctctt ccgggttcag gctgtttttc tttctgaaac
     1381 agattaaaaa tttgtcctac ttcatcaggt gcaattccca gtccgttatc ctgaatattg
     1441 atgatggctt tttgttcatc acaataaaca tgtaaccgca tagaagatgg gtcacccgta
     1501 atttttctgt atttaatgcc gtttgaaatt aaattattga aaacaacttc aagcaattta
     1561 ttgttgccag caaaaggggc tttcatatct actttaatgt caaaatttac tttatcaatt
     1621 ccttctgtat agcgcaacct gtctattgac tgacgaataa cttcgctaaa gtcaacagaa
     1681 ttaaaattgc cttcgggaat tgcagtaacc gttctgatca ttttgaatga tgttgctctg
     1741 ttgaatttgt tacagcaact aatgcttctt caatacagtg tccataaatt ccgatatatc
     1801 ctgcttcggg atatggaatt acagagaaat gaaataggta ttcaagatat tgcactgaag
     1861 catcatgtgg cacaggcgtt tttacattaa aaatttcagg ataaatagct tttacctgat
     1921 tgtttacagg agcatttagt ttgcattgcc aataacttag tataggcatt gccgctacat
     1981 tgtgataaat aatttttccg ctgtaatcta attttattac aggaaacatg ttgttttcgt
     2041 actttgaagt agaggttttg tgttctgcca ttgtggagat ttctttaaat taaacagtag
     2101 tttaacgaca gtttttcaga aatgcttcac tgctatgggt ggttatgctt acagatgtaa
     2161 gattttgctt aaattattca actttttatt caattaaaat tgaattgccc atgagaaaag
     2221 aaaaaattca ggaattctgt ggtgtcaaaa aatattgtaa gaaacttaaa caatgtgctt
     2281 taaaatctgt tatctgaact gaataacaaa taagagtatt tatatacttt tgcaaaaaaa
     2341 tatttaaaaa tgccatatcc ggaacaatta tgcgcaccta tgcgccagga attaaccaat
     2401 gtaggttttg aggaattaaa aaccacttca gatgttgaat catcattacc aaacagcaaa
     2461 ggaacagtgc tgttggttgt aaactcagta tgcggatgcg ctgcaggaac ggcacgtccg
     2521 ggagtaacac tttctttaag aggtgataaa aaacccggaa aattggttac agtatttgca
     2581 ggacaagatg ttgaagcaac ggcacaagcc agaaaataca ccctgccata tccgccatct
     2641 tcaccatgca ttgcattgtt taaagacggt gagttggtac attttgtaga acgtcatcat
     2701 attgaaggcc gctcggctga aatgattgca gagaatctga aagctgctta cgaagaattt
     2761 tgttagccac attttccgca gaaaaaaaac ctttgcggat gatgttttag gtttttaaag
     2821 ttattaatgg ctatttttgt ttatcgaata gaaaaacaga attagccatt attattttat
     2881 atgcttgtta aaacttacgg ctgtgcagtc tatggaatca atgccacacc aataacggta
     2941 gaagtaaatg ttgatcaggg aaccaaattt tttcatgttg gcttacccga taatgctgta
     3001 aaagaaagcc atcagcgaat tgaagcagca ttgaaaaata caggctttaa aatgcccgga
     3061 aaaaaggtag tagtaaacat ggccccagcg gatattcgca aagaaggctc ggcttatgat
     3121 ttaactattg ctatgggaat actttctgca agtcagcaga tagttgccga aaacattgag
     3181 caatatatta tcatgggtga gctttcgctc gatggcagcc tgcagcctat aaaaggggca
     3241 ttgccaatag ctattgaagc gcgcaaacat ggattcaaag gtttttttct gccgcaacaa
     3301 aatgcacgcg aggcggccat tgtagattcg ctcgatgtgt atggtatcag taacattaaa
     3361 gaggttatag attttttcac cggtgaaggc actttgcaac ccgttgtgat caatacacgc
     3421 gatgagtttt attctgctca gacaaaaagt gagtttgatt tctgtgatgt taaaggtcag
     3481 gaaaacatta aacgctctct ggaaattgca gcagcaggag ggcacaatgt aatattgatt
     3541 ggtccgcccg gtgcgggtaa gaccatgctg gcaaagcgac ttcctaccat attgccacca
     3601 ttgacattac atgaggcatt agaaacaaca aaaatccatt ccgttgccgg caaaatgggt
     3661 aaacaggcat cattaatttc tatacgtccg ttccggtcac ctcatcatac cataagcgat
     3721 gttgcattag ttggcggagg cggaattcca cagccaggag aaatatcact agcacataat
     3781 ggcgtattgt ttttagacga gttgcctgaa tttaaacgaa cagtacttga agtgatgcgt
     3841 cagccgctgg aggaacgcag ggtgaccatt tccagagcta aattttctgt agaataccct
     3901 gccagtttta tgcttgtagc ttctatgaac ccctgtcctt gtggattttt taatcatccg
     3961 gaaaaagaat gtgtttgtgc tccgggtatt gtgcagcgct atctcagtaa aatttcaggg
     4021 ccattgctcg acaggattga cctacatgtt gaagttacac cggtttcttt ttcagagcta
     4081 tcgaaaaaac gtgtctccga aaaaagcgaa gatatcagaa aaagggttgt tgaagcacgg
     4141 gtaattcagg aagaaagata taaagagcag gaaggcgttt actgtaatgc acagatgagc
     4201 agtaagatgc tgcacgacat ctgtcgtatt tctgatgagg gtaatcaact gctaaagact
     4261 gcaatggaga aattaggtct ttcggcacga gcctatgaca gaatattaaa agtatctcga
     4321 actatagccg atttagctgc cagtgctgat ataaaaaccg agcatttggc cgaggcaata
     4381 cacttcagaa gtttagatag agaaggctgg gcaggataaa aaatagggtt aaaatttttt
     4441 tttaaccaaa aaaagctatt tttgccgtcc ctttacaaac gtgttcgtga ttacacgtgc
     4501 tttagttccg attgttatcg gaatggttag agttaagaaa aacatttttc tgtcttaggg
     4561 cagaaattct taagaggtaa attcctcctt agctcagctg gttagagcac atgactgtta
     4621 atcatggggt ccctggttcg agcccaggag ggggagcaaa gccgaaggta aagccttcgg
     4681 cttttttaat tttgatttaa aaaaatcagt aagtactttg aagaaatgac aactattaat
     4741 catgttccaa tagtaatcgg aactggttcg accccgatag ctatcggggc aggaggggga
     4801 gcaaagccga aggtaaagcc ttcggctttt ttatttatta ctatggttta ttctgtttat
     4861 attctttact ctataaaact tggtgtacat tatattggaa caactaatga tgttgcaaga
     4921 agattagaac agcataacaa gcaagagttt gcagactcgt tttcagcaaa agggattcca
     4981 tgggaattaa aatttgttat tggagaatta aatagtacgc aagcttttgc aattgaaaaa
     5041 catattaaga agatgaaatc tgttgtgtac attgaaaatc tgattcggtt tccggagatt
     5101 gttgaaaagc ttaaacataa atataaataa tggttcgacc ccgatagcta tcgggacagg
     5161 agggggagca aagccgaagg taaagccttc ggctttttta tttattacta t
//
LOCUS       DAOOOI010000122         5095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3059_length_5095_cov_7.334921, whole genome shotgun sequence.
ACCESSION   DAOOOI010000122 DAOOOI010000000
VERSION     DAOOOI010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3059_length_5095_cov_7.334921"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PKX59_10330"
     CDS             <1..232
                     /locus_tag="PKX59_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOZ91296.1"
                     /translation="ETMAVLMVTGNAAVIPHSILQPVRTIPATIAAELGEAPFGGLHF
                     KALFFLGCVLFLITMIINFIVELISPKRRIER"
     gene            234..1100
                     /gene="pstA"
                     /locus_tag="PKX59_10335"
     CDS             234..1100
                     /gene="pstA"
                     /locus_tag="PKX59_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOZ91297.1"
                     /translation="MNYEFKKRLKQNIAFWLLRIFTFSVLIILFVILGFIVKKGIGVI
                     SWEFLTEMPKDGMTSGGIFPAIVGTLLLVLGSMLVAFPIGIMSGICMSEYVKDGFIKR
                     FVTVMTNNLAGIPSIVFGLFGMEFFVNELKWGDSIIAGSFTLGLLAIPIVIRITEESL
                     KSVSKTFREASMALGATKLQTTYKVVLPIAMPNIVTGLILSLSRGSGETAPILFTVAA
                     YFLPKLPNSIFDQVMALPYHLYVISTSGTNIEASRHMAYGTALVLIIIVLMFNLLANW
                     LRKYLSRKVKMN"
     gene            1184..1966
                     /gene="pstB"
                     /locus_tag="PKX59_10340"
                     /pseudo
     CDS             1184..1966
                     /gene="pstB"
                     /locus_tag="PKX59_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
     gene            2024..2713
                     /gene="phoU"
                     /locus_tag="PKX59_10345"
     CDS             2024..2713
                     /gene="phoU"
                     /locus_tag="PKX59_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOZ91298.1"
                     /translation="MTHLDVEIARLKKDMLDLFGMVKLQLEKSKQALIHLDKDLAREV
                     MVNEKRVNAVEIKLDRDCENIIALFNPVAIDLRFVLACLKIINNLERTGDIADGIARY
                     VLNIKLEPAQKLIDTTRLVEMYDISCNMLDVVMTAFAEENTNLARSVFGMDDKLDEIF
                     HQSNQKVGEFIKENPDRINQSLYVFSASSKLERVGDQCKNIAEELIFFVEAKVLKHSV
                     KSTSKSENQKT"
     gene            complement(2722..2943)
                     /locus_tag="PKX59_10350"
     CDS             complement(2722..2943)
                     /locus_tag="PKX59_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91299.1"
                     /translation="MYESSTTHDLILYYFNEVELADTVLIQHEIDHNPETEENFQEIV
                     QVMDHIDNCMVNPSASVIKNILNYSHSLK"
     gene            complement(3009..3593)
                     /locus_tag="PKX59_10355"
     CDS             complement(3009..3593)
                     /locus_tag="PKX59_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOZ91300.1"
                     /translation="MQQPVLNDRELVEMYQQGNEDCLTVLIERHQKRIFSAILFLVNN
                     RELAEDIFQDTFVKVINTLKKGSYNEEGKFLPWVLRISHNLVIDHFRRNQRMPMVHDK
                     EDYSIIESLQLMDDNVQDKIVKEQIYKEVRLLVEELPYEQKEVIILRHFADMSFKEIA
                     DATGVSINTSLGRMRYALINLRKVIEKKKLVLAA"
     assembly_gap    3804..3885
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3886..4304)
                     /locus_tag="PKX59_10360"
                     /pseudo
     CDS             complement(3886..4304)
                     /locus_tag="PKX59_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
     gene            4407..>5095
                     /locus_tag="PKX59_10365"
     CDS             4407..>5095
                     /locus_tag="PKX59_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOZ91301.1"
                     /translation="MIPKFEQHSIEQLKNLLSEPKRIVITTHYKPDGDAIGSLLGLYN
                     FLVAKKHKVKAVVPSDYPASISFMKGNQKVLDYEAKPRNVAKAFSEAEIIFCLDFNES
                     KRVEAMEEVMMSNKAFKVVIDHHLYPKIECDILFSFPKSCATSELIFHFLYALDNKYK
                     LKPEVAECIYTGIMTDTGSFRFSSMSADTHRVLAALLDTGMNHVKIHETISDNFSEER
                     TRFLGYCLKDK"
BASE COUNT         1595 a          812 c          969 g         1637 t
ORIGIN      
        1 agaaacaatg gcagtgctta tggttaccgg caatgctgcc gtaattccac attctatttt
       61 acagcctgtg cgtacaattc ctgcaactat tgctgctgaa ttgggcgaag caccttttgg
      121 aggattacat tttaaagcat tgttcttttt aggatgtgtg ctctttctga ttacaatgat
      181 tataaatttt attgttgaat taatttcacc aaaacgcaga atcgaacgat aaaatgaact
      241 acgaatttaa aaaacgactt aaacagaaca tagctttttg gctgttgcgt atattcacct
      301 tttctgtttt aattatcctg tttgtaatac ttgggtttat tgttaaaaaa gggataggtg
      361 taatcagctg ggagtttctt acagagatgc caaaagatgg aatgacaagt ggtggaattt
      421 ttcctgcaat agttggtaca ctattacttg ttttaggcag tatgcttgtg gcatttccga
      481 ttggtatcat gtcaggcatt tgcatgagcg aatatgttaa agatggtttt ataaaacgtt
      541 ttgttaccgt aatgacaaat aaccttgccg gcattccttc aattgtgttt ggattgtttg
      601 gtatggaatt ttttgttaat gagctaaagt ggggcgattc gattattgcc ggatcgttta
      661 cattaggact tcttgcaatt cctattgtca taagaataac agaagagtct ttgaaatctg
      721 tatcaaaaac ttttcgtgag gccagtatgg cattaggcgc aacaaagctt cagactactt
      781 ataaggtggt gttgccaatt gctatgccca acattgttac gggattgatt ttaagtttga
      841 gcagggggtc aggagaaact gcgccaatac tgtttacggt ggcagcttat tttctgccca
      901 aacttccaaa cagtattttc gatcaggtaa tggcattgcc ctatcacctc tatgttattt
      961 caacaagcgg aacaaatatt gaagcatcaa ggcacatggc ctatggcact gcattggtat
     1021 taataattat tgtgctcatg tttaatctgc ttgcaaactg gctaagaaag tatcttagtc
     1081 gtaaggttaa aatgaattaa tagtgtatca aacaaaataa agaaaaatga ttgtaatgga
     1141 aaagcaattt aaggataaag agtcaataga agatgtaatg caaatgcagg agccttcaaa
     1201 gcaaaatcgg acggtaaaga ttgaaagtag aaatatggaa ctctactacg gagaattcca
     1261 tgccctaaaa ggtatcaata ttgcaattaa gaccaactct gttgctgcac tcatcgggcc
     1321 ctccggatgt gggaaatcta cttttctgcg ctcgctaaac agaatgaatg accttattga
     1381 tggcgttaaa atcagtggtc aggtattgat tgacgggaaa gatatttaca atagtaaagc
     1441 gcttagaatt gaagaactgc gtaagaaagt tgggatggta tttcagaaac ccaatccatt
     1501 tcctaaatca atttttgaaa atgtagcgta tggtttacgt gttaatggta tctcgcataa
     1561 gtcttttatt gaagagcgtg ttgaacgctc tttaaagcaa gctgcactgt gggatgaggt
     1621 aaaagataaa cttaaaaaat ctgcatttga attatcgggc ggacagcaac agcgtttatg
     1681 tattgctctg tcattggctt ttgagtcttc ttttttatta atggacgaac ctgcttctgg
     1741 actcgatact atttctacat caaaaattga agagcttatt tatgaattat agtcagatta
     1801 tactattgtt attgtaacac ataacatgca gcaagcaggt cgtgttagtg atatgactgc
     1861 attcttttat ctgggtgaat tggtagaata tgattctaca aagaaaattt ttaccaatcc
     1921 tgaagacctg agaactcaaa attatattac agggcgattt ggataattaa ttctgttcct
     1981 tattcagtaa aattttaaat aaaagtaaaa aacaaaactt agtatgacac atttagatgt
     2041 tgaaatagca agacttaaga aagatatgct cgatctgttt ggtatggtga agctacaatt
     2101 ggaaaaatct aaacaggcat taattcattt agataaagat ttggcacgag aagtaatggt
     2161 aaatgaaaaa cgtgtcaatg cagttgaaat aaaacttgat cgcgattgtg agaatattat
     2221 agcgctattc aatcctgttg caatagatct tcgatttgtc ttggcttgtc tgaaaataat
     2281 caacaacctt gaacgaacag gtgatattgc agatggtata gcccgttatg tattgaatat
     2341 taaacttgag cctgcacaaa aattaattga tacaacaaga ttggtagaga tgtatgatat
     2401 atcctgcaat atgcttgatg ttgtgatgac ggcattcgca gaagaaaata caaacttagc
     2461 cagaagtgtt tttggaatgg atgataaact tgatgagatt tttcaccagt caaatcaaaa
     2521 agtaggtgag tttatcaaag aaaatcctga cagaataaat caaagtctct atgttttttc
     2581 tgccagcagc aagttggagc gcgttggcga tcaatgcaaa aatattgcag aagagctaat
     2641 cttttttgtt gaagcaaaag tgctgaagca ctctgtgaaa tcaacatcaa aatcagaaaa
     2701 tcagaaaact tgatattaga attacttcaa tgaatgtgag tagttcagaa tgtttttgat
     2761 aacagatgca gagggattaa ccatgcagtt gtcaatatga tccattacct gaacaatttc
     2821 ctgaaagttt tcttctgttt caggattgtg atcaatttca tgttgaatca acacagtgtc
     2881 agctaattca acttcgttaa agtagtaaag aattaaatcg tgtgtagtag aactttcgta
     2941 cataggcgtg aaacttattt acacctataa cgcaacgcaa tccggaatat tttatgattg
     3001 cctgaaaatt atgctgccag caccaatttt ttcttctcaa tcacttttct aaggttgata
     3061 agtgcatagc gcatccttcc taaagaagtg ttgatactca cacctgtagc atcggcaatt
     3121 tctttaaaac tcatgtctgc aaagtgtctc agaatgatga cttctttttg ctcgtaaggt
     3181 aattcttcaa ccaacagacg cacttccttg taaatctgtt ctttgacaat tttatcctga
     3241 acgttatcgt ccatcagttg caatgattca atgattgaat aatcctcctt gtcatgaacc
     3301 attggcatac gctgattgcg tctgaaatga tcaataacta agttgtggct gatgcgaaga
     3361 acccaaggta aaaatttacc ttcttcgtta tagcttcctt tttttaaggt gttgattacc
     3421 ttaacaaagg tgtcctggaa aatatcttcg gctaattctc tattattgac aagaaagaga
     3481 atggcagaaa atattctttt ctggtgtcgt tcaatcagaa cggttaagca gtcttcgtta
     3541 ccttgttggt acatttcgac cagttctcgg tcgtttaaaa caggctgttg catagttaac
     3601 tcttttttgg aaataagtgt tgaagtagag ttttatgcta tagttttcct cctgtttgaa
     3661 atgttattgt gagatgtttt gagatataga acgtgttatt tttaataatg ttgtatagct
     3721 cagaaaatat ttttattaat tattcagtaa acataaagaa aaaatcctgc caattcaact
     3781 ttgaggtaat aagtgacagg attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttaaa atctttcagt
     3901 catgctaaag aaaaatgagc cttcaatagc agcattttca ttactgtctg aaccatgcac
     3961 tgcattggct tcaatacttt tagcataaag tttacggatt gtgccggcat cagcctttgc
     4021 agggtctgtt gcaccgatag tttttctgaa atcttcaacg gcattgtctt tttcaagaat
     4081 tgccgctaca ataggtccgg aagacatata ttttactaag tcattataaa atgtctttca
     4141 cggtgaacag catagaattc acctgcacgt tctgcagtta aacgtgtgta cttcattgct
     4201 ttaatcttaa atccggcttt ggtaatatga tctaaaatag cgccaatgta tccattgctt
     4261 accgcatcag gcttcagcat agtaaaagtg atgttactca taattaaatg tttgtatttt
     4321 tttgtggctg caaaagtagt tgatttcagt ttaggtttga ctgacgcaaa taacctacat
     4381 ttgtaaaaat cgcatttaaa aagtttatga taccaaaatt tgagcaacac agcatagagc
     4441 aattaaagaa tcttttatcg gaaccaaaaa gaattgtcat caccacacac tataaacccg
     4501 atggtgatgc tataggctca cttcttggat tgtataattt tttagttgct aaaaaacata
     4561 aagtcaaagc tgttgtgcca agtgattatc ctgcatcaat cagttttatg aagggcaatc
     4621 agaaagtgtt ggattatgaa gccaagccac gaaatgttgc taaagcattt tccgaagctg
     4681 aaattatttt ttgtcttgac tttaatgaat caaaaagagt ggaggcaatg gaagaagtaa
     4741 tgatgtcaaa caaagctttt aaagtagtta ttgaccatca tttatatcca aaaattgagt
     4801 gcgacatttt gttttcgttt cctaaatctt gtgccactag cgaattgatt tttcattttt
     4861 tatatgcatt agacaacaag tataaattga aaccagaggt ggcagagtgt atctataccg
     4921 gtatcatgac agatacaggt tctttcagat tttcttcgat gagtgctgat acgcatcgtg
     4981 ttttggcggc cttactcgat acaggcatga atcatgttaa aatacacgaa accatttcag
     5041 acaacttctc agaagaacga acacgttttc tcggttactg ccttaaagat aaaat
//
LOCUS       DAOOOI010000123         5039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3094_length_5039_cov_6.978732, whole genome shotgun sequence.
ACCESSION   DAOOOI010000123 DAOOOI010000000
VERSION     DAOOOI010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3094_length_5039_cov_6.978732"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PKX59_10370"
     CDS             <1..668
                     /locus_tag="PKX59_10370"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOZ91302.1"
                     /translation="VLDICFTVPQAEVAIRKMKPQFVFLDIMMEPFTGFDLLNRFDEI
                     DFEVIFTTTYQDFAYKAIKISAVDFLLKPFGEEEIKLAVKKLKERITSKQTLEYLNLL
                     KQNIQKKPEAMQVAIPVLTGYRFVEVSQILYVESSGKFNQVFLINGDDFVSTITLSEI
                     EKMVSDIMFFRSAKHIIVNLSYVNEYLKNQEKIILKNGKSVPLSRLRKSMFLAAVSRY
                     QNS"
     gene            complement(665..1930)
                     /locus_tag="PKX59_10375"
     CDS             complement(665..1930)
                     /locus_tag="PKX59_10375"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOZ91303.1"
                     /translation="MDIILKSVRIINPGTSHHQKTRDIVIVNGKIKSITTNANNDDGI
                     KELDLKGCFVMPGWFDMRANFREPGEEHKEDLISGTKAAAAGGFTEVLLMPSTLYPLQ
                     SKSDIEFVLNKCRNSLVNVHVAGCMTADRKGLDMAELYEMYVSGAIAFTDDKKVNDPG
                     MLVRALQYSNTFGARIISYADEPSLCNNYLINESPSSVRTGMKGCPAVAEEIGLNRII
                     RIAEYTDIAVHISGISTAGSVLQIRNAKSKGIKITAEVYAHNLMLDETLVETFDSKYK
                     VKPVLRSRADIQALKKGITDGVIDCVSSDHSPQDIEVKNCEYDYAAHGISSAETAFAT
                     LSTAFKGQLDAETLFHVLSGNPRKVLNINLPLFEDGEVANMTVYHPEKNWIFSEKEMV
                     SKSKNTPFNDTEFTGKVIGVINNNKINGF"
     gene            complement(1935..>2797)
                     /locus_tag="PKX59_10380"
     CDS             complement(1935..>2797)
                     /locus_tag="PKX59_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91304.1"
                     /translation="PDSNITPNGYENLLGKLNVENYSSLSSSSIGVAKLDFDNPLFQD
                     MFEKTDDRMSLPEVKQSYETVSSLRSGKNVLMQLQGGLSFLNYYHRKSGNVYQFTSAA
                     QPAAGTFVTHALFAPVLFKTLLLSVPSSQPTLFIGNNSDIKIPSSLQTNDVLHLINSD
                     TKTDIIPEFKTTNDGTLINLTGIEQAGFYQLKNLNDSTLSIIALNYNRKESNLKAFSD
                     DELRSVYGNGKFKSVEVVNPTGSGLTKFVKQYSSGISLWKYCILATLLFLIAETLLLR
                     SDRKMVTLKN"
     assembly_gap    2798..2807
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2808..3979)
                     /locus_tag="PKX59_10385"
     CDS             complement(<2808..3979)
                     /locus_tag="PKX59_10385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOZ91305.1"
                     /translation="MPIHFVYPFFLWFLAALSIPIIIHLFNFRRFKKIEFTNVRFLKE
                     IKEQTQSHSKLKHLLVLLMRLLALAFLILAFAQPFVPTKSTESSGQQTVSIYIDNSFS
                     MNLSGKEGSLLDEAKDKCRTIATSLPSSTKFQLLTNEFKGEQQRLIDRDELLELTDRI
                     KINPQSRNINEVLIRQTDALKKSNSDNRSSFIISDFQKNFFDIQPGVNDSLLKLNLLK
                     TNAEEVKNIAIDSCWLSAPVIQLNTPSELNVKVRNYGDEDAEVPVRFYINNSQKTVAS
                     VKVNAGTDATVKMNFTITQPGWQLAKAEIDDNPITFDDTYFLSFRIADKIPVYHIGSS
                     KNIFIDKLLANNSLADYKFSTQNAVDYAMLKKCNAVILTDLKDISSGMSDELARFV"
     gene            4076..4681
                     /locus_tag="PKX59_10390"
     CDS             4076..4681
                     /locus_tag="PKX59_10390"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOZ91306.1"
                     /translation="MLKSYFSDDVEAGIDEAGRGCLAGPVFAAAVILPRNFRNAELND
                     SKKLSALQRDNLRLVIEKEALAWAVAFCTHTEIDKINILQATYKAMHKAIAELKVFPE
                     RLLIDGNRFKKFKSVPHHCIVKGDGLFASIAAASVLAKTHRDEYMLNLHQKYPVYSWD
                     KNKAYATAVHVEAIKKYGLSSYHRKSFQLKSLQPQLIFPEN"
     gene            complement(4693..>5039)
                     /locus_tag="PKX59_10395"
     CDS             complement(4693..>5039)
                     /locus_tag="PKX59_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HOZ91307.1"
                     /translation="MHHDDFLLSHLCKGLIDRNLFKIKLQEKPFTQQETDTYLKKAVK
                     AFNITEEEASSLVFTGKIENSAYTTSQENIHILFKDNVIKDIADASDNLSISALSKPV
                     VKHYLCVDSGCL"
BASE COUNT         1526 a          875 c          883 g         1745 t
ORIGIN      
        1 ctgttttaga tatctgcttt accgtaccac aggctgaagt ggcaatcaga aaaatgaagc
       61 ctcagtttgt ttttctagac attatgatgg agccctttac cggatttgat ttattaaatc
      121 gttttgatga aatagatttt gaagtaattt tcacaactac ctatcaggat tttgcataca
      181 aagcaattaa aatttctgca gttgattttc ttctcaagcc atttggcgaa gaagaaatta
      241 aacttgctgt caaaaagtta aaagaaagaa ttacatctaa acaaactttg gagtacctga
      301 atttgctcaa gcaaaatatt cagaaaaaac cggaagctat gcaggtggct attcctgttc
      361 ttaccgggta cagatttgtt gaagttagtc agattttgta tgttgagagt agtggaaagt
      421 ttaatcaggt ttttttgata aacggagatg attttgtcag taccataacg ttgtcagaaa
      481 tagaaaaaat ggtgtctgat attatgtttt tccgatcggc aaaacacatt atcgtgaact
      541 tatcttatgt aaatgagtat ttaaaaaatc aggaaaaaat tattttgaag aatggcaaat
      601 cagttcctct ttcacgtttg agaaagagta tgtttttagc tgcggtttcc cgttatcaga
      661 attcctaaaa tccgttaatt ttattgttgt tgattacgcc tattactttt cccgtaaatt
      721 cagtgtcatt aaaaggcgtg ttctttgatt ttgaaaccat ttccttctct gaaaaaatcc
      781 agtttttttc cggatgataa acagtcatgt ttgcaacctc accgtcttca aatagcggca
      841 aattaatatt taaaacttta cgaggattac ctgataatac atggaaaagt gtttctgcat
      901 caagttgtcc tttaaatgct gtgcttaatg ttgcaaatgc tgtttcagca gaagagatgc
      961 catgtgctgc ataatcatat tcgcagtttt ttacttcaat atcctgagga gaatggtcac
     1021 tgcttacaca atcaattaca ccatctgtaa ttcctttttt taatgcctga atgtctgcac
     1081 ggcttcgtaa tacaggtttt actttgtatt tgctgtcaaa agtttctact aaagtttcgt
     1141 ctaacattaa gttatgtgca tatacttcgg cagtaatttt tattcctttt gactttgcat
     1201 tacgaatctg caacacactg cctgcagtgc ttataccact aatgtgtaca gctatgtcag
     1261 tatattctgc aattcgtatg attctattca gccctatttc ttcagccaca gccggacaac
     1321 ctttcattcc tgttcgtaca ctgctggggc tttcattaat cagatagttg ttgcataaag
     1381 aaggttcgtc agcatacgaa ataattcttg caccaaatgt gttactatac tgtaatgccc
     1441 taacaagcat tcccgggtcg ttaacttttt tatcatctgt aaaggcaata gctccggaaa
     1501 catacatctc ataaagttct gccatgtcaa gaccttttct atctgccgtc atacatcctg
     1561 caacatgtac attaaccaaa ctgtttcggc atttgttcaa cacaaattca atatcacttt
     1621 ttgattgtaa tggatataat gttgatggca ttaataaaac ttcagtaaat ccgcctgcag
     1681 cagcagcctt cgttccactg ataaggtctt ccttatgctc ttcaccgggc tccctgaaat
     1741 ttgctctcat atcaaaccat ccgggcatta caaagcaacc tttcagatca agttctttta
     1801 ttccatcgtc attattagca tttgttgtta tacttttaat tttcccgttg acaataacaa
     1861 tatcacgtgt cttttgatgg tgtgaagttc ccgggttaat aatccttacc gattttaaga
     1921 ttatatccat ggttttaatt tttaagtgtt accatttttc tgtcactgcg aagtaataat
     1981 gtttctgcta ttaaaaaaag caatgttgcc aatatacaat atttccagag tgaaatacct
     2041 gaagaatatt gtttaacaaa ttttgtgagg ccgctacctg taggattaac cacttctacc
     2101 gatttgaatt tgccattgcc ataaacactc cttaactcat catcactgaa agctttcaga
     2161 ttagattctt ttctgttgta atttaatgca attattgaaa gtgtgctgtc gtttaaattt
     2221 ttcagctgat aaaagcctgc ctgttcaata ccggttagat tgattagtgt tccatcattt
     2281 gtagttttaa attcaggaat aatatctgtc tttgtatcac tgttaattaa atgtaacaca
     2341 tcgtttgttt gcaatgacga tggtatttta atgtcactat tgtttccgat aaacagagta
     2401 ggctgtgaag atgggacact tagtaataag gttttaaaaa gtacaggtgc aaataatgca
     2461 tgtgtaacaa aagttcctgc agcaggttgt gctgctgaag taaactgata aacattgccg
     2521 gattttcgat ggtaataatt caaaaacgaa aggccgccct gcaattgcat taatacattt
     2581 tttcctgacc ttagtgatga tactgtttca taactctgtt ttacttcagg caaactcatt
     2641 ctgtcatctg tcttctcaaa catatcctga aacaatggat tgtcaaagtc aagtttggca
     2701 acaccaatac tgctactgct taaactactg taattttcta cattcagttt tcccaaaaga
     2761 ttttcatagc cattgggtgt aatatttgaa tctggaannn nnnnnnntca acaaaacgtg
     2821 caagttcatc actcattccg gatgagatgt ctttaaggtc agtaagaatt actgcattgc
     2881 attttttcag catagcataa tcaacagcgt tttgtgtgct gaatttgtaa tctgccaaac
     2941 tgttattagc taagagtttg tctatgaaaa tgtttttact gcttccaata tgataaacag
     3001 gaattttgtc tgcaatccga aaagatagaa aataggtgtc atcaaaagta atcgggttat
     3061 cgtcaatttc agcctttgcc aattgccatc ccggttgtgt aatggtgaag ttcattttaa
     3121 cggtagcatc cgttcctgca tttactttta ctgatgcaac tgttttttgt gagttgttaa
     3181 tataaaatct cacaggcact tcggcatctt cgtcaccata gtttctgact ttaacattca
     3241 actcagatgg cgtgttaagc tgaattaccg gagcacttag ccagcaggaa tcaatagcaa
     3301 tgtttttgac ttcttcagca tttgttttta ataaattcag ttttaagaga ctatcattga
     3361 cacccggttg aatatcaaag aagttttttt ggaagtcaga aataataaat gaggaacgat
     3421 tgtcagagtt tgattttttt aatgcgtcag tctgacgaat taatacttca ttgatgttac
     3481 gactttgtgg attaatttta atgcgatctg ttaattcaag tagttcatca cggtcaatga
     3541 gtcgttgttg ctcaccttta aattcatttg tcagtaactg aaactttgtt gatgatggga
     3601 gtgaagttgc aatggttctg catttatctt ttgcctcatc aagtaaggag ccttcttttc
     3661 cggaaagatt catgctaaaa gaattatcaa tataaatact gacagtttgc tgccctgatg
     3721 attctgttga ctttgtcgga acaaaaggtt gtgcaaaagc aagaatcaga aaagctaatg
     3781 ccaaaagtcg cattagcaat acaagcaggt gctttaattt agagtgcgat tgtgtttgtt
     3841 cttttatttc tttaagaaag cgcacattgg taaattcaat ttttttgaat ctcctgaaat
     3901 taaacagatg tataatgata ggaatagaaa gtgctgccag aaaccaaaga aaaaacggat
     3961 aaacgaaatg tattggcatt ttttattgag gaacgccaca aatatactta aatatgcacc
     4021 attttagtta aagttatgtg agtaattaaa ttaatgcaat tatttttgct cagagatgct
     4081 taaatcatat ttttctgatg atgtcgaagc cggtatagac gaagccggaa gaggttgtct
     4141 ggcaggccct gtttttgctg ctgctgtaat tctccctcgt aatttcagga acgctgagtt
     4201 aaatgactca aaaaaacttt ctgctttgca aagagataat ttacgtttag tcattgaaaa
     4261 agaggctttg gcatgggctg ttgcattttg cactcatacc gagattgata aaattaatat
     4321 tttacaggca acatataaag caatgcataa ggcaatagca gagttgaagg tgtttcctga
     4381 aaggttactt attgatggca acaggtttaa aaaatttaag tccgtacctc atcattgtat
     4441 tgttaaaggt gatggtctgt ttgcctccat tgctgctgca tcggtacttg caaaaacaca
     4501 tcgtgatgaa tatatgttaa atctgcatca gaagtaccct gtatatagtt gggataaaaa
     4561 taaagcctat gctacagcag ttcatgttga agcaataaaa aaatacggct tatcatcata
     4621 tcatcgaaaa tcgtttcaac ttaaatcatt acaaccccaa ttgatttttc ctgaaaattg
     4681 aaatagtttt ttctaaagac aaccggagtc aacacaaaga tagtgtttca caacaggttt
     4741 actaagtgct gaaatactca gattgtccga agcatcagca atatctttaa ttacattatc
     4801 cttgaaaagt atatgtatgt tttcttgact agttgtatat gcactgtttt cgattttccc
     4861 tgtaaaaaca agacttgaag cttcttcctc tgtaatgttg aaggctttta cggctttctt
     4921 taaataagta tctgtttctt gctgagtaaa tggtttctct tgtaatttta ttttaaaaag
     4981 atttctgtca attaaccctt tacatagatg agataaaaga aaatcatcat gatgcatcc
//
LOCUS       DAOOOI010000124         4999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3115_length_4999_cov_12.328883, whole genome shotgun sequence.
ACCESSION   DAOOOI010000124 DAOOOI010000000
VERSION     DAOOOI010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3115_length_4999_cov_12.328883"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..1033
                     /locus_tag="PKX59_10400"
     CDS             161..1033
                     /locus_tag="PKX59_10400"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOZ91308.1"
                     /translation="MTYFRIFSHPLKLQEIALLCSVSEKIAEQSLQVLIADEIVYCFD
                     KHFTTCKEVAQLAELRFVKEAEAEKYFAKLKKYARLLSTFPFVRGIAVSGSLSKGVMN
                     PDGDIDYFIITKPGRLWICRTSMVLFKKLFLFNSKKYFCVNYFVDSKNLQIRDENIFT
                     ATEFIHLLPVFEKDGVLADFVFQNEWVNKFYQRKPDWNKSYMVKGNSRVRSLLEFFLQ
                     LSVFNPIERVCHRLTILFWRKKFSTFKKEKFDLTMRSTQGISKHHPSDFQTKVLKEFQ
                     EEISKIHSVTLAEI"
     gene            1030..>1458
                     /locus_tag="PKX59_10405"
     CDS             1030..>1458
                     /locus_tag="PKX59_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOZ91309.1"
                     /translation="MKVLITHSYFLHFDKKQLELSKPYPPLATLLAASVLRNHSLEVH
                     FYDVSFDETVEKVKEQINVLKPDVLLIYDDGFNYLTKMCLTNMREAAFRMLHFAKSKG
                     IKTIISSSDSTDEYKKYLDAGADVVLLGEAEETLIELFNNR"
     assembly_gap    1459..1485
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1486..2490
                     /locus_tag="PKX59_10410"
     CDS             <1486..2490
                     /locus_tag="PKX59_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOZ91310.1"
                     /translation="NRLEVNEDIKGIAFTNNNIFYNTVRRNVNTQLDQLPFPAWDLLN
                     IKPYKKAWEKHGYFSINLSTTRGCPYKCNWCAKPIYGNRYNVRSTENVLNELKYMNRF
                     FKFDHVWFCDDIFGLRPGWLDSFCSRIKESGINVKYKIQCRADLLQREELIQKLAESG
                     AEEVWIGAESGSQKILDAMDKGITVEQIYHAVTLLKKYKIKPCLFLQFGYPGETKADI
                     QLTLKMLKELDPYDIGVSVSYPLPGTKFYENVKETMKEKTNWSDSDELLLMYSGEYNP
                     SFYKTLHRYVHKSFRAGKGQQELKKLIMLRRGSLQKALMTAYYKPASLFLKTKINLNQ
                     "
     gene            2487..3281
                     /locus_tag="PKX59_10415"
     CDS             2487..3281
                     /locus_tag="PKX59_10415"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ91311.1"
                     /translation="MIKQVMNTSTKAFSKQAAHFDEYNAINCITQYCRKEIYKIAEQH
                     LAPNSSILELNCGTGIDAIYFSKNHRVLATDGATGMIEALNKKISDNEITDIQSMQIS
                     FDQLDKLGKQKFDFIFSNFGGLNCTENLADVIKKLPALLKENGKIMLVIMPPICFWEH
                     LHVLSFNFKLAFRRWKKAGARASVEGVEFKTFYYSPERICRIVKKELKVVYLQGLCNV
                     MPPEYMYNHLKNHPFAFNLLAKADRFVNRFFPFNKTGDYFVIVLEK"
     gene            3288..4676
                     /locus_tag="PKX59_10420"
     CDS             3288..4676
                     /locus_tag="PKX59_10420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOZ91312.1"
                     /translation="MHSVSKQFYIIVILIAAGIFFITGYFAFSGLYGQDAYSYLLQSK
                     NLFTGDYHLNFYPPVYAFSGWVLQLVFQNYAVSLQLVSLIAVVVSTILVWKIVVLQYN
                     NERHALSFSIIIFLLSPFMLRASVLVMSDALAMMFCVACLFFYNIKSPSSTFWIYLFF
                     TLSILTRYATAPLLTPVLIFNLWHSIKRKQFVYLIGGVGMMLVLLGSNFLFWSTEAGG
                     AKHQWLHLWQIQNFFKSSFSTPDGIQDYFLPNVLFPLKALAYPGFMPLFFILLFFLRK
                     GDVLNNRMLVPLLSLLIYFIFLSGIPFQNDRFLVIAYPFVALIMYPAFSRILERFKPS
                     LLIVFMVCIVQITLAFRAVLPMYYRSIIENEIANALEPYQGKILYSFDMDIALKGKGL
                     NFEYRNLNDDKIANFEDGSLLLFNESKFQKQWKGMNPMLNFKFIQENCLLDHEKDFSD
                     DWKLYLIKPKQK"
     gene            4673..>4999
                     /locus_tag="PKX59_10425"
     CDS             4673..>4999
                     /locus_tag="PKX59_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91313.1"
                     /translation="MNVIWILPGFASNESDSTCIPPLQDLALSLQQQKVNLTILALDY
                     PFEQKSYCWNGIRVYAVGGANKKRFSRMLLLLRAWRLLISLINNHGVDIIHSFWLTWP
                     AILGQQF"
BASE COUNT         1599 a          685 c          957 g         1731 t
ORIGIN      
        1 tttgaagtta aagtgctgaa gcctgaaaat acttttgtca agcttatatc aggatttaaa
       61 aatccgttta atatttatat tgttaaatta aaaccgcaat ttattgaatc aaatttacaa
      121 ttgtaattcc gatgaagttc gattgtcaat attaaaggtg ttgacttatt ttcggatttt
      181 ctcacatcca ttaaagctac aagaaattgc attgctttgt agtgtttctg agaaaatagc
      241 tgaacagagt ttgcaagtat tgattgctga tgaaatagta tattgttttg ataaacattt
      301 tactacctgt aaagaagtgg cacaattagc cgaattgcgc tttgtgaaag aagctgaggc
      361 agaaaagtat tttgctaaac ttaaaaagta tgcaaggttg ttatcaactt ttccttttgt
      421 acgaggaatt gctgtttcgg gttcactgtc aaaaggagta atgaaccctg atggtgatat
      481 tgattatttt atcataacca aaccgggcag gctttggatt tgcaggacat caatggtgtt
      541 gtttaagaag ctgttcttgt ttaattctaa aaagtatttc tgtgtgaatt attttgttga
      601 ttcaaaaaac cttcagatta gagatgaaaa tatttttact gccacagaat ttattcattt
      661 gcttccggta tttgaaaaag atggtgtact tgctgatttt gtttttcaaa atgagtgggt
      721 aaataaattc tatcagcgaa agcctgactg gaataagagt tatatggtga agggaaattc
      781 aagagtaaga agtttattag aattttttct tcagttatct gtttttaatc cgattgaacg
      841 agtttgtcat cgcttaacta ttttattttg gcgtaaaaaa ttttcaactt tcaaaaaaga
      901 aaagtttgat ctgactatgc gaagcacaca aggtatttca aaacatcacc caagcgattt
      961 tcagactaag gtattaaaag aatttcaaga ggagattagt aaaattcatt cagttacgct
     1021 tgcagaaata tgaaagtgtt aatcactcat agttatttct tacatttcga caagaagcaa
     1081 ctcgaattat cgaaaccgta tccacctttg gctaccttgc tagcagcaag tgttttgcgt
     1141 aatcatagcc ttgaagtgca tttttatgat gtaagttttg atgaaactgt agaaaaagtt
     1201 aaagaacaaa tcaatgttct taaacctgat gttttactga tttatgatga tggatttaac
     1261 tatcttacaa agatgtgcct taccaatatg cgtgaagctg ctttcaggat gttgcacttt
     1321 gcaaagtcga agggcattaa gactattata tcaagcagtg attcaactga tgaatataaa
     1381 aagtatttgg atgcaggagc agatgtagtt ttgttaggag aggctgaaga gacattaatt
     1441 gagttattta ataacagann nnnnnnnnnn nnnnnnnnnn nnnnnaacag actggaggta
     1501 aatgaagata taaagggaat tgcgtttaca aataataata tcttctataa tactgtaaga
     1561 agaaatgtta atacgcagtt agatcaatta ccatttccag cttgggattt gttgaatatt
     1621 aagccctata aaaaggcatg ggaaaagcat ggctattttt caattaacct ttcaactacc
     1681 agaggatgtc catataaatg caattggtgt gcaaaaccta tttacggcaa caggtataat
     1741 gttcgttcaa ctgaaaatgt attaaatgaa ttaaaataca tgaaccggtt ttttaagttt
     1801 gatcatgtat ggttttgtga tgatattttt ggactgagac ccggatggtt agattctttt
     1861 tgtagtcgta tcaaagaatc tggaatcaat gtaaaataca aaattcaatg tcgtgcggat
     1921 ttgttacagc gtgaagaatt aatacaaaag ttagcagaaa gtggagccga agaagtttgg
     1981 ataggtgcag agtcgggctc acagaaaatt cttgatgcca tggataaagg gataactgtt
     2041 gagcaaattt atcatgctgt cacacttctt aagaagtata aaatcaagcc ctgtcttttt
     2101 cttcaatttg gttatcccgg tgaaacaaaa gctgatattc aactcacatt aaagatgctg
     2161 aaagaactag atccttatga cattggagtc tcagtcagtt atccattgcc cgggactaaa
     2221 ttttatgaga acgtaaaaga aacaatgaaa gaaaagacta actggagtga ttctgacgaa
     2281 ttgttattga tgtactcagg tgagtataac ccatcttttt ataaaacatt acatcgttat
     2341 gtgcataaat ctttccgtgc aggaaaagga caacaagaac ttaagaagtt gataatgctt
     2401 cgtagaggaa gtttgcaaaa agcattgatg actgcatatt ataaacctgc aagtttattt
     2461 cttaaaacaa aaattaactt gaaccagtga taaaacaagt tatgaataca tcaactaagg
     2521 cattctcaaa gcaagctgca cattttgatg agtataatgc gattaattgt attacacagt
     2581 attgtagaaa agaaatatat aaaattgcag agcaacattt agcgcccaat agtagtattc
     2641 tggaactgaa ttgtggaaca ggaattgatg caatttattt ttcgaaaaat catcgtgttt
     2701 tagctactga tggagccacg ggaatgatag aggcattgaa taaaaaaatt agtgataatg
     2761 aaattacaga tatacagtca atgcaaattt cttttgatca gttagataaa ttaggaaagc
     2821 aaaaattcga ttttattttt tctaatttcg gaggattgaa ttgtacggag aatttggctg
     2881 atgttataaa aaaactgcct gcattactca aggaaaatgg gaagattatg cttgtaataa
     2941 tgccaccaat ctgcttttgg gaacatttgc atgtattatc atttaatttt aaacttgctt
     3001 tcagacgatg gaagaaagct ggggcaagag caagcgttga aggagttgaa tttaaaacat
     3061 tttattatag tcctgaaagg atttgccgca ttgtgaaaaa ggagttaaag gtagtatatc
     3121 tacagggttt gtgtaatgta atgcctccgg aatatatgta caatcattta aagaaccatc
     3181 cttttgcttt taatttacta gctaaagcag atcggtttgt caatcgcttt tttccgttta
     3241 ataaaacagg agattatttt gtaattgtct tggaaaagta acagaaaatg cacagtgtaa
     3301 gcaaacaatt ttacattatt gtaatattaa ttgccgcagg catatttttt atcaccggat
     3361 attttgcctt cagtggtttg tatgggcagg atgcttatag ttatttgttg cagagcaaga
     3421 atttatttac cggtgattat catttaaatt tttatcctcc agtatatgca ttctccggat
     3481 gggtgttgca acttgttttt caaaactatg cagtttctct tcagttggtc agcctgattg
     3541 cagttgttgt atcaacaatt ttggtttgga agattgttgt actgcaatat aataatgagc
     3601 ggcatgcttt aagttttagt attattattt ttttacttag cccgtttatg ttgcgtgcat
     3661 ccgtgcttgt tatgagcgat gcattggcta tgatgttttg tgttgcctgc cttttctttt
     3721 ataatataaa gagtccttct tctacttttt ggatttattt atttttcaca ctttcaattt
     3781 taactcgtta tgctacagct ccattactta ctccggtttt aattttcaat ttgtggcatt
     3841 caattaaaag aaaacaattt gtttatttaa taggaggtgt cggtatgatg ctggttttac
     3901 ttggatcaaa ttttttattc tggtcaacag aagcaggagg ggctaagcat caatggttac
     3961 atttgtggca gattcaaaat tttttcaaga gcagtttctc aacgccagat ggtattcagg
     4021 attatttttt acccaatgtg cttttcccat taaaagcgct tgcatatcca gggtttatgc
     4081 ctttgttttt tatattgtta ttctttttac gaaaaggtga tgtattgaac aatagaatgt
     4141 tggtaccatt attatctctt ctgatttatt ttattttcct ttctggcatt ccgtttcaga
     4201 acgataggtt tctggtaatt gcatatcctt ttgtagcatt aatcatgtat ccggcattta
     4261 gcagaatatt ggagcggttt aaaccttccc ttttaattgt ttttatggta tgtattgttc
     4321 aaattacttt agcctttcga gcagttttgc caatgtatta tcgaagcatt attgaaaatg
     4381 agattgcaaa tgcattagaa ccatatcagg gaaaaatttt gtattctttt gatatggata
     4441 ttgcattgaa aggaaagggc ttgaatttcg aatataggaa tttgaatgat gacaaaattg
     4501 cgaattttga agatgggagt ttacttttat ttaatgaatc aaaatttcaa aaacaatgga
     4561 agggaatgaa tccaatgttg aatttcaaat tcatacaaga aaattgttta ttggatcatg
     4621 agaaagattt ttctgatgat tggaaattgt acctgattaa gcccaaacaa aagtgaatgt
     4681 aatctggata ttacccggct ttgcgtcaaa cgaatcggat tcaacctgta ttcctccatt
     4741 acaggatttg gctttatcat tgcaacaaca gaaagttaat ttaacgattc ttgctcttga
     4801 ttatccattt gagcagaaaa gttattgctg gaatggtatc agggtttatg ctgtaggagg
     4861 tgcaaataaa aagcgttttt caagaatgtt attgttgtta agagcatgga ggttgctaat
     4921 aagtcttata aataatcatg gtgttgatat aatacattca ttctggttaa catggcctgc
     4981 aattttgggt cagcagttt
//
LOCUS       DAOOOI010000125         4841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3233_length_4841_cov_8.474091, whole genome shotgun sequence.
ACCESSION   DAOOOI010000125 DAOOOI010000000
VERSION     DAOOOI010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3233_length_4841_cov_8.474091"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..467
                     /locus_tag="PKX59_10430"
     CDS             3..467
                     /locus_tag="PKX59_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HOZ91314.1"
                     /translation="MDIQKLDNAINVAKKEIVNRYDFHGSKTEIDLDKKEMQLKIVTE
                     DQMRMDAIRDIIISRSMKQGVDPKCFDFGKEQYASGNMVRKDVKIKKGIDKETAKTIV
                     KYIKETGMKVQASIMDEQVRVTGKKIDDLQQVIAEVKNKPVELPLQYVNFRS"
     gene            527..952
                     /locus_tag="PKX59_10435"
     CDS             527..952
                     /locus_tag="PKX59_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOZ91315.1"
                     /translation="MKKVTGIGGVFFKCKDPNKMREWYKQNLGLKTNPYGAVFEWFQG
                     ADNSKKGFTTWSPFDDKTKYFEPSSKEFMINYRVENIEALVAGLRNSGVNVVDEIETY
                     DYGKFVHILDPEGNKIELWEPNDVEYEKMGKEMNSETTK"
     assembly_gap    1120..1145
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1146..1856)
                     /locus_tag="PKX59_10440"
     CDS             complement(<1146..1856)
                     /locus_tag="PKX59_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOZ91316.1"
                     /translation="MYFCTNIKLLRKRKKRTQEVASLALGFSRSTLNSYENGAIANPT
                     VEALINFSNYFKISIDTLLRVDMSKLSDLQLRELELGYDSYVRGTKLRVLATTVDSRN
                     RENIELVPVKAKAGYTAGYNDPEFIRNLPTFQLPFLSHDRKYRTFQISGDSMLPIPDK
                     SFVTAEFVENWMEIKDGNAYIILTHDDGIVFKVVYNHISSNKKLLLKSLNPAYKPYEV
                     KINDVKEVWRFVNDISNEM"
     gene            1995..2582
                     /gene="purN"
                     /locus_tag="PKX59_10445"
     CDS             1995..2582
                     /gene="purN"
                     /locus_tag="PKX59_10445"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOZ91317.1"
                     /translation="MNKKRIAILASGSGTNAENIAAYFKNNEYAEVALILTNVATAGV
                     IVRAQKLNIPCLFMDKKTVSDGRLMLDLLKQHSIDLVVLAGYLKLMPAEVVSTFQHRM
                     INIHPALLPLFGGKGMYGHNVHEAVLQAGVAETGITIHYVNSRFDEGEIIFQKSMPVL
                     PDDTAERIASRIHRLEYEHYPSVIEHLAKGLFKDN"
     gene            2633..3037
                     /locus_tag="PKX59_10450"
     CDS             2633..3037
                     /locus_tag="PKX59_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HOZ91318.1"
                     /translation="MQFWLVKSEPFKYSWEQFVADGKTFWDGVRNNAARLHLLAMKKG
                     DQVLFYHSNEGLAVMGIAKVEKTAYTDPTADDTRRVGVELTPFKALKKPVTLAQVKDD
                     ARLKDIALVRIGRLSVMPLKKEEFNVILELSK"
     gene            3043..>4841
                     /locus_tag="PKX59_10455"
     CDS             3043..>4841
                     /locus_tag="PKX59_10455"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ91319.1"
                     /translation="MTIKRFFLAAFCMLAVSLVNAQTATIKGFVYAAENGEPVPFASV
                     FLKGTTFASFTDINGYYSMVKIPPGKYRVMATTLGYDTAFADVDLVADEMENRKLVMK
                     KKNVELKQVEVSAERQTKKTEVKIAVESVTPQEIKSLPSLGAEGDLVQYMQVVPGVVS
                     SGDQGGQLYIRGGTPVQNKVLLDGMVIYNPFHSIGLFSVFDTDILKNTDVYTAGFGAQ
                     YGGRISSVMDITTRDGNKKRIGGKVSAGTFLSKVLLEGPIKKAKDENDGTASFILSVK
                     NSYLDQTSKSIYKYASDDGLPYSFNDIYGKVVVNSASGSKVSFFGFNFQDKVNYSKVA
                     KYDWSSSGGGMNFVLVPGASKVLLRGNLAFSSYKIKLTEAEEKPRSSLVNGFNMGFNW
                     LYFLNNDEINYGIEVQGFKTDFEYFNYAKRQINQTENTTEIGIFFRYKKIINKLIIDP
                     SVRLQYYASISEFSPEPRLGIKYNLSDNIRLKAAGGLYAQNLIAANSDRDVVNFFYGF
                     LSAPDDLPSEFDGKSVKKKLQLARHAVVGVEYDISKHFDINIEGYIKDFNQLTNINRD
                     KVYDDTANNFDKPDSVKKSFIIEKGFTQGVDVV"
BASE COUNT         1504 a          854 c         1038 g         1419 t
ORIGIN      
        1 aaatagacat tcagaaatta gacaatgcca ttaatgtggc aaagaaggaa attgttaacc
       61 gctacgattt tcatggttct aagacagaaa ttgatttaga caagaaggaa atgcagttaa
      121 aaattgttac tgaagatcag atgcgtatgg atgctattcg cgacatcatc atcagccgaa
      181 gcatgaagca aggggttgac ccgaaatgtt tcgattttgg caaagagcag tatgcctccg
      241 gcaatatggt tcgtaaagat gtgaaaataa aaaaaggaat tgataaggaa actgccaaaa
      301 ccattgtgaa atatattaaa gaaacaggga tgaaggtgca ggcatctatc atggatgagc
      361 aggtgcgcgt tacaggcaaa aaaattgatg acttgcaaca ggttattgcc gaagtaaaaa
      421 acaaacctgt ggagttgcca ttacaatatg ttaatttcag gtcgtgagca agttgttgat
      481 tgtattagat tgaaaaattt tattctgaaa aacaaatcaa agaattatga aaaaggtaac
      541 aggtattgga ggtgtttttt ttaagtgtaa agaccctaat aaaatgcgtg aatggtataa
      601 gcaaaatctt ggattaaaaa ccaatcctta cggagcggtt ttcgagtggt ttcagggagc
      661 cgataactct aaaaaaggtt ttacaacatg gagcccgttt gatgataaaa caaaatattt
      721 tgaaccttca tcaaaggaat ttatgattaa ctacagagtt gaaaacattg aggcacttgt
      781 tgccgggtta cgtaactcag gagtaaatgt tgttgatgaa atagaaacct acgattacgg
      841 taaatttgtt cacatactgg atccggaagg aaataaaatt gaactatggg agcccaatga
      901 tgtggagtat gaaaagatgg gtaaggagat gaatagtgaa actacgaagt gatttttatt
      961 ggctaagccg ttctttcctc tctctcaaaa aactactctt tactaacatc ctgcacaccc
     1021 agcaggtctt tgattttatt catttcatat aggatgcgag caaccgtgtt agagagtttt
     1081 gcatattgct atcacgaatc agactattca tagctcaagn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnncattt cattgctgat gtcgtttaca aatcgccaca cttcttttac atcattaatt
     1201 tttacctcat aaggtttgta ggcaggattc aatgatttaa gcaataattt tttattgctg
     1261 ctgatgtgat tgtaaactac tttaaacact atgccatcat cgtgtgtgag tatgatgtag
     1321 gcattgccat cttttatctc catccagttt tctacaaact ctgccgtaac aaacgatttg
     1381 tccggtatgg gcagcatgct gtcgccacta atctgaaagg tgcggtattt tctgtcgtgc
     1441 gaaaggaagg gcagttgaaa tgtaggtagg ttgcgtataa actccgggtc gttgtagcct
     1501 gcagtatatc cggctttagc tttcacaggc accaactcaa tgttttcgcg gtttctgctg
     1561 tcaaccgttg ttgcaagaac cctaagcttt gtgccacgca cataggagtc gtagcctaac
     1621 tccagttcgc gcagttgcaa gtcgctgagt ttactcatgt caacacgcag caaggtgtct
     1681 atagaaattt taaaataatt agagaaattt atcagtgcct caactgtagg gttggcaata
     1741 gcgccattct catagctgtt taatgtagag cgcgaaaatc ctaaggcaag tgatgctact
     1801 tcctgtgtac gctttttgcg tttgcgaagt agttttatgt tggtgcagaa atacatgtta
     1861 tgtggtttaa tgaatttact acaaatttaa tatacaggct atataataac caaatgaatg
     1921 attgtaataa tgccaaaaaa atatctaagc ctatcattta cagaatgcta tttttacacg
     1981 ctcataattg tgcaatgaac aaaaagcgaa tagccatatt agcatcgggg tcgggcacca
     2041 atgccgaaaa tattgctgct tattttaaga acaatgaata tgcagaagtg gcattgattc
     2101 tcaccaatgt ggcaacggca ggtgtgatag tacgcgcaca gaagttaaac attccttgtc
     2161 tttttatgga caaaaaaact gttagcgatg gccgtctgat gcttgatttg ttaaagcaac
     2221 atagcattga tttggttgtg cttgccggat acctaaagct gatgcctgcc gaagttgttt
     2281 caacttttca gcatcgcatg ataaatattc atcctgcatt gctgccactt ttcggaggca
     2341 aaggcatgta tggtcacaac gttcatgagg cagtgttgca ggcaggtgtt gcagaaacgg
     2401 gcataaccat tcactacgta aacagccgct ttgacgaggg tgaaattatt tttcagaaaa
     2461 gtatgcctgt gctacctgac gacacggcag agcgtatagc gtcacgtatt catcgtcttg
     2521 aatatgagca ttacccatcc gtaattgagc atttggcaaa gggtttattt aaggataatt
     2581 aacaaatccg tattcgatta tttttatttc tttgtccgac tttaaaaata gtatgcagtt
     2641 ttggctggta aaatcagagc cgtttaaata ttcgtgggag cagtttgttg ccgatggaaa
     2701 aacattttgg gacggtgtgc gcaacaacgc tgcacggctg catttacttg ccatgaaaaa
     2761 aggcgatcag gttttgtttt atcacagcaa tgaaggcctt gccgttatgg gtattgctaa
     2821 agttgaaaag acagcatata ccgatcccac tgctgacgat acacgtcggg taggtgtgga
     2881 gttgacacca ttcaaagcat tgaagaagcc tgtaacattg gcacaggtaa aagatgatgc
     2941 acggttgaaa gatattgcat tggtgcgcat aggccgcctg tcggtaatgc cacttaaaaa
     3001 agaagagttt aatgtaattt tagaattaag taaatagttt ttatgacgat aaaaagattt
     3061 tttttagctg ctttctgtat gcttgccgtt tctctggtaa atgcacaaac agccaccatc
     3121 aaagggtttg tttatgctgc agaaaatggt gagcctgttc cctttgcaag tgtttttctg
     3181 aagggaacaa cctttgcctc ttttacagac attaacggtt attattccat ggtgaaaatt
     3241 cctccaggaa aataccgcgt aatggcaaca acattaggct acgacactgc ttttgcagat
     3301 gttgatcttg ttgccgatga aatggaaaac cgtaaattag tgatgaagaa aaaaaatgtg
     3361 gagttgaaac aggttgaggt atcggcagag agacaaacaa aaaaaacaga agttaaaatt
     3421 gcagtagagt ctgtaacacc tcaggaaatt aaatctctgc caagtcttgg tgccgaaggt
     3481 gatttggtac agtacatgca ggtagtgccc ggtgttgtaa gcagtggcga tcagggaggg
     3541 caattgtata tccgcggagg aactcctgta caaaacaaag tgttgctcga tggtatggtc
     3601 atctacaatc cgtttcatag cattggcttg tttagtgtgt ttgatacaga cattttaaaa
     3661 aataccgatg tttatactgc aggttttggt gcgcaatatg gtggacgcat ttcatcagtg
     3721 atggacatca ccacacgcga tggaaataaa aaacgtattg gcggaaaagt ttctgcaggc
     3781 acattccttt ctaaagtttt gttagaaggt ccgataaaga aagctaaaga cgaaaatgac
     3841 ggaacagcgt ctttcattct ttcagtaaaa aattcctacc tcgaccagac ttcaaaaagt
     3901 atttacaaat atgcttccga tgacggcttg ccgtattctt tcaatgatat ttatggaaaa
     3961 gtggttgtca acagtgcaag cggaagtaag gttagctttt tcggatttaa ttttcaagat
     4021 aaagtaaatt acagcaaggt agcaaaatac gactggagtt cttccggtgg tggaatgaac
     4081 tttgtgttgg tgcccggtgc atcaaaagta ttgctgcgtg gtaacctcgc tttctccagc
     4141 tataaaatta agcttacaga agccgaagaa aaaccacgct ccagcttagt gaatggattt
     4201 aacatgggct ttaactggct ttacttttta aataatgacg aaattaatta tggtattgaa
     4261 gtgcaaggct tcaaaaccga tttcgaatat tttaattatg ccaagagaca aatcaatcag
     4321 acagaaaaca ctactgaaat cggaatcttc tttcgctaca aaaaaatcat caataaactc
     4381 atcattgatc cttccgttcg cttgcagtat tatgcatcca tctctgaatt ttctcctgaa
     4441 cctcgccttg gaattaaata caacctgagc gacaacatca gacttaaagc tgccggtgga
     4501 ctgtatgctc aaaatcttat tgctgccaac agcgaccgcg atgtggtaaa ctttttctat
     4561 ggtttccttt ctgcacctga tgatttacct tctgagtttg atggaaaatc cgttaagaaa
     4621 aaactgcagc ttgcacgtca tgcagttgtt ggtgttgaat atgatatcag caaacatttt
     4681 gatattaaca tcgaaggcta cattaaagat tttaatcagc ttaccaatat caaccgcgac
     4741 aaggtttatg atgacactgc aaacaacttc gacaagcccg actccgttaa gaaaagtttt
     4801 atcatcgaaa aaggatttac acaaggtgtt gacgttgttt t
//
LOCUS       DAOOOI010000126         4840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3234_length_4840_cov_48.491745, whole genome shotgun sequence.
ACCESSION   DAOOOI010000126 DAOOOI010000000
VERSION     DAOOOI010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3234_length_4840_cov_48.491745"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="PKX59_10460"
     CDS             complement(<1..1475)
                     /locus_tag="PKX59_10460"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOZ91320.1"
                     /translation="MNKTSFHELSLSHEMLQAIDEMGFTEASPIQAQAIPVLMQGQDV
                     IGQAQTGTGKTAAFGIPLLEMLDPKVKATSALVMCPTRELAVQVAQEIKKLAKYKKGI
                     AVLAVYGGEQIQKQINALKRPVHVIVGTPGRIMDHLDRRTINLSKIQMVVLDEADEML
                     NMGFREDMEAILQHAPDERQTVLFSATMPKPILEITKRFQTNPKLVKVTKQELTVSSI
                     EQIYYDIPSSQKADVITQLVELHNLKLMLVFCNTKRKTDEVTEQFQQLGYKAEAIHGD
                     LRQNQRNNVLARFKKGDVNMLVATDVAARGIDVENVDAVFNFDLPLDEENYVHRIGRT
                     GRAGRSGKAFSFVSGRNDMAKIREIEDYSKVRIERHQLPSGKERIELAKIKLAARLKV
                     LIEEGDMEQYDNMFATFKQEGLTLHQLAAALLKMHFAPMLQQSQNKSSSKSSGREERS
                     GREERSGRGNRDSGGGKGKRGKHSGEADMIRLFLSVGKKDKV"
     gene            complement(1542..2252)
                     /locus_tag="PKX59_10465"
     CDS             complement(1542..2252)
                     /locus_tag="PKX59_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOZ91321.1"
                     /translation="MDLQLTPEQVFCNELLIQDNDCLVFIFCSDSFIAVESELKKLLS
                     NDETERLLKFKFEKDRQTYCIAHGLKRVLLAALLNTKEQVEICSHSNGKPFINHQKHN
                     MPLHFSLSHSEKMIALAFQKNSPLGVDIEKIQPADKYGNVIQHYFTSDEQKLISRSDN
                     DTFFKIWTQKEAIGKATGQGIANLPEIVIPDDYELSTASLGGYILSTCFFRGNEISFK
                     TIHAQDVLNQINILSLRP"
     gene            complement(2261..>4840)
                     /locus_tag="PKX59_10470"
     CDS             complement(2261..>4840)
                     /locus_tag="PKX59_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HOZ91322.1"
                     /translation="FIEWPVVKENKLSNECIHTLIEKQASANPDTNAIWFNGQSITYG
                     QLNQRANQLAHYLIQSGVKQETLVGICHDRTDDLIVAMLAVLKAGGAYVPLDPNYPAD
                     RIAYILSDAKAPLLISSSKYIDQLGETGTKLVCLDKEVTEISKEKITNPDVEITPDQL
                     AYIIYTSGSTGKPKGVAIEHRNAIALIDWAQTVYSSEELAGVLASTSICFDLSIFEIF
                     FTLSNGGRIVLVKDAMALNALPENAGVTLVNTVPSAIAELLKLKDGVPSSVKTINLAG
                     EPLPVELVQKIYSQTTVKKVYDLYGPSEDTTYSTFTLRKPDGPYTIGKAITNSQVYIL
                     DKNRRPVPVGIAGELYMAGDGLARGYLYKPEMTEERFVANPFVLGQRMYKTGDLGKFM
                     SDGNIEFLGRIDNQVKIRGFRIELGEIESMLRLRKEIKELVVMAREDQPGNKILVAYL
                     STNAGKQIDVNELRQYLRTELPEYMIPSVFMILENIPLTPNGKIDRKALPVPEVEVKT
                     ESANFEEPHNPLESLLAGIWSDVLGVDKIGIHDNFFELGGHSLLGIRMMGAIEQKTGV
                     KLDYPTLFRASTISQLAKVMNAEELNIDWPIVVPLQPNGKARPLFFVHMHNGNIQRWR
                     VLLKHLDKDQPVYAIQPRGLDEKYDYHYSVEAMASFYIDEIKKIQPVGPYRIAGLCFG
                     GTTAFEMARQLKTKGETTELVFMINNYAPPSNPMQYKIRETWDEFKGMTLEDKLEFAL
                     EKTKNAGKKVLSKLTKVFDSGSTTASEVKVNTKPDIRWVNSVALLNYQPEGEYSEKVI
                     LIKTGEEVAKHYDETLGWKRLISGEIEMHQITGSDNDTIITHKEYYTQLAQYLNQAIN
                     QIK"
BASE COUNT         1380 a         1000 c          815 g         1645 t
ORIGIN      
        1 actttatctt ttttgccaac gcttaaaaat aaacgaatca tatcggcttc gccactgtgt
       61 ttaccgcgtt tgcctttgcc accaccgcta tcacgattac ctctgccgga tctttcctct
      121 ctgccggatc gttcttctct acccgatgat ttactgcttg acttattttg actttgttgc
      181 aacataggtg caaaatgcat tttaagcaat gccgctgcaa gttgatgtaa tgttaatcct
      241 tcctgcttga aagttgcaaa catgttgtca tattgctcca tatcgccttc ctcaataaga
      301 actttcaatc ttgctgccaa tttaatttta gccaactcta tacgctcctt tcctgatggc
      361 agctgatgtc tttctatcct cacttttgaa taatcttcaa tctcacggat tttagccata
      421 tcatttcgcc cggaaacaaa actgaaagct ttacccgaac gaccggcacg gcctgttctg
      481 ccgatacgat gcacataatt ttcttcatcg agtggaagat cgaaattaaa tacagcatca
      541 acattctcaa catcaatacc tcgtgcagct acatctgttg ccacaagcat attcacatca
      601 cctttcttga atcgtgccaa aacattattt ctttgatttt gtcgcaagtc accatgaata
      661 gcctctgcct tataacccaa ttgctgaaac tgctcggtta cctcatcagt tttacgtttg
      721 gtattacaga atactagcat tagttttaaa ttgtgtaact ctactaattg tgtaattaca
      781 tcagctttct gtgacgatgg aatatcgtaa taaatctgtt caattgatga tacagtaagc
      841 tcctgcttgg tgaccttaac taacttagga ttggtttgaa aacgtttggt gatttcaaga
      901 ataggtttag gcatagttgc agaaaacaac accgtctgtc tttcgtcagg ggcatgttgc
      961 aaaattgctt ccatatcttc tctgaaaccc atattcaaca tttcatcagc ctcatcaaga
     1021 actaccatct gaattttaga caggtttata gttcttctgt ccaaatgatc cataattctg
     1081 cccggtgtac caacaattac atgtacaggg cgttttaatg catttatctg cttttgaatt
     1141 tgctcgccac cataaacggc aagcacagca ataccctttt tatacttggc taatttttta
     1201 atttcttgtg cgacctgtac ggctaactcg cgagttgggc acattaccaa tgctgaagtt
     1261 gccttaactt tagggtcgag catttcgagt aaaggaatac caaaagctgc tgtttttccg
     1321 gtacctgttt gcgcctgccc tattacgtcc tgaccctgca tcaatacagg tatggcttgt
     1381 gcttgtatgg gcgaagcctc agtgaaaccc atttcgtcaa tagcctggag catttcatga
     1441 cttaacgaaa gctcatggaa agaagttttg ttcatttgaa gatggaagaa ccggaattat
     1501 cagtgttttt caaaaaatta tccggttgat tataatgtta attatgggcg caaagatagt
     1561 atattaatct gatttaaaac atcctgtgca tgaattgtct taaaactaat ctcattacct
     1621 ctgaaaaaac atgtacttaa aatatatccg cctaaagatg cagtacttaa ctcatagtca
     1681 tccggaatga caatttcagg taaattggct ataccctgac ctgtggcttt tccaatggct
     1741 tccttctgag tccagatttt aaaaaaggta tcattgtctg accgggaaat tagcttttgc
     1801 tcatccgaag taaaataatg ctgaatcaca tttccatatt tatctgccgg ttgaattttt
     1861 tctatatcta cacccaacgg gctatttttc tgaaatgcca gtgctatcat tttttctgaa
     1921 tgactcaaac taaaatgtag cggcatatta tgtttttgat gattaataaa tggtttccca
     1981 ttgctatgtg aacaaatctc tacttgttct tttgtattga gcaatgcagc aagtaaaact
     2041 cttttcaatc catgtgcaat gcaataggtc tgtctgtctt tttcaaactt aaatttaaga
     2101 agtctttctg tttcatcatt gcttaaaagt tttttcaatt cactttctac tgctataaat
     2161 gagtctgaac agaaaataaa aactaagcaa tcattgtcct gaattaataa ttcattgcaa
     2221 aaaacctgtt ccggagttaa ctgcaaatcc atctcagcgg ttatttaatt tggtttattg
     2281 cctgatttaa atactgcgct aactgcgtat aatattcttt atgggtaatg attgtatcgt
     2341 tatcgcttcc ggtaatctga tgcatttcta tctcgccact tataagtcgt ttccatccca
     2401 atgtctcgtc ataatgtttg gcaacttctt caccggtttt gattaaaatt actttttcag
     2461 aatattcacc ttcgggctga taattgagca aggctactga atttacccaa cgtatgtcag
     2521 gcttagtatt tactttaact tcagaggctg tagtgctgcc ggaatcaaaa accttagtca
     2581 gtttgcttaa aactttttta cctgcatttt tagttttttc aagtgcaaat tcaagtttat
     2641 cttccaaagt catgccttta aattcatccc atgtttcgcg gattttatat tgcattggat
     2701 tggatggtgg tgcataattg ttaatcataa aaacaagttc ggtagtttca ccttttgttt
     2761 ttaactgacg agccatttca aatgcagttg ttccaccaaa acataatccc gcaattcggt
     2821 atggacctac aggttgaatc tttttaatct catcaatata gaaagatgcc attgcctcaa
     2881 cagaataatg atagtcatat ttttcatcaa gtccgcgggg ttgaatagca taaacaggtt
     2941 ggtctttatc taaatgtttt aatagcactc tccatcgctg aatgtttcca ttgtgcatgt
     3001 gaacaaagaa caacggtctt gcttttccat taggttgcaa tggaaccact ataggccagt
     3061 caatgttcaa ctcttctgca ttcatcacct tagctaattg cgagatggtt gaagcacgga
     3121 ataatgttgg ataatccaat ttaactcctg tcttctgctc tatagctccc atcattctaa
     3181 ttccaagaag tgagtgtcct cccaactcga aaaaattatc gtgaatacca attttatcaa
     3241 ccccaagcac atcactccaa atacctgcaa gtaaactttc taaagggttg tgaggctctt
     3301 caaaatttgc actttcagtt ttaacttcca cttcgggaac aggtaatgct tttctgtcaa
     3361 ttttaccatt tggagttaaa ggaatatttt caagaatcat aaataccgat ggtatcatgt
     3421 attcgggaag ttctgttctc agatattgcc gtagttcatt gacatcaatc tgctttcctg
     3481 cattagtaga aaggtatgca actaaaattt tatttcctgg ttgatcttca cgtgccatta
     3541 ctacaagttc ctttatctct tttcgcaatc gcaacattga ttctatttca ccaagttcaa
     3601 ttctaaatcc gcgaattttt acctgattat ctattcggcc taaaaattca atatttccat
     3661 cagacataaa cttcccaagg tcaccggttt tgtacatcct ttgtccgaga acaaacggat
     3721 tagccacaaa acgctcttca gtcatttcag gtttatagag ataaccgcga gcaagaccat
     3781 caccagccat ataaagttct cctgcaatac caacaggaac aggtcgtctg tttttatcta
     3841 atatataaac ctgcgaattg gtgatagctt ttcctattgt atatgggcca tcgggttttc
     3901 tgagtgtaaa tgtactgtag gtagtatctt cagaagggcc atataaatca taaactttct
     3961 tgacagtagt ttgcgaataa attttctgaa ccaattcaac aggcaaaggc tcacctgcaa
     4021 gatttattgt tttaacgctt gaaggaactc cgtctttaag tttgagtagt tctgcaattg
     4081 cagaaggcac tgtgttaacc aatgtaacac ctgcattttc aggtaaggca tttaaagcca
     4141 tggcatcttt aactaaaaca atgcgtccac cgttgcttaa tgtaaaaaat atttcgaaaa
     4201 tggataagtc aaaacagata gatgtagaag caagcacacc tgcaagttcc tctgaagaat
     4261 aaactgtctg tgcccaatca attaaggcaa ttgcattgcg gtgttcgata gctacacctt
     4321 taggttttcc tgttgatccg gatgtgtaga taatatatgc taactgatca ggagtaattt
     4381 caacatcagg atttgttatt ttctctttag aaatttcagt tacttcttta tctaaacaaa
     4441 ctaactttgt tcctgtttca cctaactgat ctatgtattt tgacgatgaa atcaagagag
     4501 gtgcttttgc atcgctcaag atataggcaa ttctatctgc aggataattt ggatctaaag
     4561 gcacgtaggc accacctgct tttaatactg caagcatggc aacaattaaa tcgtctgtcc
     4621 tgtcgtgaca aattccaact aatgtttcct gtttaacacc agactgtatt aaataatgtg
     4681 ctaactgatt ggctcgttga tttagttgtc cgtaagttat tgattgccca ttaaaccaaa
     4741 ttgcatttgt atcaggattt gccgatgctt gcttttcaat taaagtatga atgcactcat
     4801 tgctaagttt attctctttt acaactggcc attcgataaa
//
LOCUS       DAOOOI010000127         4831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3241_length_4831_cov_6.889028, whole genome shotgun sequence.
ACCESSION   DAOOOI010000127 DAOOOI010000000
VERSION     DAOOOI010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3241_length_4831_cov_6.889028"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..991)
                     /locus_tag="PKX59_10475"
     CDS             complement(143..991)
                     /locus_tag="PKX59_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HOZ91323.1"
                     /translation="MQKFTSSLMALIIATTTTSMAQLKTPQPSPTQSLTQAFGLGEIK
                     IEYSRPSVKGRTIFGDLVPYGKIWRTGANQSTKVTFGADTKVNGNDIAAGTYALYTIP
                     NTTDWEIIFYKDLTLGGNVGDYKQTDEMMRFKVKPVATADKMETFTININNIAPTSCS
                     IDLLWDKTKVSIPVTTNIDENVMKQIDKAMGDNKPYYQAANYYYENDKDLNKAREWIN
                     KAVENNPKAYWVLHTKAKIEYKLKNYTEATKAAEQSLALAKESSDNSYIKMNEKLIAD
                     VKKMGK"
     gene            complement(1097..1948)
                     /locus_tag="PKX59_10480"
     CDS             complement(1097..1948)
                     /locus_tag="PKX59_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOZ91324.1"
                     /translation="MKWMGRRESDNVEDRRRFSGGKIALGGGLVGIIVIVINLLMGGD
                     PNQLLQQMQQQATTQTTTTESSAADDEMASFIKVALADNEDIWKKIFQENELEYREPK
                     LVLFRDATQSGCGNASSAVGPFYCPADEKVYIDLSFFDELKSRFGAEGGDFAVAYVLA
                     HEVGHHVQHLLGTSDKVHQMQEGLSETEANKLSVALELQADFYAGVWANQNQKLNNVL
                     EDGDIEEALSAASAVGDDKIQKKAQGYVVPDAFTHGTSEQRMYWFKKGYQTGKLQEGN
                     TFKELNI"
     gene            2033..3790
                     /locus_tag="PKX59_10485"
     CDS             2033..3790
                     /locus_tag="PKX59_10485"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOZ91325.1"
                     /translation="MLHNNLNMQNALKFILAYLFFTGNVSAQEILPNYMTQKEMLQMP
                     SYLQQLSNQLKNNQIAAPPSSPVRTIAEWEELQGILIGWTSYPSILREIVRAAKTECR
                     VYVVTNNEQSVINYLNSGNVDTINVSFVNTPFNSVWSRDYGPWSAYTNDVDTLVTIDW
                     IYNRPRPDDDNVPVAIAGLLNTPLYQTTMSPYSLVHTGGNFMCDGFGTGFSSNLIVNE
                     NPSLTISQIDTIMKQFMGINRYIKMPTLPYDAIHHIDMHMKLLNEETILMGQYPQGVA
                     DGPQIEANLLYVINNFNSVFGTPYKIIRIPMPADNGAYPNTNGDYFTYTNSSFINNTI
                     IVPTYNIPQDSTALRIYRDALPGYNVIGINSLQSIGAIGALHCITKDLGTNDPLLISH
                     QQLSNTYDTINPYHVEAFIKHRSGIQSATLYYRTDTLQPYNTVSMNAISGMSDYFETY
                     IPAQSGGTRIYYYIEAYSNTSKHQTRPMPAPQGYFYFDVLQTTGITEVLNQLSVGQIF
                     PNPASAVTCLPYYSRIGTLLQIKISDVTGKMVYESQNTTKSNGQNFAFFDVSQFKPGV
                     YFVKSETNSSINIQKLIVK"
     gene            complement(3829..4695)
                     /locus_tag="PKX59_10490"
     CDS             complement(3829..4695)
                     /locus_tag="PKX59_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91326.1"
                     /translation="MTKIYLKVFLFFLASLCSLGSLNAQTQTVVVGTSSLISAFSPIV
                     KSYECVAYGAIYLSTEIGYSGSISSFGFNRVDGTDTLPFDSVSIFMKQTAFTSLTAGN
                     VDTTGYTLVYEGPFPNNGGIGWREVALDTSFTYSTSSNLSVMVFKHTQTATLSTPVSP
                     RWYYTSVSSNRSRRYYGAIPISSVTSLSATNVLSDIRITVGTVGIVDISAGLFSVYPN
                     PTNGIIHIVLHNSEISSLKISDINGSIVKEMYNLKDGSLDLTNLKDGVYNLQFFDKSK
                     NNIATRIIVVKR"
BASE COUNT         1513 a          915 c          790 g         1613 t
ORIGIN      
        1 atttcattgg aatttggctt tttacttagg ataaataaaa aagggaaaca actattatgt
       61 tgttcccctc taacctaaaa acaataaacc ctccacaaat gtaggagaat accgttttga
      121 ggatcagatt gttaataact ttttatttac ccattttctt aacatctgca atcaactttt
      181 cattcatttt aatgtaactg ttatcgctgc tttcctttgc taaagccaga ctttgctctg
      241 ctgcttttgt tgcttcggta tagtttttca gtttatattc aattttagct ttagtgtgta
      301 aaacccaata tgcctttggg ttgttttcca cagctttatt tatccattcg cgtgctttat
      361 tcaggtcttt atcattttcg taataatagt ttgcagcctg atagtagggc ttgttatctc
      421 ccatagcctt atctatttgc ttcataacat tctcgtcaat attggtagta acaggaatac
      481 tcacttttgt tttatcccat aacaaatcaa tactgcatga agttggtgca atgttattga
      541 tattgatagt gaaagtttcc atcttatcgg cagtagcaac cggcttcacc ttaaatcgca
      601 tcatttcatc tgtttgttta tagtcgccta catttccgcc aagagttaaa tctttgtaaa
      661 aaataatttc ccaatctgtt gtgtttggaa ttgtgtagag tgcataagtg ccggccgcaa
      721 tatcattacc atttaccttg gtatctgctc caaaagttac tttggtagat tgattggctc
      781 ctgtgcgcca tattttacca taaggaacaa ggtcgccaaa aattgttctt ccttttactg
      841 atggtctgct gtattcgatt ttgatttcgc caagaccaaa tgcctgtgtc agactttgag
      901 ttggacttgg ttgtggcgtt tttaattgcg ccattgaagt tgttgttgtg gcaatgatga
      961 gtgccattaa cgatgatgtg aatttttgca tgataattta aatttgattt tgaattttat
     1021 acctaaacat aaaaacccga cagacaacat ttagttttta tcgggtttaa aaagtttgca
     1081 tttgagattg aataaattaa atgttcagtt ctttaaaagt attaccttct tgtaattttc
     1141 ctgtctgata gcctttttta aaccagtaca ttctctgttc agaagtgcca tgagtaaaag
     1201 catcaggcac aacatagcct tgtgcttttt tttgaatctt atcatcgcca actgcacttg
     1261 ctgcacttaa tgcttcttca atatcaccat cttccaacac attgtttaat ttttgatttt
     1321 gattagccca aactcctgca taaaaatctg cctgaagttc taaagcaact gacaatttat
     1381 ttgcttctgt ttccgataaa ccttcctgca tctgatgtac cttatccgat gtgcctaaaa
     1441 gatgttgaac atgatgacct acttcatgtg ccaatacata agcaacagca aaatcaccgc
     1501 cttctgctcc aaaacggctt ttcaactcat caaaaaatga aaggtcaata tatactttct
     1561 catcggccgg acagtagaac ggcccaacag cagagcttgc attgccacaa ccggactgtg
     1621 ttgcatctct aaaaagaacc aatttaggct cacgatattc taattcgttt tcctgaaaaa
     1681 ttttcttcca gatatcttcg ttatctgcta atgctacttt tataaaactt gccatttcat
     1741 catcggcagc agatgattcc gtggtggttg tctgagttgt tgcctgctgc tgcatttgtt
     1801 gtagaagttg attaggatca ccacccatca gcaagttgat gacaatgaca attatgccaa
     1861 ctaatccacc acctaaggca attttacctc ctgaaaatct cctgcggtct tcaacattgt
     1921 cgctttcgcg cctgcccatc catttcataa tatctgtttt acagagcaaa taaagcaatt
     1981 attttggaaa tactatttaa tgacggcata aatctgaaag tgattatatt tgatgcttca
     2041 taataacctc aacatgcaaa atgcacttaa atttatttta gcatatctct ttttcacagg
     2101 aaatgtttct gctcaggaaa ttcttccaaa ctatatgact caaaaagaaa tgttgcagat
     2161 gccatcatat ctccaacaat tgtctaatca attaaaaaat aatcagattg cagctccacc
     2221 atcatcaccc gttcgaacta ttgctgagtg ggaagagctg caaggaattt taattggctg
     2281 gacaagttac ccttctattt tgcgtgaaat tgtacgtgct gctaaaacag aatgccgggt
     2341 ttatgttgta accaataatg agcaaagtgt aattaactat ttaaacagtg gaaatgtaga
     2401 tacgataaat gtaagttttg tgaatacccc tttcaattca gtatggtcac gcgattacgg
     2461 tccatggagt gcttacacaa acgatgttga cactttagtt actattgact ggatatataa
     2521 tcgtcctcgt cctgacgatg ataatgttcc ggtggcaatt gccggattac taaatacgcc
     2581 attataccaa accacaatgt cgccatacag tttggttcat acaggtggta attttatgtg
     2641 cgatggattt ggaacgggat tctcaagcaa tcttatagtg aatgaaaatc cctcgttaac
     2701 cataagtcag atagatacta tcatgaaaca atttatgggc attaacagat atattaaaat
     2761 gcctacatta ccttatgatg ctatccatca tattgatatg cacatgaagc ttcttaatga
     2821 agaaacaatc ttgatgggac aatatcctca aggtgttgca gatggtcctc agatagaagc
     2881 caatctgctt tatgttatca ataattttaa ttccgtattt ggcacacctt ataaaataat
     2941 ccgcattcca atgcctgctg acaatggtgc ttatccaaat acaaacggtg attattttac
     3001 ttacaccaat tcatcgttca tcaataatac tattattgtt cctacttata atattcctca
     3061 agacagcaca gcattacgga tatatagaga tgcattaccg ggatataatg ttattggtat
     3121 taactcactt caaagtattg gtgctatagg agcattgcat tgtatcacta aagatttagg
     3181 cactaatgat ccgcttctga tttcacatca acaacttagc aacacttacg atacaattaa
     3241 cccctatcat gttgaagcat ttattaaaca ccgtagtgga attcaatcgg caaccttata
     3301 ttacaggaca gatactttgc aaccttataa tactgtttcg atgaatgcta tatcagggat
     3361 gagtgattac tttgaaacgt acattcctgc acaatctggt ggaacaagaa tttactacta
     3421 tattgaagca tattcaaata cctcaaaaca tcagacacga ccaatgcctg caccgcaagg
     3481 ttatttttat tttgatgtat tacagactac tggaataact gaagttttaa atcagttatc
     3541 ggtgggacaa attttcccaa atcctgcatc ggcagtgaca tgcctcccct actatagtcg
     3601 cattggcaca ttgcttcaga ttaaaatcag tgatgtaaca ggcaagatgg tttatgaatc
     3661 acaaaataca acaaagtcta atggacaaaa ctttgcgttt tttgatgtca gtcaatttaa
     3721 accgggagtg tattttgtaa aatcagaaac taattcatca ataaatattc agaaacttat
     3781 agtaaagtaa ttttatcaga gataattgct gaataacaca attgactatt accttttaac
     3841 tacaattatt ctggtggcaa tattattttt tgatttatca aaaaattgca agttatatac
     3901 accatccttg agattagtca aatctaaact tccatctttc aaattataca tttccttgac
     3961 aatacttcca ttaatgtctg atattttcaa agatgatatc tcggaattat gcagaacaat
     4021 atgaatgatt ccatttgttg ggtttggata aacagaaaat aatcctgctg aaatatcaac
     4081 aataccaact gttccaacgg ttattcttat atcacttaaa acatttgttg cactaagact
     4141 agtaacagaa gaaattggta tagctccata gtaccttctt gatctattag aagaaactga
     4201 tgtgtaatac caacgaggcg acaccggtgt actgagagta gcagtttgtg tatgtttgaa
     4261 caccattacg cttaaattgg atgaggtgct gtaagtaaat gatgtatcta atgcaacctc
     4321 tcgccatcct ataccgccat tattaggaaa tggaccctca taaacaagag tatatccggt
     4381 tgtatcaaca tttcctgcgg ttaacgaagt aaatgctgtc tgtttcataa aaattgagac
     4441 actatcaaac ggtaatgtat cagtaccatc aacacgattg aagccaaaag atgaaatgga
     4501 gccagaatag cctatctcag ttgaaagata tattgctccg taagcaacac attcataaga
     4561 tttgactata ggactaaatg cagaaatcag gcttgatgta ccgacaacaa ctgtttgtgt
     4621 ctgagcattc aaactgccca atgaacataa tgaagccaaa aagaataaaa aaacttttaa
     4681 gtagattttt gtcatcgtaa aataatttag tatttcacga atatacatca aattgattga
     4741 tgttttaaac ttttattttg aaaattatga gagtgtcttc taaagtattt aaaacttata
     4801 ttttgtcaaa tgcattttta gcgctctaag a
//
LOCUS       DAOOOI010000128         4801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3268_length_4801_cov_63.312474, whole genome shotgun sequence.
ACCESSION   DAOOOI010000128 DAOOOI010000000
VERSION     DAOOOI010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3268_length_4801_cov_63.312474"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="PKX59_10495"
     CDS             <1..146
                     /locus_tag="PKX59_10495"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOZ91327.1"
                     /translation="EGDTVADYYLGSGTTAVVCKELNRNFIGCDINPKAIKITNARLD
                     ALS"
     gene            525..1964
                     /locus_tag="PKX59_10500"
     CDS             525..1964
                     /locus_tag="PKX59_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91328.1"
                     /translation="MTARKWVGIESATSVGKTYCLPRIIYWFLDTFPNSLVITTAPKK
                     DQLKKILWTEMGNAYHKFKKIRPHSEFITLNIAVDKRTKKTSTQKVDDSIEGGMGWEV
                     LGIVSGVAAGEDSATKMQGFHRENMLFVIDEMAGVHPAVLTAIINTCTAPNNLIIGVG
                     NPDSQIDTLHTFCQLKKTEHIIVSAYDHPNVVNKRMIIPGAVSVESIDMRKEEYGEES
                     NFYKSRVRGIAPEEGTGSLFRAEYIDQCYIYNKDKFHGNPEKIGNNAVGVDVANSEQG
                     DMACLAWGKGNKLFQLQEFQCDNATHLAYNMMYDTHELARFGYKNYNTLKIKDYKIHD
                     RNIGIDTVGVGVATINAFYDKRKDVVALIGGQLQEALNLDNDGNPLYNFQNLRSQMYF
                     EFREDLRTNGVILDLDKKTFLKLKKELLAHTYKVSGGKIFVESKDDIKKKLGGKSPNL
                     SDSAVYWNFVRKNYYKKTMSLPFSGSAKK"
     gene            1994..4453
                     /locus_tag="PKX59_10505"
     CDS             1994..4453
                     /locus_tag="PKX59_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91329.1"
                     /translation="MGFLDDFGNIVLRYAFGEHKNNQAQTTAKPTVIKAKTEPGFMPV
                     GNVRSSVESGASTFAPTGKLQEIKPDFAIEYLSTIENLAAFNPDISYGLDNIVTLGNT
                     EHDIYFSDKIPESKQSEMRNYLLEQERTWYGFSGGVRSLKGDLLTQIVINGALSAEAV
                     PREDLTAIKKIVRVSPKHIRFIYDKEADEFYPYQNVNILSKASSDYPGKVKLNTTTYK
                     YIAYRRYFETPYAVPPFITAIEALCIQRPMLANFKNIMDKMGMLGFLTATVTSPEQND
                     GEDDTEYWNRCVEYLDKYIYPQMQKNLSSGLVVGFKDAHEFKLEGNNMNVAGADGLVQ
                     LVQLMIFAGLKQDPNMLGRNYSTTETFGRVILEKMLSQIRDYQSLVDQFFSEIYTIAL
                     RLKGYEPGYVKVVSKPPMINDRLKDAQAETAEISNVLAKRNAGIIGQKKAANELGYDE
                     PETEDFEIPGQQTDAGTTPPAEKKKVAPANNSSELIDYYEQKLGVDIPEWSYECNLPH
                     VKKIYNFDGTDFGDKKTNSLSTAYIKETGKVFNKAIDKVSNAVGAKFDKYNENTALND
                     IQNEVWLTILSKWDSNFVVPMNDVIKRNVEAIYNNFRKDKKAFGNTKTTSANQQKFAS
                     ADDIPEAVFSLKDYRTIEYMEQHDNMYLGKFITDKDTKARVYKYIEANYIDGNLPIGK
                     NKSEIQKFIDAFREQLSLEAWKIRRIIDTSVTKLKSFGHLRYLSQAEIEKFEVIELID
                     NVTCPYCEHMDGMEFSVETAVSTIDDEISAGPGKLAATSPFATTYKIDAFKKLTQEDL
                     ESSNIVKPPFHPHCRGRLVSV"
BASE COUNT         1643 a          836 c         1062 g         1260 t
ORIGIN      
        1 acgaaggtga tacagttgct gattattatt taggtagtgg cacaaccgca gtagtgtgta
       61 aagaactaaa cagaaatttt ataggttgcg acattaatcc aaaggctatt aaaatcacaa
      121 atgctcgttt agatgcactt tcgtagcctt gcacataacg tatgagtttg cggtattgga
      181 taaggatgag attttgattt acaaagaaat attcgcatct tcaaagtctg aagcaacaaa
      241 caaattgaaa gcggagaatt atatcctgct gaatgaccta agtaataaaa aacggattga
      301 aagtatagta aatttgctca atgtatttca aaactcgtaa agaattagca ttaatcgaat
      361 tagccaaacg tcgaaagatt ggtaactacc aagaacggcc tgatttatgg ttgagtgaaa
      421 gatttggtga gccattgagc gtactgaagt ggagtgaatt tgagggatat gagaaccata
      481 aatgggacgg caccgtagat ccgttcatgt ctgcctgtaa agcattgacg gcccggaagt
      541 gggttggaat tgaaagcgca acttcagtag gtaaaacata ttgcctacca cgcatcattt
      601 attggttttt agacacgttc ccaaactcgc ttgtaattac tactgctccg aaaaaagacc
      661 aactaaaaaa gattctatgg acggaaatgg ggaatgcata ccataagttc aaaaagatac
      721 ggccacactc cgaatttatt accctgaata ttgcagttga taagcgtacc aaaaaaacct
      781 ccacacagaa agttgatgat agcattgagg gaggaatggg ttgggaggta ttaggtatcg
      841 tttccggtgt ggccgcaggt gaggattcag cgaccaaaat gcagggtttt cacagggaga
      901 atatgttatt tgtcattgac gaaatggccg gtgtgcatcc tgcagtattg acggcaatca
      961 ttaatacgtg tacggcacca aataacctga ttataggcgt aggcaatccc gatagccaaa
     1021 ttgataccct gcatacgttc tgtcagttga aaaaaacaga gcatattatt gtatctgcat
     1081 acgaccaccc gaacgttgtg aataagcgaa tgataatacc aggtgcagta tcagttgaga
     1141 gtatcgacat gagaaaggag gaatacggag aggaaagtaa cttctacaaa agtcgtgtac
     1201 gtggtattgc tccggaggag ggaaccggct cattattcag agcggagtat atagaccaat
     1261 gctacatata caataaggat aagtttcatg gcaatcctga aaagattggt aataatgccg
     1321 tcggagtgga cgtagcaaac agtgagcagg gtgacatggc ttgtttagca tggggtaagg
     1381 gtaacaaatt atttcagttg caggagttcc aatgtgataa cgccacacat cttgcgtata
     1441 acatgatgta cgatacgcat gaattggcac gttttggtta taagaactac aataccctga
     1501 agattaagga ttacaagata cacgaccgta atatcggtat tgatacggta ggtgttggtg
     1561 tagctactat caatgcattc tatgacaaaa gaaaagatgt ggttgcactt atcggaggcc
     1621 aacttcagga ggctttaaat ttggataatg acggcaatcc gctttataac tttcagaact
     1681 tacgttctca aatgtacttt gagttcaggg aagatttgcg taccaatggc gtaattttag
     1741 atctcgacaa aaagacattc ctgaaactta aaaaagaact gttagcacac acatacaaag
     1801 tttccggagg taagatattt gtggaatcaa aggacgatat taaaaagaaa ctcggtggaa
     1861 aatctccgaa cctatcagat agtgcagttt attggaattt cgttcgtaaa aactactaca
     1921 aaaaaactat gtcattaccg ttttcaggta gcgcaaagaa ataatttata cttaacttta
     1981 ggaaaagtat aacatgggtt ttctcgacga tttcggaaat atagtattga ggtatgcgtt
     2041 tggtgagcat aagaataacc aagcacaaac tactgcaaag cctactgtta ttaaggctaa
     2101 gacggagccg ggatttatgc ctgttggtaa tgtcagaagt agtgtagaat cgggtgcctc
     2161 cacgtttgca ccgaccggaa aattgcagga aataaagcct gattttgcta ttgaatatct
     2221 atctaccatt gaaaatttgg ccgcattcaa tccggatatc tcttatggat tagacaacat
     2281 tgttacgttg ggtaatacag agcacgatat ttacttctcc gataagatac ctgaaagtaa
     2341 gcaatctgaa atgcgtaatt acctattaga gcaggagcga acgtggtacg gatttagtgg
     2401 aggagtgaga agtttgaaag gagatttact cacacagatt gtgataaatg gtgcactatc
     2461 tgcagaggcc gttccacgag aagatttaac tgccattaag aaaatcgttc gtgtatctcc
     2521 aaagcacata cgtttcatat acgacaaaga ggccgatgaa ttttatccgt atcagaatgt
     2581 aaatattttg agtaaagcga gtagtgatta tccaggcaaa gtaaagctga atacgaccac
     2641 atacaagtac attgcttacc gcagatactt tgaaactccg tatgcagttc ctccgtttat
     2701 tacggccatt gaagcgttat gcattcagcg tccaatgtta gccaacttca aaaacatcat
     2761 ggataaaatg ggtatgctcg gttttcttac tgcaacagta acgtcaccgg agcagaacga
     2821 cggagaggac gataccgaat attggaaccg ttgcgttgag tatttggaca agtacattta
     2881 tccgcagatg cagaagaatt tatcttcagg attagttgta ggatttaaag atgcacatga
     2941 gttcaagttg gagggaaaca atatgaacgt ggcaggagcc gacggattgg tacagttagt
     3001 tcagttaatg atatttgcag gtttgaaaca agatccgaat atgttaggcc gtaactattc
     3061 cactaccgaa acgttcgggc gtgttatcct cgaaaaaatg ctatctcaaa tcagagatta
     3121 tcaaagttta gtggaccaat tctttagcga gatttacacg attgcattgc gtttgaaagg
     3181 ctatgaacct gggtatgtga aagtagtcag taaacctccg atgattaatg acaggctgaa
     3241 agatgcacag gctgaaactg cagaaatatc gaatgtattg gcgaaacgta atgccggtat
     3301 cattggacaa aagaaagcgg ccaatgagtt agggtacgat gagccggaaa cagaagattt
     3361 cgaaattccc ggacaacaga cagatgcagg taccactcca cctgcagaaa aaaaaaaggt
     3421 agcaccggcc aataacagta gcgagttaat cgattactat gaacagaaat taggagtgga
     3481 tattcctgaa tggagttacg aatgcaacct gccacacgtt aagaaaatat acaactttga
     3541 cggaacagac tttggtgaca agaaaacgaa tagcctatct actgcataca tcaaagaaac
     3601 aggcaaggta ttcaataaag ccattgataa agtttccaat gccgtaggag caaagtttga
     3661 taagtacaat gaaaatactg ccttaaacga tatccaaaac gaagtatggt taacaatact
     3721 gtcaaagtgg gatagcaact ttgtggtacc aatgaatgac gttatcaaac gtaacgtgga
     3781 ggcgatatac aacaacttca ggaaagacaa aaaagcattc ggtaatacta agactacatc
     3841 tgccaaccaa cagaagtttg catctgcaga cgatattcct gaagcagtat tctcgctgaa
     3901 agattatcgt accattgaat acatggagca acacgacaat atgtatttgg gtaagttcat
     3961 tactgataaa gataccaaag ccagggtgta taaatacatt gaggccaact acattgacgg
     4021 caatctgcct ataggtaaaa acaaatcaga gatacagaaa tttatcgatg cattccggga
     4081 gcagttatct ttagaggctt ggaagattcg acgtattatt gatacttcag taacgaaact
     4141 aaaatcgttc gggcacctgc ggtatctttc acaggccgag atagaaaaat tcgaggtgat
     4201 tgaattaatc gacaacgtta cgtgcccgta ttgcgaacac atggacggca tggagttcag
     4261 cgtcgaaacg gcagtcagca cgatagacga tgagatcagt gcaggtcccg ggaagttggc
     4321 agcaacgagt ccgtttgcga cgacgtataa aatcgatgcg ttcaaaaaat taacgcagga
     4381 agatttggag agtagcaata tcgtaaagcc tccgtttcat ccgcattgcc gaggccgttt
     4441 ggtatctgtc taatttcctg cactccacaa aaaaaagaga gatataagat gtagtattca
     4501 gaactttcgt ttttagaaat attattcaaa acatgataga gcatttttga acctgaaata
     4561 tggtacaaaa atgctttttt tattttcctt aacaatttag taacagttta taacgagttg
     4621 aaataagcgt acttatgatt ttattttgaa aataatggta ttataatagt gcaggatatt
     4681 gcacaaagca aatatcctgc atagatttac actatcaaat tgaaataaca acaaaaataa
     4741 acactttaaa aaaacagcaa ttatgagaac atttaaagta aacttcaaaa cagcatccgg
     4801 a
//
LOCUS       DAOOOI010000129         4760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3294_length_4760_cov_9.611265, whole genome shotgun sequence.
ACCESSION   DAOOOI010000129 DAOOOI010000000
VERSION     DAOOOI010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3294_length_4760_cov_9.611265"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="PKX59_10510"
     CDS             <1..1189
                     /locus_tag="PKX59_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91330.1"
                     /translation="YYSGGDTIYYFFDSMTMSNLLFKNFVQFGDVVINGWANHKFSYL
                     DSTTGTFVYYRSPESFFISRIIWPFTIIGLQQMIPTVMVLALVSFGGVWRMYQIFIKE
                     FPALERPLAYTFFFLPSVFFWGSGILKDSITLSALGYFFYSFYYVFIEPKKVIKNLII
                     VVISAWIIISIKAYIFIGVLPGALIWIVGKMTSRIRGSFIRKVTFPLIMIIVGFSGFL
                     LLNMLGDELGKFKIDKLLDEAVVSNTDLQADYYGGNSFSIGEIEPTVESMLIHMPLAI
                     NAALFRPYITEARNVMMMVSGIENLFILFFTLYVLYKVKIFGIFKYFNKNHLLTFSLI
                     FSLFFAFAVGISTSNFGSLVRYRIPLLPFYVSSLVIIRYMYEKEKVEKRELSYSYYKA
                     ITE"
     gene            1817..>4760
                     /locus_tag="PKX59_10515"
     CDS             1817..>4760
                     /locus_tag="PKX59_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91331.1"
                     /translation="MIRQLLTTVVFFIFSFSSIAQNYLMSSTNVSTCAGNFYDSGGGG
                     ANYGLNQNTTMTLCSNTAGQCIRISFTAFNLENNFDFLRIYNGPNTASPLIGSYTGTT
                     SPGVVTGTSGCLTFVFTSDGSINHAGWTAAISCVTCAAGGSCLQTCNGGPAPANDACS
                     GAQNLGMLPAPAACPNGIGSWVNVSTTNLCATAEMPYTTLTGCNPSGNMASPASDVWY
                     RFNLTGPTLNVQISGGIQTPNVALYSGNACNNLIGRGCAIGAGGILNTTFGGLAAGTY
                     YLQISGGSLTDQCDFNLALQNNFDCQGCVIVNNLVVNPPPVNGTYQAGQLVNFCYTIS
                     DYNQTSVNWLHAVVPTFGAGWDMSTLTTVMPANCSGSGSWGWYNTNITSSATGVSHGP
                     GFYYESGLGSPLGFMDGNPGNNFGDNNALNLCNWTFCFSVRTMPASQCTQGASLNVSI
                     DTYGDGESGSWTSLACTGDPVTDFFATLSCCQPPIVNPNNPLCDGYVGSANGIAQGAS
                     PWTYIWQNSSGGTIMQVNNINSAHAIPNLATGNYSLTTIDNNGCSSTTYFSITTPPAI
                     VIAANAVGTKCNNSDGAINIFASGGVSPYQYSINNGSTFSATSVFNNLAAGSYTVVVK
                     DANGCTSSQVVNVAPSSSPVINSSPVTQVSCFGGNNGSIVVNATGGNPPLQYSIDNGT
                     TYQASNSFAALTAGTYTVVVSDVDGCTYSVTLNVTQPAQINISNVQTTDATCGNSNGS
                     LVVTASGGTGTLQYSITGGAPYQASNTFNNLAASNYNVVVVDANGCTQTINAQVNNSS
                     APVINTTNATSLLCNGDNNASITISASGGTGTLQFSINNGATFQSGNVFNGLAAGTYN
                     VVVSDAVGCLVSTSIVIADPPILNISASNVGTTCTQSNGSISASGSGGTGVLQYAING
                     GSYQASTNFNNLAAGNYTVTVQDANGCTASTTTNITDAPSAVIVNITTTPVNLSLSSP
                     VLSSSGVQA"
BASE COUNT         1432 a          817 c          930 g         1581 t
ORIGIN      
        1 ctattattca gggggtgata ctatttacta tttctttgac tcaatgacaa tgtccaatct
       61 gttatttaag aattttgttc agtttggtga tgtagtaata aatgggtggg cgaatcacaa
      121 attcagttat ttggatagta ccacaggaac atttgtttat tatcgtagtc ctgagtcatt
      181 ttttatatca agaataatct ggccatttac aattattgga ttgcagcaaa tgatacctac
      241 tgttatggtg cttgcattgg tttcttttgg tggtgtttgg cgtatgtacc agatatttat
      301 aaaagaattt cctgcattag aacgaccact tgcctacaca tttttctttt tgccttcggt
      361 atttttctgg gggtctggaa ttttgaagga ttcaattaca ttaagtgcat tgggatattt
      421 cttctattca ttttactatg tatttattga accaaaaaaa gttattaaaa acttgattat
      481 tgttgtgatt tctgcatgga ttatcatttc aattaaagca tatattttta ttggagtgct
      541 gccgggggca ctcatctgga tagtagggaa aatgacgtca agaattcggg gaagttttat
      601 cagaaaagtt actttccctt tgataatgat tattgtcggt ttttccgggt ttttattact
      661 caatatgttg ggtgatgaat taggtaagtt taaaattgat aaacttttag atgaagcagt
      721 tgtatcgaat accgatttac aggcagatta ctacggtggt aactctttta gtataggtga
      781 aattgagcct acggttgaaa gtatgttaat tcatatgcca ttggcaataa atgcagcatt
      841 attcagaccc tatattactg aagccaggaa tgttatgatg atggtgtccg gaatagagaa
      901 tttgtttatt ctgttcttta cattatatgt gctttataaa gtgaaaattt ttggaatttt
      961 caaatacttt aataaaaatc acttactgac tttttctctt atattcagtt tgttttttgc
     1021 ttttgctgta ggtatttcaa ccagtaattt cggtagtctt gttaggtata gaattccatt
     1081 actgcctttt tatgtttcat ctttagtaat catcagatat atgtatgaaa aagaaaaagt
     1141 tgaaaaacgt gagttaagtt atagctacta taaggcaata acagagtagg ctttaattta
     1201 ttttatccga aaaattaaca ggagtttcat ttgatatctg aggtttcagg aaatgaacaa
     1261 ttatttattt tctgaaaaga aggatgcctg cctaaaagaa aatgactaac caaaaccgaa
     1321 ttctaaatca gacaggcgct ccttatgtgc gcttttcata gctgcttaca tggcaaaaat
     1381 tccatcatgg cttcggaact atgcagtaag agcatgaatt agtcgaaaag ttgcatagcc
     1441 cttgaatttt ttcttttcaa atcactaata cattgaattg cagtaggtaa tttatggcct
     1501 aattggggtc aaaaggtaca atgtaaagat ttatataatg tttgtggaaa ataatttcta
     1561 atgcactgat taaaattatt tgtttttatg aaaattacat taaatttacc ccgactaagt
     1621 tagccaccac taaagcacca aagactacag atatcagccc atagcgaata actttttaaa
     1681 ttgtttcgaa tggttttaaa gaataagctg agtagtattt tcaatgttga ttctcgttcc
     1741 ggtttattgc cgaagcagga atcaaatatt cccataataa accaccaacc taagtttcat
     1801 ttaatcagcc tggctcatga ttagacaatt actcaccacc gttgtttttt tcattttttc
     1861 attttcgtca attgctcaga attatttaat gagcagcacc aatgtttcaa cctgtgcagg
     1921 gaatttttat gatagtggag gtggaggagc caattacggc ctcaaccaaa acactactat
     1981 gaccttatgt agtaacacag ccggacaatg catcaggatt tcttttactg cttttaatct
     2041 tgaaaataat tttgattttt taagaattta taatggccca aatacagcat caccattaat
     2101 cggttcctat acaggaacga caagccccgg tgttgttaca ggaacttccg gctgcttaac
     2161 ttttgttttt acctcagatg gttcaataaa tcatgcagga tggacagcag ctataagttg
     2221 tgttacctgt gctgcaggtg gtagctgtct tcagacttgt aatggtggtc cggcacctgc
     2281 aaatgatgca tgtagtggag cgcaaaacct tggaatgctt cctgcacctg ctgcatgccc
     2341 taatggaata ggatcgtggg taaatgtcag tactaccaac ttatgtgcta ctgctgaaat
     2401 gccttatacg actttaacag gatgtaaccc ttcaggtaat atggcaagcc ctgcaagtga
     2461 tgtatggtat cgttttaatt taacaggtcc tactttaaat gtgcaaatct caggcggaat
     2521 acaaacaccc aatgtggcac tttattcagg caatgcatgc aataacctta ttggtcgtgg
     2581 atgtgcaatt ggcgcaggtg gtattctgaa tacaacattt ggtggtcttg cagcaggaac
     2641 atattatttg cagatcagtg gaggtagttt gacagatcaa tgtgatttta atctggcgtt
     2701 acaaaataat ttcgattgtc agggttgtgt tattgtaaat aatttagttg tgaatccgcc
     2761 accggttaat ggaacgtatc aagccggaca attagttaac ttctgttata ctatttctga
     2821 ttacaatcaa acttctgtta attggcttca tgccgtagtt ccaacctttg gtgcaggatg
     2881 ggatatgagc acattaacta ctgttatgcc ggccaactgt tccggttccg gttcatgggg
     2941 ttggtataat actaacatta caagttctgc caccggtgtt tcacatggtc ccgggtttta
     3001 ttatgaatca ggtttaggaa gtcccttagg ttttatggat ggtaatcccg gaaataattt
     3061 tggggataat aatgcattga atttatgtaa ctggactttt tgttttagtg tgagaacaat
     3121 gcctgcttca caatgtacgc aaggtgctag tttaaatgtc agtatagata cttatggtga
     3181 tggagaatca ggctcatgga catcattagc ttgcacaggt gatcctgtta ctgatttttt
     3241 tgcaacgctt tcttgctgtc aacccccaat tgtaaatcca aacaacccgc tttgtgatgg
     3301 ctatgttggt agcgcaaacg gcatagctca aggagcatct ccttggactt atatctggca
     3361 aaacagttcc ggtggaacaa ttatgcaggt caataacatc aatagtgctc atgctattcc
     3421 caatcttgca acaggaaatt attcactcac aactattgat aataatggat gttcatcaac
     3481 aacttacttt tctattacaa caccacctgc aattgttata gctgccaatg cagtcggaac
     3541 aaaatgtaat aatagtgatg gcgcaataaa tatatttgca agtggaggag tttcaccata
     3601 ccaatatagc atcaataatg gctcaacatt cagtgccaca tctgtattta acaatcttgc
     3661 tgcaggaagt tatactgtag ttgtaaaaga tgctaatggc tgcacatcat cgcaggttgt
     3721 taatgtagca ccttcatcaa gtcctgtaat aaacagtagt cctgtaacac aagtcagttg
     3781 ctttggtggt aataatggaa gtatagttgt taatgcaacc ggtggaaatc cacctttaca
     3841 atatagtatt gataacggta ccacttatca agcttcaaat agttttgcag cattaactgc
     3901 aggaacttat accgttgttg ttagtgatgt agatggctgt acgtatagtg taacactcaa
     3961 tgttacacaa cctgcacaga taaatatatc caatgttcaa acaacagatg caacttgtgg
     4021 caacagtaat ggatcattag ttgttactgc aagtggcgga acagggacat tacaatatag
     4081 cataacaggt ggggcacctt atcaggcatc aaatacattt aataatcttg ctgcatcaaa
     4141 ctataatgtt gtagttgttg atgcaaatgg ttgtacacaa acaatcaatg cacaggtaaa
     4201 taattcatct gcacctgtta taaatacaac taatgcaaca tcactattat gtaatggcga
     4261 taacaatgct tcaattacaa tcagtgcaag cggtggaacc ggtacactgc aattcagtat
     4321 taataatggt gcaacttttc aatcaggaaa tgtttttaat ggattagctg caggcacata
     4381 caatgttgtt gtcagtgatg cagtaggttg tctggtttca acttctatcg ttattgctga
     4441 ccctccaata ttaaacatat cagcaagcaa tgttggtaca acatgtacac aaagtaatgg
     4501 ttctattagt gcaagtggta gtggtggaac aggtgttttg caatatgcaa taaatggtgg
     4561 ttcctatcag gcgtcaacaa actttaataa ccttgctgca ggtaactata ctgtaactgt
     4621 tcaggatgca aatggatgta ctgcaagtac aacaaccaat atcaccgatg ccccttcagc
     4681 agtaattgtt aatataacta caacaccggt aaatttatct ctgtcatcgc ctgtgctatc
     4741 atcatcgggt gtacaggctg
//
LOCUS       DAOOOI010000130         4728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3316_length_4728_cov_15.312861, whole genome shotgun sequence.
ACCESSION   DAOOOI010000130 DAOOOI010000000
VERSION     DAOOOI010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3316_length_4728_cov_15.312861"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PKX59_10520"
     CDS             complement(<1..296)
                     /locus_tag="PKX59_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ91332.1"
                     /translation="MKSHHYNFPIAPFNEDMLLIDFFDIQASKTPDQIAVYFHEEKLT
                     YAQLEKQAGILAQFLINKGVKPGDLVGLCIERSCNLIVGVLGILKAGAGYVPLDP"
     gene            complement(510..1172)
                     /locus_tag="PKX59_10525"
     CDS             complement(510..1172)
                     /locus_tag="PKX59_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOZ91333.1"
                     /translation="MTDFVPSLRFHFLTPFYDFLFGILLPEKKLRNTIISIIETTNND
                     SLIELGCSTGGLTLPIAKRFSSSRIYAVDIDKKALSILERKLNKVPQHKISLINASSS
                     LLPLENKSVPTIITSLLFCNLLPEEKLKTLHEIKRILTDNGHLIIMDWGKPKTIFSAA
                     GFKILQWVGGHKTTDDLKQGLFKVLVQNQGFTITEKKYINTGFGTLYFYDAMPDSQRN
                     LP"
     gene            complement(1184..1930)
                     /gene="fabG"
                     /locus_tag="PKX59_10530"
     CDS             complement(1184..1930)
                     /gene="fabG"
                     /locus_tag="PKX59_10530"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOZ91334.1"
                     /translation="MKLLENKVVLITGASRGIGRGIALTLAQHGANIAFTYLSSPEKG
                     MELENILKSHGVKAKGYQSDASQFNAAEQLINQVLTDFEKIDILVNNAGITRDNLLMR
                     MTEEQFDEVLRTNLKSCFNTTKHIQKSMLKQRSGSIINITSVVGEKGNAGQANYAASK
                     AGMIGFTKSVALELGSRNIRCNAIAPGFIETEMTGSLDPATVKGWADAIPMKRGGTVD
                     DIANAVLFFASDLSTYITGQVLHVNGGMYT"
     gene            2003..2719
                     /locus_tag="PKX59_10535"
     CDS             2003..2719
                     /locus_tag="PKX59_10535"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOZ91335.1"
                     /translation="MKKLLFSVALVAMALTVNAQKFKVGLGGGFNSTWLMNKNVFDAD
                     DDLNVAMSFGGQFGLKTEYYFNDKLALELGIMRAGHNQKYDLNDGHDQLTLKLRYLDV
                     PLLLRLGGDKGGYFEFGPQFSFLSSAKDVDDDGDKHDVKGSLKGANIGLVIGFGVDVD
                     LSEQFTLTAGMRLGYGFSDVTKKYSNAIDMAQDMDDMSGATYWANFEDGTSGNFSYKT
                     TSRVYGGIHLGVLYTIPTGK"
     gene            2832..4658
                     /gene="asnB"
                     /locus_tag="PKX59_10540"
     CDS             2832..4658
                     /gene="asnB"
                     /locus_tag="PKX59_10540"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOZ91336.1"
                     /translation="MCGIAGFIDDIADGEKILSAMLKAIAHRGPDASKMYMSQNVHLG
                     HNRLSIIDLSSNADQPMYDNGLTIVYNGEIYNYIELRAELEKNGEVFKTKSDTEVILK
                     AYQQWGEKCVEHFVGMWAFALWDQKENKLFCSRDRFGIKPFYYISNNNKFYFGSEYKA
                     LKPTPVFTNEINQYQVLRGLQMGWICYEDETYFDIIKALPAAHNLVYYRDKAEIKIYR
                     YWSLQTGVYSNLSFDEKCATFYELFSQSIKLHYRSDVPVASCLSGGLDSSAIVSMVQE
                     QHPGMSYKTFSIYYEGKGDVDERPFVNEVLKKYPALKPYYFSPSDSQVTEHFHHALYH
                     ADVPSTGSSFISQYFLMKLIKEQGIKVVLDGQGADEYLAGYKHTFYRMVADILSSGQV
                     SQALALTGIVAKNQELSSTQQLAHLGKSLLSVAKREQKLYALEYLKYYPFMGKDKHVP
                     FWLDKVDGNRTDNFLYHLLFNTSLPSLLQYEDRNSMAFSIESRVPFLDHRLVEFSFGL
                     RNEEKANGIYTKWIMRSALQNVLPPKIAWRKDKKGFVTPGEVKWLRGPLAHLIESDFN
                     KLDFLNQHKVKRIITDYKNGDNSQALLVWRLCTLAYWQKHFC"
BASE COUNT         1454 a          789 c          950 g         1535 t
ORIGIN      
        1 gggtcgagag gaacatatcc ggcaccggcc ttcagaatcc ccaatacacc aacaataaga
       61 ttacaactgc gttcaataca aagaccaaca agatcacctg gttttacacc tttgttgatc
      121 agaaattgtg caagaattcc ggcttgtttc tctagctgtg cgtaagttaa tttctcctca
      181 tgaaaatata cggcaatttg gtcaggtgtt ttagaggcct gaatatcaaa aaaatcaatc
      241 agcagcatat cctcgttaaa gggtgctata ggaaaattgt aatggtgcga tttcattaag
      301 ttggtggttt aaaaactatt atgtgccgcc cttcggtaca actatattgt acggaagcga
      361 atataataag tttcacttta tgaagtatct gtttttggtg gatgcaccag attgtgaatt
      421 gtagcttgaa aatgcggttg taaaaaagca gtgttaggag ttaaaataca gtaataagca
      481 ggattattcc ctgttctgaa tatttaatac taaggtagat ttctctgaga atcaggcatt
      541 gcatcataaa agtaaagcgt tccaaagccc gtattgatgt attttttttc tgtaatggta
      601 aatccctgat tttgcaccaa tactttaaaa agcccttgtt tcaaatcatc agttgttttg
      661 tggccaccaa cccattgcaa tatcttaaaa ccagctgctg aaaaaatggt tttcggtttt
      721 ccccaatcca taataatcaa atgcccgtta tcagttaaaa ttctttttat ttcatgtaaa
      781 gttttaagct tttcttccgg aagtaaattg cagaacaaca ggcttgtaat tatagttgga
      841 acacttttgt tttccaatgg caaaagagaa gaagatgcat taatcagcga aattttatgt
      901 tgtggtactt tattcagttt tcgctctaat atggacagtg cttttttatc aatatctact
      961 gcgtaaatcc gtgaagagga gaatcttttt gcaataggta gggtaagacc gcctgtgctg
     1021 catcccagtt caattaaact atcattgttt gttgtttcaa tgatactgat aatggtattt
     1081 cgtaattttt tttctggcag caatatgcca aaaagaaaat cataaaatgg tgtaagaaaa
     1141 tgaaaacgta gtgatggaac aaaatcggtc acatcatcaa ttattaggtg tacattcctc
     1201 catttacatg cagtacctgt ccggttatat aggtacttaa atcagatgca aaaaataaga
     1261 cagcattagc aatatcatca acagttccac cgcgtttcat gggtattgca tcagcccatc
     1321 cttttacagt tgcagggtct aaagagcctg tcatttctgt ttcaataaaa cccggggcta
     1381 ttgcattgca acgaatatta cgggagccta attctaatgc tacagacttt gtaaaaccta
     1441 tcatccctgc tttgcttgca gcatagtttg cttgtccggc atttcctttt tcgccaacaa
     1501 cagacgttat atttataata gaacctgaac gttgcttaag cattgatttc tgaatatgtt
     1561 ttgtggtgtt aaaacaagat ttcaggttgg tgcgaagtac ttcatcaaat tgctcttctg
     1621 tcattcgcat tagcagatta tcacgtgtta tacctgcatt gtttaccaga atatcaattt
     1681 tttcaaaatc tgtaagaacc tgattaatca gttgctcggc agcattaaac tgcgatgcat
     1741 cactttgata acctttagct tttacaccat gtgattttaa aatattttcc aattccattc
     1801 ctttttcagg cgatgacaag taggtaaagg caatatttgc accatgctgt gccaacgtaa
     1861 gggcaatacc tcttccaata ccgcgcgaag caccggttat caatacaact ttgttttcga
     1921 gtagtttcat accattagat tttgacaatt gaattaaagt tctacatttg cggttcaaat
     1981 atatagctaa actttaaaag tgatgaaaaa actattattc agtgttgctt tagtagcaat
     2041 ggcgttgaca gtaaacgcac aaaaattcaa agttggttta ggtggtggct ttaacagcac
     2101 ctggctgatg aataaaaacg tattcgatgc cgatgatgat ttaaatgttg ccatgtcttt
     2161 tggcggtcaa ttcgggttga aaacggagta ctattttaat gataaattgg ctcttgaact
     2221 gggcattatg cgtgccggcc acaatcagaa gtatgatttg aatgatggtc atgatcaact
     2281 tacattgaaa ttacgttatt tagatgttcc tttattactg cgtttgggtg gtgacaaagg
     2341 aggatatttt gaatttggac ctcagttcag ttttcttagt agtgccaagg atgttgatga
     2401 cgatggcgac aaacatgatg tgaaaggatc acttaaaggt gccaatattg gtcttgtaat
     2461 aggctttgga gttgatgttg atttgagcga gcaatttaca ttgactgcag gtatgcgttt
     2521 gggttacggt ttcagtgatg ttactaagaa gtattctaat gctatagata tggcacaaga
     2581 tatggatgat atgtcaggtg ctacttattg ggctaatttt gaagatggaa catcaggtaa
     2641 tttcagctat aagactacat caagagttta tggtggtatt catttaggcg ttctttatac
     2701 aattccaaca ggaaaatagt agaatcaact atttataaaa ttgaccgctt caacattgga
     2761 gcggtttttt tatggattca tggtttattt atttttgaca aaaattttta attttttgta
     2821 agaagcataa aatgtgtggc atagccggat ttattgatga tattgctgat ggcgagaaaa
     2881 ttctttctgc tatgcttaaa gccattgcac accgtggacc tgatgcaagc aaaatgtaca
     2941 tgagtcagaa tgttcatctc ggacataaca gactcagtat aattgacctt agttccaatg
     3001 ctgatcagcc gatgtacgat aacggtctga ctatagttta caatggagaa atttacaatt
     3061 acattgagct tcgagcagaa ttggaaaaga atggtgaggt ttttaaaaca aaaagcgata
     3121 ctgaagtaat cttgaaagcc tatcagcagt ggggagaaaa atgtgttgaa cattttgttg
     3181 gcatgtgggc atttgcctta tgggatcaga aggaaaataa acttttttgt tcgcgtgaca
     3241 gattcgggat aaaaccattt tactacataa gcaacaataa taaattttat ttcgggtcgg
     3301 agtataaggc attaaaacct actcctgttt ttacaaatga aattaatcaa tatcaggtgt
     3361 tgcgtggttt gcaaatggga tggatatgct atgaggacga aacctatttt gacatcatca
     3421 aagcattgcc tgctgcacat aatctggtat attatcgtga taaagctgaa attaaaatct
     3481 atcgttactg gtcgttacaa accggagttt attccaacct gagttttgat gaaaagtgtg
     3541 ctacatttta tgagttgttt tctcagtcaa taaagttaca ttatagaagt gatgtgcctg
     3601 tggcaagttg ccttagtgga ggattagaca gcagtgccat tgtaagcatg gtgcaagaac
     3661 agcatccggg aatgtcctac aaaacattca gcatttatta cgaaggtaaa ggcgatgtag
     3721 atgagcgtcc atttgtgaat gaagttctga aaaaatatcc tgctctgaaa ccttattatt
     3781 tttcaccatc tgatagtcag gtgacggaac attttcatca tgctttgtat catgctgatg
     3841 ttccaagtac gggctcctct tttatctcgc aatattttct gatgaagctg attaaagagc
     3901 aggggataaa agttgtgtta gatggacagg gagcagatga atatctggct ggatataaac
     3961 atacttttta tagaatggtt gccgatatac tctcgtcagg acaagttagt caggcattag
     4021 cacttacagg cattgtggct aagaatcagg aactgtcttc tacgcagcaa ttagctcatt
     4081 taggtaaaag tttattaagt gttgctaaac gtgaacaaaa attatatgca cttgaatacc
     4141 tgaaatatta tccgtttatg ggtaaagaca aacatgttcc attttggtta gacaaggttg
     4201 atggcaacag gacagataat tttttatacc atcttctttt caatacaagt ttgccatcac
     4261 tgctgcaata cgaagaccgt aacagcatgg ctttctccat tgaatcgcgt gtgccgtttt
     4321 tagatcatcg cttggtggaa ttttcttttg gattaaggaa tgaagaaaaa gccaatggca
     4381 tatacaccaa atggataatg cgtagtgcct tacagaatgt attaccacct aaaattgcct
     4441 ggcgtaagga taagaaaggt tttgtgacac ccggtgaggt gaaatggcta agaggccctt
     4501 tagcacattt aatagaaagc gattttaata aacttgattt tctgaatcaa cataaagtta
     4561 aacgtataat taccgactat aaaaacggtg ataacagtca ggcattatta gtgtggcgac
     4621 tttgcacttt agcctactgg caaaaacact tctgttgatt tttacaaatc gtttgctttt
     4681 tcaatcaatt cagcaatgtc aagaactttt acagaatctt ctttgttg
//
LOCUS       DAOOOI010000131         4726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3320_length_4726_cov_18.142368, whole genome shotgun sequence.
ACCESSION   DAOOOI010000131 DAOOOI010000000
VERSION     DAOOOI010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3320_length_4726_cov_18.142368"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PKX59_10545"
     CDS             <1..281
                     /locus_tag="PKX59_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91337.1"
                     /translation="ITGINEIQSSAISVYPNPATDFINVALPESQTETSYSILDLSGK
                     IVDQGKMVIPQGKSFSVNLSQSLKEGIYFIEVSNGVDSYKHKFVKIKN"
     gene            complement(311..1609)
                     /locus_tag="PKX59_10550"
     CDS             complement(311..1609)
                     /locus_tag="PKX59_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOZ91338.1"
                     /translation="MMKKLLTLLFAVVILPVVALSQSPALVGYWHNWDITAAPYIMLD
                     SVDSRYNIIDIAFAVPQAGTDYQMEFIPEQVTPAAFATQVQNLQNQGRKVIISMGGAT
                     APVSLDNAVERDSFVASMTSIINFYGFDGIDIDFEGSSISVSGGTIANPIDAKIINLI
                     DAVKQIMSNYYATHGKKMILTMAPETAFVQGGMSAFGSIWGAYLPVIDALRDSLEILH
                     VQLYNSGTMFGIDGNIYTQGTADFIVAMTEAVIQGFNTSGGLFAGIPANKVAVGLPAC
                     TNAAGGGYTDTATVKSALDYLLGKGPKPGTYTLVQSGGYPTLRGMMTWSINWDAVNNC
                     STSSYQYAENFQKIFGTSTSLADVNQQRFSVFPNPASDKIYISTKSEGSKRFFIYDML
                     GKMIMSDNYSSTKEEIDISSLSNGVYYVKLENRIFKFVKY"
     gene            complement(1666..2199)
                     /locus_tag="PKX59_10555"
     CDS             complement(1666..2199)
                     /locus_tag="PKX59_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ91339.1"
                     /translation="MRKIHKIAIVGPESTGKSLLSEQLSNHYNTAWVPEVARDYLHKL
                     NRPYTFDDLSVIAKLQLKEEERLSKTAKHFLFCDTNLIVIKVWSDFKYGRTEKWIVDA
                     IQKSDYSLHLLTDIDLPWEDDPLREHPHFRSQLFELYLQELNSFGLEYQTVRGIGEDR
                     IKNALNHIEKLEPNLIR"
     gene            complement(2183..2782)
                     /gene="pnuC"
                     /locus_tag="PKX59_10560"
     CDS             complement(2183..2782)
                     /gene="pnuC"
                     /locus_tag="PKX59_10560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOZ91340.1"
                     /translation="MDLQTIMFLNIPLIEWIGFLFGVVGIYLGIKEKIAYFPISLINV
                     IVSLYIFYHQSLYSDVVQQGVYILLLSYGWYRWIKGSQNTEMPVTNASKKLIFILLGG
                     IILLTVGMGGWFSHYTDAHLPYWDALATSMCFAAQYLMAEKKIENWLVWIDANILYIG
                     IYLYKGLPLYAILFSIYLIMAIVGYFKWQNHLIRHEENS"
     gene            complement(2786..3538)
                     /locus_tag="PKX59_10565"
     CDS             complement(2786..3538)
                     /locus_tag="PKX59_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOZ91341.1"
                     /translation="MKQQILQQFHNHKKQLAVLIDPDKVNEDQLKKLCAYATEHQVDY
                     FFVGGSILTHGSISETIARLKKFASLPVILFPGNASQVDPQADAILYLSLISGRNADL
                     LIGQHVLSAMSVKLSGLEAIPTGYILIDSGNVTTVQYVSNTKPIPHDHSELAVSTAVA
                     GQLLGMQVIYLEAGSGARRTVSGQMIELVKKNTGLPLIVGGGIRTAEKAIEIYNSGAD
                     IIVVGNAIEDNPELMIEIAKAKTTINQTLNAL"
     gene            complement(3541..4170)
                     /locus_tag="PKX59_10570"
     CDS             complement(3541..4170)
                     /locus_tag="PKX59_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOZ91342.1"
                     /translation="MPLIKSTKSDNSFTGIWHLTESTNMLSQMLRFYGKYNLPGHYTH
                     QRRIAEWYAVRLILHEILPDKHFDIFYNEFGKPFLASPEGFISISHTHGFVGVLFHRF
                     NNCGFDMELMDRPVASISHKFVRSDEESFLKDNPLQKTLMLWSAKEVIYKIYGKKSLD
                     FKDNLRILPFEYQHQGEIKGQLILNNDICNYDIQYSFQDQLLTTFAIEK"
     gene            4250..>4726
                     /locus_tag="PKX59_10575"
     CDS             4250..>4726
                     /locus_tag="PKX59_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOZ91343.1"
                     /translation="MSTVTDSKLKYKVKDISQAEWGRKEIKLAEAEMPGLMSLRAEFG
                     KQKPLKGARIAGCLHMTIQTAVLIETLKELGAEVTWSSCNIFSTQDHAAAAIAAAGIP
                     VYAWKGMSNEEFDWCIEQTLFAFEGGKPLNMILDDGGDLTNMVFDKFPELIKDIKGL"
BASE COUNT         1561 a          906 c          806 g         1453 t
ORIGIN      
        1 ctattacagg aataaatgaa attcaatcat ctgccatttc tgtttatcct aatccggcaa
       61 cagattttat caatgtagca ttgcccgaaa gtcaaactga aacatcctat tcaattcttg
      121 acttgtcagg taaaattgtt gaccaaggaa agatggttat tccacaaggg aaaagctttt
      181 ctgttaatct ttcccaatca ttgaaagaag gaatttattt tattgaagtc agtaatggcg
      241 ttgacagcta taagcataag tttgttaaga tcaagaacta attagcccta acaaagggtt
      301 aaaaagaggc ttagtacttt acaaatttaa aaatccgatt ctctaattta acataatata
      361 ctccatttga taaggaagaa atatctatct cttcttttgt tgacgaatag ttatcagaca
      421 taatcatttt accaagcatg tcataaataa aaaatcgttt gctaccttct gacttcgttg
      481 agatataaat tttatcagac gcaggatttg ggaatacaga aaaacgctgt tggtttacat
      541 cagctaatga agtgcttgtt ccaaatattt tctggaagtt ttctgcatat tgataagacg
      601 aagttgagca attatttaca gcatcccaat tgattgacca tgtcatcata ccacgtaaag
      661 ttggatagcc accagactga accaatgtgt atgttccagg ttttggccct ttgccaagta
      721 aataatctag tgccgattta acagttgcag tatcagtata accacctcct gctgcatttg
      781 tacatgccgg caatcccact gccactttat tagccggtat gcctgcaaac aaaccaccag
      841 atgtattaaa cccttgaatg actgcctctg tcattgcaac aataaagtcg gcagtgcctt
      901 gtgtgtaaat gttaccatca atgccaaaca tagttccgct gttgtaaagc tgaacatgta
      961 atatttctaa cgaatcgcgc aaagcatcaa tgacaggtaa ataggcaccc caaatactac
     1021 cgaaggccga catgcctcct tgcacaaatg cagtctcagg tgccatggta agtatcattt
     1081 tcttgccatg agtagcataa tagttgctca tgatttgttt tactgcatca atcaggttga
     1141 tgatttttgc atcaataggg tttgcaattg ttccgccact tacagaaata gaactgcctt
     1201 caaaatcaat atctattcca tcaaaaccat agaagttaat tatgctcgtc atgcttgcaa
     1261 caaaagaatc acgctcaaca gcattatcta atgaaaccgg tgctgtggca cctcccatac
     1321 tgataattac cttgcgtccc tgattctgca aattctgaac ctgcgtagca aatgctgcag
     1381 gagtaacctg ctcgggaata aattccatct gatagtcggt gcctgcctga ggcactgcaa
     1441 atgcaatgtc aataatatta taacgtgaat caacactatc aagcataatg tatggtgctg
     1501 ctgtaatatc ccaattatgc cagtaaccaa ctaatgcagg cgactgcgac aatgccacta
     1561 ccggtaagat aacgactgca aataaaagtg taagtagttt tttcatcatt atttttaatt
     1621 aaagaaattc tatcagatgc gaaaataatc aatacagatt tctattcacc ttattaaatt
     1681 cggttcaagt ttttcaatat gattcaatgc attttttatc ctgtcctctc ctattcctcg
     1741 tacagtttga tactctaatc caaagctatt caattcttgt aaatataatt caaacagttg
     1801 acttctgaaa tgcggatgtt ctcttaaagg atcatcttcc caaggtaaat caatatctgt
     1861 aagcaaatgt aatgaatagt cactcttctg aattgcatca acaatccact tttctgttcg
     1921 tccatattta aagtcgctcc aaacttttat cacgattaaa ttggtatcgc aaaacaaaaa
     1981 gtgttttgca gtttttgaca atctctcttc ttcctttaac tgcaattttg ctattaccga
     2041 caaatcgtca aaggtgtatg gacgatttaa tttatgtaaa tagtcacgtg caacttcagg
     2101 cacccaggca gtattataat gattggataa ctgttcactt aataaactct ttcctgttga
     2161 ctcaggccca acgatggcaa tcttatgaat tttcctcatg tctgatgaga tggttctgcc
     2221 atttaaaata tcctacaata gccatgataa gataaataga aaacaaaatt gcatagagcg
     2281 gcagaccttt ataaagatat attcctatat ataaaatatt cgcatctatc caaacaagcc
     2341 agttctcaat tttcttttcg gccattaaat attgtgctgc aaaacacatg cttgttgcca
     2401 atgcatccca atacggtaaa tgtgcatcag tataatgact gaaccaacca cccatgccaa
     2461 cagtaagtaa aatgattcca ccaagaagaa tgaaaatgag tttttttgaa gcgttggtaa
     2521 caggcatttc tgtgttttga ctgcctttaa tccagcgata ccaaccatag gaaagcaata
     2581 gaatgtaaac accttgttga acaacatcac tgtataatga ctgatgataa aaaatataaa
     2641 gtgatacaat tacattaatt aaactaatcg gaaagtatgc aatcttttct tttattccta
     2701 aataaattcc aaccactcca aacaaaaagc ctatccattc aatcaaagga atatttaaaa
     2761 acataatagt ttgtaagtcc acaggctaca atgcgtttaa ggtttggttt atagttgttt
     2821 ttgcttttgc aatttcaatc ataagttcgg gattatcttc aatggcattt cctaccacaa
     2881 taatatcggc acctgaatta taaatctcaa ttgctttttc ggcagtacga ataccaccac
     2941 caactatcaa aggcagacct gtattttttt taaccaattc aatcatctgt ccggatacgg
     3001 ttcttctggc tccacttccg gcttcaagat aaattacctg catgcctaaa agttgtccgg
     3061 caacagctgt agaaactgca agttcgctat gatcgtgggg aatgggtttt gtattgctta
     3121 catactgcac tgttgttaca ttaccactgt caataagaat gtatcctgta ggtatagcct
     3181 caagccctga tagttttaca gacatagcac taagcacatg ctggccaatc agcaaatcag
     3241 cattacgacc tgatattaaa gaaagatata aaattgcatc agcctgcgga tcaacttgcg
     3301 aagcgttacc aggaaaaaga atgacgggca aggatgcaaa tttttttaat ctggcaatag
     3361 tttcagaaat gcttccatgc gttaaaatac ttcctccaac aaagaaatag tcaacctgat
     3421 gctctgttgc ataagcacaa agttttttca actgatcttc attgacctta tcggggtcaa
     3481 tcaaaacagc aagctgcttc ttatggttat ggaattgctg aagtatttgt tgcttcatat
     3541 ttatttttcg atggcgaaag tagttagcaa ttggtcttga aacgaatatt gaatatcata
     3601 gttgcaaata tcattattta aaattaactg acctttaatt tcgccctgat gttgatattc
     3661 aaagggtaaa atacgcaggt tgtctttaaa atcgagcgat tttttaccat aaattttata
     3721 aataacttct ttagcgctcc aaagcatcag cgttttttgc aaaggattgt cttttaaaaa
     3781 agattcttca tcgcttcgga caaacttatg agaaatagaa gcaacaggcc tgtccattaa
     3841 ttccatgtca aaaccgcaat tgttgaaacg gtgaaacaat actcccacaa atccatgtgt
     3901 atgtgaaata gaaatgaatc cttcaggtga ggctaaaaat ggtttgccga attcattata
     3961 aaaaatatcg aagtgtttat caggcaatat ctcatgcaga atcaatcgaa ctgcatacca
     4021 ctctgcaata cgtctttggt gggtatagtg tccgggcaaa ttatactttc cataaaaacg
     4081 taacatctga gaaagcatat tagtgctttc tgtcaaatgc catattcctg taaaactatt
     4141 atcagactta gttgacttta ttagaggcat aaaatattcc tttgatagta ggcttcaaat
     4201 gtgtaaattt gcgcaaaatt ttgaaaatca tacaaattaa atcaaaaata tgtcaacagt
     4261 aacagattca aaactgaagt acaaagtaaa agacatttca caggctgagt ggggtcgtaa
     4321 agaaattaaa cttgccgagg cagagatgcc gggattaatg tcattacgtg cagaattcgg
     4381 aaagcaaaaa ccacttaaag gtgcaagaat tgcaggatgt cttcacatga ccattcaaac
     4441 ggctgtatta attgaaacat taaaggaatt aggagcagaa gttacctgga gttcatgtaa
     4501 tattttttct acacaagacc atgccgcagc agcaattgca gcagcaggaa ttcctgttta
     4561 tgcatggaaa ggtatgagca acgaagagtt tgattggtgt attgaacaaa cactttttgc
     4621 atttgaaggt ggcaagccat taaacatgat tttagatgat ggtggcgacc ttaccaacat
     4681 ggtgtttgac aaatttcctg aactgataaa agatattaaa ggatta
//
LOCUS       DAOOOI010000132         4702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3351_length_4702_cov_10.364536, whole genome shotgun sequence.
ACCESSION   DAOOOI010000132 DAOOOI010000000
VERSION     DAOOOI010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3351_length_4702_cov_10.364536"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="PKX59_10580"
     CDS             <1..1046
                     /locus_tag="PKX59_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ91344.1"
                     /translation="GFDNFSKQESTYKFDAGLSFYGYSGKRDTSKTTENSVILAGKLQ
                     RKIADRDAFADIKIDYSRTKLPFDTLVKNTIVGVYPRYTLEFPEFRLIAGVNTEWEAN
                     SAAKFHLYPHVEARYKLASDVLMIHAQVSGGMQKNTYKAMTAENPFTGDFVIPGNTNK
                     KFDVSGGLNIKLDKEILFSAGASFKRLKDAVYFENDSTGALNYTTFSTVYSDADVITI
                     NGELVFERNEKFNAHIGATYTNNKPDGLAKAWFVPAVEINLGGYILLREKIIFKTDMF
                     YRGERFASAYNGDSFQRLKPYFDANLSVEYRYSKMVSVFLNVNNIGSVQYFKWYNYPS
                     YRLQLLAGASLSF"
     gene            1274..1810
                     /locus_tag="PKX59_10585"
     CDS             1274..1810
                     /locus_tag="PKX59_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91345.1"
                     /translation="MAQRSRNTNRTHGLDVTISRLVPNFSKVPDQNFLFHESLTYSIV
                     AGQRIRAFAGINFLAPAKGGVTRYGFHTGFLAGSKNPTAGLIYGPVLFHIVNKTAYDN
                     GALRNYHNTGLAFALGVYYVPVSQFVLSTKIAPAIYHYSVRKKNSIPVIDNNSDSRSE
                     TFYMGFYNLLSLSVGYRF"
     gene            1900..3741
                     /locus_tag="PKX59_10590"
     CDS             1900..3741
                     /locus_tag="PKX59_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOZ91346.1"
                     /translation="MSKTNEVLDQVIIKFAGDSGDGMQLTGTQFTNTAALFGNDLSTF
                     PDFPAEIRAPQGTVAGVSGYQLHFGSVNIFTPGDKCDVLVAMNAAALKVNLKNLKDSG
                     TIIANSDGFDPKNLRLAKYPDGQNPIEDGSLGKYNLVVIDVTKMTRAALVDSGLGTKE
                     IDRAKNMFVLGFLYWMYNRSLDNSIKFLEEKFGKKQAILDANIKVLKAGYHYGDTTET
                     FRTRYTVQPAKMKPGVYRNIMGNQATAIGLVAASQKAGIDLFFGTYPITPASDILHEL
                     SKYKSFGIKTFQAEDEIAAVCSAIGASFGGALGVTSSSGPGIALKGEAMGLATMLEIP
                     LVVINVQRGGPSTGLPTKTEQSDLMQALYGRNGEAPMPIVSASSPADCFNAIYEACRI
                     AIEYMTPVIFLSDGYIANGAEPWQFPTADQLREFKVKYATETNGEHFLPYKRDEKLSR
                     PWAIPGTKGLEHRIGGLEKQHETGNISYDNDNHQFMVKMREAKVEKVADSIPMQTLDS
                     GPEKGKLLVVGWGSTYGAIKTACQMARKEGYEVAHAHVTYLKPFPKNFGEMLFKYDKV
                     LMPEMNMGQLSRVVRDKFMIPAIAMNKVMGIPFSATEIKEKIVELMK"
     gene            3785..>4702
                     /locus_tag="PKX59_10595"
     CDS             3785..>4702
                     /locus_tag="PKX59_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOZ91347.1"
                     /translation="MESTATTPTPLFTSKDLVTDQDIRWCPGCGDYSILKQVQTVIPE
                     LGLPREQIVFISGIGCSSRFPYYMETYGMHSIHGRATAIASGLKAARKDLSVWIVTGD
                     GDSMSIGGNHFIHLLRRNFNVNVLLFNNQIYGLTKGQYSPTSESGKVTKSTPFGSIDH
                     PFNPAALAMGADGTFVARSMDRDPVHLKEMLRQAAQHKGTSFLEIYQNCNVFNDGAFE
                     VFTEKASKKQEALFVEHGKPLVFGQNGELGIKLDGFKPVIVNHAEKGISTNDLWIHDM
                     YDRVKSGILARFFETPANEGHFPRPFGVFY"
BASE COUNT         1469 a          817 c          993 g         1423 t
ORIGIN      
        1 ttgggttcga taatttttca aagcaggagt ccacctataa gtttgatgca ggcttgtcgt
       61 tttatggtta tagcggaaaa cgtgacacca gtaagacaac agaaaacagt gtaatcttag
      121 ccggaaaact acagcgtaaa attgccgaca gagatgcatt tgcagacatt aaaattgatt
      181 attcacgtac taaacttcct tttgatacat tagttaaaaa cactattgtg ggcgtttatc
      241 cgcgttacac tttagagttt ccggagtttc gtttaattgc cggtgtcaat acagagtggg
      301 aggcaaatag tgcggctaag tttcatttgt atccgcatgt agaagccaga tataaattag
      361 ccagtgatgt tttgatgata catgcacagg tttcaggagg tatgcagaag aacacttata
      421 aggcaatgac agcggaaaat ccttttacag gtgattttgt tatacccgga aatacaaata
      481 agaagtttga tgtttcggga ggactgaata ttaagttaga caaagaaatt ctctttagcg
      541 caggagcatc tttcaagaga ttaaaagatg cagtttattt tgagaacgat tcgacagggg
      601 cattaaacta tacaaccttt tctactgttt acagtgatgc cgatgtgatt acaataaatg
      661 gcgaattggt atttgagcgc aatgaaaaat tcaatgcaca tattggagca acttacacca
      721 ataacaagcc cgatggactt gccaaggcat ggtttgttcc ggctgtagaa attaatttag
      781 gcggatatat tttactgaga gaaaaaatca tctttaaaac agatatgttt tatcgtggcg
      841 aacgatttgc ttcagcttac aatggcgact cattccagcg attaaagccc tattttgatg
      901 ccaacctttc tgttgaatac cgctattcaa aaatggtatc tgtatttctt aatgtaaaca
      961 acattggctc tgtacagtat tttaaatggt ataattatcc ttcttacagg ctgcaattgc
     1021 tggcaggtgc ttctttatcg ttttaaaatt ataagattta tttacgaggc tgcccgtttc
     1081 gggcagcctc gttgttttta ggaaatccat tgcgcattta ataaggcatc aagcttatgc
     1141 aatacactaa gttgcaaaaa ctgatgtatt attaataaaa cgatatttgc atcattgcca
     1201 atgcaatcat gagattagac aattgaaaaa tcttttcaca ttaatacttt tattctgtag
     1261 tactttaagt agtatggctc aaagaagtcg taacacgaac cgtactcatg ggttggatgt
     1321 taccatcagc aggttagttc ctaatttttc taaagtgccc gatcagaatt ttttgtttca
     1381 cgaaagtttg acttattcta ttgttgccgg acaacgtatc cgtgcattcg caggaattaa
     1441 ctttttggca cctgccaaag gtggtgtaac acgatatggc tttcatacag gatttcttgc
     1501 cggatccaag aatccaactg caggactcat ttatgggccg gtactttttc atattgtaaa
     1561 caagactgct tacgataatg gtgctctccg aaattatcat aatacgggat tggcatttgc
     1621 attgggggtt tattatgttc ctgtaagtca gtttgtttta tcaactaaga ttgcccctgc
     1681 tatctatcat tatagtgtga gaaagaagaa tagtattcct gttattgata acaattcaga
     1741 ctctcgctcc gaaacttttt acatgggttt ttataatctg ttgagcctga gtgttggata
     1801 ccgtttttaa gagaatgttg atggtttaaa aaatcatctc attacatact ttctttagca
     1861 aataaacctt tacttttgtg ttcagtaatt tttattaaaa tgagtaagac aaacgaggta
     1921 ttagaccagg taatcataaa gtttgccggt gattccggtg atggaatgca gttaacagga
     1981 acacagttta ccaatactgc tgcactgttt ggaaatgatt taagtacatt tcctgatttt
     2041 ccggccgaga ttcgtgcccc acaaggtaca gtggcaggtg tttcaggcta tcagttgcat
     2101 ttcggaagtg taaacatttt tactccggga gataaatgtg atgtgcttgt ggcaatgaat
     2161 gcagcagcat taaaagtaaa tcttaaaaat ctaaaagatt caggaactat cattgccaac
     2221 agtgatggat ttgatccaaa gaatttgcga ttggcaaaat atcctgatgg acagaatcct
     2281 attgaagatg gttcattagg taagtataat ctggtagtga ttgatgtcac aaaaatgacc
     2341 cgtgctgcat tggttgattc gggcttaggt actaaagaaa tagaccgtgc caaaaacatg
     2401 tttgtgcttg gtttcctata ttggatgtac aatcgttcgc tcgacaatag tataaaattt
     2461 ctggaagaaa aattcggaaa aaaacaagct atcctcgatg caaacattaa agtgttgaag
     2521 gcaggttatc actatggtga tacaacagaa acgttccgca ccagatatac tgtacagcct
     2581 gcaaaaatga aacccggtgt atatagaaac atcatgggaa atcaggcaac agcaattggt
     2641 ttggttgcgg catcacaaaa agcaggaata gatttgtttt ttggaaccta tccaattact
     2701 cctgcatcag acatattgca cgagctttct aagtacaaaa gttttggaat taaaactttt
     2761 caggcagaag atgaaattgc agcagtttgt tcagcaattg gtgcttcatt tggtggtgcc
     2821 ttgggtgtaa catcatcatc gggtccgggt attgctttga aaggtgaagc aatgggctta
     2881 gctactatgc tcgaaattcc attggtggtt ataaatgttc agcgtggagg cccttcaaca
     2941 ggattgccaa ctaagacaga acagagtgac ttaatgcagg cgctttatgg cagaaatgga
     3001 gaagcaccaa tgccaattgt ttcagcttct tcacccgctg attgctttaa tgcaatttat
     3061 gaggcctgcc gcattgctat tgaatatatg acgcctgtta tttttctttc tgatggttat
     3121 attgcaaatg gtgcggagcc ttggcaattt ccaactgccg atcagctaag agaatttaaa
     3181 gtgaaatatg caactgaaac aaatggagaa cacttccttc cttataaacg tgatgaaaaa
     3241 ctttctcgtc catgggcaat tcccggaaca aaagggttag aacatcgaat tggcggtctt
     3301 gaaaaacaac atgaaacagg caacatcagt tacgataacg acaaccatca gtttatggtg
     3361 aaaatgcgtg aagctaaagt ggaaaaagta gccgatagta ttccaatgca aacattagat
     3421 agcggtcctg aaaaaggaaa attactcgtt gttggttggg gaagtacgta tggtgctatt
     3481 aaaacagctt gtcagatggc ccgcaaagaa ggttatgaag tagcacatgc tcatgttact
     3541 tatctgaaac catttccaaa aaattttggt gaaatgttat tcaaatatga caaagtgctg
     3601 atgcctgaaa tgaatatggg acaactttca agagtggtga gggataagtt tatgatacct
     3661 gccatagcaa tgaataaagt tatgggaata cctttttcag caacagagat aaaagaaaag
     3721 attgttgaat taatgaaata accaaaagaa aaatttaaat cagtttttaa tagataatat
     3781 ttttatggaa tcaactgcaa caacacccac accgttattt acatcaaaag atttggtaac
     3841 cgatcaggat attcgttggt gtccgggatg tggcgattat tcaatattaa agcaggtaca
     3901 gaccgttatt cctgagttgg gtctgccacg cgaacagatt gtttttattt caggaatcgg
     3961 atgctcttca cgtttcccat attacatgga gacgtatggt atgcactcta ttcacggaag
     4021 agcaacagcc atagcaagtg gactcaaagc tgcacgcaaa gaccttagtg tttggattgt
     4081 aacaggtgat ggcgattcaa tgtctattgg tggaaaccac tttatacatc tgttgcgcag
     4141 aaattttaat gtaaacgttc tgttgtttaa caatcagata tacggactta caaaaggtca
     4201 gtattcgcca acatcagaat caggaaaagt gactaagtca acacctttcg gttcaattga
     4261 tcatcctttt aatcctgctg ctttagctat gggtgccgat ggaacttttg tggcacgaag
     4321 catggatcgc gatcctgtgc atttaaaaga aatgctccgt caggcagcac agcataaagg
     4381 aacttccttt cttgagattt atcagaactg taacgtattt aatgatggtg catttgaagt
     4441 atttacagag aaagcatcca aaaaacaaga agcactcttt gttgaacatg gcaaaccatt
     4501 ggtgttcggg cagaatggtg aattgggaat taagcttgat ggatttaaac ctgtcattgt
     4561 taatcatgca gaaaaaggta tcagcacaaa cgacctttgg attcatgaca tgtacgatag
     4621 agttaagtca ggaattttag cgcggttctt tgaaacccct gcaaacgaag gccacttccc
     4681 acgcccgttt ggagtcttct at
//
LOCUS       DAOOOI010000133         4671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3375_length_4671_cov_8.638432, whole genome shotgun sequence.
ACCESSION   DAOOOI010000133 DAOOOI010000000
VERSION     DAOOOI010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3375_length_4671_cov_8.638432"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /gene="rlmD"
                     /locus_tag="PKX59_10600"
     CDS             <1..1073
                     /gene="rlmD"
                     /locus_tag="PKX59_10600"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOZ91348.1"
                     /translation="PEMEEILPSAEVYHYRNRLDFAFSEKEWLTSDKLQNKEYQIQPA
                     LGFHITGRFDKVLNIDECLLMQEPVNRIRNFVKSFALKEGYTFFNRISQQGDLRNMLV
                     RNTTSEQWMVVMIFADADEEKRSKMLNAIKEEFPEITSLYYFINQKKNDTFFDLQPVL
                     WSGAETITENMGGLKFTIGPKTFFQTNPVQAIELYNTALSFATLLPHELVYDLYTGTG
                     TIANFMAKQCKKVLGIESVEESIEQAKLNSKSNGIKNTEFFVGDMRETLSSTFFEEHG
                     FPDVIITDPPRAGMHPDVVSRICNSGAGRVVYVSCNPSTMARDIALMKDHYTLIKAKP
                     VDMFPQTDHIECVALLTKRNLN"
     gene            1167..1970
                     /locus_tag="PKX59_10605"
     CDS             1167..1970
                     /locus_tag="PKX59_10605"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOZ91349.1"
                     /translation="MKKLMLCLLAIMTACKAGKQSVETHHAQTNFMTTQSGVKIEIKE
                     KGNGIKPSPGDRITVHYTGKLQNGTKFDSSVDRGEPFVFNVGTGQVIKGWDEAFSMLQ
                     QGDKAVLTIPPDAGYGSRAVGTIPANSTLIFEVELLRVSPKIVPTPYNVQGKDTVKLP
                     SGLQYIMVKEGTGKTAVSGATVAVHYSGYLEDGSMFDSSVERGEPISFRLGEGRVIQG
                     WEQGIALLKTGSQCRLIIPYQLGYGEQGYPPVIPAKSTLIFDVELLEVN"
     gene            1992..3263
                     /locus_tag="PKX59_10610"
     CDS             1992..3263
                     /locus_tag="PKX59_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91350.1"
                     /translation="MKKVLLITYYFDPGNAVATPRIMSWANEFHQDGIELTVVTRHFK
                     GDEWHWLDFIKETTEPIKVVKNERFSVHYLPYQSGKPALVNKNKLYSKFYHLKTFLQG
                     NFNTEINAYKAFGGYCRELLRKNPQDLILVSAPPHNLVRLAGELGKEFDLPFVVDFRD
                     IWNNLITGKNELSMRKSFSNFIEEKYIGKWLKEARLVTTVSQALVEQVKRIYNGKCIE
                     LTNGYEMKLFNQVNSTNPPSKRFIFASIGTIYPIQNISIMIEGLKIFYDKLRDKSQFE
                     IKFIGLGAIESVAQYVKSELNYLKPIITQKLSRKEVFKHYIESHVLFIPAWSNHKGIY
                     STKIFEYIGSGRKILVAPGDNDVIDNIVRKSGRGYIANSAEEFASLLSEIYDLWQSGK
                     YRAEQPAEWEQLFSREKQAELLANEILKIAK"
     gene            3260..4378
                     /locus_tag="PKX59_10615"
     CDS             3260..4378
                     /locus_tag="PKX59_10615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOZ91351.1"
                     /translation="MKEKVCFILSFHDRAVQYEWIIERALKQNLNLHFIFLNPAQWHI
                     EEYVRMRKIPCLFIKYHSKRDIPRAVALIYQYLVKEKITTVHTHLFDANLAGLTAAWL
                     ANVKKRIYTRHHSDFHHRFYPKAVRYDRYCNRLATDIVAISQNVYDILIEKEKVKPEK
                     IHLIHHGFEMELFSKVPTSEVMALREKYNTKDRFPVIGMISRYEESKGIQHVIPAFKQ
                     LLKKYPNALLVLANSHGSYSKQIKKCLEQIPIENFREIRFEYNLFALYKLFDVFVHVP
                     EYKEYEAFGQVYVEALAAGIPSVFTVSGIAAEFIKNNENALVCNYKNSDDIYNAVVKL
                     LTDDGLRNRLVENGLKVVQTKFNMDRYFSHLKKLYGYD"
     gene            4365..>4671
                     /locus_tag="PKX59_10620"
     CDS             4365..>4671
                     /locus_tag="PKX59_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91352.1"
                     /translation="MGMIEVIANEHTFHVFMFYGDETKNFVKQVSFVKAQYEKNIVLH
                     VAHINSDVEQLSSRIDELKCNFKVVFHVVQKSPFEKVSELVGILRTVEAGWICLLKPG
                     "
BASE COUNT         1575 a          671 c          974 g         1451 t
ORIGIN      
        1 atcctgaaat ggaagaaata ttgccttcgg cagaagttta tcattacaga aacagactgg
       61 attttgcgtt ctctgaaaaa gaatggttaa catctgacaa acttcagaat aaggaatatc
      121 agattcagcc tgcattagga ttccatatta ccggacgctt tgataaggtt ttaaatatag
      181 atgaatgttt gttaatgcag gaacctgtaa atcgtatcag aaattttgta aagagctttg
      241 ctttaaagga aggctatact ttttttaacc gcatcagtca acaaggtgat ttgcgaaata
      301 tgctggtaag aaacactact tcagagcaat ggatggtagt tatgatattt gcagatgccg
      361 atgaagaaaa gcgaagtaaa atgctcaatg caattaaaga agagtttcct gaaataacat
      421 cactttatta ttttattaac caaaaaaaga acgatacatt ttttgatctg caaccagttt
      481 tgtggagtgg tgcagaaact ataacggaga atatgggagg tttgaaattt acgataggcc
      541 ctaaaacatt ttttcaaacc aatcctgttc aggcaattga attgtacaat acagcattat
      601 cattcgcaac attactgccg catgagttgg tttatgattt atacaccgga acaggtacta
      661 ttgcaaattt tatggccaag caatgtaaaa aggttttagg tatagaaagt gttgaggaat
      721 ctatcgaaca ggcaaaatta aattcaaaga gcaatggaat taagaataca gaattttttg
      781 ttggcgatat gcgtgaaacg ttaagctcaa ctttttttga agagcatggt tttcctgatg
      841 tgataattac cgacccacct agagcaggaa tgcatcctga tgtggtgagc agaatttgta
      901 atagcggggc aggaagagtt gtttatgtta gttgcaaccc atcaacaatg gctcgtgata
      961 ttgcattgat gaaggatcat tacacattga taaaagcaaa gccggtagat atgtttccgc
     1021 agaccgatca cattgaatgt gtggcattac ttaccaaacg caacttgaac taattcacct
     1081 tgtagtgtga aaaactttga attgtattaa ggccggatac actatgattt ttacaatttt
     1141 gaaaaaaatg cagtatcctg ccgagcatga aaaaactaat gttatgtttg cttgctataa
     1201 tgactgcctg caaagcaggt aagcagtctg tagaaacaca tcatgctcaa acaaatttta
     1261 tgacaacaca atcaggagtt aaaattgaaa ttaaagaaaa aggaaacgga attaagccat
     1321 caccgggtga tagaatcacc gttcattata caggtaaatt gcagaatggc actaagttcg
     1381 atagttctgt tgacagagga gagccttttg tgtttaatgt tggaacaggg caagtaataa
     1441 agggatggga tgaagctttc agtatgcttc agcaaggtga caaggcagta ttgacaatac
     1501 cacctgatgc tggttatggc agtcgtgctg ttggaactat tcctgctaac tccactttga
     1561 tttttgaagt tgagttgttg cgagtgtcgc ccaaaattgt accaacacct tacaatgttc
     1621 aaggcaagga tacagttaaa ttaccctcgg gacttcagta tattatggtt aaagaaggaa
     1681 caggtaaaac tgctgtttca ggcgcaactg tagctgtgca ttacagtgga tatcttgaag
     1741 atggaagtat gtttgattct tcagtagaaa gaggtgagcc aattagtttt cgtttaggcg
     1801 aaggaagagt gattcaggga tgggagcaag gtattgcatt attaaaaaca ggctcacaat
     1861 gcagattgat tattccttat caacttggtt atggggagca gggttatcca ccggtaattc
     1921 ctgcaaaatc aacactcatt tttgatgttg aattacttga ggtcaactga tggcaattgt
     1981 taaatgctga agtgaagaaa gttctgctta taacttacta ttttgatcct ggaaatgcag
     2041 tagctacacc tcgaataatg agttgggcaa atgaattcca tcaggatggc attgaattaa
     2101 ctgtcgttac cagacatttt aagggtgatg aatggcattg gctcgatttt attaaagaga
     2161 caacagaacc aattaaggta gttaagaatg aacgtttttc tgtgcactat cttccttatc
     2221 agtcaggcaa gcctgcactt gtaaataaga ataaacttta cagtaaattt tatcatttaa
     2281 aaacatttct gcaagggaat tttaatacag aaatcaatgc ttataaagca tttggtggct
     2341 actgtaggga gttattgagg aagaaccctc aggatttaat attagtttct gcaccaccac
     2401 ataatcttgt cagattagca ggtgaattag gcaaggaatt tgatttacct tttgttgttg
     2461 atttcagaga catctggaat aatcttatta caggaaagaa tgaactgagt atgcggaagt
     2521 ccttttcgaa ttttatagaa gaaaaatata ttggcaagtg gttgaaagag gcaagacttg
     2581 tcacaacagt cagtcaggca ctagttgaac aagttaaaag aatctataat ggtaaatgta
     2641 tagaacttac caacggatat gaaatgaagt tgtttaacca ggttaacagt acaaatcctc
     2701 cttcaaaacg ttttattttt gccagcattg gaaccattta tcctattcag aacatctcaa
     2761 taatgattga aggtctgaaa atattttatg ataaactaag ggacaaaagt cagtttgaaa
     2821 taaaatttat tgggttaggt gcaatagaaa gtgttgcaca gtatgttaag tctgagctca
     2881 attatttgaa accaattatt acgcagaaat taagtagaaa ggaagtattt aagcattata
     2941 ttgaaagtca tgttcttttt attcctgctt ggtcaaatca caaaggcatt tactcaacaa
     3001 aaatttttga atatataggt tcaggtagaa aaattttagt agctcccgga gataatgatg
     3061 ttattgataa catagttcgt aaatccggaa ggggttatat cgctaatagt gcagaagaat
     3121 ttgcatcatt attaagtgaa atatatgatt tgtggcagag tggaaaatat agagctgagc
     3181 aacctgctga gtgggagcaa ttattttcaa gagagaaaca agcagaactt cttgcaaatg
     3241 agattttgaa aattgcaaaa tgaaagaaaa agtttgtttt atattgtctt tccatgatcg
     3301 tgctgtacaa tatgaatgga ttattgaacg tgcgttaaaa caaaatctta acttgcattt
     3361 tatttttcta aatcctgctc agtggcacat tgaagaatat gtgaggatga gaaaaattcc
     3421 atgtttgttt attaagtatc attcaaagag ggatattcct cgtgctgtag cgttgattta
     3481 tcaatacctg gtaaaagaaa aaattacaac agttcataca catttgtttg atgctaatct
     3541 ggcaggactc acagcagcat ggttagccaa tgttaagaaa agaatttata cgaggcatca
     3601 ttccgatttt catcatagat tttatccaaa agcagttcgt tatgatcgtt attgtaacag
     3661 gttggctacg gatattgttg ccataagtca gaatgtgtat gatatattga ttgagaaaga
     3721 gaaagtgaag cctgagaaaa tacatttaat ccatcatgga tttgaaatgg aattattttc
     3781 aaaagttccc acttcggaag taatggcact tcgagagaaa tataatacta aagataggtt
     3841 tcctgtaatt ggaatgatta gtcgttatga agaaagtaaa ggaatacagc atgtcattcc
     3901 tgcctttaaa caattattga aaaagtatcc taatgcactt cttgtattgg caaacagtca
     3961 tggttcatat tcaaagcaga ttaaaaaatg tctggagcag ataccaatag aaaattttcg
     4021 ggaaatcaga tttgagtaca atttgtttgc attatataaa ttatttgatg tttttgttca
     4081 cgtacctgag tataaggagt acgaagcctt tggtcaggta tatgtagaag cacttgcagc
     4141 cggaattcct tctgttttta ctgtttccgg aatagcagca gagttcatca agaataatga
     4201 aaacgcactt gtttgtaact ataagaattc agacgatatt tataatgcag tcgttaaatt
     4261 gttgactgat gacggtctta gaaataggtt agttgaaaat ggattgaaag tagtgcaaac
     4321 gaaattcaat atggataggt attttagcca tcttaagaaa ctttatgggt atgattgagg
     4381 tgatagctaa tgaacatact tttcatgttt tcatgtttta tggagatgag acgaagaatt
     4441 ttgttaagca agtttccttt gtaaaggcgc aatatgaaaa aaatattgtt cttcatgttg
     4501 ctcatattaa tagcgatgtt gaacagctaa gtagcaggat tgatgaactg aagtgtaatt
     4561 ttaaagttgt ttttcatgta gtgcaaaaat cgccttttga gaaagtcagt gaattggttg
     4621 gcatcttaag aactgttgaa gcaggatgga tttgtcttct taagccgggt g
//
LOCUS       DAOOOI010000134         4630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3413_length_4630_cov_9.960874, whole genome shotgun sequence.
ACCESSION   DAOOOI010000134 DAOOOI010000000
VERSION     DAOOOI010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3413_length_4630_cov_9.960874"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2134)
                     /gene="priA"
                     /locus_tag="PKX59_10625"
     CDS             complement(<1..2134)
                     /gene="priA"
                     /locus_tag="PKX59_10625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOZ91353.1"
                     /translation="MTEKSSSGFYIKVVVPLPLPNLFTYSVADALISQIEIGKRVLVQ
                     FGSQKIYAAIVLETSVAKPEDYEVKPILSVIDEIPVVTEKQVALWQWVHSYYLCFWGD
                     VMQAALPAELRLSSETKIVLNTEKETGSEYLTDDEFLVLEALHSVKELSLKEISRILD
                     RKHVFPVVKTLLQKKLVLSVENIGEDFKMPMANFIRRTAQTNDQDFLKKQFDHFEKKA
                     PRQLDVLMQLMQLEKNYNEGKVPQKVLLHKSGATAAALKQLVRKGLIEILIERATGSK
                     SFFNKDFYELSEAQSTALKEITKSFTEKPVTLLHGVTSSGKTELYIRLIDECLKNGKQ
                     ALYLLPEIALTTQIITRLEKHFGNKLMVYHSRFSDNERASVYTRMIENGFTNSEPFVV
                     IGARSSVFLPFHKLGLVIVDEEHESSYKQFDPSPRYHARDTAIVLAKLFEANVLLGSA
                     TPSIETYNNAITGKYGLVELFARHADIAPPQIQLVNIKELTFKRQMKSHFSPMLVNAI
                     KEELDKGKQIILFQNRRGFAPILQCHQCNWVPHCQHCDVALTYYKKADLLRCHYCGFT
                     QQPPSKCKSCGETDIRQYGFGTEKIEDELQIFFPEHNIARLDLDSTRGKHAYSEIISR
                     FETGETDILVGTQMVTKGLDFDNVSLVGILNADNLINFPDFRAYERSFQMLMQVSGRA
                     GRKKEQGKVIIQSYNPGHSILEFVVKNDF"
     gene            complement(2131..>2526)
                     /locus_tag="PKX59_10630"
     CDS             complement(2131..>2526)
                     /locus_tag="PKX59_10630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOZ91354.1"
                     /translation="RSSFDEVPEETKLFFRDSYDHCIQILDIVESYKEVTASLMDVYL
                     SSVSHKLNQVMKVLTIISTIFIPLTLIVGINGMNFQLANPETGERLPMNMPELYSPYG
                     YVGVIAVMVLIVVIQLYIFHRKGWIRFRE"
     assembly_gap    2527..2554
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2555..3267)
                     /locus_tag="PKX59_10635"
     CDS             complement(<2555..3267)
                     /locus_tag="PKX59_10635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOZ91355.1"
                     /translation="MAKIRRLSVKLSRKKTTHPGAQPGSFSIPSDALTPKALCFIYDK
                     DSFQEIDVTQFDQLKTAYQNLGEKTLWLHVAGFGDKTFYENLTALFQLHRLELEDVFN
                     VYQRPKVDEYEGHLFFVSRLLSMTDETLNNDQLSLILGKNFVITIQEKYQDFFDPIRL
                     RLRNGKGNMRTYRPGYLAYALMDAVIDHYYPALEKIGLKLDDLEDELLSNPSQDSMNR
                     ILQIKRELIVFRRAIWPER"
     gene            3329..4057
                     /locus_tag="PKX59_10640"
     CDS             3329..4057
                     /locus_tag="PKX59_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOZ91356.1"
                     /translation="MQKWFQSWFDSPYYHILYKDRDNQEAEYFMDNLLEQIHLPVKSS
                     VLDIACGKGRHSLFLADKGYHVTGIDLSEQSIAYCKQFERQSLEFYVHDMREIFRTNC
                     YDVALNLFTSFGYFNSDHDNRLALRTASLALKPGGYFILDFFNPDFIKNTSQQHIKQV
                     DGIDFSINKHSDGNTIEKTIQFSDSGKSFKFTESVQIISKHTFLKHFERVGLVTRHIF
                     GDYSLRPFEEDSSERMIFITQKPQ"
     gene            4054..>4630
                     /locus_tag="PKX59_10645"
     CDS             4054..>4630
                     /locus_tag="PKX59_10645"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOZ91357.1"
                     /translation="MTAFIILFLLITGGGLLYFLLNKSNDSLLKLSLAFSGAYLFGIT
                     ITHLVPEVFSQGDKSIGIYVMTGFLFQIILEYFSKGIEHGHIHLHEHKQHAVFPVSMM
                     ISLCIHAFFEGVPMADNQQRQSLMMGIAMHHIPVAFALMSMLMQSGVSKTVSVFSLVV
                     FAAMSPVGAFFGIYLGDTLMTEWFSKIMAIVI"
BASE COUNT         1357 a          822 c          825 g         1598 t
ORIGIN      
        1 tgaagtcgtt tttaactaca aattcgagta tagaatgccc tgggttataa gattgaatga
       61 ttacttttcc ttgctctttt tttcttccgg cacgaccact tacctgcatc aacatttgaa
      121 aactccgctc atacgctctg aaatctggaa agtttataag gttgtccgca tttaagatac
      181 caaccaaact aacattatcg aagtctaatc cctttgtaac catttgtgtt cctactaaaa
      241 tatctgtctc accggtttca aagcgactga tgatttcact gtaggcatgc ttacctcttg
      301 tgctgtcaag gtctaaacgt gcaatgttat gttccggaaa aaatatctgc aactcatctt
      361 caattttctc agtgccaaat ccgtactgac ggatatctgt ttctccacaa gacttacatt
      421 tagatggcgg ttgttgtgtg aaaccacaat aatgacaacg taacagatcg gcttttttat
      481 aataggttaa agctacatca caatgctgac aatggggaac ccaattgcat tgatgacatt
      541 gcagaatagg agcaaagcca cgtctgtttt gaaatagaat gatttgtttt cctttatcta
      601 attcttcttt tatggcatta accaacattg gtgaaaaatg tgatttcatc tgccgtttaa
      661 aagtgagttc tttaatattc accaattgaa tttgtggtgg tgcaatatct gcatgtcttg
      721 caaacagttc taccaagcca tattttccgg tgatggcatt gttgtaagtt tcaattgatg
      781 gggttgcgct gcctaaaagt acattggcct caaaaagttt agctaataca attgctgtgt
      841 cgcgtgcatg atagcgtggt gaaggatcaa attgtttgta ggatgattca tgctcttcat
      901 ccacaattac aagacctaat ttatggaagg gtagaaatac agatgaacgt gctccaatta
      961 ctacaaatgg ctctgaattt gtaaagccat tttctatcat tcttgtatag acagatgccc
     1021 tttcattatc actgaatcgt gaatggtaaa ccatcagttt attgccgaaa tgtttttcaa
     1081 gtcttgtaat gatttgcgtt gtcaaagcaa tttcaggaag aaggtaaagt gcttgcttgc
     1141 catttttaag acattcgtca ataagtctta tgtacaactc ggttttgccc gatgaggtta
     1201 ctccatgcag cagtgttaca ggtttttctg taaaagattt agtgatttct tttaatgcag
     1261 ttgattgtgc ttcgctaagt tcatagaaat ctttattgaa aaatgacttt gagcctgttg
     1321 ctctttcaat aagaatttca atcaaccctt ttcttactaa ttgttttaat gctgctgcag
     1381 ttgctcctga tttatggaga agcacttttt gtggaacctt gccttcatta taattttttt
     1441 ctaattgcat taattgcatc aaaacatcta actgtcttgg tgcttttttt tcgaaatggt
     1501 caaattgttt tttcagaaaa tcctgatcat tagtttgtgc tgtcctcctg ataaagttag
     1561 ccattggcat tttaaaatct tcaccaatgt tttctacgga taatacaagt tttttctgaa
     1621 gtaatgtttt aacaacagga aatacatgtt tgcggtcaag aatacgtgat atttctttca
     1681 ggcttaattc ttttacagaa tgtaaagctt ccaataccaa aaactcatca tcagtcagat
     1741 attcacttcc ggtttctttt tcggtattaa gtacaatttt tgtttcactg ctcaaacgta
     1801 attctgccgg tagtgctgct tgcatgacat cgccccaaaa acacaaataa taactgtgaa
     1861 cccattgcca cagtgcaacc tgcttctctg taacaactgg tatttcatca attactgaaa
     1921 ggataggctt gacttcgtaa tcttcaggtt ttgccacaga agtttctaaa actatagctg
     1981 cataaatttt ctgacttcca aattgtacca atactctttt cccaatttct atctgagaaa
     2041 ttaatgcatc tgctactgaa taggtaaata aattcggtag tggtagtggc actactactt
     2101 taatataaaa tccactgctg cttttctctg tcattctctg aaacgtatcc aacccttacg
     2161 atgaaaaatg tacaattgaa taacaactat aagcaccatc acagcaatga cacccacata
     2221 accatagggg gaatacaatt caggcatatt cattggcaga cgttctcctg tttctggatt
     2281 tgcaagttga aagttcatgc cattgatgcc aactattaat gttaatggga taaaaattgt
     2341 tgaaatgata gtgagtactt tcatcacctg attaagttta tgactaacgc ttgagagata
     2401 tacatccatt aaacttgcag taacctcttt atagctctca acaatatcaa gtatctgaat
     2461 gcaatggtcg taagaatctc tgaaaaataa ttttgtttct tcaggcactt cgtcaaatga
     2521 tgaacgnnnn nnnnnnnnnn nnnnnnnnnn nnnntctctc tcaggccaaa tagctcttct
     2581 gaatactatc agttcccttt ttatttgaag tattctgttc attgaatcct gagaaggatt
     2641 gcttagtaat tcatcttcca aatcatctaa cttaagtcca attttttcaa gtgcgggata
     2701 gtaatggtca ataactgcat ccattaatgc ataggccaag taaccgggac gatatgttcg
     2761 catattgcct tttccgttcc tcaatcttag tcgaattgga tcaaaaaagt cctgatattt
     2821 ttcctgaatg gttatgacaa aattttttcc tagaattaaa cttaactgat cgttattaag
     2881 tgtttcatca gtcatgctaa gcagtctgct cacaaaaaat aagtgacctt catattcatc
     2941 aacctttggt cgttgatata cattaaagac atcttccagt tccaatctat gtaattgaaa
     3001 aagtgcagtc aggttttcat aaaaggtttt atctccaaat ccggcaacat gcagccaaag
     3061 tgttttttca cctaaatttt gataagcagt ttttaattga tcgaattgag taacatcaat
     3121 ctcctgaaaa gaatctttgt catagataaa acaaagtgcc ttaggtgtta aagcatctga
     3181 tggtatcgaa aaacttccgg gttgtgcacc tggatgtgta gtttttttac ggctgagttt
     3241 tacacttagc cttctgatct ttgccattat taaataaatg aggttgtcaa aaatagcgat
     3301 aaatttgccg gctgaagaaa ggtattttat gcaaaagtgg tttcagtcgt ggttcgattc
     3361 gccttactat catattctgt acaaggatag ggataatcag gaagcagaat attttatgga
     3421 taatcttttg gaacaaatcc atttaccggt aaagtcaagt gttttagata ttgcatgtgg
     3481 taaaggccgc cacagtttat tccttgccga caaaggttat catgttaccg gtattgattt
     3541 gtcggagcaa agtattgcat attgtaaaca gtttgaaaga caaagtcttg aattctatgt
     3601 acatgatatg cgtgaaattt tcagaaccaa ttgttatgat gttgcactga atctatttac
     3661 cagttttgga tatttcaatt cagatcacga caatcgttta gctttaagaa cagcttcatt
     3721 ggcattgaaa cctgggggat attttattct tgattttttt aatcctgact ttatcaaaaa
     3781 cacttcacaa caacacataa aacaagtaga tggtattgat ttcagcataa ataagcactc
     3841 tgatgggaat accatagaga aaacgattca gtttagtgat tcaggaaaga gttttaagtt
     3901 tactgagtct gtgcagatta tttcaaaaca cacatttctt aaacactttg aacgggtagg
     3961 cttggtaaca agacatatct ttggcgatta ttctttgaga ccttttgaag aagatagcag
     4021 tgaacgaatg atttttataa ctcaaaaacc gcaatgacgg catttattat tttattcctg
     4081 ctgattacag gtggaggact gctttatttc ttattgaata aaagcaacga ttcattgctg
     4141 aaactctctc tggcattttc aggtgcatat ttgtttggaa ttaccattac acatttagtt
     4201 cctgaagttt ttagtcaggg cgataaatcc attggcattt atgtaatgac aggtttttta
     4261 tttcagatta ttcttgaata tttttcaaag gggattgagc atgggcatat tcatttacat
     4321 gagcataaac aacatgcagt gtttccggta tcaatgatga ttagtttatg tattcatgca
     4381 ttttttgaag gtgtaccaat ggctgataat caacagcgcc agtcgctcat gatgggaatt
     4441 gctatgcacc atattccggt tgcatttgca ctgatgagta tgcttatgca gtcaggtgtt
     4501 tctaagacag tttctgtttt ttcattagtc gtatttgccg caatgtcacc tgtaggtgca
     4561 ttctttggta tttatcttgg ggatacgctg atgactgagt ggtttagtaa aattatggca
     4621 attgtaattg
//
LOCUS       DAOOOI010000135         4589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3448_length_4589_cov_12.358403, whole genome shotgun sequence.
ACCESSION   DAOOOI010000135 DAOOOI010000000
VERSION     DAOOOI010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3448_length_4589_cov_12.358403"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PKX59_10650"
     CDS             complement(<1..256)
                     /locus_tag="PKX59_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91358.1"
                     /translation="MLLFFPLMSLAQQTNIYTDVEWRYKSALDLLRKEKFSAAREEFT
                     KALEEPISEAARQDAMYYIGLCAAELYHPDAEFLLTDFISK"
     gene            522..1373
                     /locus_tag="PKX59_10655"
     CDS             522..1373
                     /locus_tag="PKX59_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91359.1"
                     /translation="MNKSVIYGIVALAIVMSISSCKKDKSDADYDYSMATDMSLAENT
                     MEEVTKMADQAGDYGQLMTYRNSIPATVTVIGCATVTIENVTASIKKMTLDFGVTDVT
                     CIDGKRRRGKIFVQFEGNYRDANHEHTISFENYFVNDYKIEGTRKITNTGKNNQNQSV
                     FSIEADISISNPNGKTMTWQSQRTRTWITGENTTFNTDSINGVKDDIYSVTGSGSGKS
                     FNGTSFTANITEPLTVSLQCRFITKGKVELTPGEQAIRYIDYGDGTCDAIAAITINGV
                     TYYREIP"
     gene            complement(1433..2659)
                     /locus_tag="PKX59_10660"
     CDS             complement(1433..2659)
                     /locus_tag="PKX59_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOZ91360.1"
                     /translation="MTTVSKSGGMFSSESVLLPRESMLEVQKTKREVLIGIPKEISFQ
                     EHRIALTPDSVAVLTSNGHQVMIETGAGDSANFTDTDYSEAGARIVYSAREVYTADII
                     IKIAAPTNDELDLMHDKQTLFSVLQVNMQSKDFFLKMAKKKITAIGYEYMKDDSSLMP
                     VIQAMSEIVGRTSILIASELLSNTTGGKGELLGGIAGVPPSEVVILGAGTVGENAART
                     AMGLGADVKIFDDNMYKLRRVIRNLGEHVFTSTLTQSTMLKALRTCDVAIGALHARTG
                     RTPIVVTEEMVSEMKFGSVIIDVSIDQGGCFETSKPTNHTQPTFREYGVVHYCVPNIA
                     SRVAKTASHALSNIFTPILLNVGEHGGLESLLWKDASFRSGVYLFRGTVTNKYVSDLY
                     RLPFRDLNLLMASFTF"
     gene            complement(2656..3075)
                     /gene="tsaE"
                     /locus_tag="PKX59_10665"
     CDS             complement(2656..3075)
                     /gene="tsaE"
                     /locus_tag="PKX59_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOZ91361.1"
                     /translation="MNEHVFVCNSQDELSNVAKQILAVVGDSKIVLFKGEMGAGKTTL
                     IRAICQQLKVTDNVSSPTFSIVNEYHASDEVIYHFDFYRINNLREVYDMGYEEYFFSG
                     HRCFIEWPEKIEGIINFDYTLVQVEAKDEIRTIIVTI"
     gene            complement(3081..>4589)
                     /locus_tag="PKX59_10670"
     CDS             complement(3081..>4589)
                     /locus_tag="PKX59_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HOZ91362.1"
                     /translation="KPHILFLNDKGYETVTANSGTDALEIFKKEPFDIVFLDENMPGL
                     SGIETMVRIKSINSSIPVVLITKSEEESIMEDAIGSQIADYLIKPVNPNQILLSLKKN
                     LDNKRLISEKTNTQYQQEFRQIGMMLGDRLGHAEWAELYKKLIYWELQLDKVRDESMF
                     EILIQQKEEANRLFSKFVESNYLSWIKNPESNAPLMSQHVLKRKVIPVAETSDVPVFF
                     ILIDNLRFDQWKVIEPVISELFRVTEEDSYFSILPTATQYARNSLFAGLMPADIEKMH
                     PKWWVNDDDEGGKNLHEEDLLKDQISRLRKNIKSSYTKITNLDAGKELVDNIPNLFGN
                     KLNVIVYNFVDMLSHARTDMQVIRELADDEPAYRSITLSWIEHSPLLEALKRIAQKNV
                     KLIITTDHGTIRVKEPSKIIGDRNTNTNLRYKQGKNLNYEKRDVFEIKNPADALLPKL
                     NVSSTYVFAKQDKFFAYPNNFNYYVNHYRNTFQHGGISLEEMIVPFISLVNK"
BASE COUNT         1423 a          951 c          783 g         1432 t
ORIGIN      
        1 acttactgat gaaatctgtc agcaaaaatt ctgcatcagg gtgatataat tctgctgcac
       61 ataaaccaat ataatacatt gcatcctgac gtgccgcttc agaaattggc tcttccaatg
      121 cttttgtaaa ctcttcgcgt gctgcactga atttttcttt tcgtaacaaa tccagtgctg
      181 atttataacg ccactccaca tcggtgtaaa tgttggtttg ctgtgccaac gacatcagtg
      241 gaaaaaacaa taacattaaa tatatacttc gcctgaccat tctaaccatg tttaattgct
      301 gatttaagat aaaatgcaaa attattgatg tgtttagggc gctaatgatt ttacgcctgt
      361 ttatttgtct ttttatgaac agaaaaatga ggataactaa gttgttttca gtcataaaat
      421 ttctgattgc tgatgaataa tcaccccaaa atcatgataa caaaaaatca ttgaaaccat
      481 ttaccggaat taacgtatag gcatttaaaa aattatccaa aatgaacaaa tcagttattt
      541 acggaatagt tgcacttgca atcgtaatga gtatttcatc ttgcaaaaaa gataaaagcg
      601 atgctgacta tgattactct atggcaacag atatgtcttt agcagaaaat acaatggagg
      661 aagtcactaa aatggctgat caggccggag attacggtca gttgatgaca tacagaaata
      721 gcattcctgc aacagttaca gttataggtt gcgcaacagt gaccattgaa aatgtaactg
      781 catctatcaa aaaaatgact cttgactttg gagtaactga tgttacgtgc atagatggca
      841 aaagaagacg cggaaaaatt ttcgttcagt ttgaaggaaa ttatcgagat gccaatcatg
      901 agcacaccat aagcttcgaa aattattttg taaacgacta taaaattgaa ggcacaagaa
      961 aaataaccaa taccggaaaa aacaatcaga atcaatctgt attttctata gaagcagata
     1021 tcagtatcag taatcctaat ggcaaaacaa tgacatggca aagtcagcgt acacgcacat
     1081 ggattaccgg agaaaacaca actttcaata ccgacagtat caatggtgtt aaggatgata
     1141 tctactcagt taccggaagt ggcagcggaa aatcttttaa cggtacctct ttcactgcaa
     1201 atattactga gcctttgact gtttccttgc aatgtcgttt tatcaccaaa ggtaaagtag
     1261 aactgactcc cggtgagcag gctatccgat acattgatta cggtgatggc acttgcgatg
     1321 ctattgcagc aataactatc aacggagtaa catactatag agaaatacct taatgatttt
     1381 tttgaaacaa aatattcaac ccgccattgt gcgggttttt tgttgcctga atttaaaatg
     1441 taaaacttgc cataagcaaa tttaaatcac gaaaaggcaa acgatacaaa tcactgacgt
     1501 atttattggt caccgtaccc ctgaacagat aaacccccga acgaaaagaa gcatctttcc
     1561 acaataaact ttcaagtccg ccatgttcac caacattcaa cagaatggga gtaaaaatgt
     1621 tgctcaatgc atgagaagct gttttggcta cacggcttgc aatattagga acacaataat
     1681 gcaccacacc atactctctg aaagtaggtt gtgtgtgatt ggtgggcttt gatgtttcga
     1741 aacatccacc ctgatcaata ctgacatcaa taattacaga accaaatttc atttcgctaa
     1801 ccatctcttc tgtaactaca attggtgtac gtcctgtgcg tgcatgtaag gcaccaatgg
     1861 caacatcgca agtgcgcaac gccttcagca tggtggactg tgttaatgta ctggtaaaaa
     1921 catgctcgcc aagatttctg attacacgtc tgagtttgta catattgtca tcaaaaattt
     1981 tgacatcagc accaagtccc atagctgtac gtgcagcatt ttctcctact gtaccggcac
     2041 caagtataac aacttccgaa ggtggcaccc ctgcaatgcc tccaagcaac tctccttttc
     2101 cgccagtagt gttactcaat aattctgatg caataagtat tgaagtgcga cccacaattt
     2161 cactcatggc ttgtattacc ggcatcagcg aagaatcatc cttcatatac tcataaccga
     2221 ttgctgtaat tttctttttg gccattttca gaaagaaatc tttgctctgc atattaacct
     2281 gcaatacaga aaacaaagtc tgtttatcat gcattaaatc aagctcatca ttggtaggtg
     2341 ctgcgatttt aattatgata tctgccgtat aaacctctct ggcactgtaa acaattcggg
     2401 cacctgcttc gctgtaatct gtatcagtaa agtttgcgga atcaccggca ccggtttcaa
     2461 tcattacctg atgcccatta cttgtcagca cagccaccga atcgggagtt aatgcaatac
     2521 gatgctcctg aaatgaaatt tcttttggaa taccaatcag cacttctctt tttgtctttt
     2581 gcacttcaag catgctttcg cgtggaagca aaaccgattc cgaagaaaac attcctccgc
     2641 tctttgacac agttgtcata tcgtaacaat tatggtgcga atttcgtctt tcgcctccac
     2701 ttgcacaagt gtatagtcaa aatttataat accttcaatc ttttctggcc attcaataaa
     2761 acaacggtgt ccactaaaaa aatattcttc atagcccata tcataaactt ctctcaggtt
     2821 attgattctg taaaagtcga aatgataaat cacttcatcg gaagcatgat actcgtttac
     2881 aattgaaaaa gtagggctgc tgacattgtc agtcactttt aattgctggc aaattgccct
     2941 tatcaatgtt gtcttacctg cccccatctc gcccttaaac aaaactattt tactatctcc
     3001 aacaacagca agaatctgct ttgcaacatt gcttaattca tcttgcgaat tacaaacaaa
     3061 aacatgctca ttcattacct tcatttgtta accaatgaaa taaaaggcac aatcatctct
     3121 tcaagcgaaa tacccccatg ctgaaatgtg tttctgtaat ggtttacata ataattgaag
     3181 ttgttaggat atgcaaaaaa tttatcttgt ttggcaaata cataggtgct actcacattt
     3241 aattttggca acaatgcatc agcaggattt tttatttcaa agacatcacg tttttcataa
     3301 ttcagatttt ttccttgctt ataacgcaga ttggtattgg tatttctgtc gccaataatt
     3361 tttgatggct ctttaactct gatagtgcca tgatcggttg tgattatgag ttttacattt
     3421 ttctgtgcaa tacgtttcaa tgcctccagc aatggcgaat gttcaatcca ggaaagtgtg
     3481 atggaacgat atgcaggctc gtcatctgca agttcgcgaa taacctgcat gtctgtacgt
     3541 gcatgagaaa gcatatccac aaaattgtaa actataacat tgagtttgtt gccaaacaaa
     3601 ttcggaatat tatcaaccaa ttctttccct gcatcaaggt tagtgatttt agtataggat
     3661 gattttatat tttttcttaa acggctgatc tgatctttca gtaaatcttc ctcatgtaaa
     3721 ttttttccac cttcatcgtc atcatttacc caccattttg gatgcatttt ttcgatgtcg
     3781 gcaggcatga gtcctgcaaa cagactgtta cgtgcatact gtgttgcagt tggcagaata
     3841 ctgaaataag aatcttcttc cgttactctg aaaagttcac tgatgacagg ttcaataact
     3901 ttccactgat cgaaacgcag gttatcaatc agaataaaaa acacaggcac atccgatgtt
     3961 tctgcaacag gaataacttt tctttttagc acatgttgcg acatgagcgg tgcattactt
     4021 tcaggatttt ttatccaact cagataatta ctctcaacaa attttgaaaa cagtctgttg
     4081 gcttcttctt tctgttgaat cagaatttcg aacatacttt catcacgcac tttatcgagt
     4141 tgcaactccc agtaaataag ttttttatac aactctgccc actctgcatg cccaagtctg
     4201 tcgccaagca tcatacctat ctgcctgaat tcctgctgat actgtgtgtt tgttttttca
     4261 ctgatcaatc tcttgttatc aagattcttt ttcagcgaca ataaaatctg attcggattt
     4321 acaggtttta tcagatagtc tgcaatctga gaaccaatgg catcttccat tatagactct
     4381 tcttcactct tggtgatcag gacaacagga atactactat tgatactctt aatgcgcacc
     4441 atcgtttcaa tgccactgag tccgggcata ttctcatcta agaaaacgat atcgaagggt
     4501 tcttttttga aaatttctaa tgcatctgtt ccgctgtttg ctgtaacagt ttcataaccc
     4561 ttatcattaa gaaataaaat gtgtggctt
//
LOCUS       DAOOOI010000136         4489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3536_length_4489_cov_8.837393, whole genome shotgun sequence.
ACCESSION   DAOOOI010000136 DAOOOI010000000
VERSION     DAOOOI010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3536_length_4489_cov_8.837393"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1608
                     /locus_tag="PKX59_10675"
     CDS             <1..1608
                     /locus_tag="PKX59_10675"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOZ91363.1"
                     /translation="LDALQQLASFHRNKFNLKTIGITGSNGKTIIKEWLYQLLQPDFS
                     IVRSPKSFNSQTGVPLSVWQINNEHNLGIFEAGISIPGEMQKLEKIIQPEIGIFSNVG
                     EAHSENFSSLDVKIQEKLLLFQHCKTLIYCKDYSGIHNAVIQSGYLENGLKCFSWSRR
                     IKSDLQIGRIEKQNNSSTIQGIFQQKFIRIEIPFTDDASIENAIHCWAMLLFLEIEQE
                     TIAERMMLLSPVAMRLEMKQGINNCSIINDSYNSDLGSLQVALDFLNRFQQQNNTGAI
                     NSGLRKTVILSDILQSGRNEDQLYGEVAEMIHSKKVDRLIGIGPAISRHTELFKTEKV
                     FFSTTEKFIEQYSPGSFINEVILLKGARVYGFEQISRLLQQKGHETVLEINLTSMIHN
                     YNFYKAKLKPETKIMCMVKAFAYGSGSYEVANTLQFHRVDYLAVAYADEGIELRKNGI
                     TVPIMVMNPQEQSFDKMIEYHLEPEIYSFRIMSKFSESVKRIRTYNQSPFPIHLKMDT
                     GMHRLGFEEDDLNELVVRLKKTVNTLR"
     gene            1626..2237
                     /gene="alr"
                     /locus_tag="PKX59_10680"
     CDS             1626..2237
                     /gene="alr"
                     /locus_tag="PKX59_10680"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOZ91364.1"
                     /translation="MAGTDEAALDFFTQEQFQRFKDMSDAIKKHLQHNVLEHVLNSSG
                     ISRFPEMQLSMVRLGIGLHGIGTPSEQRQLLNVASLKSTISQIRSVKKGESVGYNRKA
                     ILDSDKIIATIGIGYADGINRKMGNGKVSFLVNGKLAPTVGNICMDMCMLDITNCQAR
                     EGDEVIIFGNGLPVAELAAKTETIPYEILSVISQRVKRIYFQE"
     gene            complement(2238..3509)
                     /locus_tag="PKX59_10685"
     CDS             complement(2238..3509)
                     /locus_tag="PKX59_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOZ91365.1"
                     /translation="MLRRVLIFLLIVFLIDLYVFQGIKVLIKDLTPSMSRLISGIYWF
                     FTVFTFSVFIAGLITDWHTWPKAVRVYLFAAVMIILITKLFVVLFLLIDDLTRLVRWG
                     FSGLYQRFYDSPQIVNPEEKIVVSRSQFLVRMAFVISSIPFISLLYGMGDGKYNYQVK
                     RRKIKFPKLPSTFEGFRIVQISDIHTGSFFDTKHIEKAVSMINTLKGDVIFFTGDLVN
                     DRSDEAVPFIDTLSKLNAKHGVFSILGNHDYGDYVQWPSREAKVKNLEQLKNIHKEMG
                     WKLMLDEHAFISEGNDKLGLIGVQNWSTHMRFPKYGSLPKAVQGMEFAPVNILLTHDP
                     SHWHGEVTENYPQIDLTLAGHTHGFQFGVEIPALKIKWSPVQYVYKEWADLYDDVNGQ
                     HLYVNRGLGFLGYPGRVGILPEITVIELSKG"
     gene            3585..>4489
                     /locus_tag="PKX59_10690"
     CDS             3585..>4489
                     /locus_tag="PKX59_10690"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HOZ91366.1"
                     /translation="MKDTAQPTNKLIHEDSLYLQQHAHNPVHWLPWSVDAFEKAKTEN
                     KLLIISIGYSSCHWCHVMEKESFENTTIAEVMNENFICIKVDREERPDIDAVYMTAVQ
                     LMKGQGGWPLNCIALPDGRAIYGGTYFNPQQWLQVLEQLSMMYEQDNSKFLEYASSLQ
                     EGMLQLEKIETTNTKPDFDILKNSISKWSSLFDKIYGGLQRAPKFAMPVNLNFLMQYG
                     HVCNDVEVQNHVRLTLNKMYNGGIFDQLDGGFARYSTDSRWEIPHFEKMLYDNAQLIS
                     VYAQAFRAYNNISFKNAAEETINFL"
BASE COUNT         1574 a          820 c          789 g         1306 t
ORIGIN      
        1 cttgacgcac tacaacaact tgcctcgttt catcggaata aatttaatct gaaaacaata
       61 ggaataactg gaagcaatgg aaaaacaatt atcaaggaat ggctttatca attattgcaa
      121 ccagatttta gtattgtccg cagcccaaaa agttttaatt ctcaaacggg cgtaccatta
      181 tctgtttggc aaataaacaa tgagcataac ctcggaattt ttgaagcagg catttctata
      241 cctggtgaaa tgcaaaagtt ggaaaaaatt attcagcctg aaattggaat tttcagtaat
      301 gtcggtgaag cacacagcga aaacttttct tcacttgatg ttaaaattca ggaaaaatta
      361 ttgttatttc aacattgcaa aaccctgata tactgcaaag actattcagg aattcacaat
      421 gctgtaatac aatctggata tcttgaaaac ggactaaaat gttttagctg gtcgcgaaga
      481 ataaaatccg atttacagat tggcagaata gaaaagcaaa ataacagcag cacaattcag
      541 ggtatttttc aacagaagtt tataaggatt gaaatacctt ttactgatga tgcttctatt
      601 gaaaatgcta ttcattgctg ggcaatgttg ctctttttgg aaattgaaca agaaacgatt
      661 gctgaaagga tgatgttact gagtcctgtt gccatgcgat tagagatgaa acagggtatc
      721 aataattgtt caataattaa tgacagctat aattctgatt taggttcgtt gcaggttgcc
      781 ttagattttt taaatcgctt tcagcagcaa aacaatacag gagcaattaa ttcaggctta
      841 cgcaaaaccg ttatcctttc agacattctt cagagtgggc gaaatgaaga tcaactttat
      901 ggtgaagtgg cggaaatgat tcactctaaa aaagttgaca gacttattgg aattggccct
      961 gcaatatcac gtcatacaga gttatttaaa acagaaaaag tttttttttc tacaacagaa
     1021 aaattcattg aacagtattc tccaggctca tttattaatg aagttatatt attaaaaggc
     1081 gcaagggttt atggatttga acagatttca agattgttac agcaaaaagg tcatgaaaca
     1141 gttttggaaa taaatctgac ttcaatgatt cataattata acttctacaa agcaaagctc
     1201 aaaccggaaa caaaaataat gtgtatggtt aaagcttttg catacggtag tggcagttac
     1261 gaagttgcca atacactgca atttcacaga gtggactatc tggctgttgc ctatgctgat
     1321 gaaggtattg aactcagaaa aaatggtatt actgttccaa ttatggttat gaatccgcag
     1381 gaacagtctt tcgataaaat gattgaatat catttggagc ctgagatata tagtttcaga
     1441 atcatgagca agtttagtga atctgttaaa cgaatccgga cttacaatca aagcccattt
     1501 cctattcatc taaaaatgga tacaggcatg cacaggcttg gctttgaaga agacgatttg
     1561 aatgaactcg ttgtacgttt aaaaaaaaca gtaaacacct taaggtagaa tctattttta
     1621 cacatctggc aggaactgat gaagcagcac tcgacttttt tacacaagaa cagtttcagc
     1681 gattcaaaga catgtctgat gctataaaaa aacaccttca gcacaatgtg ttggagcatg
     1741 ttctgaattc atcaggcatc agtcgctttc ctgaaatgca actgagcatg gtgcgtcttg
     1801 gcattggcct tcatggcata ggcacacctt ctgagcaacg tcaattatta aatgttgctt
     1861 cattgaagtc aaccatttca caaataagaa gtgtaaaaaa aggagaaagt gttggttaca
     1921 acagaaaggc aatattggat agcgataaaa taatcgcaac cataggcatt ggttatgccg
     1981 atggcataaa ccggaaaatg ggaaatggaa aggttagctt tttagtgaat ggcaaacttg
     2041 ccccaacagt tggcaacatc tgcatggaca tgtgtatgct tgacattaca aactgtcagg
     2101 cacgcgaagg tgatgaagta attatttttg gcaacggttt gcccgttgcc gaacttgctg
     2161 caaagacaga aaccattcct tacgaaattt tatcagtgat ttcgcaacgt gtcaaacgaa
     2221 tatattttca ggaataatta acctttcgat aattctatta cagtaatttc cggaaggata
     2281 cctaccctac ccggataacc aagaaaacca agtccgcgat ttacatacaa atgctgacca
     2341 tttacatcat catacaaatc agcccactct ttataaacgt actgaacagg gctccattta
     2401 atttttaatg caggaatctc aacaccaaac tgaaatccat gagtatgccc tgctaaagtt
     2461 aaatcaatct gtggataatt ttcagttact tctccatgcc aatgcgaagg atcgtgcgta
     2521 agtaaaatat taacgggagc aaattccatt ccctgaactg cttttggcag gctaccatac
     2581 tttggaaaac gcatgtgtgt actccaattc tgtactccaa ttaatcccaa cttatcattg
     2641 ccttcagaaa taaatgcatg ttcatcaagc atcagtttcc aacccatttc cttatggata
     2701 tttttcagtt gctccaaatt tttaacttta gcttctctgc ttggccattg cacatagtcg
     2761 ccataatcgt gattacctaa aatagaaaaa acaccatgtt ttgcattgag ctttgacaat
     2821 gtatcaataa aaggcactgc ctcatcagag cggtcattca ccaagtcacc ggtaaaaaaa
     2881 atcacatccc cttttaatgt gttaatcatt gaaacagctt tttcaatatg ttttgtatcg
     2941 aaaaagctgc cggtatgaat atcagaaatc tgcacaattc taaagccttc aaatgttgaa
     3001 ggtaattttg gaaacttaat tttcctccgt ttgacctgat aattatactt accatctccc
     3061 atgccataca agagagaaat aaatggaatt gatgaaatta caaatgccat tcgcactaaa
     3121 aactgcgatc tcgatacaac aatcttctct tcaggattta caatttgtgg agaatcataa
     3181 aatcgctgat ataaacctga aaatccccat ctgactaatc tggtaagatc gtcaatcaac
     3241 agaaacaaaa caacaaagag ttttgttatt aaaattatca taacagcagc aaagagataa
     3301 actctcactg cctttggcca tgtatgccaa tctgtaatca gtccggctat aaatactgaa
     3361 aatgtaaaca ctgtaaaaaa ccaataaata cctgaaatca atcttgacat tgagggagtc
     3421 aaatccttaa tcaatacctt tataccctga aaaacataaa ggtctatcag aaaaacaatt
     3481 aataaaaaaa tcaatactct tcgcaacata aaaacgcaca aatgtaagtg aaactattca
     3541 tcaatacatt tctatcaatt aaacttttct atatataatt cacaatgaaa gatacagcac
     3601 aaccaacaaa caaactcata catgaagaca gtctgtatct gcagcagcat gcccataatc
     3661 ctgtacattg gctaccttgg tccgttgatg catttgaaaa agcaaagact gaaaataagt
     3721 tacttataat cagcattggt tattcaagct gtcattggtg tcatgtgatg gagaaagaaa
     3781 gttttgaaaa tacaactatt gctgaagtga tgaatgaaaa ctttatatgc attaaagttg
     3841 atcgtgaaga aagacctgat atagatgcag tttatatgac agccgttcaa ttgatgaaag
     3901 gacaaggtgg ctggccacta aactgtattg ccttacctga cggcagggca atctatggcg
     3961 gcacctactt caatccacaa caatggttgc aggtgcttga acaattgtca atgatgtatg
     4021 aacaagataa ttcaaaattt ctggaatatg cttcttcatt gcaggaaggc atgttacaat
     4081 tagaaaaaat tgaaacaaca aatactaaac ctgattttga tattttaaaa aattcaatat
     4141 caaaatggtc ttcactcttt gacaaaatat atggcggcct tcaacgagca ccaaaatttg
     4201 caatgcctgt taacctgaat ttcctaatgc aatatggtca tgtttgtaat gatgttgaag
     4261 tgcaaaatca cgtcagatta acattgaaca aaatgtacaa cggaggtatt ttcgatcagc
     4321 ttgatggagg atttgcacgt tattctactg acagtcgttg ggaaattccg cactttgaaa
     4381 aaatgctata tgacaatgcc cagctaataa gtgtctatgc acaagcattc agagcatata
     4441 ataacatttc ttttaaaaat gctgctgaag aaacaataaa cttcctgaa
//
LOCUS       DAOOOI010000137         4276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3735_length_4276_cov_7.294243, whole genome shotgun sequence.
ACCESSION   DAOOOI010000137 DAOOOI010000000
VERSION     DAOOOI010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3735_length_4276_cov_7.294243"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PKX59_10695"
     CDS             complement(<1..687)
                     /locus_tag="PKX59_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91367.1"
                     /translation="MKIFSLFLFSLLICSNIYGQQLSIENKPALNEESKSVDSWVVHV
                     DQDMNFCFSAYSEFMRKNFDYRINRRSKIVHSVDKKLFTEISNLRIDQRAIFTQESKG
                     TAIAFQFSPGYDIHFGNTMYKEEFDRARAMVVNYVHFHYKMFYDDQIKNLRSRIKGLE
                     KNISNNQSKIEKNTKTVNLNAEDISNNAVGADKLREKNEKLEKDNVTLTTEIDKFRSQ
                     ITQLETEIDNA"
     gene            complement(689..1957)
                     /locus_tag="PKX59_10700"
     CDS             complement(689..1957)
                     /locus_tag="PKX59_10700"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOZ91368.1"
                     /translation="MKIDVLLGLQWGDEGKGKLIDVLAPDYDVIARFQGGPNAGHTLE
                     FDGIKHVLHTIPSGIFRENKLNIIGNGVVIDPLIFKKENDSLLKLGIDIKKNLWVSRR
                     AHLILPTHRLLDAASETMKGKTKIGSTLKGIGPTYMDKTGRNGLRVGDIERHDFKDAL
                     NYLLNKHAELLSFYNHPTVSKEDLEMWLESISTLREMTLVDSEYEINSLINSGQRILA
                     EGAQGSLLDIDFGSYPFVTSSNTITAGACIGLGVPPSAIGKVFGVFKAYCTRVGSGPF
                     PSELNDATGDELRKKGNEFGSTTGRPRRCGWLDLPALKYTAMVNGVTSLMMMKSDVLS
                     GFENLEVCNSYKVNGQSSVRMPFNVVNDIPEPEYITFKGWQNDISKANSFESLPSELH
                     NYIQAIEKYIGIPLDVVSVGPDRTQTIIRK"
     gene            complement(1972..2313)
                     /locus_tag="PKX59_10705"
     CDS             complement(1972..2313)
                     /locus_tag="PKX59_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOZ91369.1"
                     /translation="MNFSFTTSEKGNFCIIKLSGNLMEKGQANELMDEINTCIIRDMK
                     TFVLDLADLRFMSSSGLTVLLNILTKSRKAGGDTIICNINSQLKSVFTITRLTEVFTI
                     TENQEAAIAAI"
     gene            complement(2310..2792)
                     /locus_tag="PKX59_10710"
     CDS             complement(2310..2792)
                     /locus_tag="PKX59_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOZ91370.1"
                     /translation="MAEAEFLKTEKEIFTEYMISNGLRKTPERYAVLEEAYRTNGHFN
                     VDMLFELIQKRKLRVSRATVYNTIEHLLKCGLITKHQFGETKFIYEKSFAYKQHDHLI
                     CGDCEKVFEFCDPRLYQIQSTVEKYLDFKITQHALNFYGQCNKLKQTGACEHFKVKKQ
                     "
     assembly_gap    3119..3152
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3153..>4015
                     /locus_tag="PKX59_10715"
     CDS             <3153..>4015
                     /locus_tag="PKX59_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91371.1"
                     /translation="ALYYQSVASGNWNATTTWQSSVAAGGPWAPAGATPTSADQTVTI
                     MAGTVVRITTGITIDEVVVSPGATLIANSSANLIINNGAAVDLIINGTFVDSLGGAAN
                     INFNAGATWQISAGATIVKCTVSSCNNWQSSYQGGIASIPATANWIFRKVGLSNPSVS
                     TTNGGTQAYYPNFIYENYTSTPFITGSYANSHFNGSASYTIIKGSMDIGGSGTNYVNF
                     TSNNINPTIGVQVRGDVIIRPGNTYNNIGTGTEVWGDVYMDGTWKYYNGNGRRLVFSG
                     NANSTVWGSGH"
     assembly_gap    4016..4035
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4036..4260
                     /locus_tag="PKX59_10720"
     CDS             <4036..4260
                     /locus_tag="PKX59_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91372.1"
                     /translation="HELTMAKILPASITLNRSLMVDSLVNFNSGKIYSSATNPLIFNV
                     GAKANTVSNNNSFVEGPVRKLGSTAFVFPV"
BASE COUNT         1259 a          827 c          716 g         1420 t
ORIGIN      
        1 agcattgtca atttctgttt ctaactgtgt tatctgtgat ctgaatttat ctatttctgt
       61 tgttagtgtc acattatctt tttctagctt ttcattcttc tcacgcaact tatctgctcc
      121 tacagcatta tttgaaatat cttcagcatt aagattaaca gttttagtat tcttttcaat
      181 ttttgactga ttgttgctga tatttttttc taatcctttg attctcgatc tcaggttttt
      241 tatctgatca tcataaaaca ttttgtaatg aaaatgcacg tagtttacaa ccatggctct
      301 tgcacggtca aactcctcct tatacatggt gttgccaaaa tgtatatcat agccggggct
      361 gaattgaaaa gctattgcag taccttttga ttcttgtgta aatattgctc gctgatctat
      421 ccgcagattt gaaatttcgg taaataattt tttatctact gaatgaacaa ttttacttct
      481 gcggttaata cgataatcaa aattcttacg cataaattcg ctgtaagcag agaaacaaaa
      541 attcatatcc tgatctacat gtactaccca cgaatcaaca ctctttgact cttcgtttaa
      601 agcaggctta ttttctattg acagttgctg tccgtaaatg ttggagcaaa taagtaagct
      661 gaataaaaat aatgaaaata ttttcatttt attttctgat tattgtttgc gttctatcgg
      721 ggccaactga tacaacatct aatggaatac cgatgtattt ttcaattgcc tgaatgtagt
      781 tgtgcaattc agatggtaaa ctttcaaaac tatttgcctt actgatatca ttctgccatc
      841 ctttaaaggt aatgtattcc ggctccggaa tatcattcac tacgttaaac ggcattcgta
      901 cagaagattg cccattaact ttataagaat tacaaacctc taagttttca aaaccactaa
      961 gtacatcaga tttcatcatc atcagacttg taacgccatt aaccattgca gtatatttca
     1021 aagcaggtaa atcgagccat ccgcatcgtc ttgggcgacc ggtagtacta ccaaattcat
     1081 tgcctttttt acgcaactca tctcctgttg catcatttaa ctcagatggg aaaggaccac
     1141 tgcctacacg tgtgcaatat gctttgaaca caccaaacac tttacctatt gccgaaggag
     1201 gaacaccaag cccgatacat gcacctgctg tgattgtatt gcttgacgta acaaatgggt
     1261 aagatccaaa atcaatatca agcaatgagc cttgtgcccc ttctgccaaa atacgctggc
     1321 cggaattaat caaactgttt atttcatatt cactgtcaac caatgtcatt tcgcgaagtg
     1381 ttgaaatact ctcaagccac atttcgaggt cttccttgct gactgttgga tggttgtaga
     1441 atgaaagcag ttctgcatgt ttgtttaata aataatttaa tgcatcttta aaatcatgcc
     1501 tttcaatgtc gccaacgcgc agaccgttac gtccggtttt atccatatag gttggaccta
     1561 ttccttttag tgtagaacca atctttgttt tacccttcat ggtttctgat gcagcatcaa
     1621 gcaaacgatg tgttggcaga atgagatgtg ctcttcgcga tacccaaaga tttttcttta
     1681 tgtctattcc taacttaagc agtgaatcat tttctttttt gaaaatcagc ggatctatta
     1741 ccacaccatt accaatgatg ttaagtttat tttcacgaaa aattcctgac ggaatggtat
     1801 gcaacacatg ttttattcca tcaaactcca aggtatgtcc tgcatttggc ccaccctgaa
     1861 aacgtgcaat cacatcatag tcgggagcta aaacatctat cagttttcct ttgccctcat
     1921 cgccccactg aagtccaagt aatacgtcta ttttcataat tattttttta ttcagatggc
     1981 agcaatagca gcttcctgat tctctgttat ggtaaaaacc tctgtcaatc gtgtaatagt
     2041 aaatacagat tttaactgac tatttatatt gcaaataatg gtgtcgccac cggccttgcg
     2101 cgatttggtc aggatgttta ataaaacagt aagtccgctg ctgctcataa aacgcaaatc
     2161 tgctaaatcg agcacaaacg ttttcatatc tctgataata caggtattga tttcgtccat
     2221 cagttcatta gcctgtcctt tctccatgag gttgcccgaa agtttaataa tacagaaatt
     2281 tcccttttct gatgtggtaa aactaaaatt cattgttttt ttactttaaa atgttcgcat
     2341 gcccctgttt gtttgagttt attacactgt ccataaaaat tcagggcatg ttgtgttatt
     2401 ttgaaatcta aatatttttc tactgttgac tgaatctgat aaagcctcgg gtcgcaaaat
     2461 tcaaatactt tttcgcaatc gccacaaatc aaatgatcgt gttgcttata ggcaaaagac
     2521 ttttcgtaaa taaatttggt ctcgccaaac tgatgtttgg ttattaagcc acatttcaat
     2581 aaatgttcaa tagtattata aacggtggca cggcttactc ttaacttgcg tttttgaatc
     2641 agttcaaaga gcatatccac attaaaatga ccattagtgc gataagcttc ttcaagtact
     2701 gcataacgtt caggtgtttt gcgtagccca ttgcttatca tatactctgt aaaaatttct
     2761 ttctctgtct ttaaaaattc cgcttctgcc acgttaaata aatgccattt taattgctac
     2821 aaaagtaata aatcaaactg ttatagaatt tccaattaca actgagatac aaatagttgt
     2881 tcttcactta ctttttcttc caaattgttg ataaattgct ttccgcttat ctctgatttt
     2941 taaatgaaaa aaacctcaat agacctatat cacaacaata acaaaattca ttttttgtcg
     3001 taaaaaaata tttaatgcca acggcaacaa aaatgcaatg ttttagaatt atatcaagta
     3061 cttttgccaa ttgaaccata caacactgtt ttgacattga aatcgttttt caggctatnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngctctata ctatcaatca gttgcatcgg
     3181 gcaattggaa tgctacaact acatggcaaa gtagtgtagc agcaggtggt ccttgggctc
     3241 cggcaggcgc aacccccaca tcagcagatc agactgtaac aattatggcc ggtactgtag
     3301 tacgcattac tacaggtatt acgattgatg aagtagttgt cagcccgggt gcaacattaa
     3361 ttgcaaattc ttctgctaat ttaatcatta acaatggcgc agctgttgac ctaatcatta
     3421 acggaacttt tgttgactca ttaggaggtg ctgcaaatat taattttaat gccggtgcta
     3481 cctggcaaat aagtgccggt gcaacaattg ttaaatgtac tgtatcgtca tgcaataact
     3541 ggcagtcaag ttaccaaggc ggcattgctt ctattcctgc aacagccaac tggattttca
     3601 gaaaagtagg tttgtctaat ccttctgtca gtacaacaaa tggcggaact caggcatatt
     3661 atcccaattt tatttatgaa aattatactt ccacgccatt tataaccggg tcttatgcca
     3721 attcgcattt taatggttct gcaagctata ctatcattaa aggtagtatg gatataggtg
     3781 gttcaggaac aaactatgtc aactttacaa gtaataacat caacccaacc attggtgtac
     3841 aggtaagagg tgatgttata atccgtcctg gaaatacata caacaatatt ggtacaggta
     3901 ctgaagtttg gggtgatgtt tatatggatg gcacatggaa atattataat ggtaatgggc
     3961 gcagattggt tttttcagga aatgccaaca gcacagtttg ggggagcggc catttnnnnn
     4021 nnnnnnnnnn nnnnncacga attgacaatg gctaaaattc ttcctgcaag cattacatta
     4081 aaccgttctt tgatggtaga ttcattggta aactttaatt caggaaaaat ttattcaagt
     4141 gctaccaacc cattaatttt taacgtagga gcaaaagcaa atactgtatc taacaacaac
     4201 agttttgtag aaggtcctgt aagaaaatta ggcagtactg cttttgtttt tccggtttga
     4261 atggcaaatg atgtgc
//
LOCUS       DAOOOI010000138         4203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3794_length_4203_cov_12.399229, whole genome shotgun sequence.
ACCESSION   DAOOOI010000138 DAOOOI010000000
VERSION     DAOOOI010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3794_length_4203_cov_12.399229"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="PKX59_10725"
     CDS             <1..1056
                     /locus_tag="PKX59_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ91373.1"
                     /translation="FSSDGKTLYFVRGSNSRTNDQDIFMSEVSEKGWSTPVKLNNNIN
                     TAGKEEAVFIHPDNQTLYFVSDGWPGMGGSDIFMSKRQADGQWGKAVNLGYPINTFAN
                     EAGLIVSPSGDKAYFSSNREGGLGGLDIYSFDLYDKIKPDPVSYAIGYVYDDETKKPA
                     DAKFEIIDIESGKTVVESYSDKNTGEFMVTLPYGKNYMLNVSKRKYLFYSDHFEMDKP
                     SDIKNAKQLIVRLKPVKDGSTMALNNIFFDTNSAELKPESKTELNKLVSFIKSNAIAK
                     LEIGGHTDNVGDDKANMLLSDKRAKSVCDYLITSGIEASRLISRGYGETKPVSDNTTE
                     EGKAKNRRTEITILSVH"
     gene            1154..2722
                     /gene="dnaB"
                     /locus_tag="PKX59_10730"
     CDS             1154..2722
                     /gene="dnaB"
                     /locus_tag="PKX59_10730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOZ91374.1"
                     /translation="MEENVEAGRSAKKKVAPKSLTNQQIALGLGKIPPQAVDMEEAVL
                     GALMLEKNALTAVIDILHTEAFYKEAHQKIFKVIHDLFHKAEPIDILTVTHALKKSGD
                     LELVGGAFYITQLTNRIASAANIEYHARIILQKFIQRELIRISSETIKSAYEDTSDVL
                     KMLDDAEKNLFSIAEGNIRRNYENMNSLITKAIREIEIASKQTQGITGVESGFTDLDR
                     ITAGWQKSDLIIVAARPGMGKTAFTLSLAANAALQFNRPVAFFSLEMSSVQLVNRLLS
                     SQAEITSEAIRKGQLAEHQWQQLNSKIAPLSDAPIFIDDTPALSVFELRAKCRRLVAE
                     KKVQLIIIDYMQLMVAGTDNRNGNREQEISTISRSLKAIAKELEVPIICLSQLSREVE
                     KRGTASKRPQLSDLRESGAIEQDADMVMFIYRPEYYGMDVDDEGHSTKNTAEIIIAKN
                     RNGSLNTVKLTFIGQYTKFKDRELENQTPQDKAIYGYTDESNKVRIPNPNVEAENRYT
                     RGSKMNDMPDEDPF"
     gene            complement(2729..3631)
                     /gene="rfbD"
                     /locus_tag="PKX59_10735"
     CDS             complement(2729..3631)
                     /gene="rfbD"
                     /locus_tag="PKX59_10735"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOZ91375.1"
                     /translation="MLRILLTGSNGLLGQKIVYAAIKHSDIQLLATSKGENRLHTKAG
                     YDYVSMDITNADEVTAIVENFKPHCIINTAAMTNVDACEKDQENCWKMNVEAVRTMTE
                     LCQLHDIHFIHLSTDFVFDGEKGNYSEEDAPNPLSHYARSKFESEKVVQQAEIDSAIL
                     RTIIIYGVVDDKQRSNVVIWTINSLRQQKNINVISDQMRSPTLAEDLADACLQAALKR
                     ATGIYHVSGSETMSILEMVNRTADFFQLDKKYIHPITTAQLNQPAKRPLLTGFNIDKA
                     KRELNFQPHTLQQGLEIVKSQLEN"
     gene            complement(3644..>4203)
                     /locus_tag="PKX59_10740"
     CDS             complement(3644..>4203)
                     /locus_tag="PKX59_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91376.1"
                     /translation="KKSFIIEKGFTQGVDVVLKYEYRRMYAWLVYSLGFSTRNTGNYE
                     YAPHFDRRHNINIIGSYKLGKKLDWEVDARWNFGSGFPVTLNQGFYPYLNFQDGLATD
                     YTQNNGEMGVLYGPLNESRLPTYHRLDISIKKIFYLARHSELEVVASVVNVYNRKNVF
                     YVDRIRHNVVNQLPIMPSLGASLTF"
BASE COUNT         1382 a          749 c          852 g         1220 t
ORIGIN      
        1 ttttcgagtg atggcaagac cttatacttt gttcgtggca gcaacagccg caccaacgat
       61 caggacattt ttatgagtga ggtgagtgaa aaaggatgga gtacaccggt gaaactaaac
      121 aacaacatca acacagcagg aaaagaagaa gccgtattta ttcacccgga taatcaaacg
      181 ttatattttg tttctgacgg atggccgggc atgggaggct cagatatctt tatgagtaaa
      241 cgccaggctg atggtcagtg gggcaaagca gtaaacttag gatatccaat aaatacattt
      301 gccaatgaag caggattgat tgtaagtcct tcgggcgata aagcctattt cagcagcaac
      361 agagaaggtg gattaggagg tttggatatt tacagtttcg acttgtatga taaaattaaa
      421 ccagaccctg tgagttatgc aataggatat gtgtatgatg atgaaacaaa aaaacctgcc
      481 gatgcaaaat ttgaaatcat tgatattgaa tcgggcaaga cagttgtaga atcttattct
      541 gacaaaaaca caggagagtt tatggtgacc ttgccttatg gaaaaaatta tatgcttaat
      601 gtgtcgaagc gaaaatatct tttctactcc gatcattttg aaatggataa accatcggat
      661 attaaaaatg caaagcagtt gatagtaaga cttaaacccg tgaaagatgg cagcaccatg
      721 gctttgaata atattttctt tgacaccaat tctgcagaac taaaacctga atcaaaaaca
      781 gagttaaata aactggtgag ttttattaaa agtaatgcca ttgcaaaact tgaaattgga
      841 gggcatactg ataatgtagg tgatgacaaa gcaaacatgc tgttgtctga caagcgagca
      901 aaatcggttt gtgattatct tattacatca ggcattgaag catcgcgatt gatttccagg
      961 ggttatggag aaacaaaacc tgtgtcagat aatacaactg aggaaggaaa ggcaaaaaac
     1021 agaagaacag aaatcactat attatcagtt cattaatttt tttgcataca aaatttttct
     1081 gttaaatacg tgttgaaaat tatttaaaaa tattttcgga gaagggggcg tggcaattga
     1141 ctaaatttgc tgcatggaag aaaatgtgga agcaggcagg agtgcaaaaa agaaagtagc
     1201 tcccaagtcg ttaacaaatc aacagattgc attaggatta ggcaaaattc caccacaggc
     1261 tgtggatatg gaagaagcag tattaggcgc attaatgctt gaaaaaaatg cactcactgc
     1321 tgttatagat attttacata cagaagcttt ctataaagaa gcacatcaga aaatatttaa
     1381 agtcatacat gatctttttc ataaagctga gccaattgat attttaacag ttacacatgc
     1441 tttgaaaaaa tcaggcgatt tagaattagt aggtggtgca ttttatatta cgcagttgac
     1501 caacagaatt gcttctgcgg ctaacattga atatcatgcg cggattatcc ttcaaaaatt
     1561 tattcagcgt gagttgattc gtatttcgag tgaaactata aagagtgctt atgaagacac
     1621 atcagatgtt ttgaagatgc tggacgatgc agagaaaaat ttattttcaa ttgcagaagg
     1681 aaacattcgc agaaattacg aaaacatgaa ttctcttatt accaaagcca ttagggaaat
     1741 agaaattgca tcaaaacaaa cacaaggcat tacaggagta gaaagtggct ttactgactt
     1801 agatcgtata acagcaggat ggcaaaaatc tgatttgata attgttgctg caagaccggg
     1861 tatgggaaaa acagccttta ccctatcgtt agcagccaat gccgcattac aattcaacag
     1921 accggttgcg tttttctcat tggagatgtc aagtgttcag ttggtaaacc gtttgctttc
     1981 ttcacaggca gaaataactt cagaagccat cagaaaaggg caacttgcag aacatcaatg
     2041 gcaacagcta aacagcaaaa tcgctccgct ttctgatgca cctattttta ttgatgatac
     2101 cccggcatta tcggtttttg aacttcgtgc caagtgcaga agacttgttg cagaaaagaa
     2161 agtacaactc atcattattg attatatgca attgatggtg gcaggaacag acaatcgtaa
     2221 tggaaaccgt gagcaggaaa tcagtaccat ttcgcgttca ttaaaagcta tagctaaaga
     2281 attagaagtt ccgataattt gtttgtcgca gttgagtcgt gaagtagaaa agcgcggaac
     2341 agcttctaag cgcccgcaat tgagcgacct tcgtgagtca ggagctattg agcaggatgc
     2401 tgatatggta atgtttattt accgcccgga atattatggt atggatgtag atgatgaagg
     2461 ccattcaaca aaaaatactg cagaaattat tattgccaaa aacagaaatg gttctttaaa
     2521 taccgttaaa ctcaccttta ttggtcaata tactaagttt aaagaccgtg agctagaaaa
     2581 tcagacacca caagataaag caatatatgg ctatactgat gaaagcaata aggttagaat
     2641 acctaatccg aatgttgaag cagagaatcg ttacacacgt ggttcaaaaa tgaacgacat
     2701 gcctgacgaa gatccttttt agtctgactt aattttctaa ttgactcttt acaatttcca
     2761 aaccttgctg caaggtgtga ggctgaaagt ttaattcacg ttttgcttta tcaatattaa
     2821 aacctgttag caaaggacgt tttgcaggtt gattgagttg agcagtagtg atgggatgaa
     2881 tatatttttt atccagctga aaaaaatctg ctgtacggtt taccatttca agaatgctca
     2941 tagtttcact gccggataca tgatatattc ctgttgcgcg ttttaatgca gcctgcaggc
     3001 aggcatcagc aagatcttcg gcaagtgtag gtgagcgcat ctgatcgctg atgacattga
     3061 tatttttttg ctgacgcagt gagtttattg tccagataac aacattactt ctttgcttgt
     3121 catccactac tccataaatg atgattgtac gtaaaatagc agaatctatt tctgcttgct
     3181 gcacaacttt ttccgattca aatttactgc gggcataatg actcaacgga ttgggggcat
     3241 cttcttcgct gtagttgccc ttttcaccat caaaaacaaa atcggtactc agatgaataa
     3301 aatgtatgtc atgaagttga cacagttcag tcattgtcct gactgcttct acattcatct
     3361 tccagcaatt ttcctgatct ttttcgcagg catcaacatt ggtcattgca gcagtgttga
     3421 tgatacaatg cggcttaaaa ttctcaacaa tggcagttac ttcatcagca ttggtaatat
     3481 ccattgaaac atagtcatag cctgcttttg tatgcaaacg gttttcacct ttagaagttg
     3541 caagcagttg tatgtcgcta tgctttatag ctgcataaac aattttctgc cccagcaatc
     3601 cattacttcc tgtcaataaa atacgcagca tgacattgca aaattaaaac gtgagcgatg
     3661 ctcccaatga tggcattatt ggcaattgat ttaccacatt gtgtctgatg cgatcaacat
     3721 aaaacacatt tttacgatta taaacattga caacacttgc caccacttcc agctcactgt
     3781 gtcgggcaag atagaaaatt ttcttgattg aaatatcgag acgatgatag gtcggtaatc
     3841 gcgactcatt taatggtccg tagagcacac ccatttcgcc attgttctga gtatagtcag
     3901 tagcaagtcc atcctgaaaa ttgagataag gataaaatcc ctgatttaaa gttacaggaa
     3961 aacctgatcc gaaattccag cgggcatcaa cttcccagtc gagtttttta cctaacttat
     4021 aagagccgat gatattgata ttatgtcgtc tgtcgaaatg tggagcatat tcgtagttgc
     4081 ctgtattgcg tgtgctgaat cccaaagaat aaacgagcca tgcatacatt cttctgtatt
     4141 catatttcaa aacaacgtca acaccttgtg taaatccttt ttcgatgata aaacttttct
     4201 taa
//
LOCUS       DAOOOI010000139         4179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3820_length_4179_cov_8.474054, whole genome shotgun sequence.
ACCESSION   DAOOOI010000139 DAOOOI010000000
VERSION     DAOOOI010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3820_length_4179_cov_8.474054"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PKX59_10745"
     CDS             <1..671
                     /locus_tag="PKX59_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOZ91377.1"
                     /translation="RELLEYHFNRVTETCIPEEEAFRRLQKHIHQIWKDADNKGERYF
                     PHEYMYKMLLSGVLKQYYEIDPKNSWMLAAAQKNIPIVVPGWEDSTMGNIFASYVIKN
                     EMKATTMKSGIEYMVWLSDWYRQNSGGKGVGFFQIGGGIAGDFPICVVPMMYQDLEWH
                     DVPFWSYFCQISDSTTSYGSYSGAVPNEKITWGKLDIDTPKFIVESDASIVAPLMFAW
                     ILEW"
     gene            681..1223
                     /locus_tag="PKX59_10750"
     CDS             681..1223
                     /locus_tag="PKX59_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOZ91378.1"
                     /translation="MNAAVKYTLAGIAAVILGFVSLGIIHPEIEYESRVQISAPVTKA
                     FDIFIDTSMMKHWMPGFVSFKPLEGKMLTKGSKQLLVLNLNNEQIEITETVEDYSYPK
                     FYKFHAQNTLLNSTNTIIFEGDSIHCSLTVKTKASGNNFMLRSMFVFTEPIFKQQQDS
                     TYTLLKRTIESHLDNHNINQ"
     gene            1271..2938
                     /locus_tag="PKX59_10755"
     CDS             1271..2938
                     /locus_tag="PKX59_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOZ91379.1"
                     /translation="MNSRILNFLLLLLISTSVNAQSDTELKSNLKQHISILASDKFEG
                     REPGTKGERLSYEYITQQFKNIGLQSLGSNGYLQSFEFSAGTKNGVKNKLTIAGKNLK
                     SDSEYYVLPYSALQATAEGKSIKIGSGLLSKDLGRNDYEGLTVLKGRIAVMDYATPEG
                     NNPHSKWAEASDLRKRIEIAVSKGISGLIIYNSNDSIKNPEKNSEIKITPFAIPVVFI
                     KSNDATLLKDNQQVTIATDIEKIMHSGHNVIGYIDNRAENTIVIGAHYDHLGYGGHES
                     LYRGKPEIHNGADDNASGTSTLIELARKLNSASLKKYNYLFIAFSGEEKGLLGSNYFV
                     KHPTIDLKKVNYMLNMDMVGRLKPEEHILLINGAGTSPSWDSLLNNIKVDSLRIKTTA
                     SGVGPSDHTSFYLEGIPVLHFFSGTHSDYHKPSDDEPLINYNGMVSIFHFMYDLIEQT
                     NNLPKLTFTKTNDSSNEDAPRFKVTLGVVPDYAFEGSGMRIDGVTEGKPAAIAGFLKG
                     DVVIQMGEEKVADMMSYMKALSKFKKGDTVKVKVQRGKDVVEKEVTF"
     gene            2955..3440
                     /locus_tag="PKX59_10760"
     CDS             2955..3440
                     /locus_tag="PKX59_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91380.1"
                     /translation="MTQRTIHFEEKQYLGLNHYSLLRSMVLALFCFVAYYVTENRENN
                     AELLFFGGVAFMVISLLSMFIKHLHTRVYDSCIELDGLWTMRKVKIDLSTIVDVQKIP
                     YSNYIMNTPVYNLHRNGSIYFYTSGRSAIKITDKDGMNYIIGTQKQEEFLRILNDLIK
                     K"
     gene            complement(3486..3764)
                     /locus_tag="PKX59_10765"
     CDS             complement(3486..3764)
                     /locus_tag="PKX59_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91381.1"
                     /translation="MKKTVLTLAVVAATYCANAQTEKKAEDQAQPVKSEQATTPQKEV
                     KKDAPATIQAAPANHESAPADKEVKKDAPQKDSHKATEMEKPKDKPKE"
     gene            complement(3822..4127)
                     /locus_tag="PKX59_10770"
     CDS             complement(3822..4127)
                     /locus_tag="PKX59_10770"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOZ91382.1"
                     /translation="MKERTSQIGIQKSLGAKNYFILMQFLFEAIVLCLIGGLIGLSII
                     YFLAMFVSHAFDYPLSLTGHNIMLGLFISALIGLVSGFIPAYSASQLDPVEAIRANG"
BASE COUNT         1433 a          694 c          794 g         1258 t
ORIGIN      
        1 aaagagaatt attagaatat cattttaacc gtgttactga gacttgtatt cccgaagaag
       61 aagctttcag aagattacaa aaacatattc atcagatatg gaaagatgcc gacaataaag
      121 gcgagcgtta ttttcctcat gagtacatgt ataaaatgct tttgagtggt gtgttgaaac
      181 aatactatga gattgatcct aaaaactcat ggatgcttgc agcagcacaa aaaaatattc
      241 caattgtagt tcccggctgg gaagacagca ctatgggaaa tatttttgca tcatacgtca
      301 ttaaaaatga aatgaaagca acaaccatga aaagcggtat tgaatatatg gtttggctta
      361 gcgactggta tcgtcagaat tcaggcggta agggcgttgg attttttcag attggtggtg
      421 gtatagcagg tgactttccg atttgtgttg tacccatgat gtatcaggat ttggaatggc
      481 atgatgtacc tttctggagc tatttctgtc agattagcga cagtacaaca agttatggtt
      541 cttatagcgg tgccgtgcca aacgaaaaaa ttacatgggg caaattagat attgacaccc
      601 caaaatttat tgtagaaagt gatgcttcta ttgttgcacc gctgatgttt gcctggatat
      661 tagagtggta ataatatgtt atgaatgcag ccgttaaata caccctggca ggcattgccg
      721 cagttatatt aggatttgta agcttaggta ttattcatcc tgaaattgaa tatgagtcga
      781 gggtgcagat tagcgcaccg gtaacaaaag cattcgatat ttttattgat acttcaatga
      841 tgaaacactg gatgccggga ttcgtttcat ttaaaccatt agagggtaaa atgcttacaa
      901 aaggttctaa acaattattg gtcttgaacc tcaacaacga acaaattgag attactgaaa
      961 cggttgaaga ttacagctat ccgaaatttt ataaatttca tgcgcaaaat acattactga
     1021 attcaaccaa cacaataatt tttgaaggcg attcaattca ttgtagtttg actgtaaaaa
     1081 caaaagcaag tggtaataat tttatgttac gttcaatgtt tgtatttact gaacccatct
     1141 ttaaacaaca acaggacagc acatatacgt tactaaaaag aactattgag tcacatttag
     1201 ataatcacaa catcaatcaa taattgtaat taaattttat tttaaaaaat cattaaatta
     1261 tcagtcagac atgaatagta gaattttaaa ctttctcttg ctattactga tttcaacctc
     1321 tgtaaacgcg caatcagata cagaactgaa atcaaaccta aaacaacaca tcagcatatt
     1381 agccagtgat aaatttgaag gacgtgaacc gggaaccaag ggtgaacgtt tatcttatga
     1441 atacattaca caacaattta agaatattgg tttgcaatca ttaggaagta atggttattt
     1501 acaatcattt gagttttctg ccgggacaaa aaatggagtt aaaaacaaat tgaccatagc
     1561 cggaaaaaat ttaaaatcag attccgaata ttatgtgtta ccgtattctg cacttcaggc
     1621 aaccgcagag ggcaaatcaa taaaaattgg cagtgggttg ttatcaaaag atttgggcag
     1681 aaacgattat gaggggttaa ctgttttgaa aggcaggata gcagtgatgg attatgctac
     1741 tccggaggga aataatccgc atagtaagtg ggctgaggca tcagacttac gtaagagaat
     1801 agaaattgcc gtatcaaaag gcattagtgg attgattatt tataattcaa atgattcaat
     1861 aaaaaatcct gaaaaaaact cggaaataaa aatcacacca tttgcaattc ctgttgtatt
     1921 tataaaatca aatgatgcca cattactaaa agataatcag caggtaacta tagcaacaga
     1981 tattgaaaaa ataatgcact ccggtcataa tgtaatcgga tacattgata accgtgcaga
     2041 aaatacaatt gtgattggag cacattatga tcacttgggg tatggcggac atgaatcact
     2101 ctacagaggt aagcctgaaa tacataatgg tgctgacgat aatgcaagcg gaacatcaac
     2161 attaattgag ctggcaagaa aattaaattc agcatcatta aaaaaataca actacttgtt
     2221 tattgctttt tcaggagaag aaaaaggact cctgggttca aactattttg taaaacatcc
     2281 aacaattgat ttgaaaaaag taaattatat gttgaatatg gatatggttg gcagactgaa
     2341 acctgaagaa catattttac tcataaacgg tgccggcact tctccttcat gggatagttt
     2401 attaaacaac attaaagtag attctctcag aataaaaact acagcttcag gagttggacc
     2461 aagtgaccac acttcatttt atctggaagg aattcctgta ctgcattttt tcagtggcac
     2521 acatagcgat tatcataaac ccagtgatga cgagcctttg ataaattaca atggtatggt
     2581 ttcaattttt cactttatgt acgacctgat tgaacaaacc aacaatttac caaagcttac
     2641 ttttacaaaa accaatgatt caagtaatga agatgcgcca cgttttaaag taacattagg
     2701 tgttgttcct gactatgctt ttgaaggtag cggtatgcgc attgacggag ttacagaagg
     2761 aaaacctgct gcaattgccg gatttttaaa aggcgatgtg gtaatacaga tgggtgaaga
     2821 aaaagttgct gatatgatga gctacatgaa agcattaagc aaattcaaaa aaggtgatac
     2881 tgtaaaagta aaagtacaac gaggaaaaga tgttgtagaa aaagaagtaa ccttctgata
     2941 aattattaaa gagcatgaca caacgaacca ttcactttga agaaaaacaa tatttgggac
     3001 tgaatcacta cagtctgctt agaagtatgg tattggcact cttttgtttt gtggcttact
     3061 atgttacaga aaaccgtgaa aataatgcag agttactctt ctttggtggt gtagcgttta
     3121 tggtaatttc attactttct atgtttatca aacacctgca cactagggtt tatgattcat
     3181 gtattgagtt agatggactc tggacaatgc gtaaagtaaa aattgatctc agtacaattg
     3241 ttgatgttca aaaaatcccg tatagcaact acattatgaa tactcctgta tataatctgc
     3301 acagaaatgg cagtatttat ttttatacat caggccgttc tgcaataaaa attacggaca
     3361 aggatggtat gaactatatt atcggcacac aaaagcagga agagttttta cgaattctta
     3421 acgaccttat caaaaaataa atgcccgata aaaatcgggc atttacacat tgttttacaa
     3481 ctgcattact ctttaggttt gtctttcggc ttttccattt ctgttgcctt gtgtgaatcc
     3541 ttttgaggtg catctttttt tacttcttta tcagcaggtg ctgactcatg attggcaggt
     3601 gctgcctgaa ttgttgcagg cgcatctttc ttaacttctt tttgaggagt tgtggcctgc
     3661 tcactcttta caggttgagc ctgatcttct gccttttttt ctgtttgggc attggcgcaa
     3721 tatgttgctg ccactaccgc taatgtaaga actgtttttt tcattgttat ttgatttaaa
     3781 gaatttacaa caaacataaa caaaaatcct aaatctgcaa cttatccatt ggctcggata
     3841 gcttctacag gatcgagctg tgatgctgaa tatgcaggga tgaatcctga aaccaaccct
     3901 attaaagctg aaataaacaa tccaagcata atattatgtc ctgtaaggct tagtggataa
     3961 tcaaaggcat gggacacaaa catggctaaa aaataaataa tgctcaagcc tatcaaccct
     4021 cctatcagac aaagtacaat agcttcaaac aaaaactgca tcagaataaa ataattcttg
     4081 gcaccaagag atttctgaat acctatctga ctggtacgtt ccttcactca atcagtcaag
     4141 tttgttgagc aataacatca ccaatatttt cagcgcgct
//
LOCUS       DAOOOI010000140         4143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3860_length_4143_cov_10.197407, whole genome shotgun sequence.
ACCESSION   DAOOOI010000140 DAOOOI010000000
VERSION     DAOOOI010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3860_length_4143_cov_10.197407"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKX59_10775"
     CDS             <1..231
                     /locus_tag="PKX59_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91383.1"
                     /translation="FENPDEKYRALCTQYICSYEKNLSQNFENIKSDLLAIRKAFPNP
                     ATYSIESELMLPLEETFLPLAKRTLMKYVNAH"
     gene            373..3840
                     /locus_tag="PKX59_10780"
     CDS             373..3840
                     /locus_tag="PKX59_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91384.1"
                     /translation="MIRLPLVFFLFLSVTLGYGQSLSNLRTKTIAVNSDTLHLDSLSI
                     VPGSFHLADSALLDISCMKVLYEKGILIINKSCNAKISDSLKISYRVFPVNFSQPHSN
                     RNLSIIKQKYSGQYNPFAYDNSENNTQSIFNFDGLNKSGSISRGISFGNNQDVIVNSG
                     LNLQLSGTLSDRVEILAAITDNNIPIQPEGNTQQLQEFDKVFVQLSRDSTRLIAGDFE
                     ISKPYGYFMNMYKKAQGAMVSSSFFIDEKSKWVMKTTDAAAISKGKFARNVFNGMEAN
                     QGPYRLTGAENETYIIVLSGTEKIYLNGVLLTRGKENDYVIDYNTAQITFSARRLITK
                     DSRIVAEFQYSDKNYVRTLLYSSNTFESKWAKFHVNYFTEQDNKNQPLFQDLNEDKRQ
                     VLANAGDNLQNAYFLNADSVGFNRNEVLYQKDTVTIDGIHYNYFTYSIDSTRAFYRLG
                     FSNVGQGRGNYRLLNSNVNGKVYEWIAPVNGQPQGDYEPVRLLIAPRRQQLLTAGGDV
                     GISKNTNVSVEGAFSKNDVNLFSRKDKSNDAGYALHEAVNHSMPVFKQDSSWKWVSVV
                     SAELTDKNFRPLENYRDVEFIRDWNLTSSFDTLNETFLSIKTGLERNVAQNIFYQLKY
                     FTKGNQYKGLMNTISANYNWKKYIIQYNGSLLNTTGAEANTVFLRSYVDAGKSFNKLK
                     AGYRQENERNRKSASIGDSLMLNSFGHDKAGVYLASADTGRWTYKTEVSRRWDYLPST
                     NELKLSTTADQADADIAFIAGRGKKITLGSTYRTIQVNRKEITSQKKDESFLGKLEIA
                     LQFLRGGITSNTYYEVGAGQEQRQAYSFIKVAAGAGVFEWNDYNGNGIAELNEFEVAA
                     FQDRAEYIKIFTPTNDYVRIQTNQLSEVISVNPSAFIKNKQGKTRLINRFALQSAIRF
                     DNKLLSGKWADGLNPFGYNINDTSLLITNSTLRNTLFFNRSSSVFAADFNYDNISNKS
                     LLANGTEGRNLTSWLLNTRTYFKRVYGLTTALKYETKQNSSVFTNRNYEIAGYSIEPR
                     FSVQPGTVFRTTLIYKYQDKQNVIELANEHATITTIGAECKYSSFKNGVASLGMNIIS
                     IGYNADVNSPLAFEMLEGLKKGRNYTWEISLQKNLTGNLQINIGYNGRKPQGDKIIHT
                     ANVQARAIF"
     gene            complement(3846..>4143)
                     /locus_tag="PKX59_10785"
     CDS             complement(3846..>4143)
                     /locus_tag="PKX59_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91385.1"
                     /translation="KIKTCFTILENTVATSGSKDIYIRILSPDGVVMTNNTETFNVAG
                     QSSLYSLKESFDYENREMNMCVYWEKGTAFSPGKYTVEAYCEGSLIASTPVVLK"
BASE COUNT         1416 a          704 c          839 g         1184 t
ORIGIN      
        1 tttgaaaatc cggatgagaa gtatcgcgca ctttgcacac agtatatttg ttcgtacgaa
       61 aaaaacctgt cgcagaattt cgaaaatatc aagagtgatt tattggccat tcgcaaagca
      121 tttccaaatc cggcaactta tagtatagag tctgaactaa tgctgccatt ggaagaaact
      181 tttctgcctt tggcaaaaag aacattaatg aagtatgtga atgcacatta acttgtggta
      241 taaccgacta taaaaagaaa gtattgctgt tgtttcagat acaagagaag atttaagcct
      301 gtttgttcaa tatagtggca taacctgtcg ttaacaagca gatgcatttg ctatttttag
      361 cacttcaaaa gtatgattcg actgccgctt gttttttttc tttttctgag tgttacactt
      421 ggctatggac agtcgctgag taatttacgg accaaaacta ttgctgttaa ctcagataca
      481 ctgcatcttg attcgctttc cattgtgccc ggctcatttc atttggccga ttctgcttta
      541 ttggacatat cgtgtatgaa agtgctttac gaaaaaggaa ttttgattat taataaatca
      601 tgtaatgcaa agatttcaga ctcactaaaa atatcctatc gtgtttttcc ggttaatttc
      661 agtcaacctc acagtaaccg gaacctgagt ataatcaaac aaaaatattc tgggcagtac
      721 aatccttttg cttatgacaa ctcggaaaac aacacacaaa gtattttcaa ttttgacgga
      781 ttaaataaaa gcggaagcat ttcaaggggc atcagctttg gcaacaatca ggatgttatt
      841 gtcaattcag gattaaatct tcagttgtcg ggaacattaa gcgacagggt agaaattctt
      901 gcagcaatta ccgataacaa tatccccatt caaccggaag gtaacacaca gcagttacag
      961 gaatttgata aagtttttgt tcagctttcg cgcgacagta cacgattgat tgccggagat
     1021 tttgagattt caaagcctta cggatatttt atgaatatgt ataaaaaagc acagggcgca
     1081 atggtgtcat cgtcattttt tattgatgaa aaaagtaagt gggtgatgaa aactaccgat
     1141 gccgcagcta tttcaaaagg gaagtttgca cgtaatgtgt ttaatggcat ggaagccaat
     1201 caggggcctt acagacttac aggggcagag aatgaaacct acatcattgt tttgagtggc
     1261 acagaaaaaa tttatctcaa tggtgttttg ctgacaagag gtaaggaaaa cgattatgtg
     1321 attgactaca acactgcaca aataacattt tctgcaagac gacttatcac aaaagactca
     1381 agaatagtag cagagtttca gtattcagat aaaaattatg tacgtacact tctgtattcc
     1441 tcaaacacct ttgagtcaaa gtgggcaaag tttcatgtca actatttcac ggagcaggat
     1501 aataaaaacc aacccctgtt tcaggacttg aatgaggata aaagacaagt gcttgccaat
     1561 gccggtgaca atttgcagaa tgcctacttc ctcaatgccg acagcgttgg gtttaaccgc
     1621 aatgaagttt tatatcaaaa agatactgta acaattgatg gtattcatta caattatttt
     1681 acctattcca tagattcaac aagggcattc tatagattag gattcagtaa tgtagggcaa
     1741 ggaagaggta attaccgtct gctaaacagc aatgtaaatg ggaaggtata tgaatggatt
     1801 gctccggtaa atggtcagcc acagggcgat tatgaaccgg tgagattatt gatagcacca
     1861 agacgacaac aattgcttac tgcaggagga gatgttggta tcagtaaaaa tactaatgtt
     1921 tctgttgaag gggcattcag taaaaatgat gttaatttat tttctcgaaa agacaaatct
     1981 aatgatgcag gatatgcact gcatgaagca gtcaatcatt caatgcctgt ttttaaacaa
     2041 gatagttcat ggaaatgggt ttctgtggtt tctgctgaat taacagataa aaatttcaga
     2101 ccattggaga attacagaga tgttgagttt atacgcgact ggaacctgac atcttcattt
     2161 gacacactga atgaaacttt cttgtctatt aaaaccggat tagaaaggaa tgttgcacag
     2221 aatatttttt atcaactgaa atattttaca aaaggaaatc agtacaaagg attaatgaat
     2281 actatcagtg ctaattacaa ttggaaaaaa tatattattc aatataacgg cagtttactc
     2341 aatacaaccg gtgcagaggc aaacactgtt tttttaagaa gttatgttga tgccggaaaa
     2401 agttttaata aacttaaagc aggatacaga caggagaatg aacgtaatag aaaaagtgct
     2461 tcgattggtg atagcctgat gctaaacagt tttggtcatg ataaggcggg tgtttattta
     2521 gcatctgcag ataccggcag gtggacttat aaaacagagg tatctcgcag atgggattat
     2581 ctgccaagta ctaatgagct taaactttca accactgccg atcaggccga tgcagatatt
     2641 gcatttattg ctggaagagg aaagaaaatt actttaggca gtacatacag aacaattcag
     2701 gtaaacagaa aagaaattac aagtcagaaa aaggatgaaa gctttctggg aaaattagaa
     2761 atagcattac agtttttaag aggtggaata acaagcaata cctattatga ggttggggca
     2821 gggcaggaac aaagacaggc ttactcattt attaaagttg cagcaggggc gggtgttttt
     2881 gagtggaacg actataacgg taacggcatt gccgaactta atgaatttga agtagctgca
     2941 tttcaggaca gagcagagta tataaaaata tttacgccaa caaacgatta tgtcagaatt
     3001 caaaccaatc agctaagtga agtaatcagt gtcaacccat cggcattcat aaaaaacaag
     3061 cagggcaaga ccagactgat aaacaggttt gctttgcaat cggcaatacg atttgataac
     3121 aaactgcttt ccggaaaatg ggcagacgga ctgaatcctt ttggttacaa tattaacgac
     3181 acatcattgc tcatcaccaa ctctactttg cgaaacacat tattttttaa tcgcagctca
     3241 tcagtgtttg ctgccgattt taattatgat aacatcagca ataaaagttt gcttgccaat
     3301 ggcactgaag gaagaaattt aacatcatgg ctgttaaata caagaacata ttttaaaaga
     3361 gtgtatggtc taacaacagc gttgaaatat gaaaccaaac aaaacagttc ggtttttaca
     3421 aacaggaatt atgaaattgc aggctattcc attgagccgc gttttagcgt gcagccggga
     3481 acagttttca gaactacact catctacaag tatcaggaca aacaaaatgt aattgaattg
     3541 gcaaatgaac atgcaacaat cacaaccata ggtgcagagt gtaaatacag cagctttaaa
     3601 aatggtgtag catctttagg aatgaatatt atcagtattg gatataatgc agatgttaat
     3661 tcaccattag ctttcgaaat gcttgaggga cttaagaaag gaagaaacta tacatgggaa
     3721 ataagccttc agaaaaatct tacaggcaac ctgcaaatca atattggata taacggacgc
     3781 aagccgcagg gtgataaaat tattcacact gccaatgtgc aggcaagagc aattttctaa
     3841 gaaaattatt tcagaacaac aggagtagat gcaatcaggc taccctcaca atacgcttca
     3901 acagtatatt ttccggggct aaaggcagtt cctttttccc aataaacaca catattcatt
     3961 tcgcggtttt cgtagtcaaa agattctttt aatgaataaa gtgaagactg tccggcaacg
     4021 ttgaatgttt ctgtgttgtt ggtcatcacc acaccatcag ggctaagtat tctgatgtaa
     4081 atatctttag agcctgatgt ggcaacagtg ttttcaagaa ttgtaaagca ggtcttgatt
     4141 ttt
//
LOCUS       DAOOOI010000141         4053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_3945_length_4053_cov_9.746373, whole genome shotgun sequence.
ACCESSION   DAOOOI010000141 DAOOOI010000000
VERSION     DAOOOI010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3945_length_4053_cov_9.746373"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKX59_10790"
     CDS             <1..308
                     /locus_tag="PKX59_10790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996305.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOZ91386.1"
                     /translation="SDLDAATKNVIDKGARNVEILKQGQYSPLRVEQQVAIIYLGTKG
                     LLRNVDVKKVREFETNYLSVLEATQKTILDRLKKGEFDDSITSVLEKVAADLAPKFS"
     gene            326..1204
                     /gene="atpG"
                     /locus_tag="PKX59_10795"
     CDS             326..1204
                     /gene="atpG"
                     /locus_tag="PKX59_10795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOZ91387.1"
                     /translation="MANLKEVRLRIASVNSTQQITKAMKMVSAAKLRRAQDAIMQMRP
                     YAAKLREILENLSQSLSSDGSPFTKVRAANNILIVSISSNRGLAGAFNSSVIKATSRL
                     INETYQKQTKEGKVKVLAIGRKSGDFFAKNQNVYAGNENSLYNALNFTNTAVIAERIM
                     DEFAKEKWDRVILVYNQFKNAAVQILQTEQLLPIIPPATQEKSKTVSDYIFEPNKESI
                     VSDLLPTAVKTQLFKAVLDSHASEHGARMTAMNKATDNAGELIKDLRLQYNKARQAAI
                     TNEILEIVAGAEALNG"
     gene            1301..2425
                     /locus_tag="PKX59_10800"
     CDS             1301..2425
                     /locus_tag="PKX59_10800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ91388.1"
                     /translation="MLFLLLIPALYAFLTFRWMIGFNAIADKKQTVQQYQINNFFSIV
                     IAVRNEQANLERLLHSLAELNYKKSDFEIIIVDDHSEDGTATMATQLLDLFKLNGTVV
                     SLQNSKGKKAAIAEGVKMCKAEVIITTDADCIFNPDWLVTINNEFNLSKPDMMLLPVV
                     LQGKGFLSNMQQTESLGLAALTFGSLAIGKPLMCNGANLVFKKEAYLQMNNTEMRHDV
                     ASGDDTFFMLSLFAKNKQSIRALVSGKVVVTSDAQTGLSELISQRIRWASKVKHCKQG
                     YIKTTGLFITLFSLVQYAAFLVIILFPVMSPVGLVSAFTIAVKWICDFIFIQNAASKL
                     NQKFYPGAFILMQLLYPVYTFVIGVLSLSDHYHWKGRQYH"
     gene            2496..3026
                     /locus_tag="PKX59_10805"
     CDS             2496..3026
                     /locus_tag="PKX59_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOZ91389.1"
                     /translation="MALIKSVNGIIPKFGKNFYAAENATIVGQVVMGDDCSVWFNAVV
                     RGDVHSITIGNKVNIQDGAIIHCTYQKAPTVIGNNVSIGHNAIVHGCTLEDNVLIGMG
                     AIVMDHAIIKTNAIIAAGSVVLENTVVESGCIYAGVPAKKVKEVSPEHFKDLNERIAN
                     NYVMYSGWFKKDTDEK"
     gene            3016..>4053
                     /locus_tag="PKX59_10810"
     CDS             3016..>4053
                     /locus_tag="PKX59_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91390.1"
                     /translation="MKNRVILFWLFLNICFLFFSCATPEKKESVQQVDSSTVVQTHFT
                     KIKLEPGKLLSNVKSESNSNVSFSVYAPSEYSDTTLLPIIYFFDPHADGNLPLEKYKS
                     IANTFNLILVGCNSIENGMPFEQSQQIANILFSDCQNRLPINTQQQIIAGFSGGSKVA
                     CNLAMNQHFFTGLIACSGALFENGKFDKNMSVVSVAGINDFNYHEMVALEKSVDVTHH
                     AFIETDSTHQWPGTSDMYDATLFTLMQMVKQHKLQMQSSVVENFYHTWLKKINDSANK
                     SSPGKQFDQLQFGIMCFDGLTDVTSLKSKLKEIENSDNLRKWRMRSQNMQYQEKEMQK
                     MLRDAFSNQNLA"
BASE COUNT         1370 a          654 c          771 g         1258 t
ORIGIN      
        1 gttctgacct tgatgctgct actaaaaacg taatagataa aggtgcccgt aacgttgaga
       61 ttcttaaaca aggacagtac tcaccattgc gtgttgagca gcaggttgcc ataatttatc
      121 ttggaacaaa aggcttgtta cgtaatgttg atgttaagaa agtaagagag tttgaaacta
      181 attatctgag cgttctcgaa gcaacacaaa aaacaattct tgatagattg aaaaagggtg
      241 aatttgatga cagcataact tctgtacttg aaaaagttgc tgcagacctt gctccgaaat
      301 tttcttgata attcttaatt aaaaaatggc caatttaaaa gaagtacgac tgcgcattgc
      361 ttcggtaaat tctactcagc aaattaccaa ggcaatgaaa atggtttctg ctgctaaatt
      421 acgcagagca caggatgcaa ttatgcagat gcgtccttat gctgccaaac ttagagagat
      481 acttgaaaac ctttcacaaa gtttgtcatc agacggaagc ccattcacca aagtgcgcgc
      541 agcaaataat attctcatcg tttctatttc ttcaaaccgt ggtcttgccg gtgcatttaa
      601 ctcatctgta ataaaagcaa catcaagatt gattaatgag acctatcaga aacagactaa
      661 agaaggtaaa gtaaaagttc ttgctattgg aagaaagtca ggtgactttt ttgcaaagaa
      721 tcaaaacgtt tatgccggta atgaaaacag cctgtacaat gcattgaatt ttacaaacac
      781 tgctgtaatc gctgaacgca ttatggatga gtttgcaaaa gaaaagtggg acagagtgat
      841 tttggtttat aatcagttta aaaatgctgc tgtacaaata ttgcagacag aacaactgtt
      901 gccgataatt cctcctgcta cgcaagaaaa aagcaaaaca gttagtgatt atatttttga
      961 acccaataaa gaatcaattg taagcgactt attgcctact gccgttaaaa cccaattgtt
     1021 taaagcagtg cttgattctc atgccagtga acatggtgcc cgaatgactg ccatgaataa
     1081 ggccaccgac aatgcagggg aattaattaa agaccttcgt ctgcaatata acaaagccag
     1141 acaggcagct atcacaaacg aaattcttga gattgtggca ggtgcagaag cactcaacgg
     1201 ataaaattcc ttttaaaaat ttaaaaccgc agcaatgcgg ttttttttat gcctaaattt
     1261 taaagtggca atataatttt tcctatcctt acatttgcct atgctttttt tgttactcat
     1321 tccggcattg tatgcttttc tgacgttcag atggatgatt ggctttaatg caatagctga
     1381 taaaaaacaa acggttcaac agtatcaaat caataatttt ttttcaattg ttattgctgt
     1441 tcgaaatgaa caagccaact tagaacgatt actacattcg ttggcagaac taaactataa
     1501 aaaatctgat tttgaaatta ttattgttga cgatcattct gaagatggaa ctgcaacaat
     1561 ggcaacacag ttactcgatt tattcaagct aaatggtact gtcgtttcac tacaaaattc
     1621 aaaaggtaaa aaggcagcta tagcagaggg agttaaaatg tgtaaagcag aagtgataat
     1681 tacaactgat gcagattgca ttttcaatcc ggattggctt gtaacaataa acaatgaatt
     1741 taatttatca aagccggaca tgatgctatt gcctgttgtc ttacagggaa aaggtttctt
     1801 atcaaatatg caacaaacag aatcattagg tttagctgct ttaacttttg gttcattagc
     1861 tattggaaaa ccattaatgt gcaatggcgc caatctggtt tttaagaaag aagcttattt
     1921 gcaaatgaac aatacagaga tgcgacatga tgtagcctca ggagatgata cattttttat
     1981 gctgtcgttg tttgcaaaga ataaacaaag catacgtgct ttagtttccg gaaaagttgt
     2041 agtaacatct gatgctcaaa ccggattgtc agaattgata agtcaacgaa ttagatgggc
     2101 atctaaagtg aaacattgca aacagggtta tattaaaaca accggtttgt ttattactct
     2161 attttcttta gtacagtatg ctgcatttct tgtaataata ctttttccag taatgagtcc
     2221 cgttggttta gtgtcagcat ttacaattgc agtaaaatgg atttgtgatt ttatttttat
     2281 tcagaatgca gcatcaaagc ttaatcagaa attttatccc ggtgctttta tattaatgca
     2341 gttgttgtat cctgtttata cttttgttat tggtgtttta agtttgtctg accactatca
     2401 ttggaaggga aggcaatatc attgaagaat aaaatttgtt aacagacaat ttcattttat
     2461 tgaatggtaa ttgtagtttt gaagaaaaat aattcatggc actgataaaa tctgttaatg
     2521 gcattatacc aaaattcgga aagaattttt atgctgccga aaatgctaca atagttggcc
     2581 aggtggtgat gggtgatgat tgcagtgtgt ggtttaatgc cgttgttagg ggtgatgtac
     2641 attcaataac aattggcaat aaagtaaata ttcaggatgg tgctattatt cattgcacat
     2701 atcaaaaagc accaacagtt attggaaata atgtttctat tggtcacaat gccattgttc
     2761 atggctgtac acttgaagat aatgtgctga ttggtatggg tgcaattgta atggatcatg
     2821 caatcattaa aacaaatgca ataattgctg caggttctgt tgtgctggaa aacacagtgg
     2881 tagaatcagg ttgtatctat gcgggagtgc ctgcaaaaaa agtaaaagaa gtaagccctg
     2941 aacattttaa agatttgaat gagcgtattg ctaataacta tgtaatgtat tccggttggt
     3001 ttaaaaaaga cacggatgaa aaatagagtt atactttttt ggcttttttt aaacatttgt
     3061 tttctttttt tctcatgtgc aacacctgag aaaaaagaat cagtacaaca agttgattct
     3121 tcaacagtag tgcaaacaca ttttaccaaa ataaaattag aacccggaaa acttctttcc
     3181 aacgtcaagt cagagagtaa cagtaatgta tctttttctg tttatgcacc atccgaatat
     3241 agcgatacaa cattacttcc tataatttat tttttcgatc ctcatgccga tggaaatttg
     3301 ccattagaaa aatacaaatc aattgccaat acttttaatc tgatattggt tggttgcaac
     3361 agtattgaaa acggaatgcc ttttgaacaa tcacaacaga ttgccaacat tcttttttct
     3421 gattgtcaaa acaggttgcc tataaacacg caacaacaaa ttattgcagg attttcaggg
     3481 ggtagtaaag tagcttgcaa tcttgcaatg aatcaacatt tttttacagg attgatagcc
     3541 tgttctggag ccttgttcga aaatggcaag tttgacaaga acatgagtgt agtttcagta
     3601 gcaggtataa atgattttaa ttaccatgaa atggttgcat tagaaaaatc tgttgacgta
     3661 acacaccatg cttttattga aacagacagc acacatcaat ggccgggcac aagcgatatg
     3721 tatgatgcca cgctctttac actgatgcaa atggttaaac agcataaatt acagatgcag
     3781 agttctgttg tagaaaattt ttatcatacc tggttaaaga aaattaacga ttcagcaaac
     3841 aaatcttctc ccggaaaaca atttgatcag ttacaatttg gaattatgtg ttttgatgga
     3901 ttgacagatg taacaagtct aaaaagtaaa cttaaagaaa ttgaaaactc cgataaccta
     3961 cgtaagtggc gtatgcgaag tcagaatatg cagtatcagg aaaaggaaat gcagaagatg
     4021 ttacgcgatg ctttttcaaa tcaaaacctg gca
//
LOCUS       DAOOOI010000142         4002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4017_length_4002_cov_9.157335, whole genome shotgun sequence.
ACCESSION   DAOOOI010000142 DAOOOI010000000
VERSION     DAOOOI010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4017_length_4002_cov_9.157335"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>208
                     /locus_tag="PKX59_10815"
     CDS             <1..>208
                     /locus_tag="PKX59_10815"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOZ91391.1"
                     /translation="KKLFFAALILCATTAVNAQDDTKKAPAANPNAPDISFESETHDF
                     GTIPYSGNGTYEFKFTNTGKEPLV"
     assembly_gap    209..297
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <298..495
                     /locus_tag="PKX59_10820"
     CDS             <298..495
                     /locus_tag="PKX59_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOZ91392.1"
                     /translation="SVHYDTKRPGPFTKTVTVSSNGKTAEKVLTIKGNVETKEQTESG
                     TPVKKDNTMTPKETTTSPFGN"
     gene            546..995
                     /locus_tag="PKX59_10825"
     CDS             546..995
                     /locus_tag="PKX59_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOZ91393.1"
                     /translation="MKKTILLSALAIGMATATFAQEKAAAVQVAPAENKNQADFKFET
                     LEYNYGTIKQGDKVNYTFNFVNTGKEPLIITNAQGSCGCTVPEWPKEPIKKGEAGKIH
                     VTFNSAGKMGLQDKTITITSNAKTSPVVLHIKGNIEAAAATQEAPKN"
     gene            1084..2316
                     /locus_tag="PKX59_10830"
     CDS             1084..2316
                     /locus_tag="PKX59_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008504774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOZ91394.1"
                     /translation="MPSISEKGKHMPPSPIRKLVPFADKAKAEGKKIYHLNIGQPDIE
                     SPKVMLDAIRSIDLKVIEYSHSAGIESYRRKLISYYKKYNININFEDILITTGGSEAI
                     EIAMMTCLNEGDEIIIPEPFYANYNGFSTQAGVTVVPVFSSIRNGFALPSHDEFEKVI
                     TPRTKAIMICNPSNPTGHLYSKEELQSLRDLALKHDLYLFADEVYREFCYDNNSYFSI
                     MHLEGLDENIILLDSISKRYSACGARIGAMISKNKKVMSTAMKFAQARLSPPTFGQIA
                     AEAAIDTPQEYFDNVKAEYVARRNFVVDKLNSMPGVFCAKPSGAFYCMAELPIDDSDA
                     FCQWLLEKFSYQGQTVMLAPATGFYSHAELGKKEVRIAYVLNLDDLKNAMICLNEALK
                     VYPGRVESKSNSVTINQG"
     gene            complement(2386..2976)
                     /locus_tag="PKX59_10835"
     CDS             complement(2386..2976)
                     /locus_tag="PKX59_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOZ91395.1"
                     /translation="MIKIFAGNKTFHFTNNADGLSEDVIKFENSIHKDELKKIFSDSK
                     SIEIVCLCHNAESGFKEFTKMFRNIDAAGGVVYNHEKKLLLIHRLGKWDLPKGKMEDG
                     ESPETSAMREVCEETGVCNLEIIKPLRDTFHIYQQNGEYILKRTYWFEMITSSATNLL
                     PQTEENITEVKWMTKEEVNAAMQFSYESIRYLLSQT"
     gene            3021..3656
                     /gene="pyrE"
                     /locus_tag="PKX59_10840"
     CDS             3021..3656
                     /gene="pyrE"
                     /locus_tag="PKX59_10840"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOZ91396.1"
                     /translation="MKDDTASNIADFLLQIKAIKLQPDEPFTWASGWKSPIYCDNRVA
                     LSNPRIRTYIRQELVKAIERNYGRPDAIAAVATGAIAQGALVSESMGLPFIYVRPEPK
                     QHGAKNQIEGDISQIHSVVVVEDLISTGKSSLNAVAALRNSGIIVKGMVSIFNYGFDV
                     AKESFKNAQCPLYSLSNYDAMIIKALQSKYINESQLESLKDWREHPDSWNK"
     gene            3664..>4002
                     /locus_tag="PKX59_10845"
     CDS             3664..>4002
                     /locus_tag="PKX59_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOZ91397.1"
                     /translation="MTRIESDEWVIDKPANEVFEFLSNCNNLQQLMPEQVTNWQSTTD
                     DCSFTIAGMASLGMEIKERNPGQNIRVERKGKAPFDFVMNYEMQTVDAGKTKLKIAFD
                     ADLNPMLKLMA"
BASE COUNT         1325 a          640 c          752 g         1196 t
ORIGIN      
        1 tgaagaaatt attttttgca gcactgattt tatgtgcaac aactgcagta aatgcacagg
       61 atgatacaaa aaaagcacct gctgctaatc ctaatgcacc tgatattagc tttgaaagcg
      121 aaacgcatga tttcggtaca ataccttata gtggtaatgg tacctatgag tttaaattta
      181 caaatacagg taaagaacca ttagtaatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagt
      301 gttcattatg atactaagcg tccggggcca tttactaaga ctgtaacagt ttcttctaat
      361 ggaaaaacag cagaaaaagt gttgactatt aaaggaaatg ttgaaacaaa ggagcaaaca
      421 gaaagtggaa ctcctgttaa aaaagataat acaatgactc caaaagagac caccacaagt
      481 ccatttggta attaatcaca taaaataaat attaaacaca caaatcaaca atcaaatttc
      541 aaaaaatgaa aaaaacaatt cttctttcag cattagctat cggtatggca actgctacat
      601 tcgcacaaga aaaagcagca gcagttcagg ttgctcctgc tgaaaacaaa aatcaggctg
      661 actttaagtt tgaaactttg gagtacaatt atggtactat taaacaaggt gacaaggtga
      721 attacacttt taactttgtg aacacaggaa aagagccttt aattattact aatgcacaag
      781 gatcttgtgg ctgtacagta cctgagtggc caaaagagcc aattaaaaag ggcgaagccg
      841 gaaaaatcca tgtaactttc aattcagcag gtaagatggg tcttcaggat aaaactatta
      901 caattacttc taatgcaaaa acaagccctg ttgttttaca cattaaagga aatattgagg
      961 ctgcagctgc tacacaagaa gcaccaaaga attaactaag gtaattagat taattataat
     1021 tttgcaggga cgattgaatt caatcgtccc ttattttttg tatgtaatta atataaaaac
     1081 tgcatgccat ctatttcaga aaaaggcaaa cacatgcctc cttcaccaat cagaaaattg
     1141 gtgccttttg ccgataaagc caaagcagaa ggaaaaaaaa tatatcacct caatattggt
     1201 cagcctgata ttgaatcccc aaaagtaatg ctcgatgcaa tcaggagtat agaccttaaa
     1261 gttatagagt acagccattc tgctgggata gaatcatatc gaagaaagtt gatttcgtat
     1321 tacaagaagt ataatatcaa tattaatttt gaagatattt taattacaac cggtggatca
     1381 gaggcaattg aaattgccat gatgacctgt ttgaatgaag gtgatgagat tattattcct
     1441 gaaccgtttt atgccaatta taatggtttt tccacgcaag ccggagttac tgtcgtacct
     1501 gttttttcat ctattagaaa cggatttgca cttccatcac atgatgagtt cgaaaaagtt
     1561 attacacctc gaactaaagc catcatgatt tgtaacccta gtaatcctac tggccacctt
     1621 tattcaaaag aagagctgca aagtttgcgc gaccttgctt tgaaacatga tttgtatctt
     1681 tttgctgatg aagtttatag agaattttgt tatgataaca acagctattt ttcaataatg
     1741 catcttgaag gattggacga aaatattatt ttgttagact ccattagtaa gcgatatagt
     1801 gcctgcggtg cacgaatcgg tgccatgatt tctaagaata aaaaagtgat gagcacagct
     1861 atgaaatttg cacaagcacg tttaagcccg cctacatttg gacaaatagc agcagaggca
     1921 gctatagata cgcctcagga atattttgat aatgtaaagg cggagtacgt ggcaagaaga
     1981 aattttgttg tggataagtt gaatagtatg ccgggtgtat tttgtgccaa accaagtggt
     2041 gcattttatt gtatggccga attgcctatt gatgattcag atgctttttg tcagtggttg
     2101 ctcgaaaagt tttcatatca ggggcaaaca gtaatgctgg caccggctac cggtttttat
     2161 agtcatgccg aattgggaaa gaaagaagtg cgcattgcct atgtacttaa tttggatgat
     2221 ttgaaaaatg ctatgatatg tttgaatgaa gcattaaaag tttatccagg cagagtagaa
     2281 tcaaaatcaa atagtgtaac aataaatcag ggctaacata tttgtggctt ttgtgtttaa
     2341 atcaatcaca gaagagtttt ttaaatggaa gattttttcc tggtgttaag tttgacttaa
     2401 taagtatcgt atagattcat acgaaaattg cattgctgca tttacttctt ctttagtcat
     2461 ccatttaact tccgtaatgt tttcctctgt ttgtggtaaa agatttgttg cagacgaagt
     2521 aatcatttca aaccaataag ttctttttaa aatatattct ccattttgtt gatagatatg
     2581 aaaggtgtct cgtagcggtt taataatttc cagattacag accccagtct cctcacatac
     2641 ctctcgcatg gctgaagttt ccggtgattc tccatcttcc atttttcctt ttggcaaatc
     2701 ccatttgccc aatctgtgga ttagaagcag ctttttttcg tgattataaa caacaccacc
     2761 ggctgcatca atgtttctaa acatttttgt aaactccttg aaacctgatt cggcattgtg
     2821 gcacaaacaa acaatttcaa tgctttttga atcagaaaaa atcttcttaa gttcatcttt
     2881 atgaatgcta ttttcaaact ttatcacgtc ttcagaaaga ccatcagcat tgtttgtaaa
     2941 atgaaatgtt ttattgcctg caaatatttt aatcacctct gattttttat tttaaaaaat
     3001 aactattagg tttgtgctga atgaaagacg acacagcttc aaatatagcg gattttttat
     3061 tgcagataaa agcaataaaa cttcagcctg atgaaccatt tacatgggca tcggggtgga
     3121 agtcgccaat atattgtgat aacagagttg ctttgtctaa tccaagaata agaacgtaca
     3181 tccgtcagga gttagttaag gctattgaac gtaattatgg gcgccctgat gctattgcag
     3241 cagtggccac aggtgcaata gcacaaggag ctttggtttc agaaagtatg ggattgccat
     3301 tcatttatgt cagacctgaa cctaaacagc atggcgctaa gaatcaaatt gaaggtgata
     3361 tttcgcaaat acattctgta gttgttgttg aagatttaat ttctacaggt aaaagtagtc
     3421 tgaatgccgt agcagcttta agaaacagcg gcatcattgt taagggaatg gtgtccattt
     3481 ttaattatgg ttttgatgtt gccaaagaga gttttaaaaa tgctcaatgc ccattgtatt
     3541 cattaagcaa ttatgatgcg atgattataa aggcattgca gtcgaagtat attaacgaaa
     3601 gtcagttaga atcattaaag gattggcgtg agcatcctga tagctggaat aaataatcaa
     3661 tgaatgacaa gaattgaaag tgatgagtgg gtaatagaca aacctgcaaa tgaagttttt
     3721 gaatttttaa gtaattgtaa caaccttcag caattaatgc ctgaacaggt aacaaactgg
     3781 caaagtacaa ctgatgattg tagttttaca attgcaggaa tggcaagtct tggcatggaa
     3841 atcaaagaaa gaaatcccgg tcagaatatt agagttgaac gtaaaggaaa agcaccattc
     3901 gattttgtga tgaattatga gatgcaaact gttgatgccg gcaaaacaaa actaaaaatt
     3961 gcttttgatg cagatttgaa tcctatgtta aagttgatgg ca
//
LOCUS       DAOOOI010000143         3979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4044_length_3979_cov_10.396534, whole genome shotgun sequence.
ACCESSION   DAOOOI010000143 DAOOOI010000000
VERSION     DAOOOI010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4044_length_3979_cov_10.396534"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..478)
                     /locus_tag="PKX59_10850"
     CDS             complement(215..478)
                     /locus_tag="PKX59_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOZ91398.1"
                     /translation="MYFVYVLYSESGSRHYIGQTNDISTRLHRHNSGYEKSTLPYVPW
                     KLLCKIEKQSRSEAMILERKLKNLNTEDLRKFIQKYGIKPTGG"
     gene            complement(531..615)
                     /locus_tag="PKX59_10855"
     tRNA            complement(531..615)
                     /locus_tag="PKX59_10855"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(579..581),aa:Ser,seq:tga)
     gene            721..2172
                     /locus_tag="PKX59_10860"
     CDS             721..2172
                     /locus_tag="PKX59_10860"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOZ91399.1"
                     /translation="MKRLLLFLALGSIASLTQAKTIKALFDYKSFYAPGQGSYLEWYL
                     SVKGTSVNYKMSESGNYEASVAVAYKISQNDKVVAQDSYNLISPLTKDTLLVSDFIDN
                     KRLQLPNGKYNIELSVKDNVADAKTFTINQGFELAFSPGKTAVSDIQLIQNYSKKETG
                     NSALIKNGYEIIPRVDNFYQTHEERLIYYAETYFTNSLTDDAVAVYSYLQNAESGNTI
                     MDYGTVQRKKTSEVIPLLSEINIANLGSGNYNLVVEIKDKNNQSLALSKIFFQRQSVV
                     KDNFALNNDVSSTFVATMNDKNVLAENIACLQPISNAQQSEWIGNQLKAANIDMMKNF
                     FYDFWQRREPADPQKAWNVYKTHVDAVQQKFGSRALKGYNTDMGRVYLAYGAPSNINK
                     GEYEPNTYPYEIWRYDKINNHTNKTFVFYSKDLLARNFTLLHSDMPGEPYEAAWNMIL
                     HDRSIKAKNIDDTGQDLKGVYSGDRTNDNFNAK"
     gene            2179..3030
                     /locus_tag="PKX59_10865"
     CDS             2179..3030
                     /locus_tag="PKX59_10865"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOZ91400.1"
                     /translation="MTFLFLKFVIRCIAFLDLRILYLLADGIAFCFKYIFPYRKKVIL
                     QNLYNSFPEKSEQEIEEIAKQFYNYFANLIVETIKMSSISKEELLRRWRFTNAEVLHS
                     YYEKNRSIIAVCGHFNNWELGALALSVIDKHQMLGVYKPLTNKSWDEYFRTIRSRFGM
                     IPVPMKSILRELIKRKNEPTITTLAADQTPHRSEINYRTTFLNQDTAVFLGAEKLCKQ
                     TGHVLIYFSMHQIRRGYYDITIIPISDDPKNTAEYELTHKHLRLLENDIHAQPGNWLW
                     SHRRWKY"
     gene            3044..3328
                     /locus_tag="PKX59_10870"
     CDS             3044..3328
                     /locus_tag="PKX59_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HOZ91401.1"
                     /translation="MNVLKQFRLIAIAEGISFLVLLFIAMPLKYGADMPLAVKYTGWV
                     HGLLFVFYVYSLLQVWQQYKWNLKMMSLAMLASVLPFGTFYFEKKYLNNI"
     gene            3360..>3979
                     /locus_tag="PKX59_10875"
     CDS             3360..>3979
                     /locus_tag="PKX59_10875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019974286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ91402.1"
                     /translation="MKAAVVILNYNGAALLQQFLPSVIANTPSWCSVIVADNASTDNS
                     LSVMKKHFPDVRCIEMQQNTGFAGGYNNALQHVDAEYFILLNSDIEVTPQWIEPIIEF
                     MDKHADVAACQPKILAYHRKEYFEHAGACGGYIDYLGYPFCRGRIFNSLEKDENQYSD
                     NKEIFWATGAAMFIRSHVFKSAGGFDTDFFAHMEEIDLCWRMKHSG"
BASE COUNT         1275 a          739 c          745 g         1220 t
ORIGIN      
        1 gcttgctcgc tattgagagt gacaacggaa cctcacaagg gcgaggcttg ctcgctattg
       61 agagtgacaa cggaacctca caagggcgag gcttgctcgc tattgagagt gacaacggaa
      121 cctcacaagg gcgaggcttg ctcgccattg agagtgacaa agaaacctca caatagcggg
      181 gcttgctcgc tattgagagt gacattgtca cctctcaacc gcccgttggt tttattccat
      241 acttctgtat aaactttcgt aagtcttcgg tattcaaatt tttcaacttc cgttccaaaa
      301 tcattgcttc agacctactc tgtttttcta ttttacaaag taacttccat ggtacatatg
      361 gcaatgtaga tttttcatag ccactgttat gtctatgtaa tcgtgtgcta atatcatttg
      421 tctgacctat ataatggcga ctaccgctct cagaatataa aacgtaaaca aaatacattt
      481 tctcatttac tccttaaact aaaaaagcct cctatcggaa gcctcttttg cggagagaga
      541 gggattcgaa cccccgttgg ttttacccaa ggcggttttc aagaccgctg caatcaacca
      601 ctctgccatc tctccgggcg cgaaagtaat acaattttta attcgcaaat tttttttgac
      661 gattttacaa cactaattct taaaatcttg tctatcttta tactctaaat aattcataca
      721 atgaaacgct tattattatt cttagccctt gggtctatag catcgctgac tcaggctaaa
      781 acaattaagg ccttgtttga ttataaatcc ttctatgctc ccggtcaggg ttcgtatctg
      841 gaatggtatc tgtctgtaaa aggtacttcc gtaaactata aaatgagcga aagcggtaac
      901 tacgaagcct cagtggctgt agcttataaa attagccaaa acgacaaagt cgttgcacag
      961 gatagctata accttatcag tccgcttaca aaagacacac tcttagtatc cgattttatt
     1021 gacaataaaa gattgcagtt gcctaatgga aagtataaca tcgagttatc ggtaaaagat
     1081 aatgtggcag atgctaaaac ctttaccata aatcagggat ttgaattggc tttttcaccg
     1141 ggaaaaactg cagtgtctga tattcaattg atacagaatt attctaaaaa agaaaccggc
     1201 aatagtgctt taattaaaaa tggatatgaa ataattcctc gtgttgacaa tttttatcag
     1261 acacatgaag aacgtttaat ctattatgca gaaacctatt ttacaaactc ccttactgat
     1321 gatgccgttg ccgtttacag ttatctgcag aatgcagaat caggcaacac tataatggat
     1381 tatggaactg ttcagcgtaa aaaaacaagt gaagtaattc cgctgttgag cgaaattaat
     1441 atagctaatc tgggttcagg caactacaat cttgtcgttg aaataaaaga taaaaacaat
     1501 cagtcattgg cattatcaaa aatatttttt cagcgtcaat ctgtagtgaa agataatttt
     1561 gcattaaata atgatgtttc atcaaccttt gttgccacaa tgaatgataa aaatgtgctt
     1621 gcagaaaata ttgcctgtct gcaacctatc agcaatgcgc aacaatcgga gtggataggc
     1681 aatcagctta aagctgccaa cattgatatg atgaaaaatt tcttttatga cttttggcaa
     1741 cgcagagagc ctgccgatcc acaaaaagca tggaacgttt ataaaacgca tgtagatgcc
     1801 gttcaacaaa aattcggttc acgtgcctta aaaggttaca atactgatat gggaagagtt
     1861 taccttgcct atggcgcacc aagcaatata aataaaggtg aatatgaacc caatacttat
     1921 ccgtatgaaa tctggcgcta cgataaaata aacaatcaca ccaacaagac ttttgtattt
     1981 tacagcaaag acttgttagc acgtaatttt acacttttgc acagcgatat gccgggcgag
     2041 ccttacgaag cagcatggaa tatgattttg catgatcgca gcattaaagc taaaaatatt
     2101 gatgataccg gtcaggattt gaaaggtgtt tattcaggtg atagaactaa tgataatttt
     2161 aatgccaagt aattttaggt gacctttctc tttctgaagt ttgtaattcg atgtattgct
     2221 tttcttgatt taagaatact atatctctta gctgatggta ttgccttctg ttttaaatat
     2281 attttcccat atcgcaaaaa ggtaatcctt caaaatcttt ataactcctt tcctgaaaaa
     2341 agtgaacagg agatagaaga aattgctaag cagttttata actactttgc caacctgatt
     2401 gtagagacca taaaaatgtc gagcatatca aaagaagaat tgctcagacg ctggcgtttt
     2461 acaaatgcag aagttcttca tagctattat gaaaaaaacc gtagtatcat tgccgtttgt
     2521 ggtcacttca ataattggga gttaggagca ttggcacttt ctgtaataga taaacatcag
     2581 atgcttggtg tttacaaacc attgaccaac aaatcgtggg atgaatattt tagaaccatt
     2641 cgttcgcgat tcggtatgat accggttccg atgaaatcaa tattacgcga attgattaaa
     2701 cgtaaaaatg agccaaccat aactacgctt gctgccgatc agacaccaca ccgttctgaa
     2761 ataaattatc ggacaacttt tttaaatcag gatacagcag tatttctggg cgcagaaaaa
     2821 ctttgtaaac aaaccggtca tgtacttata tatttttcga tgcaccaaat tcgtagaggt
     2881 tattatgata ttaccatcat tccaataagt gatgacccaa aaaatactgc tgaatacgaa
     2941 ctcactcaca aacatttaag attattagag aacgacatac atgcacaacc cggtaactgg
     3001 ctttggagtc accgcagatg gaaatattaa ataacaatca acaatgaacg tattaaaaca
     3061 attcagatta attgcaattg cagaaggcat ctccttttta gttttacttt ttattgccat
     3121 gccgttgaag tatggcgcag atatgcccct agcagtaaaa tatacaggct gggtgcatgg
     3181 acttttattt gtgttttatg tttattcgct gcttcaggtg tggcagcaat ataaatggaa
     3241 tttaaaaatg atgtcgttag ccatgctggc atcggtattg ccttttggaa ctttttattt
     3301 tgagaagaaa tatctgaata acatataatg ccctttagat ttcatttctt tgcagcctga
     3361 tgaaagctgc tgttgtcata ttaaattata atggtgcagc attactgcaa caatttctgc
     3421 cttcggtaat tgcaaacaca ccgtcctggt gttctgttat agttgcagac aatgcctcta
     3481 ccgacaattc cttgtcggta atgaaaaagc attttcctga tgtacgctgt attgaaatgc
     3541 aacaaaacac tggctttgca ggaggttaca acaatgcatt gcagcatgta gatgcagaat
     3601 attttatttt actcaattca gatattgaag taactccaca atggattgag cctattattg
     3661 aatttatgga caaacatgca gatgttgcag cttgtcagcc aaaaatatta gcctatcacc
     3721 ggaaagaata ttttgaacat gcaggtgcct gtggcggata tattgattat ttgggttatc
     3781 cgttttgcag aggaagaatt tttaattcat tagaaaaaga cgaaaaccaa tattcagata
     3841 acaaagaaat tttttgggca acaggtgctg ctatgtttat cagaagccat gtttttaaat
     3901 cagcaggagg ttttgacact gacttttttg cacacatgga agagattgat ctttgctggc
     3961 gtatgaagca tagtggtca
//
LOCUS       DAOOOI010000144         3977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4048_length_3977_cov_8.376084, whole genome shotgun sequence.
ACCESSION   DAOOOI010000144 DAOOOI010000000
VERSION     DAOOOI010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4048_length_3977_cov_8.376084"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PKX59_10880"
     CDS             complement(<1..906)
                     /locus_tag="PKX59_10880"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOZ91403.1"
                     /translation="MKDIVNHNYHLFIYAVLFLGSLAFSFLINSLFLRFFFTLGIRNT
                     NDGTVIRWGSMSKPAVGGLSFYIVFLLSLATYSVFFNNNDALYNSQFMGFIMAMAIGF
                     LIGLADDAYNTKPVLKFLAQVSCAFSFIFTGTYIDLFDNMILNYLLTIFWVVGIMNSV
                     NMLDNMDGITSTVSLGVIISAVVVITMSNDINNLYLASIIGLSAGLLGFLYFNWHPSK
                     MYMGDTGSQFLGVFLAGIGIMFFWNNPYHITESLPIKKILTVAIAFLLPLVDTTVVVV
                     NRISKGKSPFIGGKDHTTHSLAYLGL"
     gene            complement(1006..1548)
                     /gene="rfbC"
                     /locus_tag="PKX59_10885"
     CDS             complement(1006..1548)
                     /gene="rfbC"
                     /locus_tag="PKX59_10885"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOZ91404.1"
                     /translation="MKIIKTALNGICILQADVFSDERGYFLESYHKEKLKNLGIEAEF
                     YQDNFSLSAKNVLRGLHFQNPPFDQGKLIKVISGSVLDVVVDIRKSSATYGKNFTIEL
                     NSEDHLMVWIPPGFAHGFVSLAENTLFYYKCTKPYNKDSEAGIIYNDPALNINWGVNQ
                     PVLSEKDKILPNFSDATILF"
     gene            complement(1555..>3977)
                     /locus_tag="PKX59_10890"
     CDS             complement(1555..>3977)
                     /locus_tag="PKX59_10890"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOZ91405.1"
                     /translation="QITPLNEEFDFKLFVTIAQRNVEWFLMFMTLSIIVWYVIVRYTP
                     PMYESAALMKLNTDNNVTKALNITRDVYQEQNNEVAGNLELIRSSIIVGKAIATLPMR
                     VTYISKGTLLENEMYKSCPFEVNFLVKDSLWMDRPFYLQFNNEKTFVLSYTPVGQRDQ
                     VDEEYEINKWIHLNGISLQINIIDFQRILKYQSQLKKNTFKFIINSDKALIKDFSSKL
                     SVIMANPDAKTINIKIKEKNATKAADMSNAISQEFLKFDVLRSREATDRILEFIDTTL
                     YRVNNDLSESEMNLERFKSNNRIITPELLASDLSDKMSQLELRLTDYTLNLLAISELK
                     KTIESEKKVDEKMLSLTGIYKTDEIKDVLSNLNILLDKREQALIMVTDKAEYVQMLSG
                     RIQRQKDYLLQSVAYEENGIKQKKAELESQYRKLNSNYLGIPEQQTEFERLQRIFNVN
                     EKFYSLLLEKKAEFSITRAGYVSKNIILEAARPAPAPYYPNKSLILSSCIMVGFVASF
                     LLIFIKYLFYNQIGSLEEIAQYTDAPLLGIVPRYKREIPISQLLVDKSPKSVIAEAFR
                     SIRTNLQFINNDDKPQLMALTSTISGEGKTFNAINLAGVISFSGKKVIILDLDMRKPK
                     IHLGFNVENNRGMSTILIGKDTIENCINHSALENLDFITAGPIPPNPSELIISPKMDE
                     LLEYLKVRYEVIIADTPPVGVVSDGIPMIQKADFPIYILRANYSRKMFIHNINKIISE
                     NKVPRLSVVLNSVDQTKLKYGYGYGYGYGYGYGYGYSYGYGYGYYSEDDKKPTGFWNR
                     IISFFIRK"
BASE COUNT         1316 a          713 c          640 g         1308 t
ORIGIN      
        1 taatcccaga taggctaaac tatgagttgt atgatcttta cctcctatga agggactttt
       61 acctttactg attctgttta caacaacaac agtggtatca actaaaggaa gtaaaaatgc
      121 aatggctaca gtcagaattt ttttgatagg aagcgactct gttatatgat atggattgtt
      181 ccagaaaaac atgattccta taccggcaag aaatactcca agaaactgac tgcctgtgtc
      241 gcccatatac atttttgaag gatgccagtt aaaatataaa aagcctagca gtcctgctga
      301 aagtccgatg attgaagcaa gataaaggtt attaatatca ttactcattg tgatgactac
      361 aactgcagaa attattacac ccaaactgac agttgaagta ataccatcca tattatcgag
      421 catatttaca gaattcatga ttccaaccac ccagaatatt gttaaaagat aattgagaat
      481 catgttatcg aaaaggtcaa tataggtgcc ggtaaaaata aaactaaatg cgcatgatac
      541 ctgagcaaga aatttaagta caggttttgt gttataagca tcgtcagcaa ggccaatcag
      601 gaacccaatt gccattgcca tgataaaccc cataaactga gagttgtata atgcatcatt
      661 attattaaaa aaaacagagt atgttgccaa cgaaagcaag aatacaatat agaatgacaa
      721 tccacctacg gcaggcttgg acatggagcc ccatcggatt acagttccgt catttgtatt
      781 tcgaattcct aaagtaaaaa agaaacgtaa aaacaatgag ttaattaaaa atgaaaatgc
      841 cagtgatcct aaaaacaata cggcataaat aaataaatga tagttgtggt ttacaatgtc
      901 tttcattttg tttgaattaa accaaccatt gatgctacgc ctgcatgaga tgtgttggtg
      961 gggttattgt tatacttaat tagagcaaaa gtatagtata gaaatttaaa acaaaatagt
     1021 tgcatcacta aaatttggta aaattttatc tttttctgat aaaaccggct gatttacacc
     1081 ccaattaata tttaatgcag ggtcattata aattattcct gcttcagaat ctttattata
     1141 tggcttcgtg catttataat aaaaaagggt gttttctgct aaagaaacaa aaccatgcgc
     1201 aaaacccggt ggaatccaca ccatcaaatg atcttctgaa ttcaattcta ttgtaaaatt
     1261 ctttccataa gtagcagaag attttcgaat atcaactaca acatctaaca cagaaccgga
     1321 aataactttt atcagtttac cctgatcaaa tggagggttt tgaaaatgca ggccgcgaag
     1381 tacatttttg gctgacaatg aaaagttgtc ctgataaaat tctgcttcta taccaaggtt
     1441 ttttaatttc tctttatgat atgattccaa gaaatatccc cgctcatctg aaaaaacatc
     1501 agcctgcaaa atacaaatgc cgtttaaagc agttttaata attttcattg gaggttattt
     1561 cctaataaaa aaactaataa ttctgttcca gaagcctgtt ggctttttat catcttcgga
     1621 ataataacca tagccgtagc cataactgta tccgtagcca taaccatagc catagccgta
     1681 accataacca tatccatact tgagtttggt ttgatcaacg ctatttaaaa ctacagaaag
     1741 tcgtggtact ttattttcag aaataatttt attgatgtta tgaataaaca ttttacgact
     1801 gtaatttgca cgaagaatat atattggaaa atctgccttc tgaatcatag gaattccatc
     1861 actaacaaca ccaacaggag gtgtatcggc aataattact tcataccgta ctttcaaata
     1921 ttcaagcaat tcatccattt tagggctaat aatcaactca gatggatttg gtggaatagg
     1981 tcccgcagtg ataaaatcaa gattttcgag tgcactgtga ttaatacagt tttcaatggt
     2041 gtccttacca attagtatag tactcattcc tctgttgttt tccacattaa atcccaggtg
     2101 gattttaggc ttacgcatat ccaagtcgag aatgattact ttctttccgg aaaatgaaat
     2161 aacacctgcg aggttaattg cattaaatgt ttttccctca cctgaaatgg ttgatgtcag
     2221 cgccattaat tgcggcttat catcattatt gataaactga agattcgttc gaatagaacg
     2281 aaaagcttct gctatcacag attttggact tttgtcaacc aataattgcg agataggaat
     2341 ttctctttta tatcttggta caattcctaa taatggcgca tcggtatatt gcgcaatttc
     2401 ttcaagagac ccaatttgat tataaaacag atatttaata aatatcaata aaaaacttgc
     2461 aacaaaccca accataatac atgaactaag tatcaggctt ttattaggat aatatggtgc
     2521 aggtgcagga cgtgccgctt caagaataat atttttagaa acatatccgg cacgagttat
     2581 tgaaaactct gccttctttt caagtaaaag tgaatagaac ttttcattta cattgaaaat
     2641 tcgctgtaac cgttcaaatt ctgtttgttg ctcaggaata cccaaataat tactgttcag
     2701 tttcctatat tgcgattcca gttctgcttt tttctgctta atgccatttt cctcataagc
     2761 tacagactgc agtaaataat ctttctgtct ttgaattctt ccggaaagca tttgaacata
     2821 ttctgcttta tcagtaacca ttatcaaagc ttgttcgcgt ttatcaagta aaatatttaa
     2881 attacttagc acgtccttta tctcatcggt tttataaatt ccggttaaac tgagcatttt
     2941 ttcatcaact ttcttctcac tctctatcgt ttttttaagt tcagatatag caagtagatt
     3001 taaagtataa tctgttaatc tgagctccag ctgactcatc ttatcactta agtcagatgc
     3061 aagcaattcc ggagttatga ttctgttatt acttttaaat cgttcgagat tcatctctga
     3121 ctcggagaga tcattattta ctctgtaaag tgtagtatca ataaattcaa gaatacggtc
     3181 tgtagcttct ctgcttctca atacatcaaa cttcaaaaac tcttggctga tggcattgga
     3241 catatcagct gccttagtag catttttttc tttgattttg atatttattg tcttggcatc
     3301 tggattagcc atgatgactg aaagcttaga actgaaatcc ttaataagtg ccttatcgga
     3361 attaataata aacttaaaag tgttcttttt tagttgtgat tgatatttta atattcgctg
     3421 aaaatcaata atatttattt gaagtgaaat tccattcaga tgaatccatt tattaatttc
     3481 atattcttca tcaacttggt ctctttgccc aacaggtgta taagacaaaa caaatgtctt
     3541 ctcattgtta aactgaaggt aaaatggccg gtccatccat aaagagtctt ttaccaaaaa
     3601 gtttacttca aatggacacg atttatacat ttcgttttca agcaatgtgc ctttggaaat
     3661 gtaggttacc ctcataggca atgtggctat tgctttacca acaattatac ttgaacgaat
     3721 gagttcaagg ttaccggcta cttcattgtt ttgttcctga taaacatcgc gcgtaatatt
     3781 taatgccttg gtaacattgt tatcggtatt taacttcatc aatgctgccg attcatacat
     3841 tggcggtgta taacgaacta tgacatacca aacaattatg gaaagtgtca taaacattaa
     3901 gaaccactcc acattgcgct gagcaatggt tacaaaaagc ttaaaatcaa attcctcgtt
     3961 tagaggggtt atttgtt
//
LOCUS       DAOOOI010000145         3940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4084_length_3940_cov_25.037066, whole genome shotgun sequence.
ACCESSION   DAOOOI010000145 DAOOOI010000000
VERSION     DAOOOI010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4084_length_3940_cov_25.037066"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2447
                     /locus_tag="PKX59_10895"
     CDS             <1..2447
                     /locus_tag="PKX59_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91406.1"
                     /translation="MGGLKGETLPTVARNMKFRFTVRDNRAGGGGVVSSGDGGCQSST
                     SFVVTTSGSTPFTVTTPNGGESYSGGSTQTITWNNAGTNAVPFNVANVKISLSTDGGL
                     TYPTVITNSTANDGSEALTIPSVPATTTARIKIEAVGSIFFDISNANFSITVPVNGYA
                     FGTATTGSISCASAGPINSTLPVNLLGSYTGPVSLTYVSGAPGGTNVTISPNSFTATG
                     NATVSLNNTGALTPGTYTVVVQGSGPAPGGTQTANVSFTVNAGAGPSISVHPASQTTC
                     VGTGVNFSVTASGALSYQWEYSTNGGTTWSIIAGAISSSYNFPSPTVTHNNWQFRCVV
                     TGQCGVSNSNAAVLTVNTAPAVTGQPANLAVCSGSAASFTVTATGSSIGYQWELSTDG
                     GTTWNPIGGATSATYSIASTLIGQNGHRFRCVVTGVCPPAATSSSATLTVNSSVTVTT
                     NPSNQTICEGTGTSFSAAASGSGLNYQWEVSTDGGVTWNNVSNGANYSGAGTPTLTLI
                     NTPPTFNQNRYRCNISSPPCTPGITASALLTVNSYPVITAQPVSQTFCEGSNVTLSVS
                     ATTSVGALTYQWQASTNGGATWFNLVFPTSSLTPTNNSTLVQTNVPAIQNGYRYRCAV
                     TAGCGTTFTNAAIFTVNTYPVISLNALPKTVCVSDAAFNLSASYSGGVWSGTGVTSGG
                     LFTPSTAGIGNHTVTYTATNAGCTKAASSLVFVQECADRHRKLNEKHAVLIMPNPNKG
                     NFSLKLNTDLYTSLGVKVYGADGRLYRTYSYAGLTYGSVIPMDLTSLAQGTYYLYMYN
                     SENGFISRGDGVIIVH"
     gene            2621..>3940
                     /locus_tag="PKX59_10900"
     CDS             2621..>3940
                     /locus_tag="PKX59_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ91407.1"
                     /translation="MIGVRDLKLAYGKRVLFEEVNLNFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNKGSVEITPGERMAVLKQNQFQFDEETVLNTVMMGHTKLWQVAKERESI
                     YALADFTEEDGIRAGELEGEFGEMGGYTADSDAATLISELGVTEAYHQSLMKDIPATL
                     KVRVLLAQALFGNPDILLLDEPTNGLDIETISWLENFLADYENIVLVVSHDRHFLDAV
                     CTHVADVDRQKIKIFTGNYTFWYESSQLMARQVNDKNKKTEDKRQALIDFIARFSANA
                     SKSKQATSRKKALEKLTIEEIEPSYRKYPGIIFQQLREVGNQILNVEKLKKTVDGRVL
                     FADVTFTINKGDKIALLSRDPLAVTSFFNIITAEAMADSGSYEWGSTVTHAYLPQENA
                     TFFGQDVNLMDWLRQYVPDHVKDVDEPFLRGFLGKMLFSGDDVMKKTN"
BASE COUNT         1150 a          798 c          835 g         1157 t
ORIGIN      
        1 caatgggcgg tttaaaaggt gaaactttgc caactgttgc cagaaatatg aagtttagat
       61 ttacggttag ggataacaga gcaggtggtg gtggtgttgt ttcaagtggt gatggtggtt
      121 gtcaatcttc aacctcattt gttgttacta cttcaggatc tacccctttt acagttacca
      181 ctcctaatgg cggagagtcc tattctggag gttcgacaca aactattact tggaataatg
      241 ccggaacaaa tgcagttcca tttaatgttg ccaatgtaaa aatttcttta tcaactgatg
      301 gaggacttac atatccaacc gtaattacaa atagtactgc taatgatggt tcagaagcat
      361 tgactattcc ttctgttcct gcaacaacca cagcaagaat taaaattgag gctgttggta
      421 gtatcttctt tgatatctct aatgccaatt tttcaattac agttcctgtt aatggctatg
      481 cttttggtac agcaacaaca ggatctattt cttgtgcatc agccggtcct attaattcaa
      541 cactgcctgt taatttactt ggttcatata caggtcccgt gtcattaact tatgtttcag
      601 gtgctcccgg tggtacaaat gttactattt ctccaaattc atttaccgca actggaaatg
      661 caactgtttc tttaaacaat acaggtgctt taactcctgg aacttacaca gtagtggtac
      721 agggtagtgg ccctgctcca ggcggtacac aaacggctaa cgttagcttt accgtaaacg
      781 caggtgcagg tccttctatt tctgtacatc ctgcaagcca aacaacttgt gtgggaacag
      841 gggttaactt tagcgttaca gcaagtggtg ctctttctta tcagtgggag tacagtacca
      901 atggtggtac tacatggagt attattgctg gtgctattag tagtagttat aatttcccaa
      961 gcccaacagt tactcataac aactggcaat tccgttgtgt agttaccggc cagtgcggcg
     1021 tttcaaattc taatgctgca gtgttaactg taaatacagc tcctgcagta accggtcagc
     1081 ctgcaaacct tgcagtatgc tctggtagcg cagcttcttt cacggttaca gctactggtt
     1141 cttctatagg ttatcaatgg gaattaagta ctgatggtgg taccacctgg aatccaattg
     1201 gcggtgctac cagtgctact tactcaattg catcaacctt aattggtcaa aacggtcaca
     1261 gattccgttg tgtggtaaca ggtgtttgtc ctcctgcagc tacttcaagt tctgctacac
     1321 ttacagttaa ttcatctgta actgtaacca ccaatccttc taaccaaacc atttgcgaag
     1381 gcacaggtac tagctttagt gcagcagcaa gcggcagcgg tcttaactat caatgggaag
     1441 taagtacaga tggtggcgtt acctggaata atgtttccaa cggcgctaac tattctggtg
     1501 caggtacacc tacacttaca ttaattaata cgcctcctac cttcaatcaa aacaggtaca
     1561 ggtgtaatat ttcaagccct ccttgtacac cgggcattac tgcttctgct ttacttacag
     1621 taaactctta tcctgtaatt actgcacagc ctgtaagcca gaccttctgc gaaggaagta
     1681 atgtaacatt atctgtaagc gcaactactt cagttggtgc acttacttat caatggcagg
     1741 caagtaccaa tggtggtgct acctggttca acttggtatt ccctacttca tcgcttacac
     1801 ctactaataa cagtacatta gtacagacaa atgttcctgc tattcaaaac ggttacagat
     1861 atcgttgcgc agttactgca ggttgcggta ctacgtttac caatgcagca atatttacgg
     1921 tgaatactta ccctgttatt tctttaaatg ctttaccaaa aacagtttgt gtatcagatg
     1981 cagcattcaa tctttctgca tcttatagtg gtggcgtttg gtcaggcaca ggtgttacat
     2041 caggtggatt atttactcca tctacagctg gtataggcaa ccatactgtt acttatactg
     2101 caaccaatgc cggttgtaca aaagcagcca gctcattggt atttgtacaa gagtgtgccg
     2161 acagacacag aaaactcaat gagaaacatg ctgtattgat aatgcctaat ccaaacaaag
     2221 gtaatttcag tctgaagttg aacactgacc tttatacttc tttaggagta aaagtgtatg
     2281 gtgctgatgg cagactgtac agaacttata gctacgctgg cttgacttat ggtagtgtta
     2341 tcccaatgga tctcacttca ttggctcagg gtacttatta tctgtatatg tacaatagcg
     2401 aaaacggctt tatcagtcgt ggagatggtg ttattatcgt acattaatag aaattaattt
     2461 cttaaaacat aaaaaccgtt ctgcaagcaa tgcagagcgg ttttttttgc ataatacctt
     2521 cacccaaata accattcaac taattaacta atcaactacc cattcacctt ctcccttccc
     2581 acctttctcc ttacctttgc accctcaaaa aaaatcaatc atgatcggtg ttagagattt
     2641 gaaacttgcc tatggcaaaa gggttctttt tgaagaagta aacctcaact ttaccaaagg
     2701 gaattgttat ggcattatag gagccaatgg cgctggaaaa agtacttttt taaaaatact
     2761 aagtggcgaa atagaaccaa acaaaggcag tgtggaaata acgcccggcg agcggatggc
     2821 tgttttgaaa caaaaccagt ttcagtttga cgaagaaacc gttttaaata cagtgatgat
     2881 gggtcacacc aaactctggc aggtggcgaa ggagagagaa agcatttatg cgcttgccga
     2941 ttttactgaa gaagatggaa taagagccgg agaactggaa ggtgagtttg gtgaaatggg
     3001 cggttatact gcggatagtg atgctgctac gctaataagc gaattgggtg ttacggaagc
     3061 ttatcatcaa tcgttgatga aagatattcc tgccacttta aaagtcaggg tgctgctggc
     3121 acaggcatta tttggcaatc ccgatatttt attgcttgat gagcctacca atggattgga
     3181 tatagaaacc atcagttggt tagaaaattt tctggccgat tacgaaaata ttgtattggt
     3241 agtcagccac gaccgtcact ttctggatgc tgtgtgtacc catgtcgccg atgtggacag
     3301 acagaaaatt aaaatattta ccggtaacta tacattctgg tacgagtcat cgcaattgat
     3361 ggcacgccag gtaaatgata agaataaaaa gaccgaagac aaaagacagg ctttgatcga
     3421 cttcattgca cgctttagtg ccaatgccag taagagtaaa caggctacat caagaaagaa
     3481 agcattggaa aaactgacca ttgaagaaat agaaccaagc taccggaaat atcccggtat
     3541 catttttcag cagttgagag aagtgggcaa tcagattctc aatgtagaga aattgaaaaa
     3601 aacggtggac ggtcgtgtat tgtttgccga tgttacattt acaataaaca aaggagataa
     3661 aattgcattg ttgagccgcg acccgctggc ggttaccagt ttcttcaata tcattacagc
     3721 agaagccatg gccgacagtg gttcttacga atggggaagt accgttacgc atgcatatct
     3781 gccacaagag aatgcgactt tttttggtca agacgttaac ttgatggact ggctgcgcca
     3841 atacgtaccc gatcatgtaa aagatgtgga tgaaccattt ttacgtggtt ttcttggcaa
     3901 aatgcttttt agtggtgatg atgtaatgaa aaaaaccaac
//
LOCUS       DAOOOI010000146         3937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4087_length_3937_cov_21.142452, whole genome shotgun sequence.
ACCESSION   DAOOOI010000146 DAOOOI010000000
VERSION     DAOOOI010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4087_length_3937_cov_21.142452"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2589
                     /locus_tag="PKX59_10905"
     CDS             <1..2589
                     /locus_tag="PKX59_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91408.1"
                     /translation="SPGTIRRTQIAAYSGVCGSGMALVACNLNAPGCGVAVPNTPTTM
                     SELSLAGLIPGNTYYVVVDGENYSTGTFSIIAIDGAGTMPDIEGQECSTPIIVCNPNT
                     QVGDPGFQLFGNRCDFPGAGTNCLTRAERGSAWYQINIASAGNLEFNLIPNDWVGAPS
                     TACTDYDFAVWKIAGTGATTCAGIAAGATPVKCNYSPLGITGLYSGSNGTAPPAYPGF
                     GGAYQSRLSVAAGEVYILVVSNYSNSISGFTLNFPASAPVDYTAGGTTAYWTGAESND
                     WFTAANWGGCNIPSCTVDAVIPLSSANQPYIGTAGATCKDLKLEPGGTLIMDNNQFLS
                     ICGNVQNNGELNIATGSGFLFGNGSVNQTLNGNLVSPNRFSHITVNKTGGKVDLLQNI
                     EMQGSLALIGPSSRFDAINKTVTLKGSLSNSAGQFNMGNNGNLVLTGTSTQNISNNDT
                     LTNVIVNKSGNTVVLIRDMKIGPTGSLSLIASKIQTTSANEVYVMNDAASAVSAGSSS
                     SYIEGNLRRNVPTIVSPRVYDFPVGNATKGYQRLNLNLYNGISPAVGSIQASFSDYLT
                     DIWGLGLDAPCLMNYNDTMLNNGYWTLTPANTANGEANITLYNRTYTNAKTNYGVGIN
                     PNGAGWMIPPVISGGCVTPPVTAVLRNGVGLSFTSGQSILVGTAQGKVSPLPVELLSF
                     EATAGKQSIFLTWSTASEKNNAGFYLERSTDGNNFDNITWVDGHGTTNQVNNYSYEDK
                     NVKEGVLYYYRLKQIDYDGKFEYSFVKTAMIGDLKNVILSAAPNPYRGSTKINYSIPT
                     DGSVVILITDMTGRILKNIDAGMQTKGSYSIPFSAIADGHPSGVYNVTLFFNDKPYRL
                     KISEYR"
     gene            complement(2595..>3937)
                     /locus_tag="PKX59_10910"
     CDS             complement(2595..>3937)
                     /locus_tag="PKX59_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91409.1"
                     /translation="VLLKNRGNLAMPVNVSFYNKEKLIASQWTGVFDGKFKLSSSVSG
                     ADKIVLDTYDQSLDLNPFNNSMKTKGVFRKWKPLQLRFLTMLENPERTQLFYLPAVAY
                     NNYNGWMAGVILHNYSLLSKKFEFGIVPMYAFKSKSVAGVATASYQFNIYNGWFDRII
                     AKISPTLFDEGLSENRSIFKGYFSVPASLSFRIRKSQYKPYSEKYVNIIHHYINQTFS
                     TQNNSSATNEINITQLEFASFNYSKTDPGSLSFILEKGGHYAKLTGTWKKEITLTRRK
                     KDLSLRFFAGTFLDNSDYGGEANFRSSAWRGTDDYSYSGLWFGRNEYDGFWAHQMQRA
                     DGAMAAYYPVRTDHWLAALNVSVKTPVSGLRLFADGITFYNANKVSPGATVVRYDAGI
                     MLSIVHDAFEIYWPLIVSKEVRDYYDLNDIKLADRIRFVFNINKLNPVLLRKMR"
BASE COUNT         1268 a          721 c          752 g         1196 t
ORIGIN      
        1 agccccggaa ctataagaag aacacaaatt gctgcttaca gtggtgtttg tggttcaggt
       61 atggcattag tagcgtgtaa ccttaacgca cctggatgtg gcgtagctgt tccaaatacc
      121 cctacaacaa tgtctgaatt atcgttagca ggattaattc ccggaaatac ttattatgta
      181 gtagttgatg gcgaaaatta ttctacaggc acattcagta ttattgctat tgacggtgca
      241 ggcacaatgc ctgacataga aggtcaggaa tgtagtactc caattattgt ttgtaatcca
      301 aatactcagg ttggcgaccc gggatttcag ttattcggga accgatgtga tttccctggt
      361 gcaggaacca actgtcttac acgagcagaa cgtggctctg cttggtatca gattaacatt
      421 gcttcagcag gaaatcttga atttaactta attccgaatg actgggtggg tgcgccaagt
      481 acagcatgta cagattatga ctttgctgta tggaaaattg caggtaccgg tgctacaaca
      541 tgtgcaggaa ttgctgctgg tgctacacct gtaaaatgta attatagtcc tcttggaatt
      601 actggtttat acagtggttc caatggaact gcaccaccgg cttaccccgg ttttggggga
      661 gcatatcaat caagacttag tgtagctgca ggtgaagtat atattttagt tgtaagtaat
      721 tattcgaata gtatttcagg gtttacctta aatttccctg cttctgctcc tgtagattat
      781 actgcagggg gaacaacagc ctattggact ggagcagaaa gcaatgattg gtttactgct
      841 gctaactggg gtggatgtaa tattccaagt tgtaccgtag atgctgtcat accactttca
      901 tctgcaaatc aaccttatat tggaacagca ggtgccactt gtaaagacct taaacttgaa
      961 ccgggtggaa ctctaataat ggacaacaat cagttcctct ctatttgtgg aaatgtgcag
     1021 aataatggag aattaaatat tgcaacagga tcagggtttc ttttcggaaa tggtagcgta
     1081 aatcaaactt taaatggaaa cttggtttct ccaaacagat tctcgcatat cactgtaaac
     1141 aaaacaggtg gaaaagttga cttacttcaa aatatagaaa tgcagggaag tttggcatta
     1201 attggtccta gctcaagatt tgatgccatc aacaagactg ttacattgaa aggaagctta
     1261 tcaaattctg ccggtcagtt taatatggga aataatggaa acttagtatt aaccggtact
     1321 agtacacaaa acatcagcaa caatgatacg ctgaccaatg tgattgtaaa caaaagcgga
     1381 aacactgtag ttcttatccg tgatatgaag ataggaccta ccggaagttt aagtctaata
     1441 gcatcaaaaa tacaaaccac ttctgcaaac gaagtttatg taatgaatga tgctgcatct
     1501 gcagttagtg caggttcgag tagttcctat attgaaggta atctcagacg aaatgttcct
     1561 acaattgttt cgccaagagt ttatgacttc cctgttggaa atgcaacaaa aggatatcaa
     1621 cgtttaaact tgaatttata taatggaata agtccggcag taggaagtat tcaagcctca
     1681 ttctccgact atttaacaga catttgggga ctaggattag atgcaccttg tctaatgaac
     1741 tacaatgaca ccatgttaaa caatggctat tggacattaa cacctgcaaa tacagcaaat
     1801 ggcgaagcta atattacctt atacaatcgt acatatacaa atgccaaaac aaattatggt
     1861 gttggtatca atccaaatgg tgcaggatgg atgattccac ctgtaattag tggcggatgc
     1921 gtaactcctc ctgtaacagc tgtattaaga aatggtgtag ggttgagttt cactagcgga
     1981 caaagcatat tagtgggtac agcacaaggt aaggtatcgc cattacctgt agaattactt
     2041 tcattcgaag caacagctgg taaacaaagt attttcctga cttggtcaac agctagtgag
     2101 aaaaataatg caggattcta tttggagaga agtactgatg gcaacaactt cgacaatatc
     2161 acctgggttg atggacatgg aactaccaat caggtaaaca attatagtta cgaagataaa
     2221 aatgttaaag agggtgttct atactactac cgattgaagc agattgatta tgacggtaag
     2281 tttgaatata gttttgtaaa aactgctatg attggtgact tgaaaaatgt tatactgagt
     2341 gctgcaccta atccatatcg tggtagcaca aaaattaatt actcaattcc aactgatggc
     2401 agtgttgtaa ttctaattac tgatatgaca ggtagaatat tgaaaaatat tgatgcagga
     2461 atgcaaacta aaggcagcta cagcattccg ttcagtgcta ttgctgatgg acatccttcg
     2521 ggagtttata atgtaacact attcttcaat gataaaccgt atagattgaa aatttcggaa
     2581 tacagataaa aatctcagcg cattttgcgt agcaagacag gattcagttt gttaatatta
     2641 aaaacaaacc ggatcctgtc tgctagtttt atatcattca agtcatagta atctcttact
     2701 tcctttgaaa caatcagtgg ccaataaatc tcaaatgcat catgaactat acttaacatg
     2761 ataccggcat cgtaacgaac taccgttgca ccaggactta ctttgtttgc attataaaat
     2821 gtaattccat cagcaaataa tctcaagcct gataccggtg ttttaactga aacatttaat
     2881 gcagcaagcc aatgatctgt acggacagga taatatgctg ccattgcacc atctgctctt
     2941 tgcatttgat gcgcccagaa tccatcgtat tcattcctgc caaaccacaa acctgaataa
     3001 gaataatcat cagtaccgcg ccatgcactt gaacggaaat tagcttctcc tccataatca
     3061 gaattatcta aaaatgttcc tgcaaaaaaa cgcaatgaca aatctttttt tctccgcgtt
     3121 aatgttattt cctttttcca tgttcctgtt aacttagcat agtgacctcc tttttcgaga
     3181 ataaatgaca atgaaccggg gtctgtttta ctgtaattaa aacttgcaaa ctcaagttgt
     3241 gtaatattga tttcattggt tgctgacgaa ttattttgtg tactgaatgt ttggtttata
     3301 taatgatgaa taatgtttac gtacttttct gaatatggct tatactgtga cttacggatt
     3361 ctaaaactca atgatgcagg aacagaaaag tatcctttaa agatacttct attttcagat
     3421 aacccctcat caaatagtgt aggtgatatc ttagcaatta ttctgtcaaa ccaaccattg
     3481 taaatattaa actgataact tgcagttgca acacctgcaa cagattttga tttgaatgca
     3541 tacattggta caattccaaa ttcaaatttt ttactcaaca atgaatagtt atgcagaata
     3601 acgcctgcca tccatccgtt gtaattatta tatgcaacag caggcagata aaacaactgt
     3661 gtcctttcag gattttcgag cattgttaaa aaacgaagtt gtaatggttt ccattttcta
     3721 aacacacctt ttgttttcat tgaattatta aatggattta aatccaaact ttgatcataa
     3781 gtatctaaca caattttatc tgcacctgaa acagaacttg acaacttgaa tttgccgtca
     3841 aaaactcctg tccattgcga ggcaattaac ttttctttat tgtaaaaaga cacattaaca
     3901 ggcatagcaa gatttcctct attctttagc aatacat
//
LOCUS       DAOOOI010000147         3919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4118_length_3919_cov_9.232919, whole genome shotgun sequence.
ACCESSION   DAOOOI010000147 DAOOOI010000000
VERSION     DAOOOI010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4118_length_3919_cov_9.232919"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1783)
                     /gene="hutU"
                     /locus_tag="PKX59_10915"
     CDS             complement(107..1783)
                     /gene="hutU"
                     /locus_tag="PKX59_10915"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOZ91410.1"
                     /translation="MTVISDKLDISKLKTPTGNTISCKGWVQEAALRMLHNNLDPDVA
                     ERPEDLIVYGGTGKAARNVESFHLIVKALRDLNEDETLLVQSGKPVGILPTHKDAPRV
                     IIANSNLVGHWANWDYFRELEAKGLIMYGQMTAGSWIYIGSQGIVQGTYETFGEVARQ
                     HFGGSLKNTLNVTAGLGGMGGAQPLAITMNEGTCLAAEMEEWRVDKRIQTRYLDEKYS
                     DLDKAIDRAVEATKKGEAISIAYIGNAIDMLARLIERNIIPDTLTDQTSAHDALVGYF
                     PEGLNVTDAKVLRDSNPEEYIKRSKQSMRKHVELMIALQKKGAVTFDYGNNIRQQALE
                     MGLKNAFDFPGFVPAYIRPLFCEGKGPFRWAALSGDPNDIYETDKKILELFPENQGLH
                     RWITMAQKRIAFQGLPARICWLGQGEREKAGLAFNELVKSGKVKAPIVIGRDHLDCGS
                     VASPNRETEAMKDGSDAIADWPILNALINTAGGASWVSLHHGGGVGIGYSIHAGMVIV
                     ADGTADAATRLKRVLHNDPAMGVLRHLDAGYDIAADTARKHRLNLRDRLA"
     gene            complement(1900..>3919)
                     /gene="uvrB"
                     /locus_tag="PKX59_10920"
     CDS             complement(1900..>3919)
                     /gene="uvrB"
                     /locus_tag="PKX59_10920"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOZ91411.1"
                     /translation="FRINSPFKQTGDQPQAISELVKGLDRGDRHQTLLGVTGSGKTFT
                     VANVIEKVQRPTLILSHNKTLAAQLYGEFKQFFPDNAVEYFVSYYDYYQPEAFIPTTN
                     TYIEKDLAINDEIEKLRLSTTSTLLSGRKDIIVVSSVSCIYGLGNPADFLKGIIRISK
                     GERISRNTFLHALVTALYSRTEGEFKRGTFRVKGDTVDLFLAYAERALRFTFFGDEIE
                     EIESIEPATGKRIELLDHVAIFPATIFVTNAKQQLKAIWQIQEDMVKQVDYFKENGRN
                     LEAKRLDDRVTHDLEMIRELGYCSGIENYSRYFDGREPGMRPFCLLDYFPEDYIMVID
                     ESHVTIPQIRAMYGGDRSRKVNLVEYGFRLPAAMDNRPLKFEEFETLQQQVVYVSATP
                     ADYEMTKSEGVIVEQLIRPTGLLDPLIEVKPCGNQVDDLLEEIDVTVKKNERVLVTTL
                     TKRMAEELAKYLTKLSIRCRYIHSEVETLDRVELLRDFRLGLYDVLIGINLLREGLDL
                     PEVSLVAIMDADKEGFLRSNRSLTQTAGRAARNVNGRVIMYADKITESMRKTIDETNR
                     RREKQMKYNMENDIVPTPLNKSREMILQQTVVSGTKNVNAYIESENVTVAADPVVQYM
                     TEEQLGSAITKTKKQMEVAAKEMDFMEAARLRDEMFELQKLLKERFGK"
BASE COUNT         1096 a          904 c          674 g         1245 t
ORIGIN      
        1 ccggtaaatt tatctctgtc atcgcctgtg ctatcatcat cgggtgtaca ggctgtgaat
       61 gccaacatca ccaacgttaa taaaaataat acttgcttat tttttctcat gccaatctat
      121 cacgcaaatt taaacgatgt tttcttgctg tatctgcagc aatgtcataa cctgcatcaa
      181 gatggcgcag aactcccata gcagggtcat tgtgcaacac acgttttaat cttgttgctg
      241 catcagcagt accatcagcg acaattacca tacctgcatg tatgctgtag ccgataccta
      301 ctccaccacc atgatgtaaa ctaacccaac ttgcaccacc ggcagtgttg atcaatgcat
      361 ttagtattgg ccagtctgca atggcatcgc taccatcttt cattgcttct gtttcgcggt
      421 ttggcgatgc aacacttcca caatccaaat ggtcgcgacc aataactata ggtgccttta
      481 cttttcctga tttaaccaat tcgttaaaag caagacctgc tttttcgcgt tcaccttgtc
      541 caagccaaca gatgcgtgcc ggcaatccct gaaatgcaat gcgcttttgt gccatagtta
      601 tccaacggtg taagccttgg ttttcaggaa acaattccag aattttttta tcggtctcat
      661 aaatatcatt agggtcgcca ctgagtgcag cccaacgaaa cggacctttg ccttcgcaaa
      721 ataacggacg tatgtatgca ggaacaaatc ccggaaaatc aaaagcattt tttaatccca
      781 tctccaatgc ctgctggcga atgttgtttc cataatcaaa cgtaacagca ccttttttct
      841 gcaaggcaat catcaattct acatgcttgc gcatgctttg tttagaacgc tttatgtatt
      901 cttcaggatt agaatcacgt aaaacttttg catctgtcac atttaatcct tcagggaaat
      961 atcctaccaa agcatcatgt gcagatgtct gatctgttaa ggtatcggga ataatatttc
     1021 tttcaatcaa tcgtgccagc atatcaatag cattgccaat ataagcaatt gaaattgctt
     1081 cacccttttt tgttgcttca acggcacggt caatggcttt atccaaatca gaatattttt
     1141 cgtcaagata acgcgtttgt attcttttgt caacacgcca ctcttccatt tctgctgcaa
     1201 gacatgtgcc ttcattcatt gttattgcca atggctgtgc accacccatt ccaccaagac
     1261 ctgctgttac attcaaggtg ttttttagcg acccaccaaa atgctgacgt gccacttcac
     1321 caaatgtttc ataagtaccc tgaacaattc cttgcgagcc gatgtaaatc caactgcctg
     1381 ctgtcatctg tccgtacatt atcaaccctt ttgcttccaa ctcgcggaaa taatcccagt
     1441 tagcccaatg gccaacaagg ttggagttgg caataattac tcttggagca tctttatgcg
     1501 ttggtaaaat tccaaccggt ttacctgatt gcactaacaa tgtttcgtct tcatttaaat
     1561 cacgcagggc ttttacaatg agatgaaatg actctacatt tcgtgcagct tttccggtgc
     1621 cgccataaac aattaaatct tcaggacgtt ctgccacatc aggatcaagg ttgttgtgca
     1681 acatgcgcaa tgcagcttcc tgaacccaac ctttacaact aatagtattt cctgtaggtg
     1741 ttttaagttt actgatgtct aatttatctg aaattactgt catgattttt aaactttaag
     1801 tttcgcaaag ataaaagtta caaggcattt ttaaaatatc aatttaggtg agaaggccat
     1861 gatgaaaaac tcctgaataa tctgtcaaat gtgaaagtgc tatttgccaa aacgctcttt
     1921 caaaagtttt tgtagctcaa acatttcatc acgtaaacgt gctgcttcca taaaatccat
     1981 ctcttttgct gcaacttcca tttgtttctt tgttttggtg atggcacttc ccaactgctc
     2041 ttctgtcata tactgaacaa cagggtcggc agcaactgtt acattttcac tttcaatata
     2101 agcatttaca tttttagttc cggaaacaac tgtctgttgc aaaatcatct cgcgcgattt
     2161 gtttaatggt gtaggtacaa tatcgttttc catattgtac ttcatttgtt tttcgcgtct
     2221 gcggttggtt tcgtcaatgg tctttctcat gctctcagta attttatcgg catacataat
     2281 tactcttccg ttaacattac gtgcagcacg tcctgctgtt tgtgtcaatg acctattact
     2341 tcgtaaaaat ccttctttat cagcatccat aatagccacc aaagaaactt caggcaaatc
     2401 gagaccttcg cgcagcaagt ttattccaat taaaacatca tacaatccaa gacgaaaatc
     2461 gcgtaacaat tctactctat caagtgtttc tacttcactg tgaatatagc ggcaacggat
     2521 ggaaagcttg gtcaaatatt tagccaactc ctctgccatc cgttttgtca atgtagtcac
     2581 taacacacgc tcattcttct ttactgttac atctatttct tctaataaat catctacctg
     2641 attgccgcaa ggttttactt caatcaaagg atcaagcaat cctgttgggc gaatcaattg
     2701 ctctacaatt acaccttcgg attttgtcat ttcatagtcg gcaggtgttg cactaacata
     2761 aacaacctgt tgttgaagtg tttcaaactc ttcaaacttc agaggtctgt tgtccattgc
     2821 agcaggaagt ctgaaaccgt attcaaccag attaactttc cttgatcggt cgcctccata
     2881 catggctctt atctgaggaa tggttacatg gctttcgtca atgaccataa tataatcttc
     2941 aggaaaatag tcaagcaaac agaaagggcg catgccgggt tcacgaccat caaaatagcg
     3001 tgaataattt tcaatgcctg aacaataacc taattctcga atcatttcaa ggtcatgtgt
     3061 tactctgtca tcgaggcgct tggcttccag attgcgacca ttttctttaa aataatccac
     3121 ctgctttacc atatcctcct gaatctgcca gatggctttc agttgctgct ttgcattggt
     3181 aacaaaaata gttgccggaa aaattgccac atgatccaat aactcaattc gttttcctgt
     3241 tgcgggttca atactttcaa tctcttcaat ttcatcacca aaaaaagtaa atcgcaatgc
     3301 ccgttcagcg taggctaaga acaaatcaac agtatcacct tttacccgga aagtgccacg
     3361 cttaaattct ccttctgtcc gcgaatacaa tgcagttact aatgcatgta aaaaagtatt
     3421 acggctgatg cgctcacctt ttgaaattct gataataccc tttaaaaaat cagcaggatt
     3481 tcccaatccg taaatacaac ttacactgct tacaacaatg atatctttcc gtcctgatag
     3541 caaggttgaa gttgtactta aacgtagttt ttcaatctca tcattaatgg caaggtcttt
     3601 ttcaatgtag gtgttggtag taggaataaa tgcttcgggt tgatagtagt cgtagtacga
     3661 aacaaaatat tctacagcat tgtcaggaaa aaactgttta aactcaccat aaagctgcgc
     3721 tgccaatgtt ttattatgac ttaaaattaa ggtcggtcgc tgtactttct caattacatt
     3781 ggctacagta aatgtttttc cactgccggt tacccctaaa agtgtttgat gcctgtcgcc
     3841 tctgtccaac ccttttacca actcgcttat ggcctgaggc tgatcaccgg tttgcttaaa
     3901 aggtgagttg atgcggaaa
//
LOCUS       DAOOOI010000148         3897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4140_length_3897_cov_6.776939, whole genome shotgun sequence.
ACCESSION   DAOOOI010000148 DAOOOI010000000
VERSION     DAOOOI010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4140_length_3897_cov_6.776939"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2270)
                     /locus_tag="PKX59_10925"
     CDS             complement(<1..2270)
                     /locus_tag="PKX59_10925"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOZ91412.1"
                     /translation="MSFFKTALLSCWLLCNSILYAQPTYTQYVNPFIGTGGHGHTFPG
                     ATLPFGMVQLSPDTRIDGSWDGCSGYHYSDSVLYGFSHTHLSGTGVSDYGDILLLPMN
                     DEPALDESVPTATFSHNFENATPGYYSVKLLADSIDVELTSTLRTGMHRYTFNKPGLA
                     YIYLDLNHRDKLTASNINIVYDSTIKVYRKSEGWAKEQILYANIRFSKPFIQSKQLGN
                     KKAVFVFSVSEKESIMAKVALSAVDDDGAERNMILENPDWNFEHIKINAAQKWNEELS
                     KIKVSSSDKNKLTVFYTAMYHCMMHPNIYNDVDGRYRGRDLQIHQTDHNYYTVFSLWD
                     TFRTWHPLMTIIDSARTVDFIRTFLLQYEQGGLLPVWELAANETECMIGYHAVSVIAD
                     AMMKGIKGFDYEKAFEACKFSAEQRNRFGLGAYIDKGFLEAEDEHESVSKTLEYAYDD
                     WCIAQMAKLLGKEDDYNTYMQRSQSWKNLFDPVTGFIRPRSNGGWLNPFDPFEVNNNY
                     TEANGWQYNFFVPQDIDGMISYNGGKEKFEKKLDQLFSASTKTTGREQSDITGLIGQY
                     AHGNEPSHHIAYLYNYVDKPGKTEKIVNKIINDFYKNSPDGLIGNEDCGQMSAWYVMS
                     ALGMYPVCPGSNQYTLTTPQFDKVEILTSGNKTFTIDVKNKTNKANFLRVKKTKNISF
                     RDGLLFNHNRFSTDNLLVLTADSIPSILRKKDLADSIYYSDDLFRVPIITASTTVFKD
                     SLKVSIKSFQKDKNIV"
     gene            2603..>3897
                     /locus_tag="PKX59_10930"
     CDS             2603..>3897
                     /locus_tag="PKX59_10930"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91413.1"
                     /translation="MKRTIQSLIFIAVTLLTTNIFAQSWQLVGPVGFTSFNAKYTNIA
                     FNNNDEPYVVFSDGSVNAKASVMKFDGSNWVYVGTAGFSQGIADYTDIAFDNNGTPYV
                     AYSDHANNDNVTVMKFDGSSWVAVGNVGFSTGEPYYNSLAINSNGVPYVVFQDATSLS
                     KATVMKFDGNSWVVVGTAGFSSVSVKEPTIAIDNNNTPYVAFTQWGGLYNASVMKFDG
                     SNWVNVGPVGFTTNSADYASLAFSNSGEPYMAFIDGTQNSHVSVMKFNGSSWALVGNA
                     GFSTGTSHYPSLAFSNTGEPYVAFKSSFNNSQATVMKYDGSAWVAVGSTGFSSGWADY
                     TSLAFNSSGVPYVAFEDINNSYRATVMKFGNGTSSINSMAADNSIEVYPNPANDYLNL
                     TNLPVGANIKINDAIGKNVYDASITTKETSINTSAFTNG"
BASE COUNT         1216 a          695 c          712 g         1274 t
ORIGIN      
        1 taaactatgt ttttatcttt ttgaaaagat ttaatactga ctttaagaga atctttaaat
       61 accgttgttg aagcagtaat tataggaaca cgaaataaat catcggaata gtaaatacta
      121 tcagccaaat cttttttacg aagtatagaa ggaatactgt cggcagttag tactaaaaga
      181 ttgtcagtgc taaaacgatt gtgattaaaa agcaaaccat cccgaaaaga aatatttttt
      241 gttttcttca ctctaaggaa atttgctttg tttgttttgt tctttacatc aatagtaaat
      301 gttttatttc ctgaagttaa aatttcaact ttatcaaact gaggagtagt cagcgtatat
      361 tgattgcttc ccggacaaac agggtacatg ccaagagcac tcatcacata ccatgcactc
      421 atctgtccgc aatcttcatt gcctattaaa ccatccggac tgttcttgta aaaatcattg
      481 attattttat tgacaatttt ctcagtcttt cccggcttgt caacataatt gtacaagtaa
      541 gcaatgtgat gactaggttc gttaccgtgt gcatactgtc ctattaatcc tgtaatatca
      601 gattgttctc tacctgtagt ttttgtagaa gcactaaaaa gctgatctaa ttttttttcg
      661 aatttttctt ttccgccatt gtatgaaatc ataccatcaa tatcctgagg aacaaaaaag
      721 ttgtattgcc atccatttgc ttcagtataa ttgttgttca cttcaaaagg gtcaaaagga
      781 tttagccagc caccgttgct tcttggccgg ataaatcccg tcacagggtc aaataaattt
      841 ttccatgact gcgagcgttg catgtaggtg ttgtaatcat cttcttttcc caacagtttt
      901 gccatttgtg caatgcacca gtcatcataa gcatactcta atgtttttga aacagattca
      961 tgctcatctt ccgcttcaag aaaacctttg tcaatatatg cacctaatcc aaaacggttg
     1021 cgttgttctg cactaaactt gcatgcttca aatgcttttt catagtcaaa acctttaatg
     1081 cctttcatca ttgcatcggc aataacactc actgcatgat agccaatcat acattctgtt
     1141 tcattagctg ccagttccca aacaggaagt aagcctcctt gctcatactg caataaaaaa
     1201 gtacggataa aatcaactgt gcgtgcacta tcaattatag tcatcaacgg atgccatgtc
     1261 ctgaatgtat cccacaacga aaagacagta taataattat ggtcagtctg atgaatttgt
     1321 aaatctcttc cacggtatct gccatcaaca tcgttataaa tattgggatg catcatgcag
     1381 tgatacattg ctgtataaaa aacggttaac ttatttttat cgctgctgct gacttttatt
     1441 ttcgatagtt cttcattcca tttttgtgca gcatttattt ttatgtgttc aaaattccag
     1501 tcgggatttt cgagaatcat atttcgttca gcaccatcat cgtcaacagc agataatgca
     1561 accttagcca tgatactttc tttttcagaa actgaaaaaa cgaacacagc ttttttgttt
     1621 cctaattgtt ttgattgaat aaaaggtttt gaaaaacgaa tgttggcata taggatttgt
     1681 tcctttgccc agccttcgct ttttctgtaa actttaatag tggaatcgta aacaatatta
     1741 atgttacttg ctgttaactt atcacgatga tttaaatcaa gatagatata agctaatccg
     1801 ggtttgttaa atgtataacg atgcattcct gttcttaggg ttgatgttaa ttctacatca
     1861 atactgtcgg caagcaattt tacagaataa taaccggggg tggcattttc gaagttgtgt
     1921 gagaaagttg cagtgggaac actttcatcc aatgcaggct catcgttcat gggtagtaat
     1981 aaaatatcac cataatcact cacaccagta ccactaagat gcgtgtgcga aaatccatat
     2041 aatacagaat cagaataatg ataaccacta catccatccc aacttccatc aatgcgtgtg
     2101 tcaggactca gttgaaccat accgaagggg agtgttgctc ccggaaaagt atgtccatga
     2161 ccacctgttc ctataaatgg atttacatat tgcgtgtaag ttggctgtgc ataaagtatg
     2221 ctgttgcaaa gcaaccagca acttagtaaa gcagttttaa aaaacgacat agaagaagta
     2281 ataatcagat aaaagtataa atagttttga tatagtaaac agaggttatt ttcaggcttt
     2341 tactggaagg aaggtagtgt ataatttatt tcaaattggg ctgttcagcc cattgcaacg
     2401 ggcttgcatt gagtgcacct ttgtaaggtt tttaataaca attgtgaatt acacactatt
     2461 atatttctta gaaaaacagc ataaaccttt tttaatcaca gcttcatctg tattcacgta
     2521 caacaaacca ctgctaactt taagcacttc agaaattttt aacttataat ttttataatt
     2581 aaaataatta aatcaatata aaatgaaaag aacaatacaa tcgctaatct ttattgcagt
     2641 tacattatta accacaaaca tttttgcaca aagttggcag ttagtaggtc cggttggatt
     2701 taccagcttt aatgccaaat atacaaatat tgcattcaat aataatgatg agccttatgt
     2761 ggttttttca gatggttcag ttaatgcaaa agcatcggta atgaaatttg acggcagcaa
     2821 ttgggtgtat gtagggactg ccggattttc tcaaggcatt gcagactata cagatattgc
     2881 ttttgacaat aacggtacac cgtatgtagc atacagtgat catgcaaata atgataatgt
     2941 aacggttatg aaatttgatg gcagcagttg ggtagctgtg ggtaatgttg gattttcaac
     3001 tggtgaacct tattacaatt cattagccat caacagcaat ggagttcctt atgtcgtttt
     3061 ccaagatgca acaagtttat ctaaagcaac agtaatgaag tttgatggca atagttgggt
     3121 tgttgttggt actgccggat tttccagcgt atcggttaaa gagccaacaa ttgcaataga
     3181 taataataac actccttatg tggcctttac acaatggggt ggcttatata atgcatctgt
     3241 tatgaaattt gatggtagta attgggtaaa cgttggtccg gttggtttta caacaaattc
     3301 tgcagactat gcttcattgg catttagcaa tagcggagag ccttatatgg cttttattga
     3361 tggcacgcaa aatagccatg tttctgtaat gaagtttaat ggcagcagtt gggcattggt
     3421 aggaaatgca ggtttttcta caggaacttc acactatcca tctttagcat tcagcaatac
     3481 gggagaaccc tatgtggcat ttaagagtag ctttaacaat tctcaggcta cagtgatgaa
     3541 gtatgatggc agtgcttggg tggctgtggg gtcaacaggc ttttcatcag gatgggcaga
     3601 ttatacatct cttgcattca acagtagcgg tgtgccttat gttgcttttg aggatataaa
     3661 taactcgtat agagcaacag tgatgaaatt tggtaatgga acatcaagca taaacagcat
     3721 ggctgcagat aatagtatag aagtttatcc taatccggct aatgactatc tgaatcttac
     3781 taatcttccg gttggtgcta atatcaaaat caatgatgca ataggcaaga atgtttatga
     3841 tgcttcaatt acaactaagg aaacaagcat caacacctct gcatttacaa atggtat
//
LOCUS       DAOOOI010000149         3882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4157_length_3882_cov_8.255291, whole genome shotgun sequence.
ACCESSION   DAOOOI010000149 DAOOOI010000000
VERSION     DAOOOI010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4157_length_3882_cov_8.255291"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PKX59_10935"
     CDS             complement(<1..260)
                     /locus_tag="PKX59_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91414.1"
                     /translation="MQFRQILIFILCICTIQLQAQTLVNSGFEEDANTSSQKGWITDC
                     KGAGQIKVSNISPFSGKQCLEMAHQNQATCTFSQSIIIENTA"
     gene            453..2534
                     /locus_tag="PKX59_10940"
     CDS             453..2534
                     /locus_tag="PKX59_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOZ91415.1"
                     /translation="MEDAILNAKTNLAPANLSQLGWIKNSISYYYVDNRLADATLFVG
                     DDGIVHDEKIISLSEINDRLKRLKTDTLARFPAVEWKNDKVFGFFIGNKEYEYDKTQY
                     AIRNSRILLLPDSASQKDTDPVTKRIAYTIKNNLFVYDAGKNFTVAEDAYPHIVYGSS
                     VHRNEFGITKGTFWSPNGNKLAFYRMDETMVNDYPIIDWNKKPAEVNMIKYPMAGGTS
                     HQVTVGVFDMANKTVVYLKTGEPKDQYLTNISWSPDEKYVYVAVLNRDQNHMKLNCYS
                     SSTGHFVKTLFEEKDDKYVEPQTPMAFVPGHDDQFIWQSQRDGYNHLYLYNTDGKLIR
                     QLTKGEFIVTEFKGFDKKSERAYYMCTALSPIDRDLYYVTLADGRMKRVSGDSGMHTC
                     YYNPENNEVLDVFSNVNTPRKIKLILPNGSDARTMLTAENPLSDFNLGGMKISTLQSV
                     SGELLYTRTYYPPVMDSTKKYPVIIYVYGGPHLQLITNSWQGGQGDLWFYYLAQEGFI
                     VYTVDSRGSGNRGKQFEQSIFRNCGNAEMEDQLTGLKYLKSLPYVDSTRIGVDGWSYG
                     GFMTISLMTRFPGLFKVAVAGGPVIDWSMYEIMYTERYMDTPATNPEGYEKSNLLNYV
                     KNINGKLMLIHDTNDETVVWQHSLMYLKKAVDLGKQVDYFVYPGHPHNVRGKDRVHLM
                     NKITDYFKQNL"
     gene            2551..>3882
                     /locus_tag="PKX59_10945"
     CDS             2551..>3882
                     /locus_tag="PKX59_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91416.1"
                     /translation="MSKKTIFNLLLCFCVVSIHAQNLKRCPQTTSKEAAKKTEEAEKL
                     LKSVKNYEEGVELCNEAIEADSTFAKPYLLMGDAAYRKKDFKLMAKAYKGLMNVCPDA
                     SEKAIYRLATYYFDTKKYEEAIPVYTTFLELSPSDTNAADDAEVKLFRAKAFLHPVDF
                     KPVLVKGLSTPDPEYLPIISADNELCFFTRRFEYQSKNMLTSTSVEKFMLSHNLNGKW
                     DTGTPMDLPFNKQNTGNEGGPTISLDNLHLFFTVNVKGNFDIYTSDLTPKGWSEPRSI
                     GKANDPKFWDSQPSLSPDNKTLYFASFRDSVLQTSDIYVTKKNNGEWNSANPISGPIN
                     TSRNEKAPFMHPDGKTLYFSSDGHPGMGGYDIYYSKKDANGNWGKPVNLGYPINTDAD
                     EVGFFVSTDGTKGFFASNKFGTGGYDIYSFDMPVEARPEKMVLFKGQLKNDD"
BASE COUNT         1306 a          629 c          770 g         1177 t
ORIGIN      
        1 atcgctgtgt tttcaataat tatactctga ctgaatgtgc atgtagcctg attttgatgt
       61 gccatttcca gacattgttt acctgaaaat ggagaaatgt tactgacttt tatctgacca
      121 gctcctttac aatctgttat ccatcctttc tgagaagaag tattagcatc ttcctcaaag
      181 ccactattta caagtgtctg tgcctgaagt tgtattgtgc aaatgcacaa aataaatatc
      241 agtatttgcc tgaattgcat gattgcaaat aaagaaaatt cagtccaaac attcacaata
      301 tgcagttaat attaatgcat tgtacgaaag aaaagtttat ttataaattt tacatttgct
      361 tgcaagaaga atttattatg ttccgttatt cattagcact ctttttttta atttccacat
      421 caacttcgca ggctcaacac aaaaaattga gcatggaaga tgccatttta aatgcaaaaa
      481 ccaaccttgc tccggcaaac ctttcgcagt taggatggat taaaaattca atttcatact
      541 attatgttga caaccgcttg gctgatgcaa cgctttttgt aggcgatgat ggtatagtac
      601 atgatgagaa aattatttcg ctttcagaaa ttaatgacag actaaaacgt ttaaaaacag
      661 atacacttgc acgttttcct gctgttgaat ggaagaatga taaagtcttt ggattcttta
      721 taggtaataa ggaatatgaa tatgataaaa cacaatatgc cattcgcaat tcaagaatat
      781 tgttgttacc cgacagtgct tctcaaaagg atactgaccc ggtaacaaag cgcatagctt
      841 atacaattaa aaataatctt tttgtgtatg atgcaggaaa aaattttaca gtagcagaag
      901 atgcttaccc acatattgtt tatggcagtt ctgttcatcg caatgaattt ggaataacaa
      961 agggaacatt ttggtcacca aatgggaata aacttgcctt ttacagaatg gatgaaacca
     1021 tggttaatga ttatccgatt atagattgga acaaaaaacc ggcagaagta aatatgataa
     1081 aatacccaat ggcaggtggc acaagtcatc aggtaactgt tggcgtattt gatatggcta
     1141 ataaaacagt tgtttatcta aaaacaggtg agcctaaaga tcaatacctg actaatatat
     1201 catggagtcc tgatgagaag tatgtttatg ttgctgtttt aaatcgcgat cagaaccaca
     1261 tgaaactgaa ttgttattct tcatcaacag gtcattttgt aaaaacactt ttcgaagaaa
     1321 aagatgataa gtatgtagag ccgcaaacac caatggcatt tgtgcccggt catgatgatc
     1381 agttcatctg gcaaagtcag cgtgatggct acaatcattt atatctctat aatactgacg
     1441 ggaaattaat caggcagctt acgaaaggtg agtttatcgt aacggaattt aagggatttg
     1501 ataaaaaatc ggagcgagca tattacatgt gcactgcatt gagtcccatt gacagagatt
     1561 tatattatgt tacacttgca gacggaagaa tgaaaagagt ttcgggcgat agtggaatgc
     1621 atacttgtta ttataatccg gagaataatg aagtgcttga tgtattcagc aatgtcaaca
     1681 caccacgtaa aattaaactt attctgccta atggctctga tgcaagaaca atgctgacgg
     1741 cagaaaatcc gttaagcgat tttaatttag gcggaatgaa aatcagtaca ctgcagagtg
     1801 tttccggtga gttgctttat acacgcacct attatccacc ggtaatggat tcaacaaaaa
     1861 aatatcctgt gattatttat gtttatggtg ggccacattt acaactaatt acaaattcat
     1921 ggcagggtgg acaaggtgac ttgtggttct attatctggc tcaggaggga tttattgttt
     1981 acacggttga tagcagaggt agtggtaaca gaggaaagca gtttgagcaa tccatattcc
     2041 gaaactgtgg aaatgcggag atggaagatc aacttacggg attaaaatat ttaaaatctt
     2101 taccttatgt tgattcaaca cgaataggtg ttgatggatg gagttatggc ggttttatga
     2161 ctatatcact gatgacgagg tttcccggat tgtttaaagt ggctgttgcc ggtggaccag
     2221 taattgactg gagtatgtat gaaattatgt acacagagcg ttatatggat acgcctgcta
     2281 caaaccctga gggctatgaa aaaagtaatt tattgaatta tgtaaaaaat atcaacggta
     2341 agttaatgct cattcacgac acaaatgatg aaacggttgt ttggcaacat agtttgatgt
     2401 atttgaaaaa agctgttgat ctcggaaagc aagttgatta ctttgtttat cccggacatc
     2461 cacataatgt tcgcggtaaa gatcgtgtgc atctaatgaa taaaattaca gattacttta
     2521 aacaaaatct ttgaaaacaa tatttaatta atgagtaaga aaacaatttt taatctttta
     2581 ctatgtttct gtgttgtttc aattcatgca caaaatctaa aacgttgtcc gcagacaaca
     2641 tcaaaagaag cagctaagaa gaccgaagaa gcagagaagc tattaaagtc agttaaaaat
     2701 tatgaggaag gtgttgaatt gtgtaatgag gctattgagg ctgacagcac atttgcaaaa
     2761 ccctatttgc ttatgggtga tgctgcatac aggaaaaaag atttcaagtt aatggcaaag
     2821 gcttataaag gccttatgaa tgtttgtcct gatgcatctg aaaaagcaat ctacagactt
     2881 gcaacatatt attttgatac aaagaagtat gaagaagcta ttcctgttta cactacattc
     2941 cttgaacttt ctccgtcaga taccaacgct gctgacgatg ccgaagtgaa attgttcaga
     3001 gcaaaagcgt ttttacatcc agtagatttt aaacctgtgt tggtaaaagg attaagcaca
     3061 ccagatcctg aatatctgcc aataatctct gctgataatg agttgtgttt ttttacaaga
     3121 agatttgagt atcaaagtaa aaacatgctc acttcgacca gtgtagaaaa gtttatgtta
     3181 agtcataatc taaatggcaa atgggatacc ggtacaccaa tggatttacc ttttaacaaa
     3241 cagaatactg gtaatgaagg tggtccaact atttctctcg acaacctgca cttgtttttt
     3301 actgttaatg taaagggcaa ttttgatatt tatacttctg accttacacc aaaaggatgg
     3361 tctgaaccaa gaagcatagg gaaagcaaat gacccaaagt tttgggattc acaaccatca
     3421 ctttcaccag ataataagac attatatttt gccagtttca gagactctgt tcttcagaca
     3481 agtgatattt atgtgactaa aaaaaataat ggtgaatgga atagtgcaaa tcctatcagt
     3541 ggaccaatca atacttcgcg aaatgaaaaa gcacctttta tgcatcccga tggaaaaaca
     3601 ctttatttca gtagtgatgg tcaccccggc atgggtggat atgatattta ttattccaag
     3661 aaggatgcaa acggtaactg gggaaaacct gttaatctcg gatatccgat aaataccgat
     3721 gcagatgaag ttggattttt tgtaagtact gatggtacta aaggtttttt tgcaagtaat
     3781 aaattcggaa cagggggtta tgatatttac tcattcgaca tgccggtgga agcaaggccc
     3841 gagaagatgg ttttattcaa aggccaactt aaaaatgatg ac
//
LOCUS       DAOOOI010000150         3848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4193_length_3848_cov_21.416030, whole genome shotgun sequence.
ACCESSION   DAOOOI010000150 DAOOOI010000000
VERSION     DAOOOI010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4193_length_3848_cov_21.416030"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PKX59_10950"
     CDS             <1..368
                     /locus_tag="PKX59_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOZ91417.1"
                     /translation="TISKLFEKHFLAIAPPYVKVKVSAHHGGEPVLTPTDSIPFKAAS
                     MAMEKTFGKKPIATRGGGSIPIVALFEKELGLKTVLFGFGLDSDNLHSPNEKYGLFNF
                     YKGIETIPYFFKYYSDLRK"
     gene            486..2540
                     /locus_tag="PKX59_10955"
     CDS             486..2540
                     /locus_tag="PKX59_10955"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HOZ91418.1"
                     /translation="MKSKMILAGCAIALVSLYAFKKKEPGVTGAVKATAATKPIDINN
                     FDMSVKPGDDFYQYVNGNWLKKNPLPASETRWGSFNVLDDITRERVRTILDETAAMKN
                     VVDGSADQKIRDFYLTAMDTIRIEEMRGSPLKPIIEKINKIDSKSQLPTLMAELQRMG
                     FSSFFNFYVGQDDKKSDQMIINLYQGGLGLPDRDYYLKDDKESVEIRQKYLTHVKTMF
                     MLMGIPAGGSSIQNLMTLETELAKISRSRVELRDPEKNYNKMGVQEFAAQMTNFSINE
                     YFKNFGVQEKEVIIGQPEFFKRVNEMIEKTSLDDIKFYLTWDVINSRASTLSSEFVKA
                     DFEFYHGVLSGAKEMRPRWKRVLGTMNGTMGELIGELYVKKYFTPQAKERVNLMVDNL
                     ISAYKERIKSRTWLEDQTKSAALLKLDKVMRKLAYPDRWRDYSKLSVGKDSYCDNVNR
                     ASEFWFQYNLSKLGKPVDKSEWAMSPQTINAYYNPGFNEIVFPAAIMQPPFFDPMADD
                     AVNYGAMGAVIGHELTHGFDDQGCQYDAEGNLKNWWTEKDKSLFDQRAQKLVDQFNHY
                     MPIDSLTINGKLTLGENIADLGGLTIAYAALQKTLALKPKVKIDGFTPEQRFFINFAQ
                     VWRNNIRPEELKKRLKTDPHSPGKFRANGTVRNMPEFYEAFGITKENKMYLPPDQRVE
                     IW"
     gene            complement(2739..3281)
                     /locus_tag="PKX59_10960"
     CDS             complement(2739..3281)
                     /locus_tag="PKX59_10960"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HOZ91419.1"
                     /translation="MTTFISILRGINVSGHRLIKMDALKELCASLKMQQVKTYIQSGN
                     IVFQSVQGDCDAISKKLEHAILKKFGFAVPVITLSQYEFKSCIEANPFLKDKKLDAAF
                     FHVTFLSEQPSIHDSIETKAVNLKNDLFVFGERCIYLYCPDGYGNTKLTNSFFESKLK
                     VTATTRNWKTVNELLRLAEM"
     gene            complement(3290..>3848)
                     /locus_tag="PKX59_10965"
     CDS             complement(3290..>3848)
                     /locus_tag="PKX59_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOZ91420.1"
                     /translation="QLDKNDIEAIIAFIRTLKPIENKTEISSSDFPMNFIINTIPKKP
                     SFTTIPPQSDKLNYGKYIATASGCMECHTKQVKGKIVGQPYAGGFEFMLPGNTVVRSA
                     NITPDKETGIGTWTNQQFVDRFKMYSDSVYTNLKVAPGTLQTTMPWTMYAGMKQEDLS
                     ALFAYLQSLPPVKNTVEKFTVTSGK"
BASE COUNT         1261 a          651 c          738 g         1198 t
ORIGIN      
        1 aaacaatttc taaactattc gaaaaacatt ttcttgctat tgcaccacct tatgttaaag
       61 taaaagtgtc tgcacatcat ggaggcgagc ctgttctgac accaacagac agcattcctt
      121 ttaaagcagc aagcatggca atggaaaaaa cttttggtaa aaaaccaatt gccactagag
      181 gtggtggaag tattcctatc gttgcattgt ttgaaaagga attgggttta aaaacagtgt
      241 tgtttggttt tggattagat agcgataacc tgcattctcc aaacgaaaaa tatgggctgt
      301 ttaacttcta taaaggtata gaaacaattc catatttctt caaatattac agcgatttaa
      361 gaaagtaaca atttcatctc tgtaatcttt atttctaaaa cctttgaaaa ggtttttcaa
      421 taatgaagta agtgacattt attatttaat aaaaatatag ttttaaaatc agtaattcaa
      481 taaaaatgaa aagtaaaatg attcttgcag gatgtgccat tgctttggtg tcgctctatg
      541 catttaaaaa gaaagagccc ggagtcacgg gtgctgtaaa ggcaacagca gcaacaaaac
      601 caattgatat aaacaatttt gatatgagcg taaaaccggg agacgacttt taccagtatg
      661 ttaatggtaa ctggctgaaa aaaaatccac ttcctgcctc tgaaacacga tggggaagtt
      721 ttaatgtgct tgatgatatt acacgcgaac gagtgcgcac catcctcgat gaaactgcag
      781 ccatgaaaaa tgttgttgat ggtagtgccg atcagaaaat acgtgatttt tatcttacag
      841 caatggacac cataaggatt gaagaaatgc gcggatctcc attaaaacct atcattgaaa
      901 agataaataa aatagattca aaatcgcaac tcccgacatt aatggcagaa ctacagcgta
      961 tgggctttag tagttttttt aatttttatg ttggtcagga tgataaaaaa agcgatcaga
     1021 tgattattaa tctttatcag ggtggattag gattgcccga tagagattac tatctcaaag
     1081 acgataaaga atctgtagaa atcagacaga aatatcttac acatgttaaa accatgttta
     1141 tgctcatggg tattcctgca ggaggcagtt ctattcaaaa cctgatgaca cttgaaacag
     1201 aacttgcaaa gatttctcgt tcaagagtgg aactacgcga cccggaaaag aattataata
     1261 agatgggtgt tcaggagttt gctgctcaga tgacaaattt ttcaattaat gaatatttca
     1321 aaaattttgg agtgcaagag aaagaagtta tcattggtca gcccgaattt tttaaacgtg
     1381 tcaatgagat gattgagaaa acatcgctcg atgacattaa attttatctt acatgggatg
     1441 tcattaacag tcgtgcaagt acattaagtt ctgaatttgt aaaagccgat ttcgagttct
     1501 atcacggtgt tttaagtggt gcaaaagaaa tgcgtccacg ctggaaacgt gttcttggaa
     1561 ctatgaatgg cacaatgggt gagttaatag gggagttgta tgtaaaaaag tattttacac
     1621 cccaagcaaa agagcgtgta aacctgatgg tggataattt aattagtgct tataaagaaa
     1681 gaatcaaatc gcgcacctgg ctcgaagatc aaaccaagtc ggcagcttta ttaaaacttg
     1741 ataaagtaat gcgcaaattg gcttaccctg atagatggcg cgactacagt aaattatcag
     1801 taggcaaaga ttcatactgc gacaatgtta accgcgcttc tgaattttgg tttcaatata
     1861 acttatccaa attaggaaag cctgttgaca agtcagagtg ggcaatgtcg ccacaaacca
     1921 tcaatgcata ttacaatccc ggatttaatg aaattgtttt tcctgctgca ataatgcaac
     1981 caccattctt tgatcctatg gctgacgatg ccgtaaacta tggtgctatg ggtgccgtta
     2041 tcggacatga gttaactcat ggttttgatg atcagggttg tcagtatgat gctgaaggta
     2101 acttaaaaaa ctggtggaca gaaaaagaca aatctttatt cgatcagaga gcacaaaagt
     2161 tagtggatca gttcaaccat tatatgccta ttgactcact caccattaat ggcaaactta
     2221 ctcttggtga aaacattgcc gatttaggcg gactaactat tgcctatgct gcattacaga
     2281 aaacgcttgc cttaaagcct aaagtaaaaa ttgacggctt tactccggaa caacgtttct
     2341 ttatcaactt tgctcaggta tggcgcaata acattcgccc cgaagaatta aaaaagagat
     2401 taaaaacaga tccgcacagc ccgggaaaat tccgtgctaa tggcactgta cgtaatatgc
     2461 ctgagtttta tgaagcattt ggtattacaa aagagaataa aatgtattta cctcccgacc
     2521 aaagagtaga aatctggtaa tatttattac agaaatttca gaaggctgca ttcaacaagt
     2581 gcagcctttt ttgtttaaat attttatatg tgttcaaaca ttgtggtcgg caataaaatt
     2641 ttacgacaaa tttattgatg tttattgctc caataggctt gtggtatatt caatcaagag
     2701 tgaaaagaat tgatgaagct tctaaaaata aatctgattt acatctcagc caaccgcaac
     2761 agctcattta cagttttcca gtttctggtt gtagctgtaa ctttaagttt gctttcaaaa
     2821 aaactatttg tcagcttagt gtttccatat ccatcggggc agtagaggta aatgcacctt
     2881 tcaccaaaaa cgaaaaggtc gttttttaga ttgacagctt ttgtttctat cgaatcatga
     2941 atgcttggtt gttctgaaag aaaggtgaca tgaaaaaatg ccgcatccaa ttttttatct
     3001 tttaaaaaag gatttgcctc tatgcaactt ttaaattcat actgcgacaa tgtgatgaca
     3061 ggtactgcaa atccaaattt ttttaaaata gcgtgttcta actttttgct tatagcgtca
     3121 cagtcacctt gtacagattg aaaaacaatg ttgccgcttt gaatgtaggt tttcacttgt
     3181 tgcattttta aagaagcaca aagttctttt aatgcatcca tttttatcaa ccgatgtcca
     3241 ctgacattta tgcctcttag aatagaaata aaagtggtca ttaaaattgt tacttaccgc
     3301 ttgtaactgt aaatttttca acagtatttt ttaccggtgg tagactttgc aaataagcaa
     3361 acaaagctga aaggtcttct tgcttcatgc ctgcatacat tgtccatggc attgttgttt
     3421 gcaatgtgcc gggtgctact ttcaaattag tgtaaacgct gtcagaatac attttaaatc
     3481 ggtcaacaaa ctgctgattt gtccaagttc ctatacctgt ttctttatcg ggagtaatat
     3541 tggcagaacg aacaacagta ttgcccggta gcataaattc aaatccaccg gcatagggct
     3601 gaccaacaat ttttcctttt acttgtttgg tatgacattc catgcatcct gatgctgtgg
     3661 caatatattt tccgtaattc aatttatcac tctgtggagg aatagttgta aatgatggtt
     3721 tctttggaat agtgttgata ataaaattca ttggaaaatc ggaagatgat atttctgttt
     3781 tgttttcaat tggttttaat gtgcggataa aagcaatgat agcttcaata tcatttttgt
     3841 ctaactga
//
LOCUS       DAOOOI010000151         3845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4199_length_3845_cov_17.376253, whole genome shotgun sequence.
ACCESSION   DAOOOI010000151 DAOOOI010000000
VERSION     DAOOOI010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4199_length_3845_cov_17.376253"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1889)
                     /locus_tag="PKX59_10970"
     CDS             complement(<1..1889)
                     /locus_tag="PKX59_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91421.1"
                     /translation="MKRVANLFISLAFLLYVKAASAQLTLAEWNFPNNPDNATVDISI
                     PANSAKTIYTVGGTAAVVYTTAGATTQSAWTTGWDNGSGTKYWETEINTTGYFNLELS
                     SKQRSSPTGPKNFAIEYKIGIAGTWTPIPGAGAIVVADNFTSGVVTDIPLPVACENQA
                     SVFLRWIMTTNTSVNNSTVASGGSSRIDDVIIKANDAQNHYRSITSGAWNSLSTWQQS
                     PDLVTWTAAVFLPNYYAKTITIRSGHTVTISANVRIDETTINNGGTVNYNSKLLTINN
                     GTGVDLQVNGTFTDGSSTSVAWLAGATWALGASGTYVKTENTNATNWQNNYQGGISTI
                     PATASWVLNKTPSSVTAPSLTTLNMFYPNLTIMNTSGGVWTTAVSSTFTGSTATATIK
                     GNFDVGGVLAPLLGTVDFLNDNNFATPVQVQGNILIRGGCNLRNFGTGFEIFGNLTVN
                     GSTSYGAANARKYRFSGGNAQSISGSAALTSLQIYQLEMNKSANSLTLLKSVKVDNNL
                     NLITGIINSSTPNILIVENGATATGASNVSFVNGPVQKNGSQAFTFPVGKNTSYRSIA
                     IGADASSSSFWTENFNTGAGWSLVNVMGPEGADPNPFVISDAEGGVAAGGCGVANNGN
                     NTMHV"
     gene            complement(2152..2460)
                     /locus_tag="PKX59_10975"
     CDS             complement(2152..2460)
                     /locus_tag="PKX59_10975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91422.1"
                     /translation="MKLKKGKRKFAFDFVHPAFKIESNSTLLNIFPNPSSGVFNVQSD
                     NQLENVQITVTDLQGRTLINTLFSGNETTVDLSNYTQGVYLLQLHSSNVVQTYKLIKD
                     "
     gene            complement(2917..>3845)
                     /locus_tag="PKX59_10980"
     CDS             complement(2917..>3845)
                     /locus_tag="PKX59_10980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91423.1"
                     /translation="SNQFVASDPDIFILKLDSTGNFIWAQNFGATSEDVGQAISVDAN
                     GNVFTTGYFEDAVDFEWGNGTFNLTTSIFEDVFVLKSDAAGNFLWAKQIVSSSFYDAG
                     NGIATDVAGNVYITGSFAGTADLDPGIDSAFVTAASSSPDIYILKLDAGGNFLWGGRA
                     GDIGNDAGEEIVTDASGNIFTAGSFSRTADFDFNTGVNNLTTVNTLSNVYVLKMNQNN
                     VGIYENMIQNKFTVYPNPATNILKINNLKFEIGSAIKIFDVMGKEVLHTVVGGAETAI
                     NVSKLESGIYFVHLFSVSAPEENILTEKIIIH"
BASE COUNT         1197 a          716 c          715 g         1217 t
ORIGIN      
        1 ctaacatgca tggtattgtt tccattattt gcaactccac aaccacctgc agcaacacca
       61 ccttctgcat cacttattac aaaaggattt ggatcagcac cttcgggccc cataacgtta
      121 acaagagacc agcctgctcc ggtattaaaa ttttctgtcc agaaagatga cgatgatgca
      181 tcagcgccaa tagcaattga ccgatatgaa gtattttttc ctacaggaaa agtgaatgcc
      241 tgtgagccgt ttttttgcac cgggccattt acaaaactaa catttgatgc tcctgttgca
      301 gttgcaccat tttcaactat taaaatattg ggagttgaag aatttataat acctgttatt
      361 aagttcagat tgttatcaac ctttactgat tttaaaaggg taaggctgtt ggcagactta
      421 ttcatttcaa gttgataaat ctgtagcgat gtaagtgctg cagagcctga tatagattgt
      481 gcattaccac cactgaatct atacttacgt gcattagctg caccataact tgtggagcca
      541 tttacagtta ggtttccgaa aatttcaaac cctgttccga aatttctgag attgcatcca
      601 ccacgtatta gtatattgcc ctgaacttgt accggtgtag caaaattgtt atcatttaaa
      661 aaatcaacag tgcctaatag tggagctaac acaccaccaa catcaaaatt acctttgata
      721 gttgcagtag ctgtgctgcc tgtgaatgtt gatgaaacag cagttgtcca aacacctccc
      781 gaagtattca tgatagtgag gtttggataa aacatattta aagttgttaa tgaaggtgca
      841 gtaactgaac tgggtgtttt atttaaaacc caacttgcag ttgcagggat agtgcttatg
      901 ccaccctgat aattgttttg ccagtttgtt gcatttgtat tctctgtttt aacataagtg
      961 cctgatgcac ctaatgccca tgttgctcca gctagccagg caacgctggt tgaacttcca
     1021 tctgtaaatg taccgttaac ctgtaagtca acacctgtac cattattgat tgtaagcagt
     1081 ttggaattgt aatttacagt tccaccattg ttaatagttg tttcatcaat gcgtacattg
     1141 gccgaaatgg ttactgtatg tccactgcga atggtaatag ttttagcata gtagtttggt
     1201 aaaaaaacag ctgctgtcca cgtaactaaa tccggtgatt gttgccatgt tgataatgaa
     1261 ttccatgcac cggaagtgat actgcgataa tgattttgtg catcgttggc tttaataatt
     1321 acatcatcaa ttcttgatga accaccggaa gccacagtgc tgttgttaac acttgtattc
     1381 gttgtcatta tccaacgtaa aaaaactgaa gcttgatttt cacatgcaac aggtaagggg
     1441 atatcggtga caacacctga agtaaaattg tctgccacaa caattgcacc ggcaccggga
     1501 attggtgtcc atgtgccggc aataccaatc ttatattcta tagcaaagtt cttgggccct
     1561 gtgggcgaac tgcgttgttt agaggataat tcaagattaa aataacctgt tgtattaatt
     1621 tctgtctccc aatattttgt gccggagcca ttatcccatc ctgttgtcca agcactttga
     1681 gttgtggcac cggcagttgt ataaacaact gcggcagtac ctcctacggt gtaaatggtt
     1741 tttgcactat tagcaggaat agaaatatct acagtagcat tatcaggatt gtttggaaaa
     1801 ttccattcag ccaatgttaa ttgagcagaa gcggctttga cataaagtag aaatgccaat
     1861 gaaataaata aattggctac tcttttcatg ttgtttttta ttgtaaaatc aaagatatga
     1921 gaaattatta ctaatccaac atttaataga taaaacttat gaaagtaaaa aactgataat
     1981 aggatgaagg cataaggggg tgaatgttgc agaaaaggat atccttaaaa accggagatt
     2041 ttaaaggaga ttatgcaatg agtatatagg aatggtgcct tcaagaaagg cttacaaaaa
     2101 aacaaggctg cccaatcggg aagccttgtt gtaagagaat tatggggtta attaatcctt
     2161 tatcagttta taagtctgaa ccacattgct tgaatgtaat tgcaacagat aaactccttg
     2221 agtgtagtta gataaatcaa cagtagtttc attgcctgaa aacaaagtat taatcaatgt
     2281 acgaccttgt aagtctgtta ctgttatctg aacattttca agttgattgt cggattgtac
     2341 attaaatact cctgaagaag gatttgggaa gatgttgagt aatgtgctgt tgctttcaat
     2401 tttaaaagct ggatgaacaa aatcaaatgc aaattttctt ttgccttttt tcaatttcaa
     2461 cttcaaaccc gaattttgat ggcaatcctg tttttaaaat ctcattggag ggttgttaag
     2521 gattaactaa ctaaataaac agtattgtta ttgtcaatat gtaacgagtt tacagagaag
     2581 caagtgttta ttgcccagac aaaatttcct gctgcatcaa atttgattaa gaaaattgca
     2641 ttgacgttgt taccgcctgg tgctgtcaga tttaaaacag ctgggcccgg attaaagtca
     2701 acagttcaga aaagaactgg ttacataaag gtcaccgtta aagggatctt ttgtaattga
     2761 ttttcctttt tcattagagg ccactcccat agtctttacc catgataaag atggcatttg
     2821 tgaatttgat attgtaaatg atacaacaaa caaacatgcc attaatcagg atactgattt
     2881 gttgtaaaat ttattcatga cttttcagtt tatattttag tgaatgataa ttttttctgt
     2941 taagatattt tcctccggag cactaacaga aaataaatga acgaaataaa ttccactttc
     3001 taatttgctt acattaattg ctgtttctgc acctccaaca actgtatgta aaacttcttt
     3061 tcccatcaca tcaaaaattt ttattgcact gcctatctca aactttaaat tgtttatctt
     3121 taaaatgttt gttgcagggt tggggtaaac ggtaaacttg ttttggatca tgttttcata
     3181 tatgcctaca ttattttgat tcatcttaag cacataaaca ttggaaaggg tattgacagt
     3241 agtaagatta ttaacaccgg tattaaaatc aaaatcggca gtgcggctaa aactgccagc
     3301 ggtaaaaatg ttgccggatg catcggtaac aatttcttct ccggcatcgt tacctatgtc
     3361 accggcacgg cctccccata aaaaattgcc gcctgcatct aattttaaaa tataaatatc
     3421 ggggctgctg gaggcagcag ttacaaaagc agaatcaata ccggggtcca aatcagcagt
     3481 gcctgcaaac gaacctgtta tatatacatt gccggcaaca tctgttgcaa tgccattgcc
     3541 tgcatcataa aacgaactac ttactatttg ttttgcccat aagaaattac cggcagcatc
     3601 tgattttaaa acaaaaacat cttcaaaaat actggtggta agattaaaag tgccattgcc
     3661 ccattcaaaa tctactgcgt cttcaaaata acctgttgta aatacattgc cattggcatc
     3721 aacacttatt gcctggccca catcttcgga tgttgctcca aaattttgcg cccaaataaa
     3781 attgcctgtt gaatctaact ttaaaataaa aatatcgggg tcgcttgcca caaactgatt
     3841 gctgc
//
LOCUS       DAOOOI010000152         3766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4300_length_3766_cov_5.831312, whole genome shotgun sequence.
ACCESSION   DAOOOI010000152 DAOOOI010000000
VERSION     DAOOOI010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4300_length_3766_cov_5.831312"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PKX59_10985"
     CDS             complement(<1..195)
                     /locus_tag="PKX59_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91424.1"
                     /translation="MSGQLALTWQHQWKDKRFKTAFVFIPKSISESKYWLQEGSYQLG
                     GAILQSYQRSEQLSYKLGLYY"
     gene            171..1133
                     /locus_tag="PKX59_10990"
     CDS             171..1133
                     /locus_tag="PKX59_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOZ91425.1"
                     /translation="MLVQADHSYKVLIPVAGKPIVQHLMEDIISVCPEKVDEIAFVVG
                     RFGSQVEKTLTDIAASLGAKGTIHYQDEPLGTAHAILCAQSALKGKVVVAFADTLFKS
                     NAKIDSAHDGTIWVKQIEDPRQFGVVKVNAENIITDFVEKPVQFVSDLAIIGIYYFKD
                     GENLKKELQYLIDNNIKDKGEFQLTNAMENMKNKGLKLVPGKVEEWLDCGNKDATAYT
                     NGRMLDIKYPENYISPSAKITNSVINKPCFIADNAEIIGSVIGPGVSVGAKTKIENSV
                     ISQCIIQTNSNINDAVISNSMIGNYCVYKGKADDLSMGDYSTQA"
     gene            1144..>2221
                     /locus_tag="PKX59_10995"
     CDS             1144..>2221
                     /locus_tag="PKX59_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ91426.1"
                     /translation="MRSFLEVRKVLVLLLMIGVFATSCKTSKQTTSAGKETNLSEESK
                     TKLMYFFVNANKEKILGNEGRAAELFAECLKIDPRNDASLYEMAQLYANRKKYAEALN
                     FAKSASSINPKNEWYKQLLAEIYQRTNQWNEAALIYQNLTAEFPQRIDFYYQWAYALV
                     NASKLNDALKAFDKIESIVGPDKEITLRKEQIYLKQNKVDKAAEVLENYISSNPESMD
                     MYSLLYELYSANNMPDKALSVIDRMKAVNPNEPRIYLNLADYYRNKGDKEKSFENLQK
                     AFTNRDLDTDIKVKIISSYIPLLQRDSSMMLQCMELCKTLAETHPDESRAQAIYGDLL
                     TMNKDYSQAVSFYRKALDLDKDNLN"
     assembly_gap    2222..2259
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2260..>2483
                     /locus_tag="PKX59_11000"
     CDS             <2260..>2483
                     /locus_tag="PKX59_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91427.1"
                     /translation="DTASAEAIGLFPNESSLYLLNGIAKSQRNLPADAINILNQGVKL
                     VVDNNQQLIQFYSNLGDCYNKTKEYSASDS"
     assembly_gap    2484..2494
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2495..2866
                     /locus_tag="PKX59_11005"
     CDS             <2495..2866
                     /locus_tag="PKX59_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91428.1"
                     /translation="IDPNEPFVLNNYAYYLSVRKEKLEQAEKMSKHSNELQPNQSSFL
                     DTYGWILYQEGKYDEAKKWIEKAMENGGENSGTILEHYGDILFKLNQSDKAFEYWKKA
                     KQSGDASGLLDQKIRDKKLYE"
     gene            2859..3695
                     /locus_tag="PKX59_11010"
     CDS             2859..3695
                     /locus_tag="PKX59_11010"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOZ91429.1"
                     /translation="MSKFLQIILCVLIPMLVFNACKVTKKSEKPIVIKPITKLEEQGA
                     ETLLKNIIDSAFFGRWISGKATVNTNIDGDKNNFNVSLRICRDSAIWISVSPLLGIEA
                     VRILLTPDTVRFLDRINKEYKVSGYKEINDLLYAGNLDFDIIQGILTGSLFVYKRNKF
                     HSVYLEDQYYILSTLSKRKLKRSLEEKDPSKPVIQDFYVDGSNYRIMKLAIEDNRIQK
                     SLETKYSGYSYTSAGYFPHHSVTNIKAEKNILVDLEYTKVTVEDTLEFPFSIPKSFKK
                     AR"
BASE COUNT         1294 a          607 c          736 g         1080 t
ORIGIN      
        1 gtaatataat ccgagtttgt aacttagttg ctctgagcgc tgatagcttt gtaaaattgc
       61 tcccccaagc tgatagctgc cctcttgcag ccaatattta ctttcggaaa tagatttcgg
      121 aataaacaca aaggcagttt taaaacgttt gtctttccac tgatgttgcc atgttagtgc
      181 aagctgacca ctcatataaa gttttgattc ctgttgcagg aaaacctata gttcagcatc
      241 ttatggaaga tattatttct gtttgccccg aaaaggttga cgaaattgct tttgtagtag
      301 gtcgttttgg tagtcaggta gaaaaaacgc tgacagatat agcggcatct ttaggtgcta
      361 aaggaacaat acattatcag gacgaacctc tgggaactgc acatgcaata ctttgtgcac
      421 agtctgcgct taaaggaaaa gtagttgttg catttgctga cacacttttt aaaagtaatg
      481 ctaaaataga ttctgcgcat gatggaacca tttgggtaaa acagatagaa gacccacgtc
      541 agtttggagt agttaaagta aatgcagaaa atattataac agactttgtt gagaaaccgg
      601 tacagtttgt ttctgatctg gcaattatcg ggatttacta ttttaaagat ggcgaaaacc
      661 tgaagaaaga acttcagtat ttgatagata ataatattaa agacaaagga gagtttcagt
      721 taaccaatgc catggagaac atgaaaaaca aaggactgaa attggttccc ggtaaagtag
      781 aggagtggct cgactgtgga aataaagatg ctactgcata taccaatggt agaatgttag
      841 acattaaata tcctgaaaat tatatttcgc catcagcaaa aatcacaaat agtgtaatca
      901 acaaaccatg ttttattgca gacaatgcag aaattatcgg aagtgtgatt ggccccggag
      961 tgagtgttgg tgcaaaaaca aaaattgaaa acagtgtaat ttcacaatgt attattcaaa
     1021 ctaacagcaa catcaacgat gctgttatca gcaattcaat gataggaaac tattgtgttt
     1081 ataaaggtaa ggccgatgat ttgagtatgg gtgattattc aacacaggca taataaaaat
     1141 aagatgagga gttttttaga agttcgtaaa gttttggttt tgctcttaat gattggcgtg
     1201 tttgccacat catgtaagac aagtaaacaa acaacttctg caggcaagga aaccaatctc
     1261 tccgaagaga gtaaaacaaa actcatgtat ttctttgtga atgccaacaa agaaaaaata
     1321 ttgggtaatg aaggtcgtgc agctgaactt tttgcagagt gtttaaagat tgatccgaga
     1381 aatgatgcct cactttatga aatggcgcag ctatatgcca atcgtaaaaa atatgctgaa
     1441 gcattaaact ttgcaaaaag cgcatcaagc attaatccaa agaatgaatg gtataagcaa
     1501 ctgctggcag aaatttatca aagaacaaat cagtggaacg aagcggcatt aatttatcag
     1561 aatcttaccg ctgagtttcc acaacgaatt gacttttatt atcagtgggc ttatgcattg
     1621 gtgaatgcat ccaaactgaa tgatgccttg aaagcttttg ataaaatcga atctattgtt
     1681 ggccccgata aagaaatcac cttacgtaaa gagcaaattt atctgaagca aaacaaggtt
     1741 gacaaagccg ctgaagtact tgaaaattat atcagcagca atccggagag tatggatatg
     1801 tactctctgc tttatgagct ttattcagca aacaacatgc ccgacaaagc attgtctgtt
     1861 atagatagaa tgaaggctgt taatccgaat gaaccacgca tttatctcaa tctggcagat
     1921 tactatcgca acaaaggtga taaagaaaaa tcattcgaga atttacagaa agcttttacc
     1981 aatcgcgact tagatacaga cattaaagtt aaaataattt cttcttacat tccattgttg
     2041 caacgcgatt catcaatgat gttgcagtgt atggagttgt gtaaaacact tgcagaaacg
     2101 catcctgatg aatcacgcgc acaggcaatt tatggtgatt tgttaactat gaataaagat
     2161 tatagccagg ctgtttcatt ctacagaaaa gcacttgatt tggataaaga taatctgaat
     2221 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gacacggcaa gtgccgaagc
     2281 aatagggttg tttccaaatg aatcttcact ttatctgtta aacggtattg caaaaagtca
     2341 gcgcaattta cctgctgatg caattaatat tttaaatcaa ggtgttaagt tggtggttga
     2401 caacaatcag caattgatac aattttattc taatctcggt gattgttata ataagactaa
     2461 agagtattca gcctctgaca gttnnnnnnn nnnnattgat cccaatgagc cttttgtgct
     2521 caataattac gcctactatc tatcggttag aaaagaaaaa ttagagcagg cagagaagat
     2581 gtctaagcat agtaatgaac ttcaacccaa tcagtcatca tttttagata cctacggatg
     2641 gatattgtat caggaaggaa agtatgatga agcaaaaaaa tggattgaaa aagctatgga
     2701 aaacggaggt gaaaacagcg gtactattct ggaacattac ggtgacattt tatttaagct
     2761 gaatcaatcc gataaagcat ttgaatactg gaaaaaggca aagcaatcgg gagatgcatc
     2821 aggattatta gaccagaaaa tcagggataa aaaactgtat gagtaaattt ctacagataa
     2881 ttttatgtgt gttgatacca atgctggtat ttaatgcctg taaggttacc aagaaatcag
     2941 agaagccaat tgttattaaa cccataacaa aacttgagga gcaaggagct gaaactttat
     3001 tgaaaaatat tattgacagt gctttttttg gaagatggat tagcggtaag gcaactgtaa
     3061 atacaaacat tgatggcgat aaaaataact ttaatgtttc gctgcgtata tgccgcgaca
     3121 gtgctatctg gatttctgtt tcgccactgc tcggcattga agccgttcgc atattgctga
     3181 cacctgatac agttcgtttt ctcgacagaa tcaataaaga gtataaagtt tccggctaca
     3241 aagaaattaa cgatttgctt tatgcaggca acctcgactt tgatattatc cagggtattc
     3301 tgacaggaag tctttttgtt tataaaagaa ataaatttca ctctgtttat cttgaagatc
     3361 agtattacat tctcagtaca ctcagcaagc gtaaattaaa acgatcgctt gaagagaaag
     3421 acccgagtaa acctgtcatt caggattttt atgttgatgg aagcaactat agaattatga
     3481 agttagccat tgaagacaat cggatacaaa aatcgcttga gacaaaatat tccggttatt
     3541 catatacctc tgccggatat tttcctcatc attctgtaac caatattaaa gcagaaaaaa
     3601 atattcttgt tgacttggaa tatactaaag taactgttga agacacattg gaatttccat
     3661 tcagcatacc taaaagcttt aagaaagcgc gctgattgtc gtgcataaat gtgtcaataa
     3721 ttaatttgca tcttaaagac taaagggaac actaaaccta attttg
//
LOCUS       DAOOOI010000153         3758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4309_length_3758_cov_8.442344, whole genome shotgun sequence.
ACCESSION   DAOOOI010000153 DAOOOI010000000
VERSION     DAOOOI010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4309_length_3758_cov_8.442344"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..719
                     /locus_tag="PKX59_11015"
                     /pseudo
     CDS             1..719
                     /locus_tag="PKX59_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631362.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
     assembly_gap    720..733
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <734..1130
                     /locus_tag="PKX59_11020"
     CDS             <734..1130
                     /locus_tag="PKX59_11020"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788591.1"
                     /note="Acs; catalyzes the conversion of acetate and CoA to
                     acetyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HOZ91430.1"
                     /translation="VDDVLNVSGHRIGTAEVENAINMHQGVVESAVVGFPHDIKGQGI
                     YAFVIFNGMHVDEEHTRQDVIQTVSRIIGAIAKPDKIQFVSGLPKTRSGKIMRRILRK
                     IAEGELNNLGDTTTLLDPGVVEEIKSGRL"
     gene            1233..1490
                     /locus_tag="PKX59_11025"
     CDS             1233..1490
                     /locus_tag="PKX59_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91431.1"
                     /translation="MNRIVVSSIISIVIIGLMAVGYYYFSAYREKFSATEDALPSDAA
                     IVIRGSMGDLYASLEKVGLWNERDSLTVMYRFKKRDAHFAE"
     gene            1471..>2919
                     /locus_tag="PKX59_11030"
     CDS             1471..>2919
                     /locus_tag="PKX59_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91432.1"
                     /translation="MHTLLSNTTSMFDVADLLESPQTTIAVLATGARNAELLYLVPSR
                     KELNIDALQKALTGQLSENKSRTFAGVSIYELQFSNFSSSFTFAFANGIFIGSFNAGL
                     VEDAIRQQATGKPFGGNVSAQKLYKEIAVSSGTILGLNYPALKKLFSVAISSDNVIEL
                     NNLEKWTEWSFHNLTFDGNSINANGSVLVSDSTQQIADFGNVRSSTDLLWSVLPDDVS
                     ALQIFSFKDALIWSDKNQRRNADNEVIINYIKQVSVTETKLRVEIRKDFSTIVGEIGA
                     IAINGNPTSLLENNSVAILQLKDQKKSLTQLLALSKKLSKNKDVIAVEKFRNHEIRFL
                     NCDGLLPAMFGNAFNVIRKNYFTTHNGFVIFANKPSQLRGFIDDVEDNEMLNKNEVYQ
                     QNKSVLSESLSYALFLRPTNLNSYFQSVANSNTLKALAASDLMYKFSSFYYYIQPGIS
                     GKVDCNFRIVIQQQKQQKLSEPKWSFFTDSAIH"
     assembly_gap    2920..2939
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2940..>3758
                     /locus_tag="PKX59_11035"
     CDS             <2940..>3758
                     /locus_tag="PKX59_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91433.1"
                     /translation="PYIFTSDNGISILFQDSTKSLICLDGSGNVSWKTTISGFINGEI
                     SQTINDQSGMIQFICNTNDSMYMFNQNGVMPAHYPFKLPVHSRYGIVASPNTNVLFVA
                     GENNLMYAFDPGSRPSQNWNTVQFEGNLFNASIDALGRFVYIMDGDKLRLYSTDGKLI
                     LSKPSTEFRVNSMQWVNDSVPSLFMLNNDGKIVVWNIDGTYKIASSDSTFDFAETCGS
                     GTARIWYTIKGRELNILDYTFKAVENVDLSKVEFNNMQLFNTGNEILCILKGSE"
BASE COUNT         1207 a          572 c          761 g         1184 t
ORIGIN      
        1 gcctaaagga gtagtacata cagtagccgg atatatggtg tggacaaact acacttttgt
       61 caatgtgttt caatacaaac ccggacaaat ttatttctgt actgctgaca taggttggat
      121 tacaggacat agttatctgc tctatggccc tttaagtgca ggagcaacaa ccatgatgtt
      181 tgaaggaatc ccgacatttc ctgatgcagg tcgtttttgg gatattacgc agaaacacaa
      241 agtagatatt ttatatacag cacctactgc tattagaagc ttaatggctt atggaacttc
      301 ttatctcgaa gggaaagatt taagttcact taaagtgctt ggtactgttg gtgagccaat
      361 caatgaagag gcttggtatt ggtactatga gaatgtaggc aaaagtaaat gtccaattgt
      421 tgatacctgg tggcaaactg aaaccggtgg cataatgatt tcgaatcttg caggcataac
      481 gcctcacaaa cctacttatg ccacattgcc gatgcccggt gtgcttccat gtcttgttga
      541 tgaaaccggc aatgaactta ttgataatgg tgtcagcggt aatttatgta ttaagcaggc
      601 atggcctgga atgattcgta ccacttatgg tgatcatgaa cgttgccgtc agacttattt
      661 ttcgacttat aaaaacatgt actttacagg tgatggctgt ctgcgcgata aagatggtan
      721 nnnnnnnnnn nnntgttgat gatgtgctta atgtcagtgg tcaccgtata ggcactgccg
      781 aagtagagaa tgcaattaac atgcatcagg gagttgttga aagtgcagta gtaggttttc
      841 cacatgatat aaaagggcag ggtatttatg cctttgttat tttcaacgga atgcatgttg
      901 atgaggaaca cacaaggcaa gatgtaattc agacagtgtc aagaattatt ggtgccattg
      961 ctaagcctga taaaattcag tttgtttcag gacttcctaa aacacgcagc ggaaaaatta
     1021 tgcgaagaat attacgcaaa attgctgaag gagaattaaa taatcttggc gatactacaa
     1081 cacttctcga tccgggtgtt gttgaagaaa ttaaatccgg aagattgtaa acagtgcttg
     1141 ttgtcaatta acattatctt gtaaataaca ttaaacacta ctaatgtttc tccgagtagt
     1201 gaagctatag ttttgactca caagttttat ttgtgaacag gattgtagtt tcttccataa
     1261 tttcaattgt catcataggc ctgatggctg taggctatta ttatttttct gcctataggg
     1321 aaaaattttc agctacagaa gatgcattgc ctagtgatgc tgccatagta atcagaggta
     1381 gtatgggcga tttgtatgct tctttagaaa aagtagggtt gtggaatgaa cgggattcat
     1441 taacagtaat gtatcggttt aaaaaaagag atgcacactt tgctgagtaa cacaacaagt
     1501 atgtttgatg ttgccgattt gcttgagtca ccacaaacaa ccattgcagt tttagctaca
     1561 ggagcacgta atgcagaact gctttatctt gttccttcac gtaaggaatt aaatattgat
     1621 gcattacaaa aggcattaac gggtcagctg agcgaaaaca aatcaagaac atttgccggt
     1681 gtcagcattt acgaacttca gtttagtaat tttagttcat catttacatt tgcttttgcc
     1741 aatggcattt ttattggaag ctttaacgca ggtttggttg aagatgctat caggcaacag
     1801 gcaacaggta agccctttgg tggtaatgtt tcggcacaaa agctgtacaa agaaatagct
     1861 gtttcatcag gaacaatact tggtctcaat tatcctgcat tgaaaaaact ttttagtgtt
     1921 gcaataagtt cagacaatgt tattgagtta aataatcttg aaaaatggac agaatggtct
     1981 tttcataatc tgacatttga tggcaattca ataaatgcca atggctcagt gcttgtatca
     2041 gattcaacac aacaaattgc agattttgga aatgtgcgtt cttcaactga tttgttgtgg
     2101 tcagtgctgc ctgatgatgt ttctgcatta cagatttttt cctttaaaga tgctctaatt
     2161 tggagtgata aaaatcaaag aaggaatgca gataatgaag taatcatcaa ctatataaag
     2221 caagtatctg taacagaaac aaagcttagg gtggaaataa gaaaagattt ttcgactata
     2281 gttggagaaa ttggagctat tgccattaat ggtaatccaa cttcattgct tgagaataat
     2341 tcagtggcaa tattgcagct aaaggatcag aaaaagtcat tgactcaatt actggcacta
     2401 agcaaaaagc tttcaaaaaa taaagacgtt attgctgtag agaaatttcg taatcatgaa
     2461 atacgctttt taaattgtga tggactctta ccggcaatgt ttggaaatgc ttttaatgtt
     2521 atcagaaaaa attattttac aactcataac ggttttgtaa tatttgctaa taagccaagt
     2581 cagttacgtg ggtttattga tgatgttgaa gataatgaaa tgcttaataa gaatgaagtg
     2641 tatcagcaga ataagtcagt tttatctgaa agcttgtctt atgctttatt cttaagacca
     2701 actaatctta attcatattt tcaatctgtt gcaaacagta atacattaaa agcattggcg
     2761 gcatcagatc tgatgtataa gttcagtagt ttttattatt acattcaacc gggtatttcc
     2821 gggaaagttg actgtaactt tagaattgtt atacagcaac aaaaacaaca aaaattgtct
     2881 gagccaaagt ggtcgttttt tacagattct gcaatacacn nnnnnnnnnn nnnnnnnnng
     2941 accatatatt tttaccagcg acaatggcat ttcaatcttg tttcaggatt caacaaaaag
     3001 tttaatctgt ctggatggca gtggtaatgt aagttggaaa acaactataa gcggatttat
     3061 taacggagaa atttctcaaa ctataaatga ccagtcaggt atgatacaat ttatttgtaa
     3121 tactaacgac agcatgtata tgtttaatca aaatggtgtt atgccggcac attatccgtt
     3181 taaattgcct gttcattcac gttatggtat tgtagcatca ccaaatacta atgttctttt
     3241 tgtagcaggt gagaataact taatgtatgc gtttgatccg ggcagcagac cttcacaaaa
     3301 ttggaataca gttcaatttg aaggcaactt atttaatgca tcaattgatg cattaggtag
     3361 gtttgtttac ataatggatg gcgataagtt aagactatat tcaacagatg gaaaactgat
     3421 tttatcgaag ccgtcaactg aatttagagt aaattcaatg caatgggtca atgactctgt
     3481 gccatcactc tttatgctga ataatgatgg aaaaattgtt gtttggaata tagatggtac
     3541 ttataaaatt gcatcaagcg acagtacttt tgattttgcg gaaacatgtg gttcaggtac
     3601 agcaagaatt tggtacacta ttaaaggtag ggaattgaat atattggatt acacattcaa
     3661 agcagttgaa aatgtagatt taagtaaagt tgagtttaac aacatgcaac tttttaatac
     3721 aggaaatgaa attttatgta tccttaaagg atctgaaa
//
LOCUS       DAOOOI010000154         3732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4339_length_3732_cov_16.983682, whole genome shotgun sequence.
ACCESSION   DAOOOI010000154 DAOOOI010000000
VERSION     DAOOOI010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4339_length_3732_cov_16.983682"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PKX59_11040"
     CDS             <1..837
                     /locus_tag="PKX59_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91434.1"
                     /translation="SITERCYKLPGVKFSQYIFLKNSGVVDLENDSIINFRLMLRDGV
                     GNISRCVFTVAYSNNAKPKNTVVKKGELLEYNKANKIQSDRYKLMLPAGALFRDFYCD
                     AIIRRGNKSFIAPQITIGNTSEGLKKNITLKIKSDLEGDSLNYKTIMVKIASNGTASP
                     VSGIYKDGWYSASISSFGTYSLMVDSISPVIENISTSFDSVYNCPVLEIKVSDNFSGI
                     KNYSCMINNKWHLSEYDRKNGTIYVLLNQFAESKFNIKIDLSDMCNNTTTLSKEIILS
                     KK"
     gene            complement(842..1717)
                     /locus_tag="PKX59_11045"
     CDS             complement(842..1717)
                     /locus_tag="PKX59_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ91435.1"
                     /translation="MKLQTIDTGFFKLDGGAMFGVVPKSIWQKLNPSDDNNMCTWALR
                     CLLIEDGNRLILIDNGIGDKQSQKFFSHYYLHGDSTLEKSLSKAGFHSDDITDVFLTH
                     LHFDHCGGSIKWNSSRDGFLPAFKNATYWCHADHWKWATEPNAREKASFLKENILPIK
                     DSGQLKFFEGTSDMSNGFEIIKKNNFHSDIDLVVARGHTDGMTLPLIPYKNGKLIFMA
                     DLLPSAAHVPLPYVMAYDTRPLVTLEEKKAFLKYAVHNKLNLFLEHDPTNECCNLLET
                     EKGVGVNSTFKLSEI"
     gene            complement(1698..2984)
                     /locus_tag="PKX59_11050"
     CDS             complement(1698..2984)
                     /locus_tag="PKX59_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91436.1"
                     /translation="MEVFLIPLVYILLICLLIGNHRFFELKGVSRAFVCILFIIKAFA
                     GIIITFNAEKIYSGDDGYFFYEAGKTFLDYGVENPYAYLKLMFGIYDSSNSEAYLHFL
                     NWKNLPMWNDGETMIKLCSIIHIFAFSNILVHSIVFSFLSFCGLTAIYKATIHITRSN
                     GFSLFLLLMLFPSVVFFSSGILKETLLIALSGLFFFSLTQFGVSLRMKIYCFILFFLL
                     WLIKPHFIFFFTPAIIAYITMAKLNLSSIWMSRLMILGVFALYIVLAVVVHDKVLGRN
                     AAGTIALVQQSSMKNSIYEKAESYIQPPVIAAKYSSILRNTPEAFYQVITVPWFYTNA
                     GLRWKAAAIENIFVLIMVLMNIYAAFDKKNKYALYHFAFLLFLISFYSLVGFTCALEA
                     SILRFKAPILPFLIAGMYMFLLQYKDKILKNETSDN"
     gene            complement(2986..>3732)
                     /locus_tag="PKX59_11055"
     CDS             complement(2986..>3732)
                     /locus_tag="PKX59_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91437.1"
                     /translation="SGLLKDSLILFATGMALYFLNRLIQERKSATIFGALFFLLFLMM
                     IKFHNFILLLPLFAAYLITSVYRKKTLLIFVSIVSVYYLILINLNLIMPGFGLMELLS
                     NKQAEFVDLANDYHANSTIAINYLAPSEWNAIVNTPQALLHSFFRPYPFESKNLFILL
                     AGFENLFFGLMMILVLLNFKIHKQAYPLFWVAVFYTLSLFELIGLVTPVMGAIVRYKI
                     QALPFLFFLLIYLTDIEKLGSRYMTKSKQT"
BASE COUNT         1394 a          660 c          570 g         1108 t
ORIGIN      
        1 agcattaccg aacgctgtta caaacttcct ggagtaaagt tcagtcagta tatttttctt
       61 aaaaatagtg gtgttgttga tttagagaat gacagtataa taaatttcag actaatgttg
      121 cgtgacggtg taggaaatat ttcacgctgt gtctttacag tagcttatag caacaatgcc
      181 aagcctaaaa atacagttgt aaaaaaagga gaattgttgg aatataataa ggcaaacaaa
      241 attcaatctg accgttataa actaatgtta cctgcaggag cattatttcg tgacttctat
      301 tgtgatgcca taattcgcag aggaaacaaa tcatttattg ctccgcaaat aacaattgga
      361 aacacatcag aaggattgaa aaagaatata actctaaaaa taaaatctga tcttgaaggc
      421 gattcgctca attacaaaac cataatggta aaaattgctt caaacggaac agcatcaccc
      481 gtatccggaa tttataaaga tggttggtat tcagcaagca tcagcagttt tggcacttac
      541 tcacttatgg ttgacagcat tagtcctgtc atagaaaata tttcaacttc attcgactca
      601 gtttataatt gtccggttct tgaaattaaa gtatcagaca atttttcggg aattaagaat
      661 tattcctgta tgattaacaa taaatggcac ttatctgaat atgacagaaa gaatggcact
      721 atctatgttt tattaaacca atttgcagag agtaaattta atattaaaat tgaccttagt
      781 gatatgtgca ataacacaac aacattgtca aaggaaatta ttttatctaa gaaataaaat
      841 cttaaatctc gcttagctta aaagtggaat ttacacccac acctttttct gtttccaaaa
      901 gattacaaca ctcattagtt gggtcatgct ctaaaaacaa attaagctta ttgtgcacag
      961 catatttcaa aaatgctttt ttctcttcta atgtaacaag tggccttgtg tcgtatgcca
     1021 taacgtaagg caatggaaca tgcgcagcag aaggtaataa atcagccata aatatcaatt
     1081 ttccattctt atatggaatc aaaggcaagg tcatgccatc agtatgaccc ctagcaacaa
     1141 caaggtctat gtctgaatgg aaattgttct ttttaattat ttcaaatccg tttgacatat
     1201 ctgatgtacc ttcaaaaaac tttaattgcc cgctatcttt tattggcaga atattttctt
     1261 ttaaaaagct tgccttctct cttgcatttg gttccgttgc ccacttccaa tggtctgcat
     1321 gacaccaata agttgcgttt ttaaatgcag gtaaaaatcc atctcttgat gaattccatt
     1381 taatacttcc tccgcaatgg tcgaaatgaa gatgtgtcaa aaatacatca gtgatatcat
     1441 cagaatgaaa acctgctttt gacaaggatt tttctaatgt gctgtcacca tgaagataat
     1501 agtgagaaaa aaacttttgg ctttgcttat cgccaatacc attgtcaatt aatatcagtc
     1561 tattcccatc ttcaatcaac agacatcgca atgcccatgt gcacatatta ttatcgtctg
     1621 atggatttaa tttttgccaa atagatttcg gcactacacc aaacatagca ccaccatcta
     1681 atttaaaaaa acctgtatca attgtctgaa gtttcattct ttaaaatttt atctttatac
     1741 tgaagtaaaa acatatacat acctgcaata agaaaaggga gtattggagc tttaaaccgt
     1801 aaaatagaag cttcaagtgc acaagtaaat ccaacaagac tataaaagga aataagaaac
     1861 aacagaaaag caaaatgata aagagcatat ttattctttt tatcaaatgc agcataaata
     1921 ttcatcaaca ccataataag cacaaagata ttttctattg cagcagcttt ccaccgtaaa
     1981 cctgcattag tataaaacca gggcactgtg ataacctgat aaaaagcttc gggtgtattc
     2041 ctaagaatac tactgtactt tgccgcaata accggaggtt gaatgtagct ttctgctttt
     2101 tcataaatag aattcttcat tgaactttgt tgtaccaagg ctattgtacc agcggcattt
     2161 cgtcctaaaa ctttatcatg cactaccaca gcaagtacaa tgtacaatgc gaaaacacca
     2221 agtatcatca atcgcgacat ccatattgaa ctcaaattca actttgccat agtgatatat
     2281 gcgataattg ccggggtaaa aaagaaaata aaatgaggtt ttatcagcca aagtaaaaag
     2341 aacagaataa aacaataaat tttcattcgc aaagacactc caaactgtgt aagactgaaa
     2401 aagaacaaac ctgataatgc aatcaatagt gtttctttta aaattcctga actaaaaaaa
     2461 actacagaag gaaaaagcat aagcagcaga aaaagactaa agccgtttga tcgtgttata
     2521 tgaatggtgg ctttataaat tgctgtcagt ccacaaaaag ataaaaagct aaaaacaata
     2581 gagtggacca gaatatttga aaaagcaaaa atgtgtatga tggaacacaa cttaatcatg
     2641 gtttctccat cattccacat tggcagattt ttccaattca ggaaatgaag ataggcctcg
     2701 ctatttgaac tatcataaat cccaaacatc agcttcaaat aggcataagg attttcaact
     2761 ccatagtcaa gaaatgtttt acctgcttcg taaaaaaaat acccatcatc accactgtaa
     2821 attttttctg cattaaatgt gatgataata cctgcaaatg ctttaatgat aaaaagaatg
     2881 caaacaaaag cacgcgatac ccctttaagt tcaaagaatc tgtgattacc aattaaaagg
     2941 caaattaata agatgtaaac taaagggata agaaatactt ccatcttatg tttgcttaga
     3001 tttagtcatg tatctgcttc ctaatttttc aatatctgtc aaatatatca atagaaagaa
     3061 tagaaatggc aaggcttgaa ttttatatct gacaattgca cccataacag gtgttactaa
     3121 gcctattagc tcaaacaaag aaagtgtata aaacactgca acccaaaaca atggatatgc
     3181 ctgcttatgt attttaaaat tcaataatac caaaatcatc atgagtccaa aaaataaatt
     3241 ttcaaatcct gccaacagaa tgaataagtt tttactctca aatggatagg gtctgaaaaa
     3301 agagtgtagc aatgcttgcg gtgtatttac aattgcattc cactctgatg gtgctaaata
     3361 atttatagct atagtactgt ttgcatgata atcatttgcc aagtcaacaa attcggcctg
     3421 cttgtttgac aacaattcca tcaaaccaaa gcccggcata attaaattaa gattaattaa
     3481 tatcaaataa taaactgaaa caatcgaaac aaaaatcaac aaagtctttt ttctgtaaac
     3541 tgaagtaata agatatgctg caaagagtgg caacagcaat ataaaattgt gaaatttaat
     3601 catcataaga aaaagtaaaa aaaacaaagc accaaaaatt gttgcagatt ttctctcctg
     3661 aatcaaccga ttcaaaaagt aaagagccat tcctgttgca aacaatatca gtgaatcttt
     3721 caacaatcca ga
//
LOCUS       DAOOOI010000155         3714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4364_length_3714_cov_6.808144, whole genome shotgun sequence.
ACCESSION   DAOOOI010000155 DAOOOI010000000
VERSION     DAOOOI010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4364_length_3714_cov_6.808144"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PKX59_11060"
     CDS             <1..780
                     /locus_tag="PKX59_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HOZ91438.1"
                     /translation="YVRLAFAVAAHLEPEILIVDEVLAVGDAEFQKKCMGKMKDVSGQ
                     GRTVLFVSHNIAAIKSLCTKGLYLQNGQVLKSGLIEPVITEYLAENKKIAETGDIPDD
                     FKRVYGNGDARFRKVYITDYQNNVMKQLPFGEKFKIHLEFDAIKDLTESTVSVMIGTT
                     SGENFMYSTDNNYSIHESKNFAKGKHHIEIEVDAKLLPGNFYITAGISISKTGFTLDW
                     VENVYMISVDKIGLNKGNDYPWDTVHGYVEPATNWKYKFLT"
     gene            787..1773
                     /locus_tag="PKX59_11065"
     CDS             787..1773
                     /locus_tag="PKX59_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOZ91439.1"
                     /translation="MELKGKKILVIGGAGFIGGFVVAELLKEDVKEVIIYDNFARGKM
                     ENIKESLKDARCSIYPMGGDVRETDILNDAMAGCDYVFHLAAMWLLHCKDYPRTAFDV
                     NIAGTFNVLEACVKNKIKKLIYSSSASVYGDAVQVPMTEDHPFNNKNFYGSTKIAGEA
                     MCTAYNDRYGLEVIGLRYMNVYGPGQDQHAVYSGVIPIMLNKIDANEAPSINGDGSQA
                     YDFIYVEDVARCNVAALKSETKYGFYNVGTEIQTTIKTLCDTILDLKKSDLKVKYVPY
                     SADDARALVQNRIGSAKKAKEEIGFEYKYNLVQGLSKLIEWRNKTIKSAIAV"
     gene            1854..3012
                     /locus_tag="PKX59_11070"
                     /pseudo
     CDS             1854..3012
                     /locus_tag="PKX59_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155544.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
     gene            complement(3057..>3465)
                     /locus_tag="PKX59_11075"
     CDS             complement(3057..>3465)
                     /locus_tag="PKX59_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOZ91440.1"
                     /translation="SAGKLVLSPTRTYAPVLKKVLEKHRAHIHGLVHCSGGGQTKVLH
                     FVEGLHVIKDNLFKVPPLFQLIQEQSQTPYSEMYKVFNMGHRMEIYVSAEHVQEIISI
                     ANTFQMEAQIIGRVEKSTTKKLTISSEFGTFEY"
     assembly_gap    3466..3498
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3499..>3714)
                     /locus_tag="PKX59_11080"
     CDS             complement(<3499..>3714)
                     /locus_tag="PKX59_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOZ91441.1"
                     /translation="TIQPGDVIIGLASSGKATYESEYNGGMGSNGLTSARHDVFENKY
                     AGLYPESYDTSIPADLAYSGKMNLQDE"
BASE COUNT         1216 a          689 c          770 g         1006 t
ORIGIN      
        1 tatgtgcgct tggcatttgc agtagcagcg cacttggaac ccgaaatttt aattgttgac
       61 gaagtgcttg cagtaggaga tgcagagttt caaaaaaaat gcatgggcaa aatgaaggat
      121 gtttccggac aaggcagaac ggttttattt gtaagccaca acattgccgc aattaaatcg
      181 ctttgcacca aaggcttgta ccttcaaaat ggacaagttt taaaatcagg tttaattgag
      241 ccggtaatta ctgaatacct agctgaaaat aaaaaaattg ctgaaacagg cgacattcct
      301 gacgatttta aacgcgtgta tggcaatggt gatgcacgtt ttcgtaaagt ttacatcacc
      361 gattatcaaa acaatgtgat gaaacaactg ccttttggtg aaaaatttaa aatacattta
      421 gagtttgatg ccataaaaga cttaactgaa tcaacagtta gtgtgatgat tggtactact
      481 tctggagaaa attttatgta ttcaaccgat aacaattaca gcatacatga aagtaaaaat
      541 tttgctaaag gaaaacacca catcgaaatt gaagtggatg caaaactttt gcccggaaac
      601 ttttacatca cagccggcat ttccatttct aaaacaggat tcacacttga ttgggtagaa
      661 aatgtgtaca tgatttctgt tgacaaaata ggattaaaca aaggaaacga ctatccttgg
      721 gatacggtgc acggatatgt ggagccggca acaaactgga aatataaatt tttaacatag
      781 aataaaatgg aacttaaagg aaaaaagatt ttagttattg gaggtgccgg atttattgga
      841 ggatttgtgg ttgccgaatt gctgaaagaa gatgtaaaag aagtaatcat atacgacaat
      901 tttgcgcgtg gcaaaatgga aaacattaaa gaatccttaa aagatgcgcg ctgcagtatt
      961 taccctatgg gaggtgatgt gcgcgaaacc gatattttaa acgatgccat ggccggttgt
     1021 gattatgtat ttcatttggc agccatgtgg ttgttgcatt gcaaagatta tccgcgaacg
     1081 gcctttgatg tgaatattgc cggaacattt aatgtgttag aagcctgtgt aaaaaataaa
     1141 atcaaaaaat taatttactc ttcctctgcc tctgtttatg gggatgcagt tcaagtacca
     1201 atgacagaag accatccatt taacaacaaa aatttttacg gctcaacaaa aattgcagga
     1261 gaagccatgt gcacagctta caacgaccgt tacggcttag aggtgattgg cttgcgttac
     1321 atgaatgtgt atggtcccgg gcaagaccaa catgccgttt acagtggagt aattccaata
     1381 atgctgaata aaattgatgc caacgaagct ccttccatta acggagatgg gtcacaagcc
     1441 tatgatttta tttatgtgga agatgttgca agatgcaatg tggcagcact aaagagtgaa
     1501 acaaaatatg gtttctacaa tgtggggact gaaatacaaa caacaataaa aacactttgt
     1561 gataccattc ttgatttaaa gaaatcagat ttaaaagtaa agtatgtacc ctatagcgct
     1621 gacgatgcac gtgccttggt acaaaataga attggttcgg ccaaaaaagc aaaagaagaa
     1681 attggatttg aatacaaata caatttggtg caaggattat ctaaattaat tgagtggcga
     1741 aataaaacaa taaaatctgc aattgcagtc tagccataaa ttctagtaca aaattcagca
     1801 caagcaatct aaacagattt gcaagcaata aaaatcacat cacacttcaa gaaatggaaa
     1861 ataaaacgat agaaaaaaga aacatcgcga tatccttgcc taccacaggt caggaagaat
     1921 gggaagcctt aaaagggcca atctttagtg gctggctaac gcaaggtcct cgtgttgccg
     1981 aaatggaaaa aatatttgca gaacgtcatc aggtaaaaca tgccattgca gtgagcaatt
     2041 gcacaactgc cttgcacctt gctttggttg cactgggtat aaagtccggt gacgaagttc
     2101 ttgtgcccgc atttacttgg gtaagtactg ccaatgccat catgtattgt ggcgccactc
     2161 ctgtgtttgt agatgtagat ccaattacct ttaatgtgga cgtgaaagac ctctcgcaaa
     2221 agattacttc caaaacaaaa gccattattc cggtgcatct ctttggtttg tgtgctgata
     2281 ttgacgaaat aaaacgaatt gctccacaac ttaaaattgt ggaagatggc gcttgcgcag
     2341 caggtgcagg atataaaaat aggccggcag gaagtttagg agacattggg tgcttttctt
     2401 ttcatccccg taaatcggtt accaccggcg aaggaggtat gctcaccaca aatgatgatg
     2461 ctctggcaga acacctcaac atgcttagaa atcacggtgc aagcattagc gaagaacaac
     2521 gtcacaaagg cccaaaacca tatattttac cggagtttga tatggtggga tacaattacc
     2581 gcatgaccga tttgcagggt gctgttggcg tggttcaact caaaaaactt gaccaataca
     2641 ttgacgagcg tcaacagtgg gctgattttt acacaaaaga attggcatct ataaaatggt
     2701 tgcgcacacc gcaagtgggt gccgactaca aacatggctg gcagtcgtac gtgctcctca
     2761 ttgacgaaaa aatttcacct ataaaacgaa atgatttaat ggaagtattg cagcaaaagg
     2821 gaatcgcaac gcgccccgga acacatgccg tgcacatgtt aaaattttta tgccagcaca
     2881 tttggtataa aaccttccga ctttccggga gcctttgctt gcaatgaata ttccatgagc
     2941 attcccttgc acaatagaat ggtaaaagaa gattacgaat atatagttgc agcgctaaaa
     3001 gaaattcgct gatttttaaa tcaaaaaata tagattcaaa acaaagggca taacttttag
     3061 tactcaaatg ttccaaactc actagaaata gtgagttttt ttgttgtgct tttttcaaca
     3121 cggccaatta tctgagcttc catttgaaaa gtgtttgcaa tggaaattat ttcttgaaca
     3181 tgctcggctg aaacataaat ttccattcgg tgtcccatgt taaacacttt atacatttca
     3241 ctataaggag tttgcgactg ctcttgaatc aactgaaaca agggtggtac tttaaaaaga
     3301 ttatccttaa tcacgtgtag cccttccaca aaatgaagta ccttggtttg accacctcca
     3361 ctgcaatgca caaggccatg tatatgcgct cggtgtttct ccaagacttt tttgagcacg
     3421 ggtgcataag tccgggtggg agataacaca agctttcctg ccgaannnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnntc tcatcctgta aattcatctt tccggaatag gccaaatcag
     3541 caggaataga agtatcataa ctttcaggat aaaggccggc atacttgttt tcaaaaacat
     3601 cgtgccgtgc acttgttagg ccattactgc ccattcctcc gttgtattcg gattcataag
     3661 ttgcctttcc ggaagaagcc aaaccaataa tcacgtctcc cggttgtata gtat
//
LOCUS       DAOOOI010000156         3708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4377_length_3708_cov_12.157952, whole genome shotgun sequence.
ACCESSION   DAOOOI010000156 DAOOOI010000000
VERSION     DAOOOI010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4377_length_3708_cov_12.157952"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="PKX59_11085"
     CDS             <1..1046
                     /locus_tag="PKX59_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91442.1"
                     /translation="SEKMLPELYRNMESKSEKMIPYVNTIKDTPDSGLEGFLETPRYA
                     SGYTALFNTIGFISETHMLKPYSVRVKATITFMETLRQYAARNAAEILQKRVEAINFT
                     KATHEFPVQWQLDTLSKDSIRFDGYTAVYSTSKITGLQRLSYDKSRPYSKFIPYQNNF
                     KPQVVIKQPFAYIIPQCWSHVIERLQLNGVEMQTITADTIIEVECYKIGQYKTTSKPY
                     EGHYLHSNVTVEKQVRKVKFFKGDRLIKVNQLANRYIVETLEPQGTDAFFAWGFFDGI
                     LQQKEWFSDYVFEDIAQKMIQENPALMDSLNDAIKSDSTLKNNHYMQLNMLYRLSPYF
                     EKSYMIYPVYRLM"
     gene            complement(1122..2708)
                     /locus_tag="PKX59_11090"
     CDS             complement(1122..2708)
                     /locus_tag="PKX59_11090"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ91443.1"
                     /translation="MKKTVFFLSLMFAQAVHAQTLKEAIAMTDNEQYEISGDMFKSLL
                     QKEPANGTNWFYYGENYFKSDNLDSAAIAYSKGLQAEPGNPINLVGTGKIKLEKGLNS
                     EARADFDKALTMSGSKTSLVQSEVAESFIRTKNKDLSYAIKLLNNAIAVDSKNAELYI
                     LLGDAYSEQNNGTLAAENYNRALDLDKKAVKAIVQKGVLYKRSTNYEGAAAEFQSAIK
                     LNPDFAPSYRELAETYFKMRKLDEAKQSYRKYLELSKNNSMARMRYASFLFMSGDYAD
                     AQTEINQLSSIDSSNIGMLRLSSYNAFEMNDIPKAKMLMEKIMNTTIENQRTAMDYEY
                     NGKILLKEGNDSLAAENFEKSYQIDTTKTEILQDMGSMYMKLKKYSEAVNAYERRVNN
                     GKGLTSTDYFNLARAYYFNKDYTHADTMFAKVIELLPTWPNGILWRAQTNAQLDPDSK
                     EGKAKPYYEKYIEVALTDTANSDKYKTGLIESYKYLGSYYYLIDKKPDECRSYWKKVL
                     EIAPDDKQAKQVMDGLNQKK"
     gene            complement(2742..>3708)
                     /locus_tag="PKX59_11095"
     CDS             complement(2742..>3708)
                     /locus_tag="PKX59_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOZ91444.1"
                     /translation="KKNHLMKVKNIFLLLLSTAITLQLVSCGGDAFKPDLSDTPTSGN
                     LKIVSDESFEPLMNSQAETFMSLYKNAKIEISYKTEADAINYLMNDSVKVIIAGKDLT
                     PEQHQYFKDHKVLARSTKIAVDALALIINKENIDSLLTLSQFESIITGKTNNWNAINN
                     KNNLGEVAVVFDKSGSSNVRFLKEKFLHNGNFPANCYALNSNKEVMDYVINKKNAIGI
                     IGVSWISDSNDSTAMTFLNQIKVVALKNDTSTEYTDYYQPYQAYIALKQYPLTRDVFL
                     INRQSRNGLGTGFASFVAGDQGQRLVRLKGLLPATMPVRIVRTGN"
BASE COUNT         1094 a          658 c          649 g         1307 t
ORIGIN      
        1 tttctgaaaa aatgcttccg gaactatatc ggaacatgga aagtaagtct gaaaaaatga
       61 ttccttatgt taatacaatt aaggacactc ccgattcagg tttagaggga tttttggaaa
      121 cacctcgata tgccagtggc tatactgcat tgtttaatac tattggtttt atttctgaaa
      181 cgcacatgct caagccatat tctgtcagag taaaggcaac tattacattt atggaaacac
      241 ttcggcaata tgcagcccgt aatgcagcag aaattttaca aaaacgtgtt gaggcaatta
      301 attttacaaa ggcaactcat gaatttccag ttcaatggca acttgataca ctttcaaaag
      361 acagtatccg ttttgatggc tacactgctg tttacagtac aagtaaaatt acagggcttc
      421 aaagactgag ttatgataaa agcagacctt actcaaagtt tattccatat cagaataatt
      481 tcaaaccgca agtagtaatt aaacaacctt ttgcctatat cattccacaa tgttggagcc
      541 atgtgattga aaggttgcag ttgaatggtg tagaaatgca aaccataaca gcagatacta
      601 tcatagaagt tgagtgttat aaaatcggtc aatataaaac tacttcaaaa ccttatgaag
      661 gacattatct gcattcgaat gttacagtgg aaaagcaagt ccgtaaggtg aaatttttta
      721 aaggtgatag attgattaaa gttaatcaac ttgccaatcg ttatattgtt gaaactttgg
      781 aacctcaagg cacagatgca ttttttgcat ggggattttt tgatggtatc ttacaacaaa
      841 aagaatggtt tagcgattat gtttttgaag atattgctca aaagatgatt caggagaatc
      901 ctgcattgat ggatagtttg aatgatgcaa ttaaatcaga ttcaacttta aagaacaatc
      961 attacatgca gttgaatatg ctgtacagac tgtcaccgta ctttgagaaa tcatatatga
     1021 tttatcctgt gtatagattg atgtaattgg ttaaaacgac atcgaacagt aaattcacat
     1081 ttactgttcg attggtggcc aaatgttatt atcgaaataa cttacttctt ctgattcaat
     1141 ccgtccataa cctgctttgc ctgtttatcg tcaggagcaa tttcaagtac ttttttccag
     1201 tatgatctgc actcatcagg ttttttatct atcagataat agtaactacc aagatattta
     1261 tacgactcaa taagtccggt tttatattta tctgaattgg cagtatctgt tagagctact
     1321 tcaatgtatt tctcatagta aggttttgct ttaccttctt tagagtcggg gtcgagttgc
     1381 gcattggttt gtgcgcgcca taaaatacca ttaggccatg ttggcaacag ttcaataact
     1441 tttgcaaaca tggtatctgc atgtgtatag tctttgttaa aataatatgc tcttgcaagg
     1501 ttaaagtagt cggtagaagt taagccttta ccattgttta ctcttcgctc gtaggcatta
     1561 acagcttcgc tgtatttttt taatttcata tacatactac ccatgtcctg aagaatttct
     1621 gtcttggtag tgtcaatctg atatgacttt tcaaaatttt cagcagctaa tgagtcatta
     1681 ccttctttaa gtaatatttt tccattgtat tcatagtcca ttgcagtgcg ttggttttct
     1741 atagtagtgt tcatgatttt ttccatcagc atttttgctt ttggaatatc attcatctca
     1801 aatgcattat atgaggacag acgaagcata ccaatattcg atgaatcaat tgaagacagt
     1861 tgattaattt ccgtttgtgc atctgcataa tcaccactca taaacagaaa ggatgcataa
     1921 cgcattcttg ccatactgtt attcttactc aactctaaat attttctgta ggattgtttt
     1981 gcctcatcca gcttgcgcat tttgaaataa gtttcagcaa gttcgcgata agatggtgca
     2041 aaatccgggt tcagttttat ggctgattga aattctgctg cagcaccttc atagtttgta
     2101 gagcgtttat acaatacccc tttttgaaca atagctttta cagctttttt atctaagtcc
     2161 aatgcacggt tgtaattttc tgcagccaat gtgccattgt tttgttcgga ataggcatcg
     2221 cctaaaagga tgtacaattc agcatttttt gaatcaacgg caatggcatt gtttagtaat
     2281 ttaattgcgt agctaaggtc tttatttttt gttctgataa aactttctgc gacttcgctt
     2341 tgcaccaacg atgttttaga gcctgacatt gttaatgctt tatcgaaatc ggcacgtgct
     2401 tcactgttaa gacctttctc taattttatt tttcctgtcc ctactaaatt tattggattg
     2461 ccgggttctg cctgcaatcc ttttgagtag gcaatagcag cagaatctaa gttgtcactt
     2521 ttaaaataat tttcgccata ataaaaccaa ttggtgccat tggcaggctc tttctgcaat
     2581 aatgatttaa acatgtcacc ggaaatttca tattgctcat tatctgtcat tgcaatagcc
     2641 tctttaagtg tttgtgcatg tacagcctgt gcgaacatca ggcttagaaa aaaaactgtt
     2701 tttttcattc tattaaaatt ggtaattata atttagttct gtcaatttcc tgttcttaca
     2761 attctaaccg gcatcgtagc tggcagcagt ccttttaatc ttacaagacg ctgcccctga
     2821 tcgcctgcaa caaatgatgc aaagccggtg ccaagtccgt ttcttgactg gcggttaatc
     2881 aaaaatacat ctctggttaa cggatattgc ttcaatgcta tatatgcctg ataaggttga
     2941 taataatctg tatattctgt tgatgtatca tttttaagtg caacaacttt aatctgattc
     3001 agaaaagtca ttgcagtgga atcattgctg tcgcttatcc aactaacacc aataatacca
     3061 attgcattct ttttattaat aacgtaatcc ataacctctt tgtttgagtt gagtgcataa
     3121 caattggcag gaaaatttcc attgtgtaaa aatttttctt tcagaaaacg tacattactt
     3181 gagccactct tgtcaaacac tacagctact tcacccaagt tatttttatt atttatagcg
     3241 ttccagttat ttgtttttcc tgttattatg gattcaaatt gtgataatgt cagcagtgaa
     3301 tctatgtttt ctttattgat aattaatgca agggcatcaa cagcaatttt agttgagcgt
     3361 gccagcactt tatgatcttt aaaatattga tgttgttccg gagtaaggtc ttttcctgca
     3421 ataataactt tcaccgagtc gttcatcaga taattaattg catctgcttc tgtcttatag
     3481 cttatttcaa ttttagcatt tttatagagt gacataaacg tttcagcctg actgttcatg
     3541 agaggctcga aactttcgtc agaaactatt tttaaatttc ctgatgtggg cgtatcactc
     3601 aaatcgggtt taaaggcatc tccaccacag gaaactaatt gcagcgtaat tgcagttgat
     3661 aggagtagga gaaaaatatt tttaactttc atcaagtggt tctttttc
//
LOCUS       DAOOOI010000157         3684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4401_length_3684_cov_16.722513, whole genome shotgun sequence.
ACCESSION   DAOOOI010000157 DAOOOI010000000
VERSION     DAOOOI010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4401_length_3684_cov_16.722513"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..923)
                     /locus_tag="PKX59_11100"
     CDS             complement(78..923)
                     /locus_tag="PKX59_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91445.1"
                     /translation="MKTNFKISDKLRSVLLIAGYVLVAATVLTLMSFATKRNHDLVCK
                     GVVVNIEDENVKGFIDRSDIMEIVRSKGKKIIGNKVTDINTFMLEKIIDANPHVLKAE
                     VYSTIDGWVHIDVQQRTPIVRIINSKGENFYIDNQGKFMPLSEKYSEPVMVASGQIAE
                     GLVQQKLGLDINSLPLPDDSVGKLPMLSQVFLLAEQLKRDSSWDAMFEQIYVDENGEI
                     ELIPRLGNNYILLGNISNLDEKLNLLLNLYRQGFPATGWDQYESINLKFKGQVVCKKK
                     NVKLS"
     gene            complement(920..2335)
                     /gene="murC"
                     /locus_tag="PKX59_11105"
     CDS             complement(920..2335)
                     /gene="murC"
                     /locus_tag="PKX59_11105"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOZ91446.1"
                     /translation="MKFENITHVYFIGIGGIGMSALARYFKAIGKQVAGYDKTQTALT
                     EELKGENIDVHFNDDVQAIPLAFMEANRKSNILVVYTPAIPKDHSELNFFSQQEFTVV
                     KRSQVLGVITSMHKTLAVAGTHGKTTTSSILAHILKTANINVAAFLGGIARNYNSNII
                     LPVSEHEVINVVEADEFDRSFLTLFPFAAAITSMDADHLDIYGSGEELKKTYQQFASQ
                     VDEKGLLVLRNGLQLQPVKALTKFYAADSQADYFADKISIRNHRYCFNFHSPDGIIED
                     LSLGLPGRHNVENAVAAIALAMKVGVKKESVRMALQSYKGVKRRFDIRFSDANCTFID
                     DYAHHPEELRAAIMSAHELFEGKKITGIFQPHLFSRTRDFADEFAASLALLDEIILLD
                     IYPARELPLEGITSSWLMNKIKNANKIFLNKTDVLDYINKREPEVLITLGAGDIDTLV
                     TPIQQLLEKKYSVHEFKNHKQ"
     gene            complement(2332..3480)
                     /gene="murG"
                     /locus_tag="PKX59_11110"
     CDS             complement(2332..3480)
                     /gene="murG"
                     /locus_tag="PKX59_11110"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020597095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOZ91447.1"
                     /translation="MQQPDIKVIISGGGTGGHIFPALAIANAIKSARPQAEILFVGAK
                     GRMEMEKVPAAGYKIEGLWISGIKRELTIDNLSFPFKVLSSISKAMHILKEFKPNAAV
                     GVGGYASGPLLYAASLKKIPCLIQEQNSYPGITNKILAKRVQKICVAYEGMDKYFPAS
                     KIILTGNPVRENVLMLDGKFDEAMSFFSFNPSQKTLLIVGGSQGARSINKSILAGLEK
                     LKAAGVQLIWQTGKLFYEEAQRAVAEVGYGQARVFDFIARMDFAYAAANLVVSRAGAS
                     TVSELTLTGKPSVMVPLPTAAEDHQTKNIEAMVKRDAAVLVKDTDALQHLADTALNII
                     NDADRLKQLSANAAAMALPHAAQKIADEVIAMATQHAAQNLKHVTAGS"
     gene            complement(3467..>3684)
                     /locus_tag="PKX59_11115"
     CDS             complement(3467..>3684)
                     /locus_tag="PKX59_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOZ91448.1"
                     /translation="AIGCAFSLVFQAMINMAVAVNLFPVTGQPLPMLSMGGTSIWFTS
                     IAVGIILSVSRNIEQEELKTGVVHAAA"
BASE COUNT         1079 a          795 c          653 g         1157 t
ORIGIN      
        1 gcaatttttg tagtgcctat atcaagacct actattactt cattacctgt tacctgctct
       61 gccacttatt gttagtttta tgatagttta acatttttct ttttgcatac tacctgacct
      121 ttaaatttca ggttgatgga ctcatactga tcccagcctg ttgccggaaa tccctgacgg
      181 tataaattga gaagaagatt cagtttctca tcaagatttg atatgttgcc aagcaaaata
      241 taattatttc ccagacgagg tatcaattca atttccccgt tctcatctac ataaatctgc
      301 tcaaacattg catcccacga tgaatcgcgc tttagttgtt ctgccaacaa aaaaacctgg
      361 ctcagcatag gtagttttcc tacagaatca tcaggcaaag gcaaggaatt tatatccaaa
      421 ccaagcttct gttgcactaa gccttcagca atctgaccgc ttgcaaccat cacaggctcg
      481 gaatattttt cagataatgg cataaatttt ccctgattgt ctatataaaa attttctcct
      541 tttgaattga ttatccgtac tatcggtgtt cgttgctgca cgtcaatatg tacccatcca
      601 tcaatggttg aatatacctc agcttttaaa acgtgagggt ttgcatcaat gattttttcc
      661 aacataaaag tgttaatatc agtcacctta ttaccaatga tttttttgcc tttggaacga
      721 acaatttcca tgatgtcgct gcggtcaata aaccccttta cattttcatc ttcgatgttc
      781 acaacaacac ctttacaaac aagatcgtgg tttcgttttg ttgcaaaact catcagagtt
      841 agaacagtgg cggcaaccaa aacatagccg gcaattaata aaactgaacg cagtttgtcg
      901 ctaatcttaa aattggtttt cattgtttat ggtttttaaa ttcgtgaact gaatattttt
      961 tttcaagcaa ttgctgaatg ggcgttacca acgtatcaat atcaccggca cctaaggtaa
     1021 tgagcacttc aggttcacgc ttattgatgt aatccaaaac atctgtcttg tttaagaata
     1081 ttttattagc atttttaatt ttattcatca gccatgacga agttattcct tccaacggta
     1141 attcgcgggc aggataaata tcaagaagta ttatttcatc aagtaaggcc agacttgcgg
     1201 caaactcatc agcaaaatca cgtgtacgtg aaaataaatg cggctgaaag atgcctgtaa
     1261 ttttttttcc ttcaaacaat tcgtgtgctg acatgatagc agcgcgcagt tcttcaggat
     1321 gatgtgcata gtcatcaatg aaagtacaat tagcatcaga gaaccggatg tcgaatctgc
     1381 gttttacacc tttataactt tgcagcgcca ttcgtaccga ttctttttta acaccaactt
     1441 tcattgcaag agcaatagct gcaacagcat tttcaacatt atgacggccg ggaagtccta
     1501 aagaaagatc ttcaataata ccatcaggcg aatgaaaatt aaaacaataa cgatgattac
     1561 ggattgaaat cttatctgca aaataatcgg cctgtgaatc tgcagcatag aacttcgtta
     1621 atgccttaac cggttgtagt tgcaatccgt ttcgtaaaac aagcaatcct ttttcgtcaa
     1681 cctgagaggc aaactgctga taggttttct ttaactcttc gccactgccg taaatatcaa
     1741 gatggtcggc atccatactt gtaatggcag cagcaaaagg aaataaggta agaaacgaac
     1801 ggtcaaattc atctgcttca accacattaa taacttcatg ttcactgaca ggaagaataa
     1861 tgttgctgtt ataatttctt gctattcccc caagaaaagc agccacatta atatttgctg
     1921 ttttaagaat atgggccaaa atacttgaag tagtggtctt gccatgtgtg cctgctacgg
     1981 caagtgtttt atgcattgaa gtgatgacac ctaaaacctg tgagcgtttt acaacagtaa
     2041 actcctgctg actgaaaaaa ttaagttctg aatgatcttt aggtattgca ggtgtgtaaa
     2101 caaccagaat attactcttt ctgtttgcct ccataaatgc taaaggtata gcttgtacat
     2161 catcattaaa atgcacgtct atattttcgc cttttaactc ctcagttaat gctgtttgag
     2221 ttttgtcata accggcaact tgtttgccga tagctttaaa ataacgtgca agcgcactca
     2281 ttccaatgcc gcctatgccg ataaaataaa catgtgttat attctcaaat ttcatgagcc
     2341 tgcagttaca tgtttaaggt tttgtgctgc atgttgcgta gccattgcaa taacttcgtc
     2401 agcaattttt tgtgctgcat gaggcagtgc cattgctgca gcgttggccg aaagttgttt
     2461 caaacgatcg gcatcattaa taatgttcaa tgctgtatca gctaagtgtt gcaatgcatc
     2521 ggtatctttt actaaaaccg ctgcgtctct tttcaccatg gcttcaatat tttttgtctg
     2581 atgatcttct gctgcagtag gcaatggaac cataacagaa ggtttacctg tcaaagtaag
     2641 ttcagaaact gtgcttgccc ctgcacgcga caccactaag tttgcagcag cataggcaaa
     2701 atccattctt gcaataaaat caaaaacacg tgcttgccca tagcctactt ccgcaacagc
     2761 acgctgtgcc tcttcataaa acagtttgcc cgtttgccat atcagttgca ctccagcagc
     2821 tttcagcttt tctaatccgg caagaatact tttattgata gagcgtgcgc cctgactgcc
     2881 acctacaatg agcaaagttt tttgtgaagg attaaaacta aaaaaagaca ttgcctcatc
     2941 aaactttcca tcaagcatca gcacattttc tctaaccgga tttccggtga gaattatttt
     3001 tgatgcaggg aaatatttat ccatgccttc ataggcaaca catattttct gaacacgttt
     3061 ggcaagaatt ttattggtga tacctggata ggaattctgc tcctgaatga gacaaggaat
     3121 tttttttaat gatgcagcgt aaagtaaagg tccgctggca taaccaccta cacctacagc
     3181 tgcatttggt ttaaactcct ttaaaatatg catagcctta ctgatgcttg acaacacctt
     3241 gaacggaaat gacaggttgt ctattgtcag ctctcgtttt attccgctta tccaaagtcc
     3301 ttcaatttta taaccggcag caggaacttt ttccatttcc attcggcctt tagcaccaac
     3361 aaaaagtatt tcagcctgag gtcttgcact tttaattgca ttggcaatgg caagtgcagg
     3421 aaaaatatgt cctcctgtac ctcctccgct gatgataact tttatgtcag gctgctgcat
     3481 gtactactcc tgtttttaac tcttcctgct caatattccg gctgacacta agaataatac
     3541 caacggcaat gctggtaaac caaatagatg ttccgcccat actcaacatc ggcaatggct
     3601 gccccgttac cggaaaaaga tttactgcaa ctgccatatt tatcatagcc tggaatacta
     3661 ggctgaatgc acagcctatt gcaa
//
LOCUS       DAOOOI010000158         3682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4410_length_3682_cov_8.672732, whole genome shotgun sequence.
ACCESSION   DAOOOI010000158 DAOOOI010000000
VERSION     DAOOOI010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4410_length_3682_cov_8.672732"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PKX59_11120"
     CDS             <1..633
                     /locus_tag="PKX59_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HOZ91449.1"
                     /translation="VYLQQGPFERPAKIDYKKPFYIYDTSVIHAFVTDSSGIESKWAK
                     AEYFKLPHPKWKVSIKGNYNPQYNAGGANGLIDGLHGDINWRKGRWQGYQSQDFECVV
                     DMGNQQEVSSFNTSFLQDTRAWILMPISVEYFTSDDGKLFNSAGIVQHQTAPDNYEIS
                     IENFKLQTEALITARYIKVVAKNFRKLPSWHQGFPMGGEAFIFVDEVEVE"
     gene            complement(642..1169)
                     /locus_tag="PKX59_11125"
     CDS             complement(642..1169)
                     /locus_tag="PKX59_11125"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOZ91450.1"
                     /translation="MATKRIDLKAFRIKSGRNRKKYTTFLKGLHSRKVKQLTAKAKKL
                     NDLAFKKIDCLACGNCCKTMTPTWQPADIKRVAAHFKMTVQQFKDKWLYKDETGDWMN
                     TKTPCQFLQRDNKCSIYEIRPKDCSGFPHLQHTDFLIGREVNIPNMTSCPITYEVVER
                     MYKLVVEKDATVLND"
     gene            complement(1253..2977)
                     /gene="lysS"
                     /locus_tag="PKX59_11130"
     CDS             complement(1253..2977)
                     /gene="lysS"
                     /locus_tag="PKX59_11130"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOZ91451.1"
                     /translation="MALSEQEQIRREKLNELLQMGINPYPAEKFEINTNSTFILENYE
                     QAEARFGAGKEEVSIGGRIMSVRDMGKAAFAQIQDAEGKIQIYIKRDEICKGDDKTLY
                     DTVWKKLIDIGDIIGIKGYVFKTKTGETSIHASSFILLCKSLRPLPIVKEKDGEVFDA
                     VIDPDLRYRQRYVDLIVNPQVKETFRKRTRLVNTMRNYLNEKGYLEVETPILQPLYGG
                     AAARPFKTHHNTLDMTLYLRIANELYLKRLIVGGYDGVYEFSKDFRNEGMSRFHNPEF
                     TQIELYVAYKDYEWMMNLVEEMIEKVALELHGSTAVKVGEKTIDFKRPWKRFTMYEAI
                     EHFTGVDVSEMDEMAMRKAAAQMGIELDDTMARGKIIDEIFGSKCEANLIQPTFITDY
                     PVEMSPLAKKHRSKPGLVERFEAICNGKEMCNAFSELNDPIDQRKRFEEQLELGKRGD
                     EEAMQLDEDFLRAIEYGMPPTAGLGIGIDRLSMIMTNAASIQDVLFFPQMKPETIETN
                     AATDTGLSHIPAPWNDVISRMGITSKQQLKEANPNKLFNDMGGMRKKLKLDISMVTLE
                     EVKSWINV"
     gene            complement(3270..>3682)
                     /gene="tsf"
                     /locus_tag="PKX59_11135"
     CDS             complement(3270..>3682)
                     /gene="tsf"
                     /locus_tag="PKX59_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOZ91452.1"
                     /translation="ITRYEVIEAPKVVVYIHPGNRVVSMVGLSSATAANEAGKDVAMQ
                     IAAMNPVAIDKDDVDQSTLDREIEIGKEQARAEGKPENMIEKIAMGKLNKFYQESTLL
                     NQAFIKEDKKTIRQYLDGVEKGLTVKAFRRVQIG"
BASE COUNT         1025 a          752 c          617 g         1288 t
ORIGIN      
        1 gtttatctgc aacaaggacc atttgaacgt ccggcaaaga ttgattataa aaaaccattt
       61 tatatttatg atacgtcagt aattcatgct tttgttacag actcaagcgg tattgaaagt
      121 aagtgggcaa aagctgagta ctttaaactt cctcatccaa aatggaaagt ttctattaaa
      181 ggaaattaca atccacaata taatgcaggc ggtgcaaacg gattgattga tggactgcat
      241 ggtgatataa actggcgtaa aggtcgctgg caaggttatc agtcgcagga ttttgaatgt
      301 gttgttgata tgggtaatca gcaagaagtt tcttctttta acaccagttt tttgcaagat
      361 acaagagcct ggattcttat gcctatttca gtggagtact ttacatctga tgatggtaag
      421 ttatttaata gtgcaggtat tgttcagcat cagactgcac cggataatta tgaaataagt
      481 attgagaatt ttaaattaca aaccgaagct ctaataacag cacgttacat aaaagttgtt
      541 gctaaaaatt tcagaaaact accttcatgg catcagggtt ttccaatggg tggcgaagct
      601 tttatttttg ttgatgaagt agaagtagaa tgataaagta cttagtcgtt cagtaccgtt
      661 gcatctttct ctacaactaa tttatacata cgctctacaa cttcataggt aatagggcaa
      721 ctggtcatat ttggaatatt tacttcgcga ccaattaaaa agtctgtgtg ttgcagatgt
      781 ggaaatccgg aacagtcctt aggacgaatt tcataaatac tgcacttatt atctctttgt
      841 aaaaactgac atggtgtttt tgtattcatc cagtcgccag tttcatcttt atacagccac
      901 ttgtctttaa actgttgcac tgtcatttta aaatgtgctg caactctttt tatatctgca
      961 ggttgccatg ttggtgtcat agtcttacaa caattaccac aggcaagaca atcaattttt
     1021 ttaaaagcaa gatcattaag tttctttgct tttgctgtca actgctttac cttgcggcta
     1081 tgcagtcctt tcagaaaagt tgtgtacttt tttctgtttc tcccagattt aattcggaat
     1141 gcttttaaat caattctttt tgttgccata gtttataaac aagcatgcaa atgaacaaat
     1201 ttattttata acttaattga tggtgtattg aagatattac ttcaggatgc ttttaaacat
     1261 taatccagct tttcacttct tcaagtgtaa ccatgcttat atcaagtttc agttttttac
     1321 gcatgccacc catgtcgtta aacaatttgt taggattagc ttctttgagc tgttgttttg
     1381 aagtgattcc cattctggaa ataacatcat tccaaggcgc aggtatgtgc gacaatcctg
     1441 tatccgttgc tgcattggtt tcaatagttt cgggcttcat ctgtgggaaa aacagcacat
     1501 cctgaatgct ggcagcgttt gtcataatca tactcaaacg atcaattcct attcctaaac
     1561 ctgctgttgg cggcatacca tattcaatgg cgcgaagaaa atcttcgtca agttgcattg
     1621 cttcctcatc gccacgtttg ccaagttcaa gttgttcttc aaatctttta cgctgatcaa
     1681 tcggatcatt caattcagaa aatgcattgc acatttcttt accattacat attgcttcaa
     1741 agcgttcgac caatccgggt ttgctacgat gcttctttgc caatggcgac atttcaacag
     1801 gatagtctgt aataaacgta ggttgaatga ggttggcttc gcacttgctt ccaaaaattt
     1861 catcaataat ttttcctctt gccattgtat catccaactc aattcccatt tgtgctgctg
     1921 ctttgcgcat agccatttca tccatttcac tgacatctac acctgtaaaa tgctcaatag
     1981 cttcatacat ggtaaatcgt ttccatgggc gtttaaaatc aattgtcttc tctcctactt
     2041 tcacagcagt tgagccatgc aattctaaag ctactttctc aatcatttcc tccaccaggt
     2101 tcatcatcca ctcgtaatct ttataagcta cataaagttc tatctgtgta aactccggat
     2161 tatgaaacct gctcatacct tcgtttctga aatctttcga aaactcatac acaccatcat
     2221 agccaccaac aattaatctt ttcagataaa gttcgttggc aatgcgcaaa tacaatgtca
     2281 tgtccaatgt attgtgatgc gttttaaatg gacgcgctgc agcaccacca tataaaggtt
     2341 gtaaaattgg tgtttctact tcgagataac ctttttcgtt caggtaattg cgcatggtat
     2401 tcaccaatcg tgtacgttta cggaatgttt cttttacctg cggatttaca attaaatcaa
     2461 cataacgctg acggtatctt aaatcaggat ctataacggc atcaaaaact tctccgtctt
     2521 tttcttttac aataggtaat ggacgaagtg acttacacaa cagtataaag gaacttgcat
     2581 gtatagaagt ttctcctgtt ttagttttaa aaacgtagcc tttaatgcca ataatatcac
     2641 ctatatcaat aagttttttc caaacagtat catacaaagt tttatcatca cctttacaaa
     2701 tttcatcacg ttttatgtat atctgaattt ttccttctgc atcctgtatc tgtgcaaacg
     2761 cagccttacc catatctctt acactcatga tgcggccacc aatactcacc tcttcttttc
     2821 ctgcaccaaa acgtgcttct gcctgttcgt agttttcaag aataaaggtg ctgtttgtgt
     2881 ttatttcaaa cttctctgcc ggatagggat taattcccat ctgtaagagc tcattcagct
     2941 tttctcgtct aatttgttct tgctctgata acgccattgt ttttaaattt gattgcaaag
     3001 atacatgttg ccgcttgcaa ttatcccttt cggtctgaca gtagtttttc ctgcttaggt
     3061 gctcacaatt tcccgatgag gacaaactat ttgtcggcac agcaatgcat atctattttt
     3121 gactcattga ttaaagatta ttctctgtta aaatattttt agttcagggt gggtttaact
     3181 tcccccgtca gcaaaactga cggggttttt ttatgtctcc ataaaaaaag ccaccctttt
     3241 ggagtggcct ttatattgtt ttggtttaat taaccaatct gaacacgtct gaaagctttt
     3301 acggtaagcc ctttttccac tccatccaaa tactgacgga tggttttttt atcttctttt
     3361 ataaaagctt gattcaacaa tgttgattcc tgataaaatt tattcagttt acccattgct
     3421 atcttctcaa tcatattctc cggtttgcct tctgcacgtg cctgctcttt accgatttct
     3481 atttcgcgat ctaatgtact ttgatccaca tcgtctttat caattgcaac aggattcatt
     3541 gcggcaattt gcatagccac atctttaccg gcttcattag ctgcagtagc agatgataaa
     3601 cctaccatag aaactactct gtttccgggg tgaatataaa ccactacttt aggagcttcg
     3661 attacttcat aacgagtaat tt
//
LOCUS       DAOOOI010000159         3643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4471_length_3643_cov_10.482163, whole genome shotgun sequence.
ACCESSION   DAOOOI010000159 DAOOOI010000000
VERSION     DAOOOI010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4471_length_3643_cov_10.482163"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2605)
                     /locus_tag="PKX59_11140"
     CDS             complement(<1..2605)
                     /locus_tag="PKX59_11140"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ91453.1"
                     /translation="MNRYFTTGRNITWKLCFAITLLLGTFNAKAQIPTASFAADSTEG
                     CVPLTVHFSNTSTLATSYFWDFGNGNTSTLANPQTVYLAPGNYTVKLIAYNSTSGQSD
                     TLIIPNYIDVVSFPTANFTSNVTSGCKPLSVVFNNTSSNFTSCIWDFGDGFSSTQTNP
                     THTYSNAGTYTVKLVAYSSYGCSNVKTQTNYITVYGQPTAQLTANPSSTCDANQVIQF
                     NSSSAGVVSYLWNFGQPSSGGANSSTLQDPTHTYGTQGSFNVSLIVTDANGCSDTAVI
                     NNMITIGTALTPTFTWTNGAGCKPLTTNFTAPSITGATSYAWDFGDPSSGVQNTSTSA
                     NPTHTYANSGTYSVSLYVTTSSGCNGGVTLNNIIVVDQPPVAAFTSNPTTGCKPLAVQ
                     FNNTSTGAATYLWEFGDGTTSTLASPSKIYTTAGTYNVTLHAFSTNGCETIIVKNNYI
                     VVNEIKSSYYFLPGSGCAPLSVNFIPTNNSGFVSYEWNFGDPASGALNTSTLSNPTHI
                     YNNIGTYSTKLIVTSAAGCKDTLIKNFVKVRASSVVYTVPDTIKGCQPYPVSFTNPLP
                     GATVFHWDFGVPWLTNDTSNIANPSFTFDSVGIFNVTLNAYMPSSGGAGCSQFFNPVA
                     IVQVFPMLISPITYIQATPCAPYLIHFSDTTMDVVSWDWDFGDGTPHATVQFPSHTYT
                     QPGTYTVVLNIVLQSGCMTSLSTTITLGYPNPITVNKKAGCHDEPFTFGLTGGPWTNV
                     QWNFGDGVGTSTSPNPTYTYNQVGTFTVSVNVTSSDGCVNTYTDSITTTNPQPSFVIN
                     GPTSSCTNQFVFFTNTSTGATSYWWDFGDPTSSTGFSSLVNPSRNFTTPKAYTISLTA
                     TNGACSRTVTYP"
     gene            complement(2657..3241)
                     /locus_tag="PKX59_11145"
     CDS             complement(2657..3241)
                     /locus_tag="PKX59_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91454.1"
                     /translation="MEDTTMNNTMDFYKMVRENRFAMYSKPRRYYNRDGIKGSAIKFL
                     FFVQAIIFFLMLVTLHSATAQTANTQPALMADANLNGTIANYTTIYNSGKVFLNWTAK
                     NEAADCLYIVERSLDGKVYESVGIKEGLGTAVELFYSWMDQTPPNGYAFYRVKKIARD
                     GSQYYSSTNTVINLDSSYDPKSNYASDKKPEDQK"
BASE COUNT         1120 a          609 c          723 g         1191 t
ORIGIN      
        1 ccgggtaagt aactgtacgt gaacaggcac cattggttgc agttagcgaa atggtatatg
       61 cttttggcgt agtaaaattt cttgatggat ttactagtga agaaaatcct gttgatgatg
      121 ttgggtcacc aaaatcccac caataagatg ttgcaccggt tgatgtgttt gtaaagaaca
      181 caaattgatt tgtacaactt gatgttggtc cgtttataac aaaagaaggt tgtgggtttg
      241 ttgtagtaat actatcagta taagtattaa cacatccatc actgcttgtt acatttacag
      301 aaaccgtgaa agtccctacc tgattataag tatatgttgg atttggagaa gttgatgtgc
      361 caacgccatc cccaaagttc cattgaacat ttgtccaagg gccacctgtt aatccgaatg
      421 taaatggttc atcatgacaa ccggcttttt tatttactgt tattggatta ggataaccta
      481 aagtgattgt tgtacttaaa ctggtcatac aaccactttg cagaacaata ttcagcacaa
      541 ctgtatatgt acccggttgt gtgtatgtat gcgaaggaaa ttgaactgta gcatgtggtg
      601 tgccatcgcc aaaatcccaa tcccatgaaa cgacatccat tgtggtatca ctaaagtgaa
      661 tcagatatgg tgcacatggt gtggcctgaa tataagtgat tggagaaatg agcattggaa
      721 aaacctgtac tatggcaaca ggattgaaaa attgtgagca acctgcacca ccactgctag
      781 gcatataagc atttaatgtt acattaaaaa ttcctacgga atcaaacgta aatgatggat
      841 tcgcaatgtt actagtgtca ttagttaacc acggaacacc aaaatcccaa tggaaaactg
      901 tagctccggg taatggattg gtaaatgata caggataagg ctgacaacct tttattgtgt
      961 caggaacggt atatactaca gatgatgctc ttacttttac aaagtttttt ataagcgtat
     1021 ctttacatcc ggcagctgat gttacaatca gtttagtaga gtatgttcca atgttattat
     1081 aaatatgtgt tggattagat aatgttgatg tgttcagtgc gcctgaagcc ggatcaccaa
     1141 aattccattc ataagaaaca aaaccactgt tatttgttgg tatgaaattg acacttaatg
     1201 gcgcacagcc tgagcctgga agaaaataat aactggattt aatttcatta acaacaatgt
     1261 agttgttttt tacaataatt gtttcacagc cattagtgct gaaggcatgt aatgttacat
     1321 tataagtacc ggcagttgta tatatcttcg atggtgatgc aagtgttgaa gtagtaccat
     1381 ctccaaattc ccaaaggtaa gtagccgcgc ctgttgaagt attgttaaat tgaactgcaa
     1441 gaggtttaca tcctgttgtt ggattagatg taaatgcagc taccggtggc tgatcaacta
     1501 caatgatatt atttaaggtt acacctccat tgcatccact tgatgttgta acatagagcg
     1561 aaacagaata tgttcctgag tttgcataag tatgtgtagg gtttgcagat gttgaagtgt
     1621 tttgaactcc tgatgaaggg tcaccaaagt cccatgcata actcgttgca cctgttattg
     1681 aaggcgcagt aaaatttgtt gttaacggct tacatcctgc accattagtc catgtaaatg
     1741 ttggagttag tgcagtacca attgtaatca tattgtttat aacagcagtg tctgaacacc
     1801 cattggcatc agtaacaatc aaactcacat taaaagaacc ttgagttcca taagtgtgtg
     1861 tagggtcctg aagtgttgaa gaattggcac caccggagga gggctgacca aagttccata
     1921 gataactcac aacaccggca gatgatgaat tgaattgaat aacctgattg gcatcacatg
     1981 tgctagatgg atttgctgtt aactgcgctg tcggttgacc gtaaacggtt atatagtttg
     2041 tctgtgtttt tacatttgaa caaccataag aagagtatgc aactaacttt actgtatatg
     2101 ttccggcatt gctgtatgta tgtgtcggat tggtttgtgt agatgaaaat ccatcaccaa
     2161 aatcccaaat acaagatgta aagttagatg atgtattgtt aaataccaca gatagtggtt
     2221 tacatcctga tgtaacgttg gatgtgaagt tagctgtcgg aaacgaaact acatcaatat
     2281 aatttggtat tatcaatgtg tctgattgtc ctgaagtact gttgtaggca atgagcttta
     2341 cagtataatt tccaggagct aaataaacag tttgcggatt ggctaatgtt gatgtattac
     2401 catttccaaa atcccagaaa taacttgtag ctaatgtgga tgtattgcta aagtgaaccg
     2461 tcaatggtac acatccttct gtagaatctg cagcaaatga tgctgttgga atctgtgctt
     2521 tagcattaaa cgtgcctagc agaagtgtaa ttgcaaaaca taacttccac gtgatgtttc
     2581 tgcctgtggt aaagtatctg ttcatatctg ataagttaaa aaaattcaaa gtgtaatgtc
     2641 gtcccttatt tattttttat ttctgatcct ccggcttttt gtccgaagca tagtttgatt
     2701 tcggatcata gctcgaatca agatttatta ccgtattggt agaactgtaa tattgcgagc
     2761 catcgcgagc aatctttttt actctgtaaa aagcatatcc gtttggcggt gtttgatcca
     2821 tccatgagta gaatagttct acagcagtac caagaccttc ttttatacct acggactcat
     2881 atacttttcc atcaagtgag cgctcaacga tataaagaca atcagccgct tcgtttttag
     2941 ccgtccagtt cagaaatact tttcctgaat tgtaaatggt tgtatagttt gcaatagttc
     3001 catttaagtt ggcatcagcc attaatgcag gttgtgtatt agctgtttgc gctgttgcag
     3061 aatgaagtgt tacaagcatc aaaaagaaaa tgatggcctg tacaaaaaac aagaacttaa
     3121 ttgctgaacc ttttatgcca tcgcgattat aatatctgcg tggttttgaa tacatggcaa
     3181 aacggttttc acgaaccatt ttgtaaaagt ccattgtgtt gttcatcgtt gtatcttcca
     3241 taatgatata tttttataag tgaaatttac gttttatatg agcagtggtt tttgtaattt
     3301 cttataaaac attacaatgt aatataactg cacattttct tttcatatcc gcttttacgc
     3361 ccattttgaa tccgggttac actttttcaa taaattgata ttattttgct tcataaaccg
     3421 tgctttttag tagtactttt attgttatac ataagctgat ttacagaaaa gtatatttag
     3481 tattacatat atgccatgga ttaatattga gtttcaatta tgcaattaga gtaattagct
     3541 aagtcggaaa ttttttaaaa aagctaaaac cttgcagttt tttttacgta acaaggaaga
     3601 gtctttaaaa aataaatcat aagcttatat ggatatatta aga
//
LOCUS       DAOOOI010000160         3621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4497_length_3621_cov_9.166854, whole genome shotgun sequence.
ACCESSION   DAOOOI010000160 DAOOOI010000000
VERSION     DAOOOI010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4497_length_3621_cov_9.166854"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>435
                     /locus_tag="PKX59_11150"
     CDS             <1..>435
                     /locus_tag="PKX59_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91455.1"
                     /translation="AQGMAVWHNIDEIGSRVILNPHGLEFYQALSVSDKLKAIPFKFV
                     FGYLFSKAAKVVSLGGRLTQLLRSNIKNHNNKIVVLPNAINLPAQKFERTSFDQRPLK
                     ILFVGRFAANKGIHVLMDAAAQLNNGGYKNELEFHLVGKGPL"
     assembly_gap    436..483
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <484..839
                     /locus_tag="PKX59_11155"
     CDS             <484..839
                     /locus_tag="PKX59_11155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOZ91456.1"
                     /translation="DDDKLNSLYKDCDVFVLPTLFEGMPTVVLEAMSYSLPIVVTDTG
                     ATTVMVDSLNGYIIEKSDVKSLKDAISMYFVLNEQAKKRMAEQSYSRVKEKFTWPVIA
                     QQHLELFQQMAEQKT"
     gene            869..2188
                     /locus_tag="PKX59_11160"
     CDS             869..2188
                     /locus_tag="PKX59_11160"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOZ91457.1"
                     /translation="MLQFIRNNAQFVIMCFTWVFCGMVNETLGVAAVVLSVIMLKIKG
                     MYKELILGLIFLLIMSDSRQFEALYAAQAKNFYIVLLSVFYFFDRKNFTGTNDYFKPF
                     IPFFLWSIAMLMQAPTVNLMNSAMKTLSYILLFTVVPAYFLKAFRELGESFLRDVIML
                     FCCILLAGIIMIPLFPNTVFLVGRFSGVYGNPNGIGIGCTLLFILVYISLKKFPGLLD
                     QNEVRLIYGLIILSTLLAVSRNTMMSIILFLLFARFYEMGYLAGFAILVVALVAYELI
                     NQNIVAIVEALGLGHYLRAEAIEDGSGRLIAWRAAWKQIQSQFFLGRGFAYDEWYFYA
                     NRHWLSALGHQGGVHNSWLALWMNTGLIGLILFAVGFIKTFVRAMHHSKLAFPILFAV
                     IFSASFEAWIMGSLNPFHIILILVLNLIIHYNQFNADREEESLVSVS"
     gene            2148..3218
                     /locus_tag="PKX59_11165"
     CDS             2148..3218
                     /locus_tag="PKX59_11165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOZ91458.1"
                     /translation="MPIAKKKALFLYLEPAGYVEACFKKTALNYPLDLHIVKYPLDPN
                     APFRFDDVESITYYDRNQYQEQKLNDLVASINPDFIFCNGWMDKGYVNVAKQYSGKIP
                     TVLAFDNLWEGKLKQYVASVTAPFILPRIFSHCWVPGQPQVIYAKKLGFKENRIETGM
                     YSANYELFDQAYQKIMPIKEKNFPKRFIFVGRYIEIKGIRELWQAFTQLQNEMPNEWE
                     LWCLGKGPLEKEFPQHPKIRNVGFVQPSAMEEYMRQTGVFLLPTYDEHWGVVVHEYAI
                     AGYPLLLSTKAPAGTAFLKQGENGYYHEPSNVDSLKEALKKIILLPDSTLIEMGKKSH
                     ELSKSMTPDTWAAVVWKILNSK"
     gene            3222..>3621
                     /locus_tag="PKX59_11170"
     CDS             3222..>3621
                     /locus_tag="PKX59_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015850286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOZ91459.1"
                     /translation="MCGITGIYGMSSEKSASTIRLMNNAISHRGPDDEGYYVDEQVAL
                     GHRRLAIIDLSPAGHQPMHSNDGNLEIAFNGEIYNFQEVKKLLSGYAFKSGSDTEVIL
                     AAYQQWGKDCLQHFNGMFAFAIWDKAKQELF"
BASE COUNT         1088 a          556 c          732 g         1197 t
ORIGIN      
        1 atgcacaggg aatggctgtg tggcataata ttgatgaaat tgggagccga gttattctaa
       61 acccacatgg attggagttt tatcaggcac tttccgtttc tgataaatta aaagcaatac
      121 catttaagtt tgttttcggc tatctttttt caaaggctgc aaaagttgtt tcgcttggtg
      181 gccgtttgac acagctttta agaagtaata ttaaaaatca taataataaa attgttgtat
      241 tacccaatgc aattaatttg cctgctcaaa aatttgagcg aacatcattt gatcaaaggc
      301 cgttaaaaat tttgtttgtt ggaagatttg ctgccaataa gggcattcat gtattgatgg
      361 atgcagcagc tcagttaaat aatggtgggt ataaaaatga acttgagttt catttggttg
      421 gcaaaggtcc tttgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnccgatga tgataagtta aacagccttt ataaagattg tgacgttttt gttttgccaa
      541 cattgtttga aggaatgcca acagtagtgt tagaagcaat gagttattct ttgccaatag
      601 ttgtaacaga tacaggtgca acaaccgtta tggttgattc tttgaatggc tatatcattg
      661 aaaaatctga tgtaaagtcg cttaaagatg ctatttcaat gtactttgtt ttgaacgaac
      721 aggcaaagaa acgaatggct gaacaatctt attcacgagt aaaagagaaa tttacctggc
      781 ctgttattgc acaacagcat cttgagctct ttcaacaaat ggctgaacag aaaacataaa
      841 tgttaatttt gtaattctaa accaccaaat gcttcagttt atcagaaata atgcacagtt
      901 tgtaatcatg tgtttcacat gggttttctg tggtatggta aatgagactc ttggtgttgc
      961 tgcagttgtg ttaagtgtta ttatgctcaa aataaagggc atgtacaaag aacttattct
     1021 tggattgata tttctgctta tcatgagtga tagcagacaa ttcgaagctt tatatgccgc
     1081 acaggcaaag aatttttata tcgttttgct ttcagtcttt tatttttttg acagaaaaaa
     1141 tttcacaggc acaaatgatt attttaaacc gttcattcca ttcttcttgt ggagtattgc
     1201 tatgctgatg caagcaccaa cggtaaacct tatgaatagc gctatgaaga cattaagtta
     1261 catactgctg tttactgttg ttccggcata ttttttaaaa gcttttaggg agcttggtga
     1321 aagttttttg cgtgacgtaa taatgctctt ttgttgcatt ttattggcag gaataattat
     1381 gattccactc ttcccaaaca cagtgtttct tgtgggtaga tttagtggtg tttatggcaa
     1441 tcccaatggt attggtattg gctgtacact gttgtttata ttagtttaca tttcattaaa
     1501 aaagtttccg ggccttcttg accagaatga agtcagactt atatacggcc taattatttt
     1561 aagtacgttg ttggcagttt cacgaaacac aatgatgtca atcattttgt tcttactttt
     1621 tgctcgcttc tatgagatgg gttaccttgc aggatttgca attctggtag tggcacttgt
     1681 tgcttatgaa ttaatcaatc agaatatagt tgctattgta gaagcattgg gtcttggaca
     1741 ttatttgcgt gcagaagcta ttgaagatgg ctcaggcaga ttaattgcat ggcgtgctgc
     1801 atggaaacaa attcaatcac aatttttttt aggtagaggc tttgcctatg atgaatggta
     1861 tttttatgca aacagacatt ggttaagtgc attaggtcat cagggagggg tacacaactc
     1921 atggcttgct ttatggatga acacgggttt gataggactt attctttttg cagtagggtt
     1981 tataaaaact tttgtcagag caatgcatca ttctaaattg gctttcccaa ttttatttgc
     2041 tgtaatattt tcagcttcgt tcgaagcctg gataatgggt tcattaaatc catttcatat
     2101 tattttaatt ttagttttga atttaatcat acattataat caatttaatg ccgatcgcga
     2161 agaagaaagc cttgtttctg tatcttgaac ctgccggtta tgttgaagct tgttttaaaa
     2221 agacagccct aaactatcca ttggatttac atattgtaaa atatccgctt gatcccaatg
     2281 cgccattccg ctttgatgat gtagaaagca ttacttatta cgacagaaat cagtatcagg
     2341 agcagaagct taatgacctt gttgcttcta taaatcctga ttttattttt tgtaatggat
     2401 ggatggataa aggttatgta aatgtcgcta aacaatattc cggaaaaata ccaacagtac
     2461 ttgccttcga taatttgtgg gaaggaaaac ttaaacagta tgtagcatcg gtaacagctc
     2521 catttatttt acctcgcatt ttttcacatt gctgggtgcc gggtcagccg caggttattt
     2581 atgcaaaaaa acttggcttt aaagaaaatc gtattgaaac aggaatgtat tctgccaatt
     2641 acgaattgtt tgatcaggca tatcaaaaaa taatgcctat aaaagaaaag aattttccta
     2701 agcgttttat ttttgttgga cgttatatcg aaataaaagg tattcgcgaa ctatggcagg
     2761 catttacgca gttgcagaat gaaatgccta atgaatggga gttgtggtgc cttggtaaag
     2821 gtccacttga aaaagaattt ccgcaacatc ctaaaatcag aaatgtagga tttgttcaac
     2881 cttctgctat ggaagagtat atgcgccaga caggtgtatt tcttttacca acttacgatg
     2941 agcattgggg tgtagtagta catgaatatg ccattgcagg ttatccgttg ttgttaagta
     3001 caaaagcgcc tgcaggaact gcatttttaa aacaaggtga gaacggatat taccatgagc
     3061 caagcaatgt tgattcgctc aaagaggcac taaaaaaaat aatactattg cccgatagta
     3121 ctttaataga gatgggtaaa aaaagtcatg aattgtcaaa gtctatgaca cccgatacgt
     3181 gggctgcagt agtctggaaa attttaaaca gtaaataaac tatgtgtgga ataacaggca
     3241 tttatggaat gagcagtgag aaatcggcat caacaataag gttgatgaat aatgctattt
     3301 cacaccgcgg ccctgatgat gaaggatact atgttgacga gcaagttgct cttggtcatc
     3361 gaagattagc aataattgat ctttctcctg caggccatca gccaatgcat agcaatgatg
     3421 gtaatcttga aattgctttc aatggtgaga tttataattt tcaggaagta aaaaaacttc
     3481 tttcaggata tgcgtttaaa tcaggcagtg atactgaagt aattcttgca gcctatcagc
     3541 aatggggaaa agattgtttg cagcatttta atggcatgtt tgcttttgcc atttgggata
     3601 aagccaaaca agaacttttt a
//
LOCUS       DAOOOI010000161         3586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4545_length_3586_cov_28.860096, whole genome shotgun sequence.
ACCESSION   DAOOOI010000161 DAOOOI010000000
VERSION     DAOOOI010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4545_length_3586_cov_28.860096"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..366)
                     /locus_tag="PKX59_11175"
     CDS             complement(151..366)
                     /locus_tag="PKX59_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91460.1"
                     /translation="MKNIEIRTQDKATKKTTYRINLSNVIVFLRHDEDKIIVRDDEQV
                     MVEVYHNGELIFSDSKIEFYNKLKNNV"
     gene            complement(363..899)
                     /locus_tag="PKX59_11180"
     CDS             complement(363..899)
                     /locus_tag="PKX59_11180"
                     /inference="COORDINATES: protein motif:HMM:NF037233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91461.1"
                     /translation="MANWCSNHVSVTGDNPNVQAFVNEIIELSLKYRKIEEGVLPEYC
                     EYLKDGYLFFDNEIYEKETTDFSADNPCFNYSTQWSPNLAILKCMAEHHKVNIESEYE
                     EMGMQIYGKWTYNDGIENDVWLEQEDFDLYTEYEENYTYTYKGEVYNSEWEVLELILE
                     NKIAELKKQHFLTNKETV"
     gene            complement(902..1066)
                     /locus_tag="PKX59_11185"
     CDS             complement(902..1066)
                     /locus_tag="PKX59_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91462.1"
                     /translation="MTKIIILDFNTGEAHIYTYDSAEFPEAEDFVNSEGFQSSNCQWL
                     TSDSLTLTIH"
     gene            complement(1084..1374)
                     /locus_tag="PKX59_11190"
     CDS             complement(1084..1374)
                     /locus_tag="PKX59_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91463.1"
                     /translation="MKKTIKPVDVTKIADFYKRQMLRETVNIIQNFGTMYVDTADNKE
                     HVYCLNKVQEMLSKIDEKEKQKFSKRSLNEIKILESVLAKNDCAYIRFIDIY"
     gene            complement(1385..1555)
                     /locus_tag="PKX59_11195"
     CDS             complement(1385..1555)
                     /locus_tag="PKX59_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91464.1"
                     /translation="MIAEDMFAEDMLQIFDKKTGAYIGLIKRGEPIPEIYTLDKTEQK
                     IEVIDVNQLSLF"
     gene            complement(1591..1776)
                     /locus_tag="PKX59_11200"
     CDS             complement(1591..1776)
                     /locus_tag="PKX59_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91465.1"
                     /translation="MQYNLNFLYESGEILVANVSGKKSQMLAVIKADNSIEYIKQNLP
                     TKVKNQIELEANNRVNY"
     gene            complement(1776..2909)
                     /locus_tag="PKX59_11205"
     CDS             complement(1776..2909)
                     /locus_tag="PKX59_11205"
                     /inference="COORDINATES: protein motif:HMM:NF037528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91466.1"
                     /translation="MKNLVATEMFIMGPGGGMEKLEPMKELPLYTKVRKYGAGMLDVD
                     GVVIEPANEHGAQKCVSISESYNVFFTIDRYSRPLSKKFGIGTYYYDDMSKVEKSEVL
                     KYIEKAIKADVERAEKKRIKAEADAKELAELPAKFPHLKAVTENADKKEVKDNLMADL
                     KKNFPGIRFSVTKSNYSTYQIRWTDGPTKKEVNAISDKFEAYSSDITGDFYDYTPSLF
                     NRVFGGFKFVFCNRDMSPEVMQVNKDLEVGSYQDKLDLVFNVWQKTSFPAKFTPTGIK
                     HKDDVMSGNDRADFYEITFDTPAPAEVENTEVMAGEIVFPNGVKIVDYSERAIALIGD
                     TKPIKDTLSGLGGRFNFRLTCGAGWIFPMTKKETVVNALNKLS"
     gene            complement(2933..3403)
                     /locus_tag="PKX59_11210"
     CDS             complement(2933..3403)
                     /locus_tag="PKX59_11210"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HOZ91467.1"
                     /translation="MQYLAEEPRTYLKNLPEFSIKYKKGQVKEVKIINAQTAYELLKE
                     LYDADTLEYCESSIVIFLNRNNRSIGWFKVSHGGISGTVVDNRMILATALTAGASSII
                     MSHNHPSGNINPSDIDDKLTSKLKQACDILDIKLLDHIIVTADDYYSYGEQGKI"
BASE COUNT          913 a          739 c          561 g         1373 t
ORIGIN      
        1 ctatacctgc atttttgcat tcattcctgt actcaaaata actatctaaa aaaactttcg
       61 acggagtaaa tccactattt gtatgcatta ttactgtctg cttggtgcct ttgatatgtt
      121 tactgttcca cttcatcatt ttatcgtaac tcatacatta ttttttagct tgttataaaa
      181 ttcaatctta ctgtctgaaa atatcaactc tccattgtgg taaacttcta ccattacctg
      241 ctcatcatcc cgaacgataa tcttgtcctc gtcgtgccgt aaaaatacta tcacgtttga
      301 cagattaatt ctgtacgtcg tctttttggt agctttgtcc tgtgttctga tttctatatt
      361 tttcatactg tttctttatt ggttaaaaaa tgttgtttct ttaattctgc aatcttattc
      421 tccaaaatca actccaatac ttcccattca gagttataca cttcaccttt gtatgtatag
      481 gtataattct cctcatactc ggtatatagg tcgaaatcct cctgctctaa ccatacgtca
      541 ttttcgatgc cgtcattgta ggtccacttt ccgtaaatct gcatacccat ttcctcgtac
      601 tcactttcaa tgtttacttt gtgatgttct gccatacact tcaggattgc caggttcggg
      661 ctccattggg tagaatagtt aaagcatgga ttatccgcgc tgaaatcagt cgtttctttc
      721 tcataaatct cgttatcaaa aaagagataa ccgtctttta aatattcgca gtattcaggc
      781 agtacaccct cctcaatttt tctgtacttc aggcttaatt caataatctc attaacgaat
      841 gcctgtacgt tcggattgtc accggtaacg cttacgtgat tgctacacca atttgccata
      901 attaatgaat tgttagtgtt aatgaatccg atgttagcca ttggcaatta cttgattgaa
      961 aaccctcgga gtttacgaaa tcttctgcct ctgggaactc tgcactgtca tacgtgtata
     1021 tgtgtgcttc acctgtgttg aagtctaaaa taataatctt tgtcatgctg ctaattttaa
     1081 ttgttaataa atatctataa atctgatgta ggcacaatcg ttctttgcca atacgctttc
     1141 gagtattttt atttcattca acgaacgttt gctaaatttc tgtttctctt tctcatcaat
     1201 tttgcttaac atttcctgta ctttattcag gcaatataca tgctctttat tatctgcagt
     1261 gtccacgtac atcgttccaa aattttgaat aatgtttact gtttctctta gcatctgacg
     1321 tttataaaag tctgctatct tagttacatc aactggtttt attgtctttt tcattactga
     1381 ttatttagaa taatgataat tgatttacgt ctataacttc tattttttgc tctgttttat
     1441 cgagcgtgta tatttcaggt ataggctcac cacgtttgat taaaccgatg tatgctcctg
     1501 ttttcttgtc gaatatttgg agcatatcct ccgcaaacat atcctctgca atcatgttcg
     1561 ttcgtcggtt cggtataatc ttttgcatgg ttaataattt actctgttat ttgcctctaa
     1621 ttcaatctga tttttcactt tagtaggtaa gttctgtttg atgtattcta tcgaattatc
     1681 tgctttaatt acggccaaca tttggctctt tttaccggat acgttggcta ctaatatttc
     1741 accgctttcg tatagaaaat ttagattgta ttgcattacg ataacttatt taaagcgtta
     1801 acaactgttt ctttttttgt cattggaaat atccaacctg ctccgcaagt taatctgaag
     1861 ttgaaacggc cacctaatcc actcaatgta tctttgatag gcttagtatc tccgattagt
     1921 gcaatggctc tttcagaata gtcaactatt ttcactccat tcggaaatac gatttcacct
     1981 gccataactt ctgtattttc cacttcagca ggtgccggag tatcaaaggt tatctcgtaa
     2041 aaatcagctc tgtcattacc gctcataaca tcgtctttat gtttgatgcc tgtaggagtg
     2101 aacttggcag ggaaactagt cttttgccat acgttaaata ccaaatccaa tttgtcctga
     2161 taactaccta cctccaaatc tttatttacc tgcattactt caggactcat atctctatta
     2221 cagaatacga atttgaatcc tccgaatact ctgttaaaca ggctcggtgt ataatcgtaa
     2281 aaatctcctg tgatgtcgct actgtatgcc tcaaacttgt cgctaattgc attcacttct
     2341 tttttggtcg gtccgtccgt ccacctgatt tgataagtgg agtaattcga tttcgttacc
     2401 gaaaatcgga tgccgggaaa gttcttttta agatcggcca taaggttatc tttcacctct
     2461 tttttgtcag cgttctctgt tactgctttc aggtgtggaa actttgcagg taattcggct
     2521 aattcctttg catctgcttc ggcttttata cgtttttttt cagcacgttc aacgtcggct
     2581 ttgattgctt tctcaatgta ctttaggact tctgattttt ctactttgct catgtcgtca
     2641 tagtagtaag tacctatacc aaatttcttg cttaatggac gtgaataacg gtctattgta
     2701 aaaaatacgt tataactttc agatatggaa acgcatttct gtgcgccatg ctcgtttgca
     2761 ggttctatga ctactccgtc cacgtccaac ataccggctc cgtattttct gactttcgtg
     2821 tataacggta actctttcat tggttccaat ttttccatgc caccccctgg gcccatgata
     2881 aacatttctg ttgctactaa atttttcatg ctattttttt tatttgattt gattaaattt
     2941 taccctgttc accataactg taataatcgt ctgcagtcac tataatatgg tctaataatt
     3001 tgatgtctaa tatatcacaa gcctgtttta gtttacttgt taatttgtcg tcaatatcgc
     3061 ttggattaat attaccgctc ggatgattat ggctcataat tattgatgat gctccagcgg
     3121 ttaatgccgt cgctaatatc attcggttat ctacaactgt acctgaaata cctccatgac
     3181 taactttgaa ccaaccgata cttctgttat ttctattcag gaaaattacg attgaactct
     3241 cacaatactc caatgtgtct gcgtcataca attcttttag cagttcatag gcagtctgtg
     3301 cgttaatgat ttttacttct tttacttgcc cttttttata ctttatagaa aactcaggca
     3361 gattctttaa atacgttctt ggttcctcag cgagatattg catggtacta atttttagat
     3421 gtgattaata acggcagaaa ctccgtcgta attatgaaat aatgcttctg cactaaagaa
     3481 taaaagtgct gcggttgcgt ccattgcatc aatgaaatac gtcttagcga ttccggatgc
     3541 tgttttgaag tttactttaa atgttctcat aattgctgtt ttttta
//
LOCUS       DAOOOI010000162         3514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4673_length_3514_cov_22.369471, whole genome shotgun sequence.
ACCESSION   DAOOOI010000162 DAOOOI010000000
VERSION     DAOOOI010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4673_length_3514_cov_22.369471"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..618)
                     /locus_tag="PKX59_11215"
     CDS             complement(106..618)
                     /locus_tag="PKX59_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOZ91468.1"
                     /translation="MNHLKKLVVVFSCAVSVSSAQVFESRPIPAVDVKDLNGKTVNTS
                     TLNNNGKPMVLDFWATWCKPCVNELTAISEHYEEWQKETGVKIVAISVDDARTMANVK
                     PFVNGKGWDYEVYVDPNGDLKRALNIGAVPYVFLVNGKGEIVSVKTGYAPGDEHKMYE
                     EIKALTKESN"
     gene            complement(639..1340)
                     /locus_tag="PKX59_11220"
     CDS             complement(639..1340)
                     /locus_tag="PKX59_11220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91469.1"
                     /translation="MKKIVLALILITSASLVKAQSFSYTGLSADVYGSVEDSLLHSYV
                     YVINNSQNTIDASLNAYDVYKVPGTEHNFCWGILCYGVGVMSSAIPATINAGATEGSF
                     RGDYLGHGYVGTTTVAYTVFDGNNPLDSVQFAINYHITPVGVKEVNKTNSISAPSPNP
                     ARSITAFNYNIKSGSKAEVKVINMLGKTVKNFNVNSSTGNIVLSTADLPSGVYFISLN
                     VEGKSSAVQKLVVNR"
     gene            complement(1398..3281)
                     /locus_tag="PKX59_11225"
     CDS             complement(1398..3281)
                     /locus_tag="PKX59_11225"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOZ91470.1"
                     /translation="MKKIFTILTAACLVVGIANAQSQRYVVVEEFTGETCGPCAANNP
                     GFNTKLNANAFTIPIKYQNNIPSNGPNYYSYNTIDVANRTSFYANNYSPHAFLDGNVW
                     DGNAGSFPVATLNSRVATTSPFTVDVTHSFSPTHDIIYTHTVIRATQAVTGLTSLKAR
                     IAITEKDLYGYTSPNGESHYSDVMRKMLPNGTGTILPATWAVGDSVVLDEQWTINVPT
                     NIPTVFMPYWPMLRAVAFVQNDATKEIMQGGRSSAAAVVPLAAITNLTSIFTCTSSID
                     PSVTLKNYGGGGDLTSADIEYGIVGQTMQTYNWSGNLGSNTSVVLTLPNLTLPGAGLQ
                     NFSAKLISVNGNAAFHNINLDYKTSVAQTTAPVTSLSQDFLATTFPPVNWILNNPDNA
                     TTWVRSAAGSLAGGSAKMNFYTYAAAGQSDYLYPYTPIDMTNAVNPQLTFSVAYCQYS
                     TENDRLQVLASTDCGVTWTSLFNEAGAALSTSPAQTTAFTPTSASQWESKTVSLANYV
                     GQPSLLLSFKATNAYGNNLYVDDINLTGVTAVHQIEGVTTLEVFPNPASDVLNIKATM
                     EKSLPMTISLTDISGRKLFQKNLGTVSVVNETVKTSDFAAGIYTLEVRSGDSVSYQKI
                     TIK"
BASE COUNT         1100 a          650 c          607 g         1157 t
ORIGIN      
        1 aactttgtgc aagcgataaa gtaggtgcac ctaaaaaaat aaccggcaat attctgccgg
       61 ttataagttt tatcatttta ttcatgaaaa gcaaatagtt atttattaat ttgattcttt
      121 agtaagtgct ttaatctctt cgtacatttt atgttcatca ccgggtgcat agcctgtttt
      181 tactgaaaca atttcgcctt ttccgttaac taagaaaaca taaggtactg ctccaatgtt
      241 gagggctctt ttcagatcac cgttggggtc aacataaact tcatagtccc aacctttgcc
      301 attgacaaaa ggtttaacgt ttgccattgt tcgtgcatca tcaacactaa ttgctacaat
      361 tttaactcct gtttctttct gccattcctc gtaatgttca ctgatggctg taagttcatt
      421 cacacaaggc ttacaccatg ttgcccaaaa atcaagcacc atcggtttgc cattgttatt
      481 taatgttgac gtgtttacag ttttaccatt cagatctttt acatcaacag caggtattgg
      541 acgtgattca aaaacctgtg ccgaagaaac tgatactgca catgaaaaga ctactactaa
      601 cttctttaaa tgattcattt atttattaaa attgaatttt atctgtttac tactaacttt
      661 tgtacagctg aactttttcc ttctacatta agcgaaataa aataaacacc agatggcaaa
      721 tcggctgttg aaagtacaat atttcctgtt gagctattta cattaaaatt cttaactgtt
      781 tttcccaaca tatttattac tttaacttca gccttgctac ctgatttaat attataatta
      841 aatgcagtta tcgaacgtgc agggttagga gatggcgcac ttatgctatt tgttttattt
      901 acttctttta cgccaaccgg tgtaatatga tagttaatgg caaactgaac tgaatcaagt
      961 ggattgtttc catcaaatac agtataggct acagtagtag taccaacata accatgtcca
     1021 agataatcac ctctgaatga tccttctgtt gcacctgcat taatagtagc aggtattgca
     1081 gatgacatca cacctactcc gtaacataaa ataccccaac aaaagttatg ctctgttccg
     1141 ggaaccttgt aaacatcata ggcattcagg ctggcatcta ttgtattctg actgttatta
     1201 attacatata cataagaatg taataaactg tcttcaacag agccataaac atctgcagat
     1261 aatcctgtat agctaaaact ttgagcttta accaaacttg ctgatgtaat taaaattagt
     1321 gctagtacaa ttttcttcat gatatttttt ttaaagctgc cccgttatct tagataccgg
     1381 gacagcaatt gatttaatta tttaattgtt attttttgat aagaaactga atcgcctgaa
     1441 cgtacttcaa gtgtataaat tcctgcagca aaatcagaag tttttacagt ttcatttact
     1501 acagaaacag tgccgagatt cttctggaac aactttcttc ctgaaatatc agttaaggaa
     1561 attgtcattg gaagtgattt ctccattgtt gctttgatat tcaatacatc acttgcagga
     1621 tttgggaaca cttcaagagt agttacacct tctatctgat gaacagctgt tacacctgtt
     1681 aaattaatat catcaacata aaggttgtta ccataagcat tagttgcttt gaaacttaaa
     1741 agtaaagatg gttgacctac ataattagct aatgaaaccg ttttagattc ccattgagat
     1801 gctgaagttg gtgtaaatgc tgtggtttgt gctggtgaag ttgataatgc tgcacctgct
     1861 tcattaaata gagatgtcca agttactcca caatcagttg atgcaagtac ctgaagacga
     1921 tcgttctccg ttgaatattg acaatatgct acactgaaag tcaactgagg attcacagca
     1981 ttagtcatat caattggtgt ataagggtaa agatagtcag attgaccagc tgcagcataa
     2041 gtataaaagt tcatttttgc agaaccacct gctaagctac cggcagcact tctaacccat
     2101 gtagttgcat tgtcagggtt atttagaatc caatttacag gaggaaatgt tgtagcaaga
     2161 aaatcctgac tgagtgatgt taccggtgct gttgtttgtg caaccgatgt tttataatca
     2221 agatttatat tatggaaagc agcattacca ttaacgctga ttaatttagc tgaaaaattc
     2281 tgtaatccag cacctggcaa agttaaatta ggtaaagtta atactacaga tgtatttgat
     2341 cccaaattgc cactccaatt ataagtttgc attgtttgtc caactatacc gtattcaatg
     2401 tccgcagaag taaggtcacc gcctcctcca taatttttta atgtgacact ggggtcaatt
     2461 gaactggtac aagtaaatat tgatgtaaga tttgtaatag ccgccaaagg cactactgct
     2521 gcagcactac ttcttccgcc ttgcataatt tctttagtgg cgtcattctg aacaaaagct
     2581 acagcacgaa gcattggcca atatggcata aacactgtag gtatattagt gggtacattt
     2641 attgtccatt gttcatccaa aacaactgaa tcaccaacag cccaagttgc cggcaatatt
     2701 gttcctgtgc cattaggcaa cattttacgc attacatcag aataatgaga ttcaccattt
     2761 ggcgaagtat aaccatataa gtccttttca gtaatggcaa tacgtgcttt taaacttgtt
     2821 aatcctgtca cagcctgtgt ggcacgaatt acggtatgag tataaataat atcgtgagta
     2881 ggtgaaaaac tatgtgtaac atcaactgta aaaggtgaag ttgttgctac acgtgagttt
     2941 aatgttgcaa caggaaatga acctgcatta ccatcccata cattgccatc taaaaatgca
     3001 tgtggtgagt agttatttgc ataaaacgat gttctgtttg ctacatcaat agtattatac
     3061 gaataataat taggcccgtt agatggaata ttattctgat acttaatcgg aatagtaaat
     3121 gcattagcat tcaatttggt gttgaaaccg ggattattag ctgcacaagg cccacaagtt
     3181 tcacctgtga attcttcaac aactacatat ctctgtgact gggcatttgc tatacccact
     3241 actaagcacg ctgccgttaa aatagtaaag atttttttca ttattataaa tatttgattt
     3301 taaaattctt ggataaaagt acacactttt cagaatacca atgtctaaga aatgccattt
     3361 ttaataaact ggtaaaatca gaccgcaagt gagtgtttaa agctatggtt attacagcgc
     3421 atcatggcaa attggtgtca cctgccattg gtctttacta caaagttgtg attcaggctt
     3481 ttggtcggtc aaaatcataa atggaatatt agtt
//
LOCUS       DAOOOI010000163         3447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4767_length_3447_cov_32.694575, whole genome shotgun sequence.
ACCESSION   DAOOOI010000163 DAOOOI010000000
VERSION     DAOOOI010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4767_length_3447_cov_32.694575"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PKX59_11230"
     CDS             complement(<1..446)
                     /locus_tag="PKX59_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ91471.1"
                     /translation="MPKSTIIITGGAGFIGSHVVRRFVNNYSDYTIYNLDNLTYAGNL
                     ANLKDIESKSNYHFIKGDITDADFVNKLFADINPDAVIHLAAESHVDRSITNPLDFIM
                     TNIVGTVNLLNAARNTWIKDKKVSPGKRFYHISTDEVYGSLGETGF"
     gene            complement(491..1516)
                     /gene="galE"
                     /locus_tag="PKX59_11235"
     CDS             complement(491..1516)
                     /gene="galE"
                     /locus_tag="PKX59_11235"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009421421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOZ91472.1"
                     /translation="MKVLVTGGTGYIGAHTVVELMNSGFDVEIIDNLNNSKEEVVDAI
                     AKITGKRPIWFNIDLCDASELKNYFSKYKPDAVVHFAAHKAVGLSVEQPLEFYRNNLL
                     SLINILQCCVDAKCNNFVFSSSCSVYADPDKLPIDENAPVKTAKSPYGNTKKIGEEII
                     TDTVKSSLLQAVSLRYFNPVGSHDSALIGELPTTMPLNVMPIITQVAIGKRKQFVVFG
                     NDYPTADGSCVRDYIHVVDVAKAHVTAVKRLLNKKNNSAYEVFNLGTGKGFSVLELIH
                     AFEKTTKQKLNYTIEGRRAGDVTAVYADTTLANTVLGWHTERDLNNMIRTAWNWELYL
                     AGRTDFI"
     gene            complement(1583..2872)
                     /locus_tag="PKX59_11240"
     CDS             complement(1583..2872)
                     /locus_tag="PKX59_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOZ91473.1"
                     /translation="MYQEILDKKAKVAVIGLGYVGLPIALEFAKKISVIGFDINAERV
                     EKMQNKIDPSGELPAEAFDGCDIKFTNSLDVLREARFFIVAVPTPIDDHKLPDLKPLV
                     GASTSIGKVLKKGDYVVYESTVYPGCTEEDCIPVLEEHSGLKFPNDFKVGYSPERINP
                     GDKEHTITKIKKVVSGCDAESLDVIAKVYETIVTAGVHRASSIKVAEAAKIIENTQRD
                     VNIALMNELSIIFNKMGINTYEVIEASSTKWNFLRFFPGLVGGHCIGVDPYYLTYKAN
                     ELGYHSQVINSGRYVNDSMGFYVAKQTVKKMIAKGKDISKSKVLVMGATFKEDVEDIR
                     NSKVTDVVNEFKSFGVTIEVTDPYASSDELMHEYGFGLVDKIANDYDAVVVAVSHKDY
                     LKMDENYFKSICKPDGVIVDIKGFLRNKIKNLQYWSL"
     gene            complement(2878..>3447)
                     /locus_tag="PKX59_11245"
     CDS             complement(2878..>3447)
                     /locus_tag="PKX59_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOZ91474.1"
                     /translation="ERYYKKGGWKGTSDMDGGTLFTQFSHFIDIMYWVFGDITDIQGK
                     FEDFTHQQNTSFEDSGFVNFKFVNGGMGSIAYTTSVWDVNLESSITVMGKYGSIKIGG
                     QYMNEVEYCHIKDYTMPKLDPVNPANDYGAYKGSAANHHYIIENVADVLRGRTSITTN
                     ALEGLKVVEIIERIYALRKIEKSEIKFHL"
BASE COUNT         1021 a          703 c          596 g         1127 t
ORIGIN      
        1 aagaaacctg tttcgccaag agagccatac acttcatcag tactgatatg ataaaatctt
       61 tttccgggag aaactttttt gtcttttatc cacgtatttc gtgcagcatt aagaaggtta
      121 accgtaccca caatattggt catgataaaa tcgagtgggt tggtaatact tctgtccaca
      181 tgcgattctg cagcaagatg aattactgca tcgggattta tatctgcaaa cagtttgttt
      241 acaaagtcag catcggtaat atcacctttt ataaaatgat agtttgattt tgattctata
      301 tctttcaggt tagcaagatt tcctgcatag gttaagttgt cgaggttgta aattgtgtaa
      361 tcggaataat tgtttacaaa tctgcgcaca acatgcgaac ctatgaatcc ggcaccgcct
      421 gtgataatga ttgtactttt tggcatgagg gtaaaattaa aaaattaaaa cttaataaag
      481 aacctttagc tcagataaag tctgttcttc cggccagata caactcccag ttccaggcag
      541 tacgtatcat attattcaaa tcgcgctctg tatgccatcc aagtacggtg ttagccaatg
      601 ttgtgtcggc ataaacagca gtgacatcac cggcacgtct tccttcaatg gtataattta
      661 atttctgttt tgtggttttt tcaaatgcat gaataagttc aagtacagaa aatccttttc
      721 ctgttcccag attaaatact tcgtaagcag agttgttttt tttgttgagc aatcgtttca
      781 cagctgtaac atgtgccttt gccacatcaa ctacatgtat ataatctctt acacaactgc
      841 catcggctgt tggataatcg tttccgaaaa ccacaaactg tttgcgttta ccaatggcta
      901 cctgagtgat gatgggcatt acattcaatg gcatggttgt aggcagttca ccgattaaag
      961 cactatcgtg tgagccaacg ggattgaaat agcgaagtga aacagcttgc agtaaagatg
     1021 attttacagt gtctgttatt atttcttctc caattttttt ggtgttgcca taaggcgatt
     1081 tagctgtttt gacaggagca ttttcatcaa taggtaattt atcaggatca gcataaacag
     1141 agcaacttga tgaaaaaaca aaattattgc actttgcatc aacacaacat tgcaaaatat
     1201 taatgagcga aagcaggttg tttcgataaa attccaatgg ttgttcaaca gaaagcccta
     1261 ctgctttatg tgctgcaaag tgtacaactg catccggttt atacttgctg aaatagtttt
     1321 ttaattcgct ggcatcacaa aggtcaatgt taaaccatat cgggcgtttg ccggtaattt
     1381 ttgcaatagc atcaacaact tcttcttttg aattattcag gttgtcaatg atttctacat
     1441 caaaaccact gttcatgagt tctacaacag tgtgtgcacc aatataaccg gtgcctccgg
     1501 tgaccaaaac tttcattaca tgcaataaat ttaccgggtt aatatttctg tataacggtt
     1561 agggcgttgc cgcactaaag gtttacagac tccagtattg aagatttttg attttatttc
     1621 ttaaaaatcc ttttatgtca acaataacac catcgggttt acagatactc ttaaaataat
     1681 tttcatccat cttaagataa tccttatgcg aaacagcaac cactacagca tcataatcat
     1741 tggcaatttt atcaaccaaa ccaaaaccat attcatgcat caactcatca gaagaagcat
     1801 aagggtcggt aacttctatt gttacaccaa atgatttaaa ttcgttcact acatccgtaa
     1861 ccttgctgtt gcggatatct tctacatctt ctttaaatgt agcacccatc accagcactt
     1921 tcgatttaga aatatccttg cctttggcaa tcattttctt tacggtttgc ttggcaacat
     1981 aaaagcccat gctgtcattt acataacgac cggagttgat gacctgtgaa tgatatccca
     2041 gttcattggc tttataggta agataataag ggtcaacacc aatacagtgt ccgcctacca
     2101 ggcccggaaa aaagcgaagg aagttccatt ttgtactgct tgcctcaatc acctcatagg
     2161 tatttattcc cattttattg aagatgatgg aaagctcatt catcagtgca atgtttacat
     2221 cacgctgtgt gttttcaata atttttgcag cttcggcaac tttaatgctg ctggcacgat
     2281 gcacacctgc tgttacaatt gtttcataaa cctttgcaat aacatcaagc gattctgcat
     2341 cgcatcctga aactactttc ttgatttttg tgatggtatg ttctttatca ccggggttaa
     2401 tacgttcagg tgagtagcct actttaaaat catttggaaa ttttaacccg gaatgttctt
     2461 caagtacggg aatacaatct tcttctgtac aacccggata aacggtactt tcatacacaa
     2521 cataatcacc ttttttcagt accttgccaa tagaagttga tgcaccaact aaaggtttca
     2581 ggtcaggtag tttatgatcg tcaataggag taggaacagc tacaataaaa aaccgcgctt
     2641 ctctaagtac gtccaatgag ttggtgaatt taatatcaca tccatcaaaa gcctcagcag
     2701 gtaattcacc gctggggtca attttatttt gcattttttc aacgcgctct gcattgatgt
     2761 cgaatcctat tacggaaatc ttttttgcaa attccagtgc aataggtaat ccaacatagc
     2821 ctaagccaat aacagcaact tttgcttttt tatctaatat ctcctgatac atttatttta
     2881 aagatgaaat tttatttctg atttttctat tttacgaagc gcataaatac gctcaatgat
     2941 ttctactact tttaatccct caagggcatt ggtagtaatt gaagtgcgac cacgcaaaac
     3001 atctgccaca ttttcaatta tgtagtgatg gttggcagca gagcctttgt aggcaccgta
     3061 atcgtttgca ggattaaccg ggtcaagttt aggcattgta tagtctttga tatgacagta
     3121 ctctacttca ttcatatact gaccgccaat ttttatagag ccgtatttcc ccattacggt
     3181 aatactgctt tcaaggttta catcccaaac agaggtagtg taggcaatac tacccattcc
     3241 accgtttaca aacttaaagt ttacaaaccc actgtcttca aatgatgtgt tttgctgatg
     3301 tgtaaaatct tcaaactttc cttgaatatc tgttatatca ccaaacaccc aatacataat
     3361 atctataaag tgcgagaact gtgtaaacaa tgttccgcca tccatgtcac ttgtaccttt
     3421 ccatccgcct tttttatagt aacgctc
//
LOCUS       DAOOOI010000164         3424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4810_length_3424_cov_15.200356, whole genome shotgun sequence.
ACCESSION   DAOOOI010000164 DAOOOI010000000
VERSION     DAOOOI010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4810_length_3424_cov_15.200356"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKX59_11250"
     CDS             complement(<1..181)
                     /locus_tag="PKX59_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91475.1"
                     /translation="MPPVIETGHAKNVANFADIISFCTGYGVQYNPGKAALKVVNLNI
                     LLTNAQQSINQVNVKN"
     gene            complement(280..1458)
                     /locus_tag="PKX59_11255"
     CDS             complement(280..1458)
                     /locus_tag="PKX59_11255"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOZ91476.1"
                     /translation="MKISALIFTVCFSVFNASAQSDLIDLLKTIESNNKSIQTNKTFW
                     EARNAEFKTGLTPYDPQVEYDYLLGSPAGAGNQRDFAVTQRLDFPTTYKRKKDLSIQQ
                     IAKTEYEQQVYRQDILLEAKLLILQAIYLNKKMTALNTRLIATTQLVKDYQKKADKGD
                     VIILDLNKARLQLLNIQNEVALTSNEILNIQTRLAELNGGIQIEIRDTNYPLTPLIPD
                     FETLDKTIETNDPVLKVYEQEIKVRQQQIAVQKSLNLPKIEVGYHAQGILGQSYKGGH
                     AGLTIPLWENRNRLNAARLNVDHSKSLAQTHILQHKAENKTYYDQLAVRAKAIEEYKS
                     LLSGLNNAYLLNKSLRLGQINIIQFFQEESYYFSAFDKYLQMELEYHQAIARLYKFEL
                     "
     gene            complement(1455..>3424)
                     /locus_tag="PKX59_11260"
     CDS             complement(1455..>3424)
                     /locus_tag="PKX59_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOZ91477.1"
                     /translation="AILVLKWFGFTINTMSLGGMAIAIGDLVDDAIIDVENVFRRLKE
                     NAAKPENERANKLKVIYDASVEIRTSVINATFIIIVAFVPLFFLSGMEGRLLTPLGIA
                     FIVALFASLIVAITITPVLCSYLLTGDKMLQKQHKESWLVRKLNAFYEQLLITAMNFK
                     RPVIITSIVLFIGAMIVFTTFGRSFLPEFNEGSLVVSVTSLPGISLEESNKIGSNVEK
                     ALLSVPEVKITTRRTGRAELDEHAQGVNASEIDAPFILKERNREAFMKDIREKLGSIS
                     GANITIGQPIGHRIDHMLSGTRANIAIKIFGTDLNTLFTLSNQVKSSIEGIDGLVDLS
                     VEQQIEIPQVQIKANRDMLAKYGITMGQFAEFTDIAFAGEKVSEVYEGNQRYDLILRF
                     DEQNRGNLEKIGNTLIDAGDGKKIPLNYVAEIVSTTGPNTINRENVQRKTVVSANVAG
                     RDQKSAVKEIKQKIERNIKLPEGYRIEYGGQFESEAEASRTLMLTSVLALFVIFFLLF
                     QEFKNVKISLIILLNLPLALIGGVLSIWLTSGILSIPAIIGFITLFGIATRNGILLVS
                     HYQTLAKEGIPLYQSIIEGSKDRLSPILMTALTAGLALIPLAIGGHLPGNEIQSPMAK
                     VILGGLLTSTLLNLFVVPVIYFLSNKKRESK"
BASE COUNT          940 a          660 c          675 g         1149 t
ORIGIN      
        1 tgttttttac gttgacctgg ttgatagatt gctgtgcatt tgtaagtagg atattgaggt
       61 taacaacttt gagtgctgct ttgcccggat tgtattggac accatatcct gtacaaaatg
      121 aaatgatgtc tgcaaagttt gctacgtttt tggcgtgtcc tgtttcgatt actggtggca
      181 taatatagtg gttttaaatt gtgagttata aaaataaaaa ggaaaattca aaaatgcaag
      241 tattgaatta aaaaaagccc tgcgcatttc tggaggggct tataactcaa acttatacaa
      301 tctggcaatc gcctgatgat attcaagttc catttgtagg tatttatcaa aggcggagaa
      361 atagtaactc tcttcttgaa agaactgtat aatattgatt tgacctaagc gcagagattt
      421 gttaagcagg taggcattgt tgagaccaga caacaaggat ttgtattctt ctatcgcctt
      481 tgctctgacg gctaactgat catagtatgt tttattttca gccttatgtt gtaaaatatg
      541 tgtctgggct aaactttttg aatgatcaac atttaaacgt gcggcattga gcctgtttct
      601 attctcccat aatggaatgg taagacctgc atgaccgcct ttatagcttt gtcctaatat
      661 tccctgtgcg tgatatccta cttcgatttt tggcaaattg agggattttt gcacagctat
      721 ttgttgttgt ctgaccttga tttcctgttc gtacaccttc agtaccggat cattggtttc
      781 aatcgtttta tctaaggttt caaaatcggg gatgagcggt gtaagcggat agtttgtatc
      841 tctaatttcg atttgtatgc cgccgttcaa ttctgcaagc cttgtttgaa tatttaatat
      901 ttcattactt gtcaatgcca cttcattttg aatgttcagt aattgtaacc tggctttatt
      961 aaggtctaaa ataattacat cacctttgtc tgcttttttc tgatagtctt ttaccaattg
     1021 ggtagttgct atcagccgtg tatttaatgc agtcattttt ttattcaagt aaatagcctg
     1081 aagtatcaac aacttagctt ccagtaaaat atcttgtctg taaacttgtt gttcatattc
     1141 ggttttagca atctgttgta tcgacagatc cttcttgcgc ttatatgttg ttgggaaatc
     1201 gagccgttga gttacagcaa agtctcgttg gttacctgcc ccggcaggag agcccagcag
     1261 ataatcatat tcaacctgcg gatcgtatgg agtgagaccc gttttaaatt ctgcatttct
     1321 tgcttcccaa aatgttttat ttgtttgtat ggacttgttg tttgattcaa ttgtttttaa
     1381 taaatcaatt aggtcgctct gagctgatgc attgaaaaca gaaaagcaaa cagtaaaaat
     1441 taatgcgctt attttcattt tgattctctt tttttattgg ataaaaaata tataacaggc
     1501 actacaaata gattgaggag tgttgaggtt aacaggcctc ctaagataac ctttgccatc
     1561 gggctttgaa tttcgttgcc cggcagatgg ccgccgatag ccagtggaat taacgcaagg
     1621 cctgcagtta aggcagtcat cagaatagga ctgagcctgt cttttgaacc ttcaataata
     1681 ctttgatata atggaatacc ttctttggct aaggtctgat agtgagacac gagcaaaata
     1741 ccatttcttg tggcgatacc aaacaaagta ataaacccga tgatggccgg aatactgaga
     1801 atgcctgatg taagccatat acttagtacg ccaccgatta atgctaaagg aagatttagt
     1861 aaaatgatca gggaaatttt tacattttta aactcttgaa acaaaaggaa aaatatcaca
     1921 aacaatgcaa gcactgaagt gagcatcaat gttcgggacg cctctgcttc tgattcaaat
     1981 tggcctccat attcgattct gtaaccttcg ggtaacttaa tattgcgttc gattttttgt
     2041 tttatttctt tcactgcact tttttgatcg cgcccggcga cgtttgccga tacaacggtt
     2101 ttgcgttgca cattttccct gttgattgta ttgggacctg tagtagagac aatttcagca
     2161 acataattga gcggtatttt tttgccgtca cctgcatcga ttaatgtatt gcctattttt
     2221 tcgagattgc cgcggttttg ttcatcaaat cgcagaatga ggtcgtagcg ttggttgcct
     2281 tcatatactt ctgatacctt ttcgcctgca aaggcaatgt cggtaaactc tgcaaactgc
     2341 cccatggtga tgccatactt tgccagcata tctctattgg ctttaatctg cacctgcgga
     2401 atttctatct gttgctcaac actcagatca accagtccat ctattccttc aatagaactt
     2461 ttaacctgat ttgataaagt aaaaagtgta tttaaatcgg tgccaaatat tttaatagca
     2521 atgttggccc gcgtaccgga aagcatgtgg tcgatacggt gacctatggg ttgaccaatg
     2581 gtaatgttag caccggaaat actgcctaat ttttctctga tatctttcat gaatgcttca
     2641 cggtttcttt ctttcagtat aaaaggggcg tctatttcgg aagcgtttac gccctgcgca
     2701 tgttcgtcga gttcagctct tccggtgcgt cttgtcgtga tttttacttc tgggacagag
     2761 agcagcgctt tttcaacatt tgacccgatc ttattacttt cttctaatga aatacccggc
     2821 aggcttgtaa cactcaccac caatgagccc tcattaaact caggcaggaa acttctgccg
     2881 aatgttgtaa atacgatcat ggcgccaata aatagtacaa tagaagtaat aataaccggc
     2941 cttttaaaat tcatagcggt aataagcaat tgctcataga atgcgtttaa ttttcgcacc
     3001 agccaacttt ctttgtgttg tttctgcaac attttgtcgc cagtgagcag gtagctgcat
     3061 agtacaggcg taatggtaat ggccactatg agagaagcaa ataatgcaac gatgaaagcg
     3121 atacccaatg gagtaagcag acgaccttcc atacccgaca gaaaaaataa aggaacgaag
     3181 gctactatta taatgaaagt tgcattgatt actgaggttc ttatttccac agacgcatca
     3241 tatattacct ttaatttatt tgctctttca ttttccggct tagcagcatt ttcttttaat
     3301 cttctgaaca cattttctac atcaataatc gcatcgtcta caaggtcgcc gattgcaatg
     3361 gccatgccgc caaggctcat tgtattgata gtaaatccaa accattttaa taccaatatc
     3421 gcag
//
LOCUS       DAOOOI010000165         3365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_4900_length_3365_cov_12.314502, whole genome shotgun sequence.
ACCESSION   DAOOOI010000165 DAOOOI010000000
VERSION     DAOOOI010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4900_length_3365_cov_12.314502"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1148
                     /gene="mqnC"
                     /locus_tag="PKX59_11265"
     CDS             3..1148
                     /gene="mqnC"
                     /locus_tag="PKX59_11265"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HOZ91478.1"
                     /translation="MNVENLLSRALDFEFLNVEEGIHLFHHAPLTDLMFVANELRKKQ
                     KPENITASGEELVTWMIDRNVNTTNVCIANCKFCNFFRVPGHKEAYITSIEEYKVKIE
                     EMFRYGGDQLLLQGGHHPELGLSFYTGIFSELKKLYPQVKLHALGPPEVAHITKLEGM
                     THTEVLKALKDSGLDTLPGAGAEILSDRVRRMISKGKCTGREWLDVMRAAHQLNITTS
                     ATMMFGHIETLQERFEHLVMLRQVQSEKPAHAKGFLAFIAWPFQDENTILKRVKGIRN
                     KVTSEEFIRMIALSRIMLPNIKNIQASWLTVGKQTAQVALHAGANDFGSIMIEENVVS
                     VAGAPHRFTSQGIQDAIREAGFIPQLRSQQYQFREIPEMMEQQVINY"
     gene            1153..>3365
                     /locus_tag="PKX59_11270"
     CDS             1153..>3365
                     /locus_tag="PKX59_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91479.1"
                     /translation="MRKISIFFLLLFGLQMEAWATHNRAGEITFNQISLLTYRVQIVT
                     YTKTSSPADRPMLEMNWGDGTQDSLPRISKTTVGIDISRNYYEGVHTFPGPAVYTVFF
                     EDPNRNGGVVNIPGSVNIPFYVESQIVINPVLGYNNSPVLLQPPIDNGAVGKIFIHNA
                     NAYDPDGDSLSYELIKCKGINGLDIPGYFYPAASTSFTLDAVTGDLIWNTPTIQGEFN
                     VAFLIREWRNGVNIGYVERDMQIDIVVTNDNPPVINALNDICVTAGTLISQNVTATDP
                     DAGNIITLSATGSPLDTNFTPISPAVFPVVTDTNSVTGTFVWQTECVHVRKAPYQVLF
                     KAQDNVNQINLVDLKRMNITVVAPAPQNLTATPVQNSIHLTWDQSVCFNAEGYRIYRR
                     IGFFGYVPSLCQTGVPAFTGYTYLATVQGLANNTFVDDNNGAGLIPGNDYCYMVIAYF
                     NDGAESYASNEACAKIVQSLPIMTNVSVTATDATNGTMYVAWSKPKEIDSIQTPGPFE
                     YRLLRGQGFLPSAFTTVSTFSNLNDTTFNDVSLNTADNAWTYKVEFHNLTQGNTFKIG
                     ESLAASSVYLTTTPTDNAIQLSWQASVPWTNSATVVYRKNGGGIYAVLDTVSGNTFND
                     TGLANDSLFCYKVETIGGYSSPGFVSPILNFSQENCNAAKDNVNPCNISSATVSGSCL
                     NSEVVLTWQKPDSACGDDVITYELYYAPSKSATPYLIVSLDGINQSTYTRNIPDST"
BASE COUNT         1009 a          625 c          731 g         1000 t
ORIGIN      
        1 gtatgaatgt agaaaattta ctgagtcgtg cactggattt tgaatttttg aatgttgaag
       61 aggggataca cctttttcat catgcaccat taaccgacct gatgtttgtt gccaatgagt
      121 tgagaaaaaa gcagaagcct gaaaatatta ctgcttctgg agaagagctt gttacctgga
      181 tgattgaccg caatgtaaat actaccaatg tatgtattgc caattgtaag ttttgtaatt
      241 ttttcagagt gccggggcat aaagaagcat acatcaccag cattgaagaa tataaggtta
      301 agattgaaga gatgtttcgt tatggtggcg atcagttgtt gctacaggga ggtcatcatc
      361 cggaattagg tttgtcattt tataccggta ttttcagtga gttaaagaaa ctttatccgc
      421 aggtaaaact acatgcactt ggcccaccgg aggtggcaca tatcactaag ttagaaggta
      481 tgacgcatac tgaagtcctt aaagccttaa aagattcagg acttgatacc ttgccgggtg
      541 caggagcaga aattttgagt gacagagtac gcaggatgat ttctaaagga aaatgtacag
      601 gtcgtgaatg gcttgatgtg atgcgggctg cacatcagtt aaacattacc acatctgcaa
      661 caatgatgtt tggtcatata gaaacattgc aggagcgttt cgaacattta gtcatgctca
      721 gacaggtgca gtcagaaaaa cctgcacatg caaaaggttt tcttgccttt attgcatggc
      781 catttcagga tgagaataca attttaaaga gagtaaaagg tattcgcaac aaagttactt
      841 ccgaagagtt tatcagaatg attgcattga gcagaattat gttgcctaac atcaaaaaca
      901 ttcaggcatc atggctcact gtaggaaagc aaacggcaca agtggccctg catgcaggag
      961 caaacgattt tggctctatc atgattgaag aaaatgtagt gagtgttgcc ggtgcaccac
     1021 atcgttttac atcacaaggt attcaggatg ctattcgcga agcaggattt attcctcaat
     1081 tgaggtcaca acaatatcag ttcagagaaa tacctgaaat gatggaacaa caggtaatca
     1141 actactgatt gtatgagaaa aatttcaatc ttttttttgc tgcttttcgg tttgcaaatg
     1201 gaagcatggg caacacacaa tcgtgcaggt gaaataactt tcaatcagat ttcattgctt
     1261 acttacagag tgcagattgt tacttatacc aaaacgagca gtcctgcaga cagacctatg
     1321 cttgaaatga attggggtga tggcacgcag gattctttgc cgcgtataag taaaactaca
     1381 gtgggcattg acatcagcag aaattattat gaaggtgttc acacttttcc cgggcctgca
     1441 gtttatactg tgttttttga agacccaaac cgcaatggtg gtgttgtaaa tattccgggc
     1501 tctgtaaata ttccatttta tgtagagtcg caaattgtca tcaatcctgt gctgggatat
     1561 aataattcac ctgtgctgtt gcagccgcca attgataatg gagccgttgg aaaaatattt
     1621 attcataatg ccaacgctta tgatcctgat ggtgatagtt tatcttatga actgataaaa
     1681 tgtaagggca tcaatggact tgatattccc ggatattttt atcctgctgc aagcacatca
     1741 tttacgttgg atgctgttac cggagattta atttggaata caccaaccat tcagggtgag
     1801 tttaatgtgg ctttcttaat tcgcgaatgg cgcaatggtg taaacattgg ctatgttgag
     1861 cgcgatatgc aaattgatat tgttgtaaca aatgacaatc ctccggtcat aaatgcattg
     1921 aatgacattt gtgttactgc cggaacatta atttctcaga atgtaacagc aaccgatcct
     1981 gatgcaggaa atattatcac cttatcggca acaggaagtc cgttagacac caactttaca
     2041 ccaataagtc ctgcagtttt tcctgttgta acggacacca attctgttac cggcactttt
     2101 gtttggcaga cagagtgtgt acatgttcgc aaagcaccct atcaggtttt gtttaaagca
     2161 caggataatg tcaatcagat aaatcttgtt gacctgaaaa gaatgaacat taccgttgtt
     2221 gcacctgcac cacagaacct tactgctaca cctgtgcaga attctattca tcttacttgg
     2281 gatcagtcgg tgtgttttaa tgcagaaggg tatcgaattt acagacgaat tggatttttt
     2341 ggatatgtgc cttcgctttg tcagacgggc gttccggctt ttacaggata tacttatctg
     2401 gcaacggttc agggattggc gaataacact tttgttgatg ataataacgg tgcagggctg
     2461 attcccggaa acgattactg ctacatggtg attgcttatt ttaatgatgg agcagagagt
     2521 tatgcctcta acgaagcctg cgctaaaatt gttcaatcac tacccattat gaccaatgtt
     2581 agtgtaactg ctactgatgc taccaatgga accatgtatg tggcatggtc taagcctaaa
     2641 gaaattgaca gcatacaaac tcccggacct tttgaatacc gattgttgcg tggacagggc
     2701 tttttaccat cagcgtttac aacagtttca acattcagca atctgaatga tactaccttt
     2761 aacgatgtat cgttgaatac tgctgacaat gcatggactt acaaagtaga atttcataac
     2821 cttacacagg gaaatacatt taagatagga gagtcgcttg ctgcttcaag tgtttatctg
     2881 actaccacac caacagacaa tgctattcaa ttgtcgtggc aggcaagcgt accttggaca
     2941 aacagtgcaa ctgttgttta cagaaaaaac ggtggtggaa tttacgcagt tttagatact
     3001 gtttcaggaa atacatttaa cgatacaggc ttagcaaacg attctttatt ctgttataag
     3061 gttgaaacaa taggaggcta ttcatcaccc ggatttgttt ctcctattct gaatttttcg
     3121 caggagaatt gtaacgcagc taaggataat gttaacccat gcaatatcag cagtgcaaca
     3181 gtatcgggtt catgcctgaa cagcgaagtt gttttaacgt ggcaaaaacc tgatagcgcc
     3241 tgtggtgatg atgtgataac ttatgaattg tattacgcgc cttcaaagag tgctacacca
     3301 tatctgattg tctctttgga tggaattaat caaagtacat acacacgtaa tattcccgac
     3361 agcac
//
LOCUS       DAOOOI010000166         3297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_5002_length_3297_cov_11.236582, whole genome shotgun sequence.
ACCESSION   DAOOOI010000166 DAOOOI010000000
VERSION     DAOOOI010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5002_length_3297_cov_11.236582"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PKX59_11275"
     CDS             <1..774
                     /locus_tag="PKX59_11275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91480.1"
                     /translation="ALVTLLSVFGIVKLNAQCSYIPATTSATDTISYTFSGGSFQSYG
                     CAPIDPTYWLSGNGKTVTISYVNPQNFPKFRVWGMNDDDSAMVTVNGNYYALTAATAS
                     YAPKVVCGISPGPDGVVFANGKVVGANNNQIGNYSYSDVQLNATNVNTIVVTGISGAG
                     WGFAGSTVYCPYVSGVSSVENQKISVYPNPVSDLLMIETENNNPNTILLYDVLGNICL
                     EKTFANKAVINVSEFLHGVYFYELRNDKKTLRFGKFLRQ"
     gene            836..1066
                     /locus_tag="PKX59_11280"
     CDS             836..1066
                     /locus_tag="PKX59_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015697087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOZ91481.1"
                     /translation="MKCPNCTTTDLVMSERQGVEIDYCPSCRGVWLDRGELDKIIDKA
                     VSNDSSQQHYQKPQHYSDHKYKKKHWLHDLFD"
     assembly_gap    1160..1208
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1227..1634
                     /locus_tag="PKX59_11285"
     CDS             1227..1634
                     /locus_tag="PKX59_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91482.1"
                     /translation="MNFKPFFLLLTLVVAQSCSLGKIDQQKGVDLTTQLLNDLKNENY
                     SDIDKYYMDSFNASEPTETKIEKFKRLREAMGPIVSFEMLLAEVKKEPVSNVNQLHIK
                     YKVVCAKVTALQTFILVNDEGQPKIIFQNVENI"
     gene            complement(1646..2632)
                     /locus_tag="PKX59_11290"
     CDS             complement(1646..2632)
                     /locus_tag="PKX59_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ91483.1"
                     /translation="MNKKFLNLIGVIAVITALASCVPARQYDEMKMRKATCDEENSKL
                     RSENTNYSTQNKELTSSVADLKREKEKLETDTTTLGTAQRRLTSLYNELNDSYEKLLR
                     NNDRMMKDKNDETKKVIGQLQMTQEELIKKQDELTAKEKKLNELTEELKKRESKVNEL
                     ESVLNKKDEDVNALRKAVSDALVGFENNGLTITKKNGKVYVSMEEKLLFASGSTVVDK
                     KGEEALKELGKVLEKNKDINVLIEGHTDNVPISGGTMKDNWDLSVLRATSVVRILIKN
                     SNIDPTRLTPAGRGPYLPVDTGNTAEARRKNRRIEIILTPKLDELFKVIGEN"
     gene            2795..3265
                     /locus_tag="PKX59_11295"
     CDS             2795..3265
                     /locus_tag="PKX59_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91484.1"
                     /translation="MATLNDNWITEAHIDFEYKKYMLLAYLQHVSESFTENKLYPPLS
                     ELMKHYRNVTALRDNKKQFYDSFPERLSSADFNNFKLMYEKIVADDSLMQEVESIILF
                     SIPQFEKHISEGKKIYDFIESKIKVSPVGIIPLQINEGYFFLSTTQNRETRVYE"
BASE COUNT         1012 a          569 c          587 g         1080 t
ORIGIN      
        1 gcattagtaa ctttattatc ggtatttggt attgtaaaac ttaatgccca gtgtagctac
       61 attcctgcaa caacatctgc aaccgacacc atttcttata ctttttccgg tggctcattt
      121 caaagttatg gctgtgcacc tattgaccct acctattggt tgtcgggaaa tggaaaaacc
      181 gtaaccatta gttatgtgaa cccgcagaat tttcctaaat tcagagtgtg ggggatgaat
      241 gatgatgact cggcaatggt gacagttaac ggcaactact atgcattaac tgcagcaact
      301 gcctcgtatg cgccaaaggt ggtgtgcggt atttctccgg gacctgacgg agttgttttt
      361 gccaatggaa aggttgttgg tgccaataat aatcagatcg gaaattattc ttattccgat
      421 gtgcagttga atgccactaa tgtgaacacc attgttgtga caggaattag cggtgcagga
      481 tggggttttg ccggttcaac agtttattgt ccttatgttt caggtgtttc atcagtagaa
      541 aatcaaaaaa ttagtgttta tccaaatcct gtttctgatt tgttgatgat tgaaacagag
      601 aataacaacc caaacacaat attgctttat gatgttttag gtaatatatg tttagaaaaa
      661 acttttgcca ataaagcggt gattaatgtc agtgaatttt tgcatggtgt gtatttttat
      721 gagttgagaa acgataaaaa aacgctcagg tttggaaaat ttttaagaca ataaacattt
      781 gaatgttttt gttgttgtaa tacattagct ttgctgataa taataacaac gaaatatgaa
      841 atgtcctaat tgtacaacaa cagacttagt aatgtcagaa cgccagggcg ttgaaattga
      901 ctactgccca tcgtgcagag gtgtttggct cgacagaggt gaacttgata aaattattga
      961 taaagctgta tcaaacgata gtagccaaca acattatcag aagccgcaac attacagcga
     1021 ccataagtac aagaagaaac attggctaca cgatttgttt gattaattag tagccagaag
     1081 tattattagt tctttaaata atttgtggtt ataatttcca agacaaatta gatcaggagt
     1141 atcaaatatt gataacagtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnngt aaagtaatat cacacaatga attttaaacc attttttctg cttttaacat
     1261 tagtagtggc gcaaagttgt tccttaggaa agattgatca gcaaaaaggt gttgacctaa
     1321 caacacaact gctgaacgat ttaaagaatg aaaactattc cgacattgat aagtactata
     1381 tggattcttt taatgccagc gagccaacag aaactaaaat agaaaaattt aaaagacttc
     1441 gtgaggcaat gggcccaata gtttctttcg aaatgttatt ggctgaagta aagaaagaac
     1501 ctgtaagtaa tgtcaaccaa ctacacatta aatacaaagt tgtctgtgca aaagtaactg
     1561 cccttcagac attcatttta gtaaatgatg aagggcagcc taaaattatt tttcagaatg
     1621 ttgaaaatat ctgaaccgct aaaaattaat tctctcctat aactttaaaa agttcgtcaa
     1681 gttttggggt taagataatt tctatgcgtc tgttttttct gcgtgcctca gcagtgttac
     1741 cggtatcaac aggcaaataa ggtcctctgc ctgcaggagt taatcttgta gggtcaatat
     1801 tggagttttt aataagaata cgaaccacac ttgtagcacg caacacactt aaatcccagt
     1861 tgtctttcat agttcctccc gaaattggca cattgtcagt atgaccttca atcaacacgt
     1921 tgatgtcttt gtttttttca agcaccttgc ccaattcctt caatgcctcc tcaccttttt
     1981 tatcaaccac ggtgcttcct gaagcaaaca acaatttctc ctccatgctc acatagactt
     2041 taccattctt tttagtgatg gtaagtccgt tattttcaaa acctacaagg gcatcactca
     2101 cagcttttct caatgcatta acatcttcat cttttttatt caatacagac tccagctcat
     2161 taacttttga ttcgcgtttt ttaagttctt ctgtcaactc attcagcttt ttctcttttg
     2221 cggtaagttc atcctgtttt tttatcagct cttcctgtgt catctgtaac tgaccgatta
     2281 cttttttagt ttcatcattc ttatctttca tcatcctgtc gttattgcga aggagctttt
     2341 catacgagtc gtttagttca ttgtaaagtg atgtcagcct gcgttgtgca gttcctaagg
     2401 tagtggtgtc tgtctctaat ttttcttttt cgcgttttaa atctgcaaca gaagaagtga
     2461 gctccttatt ttgggtagaa taattggtgt tttcactacg tagcttactg ttctcttcat
     2521 cacaggtggc ttttcgcatt ttcatctcat catattgtct tgccggaaca catgaagcca
     2581 atgcagtaat gactgcaatc acacctatta aatttaaaaa tttcttgttc attttcgttg
     2641 tgtttaaatt tattgaaact ttgtttctga atattaagta aaattataat atagtgccca
     2701 aaattaagcg tttgtcgctt tgagtttttg caagaataga tgcatcaggt ataatttttg
     2761 aacaattaaa acagacaaaa aaagtttaaa tgttatggca acactaaatg acaactggat
     2821 tacagaagca catatagact ttgagtacaa aaagtatatg ctgctagcgt atttacaaca
     2881 tgtttcagag agctttactg aaaacaaact ctatccgcca ttaagcgaac tgatgaaaca
     2941 ctatcgtaat gtaacggcat tgcgtgataa taaaaaacaa ttttatgata gttttccgga
     3001 acggctttct tctgccgatt ttaacaactt taaactcatg tacgaaaaaa ttgttgcaga
     3061 tgacagcctg atgcaggaag ttgagagtat cattctattc agtattcctc agtttgaaaa
     3121 acatatttct gagggtaaaa aaatttatga ttttatagaa tcgaaaatta aagtcagtcc
     3181 ggttggtatt attccattgc agattaatga gggttatttc tttctttcaa ccacacaaaa
     3241 ccgcgaaaca agagtgtatg aataaaaaag atgaagatgt taatgcattg agaaaag
//
LOCUS       DAOOOI010000167         3272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_5043_length_3272_cov_7.480883, whole genome shotgun sequence.
ACCESSION   DAOOOI010000167 DAOOOI010000000
VERSION     DAOOOI010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5043_length_3272_cov_7.480883"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKX59_11300"
     CDS             complement(<1..245)
                     /locus_tag="PKX59_11300"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOZ91485.1"
                     /translation="MSKVAYINGFSAYIKLEKSLSANSNEAYMHDVELLYEFLQEKHP
                     EKNAEKLTLDLLQQFIQWINERNLSARSQARIISGIKA"
     gene            373..766
                     /gene="rnpB"
                     /locus_tag="PKX59_11305"
     ncRNA           373..766
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKX59_11305"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            805..2826
                     /locus_tag="PKX59_11310"
     CDS             805..2826
                     /locus_tag="PKX59_11310"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOZ91486.1"
                     /translation="MKKKLLYISNQHEIVLKFIIFCLTAFLIIKMLPHQVRYKFDYSI
                     SKPWTEKDLIAPFDFAVLKNKDSLNAEKRILLLNATLFFERDTAVKEQVLQRAIARID
                     QIDKHEAELLKQEISILASELYKNGVADESARTVSFGQAKIIKGVDLIPANNFIFEDD
                     AVNELTRIAKEKHIDAPDKAISLLAPLVSNDLTIDKVMTDRFKDELIGTVSETRGMVK
                     KGDLIIAKGEVVTREKYQELESLKSITEEYARPNKHVLIGQVILVVLCLSLLMIFLAQ
                     RRKDIFQSNRRLSIILLMVVFDVFIYTWSLKVEAVSLYIVPLCIMPIVLRTFFDTRLA
                     LFGYIITILILGSIASNGFDFVFTQICAGMITIFSIKDMRNRSHVFWAVLFVYIGYFI
                     CYTSLEIIHAGNMVKVNWENSFWLLANVLLTLFAYPLIYFLERIFKVTSDISLIELAD
                     SNNPLLHELSIKAPGTFQHSLQVANLAESAMFKIGGNALLVRVGALYHDIGKMDMPLY
                     FIENQVTQVNPHDELSFDESAGIIISHVIKGIEKARKYNLPDLVIDFIRTHHGTSRVQ
                     YFYESYLKNYPDKIIDENAFHYPGPKPFSRETAVLMMADSVEAASRSLKKHDSESINK
                     LVEDIIDNQIAHGQFSNCNITFRDITELKKLFKKMLGSIYHVRVEYTVA"
     gene            complement(2834..>3272)
                     /locus_tag="PKX59_11315"
     CDS             complement(2834..>3272)
                     /locus_tag="PKX59_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOZ91487.1"
                     /translation="LIIIIALSSLFQPSIITLIIIFGSVLWVEPSRMIRAEVQRLRSE
                     GFVDAAIVTGFSFRQIALRHLIPNLIPVVKPVFLYGMAAVILSESGLSFIGLGVNPGT
                     VTLGGLMAQAKENIDAWWLILFPGSCIFFTILSLSALSEKNKS"
BASE COUNT         1045 a          531 c          647 g         1049 t
ORIGIN      
        1 gctttaatac ctgaaataat tcttgcctgt gaacgagcgc taagattccg ttcatttatc
       61 cactgaataa attgttgtaa gaggtctaat gtgagctttt cagcattttt ttccgggtgt
      121 ttttcctgaa gaaattcata aagcagttcc acatcatgca tataggcttc attagaattg
      181 gcagataatg atttctccaa tttaatgtat gctgaaaagc cattaatgta tgccacttta
      241 ctcattgtgc taaaatagct aaccgaaact taactcaaga gtttggtttg gtgtaattaa
      301 gtataaaaaa tcaactattg catgtttatt gattttaact aagaatcaag gataataaac
      361 cgtatttttg catcggcaag ccgctctatc gctcttaact cgtttaagag aggaaagtcc
      421 gggcaacaaa gagtatcctg cctcctgtta aaggagggtg tagttttagt gatgaagcta
      481 taacagaaag tgccacagag aaaataattc caccggcaat gtattgcatg gtggtaaagg
      541 tgaaaacgtg aggtaagagc ttacgggcat attcagtaat gaaaatgcaa ggtaaacctc
      601 aggagttgaa aggcaaaata aatcacgaac aggcaatggc tcgttgccgt ttgttcagca
      661 atgaacatct tcgtgaaggg tatgccgcaa acagacaaat gataggggat ttgaatgctg
      721 aaaagtattc agataacaga atccggctta caggcttgct ttttattttt tctgctgcat
      781 tgctttttaa taatttagca gccaatgaaa aagaagctcc tttacatcag caatcagcat
      841 gagatagttc ttaaatttat tatcttttgt cttacagcat ttttgataat aaaaatgttg
      901 ccacatcagg taaggtataa gtttgattat tctatatcta aaccctggac agagaaagat
      961 ctgattgcac cgtttgattt tgccgttctg aagaataaag attcgctgaa tgcagagaag
     1021 agaattttac ttctcaatgc aacactgttt tttgaacgtg atactgccgt taaggaacag
     1081 gttttgcaac gggcaatagc acgtattgat caaatagata aacatgaagc agagttattg
     1141 aagcaggaaa tttcaatact tgccagtgaa ctttataaga atggtgtagc tgacgaaagt
     1201 gcacgtactg tctcctttgg tcaagctaaa ataataaaag gtgttgacct gatacctgct
     1261 aacaatttta tttttgaaga tgatgccgtt aatgagctga cgcgtattgc taaagaaaaa
     1321 catattgatg ctcctgacaa ggctatttct ttacttgccc ctttggtgag taatgatttg
     1381 accattgata aagtaatgac agaccgtttt aaagatgaat tgattggtac ggtttctgaa
     1441 acgcgcggaa tggttaaaaa gggcgattta attattgcca aaggtgaagt tgtgacacgc
     1501 gaaaaatatc aggagttgga atctctgaaa tcaattactg aagaatatgc tcgtcccaat
     1561 aaacatgtac ttatcggtca agtaatattg gttgtattgt gtctttcttt attgatgata
     1621 tttcttgcgc aaagaagaaa agatattttt cagtcaaaca gaaggctaag tattatttta
     1681 ttaatggtag tttttgatgt atttatatac acatggtcgt tgaaagttga agctgtgagt
     1741 ttatatattg ttcctctctg tattatgcca attgtgttac gtacattttt tgatacacga
     1801 cttgcattgt ttggttatat catcacaatt ttaatattag gatcaattgc atcaaacggt
     1861 tttgattttg tgtttacaca aatctgtgcc ggtatgataa ctattttcag tataaaagat
     1921 atgcgcaacc gctctcatgt tttctgggca gtgttgtttg tttatatcgg ttattttatt
     1981 tgttatacca gcctcgaaat tattcatgcc ggaaatatgg ttaaagtaaa ttgggagaat
     2041 tctttctggt tgcttgctaa cgtgttgtta acactttttg cttatccatt aatatatttc
     2101 cttgagcgta tttttaaagt gacttctgat atttctttga tagaacttgc agacagtaac
     2161 aatccattgt tacatgagtt gagtataaaa gctcccggaa catttcagca ttcactgcag
     2221 gttgctaatc ttgctgaatc tgcaatgttt aaaatcggtg gcaatgcatt gttggtcaga
     2281 gtaggtgcat tgtatcatga cattggtaaa atggatatgc cgctttattt tatcgagaat
     2341 caggttacgc aagtaaatcc acacgatgaa ttgtcttttg atgaaagtgc gggtatcatc
     2401 atcagccatg tgattaaagg aatagaaaaa gccagaaagt ataatcttcc cgatctggtt
     2461 attgacttta taagaacaca tcacggaacc tcacgtgtac agtattttta tgagtcgtat
     2521 cttaaaaatt atcctgacaa gattattgat gaaaatgcat ttcattatcc cggcccaaaa
     2581 cctttcagca gagagactgc cgtattgatg atggcggatt ctgttgaagc tgcatcacga
     2641 agtcttaaga agcatgattc cgaaagtatc aataagttgg tagaagatat tatagataac
     2701 caaattgcac acggacaatt ttctaactgt aatattactt tcagagatat tactgagttg
     2761 aaaaaattat tcaagaaaat gcttggaagt atctatcatg tacgtgttga atatacggta
     2821 gcatgataaa atgtcaagat ttattttttt cgcttaaggc tgacaagctc aaaattgtaa
     2881 agaaaataca tgagcccgga aaaagtatca gccaccaggc atcaatattt tcttttgcct
     2941 gagccatcag tccacctaaa gtaacggttc cgggatttac gcctaatcca ataaatgata
     3001 atcctgactc actcaaaata acggctgcca ttccatataa gaatacaggt tttacaacag
     3061 gaattaaatt ggggattaaa tgcctcaaag caatttgtct gaatgaaaat ccggtaacaa
     3121 tagcagcatc aacaaagcct tcactacgaa gtcgctgtac ttctgccctg atcatgcgtg
     3181 atggttcaac ccaaagcacc gatccgaaaa ttatgattaa tgtgatgata gatggttgaa
     3241 acaatgatga gagtgcaatg atgataataa gt
//
LOCUS       DAOOOI010000168         3270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_5047_length_3270_cov_11.851633, whole genome shotgun sequence.
ACCESSION   DAOOOI010000168 DAOOOI010000000
VERSION     DAOOOI010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5047_length_3270_cov_11.851633"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..813)
                     /locus_tag="PKX59_11320"
     CDS             complement(31..813)
                     /locus_tag="PKX59_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HOZ91488.1"
                     /translation="MLLVADSGSTKTNWLLTDRENFTTEIETIGFNPFFHSTDFILNE
                     LKKNQKLVKYADAIKDIKFFGSGCSSDDRKNIVAAAFKIIFPNATLLVDHDMLGAALA
                     ACFDTAGLVAILGTGSNIAFWDGKHLHPTNHGLGYILGDEGSGSYFGRKLMTHFLYGI
                     MPNDLKEKFFERYHLTKESAIKNVYHLAGANVYMASFAKFLSDEASHPYIQNLIYKGL
                     TEFADTSILAYPQHQNYPVHFIGSIAFHFRNILEQIAPSGNF"
     gene            complement(836..1810)
                     /gene="pfkA"
                     /locus_tag="PKX59_11325"
     CDS             complement(836..1810)
                     /gene="pfkA"
                     /locus_tag="PKX59_11325"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOZ91489.1"
                     /translation="MNIQKIALLTSGGDSPGMNACIRSIVRSAISQKKKVVGIMRGYE
                     GMIHGEFVELNARSVSNILQRGGTILKTARSKAFMTPEGMKKAYDNLCREGIEGIIAI
                     GGDGTLKGAVEFNKVNNIPFIGIPGTIDNDLFGTDYTIGFDTALNTIIDAVDKIKDTA
                     ASHDRLFFVEVMGRDSGCLALYSGIASGAEEILMPEQKTDISALVEKLKESVQRRKTS
                     SVVIVAEGDDGGGAFKIAEAVKKSFDYDIKVTVLGHIQRGGSPTAKDRIMATRFGAEA
                     VNALLSGQTNKMTGWNGKEVTLIDLEKVVKQHNPFDKRLVSYNYFLSL"
     gene            1904..>3270
                     /locus_tag="PKX59_11330"
     CDS             1904..>3270
                     /locus_tag="PKX59_11330"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOZ91490.1"
                     /translation="MTKFTLLAAFAVLFSGASIAQDRVPVNQNPAIGARSQSVIATEF
                     TNSGQLIPRNGNSAINVAFSDDFSQQNDTSSLIARGYLPYYRGTGAQGISATWFQPDG
                     TVFPAYNGAISEYVAANYNVVTATNNIDSWLVTPALNVASGDKISFYARSVTSSTWVD
                     SIRVMYSAAGDSTPEGLTWVELGRFQASVAGTWDYINYAVTTAGATARFAIRYAVIDG
                     GPNGSNSNFIGVDQLDIYTPQTTDASVFAISSMNSGCGLSATTPVDVSIFNWGGSAIS
                     GFPVSFVVDNGTPVTETFTGTIPAGGSSSFLFTGTADFSAVGPHTIKAYSSLSGDGNA
                     ANDTTTATVTNIPPIVLGSTAVTEGFEAASGTTPPPGWTIEDTDGDGNIWDFATTYTH
                     NGLVCARLGVAQAMAGTPENWLFSPCLDLTAGTNYKIEYWYKSFTETADPKLQAMLTR
                     MNVLY"
BASE COUNT          990 a          645 c          591 g         1044 t
ORIGIN      
        1 tgggccatta cttttgacct ccgcataaat ttaaaaattt ccactcgggg caatttgctc
       61 taaaatatta cgaaaatgaa atgctatgct gccaataaaa tgtaccggat aattctgatg
      121 ttgaggatag gcgagaatac tggtgtcggc aaactcagta agacccttgt atatcagatt
      181 ttgtatgtaa ggatgcgaag cttcatcaga taaaaatttt gcaaatgatg ccatatatac
      241 atttgctccc gccagatggt aaacattttt aatagcactt tcttttgtga gatgatagcg
      301 ctcaaaaaat ttctctttta aatcatttgg cataatacca tacaaaaaat gtgtcatgag
      361 ctttctgcca aaataacttc cgctaccctc atcacctaaa atataaccca atccatggtt
      421 ggtagggtga aggtgtttac catcccaaaa agcaatgttc gatcctgttc ctaaaattgc
      481 aactaaccct gctgtgtcaa agcatgcagc taaggctgca cccaacatgt cgtggtcaac
      541 taaaagtgtt gcattgggaa aaataatttt aaatgctgct gctacaatgt tttttctgtc
      601 atcgctggaa caacccgaac caaaaaattt aatatctttt attgcatcgg catacttaac
      661 taatttttga ttttttttaa gctcgtttaa aatgaaatca gtactatgaa aaaacggatt
      721 aaatccaata gtttcaattt ctgtagtaaa attttctctg tctgttaaaa gccagtttgt
      781 ttttgtagag ccactgtctg caactaaaag cataaagttt atattagaag atgatttata
      841 aagataagaa ataattgtat gacaccaatc gcttgtcgaa aggattgtgt tgtttaacta
      901 ctttttcaag gtcaatcaaa gtaacttctt taccattcca gcctgtcatt ttattggttt
      961 gaccactgag aagtgcattt acagcttcag caccaaaccg tgtagccatt attctgtctt
     1021 tagcagttgg actgcctcca cgttgaatat gaccaagcac agtaacttta atgtcgtagt
     1081 caaaagattt ttttactgct tctgcaattt taaatgcacc gccaccatca tcaccttcag
     1141 caacaatcac cacacttgaa gtttttcttc tttgtacact ttcttttagt ttttcaacca
     1201 aagcactgat gtccgttttt tgttcaggca ttaatatttc ttcggcacca ctggcaattc
     1261 ctgaataaag tgctaagcaa ccggaatcac gacccataac ttccacaaaa aacaatctgt
     1321 catgcgatgc tgcagtgtct ttaattttat ccacagcatc aattatagta ttcaatgcgg
     1381 tatcaaaacc tattgtgtaa tctgttccaa aaagatcgtt atctattgtt cctggaatac
     1441 caataaatgg aatattattg actttattaa actcgaccgc gcctttcaat gtgccatcac
     1501 caccaatagc tataatgccc tctattcctt cacggcaaag attgtcataa gcctttttca
     1561 tgccttcagg agtcatgaaa gccttgctgc gtgctgtttt aaggatagtt ccacctcttt
     1621 gtaaaatatt gctgacagag cgggcattta attctacaaa ctcaccatga atcattcctt
     1681 cataaccccg catgatgccg actacttttt tcttttgaga aatggcgctg cgcactattg
     1741 aacgaataca ggcattcatt cccggagaat cgccaccgga agtaagtaag gcaatttttt
     1801 gaatgttcat aatttttgaa aaaagaatag gcaaatttaa tccattagaa tctctttttt
     1861 tacttttaca ccattaaacc attataaatt taaaaaatca caaatgacaa aatttacact
     1921 attagctgct ttcgcagtgt tgttttccgg tgcttccata gcgcaagacc gggttcctgt
     1981 gaatcaaaac cctgctattg gggcaagatc acagtctgta atagctacag agttcacaaa
     2041 ttcaggacaa ttgattccaa gaaatggaaa cagtgccatc aatgttgctt tttctgatga
     2101 cttcagtcaa cagaatgaca cttcttcttt gattgccaga ggttaccttc cttattacag
     2161 aggtacaggt gcgcaaggta tatctgcaac atggtttcaa cctgatggaa cagtttttcc
     2221 tgcatataat ggcgctatct ctgaatatgt agctgccaat tataatgttg taactgcaac
     2281 aaataatatt gatagctggt tggttacgcc tgcattaaat gttgcatcag gcgataagat
     2341 ttcattttat gcaagaagtg ttacaagttc tacatgggtt gattcaattc gtgtaatgta
     2401 ctctgcagcc ggtgattcta ctcctgaagg tttaacttgg gttgaattag gaagatttca
     2461 agcaagtgtt gcaggaacgt gggactatat taattatgcc gttactactg caggcgctac
     2521 tgcacgtttt gcaattcgtt atgctgttat agatggtggt cctaatggtt caaacagtaa
     2581 ttttattgga gttgaccaat tagatattta tacaccacaa acaactgatg caagtgtgtt
     2641 tgcaataagc agcatgaatt caggctgtgg tttaagtgca acaacaccag tcgatgtttc
     2701 aatattcaat tggggtggaa gcgcaatttc cggattccca gtatcatttg ttgttgataa
     2761 tggtacgcct gtaactgaaa cttttactgg aacaattccg gcaggaggtt catcatcatt
     2821 tcttttcaca ggtacagctg atttttctgc agttggacca catacaataa aagcttatag
     2881 cagtctttca ggagatggta atgcggcaaa tgatacaact actgcaactg taacaaatat
     2941 acctccaata gtactaggtt ctactgcagt aacagaaggt tttgaagctg cttcaggaac
     3001 aacaccgcct ccgggatgga caattgaaga caccgatggt gatggtaata tatgggactt
     3061 tgctactacc tatacccata atggtttagt atgtgctcgt cttggtgttg ctcaggcaat
     3121 ggcaggcact cctgaaaact ggttattctc accatgtctt gatttaactg caggaacaaa
     3181 ttataaaatt gaatattggt ataagagttt tacagaaact gctgacccaa aattgcaggc
     3241 catgttaacc agaatgaatg tattatatca
//
LOCUS       DAOOOI010000169         3267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_5054_length_3267_cov_13.328767, whole genome shotgun sequence.
ACCESSION   DAOOOI010000169 DAOOOI010000000
VERSION     DAOOOI010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5054_length_3267_cov_13.328767"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /locus_tag="PKX59_11335"
     CDS             <1..1679
                     /locus_tag="PKX59_11335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOZ91491.1"
                     /translation="TTDDISCFGISKAGNRIWMAKEVKVDKTTQDLYPDLPKATGKIY
                     DDLMYRHWTEWEDGSYSHVFIADFKNDAVGELKDIQKGERFDTPMKPNGGGEQIAWSP
                     DGNKIAYTCKKLAGKDYAVSTNSDIYLYDVASGKTENISASNEGYDMSPAFSPEGSRM
                     MWLSMQTPTYEADRNRIMIYDFATKKTMEATAGFDHTVDGAKWAPDGHRIYFISGINA
                     TDQLWVYDGRLRSYPPIRQITSDIGDYTSFSVSDNKSDPTIIATMMSISAPTEIFRID
                     VKTGASKQITNTNKNVLSTVKMGKVEKRMIKATDGKDILTWVIYPPDFDASKKYPTLL
                     YCQGGPQSTVSQFFSYRWNFQLMAANGYIIVAPNRRGLPSFGTAWNREISGDWGGQCM
                     RDYLSAIDEVSKEPFVNKDKLGAVGASFGGYSVYWLAGHHQKRFKAFISHCGVYNLES
                     MYGTTEEVWFPDFDLGGPFWQSPKPKSYTQFSPVDFVQNWDTPILVIHNEKDFRVPLS
                     QGMEAFSGARLQNIPARFLYFPDEGHWVNKPQNSVMWQRVFFDWLDRYLK"
     gene            complement(1799..>3267)
                     /locus_tag="PKX59_11340"
     CDS             complement(1799..>3267)
                     /locus_tag="PKX59_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91492.1"
                     /translation="FLVGIPPPATGWVIAYDDCCRQPLNNLGGGGQGFTIRATIYSYN
                     NLSSAPCFDSSPFFAERPYAFTCIGYPFTYNPNAIDADGDSLVYEWAYCLEQPTFTPP
                     AAFNAPSITISFNPPPYTRLNQIPGNPVLDSHSGELSFFVDTASATIGSFGSCIKVSA
                     YRCGKKIAEIFRDCAFVLTNNCYVQPGPPAINLPPVYNLPFNSGTASDTVVTAGDTVR
                     FSLHFTDIQQNMGAFPTQFITINASGSYFGTNFTIDTAGCPSPPCATLNKTLPSSSPI
                     VNSVNFQWVPNCTLINLNTCNNNPENTYYFVFDASDNFCPVPAHAYVTAKITVAGPSI
                     IQSNDTLYVNYPGAISYQWYNGGNLIPGATNSFYIVTVPGLYSCQISNTTGCNISSRL
                     MQVTQTSIAETENLFSWDIIPNPASDKVTIAINSPVANEGKIVLTDISGRIIEQQQIQ
                     LLKGRNTFNINTHSFARGMYQLCLQSGKTKLMEKLLLK"
BASE COUNT         1059 a          588 c          678 g          942 t
ORIGIN      
        1 ctacaacaga tgatatttct tgtttcggta tttcaaaagc cggtaacaga atatggatgg
       61 ctaaagaagt aaaagttgac aaaactactc aggatcttta ccccgattta ccaaaagcca
      121 caggaaaaat ttatgacgac ctgatgtatc gccactggac agaatgggaa gatggctcct
      181 actcgcatgt ttttattgct gattttaaaa atgatgctgt tggcgaactt aaagacatac
      241 aaaaaggcga acgctttgac acgcctatga aacctaatgg cggtggtgag cagatagcct
      301 ggagccctga tggaaataag attgcttaca cctgcaaaaa attggcaggt aaagattatg
      361 ccgtaagtac aaattctgat atttatcttt atgatgtagc ttcaggaaaa acagaaaata
      421 tttctgcatc aaacgaaggt tatgatatgt cgcctgcatt ttcgcctgaa ggcagcagaa
      481 tgatgtggct aagtatgcaa acaccaactt acgaagccga tcgaaacaga ataatgattt
      541 atgattttgc aacaaaaaaa actatggagg ccactgccgg atttgaccat acggttgacg
      601 gagcaaaatg ggcacctgac ggacatagaa tttattttat cagtggcatc aatgcaacag
      661 atcagctatg ggtgtatgac ggacgtttgc gcagctaccc gcctatccga caaataactt
      721 ctgacattgg tgactacact tctttttctg tttcagacaa taaaagcgac ccaactataa
      781 ttgcaaccat gatgtctatt tctgcaccta cagaaatatt tcgcattgat gtaaagacag
      841 gagcttcaaa acaaattaca aacaccaata aaaatgtgtt gagtacagtt aagatgggta
      901 aggtagaaaa acgaatgata aaagctactg acggaaaaga tattctgacc tgggtaattt
      961 atccgcccga ctttgatgca tcaaaaaaat atccgacact gctttattgt caaggtgggc
     1021 ctcaaagcac tgtatcacaa tttttcagct atcgttggaa ttttcagttg atggctgcaa
     1081 atggctatat tattgttgca ccaaacagaa gaggtctgcc atcattcggt acagcatgga
     1141 accgcgaaat ttccggtgac tggggtggac aatgtatgcg cgactacctg tctgccattg
     1201 acgaagtaag caaagagcct tttgtaaata aagataaatt aggtgccgtg ggtgcaagct
     1261 ttggtggcta tagtgtttac tggcttgccg gacatcatca gaaacgtttt aaagcattta
     1321 tctcgcattg cggagtatat aatctcgaaa gcatgtatgg aacaacagaa gaagtgtggt
     1381 ttcctgattt tgatttagga ggcccattct ggcaatcacc aaaacctaaa tcatatacac
     1441 agttttcgcc tgttgacttt gtacagaatt gggatacacc aattcttgtt attcataacg
     1501 aaaaagattt cagagtaccg ttgagtcagg gcatggaagc attttccgga gcacgactac
     1561 aaaatattcc tgcacgattt ttatatttcc ctgacgaagg acattgggtt aacaaaccac
     1621 aaaacagtgt tatgtggcag cgtgtatttt tcgattggtt ggatagatat ttgaagtaat
     1681 aaaaaagcgg gacaagcccg cttttttgtt ttgcattgga gagtttagaa atctttatag
     1741 ctatatattt tgtcttacat tattaatcaa ctccttttca gattcaatca actcaacttc
     1801 atttcaacag taatttttcc atcaactttg tttttccgga ttgcaagcac aactgataca
     1861 tacctcttgc aaaagaatgt gtgttgatgt tgaaagtatt tcggcctttt aaaagttgaa
     1921 tctgttgctg ctctattata cgaccggaaa tatcggtaag cacaatttta ccctcattag
     1981 ctaccggtga gttaatagca atagtaactt tatcagaagc cggattggga ataatgtccc
     2041 aggaaaataa attttctgtt tcagcaatag atgtttgtgt tacctgcatt aatcttgaac
     2101 tgatattaca accagttgtg ttacttattt gacatgaata caagcccgga accgtaacta
     2161 tataaaaaga attggttgct cccggaatga gattaccacc attataccat tgatatgaaa
     2221 tagcacccgg atagtttaca tacagtgtat cgtttgactg tataattgat gggcctgcaa
     2281 cagtaatttt agccgttaca taggcatgag caggtacagg acaaaagtta tctgatgcat
     2341 caaaaacaaa atagtatgta ttttccggat tgttattgca tgtattgagg ttgattaatg
     2401 tacaattcgg cacccattga aaattaacgg aatttactat tggtgatgat gatggtaaag
     2461 tcttgtttag tgttgcacat ggtggagatg gacaacctgc agtatcaata gtaaagttgg
     2521 taccaaaata tgaaccgctt gcattgatgg ttataaattg agtaggaaaa gcacccatgt
     2581 tttgttgaat atccgtaaaa tgcaacgaga atctaacagt gtcgcctgca gttacaactg
     2641 tatcagaagc agtacctgaa ttaaacggta agttatatac aggcggcaga tttattgcag
     2701 gaggccccgg ttgtacatag cagttgttag ttaagacaaa ggcacagtct ctaaaaattt
     2761 cagcaatttt ttttccgcag cgataagcag aaacttttat acaactccca aaacttccta
     2821 ttgttgcact tgcagtgtca acaaaaaaac tcaattcgcc tgaatgacta tctaatactg
     2881 ggttgccggg aatttgattt aatcgtgtgt atggtggagg attaaatgaa atggtaatac
     2941 taggagcatt aaaggctgca ggtggagtaa aggtgggttg ttctaaacag taagcccatt
     3001 cgtaaaccaa agaatctcca tctgcgtcaa tggcatttgg attataagta aatgggtaac
     3061 caatacaagt aaaagcataa ggtcgttctg caaaaaaagg tgatgaatca aagcatggcg
     3121 cagaactcaa attgttataa gaataaatgg tggcacgtat cgtaaaacct tgtccaccac
     3181 cacctaaatt atttaatggt tgtctgcagc aatcatcata agcaatcacc catcctgtag
     3241 caggaggggg tattccaaca agaaaaa
//
LOCUS       DAOOOI010000170         3254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_5091_length_3254_cov_7.218506, whole genome shotgun sequence.
ACCESSION   DAOOOI010000170 DAOOOI010000000
VERSION     DAOOOI010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5091_length_3254_cov_7.218506"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PKX59_11345"
     CDS             <1..410
                     /locus_tag="PKX59_11345"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOZ91493.1"
                     /translation="NFSEERTRFLGYCLKDKMQVIHAYRAAYIALTKNEIEQYNHQTG
                     DTEGIVNYPLSISDMMMSALFTEKDDMIKISFRSKGDFSVRELASKFFNGGGHQNAAG
                     GKFFGSLDEAVEHYIAALAYYKDQLQIPDTVKD"
     gene            451..2115
                     /locus_tag="PKX59_11350"
     CDS             451..2115
                     /locus_tag="PKX59_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOZ91494.1"
                     /translation="MNQSVQQGHPKGLYVLFVTEMWERFSYYGMRAIFILFMVKALML
                     DKAFASNLYGSYTGLVYLTPLLGGYISDRYWGNRRSIIIGGLLMAIGQFTLFFSGSMI
                     SSTPTGTDATASMLMYLGLGFLIFGNGFFKPNISTMVGQLYPKADKRVDGAFTIFYMG
                     INLGAFFAPLVCGYFGDTNDPADFKYGFLAAGIGMVIGVLTFVMLKNKYIVSPDGTPI
                     GMPKVATAATANQEGEVDIPSFNMSKILMWLAIGIGLFFVFKSLIEFDLIGSIIYAGA
                     IAVPGLIITDKSLTHVEKQRIWVIFILCFFVIFFWSAFEQAGASLTFFADEQTDRNLF
                     GWEMPASFFQSFNAIFIVIFAPIFAWLWVRLGNAGKEPGSPLKMAIGLFLLSIGYLVI
                     AFGVKGVEPGVKVSMFWLTTLYLLHTFGELCLSPIGLSMVAKLAPLRFASLLMGTWFL
                     ANSASNKFAGTLSSLYPPGQGEFKAAADKGIDLPSILNGSVTATADQLKVLAEAGIPH
                     HFPMFMGMQISSLYDFFMIFVIMSAAASVLLFIIYKWLVKMMHGVQ"
     gene            2220..2453
                     /locus_tag="PKX59_11355"
     CDS             2220..2453
                     /locus_tag="PKX59_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91495.1"
                     /translation="MKKLFTLMAVAAMFAMVSCGPSADEKAAAEKATQDSIANAEAEM
                     KAAEEAAMAASAAAATPDSAAAAAPADSAAAAH"
     gene            complement(2483..2662)
                     /locus_tag="PKX59_11360"
     CDS             complement(2483..2662)
                     /locus_tag="PKX59_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOZ91496.1"
                     /translation="MAKLTLIEVALFYINMQEIIVAIIAIAASVYLIYKFRSKSAGCD
                     KCSSGPQKSGQKKTT"
     gene            2680..>3254
                     /locus_tag="PKX59_11365"
     CDS             2680..>3254
                     /locus_tag="PKX59_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOZ91497.1"
                     /translation="MNFDRKDLSNDQLIKLYHELVRPRIIEEKMLVLLRQGKLAKWFS
                     GIGQEAVSVGAAMALHQDEYVLPMHRNLGIFSVRNIPLNRLFSQWMGKPGGFTNGRDR
                     SFHFGTQEYKIIGMISHLGPQCGIADGIALGNILKQNNKATIVFSGDGGASEGDFHEA
                     VNVAAVWDLPVIFIVENNGYGLSTPSSEQYR"
BASE COUNT          946 a          583 c          675 g         1050 t
ORIGIN      
        1 acaacttctc agaagaacga acacgttttc tcggttactg ccttaaagat aaaatgcagg
       61 taatacatgc ctacagggct gcttatattg cgctgactaa aaacgaaata gaacagtata
      121 atcatcaaac cggagataca gaaggcattg taaattatcc actaagtatc agcgatatga
      181 tgatgtcggc actttttact gagaaagacg atatgataaa aatatctttc cgctcaaaag
      241 gtgatttctc agttcgtgaa cttgcctcta aattttttaa tggcggtgga catcagaatg
      301 cagcaggagg aaagtttttt ggaagtttag acgaagcagt tgaacactat atagctgcat
      361 tagcctatta taaagaccaa ttacaaattc cggataccgt aaaagattaa tttttttaca
      421 ttagccccgc tttaaattta aatcaaacgt atgaaccaaa gtgtacaaca aggacatcct
      481 aagggactgt atgtgttatt tgtaaccgag atgtgggagc gtttcagcta ttatggaatg
      541 cgcgccatct ttattctttt catggtaaaa gcattaatgc ttgacaaagc atttgcttca
      601 aatctttatg gaagttatac cggattagtg tatctaactc cgttgttagg tggatatatt
      661 tcagatcgtt attggggaaa caggcggtca attattattg gtggcttatt gatggctatt
      721 ggtcagttta ctttgttttt tagtggatca atgattagtt ctacacctac aggtaccgat
      781 gccacagcaa gtatgctgat gtatttaggt cttggatttt tgattttcgg aaacggattt
      841 tttaaaccca atatttctac aatggtaggg cagttatatc ctaaagccga taaaagagtt
      901 gatggagcct ttacaatttt ctatatgggt atcaacctgg gtgcattctt tgcgccattg
      961 gtttgcggat actttggtga tactaacgat cctgctgact ttaaatatgg atttcttgct
     1021 gccggcatag gaatggttat tggtgtactc acgtttgtga tgctcaaaaa taaatatatc
     1081 gttagtccag atggtacacc tataggaatg ccaaaggtgg ctacagcagc tacagcaaat
     1141 caggaaggtg aagttgatat tccatcattt aacatgtcaa aaatattgat gtggttagcg
     1201 attggaatag gattgttttt tgttttcaaa tctcttattg aattcgattt aataggctca
     1261 attatttatg caggtgcaat tgcagttccc ggtcttatta ttacagataa gtctttgact
     1321 catgttgaga aacaacgcat ctgggtaatt tttatacttt gcttcttcgt aattttcttc
     1381 tggagtgcat tcgaacaggc aggtgcctca ctgactttct ttgctgacga acaaacagac
     1441 aggaatttat ttggatggga aatgcctgca agcttttttc aatcattcaa tgcaatcttt
     1501 attgtgattt ttgctccaat ctttgcatgg ctgtgggtgc gattgggtaa tgcaggaaaa
     1561 gaacccggat caccattgaa aatggcaata ggacttttct tactttcaat cggctattta
     1621 gttattgctt ttggtgtaaa aggagtcgag cccggtgtaa aagtttctat gttttggctt
     1681 acaacattat acttgctgca tacatttggt gaactttgtc tgtctccaat aggactttca
     1741 atggttgcta aattagcacc gctgcgtttt gcatctttgt taatgggaac atggttctta
     1801 gccaactctg cttcaaataa atttgccggt acactaagtt cattatatcc tcccggacaa
     1861 ggcgagttta aagctgctgc agacaaaggc attgatcttc cttctatact taatggctct
     1921 gttacagcca ctgccgatca gttaaaagtt cttgcagagg caggtattcc gcaccatttt
     1981 ccaatgttta tgggtatgca gatttcaagc ctctacgact tctttatgat atttgtaatt
     2041 atgagtgcag cagcatcagt actgctgttt atcatttaca aatggcttgt taaaatgatg
     2101 catggtgtgc aataacaatt aattgttaac aacttagttg aatagttaat aaaaaacacc
     2161 ttcatcattt attaatcagc atacttttgt cccgtcaaat caaacaacaa aaaagaaaaa
     2221 tgaaaaaatt attcacatta atggctgttg cagcaatgtt tgcaatggta tcatgcggtc
     2281 caagcgctga cgaaaaagct gctgcggaaa aagcaactca ggattcaatt gctaatgcag
     2341 aagccgaaat gaaggctgct gaagaggctg caatggctgc atctgctgcc gctgctactc
     2401 ctgattcagc tgctgccgct gcacctgcag attcagctgc tgctgctcat taattttaaa
     2461 aaattaagga aaagaaccac cgtcaggtgg tttttttttg ccctgatttt tgcggaccag
     2521 aactgcattt atcacatccg gcagatttgc tcctgaattt ataaatcaga taaacacttg
     2581 cagcaatggc tattatggcg acaattattt cttgcatatt aatataaaac aaagctactt
     2641 caattaaagt taactttgcc acactttttt tatttaacaa tgaatttcga cagaaaagat
     2701 ttgagtaacg accaactaat caaactttat catgagttgg tgcgtcctcg tataattgag
     2761 gaaaagatgc ttgtcctact tcgtcaggga aaactggcaa agtggttttc cggcatcggt
     2821 caggaagccg tttctgtagg tgctgccatg gcacttcatc aggatgaata tgttttgcca
     2881 atgcatagaa accttggcat ttttagtgta cgaaatattc ctttgaaccg tttgttttcg
     2941 caatggatgg gcaagcctgg tggattcacc aatggtcgcg atcgctcttt tcacttcggt
     3001 actcaggagt ataaaataat tggaatgata tcgcatctcg gtccgcaatg tggaattgca
     3061 gatggaattg ctctgggaaa tattcttaaa caaaataata aggcaacaat tgttttttca
     3121 ggtgatggtg gtgccagcga aggcgatttt cacgaggcag tgaatgttgc agctgtatgg
     3181 gatctgcctg ttatttttat agtagaaaat aatggttacg gattgtcaac accatcgtcc
     3241 gaacaataca gatg
//
LOCUS       DAOOOI010000171         3236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_5118_length_3236_cov_10.214084, whole genome shotgun sequence.
ACCESSION   DAOOOI010000171 DAOOOI010000000
VERSION     DAOOOI010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5118_length_3236_cov_10.214084"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /gene="asnB"
                     /locus_tag="PKX59_11370"
     CDS             <1..1687
                     /gene="asnB"
                     /locus_tag="PKX59_11370"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOZ91498.1"
                     /translation="YTIIKNGEFYNYRQHRQQLVSEGVKFASDSDTEVLLQLYIRNGI
                     KCLQYVNGFFAFCIYDSLEKTIFLARDRMGVKPLIYYSDNDKFIFASELKALMQFPIE
                     RNIDHQSLYHYFQFNYIPDPWTIYNNVNKLSPGHYMFIDLKRGALIEETCYYSIPETE
                     MLDMPDYQSVQLRLKELMTDAVGLRLVSDVPLGCFLSGGIDSSVVTALAAQQVNKLNT
                     FSIGFKDEPFFDETAFALKVAEMHNTSHHVFKLTNSELFENLEDILNYIDEPFADSSA
                     IAVFILSKYTRKHVTVALSGDGADEMFGGYQKHRAEWLIRNKPGLAPLLKIAAAVLPS
                     KFSSRESFIKNRIRQLHRFTEGISLSSKERYLRWCSISNSAYLKELILKPIDNTIENR
                     NQHLSASVKQQKSMNDVLLSDMKLVLTGDMLRKVDSMSMANSLEVRSPFLDYRVVDFA
                     FSLTDKYKIDNKAQKKIVKDAFRSLLPEEIYHRGKRGFEVPLIKWFQTELKDKIDLLL
                     GEEMIQSQALFNPNTIKQLRHAVMNNPSGESQAKMWALIVFQNWWLTHHLKSN"
     gene            1690..2880
                     /locus_tag="PKX59_11375"
     CDS             1690..2880
                     /locus_tag="PKX59_11375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ91499.1"
                     /translation="MPRVLRIINRLNLGGITYNVTYLTRFMQPEFETMLVSGVKTEDE
                     ESSEFILDQYEIKPHYIREMKREINFSSDRKAYAELKELIKKFKPDIVHTHAAKAGTL
                     GRLAAFSCNVPVVVHTFHGHVFHSYFSPLKTKLFIEIERYLAKKSSAIVTISELQKHE
                     ICNVFKVSAPEKTHIIPLGFDLSRFRENTDEKRKAFRETYKITDDVIAIGIIGRFAAI
                     KNHSFFLEAMKSVVSKTSKKVVAVLIGDGEEHESILDCCRKLNMPFAKDASESETKVV
                     LTSWIKDIDRALAGLDIVALTSHNEGTPVSLIEAQAAGKAVISTRVGGVENVVAEGHT
                     GFLCNKGDLQSFVNQLLQLVEDETLRNKMADNGWEHVRNKFHYTRLVSDMKNLYNELL
                     SAKR"
     gene            2942..>3236
                     /locus_tag="PKX59_11380"
     CDS             2942..>3236
                     /locus_tag="PKX59_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91500.1"
                     /translation="MKNIGKLLLVLVFGSLMTGCSINSSIMFKTKKDFVSQPDRTIGN
                     VEYKIAPNDILSVSVYTNEGIKLIDLTANSNSLNDATNVNTAMNGSNQYTVDID"
BASE COUNT         1100 a          505 c          634 g          997 t
ORIGIN      
        1 ctataccata attaaaaacg gtgagtttta taattatcgt cagcataggc agcaattggt
       61 gtctgaagga gtaaagtttg cttccgatag cgatacggag gtattattac agttatacat
      121 caggaatggc atcaaatgcc tgcagtatgt caatggattt tttgcttttt gtatttatga
      181 tagccttgaa aaaactattt ttttagcaag agacagaatg ggcgttaaac cattaattta
      241 ttatagtgat aatgataaat ttattttcgc ctcagaactg aaagcgttaa tgcagtttcc
      301 tatagagcga aacattgatc atcaatcgtt atatcattat tttcaattca actacatacc
      361 tgacccttgg acgatttata acaatgtaaa taaacttagt ccggggcatt atatgtttat
      421 tgaccttaag agaggggcat taattgaaga gacctgttat tattcaattc cggagactga
      481 aatgttggat atgcccgact atcaatcagt acagttgcgt cttaaagagt taatgactga
      541 tgccgtagga ttacgattag taagcgatgt gcctctagga tgttttttaa gtggtggaat
      601 agattcttcc gttgtaacag ctttagcagc acagcaggtt aataagctca atactttttc
      661 aataggattt aaggacgaac cattttttga tgaaacagca tttgcattga aggttgcaga
      721 aatgcataac acctcacatc atgtttttaa acttaccaat agcgaacttt ttgagaatct
      781 tgaggatata ttaaattata ttgacgagcc ttttgcagat tcttctgcca ttgctgtttt
      841 catactcagt aaatataccc gcaagcatgt aacagtagca ttatcaggcg atggtgcaga
      901 tgaaatgttt ggtggttacc aaaaacacag agcagaatgg ttaatcagga ataaacctgg
      961 tttggcacca ttattaaaaa tagcagcagc agttttgccc tcaaaattca gttcaagaga
     1021 aagttttatt aaaaacagaa tcaggcaatt acatcgtttt actgaaggta tttctctaag
     1081 cagtaaggag cgttatttac gctggtgctc catttcaaat tcagcttatt tgaaagaact
     1141 gattttaaaa cctattgata atacaattga aaatagaaat caacatttat cagcatcagt
     1201 taaacaacaa aaatcaatga acgatgtact gttgagcgac atgaaacttg tcctaaccgg
     1261 agatatgctt aggaaggttg acagtatgag tatggcaaat agtctggagg tgcgttcacc
     1321 atttttagat tatagggtag tggattttgc attttcgttg acagacaagt ataagattga
     1381 caataaagcc caaaagaaga tagttaaaga tgctttcaga tcactcttgc ccgaagagat
     1441 ttaccatcgt ggcaaaagag gttttgaagt gcctttaatt aaatggtttc agacagagct
     1501 aaaagataaa attgatttac tgctgggaga agaaatgatt cagagtcaag ccttatttaa
     1561 tccaaatact attaaacaat tgcgtcatgc tgttatgaat aatccatcag gtgaatctca
     1621 ggcaaagatg tgggctctca tagtttttca aaattggtgg ctcacacatc acctaaaatc
     1681 caactgatta tgccacgtgt acttcgcatt ataaacagac ttaatcttgg tggcattacc
     1741 tacaatgtta cttatctcac acgttttatg cagcctgaat ttgaaactat gttagtttca
     1801 ggagtaaaaa ccgaagacga agaaagttca gaatttatat tagatcagta tgaaataaag
     1861 cctcattaca tcagggaaat gaagcgtgaa ataaactttt catcagacag aaaagcctat
     1921 gctgaactta aagaattaat aaagaaattt aaaccggata ttgttcatac ccatgctgca
     1981 aaagcaggta cattgggccg tttggctgca ttttcatgca atgttcctgt ggttgttcat
     2041 acttttcatg ggcatgtttt tcattcttat ttcagccctc ttaaaacaaa attatttatt
     2101 gaaattgaac ggtatcttgc taaaaaaagt tcagctattg ttaccataag cgaattgcaa
     2161 aagcatgaaa tctgtaatgt ttttaaagtg agtgcacctg aaaaaacaca tatcatacct
     2221 ttaggctttg accttagtag gtttagagaa aatacagatg aaaaacgaaa agctttcagg
     2281 gagacatata aaataactga tgatgtaata gctataggca ttatcggccg gtttgctgca
     2341 ataaaaaatc attcattctt ccttgaagca atgaagtctg tagtctctaa gacctccaaa
     2401 aaagtggttg cagttttgat aggtgacggt gaggaacatg aatctatttt agattgttgt
     2461 cgtaaactga acatgccatt tgcaaaagat gcctcagagt cggaaacaaa ggttgtgctg
     2521 acatcctgga taaaggatat tgacagggct ttagccggtt tagatatagt agcgttgact
     2581 tcgcataatg aaggaactcc tgtcagtttg attgaggcac aggctgccgg gaaagcagtg
     2641 attagcacca gagttggagg tgtagaaaac gtagttgccg aaggtcatac aggattttta
     2701 tgtaacaaag gtgatttgca atcgtttgta aatcaactct tgcaacttgt ggaagatgag
     2761 acattaagaa ataaaatggc tgacaatggt tgggagcatg ttagaaataa atttcattat
     2821 acccgattgg ttagtgatat gaaaaacctt tataatgaat tactttcagc caaaagataa
     2881 aaagattact ttgctaaaaa gtttactttt gcacagcgaa aaaaaacacc atccaaataa
     2941 aatgaagaat atcggaaaat tattgttagt ccttgtgttc ggaagtctga tgacaggctg
     3001 ttcaatcaat tcaagtatca tgtttaagac aaagaaagat tttgttagtc aacctgatag
     3061 aactattgga aatgttgagt ataaaatagc acccaatgac atacttagtg ttagtgtata
     3121 tactaatgaa ggaattaagt tgattgacct tacagcaaat tcaaattcat taaacgatgc
     3181 taccaatgtt aatacagcaa tgaatggttc taatcaatat actgttgata ttgatg
//
LOCUS       DAOOOI010000172         3226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_5146_length_3226_cov_10.179123, whole genome shotgun sequence.
ACCESSION   DAOOOI010000172 DAOOOI010000000
VERSION     DAOOOI010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5146_length_3226_cov_10.179123"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="PKX59_11385"
     CDS             complement(<1..1530)
                     /locus_tag="PKX59_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOZ91501.1"
                     /translation="MEKKRTYLGNADPVAIEALYNSYLKDPQSVDQTWQTFFDGFELA
                     RKSQSPDGGNVVSAADASHIEKEFMVLRLISDYRKRGHLFTDTNPVRERRKYQPTLDI
                     QHFGLSESDLPTLFQAGQQIGIGKASLTDIITHLRDTYCRSVGVEYKYIRSPEVVKWF
                     EQKMESCKNSLEFTIDDKRKILNKLNEAVAFENFLHVKFAGQKRFSLEGAETTIPALD
                     AVIEFGAKMGIKEFVIGMAHRGRLNVLANILNKGYDEIFTEFEGKAFEDNAFGGDVKY
                     HLGYSGDVETYDGKKVHISLTPNPSHLEAVDALVQGIVRSKIDAQGGTTDDIAPILIH
                     GDAAIAAQGVVYEVIQMSLLKGFSTGGTIHLIINNQVGFTTNYIDARSSTYCTDVAKV
                     TLSPVFHVNGDDVEALIYTVKLAMEYRQKYHRDVFIDILCYRKYGHNEGDEPRFTQPL
                     LYKAIASHPNPREIYIQKLLSQGEIETNLVKEMEKSFKALLEEKLSVAKKVEKTKVPN
                     FK"
     gene            complement(1630..2349)
                     /gene="recO"
                     /locus_tag="PKX59_11390"
     CDS             complement(1630..2349)
                     /gene="recO"
                     /locus_tag="PKX59_11390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOZ91502.1"
                     /translation="MSALHNTDGIMLHLSPYGDSSVIAKIYTEKFGIQTYLVNGVRSS
                     KSKNKSVFFQPLSLLQLVVYHRPDKGLQRISNLAFANRFISIPFNITKSTQSIFMAEL
                     LYRTIREEEANPALFRFLFDNIVQLDKSLSANPDFHLLFMIKLSAFLGFRPTDNYEEH
                     LCFDMKEGCFSTKNSSHLYVIDESTSLLLHQCLNSSIKEPQLNYKQRTDLLNVLLQYY
                     QLHLPDGFRLQSMDILTELFN"
     gene            2535..2969
                     /locus_tag="PKX59_11395"
     CDS             2535..2969
                     /locus_tag="PKX59_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOZ91503.1"
                     /translation="MFSKACEHGIKAIIYIATQSMDGRRVKIGDVVENSGSPEAFTAK
                     VLGSLTKYHIVNSYTGPNGGFFIDVKDMKKIKMSDIVNAIDGDSLYNGCGMGLNECSK
                     TQPCPMHDKFIKVRKEIKEMLTTTSVYDLALGLKSGKTVLMR"
     gene            3023..>3226
                     /locus_tag="PKX59_11400"
     CDS             3023..>3226
                     /locus_tag="PKX59_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HOZ91504.1"
                     /translation="MENKEIWLPSLITKTPQEGRELAIKMARKSIAAIQTDPEVRKKL
                     RDDYANDTKQLIASANVVALEFQT"
BASE COUNT         1075 a          566 c          574 g         1011 t
ORIGIN      
        1 tttaaagtta ggaacttttg ttttctcaac ttttttggca acgcttaatt tttcttcaag
       61 taatgccttg aaagattttt ccatttcttt cactaagttt gtttctatct cgccttgtga
      121 aagtaatttc tgaatatata tttctcttgg attgggatgt gatgcaatag ctttatataa
      181 aagtggttgc gtgaaacgtg gttcatcacc ttcgttatgt ccatattttc tgtagcaaag
      241 aatatcaata aaaacatcgc gatgatattt ctgacgatat tccatggcta atttcactgt
      301 gtagatgagt gcttcaacat catcgccatt gacatgaaac accggtgata aagtaacttt
      361 tgcaacatcg gtacaatagg tgcttgaccg tgcatcaata taattggttg taaaacctac
      421 ctgattgttt atgataagat gaatagttcc tccggttgaa aagcctttga gtaacgacat
      481 ctgaatgact tcataaacta caccttgtgc agcaatggca gcatcaccat gaatgagtat
      541 gggtgcaata tcatctgttg ttccgccttg tgcatcaatt ttagaacgca caattccttg
      601 taccaatgca tctactgctt cgaggtgaga agggtttggt gttaaagaaa tatgtacttt
      661 cttgccatcg taggtttcaa catctcctga gtaaccaaga tgatatttta catcacctcc
      721 aaaggcatta tcttcaaaag ctttgccttc aaactcagta aaaatttcat cgtagccttt
      781 attcagaata ttagcaagta catttagtct gcctctatgt gccatgccaa ttacaaattc
      841 tttgatgccc attttagcac caaattcaat tactgcatca agagcaggaa ttgttgtttc
      901 ggcaccttca agagaaaaac gtttttgccc tgcaaactta acatgcagaa agttttcgaa
      961 ggcaacagct tcgttcagct tattcagaat ttttctttta tcgtcaatgg taaactcaag
     1021 actgttttta caactttcca ttttctgttc aaaccactta actacttcgg ggcttctgat
     1081 atatttatat tccacaccaa cacttcggca atacgtgtcg cgtaggtgcg taataatatc
     1141 tgtcagcgat gctttaccga taccaatttg ctgtcctgcc tgaaaaagtg taggaagatc
     1201 actctctgat aatccaaaat gttgaatatc taacgtaggt tgatattttc tgcgctcgcg
     1261 aacaggattg gtatccgtaa aaagatgtcc acgtttgcga taatcgctga ttaatcgcaa
     1321 taccataaac tctttttcta tatgtgatgc atcggctgca gaaacaacat ttccaccgtc
     1381 aggagactga ctttttcttg ccagttcaaa tccatcaaaa aaagtttgcc aggtttgatc
     1441 taccgactgt gggtctttaa gatatgagtt ataaagcgct tctattgcta ctgggtcagc
     1501 attaccgagg taagtacgtt tcttttccac tttaaaattt aactgaattc agcgcacaaa
     1561 ggtaggcaat ttgccggtgt aaaaactgat tttagttagt tgaataacca atctgcgttt
     1621 aaaaactcat caattaaaca gttcggttag tatatccatg ctttgtaaac ggaacccatc
     1681 aggaagatgt aattgataat attgtaagag aacatttagt aaatctgttc gctgtttata
     1741 atttaactgc ggttctttga ttgaagaatt aagacattga tgtaataata acgaagttga
     1801 ttcatcaata acataaagat gtgaagaatt ttttgtgctg aaacagcctt ccttcatatc
     1861 aaaacacaga tgttcctcat aattatctgt tggcctgaat ccaagaaaag cagatagttt
     1921 aatcataaat aaaagatgga aatcaggatt agccgataaa gatttatcaa gttgaacaat
     1981 gttatcaaac aaaaatctga agagagcagg attagcttcc tcttcacgaa tggtacgata
     2041 aagcaactct gccataaaaa ttgactgcgt gctttttgta atgttaaatg gaattgaaat
     2101 aaatctgttg gcaaaagcga ggttacttat gcgttgcaaa cctttatccg gacgatgata
     2161 tacaacaagt tgcagcaaac tcaacggttg aaaaaacaca ctcttgtttt ttgattttga
     2221 agaacgtaca ccattgacta aataggtttg tatgccaaac ttttccgtat aaattttagc
     2281 aataacagat gaatcgccat agggcgacag gtgcaacata attccatctg tgttatgcaa
     2341 ggcagacatt ccgcttttta gtgatttgaa tattttgtgg caaatttatg acaagatgat
     2401 gacgattcag cataaagata aaacagtttt atcacttcct gaatagaatg aaaaatagta
     2461 tatataaatt atttacgata tttttgtcgt aaattaaaaa actcactact tttgtacccc
     2521 gaaaagacgc aacaatgttt tctaaagcat gtgagcatgg cataaaagcc ataatatata
     2581 tagcaacaca atccatggat ggaaggcgtg taaaaattgg tgatgttgta gaaaactcag
     2641 gctcaccgga agcatttact gcaaaagttc tgggctcatt aactaaatat catatcgtta
     2701 attcatatac aggacctaac ggaggttttt ttattgatgt taaggatatg aaaaaaataa
     2761 aaatgagcga cattgttaat gccattgatg gcgattcttt atacaatggc tgtggtatgg
     2821 ggttgaatga gtgtagcaaa acgcaaccct gccctatgca tgataaattt attaaagtaa
     2881 gaaaagaaat aaaagaaatg ttaactacaa cttctgttta tgatttagca ttaggactga
     2941 agtctggaaa aacagtatta atgcgctaaa tattttcaaa cgaataattt tacagtacac
     3001 aaacaatcaa aataaaaata taatggaaaa taaagagata tggttaccct cgttaatcac
     3061 aaaaacacct caggaaggaa gagagcttgc aattaaaatg gcaagaaaat caattgcagc
     3121 aatacaaaca gacccagaag taagaaaaaa attgcgtgat gattatgcca acgacacaaa
     3181 acaactgata gcttctgcca atgtagtagc attagaattt caaact
//
LOCUS       DAOOOI010000173         3132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_5300_length_3132_cov_7.256419, whole genome shotgun sequence.
ACCESSION   DAOOOI010000173 DAOOOI010000000
VERSION     DAOOOI010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5300_length_3132_cov_7.256419"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>457
                     /locus_tag="PKX59_11405"
     CDS             <1..>457
                     /locus_tag="PKX59_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOZ91505.1"
                     /translation="SDTSNADSLRQAKALEKLSRIKISGYIQAQFQFADSAGQQSFNG
                     GNFPAGVDKRFTVRRGRLKVQYDGKPISKGWSITQYVLQIDATEKGLALKDAYVKLTD
                     PWVGWLSLTTGMQNRPFGYEIPFSSSTRESPERGRMSQIIFPGERDLGMM"
     assembly_gap    458..467
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <468..1270
                     /locus_tag="PKX59_11410"
     CDS             <468..1270
                     /locus_tag="PKX59_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOZ91506.1"
                     /translation="QAPKSSQWHFIKVDAGMFNGTGVRNAGLDASDFDNIKDFIGRAS
                     ISKSTHSENIKYGLGMSYYYGGYRIDTVGVYKTGVDVNGVEGFVQQYKKSDFYNVSLS
                     NRKQAYRKYYGFDAQFSIDWFPGITTIRAEYIAGDQPGFSATTVSPASVNTSDIYKRK
                     FNGAYFYFLQSIGHTPLQAVVKYDWYDPNTEVKGNNIGRNVVTGKAFNATDIRYDTWG
                     FGLAYRWDDNIKITAYYDIVKNEKSKNLSGYQKDLKDNLFTLRIQAKF"
     gene            1293..2159
                     /locus_tag="PKX59_11415"
     CDS             1293..2159
                     /locus_tag="PKX59_11415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOZ91507.1"
                     /translation="MKQLKFFAAGIFLITACNSAKQNNNSEVTNDSKKTAITIKGSDT
                     VLPLSQKEAEAYMKEHPETSITIVGGGSGVGISALLEQTTDVAMSSRPLKIDEKLKFK
                     GKNINPVETIVANDALAIVVHLSNPVDKLTHEQIADIYTGKITNWKEVGGKDAKIICY
                     SRETSSGTYEFFKEHVMDKKNYASSVLNMPATGAIIQSVSQTEGAIGYVGLAYETKDV
                     KHVAVSYDQGKNYVAPSIASAQDGSYPISRPLYYYYNQDKKDAVSSFVDFILSEKGQQ
                     LIQDVGYVPLKK"
     gene            2174..>3132
                     /gene="pstC"
                     /locus_tag="PKX59_11420"
     CDS             2174..>3132
                     /gene="pstC"
                     /locus_tag="PKX59_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOZ91508.1"
                     /translation="MKTIFKKVISKFVEGLLMMSGTVTTLAVLFIVFYLFKESLGLFN
                     MTPVEDSYSVLVNKNNPVKKLSAQQIKLVFDQEITNWKQLGGNDAEIELFRTEDISNY
                     YSEDEIGKNFEHLAQRINEHIEKHRGIIAFVASEYEMKNAASVPLSVDKITLLELMKG
                     KEWFPTAQPAAQFGVLPLLMGTLLVSLGAILLALPFGIAAAIYLAEIADTRFRNIAKP
                     LIELLAGIPSVVFGFFGLVVIVPMVQQWFNLPVGETALAGSIVLGIMALPTIITVSED
                     ALHNTPRSMKEASLALGANKWQTILRVVIPYSISGITTAVILG"
BASE COUNT         1045 a          483 c          650 g          944 t
ORIGIN      
        1 ttctgacacc tcaaatgctg acagtttacg tcaggcaaaa gcactcgaaa aattgagtcg
       61 cataaaaata agtggctata tacaggctca gtttcaattt gccgattcag caggtcagca
      121 atcttttaat ggtggaaact ttcctgccgg agtggataaa cgttttactg tgcgaagagg
      181 acgtttaaaa gtgcagtatg atggtaaacc gatttcaaaa ggttggagta ttactcagta
      241 tgttcttcaa atagatgcga cagaaaaggg tttagctctt aaagatgctt atgttaaact
      301 tacagatcct tgggtgggat ggttaagtct tacaacagga atgcaaaaca gaccatttgg
      361 ctatgaaatt cccttttcat caagtacacg tgaatcacct gaacgcggac gtatgtcgca
      421 gataatcttt cccggtgaac gcgaccttgg aatgatgnnn nnnnnnntac aggcaccaaa
      481 atcttctcag tggcatttta ttaaagttga tgcaggaatg tttaatggca caggagtgcg
      541 aaatgccggt ttggatgctt ccgattttga taatataaaa gattttattg gtcgtgcatc
      601 aatttctaaa tctacacata gtgaaaatat taagtatggt ttaggtatgt catattacta
      661 tggtggatac agaattgata cagttggtgt atataagaca ggtgttgatg tgaatggagt
      721 tgaaggcttt gttcagcagt ataagaagag cgatttttat aatgtttctc ttagcaatcg
      781 taagcaggca tatcgaaagt attatggctt tgatgctcaa tttagtattg actggtttcc
      841 tgggatcaca acaattcgtg ctgaatatat tgcaggtgat caaccgggat tttccgctac
      901 aactgtaagt ccggcttctg ttaacacttc tgatatctat aaacgaaaat ttaatggagc
      961 atacttttac ttcttacaaa gtattggaca tacaccactt caggcagttg taaaatacga
     1021 ttggtacgac ccaaatacag aggtaaaagg caataacatt ggtagaaatg ttgtgacagg
     1081 aaaagctttc aatgcaactg atatcaggta tgatacctgg ggttttggtt tagcatatcg
     1141 ttgggatgac aatatcaaga taactgcata ttatgacatt gtgaaaaatg aaaaatcaaa
     1201 aaacctaagt ggttatcaaa aagatttgaa agacaattta ttcacgctgc gtattcaagc
     1261 caagttctaa taataaacaa ttaaaataaa aaatgaaaca actgaaattt tttgcagcag
     1321 gtatttttct gattacagct tgcaacagtg ctaaacaaaa caacaattct gaagtaacaa
     1381 atgactcaaa aaaaacggca attacaatca aaggaagtga tacggttttg ccattgtcgc
     1441 agaaagaagc agaagcttat atgaaggaac atcctgaaac ttctataaca atagtaggtg
     1501 gtggttccgg tgttggcata tccgcactgt tagaacaaac aacagatgtg gctatgtcgt
     1561 cacgcccttt aaaaattgat gagaaactta aattcaaagg aaagaatata aatccagttg
     1621 aaacaatagt tgcaaatgat gcattagcta ttgtagttca tctaagtaat ccggtagata
     1681 aacttacaca tgagcaaata gccgacattt atacaggaaa gattaccaac tggaaggaag
     1741 taggaggaaa ggatgctaaa ataatttgtt attctcgtga aacgagttca ggtacttatg
     1801 agttttttaa agagcatgta atggataaaa aaaattatgc cagcagtgtt cttaacatgc
     1861 ctgcaacagg agctataata cagtctgtaa gtcaaaccga aggtgcaatt ggttatgttg
     1921 gtcttgcata cgagacaaag gatgttaagc atgtggcagt atcttatgat cagggaaaaa
     1981 attatgtagc accatcaatt gccagcgcac aagatggtag ctatccgatt tcaagaccat
     2041 tgtattatta ttataatcag gacaaaaaag atgcagtaag cagctttgtt gactttattt
     2101 tatctgagaa aggtcagcaa ttgattcagg atgttggtta tgttcctttg aaaaaataac
     2161 tacagctaag tttgtgaaaa caatatttaa aaaagttata tcaaagtttg ttgaaggctt
     2221 gctgatgatg agcggtacag taactaccct tgctgtgctt tttatcgttt tttatttgtt
     2281 caaagaatcg ttaggtttat ttaacatgac acctgttgaa gacagttatt ctgttttggt
     2341 aaataaaaat aatcctgtta agaaattatc agcgcaacaa ataaaattgg ttttcgatca
     2401 ggaaattacc aattggaagc aattaggtgg taatgatgca gaaatagaat tgtttcgtac
     2461 ggaagatatc agtaattatt attcggaaga tgaaataggt aaaaactttg aacatcttgc
     2521 ccaaagaata aatgagcaca ttgaaaaaca tcggggaata attgcatttg ttgcatcgga
     2581 atatgaaatg aaaaatgcag cttctgtgcc actgtcagta gataaaatca cactgcttga
     2641 attgatgaaa ggtaaggagt ggtttcctac agcacaaccg gcagcgcagt ttggagtact
     2701 tccgctttta atgggcacac ttttagtaag ccttggagct attcttttag cattgccttt
     2761 tggtattgca gccgcaattt atcttgcaga aattgctgac acgcgattca gaaatattgc
     2821 aaaacctttg attgaactct tagcagggat accttctgtt gtatttggtt tttttggttt
     2881 agtagttatt gtcccaatgg tgcaacaatg gtttaatctt cctgtgggcg aaacagcatt
     2941 ggcaggaagt attgttttag gaattatggc actgcctaca attattacag tatctgaaga
     3001 tgcattacac aatactcctc gtagtatgaa agaagcaagt ttggctttag gtgctaataa
     3061 gtggcaaaca attctcagag tggtaattcc ttattcaatt tcaggaataa caacagcagt
     3121 catattaggt at
//
LOCUS       DAOOOI010000174         3123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_5314_length_3123_cov_6.254237, whole genome shotgun sequence.
ACCESSION   DAOOOI010000174 DAOOOI010000000
VERSION     DAOOOI010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5314_length_3123_cov_6.254237"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PKX59_11425"
     CDS             complement(<1..361)
                     /locus_tag="PKX59_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91509.1"
                     /translation="MFRTAITSLILFFVAGNTFAQLQLFADSVIKGSSDSVRQQYQLK
                     LTDAMNKFLATEQSFNTSFDTIPSISTVTSPDGLIRFYQWVQPNMEAKTYSIYGMMQV
                     KDKKAKTIKTYTLTDGAM"
     gene            complement(435..782)
                     /locus_tag="PKX59_11430"
     CDS             complement(435..782)
                     /locus_tag="PKX59_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOZ91510.1"
                     /translation="MAIIITDECINCGACEPECPNNAIYEGGAEWRFSDGTTVKGMFN
                     KMDGTAIDADTAQAPVNLDHYYIVPDKCTECVGFHDEPQCASVCPVDCCVDDENVRES
                     KDELLAKKSRMHQ"
     gene            898..1953
                     /locus_tag="PKX59_11435"
     CDS             898..1953
                     /locus_tag="PKX59_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HOZ91511.1"
                     /translation="MSQTENIINTFIRVGQRIKEQCEQYPNGQLGDIIVSAKVNNPWF
                     TVQNQIKALEAITIMLQPDSLQQWISCYSISEHLPKNIGVVMAGNIPAVGFHDLMTVL
                     VSGNKIQIKLSSSDRILMEWITDIIKSENTELGKRISFVDKLQKPDAVIATGSDNTAR
                     YFHYYFSKVPHVIRKNRNSVAVLNGNETKEQLFALGDDIFSYFGLGCRNVSKLMVPEN
                     YNFEKFFNAIEPYGNELLLHNKYLNNYDYYRAIYLMENVPFLTNNFVHLVQQENIASP
                     VSVLHYETYSNHKVLQQSLEVNADKIQCIISDENITGKEIKFGQGQQPSASDYADGVD
                     TLRFLIEQCSDQQISNL"
     gene            complement(1950..>3123)
                     /locus_tag="PKX59_11440"
     CDS             complement(1950..>3123)
                     /locus_tag="PKX59_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOZ91512.1"
                     /translation="DYCVLWVKDTESRIDIANGFIEVYLDPMHKKGAYESVLSLKDLE
                     ATKRIAAIGKEAQWFEDHSPILPEHKKKSVTGISAKVITVINESGDAAPSTPIGINLP
                     NQEWIREKVGSKSVSLGNIVASYNYARAKSPMIDEFGENPQVKERVKKYGALAGDLHT
                     DMHEVIGHASGKINDGVAGTDVTLKNYASTLEEARADLVALYYITDKKLVDIGVMPSI
                     EVGKAEYDSYILNGLMTQLNRINPGDNLEESHMRNRQLVASWVYEKGKEANVIERITR
                     NGKTYFKINDYDKLRTLFGELLKEIQRIKSEGDYTSGKNLVENFGVKVDQDLLKEVHK
                     RYEALNIAPYMGFIQPKLVPVMQGDSIIDVKVEYNNDFLPQMLEYGKEFSFLPIKN"
BASE COUNT          945 a          584 c          550 g         1044 t
ORIGIN      
        1 ccatagcacc atctgtaagt gtatatgttt ttatggtttt ggcttttttg tcttttacct
       61 gcatcatgcc atagatgctg taggttttgg cttccatatt gggttgcacc cactgataaa
      121 atctgatgag tccatcaggt gatgttactg ttgatattga aggtatggta tcgaaggaag
      181 tgttgaatga ttgttcggta gcaagaaatt tattcatggc atcggtcagc tttaactgat
      241 attgctgcct tacggaatcg ctactgcctt taatgacact gtctgcaaac aactgtagtt
      301 gtgcaaaagt gttgcctgcg acaaaaaaca agataagaga agtaatagct gttctgaaca
      361 tgatgtataa aacgaaaaaa tctgctacat atttaaaagc gtgcagcaga ttttaaacaa
      421 atttcaatca aatattattg gtgcatgcgt gattttttcg caagtaactc atctttcgat
      481 tctctgacat tttcatcatc cacacagcag tcaaccgggc acaccgaagc acactgtggt
      541 tcgtcatgaa agccaacaca ttccgtgcat ttatctggta caatatagta atgatctaaa
      601 ttaacaggtg cttgtgctgt atcggcatca atggcagtgc catccatttt gttaaacatt
      661 ccttttacag tagtaccatc agagaaacgc cattctgctc cgccttcgta aatagcgtta
      721 ttggggcatt cgggttcgca ggctccgcag ttgatacatt cgtcagtaat tataatagcc
      781 ataattttgt gtttttattt gaagtgttgg tgcaaatata aatttatttt acaaacaagt
      841 ttttatgcta aaaagttggt ttgaacttgt tttactgaaa ttcttaaaaa aagttttatg
      901 tcacagacag aaaatatcat taataccttt atccgtgttg gacaaagaat taaggaacaa
      961 tgtgaacaat atcccaatgg acaacttgga gatattattg ttagtgccaa agtcaataac
     1021 ccttggttta cagtgcaaaa tcagattaaa gcattagagg caataactat tatgttgcaa
     1081 ccggattcgc tacaacaatg gattagctgt tacagcattt ctgaacatct gccaaaaaat
     1141 attggtgtgg tgatggcggg aaatatccct gccgtgggtt ttcacgattt gatgactgtt
     1201 ttagtttcag gcaacaaaat tcagattaaa ctctcatcat ctgacaggat tttaatggaa
     1261 tggattacag atattattaa aagtgaaaat acagagctgg gaaagcggat ttcttttgtg
     1321 gacaaactac aaaagcccga tgccgttatt gccacaggtt ctgataatac agcccgctat
     1381 ttccattatt atttcagtaa agtgccacat gttataagga aaaacagaaa tagtgtagcg
     1441 gtgttgaatg gaaatgaaac caaggaacaa ttgtttgctt taggcgatga tatcttctcc
     1501 tattttggtt taggttgccg caatgtatca aagttaatgg tgcctgagaa ttacaatttc
     1561 gaaaaattct ttaatgcaat tgaaccttat ggcaatgaac tgttattaca caacaaatat
     1621 ctgaacaact acgattatta ccgtgcaatt tatctgatgg agaatgtgcc ttttcttact
     1681 aataactttg tgcatttagt tcagcaggaa aatattgctt ctccggtttc tgtacttcat
     1741 tacgaaactt atagcaacca taaagttttg caacagtctc ttgaagtcaa tgcagataaa
     1801 attcagtgca ttatttctga cgaaaatatt accggaaaag aaataaaatt cggacaaggg
     1861 caacaaccat cggcaagtga ttatgccgat ggtgtagata cccttcgttt tttaattgaa
     1921 caatgctctg atcaacaaat cagcaatctt taatttttta ttggcagaaa ggaaaattct
     1981 tttccgtatt caagcatttg aggtagaaaa tcattgttgt attctacttt cacatctata
     2041 atgctgtcac cctgcataac aggaactaac ttaggttgaa taaagcccat gtaaggcgca
     2101 atgttcaatg cttcatatct tttgtgaact tctttcagca aatcctgatc aacttttaca
     2161 ccaaaatttt ccactaaatt ttttcctgat gtataatcac cttcagattt aattcgctgt
     2221 atttctttta gtaactcacc aaacaaggtt cttagtttat cgtaatcatt tattttgaaa
     2281 taagttttac cattacgtgt gatgcgttca ataacatttg cttctttacc tttctcataa
     2341 acccatgatg ctacaagctg acggttgcgc atgtgcgatt cttcaagatt gtctccggga
     2401 tttattctgt taagctgtgt catcaatccg tttaaaatgt atgaatcata ttctgctttt
     2461 cctacttcaa tagatggcat tacaccaata tcaactaatt tcttgtcggt gatataatat
     2521 agtgcaacca agtcggcacg agcttcttcc aatgtgcttg cataattttt taatgttaca
     2581 tctgtacctg caacaccatc atttattttt cctgaagcat gaccaattac ttcatgcata
     2641 tcggtatgaa gatcaccggc taaagctcca tattttttaa cacgttcttt cacttgagga
     2701 ttttctccaa actcgtcaat catcggactt ttggctcggg cataattata agaagctaca
     2761 atatttccta atgaaacaga cttagagcct actttttcac gaatccattc ctgattagga
     2821 agatttattc caataggagt tgatggcgca gcatcaccac tttcattaat aacggtaata
     2881 acttttgctg aaatacctgt aacacttttc tttttatgct ccggtaaaat tggtgagtgg
     2941 tcttcaaacc attgcgcttc ttttccaatt gcagcaattc gttttgtagc ttccagatct
     3001 ttaagactaa gcaccgattc gtaagcgcct ttcttatgca ttgggtcgag ataaacttca
     3061 ataaatccat tggcaatgtc aatgcggctt tcagtatctt ttacccacaa cacacaataa
     3121 tca
//
LOCUS       DAOOOI010000175         2897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_5756_length_2897_cov_8.205489, whole genome shotgun sequence.
ACCESSION   DAOOOI010000175 DAOOOI010000000
VERSION     DAOOOI010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5756_length_2897_cov_8.205489"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PKX59_11445"
     CDS             complement(<1..328)
                     /locus_tag="PKX59_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HOZ91513.1"
                     /translation="MRLPLSLLSVAIIAVACNSSKTPVQTHDELVQHIDSSVRSGDDF
                     FMFANGRWFAQNPIPSSERSNGIFRTINDTINATILQICQTAAGSTNDKGSLKQKIGD
                     YYYSGMD"
     gene            449..1879
                     /locus_tag="PKX59_11450"
     CDS             449..1879
                     /locus_tag="PKX59_11450"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91514.1"
                     /translation="MRIRYTLLSVIFILFTSNAFAWWNDILVYNGKPIAQHSMEAKSN
                     GTLYLAVPDSSAGMYSIQLLKSTNDGNTWLPVSNTTGPMATAITKTKMVHTGSDSIYC
                     LYSTIAGVYLLNVETGVIGSFTYSAVEDFDAAGSPAGNVIYLFVNEPINFTIKRYTTL
                     DGGLTWGGSTATVTTSGQTPRVYMSGTRLILNYYGPVSSDPSTSKIRAATYDESVPGT
                     IGATAFIDIATNTFVKKKQFKSVYNYNTVWFFFTEGDTSQVIKCRISTDNGDNYGPEQ
                     IVAGNNGYSNYNFDACHYANFGAWGCYLTYYNSNATLPPTPFPMMFTNASLNTPTVFS
                     TPVAYSDFNVVDTPSYHLPVITAFNGTTNDVGVAWVENSGNGSWVYFDRLSAATGMNE
                     VSQDGFISGIIFNAAELNIRFVESPKNKTTIQLSDVTGKIISQTTSVLQENLISVKMP
                     SLTSGTYLLHISDGRHSATKKLILSK"
     gene            complement(1886..2500)
                     /locus_tag="PKX59_11455"
     CDS             complement(1886..2500)
                     /locus_tag="PKX59_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91515.1"
                     /translation="MIVFVGYLASAFLAVSLLMSNAFKFRWYNLAGQIAFIIYGYLIN
                     AMPVIIANVILMIINIYQIIQLYKSKEAFDVIPIKESDPLAERFIERNKKEILEYFPK
                     FEININSGFAIMVFRDMTVANIFIADVAANGDAIVRLNYTIPKYRDYKVGKYLFELEK
                     EFLITNGLKKITYNNLNHAGHIRFINALGFSKGNDAGNYFKILN"
     gene            complement(2627..>2897)
                     /locus_tag="PKX59_11460"
     CDS             complement(2627..>2897)
                     /locus_tag="PKX59_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOZ91516.1"
                     /translation="IVNHAEKGISTTDLWIHDMYDRVKSGILARFFDNPANEGHFPRP
                     FGVFYKEERSCYEDDLLQQVEAVISTKGKGDLNKLLRGNEVWEIQ"
BASE COUNT          974 a          515 c          483 g          925 t
ORIGIN      
        1 tgtccatacc gctgtaataa taatcaccaa ttttttgttt cagacttcct ttgtcattcg
       61 tactaccggc tgccgtttga caaatttgta aaattgttgc attgatggtg tcgtttattg
      121 ttctgaaaat accattgctg cgctcgcttg atggaattgg attctgtgca aaccaacgtc
      181 cgttagcaaa cataaaaaaa tcgtcacccg agcgaacact gctgtcaatg tgttgcacca
      241 actcatcatg agtctgtacc ggtgtttttg aactattaca tgcaacagca attattgcaa
      301 ctgataacaa ggatagaggt aatcgcatgg atataaattt aggttgcgaa gataattaac
      361 tgaacaatgg acaggttaaa aaaaattcgt taaaaaaagt cttcatatac ataaagaaca
      421 taatttagta aaaaaacaaa aacagtatat gagaatacgt tatacattac tatcagttat
      481 atttattcta ttcacatcaa atgcatttgc ctggtggaac gacattttgg tttacaatgg
      541 caaacctatt gcacaacata gtatggaagc aaaaagtaat ggtacattat atcttgctgt
      601 gccagacagc agtgcaggca tgtattccat tcaattatta aaatcaacca acgatggaaa
      661 cacatggctt cctgtaagta atacaaccgg gccaatggca actgctatta caaaaactaa
      721 aatggtgcac acaggcagtg atagtattta ttgtttatat agcactattg caggtgtata
      781 tttattgaat gttgaaaccg gtgttatagg ctcatttaca tattcggctg ttgaagattt
      841 tgatgcagca ggttcaccgg caggcaatgt aatttattta tttgttaacg aaccaataaa
      901 tttcacaata aagcgctaca caacattaga tggaggttta acctggggtg gaagtacagc
      961 aactgtaaca acgtcaggac aaacgccaag agtatatatg tcaggcactc gattgatatt
     1021 aaattattat ggtccagtaa gtagcgatcc ttctacttct aaaataagag ctgccactta
     1081 tgatgaaagc gttcctggca caataggtgc tacagctttt attgatattg ccactaatac
     1141 atttgtaaaa aagaaacaat tcaaatcggt ttataactat aatacagttt ggtttttctt
     1201 tactgaaggc gacacttcac aagtaataaa atgtcgaatc agcacagata atggagacaa
     1261 ttatggacct gaacaaattg ttgcaggcaa caatggctat agcaactaca attttgatgc
     1321 ctgtcattat gcaaattttg gtgcatgggg ttgctattta acttattata attccaatgc
     1381 cacattacca cctactccat ttccaatgat gtttaccaat gcctcactta atacaccaac
     1441 tgtatttagc acaccagttg catacagtga ttttaatgtt gttgatacac cttcttatca
     1501 cttgcctgtt atcacagcct ttaacggaac taccaatgat gttggtgtgg catgggttga
     1561 aaacagtgga aacggaagtt gggtttactt tgaccgattg agtgctgcaa caggaatgaa
     1621 cgaagttagt caagatggat ttatttcagg aatcatcttt aatgctgctg aattaaatat
     1681 tcgttttgta gagtcaccaa aaaataaaac tacaattcaa ttatcagatg ttaccggaaa
     1741 aataatttct caaactactt cagtgttaca agaaaattta atttctgtaa agatgccatc
     1801 attaacatct ggaacttatt tacttcatat tagcgatggc agacattcag caacaaaaaa
     1861 acttatatta agtaagtaat cagaattagt tcaaaatttt aaaatagttt cctgcatcat
     1921 tgcctttgct gaagccaagc gcgttaataa aacgaatatg tccggcatga ttaaggttat
     1981 tataagttat cttttttaat ccatttgtga ttaaaaattc tttttctaat tcaaataaat
     2041 attttccgac cttataatct ctgtacttag ggatggtata attgagtcgt acaattgcat
     2101 caccatttgc agccacatcg gctataaaaa tattggctac tgtcatatcg cgaaaaacca
     2161 taatggcaaa gccggaattt atattgattt caaactttgg aaaatattcc aatatctctt
     2221 ttttgtttcg ttcaataaaa cgttctgcta atggatcgct ttctttaata ggaatcacat
     2281 caaatgcttc tttgcttttg taaagttgta ttatctgata aatattgatg atcatcagaa
     2341 taacattagc aataataaca ggcattgcat tgatgagata cccatatatt ataaatgcaa
     2401 tttgtcctgc caggttatac catctgaatt taaacgcatt gctcatcagc aatgaaacag
     2461 ccaaaaatgc tgatgccaga tatcctacaa atacaatcac agaatcaaat ttaagttata
     2521 aaaacaattg ttgcttaaaa accggttacg tatagccact acaactgtta tcatgaaata
     2581 aaaaaataac agtttaaaat tatggcaata aaatattatt caggatctat tgaatttccc
     2641 aaacttcatt tccacgcaaa agtttattca aatcaccttt gccttttgtt gaaataactg
     2701 cttcaacctg ttgtagtaaa tcatcttcat agcaacttct ttcttcttta tagaagactc
     2761 caaacgggcg tgggaagtgg ccttcgtttg cagggttatc aaagaacctc gctaaaattc
     2821 ctgacttaac tctatcgtac atgtcatgaa tccaaaggtc ggttgtgctg ataccttttt
     2881 ctgcatgatt aacaatg
//
LOCUS       DAOOOI010000176         2878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_5798_length_2878_cov_15.507970, whole genome shotgun sequence.
ACCESSION   DAOOOI010000176 DAOOOI010000000
VERSION     DAOOOI010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5798_length_2878_cov_15.507970"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PKX59_11465"
     CDS             complement(<1..321)
                     /locus_tag="PKX59_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91517.1"
                     /translation="MKKEKTCLECGETLVGRADKKFCDDQCRSNYNNKANSDVTAEVR
                     NINNILRKNRRILESIAGTEGKAKISKQKLSDKGFNFKYHTHQHQTQKGSVYKLCYDY
                     GYLPI"
     gene            517..855
                     /gene="ssb"
                     /locus_tag="PKX59_11470"
     CDS             517..855
                     /gene="ssb"
                     /locus_tag="PKX59_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOZ91518.1"
                     /translation="MNTLRNSVRLIGNLGAAPEVKNLDNGNKVARINLATNETYVNAK
                     GDKVTETNWHNLVAWGKTAEIAEKFLDKGKEVAVEGKLTSRSYTDKEGNKKYITEVVI
                     NEILMLDKKD"
     gene            945..1676
                     /locus_tag="PKX59_11475"
     CDS             945..1676
                     /locus_tag="PKX59_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91519.1"
                     /translation="MVRLLTFNCLLVFLAGCVNNSDEKLVQKIDSLQKSLQTIEQKLA
                     NQNSQDTIEKHEQKKTDTAITHQTVPKTIVPVVEPKNIQDTLKSKKQVQFTTPSTKLS
                     DKNVKTEESVFHYYQSTPKRVSVEITPWHDGKRELNFFDQFGNKTYSINDIRRSYSST
                     TELKEFHKNGACSLVKTSVNPDASMYWYETEIIFDENNNPQWKTELQFPQESTEQNLN
                     NKWWWNTRTNSWVKQEIIREQPVPH"
     gene            1810..2847
                     /locus_tag="PKX59_11480"
     CDS             1810..2847
                     /locus_tag="PKX59_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91520.1"
                     /translation="MINRIIYITIFLLISVNQNILSQSNSARLFPQEKINEALNKFVN
                     SAQWSKQNKQSSQSPTGFVSSPSSVLFKKVFDNNEQALMAADTIIVGAQVNDTLVITG
                     NFTHTGPILVFNSGVLIFNQATVIDTGEIYVLGNGKLFADSTTFTFPQQYIYERSLIV
                     AQNGFVQVKNSSFNYSGMSHNLYLADSAKVELTNIHQNDFTTCGLYGNANININGCNI
                     TGEYILTDQCQATFKNADTLILWHHFPDAAVVNYSFPPGDTVYNYICNNNTPGINGIG
                     YQVSADSCHNVMWALMPVNGSDVTISNSNLRLIGCWFERGDLATVSGIFNNSNYSNYT
                     APLSDTKRKCV"
BASE COUNT         1026 a          473 c          494 g          885 t
ORIGIN      
        1 aattggtaaa taaccataat cataacacaa tttatacaca cttccttttt gtgtctgatg
       61 ctgatgtgtg tgatacttaa aattgaatcc tttatcagat aatttttgct tactgatttt
      121 tgcttttcct tcagtgcctg caatagattc tagaatgcgt ctgttttttc ttaaaatatt
      181 attgatgttt cgtacttctg ctgttacatc gctattggct ttgttgttgt aattgcttcg
      241 gcattgatca tcacaaaact ttttatcagc acggccaacc aatgtttcgc cacattctaa
      301 acaggtcttt tcctttttca ttttcagagt ataatttgaa gactacaaag ataaaataat
      361 tccgattaca aacggttaca ttcggctgtt ttcggaacta actgcttatc atccgactta
      421 gaatccgggg ctgctgcact tttgcatcgt caatcggaaa caagttcaaa cgattcaaac
      481 aaaataaata atataaacaa ttaaaaacaa agtacaatga acacattaag aaacagcgta
      541 agactcatcg gaaatcttgg agcagcacca gaagttaaga atcttgacaa cggtaacaaa
      601 gtagcgcgta ttaatcttgc tacaaacgaa acgtatgtga atgccaaagg cgacaaggtt
      661 acagaaacca actggcacaa tctggtagca tggggtaaaa cagcagaaat tgcagagaag
      721 tttttggaca aaggcaaaga agttgctgta gaaggaaagt tgacttcgag aagttacaca
      781 gacaaagaag gtaacaagaa gtacattaca gaagtagtga ttaatgaaat actgatgtta
      841 gataaaaaag actaatcagt ttcatttatg gaaaaatggg agccgcagaa atgcggcttt
      901 tgttttttat ggaaaaatag aagtactttc atcttttaaa ctatatggta cgtcttttaa
      961 cttttaattg cttacttgtt tttttagccg gatgtgtaaa taattctgat gaaaagttag
     1021 ttcaaaaaat tgattcattg cagaagagtt tacaaacaat agagcaaaag ctggcaaatc
     1081 aaaatagtca ggatactatt gagaagcatg aacagaaaaa aacagatact gctataaccc
     1141 atcagacagt accaaaaacg attgtacctg tggtagagcc taagaatata caagatacat
     1201 taaaatcgaa gaagcaagta caatttacta cgccttcaac aaaactatca gataaaaacg
     1261 taaagaccga agaatctgtt ttccattatt atcaatcaac acctaaaaga gtatcagtag
     1321 aaattacacc ctggcatgat ggtaaacgcg aactgaattt tttcgaccaa tttggaaata
     1381 agacttattc gataaacgat ataagaagaa gttattcttc cacaacagaa ctcaaagagt
     1441 ttcataaaaa tggcgcatgt tctctggtaa aaacttcggt taatcctgat gcaagtatgt
     1501 attggtacga aacagaaata atttttgatg aaaacaataa cccgcaatgg aaaacagaat
     1561 tacaatttcc acaagagtca acagaacaaa acctcaataa caaatggtgg tggaatacaa
     1621 gaacaaatag ttgggtgaaa caggaaataa ttagggaaca gcctgtgccg cattaatctt
     1681 ggttttattt tagttttctt gaattcaaat gatttctgtt attattttta catttgataa
     1741 atagcaggaa caatcatgct tagttttatt acaaagctca agttgagata tgtttaaaaa
     1801 catcaaccaa tgataaatcg cataatttac attacaatat ttttgttgat ttctgtaaat
     1861 cagaatattc tttctcagag taattcagca agattatttc cgcaggaaaa aataaatgaa
     1921 gcattaaata aatttgttaa ctcagcacaa tggagcaagc agaacaagca gtcaagtcaa
     1981 tcaccaaccg gttttgtttc atcaccctca tctgtacttt tcaaaaaagt gtttgataat
     2041 aatgaacaag cccttatggc agctgatacc ataattgtag gtgcacaggt aaacgacaca
     2101 ttagtgatta caggaaactt tacacacaca ggccccattt tagtttttaa tagcggtgtt
     2161 ttaattttta atcaggcaac agttattgat acaggtgaaa tttatgttct tggtaatgga
     2221 aaattgtttg ctgactccac aacatttact tttccacaac aatatattta tgagcgaagt
     2281 cttattgtag cacaaaatgg ttttgttcaa gtaaagaatt catcatttaa ctatagcggt
     2341 atgtcacaca acttatattt agcagactcc gcaaaagtgg aacttaccaa cattcatcaa
     2401 aacgatttta caacatgcgg tttatatggt aatgctaaca taaacatcaa tggctgtaac
     2461 ataaccggtg aatatatttt aaccgatcaa tgccaggcta catttaagaa tgcagatact
     2521 ttaattcttt ggcatcattt tccggatgct gcagtagtaa attattcttt ccctcccggt
     2581 gatacggttt acaattatat atgcaacaac aacactcccg gaataaatgg aataggatat
     2641 caggttagtg cagacagttg tcataatgta atgtgggctt taatgcctgt taatggttct
     2701 gatgttacta tatcaaattc aaacttacga ttaattggat gttggtttga acgaggtgat
     2761 ttagccacag tttcgggcat ttttaataat tcaaattata gcaattatac tgcaccatta
     2821 tcggacacaa aaaggaagtg tgtataaatt gtgttatgat tatggttatt taccaatt
//
LOCUS       DAOOOI010000177         2862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_5828_length_2862_cov_5.991450, whole genome shotgun sequence.
ACCESSION   DAOOOI010000177 DAOOOI010000000
VERSION     DAOOOI010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5828_length_2862_cov_5.991450"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PKX59_11485"
     CDS             <1..236
                     /locus_tag="PKX59_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="clan AA aspartic protease"
                     /protein_id="HOZ91521.1"
                     /translation="SGLGTNDMMSAYATFDYFILGDLVLRRRKTIILDLAHINLAYET
                     MQLPLIDGVIGCDILKKYKASINFSTKKLVINI"
     gene            472..1384
                     /locus_tag="PKX59_11490"
                     /pseudo
     CDS             472..1384
                     /locus_tag="PKX59_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200547.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     assembly_gap    1385..1431
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1432..2353
                     /locus_tag="PKX59_11495"
     CDS             <1432..2353
                     /locus_tag="PKX59_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOZ91522.1"
                     /translation="QTKTKLGNNEVMGYVDQAVGDMLVHYLEMHPREAKAIVNKVILA
                     ATARHAARKARELVQRKNVLTGTGLPGKLADCSESDPGRCELFLVEGDSAGGTAKQGR
                     DRNFQAILPLRGKILNVEKAMEYKIYENEEIRNIFTALGIYRDDKQEGRPLILDKLRY
                     HKIVIMTDADVDGSHITTLILTFFFRHMKELIEQGYIYIATPPLYLVKKGKEERYCWT
                     DEERIAIVKELAGEKESSVNIQRYKGLGEMNAEQLWSTTMDPSSRTLRQVTIESAAEA
                     DHVFSMLMGDEVPPRREFIERNAKYAKVDA"
     gene            2742..>2862
                     /locus_tag="PKX59_11500"
     CDS             2742..>2862
                     /locus_tag="PKX59_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOZ91523.1"
                     /translation="MTDRIAKPFLKWAGGKTQLINDIEKALPKDISNDKFTYIE"
BASE COUNT          960 a          601 c          561 g          693 t
ORIGIN      
        1 cgtcaggact tggcaccaac gacatgatga gtgcctatgc cacattcgat tattttatat
       61 tgggcgattt ggtcttgcgc agacgaaaaa ctatcattct cgaccttgca cacattaacc
      121 ttgcctacga aacaatgcag ttgccattga ttgatggggt gataggttgc gacatcctta
      181 aaaaatataa agcaagcatc aatttttcta ctaaaaaact tgtaattaac atctgatttt
      241 tttacattaa aagccttaaa aacagcgtta ataacttgct cgactttgtt aaaaaaaacg
      301 tgcatttcca caccgttttt atgtgaaagc agcctttatt ttttgtaaat ttgaacaatt
      361 ataaaaaacg acagtttacg aaaagttatg agtgatcaaa cagtagaaaa tttagctgaa
      421 atggaagtaa aaaatactta ttcggccgac agcattcagg tattggaggg gttggaggca
      481 gtaaggaaac gccctgccat gtacattggc gatgttggtg taaaaggatt gcaccacttg
      541 gtttatgagg tagttgacaa ttcaattgac gaagcccttg ccggacattg caaaaacatc
      601 agtgtcacca ttggagaaga caactcaata actgttaaag atgatggtag aggtattcct
      661 accgaaatgc acagcaaaga aaaaaagtct gcactcgaag tagccatgac agtgctgcat
      721 gccggtggca aattcgacaa ggactcttat aaagtttccg gtggattaca tggtgttggt
      781 gtatcgtgcg taaatgcatt gagcacacac atgcgcacag aagtacaccg caacggaaaa
      841 atttatgtgc aggaatacag cattggtaaa ccattgtatt ctgttaaaga gataggtact
      901 actgattaca gaggcaccat tcaaaccttc aagcctgatg acacaatttt tatcacaaca
      961 atttacaact acgacacatt agcttcacgc ctgcgcgaac tatcttacct taacaaaggc
     1021 atccgcatca cattaaccga catgcgcgaa aaagacgagc aaggcaatgt tcgctctgac
     1081 gagtttttaa gtgagttggg cttgcgcgaa tttgttgaat acattgatgc caatcgcgaa
     1141 aagctcatcc ctgaatgtat gttttttgag agtgataaat tcggaattcc aattgaagtg
     1201 gccatgcaat acaacacttc ttttgcagag aatctccact cctatgtaaa caacatcaac
     1261 acacatgaag gtggcacaca cctttccggt ttcagacgtg cactcacacg tacattaaaa
     1321 gcctatgccg aaaaagaagg catgcttgcc aaattaaaat tcgatattga tggtgacgat
     1381 ttcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacaaacaaa
     1441 aacaaagctt ggtaacaacg aagttatggg ttatgttgat caggccgttg gcgacatgct
     1501 tgtccactac ctcgaaatgc atccgcgcga agcaaaggct attgtcaaca aagtaatact
     1561 tgctgctacg gcacgccatg ctgcacgcaa agcacgcgag ctggtgcaac gcaaaaacgt
     1621 actcactgga acaggactac ccggcaaatt agccgactgc tcagagagcg accccggacg
     1681 atgtgaactc ttccttgtcg agggagactc tgccggtgga actgccaaac aaggccgcga
     1741 cagaaatttc caagccatac ttccactacg cggaaaaatc ctcaacgtag aaaaagcaat
     1801 ggaatacaaa atctacgaaa acgaagagat aagaaacatc ttcactgccc ttggcatcta
     1861 tcgtgacgac aaacaagaag gtcgcccgct catactcgac aaactgcgct atcacaaaat
     1921 cgtaatcatg accgatgccg atgttgacgg tagccacatc accacactca tcctcacatt
     1981 cttcttccgc cacatgaaag aactcataga gcagggctac atctacattg caacaccacc
     2041 gctctacctc gtaaaaaaag gaaaagaaga gcgctactgc tggacagacg aagagcgcat
     2101 tgccatcgtc aaagaactgg ctggcgaaaa agaaagcagc gtaaacatac agcgctacaa
     2161 aggtcttggc gaaatgaatg ccgaacaact ttggtcaacc accatggacc cctcaagccg
     2221 caccttgcga caggtcacca tagaaagtgc tgccgaagcc gaccatgttt tcagcatgct
     2281 catgggcgat gaagtgccac cgcgcagaga gtttatagaa cgcaatgcta aatatgcaaa
     2341 agtagatgca taacaaactc taaaaatcac aaaaagctgt cccacaaagg cagctttttt
     2401 tgtgggctaa tactaaattg agactcacat tttataattc aaataaatca ttggaaagat
     2461 caaggcagtc taatttataa caacattcaa acttttaaac tcaattacaa tacaaggtag
     2521 gattttgtat tatttttatt taactatgat ttattgaaat atattgctgt attacttttt
     2581 acattgataa agtttagaac ttcgtacttt cgtgagttat ttacaatcct taaaggcgac
     2641 cgagcagaca aaatcggttg acaattaacc gaccgagaca aagaataaga agaatgaaaa
     2701 ttgtaaattt gcttgaagca aaaaactaat agaaaagact aatgacagac agaatagcaa
     2761 aaccgttttt aaagtgggct ggtggcaaaa cacagctcat taacgacatt gaaaaggctt
     2821 tgccaaaaga catttctaat gacaaattca catacattga ac
//
LOCUS       DAOOOI010000178         2823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_5900_length_2823_cov_8.943642, whole genome shotgun sequence.
ACCESSION   DAOOOI010000178 DAOOOI010000000
VERSION     DAOOOI010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5900_length_2823_cov_8.943642"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1112)
                     /locus_tag="PKX59_11505"
     CDS             complement(114..1112)
                     /locus_tag="PKX59_11505"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOZ91524.1"
                     /translation="MKKLLGLFLLTVLTTFQTFAQGDFLKDSLDNYINREMQRWQIPG
                     VAIAIVKDGKVITTKGYGLRETGKPDKVDEFTLFQIASNTKAFTGTALALLDYQKRIS
                     LDDKVTKYLPWFQLKDPCATEMVTIKDILCHRIGFYTFQSDFLNWDCNMTTDALIKNM
                     RNVQPVNEFRAKYGYCNMGYVTAGEVIKAVTDTSWQDFFDYHFFKPLQMNRSSVFHQK
                     IVTDANAAKPYTLVDNKLVLLDYANIDNIGACASINSCVNDISHWIMMQLNKGKYENK
                     EVIPSAVINTTQNSYMIVRETGSARMPSNHFQNYGLGWKICMVTKLYRMMAVPMAL"
     gene            1315..1974
                     /locus_tag="PKX59_11510"
     CDS             1315..1974
                     /locus_tag="PKX59_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOZ91525.1"
                     /translation="MFNTEKYKKICEELLPYKANLIAVTKTKPVTDIEAAIQNGQMLF
                     GENYVQELVEKVPVLPKNIQWHFIGNLQRNKVKQIAPFIHTVQTVDSRKLLNELNKHA
                     ASNNRVIKVLLQVHIAKEEAKFGFSEVEVQMLVDEGVFEQYNNISFNGLMGMATNTDD
                     TMQVRSEFKQLYGLYKSLSDAFDRNESPVLSMGMTSDYKIALDEGSNMIRIGSALFGS
                     R"
     gene            1979..2596
                     /locus_tag="PKX59_11515"
     CDS             1979..2596
                     /locus_tag="PKX59_11515"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ91526.1"
                     /translation="MGLFFALITTLSWSIGIFPFTEASRKLGVNSLNHFRLFLAVALL
                     FATALLINHSEFINLFNEQYVQAWIWFGLSGVVGLALGDYFYFGLYAIMGARIGSIFS
                     TLSPAAALVTSYFFVDERINFIGVIGIFLTMAGVIFISISKSENLSAEEGRYGSDVKG
                     VLFGVLAAFCQGVGLVLAKKGFVVLEQEGKFIHPINATFVRMVVS"
     gene            2609..>2823
                     /locus_tag="PKX59_11520"
     CDS             2609..>2823
                     /locus_tag="PKX59_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOZ91527.1"
                     /translation="MITTIFRKWKEVLRPLKSNSAGLKYAAVGTFFGPVFGVCMSLYT
                     IAVLEATVAQTIFSLVPVVAALIARYF"
BASE COUNT          869 a          457 c          539 g          958 t
ORIGIN      
        1 atttgcaaaa taattaatca gttgctgacg taatgctccg taaaaccaat tagcatcagt
       61 attagtcaga atcacaaaac ctgcattcag ttcaggtaat aaagtggtgt tggtcacaaa
      121 gccattggca ccgccatcat gcgatacaat tttgtaacca tacatatctt ccatccaagg
      181 ccgtaatttt gaaagtgatt tgaaggcata cgtgcagagc ccgtctctct tacaatcata
      241 taagaatttt gagtagtgtt aatcactgca gaaggaatca cttctttgtt ttcatattta
      301 cctttgttta attgcatcat tatccaatgt gagatatcgt taacgcatga gtttattgat
      361 gcgcaggcac caatgttgtc aatgtttgca tagtccaata aaactaattt gttgtcaact
      421 agtgtatatg gttttgccgc attcgcatct gttacaattt tttgatgaaa tacactgctg
      481 cgattcattt gtaacggttt aaaaaaatga taatcgaaaa aatcctgcca tgaagtatca
      541 gtaactgctt tgataacctc gcctgctgtt acatagccca tattacaata tccatacttg
      601 gcacgaaact catttacggg ctgaacattt ctcatatttt ttattaatgc atcagttgtc
      661 atattgcaat cccaattcaa aaaatcactt tgaaaggtat aaaaaccaat tctatggcag
      721 aggatatctt taattgtaac catttctgtt gcacatggat ctttaagttg aaaccagggg
      781 agatatttag ttactttatc atctaatgaa atccgctttt gataatctaa gagtgccaat
      841 gcagttcctg taaaagcctt cgtattgctg gcaatttgaa ataaagtaaa ctcatctacc
      901 ttgtccggtt tccctgtttc tctcaatcca tagcctttcg tggtgattac ctttccatct
      961 tttacaatgg caatggcaac acctggtatt tgccatcgtt gcatttcacg attaatgtag
     1021 ttgtcaagac tgtcttttaa aaaatcgcct tgtgcaaatg tttgaaaggt ggttaacact
     1081 gttaacagaa aaagacctaa gagttttttc atttcgataa atttaatgta tcaaatgtat
     1141 ggaattttaa atatacaagc acctgcattg tagtttatcc ggaataccgg ttttactttc
     1201 tgagagcaag ctatgccaat gggtgtggct tgcttatttt tgtagaaaga tttttctaaa
     1261 ctataaatga gtctcagata aattacgaat tttaccgttg attatctgac tcagatgttt
     1321 aatacagaga aatacaaaaa aatatgtgaa gagttgttgc cttataaagc caaccttatt
     1381 gcagttacca aaactaaacc tgttacagat attgaggctg ccattcaaaa tggacaaatg
     1441 ctttttggcg agaattatgt gcaggaactg gtggagaagg tgcctgtctt accgaaaaat
     1501 attcaatggc attttatcgg caatctacaa cgcaataaag taaaacagat tgcaccattt
     1561 attcatactg tacaaacggt tgatagtaga aaacttttaa atgaattaaa taaacatgct
     1621 gcatcaaata acagagtaat taaagtgctt ttacaggtac atattgcaaa agaagaagca
     1681 aagtttgggt tctctgaagt tgaagtgcaa atgttggttg atgaaggggt gtttgaacaa
     1741 tacaacaata ttagttttaa tggattgatg gggatggcaa ctaataccga tgatactatg
     1801 caagtgcgtt ctgagttcaa gcagttgtat ggattgtata aatcactttc cgatgcattt
     1861 gacagaaatg agtctccggt attaagtatg ggcatgacat ccgattataa aattgcatta
     1921 gatgaaggaa gtaatatgat tcgaataggt tcagcattgt ttggaagcag atagttatat
     1981 gggattgttc tttgcactta ttacaacact ttcctggtct atcggtattt ttccatttac
     2041 agaagccagc cgaaaacttg gagtaaattc acttaatcat ttcagattat ttcttgctgt
     2101 agctttactt ttcgcaacag cattattaat aaatcattca gagtttatca atcttttcaa
     2161 tgagcagtat gttcaggctt ggatttggtt tggactttca ggtgttgttg ggttagcatt
     2221 aggtgactat ttttattttg gactctatgc aataatggga gccaggatag gaagtatttt
     2281 ttctacactt tcaccggcag cagcattagt aacatcttat ttttttgttg acgaacgtat
     2341 taattttatt ggtgtaatag gaatatttct caccatggca ggagtaatat ttatttctat
     2401 ttcaaaaagt gaaaacctgt cagcagaaga aggcaggtat ggaagtgacg ttaaaggtgt
     2461 tttgtttggt gttcttgcag ctttttgtca gggagttgga ttggtacttg ccaagaaagg
     2521 ttttgttgta ttagaacagg aagggaaatt tattcatcca attaatgcaa catttgtcag
     2581 aatggttgtt tcgtaggcaa ctataatttt gataactaca atatttagaa aatggaagga
     2641 ggttttgcga ccactaaaat caaatagtgc agggctaaaa tatgcagcag taggaacttt
     2701 tttcggacct gtttttggtg tctgtatgtc gctttataca attgctgtac ttgaagccac
     2761 agttgcacaa acaatttttt cattagtacc tgttgtggca gcattaatag cccgttattt
     2821 cta
//
LOCUS       DAOOOI010000179         2798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_5964_length_2798_cov_30.961356, whole genome shotgun sequence.
ACCESSION   DAOOOI010000179 DAOOOI010000000
VERSION     DAOOOI010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5964_length_2798_cov_30.961356"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PKX59_11525"
     CDS             <1..293
                     /locus_tag="PKX59_11525"
                     /inference="COORDINATES: protein motif:HMM:NF019982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ91528.1"
                     /translation="KQTLDLMMKADNEVLMQTLEAMAEREESCSNLSSIKFPVLILCG
                     EHDVLTPPEKSEFMQQQISGSLLHIIENSGHLGHLENKDAFNQHLNNFLKSF"
     gene            complement(290..1549)
                     /locus_tag="PKX59_11530"
     CDS             complement(290..1549)
                     /locus_tag="PKX59_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOZ91529.1"
                     /translation="MRIKTILITLLAIVNLRANAAQILIPMDDTQTNHLKAYGIAYWV
                     LQSGVEINWMLNYRGGSFMFIQNPKFESELKLRGVSYNVISEGQANQIVSEILDPQVN
                     QDVVKLEKVPKICVYSPKDKRPWDDAVTLALTYAEIPYDVVYDEEIVQGKLALYDWMH
                     LHHEDFTGQYGKFYNMYRNATWYQEQQRSMESMAKQLGFAKVSQMKLAVAKKIKEFVA
                     GGGFLFTMCSGTDTYDIALSAEGLDICESMYDGDGTSPNYNDHLDFSKTFAFRNYTLS
                     VNPLEYRFSNIDVTTTRRVPRDEDVFTLFDFSAKWDPVPTMLCQDHMQVIKGFMGQTT
                     AFNKNYIKPDVIIMGENKAANEAKYIHGDFGKGTWTFYGGHDPEDYQHMVGDPPTDLN
                     LHPNSPGYRLILNNVLFPAAKKKKQKT"
     gene            complement(1565..>2798)
                     /locus_tag="PKX59_11535"
     CDS             complement(1565..>2798)
                     /locus_tag="PKX59_11535"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOZ91530.1"
                     /translation="YLIAIAVTNYAAYSDYVPMAGGDSLQVLNYVYPENLSSAQTQTP
                     DIIPIIQLYDSLTIPYPFSHEKYGHAQFNWGGGMEHQTMSFMVNFSQDLMAHECAHQW
                     FGDLVTCGSWEDIWLNEGFATYFEGLTKERYYPSLWYNWKKQKINNITSQPDGSVFCT
                     DTNDVSRIFNGRLTYNKGSYLLHMLRWQLGDSVFFNALTSYLNDTLLKYSFAKTPDLI
                     AHLQNSSGQNLSTFFNQWYYNQGYPSYHITWNQNNPSVWLKIDQTTSHPSVSFFEMSV
                     PVRFYGNGTDTTIVFNHTFSGQQFTASIPWQVDSVVFDPDLWILSANNLVTTGIETKD
                     LSNENLQIYPNPVRNFINVLCKTNCKYIITDASGKTISKGELHQKNNRIILNSKPGIY
                     FLNTSDENRWRSQSFIIQ"
BASE COUNT          882 a          531 c          486 g          899 t
ORIGIN      
        1 taaaacaaac attagacctt atgatgaagg ctgataatga agtgttgatg caaacgttgg
       61 aagcaatggc agaaagagaa gagtcatgta gcaatctttc ttcaattaaa tttcctgtat
      121 taattctttg tggcgaacat gatgtgctta cacctcctga aaaatcagaa ttcatgcagc
      181 agcaaatttc cggcagtctt ttacatataa ttgaaaattc cggccactta ggccatcttg
      241 aaaataaaga tgcatttaat caacacctga ataacttcct taaatcgttt taagtttttt
      301 gctttttctt cttagcagca ggaaataaaa cattgttcag aatcagtcgg tagcccggac
      361 tgttcggatg taaattcaag tctgtaggtg ggtcgccaac catgtgctga tagtcttccg
      421 ggtcgtgacc tccgtaaaat gtccaagtgc ccttgccaaa atctccatgt atgtatttag
      481 cttcattagc agctttattt tcacccataa tgataacatc aggctttata taatttttgt
      541 tgaatgcagt tgtctgaccc ataaaacctt taatcacctg catgtggtcc tgacaaagca
      601 tagtaggaac agggtcccac ttagctgaaa aatcaaataa agtaaacaca tcttcatcgc
      661 gcggtactct tcttgttgtt gtgacatcaa tattcgaaaa acgatattca agtggattaa
      721 cacttaaagt ataattccga aatgcaaatg tttttgaaaa atcaagatgg tcgttataat
      781 ttggagaagt gccgtcacca tcatacatgc tttcgcaaat atctaaacct tctgctgaaa
      841 gcgcaatgtc atacgtgtcg gtaccggaac acatagtaaa caagaatcca ccacctgcaa
      901 caaactcttt tattttcttt gcaacggcaa gtttcatttg tgacacttta gcaaatccaa
      961 gttgtttggc catgctttcc atacttcttt gctgctcctg ataccatgtt gcatttctat
     1021 acatattata gaattttcca tactgtccgg taaaatcttc gtgatgtaag tgcatccagt
     1081 catacaatgc taatttgccc tgaacaatct cttcatcata aacaacatca taaggaattt
     1141 cagcataggt taaagcaagc gtcactgcat catcccatgg gcgtttgtct ttaggagaat
     1201 aaacacaaat tttaggaact ttctctaatt taacaacatc ctgattgact tgtggatcta
     1261 atatttcaga gacaatctga ttggcctgac cttcagaaat gacattgtaa ctaacacctc
     1321 gcagttttaa ctcactttca aatttcggat tctgaataaa cataaaactt ccacctctgt
     1381 aattcaacat ccagttaatt tctactccgc tttgtaaaac ccaataagcg ataccataag
     1441 cttttaggtg attggtttgt gtatcatcca ttggaataag aatctgtgca gcattagcac
     1501 gcagatttac tattgcaaga agtgtgatta atattgtttt aatccgcatg acctttaatg
     1561 tttgctattg aataatgaat gattgactcc gccatctgtt ttcgtctgat gtatttaaaa
     1621 aataaatacc cggttttgaa ttcaatatta tccggttgtt tttttgatgt aattctcctt
     1681 ttgaaattgt tttgccactg gcatcagtaa tgatgtattt acaatttgtt ttgcataata
     1741 cattgatgaa attacgcaca gggttagggt aaatttgtag attttcgtta ctcaaatctt
     1801 ttgtttcaat tcctgttgta acaagattgt ttgctgataa aatccataaa tcaggatcaa
     1861 acacaacaga atctacctgc catgggattg acgcagtaaa ttgttgtccc gaaaaagtat
     1921 gattaaatac aatagtagta tctgtaccat ttccataaaa cctgacaggt acagacattt
     1981 caaaaaatga gactgaagga tgtgatgtag tctgatcaat ttttagccaa acagatgggt
     2041 tattctgatt ccatgtaata tgataagacg ggtaaccctg attgtaatac cattgattaa
     2101 agaaagtact cagattctgc ccacttgagt tttgcagatg tgctattaag tcaggtgtct
     2161 ttgcaaaact atatttcaac aaagtgtcat taagataact tgttaatgca ttaaaaaaaa
     2221 cagagtcgcc aagttgccag cgaagcatgt gtaacaaata ggagccttta ttataggtta
     2281 agcgtccatt aaatattctg cttacatcat tagtgtcagt acaaaatacc gaaccatcgg
     2341 gctgcgaggt tatattgttt atcttttgtt ttttccagtt ataccacaac gatggataat
     2401 aacgctcttt agttaatcct tcaaaataag ttgcaaaacc ttcgtttagc caaatatctt
     2461 cccagctacc acaagtaact aaatcaccaa accactgatg tgcacattca tgcgccatca
     2521 ggtcctgact aaagttaacc ataaaactca tggtttgatg ttccatgcca ccgccccagt
     2581 taaattgtgc atgaccatac ttttcatgac tgaaaggata aggaatagtg aggctgtcat
     2641 aaagttgaat gattggaatt atatcaggtg tttgtgtttg tgctgaagat aagttttcag
     2701 gataaacata attgagcact tgcaaagaat ctccaccggc cattggcaca tagtcggaat
     2761 atgcagcata attggtaact gcaattgcaa taagatag
//
LOCUS       DAOOOI010000180         2779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_6003_length_2779_cov_9.876285, whole genome shotgun sequence.
ACCESSION   DAOOOI010000180 DAOOOI010000000
VERSION     DAOOOI010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6003_length_2779_cov_9.876285"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="PKX59_11540"
     CDS             <1..1204
                     /locus_tag="PKX59_11540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91531.1"
                     /translation="LENPPIQTYNAPGDYVVTQTVTANGSPQYFLTDITVASIPDNYG
                     APVDVPDMYFYLYDSQGNQIYDSHPSVSNTNAPLSWTLPNIPLQNNNYTLHVWDEDGG
                     LFGADDDLGSVTFPGWSASGTATGTLSGVSGSLVVNYSIMQVPVATAIHTDTVHVYAV
                     PDTPMVSANIPLILCTGDTVVLTCNDTLQVQWYESGNLLIGETNSDLVVTASGNFSAV
                     VTNAHGCTATSVTISVQVNPNPPKPTFFVNGNTLNCVLTGYMLQWYFNGSALTGETAL
                     QLTATQQGTYSVIATDSISGCSSVSDPLVFTPVGIDQLQSVISAILFPNPADKSVVVQ
                     LTSGRNVFTTVQLLDISGWLLQQKDFMLQQGSTQLPFDLTDTSKGLYLIKITQKNASK
                     TLRLVISR"
     gene            complement(1208..>2779)
                     /locus_tag="PKX59_11545"
     CDS             complement(1208..>2779)
                     /locus_tag="PKX59_11545"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOZ91532.1"
                     /translation="VGAPPSSIGKFADETDNWMWPRQTGDFALFRIYADKNNNPADYS
                     NENLPYKPKYVLPISLNGIKENDFTMVYGFPGRTQEYLPMEAVRTLMNHTDPVRIELR
                     KIRLDQMEQAMEHNDTVRIQYSSKHSSIKNGYTKWQGEILGLKRLNALGVKSQREFDF
                     YQWAKKNSNTEYQKVVSELNQLHIKQRPLTIAADYYFEAIKGIELLTFSETFSRLEKI
                     CSTNPVDEKALSELKANLLKSTAGYFKNYDVAIDKAIMPLLLKEYIDKTPAGLEANLN
                     FIAKKHKNNISSYCDYVFKKTVFADQKRTESFIQSFTPNKLSKLLNDPAYTLIKQCSL
                     AYASKVLPEMTKMTLEINNLQKVYMKGQIEMAAENKKNIYPDANFTLRITYGKVEGYE
                     PRDGIAYKYYTTLDGVAEKYDSTNYDFNVPEKLLELHKKKDYGRYATDGKLVPCFLAS
                     NHTTGGNSGSPVLNGQGQLIGINFDRVWEGTMSDIMFDPAKCRNITLDIRYVLFIVDK
                     FAGAKWLTEEMNIVQ"
BASE COUNT          779 a          526 c          525 g          949 t
ORIGIN      
        1 actggaaaat ccaccgatac agacatataa cgcacctggc gattacgtag taacacagac
       61 tgttacagcc aatggttcac cacaatattt tctgactgat attaccgtag ccagtatccc
      121 cgacaattat ggtgcaccgg ttgatgttcc tgatatgtat ttttatctgt acgattctca
      181 aggcaatcag atttacgata gccatccttc tgtttcaaat acaaatgcac ccttatcttg
      241 gacattgccc aacatacctt tacaaaataa caattacacc ttgcatgtat gggatgaaga
      301 tggtggattg tttggtgcag atgatgattt gggctccgta acctttccgg gatggagtgc
      361 cagtggaaca gccacaggta cattgtctgg tgtttccggt tcgttagtag tgaattattc
      421 aataatgcag gtacctgttg ctacagctat tcatacagac actgtgcatg tttacgcagt
      481 tcctgataca ccgatggtaa gtgctaacat tccattgatt ttgtgtacag gcgatactgt
      541 tgtgttgact tgtaatgata cattgcaggt tcaatggtat gagagtggca acctgctgat
      601 tggggagacc aattcagact tagttgtaac tgcatcaggt aatttttctg ctgttgttac
      661 aaatgctcat ggttgcacag caacctcagt aactattagt gttcaggtga atcctaatcc
      721 accaaaacca acgttttttg tcaatggtaa tactttaaac tgcgtgctaa ccggttatat
      781 gttacagtgg tattttaatg gttcagcatt gaccggtgaa actgcattgc aattaacagc
      841 aactcaacaa ggcacttatt ctgtaattgc tactgatagc atttcaggat gcagctctgt
      901 atcagatcca ttagtgttta ctccggttgg aatagatcaa cttcaatctg tcatttcagc
      961 aatcttattt ccaaaccctg cagataaaag tgttgtagtg caacttacat caggtagaaa
     1021 tgtttttaca acagttcagc ttttagatat tagcggatgg ctgttgcaac aaaaagattt
     1081 tatgctgcaa caaggctcta ctcaactgcc attcgattta actgatacaa gtaaaggtct
     1141 ttatctgatt aaaataacac agaaaaatgc ctctaaaact ttgcggctag tgattagccg
     1201 ttgagtgtta ctgcacaata ttcatttctt cggttagcca tttggcacct gcaaatttgt
     1261 caacgataaa caaaacatag cgaatatcta atgtgatgtt tctgcatttt gcagggtcaa
     1321 acataatgtc gctcattgta ccttcccaaa cgcgatcgaa gttaattccg attaactgac
     1381 cttgtccatt gagaacagga cttcctgaat tacctcctgt tgtgtgattg gatgccagaa
     1441 aacaggggac taattttcca tctgtagcat aacggccata gtctttcttt ttatgaagtt
     1501 caagaagttt ttctggtaca ttaaaatcat agtttgttga atcgtacttt tctgcaactc
     1561 catcaagagt tgtatagtat ttgtaagcaa ttccatcacg tggctcataa ccttcaacct
     1621 tgccataggt aatgcgaagt gtaaagtttg catccggata gatgttcttt ttattctcag
     1681 ccgccatttc tatttgacct ttcatatata ctttttgcag attatttatt tctaaggtca
     1741 tctttgtcat ttcaggaagc acctttgaag cataagccaa tgaacattgt ttaatgagag
     1801 tatatgcagg gtcatttagg agtttactga gtttgtttgg tgtaaatgac tgaataaaac
     1861 tctctgttcg tttctgatct gcaaacacgg tttttttgaa tacataatca caataacttg
     1921 atatattgtt tttgtgtttt ttagcaataa aattaagatt ggcttcaaga cctgcaggag
     1981 ttttgtcaat atattcttta agtaataatg gcataatagc tttatcaatg gcaacatcat
     2041 aatttttaaa atagccggca gttgatttta gtaaatttgc ttttaattct gataatgctt
     2101 tttcatctac aggatttgta ctacagattt tttcaagcct tgaaaatgtt tcagaaaacg
     2161 taagtaattc aatgcctttt atggcttcaa aataatagtc ggctgcaatt gttagtggtc
     2221 gctgtttaat atgtagctga ttaagttcgc tcaccacttt ttgatattca gtgttagaat
     2281 tcttttttgc ccattggtag aaatcaaact ctcgttgcga ctttacacct aaagcattga
     2341 gtcttttcaa acctagtatt tcaccctgcc attttgtgta gccgtttttt attgatgaat
     2401 gtttggatga atattggatg cgaacggtgt cgttatgctc catagcctgt tccatttgat
     2461 ctaaccgaat tttacgaagt tcaatacgca cagggtcggt gtgattcatt aaggttcgta
     2521 ctgcctccat tggcaagtac tcttgtgttc gtccgggaaa accataaacc atagtaaaat
     2581 cattttcttt aattccattc agactaatag gcaaaacgta ctttggttta taaggcaaat
     2641 tctcattact gtaatcggca gggttgttgt ttttatctgc atatattctg aaaagagcga
     2701 aatcaccggt ctgacgtggc cacatccagt tatctgtttc atctgcaaac tttcctatgc
     2761 ttgatggcgg tgcaccaac
//
LOCUS       DAOOOI010000181         2766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_6032_length_2766_cov_10.406123, whole genome shotgun sequence.
ACCESSION   DAOOOI010000181 DAOOOI010000000
VERSION     DAOOOI010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6032_length_2766_cov_10.406123"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1661
                     /locus_tag="PKX59_11550"
     CDS             <1..1661
                     /locus_tag="PKX59_11550"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOZ91533.1"
                     /translation="LFVPVALLFKRKQVLIWCAVVIGSVSLLLFILDTFVYDLYRFHI
                     NGFVLDMVFGGAFSEIFVFDFLMYLKVAAFVVLLTAIVYLLARIIWSLFETDKLRGGK
                     QIAYLLVGLLIGSHLLHAWAYATSYRSIQQSSLYFPLYFPLRANKLLSKLGVAADNQK
                     QEWENLQDGNDKAIQYPLRPLTFDTVTKYPNILFILMDAWQFETFNNEVTPNIFRFSE
                     GKILFNRHYSGSNGTRGGIFSMFYGIPSLYWEDFLINRISPVFLDELQKRNYEMKILA
                     SAALVSPEFDKTVFSKINNLRTHTPGKNFTERDENITKDFIEWMNTSAFQKPFFGFLF
                     YDSPHNYNLPYWLPHPFKPYWENVDHTVLNNDFDPVPFYNSYKNTVWFCDSLIQQVLS
                     KLEEKNLLENTIVVITSDHGQEFNENKKNYWGHTSNFSKYQLQVPCIISWPGKNAEAI
                     SRFTSHYDLVPTLMQDVLKCRNLVSDYSFGNNLFDTTEQRVFVAGSKEHFAFIDLQKN
                     QINTVNFNGSMTVTDMQLNELNVKPDAKMLNDCVLDLQRYYKPH"
     gene            complement(1664..2563)
                     /locus_tag="PKX59_11555"
     CDS             complement(1664..2563)
                     /locus_tag="PKX59_11555"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HOZ91534.1"
                     /translation="MIKPTSFGFNIETSKDNVFQKQNNRLSNQQIQLEAEKESRLLRD
                     KLIDSNINVIYAYENCEHNTPDAVFPNNWFSTHDNDTLVLYPMLAANRRNERNAALIN
                     YLKNSFQIKEVVDMTPFENENKFLEGTGSLVFDHKNKICFASISERTNTELANNINKK
                     LGYQTIILNYTDENNTPVYHTNVCMCIGNGFVIAAIDNLKSKSERTALFEYFETNNLN
                     PILISQQQVYSFCGNALQLQTNTERPVLTMSTTAFNAFSTEQIKTIEKHSDILYSSID
                     TIESIGGGSVRCMLAEIFLNPKS"
     gene            complement(2593..>2766)
                     /locus_tag="PKX59_11560"
     CDS             complement(2593..>2766)
                     /locus_tag="PKX59_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOZ91535.1"
                     /translation="TTDPIVSVDIIGNTHSCIFDSMLTSVLGKMVKVVGWYDNEMGYS
                     TRLAELTALVGKQ"
BASE COUNT          857 a          431 c          488 g          990 t
ORIGIN      
        1 tactgtttgt tcctgtagca ttacttttta agaggaaaca ggttttaatt tggtgtgctg
       61 tagtaattgg ttcagtttct ttattactat tcattttaga tacttttgtc tatgaccttt
      121 acaggtttca tatcaatggt tttgttttgg atatggtttt tggcggtgct ttctcagaaa
      181 ttttcgtttt tgatttttta atgtacttaa aggtagctgc atttgtagtt ttactcaccg
      241 caattgttta tttgcttgca cgaattatat ggagtttatt tgaaacagac aaactgagag
      301 gaggaaaaca aattgcttat ttgttagttg gacttttaat aggcagccat ctgcttcatg
      361 catgggctta tgcaacttct tatcgctcaa ttcaacaatc atcactttat tttccattgt
      421 attttccatt gcgtgccaat aaacttctca gtaaacttgg agttgcagct gataatcaaa
      481 aacaagaatg ggaaaatttg caagatggaa atgacaaagc aattcaatat ccattaagac
      541 cactaacatt tgatactgta actaaatatc ctaacatatt atttatttta atggatgcct
      601 ggcagtttga aacttttaat aatgaagtaa ccccaaatat ttttcgtttc tcagaaggaa
      661 aaatcctatt taaccggcac tacagcggaa gcaatggaac tcgtggcggc atcttttcta
      721 tgttttatgg aataccgtct ttatattggg aagattttct gattaacaga atcagcccgg
      781 tttttttgga tgagttacag aaaagaaatt atgaaatgaa gattcttgct agtgcagcct
      841 tggtaagtcc tgagtttgat aagactgttt tttcaaaaat taataacttg cgtacacaca
      901 ctccgggaaa aaattttaca gagcgtgatg aaaatataac caaagacttt attgagtgga
      961 tgaatacatc tgcttttcaa aaaccatttt ttggtttttt gttttatgac tcaccacaca
     1021 attataattt accttactgg ttgcctcacc cttttaaacc ttattgggaa aatgtagatc
     1081 ataccgtgct taacaatgat tttgatcctg ttccatttta taacagttat aaaaatacag
     1141 tttggttttg tgattcattg attcaacaag tattgtctaa acttgaagaa aagaatttgt
     1201 tggagaatac cattgttgta attacctcag atcatggaca ggagtttaat gaaaacaaga
     1261 aaaattattg gggacatacc agcaattttt ccaaatacca acttcaggtt ccttgtataa
     1321 tatcatggcc gggaaaaaat gctgaagcaa taagtaggtt tacatcccat tatgatttag
     1381 tccctacact aatgcaagat gtattaaagt gcagaaattt agtttcggat tattcattcg
     1441 gtaataattt gtttgataca acagaacaac gggtttttgt tgccggaagt aaggagcatt
     1501 ttgcatttat tgatttgcag aaaaatcaga ttaatacagt taattttaat ggttcaatga
     1561 cggttacaga tatgcaattg aatgaattaa atgttaaacc tgatgcaaaa atgctgaacg
     1621 attgtgtttt ggacttgcaa cgatactaca agccacatta gaatcagctt ttagggttaa
     1681 gaaaaatttc tgccagcatg caacgtacac taccaccacc aattgattca atagtatcaa
     1741 tggaggagta taggatgtct gaatgttttt caatagtttt tatttgttct gttgaaaatg
     1801 cattaaatgc agtagttgac attgtaagta ctgggcgttc agtattagtt tgaagttgta
     1861 aagcattgcc acagaaagaa tacacctgtt gttgtgaaat cagaatgggg ttcagattat
     1921 tagtttcaaa atattcaaac agtgcagtac gttctgattt tgatttcaga ttatcaattg
     1981 ctgcaataac aaaaccgtta ccaatgcaca tacatacatt agtatgatag actggtgtgt
     2041 tgttttcatc agtgtaattc agtattattg tttgataacc taattttttg ttgatattat
     2101 ttgccaactc agtatttgtt ctttctgaga ttgaagcaaa acaaattttg tttttgtgat
     2161 caaatactaa gctgcctgtt ccttctaaaa atttattttc attttcgaat ggagtcatgt
     2221 caacgacctc tttaatttga aaagaattct ttaaataatt tataagtgca gcattccgtt
     2281 cgtttcttct gttagcagca agcattggat aaagtaccaa agtatcatta tcatgagtac
     2341 taaaccaatt gttcggaaac acagcatcag gtgtgttatg ttcgcagttt tcataggcat
     2401 aaatcacatt gatgttgcta tcaattaatt tatctcttaa caaacgactt tccttttcag
     2461 cttcaagctg tatttgctga ttgcttaatc tgttattttg tttctgaaag acattgtctt
     2521 tgcttgtttc tatgttaaaa ccaaaagaag ttggctttat cataagaaca gttgatgcaa
     2581 agtgcatatt gtttattgct tgccaactaa tgctgtcagt tcagctaaac gtgtgctgta
     2641 gcccatttca ttatcgtacc agccaacaac tttaaccatt ttaccaagta cagatgtgag
     2701 cattgaatca aaaatgcatg aatgtgtatt gccgataatg tcaacagaca ctattgggtc
     2761 ggttgt
//
LOCUS       DAOOOI010000182         2760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_6049_length_2760_cov_5.043623, whole genome shotgun sequence.
ACCESSION   DAOOOI010000182 DAOOOI010000000
VERSION     DAOOOI010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6049_length_2760_cov_5.043623"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="PKX59_11565"
     CDS             complement(<1..946)
                     /locus_tag="PKX59_11565"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOZ91536.1"
                     /translation="MRKFFYLVLLGSIFSTTVFSQGIVSGVVKDKNHKPVAYANVYVK
                     PGFVGTTTSSDGIFEIELVPGKYTLYCQNLGYKQFSKQIEVGAGKQQIEIELTDELYS
                     LKEVAVTNNKEDPAYEYIRKTIAKRKSYLNEVDAYSCNVYIKGVQKLNKYPEKFLGKV
                     VDFGGVVDSTTGIFYLSESVSKYSFEKPNKIREEMISSRISGNNRAFSFNKASDMQFN
                     FYENRVMSEGISSKGFVSPVAGDALFYYRYKLIGSVVENDRLIHKIEVIPRRQNDPVF
                     RGVIYIADDLWRFQFVDLFLTRDAGIEFLDTLRIRQSFV"
     gene            complement(1002..1928)
                     /locus_tag="PKX59_11570"
     CDS             complement(1002..1928)
                     /locus_tag="PKX59_11570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOZ91537.1"
                     /translation="MVSISVVVPTYNRLNFVVKTIESLLNQQSDDFEILIVDDGSTDK
                     TEEYFSTYKHPKVTYHRIVNSERGFARNYGASIAKGRFINFFDSDDLAYNNHISTALN
                     YIAKFDNPEIFHLNYVYQTLDGQDLDVLKADSDNIGEMLIKKGNILSCNSVVIRKDIA
                     EKFPFCESRLLSGTEDYALWLALSSRFKIHYVPEITSVVIDHEQRSMASHDIIKMINR
                     NMFLLDHLQADQHFMQYVGKRFNQIQCECFSFIALHLMLEGKNKDGIYFLSKALIADW
                     RFPFRRIRFMAIMKHMLKSFFRIQRSKVPEAV"
     gene            2061..2630
                     /locus_tag="PKX59_11575"
     CDS             2061..2630
                     /locus_tag="PKX59_11575"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOZ91538.1"
                     /translation="MNHRPTEFTERKKGAIEVICGSMFSGKTEELIRRLKRARIAKLK
                     VEIFKPSVDQRYHESNIVSHDSNYIPSSPAQSSSQILLLTNDVDVVGIDEAQFFDLEL
                     PNVCLKLAHQGIRVIIAGLDMDFKGEPFGPIPRLMAIADFVTKVHAICMDCGDLALFS
                     YRTVPDESLIVLGEKESYIPLCRTCYHKR"
BASE COUNT          925 a          539 c          434 g          862 t
ORIGIN      
        1 ggacaaaaga ctgccttatt ctgagtgtat ctaaaaactc aatacctgca tcgcgtgtaa
       61 ggaaaagatc aacaaactga aatcgccata aatcatctgc aatataaata acacctctga
      121 acaccggatc gttctgtctt ctggggatta cctcaatttt atgaataagt ctgtcgttct
      181 ctacaactga accaattaat ttataacgat aataaaacaa agcatcgccc gcaactggtg
      241 agacaaatcc tttggaagaa atgccttctg acataaccct gttttcataa aaattaaatt
      301 gcatatcaga tgctttatta aagctgaatg ccctattatt accacttatg cgcgaagaaa
      361 tcatctcttc acgaatctta ttcggttttt cgaaactgta ttttgaaacc gattcactta
      421 aataaaatat gcctgttgtt gagtcaacca ctccaccaaa atctacaacc ttaccaagaa
      481 atttttcagg atacttattt aatttctgta cacctttgat ataaacattg cacgaatatg
      541 catcaacttc gttcagataa cttttccttt tagcaattgt ttttctgata tattcatagg
      601 ctgggtcttc tttattgttt gtaacagcaa cctcctttaa cgaataaagc tcatctgtaa
      661 gttctatctc tatttgttgt ttacctgcac caacttcaat ctgtttgctg aattgcttat
      721 agcccaggtt ctgacaataa agagtgtact taccgggaac taattcaatt tcaaaaatac
      781 cgtcactact tgttgtcgtc ccaacaaaac cgggtttcac atatacattg gcataagcta
      841 caggtttatg atttttatcc ttcactactc ctgatacaat accctgagaa aacactgtcg
      901 tactaaaaat actgcctaac agaaccaggt aaaaaaattt acgcatcaca acaattaatt
      961 cttaatttca ggatgctaat gtaaaacttt aaatttgggt ttcagacagc ttccggaact
     1021 ttgcttctct gaatgcggaa gaatgactta agcatgtgtt tcataatagc cataaaacga
     1081 attctacgga acggaaaacg ccagtccgct attaaagcct ttgaaagaaa ataaatacca
     1141 tctttatttt ttccttccag catgaggtgc aatgctataa aagaaaaaca ttcacattga
     1201 atctgattaa atcgtttacc aacatattgc ataaaatgtt gatcagcttg aagatgatct
     1261 aagagaaaca tgtttctgtt aatcatcttt ataatatcgt gcgaagccat actacgttgt
     1321 tcatggtcaa ttactacact ggtaatttcc ggcacataat gtattttgaa ccgtgaggac
     1381 aaggctagcc acaaagcata atcttcagtg cctgaaagaa gacggctttc acaaaatgga
     1441 aacttttcag caatatcttt tctgattact acagaattac aacttaaaat attacctttt
     1501 tttatgagca tttcaccgat attatcagaa tctgctttca ggacatctaa atcctgtcca
     1561 tcaagtgttt gataaacgta attaaggtga aaaatttcag ggttatcaaa cttcgcaata
     1621 taattcagtg cagtagaaat atgattatta taagctaaat catctgaatc aaaaaaatta
     1681 ataaacctac ctttagcaat tgatgctcca tagttacgtg caaatccacg ctcgctgttt
     1741 acaattctat ggtaggttac ctttggatgt ttataggttg aaaaatattc ctctgtctta
     1801 tcggtactac catcatcaac aatcaaaatt tcaaaatcat ctgactgttg attcagcaat
     1861 gactcaatcg tttttacaac aaaattcaac ctattataag ttgggactac tacagaaatg
     1921 gaaaccatga gaaaaagtat tgcaaaacta cagtacaata actgaaaaca aacatttctt
     1981 aaaaaaaaat tatccgctaa atgttaaccg gctaaaaatt gcagcaaaca gattttacct
     2041 atttttgaaa ccgttaagat atgaaccata gaccaacaga atttacagag cgaaaaaaag
     2101 gcgctattga agtcatttgc ggcagcatgt ttagtggtaa gacagaggag ttgattcgca
     2161 gactaaaacg agcgcgcatt gccaagctta aggttgaaat atttaaacct tctgttgatc
     2221 aacgctatca tgaaagcaac attgtttcgc acgattcaaa ctatattcca tcatcccctg
     2281 cccaatcatc ctctcaaatt cttttactca caaacgatgt tgatgttgta ggcattgacg
     2341 aagcgcaatt ttttgatttg gaactgccta acgtttgcct taaactggca catcagggaa
     2401 tcagggtaat tattgcaggt ttagatatgg actttaaagg cgaacctttc ggacctattc
     2461 caagacttat ggcaatagca gactttgtta caaaggttca tgccatctgt atggattgtg
     2521 gcgaccttgc cttattttct tatcgtaccg tacccgatga atcactcatt gttctgggtg
     2581 aaaaagaaag ctacattcct ttgtgcagaa cttgttatca taaaaggtaa ttgttaccaa
     2641 tttgatgcac tatcaaagct taattttgta ggctattcaa tttaaaaata tgtacaccat
     2701 tacagaggca gcgtcaatat taaaaggaac gctggttttg aatgtaaacg ataatcagac
//
LOCUS       DAOOOI010000183         2731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_6128_length_2731_cov_10.607997, whole genome shotgun sequence.
ACCESSION   DAOOOI010000183 DAOOOI010000000
VERSION     DAOOOI010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6128_length_2731_cov_10.607997"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..193)
                     /locus_tag="PKX59_11580"
     tRNA            complement(119..193)
                     /locus_tag="PKX59_11580"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Val,seq:tac)
     gene            complement(322..1089)
                     /locus_tag="PKX59_11585"
     CDS             complement(322..1089)
                     /locus_tag="PKX59_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOZ91539.1"
                     /translation="MPKKGVHDASAEEKLQALFQLQQIDSEIDRIQIVRGELPVEVKD
                     LEDDVARLETRISNINNDMAEFDRMIAERKQGIKDSTAAIKKYEGQQNNVKNNREYES
                     LAKEIEFQKLEIQLCEKRIKEYTFEITSKKQILTESEEDLTHRKADLENKRKELDSII
                     AETRKEEDVLNEYSGKAAAIIEERLLSGYKKIRENAANGLAVVSIQRDSCGGCFNKVP
                     PQRQLEIKQRKKVIVCEHCGRILVDQEIMTDLPENPA"
     gene            1283..>1527
                     /locus_tag="PKX59_11590"
     CDS             1283..>1527
                     /locus_tag="PKX59_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91540.1"
                     /translation="MPVLVFFTSLDDVTFVFVSIGLAFILFFSFSVALVFVVFGAAAF
                     LAGFVGVAFLAGAFFAAGFLAAGFATTFFLAGAFFTAA"
     assembly_gap    1528..1537
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1604..2698)
                     /locus_tag="PKX59_11595"
     CDS             complement(1604..2698)
                     /locus_tag="PKX59_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOZ91541.1"
                     /translation="MRINEVINVLENWAPPAYQESYDNCGLLTGNSADEVKGIMVCLD
                     TTEAVLDEAIKENCNLIIAHHPIVFSGLKKITGRNYVERVIIKAIQNNLALYALHTNL
                     DNIDSGVNQRLCHQLGLENAKILLPKQGTLKKLVTFTPIDQAEKVRTTLFAAGAGNIS
                     NYSECSFNIEGKGTFKPEAEANPVIGEKLIRHIESEVRTEIIYESHKEHKVLKALFES
                     HPYEEVAYDIYALTNSHQQTGSGMIALLKDEMDEMVFLEMIKSKLNCSVLKHTNLCHK
                     KIRKVAVCGGSGFFLLPQAIAAGADAFITSDIKYHQFFDADNRILLIDAGHYETEQFT
                     MMLIKDLLIKNFTTFAIRLTKVDTNPVKYI"
BASE COUNT          673 a          544 c          473 g         1031 t
ORIGIN      
        1 aaataagcct tttattgttg ttgttcgaag tcattccttt gtagaagaca ttgtgtgatt
       61 gcgaatagat aacgtaaaca gtgaacataa ataaaaaagt ccgcatttgc ggacttcgtg
      121 ggagcaacag gattcgaacc tgtgaccctc tgcttgtaag gcagatgctc tgaaccagct
      181 gagctatgct ccctaactat ttttaatggg tcgttttttt atgagcgtgc aaagatacag
      241 ataggtaagt attctccaaa tttattttga aaatatggca taaaaaaacc gcttttaagc
      301 ggctttttta ttgttatcta tttatgcagg attttccggc aagtccgtca taatttcctg
      361 atctacaaga atacgaccgc agtgttcaca tacaatgact ttcttacgtt gcttaatctc
      421 caactgacgt tgtggtggaa ctttattaaa acaaccacca cagctatctc gctgaataga
      481 aactacagcc aggccattag ccgcgttctc acgaatcttt ttatatccgc tcaacaaacg
      541 ctcttcaata attgcagcag cctttcctga atattcgttc agaacgtctt cttccttacg
      601 ggtttctgca ataatgctgt ctagttcttt gcgtttgttt tccaaatcag ctttgcgatg
      661 cgttaaatcc tcttctgatt ctgtaaggat ttgtttttta gatgtgattt caaaagtata
      721 ttctttaatt cgcttctcac aaagttgtat ttcaagtttc tgaaactcta tctcttttgc
      781 aagcgattca tactctctgt tattcttcac attattttgc tgcccctcat atttctttat
      841 agctgcagta gaatctttaa tgccttgctt tctttcagca atcattctgt cgaactctgc
      901 catatcatta ttgatattgc tgattcttgt ttcaagacgt gctacatcat cttcaagatc
      961 ttttacttca acaggtaatt cgcctcttac aatctgaatt ctgtcaatct cagagtcaat
     1021 ttgttgaagt tgaaacaatg cctgaagctt ttcttctgca gaggcatcat gaacaccttt
     1081 cttaggcatt tttatctgaa actgagatat gtcgccaaca ctgtatttgc ttgataattt
     1141 tgctgccgta cctgtatcaa tagcttttaa gttacgtgtt tccgttaagc gtttaactac
     1201 cggtttgctc accttggtaa ttaccggttc ttctaccttc ttacttctgc tttttgtttt
     1261 tgctttagtg tctgtttttg acatgcctgt acttgttttt tttacttctt tggatgatgt
     1321 gacttttgta tttgtttcta ttggtttggc ctttatactt ttcttctctt tttcagtagc
     1381 tttagttttt gtggtctttg gtgcagcagc ctttttagct ggttttgtag gagttgcttt
     1441 cttagccgga gctttttttg cagcaggttt tttagctgcc ggttttgcaa caactttttt
     1501 cttagcagga gcttttttca ctgctgcnnn nnnnnnntct tagctgctgg ctttgcactt
     1561 gttttttttg ctgcagcttt cacaggtttt tttgccgcca taatcagata tattttaccg
     1621 gattagtatc cacttttgtt aaacggatgg caaatgtagt aaaatttttg attaacaaat
     1681 ctttaatcag catcattgta aattgctccg tctcgtaatg tccggcatca atgagtaaaa
     1741 ttctgttatc ggcatcaaaa aactgatggt attttatgtc tgatgtgata aaagcatctg
     1801 caccggctgc aattgcttgt ggcaaaagaa aaaagcctga accgccacag acggcaactt
     1861 tccttatttt tttatggcat aagttggtgt gttttaatac actgcaattc agctttgatt
     1921 taatcatttc caaaaagacc atttcgtcca tctcatcttt aagcagggct atcatccctg
     1981 atcctgtctg ttgatgactg ttggttaatg cataaatatc ataagcaact tcctcatagg
     2041 gatgtgattc aaacaaagct ttcagcacct tatgttcttt atgtgattcg taaataattt
     2101 ctgtacgaac ttcactctca atgtgtctga ttaacttttc tccaataacg ggattggctt
     2161 cggcttctgg cttaaatgtg cctttgccct caatattaaa actacattcg ctgtaattgc
     2221 tgatgtttcc cgcaccggct gcaaaaagtg tagttctgac tttttctgcc tgatctattg
     2281 gagtaaaagt tactaacttc tttaaagtgc cttgtttcgg caataatatt tttgcgtttt
     2341 caagacccaa ctgatggcat aatcgctgat ttactccaga atcaatattg tctaaattgg
     2401 tatgtaatgc ataaagcgca aggttgtttt gtattgcttt tatgattaca cgctcaacat
     2461 aatttctacc ggtaattttt tttaatccac tgaaaactat cggatgatgt gctataatca
     2521 aattgcagtt ttcttttatt gcttcatcga gtacagcttc agtagtgtca aggcaaacca
     2581 ttattccttt tacctcatca gcagaattgc cggttaaaag tccgcagtta tcataacttt
     2641 cctgatatgc cggaggtgcc cagttctcta atacatttat gacttcattg attctgatca
     2701 ttgctttttc tgccttgcaa aagtaagcat g
//
LOCUS       DAOOOI010000184         2725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_6140_length_2725_cov_9.689139, whole genome shotgun sequence.
ACCESSION   DAOOOI010000184 DAOOOI010000000
VERSION     DAOOOI010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6140_length_2725_cov_9.689139"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PKX59_11600"
     CDS             complement(<1..331)
                     /locus_tag="PKX59_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008623230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ91542.1"
                     /translation="MQTKILIVDDERDVELLFRLKFRREIKEKHIDLHFAFSGEDALN
                     YLHNINPMDVLLVLSDINMPGMNGLQLVEKVKAEFPAMRIIMVSAYGDDKNYNEAKAK
                     GVDDFVTK"
     gene            complement(342..>2725)
                     /locus_tag="PKX59_11605"
     CDS             complement(342..>2725)
                     /locus_tag="PKX59_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOZ91543.1"
                     /translation="NSIWTIYRDKAGIIWIGTDNGLDYYKEDLMRFKTELPLPDDPLS
                     NATPNKNVFCFLPEGNNLWMGVLGTGVVVKESTGTIKQVYNAANGLADNSVFCLIKDR
                     NGIVWVGTYNGLTSINPQSGVVRSYRIADGLTNSNIRSLAEDENGNIYIGTYGGGLCV
                     LNRQTDKISSIRKEDGKGLTNNIITALKYDGNGNLFIGTYGGGLCLLNISANTITAFE
                     SKPGSNTTISSNYVNSIYSFSNQQLLVGTYSGGINIFNYATKSFTALTETNGVPNNNV
                     TNIVSDAEKNIWFTTGNQICKMKLAPSDSILTMRIYDEQDGVFNRFNPGAFAQTADGK
                     IYFGGISGINRFLPSAIRDNPYIPPVVITKFYLFEKPYFMDTIIMSKNMIELTYNQNF
                     FSFEYAALNYILPEKNQYAYMLEGLEKTWTYCGSKRDRQYTDLDPGTYKFRVKACNND
                     GIWNQDGTYIIIIIKPPFWKTWWFYILSGLTFVGLTITYIRWRTRTLMKQYEILEQKV
                     QERTAELREEKEKSEQKSLEIERALKELKDTQSQLIHAEKMASLGQLTAGIAHEIQNP
                     LNFVNNFSELSQDLIDELNATDDKEEWKSISHDIKTNLEKINEHGKRAERIVKSMLMH
                     SRSQTAEKVLTDINKLAEDVMNLAYTSLRANDSNFGCKNMIELDASLPKLSIVQQDIS
                     RVLLNIFNNAFYAVNQLRKKSDKAFHPEVRLRTWKAANKVYISISDNGGGIPDDIVDK
                     IFQPFFTTKPTGEGTGLGLSLSYDIITKGHYGMLSVNVQKGIGSEFVIELPQL"
BASE COUNT          806 a          553 c          441 g          925 t
ORIGIN      
        1 gcttagttac aaaatcatct acacctttcg ccttagcttc attataattc ttatcatcac
       61 cataagctga caccataata attcgcattg caggaaattc agcctttact ttttcaacca
      121 actgaagtcc gttcatgccc ggcatattta tatccgacaa taccaataaa acatccatag
      181 ggttaatgtt gtgaagatag tttaaagcgt cttcaccact aaatgcaaag tgaagatcaa
      241 tatgtttctc tttgatttca cgtctgaatt ttagcctgaa caacagttca acatcacgtt
      301 catcatctac tatcagaatt tttgtttgca tgatattttt attatagttg gggtaattca
      361 ataacaaact cagaccctat acctttctgc acattcacac ttagcatacc ataatgacct
      421 ttggtgatga tatcataact taatgataaa cccaatccag ttccttcacc ggtaggctta
      481 gttgtaaaga atggttggaa gattttatca acgatatcat caggaattcc accaccatta
      541 tcactgatac taatataaac tttgtttgca gctttccatg ttctaagtcg aacttcagga
      601 tgaaatgctt tatcagattt tttacgtagt tgatttacag cataaaatgc attgttaaaa
      661 atattgagta acacgcgact gatatcctgc tgaactatgc tgagctttgg caatgaggca
      721 tccaactcaa tcatattctt acacccaaaa ttactatcgt ttgctcgaag gcttgtatag
      781 gcaaggttca ttacatcctc tgcaagtttg ttgatgtctg ttaaaacttt ctctgctgtt
      841 tgcgaacggc tgtgcatgag catactcttc actatacgct ctgcacgttt accatgctca
      901 ttaatttttt cgagattggt ttttatatca tgagaaatag acttccattc ttctttgtca
      961 tcagtagcat tcaactcatc aataagatct tgagataact ccgaaaaatt gttaacgaag
     1021 ttaagtggat tttgaatttc atgagcaata cctgcagtaa gttgacctaa agaagccatt
     1081 ttctctgcat gtatcagttg cgattgtgtg tctttaagtt cctttaatgc gcgctcaatt
     1141 tctaatgact tctgttctga tttttctttt tcttctcgca attctgctgt gcgctcttgt
     1201 actttctgtt ccaaaatttc atattgtttc atcagcgttc gtgtacgcca acgaatgtaa
     1261 gttatggtta aacctacgaa agtaagaccg gataaaatat agaaccacca ggttttccaa
     1321 aacggtggtt tgatgatgat gataatatat gttccatcct gattccagat tccatcgttg
     1381 ttacatgctt taactctgaa cttatacgtg ccggggtcaa gatctgtata ttggcgatcg
     1441 cgtttactgc cacaatatgt ccacgtcttt tcaagtcctt caagcatgta ggcatattga
     1501 tttttttccg gtagaatgta atttaaagct gcatattcaa atgaaaaaaa gttttgattg
     1561 taagttaact ctatcatatt tttggacatg ataatcgtgt ccatgaaata aggtttttca
     1621 aaaagataaa attttgtgat aactacagga ggtatgtaag gattatcgcg aatagctgat
     1681 ggcaagaaac gattaattcc actgatgccc ccaaagtaaa ttttgccatc tgcagtttgt
     1741 gcaaatgcac ccggattgaa gcgattaaaa acaccatctt gctcatcata aattctcatg
     1801 gtgagaatag aatctgaagg agccagtttc attttgcaga tttgatttcc tgtagtaaac
     1861 cagatatttt tttctgcatc tgaaacaata ttcgtcacat tattattggg aacaccattg
     1921 gtttcagtca atgcagtaaa actttttgtg gcataattaa aaatatttat tccaccactg
     1981 tatgtgccaa caagcaattg ctgatttgaa aaactgtata ttgagttgac ataattactg
     2041 ctaattgtgg tattgcttcc gggctttgat tcaaatgctg ttatggtatt tgcagaaatg
     2101 ttcaacaaac ataatccacc accataggtg ccaataaaca aatttccatt gccatcatat
     2161 tttaaagccg ttattatatt attcgttaaa cctttgccat cttctttacg gatacttgaa
     2221 atcttatccg tttgtctatt aagtacgcaa agcccaccac cgtaggtgcc aatataaata
     2281 tttccatttt catcttcggc aagcgatctg atattactat tggtcaaacc gtctgctatc
     2341 cgatagctac gcacaactcc tgactgtggg ttgatggacg ttaaaccatt ataagttcca
     2401 acccaaacaa taccatttct atctttgatt aaacagaaca cactgttgtc tgcaagtccg
     2461 tttgcagcat tatatacttg tttgattgtg cctgttgatt ctttaacaac cacacctgtt
     2521 cccaaaacac ccatccacaa attattcccc tcaggcagaa aacaaaaaac attcttattg
     2581 ggagtcgcat tactcaatgg atcatccggc agtggcaact ctgttttaaa acgcatcagg
     2641 tcttccttgt aataatcaag accattgtct gttccaatcc agattatgcc tgccttatca
     2701 cgatatattg tccaaatgct attgc
//
LOCUS       DAOOOI010000185         2660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_6298_length_2660_cov_16.920921, whole genome shotgun sequence.
ACCESSION   DAOOOI010000185 DAOOOI010000000
VERSION     DAOOOI010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6298_length_2660_cov_16.920921"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..>2660
                     /locus_tag="PKX59_11610"
     CDS             325..>2660
                     /locus_tag="PKX59_11610"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOZ91544.1"
                     /translation="MKHYYFTFILIIFLSLNHPLKAQVLPPQFTDQLVSTGWDFVEGY
                     TEDSTGQKYVWEKAGLVWVVDTNGVKIPQPLIDISEEVGNWRDHGMNGFAIDPNFRTN
                     GYFYLYYVVDRHYLLHYGTGSYSPTTNEYDAATICRITRYTANSATNFTTTVPGSRLI
                     LLGETKKTGIPVLYDTHSAGTLLFATDGTLIVSAGDGASPATADSGQVASILSYWAQA
                     LADSIITPQQNVGAYRAQLIQSLNGKILRLDPATGDGIPSNPFYDSTDARSPQSRVWA
                     LGLRNPFRISLKPGTGESDPTAGNPGTFYVGDVGWQFWEDINICNSPGMNFGWPVYQG
                     YDLDPNYFAAKQFNFFAPNPLYGSGGCTQPYFTFHELVKNPTANGIVSFDNSCNPGQP
                     IPATVHTFIHARPVIDYYHGNRSRTGIFTGNNASTIDIDDPLSPVDGVRFGGFASVAG
                     PLYQGTKFPLDLQGSYFHADYGGEWIKQFKFNANDTATYVRDFASNMGPVVFLTENKK
                     DGCLNYVKYPDQIRKICYVGNVNNIPIAVALSDTTYGASPLVVQFNGSQSHDPENDSL
                     SFFWSFGDGFTSTLISPQHTYNSPGGVIQSYWAVLTVTDTAGNSAKDSVFISINNTPP
                     VVNITSFNDGDLYSMLQISTLPLQATVTDAEHVPGQLHYKWNVYFHHNQHEHPEPTDT
                     NKITSATILPAGCDGEIYYYRIRLTVTDDGGLSGYSEKSVYPNCTTDCNGVFNGTAFI
                     DSCNTCVGGNTGLQPCTQDCHGDWGGTAFIDSCSTCVGG"
BASE COUNT          812 a          501 c          500 g          847 t
ORIGIN      
        1 tttactttga gcttgtactg ttatattgcc agaaaaaaat acaactgcaa acatgactac
       61 agatgaggta aatgttttct tcatttccat taaatattta aataatacat gagttttgaa
      121 cttcctttta tactgacctt ctaaaaaaag gttgcattta cttctattgt acacttatta
      181 aaaaacgaat ttatcgttga ctaagaataa tttaacattc ttcatactca tggattgcaa
      241 gtggaaaact taaagagatt ttacatgcct gatatttttc agtcatttat atttgctact
      301 gctttttcgt tataaaccga catcatgaaa cattactact ttacttttat attaattatt
      361 tttctttcac tcaatcatcc attaaaagca caggtacttc ctccacaatt tactgatcag
      421 cttgtcagca caggctggga ctttgtagaa ggttatactg aagattcaac aggacaaaaa
      481 tatgtttggg aaaaagcagg attagtttgg gttgttgata ccaatggcgt taaaatacca
      541 caacctttaa ttgacattag tgaagaagta ggtaattggc gcgatcatgg aatgaatggt
      601 tttgcaattg accccaattt cagaactaac ggttactttt atttatacta tgttgttgac
      661 aggcactatc tcctacacta tggcacaggg agctatagcc caacaacaaa tgaatatgat
      721 gcagctacta tttgtcgcat aacaagatat acagcaaatt ccgcaacaaa ctttacaaca
      781 acagtacccg gaagcagact cattttacta ggtgagacta agaaaacagg aatccctgtt
      841 ttgtatgaca cacatagtgc tggaacatta ttgttcgcta ctgacggaac attgattgta
      901 tcggccggtg acggtgcttc gcctgctaca gcagatagcg gacaagttgc ttctattctt
      961 agttactggg cgcaggcatt agcagatagt atcatcacac cacaacaaaa tgttggtgct
     1021 tatcgggcac aactaattca atccttgaat ggaaaaatat tacgattaga tcctgcaaca
     1081 ggtgatggaa tcccttcaaa tccattttat gacagtaccg atgcacgttc tcctcaatca
     1141 agagtttggg cattggggtt aagaaatcca tttcgtattt cacttaaacc ggggacagga
     1201 gaatcagacc ccacagcagg taaccctgga acattttatg ttggtgatgt aggttggcaa
     1261 ttttgggaag acattaatat ctgcaatagc cccggaatga attttggatg gccggtatat
     1321 caaggttatg atttagatcc aaattatttt gctgcaaaac aatttaactt ctttgcacca
     1381 aatccacttt atggttcagg tggttgcaca cagccctatt ttacttttca tgaattggtt
     1441 aaaaatccca cggctaatgg cattgtaagt tttgacaact cctgtaatcc gggacaacca
     1501 attcctgcaa cagtacatac tttcatccat gcacgacctg ttattgatta ttatcatgga
     1561 aatcgttcac gaacaggaat atttacagga aacaatgctt ctacaataga cattgatgat
     1621 cctttatcac cggtagatgg cgttcgtttt ggaggatttg cttctgttgc aggaccactt
     1681 tatcagggaa ctaaatttcc tctggatttg caaggaagct attttcatgc tgactacggt
     1741 ggcgaatgga ttaagcaatt taaatttaat gcaaacgaca ctgctaccta tgtaagagat
     1801 tttgcttcaa atatgggacc tgtagttttt ctgactgaaa ataaaaagga tggctgtctc
     1861 aattatgtaa aatatccgga tcaaattaga aaaatatgtt atgttggtaa tgtaaataac
     1921 atcccgatag ctgttgcatt atctgacaca acgtatggtg catcaccttt agttgttcag
     1981 tttaatggaa gtcagtcaca cgatccggaa aatgattctt tatctttctt ctggagtttt
     2041 ggtgatggct ttacatctac tctgataagt ccacaacata cttataatag tccgggcggt
     2101 gttattcaaa gctattgggc tgtattaacc gttacagaca cagctggtaa ttctgcaaaa
     2161 gattctgttt ttatttcaat aaataataca cctcctgttg taaacatcac aagttttaat
     2221 gatggtgact tgtattccat gcttcagatt tcaactttgc cacttcaggc aacagtgaca
     2281 gatgctgaac atgtacctgg tcaactacat tataaatgga atgtttattt tcatcacaac
     2341 caacacgaac atccggaacc tactgacaca aacaaaataa cttctgcaac tattttacct
     2401 gcgggctgcg atggtgaaat ttattattac cgcatcagac ttacagttac tgatgatgga
     2461 gggctcagcg gttacagtga aaaaagtgtt tatccaaatt gcacgactga ttgtaatggt
     2521 gtttttaatg gcactgcttt cattgattca tgcaacactt gtgttggtgg caacacagga
     2581 cttcaacctt gtacacaaga ttgtcatggt gactggggtg gtactgcttt cattgattca
     2641 tgcagcacat gcgttggtgg
//
LOCUS       DAOOOI010000186         2659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_6303_length_2659_cov_7.049923, whole genome shotgun sequence.
ACCESSION   DAOOOI010000186 DAOOOI010000000
VERSION     DAOOOI010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6303_length_2659_cov_7.049923"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1565)
                     /gene="purH"
                     /locus_tag="PKX59_11615"
     CDS             complement(36..1565)
                     /gene="purH"
                     /locus_tag="PKX59_11615"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOZ91545.1"
                     /translation="MKIKSALISVYNKNGFEPLIKALHQQQVTLFATGGTFDFIQSLK
                     IPITAVEDVTDYPSIFGGRVKTLHPKIFGGILNRRNDANDVMEVEKYNIPCIDLVVVD
                     LYPFEATVASGADESAIIEKIDIGGVSLIRAAAKNFNDVSIIASADDVTAFLQTYNQG
                     NGEVNLSFRKKLAQKAFAITSHYDSAIFNYFYSKNEQSEPVLNLQCNNSVTLRYGENP
                     HQRGYYFGNLNETFVQLGGKELSYNNMLDCDAAINLIAEFSNEKGVTAAIIKHNNACG
                     IATREKATDAWQAALAGDPVSAFGGIIVFNQHIDESLAKEINAIFFEVIIAPSFAEEA
                     VKVFSEKKNRIILQLKKQLVAKQSIKSALGGYLMQETDSEALSTDGWNNITLRKPTTT
                     ETEDMLFAVRVIRNLKSNAITLVKNKQLIGSGAGQTSRVDALKQAIAKAVNFGFNLKN
                     SVMASDAFFPFPDCVEIAKTAGIEAVVQPGGSIKDKDSIEYCNKNNMTMVLTGKRHFK
                     H"
     gene            1685..>2659
                     /locus_tag="PKX59_11620"
     CDS             1685..>2659
                     /locus_tag="PKX59_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ91546.1"
                     /translation="MNRIRIFLKLSAESIAFALHALRANMLRTVLSLLGISIGIFAII
                     SVFTVVDAMEKNVRTSVESLGDKTIFCQKWPWAFGGDYPWWKYWQRPLPSVKEMHQLA
                     ARSQSIEAFTFEAYLNGKTVKYLNNSAENVTVALVSHDFDKVKSFELSAGRYLTEMES
                     NSGRAVGIIGSNVANGLFGSASNALGKVFEVMGRKVEVIGVFKEEGKSLLETEMDNTV
                     VLPLNFGRNFVNIRSDRIDPFIKAKVKEGVPIQFANDELKSIMRSIRRLRPIEDDNFA
                     LNQSSLLSNNITNIFSALNMAGAIIGGFSILVGGFGIANIMFVSVKERT"
BASE COUNT          785 a          485 c          510 g          879 t
ORIGIN      
        1 tgcagacatt tttaacactt tgtctgccaa cagacttaat gtttgaaatg ccttttacct
       61 gttaatacca tagtcatgtt atttttgtta caatattcaa tagaatcttt atctttaata
      121 cttccacccg gctgtacaac agcttcaata ccagcagttt tggctatctc tacacaatcc
      181 ggaaacggaa agaatgcatc acttgccata acactgtttt tcaggttaaa tccaaaattt
      241 actgcctttg caatggcttg cttcaatgca tcaacacgag aggtttgccc ggcaccactt
      301 ccaatcaact gcttattctt aaccaaggta atagcattgc tttttaaatt acggataact
      361 ctcacagcaa aaagcatatc ctctgtctct gtagttgttg gctttcgcaa agtaatgtta
      421 ttccatccat ctgtgctcaa tgcttcactg tctgtttcct gcattaaata tccgcctaaa
      481 gcagacttaa tactttgctt ggcaacaagc tgttttttta attgaagtat aattctgttt
      541 ttcttttctg aaaaaacttt tactgcttct tctgcaaacg atggtgcaat tataacttca
      601 aagaaaatag cattgatttc tttagcgagt gattcatcta tatgctgatt aaaaacaatt
      661 attccaccaa atgcagaaac aggatcacct gctaatgctg cctgccatgc atctgttgct
      721 ttttctcttg tagcaattcc gcaggcattg ttgtgtttga taattgctgc agtcacacct
      781 ttttcattgc tgaattctgc aataagatta attgctgcat cgcaatcaag catgttgtta
      841 taggaaagtt ccttgcctcc taattgtaca aatgtttcat tcaggttacc gaaataataa
      901 ccacgctgat gtggattttc gccataacgc aaggtaactg aattgttgca ttgtaaattc
      961 aaaaccggct ctgattgttc attcttacta tagaaataat taaaaatggc agagtcgtaa
     1021 tgactggtta tggcaaatgc tttttgtgca agtttttttc tgaatgataa attaacttca
     1081 ccattgccct gattgtaagt ttgcagaaat gctgttacat catctgcaga ggcaatgata
     1141 gatacatcat taaaattttt tgctgcggca cgaattagtg atacaccacc aatgtcaatt
     1201 ttctcaatga tggcactttc atcagcaccg gatgcaactg tggcttcaaa aggatataaa
     1261 tcaactacaa ccaaatctat gcatgggatg ttgtattttt caacttccat cacatcattt
     1321 gcatcgtttc tgcgatttaa tattccacca aaaattttag ggtgtaatgt ttttactctt
     1381 ccaccgaata tggatggata atctgtcaca tcttcgacag cagtaatagg aattttcaat
     1441 gattgtataa aatcaaaagt tcctccagtt gcaaacaaag ttacctgttg ttgatgcaga
     1501 gccttgataa gtggttcaaa gccattttta ttatagacag aaataagcgc acttttaatc
     1561 ttcacttccg ggaattcttt aaattgatgg agagtttgca aaaataggta agtgaatcaa
     1621 ttaaaatttg ttaaactgaa aataaattca gagcaaatca tggcaacttt atacttttgt
     1681 tgcaatgaac cgaataagga tttttctgaa attgtcagct gaaagcatag catttgcact
     1741 gcatgctttg agggcaaata tgctcagaac agtgttatct ctattgggaa tctccatagg
     1801 tatttttgcc attatcagtg tttttacggt ggttgatgcc atggaaaaaa acgtacgcac
     1861 cagtgtagag tcgttgggag acaagactat tttttgtcaa aaatggccgt gggcgtttgg
     1921 cggcgattac ccttggtgga aatattggca gcgtccgtta ccttctgtaa aagaaatgca
     1981 tcagttggct gctcgctctc agagtattga ggcttttact ttcgaagcat atttgaatgg
     2041 taaaacagtt aagtacctga acaatagtgc agaaaatgta actgtagccc tagtgtcgca
     2101 tgattttgac aaggtaaaat ctttcgaact ttcagccggt cgttatctta ctgagatgga
     2161 gtctaactcc ggtagggctg ttggcattat aggcagtaat gttgctaacg ggctttttgg
     2221 aagtgcaagt aatgctttag ggaaagtttt tgaagttatg ggtaggaaag ttgaagtgat
     2281 tggtgttttc aaagaagaag gtaaaagtct gcttgaaaca gagatggata atacggtggt
     2341 tctgccatta aatttcggac gtaatttcgt taatattcgc agcgacagaa tagatccttt
     2401 catcaaagca aaggtcaaag aaggtgttcc cattcagttt gctaatgatg aattaaaatc
     2461 cattatgcga agcataagaa gactacgccc tattgaggac gataattttg cactcaatca
     2521 gtcaagtttg ttgagcaata acatcaccaa tattttcagc gcgctgaata tggcaggagc
     2581 tattatcgga ggcttttcta ttttggtggg tggctttggt attgcaaaca ttatgtttgt
     2641 ttcagtgaag gaacgtacc
//
LOCUS       DAOOOI010000187         2647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_6336_length_2647_cov_11.540123, whole genome shotgun sequence.
ACCESSION   DAOOOI010000187 DAOOOI010000000
VERSION     DAOOOI010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6336_length_2647_cov_11.540123"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..302
                     /locus_tag="PKX59_11625"
     CDS             141..302
                     /locus_tag="PKX59_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91547.1"
                     /translation="MFKIINKILIVLLLPMATNAQSFKASIIGGINASQVSGDELGGF
                     NKARDILRQ"
     gene            complement(271..549)
                     /locus_tag="PKX59_11630"
     CDS             complement(271..549)
                     /locus_tag="PKX59_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ91548.1"
                     /translation="MISINNLKISFDKHQVLDIEKYNFNTGKVHGIIGLNGAGKTTFF
                     NAVCKFLKRDEQAVLLNNKPFERHDAAYLETTNYFYDNITAEEYLSLY"
     gene            complement(533..961)
                     /locus_tag="PKX59_11635"
     CDS             complement(533..961)
                     /locus_tag="PKX59_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91549.1"
                     /translation="MIFLWLTTITIAGFYQFGEPLSLLYLHENAKQLLRIKIKNVLLL
                     YVKITLIPVLVNIFLHPDIWWVDLMIYILCMLVIICAVLIKYAVYRPNERLTAGSMLL
                     SIVSISPALPFTLPLPIIVAAIKYKSAIQNLENYFHDQHK"
     gene            1025..1096
                     /locus_tag="PKX59_11640"
     CDS             1025..1096
                     /locus_tag="PKX59_11640"
                     /inference="COORDINATES: protein motif:HMM:NF022969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HOZ91550.1"
                     /translation="MIASANVVALEFQTVAAANNYWK"
     gene            1182..1568
                     /locus_tag="PKX59_11645"
     CDS             1182..1568
                     /locus_tag="PKX59_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HOZ91551.1"
                     /translation="MRDIENINDIQLLVDSFYGKIRDDEKLGHIFNGIIKDRWPEHLE
                     KMYRFWQTILLEDQTYFGSPFVPHAKMPVDKTHFEQWIKLFSETVDENFVGEKAEQAK
                     WQGQRMAEMFHYKIEYIKNSSTTPLQ"
     gene            1565..2020
                     /locus_tag="PKX59_11650"
     CDS             1565..2020
                     /locus_tag="PKX59_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91552.1"
                     /translation="MTVVHLPLALIASTLWIGFVSAISFMEAWLKFKAPGVTIPLGLG
                     IGRLVFNALNKIEWVLATLIICNLILAKEQFINPIKSGHGVFYLIPVFILLFQTVWLL
                     PQLDARAQMHLNRQTVPPSKMHFVYVGLEIVKVVFLATFILNLFKNITT"
     gene            2017..>2647
                     /locus_tag="PKX59_11655"
     CDS             2017..>2647
                     /locus_tag="PKX59_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HOZ91553.1"
                     /translation="MNNEKLISDIDNKYQQTGENSETYLKGLLHAKPITYWDYVEVDT
                     LLSLQRPRTNFKDEEIFVMYHQVTELVLKMMVHEIKQMVFDPFAEHIWIDKINRLNRY
                     TSMLITSFDVMKYGMNYDDYNTFRSTLAPASGFQSAQFRFIEIYLTRLENLMNEEGRK
                     RLGNNPSIEDYFEHIYWKDAGMDRKTGKKSMTLKLFEEKYLESFIALAKK"
BASE COUNT          958 a          411 c          450 g          828 t
ORIGIN      
        1 cgactgagta aaaacaaaaa gcaatagaac tacaaggcgc atatttactg tttatttaat
       61 gcaagattaa cacttgaact gtaaataacc aacctttaca acctgttaat ttttactttt
      121 gttgtacata acatcgcact atgtttaaaa ttattaataa aattctgatt gtgcttttat
      181 tgccaatggc tacaaatgcg caatcattta aagcttccat cattggtggc atcaatgcat
      241 cacaagtttc cggtgatgag ttgggaggtt ttaataaagc gagagatatt cttcggcagt
      301 aatgttatca taaaagtaat ttgttgtttc cagataggcc gcatcatgac gctcaaaagg
      361 tttattattt aacaaaacgg cttgctcatc acgctttaaa aatttacata cagcattaaa
      421 aaatgttgtt tttccggcac cattaagacc tataatgcca tgtacttttc ctgtattaaa
      481 attatatttt tcaatatcaa gaacctgatg tttatcaaag gaaattttca gattatttat
      541 gctgatcatg aaagtaattt tctaagtttt gaatagcgct tttatacttt attgcagcaa
      601 caataatagg taatggaagt gtgaatggaa gtgccggtga aattgaaaca attgacagta
      661 acatgcttcc tgcagttaat ctttcattgg gcctgtaaac agcatattta atcagtacag
      721 cacaaataat aacaagcata caaagtatat aaatcattaa gtcaacccac caaatatccg
      781 gatggagaaa tatattcact aaaacaggaa tgagagttat ctttacataa agcaacaaaa
      841 cattttttat tttaattctt aaaagctgtt tggcgttttc atgtaaatag agtagtgaaa
      901 gtggttcacc aaattgataa aacccggcaa ttgttattgt tgtcagccaa agaaatatca
      961 atggaataaa ttttaagaaa caggtcgcat acagtacaat taaaaagact gcgacacaaa
     1021 acaactgata gcttctgcca atgtagtagc attagaattt caaactgtgg cggcagcaaa
     1081 taattattgg aagtaatttt ttatagttta ctgatgagaa aaacaattac agagtttgca
     1141 ttaaaattaa aattaaaaca aactaaactt ctgaagtggt gatgagagat attgaaaaca
     1201 taaacgacat acaattattg gtggatagct tctatggtaa aatccgtgat gacgaaaaat
     1261 tgggtcatat ctttaatggt attattaaag acaggtggcc cgaacatctt gaaaagatgt
     1321 atcgtttctg gcaaacaata ttgttagaag atcaaacata ttttggtagc ccttttgttc
     1381 cacatgctaa aatgccggtt gacaaaacgc atttcgagca atggataaaa ctcttctccg
     1441 aaactgttga tgaaaacttt gttggcgaaa aagctgagca agcaaaatgg caaggccaac
     1501 gtatggctga aatgtttcac tacaagattg aatatattaa aaatagttcc actactccac
     1561 tacaatgaca gttgtacact taccattagc tttaattgca tccacattat ggattggttt
     1621 tgtcagtgca atcagtttta tggaagcctg gcttaaattt aaagcaccgg gagttacaat
     1681 acctttaggt ctaggaattg gcagattggt atttaatgca ctcaacaaaa ttgaatgggt
     1741 acttgcaaca ttaatcatct gtaatcttat tttagcaaaa gaacaattta tcaatcccat
     1801 caaaagtgga catggtgtat tttatcttat ccctgtattt attttactat ttcagacagt
     1861 ttggttatta ccacaacttg atgcacgcgc acagatgcat ttaaacagac aaacggtgcc
     1921 cccttcaaaa atgcattttg tttatgtagg tcttgaaatt gtgaaagtgg tatttttagc
     1981 aacatttatt ttaaatctct ttaaaaacat aacaacatga acaatgaaaa actaatcagc
     2041 gatattgata ataaatatca gcaaacgggt gaaaattcag aaacatatct gaaaggactt
     2101 ttacatgcaa aacccattac ttattgggac tatgtggaag ttgataccct tttatcacta
     2161 caaagaccaa gaacaaattt taaagacgaa gaaatttttg tgatgtatca tcaggttact
     2221 gagctggtat taaaaatgat ggtgcatgaa attaaacaaa tggtgtttga tccatttgca
     2281 gaacatattt ggattgataa aattaatcgc ctcaaccgat acacatctat gctcattacc
     2341 tcttttgatg ttatgaaata tggaatgaac tatgatgact ataacacatt cagaagtaca
     2401 cttgctcctg ctagtggatt tcaatcggca caattccgtt ttattgaaat ttatctgaca
     2461 cgattggaaa atttaatgaa tgaagaaggt agaaaaagat tagggaacaa tccttcaata
     2521 gaagattatt ttgaacatat ctactggaaa gatgcaggca tggatcgtaa aacaggaaaa
     2581 aaatcaatga cattaaaact gtttgaagaa aaatatttgg aaagttttat tgcacttgca
     2641 aaaaaag
//
LOCUS       DAOOOI010000188         2640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_6360_length_2640_cov_9.658414, whole genome shotgun sequence.
ACCESSION   DAOOOI010000188 DAOOOI010000000
VERSION     DAOOOI010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6360_length_2640_cov_9.658414"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="PKX59_11660"
     CDS             complement(<1..937)
                     /locus_tag="PKX59_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ91554.1"
                     /translation="MFIQQLYTSCLAEAAYYIESDGQAAIIDPIRETTPYLSLSEQRH
                     ARITHIFETHFHADFISGHIDLANKTGAKIVYGPDAHTNYTVHNAKDGEEFTIGKLTI
                     KAIHTPGHTLESTCYLLLDENKNPHCIFTGDTLFVGDVGRPDLLDGSGISKEFLADKM
                     FDSLNSKIKTLPDDVIVYPAHGPGSACGKNIGKETWSTIGQQKQSNYYLKEKDKASFI
                     KNLTTGILPPPMYFFEDARINKQGYRPIEEVIVRNAKPLALEDFKNEVNKGTLILDTR
                     NADDFEKGFVPGSVNIGLNGQYANWVGTLIPIQQPI"
     gene            complement(1001..1888)
                     /locus_tag="PKX59_11665"
     CDS             complement(1001..1888)
                     /locus_tag="PKX59_11665"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOZ91555.1"
                     /translation="MNRFSIKDIEILTGIKPHTIRIWEQRYGIIKPQRTATNIRYYTE
                     EDLKLMLNVSLLNKMGFKISKITCLKPNEIEHKVLSLVEQANDADINLSALLNCMVRL
                     DEDTFEHLLNKYLETNGLESTMTRILFPFLYRIGLLWQTGSITPAYEHFFTNLVRQKI
                     IAAIDKQVLSPLPDAKRFLLFLPPNEPHELGLLYAHYVIRQRGHRSVYMGLNLPIDHI
                     ICAHECCQPDFIVTTLTSCIKINAIEEFIVQISKTFSKSKILLSGAQAVNRSLHLPQN
                     VQVISDFDDFIQYIENRVN"
     gene            complement(1961..>2640)
                     /locus_tag="PKX59_11670"
     CDS             complement(1961..>2640)
                     /locus_tag="PKX59_11670"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOZ91556.1"
                     /translation="PHIICGGFSREETENALIDLNFLGIDNVLLLRGDSEKHESHFTP
                     EPGGHAYALELVEQVVNMNVGTFLDDDLKGVEPTKFCIGVAGYPEKHFEAPNLNSDIS
                     FLKKKVDAGAQYIVTQMFYDNQKYFNFVDKCRKAGINVPIIPGLKPISNKKQTAILPK
                     TFFIDIPDDLIQEIEKCKSDDAVKQAGIDWAVTQAKELIKFGVPCLHFYTMGNPENIR
                     KIASQLF"
BASE COUNT          791 a          486 c          462 g          901 t
ORIGIN      
        1 ctattggttg ttgaattgga attagtgttc ccacccaatt agcatactgt ccattgagtc
       61 cgatatttac agagccggga acaaaacctt tttcaaaatc atcagcattt cttgtatcga
      121 gaatcagtgt tcctttattt acttcattct taaaatcttc taatgccaac ggctttgcat
      181 ttctgacaat tacttcttca atgggcctgt atccttgttt gttgattctt gcatcttcaa
      241 aaaaatacat gggtggtgga agaattcctg ttgtcagatt ttttataaaa gatgctttgt
      301 ctttttcttt caaataatag tttgattgtt tttgctgacc aatagtactc catgtttctt
      361 ttcctatatt ttttccacat gcagagcccg ggccatgagc cggataaaca ataacatcat
      421 caggcaatgt ttttattttt gaatttaaag agtcaaacat tttatctgct aaaaactctt
      481 ttgaaatacc actgccatca agcaggtcgg gtctgcctac atcaccaaca aacaaagtgt
      541 cacctgtaaa aatacaatga ggatttttgt tttcatcaag caataaatag caggttgact
      601 ctaaggtatg tccgggagta tgaattgctt taatagtaag cttaccaata gtaaactctt
      661 ctccatcctt tgcattatgt actgtatagt tggtatgcgc atcggggcca taaacaattt
      721 ttgcgccagt tttatttgct aaatcaatgt gtccactgat aaagtcggca tgaaaatgtg
      781 tttcaaaaat atgtgttatg cgtgcatggc gttgttccga caatgacaaa taaggtgttg
      841 tttcacgaat gggatctata attgcagcct gcccatcaga ctctatataa taggccgctt
      901 cggctaggca acttgtatat aattgttgaa taaacatatt tctatcagga tttaaaacaa
      961 agctacaatt tttacagtaa tgaataaaat aaagtatgca ttaattgact ctgttttcaa
     1021 tgtattgaat aaagtcatca aagtcagaga ttacctgaac attttgtggc agatgcaaac
     1081 ttcggtttac tgcctgtgca ccggaaagta atattttact ttttgaaaat gtttttgaaa
     1141 tctgtacaat aaattcttca atagcgttaa tctttatgca tgatgttaat gtagtcacaa
     1201 taaaatcggg ctgacaacat tcgtgagcac aaatgatatg atctatcggt aagttaagac
     1261 ccatataaac tgatctgtga ccacgttgtc tgattacata gtgtgcatac aacaacccta
     1321 attcatgcgg ttcgttaggt ggcagaaaga gtaaaaatct ttttgcatca ggcagtggcg
     1381 acaatacctg cttgtctatt gctgcaatta ttttctgacg tacaagattg gtaaaaaaat
     1441 gttcgtaggc cggtgtaatg gagcctgtct gccaaagcaa tcctattctg tataaaaagg
     1501 ggaacagaat tcttgtcatt gtactttcca acccatttgt ctccagatat ttatttagca
     1561 ggtgttcaaa agtatcctca tcaagcctga ccatacaatt caacaatgca ctgaggttta
     1621 tatcagcatc attggcctgt tctactaacg acaaaacttt atgttcgatt tcattcggtt
     1681 taagacaagt tattttcgaa atcttaaatc ccatcttatt caacaaagaa acattgagca
     1741 tcaacttcaa atcttcctct gtgtaataac ggatattggt tgcagttcgc tgtggtttaa
     1801 taataccata acgctgctcc catattcgaa tggtatgcgg cttaattcct gtaagaattt
     1861 ctatgtcttt aatcgaaaac cggttcaact gagtggagtt tgtgatataa acacctgtcg
     1921 tgtttaaaag tttcagcctt atgtatggat gcttgaaaca ttaaaacaat tgtgatgcaa
     1981 tttttctgat attctccgga ttgcccatag tataaaaatg aagacagggc acaccaaatt
     2041 taataagttc tttagcttgt gtcaccgccc aatcaatacc tgcttgtttt actgcatcat
     2101 cactcttgca tttttcaatt tcctgaatca ggtcatcagg tatatcaata aaaaaagttt
     2161 ttggtagtat ggcagtttgt tttttatttg aaattggttt tagtccggga attattggca
     2221 cattaattcc tgctttacgg catttatcta caaagttgaa atatttctga ttgtcgtaaa
     2281 acatttgtgt aacgatatac tgtgcaccgg catctacttt tttcttaagg aaagaaatgt
     2341 ctgagttcag attaggggct tcaaaatgtt tttcgggata accggctaca ccaatacaaa
     2401 attttgttgg ctctacacct ttaagatcat cgtctaaaaa tgtacccaca ttcatattta
     2461 ctacctgttc aaccaattca agagcatagg catgtccacc gggttcaggt gtaaaatgcg
     2521 attcatgttt ttctgaatca cctcttagta aaagcacatt gtctattcca agaaagttaa
     2581 ggtcaattaa tgcattctct gtttcttcgc gtgaaaatcc tccgcagata atatgaggaa
//
LOCUS       DAOOOI010000189         2640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_6361_length_2640_cov_7.513733, whole genome shotgun sequence.
ACCESSION   DAOOOI010000189 DAOOOI010000000
VERSION     DAOOOI010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6361_length_2640_cov_7.513733"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PKX59_11675"
     CDS             <1..430
                     /locus_tag="PKX59_11675"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOZ91557.1"
                     /translation="AVQLATYFGEYMPATQQFINFKLTLGAAALLGHIFPVFAGFRGG
                     KGVATLLGILWGVNPPAAVICMGVFLLFFLTTGYVSLGSMAAAVSFPFIVMWWLNETV
                     TSMNVFAIAVPILVLVTHQKNIERLLKKTESRVYLFRRKQ"
     gene            complement(437..1642)
                     /gene="pcaF"
                     /locus_tag="PKX59_11680"
     CDS             complement(437..1642)
                     /gene="pcaF"
                     /locus_tag="PKX59_11680"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HOZ91558.1"
                     /translation="MKQAYIVDGIRTPVGNYGGILSSVRPDDMAAHVIKDLMARNTGL
                     APEKIEDVILGCANQAGEDNRDVARMALLLAGLPVTVPGETINRLCASGMSAVINAAR
                     AIRTGDTSCMIAGGVESMSRAPYVMSKSSSAFARDVQLFDTSIGWRFVNSRMKEMYGT
                     DSMGETAENVATQYKINREDQDLFALNAQMKAAKSQRDGRFECEISPVIIPRKKGEPI
                     VASNDEFIKPDTSIEILSKLKPAFKKDGSVTAGNASGVNDGACALLLTDEETLNQNKL
                     KPMARIVSSAVAGVEPRIMGIGPVPAANAALKKAGLSWNNIDIIELNEAFAAQALACI
                     RQWNLDDRDERINPNGGAIAIGHPLGMSGARIILTAAHQLALTNKQYALCTMCIGVGQ
                     GYAVIIERC"
     gene            complement(1647..2030)
                     /locus_tag="PKX59_11685"
     CDS             complement(1647..2030)
                     /locus_tag="PKX59_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HOZ91559.1"
                     /translation="MMEHDRFSQWLGIKCLVMEPGKSVLQMQIREEMLNGFGLIHGGI
                     AFSLADSALAFASNAYGRLSVALECSISYPAPVHNGDIITATAEELSLTNRIGVYQIT
                     ITNQNNIKVAFFKGTVYRTSKEYFK"
     assembly_gap    2033..2062
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2155..>2640)
                     /locus_tag="PKX59_11690"
     CDS             complement(2155..>2640)
                     /locus_tag="PKX59_11690"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOZ91560.1"
                     /translation="VFSQGGTYNLKQNTIANYWRYSQRSTPAFFMNDFVNDGSSNVHI
                     PLNINVDNCIIYGNNDNEIEKDLLDSLADTYSFKNNLIKVDEKTTPISVIPAFINTLK
                     NQDPLFEDQSKYNFNLKDNSPAIDAGDGSLLTFPELNTDLNGGTRPQGAAPDLGAIEK
                     K"
BASE COUNT          741 a          520 c          510 g          839 t
ORIGIN      
        1 ggcagtacaa ttagctactt attttggaga atatatgccg gcaacgcaac agtttataaa
       61 ctttaaactt actttgggtg ctgcagcatt attaggacat attttccctg tatttgcagg
      121 attcagagga ggtaaaggtg ttgccacttt attaggtatc ctatggggtg tgaacccgcc
      181 agcagctgta atctgtatgg gcgttttttt acttttcttt ttaactacag gctatgtttc
      241 attaggctca atggctgctg ctgtttcttt tccatttatt gtaatgtggt ggctaaatga
      301 aacagtaacg agtatgaatg tttttgcaat agcagttcct atattggtgt tagttacgca
      361 tcagaaaaac attgaacgat tactcaaaaa aacagagagc agagtttatc ttttcagacg
      421 taaacaatag caacctttag catctttcaa taattactgc ataaccttgt ccaacaccaa
      481 tgcacatggt gcagagtgca tattgtttat tagttagtgc taactgatgt gctgctgtaa
      541 gaatgattct tgcacctgac attcccaatg ggtgaccaat tgcaatagca ccgccatttg
      601 gattgatgcg ttcatcacga tcatctaaat tccattgtct gatacatgct aatgcttgcg
      661 ctgcaaatgc ctcgttaagt tcaatgatgt caatattatt ccagctaagt cctgcttttt
      721 ttaatgctgc attagctgca ggaacaggcc caatgcccat aatgcgtggc tctacgcctg
      781 ccactgccga tgatactatg cgagccattg gcttaagttt attctgatta agggtttcct
      841 catcagttaa cagaagcgca catgcaccat cattcacacc tgaagcattt cctgccgtaa
      901 cactaccgtc ttttttgaat gcaggtttta gtttagataa aatctctatg cttgtatcgg
      961 gtttaataaa ttcatcatta gaagcaacta taggttcacc ttttttacga ggaatgatga
     1021 caggagaaat ttcacattca aacctgccgt cacgttgtga tttggctgct ttcatttgtg
     1081 catttaaggc aaaaagatcc tgatcttctc tgttgatttt atattgtgtt gcaacattct
     1141 ctgcagtttc acccatggag tcggtgccat acatttcttt catacgtgaa ttaacaaaac
     1201 gccagccaat gcttgtgtca aaaagttgga catcacgtgc aaaagccgaa gaagattttg
     1261 acataacata aggtgctcgg ctcatgcttt caacaccacc tgctatcatg catgatgtgt
     1321 caccggttct aattgctcgt gcagcattta ttactgccga cattcctgaa gcacaaagac
     1381 gattaatggt ttcaccggga acagtgacag gtagccctgc aagcaatagc gccattcttg
     1441 ctacatcacg attgtcttca ccggcctgat tggcacaacc taaaattaca tcttcaattt
     1501 tttcaggtgc aagcccggta tttcttgcca tcaggtcttt aatgacatgt gcagccatat
     1561 catcaggacg tactgaagac agtatgcctc cataatttcc tacaggcgta cgtatgccat
     1621 caactatata tgcttgtttc attctgttat ttaaaatatt ctttactagt tctgtaaaca
     1681 gttcctttaa aaaaagcaac cttaatatta ttttggtttg taatggtaat ctgataaaca
     1741 ccgattctgt ttgtcagtga aagttcttct gcagttgcag taataatgtc gccattatgt
     1801 acaggtgcag gataggatat tgaacattcc aaagcaacag acaatcttcc ataggcattg
     1861 gatgcaaaag caagcgcact gtcggcaagc gaaaatgcaa tgcctccatg aatgagtccg
     1921 aaaccattca gcatttcttc gcgaatctgc atctgaagaa cactttttcc gggctccata
     1981 actaagcatt ttattcccaa ccactgactg aaacggtcat gttccatcat gtnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nntttgggca agtgtatttt gatccatgac tgcaaatgta
     2101 atgattggta agatggtaga aattacagaa ataaaatgtg tagtaaaaaa agaattattt
     2161 tttctctatt gcacctaagt ccggtgctgc accctgtgga cgtgtgccac cattcaggtc
     2221 ggtgttgagt tccggaaatg tcagcaagga gccatcgcct gcatcaatag caggggagtt
     2281 atcctttaaa ttaaaattat atttcgactg atcttcaaat aaagggtctt gatttttaag
     2341 tgtattgata aaagcaggaa tcacacttat gggtgtggtt ttttcatcaa cttttatgag
     2401 attattttta aaagagtacg tgtctgcaag actatcaagt aaatcttttt caatttcatt
     2461 gtcgttgttg ccatagataa tgcaattgtc aacattgatg ttaagtggaa tgtgcacatt
     2521 gctactaccg tcattgacaa aatcattcat aaaaaatgca ggggtactac gttgcgaata
     2581 acgccaatag tttgcaatgg tgttttgttt aaggttgtaa gtgccaccct gtgagaatac
//
LOCUS       DAOOOI010000190         2637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_6372_length_2637_cov_16.953137, whole genome shotgun sequence.
ACCESSION   DAOOOI010000190 DAOOOI010000000
VERSION     DAOOOI010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6372_length_2637_cov_16.953137"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..797
                     /locus_tag="PKX59_11695"
     CDS             27..797
                     /locus_tag="PKX59_11695"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOZ91561.1"
                     /translation="MNSKEEIVKNWLPRYTGTAIEDFGKYILLTNFNNYVEIFAEQHQ
                     VEIMGTHRSMPNATANGITIINFGMGSANAATIMDLLTAIDPAACLFLGKCGGLKRKN
                     ELGDLILPIAAIRGEGTSNDYMPAEVPALPAFSLQKAVSTTIRDYDKDYWTGTVYTTN
                     RRVWEHDEEFKQYLRKMRCMAIDMETATIFTTGFVNKIPSGALLLVSDQPMIPDGVKT
                     EKSDLNITRKFVKDHINIGIDSLNQLKNNGLTVKHLRF"
     gene            804..1868
                     /gene="holA"
                     /locus_tag="PKX59_11700"
     CDS             804..1868
                     /gene="holA"
                     /locus_tag="PKX59_11700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOZ91562.1"
                     /translation="MPERKFEEILKDLKNKVYHPLYFLQGEEPYYIDAISDYVEKNVL
                     NDMEKEFNQTILYGREADAMAIVSAARRFPMMSNYQVVIIKEAQDVKWFGSKEKEEDK
                     TKDKSKEKEDPMMLYLQNPTTTTILLFCYKYKTIDSRTKKGKLIKDKAQFLETKKLYD
                     NQLPAWIKNYLKDRKIKADDHAIEMLAEYLGNDLSKINNEVGKMLINLKDKSVITVND
                     IREHIGVSKDYNVFELQNALAERNILKANRIVNYFEANPKNNPIQLIIGSLFAYFNKI
                     LIYHFTADKSSASLASAIGISPFFVKDYERAAKNYPVRKLEQIIGWLREYDLKSKGVG
                     STDQVPTGELLREMVFKIIH"
     gene            1872..2609
                     /locus_tag="PKX59_11705"
     CDS             1872..2609
                     /locus_tag="PKX59_11705"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HOZ91563.1"
                     /translation="MIRVSVVSYLNSKPFVAGLTQNSIKNKIDLSLDIPSVCAEKLIN
                     GKVDIGLIPVAVLPLVKNAQIITSFCIASNGNVNSVLLLSQVPLNEIKQIWLDYHSRT
                     SVMLAQLLAKEFWKIDVSWLPSEKGFENKIEGQTAGVVIGDRALALKSNFKYVYDLAT
                     EWKKHTGLPFVFACWVSNKSLPEDFIVAFSHALNQGVAMIPELSKQLQPDYLFDVKSY
                     LTENIDFRLDEEKEQALKLFLERIELK"
BASE COUNT          934 a          412 c          514 g          777 t
ORIGIN      
        1 attaagctat caagaaataa aaatttatga attcgaaaga agaaattgta aaaaactggc
       61 tgccgcgata tacaggtact gctattgaag attttggtaa atacatatta ctcaccaact
      121 tcaataatta tgttgaaatt tttgcggaac aacatcaggt agaaatcatg ggcacacatc
      181 gttccatgcc taatgctacc gctaatggta ttacaataat taatttcggc atgggcagtg
      241 caaacgctgc aacaattatg gatttgctta cagctattga cccggctgca tgtttgtttc
      301 ttggaaaatg tggcggacta aaacgtaaaa atgaattggg cgatttaata cttcctattg
      361 ctgcaattcg tggagaagga acatctaatg actatatgcc cgcagaagtt cctgcattgc
      421 ctgcatttag tttgcagaag gccgtgtcaa caaccataag agattatgac aaggattact
      481 ggacagggac tgtttatacc accaaccgaa gagtttggga acatgatgag gagtttaaac
      541 aatacctgcg taaaatgcgt tgtatggcta ttgatatgga aacagctaca atttttacaa
      601 caggatttgt caataaaatt ccttcgggag cattattgtt agtaagcgat cagccaatga
      661 tacccgatgg tgttaaaaca gaaaaaagtg atttgaatat tacaagaaaa tttgtaaaag
      721 accatatcaa tattggtatt gattcactaa atcaattaaa aaataacggt cttactgtaa
      781 agcatctgcg tttttaatag actatgcctg aacgcaaatt tgaggagata ttaaaagatc
      841 ttaaaaacaa ggtttatcat ccgttgtatt ttctgcaggg cgaagagcct tattacattg
      901 atgccatcag cgattatgtt gaaaaaaatg tgctgaatga catggagaaa gaatttaatc
      961 aaaccattct ttacggtcgc gaagctgatg ctatggctat tgtgagtgct gcaagaagat
     1021 ttccaatgat gtcgaactat caggttgtaa ttattaaaga ggcacaggat gtaaaatggt
     1081 ttggcagcaa agagaaagaa gaggataaaa caaaagataa aagcaaggag aaagaagacc
     1141 ccatgatgct ttatcttcag aatcctacta ccacaaccat tctgttgttt tgctataaat
     1201 acaagaccat tgacagcaga acaaaaaaag gaaaactgat aaaggataaa gcacagtttc
     1261 ttgaaacaaa aaaattatat gacaatcagt tgcctgcatg gattaaaaat tatctgaagg
     1321 acagaaaaat taaagctgat gatcatgcta tagaaatgct tgcagaatat ttagggaatg
     1381 atttgtcaaa aatcaacaat gaagttggaa aaatgctgat taatctaaaa gacaaatcgg
     1441 ttattacggt aaacgatatt cgtgagcata ttggtgtcag caaagactat aatgtttttg
     1501 aattgcagaa tgcattggca gagcgcaata ttttaaaagc aaacagaatt gtaaattatt
     1561 ttgaagccaa tccaaaaaac aatcccattc aactgattat tggtagcttg tttgcctact
     1621 ttaataaaat tctgatttat catttcactg ccgacaaatc ttctgcatca cttgcttctg
     1681 caataggcat tagtccgttt tttgttaaag attatgaacg tgccgctaaa aattatcctg
     1741 tcagaaaatt agagcagatt attggctggt tgcgcgagta tgatttaaaa tcgaaaggtg
     1801 taggcagtac agatcaggtg ccaacaggag aactgctcag ggaaatggta tttaaaatta
     1861 ttcattaaaa agtgattcga gtttctgtag tttcttatct caactccaag ccatttgtcg
     1921 cagggttgac acaaaatagt attaaaaaca aaattgattt gtcattagac atcccatctg
     1981 tttgtgcaga aaaactaatc aatggcaaag tagatatcgg attgatacct gtggcggttc
     2041 tgccattagt aaaaaatgca caaatcatta cttctttttg tattgcctca aatggcaatg
     2101 tcaactctgt gttgttgcta agtcaggttc ctttaaatga aataaaacaa atctggcttg
     2161 attatcatag cagaacttcg gtaatgctgg cacagttact tgcaaaagag ttttggaaaa
     2221 ttgatgtcag ttggcttcct tccgaaaaag gatttgaaaa taaaatagaa ggtcaaactg
     2281 ccggagttgt tataggcgac agagcattgg cattgaagtc gaatttcaag tatgtgtatg
     2341 accttgccac agaatggaaa aaacatacag gcttaccttt tgtctttgct tgttgggtaa
     2401 gtaataaatc attacctgaa gattttatag ttgcattttc tcatgcactc aatcaaggag
     2461 tagcaatgat tcctgaatta agtaagcaat tacaacctga ttatcttttt gatgtaaaaa
     2521 gttacttaac tgaaaacatt gacttcagac tagatgaaga aaaagaacaa gcattgaaat
     2581 tgttcttgga acggattgag ttaaagtaaa tcaaaacgca attttaaaat gccttta
//
LOCUS       DAOOOI010000191         2631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_6389_length_2631_cov_11.605978, whole genome shotgun sequence.
ACCESSION   DAOOOI010000191 DAOOOI010000000
VERSION     DAOOOI010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6389_length_2631_cov_11.605978"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2180)
                     /locus_tag="PKX59_11710"
     CDS             complement(<1..2180)
                     /locus_tag="PKX59_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91564.1"
                     /translation="MINKSCLTVFLAAAITVGSASAKENTGQKPVHKGNVVKNASLRI
                     TAPCNEPTAQTDLQINNVRARVFSAGDMWWNLVDGANSKAEYEIPKGTGKTSLFAGSV
                     WIGGYDATGNLKVAAQTYRQSDANDYFTGPIGVQGGNMSTDVSICNQYDRIWSVTKQQ
                     VRDFVSTGVATRDIREWPGNGDQSRGELQLLAPFEDVNGDGLYISDSGDYPRYLLSGE
                     YPAPSDPNKLPCDDYIFGDQTLWWVFNDIGNIKKETNSAAIGLEFRAQAFAFSTDDEI
                     NNMTFYKYQIINRSNQAFDSTYFGWWVDPDLGDAFDDYVGCDVNLGLGFCYNGDPDDG
                     SGTIDGYGINPPAVGADFFQGPLADPNDGIDNDRDGVVDEPGEQIKMAYFIYYENDFN
                     GVGGNPQVSDDYYQFLSGSWKNGQRWTRDGAMGQTPPSPTVPVTNFMFPGTTDPAFPG
                     SDWSMASANITPFDRRFLQSAGKFTLQPGAVNYITTGMVWARTNNGGPLASVDLLKLS
                     DNKAQGLFDVCFKTLDGPDAPDVQITELNNKVILSLKNFNNDRIEKYSQLDPSIPTLV
                     QPDEEQRKFKFQGYLIFQLKNASINTSDIFDPSKTSSSSVMSGDNYRLISQVDIKDTV
                     TNLVNYFWAPEIPGYRPLTVAFNPDQLTGTNSGLKHSFVIDKDAFTNKPLVNHKAYYY
                     VVVSYAYNNFKPFEYIVPNSSNFTQQKPFKVGRNNIKVMSAIPHA"
BASE COUNT          726 a          576 c          458 g          871 t
ORIGIN      
        1 gcatgaggta tggccgacat tacctttatg ttgtttctgc ctaccttaaa tggtttctgc
       61 tgtgtgaagt tcgatgagtt tggtactatg tattcaaagg gtttgaagtt gttgtaggca
      121 tagcttacta ctacataata atatgcctta tgatttacca agggcttgtt ggtaaaggca
      181 tctttgtcta ttacaaagct atgtttcaat ccactgtttg ttcctgttaa ctgatcggga
      241 ttaaatgcca ctgtcaacgg acggtagccc ggtatctcag gtgcccagaa gtaattaact
      301 aagtttgtta ctgtatcttt tatgtcaacc tgacttatca ggcgatagtt gtcaccactc
      361 ataacacttg atgaggaggt cttggatgga tcaaatatat ctgaagtatt tatgctcgca
      421 ttctttaact ggaatatcag atatccctga aacttaaact tgcgttgctc ttcatcaggt
      481 tgtaccagtg ttgggataga tggatctaac tgtgaatatt tttctatcct atcattattg
      541 aaattcttta atgaaagaat aactttattg tttagctctg ttatctgtac atccggtgca
      601 tcagggccgt ccaatgtctt gaaacataca tcaaacaagc cttgtgcttt gttatcgcta
      661 agctttaata aatctactga tgctaatgga ccgccattgt tcgtacgtgc ccacaccata
      721 cctgtggtaa tatagtttac tgctcccggt tgcaaagtaa attttcccgc tgactgcaaa
      781 aaccttctgt caaatggtgt aatgtttgct gaagccatgc tccaatcact tcccggaaat
      841 gcagggtctg ttgtacctgg aaacatgaaa ttagtaactg gtacagttgg tgagggtggt
      901 gtctgaccca tagctccatc tcttgtccag cgctgaccgt ttttccatga cccggacaaa
      961 aattgataat aatcatccga cacctgcgga ttacctccta caccattgaa atcattttca
     1021 tagtaaataa aataagccat tttaatctgc tcaccgggct catcaactac gccatctctg
     1081 tcattatcta ttccatcatt agggtctgct aagggtcctt gaaaaaagtc tgcacccact
     1141 gcaggtggat ttattccata accatctatg gttccgcttc catcatcagg atccccatta
     1201 taacaaaaac ctaatcccaa atttacatca cagcctacat aatcatcaaa tgcatcaccc
     1261 aaatcagggt caacccacca gccaaagtat gtgctgtcga aggcctgatt gctgcggttg
     1321 atgatctgat acttataaaa tgtcatgttg ttaatctcat cgtccgtgct gaaggcaaat
     1381 gcctgtgctc tgaactcaag acctattgct gcactattag tttccttctt aatatttcct
     1441 atatcattaa atacccacca caaggtctgg tctccaaaaa tataatcatc acatggcagt
     1501 ttatttggat cgcttggtgc aggatattct ccactcaata aatatcgtgg ataatcgcca
     1561 ctatccgaaa tatacaagcc atctccgttt acatcttcaa atggtgccag cagttgcaac
     1621 tcgcctcttg actgatcgcc attgcccggc cactctctga tgtcgcgtgt agctactcct
     1681 gttgacacaa agtcgcgcac ctgttgcttg gtcactgacc atattctgtc gtactgatta
     1741 cagattgata catctgtact catgtttcca ccctgaacac caattggacc cgtaaagtaa
     1801 tcgttggcat cactttgtct gtatgtttgt gctgcaactt ttaagtttcc tgtggcatcg
     1861 tatccgccta tccataccga gccggcaaac aacgatgttt tgcctgtacc ttttggtatt
     1921 tcgtactctg ccttggagtt agctccatct acaagattcc accacatgtc tccggcagaa
     1981 aatactcttg cccgtacatt gttaatctgc aaatctgttt gtgccgtggg ctcattacag
     2041 ggggctgtaa ttctcaatga cgcgtttttt acaacattgc ctttatgtac cggcttctga
     2101 cctgtattct ccttggctga tgctgaacct actgtaattg ctgctgccag aaataccgtt
     2161 aaacaactct tattaatcat ttgtgctttg ttttagatat ggtttataat cacttttata
     2221 attcaaaata acacttgtta aatatgcctt tatactctaa gacaacatga ttaccaattt
     2281 cgttttctat tttgataaaa attattattt taattttttc caattattgt attgggtgat
     2341 ttttgcaatt ataatttcaa gcgttttcaa actattttat cttcgtttat ttgaggtatt
     2401 aaaaatatga aattaaataa taattacaaa tagtgaaaag caaacttttt ccggcaatta
     2461 attttaaact tcttcaacat tatatttgaa aaacagaatc aatggcaaaa aaaatggctg
     2521 ctatgaaaac acagcagcca ttcaaatatt ccaaattaaa cttactctcc gttagttata
     2581 taatcaagac gtggctcggt atcaccggat gcaccaatta caccacggaa a
//
LOCUS       DAOOOI010000192         2576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_6536_length_2576_cov_15.459342, whole genome shotgun sequence.
ACCESSION   DAOOOI010000192 DAOOOI010000000
VERSION     DAOOOI010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6536_length_2576_cov_15.459342"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PKX59_11715"
     CDS             <1..510
                     /locus_tag="PKX59_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOZ91565.1"
                     /translation="NGQYANWVGTLIPIQQPIVLVTENGKEEESVLRAARVGYENITG
                     YLEGGIESWKNNGLEFHTISSVDAATFTNLLKTGSYTALDVRKPGEYESSHVKDSVFV
                     SLSDIPQHYNKLDKSITYLIFCGGGYRSMIAASLLKKNGFENVVNVYGGFGKIKDCSI
                     SLETSQPVM"
     gene            507..806
                     /locus_tag="PKX59_11720"
     CDS             507..806
                     /locus_tag="PKX59_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOZ91566.1"
                     /translation="MNTVHEITTSNTNVLIDFYADWCEPCKWLNPVLEELTQQLPDLY
                     IHKINIDQFTEEAAAFEIKSVPVLILFVDGKQVWRMNGFKYAKELAKELKEFLKA"
     gene            903..>2576
                     /locus_tag="PKX59_11725"
     CDS             903..>2576
                     /locus_tag="PKX59_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOZ91567.1"
                     /translation="MSKKNIKFTKTQLEKLKQELLQVFLQHPRATFNPKQVWRKAEIN
                     LPEEYISVVLNADKHYIDETIQSLILELFQEGELLEVQQFRYKLLPAERFIEGNIQVT
                     SKGEGYVMNEGFEEDIQIPAHLMANALNGDKVRISLLAHREGRRQQGEVVEVLSRSRT
                     QFAGTIQHSGSYAFVVCDDPKMHVDIFIPGKQLNGARNGDKVIAEITLWSADASNPEG
                     KIVRVLGAPGEHTAEMNAIIVEYGLPEAFPDEVEKETLKISDVISKEEIKKRRDFRKV
                     TTFTIDPVDAKDFDDALSIQQLPNGNWEMGVHIADVSHFLKLGSAMDEEAFNRATSIY
                     LVDRVIPMLPEKLSNMVCSLRPNEEKLCYSAVFEMNEKAEVLNEWFGRTVIFSDHRFT
                     YEEAQQVIETNEGPFVKEIGVFHRLAQQLRKERHRKGAISFEKTEVKFRLDEKGNPIG
                     IFLKESKDSNKLIEEFMLLANRRVAEFIGKGVAKGKKDKTQKTFVYRIHDVPVEEKLS
                     DFKTFAAQFGYRISTASGKETAHSLNKMLKDVEGKKEQSMIEQLAIRSMA"
BASE COUNT          888 a          424 c          564 g          700 t
ORIGIN      
        1 aatggacagt atgctaattg ggtgggaaca ctaattccaa ttcaacaacc aatagtactg
       61 gtaacagaaa acggaaagga agaagaaagt gtacttcgtg ctgcgcgcgt gggttacgaa
      121 aacataacag gttatcttga aggaggaata gagagctgga aaaacaacgg actggagttt
      181 catactatca gctctgttga tgcagccaca tttaccaatt tactaaaaac aggatcatat
      241 actgctttgg atgtaagaaa gcccggtgag tatgaatcat cacatgtaaa agattctgtc
      301 tttgtttcgt tgagcgacat tccacaacac tacaataagt tggataaatc aataacgtat
      361 ctgatatttt gtggtggagg ctatcgcagt atgatagcag catcgttact aaaaaagaat
      421 ggatttgaaa atgttgttaa tgtgtatggc ggctttggaa aaattaaaga ttgcagcata
      481 tcattagaaa cttcacaacc tgtgatgtga acacagtcca tgaaataaca acatccaata
      541 ccaatgtgct gatagatttt tatgccgatt ggtgcgaacc ctgtaagtgg ctcaatccgg
      601 ttttggagga gttgacgcaa caacttcccg atttgtatat ccataaaatc aatattgacc
      661 aatttactga agaggctgca gcatttgaaa taaagagtgt gcctgtgctg atactttttg
      721 ttgatgggaa acaggtttgg cgtatgaacg ggtttaagta tgcgaaagaa ttagccaaag
      781 agttaaagga gttcttaaaa gcctgagacc ttactttata gctgttgaaa acatttattt
      841 tttaatgtgt agcatgacta acatctgttt cactacacgt aattttacaa tcaaacgaaa
      901 acatgtcaaa gaagaatata aaattcacaa aaacacaact tgaaaaatta aaacaagaat
      961 tgctacaggt ttttctgcaa cacccgcgtg ctacttttaa tcccaaacag gtttggcgca
     1021 aagcagaaat aaatttgccc gaagaatata tttcagtagt gctgaatgct gataaacatt
     1081 atatagacga aacaattcaa tctttaattt tagagctgtt tcaagaagga gagttgctgg
     1141 aggttcagca gtttcgttat aagttattac ctgcagaaag gtttattgaa ggaaacattc
     1201 aggtaacttc caaaggcgaa ggatatgtga tgaatgaagg gtttgaagag gatatacaaa
     1261 ttcctgcaca tttaatggca aacgcattga atggcgacaa agtcagaata agtttattag
     1321 cgcatcgcga aggcagaaga caacaaggag aagtggttga agttctcagt cgaagcagaa
     1381 cacaatttgc cggcacaata caacattcag gcagttatgc atttgtagtt tgcgatgatc
     1441 ctaaaatgca tgtagatatt tttattcccg gaaaacaact taatggtgcc cgcaatggcg
     1501 ataaagtaat tgcagagata accttatggt ctgccgatgc cagtaatccc gaaggaaaaa
     1561 tagtgcgtgt tttaggtgct cccggtgaac atactgcaga gatgaatgcc ataattgttg
     1621 agtatggtct tccggaagcc tttcctgatg aagttgaaaa agaaacatta aaaatttctg
     1681 atgtcatttc gaaagaagaa attaaaaaac gcagagactt cagaaaagta accaccttta
     1741 ctattgaccc ggttgatgca aaagattttg atgatgcatt aagcattcag cagttgccta
     1801 atggcaactg ggaaatgggt gtacacattg ccgatgtttc acatttctta aaactgggta
     1861 gtgccatgga tgaagaagcc ttcaacagag caacttcaat ttatttggtt gacagagtta
     1921 ttcctatgtt gccggaaaaa ctgtcgaata tggtttgctc tctaagacct aatgaggaaa
     1981 aactttgcta ctctgccgtt tttgagatga atgaaaaagc agaagtcctg aatgaatggt
     2041 tcggcagaac tgtaattttt tcagatcatc gctttactta tgaagaagca cagcaggtaa
     2101 tcgaaaccaa cgaaggccct tttgtaaaag agattggtgt gtttcaccgg ctggcacagc
     2161 aacttcgcaa agagcgtcac cgtaagggag caatcagttt tgaaaaaaca gaagtaaaat
     2221 tcaggcttga tgaaaaggga aatccaattg gaatatttct aaaggagagc aaagactcca
     2281 ataaactgat tgaagagttt atgttacttg ccaacagacg tgtggcagag tttattggca
     2341 aaggtgttgc aaaagggaag aaagataaaa cacaaaaaac ttttgtttat cgtatccacg
     2401 atgtgcctgt tgaagaaaaa cttagcgact ttaaaacttt tgcagcacag tttggctata
     2461 ggattagcac cgcatcagga aaagaaacag ctcattcact caataaaatg ctgaaagatg
     2521 tggaaggtaa aaaagagcag agtatgattg agcaattggc tatacgcagt atggct
//
LOCUS       DAOOOI010000193         2574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_6544_length_2574_cov_20.949980, whole genome shotgun sequence.
ACCESSION   DAOOOI010000193 DAOOOI010000000
VERSION     DAOOOI010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6544_length_2574_cov_20.949980"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..246)
                     /locus_tag="PKX59_11730"
     tRNA            complement(163..246)
                     /locus_tag="PKX59_11730"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(210..212),aa:Leu,seq:cag)
     gene            complement(274..355)
                     /locus_tag="PKX59_11735"
     tRNA            complement(274..355)
                     /locus_tag="PKX59_11735"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(319..321),aa:Leu,seq:gag)
     gene            479..1585
                     /locus_tag="PKX59_11740"
     CDS             479..1585
                     /locus_tag="PKX59_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOZ91568.1"
                     /translation="MFNKFHNSLNTLADPLWIAGPCSAEDEEHLTELAIRLKISGAHI
                     LRAGAWKPRTRPGAFEGFGAPALKWIRAAGDVSGLPVITEVASAKHVEQALAAGIDMF
                     WIGARTTVNPFLIQEIADALKGVDIPVFVKNPVNPDVKLWMGAIERFMQNGISRIASI
                     HRGFSSFENSTYRNKPNWELAIELRRLLPNVPIICDPSHISGDANMVPTLTQIAFDLQ
                     YDGVMVEVHLNPVKALSDAMQQITPEDFSRLRKQIIVRQPTAKDPVELSKLHDLRDLI
                     DELDSEIIEKIAGRMEVARKIGQYKDQNNMAILQLDRFKEILRTRTETGLRNQLTPDF
                     ILRLYSLIHEESIAQQDKQMTKPELRASSKAEKH"
     gene            complement(1594..1743)
                     /locus_tag="PKX59_11745"
     CDS             complement(1594..1743)
                     /locus_tag="PKX59_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91569.1"
                     /translation="MSATNANSAVEYSYHKAHIFFSFDYYNPVKNQSTPVFYLGSGRN
                     YYQQN"
     gene            1686..2573
                     /locus_tag="PKX59_11750"
     CDS             1686..2573
                     /locus_tag="PKX59_11750"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HOZ91570.1"
                     /translation="MYVLCDTNTQQHCLHLLQTLFPQTKSASLYCIQQGESFKTIETV
                     TGFWKFLLQQQADKHTVIINLGGGVITDLGGFAASLFKRGVDFIHVPTTLLAMVDAAI
                     GGKNGVNIDGYKNQSGSIVLPRYVYVDTDFLESLSQDEKVNGYAEMIKHALVADQEFY
                     KVLSEQCSFEKLQSIGKAIAIKLSIVAIDPFEKKERKLLNFGHTIAHAIESLYYSQQQ
                     DISHGKAVAAGIIVESYISNKMGFISDAELKQITDFIQSLFKFDEKDIVVEKLLPFMM
                     ADKKNISTNCRNIMSDAMI"
BASE COUNT          813 a          443 c          545 g          773 t
ORIGIN      
        1 ataatgtaat tctgtcatga attgtcattc gaaattacaa aatcagaaaa cgtttattta
       61 tatcttattc aaatttattt ttgtagggtt tattgtcctt tttagtaatt caggtttagt
      121 aaaaataagc tagtgcataa atagggtggc tctataaaaa tgtgcccgag aggagactcg
      181 aactcctaca ggttttaccc cacaggcttc tgagacctgc gtgtctacca atttcaccac
      241 ccgggcaaat attttttcag caattagttt aagtgcccgg gacgagattc gaactcgtac
      301 acctattggc gccaccccct caagatggtg cgtctaccaa tttcgccacc cgggcattat
      361 gggctgcaaa agtaataaat ttaacttctg taattttaat cccgctgaat attccaatac
      421 actattgcat agcaatgctg ctttctgcac ttgaaatgtg ttctttatct ttgcactaat
      481 gtttaataag tttcataact cactgaacac tcttgccgac ccattgtgga ttgcaggtcc
      541 atgcagtgcc gaagatgagg agcatctgac tgaattagct atcaggctaa agattagcgg
      601 tgcacatatt ttgcgtgccg gtgcttggaa acctcgcacc cgacccggtg cctttgaagg
      661 ctttggcgct cctgcattga aatggatcag agcagcagga gatgtttccg gcctaccggt
      721 tattaccgaa gtggcaagcg ccaaacatgt ggagcaggca cttgctgccg gcattgatat
      781 gttttggatt ggcgcacgta caactgtaaa tccattttta attcaggaaa ttgctgatgc
      841 tttaaaaggt gtagatattc ctgtgtttgt aaagaatccc gtcaaccctg atgtaaaatt
      901 gtggatgggt gccatcgaac gttttatgca aaatggaatt agtagaattg cttctattca
      961 tcgtggtttt tctagtttcg aaaacagtac atatagaaat aagcccaatt gggaattagc
     1021 cattgaactc agacgcttgt tgcccaacgt tcccattatt tgcgacccaa gtcatattag
     1081 cggtgatgct aatatggtgc caacactgac acaaattgca ttcgatttac agtatgatgg
     1141 tgtgatggta gaagtgcatt taaatcctgt aaaggcattg agtgatgcaa tgcagcaaat
     1201 cacacctgaa gattttagtc gtttaagaaa gcagattatt gtaagacaac ctacggcaaa
     1261 ggatcctgtt gaactaagta agctgcacga tttacgcgat ctgattgatg aattggatag
     1321 tgaaatcatt gaaaagatag cgggtagaat ggaagtggca cgaaaaatag ggcagtataa
     1381 ggatcagaat aatatggcta ttctgcagtt agatcgtttt aaggaaattc tcagaacaag
     1441 aactgaaaca gggttacgaa accaactcac accagatttt attttaagat tatatagcct
     1501 gatacatgaa gagtcaattg cgcaacagga taagcagatg acaaaaccgg aactcagagc
     1561 ctcatcaaaa gcagaaaaac attagagggg tgattaattt tgctgatagt agttacgtcc
     1621 tgacccaagg tagaaaacag gagttgattg attttttaca ggattgtaat aatcaaaaga
     1681 gaaaaatata tgtgctttgt gatacgaata ctcaacagca ctgtttgcat ttgttgcaga
     1741 cattgtttcc gcaaacaaaa tctgcttctt tatattgcat acaacaagga gaatctttta
     1801 aaacaattga aacggtaacg ggattctgga agtttctttt acaacaacag gctgataagc
     1861 atactgtgat tataaatctg ggtggaggtg taattacgga tttaggaggc tttgcggctt
     1921 ctctgtttaa aagaggagtt gattttattc atgttcctac aaccttgctg gcaatggttg
     1981 atgctgctat tggaggtaag aatggtgtaa atattgatgg ctataaaaat caaagtggga
     2041 gtattgtttt gccaagatat gtttatgtag atacagattt tttagagtcg ctttcacaag
     2101 atgaaaaagt caatggctat gcagaaatga taaaacatgc acttgttgcc gatcaagaat
     2161 tttataaagt actttcagag caatgcagtt ttgaaaagtt acaaagcatt ggaaaggcta
     2221 ttgcaataaa actaagtatt gttgcaatag atccatttga aaagaaggaa cgtaagttgc
     2281 tcaactttgg ccacactata gctcatgcaa tagagtcgtt atattattcg caacaacaag
     2341 atatttcaca tggtaaggca gttgctgccg ggataattgt tgaatcttac atctctaaca
     2401 aaatgggctt tatttcagat gctgagctaa agcaaatcac tgactttatt caatcactgt
     2461 ttaagtttga tgaaaaagat attgttgtag aaaaattgtt gccatttatg atggctgata
     2521 agaaaaacat ttcaaccaat tgcaggaata tcatgtctga tgcgatgata taaa
//
LOCUS       DAOOOI010000194         2537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_6638_length_2537_cov_9.096696, whole genome shotgun sequence.
ACCESSION   DAOOOI010000194 DAOOOI010000000
VERSION     DAOOOI010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6638_length_2537_cov_9.096696"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="PKX59_11755"
     CDS             <1..1325
                     /locus_tag="PKX59_11755"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOZ91571.1"
                     /translation="SITNKSGVAYNPDFNIQEAIRKIEGMQIYNGSMTYWPGSVIENW
                     WATAYALHFLTEARKAGYAVNENTINRTFEYLKSKVKTKETETVYFANANNVIEKQVR
                     VKREIIYSLYLMALNDRRDLSMMNYYKANHSQLTLDSKYMLALSYLAIGDTKSYLALL
                     PEKFAGEKSQRSLSGNFSSYIRDQALALNCLLETDPDNAQIPTMARTLSQLIRGEKWL
                     STQERAFALLALGKFAKRSTSANVRATVFADGKQIGTFNGNDLVINNNLAGKKIEIRT
                     EGKGQLYYFLEVSGIKESLRLKDEDSKLNVRRSYFNRFGQQINNNFKQGDLIVVQLAL
                     SSPTGIDVENVVLTDLIPSGFEIENPRIVDDREMTWIKSKSQPDYFDIRDDRINLYTQ
                     AGKTIKYFYYTVRAVNTGSFVVGPVSADAMYDGEFHSYSGGGRVKVVK"
     gene            1322..1765
                     /locus_tag="PKX59_11760"
     CDS             1322..1765
                     /locus_tag="PKX59_11760"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOZ91572.1"
                     /translation="MKSAIKGFRSAVLKSAIVFAVCMLLVYLLAWLIPFNVNIPYAVT
                     VTDSRGEVLHAFLSKDEKWRMKANLNEINPQLVKAIIQKEDRWFYFHYGVNPVAVGRA
                     VVNNLIYQRKTSGASTITMQVSRLLWERLLFVVQNFACKLLYSVH"
     gene            complement(1830..>2537)
                     /locus_tag="PKX59_11765"
     CDS             complement(1830..>2537)
                     /locus_tag="PKX59_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HOZ91573.1"
                     /translation="DQVINAAAKMKSMSGGQYGVPIVFRGPTASAGMLSSQHSQAFES
                     WYANCPGLKVIVPSNPKDAKGLLKAAIRDDDPVIFMESEQMYGDKGEVPEGEYLMPIG
                     VADIKRPGNDVTIVSFGKIIKTAYKAAEELAKENIDVEIIDLRTVRPIDYATVIESVK
                     KTNRMVIVEESWPLANIATEVAFKVQREAFDYLDAPVLRVTGGDVPLPYAPTLIDAYL
                     PNPERIIRAVKKVMYLD"
BASE COUNT          794 a          459 c          531 g          753 t
ORIGIN      
        1 aaagcatcac caacaaaagc ggtgtggcat ataatcctga ctttaatatt caggaagcta
       61 tcagaaaaat tgaaggcatg cagatttata atggttcaat gacctactgg cccggtagtg
      121 taattgagaa ttggtgggca actgcctatg ctttgcattt tctgactgaa gcaagaaagg
      181 caggatatgc agttaatgaa aataccatca acagaacttt tgaatatctg aagtcgaaag
      241 taaagactaa agaaacagag acagtatatt ttgctaatgc aaacaacgtt atcgaaaaac
      301 aagtgagagt aaaacgggaa atcatttact cactttatct gatggcactt aacgatagaa
      361 gagatttgtc tatgatgaat tattataaag caaatcattc gcaacttaca ttagattcaa
      421 aatacatgct tgccctaagc tatcttgcaa ttggcgatac aaaaagttat ttggcactgc
      481 tacccgaaaa atttgcagga gaaaaatcac aacgtagtct gtcaggcaat ttctcatctt
      541 acatcagaga tcaggcattg gcattaaatt gcttgttgga aacagacccc gacaatgcac
      601 agattccaac tatggcacgc acactttcgc aattgataag aggagaaaaa tggttaagca
      661 ctcaggaacg tgcatttgca ttacttgcat tgggcaagtt tgctaaacgc agcacttctg
      721 caaatgttcg tgcaactgta tttgctgatg gaaaacaaat aggcaccttt aatggaaatg
      781 atttagtgat aaataacaat cttgccggaa agaaaattga aatccggaca gaaggcaaag
      841 gtcagttgta ttattttctt gaagtgagtg gaattaaaga aagtttaagg ttgaaagatg
      901 aggatagtaa attgaatgtc aggagaagtt acttcaatcg ctttggtcag caaattaata
      961 acaatttcaa acaaggtgat ttgattgtag tgcagttggc attaagttct cctaccggca
     1021 ttgatgtaga gaatgttgta ctcacagact taattccttc aggatttgaa attgaaaatc
     1081 cacgaattgt tgacgacaga gaaatgactt ggataaaaag taaatcacag ccggattatt
     1141 tcgatatccg tgatgacaga attaatctat atactcaggc aggtaaaaca ataaagtatt
     1201 tttattatac cgtcagagct gtcaatacag gaagttttgt tgtggggccg gtgagtgctg
     1261 atgccatgta tgatggcgag tttcattctt atagcggagg tggaagagtt aaagtagtta
     1321 aatgaagtca gcaattaaag gcttccgaag tgctgtttta aaaagtgcaa ttgtttttgc
     1381 agtatgcatg ttgttggtgt atttgttggc atggttgatt ccattcaatg taaacattcc
     1441 ttatgctgtt acagttactg acagtcgtgg cgaagtgctg catgcttttt tgtctaaaga
     1501 tgaaaagtgg cggatgaaag caaacttgaa tgaaataaat ccacaattgg taaaagctat
     1561 aattcaaaag gaagaccgtt ggttttattt tcattatggc gtaaatcccg tagccgttgg
     1621 cagggcagta gtgaataatt taatttatca gcgaaaaact tccggtgcat caaccatcac
     1681 catgcaggtg tcaagattgc tatgggaacg attacttttt gttgtacaaa actttgcctg
     1741 taaacttctt tattcagtac attaaacaaa cgtaaattgt atgatgttcg tacagccttt
     1801 acctgatata caccctgtgc taaatttgtt tagtccaaat acatcacctt cttcacagcc
     1861 ctgataatac gttcggggtt tgggaggtag gcatcaataa gtgtaggggc atagggcaat
     1921 ggcacatcgc cacctgttac acgcagcacc ggtgcatcaa gataatcgaa tgcttcgcgc
     1981 tgtactttaa atgcaacttc tgtagcaatg tttgccagcg gccacgattc ttcaacaatt
     2041 accatgcgat tggttttttt aaccgactca ataactgttg catagtcaat aggacgcacc
     2101 gtacgcaggt caataatttc aacgtctata ttttctttgg ctaactcttc tgctgcctta
     2161 tatgcagtct ttataatttt tccgaacgat acaatggtaa catcattgcc aggacgtttg
     2221 atgtctgcaa cgccaatagg catcaggtat tcaccttcag gcacttcgcc tttgtcgcca
     2281 tacatttgtt cgctttccat aaaaattaca ggatcgtcat cacgtattgc ggctttaaga
     2341 agtcctttgg catctttagg gtttgaggga acaataactt ttagtccggg gcagttggca
     2401 taccaacttt caaatgcctg tgagtgttgt gaactgagca tacctgccga tgcagtaggc
     2461 ccgcgaaaaa caataggtac tccatattgt ccgccactca tacttttcat tttagctgct
     2521 gcattaataa cctgatc
//
LOCUS       DAOOOI010000195         2405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_7033_length_2405_cov_12.930213, whole genome shotgun sequence.
ACCESSION   DAOOOI010000195 DAOOOI010000000
VERSION     DAOOOI010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7033_length_2405_cov_12.930213"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="PKX59_11770"
     CDS             <1..1143
                     /locus_tag="PKX59_11770"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOZ91574.1"
                     /translation="KLVNKKNTYSLSGGLDYSINFITDPEGINSDKKTDGLRHYLAIK
                     KTSGTIRWGLSQVGVNPTYDPTDFGYYVTPNRINSNANITFFRFKPYGSVQEGNWRFD
                     LNYSQHYKTGYMREMSLQTNLFILFKSYNAIFGGGGFTPAAGREYDPRLNGRYVNALK
                     YWFAYIGVSTDYRRALALDMEINISNFIKTYLSEGGSADATLRYRINDKLTVNASSEY
                     NFDPYNFGFTSETDPNIYLFGLRRTNTYINSLAARYIFKNDLSLSIVARHYWFNYAYR
                     KYFLLQDDGGLAVTTDIYNNEFDGSYNFFNADLLFSWRFAPGSTLTFSYKNIFDNGTD
                     GKLETASFTKNLDYVFKNPHVQTFSIKVLYYLDYLQLRKNNISSEN"
     gene            1195..>2405
                     /locus_tag="PKX59_11775"
     CDS             1195..>2405
                     /locus_tag="PKX59_11775"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ91575.1"
                     /translation="MKFILIILAGITLLTKPVMGQTIQAKITDNNNEPLTGAIAELRL
                     TKDSTLNKVAVADATGKVIFDQIKPGSYFIKTTFLGFNNYFSPAFEFTEGENKDFRTI
                     KLSSSSTNLKQATITAFKPLVEVKADKTVFNVENSINSTGSTALELLQKAPGVVVDNN
                     DNIILKGRGGVMVQIDGREIRMTESELADYLRSIQSTDVESIEIISNPSSKYDAQGTA
                     GIINIKLKKNKNYGTNGSITAGYAVGTYSKYNTSLSVNNRTKKFNIYSTYSNNWGDRK
                     NEFYLYREQNPFTFNQSSKSKRGGLSHNYKAGADYQMNAKNSFGIMINGNYSDITGHQ
                     RNRNYISNFYSGVTDSILISNQRYNSINNNFYANLNHHYKDTTGTELTTDFDYGYYNG
                     TRNTYQPNEYT"
BASE COUNT          864 a          432 c          404 g          705 t
ORIGIN      
        1 aaattagtga ataaaaaaaa tacctatagt ttaagtggcg gacttgatta cagtattaat
       61 tttattacag atcctgaagg aataaattct gataaaaaaa ctgatgggtt acgacattat
      121 cttgctataa aaaaaaccag tggaacaatt cgctgggggc tttcgcaggt tggtgttaat
      181 cctacttatg acccaacaga ttttggctac tatgttacac caaacagaat taattccaat
      241 gctaatatta catttttccg attcaagcct tatggttctg tacaggaagg taattggcgt
      301 tttgacctaa actattcaca gcattataaa acaggctaca tgagagaaat gtctttacag
      361 acaaatcttt ttatactatt taaatcgtac aatgcaattt ttggtggtgg tggatttacc
      421 cctgcagccg gacgtgaata tgatccaaga ctaaatgggc gttatgttaa tgcgttaaaa
      481 tactggtttg catatatcgg agttagtaca gattacagaa gggcattagc attagatatg
      541 gaaattaata tcagtaactt tattaaaacc tatctttccg aaggcggaag cgcggatgct
      601 accttacgtt atcgcattaa tgacaaactc acagttaatg cttcttcgga atacaatttt
      661 gatccctata atttcggttt tacaagtgaa actgacccga atatttacct ttttgggtta
      721 agaagaacca atacctatat aaacagtctt gcagcacgat atatatttaa aaatgatctg
      781 tcgctgtcaa ttgttgcacg gcattactgg tttaattacg catataggaa atactttttg
      841 ctgcaggatg acggtgggct tgctgtcaca accgatattt acaacaacga atttgatggc
      901 agctataatt tttttaatgc tgaccttttg ttttcatggc gatttgctcc cggcagcaca
      961 ttgacttttt cgtacaaaaa catttttgac aatggtactg atggcaaact tgaaactgca
     1021 tcattcacta aaaatcttga ttatgttttt aaaaatccgc atgttcaaac attttcaatt
     1081 aaagtacttt attatctcga ttatttacaa ttaagaaaaa ataatatttc atcagaaaac
     1141 taaataatac atagcagcaa tactatattt tcccgcaacc ataacacaca aacaatgaaa
     1201 tttattctaa ttatcttggc aggaataacc ctgttgacaa aacctgtaat gggtcaaact
     1261 attcaggcta aaatcacaga caataataac gagccgctta caggtgccat tgcagaactg
     1321 cgactcacta aagattcaac cttaaacaaa gttgcagtag ctgacgctac aggaaaagta
     1381 attttcgacc agataaaacc cggtagctat tttataaaga caacgttttt gggattcaac
     1441 aattatttta gtcctgcatt tgaatttaca gaaggagaaa acaaagattt tcgcactata
     1501 aaactttcat catcatcaac taatcttaag caagcaacca tcactgcatt taaacccttg
     1561 gttgaagtta aagcagataa aactgtattt aatgtagaaa acagtattaa ctcaacagga
     1621 agtactgcac ttgaattgct tcaaaaagcg cccggtgttg ttgtagataa taacgataac
     1681 attattttaa aaggacgtgg tggcgtgatg gttcagattg atggacgtga gattagaatg
     1741 acagaaagcg aacttgctga ctaccttcgt tctattcaaa gtacagatgt ggaaagtatt
     1801 gaaattattt caaacccatc atccaaatat gatgcgcaag gcacggccgg aatcatcaat
     1861 attaaactaa agaaaaacaa aaactatggc accaacggaa gtataacagc aggttatgcc
     1921 gttggaacat attctaaata caacacctca ctttcagtca acaacagaac aaaaaagttt
     1981 aatatttact ctacttacag taataattgg ggtgacagaa aaaacgagtt ctatctgtat
     2041 cgcgaacaaa acccgtttac attcaatcaa tcatcaaaat cgaaacgtgg tggcctaagt
     2101 cataattaca aagccggtgc agattatcag atgaatgcta aaaatagttt tggcattatg
     2161 ataaatggaa attacagcga cataaccgga catcagagaa acagaaacta tatcagcaat
     2221 ttttattcag gagtaacaga cagtatcctt atttccaatc aacgttataa ctctataaac
     2281 aataatttct atgccaatct aaatcatcat tacaaagaca caacaggaac agagttgaca
     2341 acagactttg attacggcta ctacaacgga acaagaaaca cgtatcaacc aaatgaatac
     2401 acatt
//
LOCUS       DAOOOI010000196         2403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_7041_length_2403_cov_4.000852, whole genome shotgun sequence.
ACCESSION   DAOOOI010000196 DAOOOI010000000
VERSION     DAOOOI010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7041_length_2403_cov_4.000852"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="PKX59_11780"
     CDS             <1..1037
                     /locus_tag="PKX59_11780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOZ91576.1"
                     /translation="LGCKDTVTKTINIQQFMSATATTIQNVRCKGECNGSGLVAVTNN
                     NGPYTILWSNPPGQSNDTIVNLCQGNYSGTVTDSAGCTSNFSLNISEPPQLTLSATAT
                     PDYCSGQCIGTATALAGGGTPPYNFVWSNGSNSGSLNQLCAGNYTVSVTDSKGCTTSP
                     VTVSVLFSDSLAALDVVPSIDTIYRGQSVNLSATQNTGYIYNWQPPTYLNSNGIANPV
                     STPPVDIEYILSITDAFGCKINDTAKIVVIQYRCEEPEIYIPNSFSPNGDRVNDEIYV
                     YGGQIKELLFRIYDRWGEKVFETKQPGQGWDGFYRGKRVTPGVFVYYVEATCYDNQKF
                     FKKGNITVIR"
     gene            1041..2051
                     /locus_tag="PKX59_11785"
     CDS             1041..2051
                     /locus_tag="PKX59_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOZ91577.1"
                     /translation="MKNLLSGIFTICLFCLNAAGQDVHFSQFDFSPLHQSAAQTGNFK
                     GDYRLTAINRVQYKSVTVPYKSLGASVDMNIAKTDEKKSWWSAGLLFENDVTGDADYK
                     TTIVDVSVAWNSFLDENKKHQLTLAIQPGWMQNGLNFSELYFGSQYDGDIFNPNLPTG
                     ESPARSSYSNFNLNAGAGYKYAFSENSDLNLIFGASHLNQPDISFLNDKAKLFTRLTG
                     VGVLNLMLSEKISLQPQVLFYRQGKFSQLNGGANVQLHSLENNFKNFRFSAGVFYRNK
                     DAVIARIGAGIGNLDAGFAYDFNTSDLKRASNGRGAYEVALSYIITKVKPLKKNPPCP
                     VY"
     gene            2102..2323
                     /locus_tag="PKX59_11790"
     CDS             2102..2323
                     /locus_tag="PKX59_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91578.1"
                     /translation="MYLLFAVQLLQAQSPTAYERAGDKAMKEGDYYSAFAFYFNSYAS
                     NNTDLVITWKLAEACRLYNDYENAQKYYN"
BASE COUNT          730 a          431 c          482 g          760 t
ORIGIN      
        1 cgctcggttg taaggatacc gttaccaaaa ctattaacat acaacaattc atgagtgcaa
       61 ctgcaactac cattcagaat gtacgttgca aaggcgaatg taacggttcc ggccttgtcg
      121 cagtaacaaa caataatggg ccatacacta tattgtggag taatcctccg ggacaatcca
      181 atgatactat tgttaatcta tgtcaaggca actattcagg tactgtaaca gattctgcag
      241 gatgtacttc aaatttttct ttaaatattt ctgaaccacc acaacttacc ttatctgcaa
      301 ctgcaactcc tgattattgc agcggtcaat gtattggcac agccacagca ttggcaggtg
      361 gaggcacacc accatacaac tttgtctgga gtaatggttc aaattcaggt tctctcaatc
      421 agctttgtgc cggcaactat actgtttctg taactgactc aaaaggttgt actacttctc
      481 ctgtaaccgt aagtgttttg ttttctgatt cgttagcagc attagatgtt gtgccttcta
      541 ttgataccat ttatcgtggt caaagtgtta acctatcagc aactcaaaat acaggttaca
      601 tttacaactg gcagccacca acttatttaa acagcaacgg tattgcaaat cctgtcagca
      661 cacctcctgt ggatattgag tatatcctta gcataacaga tgcattcggt tgtaagatta
      721 atgacactgc aaaaattgtt gtcatacaat atcgttgtga agaacctgaa atttatattc
      781 ctaattcatt tagcccgaat ggtgacagag ttaatgatga gatttatgtt tatggcggac
      841 aaataaaaga gttgctattt cgtatttacg atcgttgggg agagaaagta tttgaaacca
      901 aacagccggg tcagggttgg gatggatttt atagaggcaa acgtgtaaca cccggtgtgt
      961 ttgtttacta tgttgaggct acttgctatg acaatcagaa attctttaaa aaaggtaata
     1021 taaccgtaat acggtaagaa atgaaaaatc tcctgtcggg catatttaca atttgtttgt
     1081 tctgtttgaa tgcagcaggg caggatgtgc atttttcgca gtttgatttt tcgccattgc
     1141 atcagagtgc agcacaaacc ggaaacttta aaggcgatta cagactgact gcaattaatc
     1201 gtgtacagta taaatctgtt acagttcctt ataaatcatt aggtgcatct gttgatatga
     1261 acattgcaaa aactgatgaa aaaaaatcat ggtggagtgc aggtttatta tttgaaaatg
     1321 acgttacagg tgatgcagat tataaaacta ctattgttga tgtttctgtt gcctggaatt
     1381 cttttttaga tgaaaacaaa aaacatcagc tcaccttagc tattcagccg gggtggatgc
     1441 agaatggttt gaatttttcg gaactgtatt tcggttcgca atatgatggt gacatcttta
     1501 atcctaacct gcctacaggt gaaagcccag cgcgcagtag ctattccaat tttaatttga
     1561 atgcaggtgc cggttataaa tatgcattta gtgaaaattc tgatttgaat ttgattttcg
     1621 gtgccagcca cctgaatcaa cctgatattt catttctgaa tgataaagca aaattattta
     1681 cccgactcac aggtgttgga gttttaaacc tgatgctttc cgaaaaaatt tctctgcaac
     1741 cacaagtgtt attttatcgt cagggaaaat tctcacagtt aaatggcggt gcaaatgttc
     1801 aactacattc attagagaat aattttaaaa atttcagatt ctcggcagga gtattttatc
     1861 gtaacaaaga tgctgtcatt gcacgtattg gagcaggtat tggcaattta gatgccggtt
     1921 ttgcatacga tttcaataca tctgacctga aaagggcaag taacggcaga ggcgcttacg
     1981 aagtagcatt atcttacatc atcaccaaag taaaaccatt aaagaagaat ccaccatgcc
     2041 ccgtttattg atatattttt tcggcagcag gaggattgtt gttgcaaaga ttgtaatgtt
     2101 aatgtacttg ttatttgctg tacagttgtt gcaggcgcaa tctccaacag cctacgagcg
     2161 tgcaggtgat aaggctatga aagaaggtga ttattattct gcattcgcat tctattttaa
     2221 ttcttatgca agcaataata cagatttagt tataacctgg aaactggcag aagcatgccg
     2281 gctttacaat gattatgaaa atgcacagaa gtattataac taggcttatg gattagataa
     2341 actaggtctg tttcctttta ttgcttttta tctggcttag attaatcagc gacagggtta
     2401 tta
//
LOCUS       DAOOOI010000197         2373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_7131_length_2373_cov_23.172131, whole genome shotgun sequence.
ACCESSION   DAOOOI010000197 DAOOOI010000000
VERSION     DAOOOI010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7131_length_2373_cov_23.172131"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PKX59_11795"
     CDS             <1..945
                     /locus_tag="PKX59_11795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOZ91579.1"
                     /translation="IAGFFAACYHAFYPYLFTYSFSIYDTTLFVFLLVLTIELLLTEK
                     LSSKRLVLAGIIIGLAFLTRATIITFLPGLFLYVFYRYFKSQNVTAAFISCSLIVAGC
                     LLAMAPWLVRNYSLTGKLLISTHGSFGMWQGNNPLSFEYLKANKSLDEIYRLNPPLPI
                     YAQYPLAARTPDEAVKVSAAYQQEAVNWIKNNFPEFVSMGWLKAEKLWSWNRNPDSKS
                     VKFGSNEGRKSVYLISYLPILLLFPFGLILLFKKHPVEAFTLLLILLAFTGAHSIAIG
                     FTRARIPIDFFLMLCSGITITWLLQRFFPSLYLKFKSS"
     gene            942..2051
                     /locus_tag="PKX59_11800"
     CDS             942..2051
                     /locus_tag="PKX59_11800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOZ91580.1"
                     /translation="MSDKKYTVIFLGKLPPPYIGPSVACEIILNSKLKDEFNLIHLDL
                     SDKRDINTLAKLDLTNFYLAFKQYFQLFWYCIRYRPDLVYVPAGQTNVGYTRDAGYIL
                     IAKLFGIKVLTHLRGGYFLNWYNESGKTMKSFIRFVHKKVNGQIVLGNNLRKLFNWIV
                     PDEKIFVIPNGGNFPVNYNERNNNKVKVLFLGNYVRTKGVLEALYAAPLVYEKNKNVE
                     FVFAGEWREADVKTDFKKFLQQHPELPVINHGPVKGDKKFSILNEADIFVFPTYYRNE
                     GHPWVTVEAMAAGLPVISTDHAAISESVHDGKNGYLVEKKNIQQLAEKIIILANDKDL
                     RNKMGAESRRIYLENLTEEKMVERMGHAFRSVIEN"
     gene            2063..>2373
                     /locus_tag="PKX59_11805"
     CDS             2063..>2373
                     /locus_tag="PKX59_11805"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOZ91581.1"
                     /translation="MKKVLVTGGAGFIGSNLCDALLTNDLYEVHCLDVFDDFYNPAVK
                     RNNISEALKSKNFFLHEGDIRDTDFLEKLFQQPFDIIVHLAARAGVRPSIEKPLLYED
                     V"
BASE COUNT          766 a          417 c          424 g          766 t
ORIGIN      
        1 attgcaggat tttttgcagc atgttatcat gctttttatc catatctgtt tacatattcg
       61 ttttctatct acgacactac actttttgtt tttttacttg tattgactat tgaattatta
      121 ctcacagaaa aactttcatc aaaaagactt gtacttgccg gaattataat cggtcttgca
      181 tttttaacac gagcaacaat cataactttt cttcccggct tatttctcta tgtattctat
      241 cgctacttca aatcacaaaa tgtaacagca gcatttatca gttgctcatt aattgttgca
      301 ggatgtctat tggcaatggc accctggttg gttagaaatt attcactaac aggaaaatta
      361 ttaatcagca cacatggttc attcggtatg tggcaaggca acaatccttt gtcatttgaa
      421 tatctaaaag ctaacaaatc actagacgaa atttatcgtt taaacccacc gttgccaata
      481 tatgcacagt atccacttgc agcacgcaca cctgatgagg ccgtaaaagt atctgcagcc
      541 tatcaacaag aagctgtaaa ttggataaaa aacaatttcc ctgaatttgt ttctatgggt
      601 tggctaaaag cagaaaaact ctggagttgg aatcgtaacc ctgatagtaa aagcgtgaaa
      661 ttcggaagca atgaaggacg aaaatctgtt tacctgattt catatctgcc tatattacta
      721 ctctttccat ttggattgat attattgttt aaaaaacatc cggttgaggc atttacgttg
      781 ttattgattt tacttgcctt caccggtgca cattcaattg ctattggatt caccagagca
      841 cgcataccta ttgatttttt tctgatgtta tgcagtggca taaccataac ctggttgctg
      901 caacgctttt ttccatctct ttatcttaaa tttaaatcat catgagtgac aaaaaatata
      961 ctgtaatttt tctgggcaaa ctgcctcctc cctacattgg cccatctgtt gcctgcgaaa
     1021 ttattctcaa ctcaaaactt aaagacgaat ttaatctgat tcatcttgac ctttctgata
     1081 aacgtgacat caatacatta gcaaaactcg atttaacgaa tttttattta gcctttaaac
     1141 aatattttca gttgttctgg tattgtatcc gctatcgccc tgatctggtt tatgtacctg
     1201 caggacaaac caatgttggc tatacacgtg atgcaggata tattcttatt gcaaaacttt
     1261 ttggcattaa agtacttaca catcttcgcg gtggatattt tctgaactgg tataatgaaa
     1321 gtggaaaaac gatgaaatca ttcattcgtt ttgtacataa aaaagtaaac ggacagattg
     1381 tgcttggcaa caacctgcgt aaactattta actggattgt acctgacgaa aaaatatttg
     1441 ttattcccaa tggtggaaat tttccggtaa actacaatga gcgaaacaac aataaggtta
     1501 aagtactttt tcttggtaac tatgtgcgca ccaaaggagt attggaagca ttgtacgcag
     1561 caccattagt atatgaaaaa aataaaaacg tagaatttgt atttgccggt gaatggcgtg
     1621 aagcagatgt aaaaacagat tttaaaaaat ttttacaaca acatcctgaa ctgcctgtga
     1681 taaatcacgg gcctgttaaa ggtgataaaa agttcagcat cctgaacgaa gccgatattt
     1741 ttgttttccc tacatactat cgcaatgaag gtcatccatg ggttactgtt gaagctatgg
     1801 ctgccggact gcctgtaatt tctacagacc atgcagcaat ctccgaaagc gtacatgatg
     1861 gaaaaaatgg ttatctggta gaaaagaaaa atattcagca attagccgaa aaaataatta
     1921 ttttggcaaa cgataaagat ttgagaaata aaatgggagc agaaagcaga cgcatctatt
     1981 tagaaaatct gacagaagaa aaaatggttg aacggatggg tcatgcattc agaagtgtaa
     2041 ttgagaatta aatattattg aattgaaaaa agtattagta acaggcggag caggttttat
     2101 tggcagcaat ttatgtgatg ctcttttaac taatgattta tacgaagtgc attgccttga
     2161 tgtgtttgat gatttttaca atcctgctgt aaaaagaaat aatatttcgg aagcattaaa
     2221 gagtaaaaac ttttttttgc atgaaggtga tattcgtgat actgactttc tcgaaaaact
     2281 atttcaacaa ccgttcgaca ttatagttca tctggctgca cgtgccggag ttagaccgag
     2341 tattgaaaaa cctttgcttt atgaagatgt aaa
//
LOCUS       DAOOOI010000198         2359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_7171_length_2359_cov_6.216580, whole genome shotgun sequence.
ACCESSION   DAOOOI010000198 DAOOOI010000000
VERSION     DAOOOI010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7171_length_2359_cov_6.216580"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1965
                     /gene="pbpC"
                     /locus_tag="PKX59_11810"
     CDS             <1..1965
                     /gene="pbpC"
                     /locus_tag="PKX59_11810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOZ91582.1"
                     /translation="ITMQVSRLLSPAPRTLFNKMFEAFRAVQLENRYSKKEILEMYLN
                     LIPYGGNIEGVKAASVLYLDKLPADLSLAECVTLCIIPNRPNSLRPGYDNDLIRRERN
                     KWLTRFEKNKVFKKEIINDALNEPVEMKRQAAPALITQLAYRLKEQYPTIENIHSTLQ
                     APLQHKVQSLVYNYSKRKSLQSIYNASAIVIDNKNHKVVAYIGSADFKDQLHHGQVDG
                     VNAVRSPGSSLKPLIYAMAFDKGLATPMTVINDVPVNYMGYNPENYNSKFNGRVTVDF
                     ALSHSLNIPAVKILNQLGVPTVTQALIKSGFSSIEKSKNKLGLSLALGGCGVRLFELT
                     NMYAAFANNGVYQKLKYVEADSLNSDTIRIISPGAAYLTTEILSKITRPDLPDNYANL
                     LRIPHIAWKTGTSYGRRDAWSIGYNKRFTVGVWLGNYNGSGVAELSGASVATPLLFDI
                     FNAIDYDSDSTWFSPPADVNFRLVCSETGMIPGVNCANEIMDEFLPGISSNAICTHIK
                     EYIISPDEKISYCTSCMPAAGYKKKLYPVPDAEYLAFLKSENISLNLPPPHNPLCTRV
                     FTGQPPKIVSPNMQSEYLLDLKGGDKIMLQCQASADVQNVYWYINDLFYRAASANEQL
                     FCSPPKGRVKISCADDMGRNSDIFIKIEQM"
     gene            complement(1967..>2359)
                     /locus_tag="PKX59_11815"
     CDS             complement(1967..>2359)
                     /locus_tag="PKX59_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91583.1"
                     /translation="TEQAARIIGSYKHGITKVKLEIVQPPENTDSLEKYFMQEQVIDA
                     EGKVVNPTGYTISIWRTRDFDHALLLTKYLQQEEYIQSFYVGKKYQNGRPLYHILVLN
                     ISTQEEAVKLKDFWERKGFMRVRMLEKF"
BASE COUNT          748 a          426 c          454 g          731 t
ORIGIN      
        1 atcaccatgc aggtgtcaag attgcttagt cctgcaccac gcaccttgtt taacaaaatg
       61 tttgaagcat tccgtgcagt acaattggaa aacaggtatt caaagaaaga aattttagag
      121 atgtatctca atctgattcc ctatggtgga aatattgaag gggtaaaagc tgcatcagtt
      181 ttgtatctcg acaaattgcc tgcagactta tctcttgcag agtgtgtaac attgtgtatt
      241 attcctaaca gaccaaactc tctgcgcccg ggttatgata atgatctcat caggcgcgaa
      301 agaaacaaat ggctgacaag atttgaaaag aataaagttt ttaaaaaaga aatcatcaac
      361 gatgcattaa atgaacctgt tgaaatgaag cgacaggctg cacctgcatt aattacacaa
      421 ttagcatata ggctaaagga acagtatcca accattgaga atattcattc tactttacaa
      481 gcaccattac agcataaagt acaatcatta gtgtataact acagtaagcg taagtcactg
      541 caaagtattt acaatgcatc agcaattgtg attgacaata aaaatcataa agtagtagcc
      601 tatattggct ctgcagattt taaagatcag ttacatcatg gacaggttga tggtgtaaat
      661 gctgttcgtt ctcccggcag tagcctcaaa ccattaatct atgcaatggc ttttgacaaa
      721 ggtttggcaa cacctatgac agtaattaat gatgtgcctg taaattatat gggatataat
      781 cctgaaaact acaattcaaa atttaatggc agagttactg tagattttgc tttatcacat
      841 tcactgaata ttcctgcagt aaaaattctg aatcaattgg gggtgcctac tgtaacacag
      901 gcattaatta aaagcggttt tagctcaatt gaaaaaagta aaaacaaact gggtttgtct
      961 ttggcacttg gaggttgtgg tgtgcgtctt tttgaactca caaacatgta tgctgccttt
     1021 gcaaataatg gagtctatca gaaattaaaa tatgttgaag cggattcatt gaatagtgac
     1081 accataagaa taatatctcc cggtgcagcc tatttgacca ctgaaatact cagtaaaatt
     1141 acacgacctg atttacctga taattatgcc aacctacttc gtattcctca tattgcttgg
     1201 aaaaccggaa cttcgtatgg taggagggat gcatggagta ttggctacaa caagaggttt
     1261 acagtagggg tgtggcttgg aaattataac ggtagtggcg ttgccgaact tagcggtgca
     1321 tcagttgcaa ctccattgct ctttgatata tttaatgcaa tagattatga tagtgattca
     1381 acatggttca gcccgcctgc tgatgtaaat ttcagattgg tttgcagcga aacgggaatg
     1441 attccaggtg tcaattgcgc aaatgagatt atggatgagt ttttacccgg tatttcatcc
     1501 aatgcaattt gtactcatat aaaggaatat atcatttcac ctgatgaaaa aataagttac
     1561 tgtacatcat gcatgcctgc tgcaggttat aagaagaaac tttatccggt tcccgatgca
     1621 gaatatttag catttctgaa aagtgaaaac atcagtctta atcttccacc accacataat
     1681 cctttatgca caagagtttt tacggggcag ccaccaaaaa ttgtttctcc caatatgcaa
     1741 agtgaatatt tgcttgattt aaaaggagga gataaaatta tgcttcaatg tcaggcatct
     1801 gctgatgtgc agaatgttta ctggtacatt aatgatttgt tttatcgtgc tgcttcagct
     1861 aatgagcaat tgttttgttc tccaccaaag ggtcgtgtta aaatttcttg tgctgatgat
     1921 atgggtcgca acagtgacat ctttattaaa atagaacaga tgtaattcag aatttttcca
     1981 gcatccttac acgcataaaa ccttttcgct cccaaaaatc cttcaactta acagcttctt
     2041 cctgtgtaga aatgtttaag actaaaatat ggtaaagtgg tctgccattc tgatattttt
     2101 tgccgacata aaaagattga atgtattctt cctgttgcaa atattttgtc agtaaaagcg
     2161 catgatcaaa atcgcgtgtg cgccaaatac tgatggtgta accggttgga ttaacaacct
     2221 taccttcagc atcaataact tgttcctgca taaaatattt ttcaagagag tcagtatttt
     2281 caggcggttg aactatttca agcttcacct ttgttatgcc atgtttatag cttccgataa
     2341 tacgtgcagc ctgttcagt
//
LOCUS       DAOOOI010000199         2337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_7249_length_2337_cov_9.249343, whole genome shotgun sequence.
ACCESSION   DAOOOI010000199 DAOOOI010000000
VERSION     DAOOOI010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7249_length_2337_cov_9.249343"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..>2337)
                     /locus_tag="PKX59_11820"
     CDS             complement(218..>2337)
                     /locus_tag="PKX59_11820"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOZ91584.1"
                     /translation="FILQGAFAQDLSKINFTLAKKLSGPFSVTRPVALFAQGDVGIIQ
                     NYVQQNSGKVKFVAGDIVSLVLPLGAVNGLIALPSVEAVEDNGMQLVALDDSMRVRNN
                     INPVQNGMAPLPQGYDGSGVVMGFIDSGIDFSHPDFKHANGSTRVLYIWDHNLTVGNA
                     PMPYNYGTEFTAADIDAGNASAHVDNSNGHGTHVTGMGAGNGLALNDHKGAAPAADII
                     SVCVNWNLSDNDWLTTVADAVNYIYSKAYALNKPCVINISAGTYYGSHDGKDLQAQAI
                     SNLISQRNGRSLVCAAGNAGNIPIHVQHLHTAANDTSFTWFNFNPAYGNSIYLEMWAD
                     VANFTQMKFAVGADNVTSGYELQTMGNYYTVSQNLGTIHTDTLWGVSGNRIALVQRFA
                     SLSNGRYSMIFNIIPDSTQYRFRLSSTQTGKFDLWSFQMTPSNALPTPQQFPEIVKYV
                     SPDYDQTMVSSFSCSDKVITVGEHKNRMTYMDCTNNLFVSTTNYTPGALAPQSSHGPT
                     RDARLKPEICASGGMSIAAGAYNVLPNLPVTSKALGCMHIRDGGTSTASPVVAGIAAL
                     ILQRYPNASWQDIKNCITQNAMVDSLVTGTIPNNSWGYGRADGFAAMLGCSFLSINDY
                     DSQIGSIYPIPFNQSFQLTAVKQLSAVNIFNPLGQLVYQSSFNIEATHKITINPILPS
                     KGVYFAQLISTEGYKQTIKLIYQ"
BASE COUNT          744 a          449 c          434 g          710 t
ORIGIN      
        1 gtataaatat agaacaatga aaaacttaca taaaatttac cggaaaatta gcatttaatt
       61 ttacagttat ttaacttgaa gtaaaattta acaattgata gttctaaaaa tgtattatgt
      121 agaattttac ctaaagcaaa tacttaataa aacatatctg cttgattagt caattatctt
      181 aagtttcttt ggggttaagt ttgatgtttt aaaatgtcta ttgatatatc aattttatcg
      241 tctgcttata accttcagta gaaatcaatt gagcaaaata aacgcctttt gaaggtaata
      301 tagggttaat tgttatctta tgcgttgctt cgatattgaa agatgattga taaaccaact
      361 gtcctaatgg gttaaaaata tttactgctg aaagttgttt cacagccgtt agctgaaatg
      421 actgattaaa aggaattgga taaatacttc caatttgtga atcataatca ttgattgaaa
      481 gaaaactaca accaagcatg gcagcaaatc catctgcacg gccatatccc cagctgttgt
      541 ttggtattgt acctgtaacc aatgagtcaa ccattgcatt ttgtgtgata cagtttttaa
      601 tatcctgcca cgatgcattt gggtatcgtt gaagtatcag tgctgcaata ccggcaacta
      661 caggcgatgc tgtagaagta cctccatctc tgatgtgcat gcaacctaaa gccttacttg
      721 tgaccggaag attgggtaac acattataag cacctgctgc aatagacatt ccaccgctgg
      781 cacaaatttc aggtttgaga cgtgcatcac gagtagggcc atgactgctt tgtggcgcca
      841 atgctcccgg tgtatagttt gttgttgaaa caaaaagatt attggtgcag tccatataag
      901 tcatgcggtt tttatgttca ccaacagtaa ttactttatc actgcatgaa aaactactta
      961 ccatagtctg gtcataatcg ggagaaacat attttacaat ttcaggaaat tgttgcggtg
     1021 ttggtagtgc atttgatggt gtcatctgaa aactccataa atcaaatttg cctgtttgcg
     1081 ttgaagagag tctgaagcga tattgagtgc tgtccggaat aatattaaat atcatactat
     1141 agcgtccatt agaaagtgaa gcaaaacgct gcacaagcgc aattcggttt cctgaaacac
     1201 cccacaatgt atctgtgtgt attgttccca aattttgaga aacagtataa tagttaccca
     1261 ttgtctgtaa ttcataaccg gaagttacat tatctgcacc gactgcaaat ttcatctgag
     1321 taaagtttgc cacatcagcc cacatttcta aataaataga attaccatag gcagggttaa
     1381 aattaaacca tgtaaacgat gtatcattag cagcagtatg caaatgctga acgtgaatag
     1441 gtatatttcc tgcattaccg gctgcacaaa caagtgagcg gccattacgt tgggaaatga
     1501 gattagagat agcctgtgcc tgtaaatctt ttccatcatg agaaccataa taagttcccg
     1561 cactgatgtt gattacacat ggcttattaa gtgcataagc ttttgaataa atataattca
     1621 cagcatcggc aactgttgtt aaccaatcat tgtctgaaag gttccagttt acacaaacag
     1681 aaattatatc ggcagcaggt gcagcaccct tatggtcgtt caatgcaaga ccattgccgg
     1741 cacccatacc tgtaacgtgt gtgccatgtc cattgctgtt gtcaacatga gcggaggcat
     1801 tgcctgcatc aatatctgct gcagtaaatt ctgttccata attatagggc attggtgcat
     1861 ttccaactgt taaattatga tcccagatat acaacacacg tgtacttccg ttagcatgtt
     1921 tgaaatcagg atgagaaaaa tcaatacctg aatcaataaa tcccattact acgccactgc
     1981 catcgtagcc ttgcggcaaa ggagccatac cattttgaac aggattgatg ttgttgcgca
     2041 cacgcataga gtcgtctaaa gctacaagct gcattccgtt atcctcaact gcttctacac
     2101 ttggaagtgc aattaatcca ttaacagcac ctaagggtaa aaccaatgaa actatatctc
     2161 cggctacaaa ctttacttta ccgctatttt gttgaacata attctgaata atgccaacat
     2221 ctccttgtgc aaataatgct acagggcgtg ttactgaaaa cggcccggac aattttttag
     2281 caagtgtaaa atttatcttg ctcaaatcct gagcgaatgc accctgaaga ataaata
//
LOCUS       DAOOOI010000200         2327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_7285_length_2327_cov_9.133803, whole genome shotgun sequence.
ACCESSION   DAOOOI010000200 DAOOOI010000000
VERSION     DAOOOI010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7285_length_2327_cov_9.133803"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PKX59_11825"
     CDS             complement(<1..606)
                     /locus_tag="PKX59_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91585.1"
                     /translation="MKYIVTLFIIFIYCNTFAQQQKVNEIWNGADAFSMDNLGNLYII
                     KESVVFKADTVGKILLTYSNNNQDRFTYVDAIDPLRIMLFADEFSIISFLDNKLALQS
                     VINLREQNFYDIKAVCNSTDGFWCYDHSQQQIFKYNFTLQKISETQPLALLINNPLTI
                     TSMCANDKWLACLNKNSGILIFDRLGSYVRSIDSKHASCIML"
     gene            complement(603..2120)
                     /locus_tag="PKX59_11830"
     CDS             complement(603..2120)
                     /locus_tag="PKX59_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOZ91586.1"
                     /translation="MAILNSIMGWIIKKRMHQIELFMKYPCEVQEELLRKLISTAEHT
                     QWGEHFDYQSIDNFKTFSERVPVVTYDQLQPQVERIMKGEQNILWPTEIKWFAKSSGT
                     TSNRSKFIPVSMESLEECHFKGGKDLLSIYFNNNPDALLFNGKLLSLGGSHSINAFHN
                     ESYYGDLSAILIQNLPFWVQIMRTPEMNIALMDKWDEKIERMAQETIKENVTNISGVP
                     SWTLVLLKRILEITGKKTIAEVWPGLELFVHGAVNFTPYREQFKQIIGKHIQYLETYN
                     ASEGFFGIQDRNDSDEMLLMLDYGIYYEFIPQKHWHEENPKTLTLSEVQIGEQYALVI
                     TTNAGLWRYKIGDTIQFTSISPYRIKISGRTAHFINAFGEELVIDNAEKAITEAGKRT
                     GATVREFTAAPVYFGNKNNGAHEWLIEFSHEPENLDEFTMALDEELKKVNSDYEAKRF
                     KDITLNKPIVHKMPVNTFYKWLEKNKKLGGQHKVPRLANHRKFVDEILNVQNNLS"
BASE COUNT          685 a          459 c          387 g          796 t
ORIGIN      
        1 tagcataata catgatgcat gttttgaatc aatacttctt acatagcttc ccagcctgtc
       61 aaaaattaaa attcctgaat tcttattcaa acatgccagc catttatcat tggcacacat
      121 gctcgttatc gttagcggat tgtttattaa taatgccaac ggttgtgttt ctgaaatttt
      181 ctgaagtgta aaattatatt tgaagatttg ttgttgtgag tgatcatagc accaaaaccc
      241 atctgtagaa ttgcaaacag cttttatgtc atagaaattt tgttctctta aattaattac
      301 cgattgcaag gctaatttat tatccaaaaa actgatgata gaaaattcat cagcaaaaag
      361 cattattcgc aatgggtcaa ttgcatcaac ataagtaaac ctgtcctgat tgttattgct
      421 gtaggtaagt aaaatttttc ctacggtatc agctttaaaa actacactct cttttataat
      481 atagaggttg ccgagattat ccatgctgaa agcatcggca ccattccaga tttcattaac
      541 tttttgctgc tgtgcaaaag tgttgcagta aatgaaaatg atgaatagtg ttacaatata
      601 tttcacgaca gattgttttg tacgtttaat atttcatcaa caaactttct gtggttggca
      661 agacgtggca ccttatgctg tcctcccaat tttttatttt tttccagcca tttatagaac
      721 gtattcacag gcattttatg cacaataggt ttgttgagcg tgatgtcctt aaatcgtttt
      781 gcctcataat cggaattaac ttttttaagc tcttcgtcaa gtgccattgt aaactcatct
      841 aagttttcag gctcatgcga aaattcaata agccactcat gtgctccatt atttttattg
      901 ccaaaataaa ctggtgcagc agtaaattca cgcactgtag ctcctgttct tttgcccgct
      961 tctgtaattg ctttttctgc attgtcaatc acaagttctt ctccaaaagc attaataaaa
     1021 tgtgcagttc gcccactgat ttttattctg taaggactga tagatgtaaa ctgaatggtg
     1081 tctccgattt tataacgcca cagaccggca ttggttgtaa tgaccaatgc atattgttct
     1141 ccgatttgaa cttcggagag tgtcaacgtt tttggatttt cctcatgcca gtgtttttga
     1201 ggaataaact catagtaaat tccatagtcg agcataagca acatttcatc agaatcgttt
     1261 ctgtcctgaa taccaaaaaa tccttcggag gcattatagg tttctaaata ctgaatgtgt
     1321 tttccgataa tctgtttaaa ctgctctctg tatggagtaa aatttacagc accatgaaca
     1381 aacagttcta aaccaggcca cacttctgct attgttttct tacctgtaat ttcaagaata
     1441 cgtttcaata acaccaatgt ccaactgggc acacctgaaa tgttagtcac attctcctta
     1501 attgtctcct gtgccatgcg ctcaattttt tcatcccatt tatccatcag tgcaatattc
     1561 atttctggtg ttcgcattat ctgtacccag aaaggaaggt tctgaatcag aatagcagaa
     1621 agatcaccgt aatacgattc gttatgaaat gcattgatgc tgtggctgcc accaagagaa
     1681 agcagtttgc cgttgaatag taaggcatca gggttgttgt taaaataaat tgaaagcagg
     1741 tctttaccgc ctttaaaatg acattcttcc agcgactcca tgctaaccgg aataaactta
     1801 cttctgtttg aagttgttcc cgatgatttg gcaaaccatt ttatttctgt tggccacaga
     1861 atattttgtt cgcctttcat gattctttcc acctgtggct gcaactgatc ataagtaaca
     1921 acaggcacac gctcactaaa ggttttaaaa ttgtctatag attgatagtc gaaatgttcg
     1981 ccccactgtg tatgctctgc tgttgaaata agttttctaa gcaactcttc ctgaacctcg
     2041 catggatatt tcataaaaag ctcaatctga tgcatacgct ttttaattat ccatcccatt
     2101 atcgaattca gtattgccat atacagtgct ttgcgaacgg aaagataaga aaaattcctg
     2161 caacttccgt tttgcaaaat taattcttca acttaaatga acttaatctg taattaattt
     2221 ctttcagaaa tgtttgatgt actttcgttc catgcaaaaa tcaacaatag atttcaggtt
     2281 aataaaatta gagcgcaacg gttatcaccc cgtttgcact gttgcca
//
LOCUS       DAOOOI010000201         2299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_7364_length_2299_cov_48.563725, whole genome shotgun sequence.
ACCESSION   DAOOOI010000201 DAOOOI010000000
VERSION     DAOOOI010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7364_length_2299_cov_48.563725"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..354)
                     /locus_tag="PKX59_11835"
     CDS             complement(79..354)
                     /locus_tag="PKX59_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91587.1"
                     /translation="MRNDIKCNNDIYLSLMNAYKHRMIWQDERFTFDFYGYGGNKGGL
                     ASVRTEEVEKNDFLLLKEMFGDAVATKKGTWDKKPKKPYELVIKLPY"
     gene            complement(403..825)
                     /locus_tag="PKX59_11840"
     CDS             complement(403..825)
                     /locus_tag="PKX59_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91588.1"
                     /translation="MGGEIDIKVVIPTAGRYDTITTHKVVDNCILCVPEEQEKFYRVS
                     CPDTEIVTHPPLVGLGAKRNWIYEKFGHVFMLDDDCKGMRRMYSENKEQDKVDSKTAY
                     DLIQFTGNMCHMAGLYVFTFNRYAQPKNYHGHSPIKLK"
     gene            complement(810..1484)
                     /locus_tag="PKX59_11845"
     CDS             complement(810..1484)
                     /locus_tag="PKX59_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91589.1"
                     /translation="MAKNNGNNKKEQTPPAEQEDLQLKKMKISDLVHDAKNANKGTDN
                     GKAMIKKSIESNGLGRSILIDKNNQIIAGNKTTSNAEEQGYDDVVVVETTGNTLVAVK
                     RMDIDLDTPEGRELALADNRTSEMNLNWDKDILKNVFSPEVLEKYSMSLEKKKVEVNT
                     QRYPIVPKFDESYHAIIIIAKSETQLSFLKTALSLETEKSYKNDSTGTGYVIDAENFK
                     NKWEAK"
     gene            complement(1596..1991)
                     /locus_tag="PKX59_11850"
     CDS             complement(1596..1991)
                     /locus_tag="PKX59_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91590.1"
                     /translation="MKKSLVQILCFLLLSVTLTSVSFAQDESPGKVSTYATYQPKITV
                     HSVEVCNEVMEYSFDCPESYSFTPLQEPVAKIFTGSMELSNADHAKAQCKADSKVGWQ
                     RLILNSSINKVLLADIANSGTGTSYHYRC"
     gene            complement(2036..>2299)
                     /locus_tag="PKX59_11855"
     CDS             complement(2036..>2299)
                     /locus_tag="PKX59_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91591.1"
                     /translation="HKECKIQLNIPSNFDANQYFDKEGNLTANGAKSQTIVMLSGISA
                     NIHASHQAGVWDSAEHLRYIINELEKQFVQVANVDVTEYGNKV"
BASE COUNT          599 a          494 c          382 g          824 t
ORIGIN      
        1 cacaaagagt gtaaaattca actgaatata ccgtctaatt tcgacgctaa tcagtttgta
       61 gtcgtgtgtt atcatgtctt aatacggcaa tttaattact aattcataag gctttttagg
      121 tttcttatcc cacgttcctt ttttggtagc aactgcatca ccaaacattt ctttaagtaa
      181 aagaaaatca tttttctcca cttcttcagt acggacggat gccaggccac ctttattgcc
      241 gccatatcca tagaagtcaa acgtgaaacg ctcgtcctgc catatcattc tatgcttgta
      301 cgcattcatc aatgacagat aaatatcgtt attgcatttg atgtcattcc gcatcttaat
      361 cttggaacct gccaaccaac ccatagcttg gccgtacact aatcatttca gtttaatcgg
      421 tgaatgtcca tgatagtttt taggttgagc gtatctgttg aacgtgaata catacaaccc
      481 tgccatgtgg cacatattgc ctgtgaactg aattaaatcg tatgcagttt tgctatccac
      541 tttatcctgc tccttatttt cagagtacat tctacgcatt cctttgcaat catcatctaa
      601 cataaacaca tggccgaact tttcgtatat ccaattacgt tttgctccta atccaacaag
      661 tggaggatgc gtaactattt ctgtgtccgg gcacgatact cggtagaatt tttcctgttc
      721 ttcaggtaca catagtatgc agttatcaac caccttatgt gtggtaatgg tatcgtatct
      781 acctgcagtc ggtattacta ctttgatgtc tatttcgcct cccatttgtt tttgaaattt
      841 tctgcatcta ttacataacc ggtgcctgta ctgtcattct tgtaagactt ctcggtttct
      901 aaactcaatg cggtttttaa aaatgataac tgtgtttcag atttcgcaat aataatgatc
      961 gcatgataac tctcatcgaa tttcggaact atcgggtacc gttgagtatt cacctccact
     1021 tttttctttt cgagtgacat actgtatttc tccaatactt caggactgaa tacgtttttc
     1081 aggatatcct tatcccaatt caaattcatt tcagatgtac ggttgtcagc caatgccaat
     1141 tccctgccct ccggagtatc taagtcaata tccatacgct tcactgcaac cagggtattg
     1201 ccggtagttt ctacaaccac cacgtcgtca tatccctgct cctctgcatt tgatgttgtc
     1261 ttgtttcctg caatgatttg attgttctta tctatgagta ttgaacggcc taaaccattg
     1321 ctctcaatag atttttttat cattgcctta ccgttgtcag tacctttgtt tgcattcttt
     1381 gcatcgtgca ccaaatctga aatcttcatt ttttttaact gcaaatcttc ctgctctgca
     1441 ggaggagttt gctctttttt gttgttgccg ttattctttg ccatgttggt tttatttctg
     1501 taaaaataat tattttatac aactatacaa aataaaaaag atgcaacttt cgtcgcatct
     1561 tctaaatgtt tttaactttg ttttttttgg ctcactcaac accggtaatg gtaagacgtt
     1621 cctgttccac tattggctat gtcggcaagt aatactttat ttattgagga gtttagaatt
     1681 aatctttgcc acccgacctt actatcagct ttacattgtg cttttgcgtg atctgcgtta
     1741 cttagttcca tactgccggt aaaaatcttt gccaccggct cctgcaatgg agtgaatgaa
     1801 taggattcgg gacagtcgaa actgtattcc ataacctcat tacaaacttc tacggaatgc
     1861 accgtaattt ttggctgata agttgcatag gtactcacct ttccgggcga ttcatcctgt
     1921 gcaaaactta cacttgtgag tgttactgat aacagtaaga aacacaagat ttgtactaaa
     1981 gatttcttca taataattcc tgttttgagg atttaaaatt gattagaata taaatttaca
     2041 ctttatttcc atattctgtt acatctacgt tcgcaacctg cacaaattgt ttttccaatt
     2101 cgttaatgat gtaacgtaag tgctctgcac tatcccaaac accggcttga tgtgatgcat
     2161 gaatatttgc ggagatacct gatagcatta caatagtctg tgattttgca ccgttggcag
     2221 tcagattacc ctccttatcg aaatactgat tagcgtcgaa attagacggt atattcagtt
     2281 gaattttaca ctctttgtg
//
LOCUS       DAOOOI010000202         2179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_7813_length_2179_cov_4.787665, whole genome shotgun sequence.
ACCESSION   DAOOOI010000202 DAOOOI010000000
VERSION     DAOOOI010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7813_length_2179_cov_4.787665"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>443)
                     /locus_tag="PKX59_11860"
     CDS             complement(<1..>443)
                     /locus_tag="PKX59_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014798553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOZ91592.1"
                     /translation="SIAAYCGSLGVALATMVANLSASKKGWEERWNEFSDWADKGQKL
                     KKQLLHLVDEDTAAFNKIMDAFKLPKTTDQEKADRKQAVAEATRYAIEVPLSVMKISL
                     QSMELIQSMATNGNPNSVSDAGVGAMCARTAVLGAGLNVRINAAS"
     assembly_gap    444..463
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<464..1579)
                     /gene="ftcD"
                     /locus_tag="PKX59_11865"
     CDS             complement(<464..1579)
                     /gene="ftcD"
                     /locus_tag="PKX59_11865"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOZ91593.1"
                     /translation="MKQLIECVPNFSEGNDLTIIKQITDSIESVEGIKLLNVDPGKAT
                     NRTVVTFVGEPAAVVEAAFKGIAKAAELIDMSKHKGEHPRMGATDVCPFIPVANITMQ
                     ETAEWSKKLAARVGNKLHIPVYLYEEAQEDKKRSNLSIIRAGEYEGFFKKIKQAEWKP
                     DFGPAELPANSGATVIGARDFLIAYNVNLNTKSVKRANSVAFDIREAGRVKTDSNGKK
                     MLDDVGNEIRIPGKLKGVKAIGWYIEEYGIAQVSINITKFRETPLHLVFEESTRSAYE
                     RGYRATGSELVGLVPLQAMLDAGKYFLNKQRLSSGVSDAELIHIAVKSMGLDELSPFT
                     PEKRIIEYVMESESKPALANMRLNAFVDETASESPAPG"
     gene            complement(1728..>2179)
                     /locus_tag="PKX59_11870"
     CDS             complement(1728..>2179)
                     /locus_tag="PKX59_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HOZ91594.1"
                     /translation="FRYAYEQKFLSKYAIVGLHESYNAGNVLQFMMERPENFSLSFFE
                     DIFIRENISFTQAVDKAIEHVKDNFCGIEIDMDTIQNKTSSGISTIQARQYINRVASK
                     LNACYLHIAEAAPVLSHIKTDNKTGKLIGYLIADYIKARNTFKSQKN"
BASE COUNT          633 a          466 c          344 g          716 t
ORIGIN      
        1 atgatgcagc attaatacgc acattaagtc ctgcgcctaa aacagcagtt cttgcacaca
       61 ttgctcctac acctgcatct gaaacagaat taggattgcc gtttgttgcc attgactgaa
      121 tgagttccat tgactgtagt gaaattttca ttaccgataa tggtacttca attgcatagc
      181 gtgtggcttc agcaacagct tgttttctgt ctgctttctc ctgatctgta gtttttggta
      241 acttaaatgc atccattatt ttgttgaatg ctgctgtatc ttcatcaact aaatgaagta
      301 attgtttttt aagtttctga cctttatctg cccaatcaga aaattcattc catcgttctt
      361 cccatccttt tttagatgca gaaagattgg caaccattgt tgcaagtgca acacctaatg
      421 aaccgcaata agcagctatt gatnnnnnnn nnnnnnnnnn nnnacccgga gcaggtgatt
      481 cgctggcagt ttcatcaaca aaagcattca accgcatatt cgccagagca ggtttacttt
      541 cactctccat cacatattca ataattcttt tttcaggagt gaatggcgac aactcatcaa
      601 gccccattga ttttactgca atatgaatta actcagcatc actgacaccc gatgacaaac
      661 gctgcttgtt cagaaaatat tttcctgcat cgagcatggc ctgcaatgga accaagccca
      721 ccaactcact ccccgtagct cgatagccac gctcataggc acttcgtgtg ctctcttcaa
      781 aaacaagatg caagggagtt tctctgaact ttgtaatatt aatcgagacc tgtgcaatac
      841 catattcttc aatataccat ccaatcgctt taacaccttt aagcttaccc ggtattctga
      901 tttcattacc aacatcatct aacatttttt ttccattgct atcagttttc acacgacctg
      961 cttcacgaat atcgaaagca acagagtttg cacgtttaac agatttggta ttcagattta
     1021 cattataggc aattaaaaaa tctcgagcac caataacggt agcacccgaa tttgcaggca
     1081 attctgcagg accaaaatca ggtttccatt ctgcttgttt aatcttttta aagaagcctt
     1141 catattcacc agctctgatg attgaaagat tacttcgttt tttatcttct tgtgcctctt
     1201 catacaaata aaccggaata tgaagtttat tacctacacg tgcagctaat ttttttgacc
     1261 actctgctgt ttcttgcatt gtaatattgg ctacaggaat aaatggacaa acatcagtgg
     1321 ctcccattcg cggatgctca cctttgtgtt tgctcatgtc aataagttct gctgcttttg
     1381 ctatgccttt aaatgctgct tcaactactg ctgcaggttc acctacaaaa gttacaacag
     1441 ttcggttcgt tgctttaccc ggatcaacat tcaacaattt tattccttct accgattcaa
     1501 tgctatcggt aatttgtttg ataatagtta aatcattccc ttcactgaaa ttgggaacac
     1561 attcaatcaa ctgcttcatt taaatttgtt ttaatctttt taaaaaatat gtttgctaag
     1621 atcatcaaaa ttactctgaa aacatattcg ttaacgaaaa gcataaaaag ttttaaacga
     1681 aattttatta attgtaccct gagcaaatgt cattgggtat aatcagccta attcttttgg
     1741 cttttaaacg tattgcgtgc tttgatataa tctgcaataa gatagccgat taacttacct
     1801 gttttattgt cagtcttaat gtgtgaaagc acgggagcag cttcggcaat atgtagataa
     1861 caagcattta actttgaggc tacgcgatta atgtactgcc ttgcctgaat ggtactgatg
     1921 ccacttgaag ttttattctg aatggtatcc atatcaattt caataccgca gaagttgtcc
     1981 ttaacatgtt caatggcttt atcaactgcc tgagtaaatg aaatattttc tctgataaaa
     2041 atatcttcaa aaaaagacag tgaaaaattt tccggtctct ccatcataaa ctgaagcaca
     2101 ttacctgcat tatagctttc atgcaaaccg acaatggcat acttactgag aaacttttgc
     2161 tcataagcat atctaaatc
//
LOCUS       DAOOOI010000203         2165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_7872_length_2165_cov_10.169668, whole genome shotgun sequence.
ACCESSION   DAOOOI010000203 DAOOOI010000000
VERSION     DAOOOI010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7872_length_2165_cov_10.169668"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PKX59_11875"
     CDS             <1..376
                     /locus_tag="PKX59_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOZ91595.1"
                     /translation="KETFRYAPGKWMLKEVIGHLCDTERILTYRALCFARNDKTSFPG
                     FDENEYVANSNFKNRTMQSIGIEYLIVREASLALFNSLDETDFDRIGMANQTNTSARH
                     MLYFCIVHQRHHFNIIRERYYK"
     gene            complement(384..1541)
                     /locus_tag="PKX59_11880"
     CDS             complement(384..1541)
                     /locus_tag="PKX59_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOZ91596.1"
                     /translation="MNIYTQKQQWKLLLLASAILIGLLSLWYTNNLVKKLSEEERKKV
                     QLWAEASKQLANISVEAGDISFSLMVLQDNNTVPAILTDESGNILSHINLDTAKVNDS
                     QYVQEMLLQMKKRHEPIEISFANNQKNYIYYEDSGLLYQLRYYPYFQLAIIALFLLVS
                     YLAFSTSRKAEQNQVWVGMAKETAHQLGTPLSSLMAWIEYFKSTSQDQNHVAELEKDV
                     ARLNTITERFSKIGSAPVLRKEDVYMVIENAISYIRSRTSSKVIFSLNTAQPHQVLAP
                     LNIPLFEWVLENLFKNAVDAMDGEGKIDVMITDQLQFVYIDITDSGKGIPKSKFKTVF
                     KPGYTSKTRGWGLGLSLSKRIVEEYHNGHIFVKSSEPNKGTTFRIVLRKQV"
     gene            1723..>2165
                     /locus_tag="PKX59_11885"
     CDS             1723..>2165
                     /locus_tag="PKX59_11885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOZ91597.1"
                     /translation="MLNEQIIQGCKDKQGKAQHQLYELVAGRMMGVCLRYAKDDEEAK
                     DLLQEGFIKVFQQIDAFRGEGSFEGWIKKIFINTALESIRKRKLLTQPLEDEEDASDN
                     FLIQENKLEAKELLQMIRKLPDGYRVVFNLYAIEGFNHREIAQQL"
BASE COUNT          691 a          385 c          423 g          666 t
ORIGIN      
        1 caaagagacc tttagatacg caccgggtaa gtggatgctt aaagaagtga ttgggcactt
       61 gtgtgatacg gaacgaattt taacctaccg tgcattatgt tttgcaagaa acgacaaaac
      121 ttcatttcct ggttttgatg aaaacgaata tgtggcaaac agcaatttta aaaacaggac
      181 tatgcaaagt ataggtattg agtacctgat tgtgcgcgaa gcttcattag cattatttaa
      241 ttcgcttgat gaaactgatt ttgatagaat aggaatggca aatcaaacaa atacttcagc
      301 cagacacatg ctgtatttct gcattgttca tcaacgccat cattttaata ttatccgcga
      361 aaggtattac aaataagcta agattacact tgtttacgga gtacaatacg gaaagtagtt
      421 cctttatttg gttcagagct ttttacaaaa atgtgtccgt tatgatactc ttcaacaatt
      481 cttttactca acgaaagccc taatccccaa ccacgggttt tggatgtgta accaggttta
      541 aaaacggttt tgaattttga tttgggaata cctttgccac tatcggtaat gtcaatataa
      601 acaaactgca gttgatcggt aatcataaca tcaatttttc cttcgccatc catggcatca
      661 acagcatttt tgaaaagatt ttcaagtacc cattcaaata atggaatgtt taatggagca
      721 agcacctgat gtggttgtgc tgtgttcaga ctaaaaatta ccttactcga tgtgcgtgaa
      781 cgtatgtagg aaatagcgtt ttctataacc atgtaaacat cttctttacg gagtaccggt
      841 gcagatccga tttttgaaaa acgctcggtt atggtattca atcttgcaac atctttttca
      901 agttcagcca catgattctg atcctgtgac gttgacttaa aatattctat ccaagccatg
      961 aggctgctta atggtgttcc cagctgatgt gcagtttctt ttgccatgcc cacccaaacc
     1021 tgattttgtt cggctttgcg cgaagtgcta aaagcaagat aactcaccaa taaaaatagg
     1081 gcaataatgg ccaattgaaa ataagggtag tagcgtaatt gatataataa accggaatct
     1141 tcgtaataaa tatagttttt ctggttgttg gcaaaagaga tttcaatcgg ctcatgtcgc
     1201 tttttcattt gcaacaacat ttcctgaaca tattgagagt cattgacttt tgcagtatcg
     1261 agattgatgt gcgataaaat atttccactt tcatcagtta aaatagccgg cacagtattg
     1321 ttatcctgca aaaccatgag agaaaaactg atgtcgcctg cttcgacaga gatattggca
     1381 agttgtttgg atgcttcggc ccaaagttga acttttttac gttcttcttc gcttaacttt
     1441 tttactaaat tattggtgta ccacaacgac aatagcccta tgagtatggc tgaggccaaa
     1501 agcaaaagct tccattgttg tttttgtgtg taaatattca cccctttaaa ataaatcgta
     1561 cttacagttc aggtaaaaat ctatctatat tcggatgaat taattggatt acaggtgcaa
     1621 taatacttct tttctttcaa aaacacagaa attcttttta caaaaattaa aaccgatgca
     1681 acccttaccc ctacttttgc atctttaaag ataatagcag taatgctcaa tgaacaaatc
     1741 atacaaggtt gtaaagacaa acaaggcaaa gcacaacatc agctttacga acttgtggca
     1801 ggacgaatga tgggagtttg ccttcgttat gccaaggatg atgaagaggc caaagacttg
     1861 ttgcaggaag ggttcattaa agtgtttcag cagattgatg cctttagggg cgagggttct
     1921 tttgaaggtt ggattaaaaa aatctttatc aacacagcgt tagagagcat aaggaaaaga
     1981 aaattgctta cgcaaccgct ggaagatgaa gaagatgcaa gtgacaattt tctgattcag
     2041 gaaaataaat tagaagccaa ggaattgctt cagatgatca gaaagttgcc cgatggttac
     2101 agagtggttt ttaacctcta tgctattgaa gggtttaatc acagagaaat tgcacagcag
     2161 ttgaa
//
LOCUS       DAOOOI010000204         2127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_8044_length_2127_cov_9.416506, whole genome shotgun sequence.
ACCESSION   DAOOOI010000204 DAOOOI010000000
VERSION     DAOOOI010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8044_length_2127_cov_9.416506"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /locus_tag="PKX59_11890"
     CDS             <1..1522
                     /locus_tag="PKX59_11890"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOZ91598.1"
                     /translation="YMSESSRDITSLYMDDGYLFFNIDPVEVSAENDSIDLEMRIYEG
                     KQARINHVTVKGNTKTNDRVILREIRTKPGQLFSRSDIIRTQRELSQLGYFNPEKMGV
                     NPKPNPVDGTVDIEYEVEERPNDQVELSGGWGANQVVGTLGVTFNNFSARNILNTKAW
                     RPLPAGDGQRLSIRATTNGIYYQSYNASFTEPWLGGKKPNALTVSIYHSIQTNGLKKS
                     NDNRQAIFISGATVGLQKRLKWPDDYFTLYSGINYGLYTLKNYRSTFLFTDGTSNNIS
                     IEENLTRSSVDQPIYPRKGSEISFSVQFTPPYSAFTNKDYSKLDTKEKYKWIEYHKWK
                     FSIGWYTKLLGNLVMYNKMQFGFLGFYNKGIGQSPFERFYLGGDGLSGYALDGREIIA
                     LRGYENSALTPTDNIGNDVGGTIYDKFVTELRYPVSLNPSATIYVLGFFEGGNAWSTF
                     REFAPFSLQRSSGVGLRVFLPMFGLLGLDYGWRLDPQRDDPRTGVGKFSFVIGQQF"
     gene            1624..>2127
                     /locus_tag="PKX59_11895"
     CDS             1624..>2127
                     /locus_tag="PKX59_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOZ91599.1"
                     /translation="MKKIIIALALTVASSISVMAQKYAYVNSQYILDNIPEYKSAMQQ
                     LDQISVNWQKDIEAKYAVIDKLYKDYQAEQILLTEDMKRKREAEITAKEKEVKELQKS
                     KFGYEGDLFKKKQELVKPIQDKIYNAVKKMAVDQSYAVIFDKSSDLTMLYTNPKYDKS
                     DEILNAMG"
BASE COUNT          705 a          402 c          424 g          596 t
ORIGIN      
        1 atacatgagt gagtcaagcc gcgacattac ttcgctttat atggacgatg gatacctgtt
       61 tttcaatatt gatcctgttg aagtatctgc agaaaacgat tcaatagatt tggaaatgcg
      121 tatttacgaa ggtaagcagg caaggataaa tcatgttacc gttaaaggaa atacaaaaac
      181 caacgacaga gtaattttac gagagatacg tacaaagccc gggcaacttt tcagcagaag
      241 tgacatcatc agaacgcagc gcgaattgtc gcagttaggc tatttcaatc ccgaaaaaat
      301 gggagtaaac ccaaagccca atcctgttga tggcactgtt gacatagaat atgaagtaga
      361 agaaagacca aacgatcagg tagaactttc cggaggatgg ggagctaatc aagttgtggg
      421 tactttaggt gttacgttca acaatttttc tgcaagaaat attttaaata ccaaggcatg
      481 gcggccgctg ccagccggtg atgggcaaag attaagcatt cgtgctacta caaacggtat
      541 ttactatcag tcctataatg cttcgtttac agaaccctgg cttggtggca aaaaaccaaa
      601 tgcactcaca gtttcaatat atcactccat tcaaaccaat ggtttaaaaa aatctaatga
      661 caacagacag gcaattttta tttctggtgc aactgttggt ttacaaaaac gacttaagtg
      721 gcctgacgat tattttacac tctactcagg aataaattat gggctttata cacttaaaaa
      781 ttatcgctcc acatttttat ttaccgatgg aacttcgaac aacatttcta tagaagaaaa
      841 tcttacacgc agcagtgtag atcaaccaat ctatccgcgc aaaggatcag agatttcttt
      901 ttctgttcag tttactccac cctactctgc atttacaaat aaggattatt caaaattaga
      961 cacaaaagaa aaatacaagt ggatagaata tcacaaatgg aaattcagca ttggatggta
     1021 caccaaactt cttggtaatc ttgtaatgta taataaaatg cagtttggct tcctgggctt
     1081 ttataataaa ggcatcgggc agtcgccatt cgagcgtttt tatcttggtg gcgatggtct
     1141 ttccggctat gctcttgatg gtagagaaat tattgcatta cgtggttatg aaaatagcgc
     1201 actcacgcca acagataata tcggtaacga tgtaggtggt actatctacg ataaatttgt
     1261 aactgaatta cgctatcctg tatcgctcaa tccaagtgca acaatttatg tattaggatt
     1321 ctttgaaggt ggcaatgcat ggtcaacatt cagagagttt gcacccttta gtttacaacg
     1381 ttcatcaggt gtaggccttc gtgtatttct tccaatgttc ggcttactcg gtcttgacta
     1441 tggttggcgt ctcgacccgc aacgtgacga cccacgtaca ggtgtaggca agttctcatt
     1501 tgttatcgga cagcagtttt aatcctattc atccgttgtt caccgcaatt ggtgttagtg
     1561 gtacgatatt tagtataagg ttaagctaaa atcatatctt tgcacacatt aattaaacgt
     1621 aaaatgaaaa agatcatcat cgcattagca ctgacagttg cctcttccat ttcagtaatg
     1681 gctcagaagt atgcatacgt taactctcag tatattttag acaatatccc tgagtacaaa
     1741 tcagccatgc agcaattgga tcagatttct gtaaactggc aaaaagacat tgaagccaaa
     1801 tatgcagtca ttgacaaact gtataaagac tatcaggcag agcagatact gcttacagaa
     1861 gacatgaagc gcaagcgcga agcagaaatc actgcaaaag aaaaagaagt aaaagagctt
     1921 cagaaatcaa agtttggata cgaaggcgat ttatttaaga aaaaacaaga gttagtaaag
     1981 cctatacagg ataaaattta taatgctgta aagaaaatgg cagtagatca gagctatgcc
     2041 gtaatcttcg acaaatcgag cgaccttact atgctttaca ccaatcctaa gtacgacaag
     2101 agcgatgaaa ttttaaatgc aatgggc
//
LOCUS       DAOOOI010000205         2107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_8127_length_2107_cov_15.822125, whole genome shotgun sequence.
ACCESSION   DAOOOI010000205 DAOOOI010000000
VERSION     DAOOOI010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8127_length_2107_cov_15.822125"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..1164
                     /locus_tag="PKX59_11900"
     CDS             226..1164
                     /locus_tag="PKX59_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91600.1"
                     /translation="MKKQLFLITSAVVLTTFFYSCKKEDQQALDSKTKQFNDDSNNYK
                     AESDQADNDINNTINEIPAFGRGAGVVSSPLCGVTIDSSQLSQKILFFNFDGITPCFS
                     PSRTRSGQIKVQLTTGTNWHLAGSVLTITYNNFKITRLSDNKSITFNGVKTLTNVNGH
                     NWLTFLAGTSSFKYQSRAFNINVAYDDGSTATWNQANITEWLYVPSGNHGAYLIFTAN
                     GDSTVNNYSNSSMWGTNRYGQAFTTNYTTQIISNTYCGLWRPISGELIHHVNATDFAL
                     TLGVDQSGNASTLDCAYGFKVSWTSNGNSNSVVLSY"
     gene            1241..1996
                     /locus_tag="PKX59_11905"
     CDS             1241..1996
                     /locus_tag="PKX59_11905"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOZ91601.1"
                     /translation="MALQEEMEKQGLWLFRYRSTLPLLLLAGGLSYYLWSKMHHIRPF
                     PENSRSEEWFLYVCMAVSFLGLLMRIYIVGHTPANTSGRNTAEGQVADTINQTGMYSM
                     VRHPLYVGNFIMWLGIALFTASIWFVLLFIAVYWLYYERIMYAEEQFMRKKFGNAYTD
                     WAEKTPAFIPEFSNFKKPSLPFSWKKVLKKEKNGFAAVFIVFLLFYAAGQLITNNAIQ
                     NNFLVYGTVFSILFYIVIKILRKQTTLLNETGR"
BASE COUNT          674 a          359 c          370 g          704 t
ORIGIN      
        1 tacattttat tggcagcata gcatttcatt ttcgtaatat tttagagcaa attgcatcgg
       61 aaaagaaatt tattgtaggc aaaaccatta tacgacctgt tgatgacttg ttggtttatt
      121 tcacacaaga agaaaaataa acaatattgt atataatgtg acgttttttt aaaaaacaaa
      181 cttctgatgc ttaaaatgat tacttttatt ctaaattctg taaaaatgaa aaagcaactc
      241 tttttaatta catcagcagt ggtacttacc acattctttt actcctgcaa aaaggaagac
      301 cagcaagcat tagattcaaa gacaaaacag tttaatgatg attctaataa ttacaaagca
      361 gaatctgatc aggctgacaa tgacatcaac aacaccatta acgaaatccc tgcatttggc
      421 agaggtgctg gtgttgtatc aagcccgctt tgtggtgtaa caattgattc atctcagctt
      481 tcacagaaaa ttctgttctt taattttgat ggtatcacac cttgtttcag tccgtcacgc
      541 acacgatcag gtcaaattaa agttcagtta acaacaggta caaactggca tctggctggg
      601 tctgttctta ccattactta caacaatttt aaaataaccc gactcagcga taataaatcc
      661 atcaccttta atggtgttaa aacactgaca aatgttaacg gacacaactg gctaactttt
      721 ctggccggta caagcagttt taaataccaa agccgtgctt ttaatattaa tgtggcttat
      781 gatgatggct caactgccac ttggaatcag gcaaacatta ctgaatggtt gtatgttcca
      841 tcaggaaatc atggtgctta tcttattttt actgccaatg gcgattctac agtaaataac
      901 tattcaaaca gcagcatgtg gggaacaaac agatatggtc aggcttttac aaccaattat
      961 actacacaaa taatttcaaa cacctattgt ggtttgtggc ggcctatttc aggtgagttg
     1021 attcatcatg taaatgctac agattttgca ctcacgttgg gagttgatca aagtggaaat
     1081 gcaagcacac tcgactgtgc ttatggattt aaagtatcat ggacaagtaa tggaaattct
     1141 aattcagtag tccttagcta ttaacactat tttgttttaa aatgaaaaca gaatcttatg
     1201 gtaaggttct gtttttattt ttgtgccata ttcaaacagt atggccttac aagaagagat
     1261 ggaaaaacaa gggctatggt tattcaggta ccgtagcacc ctacccttac tacttttagc
     1321 aggaggcttg tcttattatt tatggagtaa gatgcatcac attagaccat ttccggaaaa
     1381 ttcaaggtct gaagaatggt ttttatacgt ttgtatggct gtatcgtttt taggcttatt
     1441 gatgagaatt tatattgtag gacacactcc ggcaaacact tctggtcgca atacagctga
     1501 aggtcaggtt gctgatacca ttaatcaaac aggaatgtat tcgatggttc gtcatccatt
     1561 gtatgttggt aattttatca tgtggcttgg catagcattg tttacagcaa gtatttggtt
     1621 tgtcttattg tttattgctg tttattggct gtactatgag cgtataatgt atgcagaaga
     1681 acaatttatg cgcaagaagt tcggcaatgc ctacaccgat tgggcagaga aaacgcctgc
     1741 ctttattcct gaattttcaa attttaaaaa accatcactt ccatttagtt ggaaaaaggt
     1801 tctaaaaaaa gaaaagaatg gatttgctgc ggttttcatt gtctttttac tcttttatgc
     1861 agctggtcaa ttaataacca acaatgctat tcaaaacaat tttttagttt acggaactgt
     1921 tttcagtatc ttattttata tagttattaa aatcctcaga aagcaaacta cattattgaa
     1981 cgaaaccggt cgttagaaaa attatttttt agatagcttt ttataaagct ccaaataaca
     2041 attagctgtt tgttcaatag aaatcagttt ttctccataa ggatattctt tctttattaa
     2101 ataacta
//
LOCUS       DAOOOI010000206         2106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_8129_length_2106_cov_29.203315, whole genome shotgun sequence.
ACCESSION   DAOOOI010000206 DAOOOI010000000
VERSION     DAOOOI010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8129_length_2106_cov_29.203315"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PKX59_11910"
     CDS             complement(<1..357)
                     /locus_tag="PKX59_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91602.1"
                     /translation="MKKLFVLALLVVSSFSINAQKLKEGTAHYKIDYNIESDEGGMKG
                     MLPTDANLSFKGDMFMMETTGGMFDQKTISDSKKQESTMLMDMMGNKVAVKVTKADID
                     KEKNKGTKPKVEITNET"
     gene            complement(419..1420)
                     /locus_tag="PKX59_11915"
     CDS             complement(419..1420)
                     /locus_tag="PKX59_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ91603.1"
                     /translation="MKETIYERAYHQQSLKDKAFLITGGSGFIGSNIVEYLLVHGAGK
                     VRVLDNLANGYKKNIEPFLNHPSFEFFEGDITNADTCLKAMEGIQFVSHQAALGSVPR
                     SLANPLATHHANATGFLNMLWAARQMNIERIVYASSSSVYGDSPELPKREHITGNPLS
                     PYAVSKMSNELYAYTFCMHYDMQIIGLRYFNVFGPNQNPDNPYAAAIPLFMKALLKDQ
                     PAYIFGDGEQSRDFTFVENAVQANIRALFSEHKDACNKVYNIAVGESISLNKLLHHLM
                     HITGKKIEPVYKEERPGDVKNSLADISLAKQYLGYNPLVKVKEGLALTVPWFKKNFC"
     gene            complement(1430..>2106)
                     /locus_tag="PKX59_11920"
     CDS             complement(1430..>2106)
                     /locus_tag="PKX59_11920"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOZ91604.1"
                     /translation="YHISTDEVYGSLGETGFFTENTSYDPRSPYSASKASSDHLVMAY
                     HHTYHLPVVLSNCSNNYGSHHFPEKLIPLAINNIKNMKPVPVYGKGENVRDWLYVEDH
                     ARAIDVIFHKGKEGEKYNVGGNNEWKNIDLIQLLCSVMDKKLNRKEGTSASLITFVTD
                     RPGHDLRYAIDSTKLQNELGWKPSLQFEEGLEKTVDWYLANDEWLQQVTSGEYMTYYQ
                     HQYDAR"
BASE COUNT          599 a          409 c          405 g          693 t
ORIGIN      
        1 tgtttcgtta gtgatttcaa ctttaggttt ggttcctttg tttttctctt tatcaatatc
       61 agccttagta actttaactg ccactttatt tcccatcata tccataagca tggttgactc
      121 ttgtttttta gagtcggaaa ttgttttctg atcaaacatg ccacctgttg tttccatcat
      181 aaacatatcg cccttaaacg ataagttggc atctgtaggc agcatgcctt tcatgcctcc
      241 ttcgtcagat tctatgttgt aatctatttt ataatgtgca gtaccttctt ttagtttttg
      301 tgcattgatg ctgaagctgc tcacaacaag taatgcaaga acaaataatt ttttcataat
      361 atttttgttt aaatcgttat taagcgaaag tagtaaagac aataagatgg aatactgttt
      421 agcagaaatt tttcttaaac cagggcaccg taagggcaag tccttccttg acttttacca
      481 agggattgta gccgagatat tgctttgcca gactaatatc tgccaaagaa ttttttacat
      541 cacccgggcg ttcttcttta taaacaggtt ctattttttt acctgtaatg tgcatcagat
      601 gatgtagcaa tttattaaga ctgatacttt cgcccacagc aatgttataa actttattgc
      661 aggcatcttt atgttcgctg aataaggctc tgatgtttgc ctgcacagca ttttcaacaa
      721 aagtaaaatc gcgcgattgt tcgccatcac caaaaatata ggcaggctga tctttgagca
      781 gcgctttcat aaaaagaggt atagccgctg cataaggatt gtcgggattt tgattgggac
      841 cgaaaacatt aaaataacgc agacctataa tctgcatatc gtaatgcatg cagaatgtgt
      901 aagcataaag ttcgttcgac attttggaaa cagcataagg cgataatgga ttgcctgtta
      961 tgtgttcgcg cttgggaagt tcggggctgt cgccataaac agatgatgaa ctggcataaa
     1021 caatacgctc aatattcatc tgacgtgctg cccacaacat atttaaaaat cctgttgcat
     1081 tggcatgatg tgttgctaaa ggattggcca acgaacgcgg cacagaacct aatgcagcct
     1141 gatgcgatac aaactgaatt ccttccattg cttttaggca tgtatcggca ttggtaatgt
     1201 cgccttcaaa aaattcaaat gatggatgat tcagaaaagg ttcaatattt tttttgtagc
     1261 cgtttgcaag attatcgagc acacgaactt ttcctgcacc atgcaccaac aagtactcaa
     1321 caatgttgct tccaataaaa ccggagcctc cggttataag gaatgctttg tcttttaaag
     1381 actgctgatg gtaggcgcgt tcatagatag tttctttcat cttttggggt tatcgtgcat
     1441 cgtactgatg ctggtaatag gtcatatatt caccggaagt tacctgttgc agccactcat
     1501 cattggcaag ataccagtca acagtttttt caagtccttc ttcaaattgc agtgatggtt
     1561 tccatcctaa ttcattctga agttttgtag aatctatagc atagcgtaaa tcatgtccgg
     1621 gtctgtctgt cacaaatgtt atgagtgatg ctgatgtacc ttcttttcga tttaattttt
     1681 tatccatcac agaacaaagc aattgtatca aatcaatatt tttccattca ttgttgccgc
     1741 caacattata tttctcacct tcttttcctt tgtgaaaaat tacatcaata gcacgtgcat
     1801 gatcttcaac atataaccag tcgcgcacat tttcgccttt accataaaca ggcacaggct
     1861 tcatattttt aatattgttg atggctaacg gtatcagctt ttcaggaaaa tgatgtgagc
     1921 catagttgtt ggagcagtta gaaagtacta cgggcaaatg ataggtgtga tgataggcca
     1981 ttaccaaatg atcggagctt gctttagatg cagaataagg gctgcgcgga tcgtaagagg
     2041 tgttttcagt aaagaaacct gtttcgccaa gagagccata cacttcatca gtactgatat
     2101 gataaa
//
LOCUS       DAOOOI010000207         2027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_8483_length_2027_cov_15.415822, whole genome shotgun sequence.
ACCESSION   DAOOOI010000207 DAOOOI010000000
VERSION     DAOOOI010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8483_length_2027_cov_15.415822"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKX59_11925"
     CDS             complement(<1..292)
                     /locus_tag="PKX59_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91605.1"
                     /translation="MLKKIVLTFLPLFISLLLNADTPLLKRVVCSYDDTTRLSFVSNA
                     NVFTERWDTLQQVKFWQEVIRQSADTALINVASTRCILHKTPFEDWKCQSESE"
     gene            complement(557..2005)
                     /locus_tag="PKX59_11930"
     CDS             complement(557..2005)
                     /locus_tag="PKX59_11930"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOZ91606.1"
                     /translation="MIRQIKKTAGILAIAAIGGFTSLAIYKQTEKPVLFGQQENTVPV
                     KRVSIAGNTEAAMFDFTEAAEKAVPAVVHVKTTYPSQPVSGNFFNPFNFWGAPGYGAP
                     QQPQQSAGSGVIISDDGYIVTNNHVVDNADKVDVTLDDKRTFTAKVIGKDPSTDLALL
                     KIDQTGLPYSKFANSDDIKVGQWVLAVGNPFNLTSTATAGIVSAKGRNIHILNDQQFP
                     IESFIQTDAAVNPGNSGGALVNLNGDLVGINAAIASNTGSYTGYSFAIPANIVRKVTK
                     DLLEYGKVQRGFIGVTLRDIDQQLAQNKGLKSMQGVYIDGVVSGGAGQAAGIKPGDVV
                     TKVGETTVNSASELQEQIGRFGPGDKVNLTLNRDGTEKNYQIVLRDNSGGERVERANN
                     NVNMMGAEFGEVTDKEKKQLGISNGVKVAGLQSGKLRSAGIREGFIITSIDNKKINSP
                     DDIEAALANKKGGVLIEGVYPNGMRAYYGFGL"
BASE COUNT          602 a          430 c          325 g          670 t
ORIGIN      
        1 tttctgattc tgactgacat ttccaatctt caaatggtgt tttatgaagt atgcatcgtg
       61 tagaggcaac gttaatcagt gccgtatcag cggactgtct gattacctcc tgccagaatt
      121 taacctgctg aagtgtgtcc catcgctctg taaaaacatt tgcattagaa acaaaactca
      181 accgggttgt atcatcataa ctacaaacaa ctctttttaa taaaggcgtg tctgcattta
      241 acagcaagct gatgaacagt ggtaaaaatg taagtactat tttttttaac atcaattgca
      301 tttatagtta tacggaaatt aacataacct gtttagactt tttttattgt tttcttgcat
      361 cttttgcaaa ggtaggctat ttaaaataga aatattaagc cactgcaagt taaacaaaat
      421 tcgttttcaa cacaagagta ttaactatta tttgaattgt gagagaaata ctgcaattta
      481 taagttgcat aaaatgcaaa gggagattca ttttcagaat cccccttcgc tataaaccaa
      541 acctatgaaa aactatctat aatccgaatc cataataagc tctcatgcca ttaggataaa
      601 caccttcaat cagcacacca ccttttttat tggctaatgc agcttcaatg tcgtcaggag
      661 aatttatttt cttattatca atgctggtaa ttataaaccc ttcgcgaata cctgcacttc
      721 tcaactttcc tgattgtaaa cctgcaacct taacaccatt gcttatgcct aattgttttt
      781 tctctttatc agtcacttct ccaaattcag cacccatcat gttgacattg ttattagcac
      841 gttcaacacg ttcaccacca ctgttgtcgc gcaatactat ctgataattt ttttctgtac
      901 catcacgatt caatgtaaga ttaactttat caccagggcc aaagcgtccg atttgttcct
      961 gaagctcact tgcagaatta actgtagtct cgccaacctt agttaccacg tcaccgggtt
     1021 ttattcctgc tgcttgtccg gcaccaccac tcacaacgcc atcaatataa acaccctgca
     1081 tagattttaa tcctttattc tgtgctaatt gctggtcaat atcgcgtaat gtaacaccaa
     1141 taaatcctcg ttgaacctta ccatattcca ataaatcttt agttaccttt cttacaatat
     1201 ttgcaggaat ggcaaaagaa tagccggtat atgaacctgt attggaagca atagcagcat
     1261 tgatacctac caaatcaccg ttcaggttta ctaaggcacc accactgttg ccgggattca
     1321 cagcagcatc tgtttgaata aatgattcaa tcggaaactg ctgatcattt aaaatatgaa
     1381 tgttacggcc ttttgctgaa acgatacctg cagtagcagt tgaagtaaga ttaaaagggt
     1441 taccaacagc caacacccat tgacctactt taatatcatc tgagtttgca aattttgaat
     1501 aaggcaaccc tgtctgatca attttaagca atgccaagtc tgttgaagga tcttttccta
     1561 taacctttgc agtgaaggtt cttttatcat ctaaagtaac atcaacttta tctgcattgt
     1621 caacaacgtg attgtttgtt actatatatc catcatcaga aataattaca ccggaacctg
     1681 ctgattgttg tggttgttgt ggcgcaccat agccgggtgc tccccaaaag ttaaatggat
     1741 taaaaaaatt accactcaca ggttgtgatg gataagttgt tttaacgtgt acaacagcag
     1801 gaacagcctt ctctgcagct tctgtaaagt caaacatggc agcttctgta ttgcctgcta
     1861 tacttaccct tttaacggga actgtgtttt cttgttgacc aaacaataca ggtttttctg
     1921 tttgcttata tatggccaat gaagtgaagc cgcctattgc tgctattgcc aatattccgg
     1981 ctgttttttt aatctgtctt atcattactt ataatcttgt atgtttt
//
LOCUS       DAOOOI010000208         2010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_8567_length_2010_cov_5.791816, whole genome shotgun sequence.
ACCESSION   DAOOOI010000208 DAOOOI010000000
VERSION     DAOOOI010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8567_length_2010_cov_5.791816"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1073
                     /locus_tag="PKX59_11935"
     CDS             90..1073
                     /locus_tag="PKX59_11935"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HOZ91607.1"
                     /translation="MKRVRLIIVTMSLFQIVSVKLNAQTVSDFENLTLPADSFFNGSS
                     QQGGTSFTSGNAIFPNFFDPSFQYWLSGWAYSNVADSSTAGFGNMYASASLTGYNGSG
                     IYALGQQNATITLSGNASGKVVNGFFVTNATYPYISMRDGDGFSKKFGGPSGNDPDYF
                     ILTIHNWYNGNLSSDSINFYLADFRSANNAQDYIINDWQWVDLTGLGNTDSLLFTLSS
                     SDVGSFGINTPLFYCIDNFTTADSPVGISEIKNNVVQVYPNPASSYISLTKNIDEIFI
                     TDVEGRVVMESLKLNAGDKISVSQLKDGLYFINAHYNNESTKFKFVKCSTH"
     gene            1058..>2010
                     /locus_tag="PKX59_11940"
     CDS             1058..>2010
                     /locus_tag="PKX59_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ91608.1"
                     /translation="MFDSLKTVLIALACLFSLTESNGQFAPPASQPGTTAIHKDSSII
                     VSWATQCSIVRGWQDISNHSLGVCTIGDSTSALGMADGLDVVSLGDGGMATLTFANPI
                     MNGSGWDFAVFENSFSETFLELAFVEVSSDGVNFFRFPSVSLTQDTVQVGSFGSIDAT
                     QIDNLAGKYSAFYGTPFDLDIMTGISGLDVNHITHVRVIDVVGSIDELYATYDSQFNK
                     INDPWPTPFPSSGFDLDAVGVIHQNTTSVNSIIENQFNLSYPNPFNKNNVIKLNLTKT
                     EDLSMTLYDISGKAIYQFLDGKINAGEHLFNLRNSLIGNGI"
BASE COUNT          654 a          358 c          379 g          619 t
ORIGIN      
        1 aactatgaag caattcgaaa ccgttcaatg cccttacgct catacaaagc aggaattatt
       61 attcagataa attcaaaatc aaaataaata tgaaaagagt tagactcatc attgtaacaa
      121 tgtcactatt ccaaatagtg tctgtaaaac tgaatgcaca aacggttagt gacttcgaaa
      181 acctgacttt accggcagat agcttcttta atggctcatc acagcaaggc ggaacaagtt
      241 tcacatcagg aaatgcaatt tttcctaact ttttcgaccc atcatttcaa tactggcttt
      301 cgggctgggc atacagtaat gttgcagaca gcagcactgc aggcttcgga aacatgtatg
      361 ccagcgcatc attgacaggg tataacggct caggcattta cgcccttggt cagcaaaatg
      421 caaccataac actttcaggc aatgcttcag ggaaagtagt taatggattt tttgttacca
      481 atgctactta tccatatatc agtatgagag atggagatgg tttttcaaaa aaattcggag
      541 ggccatcagg aaatgatcct gattatttta ttctaaccat acacaattgg tataatggaa
      601 atttaagtag tgatagtatt aatttttatc ttgctgactt tcgttctgca aataatgcgc
      661 aggattatat catcaacgat tggcaatggg tagatttaac tggacttggc aatacagaca
      721 gtttgctttt tacactttct tcaagcgatg ttggaagttt tggtatcaat acacctttat
      781 tctattgtat tgacaacttc accacagcag attcaccggt aggaatttca gaaattaaaa
      841 acaatgtagt acaagtatat ccaaatcctg catcttccta tatctcatta actaaaaata
      901 tagatgaaat ttttatcact gatgttgaag gtcgggttgt tatggaatca ctcaaactaa
      961 atgcaggaga taaaatatct gtttcacaac taaaagatgg attatatttt atcaatgcac
     1021 actacaacaa cgaatcaaca aaatttaaat ttgtaaaatg ttcgactcat taaaaactgt
     1081 attgattgca ttggcttgcc tgttttcatt aactgaaagt aatggccagt ttgcaccacc
     1141 tgccagtcag ccgggcacaa cagctattca taaagacagc agcattattg tttcgtgggc
     1201 aacacaatgt agtattgtca gaggatggca ggacatttct aatcacagtt taggtgtttg
     1261 cacaataggc gacagcacat ctgcattagg tatggctgat ggcctcgatg tagttagcct
     1321 tggtgatggc ggcatggcta cattaacatt tgcaaatcca atcatgaatg gtagtggatg
     1381 ggattttgca gtatttgaga acagctttag cgaaacattt ctggaacttg catttgttga
     1441 agtgagtagt gatggtgtaa acttttttcg ttttccttca gtgtctttaa cgcaagatac
     1501 tgtgcaggtt ggaagcttcg gaagtattga tgcaacacaa attgataatc tggcaggcaa
     1561 gtacagtgca ttttatggaa caccattcga cttagatata atgacaggaa tttcagggct
     1621 tgacgtaaat catatcacac atgttagagt aattgatgtg gtgggaagta ttgatgaatt
     1681 gtatgcaact tacgattcgc aattcaataa aattaatgac ccctggccta ctccatttcc
     1741 aagttccgga ttcgaccttg atgcagtagg agtcattcat caaaatacaa catcagtaaa
     1801 ttcgattata gaaaatcagt tcaatctctc ctaccccaat ccatttaata aaaacaatgt
     1861 gattaaatta aatcttacta agactgaaga cctaagtatg acattatatg acatttcagg
     1921 aaaagctatt tatcagtttt tagatggaaa aatcaatgcc ggtgaacatc ttttcaatct
     1981 tagaaactct ttaattggta atggaattta
//
LOCUS       DAOOOI010000209         2007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_8575_length_2007_cov_7.282275, whole genome shotgun sequence.
ACCESSION   DAOOOI010000209 DAOOOI010000000
VERSION     DAOOOI010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8575_length_2007_cov_7.282275"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..>2007)
                     /locus_tag="PKX59_11945"
     CDS             complement(61..>2007)
                     /locus_tag="PKX59_11945"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HOZ91609.1"
                     /translation="IQQVAPFGFGNTVPRWWNTNPISGAAGWFQYQVTIPNIPVVTVA
                     SQRFLIFVFTNRHTAASGGNIYIDDISINTYPKAQAYVSTKMSFQGTASVPQNSLNNL
                     IYGVEVVIDGGDDVTANNYYTVDSLNFSYIGSTNACGDAQNAKLWWTGGTDVFNGVAN
                     AIQVGATIPTLCATNYSFFPVNTFRLNNGKNYFWVTYDIKPVGTSTPGNCVDGEYNMI
                     RLTKGGIPMTGTPVPGTLAGCRPIDVAYCGGATPSYGVGTSWLGGSYTNNDYVAQVIL
                     NGEVGYPVINNALNCCGPNIAPWWGGPAPFSAHPPDYEKFPAVPGKTTVLMANGMTSY
                     TISLKVGTWYSSNYIAAFIDFNHDGVFTTATERIAMSPNMAANGTYTTTFTVPTTAYI
                     GTTTLRVREVYANSNIDACAYGTYGETEDYSVTIIPTCNSWPGLGYNKLWLGGFDNDW
                     ANPLNWCPANPPLITDNALIPGSSPHRPVIKEGTLAYAKKLRIQGNDTVTVNVYKTGR
                     LTVADSLIINDPATVLRVNSQFTDTARISVGNLLPTIPPYYPLNAAALKQRCQLVYTS
                     TELSSYGMITGDEIDSIFLLVSNFTAPANAYTNFSIRWYYTSGGFSYGGTAGTNALAA
                     FAIGTTPNLVYTNPSLSVPATGQY"
BASE COUNT          623 a          407 c          401 g          576 t
ORIGIN      
        1 acatatatct attaccaaag aatatccaag gttaacagga ataggtgtca acaaaccaaa
       61 ttaatactga cctgttgccg gaacacttag cgatggattg gtataaacta aattgggtgt
      121 ggttccaatt gcaaatgcag ccaatgcatt ggttcctgca gttccaccat aagaaaatcc
      181 tccactggta tagtaccatc tgatgctgaa gtttgtatat gcgttcgctg gagctgtaaa
      241 atttgacacc aataggaata tggaatcaat ttcatcaccg gtaatcatgc cataagaact
      301 taactcagtg gaagtataaa ctaactgaca acgctgtttt aaagctgctg cattcaacgg
      361 ataatatggt ggaatagtag gcaacaaatt acctactgaa atacgggcag tatcagtaaa
      421 ttgcgagtta actctaagta ctgttgcagg gtcgttaata attaatgagt cggcaactgt
      481 caggcggcct gttttataaa cattaactgt taccgtatca ttaccttgta ttcttaactt
      541 tttagcataa gctaaagtac cttctttaat tacaggacgg tgagggctgc tacctggtat
      601 aagtgcatta tcagttatca atggcggatt tgcaggacac cagtttagtg ggttagccca
      661 atcattatca aaaccaccaa gccacaattt attataaccc aagcccggcc atgagttaca
      721 tgtaggaatt atagtaacag aataatcttc tgtttcaccg taggttccat aagcacaagc
      781 atctatattt gagtttgcat aaacttcacg cacacgtaaa gttgtcgtac ctatataggc
      841 agttgttggc actgtgaaag tagttgtata ggtaccatta gctgccatat taggtgacat
      901 ggcaattctt tctgttgctg tagtaaatac accatcgtgg ttaaagtcaa taaaagctgc
      961 aatatagttt gaactatacc atgttcctac ttttaaacta atggtatagg aagtcatacc
     1021 atttgccatt aacacagtag tttttcccgg tacggcaggg aatttttcgt aatcgggcgg
     1081 gtgggcactg aaaggtgccg gaccacccca ccaaggtgca atattaggac cacaacagtt
     1141 taatgcatta ttgattacgg gatatccaac ctctccatta agtataacct gtgctacata
     1201 atcgttgttt gtataggaac caccaagcca tgatgtacct acaccgtatg atggtgttgc
     1261 accaccacaa taggccacgt caattggccg gcaacctgcc aaagttccgg gcacaggagt
     1321 tccggtcata ggtatgccac ctttagtaag acgaatcata ttgtactcac cgtcaacaca
     1381 attgcccggt gtggatgttc caacgggttt aatatcataa gttacccaaa aatagttttt
     1441 accattgttg agtctgaaag tgtttaccgg aaaaaaagaa tagtttgttg cacataaggt
     1501 aggtatagtg gcaccaacct gaatagcatt ggctacacca ttaaacacat ctgttccacc
     1561 tgtccaccac aatttagcat tttgtgcatc gccacaggca ttggtagagc caatgtagct
     1621 aaagtttaat gagtcaactg tgtagtagtt gttagcagtt acatcatcac cgccatcaat
     1681 taccacttca acaccataaa ttaaattatt caatgagttt tgaggtactg atgcagtacc
     1741 ctgaaaagac atttttgtag aaacatacgc ctgtgccttt gggtaggtat tgatagagat
     1801 atcgtcaata taaatatttc caccgctagc tgcagtatgt ctgttggtaa aaacaaaaat
     1861 cagaaatcgc tgtgaggcta cggtaacaac aggaatattt ggaattgtta cctgatattg
     1921 aaaccatcct gcagcaccac taattggatt agtattccac caacggggca ctgtatttcc
     1981 aaagccaaaa ggtgcaacct gttgaat
//
LOCUS       DAOOOI010000210         1946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_8875_length_1946_cov_6.575886, whole genome shotgun sequence.
ACCESSION   DAOOOI010000210 DAOOOI010000000
VERSION     DAOOOI010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8875_length_1946_cov_6.575886"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PKX59_11950"
     CDS             <1..504
                     /locus_tag="PKX59_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOZ91610.1"
                     /translation="GTGAGERFLIGSNAGRVVKNAPCPVLLIPPRVKFNGLKKLVYAT
                     DLSDDNLSKASELIPFAKAFNSELLFLYIDTTTLSKDEIDVKKITDLVRKKVKYPKQS
                     GYVCTDVSIQNGVSHFLKKFKADALVMYTRHRNLFKGLYKPSIAKSVSYNTKVPLLVV
                     HENDTLF"
     gene            655..>1946
                     /locus_tag="PKX59_11955"
     CDS             655..>1946
                     /locus_tag="PKX59_11955"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HOZ91611.1"
                     /translation="MQNLIKSKLAMLIIAVFLYGQSIAGNNCWKPVEEVSLSGMGKMN
                     RNTIPSTARYYELNMQQLQSLLQQAPNRGQQSNLIIDFPDAYGNMNAFRVYASPIMPE
                     MLSSKYPMIKTYCAQGINDPTATMRFSVTQFGLHCMTLSGTNPTNYIDTYTKDLNYYI
                     VYDKASLANNVRNFECLTDEDAELPSMRNGVAGINNNTDDQLLRTYRLALTCTAEYGN
                     IFATTPGSEKADIMAQMTITMNRVNGVYERDLAITMEFVPNNDTLIFYGNTSQDPWSN
                     EWNTKTAQVCDQYIGVANYDIGHNFNTTGGGNAGCIACVCLSSSQSGTHKGRGMTGSA
                     NPVGDPFDIDYVAHEMGHQYGGYHIMNTCSRSGSGNTEVEPCSGSSIMGYAGICSVNI
                     QAHSDDDFNYVNVRDISANIQNGSSTCAVTTPLTDNPPT"
BASE COUNT          639 a          374 c          380 g          553 t
ORIGIN      
        1 ggcaccggtg caggcgaacg gtttttaatt ggaagcaatg caggtcgtgt tgtaaaaaat
       61 gcaccatgtc ctgtacttct tattccaccg cgtgtaaaat tcaatggact aaaaaaactt
      121 gtttatgcaa ctgacctttc tgatgacaac ctgagcaaag catcagaact gattccattt
      181 gctaaggcgt ttaacagtga acttttattt ctttacattg atacaacaac tttatctaaa
      241 gatgaaatag atgttaaaaa aataacagat ctggttcgca aaaaggtaaa atatccaaaa
      301 caatcaggat atgtatgtac tgatgtttct attcagaatg gtgtgagtca tttccttaag
      361 aaattcaaag ctgacgcctt ggtgatgtac acccgtcatc gcaacttatt taaagggtta
      421 tataagccaa gtatagcaaa aagcgtgtcc tacaacacca aggttccttt gctggtagtg
      481 catgaaaacg acaccctttt ttagccttat aaggtactga gattactcat ttattaactg
      541 attgcaaact gtaattgtac ttgtttctgt tactgctata ttagcggaat aatcggtgca
      601 tgaggtcatt tcatacacca tttccaaatc aaacaaaaac aaatcaaatt acacatgcaa
      661 aacttaatta aaagcaaatt agccatgcta atcattgcag tatttctcta tgggcaatcc
      721 attgcaggga ataactgctg gaaaccggtt gaagaggttt ctttatcagg aatgggaaaa
      781 atgaatcgaa acaccattcc gtcaactgcc cgttattatg aattgaacat gcagcagttg
      841 caaagtttat tacagcaggc accaaatcgt ggtcagcagt caaatctgat tatagatttc
      901 cctgatgctt acggaaacat gaatgccttc agagtttatg catcaccaat aatgcctgaa
      961 atgctttcat ccaagtatcc gatgattaaa acatattgcg cacaaggtat aaatgaccca
     1021 acagcaacaa tgcgttttag tgttacacag tttggtctgc attgtatgac tttatcagga
     1081 acaaatccga caaattatat tgacacttat acaaaggatt tgaattatta cattgtttat
     1141 gacaaagctt ctttagccaa taacgtccgc aattttgaat gtctcactga tgaagatgca
     1201 gaactaccaa gtatgcgaaa tggtgttgca ggtataaata acaacactga tgatcagtta
     1261 ttgagaacct acagacttgc acttacctgc actgccgaat atggaaatat ttttgccaca
     1321 acaccgggaa gtgaaaaagc cgatatcatg gcgcaaatga caattaccat gaatcgtgta
     1381 aatggtgttt acgagcgcga ccttgcaatt actatggaat ttgtgcccaa taacgacaca
     1441 ttgatttttt atggaaacac ctctcaggat ccatggtcaa atgaatggaa tacaaagaca
     1501 gcacaggttt gcgatcagta cattggtgtt gccaactacg acattggaca taactttaac
     1561 accacaggcg gtggtaatgc aggttgtatt gcctgtgttt gtttaagctc ttcgcagagt
     1621 ggaacacata aaggtcgtgg catgacagga agtgcaaatc ctgttggcga tccattcgat
     1681 attgactatg ttgcacatga aatgggacat cagtatggtg gctatcacat catgaacaca
     1741 tgcagccgca gtggcagtgg aaacacagaa gtagaacctt gcagtggtag ctcaattatg
     1801 ggttatgcag gtatttgttc agtaaacata caagcacata gtgatgatga ttttaattat
     1861 gttaacgtaa gagatatctc tgcaaacatt cagaacggta gcagcacttg cgcagttaca
     1921 acaccattaa cagacaaccc tcctac
//
LOCUS       DAOOOI010000211         1894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_9122_length_1894_cov_27.506253, whole genome shotgun sequence.
ACCESSION   DAOOOI010000211 DAOOOI010000000
VERSION     DAOOOI010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9122_length_1894_cov_27.506253"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PKX59_11960"
     CDS             complement(<1..391)
                     /locus_tag="PKX59_11960"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOZ91612.1"
                     /translation="MTLTLGLLWTSCNEDRPLRTLPVYGMQDEQNKSNHTIGSFSFID
                     QNGDTVTDKDYSNKIYVTDFFFTTCRSICPVMTTQMQRVFEHYKSNPDVMFLSYSVNP
                     EYDTPAVLKEYADKHHADAKKWHFVTGN"
     gene            complement(451..729)
                     /locus_tag="PKX59_11965"
     CDS             complement(451..729)
                     /locus_tag="PKX59_11965"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HOZ91613.1"
                     /translation="MRKVIVALLILFTACQPKSSEPEQKTAPALEEKEFPAKPDIALA
                     SDIDPVCKMSVKEEFSDTAMYDGKIYGFCASACKEDFVKEPLTYLEEK"
     gene            827..1327
                     /locus_tag="PKX59_11970"
     CDS             827..1327
                     /locus_tag="PKX59_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOZ91614.1"
                     /translation="MKKQVYTALTILMVFGVIKQTNAQEKVTIYHPEKNAFEQIKQAE
                     ITAQKEGKHIFLMIGGNWCRWCKMFDTFSKTDLSVDSAFKANYVVEHINYSKENKNDS
                     LMQQLEFPQRFGFPVFVILDAHGKRLHTQSTGYLEKGEGYNKESVIEFLNQWSPAALN
                     PANYKK"
     gene            complement(1337..>1894)
                     /locus_tag="PKX59_11975"
     CDS             complement(1337..>1894)
                     /locus_tag="PKX59_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91615.1"
                     /translation="LFTQIWKTECGGFKNESQNYSQIALANIVIFERFLQSNKDSLWL
                     VQGEKMMANYKKSKTQGIAGINKLKQSLELYSDDLMDGTIPADFFISRMTSIQKELAA
                     YKIKKTNEEETNLSMTQYLALGKQLIRKRQLDDAIKILKLNIQHYPNCVTAYDSLSRI
                     YKIQGNKQMALFYNQKGASIKNPAY"
BASE COUNT          607 a          310 c          322 g          655 t
ORIGIN      
        1 tgttgccggt cacaaaatgc catttcttgg catctgcatg atgcttatct gcatattctt
       61 ttaaaacagc aggagtatca tattcaggat ttactgaata cgataaaaac ataacatcag
      121 gatttgattt gtaatgttca aatacacgct gcatctgtgt tgtcattaca ggacaaatac
      181 ttctgcatgt ggtgaagaag aaatcagtca catatatttt attggaatag tctttatctg
      241 taactgtatc tccattctga tctataaaag aaaaagatcc tattgtatga ttggatttat
      301 tttgttcatc ctgcataccg taaacaggta atgttcgtaa tgggcgatct tcattgcacg
      361 atgtccacaa caatcctaaa gtaagagtca ttaatattag aagccattcc aaagcaggaa
      421 ttgaccaaag tcttttacaa ctaatacaac tcatttttct tcaaggtagg tgagtggttc
      481 tttaacaaaa tcttctttac atgccgatgc acaaaaacca taaattttac catcatacat
      541 tgctgtatca gaaaattctt ctttaactga cattttacaa accggatcaa tgtccgatgc
      601 taatgcaatg tccggttttg caggaaattc tttttcttca agtgccggtg ctgttttttg
      661 ttcaggctct gaagacttag gttggcatgc cgtaaaaaga attaaaagtg ctacaataac
      721 tttcctcatt tttttgttta tttgaacttt acaaagttat taaatacaac ctttatcaca
      781 tgaaggattc aggtattatt ttaaacgaat caattgaaaa tttagtatga aaaaacaagt
      841 ttacactgcg ctgacgatac tcatggtttt tggagtgatt aagcagacta atgcacaaga
      901 aaaggttaca atttaccatc ctgaaaagaa tgcatttgaa cagataaagc aagcagaaat
      961 tactgcacag aaagaaggaa aacatatttt tctgatgatt ggaggtaatt ggtgtcggtg
     1021 gtgcaaaatg tttgatactt tcagtaagac agatttatca gttgattcag catttaaagc
     1081 taactatgtt gtggaacata ttaattacag taaggagaat aaaaatgatt cactgatgca
     1141 acaactggag tttccgcaac gttttgggtt tcctgttttt gttattcttg atgcccatgg
     1201 aaaaagattg catacacaat ctaccggcta tctagaaaaa ggagagggat ataataaaga
     1261 aagtgtaatt gagtttttaa atcaatggtc tcctgctgca ttaaatccag caaactataa
     1321 gaaataaatt caaattttaa taagccggat tttttataga ggcacctttt tgattgtaaa
     1381 ataaggccat ctgtttatta ccctgaattt tataaattcg gcttaatgag tcgtatgcag
     1441 ttacacaatt ggggtagtgc tgaatgttta atttcagaat tttaattgca tcatccaatt
     1501 ggcgtttgcg aataagttgt tttcctaatg ccagatattg tgtcatactc agattagttt
     1561 cctcttcatt cgttttttta attttatatg cagcaagttc tttctgaata cttgtcattc
     1621 gggaaataaa aaaatctgcc ggtattgttc cgtccattaa atcatcacta tagagttcaa
     1681 gactttgctt aagtttgttt attcctgcta tgccctgagt tttgcttttt ttataattag
     1741 ccatcatttt ttctccttgt actaaccaaa gtgaatcttt atttgactga agaaaacgct
     1801 caaaaatcac aatgttggca agtgcaatct gagaatagtt ttgcgattca tttttaaatc
     1861 caccacattc tgttttccaa atttgagtaa acaa
//
LOCUS       DAOOOI010000212         1882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_9186_length_1882_cov_5.461959, whole genome shotgun sequence.
ACCESSION   DAOOOI010000212 DAOOOI010000000
VERSION     DAOOOI010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9186_length_1882_cov_5.461959"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1047)
                     /gene="ftsY"
                     /locus_tag="PKX59_11980"
     CDS             complement(97..1047)
                     /gene="ftsY"
                     /locus_tag="PKX59_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOZ91616.1"
                     /translation="MAFFGLFSKKDKESLDSGLNKTRENFFTKITKAVIGKSTVDDEV
                     LDNVEEILVSSDVGVDTTLKIVKRIQDRVARDKYVGTDQLNTILKEEIAALLGENKSA
                     TVANLIDESKKPYIIMVVGVNGVGKTTTIGKLAYQFKQSGKNVLLGAADTFRAAAVDQ
                     LNIWAERVQVPIVSQGMNADPASVAFDTVKSGVAQNSDVVIIDTAGRLHNKVNLMNEL
                     TKIKRVMQKVVSDAPHEILLVLDASTGQNAIEQARQFTAATEVNALALTKLDGTAKGG
                     VVIGFSDEFKIPVKYIGVGEKMTDLQAFDRVSFVDSLFAK"
     gene            complement(1130..1282)
                     /locus_tag="PKX59_11985"
     CDS             complement(1130..1282)
                     /locus_tag="PKX59_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOZ91617.1"
                     /translation="MAKKVVATLKTGKGKEFSKVIKMVKGANGAYSFKEEIVHNDHVK
                     DFLKEK"
     gene            complement(1300..1482)
                     /gene="rpmG"
                     /locus_tag="PKX59_11990"
     CDS             complement(1300..1482)
                     /gene="rpmG"
                     /locus_tag="PKX59_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOZ91618.1"
                     /translation="MAKKGNRVQVIMECTEHKNSGMPGTSRYITTKNKKNTTERLELK
                     KYNPILKKVTLHKEIK"
     gene            complement(1495..1728)
                     /gene="rpmB"
                     /locus_tag="PKX59_11995"
     CDS             complement(1495..1728)
                     /gene="rpmB"
                     /locus_tag="PKX59_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOZ91619.1"
                     /translation="MSRICDLTGKTSIVGNNISFSNRKTKRRFNPNLQTKKFFIPETG
                     EWVTLKVSASAIRTINKKGISACINSLIKKGHI"
BASE COUNT          552 a          388 c          276 g          666 t
ORIGIN      
        1 tatcagtacg gataaggttg attataaccc caaccttgat gataatatga atgaccaaaa
       61 atggcaggag ttaaaagaaa ttaaatagca gtaatgctat tttgcaaaaa gagaatcaac
      121 aaaactaacc ctgtcaaaag cctgtaagtc ggtcatcttc tctcctactc caatatattt
      181 tacaggtatt ttaaactcat cagaaaaacc aatcaccaca ccaccttttg cagtgccatc
      241 taattttgtc agcgcaagtg cattaacttc tgttgcagca gtaaactgtc ttgcctgctc
      301 aatagcattc tgacctgttg aagcatcaag caccaataaa atttcatgag gtgcatcgga
      361 cacaactttc tgcattacac gttttatttt agtcaactca ttcatgagat taactttatt
      421 gtgcaagcga cctgctgtat ctattataac cacatcgctg ttctgagcca cacctgactt
      481 aacagtatca aatgcaaccg aagccggatc ggcattcatt ccttgcgaaa caataggcac
      541 ctgaacacgt tctgcccaaa tgtttaactg atctactgca gcagcacgaa aagtgtcggc
      601 cgcacccagt aacacatttt ttcctgattg tttaaactga tatgccaatt taccaatagt
      661 cgttgtcttg cccacgccat ttacacctac aaccattatg atatagggtt tcttactttc
      721 atcaattaaa ttggccacag tagccgattt gttctcgcca agtaatgcag ctatttcttc
      781 ttttaaaatt gtattcaact gatcggttcc cacatattta tcacgtgcta ccctgtcctg
      841 aattcgtttt acaattttta aagttgtatc cactccaacg tcagacgaga ctaaaatctc
      901 ctctacatta tcgagcactt catcatcaac cgttgattta ccaattactg ctttggtaat
      961 ttttgtaaaa aaattctcac gtgttttatt taatccgctg tcgagtgatt ccttatcttt
     1021 tttcgaaaac agtccgaaaa atgccatatt atgattcttt tattatagta ttaaaacaaa
     1081 aacagccttc ttccttaggg aagaaagctg tcattcgatt tcataaatat tatttctctt
     1141 tcaaaaaatc ttttacgtga tcgttatgca caatctcttc tttgaatgaa taggctccat
     1201 tggcaccttt caccattttt atcaccttgg aaaactcttt tcctttaccg gttttaagtg
     1261 ttgcaactac ttttttagcc attttattta agctttaaat tatttaattt ctttatgaag
     1321 agtaactttt ttcagaatgg ggttgtattt tttcaactcc aaacgctctg tagtgttctt
     1381 cttgttcttt gttgtgatat aacgtgatgt tccgggcata ccgctgttct tatgctctgt
     1441 gcactccatg ataacctgaa ctctgtttcc tttttttgcc atgatgctaa aagattaaat
     1501 gtgtcctttt tttatcagac tgtttataca ggctgaaatt ccttttttat tgatggtacg
     1561 gattgcagaa gcagatactt ttaaagtaac ccactcaccg gtttccggaa taaaaaactt
     1621 ttttgtttgc agatttggat tgaatctgcg ctttgtttta cgattggaaa aagaaatatt
     1681 gtttccaaca atacttgtct ttccggtcaa atcacatata cgggacataa ctactcaatt
     1741 ttattaaggg agtgcaaaag taattatttt ttgtttaatg ccaaatgctt ttcataattt
     1801 tttactacag caaccatttc tttgctttac aagacaataa actataaatc aattatttaa
     1861 cttattaaat taagcactat tc
//
LOCUS       DAOOOI010000213         1829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_9458_length_1829_cov_7.811725, whole genome shotgun sequence.
ACCESSION   DAOOOI010000213 DAOOOI010000000
VERSION     DAOOOI010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9458_length_1829_cov_7.811725"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="PKX59_12000"
     CDS             complement(<1..1563)
                     /locus_tag="PKX59_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOZ91620.1"
                     /translation="MFFKTVILQQRSSGGGSTITQQLAKNLFPRENESGFRLAVRKVK
                     EWIIAVRLERNYTKQEIMAMYLNTVEFGHNSYGIKSAAKTFFNKMPSQLKVEESAMLV
                     GLLQAPTRYSPVRNPKNALIRRNTVLSQMEKYKFLSEAAYDSIVKLPIKLNFRAEDHN
                     DGLAPYFRETLRLELVKWCKEHKKSDGSSYNLYRDGLRIYTTIDSRLQEYAEEGMKEH
                     LTELQKTFYQHWKGRVPWAQHPEVIEDGIKKSDRYISLKAEGLSEQEIKENFATKIPM
                     TIFSWKGDIDTMMSPLDSLKYYKMFLQAGVMSIEPQTGYVRAWVGGNNYRYFKYDHVK
                     AGRRQVGSTFKPFLYTLAMQEGYSPCFKVPNVRVTIPIPGQPDWSPSNSDGKYGGMLT
                     LKEALAESVNSVSAYLMKQFGPKPMIEIARKMGITSPIDEVPSICLGTPDVSLFEMCG
                     AYSTFANKGVWTEPIYITRIEDKNGMVLQEFVPRKVEAISEETAYLMLNLMQGVVQSG
                     TGGRLRFRFGLTN"
     gene            1585..>1829
                     /locus_tag="PKX59_12005"
     CDS             1585..>1829
                     /locus_tag="PKX59_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91621.1"
                     /translation="MNITGMTVVAGRLANGPEHLKFGLNINGTGMIEGVQMDTLPKEC
                     DGDPLNGIPNAVPYTQTWDITDFNSLTPVTFTIGCFGG"
BASE COUNT          489 a          486 c          331 g          523 t
ORIGIN      
        1 atttgtcaat ccaaaacgaa aacgcaaacg tccacccgtt ccactttgta caacgccctg
       61 catcagattc agcatcagat aagctgtctc ttcgcttatg gcttcaacct tgcgtggcac
      121 aaactcttgc agcaccatgc cgtttttatc ttcaattcgt gtaatgtaaa ttggctctgt
      181 ccacacacct ttattggcaa atgtgctgta tgcaccacac atctcaaaca aactcacatc
      241 aggtgtgcct aaacaaattg aaggcacttc gtcaatgggc gatgtaatac ccattttcct
      301 tgcaatctct atcatcggtt ttggtccaaa ctgtttcatc agataggccg acacactatt
      361 caccgactcg gcaagagctt ccttcaatgt cagcatgccg ccatattttc catcactgtt
      421 cgatgggctc cagtcgggct gtccgggtat aggaatcgtc acacgcacat tagggacttt
      481 aaagcaaggc gaatagcctt cctgcatggc aagcgtatat aaaaacggtt taaatgttga
      541 acccacctgc ctgcgtcctg ccttcacgtg gtcgtattta aagtagcgat aattgtttcc
      601 gccaacccat gcacgcacat agcctgtctg tggctcaata ctcatcacac cggcttgcaa
      661 aaacatcttg taatacttca gcgagtcaag cggactcatc attgtatcaa tatcgccctt
      721 ccacgaaaaa atcgtcatcg gtattttagt ggcaaaattt tcttttatct cctgctcact
      781 caacccttca gctttaagcg aaatatagcg gtcgcttttc ttaataccgt cctctataac
      841 ttccggatgt tgcgcccacg gcactcgacc cttccaatgc tgataaaaag ttttttgcaa
      901 ctctgtcaga tgttccttca tcccttcttc ggcatactcc tgcaatcgcg aatctatcgt
      961 agtgtaaatg cgcaaaccgt cacgatacaa attatagctg ctgccgtcag attttttatg
     1021 ctccttgcac cacttcacca gttccaatcg caaagtctct cggaaataag gtgccagtcc
     1081 gtcattatga tcctctgccc tgaaatttaa ttttatcggc agcttcacta tagagtcgta
     1141 tgctgcctca ctcaaaaatt tatacttctc catctgactc aacaccgtgt tgcgtctaat
     1201 caatgcattc ttcgggttac gcaccgggct gtaacgtgta ggtgcctgca gcaaacccac
     1261 cagcatggcc gactcttcca ccttcaactg agatggcatc ttattaaaaa aagtctttgc
     1321 tgccgatttt attccatagc tgttatgacc aaattcaacc gtattcagat acatggccat
     1381 aatctcctgc ttggtatagt tgcgctccag tcgcacagca ataatccact ccttaacctt
     1441 gcgcactgct aaccgaaaac cgctctcatt ctcacgtgga aacaagttct tggccaactg
     1501 ttgtgtaatt gtgctgccac cacccgaaga acgctgctgc aaaatcaccg tcttaaaaaa
     1561 caccctcccc aatcaggtta tcaaatgaat ataacgggga tgacagttgt agccggaaga
     1621 cttgcaaatg gccctgagca tctaaaattt ggtttaaaca ttaacggtac aggaatgata
     1681 gaaggcgttc aaatggacac cttacctaaa gaatgtgatg gcgatccttt aaatggaatt
     1741 cctaatgctg ttccttatac acaaacatgg gatattaccg attttaattc tcttacacct
     1801 gttactttca caataggatg cttcggagg
//
LOCUS       DAOOOI010000214         1723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_10108_length_1723_cov_12.308753, whole genome shotgun
            sequence.
ACCESSION   DAOOOI010000214 DAOOOI010000000
VERSION     DAOOOI010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10108_length_1723_cov_12.308753"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PKX59_12010"
     CDS             <1..470
                     /locus_tag="PKX59_12010"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOZ91622.1"
                     /translation="PETKDSEFTWKDFQARVKKEWTIEPIEINLADEEEWQKLPAIGP
                     GRAAAIVSYRNKLGGFTSINQLHEVNAIVDSVFEKISPYLLCNIKPIQKKNINTDDAF
                     SLRHPYISSSLATIIVNYRKMHGDYKSTEQLRDLKIIKPEDFEKLAPYIVVGF"
     gene            595..1167
                     /locus_tag="PKX59_12015"
     CDS             595..1167
                     /locus_tag="PKX59_12015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOZ91623.1"
                     /translation="MMDYFKHESSYVDDGCTIGKGTKIWHFSHIMPGCRIGEGCNIGQ
                     NVVISPDVVLGNNVKVQNNVSLYTGVVCEDDVFLGPSMVFTNVTNPRSAVNRKNEYKK
                     TVVKKGASIGANATIVCGHDIGRFAFIGAGAVVTKDVADYALMAGNPARQMGWMSEYG
                     HRLKFDKDGMAVCPESGEKYLLKNNQVTKI"
     gene            1183..>1723
                     /locus_tag="PKX59_12020"
     CDS             1183..>1723
                     /locus_tag="PKX59_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOZ91624.1"
                     /translation="MSSEKKEKIKFAVIGCGHIGKRHAEMVRRNNECELVAMCDIRQE
                     NEVGIADFNVPFFQSVDAMLTSIPDIDVVCVCSPNGVHAEHSLKALEAKKHVVCEKPM
                     ALSKADCEKIIFKALQVSRHVFCVMQNRYSPPSVWLKEVIDQNIIGEIFMVQLQCYWN
                     RDERYYKKGGWKGTSDMDGG"
BASE COUNT          564 a          285 c          384 g          490 t
ORIGIN      
        1 caccggaaac taaggacagt gagtttacct ggaaagattt tcaggctaga gtaaaaaaag
       61 aatggactat tgagcctatt gaaattaatt tagctgatga ggaagagtgg cagaagttgc
      121 ctgctattgg tcctggcagg gcagcagcta tagtttcgta tcgtaataaa ttaggtggtt
      181 ttacatcaat taatcaattg catgaagtca atgctattgt tgattctgtt tttgaaaaaa
      241 tcagccctta tttactttgt aatataaagc ccattcaaaa gaagaacatc aataccgatg
      301 atgcatttag cctcagacat ccatacatca gcagcagtct ggcaactata attgtaaact
      361 acagaaaaat gcatggtgac tataaatcaa cagaacaatt acgtgattta aaaataatta
      421 aaccggaaga ttttgaaaaa cttgcaccct atatagttgt gggtttttag cagcaaaaaa
      481 ctacggccaa actcaaaaat gtaagataat gaccatctta atctaaaaac agactatcat
      541 tcttaacaaa attaaagtaa atttgcaccc ctattaaagc gggttaagga agcgattatg
      601 gattatttta aacatgagtc gtcctatgtt gacgatggat gtaccatagg aaagggtacc
      661 aagatatggc acttttcaca tattatgccc ggttgtcgca ttggcgaagg ttgtaacatc
      721 ggtcagaatg tggttatctc tcctgatgtt gttctgggca acaatgttaa ggttcagaac
      781 aatgtttcac tttatacggg agtagtttgt gaggacgatg tatttctggg tccaagtatg
      841 gtttttacca atgtaaccaa tccgcgcagt gctgtaaacc gcaaaaatga gtataaaaaa
      901 actgtagtga aaaaaggtgc ttccataggc gctaatgcca ctattgtttg tgggcacgat
      961 attggccgtt ttgcctttat tggtgccggt gcagttgtaa caaaagatgt tgctgactat
     1021 gcgttgatgg ctggtaatcc tgcccgtcaa atggggtgga tgagtgaata tggtcatcgc
     1081 ctgaagtttg ataaagatgg aatggcagtt tgcccggaaa gcggagaaaa atacctgctg
     1141 aaaaataatc aggtaacaaa aatttagaaa gaccgcactt ttatgagctc agaaaaaaaa
     1201 gaaaaaataa aatttgctgt aattggttgt gggcacattg gtaaacgtca tgccgaaatg
     1261 gttagacgca acaacgaatg tgagcttgtt gccatgtgcg acatcagaca ggaaaacgaa
     1321 gtaggtattg ccgactttaa tgttccattt tttcagtctg ttgatgccat gctcaccagc
     1381 attcctgata ttgatgtagt gtgtgtttgc tcacccaatg gtgttcatgc cgaacattca
     1441 ctaaaagcat tagaagcaaa aaaacatgtg gtttgcgaaa aacctatggc actgtcaaaa
     1501 gccgattgcg aaaaaattat ttttaaagca ctgcaggtat ctcggcatgt gttttgtgta
     1561 atgcaaaacc gctattcacc gccatcagtt tggcttaaag aggtaataga tcaaaatatt
     1621 attggcgaaa tttttatggt gcagttgcaa tgctattgga accgtgatga gcgttactat
     1681 aaaaaaggcg gatggaaagg tacaagtgac atggatggcg gaa
//
LOCUS       DAOOOI010000215         1711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_10189_length_1711_cov_4.588164, whole genome shotgun sequence.
ACCESSION   DAOOOI010000215 DAOOOI010000000
VERSION     DAOOOI010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10189_length_1711_cov_4.588164"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /gene="rho"
                     /locus_tag="PKX59_12025"
     CDS             <1..1155
                     /gene="rho"
                     /locus_tag="PKX59_12025"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOZ91625.1"
                     /translation="QQNNFRQQNDYFEFDNAITAEGVLEIMPEGYGFLRSSDYDYLSS
                     PDDVYVGQSQIKAFALKTGDTVKCKVRPPREGEKFFPLTKVELINGLEPTVVRDRIPF
                     DYLTPLFPFEKLNLAGENSTMSTRIMDMFSPIGKGQRGLIVAQPKTGKTVLLKEVANA
                     IAANHPEVYLIVLLIDERPEEVTDMARSVKAEVISSTFDEPADKHVKIANLVLEKAKR
                     LVECGHDVVIVLDSITRLARAYNTTAPASGKILSGGVDSNALHKPKRFFGAARKIEKG
                     GSLTIIATALIDTGSRMDEVIFEEFKGTGNMELQLDRKLSNKRVYPAIDLVASSTRRE
                     DLLLDRKMLERMWILRNHLADMNPMEAMEFLLDRLRGTKSNEEFLISMNS"
     gene            complement(1303..>1711)
                     /locus_tag="PKX59_12030"
     CDS             complement(1303..>1711)
                     /locus_tag="PKX59_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOZ91626.1"
                     /translation="GLDVGKFREDYNGKAKELFNEDLALGKQLGVRGFPTMFFTDTSG
                     KTETVYGSKPYAAFENAIIKLQPDAQKFNYDKSVAAVFANYHSLTAKEYSELTGTARQ
                     QSEQVLNDLVANGTLEKLTTKNGAIWKLKNAGQ"
BASE COUNT          524 a          320 c          344 g          523 t
ORIGIN      
        1 caacagaata atttccgtca gcagaacgac tattttgagt ttgacaatgc cattaccgca
       61 gaaggtgttt tggaaattat gcccgaagga tatggttttt tacgctcatc ggattatgac
      121 tatttaagtt caccggatga tgtgtatgtt ggacagtcgc agataaaagc atttgcatta
      181 aaaacaggcg atacagtaaa atgcaaagtt cgtccaccac gtgaaggaga aaaatttttc
      241 ccgcttacaa aggttgaact gataaacgga cttgaaccta cagttgttcg cgatcgtatt
      301 ccatttgatt atctgacacc acttttccca ttcgagaaac ttaatctcgc aggagagaac
      361 agcaccatga gcacaagaat tatggatatg ttttcgccaa tcggaaaagg ccagcgtggg
      421 ttaattgtgg cacagccaaa aactggtaag acagtacttt taaaagaagt tgccaatgcc
      481 attgctgcca accatccgga agtttatctg atagtattgc tgattgatga aagacctgaa
      541 gaggttacag atatggcacg cagtgtaaaa gccgaagtta tttcttcgac ctttgatgag
      601 cctgcagaca agcatgtcaa aattgccaac cttgttttgg aaaaagcaaa acgtttggtt
      661 gagtgcggac atgatgtagt gattgtattg gattctataa cccgattggc acgagcctat
      721 aacaccactg ctcctgcatc aggaaaaata ttatccggtg gtgttgactc taatgcactg
      781 cacaaaccaa aaagattttt tggtgctgca cgtaaaatag aaaaaggtgg ttcattaacc
      841 atcattgcaa ctgctttaat tgacaccggt tcgcgcatgg acgaagttat ctttgaagaa
      901 tttaaaggta ctggtaacat ggagttgcag cttgaccgca agttaagcaa caaacgtgtt
      961 tatccggcaa ttgacttagt tgcttcaagt acaagacgtg aagatttatt acttgaccgc
     1021 aaaatgttgg agcgtatgtg gattttaaga aatcacttgg ccgacatgaa cccaatggaa
     1081 gcaatggaat ttttacttga ccgattaaga ggcacaaaat caaacgaaga gtttcttatc
     1141 tctatgaaca gctagaagtt tggaaagtta aaaagctgga aggtttggaa ggttgtggaa
     1201 ttggagaatt agagatttga agaatgtgga atgtaaaaat gtatctctat ttactgttac
     1261 atgagtgttg caccatagtt tagacaatta atatcccact tactattgcc ctgcattttt
     1321 caatttccaa attgctccgt ttttagttgt cagtttttct aatgttccat ttgcaaccaa
     1381 atcattaagg acttgttcgc tttgctgtct tgccgttcct gttaattccg aatattcttt
     1441 agcagttaat gaatgatagt ttgcaaaaac tgctgccaca cttttatcat agttgaattt
     1501 ttgcgcatca ggctgaagtt ttataattgc attttcaaaa gcagcataag gttttgaacc
     1561 atatactgtt tctgtctttc ctgacgtatc tgtaaaaaac atagtcggaa aacctcttac
     1621 acctaactgc ttgcccaatg ctaagtcttc attaaatagc tctttagctt ttccattgta
     1681 atcctcccta aacttaccta catctaatcc a
//
LOCUS       DAOOOI010000216         1619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_10830_length_1619_cov_15.992967, whole genome shotgun
            sequence.
ACCESSION   DAOOOI010000216 DAOOOI010000000
VERSION     DAOOOI010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10830_length_1619_cov_15.992967"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PKX59_12035"
     CDS             complement(<1..461)
                     /locus_tag="PKX59_12035"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOZ91627.1"
                     /translation="MKAKTAKESLTIQTEIVLPNDTNVLGNLMGGRLLHWMDVCSAVA
                     AHRHCGRVVVTASVNNVSFNQPIRLGDIVTIQAKVSRSFTSSMEVFMEVWVERNATGD
                     KIKCNEAIYTFVAVDQQGSPIAVPELIAESEEEKKRFDGALRRKQLALILAG"
     gene            575..1300
                     /locus_tag="PKX59_12040"
     CDS             575..1300
                     /locus_tag="PKX59_12040"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOZ91628.1"
                     /translation="MQTKLIALTAIIGMMLNGKMAHSQTPVNMSETRKIEVTGTAEMK
                     VIPDEIFVVITIQEYTENKKKTDINEVRKNFLAACTKAGIAKENISIQGMNGYNNSGW
                     YWQRKKKTPDFVASSSYIIKFNSGEQIERLMSQLDDKGTQNMYISSTSHSKMDEFKKQ
                     VKTEALQAAKAKATYLCEGIGEKVGKVLLIKEVEGTNYAEPMMMKSRMMEASMADSNF
                     EPSGLEFQKITIQMEILAQFAIQ"
     gene            1308..>1619
                     /locus_tag="PKX59_12045"
     CDS             1308..>1619
                     /locus_tag="PKX59_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91629.1"
                     /translation="MQNSKGRKRGNSDKPQAGRYKSEGRPSRGKKFGSSTDKSKEEFV
                     KSGNSYSKNKFSGKRRFDADESGFERKRKNFGSDDNAGFEKRERPSRFKKEGGFEREK
                     RS"
BASE COUNT          585 a          291 c          341 g          402 t
ORIGIN      
        1 cctgcaagga ttaaggcaag ttgcttacgt cttaatgcac catcaaaacg ttttttttct
       61 tcttcgcttt cagcaatcaa ttcaggcact gcaatggggc tgccttgttg gtcaacagct
      121 acaaaggtgt agatggcttc attgcacttt attttatctc ctgtggcatt acgttcaacc
      181 caaacttcca taaaaacttc catgcttgaa gtaaaggagc gcgatacttt tgcctgaatg
      241 gtaacaatat cgcccaaacg gataggttga ttaaacgata cattgtttac agaagctgta
      301 accactacac gtccacaatg acggtgtgca gctacggcag aacaaacatc catccaatgc
      361 agcaggcggc ctcccatcag attgccaagt acgttggtgt cgttaggaag aacaatctca
      421 gtttgtatgg ttaaactctc tttagcagtt tttgctttca taatctgatt ttttgcggct
      481 gcaaatatct gaataattaa acttaaaatg aacgaatcaa agccacactg tggaattaaa
      541 cctatctttg catccacatt ggaaaacaat taaaatgcaa acaaaactaa tagcactgac
      601 agccattatc ggaatgatgt taaacggtaa aatggcacat tcacaaaccc cggtcaatat
      661 gagtgaaaca agaaaaattg aagtcacagg aactgctgaa atgaaagtaa tacctgatga
      721 aatttttgtt gtcataacca ttcaggaata tacagagaat aagaagaaga cagatataaa
      781 tgaggtgcga aaaaattttc ttgcagcatg cacaaaagcc ggtatagcta aagaaaatat
      841 ttcaatacaa ggaatgaatg gctacaataa cagtggttgg tactggcagc gcaaaaagaa
      901 aactcccgat tttgttgctt cttcaagtta tataattaag tttaattcag gtgagcagat
      961 tgaaagatta atgagtcagc ttgatgacaa aggcacgcag aacatgtaca tcagttccac
     1021 ttcacacagt aagatggatg agtttaaaaa acaagtaaaa acagaagcat tacaggcagc
     1081 caaagctaag gccacctatc tttgtgaagg aattggagaa aaggttggca aggtattact
     1141 gataaaagaa gttgagggca caaattatgc agaacctatg atgatgaaaa gccgaatgat
     1201 ggaagcttca atggcagatt ctaattttga accttcggga cttgaattcc aaaagataac
     1261 catacagatg gaaatattgg cgcaatttgc catacaataa gcaacacatg cagaacagta
     1321 aaggccgtaa gcgcggcaac agcgacaagc cgcaagccgg cagatacaaa tctgaaggac
     1381 gtccttcaag aggaaaaaaa ttcggaagta gcactgataa atccaaagag gagtttgtaa
     1441 agtcaggaaa cagttattca aaaaataaat tttcaggcaa acgcaggttt gatgctgatg
     1501 agtccggctt tgaaagaaaa cggaagaatt ttggaagtga tgataacgct ggtttcgaaa
     1561 aaagagaaag accatcgcgc tttaaaaaag aaggtggttt tgagcgagag aaaagaagt
//
LOCUS       DAOOOI010000217         1581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_11116_length_1581_cov_6.268676, whole genome shotgun sequence.
ACCESSION   DAOOOI010000217 DAOOOI010000000
VERSION     DAOOOI010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11116_length_1581_cov_6.268676"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..865
                     /locus_tag="PKX59_12050"
     CDS             59..865
                     /locus_tag="PKX59_12050"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOZ91630.1"
                     /translation="MKDLEQLTNKTCEAVVKAAQFIQQESKRFDKSVVEYKGLHDLVS
                     YVDKTAEKILVEEFRSILPSAGFITEESTVSKSNNELQWIIDPLDGTTNFIHGLPCYC
                     VSIGLMENNKLQMGIVHEINFNETFYCWKNGGAWLNGKQISVSPTNKLQHALLATGFP
                     YTNYSRMEEYMKVFDFCMRNTRGLRRLGSAAADLAYTACGRLDGFFEYGLNAWDVAGG
                     ALLVEEAGGVVADFSGEKDFIFGQEIVACNKAVFDEFMEVIYTSFRNKND"
     gene            883..>1581
                     /locus_tag="PKX59_12055"
     CDS             883..>1581
                     /locus_tag="PKX59_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOZ91631.1"
                     /translation="MQHVAQTSQAPLALHIVRADGCFLYDDKGKEYIDLIAGISVCNL
                     GHNNSAINEAVKKQIEQYSHIMVYGELIESPQTLFASLLASHLPPNLNCTYFVNSGTE
                     AIEGAMKLAKRTTGRNEIVALTNSYHGSTQGSLSLMSNEYFSSAFRPLIPGVKFIDPT
                     KPDFDVLNENTSCIIMELVKAEEGCKPLTYDFVNNVVRICREKNILLVVDECQTGLGR
                     TGKAFAFENYDIIPD"
BASE COUNT          513 a          241 c          349 g          478 t
ORIGIN      
        1 ccattagtgt gcctgcagat ttcagtcgca tacagatatc ttaatttttt taaggtcaat
       61 gaaagattta gaacagttaa ccaacaaaac ctgtgaggct gtagttaagg cagcacagtt
      121 tattcagcag gaaagcaaac gatttgacaa atcagttgtg gagtataaag gactacatga
      181 tttagtgagt tatgtggata aaacagcaga aaaaatttta gtagaagagt ttagaagtat
      241 tttaccttca gcaggattta ttacggaaga gtcaacggta tctaaaagta acaatgaatt
      301 acaatggata attgatccgc ttgatggaac aaccaatttt atccacggac taccttgtta
      361 ttgtgtcagt attggattga tggaaaacaa taaactgcaa atgggcattg tgcatgagat
      421 aaattttaat gaaacctttt attgctggaa gaatggtggt gcatggctca atggaaaaca
      481 aatttcagta tcaccaacaa ataagttgca acatgcattg ttggcaacag gatttccata
      541 caccaattat tcaagaatgg aagagtacat gaaagtattt gacttttgta tgcggaatac
      601 aagaggtcta agaagacttg gttctgctgc tgccgatttg gcttatactg catgtggtcg
      661 ccttgatgga tttttcgaat atggtctcaa tgcatgggat gttgcaggag gcgctttatt
      721 agttgaagaa gcaggaggtg tggttgcaga tttttcagga gaaaaagatt ttatttttgg
      781 acaggaaata gtagcctgca ataaggctgt ttttgatgag tttatggaag ttatttacac
      841 ttcattcaga aataaaaatg attaatcacc ggcaactttt ttttgcaaca cgttgcgcaa
      901 acaagtcagg cgccactggc gttgcatatt gtgcgtgccg atggttgttt tttatatgat
      961 gataaaggaa aagaatacat tgatttaatt gccggtattt ctgtatgtaa cttaggtcat
     1021 aataattctg ctatcaatga ggctgtcaaa aaacaaatag agcaatatag tcacataatg
     1081 gtttatggcg aattgataga aagcccgcag accttgtttg caagcttgct tgcatcgcat
     1141 ttaccaccaa atttaaattg cacctacttc gtaaattccg gaaccgaagc catagagggg
     1201 gctatgaagt tagccaaaag aactacagga aggaatgaaa ttgttgcatt aactaattcc
     1261 tatcatggca gcacacaagg ctcattgagt cttatgagta atgaatattt ttcatcagcg
     1321 ttcaggcctt taataccggg tgttaaattt attgacccga caaaacctga ctttgatgtg
     1381 ttgaacgaaa acacatcgtg tatcatcatg gaattggtga aggccgaaga aggatgtaag
     1441 ccactaacat acgattttgt aaataatgtt gtgcgaattt gcagagagaa aaatattctg
     1501 ttagttgttg atgaatgtca gaccggattg ggcagaacag gtaaggcgtt tgcctttgaa
     1561 aattatgata ttattcccga t
//
LOCUS       DAOOOI010000218         1560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_11275_length_1560_cov_36.055814, whole genome shotgun
            sequence.
ACCESSION   DAOOOI010000218 DAOOOI010000000
VERSION     DAOOOI010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11275_length_1560_cov_36.055814"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /locus_tag="PKX59_12060"
     CDS             complement(<1..1447)
                     /locus_tag="PKX59_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HOZ91632.1"
                     /translation="MASQFIPVDFNPFEEVIPDNFFTTSEEQREIWTSVQIGGDSASL
                     AYNESVTLFLKGLCNHKHLSEACKNVVQRHQSLRSTFNTSGETFQVHEHVNLEIPYID
                     LTDLSPSDKDARILHFSQREVSIPFDLLNGPVIRFAIFKISNSETALVITAHHIVCDG
                     WSIGIIMQDISKFYSALANNTQPDLEPVVPFSTYVKRQEERKLNGEYKRTEDYWLNLY
                     SADVPTVDLPVNKARPAARTFSAKRFDITIDAGLVSDLRKLGAKNGSSYVNTLFAAFE
                     VFLFRLTSNPSLVLGLPAAGQSASGMYAMVGHCVNLLPIKSLVDENITFGEYLKARKP
                     LMFDAFDNQEFTMGSLLGKLPLQRDPSRIPLVPAVFNVDLGMTQGVSFEGLNYEYTSN
                     ARKFENFELFVNATGMGDKLIIECTYNTDLFEDEMMQLRMQEFVVLLNGLVEYVDSAI
                     KNLPLLTDDEKRKLFIEWPVVKENKLSNECIH"
BASE COUNT          513 a          310 c          267 g          470 t
ORIGIN      
        1 tatgaatgca ctcattgcta agtttattct cttttacaac tggccattcg ataaacaact
       61 ttcttttttc atcgtcagtc agtaatggca aattttttat tgcactatca acatactcaa
      121 ccaaaccatt aagaagcact acaaactcct gcattctcaa ttgcatcatc tcatcttcaa
      181 aaaggtcggt attgtaagta cattcaataa tgagtttatc gcccataccg gttgcattca
      241 caaacaattc aaagttttca aactttctgg catttgaagt atattcatag ttcaatcctt
      301 caaaagaaac tccttgagtc atacccaagt caacattgaa cacagcaggc actaatggaa
      361 ttcttgacgg gtcgcgttgc agcggaagtt ttcctaacaa acttcccatt gtaaattcct
      421 gattatcgaa agcatcaaac atcaaaggtt ttctggcttt caaatattca ccgaaagtga
      481 tgttctcatc aacaagactt ttaattggta aaagattaac acaatgacca accatagcat
      541 acattccgga cgctgactgt cctgcagcag gaagtcctaa taccaatgaa ggattggatg
      601 ttaaacgaaa aagaaacact tcaaatgcag caaacaacgt atttacataa ctgcttccat
      661 tttttgcacc aagttttctt aaatcactaa ccaaaccggc atcaatagtt atatcaaatc
      721 gttttgcact gaaggtacgt gcagcagggc gagctttgtt aaccggaagg tcaacggtag
      781 gaacatctgc ggaatataaa ttgagccaat aatcttctgt acgtttatat tccccattca
      841 atttcctttc ttcctgacgc tttacataag tgctgaaggg tacaacaggt tcaagatcag
      901 gttgcgtatt atttgctaaa gcagaataaa atttactgat atcctgcatg atgataccaa
      961 tactccatcc atcacagaca atgtgatgag ctgtaataac caatgccgtt tctgaatttg
     1021 aaattttaaa aatggcaaaa cggatgacag ggccattcaa caaatcgaaa ggtatggaca
     1081 cttcacgctg cgaaaaatga agtatccgtg catctttatc tgacggactt aagtcggtta
     1141 aatcaatata tggaatttca agattgacat gttcatgtac ctgaaatgtt tcacctgaag
     1201 tattgaaagt agagcgaagt gactgatgcc tttgaacaac attcttgcaa gcttctgaaa
     1261 ggtgcttgtg attacacagt cctttcagaa aaagtgtaac actttcgtta taggctaatg
     1321 atgcagaatc acctcctatt tgaacggatg tccagatttc acgttgctct tctgatgtgg
     1381 taaaaaaatt gtccggaata acttcttcaa aagggttaaa atctaccggg ataaattgcg
     1441 atgccatctt cctttgtcat tggtggttta ataaatacaa tacgaaaata ttataaaata
     1501 gtttccttta gcaataaatt gttaagcgat atgaaaataa gggtgaatgt agccattcac
//
LOCUS       DAOOOI010000219         1470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_11996_length_1470_cov_13.848763, whole genome shotgun
            sequence.
ACCESSION   DAOOOI010000219 DAOOOI010000000
VERSION     DAOOOI010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11996_length_1470_cov_13.848763"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1470
                     /locus_tag="PKX59_12065"
     CDS             <1..>1470
                     /locus_tag="PKX59_12065"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOZ91633.1"
                     /translation="ANWFYGALRQQLINYFANDEQRGFIDSLNFRVDDKVVSLVYDSI
                     NIDICKIVLNKPLLPNDTILITTPFRIKIPDAKFSRLGHVGQSYMISQWYPKPAVYDK
                     DGWHTMPYLSQGEFYSEFGSFLVDITVPANYVVAASGYLKTPSEVEFINKKIKTESNQ
                     INFDKQNTFPESESSSKSIRFALDSVHDFAWFADKRFRIVSKVIKLASGHEVECRAYY
                     TPANSYVWHRAADYVAEAVLFYSKYVGEYPYGVCTAVDGTIAAGGGMEYPTITVIGST
                     GNSMVLNQSIAHEVGHNWFYGILASNERKLPWMDEGINSFYEQLYMIQYYARESAAAY
                     QLTGVPLKIAKTFGADKMSFAGFNKISYLLTARTNDDQSSGLTSEYFSNLNYGADIYS
                     KIPLCFSQLRDFLGHEKYDSCMQTYFNTWKFRHPDEAAIQNVFETTSGKSLGWFFNGM
                     IDSNSKSDAKICKVNTDSGGTDVLLKNRGNLAMPVNVSF"
BASE COUNT          460 a          206 c          307 g          497 t
ORIGIN      
        1 tgctaattgg ttttacggag cattacgtca gcaactgatt aattattttg caaatgatga
       61 gcaaagagga tttattgata gtctgaattt tagagttgat gataaagttg tgtcattagt
      121 ttatgattcc attaatattg atatatgtaa aattgttctg aataaaccat tattgcctaa
      181 cgacacaatc ttaattacaa caccattcag aataaaaatt cctgatgcaa agttttcaag
      241 acttggtcat gttggacagt cttacatgat ttcgcaatgg tatcctaagc ccgcagttta
      301 tgataaagat ggatggcata ctatgcctta tttatctcag ggagaatttt attctgagtt
      361 cggttctttt ctggtggata taactgtgcc tgcaaattat gttgtagcag cttcaggcta
      421 tttaaaaact ccttcagaag ttgagtttat taataaaaaa ataaagacag aaagcaatca
      481 aattaatttt gataaacaaa atacttttcc tgaatcagaa agttcaagta agtcaattcg
      541 ttttgcactt gacagcgtgc atgattttgc ctggtttgca gataagcgat tccgtattgt
      601 atcaaaagtt ataaagttag cttccggaca tgaggtggaa tgtcgtgcat attatacacc
      661 tgctaattct tatgtctggc atcgtgctgc tgattatgtt gctgaagcag ttttgtttta
      721 ttctaaatat gttggagaat atccttatgg tgtttgtact gctgttgatg gaacaattgc
      781 cgcaggcgga ggaatggaat atccaacaat aacggtaatt gggagtactg gtaatagtat
      841 ggtcttgaat caaagtattg cccatgaagt ggggcataat tggttttatg gcatacttgc
      901 aagcaatgaa agaaagttgc cttggatgga tgaaggtata aattcgtttt atgagcaatt
      961 gtatatgata cagtattatg caagggaaag tgctgcagca tatcagttga ctggagtgcc
     1021 attaaaaatt gcaaagactt ttggtgcaga taaaatgtcg tttgcggggt ttaataagat
     1081 tagctattta ctcacagcca gaaccaatga tgaccaaagt agtggactta caagtgagta
     1141 tttttcgaat ctaaactatg gtgctgatat ttattctaaa atacctttat gtttttcaca
     1201 actcagagat tttttagggc atgagaaata tgattcctgt atgcagactt attttaatac
     1261 ttggaagttc agacaccctg atgaagcagc aattcaaaat gtgtttgaga ctacttccgg
     1321 aaaatcgtta ggttggtttt ttaacggaat gattgatagt aattcgaaat cagatgctaa
     1381 aatctgtaaa gtgaatactg attcaggagg gactgatgta ttgctaaaga atagaggaaa
     1441 tcttgctatg cctgttaatg tgtcttttta
//
LOCUS       DAOOOI010000220         1383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_12836_length_1383_cov_9.122741, whole genome shotgun sequence.
ACCESSION   DAOOOI010000220 DAOOOI010000000
VERSION     DAOOOI010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12836_length_1383_cov_9.122741"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PKX59_12070"
     CDS             complement(<1..453)
                     /locus_tag="PKX59_12070"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOZ91634.1"
                     /translation="MSQKRFAIIVAGGSGTRMQADVPKQFLLLEGRPVLMYSMECLHR
                     VGAEITVVLPDYEQSHWQSLINEHNFNVPHKIAAGGLTRNRSVFNGLQTIEDREALVA
                     VHDAARPLLKPELAEKLFELAVHRGNAIPVIPVRESLRRINSTENKAVN"
     gene            complement(467..>1383)
                     /gene="queA"
                     /locus_tag="PKX59_12075"
     CDS             complement(467..>1383)
                     /gene="queA"
                     /locus_tag="PKX59_12075"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOZ91635.1"
                     /translation="IINYFDEGDVMVMNNTKVFPARLYGNKEKTGAKIEVFLLRELNP
                     ESKLWDVLVDPARKIRIGNKLYFGEDDMLVAEVIDNTTSRGRTLRFLFDGTSADFREV
                     IEKLGNTPLPKYIKRKAEPEDKERYQTVYAKNEGAVAAPTAGLHFSKQLLKRLEIKGI
                     NLAELTLHVGLGTFRPVEVEDLTKHKMDSEWFSITQPCVDIVNKAKAGKHRICAVGTT
                     SMRAMESSVSTLGELKPFEGWTNKFIFPPYDFSLANCMVTNFHMPESTLLMMVSAFAG
                     YDLTMHAYKLAMKEKYRFYSYGDAMLII"
BASE COUNT          382 a          304 c          272 g          425 t
ORIGIN      
        1 gttaacggcc ttattctccg tagaattaat acgtctcagc gactctctga caggtataac
       61 gggtatggca ttgcccctgt ggacagcaag ttcaaagagt ttttcagcaa gttcgggctt
      121 taaaagtggc cgtgctgcat catgcactgc aaccaaagct tctctatctt caatggtctg
      181 taaaccgtta aataccgaac ggtttctggt caggcctcct gctgcaattt tatgtgggac
      241 attaaaatta tgctcattaa taagcgactg ccaatgactt tgttcataat cgggtaaaac
      301 cactgtaatt tctgctccaa cacgatgcag acattccatt gaatacatga gtaccggtcg
      361 tccttcaagc agcagaaact gtttgggaac atcagcctgc attcgtgttc cactgcctcc
      421 ggcaactatt attgcaaatc gcttttggct cataataaat aaactgtcag attatcagca
      481 tggcatcgcc atagctatag aagcggtatt tttctttcat cgctaatttg taagcatgca
      541 tggtaaggtc gtaacctgca aaagcagaaa ccatcatcaa caaagttgat tcaggcatgt
      601 gaaaattagt caccatacaa ttggcaaggc tgaaatcata aggtggaaaa ataaatttat
      661 ttgtccagcc ttcaaaaggt ttaagctcac ctaatgtgga aactgatgac tccattgcgc
      721 gcattgacgt tgttcctact gcacaaatac ggtgtttgcc tgctttggct ttattaacta
      781 tgtcaacaca aggttgtgta atactgaacc attcagaatc catcttatgt ttggttaggt
      841 cttctacttc taccggtctg aatgtgccta aaccaacatg cagtgtcagt tcagcaaggt
      901 tgatgccttt aatttctagg cgctttaata attgtttgct gaagtgtagt ccggcagtgg
      961 gtgctgcaac agcaccttcg tttttggcat atactgtctg gtagcgctct ttatcttcgg
     1021 gctcggcctt tcgtttgata tatttcggga gtggtgtatt tcctaacttc tcaataacct
     1081 ctctgaaatc ggcacttgtg ccatcaaata agaagcgcaa agtacgacca cgtgaagttg
     1141 tgttatcaat tacttcggca acaagcatat catcttcacc gaaatacaat ttattaccaa
     1201 tacgtatttt acgtgcaggg tcaacaagca catcccaaag tttgctttcc ggatttagtt
     1261 cacgtaataa aaagacttca attttagcac ccgtcttttc tttattgcca taaaggcgtg
     1321 caggaaaaac cttggtgtta ttcatcacca tcacatcgcc ctcgtcaaag taattaatga
     1381 tgt
//
LOCUS       DAOOOI010000221         1332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_13327_length_1332_cov_8.061081, whole genome shotgun sequence.
ACCESSION   DAOOOI010000221 DAOOOI010000000
VERSION     DAOOOI010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13327_length_1332_cov_8.061081"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..852
                     /locus_tag="PKX59_12080"
                     /pseudo
     CDS             11..852
                     /locus_tag="PKX59_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(975..>1332)
                     /locus_tag="PKX59_12085"
     CDS             complement(975..>1332)
                     /locus_tag="PKX59_12085"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOZ91636.1"
                     /translation="AVQLATYFGEYMPATQQFVMVDLPIDSKYTNRSYSIASAPSADN
                     IFELCIVLNPKGMGTPYMWENYKVGTTIKLSRVLGKFQIPEPIDTDLCFICTGTGIAP
                     LRSQVVDILNRKIRKP"
BASE COUNT          438 a          239 c          255 g          400 t
ORIGIN      
        1 ctattaaact atgcgtcagt taaaaattac gaaacaagtt accaaccgcg aaacctattc
       61 attagacaaa tacctcagcg agattggtaa gttgccatta ctgacatcgg aagaagaagt
      121 aaggtatgca cggaaaatta aagaaggcga tcagcatgct ttggaaatgc ttacaaaatc
      181 taaccttcga tttgtggttt ctgttgctaa gcaatatcag aattaataaa cgaaggtaac
      241 cttggcctga taaaagccgc ttatcgtttt gatgaaaccc gtggttttaa atttatttct
      301 tatgcagttt ggtggattcg ccaaagcatt ctgcaagcta tttccgaaca ggcacgtatt
      361 gtcagacttc ctgtaaataa acttggtttt atcaataaga taaataagac ttatgcacaa
      421 cttgaacagg attttgaaag agagccttca aacgaagaga ttgcaactgt tttgcatgca
      481 aaccttgatg aaataaacga cacacttcgt tcggccggaa aacatatttc tatggatgca
      541 ccactcttgc agggagaaga tggcaatatg cttgatttaa ttcctacaga agacggacaa
      601 agccctgaag tttcactgat gagcgaatcg cttaaaagag aaattgaacg cgcactaact
      661 acattaactc cccgcgaagc agatgttatc agattatatt tcggactaac tcccgaaccg
      721 gcaatgacat tagaggaaat tggagaaaga tttgaactta cacgcgaaag agtccgacag
      781 ataaaagaaa aggctattcg cagattgaaa catacctcca gaagtaaaat tctgaaggct
      841 tatttaggat gatttttcat tgtatatggt taatgacggt attctttaat tagggtaccg
      901 ttttttattg ttttttgttt agcgatttta tttattttgg tcgcgaaaat tttaatcatt
      961 ctaacacaaa gaatttatgg cttacgaatt ttacgattga gaatatcaac tacctgcgag
     1021 cgtaatggtg caatacctgt acctgtacag ataaaacaaa gatcggtgtc aataggctca
     1081 ggaatctgaa atttgcctaa tactctggaa agctttattg ttgttcctac tttgtaattc
     1141 tcccacatat atggtgttcc cataccttta gggtttagca ctatacaaag ttcaaaaata
     1201 ttgtctgctg aaggtgctga tgcaatagaa tagcttctgt ttgtatattt agaatcaata
     1261 ggcaaatcaa ccatcacaaa ctgttgcgtt gccggcatat attctccaaa ataagtagct
     1321 aattgtactg cc
//
LOCUS       DAOOOI010000222         1323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_13414_length_1323_cov_8.167981, whole genome shotgun sequence.
ACCESSION   DAOOOI010000222 DAOOOI010000000
VERSION     DAOOOI010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13414_length_1323_cov_8.167981"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..>814)
                     /locus_tag="PKX59_12090"
     CDS             complement(4..>814)
                     /locus_tag="PKX59_12090"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ91637.1"
                     /translation="NNFVKCEAVIASVPSSFNPAVARLSANNKQSLILTQPSNINFKA
                     NSEHVYMLSPSTYTQCMVAAKEIATRFPDSKYVLLARDVKPDKDIAQIFKDHLPAKRL
                     KHFITSTVNPDSVCKILKTENAVLIVTSSAEAYVNTILNQVNACIEKPVFAIGLPTWE
                     NFESIDFSILKNLRIAFFTSTYLDYHAASTKSFRNNFINRYKTEPLLNAFQGYEAVME
                     YVKSNTSHGTLRAGYLPTLNIKYVKDAANGIKENQGINLIEIKDYSLIPLE"
     assembly_gap    815..824
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<825..1264)
                     /locus_tag="PKX59_12095"
     CDS             complement(<825..1264)
                     /locus_tag="PKX59_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91638.1"
                     /translation="MTSVQSHLQQLNGNRIMGFSSSKKYFSWALALLLMAGCSSSRKT
                     AQMPPPAPAPKPVEQPVKKPEVVHEKTEKKEFNIGLMLPLNLEQQMQVFDDTSGSAMP
                     DYNNLSNLQFAEGALLAADSLKQLGINIKLNIAETPPDSFALWR"
     gene            complement(1221..>1323)
                     /locus_tag="PKX59_12100"
     CDS             complement(1221..>1323)
                     /locus_tag="PKX59_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ91639.1"
                     /translation="LSIVSNEIINKVKGINRVVYDISSKPPATIEWE"
BASE COUNT          373 a          257 c          244 g          439 t
ORIGIN      
        1 gcactattct aatggaatta atgaataatc tttaatttca ataagattta ttccttgatt
       61 ttctttgatg ccattggctg catctttaac atatttaatg tttaacgtag gcaaatagcc
      121 cgccctaagt gtgccatgtg atgtatttga tttcacatat tccattacag cttcatagcc
      181 ctgaaaagca ttcaataaag gttcggtttt atagcgatta atgaaattgt ttctgaaact
      241 ttttgttgaa gctgcatgat aatctaaata cgttgatgtg aaaaaagcaa tgcgcaaatt
      301 cttaagtatg ctaaagtcta tgctttcaaa attttcccat gttggcagtc caatcgcaaa
      361 tacaggtttt tctatgcagg cattaacctg attgagtatg gtattgacat aagcttcggc
      421 agatgatgtt actatcagaa cagcattttc ggttttcaat atcttacaaa cggaatcggg
      481 gttcacggta cttgtgataa aatgcttcag gcgttttgcc ggaagatgat ctttaaaaat
      541 ctgtgcaatg tctttatccg gtttaacatc gcgcgcaagc agcacatatt tggaatcggg
      601 aaaccgtgtt gcaatttctt tagctgccac catgcattgt gtgtaggttg acggtgagag
      661 catataaaca tgttcgctat ttgctttgaa atttatgttt gatggttgtg taagaatcag
      721 gctttgttta ttgttggcac tcagacgagc aacggcagga ttaaacgatg aaggcacaga
      781 ggcaatcact gcttcacact ttacaaaatt attcnnnnnn nnnnaaacgc cacagggcaa
      841 aactatcggg aggagtttca gcaatgttca atttaatatt gattcctaat tgttttaaac
      901 tgtctgctgc cagtagtgca ccctctgcaa actgcaggtt gctcaaatta ttataatctg
      961 gcatagcact tcctgaagta tcatcaaaca cctgcatctg ttgttccaga ttcaatggca
     1021 acatcaaccc aatattaaac tctttcttct cggtcttttc atgaaccact tcaggttttt
     1081 tcacaggttg ttcaacaggc ttaggtgcag gtgccggtgg tggcatttgc gcagttttcc
     1141 tactgctgct gcaacctgcc atcaacagta atgccaatgc ccaactaaaa tactttttgc
     1201 tgctactaaa acccataatc ctattcccat tcaattgttg caggtggctt tgaactgatg
     1261 tcataaacaa ccctatttat tccttttacc ttattgataa tttcgttaga aactatactt
     1321 aaa
//
LOCUS       DAOOOI010000223         1250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2627
            NODE_14260_length_1250_cov_4.968201, whole genome shotgun sequence.
ACCESSION   DAOOOI010000223 DAOOOI010000000
VERSION     DAOOOI010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864702
            Sequence Read Archive: SRR5007108
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 27
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 15 of 26
            Pseudo Genes (incomplete)         :: 9 of 26
            Pseudo Genes (internal stop)      :: 4 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14260_length_1250_cov_4.968201"
                     /isolate="AMR_MDS_2627"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..285)
                     /gene="atpC"
                     /locus_tag="PKX59_12105"
     CDS             complement(46..285)
                     /gene="atpC"
                     /locus_tag="PKX59_12105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOZ91640.1"
                     /translation="MFLEIITPDKKVFEGEVTSVSVPGADGKFQMLNNHAPIISTLGK
                     GKVKVKTAKETLEFNVKGGVVEMLKNKVVVLAESV"
     gene            complement(356..>1250)
                     /gene="atpD"
                     /locus_tag="PKX59_12110"
     CDS             complement(356..>1250)
                     /gene="atpD"
                     /locus_tag="PKX59_12110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOZ91641.1"
                     /translation="NYGDDFKHSMEQGGWDLSKVDYNKLTDSKATLVFGQMNEPPGAR
                     ARVALSGLTVAEHFRDGDGTGSGRDILFFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGLMQERITSTKRGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRKIAE
                     LGIYPAVDPLDSTSRILTEEVVGKEHYETAQKVKQLLQRYKELQDIIAILGMDELSDE
                     DKMAVHRARRVQRFLSQPFHVAEQFTGLKGVLVPIEETIKGFNMIMNGEVDEYPEAAF
                     NLVGTIDDAIEKGKKLLAEAK"
BASE COUNT          344 a          276 c          236 g          394 t
ORIGIN      
        1 agctaccctt gtgaggtagc tttttttata atttgtattt ttaatctaca ctgattcagc
       61 taaaacaaca actttgtttt taagcatctc taccactcca ccttttacat taaactcaag
      121 cgtttctttt gctgtcttta ctttaacctt gcctttgcca agtgttgaaa taataggagc
      181 gtggttgtta agcatctgaa atttaccgtc agcaccggga acagagacag acgttacttc
      241 gccttcaaac acttttttat cgggagtaat aatttctaaa aacatttatt tacgaatttg
      301 tacgaatttg agaatgacat gacataacct cactttcgca attatctaat ttaaattatt
      361 ttgcttcagc taacagtttt ttgccttttt caatagcatc gtcaatagta ccaacaaggt
      421 taaatgctgc ttcaggatac tcatcaactt cgccattcat aatcatgtta aagcctttga
      481 tagtttcttc aataggcaca agaacacctt ttaatcctgt aaactgctcg gcaacgtgga
      541 acggctgtga taggaatcgc tgtacccttc ttgcacggtg aacagccatt ttatcttcgt
      601 cactcaactc gtccatacca agaatggcaa tgatgtcctg aagttctttg tagcgttgta
      661 acaactgttt tactttttgt gcagtttcat aatgctcttt acccacaact tcttctgtaa
      721 gaattcgaga ggtagaatcc aatggatcta cagcaggata aattccaagc tctgcaattt
      781 tacggctcaa taccgttgtt gcatcaaggt gggcaaatgt tgtagcagga gctgggtcag
      841 tcaagtcatc agcaggtaca tatacggcct gtactgatgt gatagatcct ctttttgttg
      901 aagtaatacg ctcttgcatg agtcccattt ctgtggcaag tgtaggctga taacctacgg
      961 cagaaggcat acgacctaaa agtgccgata cttctgaacc tgcctgcgtg aaacggaaaa
     1021 tgttgtcaat aaagaaaagg atgtcgcggc cactacctgt gccatcacca tcgcggaaat
     1081 gctcggcaac agtaagtccc gacagggcta cacgtgcacg cgctccggga ggctcattca
     1141 tctgtccgaa aacaagtgtt gcttttgagt cggtaagttt gttgtagtca accttactca
     1201 aatcccatcc gccttgttcc atactatgtt taaaatcgtc accataattg
//
